LOCUS       DAWGRZ010000001        49772 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000001 DAWGRZ010000000
VERSION     DAWGRZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 49772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49772
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.001"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            99..1910
                     /gene="typA"
                     /locus_tag="WHT44_000001"
     CDS             99..1910
                     /gene="typA"
                     /locus_tag="WHT44_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ2532252.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(2320..2410)
                     /locus_tag="WHT44_000002"
     tRNA            complement(2320..2410)
                     /locus_tag="WHT44_000002"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2374..2376),aa:Ser,seq:gga)
     gene            complement(2449..2535)
                     /locus_tag="WHT44_000003"
     tRNA            complement(2449..2535)
                     /locus_tag="WHT44_000003"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2499..2501),aa:Leu,seq:cag)
     gene            complement(2571..2657)
                     /locus_tag="WHT44_000004"
     tRNA            complement(2571..2657)
                     /locus_tag="WHT44_000004"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2621..2623),aa:Leu,seq:cag)
     gene            2746..5121
                     /gene="rnr"
                     /locus_tag="WHT44_000005"
     CDS             2746..5121
                     /gene="rnr"
                     /locus_tag="WHT44_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ2532253.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAAKKIPVHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKVGRNYRLVVAIADVSHYVRPDDVIDADAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPDVERLCMVCDMVVTYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWISDGIDHPYKAQIDTLYKLFKILQKKRFERGAVEFESVETQMIFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALVEQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESRKKAKKPVPIKVKKRKGKS"
     gene            complement(5460..6923)
                     /gene="guaB"
                     /locus_tag="WHT44_000006"
     CDS             complement(5460..6923)
                     /gene="guaB"
                     /locus_tag="WHT44_000006"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ2532254.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(7124..7204)
                     /locus_tag="WHT44_000007"
                     /pseudo
     CDS             complement(7124..7204)
                     /locus_tag="WHT44_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(7250..7711)
                     /locus_tag="WHT44_000008"
     CDS             complement(7250..7711)
                     /locus_tag="WHT44_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532255.1"
                     /translation="MNIIMDSTRKFGILWEENSECNGFIYGKIQIIIGENIYPKICPY
                     GYFTLNAVFNSLKSSFEEKYYAGGNNGLDFGEQLFDIDKYNSLELCNIFSIDTTYMSG
                     GGNCEIDCLVLEMGYSGEEERLFYSFDNGKNFKEIRYKKGTVESVIFQLNL"
     gene            complement(7802..10360)
                     /gene="glnD"
                     /locus_tag="WHT44_000009"
     CDS             complement(7802..10360)
                     /gene="glnD"
                     /locus_tag="WHT44_000009"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ2532256.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTVDKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNPENRRRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIIGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTGEESDLLAWLVENHLLMSAVAQKEDIQDPSVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETQSRSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQTLLEQLS"
     gene            complement(10409..10729)
                     /locus_tag="WHT44_000010"
     CDS             complement(10409..10729)
                     /locus_tag="WHT44_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ2532257.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLKLMTNSADTRQMPS
                     ESGI"
     gene            complement(11054..11527)
                     /gene="bfr"
                     /locus_tag="WHT44_000011"
     CDS             complement(11054..11527)
                     /gene="bfr"
                     /locus_tag="WHT44_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ2532258.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(11555..12019)
                     /gene="bfr"
                     /locus_tag="WHT44_000012"
     CDS             complement(11555..12019)
                     /gene="bfr"
                     /locus_tag="WHT44_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ2532259.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            12374..12577
                     /locus_tag="WHT44_000013"
     CDS             12374..12577
                     /locus_tag="WHT44_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532260.1"
                     /translation="MPSEGLSDGMPARTDAAWSGCRFEGVFNAKGNLSYDVFAAGALK
                     KPGILGRSARLPGSISAIYSKPY"
     gene            12595..13062
                     /locus_tag="WHT44_000014"
     CDS             12595..13062
                     /locus_tag="WHT44_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HEZ2532261.1"
                     /translation="MKIENIDIISPELFPQETFNETEAFGALVWLWAVSPIYQHAGVQ
                     EAAVNILPVLKNGQFALFSSNGHPVAYCTWAYFDEETEWQYLQSNDVLRHSENWCSGN
                     RMWLINWFAPFGDSRMMKRILVHLFPKREIRWLYHRGSEKGKRIMRFPALSKQ"
     gene            complement(13103..13458)
                     /locus_tag="WHT44_000015"
                     /pseudo
     CDS             complement(13103..13458)
                     /locus_tag="WHT44_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13455..13793)
                     /locus_tag="WHT44_000016"
     CDS             complement(13455..13793)
                     /locus_tag="WHT44_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532262.1"
                     /translation="MKYIVSISLAMGLAACSFGGFKPNPWDAASFWELKNYANPYPGS
                     ASAALDQYPSKARRRQLKDMQECGYDPIDGGKSEADACLRKKGWCRKGFDPYPENKKY
                     EWPREEGKTK"
     gene            complement(13790..14152)
                     /locus_tag="WHT44_000017"
     CDS             complement(13790..14152)
                     /locus_tag="WHT44_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ2532263.1"
                     /translation="MKYIVSISLAMGLAACSFGGFKPPPDDSAFWRLTNYAKLYPGLT
                     SATLDQYPPEERRRQLHDSFAREKKDWKECGYDPIGGGGGSEADACMRKRGWYRVGND
                     IYPENKKYEWPREEGKTK"
     gene            complement(14149..20970)
                     /locus_tag="WHT44_000018"
                     /pseudo
     CDS             complement(14149..20970)
                     /locus_tag="WHT44_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714280.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
     gene            complement(21480..22463)
                     /gene="lipA"
                     /locus_tag="WHT44_000019"
     CDS             complement(21480..22463)
                     /gene="lipA"
                     /locus_tag="WHT44_000019"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ2532264.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPRNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(22456..23079)
                     /gene="lipB"
                     /locus_tag="WHT44_000020"
     CDS             complement(22456..23079)
                     /gene="lipB"
                     /locus_tag="WHT44_000020"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ2532265.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(23081..23356)
                     /locus_tag="WHT44_000021"
     CDS             complement(23081..23356)
                     /locus_tag="WHT44_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ2532266.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            23541..24611
                     /locus_tag="WHT44_000022"
     CDS             23541..24611
                     /locus_tag="WHT44_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ2532267.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMLAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            complement(24808..25755)
                     /locus_tag="WHT44_000023"
     CDS             complement(24808..25755)
                     /locus_tag="WHT44_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ2532268.1"
                     /translation="MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(25770..26177)
                     /locus_tag="WHT44_000024"
     CDS             complement(25770..26177)
                     /locus_tag="WHT44_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ2532269.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVRKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIITHP"
     gene            26258..26917
                     /gene="ung"
                     /locus_tag="WHT44_000025"
     CDS             26258..26917
                     /gene="ung"
                     /locus_tag="WHT44_000025"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ2532270.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            26914..27123
                     /locus_tag="WHT44_000026"
     CDS             26914..27123
                     /locus_tag="WHT44_000026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ2532271.1"
                     /translation="MNADIAVAAGVLKSRLICNFHLLGKTLWQSKKNQLYASLWAGCD
                     ELRGGMDASLYKELCAYAIVFKICF"
     gene            27020..27667
                     /locus_tag="WHT44_000027"
     CDS             27020..27667
                     /locus_tag="WHT44_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ2532272.1"
                     /translation="MHHFGRAATSCAAVWMRAFIKNYVLTLLFLKYVSDKHKYGGGMI
                     ELHAGTTFDDIVKLKNTADIGDRLNKIIAQIAEANDLKGVIDVTDFNDEDKLGKGKEM
                     IDRLSRLVGIFKKLNLSSNQAEDDDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSRI
                     MAKIIGISADCRPAPAFMTRPAARVRCCSKPPPKPAAKSAFTVRKKMWQPRPLPV"
     gene            27740..28213
                     /locus_tag="WHT44_000028"
     CDS             27740..28213
                     /locus_tag="WHT44_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEZ2532273.1"
                     /translation="MKTTGLRPSILPLPIRLIPPEKNGDYAFLLHLLKSLKPSGKGAI
                     ILPHGVLFRGNAEARIRTELLNLDLIKGIIGLPANLFYGTGIPACIIVIDKEHAQTAQ
                     FAEEGTNQVISGGSVFMIDASRGFIKDGNKNRLREQDIHKIIDTFTNLVTAVWCI"
     gene            28561..29505
                     /locus_tag="WHT44_000029"
     CDS             28561..29505
                     /locus_tag="WHT44_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ2532274.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEAANLTV
                     VFEETETDKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     RLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            29647..29781
                     /locus_tag="WHT44_000030"
     CDS             29647..29781
                     /locus_tag="WHT44_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HEZ2532275.1"
                     /translation="MTHLLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT
                     "
     gene            complement(30071..30586)
                     /locus_tag="WHT44_000031"
     CDS             complement(30071..30586)
                     /locus_tag="WHT44_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532276.1"
                     /translation="MKSKLLLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPVSSPSNGGQVVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILETELSNERKALVEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(31052..32974)
                     /locus_tag="WHT44_000032"
     CDS             complement(31052..32974)
                     /locus_tag="WHT44_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ2532277.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAETTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGTIDLLSGSIVRSEKLNIGYFAQHQLDTIRSDQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIVVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAVAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEEAYFEENKEKLQDTLSELAKVKTQLAQIEEVWLACQE
                     ELEQIETEIEKQFAER"
     gene            complement(33051..33446)
                     /locus_tag="WHT44_000033"
     CDS             complement(33051..33446)
                     /locus_tag="WHT44_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEZ2532278.1"
                     /translation="MMRQRGKKMLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVH
                     KDSVCLSQCRTLSDLTPENLLSDVKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGIS
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            33619..34926
                     /locus_tag="WHT44_000034"
     CDS             33619..34926
                     /locus_tag="WHT44_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ2532279.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            34919..35353
                     /locus_tag="WHT44_000035"
     CDS             34919..35353
                     /locus_tag="WHT44_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532280.1"
                     /translation="MTEPKHEMLTKEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(35412..35681)
                     /locus_tag="WHT44_000036"
     CDS             complement(35412..35681)
                     /locus_tag="WHT44_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ2532281.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(35863..38325)
                     /gene="lon"
                     /locus_tag="WHT44_000037"
     CDS             complement(35863..38325)
                     /gene="lon"
                     /locus_tag="WHT44_000037"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ2532282.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPIAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIETVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEADIKKAGMTKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGNDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     IHVPEGATPKDGPSAGIAMTLAAVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNVKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGAEAAAESASKPKPRSRATKH"
     gene            38576..39679
                     /locus_tag="WHT44_000038"
                     /pseudo
     CDS             38576..39679
                     /locus_tag="WHT44_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(39707..41452)
                     /gene="recD"
                     /locus_tag="WHT44_000039"
     CDS             complement(39707..41452)
                     /gene="recD"
                     /locus_tag="WHT44_000039"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ2532283.1"
                     /translation="MERQTDEFAQAAARAAIRFLEHYAGSGDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCRHVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSWLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEEAFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(41520..42215)
                     /gene="lolD"
                     /locus_tag="WHT44_000040"
     CDS             complement(41520..42215)
                     /gene="lolD"
                     /locus_tag="WHT44_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ2532284.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIHAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAEAAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(42208..43455)
                     /locus_tag="WHT44_000041"
     CDS             complement(42208..43455)
                     /locus_tag="WHT44_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ2532285.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDAVWVRDWTYSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPAGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            43660..43938
                     /locus_tag="WHT44_000042"
     CDS             43660..43938
                     /locus_tag="WHT44_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532286.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(43994..44611)
                     /gene="recR"
                     /locus_tag="WHT44_000043"
     CDS             complement(43994..44611)
                     /gene="recR"
                     /locus_tag="WHT44_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ2532287.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(44678..46216)
                     /locus_tag="WHT44_000044"
     CDS             complement(44678..46216)
                     /locus_tag="WHT44_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ2532288.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(46276..46650)
                     /locus_tag="WHT44_000045"
     CDS             complement(46276..46650)
                     /locus_tag="WHT44_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ2532289.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKVLSSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFNRQAPCRQG"
     gene            46740..48428
                     /locus_tag="WHT44_000046"
     CDS             46740..48428
                     /locus_tag="WHT44_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ2532290.1"
                     /translation="MLQSENSKNLISCSIKALPIMISTNGITMQFGAKPLFENVSVKF
                     GEGNRYGLIGANGSGKSTFMKILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVL
                     DVVMMGHTEMWAAMTERDAIYANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLS
                     GVGISEDLHNAKMAEVAPGFKLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVL
                     NQYDSTMIIISHDRHFLNEVCTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNA
                     KAKEKLQELQEFVARFSANKSKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEAD
                     EKAKLHRQAVEVEKLAKRFETQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAY
                     NPEYSDGLLPDEGTIKWAEKASVGYYPQDHENDFDVDMDLSEWMRQCGQEGDDEQVIR
                     GTLGRLLFGSNDVVKKVKVLSGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESL
                     NMALEKYNGTLIFVSHDRQFVSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            48500..49753
                     /locus_tag="WHT44_000047"
     CDS             48500..49753
                     /locus_tag="WHT44_000047"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ2532291.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYKFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
BASE COUNT        11234 a        12778 c        13075 g        12685 t
ORIGIN      
        1 gcaagggctt cagacggcat cttcatttcc caaatactgt ccggtaaagc gtggtataat
       61 gcgtagttat ctcttacagt ttttgaaaaa cattaattat gaaacaaatc cgcaacatcg
      121 ccatcatcgc ccacgtcgac cacggcaaaa ccacattggt cgaccaactg ctgcgccaat
      181 ccggcacatt ccgcgccaac cagcaggttg acgagcgcgt gatggacagc aacgaccttg
      241 aaaaagaacg cggcatcacc atcctcgcca aaaacaccgc catcgattac gaaggctacc
      301 acatcaatat cgtcgacacg ccgggacacg ccgacttcgg cggcgaagta gagcgcgttt
      361 tggggatggt ggactgcgtc gtcttgttgg tggacgcgca ggaaggcccg atgccgcaaa
      421 cccgtttcgt gaccaaaaaa gccttggctt tggggctgaa accgattgtc gtcatcaaca
      481 aaatcgacaa gccgtccgct cgtccgagct gggttatcga ccaaactttc gagctgttcg
      541 acaacttggg cgcgaccgac gagcagttgg atttcccgat tgtttacgct tcagggttga
      601 gcggtttcgc caaattggaa gaaaccgacg agagcaacga catgcgtccg ctgttcgata
      661 ctatcttaaa atatacgcct gcaccgagcg gcagcgcgga cgaaacgctg caactgcaaa
      721 tttcccaact cgactacgac aactacaccg gccgcctcgg tatcggtcgt atcttgaacg
      781 gacgcatcaa acccggccaa accgttgccg tcatgaacca cgatcagcaa atcgcccaag
      841 gccgcatcaa ccagcttttg ggtttcaaag gtttggaacg cgtgccgctt gaagaagccg
      901 aagccggcga catcgtgatt atttccggta tcgaagacat cggtatcggc gtaaccatca
      961 ccgacaaaga caatcccaaa ggcctaccga tgttgagcgt ggacgaaccg acgctgacga
     1021 tggactttat ggtcaacacc agcccgctgg cgggtacgga aggcaaattc gtaaccagcc
     1081 gccaaatccg cgaccgcctg caaaaagaat tgctgaccaa cgtcgccctg cgcgtggaag
     1141 ataccgccga tgccgacgtg ttccgcgtat ccgggcgcgg cgagctgcac ctgaccattt
     1201 tgctggaaaa catgcgccgc gaaggctacg aactcgccgt cggcaaaccg cgcgtcgtgt
     1261 accgcgacat cgacggtcaa aaatgcgaac cgtatgaaaa cctgaccgtg gatgtacccg
     1321 acgacaacca aggcgcggta atggaagaac tcggccgccg ccgtggcgaa ctgactaata
     1381 tggaaagcga cggcaacgga cgcacccgcc tcgaatacca tattccagcg cgcggcttga
     1441 tcggtttcca aggcgaattt atgaccctga cgcgcggggt cgggctgatg agccacgtgt
     1501 tcgacgatta cgcgcccgtc aaacccgata tgcccggccg ccacaacggc gtgctggtgt
     1561 cccaagagca gggcgaggca gtcgcttacg ccttgtggaa tctggaagac cgcggccgta
     1621 tgttcgtatc gcccaacgac aaaatctacg aaggcatgat tatcggcatc cacagtcgcg
     1681 acaacgattt ggtggtcaac ccgctcaaag gcaaaaaact taccaacatc cgtgccagcg
     1741 gtaccgacga agccgttcgc ctgaccacgc caatcaagct gacgctggaa ggtgcggttg
     1801 agtttatcga cgatgacgaa ctcgttgaaa tcacgccgca atccatccgt ctgcgcaagc
     1861 gttacttgag cgaattggaa cgccgccgcc actttaaaaa gctggattga tgtttactgg
     1921 attaatgttt aaatgatacg cgatgccgtc tgaaaaattt cagacggcat tttttattcg
     1981 gacgggcttt gcggcttctt gaagctgttt cagacggcgt ttttcctacc caatcaagaa
     2041 actgccgcca tttttccagc ggtatatcgc cccgtcgcgt ttcggtatcg ggttcggctt
     2101 cccggcagca tgtcgaacaa tgccgtttga aggaatacgc cttttgagtc cttacaggct
     2161 gaggaagagg gtaaaacata ccgcaaaaca taccacaaaa aacggtaacg gatagttgta
     2221 agcggttgat gacgattata gacaataacg ggttttccca atgaaattat tgtttgaata
     2281 ataaaaaatc ccaaaccgta aaagtttggg atttgtattt ggcagagagg aagggattcg
     2341 aaccctcgat acgctattca cgtatacacg ctttccaggc gtgcgactta aaccactcat
     2401 ccacctctct aatggcggaa attatcccaa tcgggataat ttattatttg gtgcccggga
     2461 gaagactcga acttccacac ccatgaggat accagcacct gaagctggcg cgtctaccaa
     2521 ttccgccacc cgggcaatct aaatattgaa ataaagcaaa gcatttgatt tggtgcccgg
     2581 gagaagactc gaacttccac acccgtgagg atactagcac ctgaagctag cgcgtctacc
     2641 aattccgcca cccgggcttt gctttatttg ctgttttgcg gtacagtgtc ctgccgcaaa
     2701 gaagtcgcta ttatatagtt cataaagaga atgtcaacag tccaaatgaa taaaaatatt
     2761 aaatctttaa atttacggga aaaagacccg tttttaagtc gtgaaaaaca gcgttatgaa
     2821 catcctttgc ccagtcggga atggataatc gaattgttgg agcgcaaagg tgtgccttca
     2881 aaaatcgaat cgcttgcgcg cgagctgtcg attacggaag acgagtatgt cttttttgaa
     2941 cgccgtctga aggcgatggc gcgggacggt caggttttaa tcaaccgtcg gggcgcggtt
     3001 tgcgcggcgg acaaattgga tttggtcaaa tgccgcgtcg aggcgcataa ggacggtttc
     3061 ggttttgccg tgccgctcac gcccgccaaa gacggtgatt ttgttttgta tgaacgccag
     3121 atgcgcggca ttatgcacgg cgatattgtc actgttcgtc ctgccggcat ggaccgtagg
     3181 ggccgccgcg aagggacagt tctggatatt gtcgaacgcg cgcaaagcaa agtggtcggc
     3241 cgtttctata tggatagggg cgtggcgatt ttggagccgg aagacaagcg tctgaaccaa
     3301 agcatcgtat tggaaccgga cggcgtggcg cgtttcaaac ctgaatccgg tcaggtcatc
     3361 gtcggcgaaa ttgaggttta tcctgagcaa aaccggccgg cagtggcaaa aatcatcgaa
     3421 gttttgggcg attatgccga cagcggcatg gagattgaaa ttgccgtgcg caagcatcat
     3481 ttgccgcacc aattcagtga agcgtgtgcc aaagctgcga aaaaaattcc cgtccatgta
     3541 cgcaaaagcg atttgaaagg ccgcgtcgat ttgcgcgacc tgcctttggt aacgatagac
     3601 ggcgaaacgg cgcgcgattt cgacgacgcg gtgtttgccg aaaaagtcgg acgcaattac
     3661 cgtctggtcg tggcgattgc ggatgtcagc cattatgtcc gccctgacga tgtgattgat
     3721 gcagatgctc aagaacgcag taccagcgta tatttcccgc gccgtgtgat tccgatgctg
     3781 ccggaaaacc tgtctaacgg catttgctcg ctcaatcccg atgtcgagcg tttgtgtatg
     3841 gtgtgcgata tggtcgttac ctatgcgggc aatatcaaag aataccgctt ctaccccgcc
     3901 gtaatgcgct ctcatgcccg cctgacctac aaccaagttt ggaaatggat ttcagacggc
     3961 atcgaccatc cgtacaaagc ccaaatcgac accctttaca aactcttcaa aatccttcag
     4021 aaaaagcgtt tcgaacgcgg cgcggtggag tttgaaagcg tcgaaaccca gatgattttc
     4081 gatgacaacg gcaaaatcga aaaaatcgtc cccgttgtcc gcaacgatgc ccacaagctg
     4141 attgaagaat gtatgctggc ggcgaatgtt tgcgcagcgg atttcctgtt gaaaaacaag
     4201 catacggctt tgttccgcaa ccatttgggc cccacgcccg aaaaactcgc caccctgcgc
     4261 gagcagctcg gtctgttggg gcttcaactt ggcggcggcg acaacccgtc gccgaaagac
     4321 tatgccgcgc ttgtcgaaca attcaaaggc agacctgatg ccgaattgct gcaagtcatg
     4381 atgttgcgct ccatgcagca ggcggtttac gaaccgcatt gcgacggaca ctttggtctt
     4441 gcctacgaag catacgccca cttcacctcg cccatccgcc gctatcccga cctgaccgta
     4501 caccgcgcca tcaaagccgt gttgaatcag caaacctaca cgccaaaaaa aagctggcag
     4561 gctttgggcg tgcatacctc gttctgtgag cgccgtgccg acgacgccag ccgcgacgtg
     4621 gaaaactggc tgaaaaccta ttatatgcgc gataaggtcg gcgaagtatt cgaaggtaaa
     4681 atctccggca tgaccagttt tggtatcttt gtaacactgg acggcatcca cattgacggc
     4741 ttggtgcata tcagcgattt gggcgaagac tatttcaact tccgccccga aatcatggca
     4801 atcgaaggcg aacgcagcgg catccgtttc aacatggggg acagggttgc cgtccgggtc
     4861 gcccgtgccg atttggatga cggaaaaatc gattttgtcc tgattgccgg ggggagcggc
     4921 agggggcgga aagttaaatc atccgcgtct gccaaaccgg cagggacggc ggggaaaggg
     4981 aagccgaaaa ccgccgccga gaaaaaaaca gcccgaggcg gcaaagtaag gggaaggggc
     5041 gcgtctgccg ccgcagaatc gaggaaaaag gcaaagaaac cggttccgat taaggtaaaa
     5101 aaacggaaag gcaaatcata atgctgacgg ggtggcttga ggaggcgggg catattttcc
     5161 cgaagcctcc acccgatttt ttgatgtggg cggacggagc gttatttcca gcaaattgaa
     5221 acgcccgtat tgaaagattg cgtttatttc caccgccgtt ttaaaggccg gcggtattcg
     5281 gcagacgggg cgcaaacggc gttcagacgg cattttcatt ctttcggcgt gtccgtccga
     5341 attgctttgc ccgtccgcgc aatcagcccg tgcggcttgc cttgaacgga caaaaaatgc
     5401 cgtctgaaac ccgaaaatca ggtttcagac ggcatttttc cttgaaaagg ctgttcaaat
     5461 cagcgatggt agttcggtgc ttctttggta atttgaacgt cgtgaacgtg cgattcgctc
     5521 atacctgcgg aagtgatttc cacaaattct gctttttcgt gcatttcggc aatattggcg
     5581 caacccaaat accccatgct ggagcgcagt ccgccggtca gttggtggat gatgttcaca
     5641 atcgggcctt tgtaaggaac gcggccttcg atgccttcgg ggacgtattt gtcggtgctg
     5701 tcggttttgt cttggaagta gcggtcggca gaaccttggc tcatcgcgcc caaggaaccc
     5761 ataccgcgat aggatttgta tgagcggcct tggtagagtt cgatttcgcc cggcgcttct
     5821 tccgtgcctg caaacatacc gccgagcatg acgctgtacg cgcctgcggc gagggctttg
     5881 gcgatgtcgc cggagaagcg gatgccgcca tcggcaatca gcggaacgcc cgtgcctttg
     5941 agggcttcgg caacgttgtg aatggcggtc agttgcggca cgccgacacc tgccacgata
     6001 cgggtggtgc aaatcgatcc cggaccgata ccgactttga cggcatccgc gccggcggcg
     6061 accaaatcca aagcggcttt ggcagtggcg atgttgccgc cgatgacttg gatgtgcgga
     6121 taggtttctt tgacccaacg cacgcggtcg atcacgcctt ggctgtgccc gtgggcggta
     6181 tcgacgacaa tcacgtccac gccggcctca accaaggctt tgacgcgctc ttcggtgtcg
     6241 ccgccggtgc cgactgccgc accgacgcgc agacggcctt cggagtcttt gttggcattg
     6301 ggaaactcgg tggtttttaa aatatctttg acggtaatca gacctttgag ttcgtctttt
     6361 tcgttcagaa ccaaaacgcg ctcgactttg tgcgtgtgca tcagttcgcg cgcttcgtct
     6421 atgcttgtgc cttcggggac ggtaaccaga cgttcgcgcg gggtcataat ggcggaaacg
     6481 ggcaaatcga cgcggttttc aaaacgcagg tcgcggttgg ttacgatgcc gacgactttg
     6541 ccgttttcaa cgacgggcag gccggacatt ttgcgtttgc gctgcgcacg catttccaag
     6601 acttcgcgga tgagcgttgt cggtgcaacg gttacggggt ctttgaccac gccgctttcg
     6661 tggcgtttca ctttggaaac ggcgcgcgcc tgcatttcgg gcggcatgtt tttatggatg
     6721 atgccgatgc cgccttcttg tgccatcgaa atggcgaggc gcgcctcggt aacagtgtcc
     6781 atcgcggcgg aaagcagggg gaggttgagt gtgatttcgc gggtgagctt ggtttgaagt
     6841 ttaacgtctc gcggcagcac ggtcgaatgt gcgggaacca acaaaacatc gtcgaaagta
     6901 taggcttttt ctacgatacg cataatgctc ggtctttcag tttgtgcaag atgcacggca
     6961 ttatagcacg ttaccggcgg cttgacagtt tatcaggttt aattttggtc ccctttgaat
     7021 agctcggttt tcctttgccg accactgttg ctcccgttct ttcaatttca ggaaaagctt
     7081 ttttctaatt tttggtaagt ggctcagtta ttgaagccct atatcgggcg gtaacttcca
     7141 gcacaaaaaa acggagtagt tctttattta ttttttcctt taattttcag tatattatct
     7201 taatatttcg agggtaacat atctgctaat ctagttacag ccccatatat tatagattca
     7261 attgaaaaat aacagattca actgtacctt tcttatacct gatttcttta aagtttttcc
     7321 cattgtcaaa actataaaaa agtctctctt cctcaccaga atatcccatt tctaatacta
     7381 aacaatctat ttcacaatta ccccctccac tcatatatgt agtatcaata gaaaatatat
     7441 tgcataattc cagagaatta tatttatcta tatcaaatag ttgctctcca aaatctaaac
     7501 cattgtttcc acctgcataa tacttctctt caaaggatga cttcaaacta ttaaaaactg
     7561 catttagcgt aaaatatcca tatgggcata tcttaggata tatattctct cctattatta
     7621 tctgaatttt tccataaata aaaccattac attcagagtt ttcttcccat aaaatcccaa
     7681 attttcttgt tgagtccata ataatattca tataaatcct tatattaata aattatttac
     7741 aatatccccc gctttcagac ggcatacggc gtggcggcgg aatgccgtct gaaggcgggc
     7801 gttatgataa ttgttccagc agcgtctgct tcaatgagga ctggattttt gggtttttca
     7861 ggtcggggct aaaaacggta aaactgtctt cggcgcgttc atccagtgtg gagattttgg
     7921 catagcgcag gctgacgttg tgggcgaaaa agacttccgc catatcggcg agcaggaagg
     7981 ggcggttgac ggcggtgatt tcgacggaat accagtcggg atagtcttct tcgggggtga
     8041 tggtgatgct cggtgcgatc ggcatatagc ggctgcggcg gctgatgcgg cggctgcggc
     8101 tttgggtttc ggcaacggtg tgtccgtgga taaagctgtt gagttcggct tcgagcgcgc
     8161 tttggatgtc ggggtagtct tcgggggcgt gctgcgaggg gatttgcacg atgaaggtgt
     8221 cgaggatgta gtcgtgttcg gtgatgaagg cgcgggcggc gaggatgtcg aagccgtggc
     8281 ggctgaagat gcggcagagg cgggcgaaca ggcgcgggcc gttgggcatg aaaaccatga
     8341 cttgaaagct gtcgcttttg ggcaggatgc ggctgcggac gatgggggtt tcaaagtcgt
     8401 ggactaggtt ggcggcgtgc cacaggattt cgcgggactg gtggcgggcg aagtaggcgg
     8461 aaccgagcgc gttccatagt tttttctgct gtttttcggg gacggcggcg cgggtgagta
     8521 agtcggcggc ttcctgccgg cggcggccga agagggtgtg cgggttgccg ccgttgcctg
     8581 taaggtagcg tccggcggca tggaagaggc tttccagcag gctggcgcgc catgcgttcc
     8641 acagcttggg attggtgccg cgtatgtcgg aaatggtcag aaggtagagc gcgctgaggc
     8701 gttcgtgggt ttgcacgcgt ttgcagaagg catcgagtac gctggggtct tggatgtctt
     8761 ctttttgggc gacggcagac atcagcaggt ggttttcgac cagccaggcg agcaggtcgc
     8821 tttcttctcc ggtcaggaag tggtcagcgg caaattggcg cgcgtctgcg atgccttgta
     8881 tggcatggtc gccgccgcgt cctttggcga tgtcatggaa aaaggcggca aggtagagga
     8941 tgtcttgttt ttcaaaggac tgcatcagtg cagaggcgta gggcagctcg tggctgtgca
     9001 tatccaaggc aaggcggcgg acgttgcgga cgacggtgag gatgtggtcg tccacgggat
     9061 agatgtggaa caggtcgtgt tggagcaggc cgatgatttt ttcccacgcg ggcaggtagc
     9121 ggcccaacac gccgtagagg ttgagaaagc gcagggtctg ggtcagcccg ttgccgttgc
     9181 ggaaaaaacc ggcgaagcgg cggcggtttt cgggattttg gtagaagctg cggttgattt
     9241 tgcgcgtcgc gccccaccag gcgcgcaggg tttgcggttc gagcgcggta atgtcgttgc
     9301 gctgctgcat gatttcgacg attttgaaaa tgtgttcggg ccgtctgaaa aaaatatcgg
     9361 tgtgccgcgc ggcgatttgg ttgttgactt ggatgtagtc gtcgtcaatc cgcagggtaa
     9421 cgcgcaacgg ggtggaggaa acgcggcttt gcagcatagg cgtgaggatg ccgcccagtt
     9481 gtttgacggt tttaatcgcg cggtaaaaca cgcgcatcag ttcttcgctt tggcggcgga
     9541 ggttcaagcc ttcataaccc atgctttcgg cgacttgcgg ctgcaaatcg aacagcaggc
     9601 ggtcttcggc gcgcttggcg tttaaatgca gatggatgcg gatgtgggcg aggcggcggt
     9661 agccgtgcga aagcataccg gcttcggcac gcgtcaaaat ccgctgtttg agcaggtcgg
     9721 gcaggtcggt cgccaagcct tgcgccttcg ctatccaaag cagggtgtgg atatcgcgca
     9781 gaccgcccgg acagcttttg atattcggct ccaatactgc ccccgaacct tgcgatttgg
     9841 cgtggcggtg ttccatctcc accagttttg cctcgacaaa cgccgccaca ttgcgctgcg
     9901 cgttcatttt ttccgccagt ttatccaccg tttggcggtt gccaaacaaa aacctagcct
     9961 ctaaaaacgc tgtgtccccc gtaatatcat tgcgcacgct ttcacatagt tcatcaacgc
    10021 tgccgctttt tacagacggc atcagtttgc agtcccacag ggtttgaaca aaccgggcaa
    10081 tctgttcctg aatgccgtct gaaagcgggg caggggagac aaccgccaaa tccacatccg
    10141 aacagggata cagttcgccg cgtccgaagc cgcctaccgc catcaggcat aacgcgctgt
    10201 tttgaaaata ctctgcccac aatgccgcca gtaaggtttc gactgccgcc gtgtattctc
    10261 tgaaaaatac cgacacgcgg ttggctttca aataatgcgc ttcggcggca tcgcgctgct
    10321 gtttgaaggt ttccagtgct gaagacaggt ttgcaggcat ttttattctt tcgattggcg
    10381 ggaaaaaggg aggcggatgg ttcggcggtc aaataccgct ttcagacggc atttgtcggg
    10441 tatcggcgga attggtcatc agcttcaacc gttcctgcgg cggcagcagc aactgatacg
    10501 ccgccacgcc caaagccgcc gccatttttt cgatattcga cagggcgatg ttccagcgtt
    10561 tgcgctcgac tgccgacaca taagtcctgt ccaaaccgca ttgccgcgcc aattcttctt
    10621 gcgaccaacc cttgttcacg cggaaaagcc gcatattgta tgccaatacc gcccgcaaat
    10681 cctgttcgtc aggcagttcg gcaggcagag tcaatttgtt gcccatcatt tgcttccaga
    10741 taaatggtta aagttaagca tttgctgtta cggattttac ttcacataaa agccaaaaaa
    10801 aaggggtggc ggcagccaca cccaaacaca cacacatcaa gaggaaagag ataaaatcaa
    10861 gacagattgg gaaacagcaa acaagcaccc tgaatttcat atcggtatta tcaaacaaaa
    10921 acccttatca ggtattgatt caaatcaatt tagattttat tcgcaaaccg aaaaagaaaa
    10981 acggcatatc cgtttatacg gatatgccgt ctgatggtgc gggggactgc tgtatttggc
    11041 agtggtttgt gttttagtcc tcttgcgccg ctgtttgcag gtagttcggc agaccgattt
    11101 tgccgataag ctcttgctgg gtctccagcc agtctatatg ttcttcgttg gtgtcttttt
    11161 gtttttccaa caaatcgcgg ctgacgtaat cctgttgcgc ttctgctgtg gcgatggcgg
    11221 caagcagggc ttcgtgtttt tcctgttctt tggtcaaatc gcaggcgatg atttcttcgg
    11281 tggactcgcc aatcagaagc ttgcccagtt cttgcaggtt cggcagacct tcgaggaaca
    11341 gaatgcgctc gatcaaatcg tcggcagctt tcatttcaac gatggactgt ttgaagaaat
    11401 gttcgcccag ttcttcaaag ccccagtttt tcaaaatacg ggcgtgaagg aaatattggt
    11461 tgatggttac cagcagcaag cctaagtttt tgttcagctc gcggataacc aaacggtcgc
    11521 ctttcatacg gactcctttt attccgaata cggattacag ttggctttgg tagtagttgc
    11581 cttcgccaat taactcgatc aggcgaagct gctgttccag ccagtgggcg tggtcttctt
    11641 cggtatcttt cagttgggca accatcaggt cgcgcgtaac atagtcttga gcctcttcgc
    11701 acagtttgat gccttttttc aacgcatcac gtacttcata ttcggtttgc aggtcggctt
    11761 tgaggcagga aaccacgtcc gtgccgatat tcagttcggc gcgtgccatt ttcggcgtac
    11821 cgcccagcat caggatgcgg cggatgaagt cttcggcgtg tgtggtttct tcttccatct
    11881 cgtggttgag acgttcaaaa agtttggtgt agccccattc ggagtagagg cgggagtgga
    11941 taaagtattg gtcgcgtgcc gccagctcgc cagacagcaa ttcgttcata taatcaacaa
    12001 cagcttgatt gccttgcata atatctctct ttctgtaact tggttttcgg tatgtctgaa
    12061 cagcatctgt gtcgctattc gggatgcgtg cattgtatgc aaaagctgtc ggcatgacaa
    12121 attttctatt taaaatacaa acaattatca aaagaaatag ggcgtattcc ccgatgcctt
    12181 ccaaatcagt atgctgtttc ttatcggttt ttttgctgct aaaactaaga atccatctca
    12241 tccataaaga acatttacac ttattaacca tagcaaaaaa caaatagagc acggtttttt
    12301 atcaaaattt ataatgaatc gttctcatta accgacagat tcttttgaga ttatcggatt
    12361 ttgggaataa attatgccgt ctgaggggct ttcagacggc atgcctgccc ggacagacgc
    12421 tgcttggagc ggctgtcggt ttgaaggtgt gtttaatgcg aagggcaatc tgtcgtatga
    12481 tgtgtttgcg gcaggggctt tgaagaagcc cggtatttta ggacgaagcg cgcggctgcc
    12541 ggggtcaata tccgctattt attccaaacc ttattaaaac ttaaatttaa aatcatgaag
    12601 atagaaaata tcgatattat ttcgccggaa ctgttcccgc aggaaacatt caatgaaacc
    12661 gaagcattcg gcgctttggt atggttgtgg gcagtttcgc ctatttatca gcatgccggc
    12721 gtacaagaag ccgccgtcaa tatcttgccg gtattgaaaa acgggcaatt tgccttgttc
    12781 agcagcaacg gacaccccgt cgcctactgc acttgggctt atttcgatga agaaaccgaa
    12841 tggcaatatc tccaatccaa tgatgttctg cgccactcgg aaaactggtg cagtggaaac
    12901 agaatgtggc ttatcaactg gttcgcacct ttcggcgaca gccgtatgat gaaaagaatt
    12961 ctggtgcatc tgtttccgaa acgtgaaata aggtggctgt accaccgggg gagtgaaaaa
    13021 ggaaaacgga ttatgaggtt tccagcgttg tcgaaacaat aaaacaaggc tgcctgaaag
    13081 tttcccttca ggcagccttg tttcagttct ctcgaggcca ttcgtatttt ttgttttcgg
    13141 gataaatgtc gtcacctaca cgataccaac ctctttttct catgcaggca tctgctttac
    13201 ttcctcctcc cccacctatt gggtcatagc cgcactcttt ccagtctttt tttccttagc
    13261 aaaaagatca tctatttgtt tgctatattc ttcataagaa tatatgcgta aattgatatt
    13321 tttactcatc attgccggat attcttgttc tatacgggaa tacttccagt ataccgagtc
    13381 atcgggcgga ggtttgaatc ccccaaacga acaggcagcc aatcccatag ccaaagagat
    13441 tgatacgata tatttcattt tgtttttcct tcttctcgag gccattcgta ttttttgttt
    13501 tcgggataag ggtcgaaacc cttacgacac cagccttttt tcctcaggca ggcatctgct
    13561 tcagactttc cgccgtctat tgggtcatag ccgcactctt gcatgtcttt cagttgcctt
    13621 cgtcttgctt tcgatggata ttggtcaagt gccgccgagg ctgatcccgg atagggattg
    13681 gcgtaatttt tcaattccca aaatgacgcg gcgtcccacg gatttggttt aaatccccca
    13741 aacgaacagg cagccaatcc catagccaga gagattgata cgatatattt cattttgttt
    13801 ttccttcttc tcgaggccat tcgtattttt tgttttcggg ataaatgtcg ttacctacac
    13861 gataccaacc tctttttctc atgcaggcat ctgcttcact cccaccgccc ccacctattg
    13921 ggtcatagcc gcactctttc cagtcttttt tttctctagc aaaagaatca tgcagttgtc
    13981 ttcgtctctc ttcaggtggg tattggtcaa gtgtcgctga ggttaatccc ggataaagtt
    14041 tggcgtaatt tgtcaatctc caaaatgccg agtcatcggg cggaggtttg aatcccccaa
    14101 acgaacaggc agccaatccc atagccagag agattgatac gatatatttc attttgtttt
    14161 tcctttcgga tgttgcagat cataaatgcg tacaggacgg tacataattt cgatttcgtt
    14221 ggacactcct ctttcaaata cacctgtcgg actttgggtt tgtttatttt cagggtctcg
    14281 ataaccatct gttacacatt gtttattagc cataccgtag caattatgcg gcgaggtgta
    14341 atcccctata atatccctta tagcctgcca ttggcttttt tgccgggtat tggttccgac
    14401 tgttgccgga tttctgccaa tcagcatgcc gataaggtct aattcgtggt tttctatttg
    14461 gatggactga cgggcgaaat ctgccgttct gggtgtcgtt accccctgct gcagttgaaa
    14521 cagcctttta tctgcccgga caacgttggc ggcagggccg accattttca gttcggtatt
    14581 ggataaaatt ttcttatccc gattggcttg ggtattcaag gtattgagta cattgtcgac
    14641 caccagggtt ccacggctgt gcgagccgac aaacaggccg tttttatcct tcccgtagtc
    14701 ttccatgata ttgcctaaag ccaagcctga attgctcaag ccgataacgg tcttattgcc
    14761 tatttttgcc ccttcgagca ttttatgaaa ggccgcaacg gcgatttcgg gcaattttga
    14821 aaagccccgc ccattggttt ccggattgtg caggaaataa acattttcat aggtgcgttc
    14881 atatcggttt ttctcgggat tgaaacgccc cacatattgt tgggcggcaa atttggcggc
    14941 tgcttgtata ttattgaaaa tgccgttgac gccgacaacg attttttcaa cggttttgcc
    15001 ggtctccggg tcggtgtaac gggcagtttt cagatttttc cgttcctggt cggatacttc
    15061 gcgcaattcg tagatatttc ccccaatggc tttataggct tcccaataat ctttaaatga
    15121 ggcaaattct tttttcaact ttactgagtt gtcaaactgt ttatttatat cctcttcaag
    15181 ctgcttatct tcaatcggct cgccgttttc atccatttta aaggtcatca ggcggtgttc
    15241 tgcaataaac tggctgcggt aggcttcgtc tgcaatgccg ccgatcagcg tctcattgat
    15301 aaactctttg gcaacatccc tattgagttc gacttctttc agcaagcctt cgcggtctgc
    15361 ttttgccaat gccttatgtg cggatgaagt gcctttttca ataccggcaa tatttttcct
    15421 gccctgcgcg gaagcaattt gccaatctcc ctcactgatg acggagcggg taatggattg
    15481 gcggctttct gattctttgg cattgcccaa cagattgccc aaacccattt ttgccaaggc
    15541 gtatttgtcg ttgctgtgta tatcgttttg cttcaaaccg aatttcaagc cgccggcgtt
    15601 gttttgattt aaatttgcct ctttaaaggt ttcccctttt tcaatacgct cacttcgata
    15661 tttttcgttc aactcgtcgt ctttcgccga aacctagtca aaacgcatca ggctgaccgg
    15721 tttgccgccg attttaccga tttctgcttc ttttttggtg gaatactgtc cgctggtagg
    15781 cttcgggctg tatgaaaaac cgccgctcaa gcccaaggcg gaagcagccg ccgaagcatg
    15841 gttttgaata tctttatgcc agatttcgct tgttttcagc aggttttttg atttgtcggc
    15901 atctgaaaca acagcggcgc cgaccaatcc ggttttgccg tttacgcgaa tccgatagcc
    15961 gtctcctcct gcaaagatac cgctttgctc gttgacggat gcataatccg agctgctttt
    16021 cgagcggtta tagctgccac cgacactaaa gccgtagcct accgtaactt gggcggaaac
    16081 attttcctgt ttgcctttaa acacggcggt atcctgcaaa ctttcgatat gcagactctc
    16141 tgccgttacg ccaacgcctt tgcctttaag ctgcccgcct ttgatgacgg tatcgccacc
    16201 gctttcaata gcggtttggc tgtctttgct gccgatatgg ctgttgcggt aggcggtttc
    16261 gtcgccgttg ccgtaacctt tgccgtagtt tgctccggct gtgaagccga aactgatgcc
    16321 tttgttgatg gcgatggcga ctccggcatt aaagcctgcg gatttgtttt cgctgcgttc
    16381 ctgatgcgtt tggcgggcgg cttcaatctg aacggcattt tctgctttga ggcgtgttcc
    16441 tttgccgccg tacacatcgg agccggtaat cgtgatgcgg gagtctttgc ctgcgcctga
    16501 agcagtcagg gaaactttgc cgccgccggt gattttgccc tcttgcacct gcgtgccttt
    16561 gatgcggctt tcggaggtgt tcttctgttc gccgtaggta acggagacac tgatgccctg
    16621 accggctgct tttttgggat ttcgggcggc attatagagt gccacgccgg aatctactcc
    16681 tttattcaag gcgttggcag cagccatggc attgacccgg ctgtttttgc ttttgccgac
    16741 ggtttggact gcttttacgg cgtcaaccgc gcccattacg gtattcacaa ccggaacgct
    16801 aatggcgacg gttacgcctt tttgttcgta aacctgctta ctctcttggc tgtaacggtt
    16861 ttgtgcggca tcgatgctga tttttccgga ggaaatgccg acatcgcctt ggggcgagga
    16921 tatggtcgaa ccggtttggg tgtaatgttt tcctgccgaa atcagggtat tgccgttcag
    16981 gctgccgacg acgctttctg tgtggctgac ggtctcggat cggttggttt gcgtgtcttt
    17041 tttgctgccc gccgtaaagc cgatgccgcc gctgcccatc agtccggatt tttctttttt
    17101 gttcatttcg gcactgcggc tgcgtgtttc ggctgcttta aggacgatat tgttttttgc
    17161 cgagagaatg gtatggttgt ctgcaatgat attgctgcca gtaacggtaa tatcgcgtcc
    17221 tgaaaccaga atgatttctt tgccgtccag cgtgccggat acggcttgtc cgttttggtt
    17281 cttgagatgg cgggtcatct tctgtttgat gccgcccccg cttctaccgg tgtatttcag
    17341 ggcatcttcg gtttcggtat gggctttgcc ggcttcgact ttgatatccc gtccggctgc
    17401 cagtttcaga cggccttgtt cgctgccgac ctctgctgca cggatacgga tgtctccttt
    17461 tgcattcaga ctgagattgc cccgggtgcg gatggtgctg ccgacttcgt tttgttcttt
    17521 gcgaatcaca tagttgtcgg aatcaaagat agtgttctga ttgcgggaaa tgcccgtcgt
    17581 atccgagcgg atgtcgccgc cggcattcag tacggtttga ccgtcttcag attgattggt
    17641 caattcggaa gccgtcagga cgatattgtt gcctgcatcc agcaagacgc ttccattctg
    17701 cctgccggtc agataaatgc ctgccacccg gccgatattg cgtaccgagc cttgctcatt
    17761 ctgattgctg cgggtctcgc tgcggctttc tatattgtta ctcgctttga gcagcagggc
    17821 gttttctgcg ccgatgctgc cggtattcgt aatgtcgctg cctgctgcgg cgaagatgtt
    17881 tttgccctgc aaatcacctt gcagattttt aatattctgt gcgtttaaaa ttagtgcttc
    17941 gcgcccggca attaagccgc cccggttttc aatggcgccg ctgccgatat caacaacgct
    18001 gccggacagc aacgcccctt gtccgttcat atctttgggg cgtgcgcgga catagacttt
    18061 gggtttcaat acggtttgag ttgtcccgtc gggcagggta acggtctcgt tttccagcca
    18121 aacaatgtcg gaagtcagac gggcaacctg ttcggcagac agggcaatac ccggagtaag
    18181 ctgcaattct ttggctatgg taatgccgtt atccatcaaa gccttgaatt gctcttcgtc
    18241 attggtataa ccgtccaagc ggcggtagcc tgtcagcttg gcgatttgtt cgtttaccag
    18301 tttctgctcg taatagccgt cgcccaaacg cttgtggata tggttcgggt cttgttgcag
    18361 tgcggcaagc atatagccgc tgcccagcca tttgcggtag tcggtaaagg cagggtcggt
    18421 ttcaatcaaa tagcctttgt tgtttggcgc aatggcaaac aagctgctgt tcggcagagt
    18481 aaatgtagga tggatgccgt tttcagcaac aacgggtaca acagtgccgg gtatatcaga
    18541 tgcctgttgg ggcgcatagc ctttataggc tgaaataccc atacggatgg agctgacttc
    18601 gggggccggt tcgtagggag accgactgta tcccgtagaa tcccgccctt tcttgtgatg
    18661 acggtggtaa cggtgcaagt cccctttatc ggttatggtt tttgttccca aagtttcatc
    18721 attgtccaat gcggattcgg gcgtaccgac gatcaatctg ccgccggcaa taatccggct
    18781 gttatgattt ttcagcttgg cggcatctaa aaccaaattg ccgccactga tgatttgacc
    18841 gggcttggat tcggtaattt tgctctcggc ggtgattcgt tcgtaatcgt atttgtacca
    18901 gacagagtgg gggctgccgt ccggagtccg catatgtaaa gactcatctt caaatatttc
    18961 ccagcccaac tctttttgag aaccttccgg atagcgttct gttctgcctt ccgcctgata
    19021 ttcgatacgg tgttcgcgat gggtttcttc cgtatggaaa cgcagatgct cattggtatt
    19081 ctgcaaatct tttgtagcga tacggatatt gcctgatgat tcgattgccg cactgcggtt
    19141 gtgcagtgat gtatttgctc cttgcacctg tcggcttcca ttcaatgcag aaccgatatg
    19201 aagatcgccg gagctggaca ataatgccgc ctctcggttc tcaatttccc gcgctccaat
    19261 atccaaccgc tcccgcgctg caattaccgc cgctttggtt tcgccgttga ccgtttcttc
    19321 ccggttcaac aaggtatctg cctcaactgc cacacggctg ccgtaaattc tgcccgtgcc
    19381 ggcattgtcc gacttggctt cggtttgcag cagcgttata ccgttgctgt tgattaaacc
    19441 cctgttggtg atgccgtttt tgccgtttaa cccggtgcgg tttccggatt ggattttacc
    19501 ggagacagtg ttgtcgattt gggcggcggt cagggatacg gtatcgcctg cctgtatgat
    19561 gcgggtgttt ttcagacggc cttgggtgga gaacgtgagt gtgcgtccgg cttcaatatc
    19621 gcgtttgccg gcaaaatcat catgaagaga aacggaaaca tcgcgtgcgg cggttaatat
    19681 gccgtcgttg tccagtgatt ttgcctgtaa ggaaacatct ttgcccgcaa taatcgtgcc
    19741 gtctgtgtta ttgatatgca ggctgctttt gcctgtatcg cgaatatcca gcaaaccggc
    19801 cgagccgatt aagcctgctt gattatttat gccgtctgaa accttcaata cactgccttt
    19861 gccgctgcgg ataaagcctt ggcggttatc aacggtttga gcggaaatgg tgatttcggc
    19921 agcatcaatc gaaccgctgt tgctcaattt gccgtctgcg cttaacgtaa cgccgcctgt
    19981 tgcggcaaaa atccgaccct tgttgcggat tacggcgccg ttgtcggtgc tgattaaagt
    20041 gattttgtct gcgtacatcc cacccagtgt ggcggtgtcg atggcaacgg taggagtaac
    20101 agaatccgaa gaagatggcg cagaagctgt tttggcaaga gagccgtcaa aatccaattt
    20161 gttcttaccc gaaaccacct tgacatcttt accccaaacg cccgcattga tttcagcagc
    20221 acgactaagg atacgggtgt aatcggcatc agaggtatcc aaacctttgc ccccaatcac
    20281 gactttaccc gaagaaacat caaagcccgt cagattgccg ttattcaaaa caggaacgcc
    20341 cgaagtcagc gtaaccgaag cggcattaat caatccgccg ccattcacac ggatgcccga
    20401 cggattggca acgactactt cggcgcgttt gccgccgact tcgatataac cgttcaacaa
    20461 cgaaggatta ctgctgtcaa tctggttcac aattacccgc gcttcgccgc gtgccagatg
    20521 gggattgcct tgaatccatc caccgagttg cgtttgcgta ttgctgcggc tgttgtttag
    20581 tattacgcct ttttcatcaa catcgaactg cttgaatcgg ttaacagaaa cgccttggga
    20641 tgacggagtt tgaatattga cttgcggcaa accgtttgct gtctgaagaa taacggcttg
    20701 ttggttttta ggggcggatt tgtcggcaat gatgccggaa gcaggggcag gggaaaacgc
    20761 agcaacaccc aaagccaaca tgacagaaaa ggcagccata cggaaaccga aggctgcccg
    20821 ggcagaagaa acagaagcgg caccggtcac tcgaaccgaa gccgcctcac tatcctgcat
    20881 actcttgccg tcacgatgaa cattctctgc tacagccatc atacaactgc gtttcttgtt
    20941 gaagataacc ttgtagcatc gcttgttcat gatgggtttt cttaaatgaa atgtaaggaa
    21001 tagttaagga caaaatatag gaaatttgaa ttaaattgtc aataaaaaac tgacagcgta
    21061 ccctgataat cagatgttgt acgctaattg tgagaagtat gtcgggatga ttattttgaa
    21121 gttttctttt tattcaaaag agttactatg aaagttaacc gggcgtaaat aggcttcaat
    21181 aaacaggtta tcgttatttt tcaattggga aaaaatcttc cctaacccat ccattcgcca
    21241 aattattatg tgggaattcg gcaatttcaa taacggctgt tgatccatat ctgctcggtc
    21301 atttagcgtt ataaagtcgc aaatagcgtc agaatttaca agattatcgg attttgggaa
    21361 taaattatgc cgtctgaagg gctttcagac ggcataggcg gctaacacgg gtgcggcttg
    21421 cgccaaacgg cgcggcaggc gcagggaatc gctttttact ttggcgcggt gttccgggtt
    21481 taatgatgcc cgcaaccgcc gtggctttcc ctcaaggctt ccgccgcctg ttcgtcggcg
    21541 tggtagctgg aacgtaccat cgcgccgatg gcggcattgc tgaagcccag ttcgtatgct
    21601 tctttttcaa agattttgaa ctgctcgggc gtaacgtagc gcaggacggg caggtgtccg
    21661 tctgaaggct ggaggtactg tccgatggta atcatttcga tattgtgcgc ccgcatatcg
    21721 cgcataattt cacgcacgtc ttcgtctgtt tcgcccaagc cgaccatgat gccggatttg
    21781 gtcgggatgt gcggcatcat ttctttataa cgttttaata agtctaaaga atgttgataa
    21841 ttggcaccgg gacgggcttt tctgtacagg ctcggatggg tttctaggtt gtggttcatc
    21901 acgtcgggcg gggtttcggc aaggattttg agtgcgatgt ccaagcgtcc tcggaagtcg
    21961 gggacgagga tttcgatttt ggtgttcggg ctggtttcgc ggatggcttt gatgcagtcg
    22021 gcgaaatgct gtgcgccgcc gtcgcgcagg tcgtcgcggt cgacggaggt gatgacgacg
    22081 taacgcaggt tcatggcttt gacggattcg gcgaggtttc tcggttcgtc ggggtcgagc
    22141 atattgggcc gaccgtgtcc cacgtcgcag aacgggcagc ggcgggtgca gatgtcaccc
    22201 ataatcatga aggtcgccgt gcctttgctg aagcattcgc cgatgttggg gcaggaggct
    22261 tcctcgcaaa cggtgtgcat cttttgttcg cgcaaaatgt ctttgatttc aaagaatttg
    22321 cgcgatggga gtttggcgcg tatccattcg ggctttttca gtttttcctg aagggggacg
    22381 actttgatgg ggatgcgcgc ggttttgtcc gcgcctctga gtttgatgcc gcgtttgggg
    22441 tcgtcggttt tgatttcact cattgttgtc tgctttcggt gtgaattgtg tttcaaggtg
    22501 tgcggtgagt ttggcggcga cttcgtccgg cgtggggcag ggttggacaa aatccgcgat
    22561 ttgcgtcatt tccataccgg cgtagccgca ggggttgatg tgggtaaacg ggcttaaatc
    22621 catattgacg ttgagcgcaa gcccgtgata gacggagccg tttttgatac gcagccccag
    22681 tgaggcgatt ttgcgttctc cgacataaac gccggggcgt ttggggtctg ccgccgcttc
    22741 gatgccgtat tctgccaatg tggcgatgat gctgttttca agcgcggaaa cgatgtttct
    22801 aacactggtt ttgcgccgtt tgaaatcaat catcgtataa acgaccaatt gcccgggccc
    22861 gtgataggta atctgcccgc cccggtcgat ttggacgacg ggaatgtcgt cgcgaatcag
    22921 caggtgttcg ggttttcccg ccagtccttg tgtgaacacg ggcgggtgtt cgacgaccca
    22981 cagttcgtct tcggtgtcgg cattccgtcc ggcattaaag gttttcatcg cttcaaaagt
    23041 cggcagatat tcgaccaaac ctttgtgtat gattttcatc tcaaagtacc actttgacca
    23101 gttcgtgcga agtcagcgca cggtagatgt tgtccaattg ttcttggttt tcaaccttta
    23161 cctgtacggt ggcgccagta tagttgcctt tgctgctcgg acgcgtggtg atgtggtgcg
    23221 cctgcgtgtc gggggcgtgg aggcggacgg tgtctaaaac cgcctgctcg aactcgggat
    23281 gcaccgcgcc cattactttc aatgggaagg tgcagggaaa ttcgatgagg gatgttttgt
    23341 ttttttgttc ggtcatgatg tgctgccttg tcgtgtacgg tatgccgtct gaaggcgggt
    23401 ttgcctttca gacggcatcg gatgtgcgtt attttagcct aaaccgcgat aacaggctat
    23461 cgggaaggcg gaggcttttt tgacggcgcg gcggttctgc tatactggcg cacaatatta
    23521 tttttagaag ggtggttttt atgtatcgga ggaaagggcg gggcatcaag ccgtggatgg
    23581 gtgccggtgc ggcgtttgcc gccttggtct ggctggtttt cgcgctcggc gatactttga
    23641 ctccgtttgc ggttgcggcg gtgctggcgt atgtattgga ccctttggtc gaatggttgc
    23701 agaaaaaggg tttgaaccgt gcatccgctt cgatgtctgt gatggtgttt tccttgattt
    23761 tgttgttggc attattgttg attatcgtcc ctatgctggt cgggcagttc aacaatttgg
    23821 catcgcgcct gccccaatta atcggtttta tgcagaacac gctgctgccg tggttgaaaa
    23881 atacaatcgg cggatatgtg gaaatcgatc aggcatctat tattgcgtgg cttcaggcgc
    23941 atacgggaga gttgagcaac gcgcttaagg cgtggtttcc cgttttgatg aggcagggcg
    24001 gcaatattgt cagcagtatc ggcaacctgc tgctgcttcc cttgctgctt tactatttcc
    24061 tgctggattg gcagcggtgg tcgtgcggca ttgccaaact ggttccgagg cgttttgccg
    24121 gtgcttatac gcgcattaca ggcaatttga acgaggtatt gggcgaattt ttgcgcgggc
    24181 agcttctggt aatgctgatt atgggcttgg tttacggttt gggattggtg ctggtcgggc
    24241 tggattcggg gtttgccatc ggtatgcttg ccggtatttt ggtgtttgtc ccttatctcg
    24301 gggcgtttac gggattgctg cttgccaccg tcgccgcctt gctccagttc ggttcgtgga
    24361 acggcatcct atcggtttgg gcggtttttg ccgtaggaca gtttctcgaa agttttttca
    24421 ttacgccgaa aatcgtggga gaccgtatcg ggctgtcgcc gttttgggtt atcttttcgc
    24481 tgatggcgtt cgggcagctg atgggctttg tcggaatgtt ggcgggattg cctttggccg
    24541 ccgtaacctt ggtcttgctt cgcgagggcg tgcagaaata ttttgccggc agtttttacc
    24601 ggggcaggta ggcggttccg aaacatatag tggattaaca aaaatcagga caaggcgacg
    24661 aagccgcaga cagtacaaat agggcaacgc cgtactggtt tttgttaatc cactatattt
    24721 gaagcggaat acaaccttgc ccgggtttaa ataaaaatgc cgtctgaacc ccgaaaactg
    24781 gacttcagac ggcattttca tcacggctta tttggcggtt ttgctgctgt cgataatttt
    24841 cataccggca gaaatcaggc tgccgatgtc ggcaacattg gcgggcataa tcagcgtatt
    24901 gctttctttg gcaagattgt tgaacgcagc gacgtattgt tccgcaatct tcagattgac
    24961 cgcatccgca ccgccttggg tttgaagggc ggcggcaatt tgacggatgg cttcggcatt
    25021 ggcttcggca acaaggcgca aggattccgc ttcacctttg gcgcggttga tgcgggcgat
    25081 tttctcggca tttgacgcat tgaccgcagc ctgagcctcg ccttcggatt gttggatttc
    25141 ggcttcgcgc tgaccactgg caaggttgat ttgttcgatt ttacgacctt cggattcggc
    25201 gatacgggcg cgtttttcgc gttcggcagt aatttgcgcc tgcattgagc gaaggatttc
    25261 ttgcggcgga accaagtctt taatctcata acgcaaaacc ttcacacccc aagccccggc
    25321 cgcctcgtcc aaagccgcaa caacagtact gttgatttcg tcgcgttctt caaacgtttt
    25381 gtccaactcc atacgcccga taacggaacg cagcgtcgtt tgggcaagct gggtaatcgc
    25441 cataatgtag ttgctcgaac cgtatgaggc gagtttgggg tcggttactt ggaaatagat
    25501 gatgccgtca acagtcagct gcgtattgtc gcgcgtgatg cagacctggc tgggtacgtc
    25561 taaagggatt tctttcagcg aatggcggta ggcgacgcgg tcgataaagg gaatcaaaat
    25621 attcaaaccg gccgtcaggg cgcgatggaa acgccccagc ctttcgacaa cgtggacttc
    25681 ctgttgtggg atgacaacaa aggatttgaa accgaaaacg gcgacggcta ccaacaagat
    25741 aatgaaaaat tccataattc ctccgagtgt taagggtgtg tgataataag aaggttgcct
    25801 tccttgcgga caatgagggc gcgagttcct ggttcaagct cttcttgccc cgtattttga
    25861 gcctgccagt gcgtaccgcg ataaaaaact tcgtaacggt tgccgcctgt gtgtcggagg
    25921 atttcgacat attgtccggc atccaaatcc tgatatgaat ccgtttcaac ttttctaacg
    25981 gcggttttgg cgtgtacgaa ccaaataccc agcgcggaaa gcagagcggc ggtcaagacg
    26041 gcggcaggcg tactgccggt cagcccgtaa gcaatgcccg aacccgccaa agccgcgctg
    26101 acaaccaaaa gataaaccgt tcccgtcaat aattcgatga ttaagacggc aacagcggca
    26161 acaaaccata cagtcataca tttccccaca aagcgcgtcg tttgacaaaa taacgcaata
    26221 tcagcagtat agccgaattt gaaaggatag ggcagatatg gacacttggc acgatgcact
    26281 cggcggcgaa aaacagcagc cgtattttca ggaaatttta aatgcagtca ggcaggaacg
    26341 tttgtcggga caaatcatct atccgccggc ggcggatgtg ttcaacgcat tccgcctgac
    26401 agcgttcgac cgggtcaaag ccgtcattct cggacaagat ccgtatcacg gggcagggca
    26461 ggcgcacggt ttggcatttt ccgtccggca gggtatccgc ataccgccgt ctttactcaa
    26521 tatctacaag gagttggaaa ccgacatcga aggcttttcc attcccgcgc acggctgtct
    26581 gacagcgtgg gcggagcagg gcgtattgct tctgaacacg gttttgacgg tgcgtgcagg
    26641 acaggcgcat tcgcacgccc ttttaggctg ggaacgcttt accgataccg ttatcaggca
    26701 gcttgcgaca caccgcaagc accttgtctt catgttgtgg ggtgggtatg cacaacaaaa
    26761 agggaggctg atagacagtc aaaatcattt gatattgacc gcaccgcatc cgtctcctct
    26821 gtcggcatat cgcggttttt tcggctgccg ccatttttca caggcaaaca gctatttgag
    26881 ccggcacggt atcgatccga taaactggaa gctgtgaatg ccgatatagc cgttgccgcc
    26941 ggcgtgttaa aatcgcgttt gatttgtaat ttccatttat taggcaaaac cttatggcaa
    27001 tcaaaaaaaa atcagcttta tgcatcactt tgggcgggct gcgacgagtt gcgcggcggt
    27061 atggatgcga gcctttataa agaactatgt gcttacgcta ttgtttttaa aatatgtttc
    27121 tgataagcat aagtacggcg gcggcatgat tgagctgcac gccggtacga cttttgacga
    27181 catcgtcaaa ctcaaaaaca ccgccgacat cggcgaccgc ctgaataaga ttatcgccca
    27241 aattgccgaa gccaacgact taaaaggcgt gatcgacgtt accgacttca acgacgaaga
    27301 caaactgggt aaaggtaagg agatgatcga ccgtttgagc aggcttgtcg gcatttttaa
    27361 aaagctcaac ctttcttcca accaagccga agacgacgat ttgttaggtg atgcctacga
    27421 atacctgatg cgccattttg cgaccgagtc aggcaaatcc aaagggcagt tttacacgcc
    27481 tgccgaagtc tcccgcatta tggcgaagat tatcggaatc agcgcagatt gccgtccagc
    27541 accagcgttt atgacccgac ctgcggctcg ggttcgctgt tgctcaaagc cgccgcccaa
    27601 gccggcagcc aaatcagcct ttacggtcag gaaaaagatg tggcaaccgc gtcccttgcc
    27661 cgtatgaata tgattttgca caacaacgaa accgccgaaa tcaacaccgg gaacaccttg
    27721 tccgattcgt ctttccgtga tgaaaacgac gggcttaaga ccttcgattt tgccgttgcc
    27781 aatccgcctt atcccgcccg aaaaaaacgg cgattacgcc tttttgctgc atctgctcaa
    27841 aagcctgaaa ccaagcggca aaggtgcgat tattcttccg cacggtgtgc tgtttcgcgg
    27901 caatgccgaa gcgcgtattc gcacggaatt gcttaacctt gaccttatta aaggcattat
    27961 cgggctgcct gccaacctgt tttacggcac gggcattcct gcctgcatca tcgtcatcga
    28021 caaagaacac gcccaaaccg cccaatttgc cgaagaggga acaaaccaag ttatcagcgg
    28081 cggcagcgtg tttatgattg acgcatcgcg cggcttcatt aaagacggca acaaaaaccg
    28141 tctgcgtgag caagacattc acaaaatcat cgacactttc acaaacctcg ttacagccgt
    28201 atggtgcatt taagcgaaat cgcagcacaa gattacaacc ttaatctgcc tcgctatatc
    28261 gacagcggag aagtcgaaga cctgcaaaat ctcgccgcct atctatcttt atggcggcat
    28321 acctgcgcac gatatggacg cattggaagc ctattggcaa gttttaggcc gtatgaaaaa
    28381 cgagttgttt gccgaacacg atggccactt taccactata caacggaatc gattgcaaat
    28441 ctttcccact ctcaacagct taaaatcctg cgggattggt gtggaattta gggctaatct
    28501 agtacagccc caaatttaat ccactataaa atcgaaagca gccaaatcaa agcccatata
    28561 ttggcgcacc ccgattacgc cgccttcaaa gccggacacc tagcaaagtt tgccgcgtgg
    28621 cacactcaaa acgaccttgc cgccatccaa ccgggcaggc ttatccggaa atggagcgaa
    28681 agcctgctgg acgcgttcaa acccggcagc ctgattgaag aatacgattt ctaccaaatc
    28741 ctgacggact actgggcgga aaccctgcaa gacgatgttt atctcatcgc ccaagatggc
    28801 tggaaggcgg ttaaaaacct ggccgaaatc accaaagaaa gcgatgaagc cgcgaacctg
    28861 accgtcgtct ttgaggaaac cgaaaccgac aaaaaaggca aagccaaaac caagcgcatc
    28921 agcaaaaaat accgcagcga agtcatcgcc cccgagctgg ttgcccgccg ctacttttca
    28981 gacggcatcg ccaagctgga agaaaaacaa agcgagctgg aacgcctaag ccaagaattg
    29041 gaaaaccaca tagaagaaca cggcggcgaa gagggtgcgc tgaacgacgt attggatgca
    29101 aaaggcaaac tttccgccaa acttctgaaa accgcattgg aagaaagcgg catagaagaa
    29161 ggcgaacggg ctgttttaca aaccacccaa acactgatga cgcaggaaaa agccgcgaaa
    29221 gacgcagtca aaacccaaat cgaagccctg aaccttgccg tattcaaaca atttggccga
    29281 ctttccgaag ccgaaatcaa gcagcttgcc gttcaagaca aatggcttgc cgatttacaa
    29341 agccgaatcg aaaatcgctt ggaaaacagt attcagcagc ttatcagccg cttgaacacg
    29401 ctggaagacc gctaccgcag cccgatggcc gagcttgccc gagaagtgga aaagtggcaa
    29461 agcaaagtca atgcccacct tgaaaatatg ggttttggag gctgaaatgg cagcacagac
    29521 aggctataag gcgagcgggt tttgagacct ttgcaaaatt ccccaaaatc ccctaaattc
    29581 ccaccaagac atttagggga tcgcggttcg ggtgtccgca ccgcttaata cgtcgtcgtc
    29641 cacgaactga cccatttgct cgaacgccat cgcaacgccc gttttatggc gcatatggac
    29701 aactttctcc caaactggca aagcatcaaa caacagctta atgccttgga gttatttgca
    29761 caaatatata atttaacata atatacatta tgcgaactat cggaaacagt tgtacgtgtc
    29821 cctgtggtct ttccaagtag gaaaattaaa gtatggccaa tgcggctgaa tgtatagccc
    29881 ggagcatccg cgtatccgaa ggtactgaac gacatgccct gcatcccttt cgacgcttcc
    29941 atccagccgg tattgggaaa tacgttgacc ttcaatataa tatacaggca gccgacacac
    30001 aggatatgcc gctgcttttt catcatttct tctgtcaaat ccttggaacg gtcgatttga
    30061 aaacacggct ttacatacgc cccagttccc tttgcagggc ttgaatattt tgctgcctgt
    30121 ccaatacatt gctttgtaat gcatttattt cttgatggtt gatgttgccg ccctttgcca
    30181 gacgtgcttg tgataacatt ttttgggctt caaccaatgc tttgcgttcg ttgctcaatt
    30241 ctgtttcgag aatggagcgt ctgctgttgt ttgagggtgc ttgttgcggc ggcggcgtat
    30301 tggattttgc cggcttggat actgttttga ccggggcttt atatttgaca acctgtccgc
    30361 cgtttgacgg tgatgatacc ggttcgggcg tttggggcgg gatatagcgt tcgctgctgt
    30421 agttgccgat tgggggcaaa tcggttgagt ggcagctttt ggacggcttg gtggtgtaaa
    30481 cggtttctcc gttgattgtg caggtgtaga ttttggccgc attcgcaccc aatgggcttg
    30541 aaatcaggga aaagttgatt aggattaaga ggagttttga tttcataatg tgcttgattt
    30601 tcggataatc atttgatttt ttggtatttt ttgtaatgct ttcttggcgt tttacgggct
    30661 tttcttttcg gttggttgct tttgattttc tttcggggct ttggcagcct gcttgttctt
    30721 tttcccggca ggttttttgt ttttcttttt actttctatg tgtattttgg cttcttaact
    30781 gagtttttta attttcaggc ggtatccgcc tccctgatgg ctgctgattt taggtaaatc
    30841 cgcatcggcg cacaatcctg ctggggctga tgtatatacg cttttgccat ttgagttgca
    30901 atggtatacg gaggcttgcg ccgccgcgcc ggaaagggac aggagaccca aggcggcaaa
    30961 aagtctgatg tttataccgg taaaaggcgg tgataacgcc cgaattatac cgttattggc
    31021 aggcaaagat aagcaccctg cccgcgcttc tttatcgctc ggcaaactgt ttctcgattt
    31081 cagtttcaat ctgttccaat tcttcttggc aagccagcca aacctcttcg atttgggcaa
    31141 gttgtgtttt gacttttgcc agctcggata aggtgtcctg caatttttct ttgttttcct
    31201 cgaagtaagc ttcttcttgt gctaaaaatg cttcacatgc cgtctgaatt tcggaaagct
    31261 gcgccatttc tttttcggca cggtctattt tctgctgtat cggcttgccg cgtcgggctt
    31321 tttcctgacg gatttgcgct tcgatgcgct tggtgtcttt gcggctttgg ctttgtgcgg
    31381 atgctgcggg cgcgacggcg gcgttttcct gtgccaaacg ccattggcgg tagtcgttca
    31441 aatcgccgtc gaagttcttc agacggcctt tatcgatcag gaggaagctg tcggtcgtgg
    31501 cttcaagcag gctgcgatcg tgcgatacga cgattaaggc gccttggaaa ctttgcagag
    31561 cgagcgtcaa ggcgtggcgc atatccaaat ccaaatggtt ggtcggctcg tcaagcagca
    31621 gcaggttcgg cttttgccag ataatcatgg caagagcgag tcgggctttt tctccgccgg
    31681 aaaatggttc ggttttctgc aacgccatat cgccgacaaa attgaagcct ccgaggaaat
    31741 ttcggatttc ttgttcgcgt acttcgggag aaagctgctg aatatgccaa acagggtttt
    31801 ggtcggagcg gatggtatcg agttggtgtt gggcaaaata gccgatattg agtttttcgg
    31861 aacggacgat gctgccggag agtaaatcga ttgtgcctgc caaagctttg ataaaggtag
    31921 atttaccgct gccgttgaca cccaataaac catagcgcgc gccgctttcc agcgacaggg
    31981 taatgtcgtg caaaacagtt ttgccttcgt aacccaaatc tgcgtgttct agctttaaca
    32041 aaggattggg cagatggtcg ggatggtaaa actcaaagga aaactcgctg tccagatgcg
    32101 cgggagcgat gcgttcgagc ttcgccaaag ccttcatgcg gctttgcgct tgaacggctt
    32161 tggtggcttt ggctttgaag cggtcgataa aggattgcaa atgtttgatt tgcgcctgct
    32221 gtttgacata ggcagcttgt tgttgcgcga gacgctgcgc acgttcgttt tggtaaaaat
    32281 cgtaattgcc gccgtattgc gtgagttttt gctgcgataa ttcaatggtt tgggtagttt
    32341 ccgcgttgag aaaatcgcgg tcatgggaaa tgatgatttg cgtgcagggt aaagaagcaa
    32401 ggtggttttc cagccacaag acggtttcca aatccaagtg gttggtcggt tcgtcaagca
    32461 agagcaaatc ggcgcggcaa atcagggctt gcgcaagatt caggcgcata cgccagccgc
    32521 cggaaaagga tttgacgggg cggctgtgtt cttcttgcga aaaacccagc ccgttcaaca
    32581 attttgccgc acgcgccggc gcggtataag cgtcgatttc ttccaattta gcatgatatt
    32641 ccgcctgctt catgccgtca ttttgcgctt ctgcctgcct caatgccgtc tgaaaagcct
    32701 gcaactcggc atcgccctgc aaaacgtaat ccaaagcgga aatatccaaa tcgggcgttt
    32761 cttgggaaac ggaagcgagc cgccagtttt tcggaatcga gacatcgccg ccgtcctgag
    32821 tgatttcacc cttgattaag gcaaacaggc tcgatttgcc cgttccgttt ttgccgatca
    32881 aaccgacgcg ctgaccggga ttgacggtag cgttggcttt gtcgagcagg actttcaaac
    32941 cgcgttgcag ggtgaggttt ttgatttcaa tcataacgga aacatcgtcg ggcgggaaaa
    33001 gcccgtattt tacctgaaag tcagtgccga tgccgtctga aacgggaaat ttacggctga
    33061 agccaagccc aagccctgcg cccttccgag tgcaggaaaa ccaatgtcct gaatgccgaa
    33121 tcggtattca tgcattccac gctgattccg attcgggaaa aatccgccat gattttggga
    33181 tggataaact cctgagccgc gcccgtcccg ataatcaata tttccggata gtcaacaggt
    33241 ttgacgtcgg acaacaggtt ttccggagtc agatcggaca aggttcggca ttgcgacagg
    33301 cagaccgaat ccttatgtac aagcacgggt ttatggaaac tttgccccgc cagccggatt
    33361 ccgcccgcac cgcattcata ttccgcaaac tgtccgtcta tcggattttc ttcaaacaac
    33421 atttttttac cccgttgccg catcatctac accgaaaggg atgcaaaatc agacaaattc
    33481 atgtaggatt ggcagatttc atctgacccg cctgccgatt tcagacggca tttgattcaa
    33541 agtgcggcac aattatatcg gcagcggata ttttcgtctt tcaatattta catttcagtc
    33601 ggcttacaag gagacacaat gaagccagta aacatcggtc ttttaggttt gggtacggtc
    33661 ggcggcggta cggctgccgt gttgcgggac aacgcggagg aaatttcccg tcgcttgggg
    33721 cgcgaaatcc gtatttctgc cgtgtgcgat ttgagtgaag aaaaagcccg acaaacctgc
    33781 ccgtccgcag cctttgtcaa agatccgttc gaactggtcg cacgtgaaga cgtcgatgtc
    33841 gtcgtcgaat tgttcggcgg taccggcatt gccaaagatg cggtgttgaa agccattgaa
    33901 aacggcaaac acatcgttac cgccaacaaa aaactgctcg ccgaatacgg caacgaaatc
    33961 ttcccgctgg cggaaaaaca aaacgtcatc gtccaatttg aagcggcagt agcgggcggt
    34021 atcccaatca tcaaagccct gcgcgaaggt ttggcggcaa acaggattaa atccatcgcc
    34081 ggcattatta acggcaccag caacttcatc ctctccgaaa tgcgcgaaaa aggcagcgcg
    34141 tttgccgatg tactgaaaga agcgcaggca ttgggttatg ccgaagccga tccgaccttc
    34201 gacatcgaag gcaacgatgc gggccataaa atcaccatca tgagcgcact ggcattcggc
    34261 acgccgatga acttttccgc ctgctacctc gaaggcatca gcaaactcga cagccgcgac
    34321 atcaaatacg ccgaagaact tggctatcgc atcaaactgt tgggcattac ccgcaaaacc
    34381 ggcaaaggca tcgagctgcg cgtccaccct accctgattc ccgaaagccg cctcttggca
    34441 aacgtcaacg gcgtgatgaa cgccgtgcgc gtcaacgccg atatggttgg cgaaacctta
    34501 tattacggcg cgggcgcggg cgcattgccg accgcttccg ccgtggttgc cgatatcatc
    34561 gacatcgccc gcctggttga agccgatacc gcccaccgcg taccgcatct ggcgttccaa
    34621 cccgcgcaag tccaagcgca aaccatcctg cctatggacg aaattaccag cagctactac
    34681 ctgcgcgtcc aagccaaaga cgaaccgggc acgctggggc aaatcgccgc gctgttggca
    34741 caagaaaacg tgtccatcga agcactgatt caaaaaggcg tgattgatca gaccactgcc
    34801 gaaatcgtga ttctgaccca cagcacggtc gaaaaacaca tcaagtcggc aatcgcagcc
    34861 atcgaagcac tggattgcgt ggaaaaaccg attaccatga tccgcatgga aagcctgcat
    34921 gactgagccg aaacacgaaa tgctgacgaa agagcaggtt gccgcgcgca aaaaagcaaa
    34981 agccaaaatc cgcaccatcc gcatttgggc gtgggtcatt ttggcgttgc tcgctttaac
    35041 cgccctgctc tcccaatgcg cgatgtccaa accgcaggca aaacagaaaa ttgtcgagtc
    35101 ttgcgtgaag aatattccgt ttgccgaaaa atggcaaaac gatttgcggg cccgcggttt
    35161 agattcaaac aatacccgcc tcgccgtcga ctactgcaaa tgtatgtggg agcagccttt
    35221 ggacagattg agcgagaaac agattagatc cttcggcaaa ctcggcgcac aagaacagct
    35281 tgacctgctc ggcggcgcaa atgcctttga agcacgtgac aagcagtgtg ttgccgattt
    35341 gaaatcagaa taatgtggac cgataaaaaa gccgattctt taaagaatcg gcttttttca
    35401 taaaaaacgg cttacagtgc gtctttcaaa gctttgccgg cgcggaattt aggcgttttg
    35461 gcggcggcaa tggtcagagg ctcgccggtt ttggggttgc ggccttggcg ttccgcacgt
    35521 tcgcccacgt agaaagtacc gaaaccgacc aaagtaacgg tgtcgccttg tttcagggcg
    35581 gtggttactg cattggtagt ggcatccaaa gctttttgtg cggcggcttt ggaaatgtcg
    35641 gcttcttgag caatcgcttc gatcaattca gacttgttca caatcagtcc cttcctgtct
    35701 taaaaaatga tgaaatgccc gaatactcgg ggtttgtact gcttgagcaa ctttcgcttt
    35761 atagcaattc tgaaattgcc gtgtcaagca aaaaatacgg aatcacccta tttgacaggc
    35821 tttcaggacg aaaccgcatt tttacaacac atttcctgcg tttcaatgtt tggttgccct
    35881 gctgcggggt tttggttttg aagcggattc cgccgccgct tccgcaccag aaggttctgc
    35941 ccaaggctca ggctggcttt ccaaacccag agccaatacc tcgtctatcc atttgaccgg
    36001 atggatggtc aggccggttt tcacgttttc agggatttct tccaagtctt tgacgttgtc
    36061 tttcggaatc aggacgtgtt tgatgccgcc gcgcaaggcg gccaacagtt tttccttcaa
    36121 accgccgatg ggcaaaactt cgccgcgcag ggtaatttcg cccgtcatcg ccacatcggc
    36181 gcgtaccggg attttggtaa aggcagatac cgccgccaag gtcatcgcaa tacccgcact
    36241 agggccgtct ttcggcgtcg cgccttcggg aacgtggatg tggatgtctt ttttctcgta
    36301 aaaatcagga gccaaaccca ctgattccgc acgggagcgg acaaccgacc acgctgcgga
    36361 cacggattcc ttcatcacat cgcccaactg gccggtgcac tgaatcacgc ccttacccgg
    36421 caatgctgcg gcttcgacgg tcagcaattc gccgccgact tccgtccacg ccaaaccggt
    36481 aacctgcccg atacggtttt cgctttcggc aacgccgtaa tcgaagcggc gcacacccaa
    36541 atagtcgtgc agatttttct catttacttt aaccgcttta ggtttggctt tgctggtttt
    36601 cttggtttca gacaacctct tcttatcttc gtccaaggta atctgcatca ccaccttgcg
    36661 gcagattttg gcaatttcgc ggtcgagcga acgcacgccc gcctctcggg tgtaataacg
    36721 gataatatcg cgcaccgcgc tttcttcgat tgccaattcc ccttctttta caccgttgcg
    36781 cttcatttgc ttcggtacga ggtactgcat cgcgatattg attttttcgt cttcggtata
    36841 gccggacaga cggatgattt ccatacggtc gagcaacgga gtcggaatat tcagactatt
    36901 ggatgtggcg ataaacatca catcactcaa atcgtaatcc acttccgcat aatgatcggc
    36961 aaacttgttg ttttgttcgg gatcgagcac ttcgagcaac gcgctggcgg gatcgcctcg
    37021 gaagtcgtta cccaatttgt cgatttcgtc gagcaggaac aaggggtttt tcacgccggc
    37081 ttttgccata ttctgcaaaa tcttaccggg catagagccg atataggtgc ggcggtgtcc
    37141 cctgatttcg ctttcgtcgc gcacgccgcc caaagccatg cggacatatt tccgccccgt
    37201 tgctttggcg atggattcgc ccaaagaggt tttgcccacg cccggagggc cgaccaggca
    37261 cagaatcggg cctttgagtt tgtccatacg tttttggacg gcgaggtatt ccaaaatccg
    37321 ttctttgact ttttccaggc cgtagtggtc ggcatccagc accagtccgg ctttggcgat
    37381 gtctttgctg acgcgggatt ttttcttcca cggcagctcg agcaaagtgt cgatgtagtt
    37441 gcgtacgacg gtggattccg cagacatcgg tggcatcatt ttgagctttt tcagttcgga
    37501 caggcatttt tcttccgctt ctttggtcat acccgccttt ttgatatctg cttccaaggc
    37561 atccagttcg ccgttttcgt cttcttcgcc cagttctttg tgtatcgctt taatctgttc
    37621 gttcagataa tattcgcgct gggatttttc catttggcgt ttgacgcgtc cgcgtatgcg
    37681 tttttcggcc tgcataatgt cgagttcgga ttccagctgt gccagcagga attccatccg
    37741 tttgccgatt tcgggaattt ccaaaatctg ttggcgttgc gccagtttca actgcaaatg
    37801 cgctgcgacc gtatcggtta gccggctgtt ttcggcaatg ccgttgatgc tgccgataat
    37861 ttcggcgggg atttttttat tgagtttggc gtattgttca aactgcgcca acagggtgcg
    37921 gcgcacggct tcgaggtcgg tattgccgcc cgtgtcttct tccacgaccg tctctatatg
    37981 ggaaacgaac agaccgcccg tgtcttcaat ggtcagaaca cgtccgcgat acagcccttc
    38041 gaccaatact tttaccgtgc cgtcgggtag tttcaacact tgcaggactt gtgcgaccgt
    38101 accggtctga tacaggtcgg cggcaatcgg ttcttctacc gccgcatcgg tttgcgccaa
    38161 caggaaaacc ggctcctcgc gggtaatggc gttttccagt gcggcgatgg atttcggtct
    38221 gccgacaaac agcggcagaa ccatatgcgg gtaaacgacg acatcccgca aaggaagggt
    38281 tgccaaggcg gcatattcct caaaatgctt ttctttttgt gtcataggta ctctcttgtg
    38341 tctgacagat tgccgatttt cgcgtacatt ggggttgaag gtattatttc aagcatatgt
    38401 ggtttattta tggagtttga tgcgatgccg tctgaaacat tccggcttca gacggcatgg
    38461 gcttggaaag acaaggcggg aacaaaaaac tgttctgtgt tgccgctcct tgctgtacca
    38521 tccgtatggt ttgcggttct gccgccctat tttcaaaaca tgacgcaggt atcccatgtc
    38581 ttcttttatt tttcccgatg acgggttcga atggcgcaac gaaagtctgc aaaagccgcc
    38641 caaaggttgg cattatgtcc gcgaaagcgg tgcagacggc attttgaagg ctgcctatca
    38701 aaatattgca actgtctagc agggcgattt ccacaatgcc aaacaggtgc tttctgcaat
    38761 gaagaagagg gttcgcaaac ctgccgccgt ccgttccgat gcggatatcg ccgccctttt
    38821 ccatgcccac cgtatgaagc aggcgcagca gagccgtatt ctgaatatgc ttgccgttga
    38881 aatccgcccc ggttttgtgt tggacaacaa acgcgcgccc gatatacgct ccgctttgct
    38941 cgacgtgtac ggagaggcgg acggcaaacc gtttttcctg ccgctcaatc tgctgctggg
    39001 gtttatgggt gcgcacgagt ggcataagaa aggggttgcc gttccgcagc tgggcggcag
    39061 catacacgtt cctttcggcg tattctcgcc gttgcgcggc gaatacctcg acctgctcgc
    39121 ccatgcgccg tcaacgggtt ttcagacggc attcgatatc gggacaggct ccggcgtgct
    39181 tgccgccatt ttggcgaaac agggcattcc ttccgtcatc ggcacggata ccaatccgaa
    39241 agccgtcgcc tgcgcccgtg ccaatattgc ccgtttgggc tttgaaaaac aggttgagat
    39301 acgggaaacc gatctgtttc ccgaagggtt tgccgatctg attgtctgca atccgccctg
    39361 gcttcccgcc aagccgactt ccgccgtcga atccgcgtta tacgaccccg aatctgcgat
    39421 gctggctgcg tttttgcggg atgcgccgaa acatctgaat cccgacggag aaatccgcct
    39481 gatcatttcc gatcttgccg aacatctgca cctgcgtcca tccgattttc tggataaggc
    39541 atttgctcag gcgggtttgc gtgttgccga tatgatgaaa accaagccga agcacaaaaa
    39601 agccgcgaat ccgagcgatc cgcttgcttt tgcgcgaacc cgggaaacca ctttcctata
    39661 ccgtttgaaa aaggcataag gggcggcggc gcggcattcg ggcggattat tcttgtgaaa
    39721 atacccgctc gagcatactg cccaatgccg tctgacgcgt tttgacggtg gcggcagctt
    39781 gccggaaggc ttcttccccg ccgaagaata cgaacttctc tctcgcgcgg gtaatggcgg
    39841 tatataacag ctccttactc aatccggaca atgcatcgtc cccttcgtcc gaaggtgcgg
    39901 cggaaggcgg cagcagccat acttcccggt attccgaacc ttggcttttg tggacggtca
    39961 tggcgaatgc gggttcaaat tcgggcagcc agcttaccgc taccttttta aatccgtccg
    40021 catcggcaaa ataggcggca aggctgccct gccgtccgac atcttccata atcagtccga
    40081 tgtcgccgtt gaacagttca agcgcgtagt cgttctgcct gatcataatc ggctctccgg
    40141 caaaatatgc caaatgttcc ggtatgttca ttttgcggcg tacatggcgg caataggctt
    40201 cgttgaagtc ttccgcatcc tgccgccaag ctgccagaac cacgatatcc gaaatgcccg
    40261 cgtatgcggc ttcgatattg ccgtctttta ccgcctgcca ataggctttg tgtgcccggt
    40321 acaacctttc gactcgagcg ttcggactgc attccgaatg ttccagttcg tccggaaacc
    40381 ggtcaaacaa tgcccacgcc ccttcatcgc ccgatacggc ggcacgggca aggcagccga
    40441 tgccgctgtt gtcgccgaag cggtggctga acgacagatg ggcggtgttt tgcgccaaca
    40501 cgggtggatt tgcgctgacg ctgaaaccgt gttccggaag gaagccggcc agcctttggt
    40561 gcgtttctcc gtccaaaacg gttttttgtg acaaaacgga cagcaccgcc cctattccga
    40621 cggacgggag ctggttttca tcccccagca gaatcacgcg cgcgccggtt ttgaccgctt
    40681 ttaaaagttg cagcatcaat gccgtatcca gcatagaggc ttcatcgata accaatacgt
    40741 caaacggcag cgggtaaaca gggttgaacg ccgcctgcat tttgggcggg cgcagcttca
    40801 gcagtcggtg gacggtttgc ccttccagtt tgagcaaatg gcggcggacg gcctccggcg
    40861 cgtcaaaacc gttgattgca cggtgcagcg cgcgcgccat atgtgccgcc gctttgcccg
    40921 tcggtgcggc aagcgcgatg ttgggaagat tttcgtcttc accgcaaatc agcgccagca
    40981 gtttggcaac cgttgtcgtt ttgcccgttc ccggcccgcc ggtaatcacc ataaaagact
    41041 gcaacagtgc caaggcggcg gcatcgcgct gcccttcgct gcccgtgcct tgaaaccatt
    41101 ttgcgaggtt ttgcctcgcg cctgccgcgt cgggggcgga tgtgccggct gccgccaagc
    41161 gttttatctc ggcagccaaa tcgtattcca actgccacat cctgcccaaa aacagccttc
    41221 tgccttccaa aatcaaaggc gcggcggatg ttccgacaac gggtgcgagt gccgacagcg
    41281 cgtcagcctc gccaccgctc aaacggataa acgaatgacc gttttgcaat gcctgaaaca
    41341 ggcgttcggt gcagtttgca agcacttcgt cgccggaacc cgcatagtgt tccaaaaaac
    41401 ggattgccgc ccttgccgcc gcttgggcaa attcatctgt ctgccgttcc attttattcc
    41461 ttatccaaat gccgtctgaa ggcgtggggc ttcagacggc gcggtgttgt ttcggctgtt
    41521 taggcgtttg cgccctgttt ggggtgcagg ctgccgtctt tcatgaccat cacgcgctcg
    41581 aagcggccgg cgagttcgtc gtcgtgcgtt acgaccacca gccccgttcc caattctgtt
    41641 ttcagttcca gcatcatatc caaaacattc ctggcgttcg cacgatcgag attgccggtc
    41701 ggttcgtcgg caagcaggca tttgggttgg gtaaccaagg cgcgtgcaat ggcggcacgc
    41761 tgccgctcac cgcctgaaag ttcgcccgcg cggtgcgtcg aacggtgttt cagtccgacc
    41821 ttttccagca tcgccattgc cgcctccgca gcctcttcac ggcttttttt gccgatcaga
    41881 agcggcatca tcacattttc cagtgccgaa aattcaggca gaagatggtg gaactggtac
    41941 acgaaaccga gatggcggtt acgtaaatcg cccaaacgcc gctggtttaa ggtacgcaaa
    42001 tcctcgccca tcagcagcac cctgccttca gacggcatat ccagcccgcc caaaatatgc
    42061 agcagcgtcg atttgccgct gcccgaagaa ccgatgatgc cggtgctttc ccctgcgtgg
    42121 atttccaaat ccaagccgtg cagcacccga acgtccaaac cgccgtcccg atagcgtttg
    42181 cccacgcctt cgcatttcaa aatcaactca ctcataacgc aaagcctccg ccggttgggt
    42241 ttttgacgcg cgccggctcg ggtagagcgt ggcaacgaaa gacagtccca aagaaatgca
    42301 ggcaatcagg gcaacgtcgc ccatatcgac atcgctgggc aggtagtcga taaaataaac
    42361 ctgcgaattg atgaggtgga caccgagcag gttttcaaaa aacgccacga ccctgccgac
    42421 gttccaaccc aaaagcacgc cgcagaccac acccgccagc gtgccgaaaa agcctgaaaa
    42481 cgcgccctgc accataaaaa tcttcatcac gccagcaggg gaaagaccca aagtccgcaa
    42541 aatcgcaatg tccgcctgct tttccgtaac cgccatcacc agggaagaga caaggttgaa
    42601 cgccgccaca gcgataatca gcgtcaggat gatgaacatc atccgttttt ccagttcgac
    42661 cgcttcaaaa tagctgcggt tgctgtacgt ccaatcgcgc acccaaaccg cgtccctttg
    42721 cgcctccgga atcagtgttg ccgtcaaggc gggagcgttt tgcggatcgg cgagcttcag
    42781 ccgcagcccc gcaacttcct tatccaaacg gtacagcacg cgcgcgtctt ggatatgcgt
    42841 cattgccaat gagttgtcca cttcgtaaac gcccgtctta accagaccga ccacggtaaa
    42901 ctgtttcaac ctcggtacga ctccggcggg cgtaacattg ccctccggcg tgatgacggt
    42961 aactttattg ccgacttccg cccccaaagc ctccgccaag ccgacaccga ggataatgtc
    43021 aaactcgccc ggaatcagat cttcaaattt gcctgccggc attttgtcgc cgtattccac
    43081 cactttgcgt tcttcagacg gcaaaatgcc gcgcatctgc acgcccctga tttcgcccgc
    43141 attggccagc aatgcctgat tggaaacata gggcgcggca gccaaaatac ctttgcggtt
    43201 ttcggtaaac cgaagcaggt tgcgccaatc cgtatccgta ttatcgatat agccgatttc
    43261 ggcgtgcggc gcgacattca ggagctgccc gcgtatttct ttctgaaagc cgttcataac
    43321 cgacaagacg acaatcagcg cggttacgcc caaggcgatt ccggcaatcg aaaccatcgt
    43381 gataaacgac ataaagccgt tgcgcttttt cgccctgaga tacctcaagc ctatccaagc
    43441 ctctagagaa aacataacgc taccttaaaa atgtctgcaa acgtgccgcc ccggacggcg
    43501 gtttgggggc ggcggcaaaa gtttattgta ccgtaaaacc caggcagcgt ccgaacgccc
    43561 gagtgcgggg gacggggcgg cagagcgggc ggaaaacttg cacaacgcgt caaactgccc
    43621 tatcctttcc ttcagaaaaa ccgtttcttg aggaaaacaa tgaatatccg aactgctttt
    43681 gctttgtgcg ccatcgcctt atccgccgct gcggctgcct acgccaaaga aatcaaaatc
    43741 gatgccaaca acacgcctta ttccgaagcc gacgcgcaaa agctggcggc aacggcagtc
    43801 ggtatgggtg ttaaggaacc tatcagcctg aacggcggca gcggcagcat taccgtgtcc
    43861 ggcagcagcg cgacgcagtg cgtgttcaaa gtcggcaacg gaggcgcatt gcagattcaa
    43921 gggctaaact gcaagtaaac cgcccggaaa aatgccgtct gaaggcttca gacggcattt
    43981 tgcattggcg gcgttatgcc ccgccttctt taatcaggcg gcgttcgtac accgcctgcg
    44041 ccagcgttcc cgcatcgaca tattccaatt cgccgcccaa gggaatgccc tgcgacagcc
    44101 tgctgacttt gtaaggcagg tttttaaaaa actcggacag gacatacgcc gtcgcattgc
    44161 cttctgcggt aaaagcggtt gcaataatga tttcttcgac ttccccgccg cccagccgtt
    44221 gcgccagcct gtccaatgcg atggcggata cgtccattcc caatgccgta ttgatttgcc
    44281 ccatcaggac gaaatacagc ccgtcgtggc agtttgccgc ttccatattc gacacgtcgg
    44341 caggcatatg caccaccatc agccgccgcc cgtcgcgtgt ttcatcggca caaatatcgc
    44401 acaatccgcc ttcgcaaaac gtgttgcaca tcgcgcaatg gtaaacctgc ttcaatgccg
    44461 tctgcaaggc atccaccagt ttttcagcct ctttgcgctt gtgttggagc aaatgatacg
    44521 ctatccgctg tgccgatttc ggcccgacgt tgggtaaaac cttcagcgcg tcgatcaatc
    44581 cttggaaggc atcttgtttt ttgtggctca tcatattccg ccgtatggga aaacggccgg
    44641 aatattccga ccgttatttt gtcaacaaaa gtgtcaatta ctgaccgtcg ccgttgtcga
    44701 ccgattgcgc tcctttggtc tgtttgattt tgccgttgaa ataacgtatc aacaagtcga
    44761 aagtattggc agactgctgt tgcgccaaag cctgttttgc aagcggaagc tgtgcggcga
    44821 tatcatccgg cggggttaca gcctgtactt cgacaatcac gggtgccggc agaccgatca
    44881 gcctgacgta ggcgggtttg ccgtttgccg gttttgcttt cagcagttcc gcataagcct
    44941 cgggcggcat ggactgcctt gcctgctgtg cgcccaaaac ggacacttcc gaccatttca
    45001 cgtcaacagc cttgccgccg ttcagttggg taagcacgtc ttttgccttg ttttcggcaa
    45061 gtttggcggc ttcggtacgg atataagcct gacgtaccgc gtctttggct tcggcaaacg
    45121 gcagggtttt ctcttcgcgg acttctttgg cgcggacgac ccacgcggtt tcgctgttga
    45181 tggtcagcac ttcggaattg tgttttttct tcaatacgtc gtcgctgaat acggcattga
    45241 tcaggttttc gggcataccg gacatttgcg cgtcctgcct actcagccaa gtttcttggg
    45301 tttcgacttt caaaccgctg tttttggcgg cttcggcaag cgaggaagga tggttgaacg
    45361 catcgtcgcc caatttttct tttgccttgt tgaagtcggc aaccgccttt ttcattttca
    45421 attcgttttc gacggcggct ttttcctgct cgaaagaagg tttggcttca tttgccggca
    45481 aacgcgccac gcgctcttca aatgcatttt tcacttccgt ttcactgacg gtctgcttgt
    45541 ctgcaaaatc cttcagattc aaggcgacat attccaattt gaccgcctgc ggcagcagat
    45601 agtctttttt gttcgcatta taaaatttct gcaaatcggc ttcagacact ttgacttggg
    45661 cgatgaactc gtcggggttg aaagtgtgcg aacggatggt gcggttgacc tgtgtcagcc
    45721 tgatcagctg ttccgcctgc gcgtcgccga ccaatacgcc gttttggacg aggtttacca
    45781 aattctgcaa ggcaaactga tcgcggattt cttcgacaaa ctggtcttca gacatatggc
    45841 gttgggaaag gtagcggttt aaaagcgcgt ggtcgaattt gccgtttgcg tcgtggaaat
    45901 tgggatcgtc cacgataatt tgcttgattt gttcggaaga aaccgaaatg cccatcagct
    45961 tcgcgccctg tttcaggtag gcgcgttgca gcagggattg gaacaccgcg tcgcgcgaag
    46021 ggccgccgcc gtccgcctgt tcgttctgta tggcgttgtt gatggagtgg tcgctgattt
    46081 tttcgtcgcc cacttggacg atgtagtcgg cacccggatg ggataccgtg ctgaccccga
    46141 agccgacgaa ggttaatgca atcaggccca aaaggacttg ggcgggcgtt ctgtattttt
    46201 cgatggaatg gaacatattt taaatcggga tatagaatgg gaacgggaaa ttcaagtcgg
    46261 gtattgtaac ggtttttatc cctgtctgca cggggcttgc cggttgaaga tgccgtcgta
    46321 ggtttcttcg ctgaagccgg cgtaaacctt gccgccgcac tccaatacgg gacgcttgat
    46381 caggctcggc atttcggaca tcagtttgac ggcctccgcc gtcgaggaca gcactttttg
    46441 ctgtgtttcg gcatcgagtt tgcgccagct tgtcccgcgt ttgttgagca gggttgccaa
    46501 aggcacttgt tccagccacg agcagatttc cgcttcagac ggcctctgtt ttttaaaatc
    46561 ccgaaattca aactccaagc cgtatccggc aagccggttt ttggcttttt tgaccgtatc
    46621 gcaatttggg atgccgtgaa ggactatcat ttggaaacct tttgtctgaa ataataaaac
    46681 ggatatttta ctataagtgt ctgaaaattt gcccgtctgt ttcagacggc ggggcggtta
    46741 tgttacaatc cgaaaattcg aaaaatttaa tctcttgttc aataaaggct ttaccaatca
    46801 tgatttctac caacggcatc accatgcagt tcggcgcaaa gccgctgttt gaaaacgtat
    46861 ccgtcaaatt cggcgaaggc aaccgttacg gcttgatcgg cgccaacggc tcaggcaaat
    46921 ccaccttcat gaaaatcctc ggcggcgatt tggaacagac ggccggcgaa gtggcgattg
    46981 aaaacggcgt gcgtttgggt aaattgcgcc aagaccagtt tgcctacgaa gacatgcgcg
    47041 tgctggacgt ggtgatgatg gggcataccg aaatgtgggc ggcgatgacc gaacgtgatg
    47101 cgatttacgc caatcccgaa gccaccgaag acgactacat gaaagccgcc gaactggaag
    47161 ccaagttcgc cgaatacgac ggctacaccg ccgaagcgcg tgccgccgaa ctgttgagcg
    47221 gcgtgggcat ttccgaagat ttgcacaatg cgaaaatggc ggaagtcgcc ccgggcttca
    47281 aactgcgcgt attgctggcg caggcgctgt tctccaagcc ggatgtattg ctcttggacg
    47341 aaccgaccaa taacttggac attaatacca tccgctggtt ggaaggcgtg ttgaaccaat
    47401 acgactccac gatgattatc atcagccacg accgccactt tttgaacgaa gtctgcacgc
    47461 atatggcgga tttggactac aacaccatca ccatctatcc gggcaactac gacgactaca
    47521 tgctcgcctc cgcccaatcg cgcgaacgcg ccctgaaaga caatgccaaa gccaaagaga
    47581 aactgcaaga gctgcaagag ttcgtcgccc gcttctctgc caacaaatcc aaagcccgtc
    47641 aggcaaccag ccgtctgaaa caggccgaca aaatcaaatc ggagatggtc gaagtcaaac
    47701 cttccacccg tcaaaacccg tatatccgtt ttgaagccga tgaaaaagcc aagctgcacc
    47761 gtcaggctgt ggaagttgaa aaactggcga aacgctttga aacccagttg tttaaaaacc
    47821 tgaacttcat ccttgaagcg ggacaacgcc tcgccatcat cggcccgaac ggcgcgggca
    47881 aatccaccct gctgaaactc ttggccggcg cgtacaaccc cgaatattca gacggcctgt
    47941 tgccggacga aggcaccatc aaatgggcgg aaaaagccag tgtcggctac tatccgcaag
    48001 accatgaaaa cgacttcgac gtcgatatgg acctgagcga atggatgcgc caatgtgggc
    48061 aggaaggcga cgacgaacaa gtcatccgcg gcactttggg gcgtttgctc ttcggcagta
    48121 acgatgtcgt gaaaaaagtg aaggttctct ccggtggtga aaaaggccgt atgctttacg
    48181 gcaaactgtt gctgttgaaa cccaatgtct tagtcatgga cgaaccgacc aaccatatgg
    48241 acatggaaag catcgaatcc ttgaacatgg cactggaaaa atacaacggc acgctgattt
    48301 ttgtctccca cgaccgtcag ttcgtttcct ccttggcaac ccaaatcatc gaactggacg
    48361 gcaaaggcgg atatgaacac tacttgggcg attacgaaag ttacttggag aaaaaaggcg
    48421 tagcataacc gccggttgga acaatgccgt ctgaagccgc ttcagacggc attgttgata
    48481 actttaaaat aggaagcata tgcagactta tctcgtcggc ggtgccgtcc gcgattatct
    48541 tttgggcttg cccgtcaaag accgcgattg ggtggtcgtc ggcgcagacg cacaaaccat
    48601 gctggcgcaa ggcttccagc cggtcggcaa agattttccc gtgtttctcc atcccgaaac
    48661 acacgaagaa tacgccctcg cccgcaccga gcgcaaaacc gccaaaggtt acgtcggttt
    48721 cagtttccac gccgacaaag acgttacgct ggagcaggat ttgatgcgcc gcgacctgac
    48781 catcaacgcg atggcgcaag atgcggacgg caagattatc gaccctttcg gcggacaacg
    48841 ggatttggcg gcaggcattt tgcgccacgt ttccccagcc tttgccgaag accccgtccg
    48901 catcctgcgt actgcccgct ttgccgcgcg ttacaagttt gaaatcgccg aagaaaccat
    48961 aaagctgatg cggcagatgg tggaaaacgg cgaagcggac gcattggttg ccgaacgcgt
    49021 ctggcaggag tttgcgaaag gtttgatgga aaaaaatccg cgcaaaatga ttgaagtgtt
    49081 gcgcgaatgc ggcgcgctca aagtcttgct gcccgaagtc aatgccctct tcggcgtgcc
    49141 gcaacgcgcc gactaccatc ccgaaatcga cagcggcatc cataccctga tgacgctgca
    49201 acgcgccgcc gatatgggct tgagcctgcc cgaacgctat gccgccctgc tgcacgactt
    49261 gggcaaagcc aaaacaccgt ccgacatcct gccgcgccac cacggacacg acctcgccgg
    49321 tgtcgaaccc gtgcgcgaag tcaatcagcg gctgcgtgcg ccgaaacatt gcgccgagct
    49381 tgccgaattg gtttgccgtt ggcacattat tttccaccaa gtcggacagc ttaaaagcca
    49441 aaccattctg aacgttttga aaaaaaccga cgctttcaga cgacccgaac gctttcagac
    49501 ggcattgaac gtctgcattg ccgacacgca aggccgtctg aaccgcgaac acacgcccta
    49561 cccgcaacgc gcgcactggc tcgccttact cgaagccgcc aatcaggcgg attcgggcaa
    49621 aatcgccgcc gaatgccgcg cacagggaaa agcgcacctt atcgccgaac aaatcgaccg
    49681 ggcgcggctg gcacaaatcg ccccattgca aaaagcgttt cgagcggcgc aagacaaaac
    49741 agaaaaacat taaaacgtcc aatgcagcca ct
//
LOCUS       DAWGRZ010000002        39302 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000002 DAWGRZ010000000
VERSION     DAWGRZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.002"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(49..714)
                     /locus_tag="WHT44_000048"
     CDS             complement(49..714)
                     /locus_tag="WHT44_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ2532292.1"
                     /translation="MTVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFIVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARSDSPFMLES
                     LKRRGVTEPDAVAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALANMMTGAAMA
                     GIDSCPVEGFNYAEMERILSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(824..1720)
                     /locus_tag="WHT44_000049"
     CDS             complement(824..1720)
                     /locus_tag="WHT44_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ2532293.1"
                     /translation="MQLLKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTIARLTMTVYLPAEQKGTHMS
                     RFVALMEQHAEALDFAQLRKLTTEMVALLDSRAGKISVSFPFFRKKTAPVSGIRSLLD
                     YDVCLTGEIKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            1789..2946
                     /locus_tag="WHT44_000050"
     CDS             1789..2946
                     /locus_tag="WHT44_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ2532294.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKVLAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPLDCWLVLRGIKTLALRMNAHCQNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYELAKTQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHDVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(3009..4010)
                     /gene="hemB"
                     /locus_tag="WHT44_000051"
     CDS             complement(3009..4010)
                     /gene="hemB"
                     /locus_tag="WHT44_000051"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ2532295.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGDKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(4193..4477)
                     /gene="yhbY"
                     /locus_tag="WHT44_000052"
     CDS             complement(4193..4477)
                     /gene="yhbY"
                     /locus_tag="WHT44_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ2532296.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            4587..5207
                     /locus_tag="WHT44_000053"
     CDS             4587..5207
                     /locus_tag="WHT44_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ2532297.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFEGLLNNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            5271..7238
                     /gene="ftsH"
                     /locus_tag="WHT44_000054"
     CDS             5271..7238
                     /gene="ftsH"
                     /locus_tag="WHT44_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ2532298.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            7460..7891
                     /locus_tag="WHT44_000055"
     CDS             7460..7891
                     /locus_tag="WHT44_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ2532299.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSTTLVEAFVKEADRRYA"
     gene            7884..8162
                     /locus_tag="WHT44_000056"
     CDS             7884..8162
                     /locus_tag="WHT44_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ2532300.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            8215..8793
                     /gene="pth"
                     /locus_tag="WHT44_000057"
     CDS             8215..8793
                     /gene="pth"
                     /locus_tag="WHT44_000057"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ2532301.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARATLPDGDVWLLKPTTFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQARLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSAEHR
                     QQIDDAIAKSLQAIPDIISGKWEEATRFLHSK"
     gene            8853..9059
                     /locus_tag="WHT44_000058"
     CDS             8853..9059
                     /locus_tag="WHT44_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ2532302.1"
                     /translation="MSELNRLIQTESVAVIEETLNFLLYECSIDDAPSAEEIAVWRDM
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            9056..9418
                     /locus_tag="WHT44_000059"
     CDS             9056..9418
                     /locus_tag="WHT44_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ2532303.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            9649..11064
                     /locus_tag="WHT44_000060"
     CDS             9649..11064
                     /locus_tag="WHT44_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEZ2532304.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            11183..11692
                     /locus_tag="WHT44_000061"
     CDS             11183..11692
                     /locus_tag="WHT44_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ2532305.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            11855..13237
                     /locus_tag="WHT44_000062"
     CDS             11855..13237
                     /locus_tag="WHT44_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ2532306.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIDVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIIEKDGFVAADKQGNVTEKDISGEYHNHI
                     VGHIKLKRPMKIAIDAGNGVGGAFAGKLYKGLGNEVTELFCEVDGNFPNHHPDPSKPK
                     NLQDLIVELKNGEAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLAPWIKEHGGEPVMEKTGHSFIKSAMKKTGAPVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(13257..14012)
                     /locus_tag="WHT44_000063"
     CDS             complement(13257..14012)
                     /locus_tag="WHT44_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ2532307.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(14022..14738)
                     /locus_tag="WHT44_000064"
     CDS             complement(14022..14738)
                     /locus_tag="WHT44_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ2532308.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(14728..15555)
                     /locus_tag="WHT44_000065"
     CDS             complement(14728..15555)
                     /locus_tag="WHT44_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ2532309.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVVRNDSNIKSIADIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            15840..16748
                     /locus_tag="WHT44_000066"
     CDS             15840..16748
                     /locus_tag="WHT44_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ2532310.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALTA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            16828..20442
                     /gene="recB"
                     /locus_tag="WHT44_000067"
     CDS             16828..20442
                     /gene="recB"
                     /locus_tag="WHT44_000067"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ2532311.1"
                     /translation="MSASIQAFDPLTVPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRGRLDDVLQVLESKGIAKLGDDTLSDGIAAYCAE
                     HHEGDTFLPELLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVEMTEEDGDRLLIPAQDFWRERVSNDPVLAALALKRKAVPQTVLAQISRYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTICRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHADT
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAARGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSSIFGYDAQQLHDFNQNESEILHWAESARTALDNWNKYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLGDQISLAENNSGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQGANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGSPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRVADNAPSGTNFAFTENAPPPAAYRSNAGQTAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYDLSDISPECRCPEMGFTLHTEDFSLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFVDMVCQDPDGNICVIDYKSNHLGTDASAYTQQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
     gene            20481..20840
                     /locus_tag="WHT44_000068"
     CDS             20481..20840
                     /locus_tag="WHT44_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ2532312.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            21082..21561
                     /locus_tag="WHT44_000069"
     CDS             21082..21561
                     /locus_tag="WHT44_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ2532313.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPSTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(21837..23216)
                     /gene="radA"
                     /locus_tag="WHT44_000070"
     CDS             complement(21837..23216)
                     /gene="radA"
                     /locus_tag="WHT44_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ2532314.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTDGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(23402..24466)
                     /locus_tag="WHT44_000071"
     CDS             complement(23402..24466)
                     /locus_tag="WHT44_000071"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ2532315.1"
                     /translation="MISLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEADIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(24620..25060)
                     /locus_tag="WHT44_000072"
     CDS             complement(24620..25060)
                     /locus_tag="WHT44_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532316.1"
                     /translation="MGSSDSFKEKKEIFEIGTPAYRQKLIDVWKKSINGNEKSWVLFE
                     NGTCVILLEPEKDLAKQAKEMLSKWGKVQIGTPSADFGIITLDSGDGYAVSCHHPEIF
                     TLILKEEGLDEDFKIGIEGRSHRDCDAEEPKVIHIEDKRTIETP"
     gene            complement(25093..26316)
                     /locus_tag="WHT44_000073"
     CDS             complement(25093..26316)
                     /locus_tag="WHT44_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ2532317.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVLNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDEIGEPQKAEAQRNLVLE
                     AVSDDERHAALEQHS"
     gene            complement(26313..27647)
                     /locus_tag="WHT44_000074"
     CDS             complement(26313..27647)
                     /locus_tag="WHT44_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ2532318.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLMLDGVLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NEKTASEPAAASDVKTIEAPSLPSERKPEQPAKKQTVPEKAGRSPSAKGERA"
     gene            complement(27662..28423)
                     /locus_tag="WHT44_000075"
     CDS             complement(27662..28423)
                     /locus_tag="WHT44_000075"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ2532319.1"
                     /translation="METAKPVMLIVRPSGRAAEDVETCLNAGWRAEVLSPVEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKAQIAVGQGSRRALERCLVRTV
                     IAPDDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            complement(28444..28863)
                     /locus_tag="WHT44_000076"
     CDS             complement(28444..28863)
                     /locus_tag="WHT44_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532320.1"
                     /translation="MKKMFLSAVLLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRWATMKFQNGEFMYTIWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFS"
     gene            complement(29114..29434)
                     /locus_tag="WHT44_000077"
     CDS             complement(29114..29434)
                     /locus_tag="WHT44_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ2532321.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLGKEAAEQGLIWFAEHVADARAHPGKHPNIDLLENVVQSGETWLLKP
                     LSAQ"
     gene            complement(29457..30083)
                     /gene="upp"
                     /locus_tag="WHT44_000078"
     CDS             complement(29457..30083)
                     /gene="upp"
                     /locus_tag="WHT44_000078"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ2532322.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLATELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPISYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(30189..30500)
                     /gene="grxD"
                     /locus_tag="WHT44_000079"
     CDS             complement(30189..30500)
                     /gene="grxD"
                     /locus_tag="WHT44_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ2532323.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(30601..30816)
                     /locus_tag="WHT44_000080"
     CDS             complement(30601..30816)
                     /locus_tag="WHT44_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEZ2532324.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREAKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQY"
     gene            complement(30842..31621)
                     /locus_tag="WHT44_000081"
     CDS             complement(30842..31621)
                     /locus_tag="WHT44_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ2532325.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVEAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRTV"
     gene            complement(31626..31976)
                     /locus_tag="WHT44_000082"
     CDS             complement(31626..31976)
                     /locus_tag="WHT44_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ2532326.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            complement(31980..32957)
                     /locus_tag="WHT44_000083"
     CDS             complement(31980..32957)
                     /locus_tag="WHT44_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ2532327.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TVSGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            complement(32991..34103)
                     /locus_tag="WHT44_000084"
     CDS             complement(32991..34103)
                     /locus_tag="WHT44_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ2532328.1"
                     /translation="MTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRT
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            34289..34990
                     /locus_tag="WHT44_000085"
     CDS             34289..34990
                     /locus_tag="WHT44_000085"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ2532329.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGRFSAYEGELAGK
                     RMVLALSGIGKVNAAVATAWIIREFAADCVINTGSAGGLGKGLKVGDVVIGTETAHHD
                     VDVTAFGYAWGQVPQLPARFASDGILIEAAKRAARTFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AANSAKMVAEIVKSL"
     gene            35134..36927
                     /gene="lepA"
                     /locus_tag="WHT44_000086"
     CDS             35134..36927
                     /gene="lepA"
                     /locus_tag="WHT44_000086"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ2532330.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVIYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            37070..38089
                     /gene="lepB"
                     /locus_tag="WHT44_000087"
     CDS             37070..38089
                     /gene="lepB"
                     /locus_tag="WHT44_000087"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ2532331.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPDGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRS
                     GTAIR"
     gene            complement(38245..39150)
                     /locus_tag="WHT44_000088"
     CDS             complement(38245..39150)
                     /locus_tag="WHT44_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ2532332.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFRQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFA
                     SGLVSGLMLALSMVFVRKTHEIEPVALFPSMMILSLGGAVSLVVPALLMDGGALYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKRQSSH"
BASE COUNT         9425 a        11143 c         9935 g         8799 t
ORIGIN      
        1 attaaaaaat gccgtctgaa aatccttgcg ttttcagacg gcattgcctt atgcccaaat
       61 aacggtttct tccaagggcc tacgcgcttt cgtggcgatt tcctgaacgc ggtagccgaa
      121 tgtcgcggcg acggacacgc cccattctgc cgcatcgaac aaaccaaact gcccggacaa
      181 tatgcgctcc atctcggcat agttgaaacc ttccaccggg caggaatcga tacctgccat
      241 cgccgcaccc gtcatcatgt tggctaacgc gatataggtc tgacggcaac accagtcaaa
      301 caaggcgcga gaatcgtcca aaatcttgat gtcgtcagct tgaaacgcct gataccttgc
      361 caaagatttt gctacggcat ccggttcggt aacgccgcgc cgtttgaggc tttccaacat
      421 aaacgggctg tcggagcggg catttttctt cgccaaaaac accaccaaat gactggcggt
      481 atccaaagca tccgccatac cccaagaaaa cggcttgatt gcctgtcgga tttcagggtt
      541 ttgaaccaca ataaactgcc aaggctccga accgaccgaa ctgggcgaca aacgcccgag
      601 ttctaaaata aactgaaaat cctcggcact gattttgcgt gccgcatcgt aatgccggca
      661 tgatttacgg tttttaaatg cggatagaac ctgctctttg cttaatactg tcatcgtgca
      721 tccttttatt acttgtattg caaattgatt tccgatacgg cggcagattc gccaataacg
      781 cccaaacgcc tttcagacgg catttggttc atcgcaaacg cgcctacggg taggcgatat
      841 aggcataagc cgaatggttg tggatagact cgaaattctc gctctcgacg acgaaactct
      901 tgatgcgttt gtcggcaatc agcgaagtag cgacatcgcg caccatatct tccacgaatt
      961 tcgggttttc gtaggctttt tcggtaacgt atttttcatc ggggcgtttg agcaggccgt
     1021 agagttggca gctcgcctgc gcctccacat aatcgatgac ttcctcgata ccgacttcgg
     1081 catcggcagt caggctgacg gtaacgtgcg aacgctggtt gtgcgcgccg tattgggaaa
     1141 tttctttgga acacgggcaa agcgaggtta cgggaatcat gaccttcata ctgtggccgt
     1201 atgccccgtc tttgatttcg cccgtgagac agacatcata atccagtaag gaccgaatac
     1261 cggaaaccgg ggcggttttc ttgcggaaaa atgggaaaga aacgctgatt ttgccggcgc
     1321 gggaatctaa aagcgcgacc atttcggtag tcagcttgcg caattgtgca aaatccaagg
     1381 cttcggcatg ttgctccatc aatgcgacaa aacgcgacat atgcgtcccc ttctgctcag
     1441 caggcaggta aaccgtcatg gtcagacggg caatggtgga ctggatgcct tctgcagttt
     1501 gcagggtaat cggaaagcgc aggtctttga taccgacctg attgatcggc agattgcgca
     1561 aatctcggct ggattgcacg tctgcaatag tgttcatgag ttgtttttcc ttaatattca
     1621 tgtaattgtt gagatgtgtg catggtatgg cgtttccgcc ggggaatata ccgtcaatct
     1681 gcaaatgcga gattatatca ctcgctttta gcagctgcat tgattgtttc tatttttcag
     1741 atagtgataa aatcttcgat tattcacatc tacataggca gagaccaaat gaaattcgcc
     1801 acgaaagcca ttcattccag ctacgattgc gacgaacaca accgcgcgct gatgccgccg
     1861 atttatcaaa acagtatgtt tgcgttgcac gagattggcg aaaatgtgcc ttaccgttat
     1921 tcgcgcctga gcaacccgac ccgtcagatt ttagaagaca ccgttgccga tttggaacac
     1981 ggtgcggcag gttttgcgtt ttccagcggt atggcgggaa ttgatgccgt atggcgcact
     2041 ttcctgcgcc cgggcgatac cattgtcgcc gtcgccgata tttacggcgg cgcttatgat
     2101 ttattggtcg atgtttatca aaaatggggg gtgaacgttg tttttgccga tttaggcaat
     2161 ccggataatt tggacgaact gcttaaagcg cacaaggtca aactggtttg gctggaaacg
     2221 ccgtccaatc cacttttacg cttggtagac atcaaagtcc ttgccgcaaa agccaaagca
     2281 gccggtgcgc tggtcggtat cgacaacact tttgccacgc cgtatctgca acagccgttg
     2341 gatatgggtt gcgattttgt attccattcc gctaccaaat atttgtgcgg ccattccgac
     2401 gtgttgatgg gcatcgtcgt tgccaaaacc aaagaactgg cgcagccttt gcacgatatg
     2461 atggtgcata ccggcgcggt tgccggcccg ctggactgct ggctggtgtt gcgcggcatc
     2521 aaaacactgg ctctgcgcat gaacgcccat tgccaaaacg cactcgaaat cgcgcgccgt
     2581 ttggaagccc atcctgccat tgaaaaagtg ttccatcccg gcctgccgtc tcacgaacat
     2641 tacgaactgg cgaaaacaca aatgcccaaa ggcatcggcg gcgtggttac ggtttatctc
     2701 aaaaacgaca cgcgtgaagc ggcaaacagc gtgattaaaa acatgaaact ggtcaaaatg
     2761 gcttccagcc tcggcggtgt ggaaagtttg gtcaaccatt gctattccca gtcccacagc
     2821 ggcgtaccgc atgatgtgaa aatggaaatg ggcatcaaag tcggtctgct gcgtttctcc
     2881 atcggcatcg aagacgcgga cgatatttgg aacgatattt ccgccgcact cgatacaact
     2941 ttgtaaaccg taaaaatgcc gtctgaaacc atagtttcag acagcatttc aatattgcga
     3001 atcaaatatc aacgcttcag catcttggcc gcctcaatgg cataataggt caaaatccca
     3061 tccgcgcccg cacgtttgaa tgccagcagg ctttccaaaa ccactttatc gccgtccagc
     3121 catccgttgg caaccgccgc ctgcagcatc gcatattcgc ccgaaacctg ataggcataa
     3181 gtcggcacac cgaactcgtc cttcacgcgg cggacaacgt ccaaatacgg caaaccgggc
     3241 ttcaccatca ccatatccgc accttcctga atatcgagcg ctacttcatg cagcgcctca
     3301 tcggtgtttg cgggatccat ctgataggtc tttttatctg ccttgcccaa attgcccgaa
     3361 ctgcctaccg catcacggaa agggccatag aatgcagaag catatttggc ggaatatgcc
     3421 ataatccgcg tgtggatatg tccggcatcc tccaacgcct cgcgaatcgc accgatacgc
     3481 ccgtccatca tatccgacgg agcaaccacc tgcgcgcccg cttcagcgtg gcacaaagcc
     3541 tgcttgacca aaacctctac ggtttcatcg ttcatcacat aaccgttttc gtccgtcagc
     3601 ccgtcctgac cgtgaaccgt ataaggatcg agcgcgacat ccgtcataat gcccagttcg
     3661 ggaaacctct cgcgcaaggc gcggacagtt gacggcacga gtccttcggg attgtacgcc
     3721 tcctgcgcac gctcggtttt gtttgccgta accacgggga acagtgccaa catcggaata
     3781 ccgagcttta ccgcctcttc cgccgtaaac agcagcctgt ccaaactttg acgcttcaca
     3841 cccggcatag aaggcacatc ctcctcgcgc gccgacccct ccaatacgaa caccggataa
     3901 atcaaatcat cggcggtcag cgtgtgttcg cgcatcaggc ggcgtgaaaa atcgtccctg
     3961 cgcatacggc gcatacgcga agccggaaca ttgcggtaag gaaactgcat aagccctcca
     4021 atcatcctgt caacaattca aaccatacgg aagccgccgc aacttgcaga cagcatcggg
     4081 cggcaatcaa aatccgtttt caccggaaag ggtttcggcc cgccgaaatg ccgcgccgaa
     4141 ggacggcgtc ccgcccatta aagaacaata gcaaaacaac agcttcaggc tgtcagcctt
     4201 cgatattctt acgccacaat accaaaagtt tcccgatatg ctgaacaggt tgcgcgtcaa
     4261 ccgcctcaca taaggcagtg cagatttcga tacgttcggc acggtcgtcg ccgaatacgc
     4321 gcactttaat cagctcatgc gccgtcaatg ccgcatcggt ttccttgatg accgcgtccg
     4381 tcagaccctg ctgaccgacc atcacaacag gatggagatg gtgcgcgcgc gctttcagtt
     4441 ccaaaatttc tttggtgttc aatttggtat ccgtcatttt cctactgctt gaataaaaag
     4501 aataatgcgg cattgtacgc gatttggcac gacatcggca aaattaacgt acaatacgcg
     4561 gtttccattc cgacccgcga accactatgg ctgtacgttc caaatcctca aaagcgtggc
     4621 tgcacgaaca cgtcaacgac cactacgtcc atatggcgca aaaagacggc taccgcgccc
     4681 gtgccgcata caaacttttg gaaatcaacg aaaaagacaa attaatcaaa cccggcacgg
     4741 tacttgccga cttgggcagc gcaccgggaa gctggtcgca ggttgccgcc aagctgacgg
     4801 gtacttccgg agcagttttc gccttggaca tcctgcctat ggaagccata gggggcgtct
     4861 ccttcattca gggcgacttc agggaaaacg acgttttggc gcaatttgaa ggcttattaa
     4921 acaaccgccc cttagacctt gtaatctgcg atatggcgcc caatatgtcg ggaaacgccg
     4981 taagcgatca ggcgcgcagc ttttatttgt gcgaactggc tttggacttc gcctcgcaac
     5041 acctgaaaac cggcggcagc tttttggtca aagtctttca gggtgcaggc tatcaggaat
     5101 acatggcagc catgcgcgaa attttcggca cggtgcagac gcgcaaaccc gaagcctcgc
     5161 gcaatcgctc cagtgagatt tatttattgg gcaaaaataa acgctgacaa tacagacggc
     5221 gtgctttaca atcatttccg ttttacacct caattatgga gccttgctaa gtggggaaca
     5281 cctttaaatc aatccttgtc tgggtcgcct tgggtatcgg cctgatggct gcgttcaacg
     5341 ctttagacgg taaaaaagaa gacaacgggc aaatcgaata ttctcagttc atccaacagg
     5401 tcaacaacgg cgaagtatcc ggcgtcaaca tcgaaggatc cgtcgtcagc ggctacctga
     5461 ttaagggcga gcgcaccgac aaaagcactt tcttcaccaa cgcgcctttg gacgacaacc
     5521 taattaaaac actgctcgac aaaaacgtcc gcgtaaaagt aacgccggaa gaaaaaccga
     5581 gcgcgctggc tgccctgttt tacagcctgc tgcccgtcct gctgctgatt ggcgcatggt
     5641 tctacttcat gcgtatgcag acgggcggcg gcggaaaagg cggcgcattc tcattcggta
     5701 aaagccgcgc ccgcctgctg gacaaagatg ccaacaaagt gacctttgcc gatgtcgccg
     5761 gctgcgacga agccaaagaa gaagtacagg aaatcgtcga ttacctcaaa gcgccgaacc
     5821 gctatcaaag cctgggcggg cgcgtgccgc gcggcatcct gctggcgggc agcccgggta
     5881 cgggtaagac gcttttggcg aaagcgattg caggcgaagc cggcgtgccg ttcttcagca
     5941 tttcaggttc cgactttgtc gaaatgttcg tcggtgtcgg tgcgagccgc gtccgcgata
     6001 tgttcgagca ggcgaagaaa aacgccccct gcatcatctt tatcgacgag attgacgcag
     6061 tcggccgcca acgcggcgca ggtttgggcg gcggcaatga tgagcgcgag caaacattaa
     6121 accaattgtt ggttgaaatg gacggttttg agagcaatca gactgtaatt gtgattgcgg
     6181 caaccaaccg ccccgacgta ctcgatcctg cgctgcaacg ccccggccgt ttcgaccgcc
     6241 aagtggttgt ccccctgccg gacatccgag ggcgcgaaca gattttgaac gtccattcta
     6301 aaaaagtgcc tttggacgaa tctgtggatt tattgtccct cgcgcgcggc acgccgggtt
     6361 tttccggcgc ggatttggcg aacttggtca acgaagccgc cctgtttgcc ggccgccgca
     6421 ataaagtcaa agtcgatcag agcgattttg aagacgccaa agacaaaatc tatatgggtc
     6481 cggaacgccg cagtatggtg atgcacgaag acgaaaaacg tgcgacggcg tatcacgaat
     6541 ccggacacgc gattgttgcc gaaagcctgc cctttaccga ccccgtccac aaagtaacca
     6601 ttatgccgcg cggacgtgcg ctgggtctga cttggcagct tccggagcgc gaccgcatca
     6661 gtatgtataa agatcagatg ttgagccagc tctccatcct gttcggcgga cggattgccg
     6721 aagacatctt cgtcggacgc atctccaccg gcgcatcaaa cgactttgaa cgcgcaaccc
     6781 aaatggcgcg cgaaatggta acgcgctacg gcatgagcga caaaatgggc gtgatggttt
     6841 atgcggaaaa cgaaggcgaa gtcttcttgg gacgcagcgt aacccgttcg caaaacattt
     6901 ccgagaaaac ccagcaggac atcgacgcgg aaatccgccg gattttggac gagcaatatc
     6961 aggttgccta caaaatcctc gatgaaaacc gcgacaagat ggaaacgatg tgcaaagccc
     7021 tgatggaatg ggaaaccatc gaccgcgatc aggtactgga aatcatggcg ggcaaacaac
     7081 ccagcccgcc caaagattac agccacaacc tgcgcgagaa tgcggacgcg gcggaagata
     7141 acgcgccgca cgctccgact cgggaagaaa ccgaagcacc tgccccggca gacaccgctt
     7201 cgacagagtc cgagcagcag cctgaaaaca aggcttaact tcccgaacaa acggcagccc
     7261 gcaagctgcc gttttcccat cccgttttca gacggcattt tccactcaat gccgtctgaa
     7321 acgccaaagc cgtatcggtt tttacgaatc tgcgatatgg cggtttaaat tgaaatcaga
     7381 acggggcaag gctgtaccgg tttaaaatta aaccgctata ataccgccct tcgaatacac
     7441 gcccgcattg gtgatgcccg tgaaaaaagt tgaaaaaaac atcttggtgc tgcacggcgc
     7501 ggacaaaatg ttcgagctgg tcgacaaggt tgaagactat ccgcactttc tgccgtggta
     7561 cagcaagacc gaagtcatcg ggcgtagcgg caacgaactg aaggcgcggc tgtttatgga
     7621 ttatatgcac gttcgccaat cgtttgccac gcacaaccgc aacattccgg gcagggaaat
     7681 ccgtatggaa ctgctcgagg gtccgttcaa aaccttacgt ggaacgtgga aatttatcga
     7741 tttgggcgac gatatgtgca aaatcgaatt caatttggaa tacgattttt ccaatgccgt
     7801 tttgtccgcc ttaatttccc ccgtcttcaa ccacctttcc accacgctgg tcgaagcgtt
     7861 cgtcaaagag gcagaccgcc gttatgcttg aaattgagat tgtgtacggg ctgcccgacc
     7921 gacaggtttt gaaaaccatg cagcttgccg agggaacaac cgtccgcgcc gccgcactgc
     7981 aaagcggttt ggacggcata tttgaagatt taaacctgca ttccgcgcct ttgggcattt
     8041 tcggcaaagc cgtcaaagac gacacgccgc tgcgcgacgg cgaccgcatc gaagtgtacc
     8101 gcccgctgtt gatcgacccc aaagaagcgc gccgcaaacg cgttcaaaat caagaagaat
     8161 aaccatgccg tctgaagcct tcagacagca ttcgacagaa acacggaaaa tatcatgtca
     8221 aacacaatca aaatggttgt cggcttgggc aacccgggca aagaatacga acagacacgc
     8281 cacaatgcgg gtttttggtt cctcgacgaa ctggcgtgga aatggaaggc ttcgtttaaa
     8341 gaagaaaaaa aattcttcgg cgaagtcgcc cgtgctaccc tgcccgacgg cgatgtctgg
     8401 ctgctcaagc cgaccacgtt catgaaccgt tccggacagg cagttgccgc ccttgcgcag
     8461 ttttataaaa tcaaacccga agaaatcctc gtcgtccacg acgaactcga cattccctgc
     8521 ggacggatca aattcaaact cggcggcggc aacggtggac acaacggctt gaaagacatt
     8581 caggcacggc tcggcacggc cgactattac cgcctgcgcc tcggtatcga ccacccgggc
     8641 gaccgcaacc tcgtcgtcgg ctatgtcctg aacaaaccca gtgcggaaca ccgccaacag
     8701 attgatgatg ccatcgccaa atccctgcag gccatacccg acatcatttc cggcaaatgg
     8761 gaagaagcaa cacgtttcct gcacagcaaa tgacccaatg ccgtctgaag ccctttcaga
     8821 cggcattccc gccatcagcg agaacccgca ctatgtccga actcaaccgg ctcatccaaa
     8881 ccgaatccgt tgccgtcatt gaagaaactt taaattttct gctctacgaa tgcagcatag
     8941 acgatgcccc ctccgccgaa gaaattgccg tttggcgcga tatgctggcc gcacgcggcg
     9001 gaaaattcct gcgcctgtcc aaaatctgcc aaacgtggct ggacgaggag gcggcatgaa
     9061 gctgccgcgc aaccgcttca gcctgctttc cgcattgtgg tttgccggcg gcatctattc
     9121 gctgctcttc aaagctgccg acaccgcgcc gccgccgttt ccgcatttcg acaaagcagc
     9181 acaccttgcc ctgtttttcg cacaaatctg gcttttgacc aaagcattca aaaccggaaa
     9241 acttcccatc ccctaccgca gcctgatggt ctttgccctc tgtttcgccc tcttcagcga
     9301 atgcgcgcag gcatggttta ccgcaacgag aaccggcagt ttgggcgatg ttcttgccga
     9361 tatggcaggt acggttctcg cactctttgc cgcccgcgcc gccgaccgcc cggactgaat
     9421 cggtttattt tccccaaaca ggatgcacct cttcccgata catcctattt ttccccttac
     9481 atatcaatcc catctcataa acaaaaccga caaatcgttt acaatatatt tacactacat
     9541 cagattacaa atatactcga cccagttgca gaatcggcgg tttcacaaac cgtttcggat
     9601 tgcacgacac aaaagaaaaa ccgattttat tgccttaaag gagtattcat gaacctgcat
     9661 gcaaaggaca aaacccagca tcccgaaaac gtcgagctgc tcagtgcgca gaagccgatt
     9721 accgacttta agggtctgct gaccaccatt atttccgccg tcgtctgttt cggcatttac
     9781 cacatcctgc cttacagccc ggatgccaat aaaggtatcg cgctgctgat tttcgttgcc
     9841 gcactttggt ttaccgaagc cgtccacatt accgtaaccg cactgatggt gccgattctc
     9901 gccgtcgtac tcggtttccc cgacatggac atcaaaaagg cgatggctga tttttccaac
     9961 ccgattatct acattttttt cggcggcttc gcgcttgcca ccgccctgca tatgcagcgg
    10021 ctggaccgta aaatcgccgt cagcctgttg cgcctgtcgc gtggcaatat gaaagtggcg
    10081 gttttgatgt tgttcctcgt taccgccttt ctgtccatgt ggatcagcaa caccgccacc
    10141 gccgcgatga tgctgcctct agcaatgggt atgctgagcc acctcgacca ggaaaaagaa
    10201 cacaaaacct acgtcttcct cctgctcggc atcgcctatt gcgccagcat cggcggcttg
    10261 ggcacgctcg tcggctcgcc gcccaacctg attgccgcca aagccctaaa tctggacttc
    10321 gtcggctgga tgaagctcgg cctgccgatg atgctgttga ttctgccctt gatgctgctc
    10381 tccctgtacg tcatcctcaa acctaatttg aacgaacgcg tggaaatcaa agccgaatcc
    10441 atcccttgga cgctgcaccg cgtgatcgcg ctgttgattt tccttgccac agccgccgcg
    10501 tggatattca gctccaaaat caaaaccgcc ttcggcattt ccaatcccga caccgttatc
    10561 gccctgagtg ccgccgtcgc cgtcgtcgtc ttcggcgtgg cgcaatggaa ggaagtcgcc
    10621 cgcaataccg actggggcgt gttgatgctc ttcggcggcg gcatcagcct gagcacgctg
    10681 ttgaaaacat ccggcgcgtc cgaagccttg ggacagcagg ttgccgccac cttttccggc
    10741 gcgcccgcat ttttggtgat actcatcgtc gccgccttca ttatttttct gaccgagttc
    10801 accagcaaca ccgcctccgc cgcattgctt gtaccgattt tctccggcat cgctatgcag
    10861 atggggctgc ccgaacaagt cttggtattc gtcatcggca tcggcgcatc ttgtgccttc
    10921 atgctgccgg ttgccacacc gcctaacgcg attgtgttcg gcacgggctt aatcaagcaa
    10981 cgcgaaatga tgaatgtcgg catactgctg aacatcctct gcgtagtatt ggttgctctg
    11041 tgggcttatg ctgtactgat gtaaaccatc gacctaaaca acaagaccgt ctgaaagaat
    11101 atttttcaga cgaccttgaa gtttgtcgtt cagacacaat ttgtcgaatc attcaaaacc
    11161 ggattctaac gaaaggaaac ccatgattat cctgcacacc aacaaaggcg acatcaaaat
    11221 cgaactcgat ttcgacaaag cccccgttac cgccaaaaac ttcgagcaat acgtcaaaga
    11281 cggcttctac gacggcgtaa tcttccaccg cgtcatcaaa ggcttcatga ttcaaggcgg
    11341 cggcatggat gaaaacatga acgaaaaaga aacccgcgat ccgattcaaa acgaagcgtc
    11401 caacggcctg cccaacgata aatacaccat cgccatggca cgcacttccg acccccattc
    11461 cgccagcgcg caattcttca tcaacactgc cgacaacgct ttcctgaact tccgttctaa
    11521 agagctgtac ggcaaaaccg tcgtccaaga ctggggctat gcagtattcg gtaaagtcgt
    11581 tgacggtttt gacgttgtcg atgccatcga aggcgtatcc accaaacgcc acggctacca
    11641 tgacgacgta ccgagcgaac ctgtcgtcat cattaaagcc gaagcggtat aaaccggcaa
    11701 tccgaaagca gcctgcacaa aggctgcttt ttttgtttca ggcggcataa aatccaagca
    11761 catatgccgt ctgaacacct cccgcaagcc ttcggacaat gttgcaacgt gttaaaatag
    11821 cctcctttcc tgaaaccaca acaaggaaac catcatggca aatattgccc gcgacatctt
    11881 caaagcctac gacatccgag gcattgtcgg caaaaccctg actgacgatg ccgcctatct
    11941 catcggcagg gccatcgccg ccaaagccgc cgaaaaaggc atgacccgca tcgcgctcgg
    12001 acgcgacgga cgcttgagcg gtcccgaact gatggagcac atccaacgcg gctttaccga
    12061 cagcggcatc gacgtcctca acgtcggtat ggtcgccacg cccatgctct attttgccgc
    12121 tatcaacgaa tgcggcggca gcggcgtgat gattaccggc agccacaatc cgcccgatta
    12181 caatggtttc aaaatgatgc tcggcggcga cacgctcgca ggcgaagcca ttcaagaact
    12241 tttagccatt attgaaaaag acggttttgt tgccgccgac aaacaaggca acgtaaccga
    12301 aaaagacatc tccggcgaat accacaacca catcgtcggc cacatcaaac tcaaacgccc
    12361 catgaaaatc gccatcgacg caggcaacgg cgtaggcggc gcattcgcag gcaaactcta
    12421 caaaggcttg ggcaatgaag ttaccgaact tttctgcgaa gtggacggca acttccctaa
    12481 tcaccacccc gacccttcca aaccgaaaaa cctgcaagac ctcatcgtcg agctcaaaaa
    12541 cggcgaggcc gaaatcggct tagcatttga cggcgatgcc gaccgcttgg gcgtggttac
    12601 caaagacggc aacatcatct atccggaccg ccaactgatg cttttcgcgc aagacgtgtt
    12661 gagccgcaat cctaaagcca aagtgatttt cgacgtcaaa tccacccgtc tgcttgcgcc
    12721 ttggattaaa gaacacggcg gcgaacctgt gatggaaaaa accggccaca gctttatcaa
    12781 atccgccatg aaaaaaaccg gcgcgccggt tgccggcgaa atgagcggac acatcttctt
    12841 caaagaacgc tggttcggct tcgacgacgg tctgtacgcc ggcgcacgcc tcttggaaat
    12901 cctgtctgcc tccgataatc cgtccgaagt gttaaacaac ctgccgcaaa gcatttccac
    12961 gcccgaactc aacatcgccc tgcccgaagg cagcaacggc catcaggtta tcgacgaact
    13021 cgccgccaaa gccgaatttg aaggcgcaac cgaaatcatc accatcgacg gtctgcgcgt
    13081 tgaatttccc gacggcttcg gtctgatgcg tgcttccaat accacgccga ttctggtgtt
    13141 gcgttttgag gcggatacgc aagaagccat cgagcggatt caaaaccaat tcaaagccgt
    13201 catcgaaagc aatccgaatc taatctggcc tctgtaaaca caggaaaaat gcttaatcaa
    13261 atcttggtag attggatttg gcttaaaaat ctccgcgtcc gttcgtgttt ggggtggtcg
    13321 aacaactctt tcgggctgcc ctgctctacg ataacgccgc cgtccatcac gacaacggtc
    13381 gtggcaactt ccagcgcgaa cttgatttcg tgggtaacga cgaccatcgt ccaaccttcc
    13441 cgcgccaatt ccttcatggc gttcaacacg tcttgcacca actcggggtc aagcgcggaa
    13501 gtgggttcgt caaacaacat cagctcgggc tgaatcgcca atgctcgggc aatgccgacg
    13561 cgctgctgct gaccgccgga aagctggtag ggatacaaat ccactttgtc gcccaagccg
    13621 actttttcca gcagtttcag agcctcttcg cgcgcttggg cggcaggctt gccctgtacg
    13681 gcaaccggtc cttccatcac gttttccaag gcggttttgt gcggaaagag gttgtattgt
    13741 tgaaacacca tgcctgattt gcggcgcagt gccaaaatat cgtgtttgct tggtttttta
    13801 gaaaaatcga ttttcagcgg tcgctcgttg tcgaactcga tttgtccgtc ttcgggcatt
    13861 tccaacgcgt ttaggcagcg cagaaacgtc gttttgcctg agccggaagg cccgaggatg
    13921 acgaccacct gccctttgca cacatccaaa tcgatgccgc gcaaaatagt gttttcgcca
    13981 aaggtcttat ggatattgcg gattttaatc atgacaactc cttatttggc gacgtagcgg
    14041 tcgaaacgtt tttccaaacg cgcctgaatc aggaacagca ctttacaaaa acaccagtaa
    14101 accaaagcgg cttcgatata gacgggcaaa aagtcataag tgcggtttgc cgtttcctgc
    14161 gcgacgcgga ataattccgt taccgtcacg actgccgcga gcgaggtgtt tttaaacaaa
    14221 ccgataaact cgttgctcaa aggcggcacg gcaacgcgga atgcctgcgg cgcgacaatg
    14281 cggcggaacg tctgcatata ggtcatgccg atggagaaac ctgcttccca ttggccttta
    14341 ggtacggaca aaattgccgc gcgtatggtt tcggaagcgt atgcgccgac attgagcgaa
    14401 aagccgatga tggcggcagg aatcgggtcg atatagatgc cgacggaagg cagcccgtaa
    14461 aacacaatca caagctgaac caacagcggc gtaccgcgaa tgacggaaat ataaaattcc
    14521 accaatttca gcaggatttt ccgcacgatg ccgccggcgg gcataatccg caccaaagcc
    14581 acggctaccg caatcatcat accgataacg aaagaagctg ccgccaaagg cagagagacc
    14641 gcgaagccgg ctttgaccat aggcaaaaac gcgctgacaa tcatatcggc gcgtgtttcc
    14701 gtcataaacg gcagcgaagc aaggaaatta ttgaacactg atgtcttttc cgaagaattg
    14761 ttcgcccagt tttttcagcg taccgtcggc tttcagctcg ttgattgccg tactgaattt
    14821 cgccacggct tcgtcattgc ccttgttgac aatcaggccg gaaccgactt tttcatcggc
    14881 aggtgcggac caaacgattt tcacgcccgc attcgggttt ttcttcagat agtccaaaac
    14941 cgccaattcg tcgttcaggg ttgcatcggc acgtttttgt tcaatcaggg ttagcgattg
    15001 cgccaaaccg tcaacagcca ccaaatctgc gcctgcagct ttggcttttt cgccgtagtt
    15061 gctggtcagg gattgtgcgg ttttcacgcc tttgatgtcg gctatagatt tgatgttgct
    15121 gtcgttacgg acaaccaata cggcaccgct ccagctgtaa ggatcggatt tgtcgaatgt
    15181 cgcttggcgt tcggggctgg tcagaccgac ttggtttgcc accacgtcga aacgccccgc
    15241 cttcaaaccc gccatcatcg aatcccattg cgtttcttta aactcgactt tcacgcccag
    15301 tttttccgcc acggcgcggg ttacttccac atcgtaaccg gtcagtttgc cgtccttgtc
    15361 gtggtaggta aacggtgcgt aagtgccttc cgtgccgacg gtaaccgtgc ctttattgtt
    15421 gatgcgctcg attaaagaac cggaaactgc cgattgtgca ggcgcggaag atgctccgct
    15481 gccgccttcc gaaccgccgc aggccgccaa aaccaatgcg gcaataccgc cgagtacgaa
    15541 ttttttcaac atcatcctct cctgtaaaaa gtgtgcaagt gcggcattct aagtgaaatt
    15601 gcggcaaaca caaagaatac tttttttata tggttattcc aaatcggaat ggaaaagata
    15661 tgccgaagcc gccggaagcg gaaataaagg gggcgggaaa ggcggcgggc ggcacgaaac
    15721 cgaaaagcgg catcaggtat gtaaacggcg gattcacgca cgccgttccg gcaaaaatcc
    15781 gcctgccgat tcggtgctat aatctatccg aaacagatga caccaaaaag gaacatctca
    15841 tgaacacgcc ccaatccgcc atcattcccg accacgccca agccggcatc tttatcgaag
    15901 ccgacttcgc cgccaatcgc ctcaacgata ttaaagccgc ctgccgcgct tcgctcgacg
    15961 cgttaactgc cttgaaagcc cgctttccgg acgatatttt gggtctgacc atcgccttcg
    16021 gcagcaaagc ctgggcaaca ttcggacata cggacgaagg cagtgaaatc aaacccttct
    16081 ccgaaatggg caacgggctt gcgccgtcca cgcagcacga tatgtccatc cacatccaat
    16141 ccttccgcca aaacgccgcc tacgcgcttg cccaatccgt tttgggcgcg ttcggcgaca
    16201 gcatatgcgt cgcgtccgaa gaacacggtt tgcgcctgta tcaggatcgc gggctggacg
    16261 gtttcgtcga cggcacggaa aacccgcagg gcgatgaaaa tgtccgcgaa gtcgccatca
    16321 tccccgaagg aaaacccgat gcgggcggca gctatgtcct gctgcaaaaa tacctgcacg
    16381 atttgaaaaa atgggatgcc gtccccgtcg ccgaacagga ggcctcggtc ggacgcggca
    16441 aggaaaccga cgacgaattc ggccgcgatg tccgcctgcc cgattcgcac ctcggccgcg
    16501 tcaacctgaa agaaaacgga gtcggcctga aaatcgtccg ccgcagcctg cccttcggca
    16561 aaatcagcgg cgaacacggt ttgatgttta ccgcatactg ccgcacgctg cacaatatcg
    16621 aagcgcaatt attgagtatg ttcggcgata cggacggcaa aaccgacctg ctgctccgac
    16681 atctctccgc cgccgtttcg ggcggatact actacgcgcc gtctgtcgaa cgcctgcaaa
    16741 acctctaagc cggacgatgc cgtctgaaac catttttcag acggcatttt cccgttttac
    16801 cgtccaaccg ttttccgagc gcgcactatg tctgcaagca tccaagcatt cgacccgcta
    16861 accgtcccca tttccggcac caacctgatt gaggcctctg ccggcaccgg caaaacctac
    16921 ggcattgccg ccctgtttac acgcctgatc gtattagaac aaaaaaacgt cgaacgcgta
    16981 ttggtcgtaa ccttcaccaa agccgccacc gccgagctga aaacacgcct gcgcgggcgt
    17041 ttggacgatg tgttacaagt tttagaaagc aaaggaattg ccaaacttgg agacgacacg
    17101 ctttcagacg gcattgccgc ctactgcgcc gaacaccacg aaggcgacac cttcctgccc
    17161 gaactcttaa aacaggcttt gcaaaaagag ggccggacgc gtctgattgt ccgcctcaag
    17221 gccgccatcg ggcaattcga caacgccgcc atctatacca tccacggctt ctgccagcgc
    17281 atcctgcgcg actacgcctt cctgtgccaa gcaccgttcg atgtcgaaat gaccgaagaa
    17341 gacggcgacc gcctgcttat cccggcgcaa gatttttggc gggaacgcgt cagcaatgat
    17401 ccggtgcttg ccgcattggc gcttaaacgc aaagctgtgc cgcaaaccgt ccttgcccaa
    17461 atttcgcgct atctttcccg cccgtacctg aatttccgcc gtccgcaggc ggatttgaaa
    17521 caggcgcagc gcaacgccga aacctcatgg caaaccatct gccgcctgct gccagaactg
    17581 gaagccggct tttggcgcat tcatcccgac ctcaacggca acagttaccg caaaaacagc
    17641 ttcggcaacc tttttaaaga actggcacaa aaatccgccg ccggacaact gccctttctg
    17701 gacaaagaca cacacgacag actcttgaaa cttgcatccg acaaactcga agccggactg
    17761 aaaaaaggca aaacgcccga tgcggcagta tttgccgaat tgcagaaact ggcagacttc
    17821 gggcgcgatt tgaacgcact cgaagaagcg gaagaagcaa caatgatccg gctgcaactg
    17881 gatttaatcg aatacctcaa ccgcagcctt gccgagatga aaaaatcgcg ccgcgaacgc
    17941 ggtttcgacg acctgctgct cgatgtccac accgcgctga ccgacaatcc gcacgccgac
    18001 actctcgccc gcgccgttgc cgaaaactgg gaaaccgcgc tgatcgacga gttccaagac
    18061 accgacccgc tgcaatacga aatcttccaa aagattttca tcgcccaaaa ccgaccgctg
    18121 ttcctcgtcg gcgatcccaa acaggcgatt tacagctttc gcggagcgga catttacgcc
    18181 taccttcagg cggcggaaga cgcgcggcac cgctacacgc tcgccaccaa ctaccgcagc
    18241 cacgccgcgc ttatcggcag cataggcgcg ctgttccgcc tcaaagaacg cccgttcgtt
    18301 ttggaaaaca tcggctattc ggaagtcggt gcggcgcgtg ccgaaagcag gctgtccccc
    18361 gaacgtcctg ccgtacaggt tcgttggctg cacgaaaacg acaatgaaaa agccaacaaa
    18421 gacgttttgc gccgccgtgc cgccgactat tgcgccgacg aaatcgccca cgcgctcaac
    18481 gaagccgcca gaggccgtct gaatttcaaa ggctgcccgt tgcagtcggg cgatattgcc
    18541 gtgctggtac gcacgcacaa cgaggcggtg atggtttccg ccgccctgaa aaaacggcag
    18601 gtgcaaagcg tcctgctttc gcgcgaatct gtgttcgcct cgcccgaagc cgccgccctg
    18661 tccgcactca tcggcttctg gctcgaaccg cgccgcgccg gaacgctgcg ttttgtcctg
    18721 acaagcagca tattcggtta tgacgcgcag caattgcacg acttcaacca aaacgaaagc
    18781 gagattttgc attgggcgga atctgcccga accgcactcg acaactggaa taaatacggc
    18841 attttcgccg ctatgcagca attttcccaa acacacggca tcgaaacgcg cctcttaagc
    18901 cggaacaacg ggcgcagtct gaccaattat ttccaactgc tcgaactgct tgccgccgaa
    18961 gacgcgcaaa accgcaaccc cgccgcgctg cacaaatggc tgggcgacca aatcagcctt
    19021 gccgaaaaca acagcggcga caaccgcgcc atccgtctgg aaagcgacga agatttggtc
    19081 aaaatcgtta ccatgcacgc ctcgaaaggt ttgcagtatc cgctggtgta ctgcccgttt
    19141 gcgtgggacg cgcaagatac cggaccgtcc gactggcaaa tcctccacca aggcgcaaac
    19201 cgaaccgaac tgttggcaaa ggcgcaactg tcggaagacg aacagaaaca atacgccgat
    19261 gaagaaatgg cggaacgcct gcgcctgctt tatgtcgcgc tgacgcgtgc cgaggaacag
    19321 ctcaacatct acgccgcgta ctccaccaat actgccgaca accccctcgc ctacctgatt
    19381 gaaggctcgc cccaagacag ccgtgaaacc gtccgccgtg cctatgcgtg tgaaaaagac
    19441 ggcatcgcga tgctcaaacg caactggcgg cgcgtggcgg acaacgcccc ttccggcaca
    19501 aatttcgcct tcaccgaaaa tgcgccgccc cctgccgcct atcgcagcaa cgccggtcaa
    19561 accgccgaat ttgccgcaaa cagcattccc gaacgcggat tccgatttgt ccgccacact
    19621 agctttaccg ccttaagccg ccatacccaa acgcccgacg gcggcgaaga agatgcctgc
    19681 ccgtccttgg atgccgccga aacctcggtg ccggcgatgc cgtctgaaac gccgacggct
    19741 tcagacggca tatcgataca cgattttccg aaaggcacgc aggcggggct gtgcctgcac
    19801 gaaattcttg aagatttcaa attcggacaa gcagccgccg gacaggaaac cctcattgcc
    19861 gacaagctga aaaaatacgg ttttgaagaa atatggctgc ccgccgttgc cgaaatggcg
    19921 gaagcctgcc gcaaaacgcc gctgacgggg gcatacgacc tgtccgacat ctcacccgag
    19981 tgccgctgtc ccgaaatggg ctttaccctc cacaccgaag acttcagcct caaacgcctg
    20041 cgcgactggt ttgcccgcga cgacatcagg ctgcccgaag tctgccgtgc cgctgccgaa
    20101 acgctcgact tccacaccgt caacggcttt ttaaacggct ttgtcgatat ggtctgccaa
    20161 gaccccgacg gcaatatctg cgtcatcgac tacaaatcaa accacctcgg tacggacgca
    20221 tccgcctaca cgcaacaggc aatggacgaa gccgtcgcac accaccacta ttaccttcag
    20281 gcactgattt acgccgttgc cgccgcacgc tacttcaagc tgcgcggaca accgcccgcc
    20341 gccgtttccg tccgctacct gtttttgcgc ggattggatg gcaaaggcgg cggcgtttgg
    20401 cgttgggaca tagatgccgc cgctttggaa cagataaaat aacttcccgt cctttcggac
    20461 aacaaccgca ggaaaaccct atgaatatca aacacctgat taccgccgca ctcattgcct
    20521 cagccgcctt tgccgcgcag gcagccccgc aaaaacccgt atccgccgcc caaaccgcgc
    20581 aacatccggc cgtttggatc gatgtccgtt ccgaacagga atttagcgaa gggcatttgc
    20641 acaacgcggt caacatcccc gtcgaccaaa tcgtccgccg catacacgaa gccgcgcccg
    20701 acaaagacac gccggtcaac ctctactgcc gcagcggacg gcgtgccgaa gccgcccttc
    20761 aagagctgaa aaaagcaggt tatacaaatg ttgccaatca cggcggttat gaagacctgc
    20821 tcaaaaaagg gatgaaatga cgcaatgccg tctgaaagga caggctttca gacggcatac
    20881 ggcgttccgg cacgcggcgc aacatcgggc gcggcgcgcg ccgccccgtc cgaagcctga
    20941 taaacaaacc gataaggaac aatgatgttt cacgacacat aaagcaccgc cctatgtgtt
    21001 gccctgattt ggaaagggtt acacccctcc cgaataaagt ctgattctac cgtttcaacc
    21061 aaaaaggaaa aaggaaatac gatgaaacac atactccccc tgattgccgc atccgcactc
    21121 tgcatttcaa ccgcttcggc acatcctgcc agcgaaccgt ccacccaaaa cgaaaccgct
    21181 atgaccacgc ataccctcac ctcaaaatac agttttgacg aaaccgtcag ccgccttgaa
    21241 accgccataa aaagcaaagg gatggacatt tttgccgtca tcgaccatca ggaagccgcc
    21301 cgccgaaacg gcttaacgat gcagccggca aaagtcatcg tcttcggcac gcccaaagcc
    21361 ggcacgccgc tgatggtcaa agaccccgcc ttcgccctgc aactgcccct acgcgtcctc
    21421 gttaccgaaa cggacggcaa agtacgcgcc gcctataccg atacgcgcgc cctcatcgcc
    21481 ggcagccgca tcggtttcga cgaagtggca aacactttgg caaacgccga aaaactgata
    21541 caaaaaaccg taggcgaata agcccgtcgc cgacatatgg cggatggaaa tcaaatgcac
    21601 acgcccctca ttcccacgaa gtcgggaatc caatcggttg ggttaccgtg attttcaatc
    21661 attgccgaaa cggacgattc cggattccca cccttgcggg gatgacgggc gtgtgtgttt
    21721 ttgccacatc acaggcaact ttgccgcaaa tcttatctgt cgccggtatg ccttcttgtt
    21781 tcaatccgcc acaaaccaaa tgccgtctga aacccgattt cagacggcat ttccgtttat
    21841 tccctgctgt cgcggcaaat atcgatggct tcctgcaaac tcgaaacgcc gtagattttc
    21901 aggttcggaa actctttggc gttgcgcggc atattggctt tggggacgat ggcgcgtttg
    21961 aagccgagtt tttccgcttc tttgagccgc tcttgcccgc gtgcgacggg gcggacttcg
    22021 ccgcttaagc cgatttcgcc gaaaaccacg gttttttcag gcatagggcg gttgcggaag
    22081 ctggaaagca tcgcgaggat gaccgccaaa tccgccgccg gttcgccgat tttcacgccg
    22141 ccgacggcgt tgaggaacac atcctgatcg aaacaggcga tgccgccgtg gcggtttaac
    22201 acggcaagca gcatcgcaag acggttttgt tccagtccga cggtgaggcg tttgggcgtg
    22261 aagccgtgcg cgtcatcgac caatgcctga atttcgacca aaagcgggcg gctgccttcc
    22321 tgtgtaacca aaacgcacga gccgggcgta tcgtcgcggt agctggcgag gaagatggcg
    22381 gacgggttgg acacaccttt caaaccgttt tccgtcatcg cgaacacgcc cagttcgttt
    22441 gccgcgccga agcggttttt gatggcgcgt atcatgcggt agttggaatg ttggtcgccc
    22501 tcgaaataca gcacggtatc aaccatgtgt tccagcacgc gcgggccggc aatcgcgccg
    22561 tctttggtca cgtgtccgac cagtatcatg gcgatgccca tctgtttcgc catgcgcgtc
    22621 agttgggcgg cacactcgcg cacctgcgac acggagccgg gggcggacgt gatttggtcg
    22681 gaatacatgg tttggataga gtcgatgacg acaacttcgg gctgatgctg tttcaaggcc
    22741 gcctgaatcg cttccatgcg gatttcggca agaaggttta cgccgtcggt cggcagttcc
    22801 aaacgctgcg cgcgcagggc gacttgttgg gcggattctt cgccggaaac gtatagcact
    22861 ttacggcttt gcgccatttt ggcgatggtt tgcaacagca gcgtggattt gccgatgccg
    22921 gggtcgccgc cgagcaggat gaccgcacca tcgaccaaac cgccgcccaa tacgcggtcg
    22981 agttcgccca taccggtcgg attgcgcggc acttcggtgg cggtaacggc ggagagggat
    23041 tggacggtcg aggtatccgc cgcccaagat tggaaacggg cgtttttcgg ctcgggcgcg
    23101 gcaaggcttt cctgaagcgt gttccactcg ccgcaatgcg ggcatttgcc ctgccatttc
    23161 ggcgaagtgc cgccgcattc ggtgcattgg taaagggttt taagcgtttt tgccatcggt
    23221 tttccctgca tctgaaaaca aaaatgccgt ctgaacatag ggacggttgg gattgcgcca
    23281 atacgacaac gccgccgtca tgtactatcc gtacacggcg gatattgccg cattgcctta
    23341 tttttaccct aaccggccat ctctcggctt cagacggcat atcgggcggt ttcctgccgg
    23401 cttacccgcc gtgatactgc cgagacagct cgtgtacggt atcgactaag attttggcgt
    23461 gttcggggtc ggcgtgttgg ttgatgccgt gtccgaggtt gaagacatgg ccgctgccgt
    23521 gtccgtagtc ggctaggata cgtgcgacct cggtgcggat ggattccggc gtaccgaaga
    23581 gggcgaacgg gtcgaagttg ccttgcaggg cgacttgctt gccgacgcgg cggcgtgctt
    23641 cgccgatgtt acacgtccag tccaagccca atgcgtctgc gccgatttgg gccatacttt
    23701 ccagccacag cccgccgcct ttggcaaata cgataacagg cacgcggcgg ccttcgcttt
    23761 cgcgtttgag tccggcgacg atctggcgga tgtatttgag gctgaattct ttaaacgccg
    23821 catcgctcaa cacgccgccc caagtgtcga aaatctgcac cgcctgcgcg cccgcgtcga
    23881 tttgggcgtt gaggtaggcg gtaacggctt gggcgttggt atcgaggatt ttgtgcagca
    23941 aatcggggcg cgagtacatc atggttttga tggtgcggaa ttctttgctg ccgccgcctt
    24001 cgaccatata acaggcgagc gtgaacggac tgccggagaa gccgatgagc ggtacgcggc
    24061 cgtccaatgc tttacggatg gaagttaccg cgtcgaaaac gtattgcagt ttttccatat
    24121 cgggaacgtg cagcttggcg atgtcggcct cgtgttgcag ggcgcgtttg aatttcgggc
    24181 cttcgccttc ggcaaaatac agtcccaagc ccattgcgtc agggacggtc aggatgtcgg
    24241 aaaacaaaat cgccgcgtcc aaatcgaaac gttccaaagg ttggatggta acttcggtcg
    24301 ccaattcggt gtttttgcac aaatcgagga agctgcccgc tttcgcgcgt gtggctttgt
    24361 attcgggcag ataacgcccc gcctggcgca tcatccaaat cggcgtgtat tcgacaggtt
    24421 gtttgagcag ggcgcggagg aaagtgtcgt ttttcaaaga gatcatgtgg ggctttcggt
    24481 ttgtttgtaa aacggggaag gaaaacggtt ttcagacaac ctttgcggcg gtttccgcaa
    24541 tgaaaggtcg tctgaaaagg gagggacggg cacatttgcc taagtaatca tcagtagatg
    24601 attaaacggc agcaggtttt catggggttt caatggtgcg tttatcttcg atatggataa
    24661 ctttgggttc ttcagcatca caatcgcgat gagagcgccc ttcgataccg attttgaaat
    24721 cttcatccaa tccttcttct tttaggatta gcgtaaaaat ttcgggatga tggcatgaaa
    24781 cggcatatcc atcgccacta tctaaagtga taatgccaaa atctgcagat ggtgttccta
    24841 tttgaacctt gccccatttg cttaacatct ctttagcttg tttcgccaaa tctttttccg
    24901 gttcaagtaa aatgacgcaa gtcccatttt caaagagcac ccaagatttt tcgtttccat
    24961 taatgctctt tttccaaaca tcaattaact tttggcgata agcaggcgtt ccaatttcaa
    25021 atatttcttt cttttcctta aaggaatctg aagaccccat caaattctcc cagataaaga
    25081 tatttcccaa aatcagctat gctgctctaa cgctgcgtga cgttcgtcat cggagacggc
    25141 ttccaaaacc aagttgcgct gcgcctccgc cttctgcggt tctccgattt cgtcgaaaac
    25201 ctttgctaga accaaacgcg cggaaatact cggctttaat gcaatgctcg cttcaaggta
    25261 gccttttgcc ttgccccaaa gtttgcggcc gtaggcgagc cgaccgagat acatcagcag
    25321 aagcgcgtta tcgggctgtt ctttcagcca agcatcggca aaatcgatgg ctttctgctg
    25381 ttcgcgctcg cccaaaaagc gcacgctttc gacaaaggct tccaaaagct cggggcggcg
    25441 gttgtgcgga taatgctgtt tgacccattt gaccgcatcg gcatacagtc ccaaacgttc
    25501 gtacttttcc gcaaccgata cgctcaattc cccgtttttg aggctgtcgg gaatccgctt
    25561 caggcaggtt ttcaaagcgg cggcatcggc agcatccgcc agctggcggc ggtatgccca
    25621 attttgatac cgttccattt ccgatttgcc caacgcgccc gccttggaaa gtttttcggt
    25681 ttttgccaga acctgcaacg cgtcgcccct gtcgaaagcg taacgaagtt gcagacgcac
    25741 gaggcgcgta aggttggcat tcatcttcgc cgccgcatga agattggctt ccgccgcttc
    25801 gtaatcgcgc cggttcaacg ccgattccgc caacaaaaga taacgggaaa gctgctgttt
    25861 ttccggcagt ttggcgattt ccgcaagata acggtcgcgc agctcgatgt tttccatctg
    25921 tccggcggcg tgcgcgccca gcatcaatgc caaagtccgg ttgtctccgg cctctttgtt
    25981 gaccaacacg cgtgaggctt ctagttccgc cttttcaaaa cgcccttcaa aatacgccaa
    26041 acccgccttg ttcaaggcaa gcgcggcctt gcggccttta cgcgccgaac cgaaacgctg
    26101 catcttttcg gggatattga gtacgccgat aatgaattta aacaagaaat accacaccac
    26161 gacggcaatc agcgaaccta acacaaaggc gtgcaggttg attctgagca tggtctgtcc
    26221 gagtacgata tacacgtcgc cggtgtaaat gcccgaagcc agcgccagtc cgacggcggc
    26281 ggcaaacagg acgacaatcc agactaccgt tttcatgcgc gttctccttt agcggacggc
    26341 gaacgccctg ccttttccgg tacggtctgt ttttttgcag gctgttccgg tttgcgttcc
    26401 gaaggcaggg acggtgcttc tatggtcttc acatccgatg cggcagccgg ttcggaagct
    26461 gttttttcgt ttgccggttc ggaagccgcc tgttccgctt cttgattttc cgccgccgtc
    26521 atgcgcgtac cgtcgcgata ggcgcggacg gcatttaggc tgtttttcaa accgtcatcc
    26581 gcagtcatcc gcacatccaa cgccttcaat tccgccagtt ctttcagcca cgactgcgtg
    26641 gcgggagact tggcatcgaa atactgtctg acggcggctt cggcattgtt caaatcgccc
    26701 tgatagactt cgctgttgcg ctgcattaat gcagtgcgcg catccaaaag gcggaggcgc
    26761 aggttttcac gcacaaaata tgcctgttcg ggagaaatca gcatggcatc gttgttttcc
    26821 aaacgtcgga tttcgaccag ccccttcaat gtgccgaggg atttttccca tacgttctgc
    26881 caccatgaag cggaagcggc ttcgttcttc acctgtacgc ccggtttcag cacgccgtcg
    26941 agcatcagcg gcagtccgga tacggcggtt tccagcctgt cgaggcgcaa tgccgtgccg
    27001 gaaatatcga catagggacg gtttttcagt tccgccaagt cgctgctgac cgcctgcttg
    27061 atcggcagaa gctctgcctg attgaaacgg gacaggcggc tgtcgatatg ctccaatacg
    27121 ccgactgccg tttggatatt gccagtcaac accagctgtt gcgccgccag attgagtatg
    27181 gtctcggttt cgtccaccag ccaatcggcg cgtcctttgg tcaactcgcg ataggatttt
    27241 tgcatttcca agatttgttc gccgtttgct ttgacgtttc cgtccaaacg gtcgagctct
    27301 gattgtatgg cggcttgccg gttgaggttg tctttcaaca gggcggcgtt ttccgactcg
    27361 cccaaggcgg ctttgtcgat tttttggttg aatgccagct cttggttttt caagacattc
    27421 tgtccttgga caaacaaaaa accacttgta cccaaaccca atgccgccaa taccagggcg
    27481 cagactgcca aagcgttgct gccggactgt ttgacgataa aaggagcggc aggaatgtgt
    27541 acttctgttt cgttttcttt gcgtggggaa gaggtttcag acggcatttc ttttgatgcc
    27601 tgtatctcgc gtacgggttc ggatgatttg ttttcaggtt cgcccaccgt tttgctcctt
    27661 attaatttga ggttccggga aagaccatgc cgtctgaaac ggaaatggaa gaatgggaaa
    27721 gcgcggcttc cagcgtaggg acggtttcga ccgaacacac gccttcgcgc ttcaatgcct
    27781 ccgcaatgcg cggatgatgg gtaaagtata gcaaggattt gaagaatcgg gaaaattgcg
    27841 gcggaagctg cccgaacagc aagcgcacca gctcggtcga cgtaatatag gcggcggcaa
    27901 tattttcggt ttggaaattt tgaaagttca aaggtttatg ccgtctgaaa tagacttctg
    27961 ccacctccgt ccgaaaacct ttctcctgca aggcattcat caaaaaatcc cgcccgccgt
    28021 gtccgcgcac aaacaatacg cgcgcacctt cgggcagact gttccaaacc ggcaggcgca
    28081 aaaccgcctc gctgtcgttg ccgtcatcag gcgcgatgac cgttctgacc aaacagcgtt
    28141 ccaatgcgcg gcggctgccc tgccctacgg caatctgcgc ctttatgccg tctgaaaggt
    28201 taaggtacgg gacggcggtt tcaacggcgg tcggactgac ccaaaacacg gcatccgcac
    28261 gggcatattg ttcggacaaa agttccagtc cggcagcatc tgcttcgatt tcgaccggac
    28321 tcaatacttc cgcgcgccaa ccggcattca ggcaagtttc gacatcttct gcggccctgc
    28381 ccgacggacg gacaatcagc atgacgggtt tcgctgtttc catcgcctta tccgccccga
    28441 ttactaggaa aaatcgtcat cgttgaaatt cgcctgcgcg gttttcggcg tacagccgac
    28501 ccgtgcgact tccttgcccc tacgctccac aacgacaccg ctgctttccg tatgagtcac
    28561 ggaatcgaag cctgtccata tggtgtacat aaattcgccg ttttggaatt tcatcgttgc
    28621 ccaacgaccg ctgcccatac cttgccacct gtcggaacgc cccaacaggt cagctttgct
    28681 gttgcgtatg gcaatttctt tttttgccgc actgccgaac gaatattcgt aaagattgcg
    28741 gttgattttt tggacttcta tgtatttgtt gttgtccgtt ttacaggaaa acaccgtatc
    28801 cgcccacacg gtttgggcgg cagccgacag aagcaatacg gcagaaagga acattttttt
    28861 catcgtattt ccttaatggt tgaaaccccg ccactcggac atccgtcctt cgtggcagca
    28921 gcatcagatt ttatttgaaa agggtgtaac cccttccaaa ttagggcaac acatagggcg
    28981 gcgctttatg tgtcgtcctg tgtgttgaaa catcattgcc cgtatcctga aatagaaaag
    29041 aaaatgcccg ttttacggtc ttctttccga caaaatgccg tctgaaacgg atttcagacg
    29101 gcatggtcga aaattattgc gcggaaagcg gcttgagcaa ccaggtttca ccgctttgca
    29161 cgacattttc cagcagatcg atgttcggat gcttgcccgg atgggcacgc gcgtcggcaa
    29221 cgtgttcggc aaaccatatc aaaccctgtt ctgccgcttc tttacccaat tttccgtcaa
    29281 aacgttccgc caaagcattg tacagcttga gcgaaccgag cttaccctga acggcaggaa
    29341 tgtggtgaac cgttttgccg tcggcatcct gaacgtccaa accgcccaga tggtcgattg
    29401 aaggaaatgt ggcgagatag tcttgaaaat tcatatcaac tccgaaaatc agtcagttag
    29461 cgcgtgccga aaatcttgtc gcccgcatcg cccaagccgg ggatgatgta gccgttctcg
    29521 ttcaagtggc tgtcgagcgc ggcggtgtaa atcgtaacgt cagggtgcgc gtcgttgacc
    29581 gccttcacac cctcgggcgc ggcaaccagc accagtgcct tgatattttt gcagcccttg
    29641 gctttcaaaa ggtcgatggt ggcaaccatc gaaccgcctg tcgccagcat aggatcgata
    29701 atcaaagccg gacgttcgtc catactgtcc acaaatttct caaaatagga aataggcttc
    29761 agcgtttctt cgtcgcgctg cagtccgact acactgattt tggcagtcgg aatcaggtcg
    29821 agcacaccgt caagcatacc caaacctgca cgcagtatgg gaacgacggt caatgttttg
    29881 cccttgatgc ggtcgccttc aatctgaccg caccatccgt cgataaggta tttttcgatt
    29941 tcaaaatcac ggcttgcctc gtatgccatc aggcgcgcca gctcggtggc aagcgtccgg
    30001 aatttgtagg tgctgcaatc cgcctccctc atcagggtta atttgtggcg gacgagcgga
    30061 tggttgataa cattaacgtt catcgcggtg ttccaatgtg attttgagac gcgccattat
    30121 actttttttt gatttgccgg aaaagaaaat gccgtctgaa acacgttcag acggcattgc
    30181 cgaatccatc aggctttcag cagctcttgc agctcgcctg cctcatacat ttccatcagg
    30241 atgtccgaac cgccgacaaa ctcgccgttc acataaagtt gggggatggt cggccagtcg
    30301 ctgtattcct taatgccttg gcgcacttcg ggattttcca atacgttgac ggtaacgtaa
    30361 tcggtgcagc ctgccgcgtt caggatttgc acggcgcggg aagagaaacc gcattgcgga
    30421 aactgcttcg tacctttcat aaacaatacg acgcggtgtg tcgttactac ttctttaatt
    30481 tggtcgtgga tggaagccat cgcttattcc ttttgataaa gttgacaaaa tttgtcaggt
    30541 agggcgcatt atacgcaaaa gcggaaactt cccacaatat ttgccgtata tcgcgcagta
    30601 tcaatattgt gaacggaaat attcccactc ctcgacaatc ctgctgtccg gcaaatggca
    30661 cagggcatat ccgccgccgc tttcgtcttt ttttgcttcc cgtctgccgc ctttggcaat
    30721 gcagaatttg gaagcggaat ttgccatgcc tgtccgaaac gtgttggcag gcagccggca
    30781 gcagcgcgga catgcatacg gatgaattgc cttcatggtt ttccttatcg tcggtacagg
    30841 gtcagacggt ccgcatatcg ggtacttttt taaacagccg gtaaaagttt tccgtcgtat
    30901 atgccgcaac ctgttccaat gtttggttcc gcaatttggc gatatgttcg gcggtatggc
    30961 gcacaaaagc cggttcgttc tgcctgccgc gtttgggaac gggggcgagg aacggcgcat
    31021 cggtttccac caaaatgcgg tcgtccggca catatttcgc cgcctcctga accaagggtg
    31081 cgtttttaaa ggtaacgatt cccgagaaag aaatataaag ccccaaatcc attgctgcac
    31141 gggcaaaacc gatgtcttcg gaaaaacagt ggataacgcc cgaattaacc cggcattctt
    31201 tcaggataga caaggtgtcc gccgccgcat cacgcgtatg gacgataacg ggcagtccgg
    31261 tttgattggc tgcttcgatg tggtctgcaa agcgtttgtg ttgccaggac aaatcgcctt
    31321 tgcaccagta ataatccaaa cccgtctcgc cgatgccgac cactttcgga tgggcggcgg
    31381 cttcgaccat ttccgcaatg gaaaattctt cggcttcctt gctgtcggga tgtacgccta
    31441 tggtgcaata gatgtgttcg tgcgcttcgg cgatggcaaa gacttcggag aagctttccc
    31501 tactgacgct gatggcgagt gcctgcccca cgccgtttgc ttccatgttg gacaaaactt
    31561 cgggcaggcg ttctttcaaa ccttcaaaat tgaggtggca gtgcgaatcg atgatatgca
    31621 tggcgttaca tggtaaacgt aggtctgctg ccgccgattg tgttgcccag ttcgacttcg
    31681 atctggtctt tgactttcag gcagttttcg tgttttgtga atgccagccc cacccctttg
    31741 ggtttggagg aagtacgcgc aggattgatc caggcgactt tggtcggcag gaacagtttg
    31801 gggaagttga ggatttctac ggcaagcaga atatcgtccc cgatggaaaa tacgtcgttg
    31861 gtctgcacaa acagaccgcc gtgttccaaa aacggcatgt aggagctgta cagcagattc
    31921 atgtctttca gctgcaacga catcattttt gccggaatat tttgtccgtc tgacataatt
    31981 cacccgttct ctttcttcaa ttccaaatag ttgatgagca gatgctcgat ctgcattttg
    32041 acatttaaag tatgaaaccc gtagggggca agctgtttga gcatatcctc cgccgcaaat
    32101 acattgcgcg gacggaaacc ggataccgtc tgaagcagcc tgtcttcata agcgggataa
    32161 tagacgggtt tcatgtgttg caggcacaat cccaaatcga ccagccattt ctgcatccac
    32221 ccgacaaata cggcgagcgg aagtttttcc ttatcgaaaa gcgcggcgta atccaaaatc
    32281 ttcaacaacc ttggttctgc caaaatatcc aacagtttaa tccgcaacgc acggacaccg
    32341 tccgcctcat caaacagcgg cgctccggaa tggaaagcca gacgttcctc aggttccgcc
    32401 acaccccttt cacgcagata tgccaatgcc tcttcatggg aaggagcggg caaaaccatc
    32461 ttccggcagc gacttttaat ggtcggtaaa accttgtccg ccgcgtggct gaccagcaaa
    32521 aagaccactt gtggcggcgg ttcttccaac actttcaaca aactgttggc ggcttggaca
    32581 ttcatacttt ccgcaggatg aatcagaatc acgcgcaaac cgccccgtac cgaagtcagg
    32641 tacacattat cgatgatttc cctgacggca tcgattttga tctgcaacag tttgcgtccg
    32701 ttttcgggtt cgtccgacaa gggggtgatt tcgtaaaaat cgggatggct tccctgtcca
    32761 aacagatggc aggacataca ttcgccacag ggtttgcagc ccggtgcagg ggtttcgcac
    32821 aacagtgcct tcgccgcaaa gcgggcaaat gtagttttcc ccgtatcttt tttgccggca
    32881 aacagccatg cattgggacg acgctcccaa tgttccgcaa tctgccgcca ttgctcatta
    32941 tgccacggat aaatcatttc aaaccatccg tccggaaatc cataccgcca tcagagcagt
    33001 tccaaatcgg ggctggaact ttgcgcgcgg cggctgcgca actgtaccgc caaacgcaaa
    33061 tcgtgtgcgg aatcggcatt tttcaatgct tcttgcaggg aaatatcgcc tttttcatac
    33121 aattggtaaa ggtgttgatc gaaggtctgc atacccaggg tagtggattt tttcatcact
    33181 tctttgattt catggatgtt gccgttgtga atcaactccg aaatcagggg cgaattgagc
    33241 agcacctcga ctgccgccac cctgcccttg ccgccgtctc gcggaacgag gcgttgcgaa
    33301 ataaacgcct gaaggttgag cgacaaatcc gtcagcaatt gttcgcgccg ctcctcgggg
    33361 aaaaagttga tgatgcggtc gagtgcctga ttggtgctgt tggcgtgcag cgtcgccata
    33421 cacaaatgcc ccgtttcggc aaaggcaatg gcgtagtcca ttgtttcgcg gtcacggatt
    33481 tcgccgataa ggatgacatc aggcgcctga cgcagcgtgt ttttcaacgc cgccatccag
    33541 ttttccgtat ccacgccgac ctcgcgctgg gtgatgatgc agtttttgtg ttcgtggaca
    33601 aactcgatcg ggtcttcgat ggtgatgatg tgtccgaacg aattttcatt gcggtagtcg
    33661 ataagcgagg caagcgaagt cgatttaccc gagccggtgc cgccgacaaa aataaccagc
    33721 ccgcgttttt tcagcgcgac atccttcaag actggcggca ggttcaggct ttcaaacttg
    33781 ggaatcttgc tggtaatcgt acggaatacc aacgccgtcg cgccgcgctg tatcatcgca
    33841 ttgacgcgga agcggctggt gtccggcagg ctgatggcga agttgcactc gttggtcgat
    33901 gaaaattctt ccgcctgctt cgcactcata atcgaaaagg cgatttccat acatttttcc
    33961 gccgtcagcg gttcgtccgt gatgcgggtg attttgccgt ccagcttcat tgcgggcggg
    34021 aaatgggttg tcacgaacag gtcggaacct ttgttttggt tcatatggcg cagccatgcg
    34081 aacagttctt cctttgcggt catattttca tcggtaaaca tcctcgtccc ctaatcttca
    34141 aacaatctgt accttcccat tctaccaaaa acccgccgtg ccgtctgaaa ccgtttcaga
    34201 cggcacggac aaaccacctc cactcaatcc tgccgtgtta gcgtgtataa tcaggcattc
    34261 tatccgaaat tcacaacagg tcagcaatat gtctttgaaa acagtagccg ttatcggcgc
    34321 aatggaacaa gaaatcgagc ttttgcgcga gatgatggaa aatgtcaaag ccgtctcttt
    34381 cggcagattt tccgcctatg aaggcgaatt ggcgggaaaa cgcatggtgc ttgcattgag
    34441 cggcatcggc aaggtcaacg cggcggttgc aacggcttgg attatccgtg aattcgcagc
    34501 ggactgcgtc atcaacaccg gcagcgcggg cggtttgggc aagggtttga aagtcggcga
    34561 cgtagtcatc ggcacggaaa ccgcacacca cgatgtcgac gtaaccgcat tcggctatgc
    34621 ctggggacaa gtgccgcaac tgccggcaag gtttgcttca gacggcattt tgattgaggc
    34681 ggcaaaacgg gcggcgcgga cgtttgaagg cgcggcggta gaacaagggc tgattgtcag
    34741 cggcgaccgc tttgtccaca gcagcgaagg cgtggcggaa atccgcaagc acttccccga
    34801 agtcaaagca gtggaaatgg aagcggcggc aatcgcccaa acctgtcatc agttggaaac
    34861 gcctttcgtc atcatccgcg cggtttccga ttcggcagat gaaaaagcag acatcagctt
    34921 tgacgagttc ttgaaaacgg cggcggcaaa ttccgcaaaa atggtggcag aaatcgtcaa
    34981 atctttataa gacctttatc aaacttatgc caaaacagcg gttttccgtt agaataacgg
    35041 gctatttcca cgttgcaccc gatattcgga cgcaacgccg tttgttcaga cggcatttcc
    35101 caaacccact gtccgacatt caaaacaact ctaatgaaaa atatccgaaa tttctccatc
    35161 attgcccaca tcgaccacgg caaatcgacg cttgccgacc gcttcatcca atactgcggc
    35221 ggtttggatt tgcgcgaaat gagtacgcag gtgctcgatt ccatggacat cgaaaaagag
    35281 cgcggcatta ccatcaaagc gcaaaccgcc gcgctcaact ataaagcacg cgacgggcag
    35341 gtgtatcaac tcaacctgat tgacacgccg ggacacgtcg acttctctta cgaagtttcc
    35401 cgttcgctgt cggcttgcga aggcgcgctt ttggtcgttg acgcgtcgca aggcgtggaa
    35461 gcgcaaaccg tggcgaactg ctacaccgcg attgatttgg gcgtggaagt cgtgccggtt
    35521 ttgaacaaaa tcgacctgcc cgccgccgac cccgaacgcg tggaacagga aatcgaagac
    35581 atcatcggca tcgatgccgt cggcgcggtg caatgttccg ccaaaagcgg catcggcgtg
    35641 gaagacgttt tggaagaaat cgttgccaaa atccccgcac cgaccggcga cgaaaacgcg
    35701 ccgctgcaag cggttatcgt cgattcgtgg tttgacaact atgtcggcgt ggttatgctg
    35761 attcgagtga aaaacggcac catcaaactg aaagacaaag tgcgctttat gagcaccaag
    35821 gcggaaacgc aggtcgagca gctgggcgta ttcacaccga aatcagttca aaaacaagaa
    35881 ctaaaagccg gcgaagtggg ctttttgatt accggcgtaa aagaattggg acaggcgaaa
    35941 gtcggcgata cggttacttt ggttgccaac cctgccactg agccgctgcc cggtttccaa
    36001 gaggtgcaaa gccaggtatt cgccggactt tatcccgtag aaagccacga ctacgaagct
    36061 ttgcgcgatg ctttggaaaa attgcagctt aacgatgctt cgttgaaatt tgagcctgag
    36121 gtttcccaag cattgggttt cggcttccgt tgcggcttct tgggtctgtt gcacttggaa
    36181 atcgtgcagg aacgcttgga gcgcgagttc gatatggatt tgattaccac cgcgccgacg
    36241 gtgatttatg aagtcgtgtt gaaaagcggc gaaaaaatcg aagtcgaaaa tccgtccaaa
    36301 ctgcccgaca tcggcagcat cgaaaccata ctcgagccga ttattaccgc gaccatcctt
    36361 gtgccgcagg aatacgtcgg caacgtcatg actttgtgta accaaaagcg cggcgtgcag
    36421 gtcaatatgc agtacatggg ccgccaagtg atgctgactt acgatttgcc catgaacgaa
    36481 gtggtgatgg actttttcga caaactcaaa tccacttcgc gcggctatgc ttcgttggat
    36541 tatcatttca aagagttcca accgtctgat ttgattaagc tggatattat ggtcaacggc
    36601 gaaaaagtcg atgccttaag cctgattgtg caccgtcaaa gcgcggttca ccgaggccgc
    36661 gagctagcat cgaaaatgcg cgagctgatt ccgcgccaaa tgttcgacat tgccgtccaa
    36721 gccgccatcg gcagccagat tatcgcccgc gaaaacgtca aagcactgcg taaaaacgtc
    36781 ttggcgaaat gttacggcgg cgatattacg cgtaagaaaa aactgcttga aaaacaaaag
    36841 gcaggtaaac gccgtatgaa acaagtgggc aatgtggaaa ttccgcaaag cgcgttcttg
    36901 gcgattttgc aggtgagcga caaataactc cggcacacag ggcggcgcgt cgggaacacc
    36961 cctcaaaccg ccctgatttc aggggcacgc gcccgcaaac aaattttgca atttgccgcc
    37021 ttaaaacggt tgcacggaca ggcgggtttt taagcattaa ggaacaacaa tgaacacaat
    37081 gctaatgtcg ggcgcggctg ccgcgctgct tgccggcatc atcctttatt tcaaaagcga
    37141 caagaagcgg caggaaaacg gggaatggag ttccggcctt gaatacgcct atatcctgac
    37201 agcggtcggc gtgtttgccg ctttgtccct gtttatgagc tttaccgccg ttttcctgat
    37261 tttcgttgta ttgtgcggta cggcttgggg ggtatataaa taccgcctga agactcatcc
    37321 cgaaatctcg gaaagcagcc acttcggcga ttatttcggc agtttcttcc ctaccgtttt
    37381 ggtattgttc ctcatccggt cgtttatcgc cgaaccgttc caaatcccgt ccagctcgat
    37441 gcgcccgggc ctgatcaagg gcgatttcat tttggtcggc aaattttcct acggcctgcg
    37501 cgtacccgtt ttaaacaata tatttattcc tacaggcaaa atcgaacggg gcgatgtcgt
    37561 tgtttttaat tatcctctgc agccggagat gacctacatc aagcgtattg tcggcattcc
    37621 gggcgatgtg gtcgaatatc gggataagat tttgacggta aatggcaaac ccacttccga
    37681 cattcctgac ggcacatacc gttatcccga cgacaccgac ccttccgaaa tccacaacac
    37741 ggatatgttc cgcagcggtc tagacggcaa atccttcaat attctgaaaa aagaaggaca
    37801 gcctgccgtt tccctgcccg tattgggcaa atatacctcc gatattatgt ctgaaaacgg
    37861 atattccata gagcaaagcg gtttggaaca ctgccaatat gccgacgacg gcagcggttt
    37921 cgtgtgcaaa gttcccgaag gacgctattt cgctatgggc gacaaccgcg acaacagtgc
    37981 cgattcgcgc tactggggat ttgtggatga caagctggtt gtcggcaagg caatgttcat
    38041 tttgatgaac ttcggcgatt tcggcaggtc cggtacggca atccgttagg ccaggaagcc
    38101 aagaatgccg tctgaaaata gtttcagacg gcatttgcta ttcctcactt ttacaggcga
    38161 gtctaacgtt gtcggatttt cctacctatc agtccaatat acgcaggttt tgcacggcga
    38221 actgccaata ttgccctgat gaaatcaatg tgaagactgc cgtttcagcg aaccgaggta
    38281 aattgccgaa agcgtcagcg ccacccctgc ccactgcacg ccttcaatcg gtttgcccaa
    38341 cccgaaataa tcgatgaaca gggcggcaac cggttcggac aaaagcagca gccccgtcag
    38401 cgacagcgaa agcagcggaa tcgcataggc aaccatcgcc cacgcgaagc actgcatcac
    38461 cacgccgtac acaagcacca aacccgcatc tttccaagtc gtcggataaa gcgcgccgcc
    38521 atccatcagc aatgccggaa caaccagcga taccgcgccg cccaaactca aaatcatcat
    38581 tgaagggaaa agcgccaccg gctcgatttc atgggttttg cggacaaaca ccatcgacag
    38641 ggcgagcatc agtcccgata ccaaaccgct ggcgaatccc caaaccgcat taccgttgta
    38701 gccgaattcc gcaccggcaa tcatcgccac gccggcaact gccgatatta agcctgcctt
    38761 tttcagcccg ctcaaacgct cgccgaaaaa gaaaacaccg attgccgaca agaaaaagat
    38821 ttgcaggctg ttgagcaggg tggaaatacc cggcccgacc gcgtgtatgc tttcgtgcca
    38881 caacgccaaa tcgaaagcga gaaacacgcc cgccgtcagg gcatatcgga cggttttcct
    38941 gttttttggg aatttttgcc tgaaaaaccg tgctaaaaac caaaatacga acaccgaaat
    39001 cagcaaccgc caaaatgcga ttgcatacga accgacgggg acggatctga caatcagact
    39061 gcccagaccg aacaccacgc aacccagcac cagcaatggt gcggcatatt ttttgccgtt
    39121 accgaccaca atatcccctc ccttccccat acggctgccg cgccgacggc aagaatgccg
    39181 ccattccgaa aatatgccgt ctgaagccca aagccccggc tgcacccgga caaacggctg
    39241 attttacggg caggcaggct attttccgtt ggggcaaaaa accgtcctgc caaataaaac
    39301 cc
//
LOCUS       DAWGRZ010000003        31903 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000003 DAWGRZ010000000
VERSION     DAWGRZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31903
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.003"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(177..824)
                     /locus_tag="WHT44_000089"
     CDS             complement(177..824)
                     /locus_tag="WHT44_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ2532333.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(821..1189)
                     /locus_tag="WHT44_000090"
     CDS             complement(821..1189)
                     /locus_tag="WHT44_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ2532334.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(1199..1870)
                     /locus_tag="WHT44_000091"
     CDS             complement(1199..1870)
                     /locus_tag="WHT44_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ2532335.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVEKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(2035..2754)
                     /locus_tag="WHT44_000092"
     CDS             complement(2035..2754)
                     /locus_tag="WHT44_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ2532336.1"
                     /translation="MATLSDKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGYPEPFAICFDLISAEEKEFEHFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            complement(2837..4150)
                     /locus_tag="WHT44_000093"
     CDS             complement(2837..4150)
                     /locus_tag="WHT44_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEZ2532337.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALADLQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFASAEACLQSLSDAV"
     gene            complement(4548..5420)
                     /gene="hpnD"
                     /locus_tag="WHT44_000094"
     CDS             complement(4548..5420)
                     /gene="hpnD"
                     /locus_tag="WHT44_000094"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ2532338.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHRVAGVVGCLIARILGFSDDQTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMRRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            5591..7453
                     /gene="hscA"
                     /locus_tag="WHT44_000095"
     CDS             5591..7453
                     /gene="hscA"
                     /locus_tag="WHT44_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ2532339.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVA
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDDTITQMLKDSMSNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEELQQI
                     RQGIADLQGRLKDGKAEDIRAAAAKLGSITDNFAAKRMNRNIQRALTGQSVDNI"
     gene            7530..8186
                     /locus_tag="WHT44_000096"
     CDS             7530..8186
                     /locus_tag="WHT44_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ2532340.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISDVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKN
                     ETAESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSK
                     IRLFKEAYFGKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPQ
                     "
     gene            8223..8984
                     /locus_tag="WHT44_000097"
     CDS             8223..8984
                     /locus_tag="WHT44_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ2532341.1"
                     /translation="MMGDSVIYYVEQADEPVNRADERARKTFKYFWRELFWERRRIIS
                     ALDFAMVKVPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESVCV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQILLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNSDYLREQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASTTSEGYTALDFACLTGWQNVADLLEPRH"
     gene            9039..9380
                     /gene="fdx"
                     /locus_tag="WHT44_000098"
     CDS             9039..9380
                     /gene="fdx"
                     /locus_tag="WHT44_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ2532342.1"
                     /translation="MPKITVLPHTTLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(9803..10357)
                     /locus_tag="WHT44_000099"
     CDS             complement(9803..10357)
                     /locus_tag="WHT44_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ2532343.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRDLIALDKAAAGCDSAMLRAQRWLIYTQIAERLAGSGGGFT
                     VTVESVSAACPELEGRYLELVRRAAVSFGFTQGWENPAGGQEKTQAVPDAASGHSVRK
                     PIKINIEARVERYPAYCPTVALKV"
     gene            10570..10767
                     /gene="iscX"
                     /locus_tag="WHT44_000100"
     CDS             10570..10767
                     /gene="iscX"
                     /locus_tag="WHT44_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ2532344.1"
                     /translation="MKWTDTQRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(10835..11236)
                     /locus_tag="WHT44_000101"
     CDS             complement(10835..11236)
                     /locus_tag="WHT44_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ2532345.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTATLREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            11448..12407
                     /locus_tag="WHT44_000102"
     CDS             11448..12407
                     /locus_tag="WHT44_000102"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ2532346.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            12475..13785
                     /gene="tilS"
                     /locus_tag="WHT44_000103"
     CDS             12475..13785
                     /gene="tilS"
                     /locus_tag="WHT44_000103"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ2532347.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNTDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRLEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAASDTLAMGGIHKDVKKILQGKRVLPV
                     LRPIWPLVADSGNRPLALANCCADFQYSVSDGILPVHPDFPILF"
     gene            13859..14644
                     /locus_tag="WHT44_000104"
     CDS             13859..14644
                     /locus_tag="WHT44_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ2532348.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGMPVGVYIPEAKMPSEEVRKLTAVLPEDGFFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGIGAVILGKGCADAWSPKVLRAGM
                     GAHFLSEIYPQADLEIWLVRYKGRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            14654..14794
                     /locus_tag="WHT44_000105"
     CDS             14654..14794
                     /locus_tag="WHT44_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532349.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LI"
     gene            14779..14931
                     /locus_tag="WHT44_000106"
     CDS             14779..14931
                     /locus_tag="WHT44_000106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532350.1"
                     /translation="METHLIVQCRGGGGFGLGVVEMVLTDIAQNRANGIRTNKEFGKK
                     FVKSTN"
     gene            15121..15465
                     /locus_tag="WHT44_000107"
     CDS             15121..15465
                     /locus_tag="WHT44_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532351.1"
                     /translation="MRIFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLSCYY
                     DEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKELDEV
                     LLKYIPFFEEER"
     gene            15477..15782
                     /locus_tag="WHT44_000108"
     CDS             15477..15782
                     /locus_tag="WHT44_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532352.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPKGNAQKDGSAAGIKPH"
     gene            15844..17220
                     /gene="mpl"
                     /locus_tag="WHT44_000109"
     CDS             15844..17220
                     /gene="mpl"
                     /locus_tag="WHT44_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ2532353.1"
                     /translation="MKHIHIIGIGGTFMGGLAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIDVYEGFDAAQLDEFKADVYVIGNVAKRGMDVVEAILNLGLPYISGPQWLSENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHYL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHVGVDIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKSALPVSLKEADQ
                     VFCYAGGVDWDVAEALAPLGGRLNVGKDFDAFVAEIVKNAEVGDHILVMSNGGFGGIH
                     GKLLEALR"
     gene            17427..18479
                     /gene="bioB"
                     /locus_tag="WHT44_000110"
     CDS             17427..18479
                     /gene="bioB"
                     /locus_tag="WHT44_000110"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ2532354.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRQHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKVDY"
     gene            complement(18557..19192)
                     /locus_tag="WHT44_000111"
     CDS             complement(18557..19192)
                     /locus_tag="WHT44_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ2532355.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     RKRITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGRFVS
                     GNIIPITQVGDKIPKIDPEKTVIGRIIYLNRSDFPKGNGLDVSPDGSITRR"
     gene            19515..19775
                     /locus_tag="WHT44_000112"
     CDS             19515..19775
                     /locus_tag="WHT44_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ2532356.1"
                     /translation="MTKAVRRKNVQTPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(19838..21697)
                     /gene="ilvD"
                     /locus_tag="WHT44_000113"
     CDS             complement(19838..21697)
                     /gene="ilvD"
                     /locus_tag="WHT44_000113"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ2532357.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEQWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(21870..23639)
                     /gene="cysI"
                     /locus_tag="WHT44_000114"
     CDS             complement(21870..23639)
                     /gene="cysI"
                     /locus_tag="WHT44_000114"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ2532358.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPVRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEEFARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(23666..25480)
                     /locus_tag="WHT44_000115"
     CDS             complement(23666..25480)
                     /locus_tag="WHT44_000115"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ2532359.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVSRAELKD
                     YKAKNIAGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCQAGKDFDRRFEELGAKRLLERVDADLDFTASANAWTDNIAALLKEEAAKNRATP
                     APQTTPPAGLQTAPDGRYCKAAPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKMMPVARALSSHFELTQNTPAFV
                     KGYAAFAHYEELDKIIADNAVLQDFVQNTPIVDVLHRFPASLTAEQFIRLLRPLAPRL
                     YSISSAQAEVGDEVHLTVGVVRFEHEGRARTGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFARDFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(25477..26763)
                     /locus_tag="WHT44_000116"
     CDS             complement(25477..26763)
                     /locus_tag="WHT44_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ2532360.1"
                     /translation="MTAQHQTPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKEAIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDAHFVPMSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ETLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(26802..27725)
                     /gene="cysD"
                     /locus_tag="WHT44_000117"
     CDS             complement(26802..27725)
                     /gene="cysD"
                     /locus_tag="WHT44_000117"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ2532361.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(27763..28503)
                     /locus_tag="WHT44_000118"
     CDS             complement(27763..28503)
                     /locus_tag="WHT44_000118"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ2532362.1"
                     /translation="METTLFKPALWQIPHIGSGGETALAEKTETLKQRLHRIVGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRF
                     RPVREDADRYVESKGRFAFYDSVEARRECCRIRKTEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            complement(28514..29965)
                     /gene="cobA"
                     /locus_tag="WHT44_000119"
     CDS             complement(28514..29965)
                     /gene="cobA"
                     /locus_tag="WHT44_000119"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ2532363.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKGKLKSVTERRRFWEKQFNGRFAALVKNRQNTLAERELAGQLEQSRQNDQGGS
                     VSLVGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGE
                     RTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAA
                     VGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALI
                     AERLQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLH
                     EKLAWFGENGEGENRVGQTYPALGGLNAGQRAA"
     gene            complement(30091..30249)
                     /locus_tag="WHT44_000120"
     CDS             complement(30091..30249)
                     /locus_tag="WHT44_000120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532364.1"
                     /translation="MPSEALFALRTASPHHANGLNARNGFRPFFFRNRYKTENRRIRS
                     RPHIAFSF"
     gene            complement(30262..30918)
                     /locus_tag="WHT44_000121"
     CDS             complement(30262..30918)
                     /locus_tag="WHT44_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ2532365.1"
                     /translation="MKNKTKVWDLPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LFVLFLLVFRLCWGIWGSDTARFSRFVQGWAGIRGYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLILPMITGFKYIEGKTSIRFAGKAALAAALSVASLAAAAILLLS
                     "
     gene            30982..31872
                     /locus_tag="WHT44_000122"
     CDS             30982..31872
                     /locus_tag="WHT44_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ2532366.1"
                     /translation="MFPVFHLSGESRRQMLQTALRFPHVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDTLPFAVIAGFPEIMLDTADSLAKRQD
                     INAWVVGCGLGTGRAAVGTLAGILTEHTDKPVVLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITKSM"
BASE COUNT         6876 a         8383 c         9260 g         7384 t
ORIGIN      
        1 acaaccacgc cggaattaag tttaaatttg aataaaaggt tcgggttctg caaaatacag
       61 aacccgaacc ttgttcggat attgaaaccg gctgcccgat tttgggcggt gcggcttgca
      121 agtatcaaga ttcgcatatg ccgtctgaag ctcggagagg ttcagacggc atatgcttat
      181 ttgggctgct cttcaacgaa tctcggacct ttcaagatgc cgttgtgaga atagggcgac
      241 agcaggttgt atgcggcggt tttggaaacc tgataaccgc ggtcggtcag gctgttggca
      301 atctgattga ccactgcgct gaccaaagcc cccaacaggc cgctgttgct gttgttgctg
      361 ccttcgcgga tgctggccga acccgaccac aactcttttc cgttgcggga atcgaccagc
      421 cgtgctttgg cggatacggt cgtcacgctg tctaaaattt gatatgaagt gccgtattcg
      481 gtaaccgtaa tgtacaaaac cgcatcattg ccgaaaatct gatgcagttt ttccggccgg
      541 acggcgtgaa tatcggcggc attggtcaag ccgttttgtt tgaaggtttc ctccacgact
      601 gcggcgggga agacgtaata gccggcttcg gaaagcggcg cggcggtcga agccagtaca
      661 ccccatgttc cgttgacatc gggcgattcg ttcagcggcg gaaccaccaa aattgaagcc
      721 ggtttgcttt ccttgaatga cgtgtagtcg aaatcgggcg ctttttgaac ttggcaggca
      781 gacagcgcca acacggcggc aagccctaaa atcaaaggtt tcatcgcttg cctcctttac
      841 cggttttcat caggaagtcc ataaatacgc ccgattcggg aaacagcctt ttctcttctt
      901 caaactggcg gaacgcgccc tctttgtctc ccgaacggga aagcagcagt cccagatggg
      961 cgtgcgcacc cggggcggca ttcatttttt tgttgccggc ttccacaaag tatttttcca
     1021 tcttttcggt ctgcttgccc aacgaagtgt cgtcgttttt caaaccttca tagacggtat
     1081 cgggatagcc gccgtaataa tacagggatt tttgcccgtt gccgccgcag gcggtcagag
     1141 ccaagaccgc cgcacacagc gacaaacggc tcaaggtttt cggattcatc atttctcctt
     1201 aacggttggg ttgccatgcg ccgttgtcaa cagcctgaac caggctgttg acggcttcgc
     1261 ggattgccaa gtctaaaact ttgccgttca aagtcgcatc gtagccggaa gtgccgccga
     1321 aaccgatgat ttcacggttg gaaagtgcgt attcgcccgc gccctgtgcg gaatagacga
     1381 tttcggaagt attgacgttg acgatattca gagccacttt tgcataggcg atttgcgatt
     1441 tgccgcgacc caaaatgccg aagagctgat gatcgccgac atctctgcgt ccgaattcgg
     1501 ttacatcgcc ggtaacgaca taatctgcgc ctttcaggtt atgcgctttg ccggaaatgc
     1561 cggattcctg ttttaatgcg ttcaaattgg tgcggttcag tacgttgaag cggttggtct
     1621 gttgcaggtg cgttactaga atggtttttg cctggctgcc caaacggtct tccccgtcgg
     1681 agaaaatgcc tttttggaag ctggagcggt tgtcgaatgt tccgacggaa atcggggtac
     1741 gaacaccgtg atattgcgta ttgtaggagg cgactttctc tacctcgaga ctgcgtgagg
     1801 attcggtcgc acagccggtc agtgaaacgg cagcggcggc aaggacaacg gcggtggaaa
     1861 cggttttcat aaaatttacc ctaaggtcaa gttaaggaaa taacgggttg tcattattgt
     1921 ccttatgtaa atttaagtca aggtgtttgt ctgtgcggga cggatgcgcg cggaaggtac
     1981 ggatattttt caaacggaat cgcggcgcgg gcgggatgcc tgccttttcc ggatttagag
     2041 gtaaacgatt tcgccactcc gccctttgct ttcggcactt gcccaccaga caaatgcggg
     2101 cagcacgtcc ccgtagcttt tgcgttcgct tttggcttcg cccggatggg atttgatgcg
     2161 ttgcggggaa ttgatggggc cggggacgag gacgttggcg cgcaggttgc cgaagcgttc
     2221 ccattcgtcg gcggcgactt tgcacaggta gttcaacgcg gctttggacg cgccgaagcc
     2281 gccccagtag gctttgggtg tttcgccgtg gctttcgccg acgaagatga cggacgcgtc
     2341 gggcgactgc ttcagcagcg ggaacagggc gcgggtcagc cccataggtg cgacggtgtt
     2401 gatgcggtat tggttgaccc attcggcgac ggtttggaaa tccagcggcg agagggcgta
     2461 aaaatagccg gcgcagtgga cgatgccgtc cagtttgcct tgcgtggctt cggcaatggt
     2521 ggcggcgaaa tgttcaaatt ctttttcttc cgcgctaata aggtcaaagc agatggcgaa
     2581 tggttcgggg tatccggctt cgacaatcgc gtcatacact ttttccagtt ttttctgatg
     2641 acgggcaacc aaaatcacgg ttgcgcctgc cgccgcatag gctttggcga cctgttcgcc
     2701 cagaccttgc gatgcgccgg ttactaagat ggttttgtcg gacagtgtcg ccatactttt
     2761 tcctttttgg ttgtcggtta aggtatttta gcgttttgcc gcaccttgta aagcgtcccg
     2821 atgtcggccg gcgttttcag acggcatcgc tcaggctttg caggcaggct tccgccgacg
     2881 cgaaacctga ttgtacggcg gcttcgagcg tggcggggta gtccgggtgg aggtagtcgc
     2941 cggcggggaa gatgcggtgc cggtgcaacc acgacaagtc cggcggcggg gcatcggctg
     3001 cggttgtggc gcgtttttcg gtgatgacgc gcacggcttc gggttcgccc aaatgcggaa
     3061 ggatgcgttt gaggtcggcg tgggctttgt ccgcccacgc ccggtttgca aacgcgccga
     3121 cgcggtcgga aacgctgatg acggcggaca cttcgttttc aggcagtccg agcctgcccc
     3181 ggcaaagcag ccattgcacc gtgccgtcgg caaggccggt cagcggggcg ggcaggcgga
     3241 cgggttcggc gtagcgcaga tagacggtgg tgatggcgtg gtagcgaagg ttttgatatg
     3301 ccgtctgaac gtgttcgggc gtgccttcgg gcaggagcgc ggcggcgtgg tagggcgcgg
     3361 tggcggggac ggcggcatcg aaagcttcgc cgttgacgag cactttcccg tccgggaggg
     3421 tgttcagacg gcatacgcgc gtttcgaggc ggatgtccgc gccgagccgt tgaagatccg
     3481 ccaaggcggg ttcggcgacg attgcgccca aatcctgctt gggtaggaga tagtcgctgc
     3541 cggatttttt cgtcagcacg ccgtcggaca aaacgttgca caacacgcgc aggcttgcgg
     3601 tttccaaagg cgtgttgagc gcgccccaaa ccaagggctg ccaaaactgc atcacggcgg
     3661 cacgcggcac gttccgctgt ttcagccatt gcgccactgt cgtgtcgggc tgtccgaggc
     3721 gtgcggactt ctgcaaatcg gacatatcgg caagcagttt ggctttgaat gcagtcggtg
     3781 cacgccgggc aagcagcacg ccgcccaaaa tatgcagcgg cgcgggcagg gggagggcgc
     3841 ggaactgcaa accgccgtgc atatgccagt gcagcggtac gcgcaaaaag gcggcacggg
     3901 gatccgaacc gatggttttc atcaggcgca acacgccccg gtatgcgccg agcaaaatgt
     3961 gctgcccgtt gtccaaaaaa ccgaaaccgt cggtatttcc ggccagtgtg cgcgccctgc
     4021 cgcccgcctg ccggccggct tcaaacaggg taacgtcggc gtgccgcgcc aaggtgacgg
     4081 cggcggacag tcctgcccag cctgcgccga tgacggcgat tttcgggcgc ggatgcggcg
     4141 tgttcatcat ttattcctcc aatggttttg cagccgtatc tatttccgtt tccgaaaatg
     4201 acggtagaaa aggatacagg ctaaaaataa aggcagcaga aagcgcgcat agggatacca
     4261 cggatggtct gcatgccagt atccgtaccg tccctgtgga acggtatcat agcggtaact
     4321 gtcggggaaa aagttgatcc aaaccgttac tgccagccat aaggctatat ccctgactgc
     4381 tttcatttac ttctgctcct gtttaaattc ccagcaattc catttcaaag cgcgaacgcc
     4441 aacgggattg cgcggttacg atgcagactt tcagacgatg gttgaaaccc cgtccggcgg
     4501 ggctgtggga atcgggtttg cctgacgggc ggcgggcggt atgcgcctta tgcccgttcc
     4561 ggcgtgccgg ggcgcggttt gaatccgaat aaccaggttt tcagggcaat gcgttttttg
     4621 cgcggcgaag ggagggcgat tttgtatttg aggacgtttt gtgcgccgtc tcggtcgatt
     4681 tcgttcaata gctcgtaata aaccgccgcc atgaccagtc cgactttttg ggctttttta
     4741 tcggcatcag gcagcagcga tacggcttcg cggtaggttt cgcgggcgcg tttgatttgg
     4801 aacgccatca attcggcaaa attgcccgtc gggctgcatt gcaaaatcac gcttgcgggt
     4861 acgtcaaacc gccgcatttc ctccatcggc aggtaaatcc gccccctgcg cgcgtcttcg
     4921 ccgacatcgc ggatgatgtt ggtcagttgc agcgcaagtc ccatcttgtc ggcgtattcc
     4981 agcgtttggt cgtctgaaaa ccccaaaatc cgcgcaatca ggcagccgac cacgcctgcg
     5041 acgcggtggc aatacagttt caattcttca aaactgccgt aacgggcttg aaccaaatcc
     5101 atctgcatcc cgtcgattaa ggcttccagt tcatatttcg gcagcttgaa ggtttcctta
     5161 acttgccgca aggcctgatt gacggggtgt tccggcatcg cgccgccgaa taccttgtcc
     5221 aaatcgccgc gccaccagtt caatgtcgcc tgtgcaacat cggggttgga acattcgtca
     5281 accacatcgt ccaattcgcg gcaaaaagca tataaaaccg ttaccgcatc ccgtttttcc
     5341 tgagtcagga aacggaagcc cgacaaaaaa ctggagcggc tttcttctgc tttttggcgg
     5401 caatagtcga gtcctttcac gatttatatt cctaatgatg ggcgggaaag gcggatttta
     5461 tcggcatttg gcggtagagg gcaatttcgg cggcacgacc taatccttag cggtttgctc
     5521 aactatcggc gcaaattctg ttaaaatgcc gctttccttc ctttacacac cgcaccgaca
     5581 ggcagaattt atggctcttt tgcagatttc agaaccgggt atgtccgccg ccccgcaccg
     5641 gcaccgtttg gcggcaggca tcgatttggg tacgaccaac agcttggtcg ccaccgtccg
     5701 cagcggcagt gccgcctgcc tgcccgatgc cgaagggcgc gttaccctgc cttccgtcgt
     5761 ccgctatctg gaaaacggcg gcattgaagt cggcaaaacc gccctgtccg cccaaaaaac
     5821 cgacccgctg aacaccgtca gctccgccaa acgccttatc gggcggacgc ttgccgatct
     5881 gcatcaaaat acgcactacc tgccttaccg tttcggcgac aatcaacgcg ttatcgaact
     5941 gcatacgcgg cagggggtga aaacgcctgt cgaagtgtcg gcggaaatcc tcaaaaccct
     6001 taaatcgcgc gccgaagaaa ccttgggcgg cgatttggtc ggcgtggtga ttaccgtccc
     6061 cgcctatttc gacgatgccc aacgccaggc caccaaagat gccgcgcgtc tggcgggttt
     6121 gaacgtattg cgcctgctca acgaacccac cgccgccgca atcgcctacg ggctggacaa
     6181 cgcctcggaa ggcacgtttg tcgtgtacga cttagggggc ggcacattcg acgtatccgt
     6241 attgcaactg accaaaggac tgtttgaagt caaagccacc ggcggcaaca gcgcgttggg
     6301 cggcgacgat ttcgaccacc gcctgttctg ccgcctgctc gaacaaaacg gactctccca
     6361 actcaacgaa caagacagcc aactcctgct ctcgctcgtc cgcgccgcca aagaacaatt
     6421 aaccacgcaa accgaagcgc gcattcaggc gacgctttca gacggcatgg caatcgacac
     6481 aagcatcagt cgcgccgagt tccacaacct gacgcagcat ttggtgatga aaacgctcga
     6541 accggtcaca caggcgttga aagatgccgg tgtcggtaaa aacgaagtca aaggcgtgat
     6601 tatggtcggc ggttcgaccc gtatgctgca cgtccaacag gcagtcgcca ccttcttcgg
     6661 acaaaccccg ctgaacaacc tcaaccccga cgaagtcgtc gcgctcggcg ccgccataca
     6721 ggcaaacgtc ctcgcaggca acaaaaccga cggcgaatgg ctgctgctgg acgttacgcc
     6781 cttgtcgctc ggtttggaaa cctacggcgg cttggcggaa aaaatcatcc cgcgcaattc
     6841 caccatcccc accgcgcgcg cgcaggactt taccaccttc aaagacggtc agaccgcgat
     6901 gacgatacac gtcgtacaag gcgaacgcga actggttgcc gactgccgca gccttgccaa
     6961 attcaccctg cgcggcattc cgcctatggc ggcgggtgcg gcgcgtatcc gcgtaacctt
     7021 ccaaatcgat gcggatgggc tgctgtccgt ttccgcccaa gaacaaagca ccggcgtaca
     7081 ggcgcaaatc gaagtcaaac cctcctacgg cttggacgac gacaccatca cccaaatgct
     7141 caaagacagc atgagcaatg ccgccgaaga tatggcggca cgcgcccgtg ccgaagccgt
     7201 agtcgaagcc gaaagcctga ccgatgccgt caacgccgcc ctcgagttgg acagcgattt
     7261 gctggatgcc gaagaattgc aacagattcg gcaaggcatc gccgatttgc aaggccgtct
     7321 gaaagacgga aaagccgaag acatccgtgc cgccgccgcc aaactcggca gcatcaccga
     7381 caatttcgcc gccaaacgca tgaaccgcaa catccaacgc gcgctgacag gccagagtgt
     7441 cgataatatt tgatacttaa acggtttcag acggcataga aataatccga tgccgtctga
     7501 aggctcgaaa acacttgaaa aacatcgata tggaaaagtc aggcattgtc tatctgatga
     7561 aaaccgtcat caagggcgtg tataaaatcg gcatttcgga tgtaagcaat tttgaaggca
     7621 gaatgcgcca tttggaaaac aacggttatg cgaacgttgc cggattggaa cgcatcctcg
     7681 ccgtcaaaac cgacaattac aaagaaaaag aaaacctgct ccatgaaatt ttcagcaaaa
     7741 gcaggatagg cgataccgaa ttgttcgccg tggacgaaaa ccttgtgaaa cgtttgtttt
     7801 tatcgcttcg cggcgaaatc gtgttcccga aaaacgaaac ggcggaatcg gaatttgaaa
     7861 aaagcgtcca cgaacgcagg caggaaggga atgccgggtc aggccgcaaa caactgcttg
     7921 atttggtacg gcgcggacac cgggaatacc cttacgcgct gccccggctt ttggcgggcg
     7981 cggcatccta caagccgaaa aaatcgaaaa tccgcctttt taaagaagca tatttcggca
     8041 aaagcggcac gaggctgacc gacgaaattg cagacggcat ccatatttac acctgttttt
     8101 cgcgggcgga tttggaaaaa gcctattccg aatatttgga acttttcaaa tccgaatcgg
     8161 atgccgaagg cagaaagccg cagtaaggtg caaacagata ccgtacacgt tgaggagcag
     8221 atatgatggg cgattccgtc atttattatg tagaacaggc agacgaaccg gtaaaccgtg
     8281 ccgacgaacg cgcccgtaaa acattcaaat atttttggcg cgagcttttt tgggaacgcc
     8341 gccgcattat ttccgccttg gattttgcca tggtcaaagt cccttttttc caagacggcg
     8401 aagacggcga aatttgcgaa catatgtgga tcgacgatat ttattttgac ggtctttata
     8461 tttacggtgt gctgaacaat gaacccggcg aactgaccaa tgtcgaacaa ggcgaaagcg
     8521 tttgcgttcc ggttgacgac atcagcgact ggatgttcgt gtgcaacggc atcccctacg
     8581 gcggctttac catacaggca atgcgcgggc agatgacgga agaggagcgc accgaacacg
     8641 atgccgcatg gggaatcgat ttcggcgatc ccgggcagat attgctggtg tatgaagaaa
     8701 aagaacatcc cgaaaatctg gaagagcatc cgatgtgccg gaactgtatt gacgattttc
     8761 ggcaacagtt gtcccaaaac tcggattatc tgcgggaaca ggacgaagac ggctatacgc
     8821 cgcttcatca tgaagccatc gcaggaaatg cacttatggt tcaagccatg cttgaatacg
     8881 gcgcaaatcc tgcctcaacg acatcggaag gctataccgc cctcgatttt gcctgcctga
     8941 cgggctggca aaatgttgcc gacctgctcg aaccgcgaca ttaggcagac agttttccga
     9001 aaacgaacac aaacactttt tacagaaaga caataaaaat gcccaaaatc accgtacttc
     9061 cacacacgac attatgcccc gagggtgcag tcatcgataa cgcacccgaa ggtaaaaccg
     9121 tccttgacgt gctgctcgac catgatatcg aagtcgatca cgcctgcgaa aaatcctgcg
     9181 cctgcacaac ctgccacgtg attatccgca aaggtttcga cagcctagaa gagccgaccg
     9241 aattggaaga agacctgctc gatcaggctt ggggtttgga agccgattcg cgcctgagtt
     9301 gtcaggcggt tgtcgccggc gaggatttga ttgtggaaat ccccaaatac accatcaacc
     9361 acgcgcgcga agaacactga aaacaggccg tctgaagccg gcacgcttca gacggcattg
     9421 ttgcgcggat aaggcgcaat cgcccgaaaa caggcgttcg tacaggcgga actttcgatt
     9481 ctatagtgaa ttaacaaaaa tcaggacaag gcggcgagcc gcagacagta cagatagtac
     9541 ggcaaggcga ggtaacgctg taccggttta aatttaattc actatatatt gatttttatc
     9601 ggttttctga cggaataatc cagtgcggca tccgaggcgg attactcgga cgcgatgcac
     9661 cggtatttat cggttttgca gccggaaaaa ccgccggcgg gttatagtgg attaaattta
     9721 aaccagtaca gcgttgcctc gccttgccgt actatttgta ctgtctgcgg ctcgccgcct
     9781 tgtcctgatt tttgttaatc cactatactt ttagggcgac ggtcgggcag tatgccggat
     9841 agcgttccac tctcgcttct atattgattt tgattggttt tctgacggaa tgacccgatg
     9901 cggcatccgg gacggcttgt gttttttcct gcccgcctgc cggattttcc catccttgcg
     9961 tgaaaccgaa agagacggcg gcgcggcgga caagctcgag atagcgtcct tcaagctccg
    10021 gacaggcggc ggacacgctt tctaccgtaa ccgtgaaacc gccgcccgac ccggcaaggc
    10081 gttccgcaat ttgcgtgtag atgagccaac gttgggcgcg caacattgcc gaatcgcaac
    10141 cggcagccgc tttatccaag gcaatcaggt cgcgccggtt ttggtggtga aggcaaaccg
    10201 ttactctgga gaggatatgt tgcccgtcca atatttgata cagtttgcgt atcagcagaa
    10261 tcaggcggtc aaactcctcc atctccgaca gggaatcagg atggtcggaa gcggtataaa
    10321 ccagtttgga caattttgcg gcaaacatat tgttcatcaa tcttccttgt cggttgaaac
    10381 cccgctcttc ggggcggtag aatcagattt gtttgggagg gacaactcct cccagatcag
    10441 gacgacacat aggctggtgc ttgatgtgtt gtccggcgag ttgaaacatt cagcaatcct
    10501 caaggggcgg cagttttgcc gaaacatatt ctacacggct tcaatgccgg acgataaaag
    10561 gaaattcata tgaaatggac cgacacccag cgcatcgccg aagaactcta tgacctgcac
    10621 ggcgaaacca tcgatcccag aaccgtgcgc tttacccaac tgcgcgacct gattatggca
    10681 ttgcccgaat ttgacgacga ccccgcccgt tgcggcgaac gcatcctcga agccgtgcag
    10741 caggcatgga tagacgaggc ggaataagtt tcgggaatgc cgtctgaaat gcggcggtac
    10801 gcggttcgtg cttctgtttg cagcgggaat ggttttacca gtctcctttt ttcagcctgt
    10861 ccagttggcg gcggtcgcgc ttggtcggtc tgccgtcggg ataggcggaa gtgatgcggc
    10921 tgaattggtc gagctgtttg cgctcttccc tcaatgttgc cgttttcgcg tcctcttcat
    10981 acagaagccg cgcctcggat gccgggcggc gttggtggtt caaaccttta accttgattt
    11041 tatagggaag ggaattgagc gtcaggtcga taatatcgcc gatgtctatg gttttactgt
    11101 ttttgacctt cgagccgttt acttgaaccc tacccagttc gatgtgcttt tgcgcaaggg
    11161 aacgggtctt gaaaaaacgt gccgcccaaa gccatttgtc cagccgcatg gcggaagaat
    11221 cgtgcttgtc tttcatacga ttttgtttga aataattgaa tttgtttcga gtttagcata
    11281 agatacgccg ccttataact agtatatatg cactaatcca ctgttttcca tgctgtccga
    11341 acacaaaaag agggtatgga aaagccgttt tggacaataa attaactgcg gaatatgcac
    11401 aaatagcgta tgatagcggc agaatctgtt gatgagagct tcattctatg aaacctgttt
    11461 ttttggattt tgaacaaccc atagccgaac tgaccaacaa aatcgatgag ctgcgtttcg
    11521 tccaagacga gtctgccgtc gatatttcgg acgaaataca ccgtttgcag aaaaaaagca
    11581 acgacctgac caaatcgatt ttcagcaaac tcacacccgc ccaaatttca caggtttccc
    11641 ggcatccgca gcgtccctat actttggatt acattgaggc actgtttacc gattttgaag
    11701 aactgcacgg cgaccgccac tttgccgacg attatgcgat tgtcggcgga ttggcgcgtt
    11761 tcaacggaca aagcgtgatg gtcgtcgggc atcagaaagg gcgcgacacc aaagaaaaaa
    11821 tccgccgcaa cttcggtatg ccccgtcctg aaggctaccg caaagccctg cgcctgatga
    11881 agacggcaga aaaattcggc ttgcccgtaa tgacctttat cgatacgccg ggcgcgtatc
    11941 ccggcatcgg cgcggaagaa cgcgggcagt cggaagccat cggcaaaaac ctgtacgaac
    12001 tgacgcgcct gcgcgttcct gttttgtgta ccgtcatcgg cgaaggcggt tcaggcggtg
    12061 cgttggcggt cgccgtaggc gattacgtca atatgctgca atactcgacc tattctgtta
    12121 tctcccccga aggctgcgcg tctattttgt ggaaaaccgc cgaaaaggcg gcggatgcgg
    12181 ctcaggcttt gggcattact gctgaccgcc tgcaaaagct ggacttggtc gataccgtca
    12241 tcaaagaacc attgggcggc gcgcatcggg atttcgggca aaccatgaaa aacgtaaaag
    12301 ccgttttgga aaaacaactg cacgaagcgc aaagcatccc gcttgccgat ttgctttcgc
    12361 gccgtttcga ccgcattatg gcttacggca aattttcgga acaataattc aggtagaaca
    12421 agcagcaagc agtttgtctg aaactgcttg ctttttcttt atcgggacgg aaccgtgctg
    12481 actttagatg cgtttgagca atgcttgaag gattgttttc ctcaaggtct gaatggaaaa
    12541 aaaacagcgg tggcattaag cggcggcttg gattccgtcg ttttgctgca tctgcttgtc
    12601 cgcgccggaa aaaagggcgg ttttattccg gatgcattgc atatccatca cggcttgagt
    12661 ccccgtgccg acgattgggc agatttctgc caaaactatt gcgatatgct cggggtgggg
    12721 ctggaaacgg ttaaggtctg cgtggaaaaa aacggtttgg gcatcgaggc ggcggcaagg
    12781 caaaagcgtt atgccgcgtt tgccgaaaaa ggctttgacg ttttggcgtt ggcgcaccac
    12841 agggacgatc aaatcgaaac ctttatgctg gcggtcgcgc gcggcggcgg tttgcgcgct
    12901 ttggcggcta tgcccgccgt ccgccctttt ggggaaaaag gcatcatctg gcggcccttg
    12961 ctgccttttt cacgccaaga tatatgggat tatgcccaaa aacacggttt gccgaatatc
    13021 gaggatgaaa gcaataccga tacggcttat ttgcgaaacc gcttccggca ccgtattttg
    13081 cccgaacttt cggcgcagat tccccatttc gggcggcatg tgctgaacaa tgtccgcgct
    13141 ttgcaggaag atttggcttt gttggacgag gtcgtcgttc aggactgccg ttgggtttgc
    13201 ggggccggtt atttcgatac ggcgcggtgg ctgacgtttt ccccgcgccg gaaaacccat
    13261 attttgcggc attttctgaa ggaaaacggc attcccgtgc cgaatcagaa tgcccttgcc
    13321 gacattgccc gggttttgac ggaggcaaaa accggacgtt ggaacttgca aggctttgaa
    13381 ttgcatcatt atgcaggcag gctgtttgtg ttccgactgg aaaaaacgga taaactgcgg
    13441 tttttgaaag acaggcagat aagcggaaat ttaagggaaa tattgacggg gcagggattt
    13501 gtgttgaagc ggcatccgtt tgggcttcct gagcatcttt tggagcagga cggaattttg
    13561 aggacggtag cggcatcgga tacgttggcc atgggcggca tccataagga tgtgaaaaaa
    13621 atccttcagg ggaaacgggt tttgcctgtc ctgcgcccaa tttggccgct tgttgccgac
    13681 agcggaaacc gtccattggc gttggcaaac tgttgtgcgg atttccaata ctcggtttca
    13741 gacggcattt tgcccgtcca tcctgacttt cccattttat tttgataata tcgcaaacag
    13801 atttcggcgg cgttcagtcg ggtattgtcc ggttgcatat ttctaaaagg cttgtgaagt
    13861 gaaacacatc agttcgacca ataatgaaca catcagacac ctgcaccgcc tgttgtcgca
    13921 aggaaagttc agacggcaat acgcccaaac cgttttggag ggcgtgcacc tgcttcaggt
    13981 tttcctgcaa tccggcggga tgccggtcgg ggtatatatt cccgaagcga aaatgccgtc
    14041 tgaagaagtc cgtaaattga cggcggtttt gccggaagac gggttttttt ccgtttcaga
    14101 cggcatattg aagaaaatca gcagcctgac ttgtgcggat gatgtgcttg cgctgattga
    14161 tattccagat gcgggtgctt tgccggccgg cggcgattgc gtggttttgg acggcgtgca
    14221 agacccgggc aatgtcggca cggtgttgcg aagcgcggcg gcggcgggaa tcggcgcggt
    14281 cattttgggc aaaggttgtg cggacgcgtg gtcgcccaaa gtgctgcgag ccggaatggg
    14341 cgcgcatttc ttgtcggaga tttatccgca ggcggatttg gaaatatggt tggtgcgcta
    14401 taaaggccgt gtgtttgcca ccgccttgcg cgaggaaaag caggcggttt tgtacggcga
    14461 agatttgtgc gaaccgacag cctgggtgtt tggcaacgaa ggcgcggggg tcggtaaagc
    14521 agttttagat agggcggaca agtgtgtcag gataccgatg cacgatgcaa ccgagtcttt
    14581 aaatgtcgcg atggcggcga caatctgcct gtttgaacaa atgcgccaac gggcggcgta
    14641 ttgaggaaga gaaatgccgt ctgaaaaaat ctattacggc gtattgattt tcttatgtat
    14701 cgcttctatg ctgctgtcgc cgttttttta tgcgggtgct ttgaagccca agaaggcggc
    14761 attgcggaag gacgggcagt ggaaactcat ctgattgtcc aatgccgtgg cggcggcggt
    14821 tttggcttgg gtgtggtgga aatggttttg acagatattg ctcaaaatcg tgctaatgga
    14881 atccgaacaa ataaagaatt tggtaaaaaa tttgttaaat caacgaatta aagttttgtg
    14941 gaaaacaaaa cagctctaag caaatagggc gtttgtcggt aaatacggaa gagttgcggc
    15001 attatcgggc atctttaaca agtagtgccg tcttgacagg caatcggttt ttatgggcag
    15061 cttgcaaaat cgcggatata aaattgcgaa tcggttaaag tgtggggacg ctatgaaaaa
    15121 ttgcgaattt tttatgaccc gacaagggca atctatgata gcggtgcaga ttacttaact
    15181 agggaaaaac atagattagt cgtaattgca aatagtgctt gggggctatt gcttaattta
    15241 tcttgttatt atgacgaggt tttggaaaag cggaaaatac cgttcggcaa acaggaaatt
    15301 gatgacgata tggacaaagt gtccgccctt aagcggaagt ttaaagatat ttctgaaatc
    15361 aaagtagggg atggttggga atacccgttc aattatgagc agggaatgaa agaattagat
    15421 gaagtgctat tgaaatacat tccctttttt gaagaagaac gataaaggag gttgatatgc
    15481 gcgtatctaa aataattgga agtatgttgc ttgttacagc ggttcagacc gtattttcgg
    15541 caaatgttta cgcgtgccgc cataatggta aaaccagtta cagccaaact ccgggaaaac
    15601 attgtaccaa cgcgggtttg gggcgggacc gggtgtacag ttcggttaga ccggcagtaa
    15661 aagacagggc ggaagacgca ggggtcggcg attattcgga cacggtgagg gacgaacacg
    15721 tccaaaatcc gaaaggaaat gcacagaaag acggttcggc tgccggcatc aagccgcact
    15781 gattgaagcc gaatcagccc ttgcgctgtc ggacggcaaa atttgaacga ttggggaact
    15841 ttgatgaaac acatccatat tatcggtatc ggcggcacgt ttatgggcgg gcttgccgcc
    15901 attgccaaag aagcggggtt tgaagtcagc ggttgcgacg cgaagatgta tccgccgatg
    15961 agcacccagc tcgaagcctt gggtatagac gtgtatgaag gcttcgatgc cgctcagttg
    16021 gacgaattta aagccgacgt ttacgttatc ggcaatgtcg ccaagcgcgg gatggatgtg
    16081 gttgaagcga ttttgaacct cggcctgcct tatatttccg gcccgcaatg gctgtcggaa
    16141 aacgtgctgc accatcattg ggtactcggt gtggcgggga cgcacggcaa aacgaccacc
    16201 gcctccatgc tcgcatgggt cttggaatat gccggcctcg cgccgggctt ccttattggc
    16261 ggcgtaccgg aaaatttcgg cgtttccgcc cgcctgccgc aaacgccgcg ccaagacccg
    16321 aacagccaat cgccgttttt cgtcatcgaa gccgacgaat acgacaccgc ctttttcgac
    16381 aaacgttcta aattcgtgca ttaccgtccg cgtaccgccg tgttgaacaa tctggaattc
    16441 gaccacgccg acatctttgc cgacttgggc gcgatacaga cccagttcca ctacctcgtg
    16501 cgtaccgtgc cgtctgaagg cttaatcgtc tgcaacggac ggcagcaaag cctgcaagat
    16561 actttggaca aaggctgctg gacgccggtg gaaaaattcg gcacggaaca cggctggcag
    16621 gccggcgaag ccaatgccga cggctcgttc gacgtgttgc tcgacggcaa aaccgccgga
    16681 cgcgtcaaat gggatttgat gggcaggcac aaccgcatga acgcgctcgc cgtcattgcc
    16741 gccgcgcgtc atgtcggtgt cgatattcag accgcctgcg aagccttggg cgcgtttaaa
    16801 aacgtcaaac gccggatgga aatcaaaggc acggcaaacg gcatcaccgt ttacgacgac
    16861 ttcgcccacc acccgaccgc catcgaaacc acgattcaag gtttgcgcca acgcgtcggc
    16921 ggcgcgcgca tcctcgccgt cctcgaaccg cgttccaaca cgatgaagct gggcacgatg
    16981 aagtccgccc tgcctgtaag cctcaaagaa gccgaccaag tgttctgcta cgccggcggc
    17041 gtggactggg acgtcgccga agccctcgcg cctttgggcg gcaggctgaa cgtcggcaaa
    17101 gacttcgatg ccttcgttgc cgaaatcgtg aaaaacgccg aagtaggcga ccatattttg
    17161 gtgatgagca acggcggttt cggcggaata cacggaaagc tgctggaagc tttgagatag
    17221 cccgggcgat gccgtctgaa agcccttcag acggcatcgc ccggctgcgc ggcacaaagg
    17281 cggaaaaacc gtttgccccg tattttcaaa cgcgttacac ttgccgccgc tgttttcagc
    17341 catttgatta cccgcaaccg ccgtcattgc gccggcggtt tgcctgtcag cgtcattgcg
    17401 ccgctgtaaa tacgaaagaa cacattatga ccgtatcccc cgtcgccttg cgccgtaaga
    17461 ccgagtgcaa gcctcatccc accgcgcgct attggaaaaa atgcgatgtc gaagccctgt
    17521 tcggacttcc cttcctcgac ctcatttacc aagccgccga aatccaccgc caaaatttca
    17581 acccgcgcga aatccagctt tccacgctgt tgtccatcaa aaccggcggt tgtcccgaag
    17641 actgcgccta ttgtccgcaa tcggcgcacc acaataccaa tctgggcaaa gagcagatga
    17701 tggatgtgga tgaaatcgtc gaaaaagcca aaatcgccaa atcgcgcggc gcaagccggt
    17761 tttgtatggg cgcggcgtgg cgcggcccta aacccaaaga cgtggagacg gtttccgcaa
    17821 tcatcaaagc cgtcaagggc ttgggtatgg aaacctgcgg cacgttcggt atgctcgaag
    17881 aaggtatggc ggaagacttg aaagaggcgg gcttggatta ttacaaccac aacctcgaca
    17941 ccgaccccga ccgctacaac gacatcatcc acacccgcca acacgaagac cgaatggaca
    18001 ccttgggcaa agtccgcaac gccggtttga aagtctgctg cggcggcatc gtcgggatga
    18061 acgaaacccg cgccgaacgt gccgggctga ttgccagtct cgccaatctc gacccgcagc
    18121 ccgaaagcgt gccgattaac cggttggtca aagtggaagg cacgccgctt gccgatgccg
    18181 aagatttgga ctggacggaa tttgtccgca ccatcgccgt ggcgcggatt acgatgccgc
    18241 aaagttatgt ccggctgtcg gcagggcgca gcaatatgcc tgaagcaatg caggcgatgt
    18301 gctttatggc gggcgcgaac tcgatttttt acggcgacaa gctgctgacc acgggcaatc
    18361 ctgatgagga cggcgacaga atcctgatgg aaaagctcaa cctgtatccc ttgcagtttg
    18421 aaccggaagg cgaggtcgcc gaagtggaaa aagcctctgg gattaaagtg gattattgac
    18481 gattgaaaaa tgccgtctga aacccggaaa aaggctttca gacggcattt gtccggacgg
    18541 catttccaat atctttttac cggcgcgtga tgctgccgtc gggcgagaca tccagcccgt
    18601 tccccttggg gaaatcgctg cggttcagat aaataatccg cccgataacg gttttctcgg
    18661 ggtcgatttt ggggatttta tcgccgactt gagtgatggg gataatgttg ccggaaacga
    18721 accgcccctg tttgtcggtg ataattttaa aaatgggggc gatgccgctg atgccggagg
    18781 tgttgattgc gccgtaggtg gcaaagttgc cgccgctgta ggagatgaag cggtcgcggt
    18841 aaagttcgac ggcgcgagta acgtgcggcc cctgcccgaa tacgacatcc gcgccggaat
    18901 cgacggcaag ccgcgcaaac tcaacgacgt tgcccctgtt ttccccatag aagatttcgg
    18961 tatcgaacgg caggtgttcc gcctgtttcc cttccgcgcc gccgtggaac atcacaatga
    19021 cgatgtcggc tttctgtttg gttttggtaa tccgttttct aactttggca taatcgttca
    19081 gtttgacggc ggcaaggttg ggggcgaagg agacgaagcc ggatcttacg ccgtttttct
    19141 tcaggatggc ggtttcaaac ctgttttcga tgcccgaata tttgatgttc aattcgtcaa
    19201 ggttcgccct ttatgccgtt tccgcaccgc aaaccgccgg ggtcaagccc tcggcgcatc
    19261 ctgccggaac ggaatccccg tgctgccgat tgacggttca aaccccgcgc ccgtttcaaa
    19321 taccggcgat gtggacggac aggatgcgcc tgacgaaaag acagccgata ccgtttccat
    19381 tatcggcgtg ggcgacatta tgctcggcag caattatccg gtcgattacc tgcccgatac
    19441 caatattctg aaaaacgtcg aatctgcctt gcaagacgcg gacattaccg tcggcaacct
    19501 cgaaggcacg ctgtttgacg aaggcggtac gccgaaaaaa tgtgcaaacc cccaaaatat
    19561 gctatgcatt ccgaacgccc tccgcatacg ggcaatacct tgccgacgcg ggattcgact
    19621 acctcagctt cgccaacaac cacagcaacg acttcggcgc gcaaggcatc acggcaacgg
    19681 cggcgagcgg cagctctttt acatactcga tcgcgctaaa gccgctgccg ataacgaggc
    19741 caaaattgcg gaaaataccg ccatcgccca gataaatttg tccatcatca gacctttact
    19801 gttcagacga gacagcattt gccgcacgtt ttggggctta tctttcgatt tgcgctacgt
    19861 cgcgcaccgc gcctttgtcg gcggaagtcg ccatcgcgcc gtaagctctt aatgcggcgg
    19921 agacgtagcg gtcgcggttt ttaggcttcc atgctttgct gccgcgcgct tccatttcgg
    19981 cacggcgtgc ggcaagctct tcatcggaaa tggcaaggtg gatgctgcgg ttggggatgt
    20041 cgatttcgac ggtatcgcct tcgtgtacca aaccgatcgc gccaccttcc gccgcttcgg
    20101 gcgaggcgtg tccgatggac aaacctgatg tgccgccgga gaagcgtccg tcggttaaga
    20161 gagcgcaggc tttgccgagg cctttagatt tcaggtagga agtcggatac agcatttcct
    20221 gcatgcccgg gccgcctttc gggccttcgt agcggatgat gacgatgtcg ccagcgacga
    20281 tttggttgcc caaaatgcct tctactgcgt cttcttggct ttcaaacacg cgggcgcggc
    20341 cggtgaattt gaggatgctc tcgtccacgc ctgcggtttt taccacgcag ccgcgctcgg
    20401 cgatgttgcc gaacaagacc gccaaaccgc cgtcttgcga gtaggcgtgt gccacgtcgc
    20461 ggatacagcc tttttcgcgg tcgaggtcga gggttttcca catacggttt tgcgagaacg
    20521 cttgggtggt gcgtacgccg cccggcgcgg ctttgaagcg ttcgatggca cgggtgtttt
    20581 cgggattggt cacgtcccat tgttcaatcg cgtctttcag cgtcggcgcg tggatggtgt
    20641 gcacgtcggt gtgcagtttg cccgctttgt ccagttcttt caggatggcg aagataccgc
    20701 cggcgcgatg cacgtcttcc atatagtagt cgtggttgtt gggtgcggtt ttgcagatgc
    20761 agggcacgac gcggcttaag cggtcgatgt ctgccatttt gaaatcgaca ccggcttcgt
    20821 tggcaacggc caacaggtgc aaaatggtat tggtgctgcc gcccatcgca atatccatcg
    20881 tcatagcgtt ttcaaacgct tttttggtgg caatgctgcg cggtaacacg gtttcatcgt
    20941 tttgctcgta atagcgtttg gtgatttcga caatcatacg gccggcttcg aggaacaatt
    21001 ctttgcggcc ggcgtgggtc gccaaatacg aaccgttgcc gggcagggaa aggccgagtg
    21061 cttcggtcag gcagttcatc gagtttgccg taaacatacc cgaacacgag ccgcaggtcg
    21121 ggcaggcgtt ttgttcgact tcctcgactt gccggttgct gacattgtcg tccgccgatt
    21181 caatcatcgc gtcaatcaag tccaaacggc gttcgggctg gatgtttgcc acgccgataa
    21241 ccttgcccgc ttccatcggg ccgccggaga cgaagatggt ggggatgttc aggcgcatcg
    21301 cggcaatcag catgcccggg gtgattttgt cgcagttgga aatgcacacc agcgcgtcgg
    21361 cgcagtgggc gttgaccata tattcgatag agtcggcaat caaatcgcgg ctgggcaggg
    21421 agtacagcat gccgctgtgt cccatagcga tgccgtcgtc gatggcgatg gtgttgaatt
    21481 ctttggcgat tgcgccggct ttttcgattt cgcgggcaac cagctggccc atattgtgca
    21541 ggtggacatg gccgggcacg aattgggtga aggagttggc aacggcgatg atgggcttgc
    21601 cgaagtcggt ttccatcacg ccggtggcgc gccacaatgc acgtgcgccc gccatattgc
    21661 ggccgtgggt ggaggttttg gagcggtatt caggcatagt gtgtttcctt gtgcctatac
    21721 cgtctgaaag acagggctgt ttcagacggt atcgggtacg gttttttaga gtgggaaaag
    21781 agggtatttt ataccaagta tcggaatttt gcgggattga aacggcgtgc ggcaaaaaag
    21841 aaaatccccg caggaatgcg gggacgggtt caggcgcggg caatcgcgac ggctttggat
    21901 gcgtcccaaa aatcaacggg tgcatttaat acgggtttga cgatgcccgt ccgtatggcg
    21961 aaatcgccga aaccttcgcc gatattgcgt tctgccgccc atttgccgat caggtcgtcc
    22021 aattcggcaa ggatttcggg cagggtgatg ttttctttgt aaagacgggg gatgcgtacg
    22081 ccttcacggt cgccgccgat atggaggttg tagcgtccga cggctttgcc gaccagtccg
    22141 atttccgcca acatcgcccg tccgcagccg ttcgggcagc cggtaatgcg ggtaacgatg
    22201 tagtcgtccg acgtgccgtg tttcgccata atcttatcca gctcgccgat gaagtccggc
    22261 agcacgcgtt cggcttccgc cattgccagc gggcaggtcg gaaaggaaac gcaggacatc
    22321 gcattttcac gcagcttgct gacatcgttg cggattaatc cgtatgttcg ggcaaattct
    22381 tcgatttttg ctttgtctgc ttcggcgaca tttgccacga tgaggttttg gttggcggtg
    22441 atgcggaaat cgcctttgtg gattttggcg atttccaaca cgccggtcag aagctgtttc
    22501 ccgccttcgt caaccaaacg cccgctttcg atgaaaaggg ttaaatgcca gttgccgtct
    22561 atgcctttca cccagccgat gcggtcgccg cgcccggtaa atttgaacgg gcgtacgggt
    22621 tcgaacggca tacccatacg gcgttcaact tccgcgcgga agttgtccaa gcccatattt
    22681 tgaatggtgt agcgggtgcg ggcgtttttg cggtcgctgc ggttgccgaa gtcgcgctgc
    22741 gtggttacca ccgcttcggc ggccttcagc gcgtgttccg gaggcacgaa acccagttcc
    22801 agtgaaatgt tcggataggt tttggtgttg ccgtgttcca tcgaaagccc gccgcctgcc
    22861 aaaacattga agccggcaag ctgtccgtta ccgtctgaaa cggcgacgaa atccaaatcg
    22921 ttgccgtagc agtccacatc gttcaagggc gggatgacga ctgcggtttt gaattttcgc
    22981 ggcagatagg ttttgcccaa aatcggctcg tcttcttgaa ggaagtcgtc ggaactttga
    23041 acttttttgc cgtccaccca cacatccaga taaccgcgcg tgcgcggcag caggtgttcg
    23101 gaaatctttt tcgcgtattc gtaagcctgc cggtgcagtt cggactcgat cgggttggac
    23161 gtgcaaagca cgttgcggtt catatccgcc gccgtggcga tggaatccaa acccagtttg
    23221 tgcaagaggc ggtgcatcgt ctgcaacttg gctttcggca cgccgtgaaa ttggaaggtt
    23281 tgccggttgg tcagccggat ggagcggtaa tgactgtttt cccgggcaaa tttgtccagt
    23341 tctatccatt gggacggttt gatgatcccg cccggcagcc ggcagcgcaa aagcataaat
    23401 ttcaagggct cgagttttgc ctcggcgcgt tcggcgcgga tgtcgcggtc gtcctgctca
    23461 tacataccgt ggaagcggat gagttggaag ttgtcgcctt tgaagccgcc cgtgagcggg
    23521 tctttcaaat cgtccaaaat cgtgccgcgt aaaaaattgc tttcggtttt cagacgttcg
    23581 ttgtcggata gcggtttttc ttgccacgcc aaaccttttg tcttggtctg tacggtcatt
    23641 ttgtgttcct cccgattata tttaatcaat aaacatcacg ctgatagcgt ttttcttcgc
    23701 gcagcatatc caaatattct tctgcgccct cttcgtccaa atgtcctgcc ccgataatca
    23761 catccagcaa ggcggcttcc acgtcttttg ccatttttgc cgcatcgccg cacacataga
    23821 tatgcgcgcc ttcctgcagc cattgccaaa gtccttccgc ctgttcgcgg attttgtcct
    23881 gcacatagat tttttcttcc tgatcgcggg accaggcgaa atcgtacctg tgcaggaagc
    23941 cgtctttggc aaactgctgc cattcggttt gatagagaaa atcacgggca aaatgcggat
    24001 tgccgaaaat cagccagttt ttgccttccg cattttctgc ggcacgttgt tggacgaaag
    24061 cgcggaacgg tgcgacgccg gtgcccgagc cgatcatcac aatcggcttg cggctgtctt
    24121 cgggcagcct gaagccgtcg ttgcgttcca caaacacgcg caccgtgccg tcctcttcca
    24181 gccggtcggc aaggaaaccc gatgcgccgc ccgttctggc gcggccttcg tgttcaaaac
    24241 gaaccacgcc gacagttaaa tgcacttcat cgcccacttc cgcctgtgct gaagaaatcg
    24301 aatacaaacg gggtgcaagc ggacgcagta aacggatgaa ttgttctgcc gtcaggcttg
    24361 ccgggaagcg gtgcagcaca tcgacaatag gcgtgttttg cacgaaatcc tgcaaaacgg
    24421 cgttatcggc aatgatttta tcgagttctt cataatgggc gaacgcggca tagcctttga
    24481 cgaaagccgg agtgttttgc gtgagttcga aatgagatga aagtgcgcgc gcaaccggca
    24541 tcatctttcc gcccgcctgt atttccgttg ccggatcgat gccgagcagg tctaggattt
    24601 ccctgaccag tgccggatcg ttgtcaaacc aaacgccgag cgcgtcgccc gggaggtagt
    24661 gcaaatccga accgctcaaa tcgatttcga tgtggcgcac gtctttatcg gattggcggg
    24721 cggtgatttt ctgattggcc agcagggcgg cgggaaaggg ggctgccttg cagtacctgc
    24781 catccggtgc cgtctgaagg ccggcggggg gcgttgtctg cggcgcgggc gttgcccggt
    24841 tttttgcggc ttcttctttt aagagtgcgg cgatattatc tgtccaggcg tttgcggagg
    24901 cggtaaagtc caaatccgca tcaacgcgtt cgagcagccg ttttgcgccc aattcttcaa
    24961 aacgccggtc gaaatcttta cctgcctgac agaaattcgg ataggaactg tcgcccaaac
    25021 ccagtacggc aaattggagt ttgtccaatt tcggggcttt tttgccgttc agcagtttgt
    25081 gcagcacgac ggcttcttcc ggcggttcgc cttcgccttg ggtggaggta accagcagca
    25141 ggcggcgttc gccggcgatg tttttcgcct tatagtcttt cagttcggcg cgactgactt
    25201 ggatgccggc ggcttccagg ctgtccgccg ctttgtcggc aacggatttc gcattgccgg
    25261 tttgcgaggc ggaaaggacg gttacggaaa aaggttctgc cgccggcaat gccgtctgaa
    25321 gcgcgggcag tcctgcagat gccccgtttc ctgcttttgc ccaagcgtag ccggacagcc
    25381 acgcccattg tgccgcgtcc agccccgaca ggagctgcgt gatttcgggc ggcagaggcg
    25441 gtaatggcgg atttgtgttc tgcatatcgt gttcactcat aaaatcatac ctgccgcaac
    25501 agtgccgtat gtcgcttcgt ctatcaggat aaacgaaccg gcggcggtgt tttccgcata
    25561 aggcgttgcc gtaacgggtt tttgaaggtt gatgcggact ttggcgatgt cgttcatctt
    25621 caaggattcc gcgccggcct cttgttccag cgtgcggaca tccaaaacgc tttcaatttc
    25681 cccgactttt gccggcacgg tttgcgtgcc gtgcttgagc aggtatttgc gcgcggtgtt
    25741 gagcggacgt tcgtcaaacc agcaaagcgt ggcttccaga tgtttttgcg gggcgagcgg
    25801 ggaattttta tcgacaaaaa ggtcgccgcg cgaaacatcg atgtcgcggt ccagccggag
    25861 ggttgccgcc tcgccggcaa aagcctgcgc cacttcccct ttcggcgtga tgatttcgga
    25921 cacttcggcg gtcagcccgt tcggttcgat gcggacggtt tgcccgacgg cgaccgaacc
    25981 gcgttcgatg cgcccctgat agcctcggaa atcatcggcc ttgtcggcat cttggcggac
    26041 gaccagttgc acggggaaat aaaaatcgtc ggcggtgcgg ctgacttcgt ccgcccccgg
    26101 cagggtttcc aaaatggaca ataagggttc gcctttatac caaggcatat tgccgccggg
    26161 gtaaacaatg ttgtcgccca agagtgcgga catcggtacg aaatgcgcgt ctttcaaacc
    26221 gagctgttcg gcaagtcggc ggtatgcctc cacaatggcg ttgaatttgt cttcgctgta
    26281 atccagcagg tccattttgt tgaccgccac cacaatatgc gggcagttga gttggcggag
    26341 gatggcggaa tggcgtttgg tctgcggcag aagctgcaag ggctgcgcgc cgaaatccag
    26401 ttgggatgcg tcaaccagca cgactgccgc cgaagcggtg cttgcgcccg taaccatatt
    26461 gcgcgtgtat tgttcgtgcc ccggcgtgtc ggcgatgatg aatttccgtt tcgccgtgga
    26521 aaaatagcgg tatgccacat cgatcgtaat gccctgttcg cgttcggctt ccagtccgtc
    26581 ggtcaggatg gagaagtcta tggcttcttt caaacctttg cttttgccgg attccaaggt
    26641 tttgatttgg tcggacagca gggctttgct gtcgtagagc agtcgtccga tcagggtgct
    26701 tttgccgtca tcgacgctgc cggcggtaat gaagcggagc ggggtttggt gttgtgccgt
    26761 catattttct tcctcatatc tgcttaaagg gtttttgaaa tttagaaata gccttctttt
    26821 ttgcgttttt ccattgccgc ctcgcttgcc tgatcgtcca gccgggtcgc gctgcgttcg
    26881 gaaatgtcgg caaccgctgt ttctctgata atctccgtcg gcgtggacgc ggtgctttct
    26941 accgggcagg tgcagctgat gtcgccgacg gtgcggaagc ggacatcaag gatttcggag
    27001 gtttcagacg gcattttcgg ggtgagcggc gttacaggga ccagcagccc cctgcgtctg
    27061 accacttcgc gcctgtggct gtaataaatc ggcggcagct cgaggttttc gcgggcgatg
    27121 tattgccaga tgtcgagttc cgtccagttg gaaatcggga agacgcgcat attttcgcct
    27181 ttgtgcagcc tggtgttgta cagcgaccac agctcggggc gttgcgcctt cggatcccat
    27241 tgtccgaact cgtcgcggaa cgagaaaatc cgttctttgg cgcgggcttt ttcttcgtcg
    27301 cgccgcgcgc cgcccataag cgcgtcgaag ccgtttgcct cgatggtttc caacaaggta
    27361 accgcctgtg ccgcattgcg cgaatcggtt tctttgcgta agaccaccgt gcctttggca
    27421 atggagtctt ccacgcgccc cactatcagg cgggcattga gttttgccgc ctgcgcgtcg
    27481 cggaaggcaa tcacttcggg gtagttgtgt cccgtgtcga tatgcaccag cgggaagggc
    27541 agtttcaccg gccggctgcc cagccggaag gctttgcagg cgagggcgag caggaccacg
    27601 gaatctttgc cgccggaaaa gagcagggcg gggttttcgc attctgccgc cacttcgcgg
    27661 atgatgtgga tggattcgga ttccaaccag tcgagttggg cgttgttcgg ttcggttttc
    27721 gtcataccat attccttatt tcttctgtct gatatttatg aattatttgt gcagcccgca
    27781 ttctttgctg tttctgcctt cccaccacca ccgcccggcg cggatgtctt cgcccgcctt
    27841 gacggggcgg gtgcaggggt cgcagcctat gctgggaaat ccttgccggt acaaatcgtt
    27901 gtaaggcaca ttgttggcga ggatgtatgc ccacacgtcg tgttccgacc agtcgaaaat
    27961 cgggttgtat ttgccgatgc cccgtccggc atcgtattcg gcaaacggca gttccgtgcg
    28021 tgtggcggat tgttcgcggc gttgcccggt aagccaggcg tccgcgcctg caatggcgcg
    28081 gttgagcggt tcggtttttc ggatgcggca gcattcgcgg cgcgcttcaa cgctgtcgta
    28141 aaaggcaaac ctgcctttgc tttccacata acggtcggca tcttctcgaa ccggccggaa
    28201 acgctttatc cgcaaatggg gatatgcgcg tccgagcctg tccagcaggt tcagggtttc
    28261 cgtgtggagc agccccgtat ccaaggtaaa aatgccgata ttgaggtttt cgccggcgat
    28321 aaggtcggta atcaccatat cttctgccgc aaggctgctg gcaaaccgtg catcccggtg
    28381 gctgccgaca atccggtgca ggcgttgttt gagggtttcc gttttttccg caagggcggt
    28441 ttcgccgccg gatccgatat gcggtatctg ccacagggcg ggtttgaaca gtgtcgtttc
    28501 catttttccc gccttatgcc gcccgttgtc cggcattcag tccgcccaat gcgggatacg
    28561 tctgcccgac ccggttttct ccttcgccgt tttcaccgaa ccaggcgagt ttttcgtgca
    28621 gccccaccac ttcgcctatg acaatcaatg ccggattcgg cgcggtttcg gcgagttcgg
    28681 caaggttggc gagcgtgccg gttgcggttt tttgagccgg cagcgtgcct tggctgataa
    28741 cggctgccgg cgtgtcgggc gagcgtccgt gctgttgcag ccgttcggca atcagggcgg
    28801 ctttgagcgc acccatataa atcaccaagg tctggcggct gcgggcgagg gtctgccatt
    28861 cgatgtcggg cgcatccgcc ttgcggtggc cggttacgaa aaccgcactt tgggcataat
    28921 cgcggtgcgt gagcgggatg ccggcatagg cggtcgcgcc gacggcggcg gtaatgccgg
    28981 ggacgaccga aaacggaatc tgatggcgtg ccaaggtttc caattcttcg ccgccgcgtc
    29041 cgaacacgaa agggtctccg cctttcaaac gcaccacgcg cctgccttcg cgggccagcc
    29101 tgaccataag cgcattggtg tcctcttgcg gggtgcgctc gccccgggcg cgcttgccga
    29161 caaaaatccg ttccgcatcg cggcggacga gggacagtat gccgtctgaa accagcgcgt
    29221 cgtaaagcac cacgtctgcc tgctggattt cctgcagccc tttgagcgtc agcagccccg
    29281 catcgccggg acccgcgccg accagcgaga cggagccgcc ttgatcattt tgacgacttt
    29341 gttccaattg gcctgccaat tcccgttcgg caagggtgtt ttgccggttt ttgacgaggg
    29401 cggcgaaacg tccgttaaac tgcttttccc aaaagcggcg gcgttcggta acggatttca
    29461 gtttgccctt gacggcatcg cgccaccttc ctgaaatttc cgccatatcg cccaaagacg
    29521 gcggcagcag ggcttccagc ctttcacgca gcagtcgggc gaggacgggc gcgctgccgg
    29581 agctggaaac ggcaatctga accgggttgc ggtcgataac cgacgggaag atgaagctgc
    29641 aatggtcgcg gtcgtccacc acgttgaccg gcttttggca gctttcggca agatggaaaa
    29701 cgcgccggtt gagggcttgg tcgctgcttg ccgcaatgat gaggaaaacc gtgcggatgt
    29761 gttcggcacg aaattcttcg gcaagccaca ggattttgtt ttccgccgcc aacgcggaga
    29821 gttcggcatt caggtgtttt gcggcaaccc tgacctctgc gcccgccttc agcagcaggc
    29881 tgattttgcg tgcggcgacc gcgccgccgc ctacgaccaa tacggggcgg ccggcgaggt
    29941 tggcgaaaat agggaaataa ttcactggct gactcctttg ctgtttgccc gcaccttgtt
    30001 tccgatacgg tgcgtcgcgg catttttgtc ggaatgcggg tcattttaga caaaaggatt
    30061 ttccccggtt aaataataaa aaggtatttg ttagaagctg aaagctatat gggggcggct
    30121 gcggatgcgg cggttttccg ttttataacg gtttcggaag aaaaacggcc tgaagccgtt
    30181 tcgggcattc agaccgtttg cgtggtgagg ggatgccgtc cgaagggcga aaagggcttc
    30241 agacggcatt gatgtcgggt ttcaggacag gagcaggatg gcggctgcgg caagcgaggc
    30301 aaccgataat gcggcggcaa gcgcggcttt gcctgcaaag cggattgagg ttttgccttc
    30361 gatgtatttg aagccggtta tcatcgggag gatgaggttt ttctttttga atacgcggta
    30421 tgcggcgacg gcggcgatgt ggattgcaga aaaaacggcg agcagcttga aaaagttgag
    30481 gtggattttc cgcataaggc tgcccgtatg ttcggaaacc aaatggttga ggtagccgtt
    30541 ggtgctgaag gtgttttcat cggcggcaaa aagcccggtg ccgacttgga aggacacggc
    30601 ggccaaaagc gcaacgacca tcagtgcgcc caaggggttg tgtccgggct ggatgtgttc
    30661 gggaataccg tttttcagat agccgcgtat gcctgcccag ccttggacga aacgggaaaa
    30721 acgggcggta tcgctgcccc aaatgcccca gcagaggcga aatacgagca ggaaaaggac
    30781 gaacagcccg acgcgcgtgt gccattgcag catatcgccg ccggctttcg cgctatacca
    30841 cataaagggc agggacgcgg caagcagcca gtggaaaagg cgggtgggga ggtcccagac
    30901 tttggttttg tttttcataa tcggtttccg gcggtagaat cggtttgttt tcgagcctta
    30961 ttttaaccga ttggaggggc aatgtttccc gtttttcatc tttcaggcga gagccgccgc
    31021 cagatgcttc agacggcatt gcgttttccc catgttttca aagcccgtgc ggaagattcg
    31081 cacaaaggga ctttcggcac gctcgccgta gtcggcggat cggcagggat gagcggcgcg
    31141 cccgtattgg cggcatcggc ggcaatgtat ctcggctgcg gcaaagtgtg ggcgggtttc
    31201 aatcaggata cgctaccttt tgccgttatt gccggttttc ccgagattat gctggatacg
    31261 gcggacagtt tggccaaacg tcaagatata aacgcctggg ttgtcggttg tggattgggt
    31321 acaggtaggg cggcggtcgg aacgcttgcc ggaattttga cggaacacac ggacaagccc
    31381 gtcgttttgg atgcggatgc gctgaacata ttatcaaccg atgccgaaac ccgaaatctg
    31441 gcgcgcgggt gtaaaaacct gattttaacg ccacaccccg ccgaagccgc gcgcctgctt
    31501 ggaacgacgg ttgcgcaggt tcaggcggat cggacggcgg cagtgaggaa gataggggca
    31561 attttcggcg caaccgtggt tttaaagggg cacaaaacat tggttgcctc acccgatacg
    31621 gaaatctatg tcaacgaaag cggcaacgcg ggattggcaa cggcgggcag tggcgacgta
    31681 ttgggcggca tcatcggcag tctgctcgca cagggcgtgc cggtttttga agccgcctgc
    31741 gcgggcgcgt ggctgcacgg cgcggcggcg gatgtcataa aagaatcggc aggcattgcg
    31801 gcagggctgt tggcagggga aatcgctccg gcggcaaggt ggctgcgcaa ccggataact
    31861 aaaagtatgt aagaagatat agtggattaa caaaaaccag tac
//
LOCUS       DAWGRZ010000004        30492 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000004 DAWGRZ010000000
VERSION     DAWGRZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30492
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.004"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(105..2855)
                     /gene="gyrA"
                     /locus_tag="WHT44_000123"
     CDS             complement(105..2855)
                     /gene="gyrA"
                     /locus_tag="WHT44_000123"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ2532367.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            complement(2953..3453)
                     /gene="hscB"
                     /locus_tag="WHT44_000124"
     CDS             complement(2953..3453)
                     /gene="hscB"
                     /locus_tag="WHT44_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ2532368.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            3535..3756
                     /locus_tag="WHT44_000125"
     CDS             3535..3756
                     /locus_tag="WHT44_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ2532369.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKRGSFRRSRRSL"
     gene            complement(3716..4036)
                     /gene="iscA"
                     /locus_tag="WHT44_000126"
     CDS             complement(3716..4036)
                     /gene="iscA"
                     /locus_tag="WHT44_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ2532370.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(4123..4509)
                     /gene="iscU"
                     /locus_tag="WHT44_000127"
     CDS             complement(4123..4509)
                     /gene="iscU"
                     /locus_tag="WHT44_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ2532371.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKGDDSVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            complement(4773..5987)
                     /locus_tag="WHT44_000128"
     CDS             complement(4773..5987)
                     /locus_tag="WHT44_000128"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ2532372.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHSFG
                     WEAEEAVEKARADIAALINADSKEIVFTSGATESNNLAIKGAAHFYKSKGNHLITVKT
                     EHKAVLDTMRELERQGYEVTYLDVQENGLVDLDVLKAAIREDTILVSVMWVNNEIGVV
                     QDIPAIGEICRERKIIFHVDAAQACGKVPVDVEAAKVDLLSMSGHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELAQDTAHYLKLRDI
                     FLKGIEGIEEVYINGDLEHRVPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            complement(6016..6462)
                     /gene="iscR"
                     /locus_tag="WHT44_000129"
     CDS             complement(6016..6462)
                     /gene="iscR"
                     /locus_tag="WHT44_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ2532373.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            6753..7925
                     /locus_tag="WHT44_000130"
     CDS             6753..7925
                     /locus_tag="WHT44_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ2532374.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTQRRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            complement(8074..10851)
                     /locus_tag="WHT44_000131"
                     /pseudo
     CDS             complement(8074..10851)
                     /locus_tag="WHT44_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(10841..12946)
                     /locus_tag="WHT44_000132"
     CDS             complement(10841..12946)
                     /locus_tag="WHT44_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HEZ2532375.1"
                     /translation="MKTDIQTELTQALLPHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLSNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSVNNSYTKYANRIGLTDSNRFL
                     KDSSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRNNSQPASQPASQPASQPASQPASQP
                     ASQLYTKLTRKRQEIFFNQTLAFDEIDRLFDAKAFSKFSRYTADGKQAVGEIKRHSDG
                     TPAENLIIKGNNLIALHSLAKQFKGKVKLIYIDPPYNTGNDGFKYNDKFNHSTWLTFM
                     KNRLEIAKELLMKDGSIFVSIDDNEQAYLKILMDEVFGNENFICNFIWEKKTGASDAK
                     QIATITEFVLCYSKNFKTVKLNKNTFSYDTERYKLSDKFEQERGKYYIDNLDRGGLQY
                     SDSLNFAIQCPDGTFTYPNGRTEFVNDGWIWKWSKNKIDWAITNGFLEFRKSKSKKSG
                     WSVCYKNYMLVDNENKPIERSAPYKNLIQDILNTHATDELKKLFGSKVFTTPKPESLL
                     QYLIQIATSESDIVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIETLAVERLKKVIDG
                     EQGGISKAVNWQGGGEFVYAELAPFNETAKQQILACEDSDGIKTLFEGLCERYFLKYN
                     VSVNEFSQIIQEPEFQSLPLDEQKQMVLEMLDLNQMYVSLSEMDDEQFADCLNDDDKA
                     LSRAFYQSVKNQAEKKDGE"
     gene            complement(13163..14086)
                     /gene="truB"
                     /locus_tag="WHT44_000133"
     CDS             complement(13163..14086)
                     /gene="truB"
                     /locus_tag="WHT44_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ2532376.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIATARAD
                     ISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARYITVYAIDI
                     AEFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLNETERDSLLLPCDVLVSHFPQTVLNDYAVHMLHCGQRPRFEEDLPSDTPVRVYT
                     ENGRFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            complement(14119..14490)
                     /gene="rbfA"
                     /locus_tag="WHT44_000134"
     CDS             complement(14119..14490)
                     /gene="rbfA"
                     /locus_tag="WHT44_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ2532377.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKTPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            14681..15925
                     /gene="clpX"
                     /locus_tag="WHT44_000135"
     CDS             14681..15925
                     /gene="clpX"
                     /locus_tag="WHT44_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ2532378.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLKGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            complement(16028..17224)
                     /locus_tag="WHT44_000136"
     CDS             complement(16028..17224)
                     /locus_tag="WHT44_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ2532379.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTKGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGSEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINTPETLNHVREQGQKLQTALLDLCRKTGLFSQVRGMGLLLGCVL
                     DEAYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDMAEGLRRLEHALTEFA
                     ATSDNP"
     gene            17628..18128
                     /locus_tag="WHT44_000137"
     CDS             17628..18128
                     /locus_tag="WHT44_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532380.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            18213..18767
                     /locus_tag="WHT44_000138"
     CDS             18213..18767
                     /locus_tag="WHT44_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532381.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            18781..18981
                     /locus_tag="WHT44_000139"
     CDS             18781..18981
                     /locus_tag="WHT44_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532382.1"
                     /translation="MPSEELSDGIFAFVRDIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
     gene            complement(19256..20644)
                     /locus_tag="WHT44_000140"
     CDS             complement(19256..20644)
                     /locus_tag="WHT44_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ2532383.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQKLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPSKEGFGGKTRGEGFKKEGFKRDGFKKTGEGFKGKRKAGDS
                     FAGKGERRYKDR"
     gene            20972..21919
                     /locus_tag="WHT44_000141"
     CDS             20972..21919
                     /locus_tag="WHT44_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ2532384.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWAKRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            complement(21972..22304)
                     /gene="trxA"
                     /locus_tag="WHT44_000142"
     CDS             complement(21972..22304)
                     /gene="trxA"
                     /locus_tag="WHT44_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ2532385.1"
                     /translation="MSSELIVHTSDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            22602..23075
                     /locus_tag="WHT44_000143"
     CDS             22602..23075
                     /locus_tag="WHT44_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ2532386.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAELLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPIGVMMHEHEEHD
                     RAIARLKELTDNFQPPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            complement(23145..23966)
                     /gene="nadE"
                     /locus_tag="WHT44_000144"
     CDS             complement(23145..23966)
                     /gene="nadE"
                     /locus_tag="WHT44_000144"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ2532387.1"
                     /translation="MMRIFSTGGKMDTQAVITHIARWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRPTLLLDMPIRQHPGQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGAHQTAFDSQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            24163..25518
                     /locus_tag="WHT44_000145"
     CDS             24163..25518
                     /locus_tag="WHT44_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ2532388.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSLLENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAVRLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIESCTVPVVSGVGHETDFTLADFVADMRAPTPTGAAELVSPNRQESLHRLAQ
                     AQCRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMTQNVRT
                     HTARFERQTQALKHCRPDVSVCKKNIDRFQTALSHAFHQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHIIFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            25811..27352
                     /locus_tag="WHT44_000146"
     CDS             25811..27352
                     /locus_tag="WHT44_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ2532389.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNTFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSIGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMACLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(27421..28470)
                     /locus_tag="WHT44_000147"
     CDS             complement(27421..28470)
                     /locus_tag="WHT44_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ2532390.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETGKTASQPY
                     GQKASDGIKPQNVPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWITNGWITVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRIGFGPIGLPNRLKRGQFYELNPAEVANIIK
                     WADMLLPGERRRKKA"
     gene            complement(28479..28706)
                     /locus_tag="WHT44_000148"
     CDS             complement(28479..28706)
                     /locus_tag="WHT44_000148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532391.1"
                     /translation="MQIHLLLLLSFERFYSRTQTDFNRRTNFAPTPSRSDDFIRKTAA
                     SGTISNRPTEDGILSTRPAAHAAEEPPYQAS"
     gene            28695..29327
                     /gene="pdxH"
                     /locus_tag="WHT44_000149"
     CDS             28695..29327
                     /gene="pdxH"
                     /locus_tag="WHT44_000149"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ2532392.1"
                     /translation="MDLHNIREDYSKRELSEADCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLLEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            complement(29457..30467)
                     /locus_tag="WHT44_000150"
     CDS             complement(29457..30467)
                     /locus_tag="WHT44_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ2532393.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLTAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMGGHSEQALSEAEKAQGKILMCCTTAQSDININIPGYKADALPVRTLP
                     ARIESIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANLPDQEGILE
                     LHIRRHENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQGSNRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRNGHVQDIAAQDHPDLSEYEVFACGSPAMTEQTKNLFVQQHKLPENLFFSDAFTPS
                     AS"
BASE COUNT         6835 a         7429 c         8120 g         8108 t
ORIGIN      
        1 tgtttttaat tgactataat ccgctatatt gtgagaagct ggatgacgga tgagggataa
       61 aataaatgcc gtctgaatct tcagacggca tcgggagatg attttcagtt ctcgacttcc
      121 ggttcggtta cattggaaat tacagaagcg tcggagagtt cggattcgtc ttcggcaaca
      181 cgttccagcg ataccaaggt ttcgccttcg tccaagttaa tcagtttcac gcctgctgcg
      241 gcgcggccgg tttcgcggat ttgttcgact ttggtgcgga taagtacgcc gccgctggta
      301 atcagcatca aatcgtcggt ttcgccgacc aaggttgcgg cgaccaaatc gccgtttcgc
      361 tcgccagtgt taatggcaat attgccttgc ccgcctttgt ttttgcggct gtaatcggca
      421 atcggggtgc gttttccgta tccgttggcg gtggcggtta aaacttgcaa accgctttct
      481 tcggtttcag gggcgaaggt aatcaggctg acgattttgc cgtcggcagg caggcgcata
      541 ccgcgcaaac cgccgctgcc gcgaccggac gggcgaacac cgtgtttgcc gctcggcagt
      601 gcgttttcgc tgtcggcggt ttcatcttcg atgccgtctg aaatttcggt ttcgatgtcg
      661 gcatcttccg cttcgtcgtt gccggatttt tcccagtatt cgttgaagcg gatggcttta
      721 cctaagttgg agaacagcat gatgtcgtcc gcaccgcctg tttgcgcagc gccgacgagg
      781 tagtcgcctt ctttgagcgc gatggcttta atgccttggg cgcggacgtt tttaaaggcg
      841 gaaagttgga cttttttcac cattccctgc gcggtggcga agaagacgta ttggtcttcg
      901 gggaactcgc gtactgccag aatcgcgctg actttttcgc cttcttccaa ctggatgacg
      961 ttgttaatcg gacggccgcg gctgttgcgt ccgccttcgg gcagtttgta aaccttaatc
     1021 caatgacact tgcccaaatt ggtaaagcac atcaaataat catgcgtgtt ggcaacaaac
     1081 agggtttcga taaagtcttc gtctttggtg gcagccgcct gtttgccgcg cccgccgcga
     1141 cgctgcgcct gatagtcggt ggtcggctgg gttttgatat agccgccatg tgtcagggta
     1201 acgaccattt cgcgttgcgg aatcaggtct tcatcggcaa tgtcgccgcc gaacgggttg
     1261 atttcgctgc ggcgttcgtc gccatagttg gttttgattt cttccagttc gtcgcggatg
     1321 atttgggtaa tgcgttcggg tttggagagg atatccacaa agtcgatgat tttacccatc
     1381 aggtttttgt agctttcgac aatttcttct tgatcgaggc cggtcaggtt tcgcaggctc
     1441 atgcgtaaaa tagcatctgc ctgaatctcg ctcaggtaat aaccttgctc tttcaagccg
     1501 atgtttgcag ccaatccttc cggacgcatc atttccaaat ccagaccgga acgcgtcagc
     1561 atttcttcaa cgaggctgct gcgccaaggg cgcgcaagca gtttgtcttt ggcctcggct
     1621 gcgttgggcg attctttgat gagcttgatg atttcatcga tattggacag tgcgacggct
     1681 ttgccttcgg caatatgccc ttcatggcgt gccttcttca gccggaaaag cgtacgtcgg
     1741 gtaacgactt cgcggcggtg gcgcaggaat tcggagagaa tctgtttcag gttcaacagg
     1801 cgcggttgtc cgtcgaccaa aaccaccata ttgatgccga aactgtcttg cagcggagtc
     1861 agtttgtaga gttggtttaa gacgacttcg gcattttcgt tgcgtttcag ctcgataacg
     1921 acgcgcatac cggatttgtc ggattcgtcg cggagctcgg aaatgccttc cagtgttttt
     1981 tcccgaacca aatcgccgat tttctcgacc agcttggctt tgttgacctg ataggggatt
     2041 tcgtcgataa cgatggcttc gcgttcgccg tttctgccta tgggttcgat atgggtctta
     2101 ccgcgcataa cgacgcggcc gcggcctgtt ttatagcctt cgcgcacgcc gctcaagccg
     2161 tagatggttg ccccggtcgg gaagtcgggg gcttggataa tgtcgatcag ttcgtcgatt
     2221 tcggtgtcgg gtgcatcgag caggcgcagg caggcattga cggtatcaga aaggttgtgc
     2281 ggcgggatat tggtcgccat gccgacggcg atgccggacg agccgttgac gagcagtgtg
     2341 gggaaacggg tcggcagtac aagcggctcg tgttcgctac cgtcgtagtt cgggccgaaa
     2401 ttgacggttt cttcctcaat gtctgccagc atttcgtggg aaattttcgc catgcggatt
     2461 tcggtgtagc gcatggctgc ggcggcaagc ccgtccaccg atccgaagtt gccctgtccg
     2521 tctatcagca cataacgcat agcgaaattt tgcgccatac ggacgatggt gtcgtatacg
     2581 gcggtatcgc cgtgggggtg gtatttaccg atgacgtcgc cgacaatgcg cgccgatttt
     2641 ttgtaggcgg cattccagtt gtttttcagc tcgtgcatcg cgtacagtac gcggcggtgt
     2701 accggcttga gaccgtcgcg aacgtccggc agcgcgcgcc cgacaatgac gctcatggcg
     2761 tagtcgagat agcttttgcg catttcgtct tcaaggctta ccggcagggt ttcgagggcg
     2821 aatttgtggt cgtggcggat ggttgcgtcg gtcatggttt caatgtttcg tatggcaaaa
     2881 aattgttgct tattttagca tattttgacg cggaacggtg cggcggttac gccgtctgaa
     2941 acacggtgcg gattataatg ccgaggaaat ttcgttgcgg agtttgtcga gaaacctgcc
     3001 ttggcggact tgttgtgcgg ctgtgtcgta atcttgtcgg gcaaacgact gtttcagacc
     3061 gcagaacagt ttttcttgtt cgtcgcggat ttcgttgtcg agatttttca aggattcaag
     3121 gtcgttgcct gcccgtgcct ccatcagcgt ttcgcgccat tccatttgct gcataaggaa
     3181 ttcgggggcg aaagcggtat gctccggcgc gtcggcatcg atgcccgatg ttttcagcag
     3241 gtaggcggcg cggtcgatgg ggtttttcaa ggtgcggtag gcatcgttga tggtggaaga
     3301 catcatcact gcctgctttt gctcaaaggc ggaagctgaa gcgaatttat cgggatggaa
     3361 acgggcggcc aaggcgcggt aggtttgttc caagttttcg gtgtcgatat cgaaagcggg
     3421 ttcaatccgg aagagggtga aatattggga catagtagga tgataaatgt aagattttgg
     3481 cagaaaactg tttttgcctt ataatctgcc gcttcttaaa cgaaaggact gaatatgggc
     3541 ggcaaagtgc agcacaataa aggcaaaata cgcgacaatg ctttaaaagc cttagtgaaa
     3601 tccgatttgt tccggcacaa ggtggaacgg aaaaggaaag gcaaaggcag ctacaacagg
     3661 caggaagcga aaaaatggcg ggacggtttt gatacggtcc cgccgttttt atgccttaaa
     3721 cgtggaagct ttcgccgcag ccgcaggagt ctttgacatt ggggttttca aatttgaaac
     3781 cttcctgcaa accttctttg gtgtaatcga cttgcgtgcc atccagataa accaggcttt
     3841 tcggatcgat ataaatgcgc gcgccgtgtc cttcgaaaat caggtcgtcg ccatcggctt
     3901 cgtcgacaaa ttcaaggttg tacgccatcc ccgagcagcc gctggttttc acacccaagc
     3961 gtacgcccaa gcctttgccg cgtttggcga gatagtcatt gatgtgtttt gcggcattct
     4021 cggtaagggt aatcatattt cttccttgtt gtaccgcccc ggacggacct gaagcggcgg
     4081 gtggttcgga cggcattgcg ggatgatgcc gtctgaaggg ctttatctgt tttcctgacg
     4141 tttgcggtag tcggcaacgg ccgcttttac cgcatcttca gccaagatgg agcagtggat
     4201 ttttaccggc ggcaattcca actcctcggc gatttcgctg tttttgattg ccagcgcgtc
     4261 atccaggctt ttgcctttaa cccactcggt aatcaggctg gacgaagcga tggccgagcc
     4321 gcagccgtaa gttttaaatt tcgcatcttc gatgatgccc tcgtcgttca ctttgatttg
     4381 caggcgcatg acgtcgccgc aggcgggcgc gccgaccatg ccggtgccga cggaatcgtc
     4441 tcccttgtcg aatgtgccga cgttgcgcgg attttcatag tggtcgatta ctttatcgct
     4501 gtatgccatg atgtggtttc cttaatgttt tttgatggtt taagtggttt gtttgccgat
     4561 tttcagacgg cctgaagttt agattttgca cgccccgcct tcgcagccgt cgtcgttttc
     4621 cgaatcgacg ccgccttcca cggtaacgcc gtcaaaatct tcaagcaggt tgtcgagatt
     4681 gtccaaatct aaatcttgtt cgttcatatt ttctcttgat tgctttgttt ttggaatgcc
     4741 gtctaaaggt tcggacggca tgttggtacg ctttaatgcg ctgcccattc aatcgagttc
     4801 aaatcaatcc cgtctttgaa catttcccac agcggcgaca gttcgcgcag tttgccgatt
     4861 ttggatttaa tcagttcggc ggcgaattgc acttcttctt cggtggtcat gcgaccgaag
     4921 gtgatgcgca gggatgagtg cgccagttca tcgttgcggc cgagcgcgcg caggacgtag
     4981 ctgggttcga gcgaggcgga ggtgcaggcg gagccgctgg atacggcgag ttctttcact
     5041 gccataatca ggctttcgcc ttcgacgaag ttgaagctga cgtttaggtt gttcgggacg
     5101 cgatgttcga ggtcgccgtt gatatagact tcttcgatgc cttcgatacc tttgaggaaa
     5161 atatcgcgca gtttcaggta gtgtgcagtg tcttgtgcca attcttcttt ggcaatgcgg
     5221 aaggcttcac ccatgccgac gatttgatgg gtcggcaatg tgccggaacg gaaaccgcgt
     5281 tcgtgaccgc cgccgtgcat ttgggcttcg aggcggacgc gtggtttacg gcgtacatac
     5341 agggcgccga tgcctttagg gccgtatact ttgtggccgg acatagacag caaatcaact
     5401 tttgcggctt caacatcaac aggcactttg ccgcatgctt gtgctgcgtc aacgtggaaa
     5461 atgattttgc gttcgcggca gatttcgccg atggcaggaa tatcttgaac cacgccgatt
     5521 tcgttgttta cccacattac ggaaacgagg atggtgtctt cgcggatggc ggctttcagt
     5581 acgtctaaat caaccaaacc gttttcttgt acgtccagat aagttacttc gtaaccttgg
     5641 cgttcgagtt cgcgcatggt gtcgagtacg gctttgtgtt cggtttttac agtgatgagg
     5701 tgattacctt tagatttgta gaagtgcgcc gcgcctttga tagcgaggtt gttggactcg
     5761 gttgcgccgc tggtgaaaac gatttcttta gagtcggcgt taatcagggc ggcaatgtct
     5821 gcacgtgctt tttctacagc ttcttctgct tcccagccga agctgtggct gttggaggct
     5881 gggttgccga aggtttcggt cagataggga atcatttttt cggcaacgcg tttgtcaacg
     5941 ggggtggtgg cggcgtagtc gaggtaaacg ggggttttga cggtcatggt ttgctctttc
     6001 tttttcgggt gttatttaat ggatgtgtgt aaattggacg acgcggctgc cgtcgccgtt
     6061 gtttttctgt tcgatgatgc tttgcagggt aacgctgccg aggtagtcgt tgatggtttt
     6121 gtttaaattc tcccaaagat cgtgcgtcag gcagggcgcg ccgtggtggc agttggcttt
     6181 gctgccgcat tgggttgcgt ccagccggtc ttcggcggcg gcgatgattt gggcgatgtt
     6241 gatgcgtgcc gccggtgcgg cgaggatgta gccgccgccg ggcccgcgca ggctttcaac
     6301 aagtccggcg cggcggagtt tgccgaacaa ttgctcgaga taggagaggg atatgttttg
     6361 gcgttcgctg atggcactga gtttgacggc gccggtttgc gcgttcatcg ccaaatccag
     6421 catagcggta acggcgaaac gccctttggt ggtcagtctc atggtggtgg ttgcccatgt
     6481 cggttttttt attagtgggt ggaattgtcc aatatctgag tgtttcagtc aagtatgagg
     6541 gcggattgtg tgattggctg tttaaatcga tagattgatt gatttttaat attttgattt
     6601 taatttgtta tttttgaatt ttaaacctta tgtcgcttaa agtagggagc tgcggcggat
     6661 gcggcaaatg ttgaaatgat ttgccttata atcaatattg atatacatta tcgatttcat
     6721 ataaagttat tcaggtattc agggggcgac aaatgaaacg tgatttgagc aaaatgacct
     6781 gcatcgaaga cttgcgccgc gttgccaagc gcaaaatgcc gcgtatgttt tacgattaca
     6841 tcgattcggg ttcgtggaca gaaactacct accgtgaaaa cacttcggat ttcaaagaca
     6901 tccgcttccg tcaaaaagta ttggtcaata tggaaggccg aagcctggaa accaaaatga
     6961 tcgggcagga tgtgaaaatg ccggtggcga ttgcgccgac gggtttcacg ggtatggcac
     7021 acgccgacgg tgaaattttg gcggcgcggg cggcggagaa gttcggcatt ccgtttacgc
     7081 tgtccacgat gtctatctgc tcgattgaag acgttgccga gaacacttcc gcaccatttt
     7141 ggtttcagct ttatgtgatg cgcgaccgcg agtttatgga aaacctgatt aagcgcgcga
     7201 aggatgccaa atgttcggca ttggtattga ccgctgattt gcaggttttg gggcaacgcc
     7261 acaaagacat caaaaacggc ctgtccgcgc cgccgaaacc gaccatcgcc aatttaatca
     7321 atctggcgac caagcccgaa tggtgtatga aaatgctgaa cacgcaacgc cgcacgttcc
     7381 gcaatatcgt cggacacgcg aagaacgtcg gcgatttgtc ttcgctgtct tcatggactt
     7441 ccgaacaatt cgacccgcgc ctgagctggg acgatgtggc gcgcattaaa gatttgtggg
     7501 gcggcaagct gattatcaaa ggcattatgg aacctgaaga cgcggaaaaa gcagcgaaaa
     7561 gcggcgcgga cgcattgatt gtttccaacc acggcggtcg acagctcgac gataccgtgt
     7621 ctgccatcaa agccttgccc gacatcgtca gcgcagtggg cagcgacatc gaagtttgga
     7681 tggacagcgg catccgcagc ggtcaggata ttttgaaggc gtgggctttg ggcgcgaaag
     7741 gtacaatgat aggtcgggcg ttcctgtatg gcttgggcgc gtatggagaa gaaggcgtaa
     7801 cccgtgcgct ggaaatcctg tataaagaaa tggatatatc tatggcgttt acgggacacc
     7861 gcgatattca agatgtcgat gcttcgattt tgagaggcaa agattggggc agggaaacgg
     7921 tttagatact gaaaaatacg atgccgtctg aacacagggt tcggacggca ttaaaatttg
     7981 gctgtatttt aataatgtgt tgattaaata tctgacaaaa cgcccttttg taaaggaagt
     8041 tttgtcaaac ggggttgcct gcaatgcacc actctatttt tcaagcgatg ccgcccctaa
     8101 agggaatgac tttgtaaatt gttcattttc ctgattgtca gtaaaaaacg gcaaaccgat
     8161 caaacgataa tgcggtgtat ctttttgcag aatcttatcc gtcccgtatt ccgctgtaat
     8221 tgcctttaaa aaagcgtctt tccacgaatc attttccacc aaatgctcac cttttggttc
     8281 aatgaaaatt tggtaatgca aaaagtcatc cacaccattg gaagaagatt tttgtttatt
     8341 tttcagcaat aaaataaagt ccggcataaa tccttcacca tcggaaaagt tattcaattt
     8401 aaacacttct tcattacgga ttaaatgaac atcatattta gacttcaaat cgcccaaatg
     8461 ctctgaaata aattgaatta acgcttcttc caaactcgtt ccagcaaaat tatccatcac
     8521 ataccaatca ttttgcgtgg caatggcagt ttttatatca tcttttttga cccatttttg
     8581 ttttggtgtg ccaaaaattt cccacaattt tttaggcgta aattcttttg taccgataaa
     8641 tggcatatca ctttcattca aatgtttttc aaccatttcc aagattttta ggcagcctgc
     8701 aagtttgtca tctgggctga tctgtttgtc ttgccctaat cccaaaaatt caatttgtca
     8761 atcttttaac aagttatttt gcaattcatt gcgattgtaa atgttgagtt tgctttgcaa
     8821 gcggtcaaaa tggaataaag attgaccatt ttttcctttg atatgcaggg atttattgaa
     8881 aatgtgccgt tccatttcac tcatttttat gatttgagta aaattatttt gtgtgtcgat
     8941 ttgtcgggct atttcatcat tttcatcggc tgtaaattgc gtttcctgca acagttgatt
     9001 gccgtgaact tggaatggaa gcgtttgcgg attggctttc aggctgtctg cattattggc
     9061 tctggcattg ggattgggga tttttttatt tgcccaaatt aacaactctc taaaatcctg
     9121 attatcggca aattcagatt tgagtttaaa tgttgccaat accttatcat cgtctttttc
     9181 aggcaaataa ccgtcttttc gtaactcgtt tttcagttct gtaatatagc gagattgctc
     9241 atcgtgcgtg taataaaaca attcttccaa aatacgcaat tcgtgttgca tatcgttgtc
     9301 aaatttgcgt ttattcggct gtttaccttc aaacgcaaat ggaaaataac gcacgccgcg
     9361 accaatcaac tgcttttccg atacagtagc ggcagccgtt ttgcctgatt ttttatttga
     9421 accgccgccg ttttgccctt catacaaacg cacaatatca aacaaattca gaacgtccca
     9481 accttcggtt aatctgtcca ccgtaaaaat ggcacgaatc ggattatcag ccgcttccaa
     9541 attattcagc aatttttctg tttcgctgtc ggttttttcg gttttggttt tgttggtttc
     9601 ggagttggta ataatcacat tgtgtttttg ataattttgt tttacccaat ctgccaaatg
     9661 tgcaaactca acgccttttt cctgcataaa ttttagggct tgttcagtgc gggttttgcc
     9721 ttgttcgttg gcgttatcgc tatcgctatc gttcaagctt gttgaaaatg tagttaaaaa
     9781 cgaaaaatca accgcctgca cattttctgc ccaatttaaa aatgccagat aatccgcttt
     9841 tgattcatca atcgtcttac ttctaaacaa catcacaggc ttgaaattgg caatgccata
     9901 tttcaacgca attcgatgtc gataccaagc aaacaataaa gcgtgtaaca ctcgctcttt
     9961 cttacccagc gtactggata ccaaattgat ttctttggta taaccttttt gcaaaaattc
    10021 ttttaagcca aattttgtga tgattttatc agcgtatttt tgttgtacat cggcattttc
    10081 aggcagcgtg gcggtaaatt ccaacagcac attttggcta tgattgccat ttttattgag
    10141 taacaattcc aaaaccatat gctcccagcc tttacgttca atttcggcat tgctggtgcg
    10201 gtcgttcatt tccttttcta aatctaattc gccttgtttt ttgcctttgg tttgcgcgtt
    10261 taaatggtgc gcttcatcac ccagcatcac aaggttcaat ttgtgcaaat ccgccaatgt
    10321 ggtttgattt tcccgccggg tgcgaatatc gttatacagc ttttgaatgc tggtaaattt
    10381 aatttcaatg ccgtctgaat gtgggctaaa tgtctccact ttgcgaatag gaattaccgt
    10441 atcgccctgc aaaatcttct cggtaaataa aaattttgcg tgcgtcggat cggtaaaatt
    10501 attttccgtt ttatccacga tattgttttg attcacaaaa aacagaaaat gccgataacc
    10561 tttttcaaaa taatacaaaa tcaacgccgc catcatcatc gttttgccag cacctgttgc
    10621 catattgaac agcaaatgcg ttggcttatt ttttaaatca gggaaatcgt ctagctttga
    10681 agtgcggtca aaaatcaaaa agttttcaag ggcagatttt tgccattcaa aaaaatcata
    10741 ttttaagttt ttggacgtaa attctggcat ttcaagtaat tcaggcttgt attttttaac
    10801 cgctttacgt tcattttcaa tcacttcaaa caacgtttta ttattcgcca tcttttttct
    10861 ccgcttgatt ttttactgat tgatagaatg cacggcttaa ggctttatca tcatcgttca
    10921 ggcaatctgc aaattgttcg tcatccattt cggataatga aacatacatt tgatttaaat
    10981 ccaacatttc aagcaccatt tgtttttgtt cgtctaatgg caaagattga aattcaggct
    11041 cttgaatgat ttgactaaat tcatttacgc tgacgttgta tttcaagaaa tagcgttcgc
    11101 ataaaccttc aaacagcgtt ttgatgccgt ctgaatcttc gcaagccaaa atttgttgtt
    11161 ttgcggtttc gttaaatggg gcaagttcgg cataaacaaa ctcgccacca ccttgccaat
    11221 tcacggcttt ggaaataccg ccttgctcgc catcaatcac ttttttcaag cgttcaacag
    11281 caagcgtttc aatataatcc atttgttcaa taccgatata ttggcggttc attttgtggg
    11341 caacggcggc ggttgtgcca ctaccaagat ggtagtctaa gacgatgtcg gattcggatg
    11401 tggcaatttg aataagatac tgcaataagc tctcaggttt tggagtagta aaaactttgc
    11461 tgccgaacag ttttttcaat tcatctgtcg catgtgtatt taagatatcc tgtattaagt
    11521 tcttataggg agcagaacgt tctatcggct tgttttcgtt atcaaccaac atatagtttt
    11581 tataacatac actccatcca gattttttag actttgattt tctaaactcc aaaaaaccgt
    11641 ttgttattgc ccaatcaatt ttatttttac tccatttcca tatccagcca tcattgacaa
    11701 attcagtcct gccattcgga tacgtaaaag tgccatctgg acattggatt gcaaaattca
    11761 aactgtcact atactgcaat ccccctctat ctaaattgtc gatataatat ttgcctcttt
    11821 cctgttcaaa cttatcactt aatttgtatc tctctgtatc ataagaaaac gtgtttttat
    11881 ttaatttaac tgttttaaag ttctttgagt aacataagac aaactctgta atagtcgcta
    11941 tctgtttggc atcggacgca cctgtctttt tttcccaaat aaaattgcag atgaaatttt
    12001 catttccgaa aacttcatcc attaaaattt tcaaatatgc ctgttcgttg tcgtcaattg
    12061 acacaaaaat cgaaccgtct ttcataagca gctctttggc gatttctaga cggtttttca
    12121 taaaagtcag ccaagtggaa tgattaaatt tgtcgttgta tttaaaaccg tcattacccg
    12181 tgttatatgg cgggtcaata taaatcagct tcactttgcc tttaaactgc ttggcaagcg
    12241 aatgcagggc aatcagatta ttgcctttga taatgagatt ttcggcgggt gtgccgtctg
    12301 aatgtcgttt gatttcgcca acggcttgtt tgccgtctgc ggtatagcga gagaattttg
    12361 agaatgcttt tgcgtcaaaa agccgatcaa tttcatcaaa agcaagggtt tgattaaaaa
    12421 agatttcttg tctttttcgg gttaatttag tgtataattg gctggctggc tggctggctg
    12481 gctggctggc tggctggctg gctggctggc tggctggctg gctggctggc tggctgttat
    12541 tgcgtttaaa ataaatttct tcgccttcct cggtgctttg tccgccattt aacacacaat
    12601 ctttaaacgg aaaatccaac acaatatcac tgctgtcttt caaaaagcgg ttgctgtccg
    12661 tcaaaccaat gcggttggcg tattttgtgt aggaattatt gacgctgtgt ttgtccaaga
    12721 aaaaacggaa atcctgcaat ttaaacacca gcacaccatt cacttccaca aagaaatggc
    12781 gttttaactc atcattactc aataacaaac cgataatggt cgggtcggtt ttttccacca
    12841 aatccaacag gatgtttttg gctaaaatgg ttttttcttc gttcgcccat actttttcgt
    12901 gtggtagtag ggcttgggtt aattcggttt gaatgtctgt cttcatcgtt ataaatcctc
    12961 atcagttatt ttaatgtgcc attcttgtgt aacggcaaat ggctttgcaa atagattcta
    13021 ataaaatcaa ttatttgatg tctttgctgt ttccgggggc gttcgcaaag ttgcgataag
    13081 cggggcgggt gtccgagtgt gtcgggattt tgcggaaata tcaacacatt attcaagcac
    13141 agcctgtatt tttaaccgcc gttcaggcgg cggatgccgc cgtgttcatc aggcgcaagg
    13201 ctttcagacg gcatatttct ttttgatatt ccgccagacc gacaaagcgg ccgttttccg
    13261 tgtaaacgcg taccggcgtg tcggaaggca ggtcttcttc gaaacgcgga cgttgtccgc
    13321 agtggagcat atggacggca taatcgttta aaacggtttg gggaaagtgt gaaaccaata
    13381 cgtcgcaggg tagcagcaag ctgtcgcgtt ctgtttcgtt caaatttgcc aaggcctcaa
    13441 gcgtgtggct ttgggcgatg gtaaagccgg cggtttcagt gcggcgcagg gcggtcaggt
    13501 gggcgaacgt gccgatgtgt ttggcgatgt cttcgctgag ggtgcggatg taggtgcctt
    13561 tgctgcaacg tacgtcgatg acggctttgg gcgcgtcaaa ttcggcaata tcgatggcgt
    13621 aaacggtaat gtagcgcgct ttgcgttcga tgacgatgcc tttgcgggcg tattcgtaca
    13681 gcggtttgcc ttcgtgcttg agcgcggaaa acattggcgg cacttggcgg atgttgcctg
    13741 tcagtgcttg gcaggccgtc tgaaattcgg ctaaggaaat atcggcgcgg gcggtggcaa
    13801 tgatttcgcc ttcggcatca cccgtgctgc tggcttcgcc gagtttcagc gtggcggtgt
    13861 aggctttgtc ggcatccagc aggtattggg cgaacttggt cgcttcaccg aagcagacgg
    13921 gcaaaagtcc ggttgccaaa gggtcgagca cgccggtatg tccggctttt tcggcatgaa
    13981 acaaacgccg cgctttttgc agcgcggtgt tgctggaaag gccttcgggt ttgtccaaga
    14041 gcaaaacacc gttgacggca cgtttttggg gtttgcctgt attcatggtt cgatgttcgg
    14101 acggcatggg catatccgtc agtcttcaac cggtttttcc gccgctactt ggtcgataag
    14161 ggcggacagg ttcaaaccgc gttccaaaga ttcgtcgtat ttgaaatgca gttcgggcgt
    14221 tttgaacagc ttgatgcgtt tggccaattc gctgcggagg tgtccgcgcg cgtgttccag
    14281 cacttcttcc gtaatttcgc gcgcgtcttg gtttaaaatg gtgtagaaca cggtggcgtg
    14341 gctgtaatcg cgggtaactt cgacttcgtt gacggtaatg aagccggcgc gcgggtcttt
    14401 cagtccggta cggacgagtt cggcaagctc gcgcataatt tgttctttga cacggtcttg
    14461 gcgggcatag ccgcgttggg gttttctcat gaatatcctc agatttcaga tttggtttca
    14521 gacggcattt ttgccgccgg ggcggttgcg aaagggcgta ttatagccta atcggcggga
    14581 ttttaaggta atacattgat aagaacggat atagttatta tacttggcaa cccgaacggc
    14641 acgtgccgtt ttctgtaacg catacattgt aaagaaagcc atgtccaacg aaaaccgtac
    14701 ttgttccttt tgcggaaaat ccaaatcaca cgtcaaacat ttaattgagg gcgaaaacgc
    14761 ctttatctgt gacgaatgcg tctcaaactg tattgaaata ttgcatgaag acggaaatga
    14821 cggcacgcct tcggaaagtg ccggagggga gccggaagaa tccggcaagc tgcccacgcc
    14881 cgccgaaatc gtcgccaacc tcaacgacca cgttatcggt caggaacaag ccaagaaagc
    14941 cttggcggtg tcggtgtaca accattacaa acgcctgcgc catcctaaag caggagctaa
    15001 tgtcgaattg tcgaaatcca acatcctgct tatcggcccg accggatcgg gtaaaaccct
    15061 gctggcgcaa tctttggcgc gcaaactgga tgtgccgttc gtgatggcgg atgccacaac
    15121 tttgactgaa gcaggttatg tcggcgaaga cgtcgagcaa atcattacca agcttttggg
    15181 caaatgtgat ttcgatgtcg aaaaagccca gcgcggcatt gtctatatcg acgaaatcga
    15241 taaaatttca cgcaaaagcg acaacccgtc gattacccgc gacgtatccg gcgaaggtgt
    15301 gcaacaagcc ttgttgaaac tgattgaagg tacagtggcc agcgttccgc cccaaggcgg
    15361 ccgcaagcat ccgaatcagg aatttatcaa cgtcgatacc accaacatcc tgtttatctg
    15421 cggcggcgcg tttgcaggct tggaaaaagt catccgccag cgtactgaaa aaggcggtat
    15481 cggtttcggt gcgtccgttc acagcaagga cgaaaatgcc gacattacca agctgttcgg
    15541 catcgtcgaa ccggaagact tgattaaatt cggtctgatt ccagaattaa tcggacgttt
    15601 gcctgtgatt gcaactttag aagaattgga cgaagacgcg ctgattaata ttttgaccga
    15661 gccgaaaaat gctttggtca agcagtatca ggcgttgttc ggtatggaaa atgtcgaact
    15721 ggagtttgaa gaaggcgcat tgcgttccat cgcgcgtcag gcaatggaac gcaaaaccgg
    15781 cgcgcgcggc ctgaggtcca tcgtcgaacg ctgcctgctc gatacgatgt accgcctgcc
    15841 tgatttgaaa ggcttgaaaa aagtggtggt cggtaaggca gtcatcgaag agggcaggga
    15901 accggaattg gtgttcgagt cctgatggca aacaaaaccg cttcggaacg ttgaaccacg
    15961 tttctgaagc ggttttttgt ttctgatgcc gtctgaagcc ttcccgcctt cagacggcat
    16021 ttgagtttta cggattgtct gatgtcgcgg caaattccgt cagcgcgtgt tccaaacgtc
    16081 gcaaaccttc cgccatatcc tcatcgttca acagtagcga aggcgcgaaa cgcaatacgt
    16141 ccgcacccgc aaccaaaatc atcacgccgt gtttcaaggc ggcggcggtg atttcggatg
    16201 cgcgtccgcg ataggcttcg tccaacacgc agccgagtag cagccccatc ccgcgaactt
    16261 gtgagaacaa gcccgttttc ctgcacaaat ccagcaatgc cgtctgaagt ttctgcccct
    16321 gttcacggac atggtttaaa gtttcgggcg tattgatgat gtcgaatgcg cggctgccga
    16381 ccgcacacgc catcgggttg ccgccgaaag tcgagccgtg cgtgcccggt tggaaggcgg
    16441 cggcaatctt ttctgtcgcc agcatcgcgc cgatcggaaa gccgcagccc aaggcttttg
    16501 ccgaactcaa aatatcggga acaatgccgt aatgttcata ggcaaacagc ctgcccgtat
    16561 gccccatccc ggtttgaact tcgtccaaaa tcaacaacgc attgtgccgg tcgcacagac
    16621 ggcgcgcggt ttgcaaatat tccgcagtgg cgggcaggat gccgctttcg ccctgtatcg
    16681 gctcgatgat gaccgcgcag gtctgttcgc cgacggcagc ttccagcgcg gcaatatcgt
    16741 tgaacggaac gtgcgtaatg ccttgcggca ggggtgcata atccttgctg tatttcggct
    16801 gaccgccgac ggacacggta aacagcgtgc gtccgtgaaa actgttgata caggcgacga
    16861 tttcgctttt tcctccgccg aaacggtcgc gggcgtattt cctcgccagc ttcaacgccg
    16921 cctcattcgc ttccgagccc gagttgcaga aaaaaacctt gtcggcaaaa ctgtttgcaa
    16981 ccaatttttg cgccaattcc tgcgctggac gcgtcgtata gatattggaa atgtgccaca
    17041 gcttgtgcat ctgcgcgttt aaagcatcga caagggcagg gtggcagtgt cccagcgcat
    17101 tgacggcgat accgcctgaa aaatcaatat attcacgccc tttcgtatcc caaacgcggc
    17161 tgcctgaagc gcgttcggga atcatcgggg caaaggcgaa attgggggtc agatagtttt
    17221 gcataattgc tcctgattgc ggttttgctc gggattatgc accgtttttc aaagcagata
    17281 aagggaaatt gccgacaata tcgggcagcg gtaaaaataa accgaaatat tttcaatcgg
    17341 atagatgaaa aatcaaaaaa atttcgataa aatgcttgag gcgcggcagc gttttgattt
    17401 acaatctccc caatattttt atcttcagac ggcatttcat catgcaaggc aaaaaattta
    17461 caaccgctta ttgctattgg taccacacag acgtgcggta cttttttgcg cttgcttgaa
    17521 atccatccca tacgcaaaac aggccgctcg aaaacggggc ggcttttttg ttaccttcac
    17581 acaggtcgcc cgattcggca acacacgaca gaatcaggaa aatgaccatg aatgcaatgc
    17641 cccaaaccgc cgcacaaaac gaaaccctgc acacttgtag cattattgtc gatgatgcac
    17701 aggatttttc ccccatgctt ctcgatgcag ggcgggacga cacacttatc agcgaatcga
    17761 tgattccgca aatccgcacc gttgccgccc tgatagccgc cgaacggcac gacttcagcc
    17821 gcagcagccc cgccgagttt accgatgccg ccgatttttt tgccgcacgg attctcgtat
    17881 tgggcgtgcg acgcttccat ctggacgtat cgctgcttca gattttgaaa accgccaaca
    17941 gccgcgcccg ccgctttgcc gaaaaacacc gcctgttttt cacacccgca caagccgaat
    18001 tgagcctgaa caaacacaaa aaccgccgcc tgctgaccgt agaaaccgaa cacgaagtcg
    18061 aaaacaaagg caacccagtc gccaacagcc tcgcatttgt ccgcaaattg cacaccctgc
    18121 ccctgtaaac atcacgattt gccggcagaa tgcttgccaa aattcccgac ggcgatacaa
    18181 taaaaccgat tttccaccca aacaggacac acatgaaaaa aatcatcgcc tccgcgctta
    18241 tcgcaacatt cgcactcgcc gcctgccaag acgacacgca ggcgcggctc gaacagcagc
    18301 agaaacagat tgaagccctg caacagcagc tcgcacagca ggcagacgat acggtttacc
    18361 aactgactcc cgaagcagtc aaagacacca ttcctgccga agcacaggca aacggcaaca
    18421 acgggcaacc cgttaccggt aaagacgggc agcagtatat ttacgaccaa tcgacaggaa
    18481 gctggctgct gcaaagcctg gtcggcgcgg cggcaggcgc gtttatcggc aacgcgctgg
    18541 caaacaaatt cacacgggca ggcaaccaag acagtcccgt cgcccggcgc gcgcgtgcag
    18601 cctaccatca gtccgcacgc cccaatgcgc gcaccagcag ggatttgaac acgcgcagcc
    18661 tccgtgcaaa acaacaggcg gcgcaggcgc agcgttaccg cccgacaacg cgcccgcccg
    18721 ccaattaccg ccgccccgct atgcgcggtt tcggcaggag gcggtaaacc cggcgcgcca
    18781 atgccgtctg aagagctttc agacggcatt tttgcatttg ttagggacat tgttatgttg
    18841 ccgtttgatt ttcagacggc attttgtttc caagcgtttg atgtcgggat ggcaattctg
    18901 atttacgtgc gttggcaggg tggcattgtc atgccgccgt tgggttttca gacggcatca
    18961 tatcgaacag tcaagccgta gggcgagcat tcttgcccac cgttgcaagg gcgggattgg
    19021 gtgggcagga atgcccaccc tgcatctgaa acccttccgt gtcattcccg tgcaggaagg
    19081 aatctagata tttgaattta cggaaatttt caatcgttgc caaagcggaa aagggttttt
    19141 attacagcct aatcctttgg gtatcaaagc cgggcggtgc tgaaacattg caaaaatgcc
    19201 gtctgaaact cgaaatcggt ttcagacggc attggtttat gcggcaggtt ggggcttagc
    19261 ggtctttgta acggcgttcg cctttgcctg caaaagaatc gccggctttg cgtttgcctt
    19321 tgaagccttc gccggttttt ttgaaaccgt ctctcttaaa gccttctttc ttgaaacctt
    19381 cgccgcgcgt tttgccgccg aagccttctt tgctcggttt atgatcgccg cgccaaccgc
    19441 cggatttacg atcgccccag ccgcctttgc ctttcggctt gccgcctgcg gatttgcgtt
    19501 tgcgggtcgg ttccatgcct tcgatggtca gttcgggcag ttttcggtta atgtattttt
    19561 cgattttgtg gactttgacg tattcgttca cttcggcaaa cgtaatcgca atacccgtgc
    19621 ggcctgcgcg gccggtgcgc ccgatgcggt ggacgtagtc ttccgcctgt ttcggcaggt
    19681 cgtagttgat aacgtgggta atggtcggta cgtcgatacc gcgtgcggca acatcggtgg
    19741 caaccaaaat tttgcagcgg cctttacgca aatccatcag cgtgcggttg cgccagcctt
    19801 gcggcatatc gccgtgcagg cagtttgcgg cgaaaccttt ttcgtacagt tcatccgcaa
    19861 tgacttcggt catggctttg gtggacgtga aaatcacgca ttgatcgata ttggcatcgc
    19921 gcaagatatg atcgagcagg cggtttttgt ggcgcatatc gtcgcagtac agcagttgtt
    19981 cttcgatttt gccttgatcg tccacgcgtt cgacttcgat gatttcaggg tctttggtca
    20041 gtttgcgcgc cagtttgccg accgcgccgt cccaagtggc ggagaacaac aaagtctgac
    20101 ggtcgctcgg cgttgcttcc acgatggttt cgatgtcgtc gataaagccc atatccaaca
    20161 tacggtcggc ttcgtccaaa atcagcactt ccaaacgttc aaaatcaact ttgccgcttt
    20221 gcatcaggtc catcagacgg cccggcgtgg cgacaatcag atcgaccggt ttgctcaggg
    20281 cacgggtttg gtagccgaaa gacgcgccgc cgacgatgct gacggtgcgg aaccaacgca
    20341 tatttttggc atacgccagc gcgtttttct cgacttgagc cgccagttcg cgggtcgggg
    20401 tcaacaccaa agcacgcggg cctttgcccg gtttttcgct gcgtttggtc agtttttgca
    20461 aagtcggtaa cagaaaggcg gcggttttgc cggagccggt ttgcgccgaa gccatgatgt
    20521 cgcggccttc caaagcaaac ggaatggctt gcgcctgaat cggcgtcggg ctttcgtaac
    20581 cctcgctgct gacggcggac aaaatgtttt tatcaaggtt caaatcggca aatttaatag
    20641 acatggctat ccttggaaga caacaacgcc cgcgtccgaa aggttcagac ggcctgaagc
    20701 agggaagggt aacgatacgg ggaatgtgaa gttacagggt tgtcgcaagc ggcttgcttg
    20761 tggtttacat cgacggcaac ctgcacagat atgctttgca aaatcaaagc aacagaagaa
    20821 acgcgcccgt aagggcggtt taggttggaa ggcgatggct tcgtatgaca agataagcga
    20881 cggatggtat agggtttgcc cgaaacggtc aagcaatcgg gcgttaatta cagtaaaatt
    20941 gccgttttcc actttattcc gcctgaaacc aatgaccgac atcacaccgt ttgccaaccg
    21001 cttgggcaaa aacatcaaac accttatgaa atgggcgaaa cgcaacggca tcgaagcctg
    21061 gcgcatttac gaccgcgaca tcccccaatt cccctttgcc gccgatgttt acggcgatcg
    21121 aatccacctt caggaatacg ataccggctg gctgatgcgg cccgaagaat acgaagcgtg
    21181 gcttgccgaa gtcttggaag ccgtcgcttt cgttaccggt tttgcgcccg aacaaatccg
    21241 cctcaaacgc cgcgaacgcc aaaaaggttt gcagcagtac gagaaaaccg gcaaggcggg
    21301 cgacgatttc gtcatcaccg aaaacggccg caagttttgg gtcaaccttg ataagtattt
    21361 ggatacgggg ctttttttgg atcatcgaaa cacgcgcaaa aaagtcggcg aaacggcggc
    21421 gggcaaacgt tttttaaacc tgttttccta caccggcagc ttcaccgtct atgccgccac
    21481 cggcggcgcg gcatccagcg aaaccgtcga tttgtccaac acatatcttg attgggcgaa
    21541 acgcaatttt gaattgaacg gcatcgatac ggaacgacac aaaatcgtcc gcgccgacgt
    21601 gttccaatat cttcagacgg catatgggga aggcaggcgg ttcgacctca ttgtgatgga
    21661 cccgcccagt ttttccaaca gcaagaaaat gtccgacatc ctcgacatcc agcgcgacca
    21721 caaaaagctg attgacggcg cggtgaagct gctcgcttca gacggcattt tgtacttttc
    21781 caacaacctg cgcagcttcg tcttagacga cttggtatcg gaacaatacg ccgtcaaaga
    21841 catttccaaa caatccgttc ccgaagattt ccgcaacaaa aaaatccata ggtgctggga
    21901 aatccgccac aaatcttaag ggtgcgaaaa agccgccgga atcttccggc ggctttttcg
    21961 attgcgcttt atcaggcgat agaggcttcg acaaaggcgg tcagctgacc ttttgccaat
    22021 gcgccgactt tggtggcgac gacttcgccg tttttgaaca ccatcagggt cggaatgccg
    22081 cgcacgccga aacgggacgg ggtggcttcg ttgtcgtcga tgttgatttt gaccactttc
    22141 agacggcctt caaattcggc ggcaatgtcg tccaaaatcg gggcaatcat tttgcagggg
    22201 ccgcaccacg gagcccaaaa gtccagcagg acggggatat ctgcatttaa aacgtctttt
    22261 tcaaaagcgg catcgctggt gtgtacaatc aattcgctgc tcattatttt tccttttcgg
    22321 ttgggattgg cacgatagcg tgcaggatag ggcttgggca taaaaatttc aagtgggcgg
    22381 gcgggataat agtttttctg taccgcccca atcgcctttg caaattctaa aaaacggttc
    22441 ggacagtatt ttatttctga aaattacttt tattatcaaa atgtaaggaa tacgtttgag
    22501 gctgtacaga agcgcgcggg cggtgtatgc tccgtttgaa ttgattctta aagtatgaat
    22561 gttcaaaacc aaacccgaaa ccctgtaaaa ggaacactgc gatgactgat ttttccgttt
    22621 gggagaccgc cccctttggt gcaaccgtcg atcatatcct gcaacgctac cacaatgtcc
    22681 accgtgcaca atttgaagaa ttggtgccgc tggcgcaaaa agtggcccaa gttcatgccg
    22741 acacctttcc ggcggaaatt gccgagctgc ttgcctatat gcagaacgaa ctgctgatgc
    22801 atatgatgaa agaggaaagg atgctgtttc cgatgattaa tcagggtgtc ggacgcggtg
    22861 cggcgatgcc catcggcgtg atgatgcacg aacacgaaga acacgaccgc gccatcgcac
    22921 ggctcaaaga gctgaccgac aatttccaac cgccagaagg ggcttgcgga agctggacgc
    22981 ggctttacgc gctggcgaaa gaaatggtgg aggatttgaa cgaccacatt catttggaaa
    23041 acgatatttt gtttgcccgc gtactcgatt cttaagggat aggagtcgga tatttccatg
    23101 ccgtctgaaa tacggttttc cgtttcagac ggcatttccg tgtttcagcc cagcaattcg
    23161 ggcggaatgc ggcatacggg aatcgggttg attttgtgct gcatggcgcg gtgaagtcgc
    23221 gtatagattt ctagaacttc gcgctgccgc ccttcaaaat cttcgggttt gcgcgtgccg
    23281 tacacgccca ttgcccactc cagttcggga tagcttgcgc ccatctgttc ttcgtcggtg
    23341 cgttccgtat cccacaggcc gtcggtcggc ggggcttttt gaatcgcctc gtccacgccc
    23401 aatgcttcgg caagccggta aacctgcgtt ttggtcaggt cggcaatcgg gctgatgtcc
    23461 acgccgccgt cgccgtattt agtaaaaaag cccacgccga aatcttcaat cttattacct
    23521 gtccccgtaa ccagcagtcc gtgtatctgc ccgtagtagt acagggtcag catacgtagg
    23581 cggcttctgg cgttggcgag ggaaagcggc tgactgtcaa atgccgtctg atgagcaccg
    23641 acggtttgtt caaaggtctg gaaggtgtcg gtcagatcga ccgtttgcgc gcttacattg
    23701 gcatattgcc gttgcagatt gcggatgtgc agccttgccc gctcaagctg gccggggtgt
    23761 tggcgtatcg gcatatccag aagcagcgtg gggcggccgg tgcgggcggc gagtgcggag
    23821 acgacggcgg aatcgatgcc gccggaaacg cccacgacaa acccttttgc atttgcccgg
    23881 gcggcgtatt cgtccaacca tcgggcgata tgtgtgataa ctgcctgcgt atccattttt
    23941 cctcctgtcg aaaatatccg catcatagcc tgccggcggt gcaatttgaa tagattgcag
    24001 gcaggttgat gaatattttt cccattcccc gcttaaaaaa gtcatacggg aaaaggcggg
    24061 cggcaagatg gcgcgtgtta taatattgtt gacaatttag gcggcccggt gccgccttag
    24121 gcttcaaaac atattcatat tttttactat ttgattgatt cgatgtctga tttttttcat
    24181 tctgacgtgt tgtccgtttc cgagttgaac gcatttgcca aaagtctttt ggaaaaccac
    24241 cttgccggtt tgtggattgc cggcgaagta tccaacctga cccgtgccgc cagcgggcat
    24301 tattatttct cgctcaaaga cagccgcgca caggtgcgtt gcgcgatgtt caagggtgcg
    24361 gcggtgcgtt tggcgaagcc tttgaaagaa ggcgaccata tcgaggtatc aggaaaaatc
    24421 agtatttatg aagcgcgggg cgaatttcag attaccgtaa acgaagtacg gctcaaaggt
    24481 ttggggcagc tttacgaagc ctatgagcgg ttgaaggcgc agttgcaggc ggaaggcgcg
    24541 tttgcggcgg aacgcaagaa acctttgccc gtccgtccgc aatgtatcgg catcgtaacc
    24601 agtctggcgg cggcggcttt gcgcgatgtc gttactacct tgaaacgccg cgcacccgaa
    24661 atccccgtta tcgtttatcc gacacccgtt caaggcgcag gcagcgaatt gcaaattgcc
    24721 caagcgatta aaaccgcctc gcaacgcgcc gaatgcgatg tgttgattgt ctgtcgcggc
    24781 ggcggcagca ttgaagactt gtgggcgttt aacgaagaac cggtcgtgcg cgccatcgaa
    24841 tcctgcacgg ttccggtcgt cagcggcgta ggccacgaaa ccgatttcac gctcgccgat
    24901 ttcgtcgccg atatgcgcgc gcccacgccg accggtgcgg cggaactggt cagccccaac
    24961 cgccaagaat cgctgcaccg cctcgcccaa gcccaatgcc gtctgaaaac cgttttggaa
    25021 caacgctatt tcgatgccag ccaaaaactc gactggctgg cgcggcaaat ccgtcatccg
    25081 cgccaaaaac tcgacgaaca gcgcgcttca atcggcaagc tggcgcaaac gctgtcttac
    25141 tcgatgacgc aaaacgtccg tacccacacc gcccgtttcg aacgccaaac ccaagccctg
    25201 aaacattgcc gtcctgatgt ttccgtttgc aaaaaaaaca tcgaccgctt tcagacggcc
    25261 ctgtcgcacg ccttccacca actgcttacc caccgccgcc aaagcctgac tgcccaagcc
    25321 gcattgctcg aagccgtttc gccgcagcac attctggagc gcggcttttc cgtcgtcaaa
    25381 aacacacgcg gacaagtcat ccgcaacgcc gatgttttga agcaggggca gaagctgcac
    25441 attatcttcg ccgacggcga aaccgacgtg cgcgtaagca aagaacaagg gcagcaggat
    25501 ttgttcgatt gcatttaaac ccaatcggaa aaaccgctcc cccgccgccg tcattcccgc
    25561 gaaagcggga atccagtccc ttgaccttaa gataatcttt aattcgtcat tcctgaaaaa
    25621 gcgggaatcc ggaagcgaaa agccgcagga atttgtcgga aaaaacagaa acctttccgt
    25681 acacccgaaa tgtttcagac ggcgcatctt ggggcggata aaaacccgac ccgatatttt
    25741 tttataattt tcaataagtt tccattgggc aaatgaaatt ttatttttca actgacagaa
    25801 agaggatgtt atgaagtttt tagaccgtga ggcaagcatt gccaagccgg gtttcaaccg
    25861 ttggctcgta ccgcccgcag cattggcggt acaccttgcc atcgggcaga tttacgccta
    25921 ttccgtattc aacgcgccgc tgaccaaact tatcggcata accgaatcgg cggccggaga
    25981 ttggaagctg acgaccgtag gttggatttt cagtatcgca ctcgcgatgc tgggcgcgtc
    26041 ggccgccctg ttcggtacgt ggatggaacg ggtagggccg cgcaaagcca tatttgccgc
    26101 cgcctgctgc ttcagcctgg gcttcttcgt atccgcattc ggcgtgcgta cgcacaacct
    26161 cttcctgctc tatttgggca acggcgtcat cggcggcgta ggcttgggtt tgggctacat
    26221 cgggccggtt tccacactga tgaaatggtt tcccgacaag ccgggtatgg cgaccggttt
    26281 ggcgattatg ggtttcggtg gcggcgcaat gctggcctcg ccgctttccg tatcactgat
    26341 gaacaccttt tcaaacgctg cttcggtcgg ggttgccgaa accttcgccg tattgggtct
    26401 gctttacctc gcactgatga tgttcggcgc gtttaccatc cgcgtacctg ccgacggctg
    26461 gaaacctgaa ggctataccg cgccgaaaac gcaaaacaag ctggtcagca gcaatcatgt
    26521 caacgtgtcc caagccatga aaacgccgca gttttggctg ttgttctggg tattgtgcct
    26581 gaacgtaact gccggcatcg gcgtattggg tcaggcatcc gtgatgattc aggaactctt
    26641 ttccgaaacc tccataggca ggcaggcagc agtcggcgca ggtgcggcgg caggcttcgt
    26701 cagcctcctg agcctgttta acatgggcgg acgctttttg tggtcgagcg tttccgacaa
    26761 aatcggacgc aaaaacacct acaccatctt cttcgtactc ggttcgctgc tgtatttcgc
    26821 cgttccctcc atcggcgagg gcggcagcaa agccctgttc atcatcggct tttgcgtgat
    26881 tatttccatg tacggcggcg gatttgccgc cattcccgcc tacctgaaag acctgttcgg
    26941 cacctatcag gtcggcgcga ttcacggacg catcctgctg gcctggtcaa ccgccgccgt
    27001 catcggcccg gtactggtca attacatacg ccaaagccaa atcgacagcg gcataccggc
    27061 ggcacaggca tacagcgtaa ccatgtacat catggcgtgc ctgctgatta tcggtttgtt
    27121 gtgcaacctc gccgtcaaat ccgtacacga aaaacaccac gaaaaagaca tcaaaaccgc
    27181 cgcacacagc ggcaaccccg atgacgaaac cgccatatcc gacgcttacc ttgtgggcga
    27241 aaaagtttcc ggcggcggca tatcggtctg gtggcgttgg gcattggccg tcataccgct
    27301 cgcctacggc gtggtgatgg tctttgtcaa agcactcgac cttttctcct gaaaacgccc
    27361 gaataaaatg ccgtctgaaa cagcagatgt ttcagacggc atttttgtgt tttggcgggt
    27421 ttaggctttt ttgcggcggc gttcgcccgg cagcagcatg tccgcccatt tgatgatgtt
    27481 ggcgacttcg gcggggttga gttcgtagaa ctgcccgcgt ttgaggcggt tgggcagtcc
    27541 gatgggaccg aagccgatgc gcacgaggcg gctgacggtg agtccttggc tttcaaaaat
    27601 gcggcgcact tcgcggttgc ggccttcttt aatcacgacg ttgtaccatt tgttcgcgcc
    27661 ttcgccgcct tgttcgcgga tgcgttcgac ttttgccaag ccgtcttcga gcatcacgcc
    27721 ttcttcggtg aggacgcgca tttgttcgcc ggtcagcccg cccaagacgc gcacggcgta
    27781 ttcgcgttcg acttcgaagc tggggtgggc gaaacgttgg acgagttcgc cggaggtggt
    27841 aagaatcaga agtccgctgg tgttgatgtc caagcgtccg atggcgaccc agcggctgct
    27901 ggcggcctgc ggcaggcggt cgaatatgct gacgcggcct tgcgggtcgt cacgggaaac
    27961 gatttcgcct tcttgtttgt aatacaggat gatgcgcggc aggcggtccg cccatttgag
    28021 cttgatgatg ctgcctttga cggtaacgtg gtcgtcgggg gtaactttgt cgcccagttg
    28081 cgcggttttg ccgttgaccg ttatccagcc gttggtaatc cattcttcca tttcgcggcg
    28141 cgagccgacg ccggacgcgg caagcacttt ttgcaggcgt tcgggttcca tgcgcgacag
    28201 gtcgctgcgg cgttctttca aatcgcgcgc gtgttccata attttttggt tgggattgcg
    28261 gacgacgagt tttttggctt tggcggcgcg ctgtttgggg acgttttgag gtttgatgcc
    28321 gtctgaagct ttttgtccgt aaggttggga agcggttttg cccgtttcat ctttgggacg
    28381 ggctttgctt ttgaacggtt tggcggtttt cttggcagac ggggctgcgc cgtcgcgcca
    28441 ttggcgtttg ctggtgggct gtttggacat catttttcct aactcgcctg ataaggcggt
    28501 tcttctgcgg cgtgtgcggc aggacgggtt gataaaatgc cgtcctcggt cggacggttc
    28561 gatattgtac ctgatgcggc ggttttgcgg atgaaatcgt cgctgcggct tggggtcggg
    28621 gcaaagtttg tgcgacggtt gaaatcggtt tgcgtgcggg agtagaatct ttcaaatgat
    28681 aagaggagca gtaaatggat ttgcacaata ttcgggaaga ttacagcaaa cgggaattgt
    28741 cggaagccga ttgcgccgat aatccgatcg agcagttcga gcggtggttg gacgaggcgg
    28801 tacgcgcaca ggtcaacgag ccgacggcgg tcaatgtggc ggcggttgac ggacgcggca
    28861 ggcccaacag ccgtatggtg ctgctgaagg aagttaattc tgaaggtttt gttttcttta
    28921 cgaattatca cagccgcaag gggcgttcgc tggatgctca tccatttgcg gcgatgacgt
    28981 ttttttggcc ggagctggag cgtcaggtgc gcgtggaagg gcgtgttgaa aggctggcgg
    29041 agaagttgtc ggacgaatat tttgagagtc gcccgtatca gagccgtttg ggtgcgtggg
    29101 caagtgcgca gagcgaggtt attccgaaca aggcggtatt ggtggcaaag gcggcggcgg
    29161 ttggactcaa acatcctctg catgtgccgc gtccgccgca ttggggcggc tatattgtga
    29221 ttcccgattt gcttgagttc tggcagggca gaccgagcag gctgcacgac cgcatccaat
    29281 accgtttgct ggatggtggc tggatacgcg agcggctgtc gccttgaaca ggaaaaaaca
    29341 tatgccgtct gaaaaccgga atcgggtttc agacggcatt ttttcacggg gaaaacacgg
    29401 acgggaagtt ttttgtgttc tgaacggaaa gctcgaatcc tctttatacc ggggaattat
    29461 gatgcggacg gcgtgaatgc gtcggagaaa aacaagtttt ccggcagctt atgctgttgc
    29521 acaaacagat tctttgtttg ttcggtcatg gccggagaac cgcaggcaaa tacttcgtat
    29581 tccgacaggt cggggtggtc ttgtgccgcg atgtcttgta cgtgaccatt tcttccctgc
    29641 cagccctctc cggggcggga caatacgggg gtgaagcagg cgtttttcag acggcatgcc
    29701 aacccttgtg cttcttcgag ggcatacaaa tcatcctgat gacgcgcgcc ccagtagaaa
    29761 tggacggcgc ggttgctgcc ttggcggata aggtcgagca ggatgctgcg gatgggggcg
    29821 tagcctgtgc cggttgccag caggatgacg ggtttgccgc tgtcttcctg caaggtaaac
    29881 gaaccgagcg ggcctttaac gcggacgatg cctttttctt tgactttggg ttcgctgccg
    29941 aaaatcattt ccgagcagac accgttttcg tgcctgcgga tgtgcagttc caaaatgcct
    30001 tcttggtcgg gtaaattggc gatggagtag ctgcggctga cgttgcccgg cagcagtaaa
    30061 tcaatgtatt gcccggcgta gaaggcaaac ggcggggctt tgggcagggc aagtttcagg
    30121 agggcgacat cgtgtttgaa aataatactt tcgatgcgtg cgggcagggt gcggacgggt
    30181 agggcatcgg ctttgtagcc ggggatgttg atgttgatat cgctttgcgc agtggtgcag
    30241 cacatcaaaa tcttgccttg cgctttttct gcttcggata aagcctgttc cgagtgtccg
    30301 cccatttgaa tatcgccgct gaccagttcg gctttgcatt gtccgcagac accgcttttg
    30361 caggaatggg gcaggttgag gttttgacgg gcggcagcgg tcaaaacggt ttcgccgtcg
    30421 ttggcggcaa aggtggtttg gtcgggcagg gtaacggtgt ggttcatgtc ttggtatggt
    30481 ttgagcagat ta
//
LOCUS       DAWGRZ010000005        30119 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000005 DAWGRZ010000000
VERSION     DAWGRZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30119
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.005"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(65..904)
                     /locus_tag="WHT44_000151"
     CDS             complement(65..904)
                     /locus_tag="WHT44_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ2532394.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1189..1264
                     /locus_tag="WHT44_000152"
     tRNA            1189..1264
                     /locus_tag="WHT44_000152"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Arg,seq:ccg)
     gene            complement(1329..2522)
                     /locus_tag="WHT44_000153"
     CDS             complement(1329..2522)
                     /locus_tag="WHT44_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ2532395.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSERQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     NRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2543..3631)
                     /gene="trmA"
                     /locus_tag="WHT44_000154"
     CDS             complement(2543..3631)
                     /gene="trmA"
                     /locus_tag="WHT44_000154"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ2532396.1"
                     /translation="MNDYTQQLQGKKDYLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASMIRCDRFEAASEAVNRLMPELIAAAAQSPELKKRW
                     YAVEFLSTLSGEMLVTMIYHKRLDAEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACRTAEGLGSDLLELYCGNGNFTL
                     PLSRYFRQVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLAETH
                     AVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            3778..4878
                     /gene="aroC"
                     /locus_tag="WHT44_000155"
     CDS             3778..4878
                     /gene="aroC"
                     /locus_tag="WHT44_000155"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ2532397.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDIRVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRSAPIAEA
                     MLALVLIDHALRHRAQNADVQVNTPDITLSNK"
     gene            4970..5395
                     /locus_tag="WHT44_000156"
     CDS             4970..5395
                     /locus_tag="WHT44_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ2532398.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDAALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETLSVEAEAAESSAAE"
     gene            complement(5469..7454)
                     /gene="parE"
                     /locus_tag="WHT44_000157"
     CDS             complement(5469..7454)
                     /gene="parE"
                     /locus_tag="WHT44_000157"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ2532399.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     ASDEALGGFATAIDVQIHEDGSLSVHDNGRGIPVGLHPEEGVSVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDEHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDSILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7521..8045)
                     /locus_tag="WHT44_000158"
     CDS             complement(7521..8045)
                     /locus_tag="WHT44_000158"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ2532400.1"
                     /translation="MLDREGYRPNVGIILINNRNEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIIGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQSSDNR"
     gene            8211..9506
                     /gene="serS"
                     /locus_tag="WHT44_000159"
     CDS             8211..9506
                     /gene="serS"
                     /locus_tag="WHT44_000159"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ2532401.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDTLEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMNQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVADSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRLKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9584..10582)
                     /locus_tag="WHT44_000160"
     CDS             complement(9584..10582)
                     /locus_tag="WHT44_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ2532402.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEAEKIGGKYVDLDELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10707..11783)
                     /gene="prfA"
                     /locus_tag="WHT44_000161"
     CDS             complement(10707..11783)
                     /gene="prfA"
                     /locus_tag="WHT44_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ2532403.1"
                     /translation="MKPSILEKLQQLSDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGELDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLEEITNALIAEHQAELLAAMGD"
     gene            complement(11888..12658)
                     /locus_tag="WHT44_000162"
     CDS             complement(11888..12658)
                     /locus_tag="WHT44_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ2532404.1"
                     /translation="MNFTDTHCHLADPALRENLPHILTAARKVGVKRFIVPATRPQDW
                     QDVADLAEMPSEHGQIRIALGIHPWFSDGIAERDFVRLETMLAHYPQAWVGEIGLDFY
                     DKTQTSQQRERQIQVFVRQLEIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANIPGIAKIAAEIRGTCVEEIAKVTERNADALLGR"
     gene            complement(12666..13658)
                     /locus_tag="WHT44_000163"
     CDS             complement(12666..13658)
                     /locus_tag="WHT44_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ2532405.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVSQALSPFSDGLDFE
                     WHVCNPLIDSSSVTLQHWRDWLDIIADKLPSCDGILILHGTDSMAYTANLLALALQGL
                     GKPIVLTGSQWPYAAENSDAPRNLSTAVAAFSLKLKQTVIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGTLAEWDETRGWHNLRLPSQDAAAVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTPADEGFIRAVRDFARQGKLLLNISQVRQGK
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEDVYKELVRLKLV"
     gene            complement(13940..14623)
                     /locus_tag="WHT44_000164"
     CDS             complement(13940..14623)
                     /locus_tag="WHT44_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ2532406.1"
                     /translation="MFAFLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILRFKPIARIMTPKRYEQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFIIMDGLAALISVPIWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFVILGIGATVVAWIWWKKRQRIQFYRSKLKEKRAQRKAAKA
                     AKKAAQSKQ"
     gene            complement(14814..16148)
                     /gene="glmM"
                     /locus_tag="WHT44_000165"
     CDS             complement(14814..16148)
                     /gene="glmM"
                     /locus_tag="WHT44_000165"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ2532407.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEVELEIEAKIDGEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVVDTANGAGYGVAPKVFHELGAQVVSIGNEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGQVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIQKGQQWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWAREGAERIAAAIGGI"
     gene            complement(16278..17135)
                     /gene="folP"
                     /locus_tag="WHT44_000166"
     CDS             complement(16278..17135)
                     /gene="folP"
                     /locus_tag="WHT44_000166"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ2532408.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGVYSQNAQTA
                     LAHAEQLLKEGADILDIGGESTRSGADYVSPEEEWARVEPVLAEVAGWGVPISLDTRR
                     TVIMEKALALGGIDIINDVAALNDEGAVELLARQADTGICLMHMQGLPKTMQINPKYQ
                     DVVGEVARYLKARSAECIAAGIAPQRIILDPGFGSGFGKPLQHNIALMRHLPELMAET
                     GFPLLIGVSRKSTIGELTGEANAAERVHGSVAAALASVARGAQIVRVHDVKATADALK
                     VWEALGINL"
     gene            complement(17275..18168)
                     /locus_tag="WHT44_000167"
     CDS             complement(17275..18168)
                     /locus_tag="WHT44_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ2532409.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHDAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGNLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLNHTKGHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            18259..20373
                     /locus_tag="WHT44_000168"
     CDS             18259..20373
                     /locus_tag="WHT44_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ2532410.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSNGIGPPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPALALR
                     NNSIKIETVNGAFTAGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKGHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(20437..21915)
                     /gene="ubiD"
                     /locus_tag="WHT44_000169"
     CDS             complement(20437..21915)
                     /gene="ubiD"
                     /locus_tag="WHT44_000169"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ2532411.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPISPYLEMTEIADRVLRAEG
                     PALLFENPIKPDGTRYGYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDYFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(21935..22450)
                     /locus_tag="WHT44_000170"
     CDS             complement(21935..22450)
                     /locus_tag="WHT44_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ2532412.1"
                     /translation="MYTCTSCGENHEEMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSRFYDNLENAVYF
                     GMICNQLPPYETDTFGLHCNVVTQPDGQRPLLQLHQGGEHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22457..22936)
                     /locus_tag="WHT44_000171"
     CDS             complement(22457..22936)
                     /locus_tag="WHT44_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532413.1"
                     /translation="MYGREAGWFVWLAAAMCGLWLLRALTAKLPQQRYAALFITLFFA
                     AVLVFGRHDSMYWYPVLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEKAVRYTRR
                     VTQVWCVFFIINGTLAALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYRKLVLKV"
     gene            complement(23171..23416)
                     /locus_tag="WHT44_000172"
     CDS             complement(23171..23416)
                     /locus_tag="WHT44_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ2532414.1"
                     /translation="MTEQEIYRLLRDTLTELFEIEPERITPDTNLYEDLEIDSIDAID
                     LIDRIKRETGRKLQAEDFRNVRTVNDVVQAVLKIQAG"
     gene            complement(23428..23688)
                     /locus_tag="WHT44_000173"
     CDS             complement(23428..23688)
                     /locus_tag="WHT44_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ2532415.1"
                     /translation="MNDLENQIKQLIIDSLALEDITAADIGSEDALFGDGGLGLDSVD
                     ALELGLAVQKHFGFRLDGEQENLREHFANVKTLAAFVKSRQA"
     gene            complement(23685..24443)
                     /locus_tag="WHT44_000174"
     CDS             complement(23685..24443)
                     /locus_tag="WHT44_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ2532416.1"
                     /translation="MDKLDYCRRFFATWLGFVIFGVGGIMMKLVLLPYTLNGTSGSVA
                     RQLAARRIIGTSWRLFVAYLKWSGVLEVSFKGVEKLNRPGQLILANHPSLLDVVLLVG
                     HVPEMNCIVKKDLQHNPAMSSQIKGAGYIPNEESEAMLETVKAVFDSGQSLLVFPEGT
                     RTGWDGRVKMHRGAVSLGLRYAEVITPVCIKMNPPNFKKGQPWYRIPPKRIRYEITVG
                     DDILPQDWLAEKPLPIAARRLNEYLQDYFTRKTI"
     gene            complement(24436..25191)
                     /locus_tag="WHT44_000175"
     CDS             complement(24436..25191)
                     /locus_tag="WHT44_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEZ2532417.1"
                     /translation="MPSIICRFHFDIAAWRVSGSKMRDMAQWAKWAECPDFADGLPDV
                     RPELPFLPAMQRRRLSKAARLVCDAAWDIASAHPGSPVVYASHDGEMARSFDLWLELL
                     KSHTVSPTSFGLSVHNATAGQWSILRQDMSEQTALAVCADGVETALAEAASLLEEGCG
                     SVLVLAADDPLPEGYAVSATRAPFAYALAMVLTKGTRYSLTLSASDDMPSEAGMLPEA
                     YWSGLEWVRFLLNGSRECRRVYRNREWLWQRNG"
     gene            25346..25879
                     /locus_tag="WHT44_000176"
     CDS             25346..25879
                     /locus_tag="WHT44_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester dehydrase"
                     /protein_id="HEZ2532418.1"
                     /translation="MPVFASLPPQYMDTLPECPITNTASLLPHSGRMVLIDRITRYGD
                     DFVEAGAQVSPNHILLLDDKLPYTAFIELMAQAVGAYAGIQARKNARSVRLGFLLGTR
                     KLEIFAQSVPIGTHLLATAHMSIQDAGGMGVFDCELRWTDAPETSSETLPSDGILARA
                     SLNVYSPEHPAGTTDAV"
     gene            25907..26635
                     /gene="fabG"
                     /locus_tag="WHT44_000177"
     CDS             25907..26635
                     /gene="fabG"
                     /locus_tag="WHT44_000177"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ2532419.1"
                     /translation="MTETVLITGSNRGIGKAVAFGLAEDGFDIAVHCRSRRDEAEAVA
                     EEIRALGRNARVLQFDVSDREACREILTADIEANGAYYGVVLNAGLTRDNTFPAFSDD
                     DWDVVLRTNLDGFYNVLHPLVMPMIRRRKAGRIVCMASVSGLTGNRGQVNYSASKAGI
                     IGAAKALAVELAKRKITVNCVAPGLIDTDIIDENVPVEEILKAVPAALMGLPEEVAHA
                     VRFLMDEKAAYITRQVIAVNGGLC"
     gene            26629..27879
                     /locus_tag="WHT44_000178"
     CDS             26629..27879
                     /locus_tag="WHT44_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEZ2532420.1"
                     /translation="MLNTRRVAVTGIGGITAFGRDWQSIQAAFKAEKNAVKYMDWHER
                     FPELEAQLGAPIENYAPPKHWTRKQLRSMGRVSYLCVDAAEQALADAGLLGDESITDG
                     RMGVACGSSSGSTKDIGDVGELLLTGTSRNFSANTYVRMMPHTTAANIGIFFGLKGRI
                     IPTSSACSSGSQGIGYAYEAIKYGLTDMMLAGGGEEFFPSEVYVFDSLYAASRRNGEP
                     EKTPRPYDANRDGLVIGEGAGIFVLEELEHAKRRGAIIYAELVGYGANSDAYHISTPR
                     PDAQGAILAFQTALQHANLAPEDIGWINLHGTGTHHNDNMESRAVAAVFGNNTPCTST
                     KPQTGHTLGAADAIEAAFAWGIADRQSNPEGKLPPRLWDGQNDPNLPAINLTGSGSRW
                     ETEKRITASSSFAFGGSNCVLIIG"
     gene            27933..28008
                     /locus_tag="WHT44_000179"
     tRNA            27933..28008
                     /locus_tag="WHT44_000179"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27966..27968,aa:Ile,seq:cat)
     gene            28083..28841
                     /locus_tag="WHT44_000180"
     CDS             28083..28841
                     /locus_tag="WHT44_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ2532421.1"
                     /translation="MKKVSVLIVAKNEANHIQECIESCRFDKEVIVIDDYSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            28842..29906
                     /locus_tag="WHT44_000181"
     CDS             28842..29906
                     /locus_tag="WHT44_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ2532422.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLYALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIVEHHQSGILLDRLTPESLADEIERLVLNPETKNALATAGH
                     QCVANRFTINHTADKLLDAI"
BASE COUNT         6963 a         8485 c         7822 g         6849 t
ORIGIN      
        1 tcccataaaa aaacggagca gatacctgcc ccgtttttat ttaatccgaa attttaatct
       61 aaatttagaa ttttgcaccg gattggtttg ccatatagtc aacagccgct ttgacttcgt
      121 catcgctcaa accggcattg ccgcctttgg ccggcatcgc gttaaagcct tcaagggcgt
      181 gtttgtgcaa ggtttctttg ccttttttga tacgcggtgc ccaatcgtct tttttgccta
      241 tgccgggaat accgggaatc gaaccgccgt ggcacacctg acaggttgct tcgaagactt
      301 ttttaccgtc aacgccgacc gcaggggctg ccgcaccctt gtcttctgcc ttcgcttctg
      361 ccggagctgc actatcggca ggagcagaag ctgttcctga agcggcattg tcggcaggcg
      421 cagcctcatc aggattcggg aaagaaccgc cgcttttgtt cgccatgtaa gtaatcgccc
      481 gtttgagttc ctgatcggtc aggtctgccg caccgccttt ggcaggcatg gcgttaaagc
      541 cgttcagcgc gtgttggaac aaggtatcga agccttgcgc gatacgcggt gcccaatcgc
      601 cgttgtgttc cagtttcgga gcgttcggca cattgctgtc cgccgcgtgg cattggatac
      661 agattttgcc gaaaatctgt tcgccttggc gttcgccgac ggggatgccg tcgcccatcg
      721 tcaattgtcc gacaggctgg atacgggtct gcgttgctgc ttccgtagtg gcatcgacat
      781 cgccgaacga gccgctgccc gccagcttaa tcaggaaata aaggactgca ataacaataa
      841 cgataccgct cacaagggta aacagtgcag agccttgggc tttgttgtcg cggagttgtt
      901 tcatttggta ggcctcgccg tcaggttagg ttgtgctgta aattatagtt tggtgtgtta
      961 aacgcagtta acaatatttt gctggattat actgaattca cagggtcttt ccaatcgcta
     1021 tcattgaaaa tatgaaaaaa tttgccaacg gtatctgtat aaaacaaaca atcctttgaa
     1081 aataatggtt tatcctcaag aaaactctcc ttatgccgcc atacgccgcc tgccggcgca
     1141 agataacctt tgccaatttg cagaatttac gttaaccttg cgttttccgc acccatagct
     1201 cagttggaag agtgtcagtt tccgaagctg gaggtcacag gttcgatccc tgttgggtgc
     1261 gccaattata aagagaccgt ctgaaagata aatatttttc agacggtctt ttgacttact
     1321 tcaaactctt atttcaagac ttccgcaaat gcgcgggcaa catagtcggt attcgacgta
     1381 ttcagtccgg cgacgcacat cctgccggaa tccagcaggt aaacggcaaa ttcgtcgcgc
     1441 agcctgcgga cttgttccac gctcaatcct gtgtagccga acatgccgcg ctgtttgatg
     1501 aaataagtga aatcgcgatt ggggatttgc gcagttaaga catcataaag tttctgccgc
     1561 atcgcacgga tgcggtcgcg catcatataa acctcgtttt gccacaaggc gtaaagttcg
     1621 gggctgttca tcacgtcggc ggcgatatac gcgccgtgcg cgggcgggct ggagtagatg
     1681 cggcggacgg tgaatttgag ctgtccgaac accaaatccg cttcttcctt attcgggcaa
     1741 accacgctta agccgccgac gcgctcgccg tagagcgaca ggttttttga gaaggaattg
     1801 ctgacgaaca agggcaattc catttccacc gctttgcgga cggcgtaggc atcgctgtcc
     1861 aaatcgccgc cgaatccttg gtaggcaatg tccataaacg gaatcagttt gcgcgttttg
     1921 atgatgtgca acacttcgtc ccattgccgt tccgacatat ccacgccggt cgggttgtgg
     1981 cagcagggat ggaggatcag gacgctgttt tcgggcaggg tgttgaaaaa cgcggtcatt
     2041 tcgtcgaatt tcacgccgac agtggcaggg tcgtaatatg ggtaagtgcc gacctcgaaa
     2101 cctgcgcctt caaaaatgcc gcgatggttg tcccaagtcg ggtcgctgac gtaggcgcgc
     2161 gcttcgggaa accagcggtg caggaagtcc gccccgactt tgagcgcgcc cgagccgccc
     2221 aaggtttgta cggtaacgat gcgcccttgc gcaagcgcgg gattgtcttt gccgaacaat
     2281 aaatgctgca ccgcgctgcg gtaagtgtcc aagccttcca tcggcaggta gggcgacggc
     2341 gcaggcgcgg cggcacgggc ggtttcggct cggctcacgg attccaacac gggcattttg
     2401 ccttcgtcgt cgaaataaat gcctatgctc aaattgactt tttcggggcg cgggtcgttt
     2461 ttgaaggttt cgaccaaact caaaatcggg tcgccaggat agtattcgat gtgtcggtac
     2521 atagtcctta cctcttgctt tttcaaagga ttttcttttt caacaataca ccactttcga
     2581 tatggtgcgt aaacgggaat tggtcgaaca gggcggcacg ttcgaccgca tgggtttccg
     2641 ccaaggtgtc caaattggcg cgcaacgttt cgggattgca ggaaatgtag atgatgttgt
     2701 caaactgcga caccagcttc aaagtttcct catcgatacc ggcacgcggc ggatcgacga
     2761 aaatagtgga aaatgcgtaa tccgtcaaag caataccgcc atccttaagg cgtttaaact
     2821 cacgttttcc ggtataggct tcggtaaatt cttcagcaga cagacgggcg attttgatgt
     2881 tgccgatgcg gttggcttcg atattccatt gcgccgcgct gacggaggtt ttggagattt
     2941 cggttgccaa aacctgtcgg aaatatcggg acagcggcag ggtgaaattg ccgtttccgc
     3001 aatacagttc gagcaggtcg ctgcccaagc cttccgccgt gcggcacgcc cattcaagca
     3061 ttttctgaca cacggcggca ttcggttggg taaaactgcc ttcaatttgc cgataacgga
     3121 aatcccggtt gccgaccttc aaagtttccg ttacatagtc ctgttttaag actattttct
     3181 gtcccctgct ccgcccaata acggaaatat ccaactgttg ctgtaacgct tgcgccgcct
     3241 gcatccactc agcatcaagc cttttgtggt aaatcatggt aaccagcatt tccccgctga
     3301 gcgtggacag aaattcgacg gcataccagc gttttttgag ttcgggggat tgcgcggcgg
     3361 cggcgatcag ctcgggcatg aggcggttga cagcctcgga agctgcttca aaacggtcgc
     3421 agcgtatcat gcttgcgccg ctggctttct gccctttttc aaacatggca taaaacattt
     3481 ccccgccttc gtgccaaata cggaactcgg cacgcatacg gtaatgtttg tccggagatt
     3541 cgtacacttc ccactcagga acatccaaac ctgcaaaaag ggttttaagg tagtcttttt
     3601 taccttgaag ctgctgcgtg tagtcattca tatcgctatc ctgaaaaatc agaccggatt
     3661 ataagccgat ttgcctcgcc gcacgcaaac tctttgactg ccgccccttc aatgacggac
     3721 gggcttttgt gctaaaatcc gccatctttc cacactatac cgataaaggg aaaaatcatg
     3781 gcaggcaaca ctttcggaca actcttcacc gttaccacct tcggcgaaag ccacggcgcg
     3841 ggtttgggct gtatcatcga cggctgcccg cccggcttgg aattaagcga agcggatatc
     3901 caatttgacc tcgaccgacg caaacccggc accagccgcc acgttaccca acgccgcgaa
     3961 gccgaccaag tcgaaatcct ctccggcgta ttcgaaggca aaaccaccgg cacgcccatc
     4021 gccctcttaa tccgcaatac cgaccagcgc agcaaagact acggcaacat cgccaccagc
     4081 ttccgccccg gccacgccga ctatacctat tggcacaaat acggcacgcg cgactaccgc
     4141 ggcggcggca ggagctccgc ccgtgaaacc gccgcccgcg ttgctgccgg agccgttgcc
     4201 aaaaaatggt tgaaagaaaa attcggcacg gaaatcaccg cctacgttac ccaagtcggc
     4261 gaaaaagaaa tccggtttga aggctgcgaa cacatttccc aaaatccttt ttttgccgcc
     4321 aaccatagcc aaattgccga gctggaaaac tatatggaca gcgtgcgcaa atccttggat
     4381 tccgtcggcg cgaagctgca tatcgaagca gccaatgtcc ctgtcggctt gggcgaacct
     4441 gtttttgacc gcctcgatgc cgaaatcgcc tacgcgatga tgggcatcaa cgccgtcaaa
     4501 ggcgtggaaa tcggcgcagg ttttgacagc gtaacgcaac gcggcagcga acacggcgac
     4561 gaactgaccc cgcaaggctt cctgtccaac cactcaggcg gcatcctcgg cggcatcagc
     4621 accgggcaag acatccgcgt caatatcgcc atcaaaccca ccagctccat cgccaccccg
     4681 cgccgcagta tcgacatcaa cggcaacccc atcgaactcg ccacgcacgg caggcacgac
     4741 ccctgcgtcg gactgcgctc cgcgccgatc gccgaagcca tgctcgcgtt agtcctcatc
     4801 gaccacgccc tgcgccatcg cgcgcaaaat gccgacgttc aggttaatac gcccgacatt
     4861 accctttcaa acaaataaaa atttagccaa aacacagact ttataataga atatcgagca
     4921 ttgccgtgca gccttgacgc acgggtttgt ttagaggaat acaaccgaaa tgacacaaga
     4981 aaccgctttg ggcgcggcac taaaatccgc cgtccaaact atgagcaaaa agaaacagac
     5041 cgaaatgatc gccgaccaca tctacggcaa atacgatgta ttcaaacgct tcaaaccgtt
     5101 ggcgctcggc atcgatcagg atttgattgc cgctttgccg caatacgatg ccgcactgat
     5161 tgcacgcgtc ctcgccaacc actgccgccg tccgcgctat ctgaaagcct tggcgcgcgg
     5221 aggcaaacgt ttcgatttga acaaccgttt caaaggcgaa gttacccccg aagaacaggc
     5281 gatcgcgcaa aaccatcctt ttgtgcagca ggctttgcaa cagcagtccg cccaagctgc
     5341 cgccgaaacg ctgtctgttg aagccgaagc agccgaatct tccgcagcag aataaatccc
     5401 caaacgaaat gccgtctgaa aaccgatttg gtttcagacg gcattttttc gtatgcggca
     5461 atcacggttc aaatatccaa ttccgccgta tcgccttcgc gttccatcca agcgcggcgg
     5521 gcggcggctt cgcctttgcc catcagtttg acgaagatgt cgcgcgtctc gtcatctgca
     5581 ccttctggga tttgtacctg caacaggcgg cgggtgtcgg ggtgcatggt agtatctttg
     5641 agctggtcgg ggttcatctc gcccaagcct ttgaaacggc tgatggaata ggcggtttct
     5701 ttaacgcctt ctttttgcag ccgctccaaa atgctgtcga gttcgttttg gtcgagggcg
     5761 tagaatttgc gggcaggttt actcttacct tgtgcgttga catcgacgcg gaacagcggc
     5821 ggctgggcga cgtagatgtg tccgtcggca accagtttcg ggaagtggcg gtagaacagg
     5881 gtcagcagca aaacttgaat atgcgagccg tccacgtcgg catcggacag gatggcgatt
     5941 ttgccgtagc gcaggccgct taaatcggga tggtcgttaa taccgtgcgg atcgacgccg
     6001 atggcgacgg aaatgtcgtg gatttcggcg ttgccgaaga gttggtcggg gtggacttca
     6061 aagctgttga gcactttgcc gcgcaggggc aggatggctt gggtggcttt gtcgcgggcg
     6121 agtttggctg agccgccggc ggaatcgcct tcgacgagga agagttcgtt ttcgcggatg
     6181 tcttcgcttt cgcagtcggt cagcttaccg ggcaggacgg cgacgccgct gccttttttc
     6241 ttttcaatct ttttaaccga acgcatccgc gcctgtgctt ggcggatggc gagttcggcg
     6301 atttttttgc cgaagtccac gttttggttc agccacaatt ccaaagggtc gcccgatacg
     6361 gtggcgacga gtttcagcgc gtcgcggttg gtcagcttgt ctttggtttg accttggaac
     6421 tgcgggtcga ggacgcgggc agagagaacg aaggcggttt ttccgaacac gtcgtcgctt
     6481 tgcactttaa cgccgcgcgg caagaggttg tgcagattga tgaagttgtt gactgcgttg
     6541 aacacggctt gtttcaagcc tgcttcgtgc gtgccgccca gcggggtggg gatgaggttg
     6601 acgtagcttt cgttggcgca cgagccttct tccagccaag tcagggcaaa cgccgctcct
     6661 tcgccgatgc tgaaatcgcc gttgtgttcg tctgaaatgt agttttcgca agagaacagc
     6721 ggtacggctt cctgcgcgtc ggcaatcagg tcggtcagat agcttttcag gccgtcgggg
     6781 taatgccagg tttgggtgtg cgcttcgtct tcgcctttga ccggacgggt cagggaaacg
     6841 cgcacacccg gcagcagcac ggctttggca cgcagcaggc gttcgagttc gggaatgctg
     6901 taattcgggc tttcaaaata tttgccgtcc ggccagacgc gcacttctgt accgctgtct
     6961 ttgacggcgc atttgcccac ttgtgccaac ggttcgacca cgtcgccgcc ggcaaacacg
     7021 atgcggtgga ttttgccttc gcgtttgacc gttacttcaa ggcgggtgga aagggcgttg
     7081 gtgacggata cgcccacgcc gtgcaggccg cctgaaaagg catacgcgct gcctccgtct
     7141 tttttgttga acttgccgcc tgcgtgcaga cgggtgaata cgagttcgac tacggatacg
     7201 ccttcttcgg gatgcaggcc gacgggaatg ccgcgcccat tgtcgtgcac ggaaagcgaa
     7261 ccgtcttcat gaatttgcac gtcgattgca gtcgcgaaac cgcccaacgc ttcatccgac
     7321 gcgttgtcga tgacttcttg gcagatatgg gtcgggctgt cggtgcgggt gtacataccg
     7381 ggacgttctt tgaccggctc caagcctttg aggacggtga tgctggattc gctgtattgg
     7441 ttgtttttag ccatgggaat aatctgaaag taagaaaaac aacgctttca gacggcctga
     7501 aagcgttgcg ttccgttgtt ttagcggttg tcggaagatt ggcgggcggc aaagtcttca
     7561 taactttcca taccgcgcag gaagcgggaa gagagttctt tcaacgcccc caaataaacg
     7621 tcgcgtttga aatcaatcac ttcgtcaacg ggtgcccaat attgatgcca acgccagccg
     7681 tcaaattcgg ggtggcgggt ggcgcgcagg ttgacatcgc aatctcggcc ggtcaggcgc
     7741 aggagatacc aaatctgctt ctgtccgcga taagagccgc gccattcgcg gcgtacccag
     7801 ttgttcggca cgtcataacg cagccagtcg cgcgtgcggc cgataatttt gacgtgttgc
     7861 ggcaaaagcc cgacttcttc gtacaactcg cggtacatgg cggtttcggg gctttcgccc
     7921 ggcttgatgc cgccttgcgg aaactgccaa gaatgttcgc gcacgcgctt accccaaaag
     7981 acttcgttgc ggttgttgat taagatgata ccgacattgg ggcgatagcc ttccctgtcc
     8041 aacacggtgt cgccctccgt taaattcaat cttgggattt tcccacaaat caggcggttt
     8101 tgacaaatca gacggcatgg cggtacgcgt gccgaaacac ggggggattt gggaaaatat
     8161 cttaaatttg gtttacaata atgtatttca aattattcgg gaatcagacc atgttagata
     8221 tccaattgct ccgcagcaac accgccgccg ttgccgaacg gcttgcacgg cgcggttatg
     8281 actttgatac cgcacgtttt gacacactgg aagaacgacg caagtccgtt caggtgaaaa
     8341 ccgaagaatt acaggcctcg cgcaacagca tttccaaaca aatcggcgca ctgaaaggtc
     8401 agggcaaaca cgaagaagcg caggcggcca tgaatcaggt tgcccaaatc aaaaccgatt
     8461 tggaacaggc tgccgccgat ttggatgccg ttcaaaaaga attggacgca tggttgttga
     8521 gcatacctaa cctgccgcac gaaagcgtac ctgccggtaa agacgaaacc gaaaacgtcg
     8581 aagtccgcaa agtcggcacc ccgcgcgaat ttgactttga aatcaaagac catgtcgatt
     8641 tgggcgaacc tttgggtttg gattttgaag gcggtgcaaa actctccggc gcacgattta
     8701 ccgtgatgcg cggacaaatc gcccgtctgc accgcgcctt ggcacagttc atgctggata
     8761 cgcacacgct gcaacacggc tacaccgagc attacacgcc ttatatcgtt gacgatacga
     8821 cgctgcaagg tacgggccaa ctaccaaaat ttgcggaaga tctgttccac gttacccgtg
     8881 gcggcgacga aaccaaaacc acccaatacc tgattccgac agccgaagtt accctgacca
     8941 ataccgttgc cgacagcatt atcccgtccg aacaactgcc gctgaagctg accgcgcatt
     9001 cgccctgttt ccgcagcgag gcgggttcgt acggcaaaga cacgcgcggt ctgattcgcc
     9061 agcaccagtt cgacaaagtg gaaatggttc aaatcgttca tcccgaaaaa tcatacgaaa
     9121 cgctggaaga aatggtcggc catgccgaaa acatcctgaa ggctttggaa ctgccctacc
     9181 gcgtgattac cctgtgtacc ggcgacatgg gcttcggcgc ggcaaaaacg tatgacttgg
     9241 aagtttgggt gccggcgcaa aatacctacc gcgaaatctc aagctgctcc aactgcgaag
     9301 atttccaagc ccgccgcctg aaggcgcgtt tcaaagacga aaacggcaaa aaccgcttgg
     9361 tacatacttt gaacggctcc ggcttggctg tcggcagaac gctggtcgcc gtattggaaa
     9421 accatcaaaa cgccgacggc agcatcaaca tccctgccgc actgcaaccg tatatgggcg
     9481 gtgttgccaa gttggaagtc aaataagttt gcaggctgcc tgaacgtcaa atgccgtctg
     9541 aaacctgttt cagacggcat ttcctttaaa cttttaaaac acgtcagccg tcggcacgaa
     9601 ccgcattgcc gcaatcgccg gtctgtccga cctcgcggat attggacagc gtaacttccg
     9661 aaatattacc caacgcctct tccgtcaaaa atgcctgatg gccggtaaac agcacattat
     9721 gacaagacga caggcggcgg aacacgtcgt cggtaatcac atcgttggat ttgtcttcaa
     9781 aaaacagctc gcgctcgttc tcgtacacat ccatgcccaa tgcgccgatt ttccggcgtt
     9841 tcaacgcctc aatcgcggcg gcactgtcaa tcagcccgcc ccggctggtg ttgataatca
     9901 tcacgccgtc tttcattttg tcgaacgccg cttcgttcag catatagtgg ttttccggcg
     9961 tggcggggca atgcagcgtg atgatgtccg accgggcata cagctcgtct aaatccacat
    10021 atttgccgcc gattttttcc gcttcggggt tgcaaaacgg atcgtaagcc agcaggttca
    10081 tgccgaaacc ctttaaaatc cgcatggttg caataccgat tttccccgtg ccgataacgc
    10141 ccgccgtttt gccgtacata ttgaaaccgg tcagaccttc cagcgaaaaa ttcgcatcgc
    10201 gggtacgctg ataggctttg tggatacggc ggttcaacgt cagcatcaga ccgaccgtat
    10261 gttccgcaac cgattcgggc gaataggcag gcacgcgcac gactttcaag cccaactctt
    10321 cagccgcctt taaatccaca ttattgaagc cggcacaacg caacgccaca gttttcacgc
    10381 caatttgcgc caatttttcc aacacgggcc ggctgccgtc gtcgtttaca aaaatacaga
    10441 ccgcttccgc gccttccgcc attttcgccg ttttcgcatc cagcataaaa tcaaaaaact
    10501 ccagctcgaa gccgaaatgc cggttggcgc gggtaaaatg ttcgcggtca tagcttttcg
    10561 taccgtaaat cgcaatcttc atcaatatgt ccagttgtcg tctatggttg agaaacggca
    10621 taatacactg aattcaaaca aatcagtaga atatggtgga ttaaaattga ttgcatgcac
    10681 ggcatttcca tttcaaaaca caaaactcaa tcgcccattg ccgccagaag ctcggcctga
    10741 tgctcggcaa tcagggcatt ggtgatttct tccaagtcgc cgtccatcac aaaatccagc
    10801 ttgtgcaggg taaggttgat gcggtggtcg gttacgcggc cttggggata gttgtaggtg
    10861 cggatgcgtt cgctgcggtc gccgctgccg atgagggatt tgcgctcggc ggcttctttg
    10921 gcttgggctt cgcgtttttg cgcgtcgttc aggcgggcgg cgaggacttt cattgcctgc
    10981 gctttgttgg catgttggct gcggccgtct tggcattcga ccaccatgcc ggtgggcagg
    11041 tgggtgatgc ggacggcgga gtcggttttg ttgatgtgct gaccgcccgc gccggatgcg
    11101 cggaaggtgt cgatgcgcag gtcggctggg ttcagttcga tgtcttccag ttcgtccgct
    11161 tcgggcatga cggcaacggt gcaggcggag gtgtggatgc ggccttggct ttcggtggcg
    11221 gggacgcgct gcacgcggtg tccgcccgat tcaaatttca aacggctgta cgccccgagt
    11281 ccgacaatac gggcgatgac ttctttatag ccgcccaatt cgctttcgtt ggcggacacg
    11341 atttcaacct gccaacggtt gcgctcggcg tagcggctgt acatacgcag caaatcgccg
    11401 gcaaacagcg cggcttcgtc gccgcccgtt ccggcgcgta tttcgatgaa gatgtttttg
    11461 tcgtcgtcgg catctttggg cagcagcagt ttttgcagtt cggtatcgag ttcgccgatt
    11521 ttggctttgg ccgcttcgat ttcttcggcg gcaaagtctt tcatttcggg gtcggacaac
    11581 atttcttcgg catccgccaa gtcgctttgg gcaagccgat agttttggaa cacttcgacg
    11641 acgggggtca gttcggcgtg ttcgcgcgtg agcttgcggt agttgtccat gtcggacgtg
    11701 gcttcgggct gtccgagaag gtgggtaact tcttccagtc ggtcgctgag ttgttgtagt
    11761 ttttctaaga tagacggctt cataattctt ccataacaaa cgccgcctga atgttcagac
    11821 ggcatcaaca ctggattatt ataataggtt ttccggatat tcaaaaagat aatcttagat
    11881 ggataaccta ccgtcccaac agggcatcgg cattgcgctc cgttaccttt gcaatctctt
    11941 ctacacaggt tccgcggatt tccgcagcaa tctttgcaat acccggaata ttggcaggcg
    12001 tattaatctc ttttttcagc ataaacgggc tatccgtttc caatacgaaa tccccgtcgt
    12061 tcaaggcttt aagcgtatcg cgcactttac gcgcgttcgg attgagcagc agcgaaccga
    12121 tgccgatttt gaaacccagt ttcgtcaaca cacgcgcttc ttccgcgctg ccggagaagg
    12181 cgtgaacgat gccgccttgg gcaaagcctg tctgtttgac ggcggcggcg atgtcggcgg
    12241 tggctttgag attatggata atcacgcggc ggcgcagggt ttgcgcaatt tcaagctggc
    12301 ggacgaaaac ttgaatttgc cgttcgcgct gctgcgacgt ttgggtttta tcgtaaaaat
    12361 ccaagccgat ttcgccgacc catgcctgcg gataatgtgc caacatcgtt tccaggcgga
    12421 cgaaatcccg ctcggcaatg ccgtctgaaa accaaggatg aatgcccagt gcaatacgga
    12481 tttgaccgtg ttcggacggc atttccgcca aatccgccac gtcctgccaa tcctgcgggc
    12541 gcgtcgcggg aacgataaac cgcttcaccc caactttccg cgctgcggtc aggatgtgcg
    12601 gcaggttttc gcgcagggcg ggatcagcga gatggcagtg ggtgtcggtg aagttcattt
    12661 cgatttcaca ctaactttag tcttaccaat tctttgtaaa catcttcctt accccagcct
    12721 tgcgatacgg cgagggtcat cagcgcggtg gcggtttcga ggttgcattt gccgccgttg
    12781 atgatgcccg agttgcggaa cgcgttgcct tgcgcgtaaa cggcggcggt tttgccttgt
    12841 cggacttggc tgatgttgag cagcagtttg ccctgccgcg cgaagtctcg gacggcgcgg
    12901 ataaaacctt cgtctgcggg cgtgttgccg tgtccgtagc tttgcaggat aagagcttgg
    12961 gcgggaagct gtccgagtcc gtccgcaagt tcttggacgg caaagccggg gataagcgtg
    13021 cggacagcga tttttgcctg cgggtcggga taacggattt tgaggccgtc tgaaacggct
    13081 gctgcgtctt gggacgggag gcgaagattg tgccaacccc gggtttcgtc ccattcggca
    13141 agcgtgccga aatgcggatt gtcgaagcct gcagcagttt cggtgctgac tttgctgctg
    13201 ccgacggcgg gatacagttt gccgtcaaac gcgatgacgg tttgtttgag cttgaggctg
    13261 aaggcggcaa cggcggtgga gaggttgcgc ggggcatcgc tgttttcggc ggcgtaaggc
    13321 cattgggaac cagtcaggac aatcggtttg cccaaacctt gcagagcgag cgcgaggaga
    13381 ttggcggtgt acgccatgct gtccgtgccg tgcagtatca ggatgccgtc gcatgaaggg
    13441 agtttgtcgg caatgatgtc cagccaatcg cgccagtgtt gcagcgtaac ggaggaggaa
    13501 tcaatcaagg gattgcagac gtgccactcg aaatcgaggc cgtctgaaaa gggggaaagg
    13561 gcttggctaa ccagtgcggt atcggggcgc aggccttcgc tgctttgggt catgcctatg
    13621 gtgccgcctg tgtagaggac gaagattttt tgtttcatgg acatcatcgg gtcgtctgaa
    13681 aataataata cggcttattt aactatattt cggacagact ggcaatttgg cggcgcggac
    13741 ggttttcaga cggccttcaa atgaaaaagc acccgagggc tgtcgatatt tgattttcca
    13801 agtagatttt tattcacgaa ataggagagc cgcaacaagc ttaaatccct tgtgaggttc
    13861 ccaacacgga agataccgct ttgtggatta aaaaatacgg aaactattga atatcgacaa
    13921 cctatttagg tgcttgattt tattgtttgc tttgcgcggc ttttttggct gccttggcgg
    13981 ctttgcgttg cgcccgcttt tctttcaatt tgctgcggta aaactggata cgttggcgtt
    14041 ttttccacca aatccaagcg acaacggtcg cacctatacc caagataaca aaaatacccg
    14101 attgcaggct gtgcattttc gccatcagcc aatcgatgtt gtgcgcaccg tattcgccca
    14161 gataaatcca aatagggacg gaaatcagtg cggccagtcc atccataatg ataaaacgca
    14221 agtatgaaac cttgcggctg ataccggctg taacaaatac ggccgttctc aagccgggca
    14281 ggaaacgggc gacaaataag acccagttac cgtatttgtc gaatttttcc tgaacctgct
    14341 cataacgttt cggcgtcatg atgcgcgcaa taggtttgaa ccttaggatt ttctgccccc
    14401 aaattcgtcc ggcggcgaac atgatgccgt ccccgaccaa tacgccgagc ataccgactg
    14461 caaacataat atgcggattg gtataaccca tacccgaaat cacgccgcct gttaccaagg
    14521 tcaaatcctc gggaatcggc acgccgaaac cgcagatgac caatacaaaa aaaacagccg
    14581 cataaccgta ttcgacaaaa aaggcttcta aaaaagcaaa catggcggat attccattgt
    14641 cggagataaa aagtcagaac aaaccgaaac attttctaca tgaagcaggc attctatcaa
    14701 agattatgcc gtctgaaagc ggaaaaaagg cagattcgcg catcctccgc atgttcagac
    14761 ggcatttaaa cagaaaaccg gccggttcta aaaacggttt tgcctgattt tgcctaaatg
    14821 ccgccgatgg cggcggcaat gcgttccgcc ccttcgcgcg cccaatccgc ctgccgcgcc
    14881 tccaccatca cgcgcacgac gggttcggtt cccgaagcgc gcaacacgac acgccctttg
    14941 ccttcgagtt ctttttccac ttccgccaac acgtctttcg aagcttcctg ccattgctga
    15001 cctttttgga tgcgcacgtt aatcatcgtt tgcggatacg gctgccaatc ggcgcaaacg
    15061 gtggcgaggt cttggttcag cgtttgcagt gccgccaaaa cttgcagcgc ggaaataatg
    15121 ccgtcgccgg tgttgtgttt gtccatacac aaaatatggc cgctggcttc gccgccgatg
    15181 agccagccgc gttggttcag ctgttccaac acatagcggt cgccgacttt ggcgcggcag
    15241 aaatccacgc cctgctcttt cagggcgatt tccatcgcca tattggtcat gaccgtgccg
    15301 accacgccgc cgatgttgat accttctcgg gcgcgggctt tggcaatgac gtaaatcagg
    15361 ctgtcgccgt cgtaaacctg cccgttttta tcgaccatca tcaggcggtc gccgtcgccg
    15421 tctaaggcga tgccgtagtc ggcttcatgc tgtaaaacgg cggcctggag tgtcttggta
    15481 taagtcgcac cgcatttttc gttgatgttg tagccgttgg gttcgttgcc gatgctgacg
    15541 acctgtgcgc ccagttcgtg aaacaccttg ggggcgacac cgtaccccgc gccgttggcg
    15601 gtatcgacaa ccaacttcaa accccgaagg tcggaatggc tgggaaaggt ggatttgcaa
    15661 aattcgatat agcggtcgtc cgcaccgctg atgcggcgtg cgcgaccgag acgggcggac
    15721 ggttgggttt tcatttcgcc gtcgattttg gcttcgattt ccaactcgac ttcatcggaa
    15781 agtttcacgc cgccttcggc gaagaatttg atgccgttgt cggaataggc gttgtgcgac
    15841 gcggaaatca tcacgccggc ggacaggcgc aacgcgcggg tcagataagc cacgccgggc
    15901 gtgggcagcg gtccggtctg taccacattc acacccgccg ccgtaaaacc ggccaccaaa
    15961 gcggcttcca gcatatagcc ggaaatgcgc gtgtctttgc cgatgaggac ggtcggtttc
    16021 tggtcggtgt cgtgctgcac caaaacctgc cccgccgcat agccgagttt caatacgaaa
    16081 tcgggcgtaa tcggaaattg ccccacttcg ccgcgcacgc cgtccgtgcc gaaatatttt
    16141 tttgccatgt gttgctccga gaatgtgaac cgttgtccga gattatacag tcagtttgtg
    16201 ccttgctgtc tgcaccgttg atgccgtctg aaaccgcccc gtccttttca gacggcatga
    16261 agtatgtgaa ccgctgttta cagattgatg cccaacgctt cccacacctt caacgcatcc
    16321 gctgtcgcct tcacatcatg cacccgcacg atttgcgcgc cgcgcgctac ggaagccaac
    16381 gctgccgcca cgctgccgtg tacgcgttcc gccgcatttg cctcgccggt cagctcgcct
    16441 atcgtgcttt tgcgcgatac gccgatgagc agcggaaaac ctgtttccgc catcaattcg
    16501 ggcaaatgcc gcatcagcgc gatattgtgt tgcaagggtt tgccgaagcc ggagccgaag
    16561 ccggggtcga gtatgatgcg ttgcggtgcg atgcctgccg cgatacattc cgctgagcgc
    16621 gctttcaaat accgcgctac ttcaccgaca acatcttgat atttcggatt aatctgcatg
    16681 gttttgggca aaccctgcat gtgcatcagg caaatgcccg tgtccgcctg acgcgccagc
    16741 aattcgaccg cgccctcgtc attcaacgcc gccacatcat taataatatc gatgccgccg
    16801 agtgccaacg ctttttccat aatcaccgtg cggcgcgtgt ccaaactgat gggaacgccc
    16861 caccccgcca cttccgccaa aacaggctca acccgcgccc attcttcttc aggcgaaaca
    16921 taatccgcac ccgaccgcgt cgattcgccg ccgatgtcga gaatgtctgc gccttctttt
    16981 agaagctgtt cggcatgtgc caaggctgtt tgggcgtttt gcgaatacac gccgccgtcg
    17041 gaaaaagaat cgggtgtgag attcacgatg cccatgattt tcggtttgtc caaaccgatt
    17101 tcaaaccgtc ctgcctgcca aacgtgtcgt gccatctgaa ctcctcccaa aataaaaaac
    17161 agattatatg ccgtctgaaa ccgtcttgtg cgcttcagac ggcaccgcta ttcgggcggc
    17221 agacggcatg ttgtccgaat gtctgctccg cctttgaatc tgccggtatg cctgctatcc
    17281 gcccgacttt tcaaaacagg ttccgacgat tccgcacgcg cctgccgcct ttgccaagcc
    17341 gtacaggatt tcctgcggca tatcgcggtt ccataatccc gtaatattcg caatcacggg
    17401 cagatggctg atttggcgga ctttcacgat ggattcgaca tccaaacggt agggatggcc
    17461 tttggtatgg ttcaatacgc ccgactcgcc cagaatcagg tggcggttgc cgcgcgagac
    17521 gacatattct gcggcattca accaatcttc ggcagaatgg tgcttgtctt tacacagcac
    17581 caagggaatg ttcaggtttc cggcttcatt caatacggca agatcggaca tcagcccgcc
    17641 gcccaaatac aggatgtccg cccccgcatt caaagccgct tcgacatggc ggacgttgcg
    17701 gacgcgcacc aatacgggtt tccctgcatc atgcgccgat gcggtctgtt ccgccaaccg
    17761 tctgcaccgt ccccgccctt catccgcact tgaagtgtcg tataagtttg ccgaagtgaa
    17821 aaacggatcc agaaacactg catccgcatt gcgccatact gacggttctg cggcgatacg
    17881 gacggtttcc ccgccgccga aagccacgcc tttggcggca acgcggctgt cttccgcccg
    17941 attttcccga ctgacggttt tccatgtatc caaaatgcgg acggctttct cgacctccgg
    18001 cagcgtctgc acctccctga cgctcaaaac cctatcgtcg ccgattgcgc cgatgacagt
    18061 acgctcgtcg ccgtgagaaa tgtgttctcg cagacctctg ctgcggataa aggcgacaac
    18121 gccggcaatg tccgcttcgg cggcacgcct gctcatgaca ataatcatat ttcctcctga
    18181 cacaagaaac ggcctaccca aaataggatt tttgcaagcc gtgttatact gtggcgtgtt
    18241 ttacagattg ttcgggctat ggatttatta tcggttttcc acaaataccg tctgaaatat
    18301 gcggtggccg tgctgacgat actgcttttg gcggcagtcg ggctgcacgc ttccgtatat
    18361 cgcaccttca cgcctgaaaa catccgcagc cgcctacaac aaagcattgc acacacacac
    18421 cggaaaatct cgtttgatgc ggacattcag cgcaggctcc tgccccggcc gaccgtcatc
    18481 ctgaaaaacc tgaccattac cgaacccggc ggcgaccaga ctgccgtttc cgtccaagaa
    18541 accaaaatcg gattgagctg gaaaaacctg tggtcggatc agatacagat tgaaaaatgg
    18601 gtggtttcga gtgcggaact tgccctgacg cgcgacggga aaggtgtttg gaacatccaa
    18661 gacctgatcg acagccaaaa acgccaagcc tcagtcaacc gcattatcgt cgaaaacagc
    18721 accgtccgcc tcaatttcct gcaggaacag cttatcctga aggaaatcaa cctcaacctg
    18781 caatcccccg attcgtcggg gcagccgttt gaaagttcgg gcatactggt ttggggaaag
    18841 ctgtccgtcc cgtggaaaag cagggggctg ttcctttcaa acggcatcgg cccgcccgaa
    18901 atctcaccgt tccattttga agcttccact tcgctggacg gacacggcat taccatttcc
    18961 accaccggca gcccttctgt ccgcttcaac gccggcggag cggatgccgc cggcctcggc
    19021 ctgcgtgcag acacttcctt ccgcaacctc cacctgaccg cccaaatccc cgcgctggca
    19081 ctcaggaaca acagcattaa aattgaaacc gtcaacggcg catttaccgc cggcggcgaa
    19141 tatgcccgat gggacggttc gttcaaactc gacaaagcca acctgcactc cggcatcgcc
    19201 aacatcggca acgccgaaat ctccggcagc ttcaaaacac cgcgccacca gaccaacttc
    19261 tccctcaatt cgccgctcgt atggacggaa aacaaagggc tggacgcgcc gcgcctgtat
    19321 gtatcgaccc ttcaggatac cgtcaaccgc ctgccgcaac cccgtttcat cagccggctc
    19381 gacggttcgc tgtccgtacc gaatctgcaa aattggaatg ccgaattaaa cggcacattc
    19441 gaccgccaaa ccgttgccgc gaaattcaga tacacacatg aagacgcacc gcatctggaa
    19501 gccgccgtcg cactgcaaaa attgaacctg accccctatc ttgacgacgt gcggcaacaa
    19561 aacggcaaaa tatttcccga caccctcgcc aagctgtccg gcgacatcga ggcgcacctg
    19621 aaaatcggaa aagtccaact tcccggcctg caactggacg atatggaaac ctacctccac
    19681 gccgacaaag gccatatcgc gctcagccgt ttcaagtcag ggctttacgg cggccatacc
    19741 gaaggcggca tcagcatcgc caacacccgt cccgccactt accgcctgca acagaatgca
    19801 agcaacatcc aaatccaacc gctgctgcaa gacctgttcg gcttccacag cttcagcggc
    19861 aacggcgacg cggtcatcga cctgaccgcg ggcggcgaaa cccgaaaaga gcttatccgc
    19921 tcgcttcagg gcagcctgtc gctaaatatt tccaacggtg catggcacgg tatcgacatg
    19981 gacaatatcc tgaaaaacgg catttcgggc aaaactgccg acaatgccgc acccagcaca
    20041 cccttccacc gattcacgct caacagcgaa atttcagacg gcatcagccg ccacatcgat
    20101 accgaactct tctccgacag cctctatgtt accagcaacg gctataccaa tctggatacg
    20161 caggaattgt ctgaagatgt ccttatccgc aacgccgtcc atccgaaaaa caaaccgatt
    20221 cccctgaaaa tcaccggcac ggtggacaaa ccgtccatta ccgtcgatta cggcaggctg
    20281 accggcggca tcaattcgcg caaagagaaa cagaaaatcc tcgaagacac cctgctggaa
    20341 caatggcagt ggctcaaacc taaagaaccg taaacatcct gcgtacaaaa atgccgtctg
    20401 aaacaccccc gcgcttcaga cggcagaccg taaaacctac aaccccaatt cctcccaaat
    20461 cccatcaatc ttagccgtaa ccgcagggtc ttttttgatg acgcgtcccc attcgcggtc
    20521 ggtttctccc ggccatttgt tggtcgcatc caaacccatt ttgccgccga gtccgctgac
    20581 ggggctggcg aagtcgagat aatcgatggg cgtgttttct accaaaacag tgtcgcgcac
    20641 ggggtccatg cgcgtggtga ccgcccagat gacttctttc cagtcgcgca cgttcacatc
    20701 gtcatccacc acgatgatga atttggtata cataaactgg cgcaggaacg accagcagcc
    20761 catcatcacg cgcttggcgt gtccggcgta ctgttttttc atgctcacca ccgccatgcg
    20821 gtaggagcag ccttcgggcg gcaggtagaa atcggtgatt tcggggaact gcttttgcaa
    20881 aagcggtacg aacacttcgt tcaacgccac gcccaaaacg gcgggttcat cgggcggttt
    20941 gcccgtgtag gtcgaatggt aaatcgggtt ttcgcgcatg gtgatgcgtt cgaccgtaaa
    21001 cacagggaaa taatcctgct cgttgtaata gccggtgtgg tcgccgtacg ggccttccaa
    21061 cgcggtttcg ttcggatgga tgacgccttc caacacgatt tctgcgcggg caggcacttg
    21121 caaatcgttg ccgatacatt tcaccagctc cgtccgcgaa ccgcgcagca gtccggcaaa
    21181 ctggtattcg ctcaaggtat cgggaacagg cgttaccgcg cccaaaatgg tggcggggtc
    21241 gcagccgagt acgacggcga cgggatacgg cgtatcggga ttgagtttgc ggaactcctg
    21301 ataatccaac gcgccgccgc gatgcgacag ccaacgcata atcagcttgt ttttgccgat
    21361 gagttgttgg cggtaaatgc cgagattttg gcgttttttg tgcggcccgc gcgtgacggt
    21421 caagccccac gttaccagcg gcgcaacgtc ttccggccag caatgctgaa tcggaagttg
    21481 atacaaatca acgtcttcgc cttcccacac gatttcctga cacggcgcgt ttttcaccac
    21541 gttcggcgcc atgctccaaa tgtctttcag cagcggcagt ttggaaaacg catctttgat
    21601 gcctttgggc ggttcgggtt ctttcaaata cgccagcgtc tgcccaattt cacgcagctt
    21661 ggacacgctg tccgcgccca tgcccatcgc cacacgttcg ggcgtgccga acaggtttgc
    21721 caacacggga taaccgtagc gcgtaccgtc gggcttaatc gggttttcaa acagcaacgc
    21781 cggcccttcg gcacgcagca cgcggtcggc gatttcggtc atttccaaat acggggaaat
    21841 ggggtgtgcg acgcgcttga gtttgccctg ctgctcgagc atggcgatga agtcgcgcag
    21901 gtctttgtat ttcatattca tcctttttgt ccttttatcc tgaacaatcc gattcggata
    21961 ccgcccctat ccttgcctgt gcctcggcat attctatgcc gtgataaaag tcgcgtacca
    22021 gcggatgttc gccgccttga tggagttgca acaaaggacg ttgaccatcg ggttgggtaa
    22081 cgacattgca gtgcagaccg aaggtgtcgg tttcataagg gggcagctgg ttgcagatca
    22141 tgccgaaata aacagcgttt tcaaggttgt cgtaaaagcg gctttgatag tcgttaaaac
    22201 tcttttcgct gacggatacc cacacgccat attccagcgt ttcctcatgc ccgataatcg
    22261 gaatcggcag caccgcgcgg ataaagcggt cggtttggtc gggatagcgg atgatgcaga
    22321 aatcagaatc gcattctgct tgataagcaa tgcgttcttc ttcactgagt tgattatagg
    22381 gatcgggtgc ggtaaagccg attgcgggca tttcttcgtg gttttcgccg caggaagtgc
    22441 aagtgtacat aaggtttcag actttcaaaa cgagtttgcg gtaaagccat tcgccggcaa
    22501 acagcatccc catcaataca taggcaatca cgccggtata aaccgcccac caatcatatc
    22561 gccccaaccg tgccaacaaa gcggcaagcg tcccgttgat tataaaaaat acgcaccaaa
    22621 cctgcgttac ccggcgggta tagcgcacgg ctttttcagg caggtcgggc tgttgcagcc
    22681 gcgcaagttt ttcaatcacc gtctgcccgg caaacaggct gccgccgaac accgccaaca
    22741 tcatcagatt gacgaggacg ggataccaat acatcgaatc atgccgcccg aacaccaata
    22801 ctgcggcaaa aaacagtgta ataaacaaag ccgcataacg ctgttggggc agtttggcag
    22861 tcagggcgcg cagcagccac aacccgcaca tcgccgccgc cagccaaaca aaccagcccg
    22921 cctccctgcc gtatatagtg gattaacaaa aaccagtaca gcgttgcctc gccttgccgt
    22981 actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatac cacaaagcgg
    23041 gataggcaat gcttaatacg gtcagaaaaa tatgtccgaa aaaaccgggt ttcattttga
    23101 atccgcacaa atgttttcag acggcatccg ataaaaacat gccgtctgaa aaataattag
    23161 aaatacccga ttagcccgcc tgaatcttca ataccgcctg taccacgtcg ttgacggtgc
    23221 ggacattgcg gaaatcttcg gcctgcagct tgcggccggt ttcgcgcttg atgcggtcaa
    23281 tcaggtcgat ggcatcgatg ctgtcgattt ccaaatcttc gtaaagattg gtatcaggcg
    23341 taatccgttc cggttcgatt tcaaacaact cggtcagggt atcgcgcaac aaccggtaga
    23401 tttcttgttc ggtcatatgt ttcatcctta tgcttggcgg cttttgacaa aggcggcgag
    23461 tgttttgaca ttggcaaaat gttcgcgcaa gttctcctgc tcgccgtcca atcggaaacc
    23521 gaaatgtttt tgcaccgcca agcccaattc cagcgcatcg acggaatcca gtcccagccc
    23581 tccgtcgccg aacagcgcgt cttcgctgcc gatgtcggcg gcggttatat cctccaaagc
    23641 caaactgtcg ataatcagtt gtttgatttg gttttccaag tcgttcatat ggttttcctt
    23701 gtaaaatagt cctgcagata ttcattcagc cgccgcgccg caatcggcaa cggtttctcg
    23761 gcgagccagt cttgggggag gatgtcgtct ccgacggtaa tttcataccg tatccttttc
    23821 ggggggatgc ggtaccacgg ctgccccttt ttaaaattgg gcggattcat tttgatacat
    23881 acgggcgtaa tcacttcggc ataccgcagt cccaaagaaa ccgcgccccg gtgcattttt
    23941 acccgtccgt cccaccccgt cctcgttcct tcggggaaca ccagcaggct ctgcccgctg
    24001 tcaaaaaccg cctttaccgt ttccagcatt gcttccgact cttcgttcgg aatatagccc
    24061 gcacctttaa tctggctgct cattgccgga ttgtgctgca aatctttttt cacgatacag
    24121 ttcatttcgg gcacatggcc gacaagcagc accacatcca gcaaagacgg atggtttgcc
    24181 aaaatcaact gtcccgggcg gttgagtttt tcaacaccct tgaacgatac ctccaacacg
    24241 cccgaccatt tcagataagc aacgaacaaa cgccaagatg ttccgataat ccggcgcgcc
    24301 gccaattggc gggcgacaga ccctgaagtg ccgttcaaag tataaggcaa caaaaccaat
    24361 ttcatcataa tgccgccgac accgaaaatc acaaatccta accaagtcgc aaaaaaacgg
    24421 cggcaataat ccaatttatc cattccgctg ccacagccat tcacgattgc gatatacccg
    24481 gcggcattct cgactgccgt tcagcagaaa gcgcacccat tccaaaccgc tccaatatgc
    24541 ctcgggcagc ataccggctt cagacggcat atcgtccgaa gcagacaaag tcaggctgta
    24601 acgcgtccct ttggtcagaa ccatcgccaa agcataagca aacggcgcgc gagtcgccga
    24661 tacggcatat ccttccggca gcggatcgtc cgccgccaaa accaaaaccg acccgcatcc
    24721 ctcttccaac agtgatgccg cttccgccaa tgccgtttcc acaccgtcgg cacacacggc
    24781 caatgccgtc tgctcgctca tatcctgacg caatatcgac cattgccccg ccgttgcatt
    24841 gtgcaccgac aaaccgaacg aagtcggcga cacggtatgc gatttcaaca gttccaacca
    24901 caaatcgaaa ctgcgtgcca tttccccgtc gtgcgaggca taaactaccg gactgccggg
    24961 atgggcggag gcaatgtccc aagccgcgtc gcataccaaa cgcgccgcct tactcaaacg
    25021 gcggcgctgc atagcgggca ggaacggcaa ttccggcctg acatcgggca aaccgtcggc
    25081 aaaatccgga cattccgccc attttgccca ctgggccata tcgcgcattt tgctgcccga
    25141 aacccgccag gcggcgatgt cgaagtggaa ccggcaaata atcgacggca tagtttcttt
    25201 caaaaattta cactgtgccg cattctaacc aaagcctatc cccctgacaa tgccgaaatt
    25261 caaacgcatt tctgccccct ttctccgaca acgccgcccc tcggaaaacc gccagaatta
    25321 gcctgaattt acatttatca ttataatgcc cgtatttgcc agcctgccgc cgcaatatat
    25381 ggacacactg ccagaatgcc cgattaccaa caccgcctcc ctgctgccgc acagcgggcg
    25441 tatggttctg atagaccgca ttacccgata cggcgatgat tttgtcgaag caggggcaca
    25501 ggtaagcccc aatcacatcc ttttacttga cgacaaactg ccctacacgg catttatcga
    25561 actgatggca caggctgtcg gcgcgtatgc cggtatccaa gcccgaaaaa acgcacggtc
    25621 ggtccggctc ggcttcctgc tcggcacgcg caaacttgaa atcttcgccc aatccgtccc
    25681 aatcggcacg catctgctgg caacggcgca tatgtctatt caggatgccg ggggtatggg
    25741 cgtgtttgac tgcgaactgc gttggacaga cgcgccggaa acttcgtccg aaacactccc
    25801 ttcagacggc attttggcgc gcgcctcact caacgtgtac agccccgaac accctgccgg
    25861 aacaaccgat gccgtctgaa caggcacaca catacggaga acaacgatga ccgaaactgt
    25921 cctgattacc ggctccaaca ggggcatagg caaagccgtc gcattcggtt tggcggaaga
    25981 cggctttgat atcgctgtcc actgccgcag tcgccgcgac gaagccgaag ccgtggcgga
    26041 agaaatccgc gctttgggca gaaatgcgcg cgtgttgcag tttgacgtgt ccgaccgcga
    26101 agcctgccgc gagattctga ccgccgacat cgaagcaaac ggcgcgtatt acggcgtggt
    26161 gttgaacgcc ggactgacgc gcgacaatac cttccccgcg ttttcagatg acgattggga
    26221 tgtggtgctg cggactaatt tggacggttt ttacaatgta ttgcatccgc tggttatgcc
    26281 gatgatacgc cgccgcaaag ccggacggat tgtgtgtatg gcatcagtgt ccggcctgac
    26341 gggcaaccgc gggcaggtca attacagcgc gtcaaaagca ggcattatcg gcgcggcaaa
    26401 agccttggcg gtcgaactgg cgaaacgcaa aatcaccgtc aactgtgtcg cgccgggtct
    26461 catcgatacc gatattatcg atgagaacgt acctgtcgaa gaaatcttaa aggctgtccc
    26521 cgcagcgctt atggggctgc cggaagaagt ggcgcacgcg gtgcgtttcc tgatggatga
    26581 aaaagcggcg tacatcacgc gccaggtgat tgcggtgaac ggaggtttgt gttgaatacc
    26641 agaagggtcg cagtaacagg cataggcggc attaccgcct tcggccggga ttggcaaagc
    26701 atacaggcag cattcaaagc cgaaaaaaac gccgtcaaat atatggattg gcacgaacgt
    26761 ttccccgaat tggaagcgca actgggtgcg ccgattgaaa attacgcgcc gccgaaacat
    26821 tggacgcgca agcagctcag aagtatgggg cgcgtgtcgt acctgtgcgt cgatgcggcg
    26881 gagcaggcgc tggcggatgc cggtttgctc ggggacgaaa gcattaccga cggacggatg
    26941 ggcgttgcct gcggctcttc cagcggcagc accaaagaca tcggcgatgt gggcgaattg
    27001 ttgctgaccg gcacgtcgcg caacttcagc gccaacacct atgtgcgtat gatgccgcac
    27061 accaccgccg ccaatatcgg catctttttc gggctgaaag ggcgcatcat cccgacatcg
    27121 agcgcgtgtt cgtccggcag ccaaggcata ggttatgcct acgaagccat caaatacggt
    27181 ctgaccgata tgatgctggc gggcggaggc gaagaatttt tcccgtccga agtgtatgtt
    27241 ttcgactcgc tttatgccgc cagccgccgc aacggcgaac cggaaaaaac cccgcgccca
    27301 tacgacgcga accgcgacgg gctggtcatc ggcgaaggcg cggggatttt cgtgctggaa
    27361 gaattggaac acgccaaacg gcgcggtgcg ataatttacg ccgaactcgt cggctacgga
    27421 gccaacagcg atgcctacca tatttccacg ccccgccccg acgcgcaagg cgcaatcctt
    27481 gcctttcaga cggcattgca acacgcaaac cttgcacccg aagacatcgg ctggattaat
    27541 ctgcacggca ccgggacgca ccacaacgac aatatggaaa gccgcgccgt tgcagcggtt
    27601 ttcggcaaca atacgccctg cacgtccacc aagccgcaaa ccggacacac gctgggcgcg
    27661 gcggacgcaa tcgaagccgc gttcgcgtgg ggcattgccg accggcaaag caatcccgaa
    27721 ggaaaacttc cgccccggct ttgggacggg cagaacgacc ccaacctgcc cgccatcaac
    27781 ctgaccggca gcggcagccg ctgggaaacc gaaaaacgca ttaccgccag ctcgtcgttt
    27841 gccttcggag gaagcaactg cgtcttaatc atcggatgaa ataagtttgt caatcccacc
    27901 gctatgctat acaatacgcg cctactcttg acgggtctgt agctcagggg ttagagcagg
    27961 ggactcataa tcccttggtc gtgggttcga accccaccgg acccaccaat tcccaagccc
    28021 ggacgtatgt ttgggctttt ttgccgccct gtgaaaccaa aatgctttga gaaaccttga
    28081 taatgaaaaa agtcagcgta ttgattgttg ccaaaaacga agcaaaccac attcaggaat
    28141 gtattgaaag ttgccgtttc gataaagaag ttatcgttat cgacgactac agcaccgaca
    28201 atactgccga aattgccgag ggtttgggcg caaaagtctt cagacggcat ttgaatgggg
    28261 atttcggagc gcaaaaaaca tttgccatcg aacaggcagg cggagaatgg gttttcctga
    28321 ttgatgcaga cgaacgctgc acgccggaac tatctgatga aatctcaaaa attgtccaaa
    28381 ccggcgatta tgccgcctat tttgtcgaac gccgcaacct tttccccaac catcccgcca
    28441 cacacggcgc gatgcgtccc gacagcgtat gccgtctgat gccgaaaaaa gacagttcgg
    28501 tgcaaggcaa agtacacgaa accgtacaaa ccccctaccc caaacgccgt ctgaagcatt
    28561 ttatgtacca ttacacgtac gacaactggg aacaatattt caacaagttc aacaaatata
    28621 cttccatttc agccgaaaaa taccgagagc agggaaagcc cgtgcgtttc gttagggaca
    28681 ttatcctccg cccgatttgg gggtttttca aaatttatat cctgaacaaa gggtttcttg
    28741 atggaaaaat gggttggatt atgtccgtca accacagcta ttacacgatg attaaatatg
    28801 tcaaactata ttatctgtac aaatccggcg gaaaatttta aatggaaaaa gaattcagga
    28861 tattaaatat cgtatcggcc aagatttggg gtggaggcga acaatatgtc tatgatgttt
    28921 caaaagcatt ggggcttcgg ggctgcacaa tgtttaccgc cgtcaataaa aataatgaat
    28981 tgatgcacag gcgattttcc gaagtttctt ccgttttcac aacgcgcctt cacacgctca
    29041 acgggctgtt ttcgctctac gcacttaccc gctttatccg gaaaaaccgc atttcccacc
    29101 tgatgataca caccggcaaa attgccgcct tatccatact tttgaaaaaa ctgaccgggg
    29161 tgcgcctgat atttgtcaaa cataatgtcg tcgccaacaa aaccgatttt taccaccgcc
    29221 tgatacagaa aaacacagac cgctttattt gcgtttcccg tctggtttac gatgtgcaaa
    29281 ccgccgacaa tccctttaaa gaaaaatacc ggattgttca taacggtatc gataccggcc
    29341 gtttccctcc ctctcaagaa aaacccgaca gccgtttttt taccgtcgcc tacgccggca
    29401 ggatcagtcc agaaaaagga ttggaaaacc tgattgaagc ctgtgtgata ctgcatcgga
    29461 aatatcctca aatcaggctc aaattggcag gggacggaca tccggattat atgtgccgcc
    29521 tgaagcggga cgtatctgct tcaggagcag aaccatttgt ttcttttgaa gggtttaccg
    29581 aaaaacttgc ttcgttttac cgccaaagcg atgtcgtggt tttgcccagc ctcgtcccgg
    29641 aggcattcgg tttgtcatta tgcgaggcga tgtactgccg aacggcggtg atttccaata
    29701 ctttgggggc gcaaaaggaa attgtcgaac atcatcaatc ggggattctg ctggacaggc
    29761 tgacacctga atctttggcg gacgaaatcg aacgcctcgt cttgaaccct gaaacgaaaa
    29821 acgcactggc aacggcagga catcaatgcg tcgccaaccg ttttaccatc aaccataccg
    29881 ccgacaaatt attggatgca atataaactg ctttcagacg gcatatgccg tctgaaagcc
    29941 tttgatgcaa caaaccgcca aattatattc gttcattgga aagaaacacc ccgaatccat
    30001 ccttcaaaat aagaaaatcc caatatcccc cgatattacg cagcctattg gcaaagtttt
    30061 gcagcgtctt ccccggcttg tgctgccgca tcaagtgctt tgttacaatg tatagtaga
//
LOCUS       DAWGRZ010000006        26971 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000006 DAWGRZ010000000
VERSION     DAWGRZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26971
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.006"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(92..880)
                     /locus_tag="WHT44_000182"
     CDS             complement(92..880)
                     /locus_tag="WHT44_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ2532423.1"
                     /translation="MNPQDKCWQVHRHLAEYADQRLTLVRNAPKYILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGGFWQRFTGKGVVQHCQSPIAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWACALKTDGLLFFTCFGRDTFTELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMAWNDENAA
                     RSCVGTIFEREGGLGITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            complement(882..1796)
                     /gene="bioH"
                     /locus_tag="WHT44_000183"
     CDS             complement(882..1796)
                     /gene="bioH"
                     /locus_tag="WHT44_000183"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ2532424.1"
                     /translation="MCLKHRGQLFAASVRYAAKSSRQPAQMPSETPDEPWHNTQRLIV
                     GASAMRRQRERKSMPDAVKKVYLIHGWGANRHMFDDLMPRLPATWPVSAVDLPGHGDA
                     PFVRPFDIAAAADGIAAQIDAPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARL
                     TAAEDYPEGLAAPALGKMVGAFRSDYAKHIKQFLQLQLLHTPDADGIIGRILPDLARC
                     GTPSALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLV
                     VMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            1852..2349
                     /locus_tag="WHT44_000184"
     CDS             1852..2349
                     /locus_tag="WHT44_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ2532425.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLAD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKG
                     GERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELAKTLKKSGANRICCWTLAR
                     TPMKK"
     gene            2414..2878
                     /gene="trmL"
                     /locus_tag="WHT44_000185"
     CDS             2414..2878
                     /gene="trmL"
                     /locus_tag="WHT44_000185"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ2532426.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            2838..3011
                     /locus_tag="WHT44_000186"
     CDS             2838..3011
                     /locus_tag="WHT44_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ2532427.1"
                     /translation="MKRGGNTVTQAAFERRFMPSENLSGHIPNRRPHCTAVRNVSGGH
                     DAHSSSTQERNTF"
     gene            3008..3727
                     /locus_tag="WHT44_000187"
     CDS             3008..3727
                     /locus_tag="WHT44_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ2532428.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVNQGTAHVKIEQIVPGQSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAVSSSGTNLSVEKRRYEYVVKMGPFTSQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            3789..4115
                     /locus_tag="WHT44_000188"
     CDS             3789..4115
                     /locus_tag="WHT44_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ2532429.1"
                     /translation="MNIKHLLLTSAATALLSISAPALAHHDGHGDDDHGHAAHQHNKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            4197..4781
                     /gene="ruvA"
                     /locus_tag="WHT44_000189"
     CDS             4197..4781
                     /gene="ruvA"
                     /locus_tag="WHT44_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ2532430.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            4781..4918
                     /locus_tag="WHT44_000190"
     CDS             4781..4918
                     /locus_tag="WHT44_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532431.1"
                     /translation="MPPEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            4944..6800
                     /locus_tag="WHT44_000191"
     CDS             4944..6800
                     /locus_tag="WHT44_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ2532432.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVAWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPRALPLNVPQGAIKFEHVDFSYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            6805..7728
                     /gene="rsgA"
                     /locus_tag="WHT44_000192"
     CDS             6805..7728
                     /gene="rsgA"
                     /locus_tag="WHT44_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ2532433.1"
                     /translation="MPSEHPFSDGIPTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIEAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAQTGAASPERLAFLQGITDELPG"
     gene            7975..8199
                     /locus_tag="WHT44_000193"
     CDS             7975..8199
                     /locus_tag="WHT44_000193"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ2532434.1"
                     /translation="MKKNAPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDTDGLKELNLESDE"
     gene            8183..9079
                     /locus_tag="WHT44_000194"
     CDS             8183..9079
                     /locus_tag="WHT44_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ2532435.1"
                     /translation="MNPTNDLKTWQQRAQAQTELLLERFLPSEGEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSRGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGATACPDLSDAELSVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(9188..9487)
                     /locus_tag="WHT44_000195"
     CDS             complement(9188..9487)
                     /locus_tag="WHT44_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ2532436.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHMIELA
                     AGFALTAILAYILESRAGSVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(9599..11044)
                     /gene="nuoN"
                     /locus_tag="WHT44_000196"
     CDS             complement(9599..11044)
                     /gene="nuoN"
                     /locus_tag="WHT44_000196"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ2532437.1"
                     /translation="MNWSDLNLMPAMPEIVLLALLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALMFTLFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMGVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARPAAGGNNAAKF
                     LLSINALLLVLWGIMPQTVIDWCAKALENTL"
     gene            complement(11054..12550)
                     /locus_tag="WHT44_000197"
     CDS             complement(11054..12550)
                     /locus_tag="WHT44_000197"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ2532438.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLSTGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFAAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVNGQLNDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFTILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            complement(12639..14663)
                     /gene="nuoL"
                     /locus_tag="WHT44_000198"
     CDS             complement(12639..14663)
                     /gene="nuoL"
                     /locus_tag="WHT44_000198"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ2532439.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHSLGKNDNPHESPLVVTLPLILLAIPSVIIGYVAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQTFRPIYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            complement(14712..15053)
                     /locus_tag="WHT44_000199"
     CDS             complement(14712..15053)
                     /locus_tag="WHT44_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532440.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVFPK"
     gene            complement(15177..15752)
                     /locus_tag="WHT44_000200"
     CDS             complement(15177..15752)
                     /locus_tag="WHT44_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEZ2532441.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKIVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            complement(15786..16091)
                     /gene="nuoK"
                     /locus_tag="WHT44_000201"
     CDS             complement(15786..16091)
                     /gene="nuoK"
                     /locus_tag="WHT44_000201"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ2532442.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            complement(16088..16759)
                     /locus_tag="WHT44_000202"
     CDS             complement(16088..16759)
                     /locus_tag="WHT44_000202"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ2532443.1"
                     /translation="MTFSAILFYILAAIVLYGAVRTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVYRKTTNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKPE
                     GEGKA"
     gene            complement(16771..17250)
                     /gene="nuoI"
                     /locus_tag="WHT44_000203"
     CDS             complement(16771..17250)
                     /gene="nuoI"
                     /locus_tag="WHT44_000203"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ2532444.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            complement(17331..18407)
                     /gene="nuoH"
                     /locus_tag="WHT44_000204"
     CDS             complement(17331..18407)
                     /gene="nuoH"
                     /locus_tag="WHT44_000204"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ2532445.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVILGVWMISPLNLWK"
     gene            complement(18410..20671)
                     /locus_tag="WHT44_000205"
     CDS             complement(18410..20671)
                     /locus_tag="WHT44_000205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ2532446.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGSLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGKQNQVGVWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLASSKEELFMPLLSQEAAHPDEWAGRLKNLSVNAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAVYAAAQELADATGAVLGILPQAANSVGADVLNVNSGKSV
                     VEMVNAPKQAVLLLNVEPEIDTADGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPLWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDVESLPSKLDNRNAWTGEGVQTTSDRLVRVGGVGIYHTDSIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            complement(20983..21264)
                     /locus_tag="WHT44_000206"
     CDS             complement(20983..21264)
                     /locus_tag="WHT44_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ2532447.1"
                     /translation="MRNHPLTRQPAVYILANGKNGTLYIGVTMNLPERVWQHKNHVNI
                     DGFTARYDVHDLVWYQFFENMPEAVAKEKTMKKWRREWKIKLIEEQNTE"
     gene            complement(21508..21945)
                     /locus_tag="WHT44_000207"
     CDS             complement(21508..21945)
                     /locus_tag="WHT44_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532448.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRGNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            complement(21945..23246)
                     /gene="nuoF"
                     /locus_tag="WHT44_000208"
     CDS             complement(21945..23246)
                     /gene="nuoF"
                     /locus_tag="WHT44_000208"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ2532449.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            complement(23636..24109)
                     /gene="nuoE"
                     /locus_tag="WHT44_000209"
     CDS             complement(23636..24109)
                     /gene="nuoE"
                     /locus_tag="WHT44_000209"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ2532450.1"
                     /translation="MLSAESLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            complement(24109..25365)
                     /gene="nuoD"
                     /locus_tag="WHT44_000210"
     CDS             complement(24109..25365)
                     /gene="nuoD"
                     /locus_tag="WHT44_000210"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ2532451.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLVGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYEGSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            complement(25355..25948)
                     /locus_tag="WHT44_000211"
     CDS             complement(25355..25948)
                     /locus_tag="WHT44_000211"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ2532452.1"
                     /translation="MASIQDLYETVSRVLGNQAGKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEAWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            complement(25961..26443)
                     /locus_tag="WHT44_000212"
     CDS             complement(25961..26443)
                     /locus_tag="WHT44_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ2532453.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            complement(26434..26790)
                     /locus_tag="WHT44_000213"
     CDS             complement(26434..26790)
                     /locus_tag="WHT44_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ2532454.1"
                     /translation="MLSAYFPVFVFILIGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
BASE COUNT         6714 a         7577 c         6812 g         5868 t
ORIGIN      
        1 cactatacat cggcatttcc tttatttctg tcggttttca cagacaaatg ccgtctgaaa
       61 cctggaaacg gcttcagacg gcatctgcac atcatctctt cggaaaaaac tgcaccacgt
      121 tctccccttg cggcagcatc agttttttca cggcgtgtcc gtacaccgtt tccagcgtaa
      181 tgcccaaacc gccttcccgc tcaaatattg tcccgacaca cgaacgcgcg gcgttttcat
      241 cgttccacgc catcgcccgc caaacgccca gcgtgtccat atccgcccaa aacgtttccg
      301 cctttttgta atccaacacc agcttcgccg tatcggtaac ggggtcgtaa aagccgtttt
      361 cagcaagcat atcgcccaag tcgtgcatat cgggaaacat cacgctgcgg ctttcaatgc
      421 cgttttcttt cagacggcat ttcagttcgg taaaagtgtc gcgcccgaag caggtaaaaa
      481 acagcagccc gtccgtcttc aaggcgcacg cccagttgtg cagcacagga aggatttgtt
      541 ccgccgccaa cagtccgaga ttcgaccaca acatatcagc acacgcttcg ggcagcggcg
      601 cgatcgggga ttggcagtgt tgcaccacgc ccttacccgt aaacctttgc caaaaaccgc
      661 ctttgcgggc ggcagcggca gccgccaaaa agtccgcacg ggaatcgtat tcttcaaata
      721 ccgcctgcgg atagcgtttc gccagcaggc tgcggctgat gtccgcatcc gcaccggcaa
      781 gcaggatata cttgggcgcg ttgcggacga gtgtcaggcg ttggtcggca tattcggcga
      841 gatggcggtg aacctgccag catttgtcct gcggattcat gtcaaacccc ttcaacaaag
      901 tcgcggtaca gcgcggcaaa cgcttccgca tggctcaaaa acggcgcgtg tgccgccttt
      961 tccatcacaa ccagcctgct gcctttcaaa tgccggtgca gatactcgcc catacgcggc
     1021 ggcgtaatcg cgtctttgcc gccgaacacc agcagtaccg gaacatctat cttgtccaac
     1081 aaatgccgcg catccgccct ttccgccgcg tccaaagctt cctgtaaggc ggagggcgtg
     1141 ccgcaccgtg ccaaatcggg caggattctg cctatgattc cgtccgcatc aggcgtgtgc
     1201 agaagctgga gttgtagaaa ctgtttgata tgtttggcat aatccgaacg gaacgcgccg
     1261 accattttgc ccagtgcagg cgcggcaagc ccttcgggat aatcttcggc agccgtcagc
     1321 cgtgcgaaac tcgccgtcag gcagagcgaa cggactttgt cgggatggcg cgccgccaga
     1381 tacagcgcga ccaatccgcc gagcgaccag ccgagaatgt cggccggagc gtcaatttga
     1441 gcggcaatgc cgtcggccgc agccgcaatg tcgaaaggtc ggacaaacgg agcgtccccg
     1501 tgtccgggca aatcgacggc ggacaccggc cacgttgcag gcaggcgcgg catcaaatcg
     1561 tcgaacatgt ggcggttcgc cccccaaccg tgtatcaggt aaactttttt gacggcatca
     1621 ggcatggatt ttctctctcg ttggcggcgc atcgcagacg cgcccactat caggcgttgc
     1681 gtattatgcc acggttcgtc tggcgtttca gacggcatct gcgccggctg ccgcgacgat
     1741 ttggcggcat accgcaccga cgcggcaaac agctgccccc tgtgtttcag acacatccaa
     1801 ggcggatcgg tgtgcggcgg ctgtcagaaa aaaccgcccg cattcgaccg gatgtgggct
     1861 tcgctgcatt acgaaccgcc cgtcagcaat atgatacacg cgctgaagca cttggctgat
     1921 ttgagcatgg tgcagccgct ggcagacctg atgatgcaga atccgcccga ccggcttgca
     1981 gacgaatgtt tcgatttcgt cctgcccgta ccgctaagca gggagcggct gctgcaacgc
     2041 gggttcaacc aaagtgaaag catcgtcggg ctgctggcac aacgctacgg ctggcagata
     2101 ctgccccgac acaccgtttt ccgacaccac cgcccgccgc aaagcacgct caaaggcggc
     2161 gaacggcggc gaaacatcaa aaacgccttt gaaatccgca caccgatacc ggaaaactgt
     2221 aatattctgt taatcgacga tgtctttacc accggcgcga cgctggacga attggcaaag
     2281 acgctgaaaa aatcgggcgc aaaccgaatc tgctgctgga cgctggcacg cacgccaatg
     2341 aaaaaataac taaatttttt gacaccccat ccgccttgtg gcaaggttac gcgcctataa
     2401 gtgattgata tttatgttta ccatcgtttt ataccagccg gaaatcccgc cgaacacggg
     2461 caacatcatc cgcctgtgcg ccaataccgg cgcggatttg caccttgtca aaccgctcgg
     2521 cttcccattg gattccgcca aaatgaaacg cgccgggctc gactaccacg agttcgccag
     2581 cctgacggtg cacgaaaact tcgacgactg cctcaaggca ctcgcaggca ggcgcatttt
     2641 cgccctgacc accaaaggca cggcgcgccc cgatgaaacc gcgtttcaaa aaggcgacgt
     2701 tttactgttc gggccggaaa cgcgcggact gcctgccgac atcctcgaca gcctgcccgc
     2761 cgcgcaaaaa atccgcctgc cgatgcagcc cggcagccgg agtatgaacc tttccaacac
     2821 cgtcgccgtg attctctttg aagcgtggcg gcaacacggt tacgcaggcg gcgtttgaac
     2881 gcaggttcat gccgtctgaa aacctatccg gacacattcc gaaccgccgt ccgcactgta
     2941 cggcggttcg gaacgtatcc ggcgggcatg atgcccattc gtcttcaact caagaacgga
     3001 acacgttttg actttaaccc gaaaaaccct tttccttctc accgccgcat tcggcacaca
     3061 ctcccttcag acggcatccg ccgacgcagt ggtcaaggca gaaaaactgc acgcctccgc
     3121 caaccgcagc tacaaagtcg ccggaaaacg ctacacgccg aaaaaccaag tcgccgaatt
     3181 cacgcaaacc ggcaacgcct cgtggtacgg cggcaggttt cacgggcgca aaacttccgg
     3241 cggagaacga tacgatatga acgcctttac cgccgcccac aaaaccctgc ccatccccag
     3301 ctatgtgcgc gtaaccaata ccaaaaacgg caaaagcgtc atcgtccgcg tcaacgaccg
     3361 cggccccttc cacggcaacc gcatcatcga cgtatccaaa gccgccgcgc aaaaattggg
     3421 ctttgtcaac caagggacgg cacacgtcaa aatcgaacaa atcgtcccgg gccaatccgc
     3481 accggttgcc gaaaacaaag acatctttat cgacttgaaa tctttcggta cggaacacga
     3541 agcacaagcc tatctgaacc aagccgccca aaacttcgcc gtttcgtcat cgggtacgaa
     3601 cctctcggtt gaaaaacgcc gttacgaata cgtcgtcaaa atgggaccgt ttacctcgca
     3661 ggaacgcgcc gccgaagccg aagctcaggc gcgcggtatg gttcgggcgg tattgaccgc
     3721 cggctgacgg ttatttgata ccttattaat atccacattt tccaacccac gatttacaaa
     3781 ggcaaaatat gaacatcaaa caccttctct tgacctccgc cgcaaccgca ctgctgagca
     3841 tttccgcccc cgcgctcgcc caccacgacg gacacggcga tgacgaccac ggacacgccg
     3901 cacaccaaca caacaaacaa gacaaaatca tcagccgcgc ccaagccgaa aaagcagcgt
     3961 tggcgcgtgt cggcggcaaa atcaccgaca tcgatctcga acacgacaac ggccgtccgc
     4021 actatgatgt cgaaatcgtc aaaaacggac aggaatacaa agtcgttgtc gatgcccgta
     4081 ccggccgcgt gatttcctcc cgccgcgacg actgaatttg atacaatccg tgccgtctga
     4141 agcccaaacc ggtttcagac ggcattttgc acccgacact tcaggattcg gcacacatga
     4201 tcagcagact gaccggcaaa ctggttgaaa aaaaccctcc gcaaatcgtc atcgatgtca
     4261 acggtgtcgg ctatgaggcc gacgtatcga tgcagacctt ctacaacctg ccgcccgtgg
     4321 gtgaaagcgt acaactgttt acccagctta tcattcggga agacgcacat cttttatttg
     4381 gttttgccac tgcggaagaa cgcaagacct tccgccaact gatcaaggtc ggcggcatcg
     4441 gcgcgaaaac ggctttgggc attttgtcgg caatgactgc cgacgaactg gcgcgggcgg
     4501 ttgcagaaga agatgtcaaa cgcctctcct ccgccccggg aatcggcaaa aaaaccgccg
     4561 aacgtatggt tttggaactg cgcggcaagc tggtcgcgca tacggtaacg gacgggctgt
     4621 ttgccgccgc acccgccgcc gacgaaacgg aagacatcgt cagcacgctg cttgcgctgg
     4681 gttacagcga acgcgaagca aaagcggcgg tcaaaggcgt tccggagggg acggacgtgg
     4741 gcgaaggcgt gcgccttgct ttgaaaaacc tgctgaaata atgccgcctg aaggcggcgc
     4801 ggcgtttgcc ctgacgaaac cctgcgtttc cgcctcgcgc tgtcttcccg acggcttttc
     4861 ccgtaaaatg gcgttattgt ccttcctttc agacggcatt gtgtttgatg ccgtctgaaa
     4921 ctgttttacc gaaatcgaaa acaatgttga acaaaatatt ttcctggttc gagtcccgaa
     4981 tcgaccctta tcccgaagcc gccccgaaaa cgccagaaaa aggcttgtgg cggtttgtct
     5041 ggagcagcat ggccggcgtg cggaaatgga tagccgccct ggctgcgctg accgccggca
     5101 tcggcattat ggaagccctg gtttttcaat ttatgggcaa aatcgtggag tggctcggca
     5161 aatacgcgcc cgccgaactg tttgccgaaa aaggttggga actggcggca atggcggcga
     5221 tgatggtatt ttcggttgcg tgggcgtttg ccgcgtccaa cgtgcgcctg caaacccttc
     5281 agggcgtgtt ccccatgcgc ctgcgctgga acttccaccg cctgatgctg aaccaaagcc
     5341 tcggttttta tcaggacgaa tttgccggac gcgtgtccgc caaagtcatg cagaccgcgc
     5401 tggcgttgcg cgacgcggtg atgacggttg ccgatatggt cgtttatgtg tcggtgtatt
     5461 tcattacctc cggcgtgatt ctcgcctcgc tcgactcatg gctgctgctg ccctttatcg
     5521 gctggattgt cggtttcgct tcggtgatgc gcctgctgat tcccaaattg gggcaaaccg
     5581 ccgcatggca ggcggatgcc cgctcgctga tgaccggccg cattaccgat gcctattcca
     5641 atatcgccac cgtcaaactc ttctcccacg gcgcgcgtga agccgcctat gccaagcagt
     5701 cgatggaaga atttatggtt acggtgcgcg cccaaatgcg gctggcgacg ctgctgcatt
     5761 cgtgcagctt catcgtcaac acctccctga ccctctccac cgccgcactg ggcatctggc
     5821 tctggcacaa cgggcaggtc ggcgtgggcg cggttgctac agccaccgcc atggcgttgc
     5881 gcgtcaacgg tttgtcgcaa tacattatgt gggaatccgc gcggctgttt gaaaacatcg
     5941 gcaccgtcgg cgacggcatg gcaaccctgt ccaaaccgca caccatcctc gacaagcccc
     6001 gggcactgcc gctgaacgtg ccgcaaggcg caatcaaatt tgaacacgtc gatttctcct
     6061 acgaagcggg caaaccgctg ctcaacggct tcaacctcac catccgcccg ggcgaaaaag
     6121 tcggcttgat cggacgcagc ggcgcgggca aatccaccat cgtcaacctg cttttgcgct
     6181 tctacgaacc gcaaagcggc acggtttcga tcgacgggca ggacataagc ggcgttaccc
     6241 aagaatcttt acgcgcccaa atcggtttgg tcacgcaaga tacctcgctg ctgcaccgtt
     6301 ccgtgcgcga caacattatt tacggccgcc ccgacgcgac cgatgccgaa atggtttctg
     6361 ccgccgaacg cgccgaagcc gccggcttca tccccgacct ttccgatgcc aaagggcggc
     6421 gcggctacga cgcacacgtc ggcgaacgcg gcgtgaaact ctccggcggg caacgccagc
     6481 gcatcgccat cgcccgcgtg atgctcaaag acgcaccgat tcttcttttg gacgaagcca
     6541 ccagcgcgct cgattccgaa gtcgaagccg ccatccaaga aagcctcgac aaaatgatgg
     6601 acggcaaaac cgtcatcgcc atcgcccacc gcctctccac catcgccgca atggacaggc
     6661 tcgtcgtcct cgacaaaggc cgcatcatcg aagaaggcac acacgccgaa ctcctcgaaa
     6721 aacgcgggct ttacgccaaa ctctgggcgc accagagcgg cggcttcctc aacgaacacg
     6781 tcgagtggca gcacgactga accgatgccg tccgaacacc cgttttcaga cggcattccc
     6841 acacccaacc ccaaagaaac catgaacgac accgcccaaa ttaccgccag ctacggccgc
     6901 cgctacattg tccgcacgcc cgacggcaca acctacgaag ccagcacccg caaaaaacgc
     6961 gtcgatttcg cctgcggcga ccgcgtccgc atcagccccg tcaacgccga acaagttgtg
     7021 attgaagatt ttttaccgcg ccaaagcctg ctctaccgcc aagacgcgtg gaaaaccaaa
     7081 ctcatcgccg ccaacgttac ccaactcctc atcgtaaccg ccgccgtccc gagtccgagc
     7141 gtgcggctgc tgcaacgcgc cctgcttgcc gccgaagccg ccggtattga agccgtcatc
     7201 gtcctgaaca aagccgacct gcccgaaacc gccctttggc gcgaaaaact caaattctac
     7261 gaaacgctgg gttatcccgt catcgaaacc cgcgcactgg aaaacgccgg cagcctgcgc
     7321 cccgccctgc aagggcacag caacatcctg ctcgggcaga gcggtatggg caaatccacc
     7381 ctgaccaacg cccttttggg cagccaaacc gcccgcaccg gcgacatttc cgccgcactc
     7441 gactcgggaa aacacaccac cacccacgcc cggctttatg atttgaacgg cgaaacccaa
     7501 ctcatcgact ccccgggttt gcaagaattt ggtttacacc acctccaagc cgccgatttg
     7561 ccgcgctatt tccccgattt ccgccacctt gtcgggcaat gccgcttcca caactgcacc
     7621 caccgcgccg aacccggctg cgccttcaaa gccgccgcgc aaaccggggc ggcaagcccc
     7681 gaacgcctcg cctttttgca gggcatcacc gacgaactgc ccgggtaacg ccttgccgcc
     7741 tgggcggaaa aaatgccgtc tgaagccgga ttcgggtttc agacggcatc cgtttttcaa
     7801 aaatgctaca atccgctttt taccggaaca tccgaaacta tgttccaaca cacaggacgg
     7861 cacataaagc accgccctat gtattgccct gatttggaaa gggttacacc cctcccaaat
     7921 aaagtctgat cctgccgccc taaagggcgg ggtttcaacc gaaaaggaaa tacgatgaag
     7981 aaaaacgccc cgaaatcgtt tgaagaagcc ttgtcgcgcc tcgaatcgct gacgcagtct
     8041 atgcagggcg aaatgccctt ggaagacgcg cttgccgcct atcaggaagg caacgagctg
     8101 gtcaggtact gccaaaccaa actggcacaa gtcgaacaaa agctacaggt tttagacaca
     8161 gacgggctga aggagttaaa ccttgaatcc gacgaatgat ttgaaaacgt ggcaacagag
     8221 ggcgcaggcg caaacagagc tgctgcttga acggttttta ccgtctgagg gggaaatacc
     8281 gcacacactg cacgaagcga tgcgttatgc ggctttggac ggcggtaagc gtctgcggcc
     8341 gatgctggtt ctggcagctt cggaattagg cgaagccgtg catgaagcag tagaacaggc
     8401 aatggcggca atcgaaatga tccacgtcta ttctttggtt cacgacgata tgccggcgat
     8461 ggacaacgac agcctgcggc gcggcaaacc gacttgccac atcaaatatg gcgaagcgac
     8521 cgcccttctg accggcgacg ctttgcagac acaggcattt gacgtgttga gccgtccgac
     8581 agaactgccc gccgcacgcc agttggcaat gttgtcggtg ttggcgaaag cgggcggcag
     8641 caggggtatg gcgggcggtc aggcaatcga tttggcgaat gtcggcaaac aaatggttca
     8701 agccgatttg gaacagatgc acagcctgaa aacgggtgcg ctaatccgtg cggcggtttt
     8761 attgggggcg acggcgtgtc ctgatctgtc agatgcggaa ctttccgtat tggacgctta
     8821 cgcggcaaaa ctggggctgg cgttccaagt cattgacgat gtgttggatt gtgaagcgga
     8881 cacggcgact ttgggcaaga cggcgggcaa agatgcagac aacgacaagc cgacttatgt
     8941 gaaactgatg ggcttggaag cggcgcgctc atacgcacac aaactggttg ccgaagcggt
     9001 cgcgctgctc gaacccttcg gcgacaaagc cctgcgcctg cggcagttgg cagaatttgc
     9061 agtcgcccgc aaatattaaa accggcgtat atcgccggct gaaacgtgcc ggcacagcga
     9121 tgccggcctg cacttgaatg aaacaaatgc cgtctgaaca ggtttcagac ggcatttcta
     9181 tgcttgtcta ttccctgttg cgcgtgtgcc aaaaataccg ccacacaaaa catggaaacg
     9241 caaaaatcag gtacaggcag acgactgtgg cataaaactc ccaaccctga tcgtgtaccg
     9301 atcctgcacg ggattcgaga atataggcaa gaatggcggt cagtgcgaaa cctgccgcca
     9361 gctcgatcat gtggtgtccg aaatgtttgc gcttgagcgc ggccacgccg aacagtctgg
     9421 tggtgaggaa gggggcgttg gcaaagatga gtgccaagac caaaaggatg tacatggatg
     9481 cggtcatggt gggctttctg tttgggcatt gtcggcggcg atgccgtctg aactgcattg
     9541 cggaacggca tatcttaaca aaaacggcag cctctgacac ggctgccgtt gcggcggctt
     9601 acagcgtgtt ctccaacgcc ttggcgcacc agtcgataac ggtttgcggc ataatgcccc
     9661 acagcaccaa cagcagcgcg ttgatgctta agaggaattt tgcggcattg ttgccgccgg
     9721 cggcgggacg ggcgcggccg gattcgtcaa aatacatgac tttgaccacg cgcaggtagt
     9781 agaacgcgcc aatcagcgac atcacgacgg caaatacgga caaccaaaca tagccttgtt
     9841 ttaagagtgc cataatcacg ccgaatttgg cgtaaaagcc catcagcggc ggaatgcccg
     9901 ccatagagaa cataaccagc agcatcaaaa aggcaagcca tacgcggtgt tggttcaacc
     9961 ctgccaaatc gctgatgttt tcgcactcgt tgtccccgtc cgacaacacc atcaacacac
    10021 cgaaccctgc cgccgccatc agcgcgtagg taatggcgta atagagaccc gccgcaaagc
    10081 cgaccgcgcc cgccataaac gccagcagga taaaccccat atgcgatacg gtggaatagg
    10141 cgagcatacg tttgatattg gtctgcatga tggcggcaag gttgccgacc agcagcgagg
    10201 cggcggcaag cagggcaaac atcagagacc agtcatgatg cacggttccc aacccggtaa
    10261 cgaggatgcg gaaagcgaaa acaacggcgg cgattttcgg ggcagtgccg accagggcgg
    10321 taacagaagt gggcgcgccg tgatacacgt cgggcaccca catatggaac ggcaccgcgc
    10381 cgagtttgaa cgcaacggcg acgacgataa acaccagacc cagtttcaac agccattcgt
    10441 ttgcttcttc attgaaggaa gaggcgagca cgccggcaaa ttccagcgaa ccggttgcgc
    10501 cgtaaaccat agaaataccg tagagcagca ggccggatgc cagcgcaccc agaacaaaat
    10561 atttcaaggc ggcttcggcg gcaaagccgg aatcgcggcg cagggcaatc agggcgtaaa
    10621 gggcaagcga caagagttcc aaaccgatat aggcagttaa aaaatgcccc gcgctcacca
    10681 tcacgcccat acccaagagg gcaaacagcg acagggtgta aaactcgcct ttgaaaatac
    10741 cgcgcacttg gttgtagggc ttggcataga caaacagggt aaacatcaag gcatataaaa
    10801 ccatttttgc caaacgcgac ataccgtctg caatatacat cccgttgaac gaagacgtgc
    10861 tgccctgttc ccacaccgcc aactgcacca cagccgtaac cgccaccgtt gccaacgcgc
    10921 cgtaatgcgt ccaccggcgt ttgtcatcac tgacccacaa gtccgccagc aacaataaca
    10981 ccagcagcgc aagcagcacg atttcgggca tggcgggcat taaattcaaa tcagaccagt
    11041 tcatttacac acctcaaatc ttgctttgtg ccacatgggc aatcaaatcg tttgccgcct
    11101 gatgcaccac ttcgataaat gcgttcggat acaggcccat acccaatacc gcaaccgcca
    11161 aaaccgccag aatcgtaaac tcgcgacagt tgatgtcttt catttcggca acgtgcggat
    11221 tgtgaatcgc gccgaaaatc acgcgcttgt acatccacag ggtataagac gcaccgtaaa
    11281 tcagcgtcat agccgccaac gcgcccaccc agaaattcac tttaaccgcg cccataatca
    11341 ccataaactc gcccacgaag ccagaagtcg ccggcagacc ggcattggcc ataccgaaca
    11401 acatcataaa cgccgcaaac ttaggcatca cattgaccac gccgccataa tcggcaatat
    11461 tgcgggtatg caggcggtcg tacatcacgc cgatacacat aaacatcgcg gcagacacaa
    11521 aaccgtggga aatcatttga atgattgcgc ctttcaatgc ccaatcattc aattggccgt
    11581 tcacaaacag gaacataccc aaagtgacaa aacccatatg gctgatggac gaatacgcca
    11641 ccagtttttt catatcggtt tgcaccaaag ccaccatacc gatgtaaatc acggcaatca
    11701 ggctcaatac aatgatcaca ggggcaaaat agcgtgccgc atccggcata atcggcagga
    11761 taaagcgcaa gaaaccatac gcacccagct tcagcgtaat cgccgccaac accatcgaac
    11821 cgccggtcgg cgcttcaacg tgggcatccg gcaaccaagt gtgcacaggg aacatcggca
    11881 cttttacggc aaatgacagg aagaacgctg caaataaaag ctgttgtacg cccaacggaa
    11941 tctgtttgat gttttggaaa tcgacaatag agaagctgcc tgtttgatag tacaggtaaa
    12001 ccatagcaac cagcatcagg agcgaaccca tcagcgtata gaggaacagc ttgaccgacg
    12061 catagacgcg gcgcgggccg ccccatacac cgataatcag gtacagcgga atcagcatac
    12121 cctcgaagaa cacataaaac agaatcgcat cctgcgcggc aaacgcaccg ttaatcaaac
    12181 ccgacatcat caggaatgcc gccatatact gcgccgggcg tttctgaata acttcccaac
    12241 ccgccagcac caccaacagc gtaataaacg cattcaagat gataaagagc actgaaatac
    12301 cgtccacgcc caatgcatag ttgattttca gcagcggaat ccactcgtgg aactcggtaa
    12361 attgatagcc gccgctcaaa cggtcgaaac cggtaaacag gggcagcgtt accaagaaac
    12421 cggcaagcgc acccataaag gcaagcacac gcgccagcgg cgcacggctg tccttaccgg
    12481 tcgacaaaac cagcacacct gcggcgatgg gtatccatat tgccaagctg agtaggtagt
    12541 tggaaaacat agtggttaac ctgtggttaa aataaaaata tcgttgtacg ggttggtttg
    12601 tcagacgacg ttttaagaga ccgtctgaaa cctcatcctt atctgaacaa tccccagaag
    12661 gtcataccga gcagtaccaa tacgccgaac accatagcgg cggcgtaggt atagataaag
    12721 ccggtttgag ctttacgcac ttgcgcggca atcgcgccga ccagtttggc agagccgttg
    12781 acgatgccgt tgtcgataat ggcggtatcg ccgactttcc agaagaaagt acccaatgcg
    12841 cgtgtgcctt tggcgaaaac gttgaaatac agggcgtcga ggtagtattt gttttcaaac
    12901 aaaacgtaaa tcggacggaa cgtctgtgca attttcgctg gcaggtgcgg cagtttgacg
    12961 tacaaaagcc atgcgctcaa cacgcctgcg atagcaaggt agagtacagg cgaatgcagg
    13021 ctgtgcgaca ccattgccaa tgcgccgtgg aactcttcct tcatgatgtg catggtcgga
    13081 tgcgcgtcgg cgttgacgaa aatcacatct ttgaagaaat cgccgtaaag cataggctcg
    13141 atggcaacgt agccgatgat gacggacgga atggcaagca aaatcaaagg cagggtaaca
    13201 accaacgggc tttcgtgcgg attgtcgttt ttgcccaagc tgtgatgttc ttcgccatga
    13261 tgatcgtcat gatgttcagg caagctgcgc catttctctt cgccgtggaa caccataaag
    13321 tattggcgga acgcgtagaa cgcggtaacg aacacgctgg caaggacggc aaaataggca
    13381 aagccgctgc ccggcagggt gctgtatttc gccgcttcga taatcgaatc tttagaatag
    13441 aagccggaga agaacggcgt accgatcagc gacaagttac cgatcagcat ggtcagccaa
    13501 gtaatcggca tgtatttttt caggttgccc atgtggcgca tgtcttggtc gtggtgcata
    13561 ccgataatcg cgctgcctgc cgccaagaac aacaaggctt taaagaaagc gtgggtcatc
    13621 acatggaaca tcgccacgga ataggcagac gcacccaaag ccacggtcat atagcccagt
    13681 tgcgacaagg tggaatacgc aaccacacgt ttgatgtcgt tttgaatcac gcccaagaaa
    13741 cccataaaca gggcggtaat cgcaccgatc accataatga cagacagcgc agtgctgctc
    13801 atctcataaa tcggcgacat acgcgatacc ataaacagac cggcggtaac catcgtcgca
    13861 gcgtgaatca atgcagaaat cggggtcggg ccttccatcg aatcaggcag ccaaacgtgc
    13921 agtgggaatt gtgccgattt acccatcgca ccgacaaaca ggagcaaaca ggtgacagta
    13981 atcaacgacc attccacacc ggggaaaagc tggatggtgg cattttgcac gttgggcaga
    14041 taagcgaata catcttgata gcgcaggctg ccgccgaaat aggcaagcac caagccgata
    14101 ccgagcaaaa agccgaagtc gccgacacgg ttgatcaaaa aggctttcag gttggcaaat
    14161 gttgcgcttg gacgtttgaa atagaaaccg atcaagagat acgacaccaa gcccacagcc
    14221 tcccagccga agaagagctg gatgaagttg ttgctcataa tcagcatcaa catgctgaaa
    14281 gtaaacaaag aaatatagct gaagaagcgt tggtagccga ctttttcatc gtgcatatag
    14341 ccgatggtgt agatatgcac catcaacgat acgcccgtta ccacgaccat catcatcgcc
    14401 gtcatcgtat cgaccaagaa gccgacggag aaatccaaac cgcccatcgt cagccaggta
    14461 tagacgttct catcaaactt ggcacggctg ccattgagga atccccacag cacataagcc
    14521 gacagcacgg cagacaccgc cacaccgagt atcgtaacag tatgcgcacc ggcacgaccg
    14581 attttgttgc cgaacaaacc cgcaatcaac gagcctgcca acggaacaag ggcaattatc
    14641 aaatataaag tcatgtcgtt catttgattg aatccgattg atttaaaaat ctatgtttgt
    14701 ttcgtacaaa attacttcgg aaaaacaaat ccaacacgct ccaatcgttt gcgtgccaca
    14761 gctaattgct cttcagtaaa taaatcacac cacggctttt gtaacaccag atattccata
    14821 ctgtatttca aggcgtggac tccgccacca ctcagaatca gctctgtaaa accggtctga
    14881 gtcttctttt cccccgtact taataattta tccgccgcct ctttaccacc aaattcattt
    14941 acaatttgta aaaatcgtgt cgccttgtaa ggttgcggca aattcaaagc ctcctgataa
    15001 atatttaaca tggctttatg aaattcttgt tctaactgat ttttatccat cattcttctt
    15061 ccaatatttc agaccggatt attcttaccc agaatttctt ttctcatccg ctcccgtctg
    15121 atcacctacc gaatcaggtc gtctgaaaca gtctgaaatc gcttttcaga cgaccctcag
    15181 cctttttcat acccttcgta ataatacgac tgctcgatac ctttaaagat gatttcacgg
    15241 ttgtccacat cgtcggtcag gttgtccttt aacagaaagc gcagttctaa atcgttgacg
    15301 gggctgcgtt ccatcgcctg caaatacagg gttttgctta cattttgcca gtttacgatt
    15361 tttttcaggt ttttcttcag caccaaatcc agccagatgc gggtacttct gccattaccc
    15421 tccaaaaacg gatgggcaat gttcatttca acatatttgg cgatgatttc ttcaaaagtc
    15481 cgctcgggca tctgctcgat tttaaccaaa gcctctttta aatacatggc gttggcaaaa
    15541 cgaaaaccgc ctttggaaat gttgtcttcc ctgatttgac ccgcaaaatc atataagccg
    15601 ccgaacaggt aacggtgaat ctgttgcagg cccgcggtgg taccgacttc gatacggtcg
    15661 atgtcgccgc tttcaaacag gcggcgggca ttatgcaggc tttgttcgtc tatggatttc
    15721 atcgtttttc ctatcgggtt ttcagacggc atcggtgtct tgtcaggtct cgacccaacc
    15781 tacctttacc ctttcaactc gtccaaatcg gcaacattaa tcgtttgtcg gttgcggtac
    15841 accaatacca taatcgccaa accgatggca gattcggcag cggcaacggt caatacgaag
    15901 aatacgaaaa tttgtccggc agtatcgccc aaatgttgcg agaaggcgat aaagttgaag
    15961 ttcaccgcca agagcatcag ctcgatggac atcagcaata ccagcacgtt tttgcggttc
    16021 ataaagatac ccattgcgct gataccgaac aggagcgcac ccaataccag ataatgcgtc
    16081 aaggtaatca tgctttgccc tccccttccg gcttgaggcc gtctgaaact tcgctctctt
    16141 cggcagattc gacttgcggt ttgaccgctt ccattttcac cagacgcata cggccttgat
    16201 cggcgcgtac tttaacttgg tcggcaggat ccatgcgttt cggattggtg gttttacggt
    16261 aaaccagcgc aatcgccgcc accataccca acagcagcaa taccgccgcc aattcaaacg
    16321 gcaacaggta gtcggtataa atacggctgc ccaaatcgcg gatattgttg taatcggccg
    16381 gaatgtcttt catcagacca aatgcggcca ggtcggtttt cgggttgacc agaatcagga
    16441 tcagggcaac cgccaacaat gtgccgacca caccggcaac cggcgcatga cgccagaaac
    16501 cggcgcgcat ttcttcaatg tcgatgttca acatcatcac aacgaacagg aacaacacca
    16561 tcacggcgcc gacgtaaacc accaccagcg tcacgcccaa aaactcggcc tgcatcagca
    16621 tccaaagcat cgcgctcacg cagaaggtca gcaccagatg caaagcggcg tggacagggt
    16681 ttttggcggt aacggtacga accgcaccgt acaaaacgat ggcggcaaga atatagaaca
    16741 gaatcgcgga aaaagtcatt cgtctgctcc ttaacgatac ggcgcgtcag cggctttgcg
    16801 tttggcgatt tcagcttcgt atttgtcgcc aatggccaaa agaatcggct tggtcatgtg
    16861 caagtcgcct tttttctcgc cgtggtattc aaaaatatgg gtttccacaa tcgcatcggt
    16921 cgggcaggct tcttcgcaga aaccgcagaa gatgcacttg gtcaggtcga tgtcgtaacg
    16981 cttggtgcgg cgcgtgccgt cttcgcgttc ttccgattcg atgttgatcg ccattgcggg
    17041 gcaaacggct tcacacaatt tacacgcgat gcagcgttcc tcgccgttcg gataacggcg
    17101 ttgcgcgtgc agaccgcgga aacgcacgga ttgcggcgtt ttctcttcgg gaaaataaat
    17161 tgtgtctttg cgggcaaaaa agtttttgag cgttacgccc atgcctttta ccaactcgcc
    17221 gagcagaaag gtttttacta agttagccat attatgttcc ctcaaaacag ggatttcgtt
    17281 aggtattcaa aatcgctttg ttcagacggc ctcaagatgc cgtctgaaac ttatttccac
    17341 aaattcagcg gtgaaatcat ccacacgccc aaaatcacga tgtaggcgaa gccgatcgga
    17401 atcagcactt tccagcccaa acgcatgatt tggtcgtaac ggtagcgcgg gaaggttgca
    17461 cggatccaca gataccagta caacactgcc gccattttca caaacatcca gaatgcggaa
    17521 ggcgtaccga taaagcccca gctttgcggg aacggagaca accagccgcc gagaaacatc
    17581 agcgatgtca gcgcggcaat cagaatcatg aaaatgtatt cggcaaggaa gaacagcgcg
    17641 aatgcgaagc cggaatattc aacgtggtgt ccggcaacga tttcggactc gccctctgcc
    17701 acgtcaaacg gtgcgcggtt ggtttcggca acggcggaaa tcagatagac gatgaagatt
    17761 gggaacagcg gcagccagtt ccacgagaat acggaaccgc ccgcgatacc ttttgcctgc
    17821 gcggcaacga tgtcggagaa gttcatgctg cccgacacca tcacgacaca caccagcgcg
    17881 gcactcatgg caatctcgta ggaaatgctt tgtgcggaag cacgcattgc acccaagaac
    17941 gaatatttgg agttggaagc ccagcccgcg atgatcacgc cgtaaaccga cagcgaggta
    18001 atcatcagga tgtacaaaag accgatattg atgttggtca gcacccattc ttcgttgaac
    18061 ggaatcactg cccacgccgc gaaagacggt gcaagcgaca taatcgggcc gatgtagaac
    18121 agggctttgt ttgacagctt cggacgggtt acctctttaa acaagagttt gaacacatcg
    18181 gcaaacggct ggatcaggcc ccacgggccg gttacgttcg gaccgacgcg aagctgcata
    18241 aagccgatga ctttacgttc gaaataagtc aggtaggcga cggtcagaat cagcggaatc
    18301 aggataatca caattttgac gatgacggat accaccaagc ctacagtaat acccaaatcg
    18361 cccagaccga gcgttgcggc aaagaggttt tggaaccatt cctgcataat caagctcccg
    18421 ccagttcaat agtgtccatc aacgcaccca gcgcggcatt ttcggtatgc agcggcagat
    18481 gcaccacgtt ttcaggcagt ccggcatcgg ctttgacggc aaccgatacg cttgcgccgt
    18541 tttgtttggc gacagcggtt tgtccgtctt gcaggcccaa gcgtgccaat gtatttggat
    18601 ttacacgcgc agcaggcacg gcggcatggc tggtttcttg caacggtgcg gaacggcgca
    18661 cgatagaatc ggtgtgataa ataccgacgc cgccgacacg gacgaggcgg tctgaggtcg
    18721 tctgaacgcc ctcccctgtc catgcgttgc ggttgtccag tttggacggc aggctttcca
    18781 catccagcgc gtctttcaaa atcgcagcgg tatcgtggta ttcaaaacct ttcaggtcaa
    18841 acaggttgcc caatacgcgc aacactttcc acagcggacg cgaatcgccg aagccttgta
    18901 ccacgccgtg gaaggattgc agacggcctt ccatattgat gaagctgcct gaggtttcgg
    18961 taaacggtgc aatcggcaac aacacgtcgc acacgtccag cagcgtttcg ctgacaaacg
    19021 gcgtaaacgc catcacgctt tttgcctgtt tcaacgcggc tacggctttt gcaccgtccg
    19081 ccgtatcgat ttcaggctca acgttgagca gcaagactgc ctgtttcggc gcgtttacca
    19141 tttcgacaac gctcttgccg gagtttacat tcaagacatc cgcaccaacg ctgttggcgg
    19201 cttgcggcaa aatgcccagc actgcgccgg tcgcgtcagc cagctcttgc gcggcggcgt
    19261 aaaccgcggc gtaatcagga tggttttgca cttccgcgcc caaaatcacc gctgcttttt
    19321 cagcattttt caggctggcg gtaacggcgt gttccgcatt gacagacagg tttttcagac
    19381 ggcctgccca ctcgtcggga tgtgcggctt cttgagacag aagcggcata aacaattctt
    19441 ctttactgct ggccaatacg ctcaatgcca tacggtcttt ggcggcgcgg cgcaggcggg
    19501 cggtcaggag cggctgttct ttgcgcaagt tcgcaccgac taccaatacg gcatcgttgt
    19561 cagccaaaga ttcaatgctt tgtcccaacc attgcgcacc tttaaggccg tctgaaagac
    19621 gtttgtcttg ttggcgcaaa cgggttgcaa agtttttaac acccaagccg tcggcgagtt
    19681 tcttcgccag atacagttct tcaaccgtat tcatcgggtt cgcccaaacg ccgacttggt
    19741 tttgcttgcc gtctttggcg atacattcaa tcgcgctgcg gacatattcc aacgcggttt
    19801 tccaatccac gtccatccac tcgccgccct gtttgatttt cgggtttttc agacggcttt
    19861 cgtgatacag gccttcgtag gcgaaacggt cgcggtcaga cagccagcat tcgttaatcg
    19921 cttcgttttc caacggcaac acgcggcgga cggtatggtc tttggtctgc acaatcaggt
    19981 tgctgcccaa agcatcgtgg gcggaaacgg atttgcggcg gttcaattcc caagtacgcg
    20041 cgttgaagcg gaacggtttg ctggtcagcg cgccgacggg acacaaatca atgacgttgc
    20101 ccgacaattc ggtttccacc gttttgccga taaagggcat gatttcggag tgttcgccgc
    20161 gattcaccat cgcaatttcc tgcaaaccgg cgatttcttc agtgaaacga acgcagcggg
    20221 tgcagtggat acagcggctc atttcctcgg cggaaaccaa ggaccccata tctttgccga
    20281 cgacggaacg tttttcttcg gtgtagcggc tggtggtttt gccgtagccc accgccaaat
    20341 cctgcaactg gcattcgccg ccttggtcgc aggtcggaca atcaagcgga tggttgatga
    20401 gcaggaactc catcacgcct tcctgcgcct ctcgggcttt tgccgaatgc gtacgcacaa
    20461 tcatgccgtc tgtaaccggc gtggcacagg caggcagggg ttttggggct ttttctacgt
    20521 tcaccagaca catacggcag ttggcggcga tggaaagttt tttgtggtaa cagaaatgcg
    20581 gaatataagt accgagcttg tgcgcggctt caatcaccgt cgcgccctgc tccacagata
    20641 cttgtttgcc gtcgatttcg atttgtaaca tggttcgttc ctagttacgg gtatttgata
    20701 aataatcttt tatggaggtc gtctgaaaaa tgggtttcag acggcctttt gagtttcatt
    20761 gacaatcaat actgtttaaa acgtttactg ccaccttgcc gtcattcccg cacaggcgtg
    20821 aatccattct tgggttttcg gtaactgttt tcaaacattg gtttcttaac ttttgcggtg
    20881 attcccgccg tagcctgtct gcgcgtaggt ggggctggaa taacgacagt ttttaaagtt
    20941 ccagaaataa aactaaacaa acaacacgcc cgacaagtcc aatcattcag tattttgttc
    21001 ttcaatcagt ttaatcttcc attcacgtcg ccattttttc atcgtttttt ctttggcaac
    21061 tgcttcaggc atattctcaa aaaactgata ccaaactaaa tcatgcacat catatcgggc
    21121 agtaaagcca tcaatattga catggttttt gtgctgccaa accctttccg gcaaattcat
    21181 ggtaacaccg atatagagtg ttccattttt accgtttgcc aaaatatata cggcaggttg
    21241 tctagtaagc ggatggtttc gtattcattt ttcctgcaat ataaacaaca acttttttaa
    21301 tatcatttac aaccgttttc atctgaaaac atctgcctac tcccgccgtc attcccgcgt
    21361 aggcgggaat ccattttttg aatttcggca actgcttttc aaatatcggg ttctgtaaat
    21421 tccactatgg attcccgcct acgcgggaat gacggcaaag ttaaattttt agcattttgc
    21481 ttttaaccaa tataaaacca actaaaacta taactcattt attatatttt taaatgactt
    21541 aaataactct ttaacagcac gctcattgtc ttcatctgta ggctcaaact tttcacacca
    21601 attaattata tttacatcgc catgcaataa ctctgcataa catcctactg catcgcaact
    21661 accaccccgt gtccttgacg tgccaaaaat aaaatcacaa ttatgcgaat aaagtttgta
    21721 taaatttccc ctaacctcgt attcattatc accagatgaa taaaaaccaa ttctctgacc
    21781 ttcatgatca aatactgcaa caaaatctaa gccgtttcca tgtctttcaa aaaagactag
    21841 aaatttagaa aatttccgac aaatctgatt aaaaagcgta ttgagtgtcg tactcttacc
    21901 tttgttggct gcaccgtata gaataaaaat tttagcttcc accattacca ccctccccac
    21961 ttatgctctt tcatcggccc gccgtgttcg atgtaatgca caaactcatc acggaaatgc
    22021 ttggtaaagc tgcggacggg gaagacggca gcatcggcga gggcgcagat ggtgcggcct
    22081 gccatttggt tgccgacgga atccagcaaa tccaaatctt ccattttacc tttgccttct
    22141 acgatgcggt ggacgatgcg gtaaagccag cccgtacctt ctcggcaggg ggtacattgg
    22201 ccgcaagact cgtcgtagta gaagtagctc aaacgctcaa gggctttgac catgcacacg
    22261 tcttcgtcca tgacgataat cgcgccggaa ccgagcatgg agcctgcttt ggagatcgag
    22321 tcgtagtcca tattggtctg catcatgatg tcggcaggca atacgggcgc ggacgaaccg
    22381 ccgggaatga cggctttgag ttttttaccg ccgcgcatac cgcccgccat tttcaagact
    22441 tcggcaaacg gcgtacccaa tggcacttca tagttgcccg gacgctcgac atggccggaa
    22501 atacagaata atttggtacc gcctgcattc ggaatacctt tatcggcaaa tgcctgtcca
    22561 ccgtcacgga taatgaatgg aacggaggag aacgtttcag tattgttgat ggtagtcggt
    22621 ttgccgtaca ggccgaacga agcagggaat ggcggcttaa agcgcggctg gccttttttg
    22681 ccttccagcg attcgagcaa tgcggtttcc tcgccgcaaa tatatgcgcc gtagccgtgg
    22741 tgggcgaaga gttcaaattc aaaatccgaa cccaaaatat ttttacccaa aaagcctgcg
    22801 gcacgcgcct gctccaaagc ggcctcaaag cgttggtagc cttcaaaaat ttcgccgtgg
    22861 atatagttgt aaccggcttt cgcgcccatc gcgtaaccgg cgataatcat gccttcgatc
    22921 agggcatgcg gattgaacat gatgatgtcg cggtctttaa acgtacctgg ttcgccttcg
    22981 tcggtgttgc aaaccacata tttttcgccc gggaaagaac ggggcataaa gctccatttc
    23041 aaaccggtcg ggaagcccgc accgccgcgc ccgcgcaaac cggaggtttt gacttcgtca
    23101 atcacatcgg tttgcgagat gttttcggac agaattttac gcagggcggt atagccgccg
    23161 cgtttgacgt attcgtccaa tgtccagcaa tcgggattgg cggtatccac ttggtcaaaa
    23221 atcacgcctg attggtaaat agccattttt ggtgtgcctg tttgttttcg tatcggttgc
    23281 ggccgctgtt tcagacgacc ttaagatgtc tttgtgtacc ggcttgtaac gtcgtctgaa
    23341 ataaaatcta gtttatcaaa tcgatatttt cagacgacct ttaatcgttt ttgttggatc
    23401 tcgaccactt gcttgtctgt cgtcattccc gcgcaggcgg gaatccatcc tcaatggtaa
    23461 gcaatgtctt attaaattca gaaaccgaat cttaccggtg gattcccgcc tgcgcgggaa
    23521 tgacggcatt tcggtatttc agtagggcgg attcttaaat ccgacatttt gcctttttac
    23581 ccactctgtc ggctacaagt atccgaccta cgtttaaatc gtcgtttcag acgacctact
    23641 ccaactccgc cagtttcttc tcaatcgctt cttcggtcat aaagctgcac atgctgtggt
    23701 tgttgaccag cataacggga gcgtcgccgc atgcgcccat gcattcgcct tcgacaaggg
    23761 taaacttgcc gtcaggggta gtttcgccgt agccgatacc gagtttttgt ttgaggtatt
    23821 cgccggtagc cataccgccg cgcagggcgc agggcaggtt ggtacaaacg gtcagtttgt
    23881 atttgccgac aggctcaagg tcgtacatat tgtagaaagt ggcgacttcg taggcttgtg
    23941 caggcgtgat gccgatgtag tcggcgacaa aagcgatggt ctcgggagca agccagcctt
    24001 tttcggtttg ggcaatacgc aatgcgccca taatcgcgga gcggcgttgg tcggcaggat
    24061 attttgccaa ctcgatgtcg atttgtttta aagattctgc ggataacatt atcggtcaac
    24121 ctccccgaat acgatgtcct gcgtaccgat gatggcaacg acgtcggcga gcatgtggcc
    24181 ttttgccatt tcgtccatgc cttgcagatg ggcgaagccg ggtgcgcgga ttttcaggcg
    24241 gtagggtttg tttgcgccgt ctgaaatgat gtaaacgccg aactcgcctt tcggatgttc
    24301 gacagcggtg taggtctcgc cctcgggaac gtgcataccc tcggtaaaga gtttgaaatg
    24361 gtgaatcagg tcttccatac ctgttttcat ttcggtacgt ttgggcggag cgaatttgtg
    24421 gtttgtggta atgaccggac ccggattgac acgcaaccac tcggaacatt gtttgatgat
    24481 gcgtacggat tgacgcattt cttccatacg gcagaggtag cggtcgtagc agtcgccgtt
    24541 cacaccgaca gggatgtcga aatccatttt gtcgtacact tcgtaaggct gtgtcttacg
    24601 cacgtcccat tccacgcccg aaccgcgcaa catcacgccg gtaaagcctt tttgcatggc
    24661 acgttcgggg gagacgacgc cgatgccgac ggtacgctgt ttccaaatac ggttgtcggt
    24721 caggagggtt tcgagtgtgt cgatattttt ggggaagcgt tcgcagaagg catcgataaa
    24781 gtcgagcatg gtgccttcgc gggattcgtt gagctgcttc aatactttgg cattgcggaa
    24841 tttgctgccc tcgtatttgg gcataaagtc gggcaggtcg cggtaaacgc cgccgggacg
    24901 gaagtaggcg gcgtgcatac gcgcgccgga cacggcttcg tacaagtcca tcagctcttc
    24961 gcggtcgcgg aaggcgtaaa gaatggcggt catcgcgccg atgtcgaagg catgcgaacc
    25021 gatgcccatc aagtgattga ggatgcgcgt tacttcggca aacatcacgc ggatgtattg
    25081 ggcgcggatg ggcacatcga taccgacaag tttttctact gccaaacaat acgcctgctc
    25141 attgaccatc atggaaacat agtccaagcg gtccatatag ggcagggctt gcagataggt
    25201 tttggtttcc gccagttttt cggtacctcg gtgcaagagg ccgatatgcg ggtcggcacg
    25261 gacgatttgt tcgccgtcca gctccaaaat catacgcaat acgccgtgcg ccgcagggtg
    25321 ttgcgggccg aagttgatgg tgtagtttct taatttattg gccaccgtag ttctcctcac
    25381 ggacgatacg cggcgtgatc tcgcgcggct caatggtaac aggttggtaa atcacgcgtt
    25441 tttgctcttc gtcgtaacgc atttccacat agccggaaat cgggaagtct ttgcggaacg
    25501 gatgtccgac gaagccgtaa tcggtcagga tgcggcgcaa gtccggatgg ttgttgaaca
    25561 tgatgccgta catatcgaag gcttcgcgtt cgtaccaatc cgcgctgttg taaatatcga
    25621 ctacagattc gactacgggg aagtcgtcgt ctgaaaccca gacgcgcacg cggatgcgtt
    25681 gattgttttt aacggaaagc aactgactga cgacggcaaa gcgtttgccc tgccatgctt
    25741 cgtttttgta agtgctgtaa tcgacaccgc acaagtcaac cagaagctcg aaatgcaact
    25801 cttcatggtc acgcaatgcg gtcatgactg aaatatagtg ctcgggcaga cactcgacgg
    25861 taatctcgcc caaagcggaa atgactttgc ctgcctgatt gcccaaaacg cggctgacgg
    25921 tttcgtataa gtcttgaatg cttgccatat cgtcctctcc ttactcgtca cgcgcaatgg
    25981 tggaagtgcg cttgattttt tgttggagct gaatcaggcc gtaaatcagg gcttccgcag
    26041 tcggcggaca acccggcaca taaacatcta ccggcacgac gcggtcggca ccgcgcacaa
    26101 cggaataaga atagtgataa tagccgccgc cgttggcaca tgagcccata gacaataccc
    26161 agcgcggctc ggcgagctgg tcgtacactc ggcgcagggc gggcgccatt ttattggtga
    26221 gcgtacccgc cacaatcatc aggtcggcct gacgggggga cggacggaaa ataataccga
    26281 aacggtcaag gtcgtaacgc gccatacccg cgtgcatcat ttccacggcg cagcaggcca
    26341 agccgaaagt aaccggccac aacgaaccgg tacgcatata gttcagcacc gtatccgcgc
    26401 tggtggtgat gaaacctttt ttcaaaacgc cttctattcc cattccagcg cacctttttt
    26461 ccattcgtaa acaaagccta ccgtcagaac aacgataaac accagcatag accagaagcc
    26521 gtacgcgccc aaatctttga acacgactgc ccacggcagc ataaacgcga cctccaaatc
    26581 aaacaggatg aagaggatgg cgacgaggta atagcgcacg tcgaacttca tcctggcgtt
    26641 ttcaaaagct tcaaaaccgc attcgtaagg cgcgtctttt tcggcatagt ggcgtttcgg
    26701 gcctaaaatc gtgccgagca ggataaacag cacgccggcc gcgaggccga tgaggataaa
    26761 gacaaagacg ggaaagtaag cggacaacat ggttacaccc aaatccgtta acaaaatttc
    26821 tacaataatt tcgtatttta gcgaatttca aaaaccatta aaaggtaaat atcggcaaaa
    26881 cgcccaaaaa aacccaataa atacaatcat gttatgataa cgattcttat ttgattttat
    26941 aaggtcatat aaatttttac accgtaattc g
//
LOCUS       DAWGRZ010000007        26438 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000007 DAWGRZ010000000
VERSION     DAWGRZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26438
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.007"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            693..2141
                     /gene="gnd"
                     /locus_tag="WHT44_000214"
     CDS             693..2141
                     /gene="gnd"
                     /locus_tag="WHT44_000214"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ2532455.1"
                     /translation="MNGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTTAKVDEFLNG
                     AAKGTNIIGAYSLQDLVDKLEKPRKIMMMVRAGSVVDEFIEQLLPLLEEGDILIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARRGPSIMPGGDKRAWDAVKPIFQAIA
                     AKTSQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMYRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2204..3475
                     /gene="waaA"
                     /locus_tag="WHT44_000215"
     CDS             2204..3475
                     /gene="waaA"
                     /locus_tag="WHT44_000215"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ2532456.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQSLIRELRRRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMRECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDIIPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKTLSSEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGYRGR"
     gene            3516..3965
                     /locus_tag="WHT44_000216"
     CDS             3516..3965
                     /locus_tag="WHT44_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ2532457.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSREARRE
                     VEKAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            3998..4969
                     /locus_tag="WHT44_000217"
     CDS             3998..4969
                     /locus_tag="WHT44_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ2532458.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNPGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEKEDLANSRLLNTVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASVLTSLVYTKKTGR"
     gene            5043..5118
                     /locus_tag="WHT44_000218"
     tRNA            5043..5118
                     /locus_tag="WHT44_000218"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5076..5078,aa:Lys,seq:ttt)
     gene            5489..6742
                     /gene="murA"
                     /locus_tag="WHT44_000219"
     CDS             5489..6742
                     /gene="murA"
                     /locus_tag="WHT44_000219"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ2532459.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELQGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVEQLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            6823..8034
                     /locus_tag="WHT44_000220"
     CDS             6823..8034
                     /locus_tag="WHT44_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ2532460.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     KYCVDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGSLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIAADAEAVVKDIADVAEDEMILDIGPKSAAALADLLKAAGTVVWNGPVGVFEFDQ
                     FAGGTKALAEAIAQSKAFSIAGGGDTLAAIAKFGVTEQIGYISTGGGAFLEFLEGKEL
                     PAVAALEKRGA"
     gene            complement(8101..8349)
                     /locus_tag="WHT44_000221"
     CDS             complement(8101..8349)
                     /locus_tag="WHT44_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ2532461.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8440..9357)
                     /gene="ftsX"
                     /locus_tag="WHT44_000222"
     CDS             complement(8440..9357)
                     /gene="ftsX"
                     /locus_tag="WHT44_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ2532462.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLMMLAVAMTLPLF
                     MHLGIQSGQSVLGKLNESPQITIYMETSAAQSDSDTVRSLLARDKRLDNIRFIGKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYAGELG
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(9354..10004)
                     /gene="ftsE"
                     /locus_tag="WHT44_000223"
     CDS             complement(9354..10004)
                     /gene="ftsE"
                     /locus_tag="WHT44_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ2532463.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTI
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(10198..10680)
                     /locus_tag="WHT44_000224"
     CDS             complement(10198..10680)
                     /locus_tag="WHT44_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ2532464.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGEVNEKSLTDAVKLAHSKCR
                     "
     gene            10804..11157
                     /gene="arsC"
                     /locus_tag="WHT44_000225"
     CDS             10804..11157
                     /gene="arsC"
                     /locus_tag="WHT44_000225"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ2532465.1"
                     /translation="MPEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPNLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            11154..11798
                     /locus_tag="WHT44_000226"
     CDS             11154..11798
                     /locus_tag="WHT44_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ2532466.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGRDFFN"
     gene            12003..13397
                     /locus_tag="WHT44_000227"
     CDS             12003..13397
                     /locus_tag="WHT44_000227"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ2532467.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLNYDNADNVVYQTRRFDRYKEVIAELLEKGHAYYCYC
                     SKEELEAMREKAEKEGSATYDRRWRPEVGKTLPEIPSDVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIDANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLELVKDRPQDLNTLADECFYFYVKQTPAEADVQKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            13418..13813
                     /locus_tag="WHT44_000228"
     CDS             13418..13813
                     /locus_tag="WHT44_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532468.1"
                     /translation="MPSDVGIRLQTAFKWKLKMKKIFYFLMVVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQTAQADIARLEAEIPAAMAQKARQ
                     AEDLRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            13817..14308
                     /locus_tag="WHT44_000229"
     CDS             13817..14308
                     /locus_tag="WHT44_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ2532469.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDDELPLAFAAVDDSGILLGSAAVKRHDMESFPRYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWVVVGRHFHNGEWVTVMRLDV
                     DKV"
     gene            complement(14432..17026)
                     /gene="mutS"
                     /locus_tag="WHT44_000230"
     CDS             complement(14432..17026)
                     /gene="mutS"
                     /locus_tag="WHT44_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ2532470.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKSIADIERIAARIAVGNARPRDLAALRDSLFALSEIELSAECSSLLGTLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHTAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17470..18474
                     /gene="gap"
                     /locus_tag="WHT44_000231"
     CDS             17470..18474
                     /gene="gap"
                     /locus_tag="WHT44_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ2532471.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVASGSLTELVSILERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18544..18663)
                     /locus_tag="WHT44_000232"
                     /pseudo
     CDS             complement(18544..18663)
                     /locus_tag="WHT44_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18732..19412)
                     /gene="tsaA"
                     /locus_tag="WHT44_000233"
     CDS             complement(18732..19412)
                     /gene="tsaA"
                     /locus_tag="WHT44_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ2532472.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAKACIELNPKFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            19526..20161
                     /locus_tag="WHT44_000234"
     CDS             19526..20161
                     /locus_tag="WHT44_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ2532473.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ"
     gene            20198..21166
                     /gene="waaC"
                     /locus_tag="WHT44_000235"
     CDS             20198..21166
                     /gene="waaC"
                     /locus_tag="WHT44_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ2532474.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFRAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKMAKSPICGLDKNSAREGWAALAYVKTYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            21393..22142
                     /locus_tag="WHT44_000236"
     CDS             21393..22142
                     /locus_tag="WHT44_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ2532475.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLTPDDLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            22153..23088
                     /locus_tag="WHT44_000237"
     CDS             22153..23088
                     /locus_tag="WHT44_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ2532476.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKVDLLVAGNGASAVV
                     EFAKQVAGVKKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRATVFDAVAAQGGN
                     AEVINVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEVLKN"
     gene            23151..23762
                     /locus_tag="WHT44_000238"
     CDS             23151..23762
                     /locus_tag="WHT44_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ2532477.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     ILERDLQLDCRLTVSLSLVNYEACVAAWKNTRQIEVNTEKPFE"
     gene            complement(23814..24131)
                     /locus_tag="WHT44_000239"
     CDS             complement(23814..24131)
                     /locus_tag="WHT44_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ2532478.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKHVVTLIRDMDNFYT
                     HEYQACHQAIEDGVELDLSTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            24289..25560
                     /gene="purD"
                     /locus_tag="WHT44_000240"
     CDS             24289..25560
                     /gene="purD"
                     /locus_tag="WHT44_000240"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ2532479.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGIVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDIISGLDAANQIGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            25594..26196
                     /locus_tag="WHT44_000241"
     CDS             25594..26196
                     /locus_tag="WHT44_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ2532480.1"
                     /translation="MTNVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
BASE COUNT         6351 a         6090 c         7438 g         6559 t
ORIGIN      
        1 gagcggtttc tgttgctccc gataaattcc taaaactcaa aatttcatca ttcccacttt
       61 cgtgggaatg acgcggtaca ggtttccgtt acgtatcccc atccgaaagt ttacttatac
      121 acaagcaatc caaagttatc tgactgaaat attgtgagat ttacccgcaa atctcatttt
      181 ttccaccact tatacacaaa ttcggatgcg cactctgccg atatgcagaa ataagacatt
      241 cgtgccgaca cctgaccatt tccacaagtt ccaaacacac tttcctctca aacctccaca
      301 gtattccggt tcttaaagac atcggcagcc attcggtaaa gaaaagtagg atgccatttg
      361 cacaacaagc aaactgtcca caaaaactat tcttcatgaa aaacaatcca caggtatgca
      421 caaaagggaa tccgccttac cgacacccta aaaaaaagaa atcctgatga tttttaaaat
      481 aaaaaaaagt tacccacaga aaatttctcc tatcaatata atcatcgttt tattatttaa
      541 attttatatt ttatttataa cccttcagca aaacggtaca gcaaagcgta aaggcatctt
      601 ttcaaaaaat taaaaagtta aggattgtta aacaagtgta aatcgggtag agttgcggtc
      661 gataaattaa atctataacg gagcttcctg caatgaacgg cgatattggt gtaatcggtt
      721 tggcggttat ggggcaaaac ctgattttga atatgaacga ttgcggattt aaggtggttg
      781 cctacaaccg cacgactgcc aaggtggacg aatttttaaa cggcgcggca aaaggaacta
      841 atattatcgg cgcgtattct ttgcaagatt tggtcgataa gctggaaaaa ccgcgaaaaa
      901 ttatgatgat ggttcgtgcc ggttcggtgg ttgacgaatt tatcgaacaa ctgcttccgc
      961 tgttggaaga aggcgacatc cttatcgacg gcggcaatgc caattacccc gacacgacac
     1021 ggcggacaca ttaccttgct gaaaaaggga ttttgtttgt cggcgcgggc gtgtccggcg
     1081 gggaagaagg ggcgcggcgc gggccgtcca ttatgccggg cggggataag cgcgcttggg
     1141 atgcggtcaa gccgattttt caggcgattg ccgccaaaac gtcgcagggc gaaccgtgtt
     1201 gcgactgggt cggcaaagac ggtgcggggc attttgtcaa aatggtgcat aacggcatcg
     1261 aatatggcga tatgcagttg atttgcgaag cgtaccagtt catgaaagac ggcttggggc
     1321 tgtcttacga cgaaatgtac cgcgtgtttg ccgagtggaa caaaaccgag ctggattctt
     1381 atctgattga aatcacagcg gcaattttgg gctataagga cgaaggcggc gaaccgctgg
     1441 tcgagaaaat cctcgatacg gcagggcaaa aaggtacagg caaatggacg ggcatcaatg
     1501 ctttggattt gggtattccg ctgacgctga tttccgaagc cgtattcgcc cgctgcgttt
     1561 cgtcgttcaa agaacagcgc gtgcagaccg gcaaactgtt cgcccgaacc gttacgcccg
     1621 tcgaaggcgg caaacaagaa tgggtcgagg cgttgagaca ggccctgctt gcatctaaaa
     1681 tcatttccta cgcacaaggc tttatgctga tccgcgaagc gggcgaaagc tacggctggg
     1741 atttggacta cggcaacact gcgctgctgt ggcgcgaggg gtgcatcatt cgcagcgcat
     1801 tcttaagcaa tatccgcgac gcgtatgaaa acaatcccga tttggtgttc ttgggtgcgg
     1861 atccgtattt caaaaatatt ttggaaaact gcctgccggc gtggcgcaag gtggttgcca
     1921 aggcggtcga atgcggcatc cctatgccct gcatggcttc ggcgattacg ttcttggacg
     1981 gctacacgac cgaacgtctg cctgccaacc tcttgcaggc acagcgcgac tacttcggcg
     2041 cgcacactta cgagcgcacc gacaaaccgc gcggtgagtt tttccatacc aactggacgg
     2101 gcaagggcgg agataccgct tcgacaacct acgatatttg attccgttgc cgaaatgccg
     2161 tctgaaccgt tcagacggca tttgttttgc aggaaataga aagatgttcc aatggcttta
     2221 tgatgtattg tggctgcttg cgccgatatg gatacggcgt tatttggaca aacgctccgg
     2281 aagtgccccg gcatatcggg cgcatcggga cgagcgtttc ggcaagccgt atccgaatcc
     2341 cgttaccggc gcggtttgga tacacgccgt ttcggtcgga gaaacgcgtg ccgcccagtc
     2401 cttgatacgc gagttgcggc ggcgttttcc cgatgcgccg ctgctgatga cgcagatgac
     2461 cccgacgggg cgggaaaccg cgcaagttct gtttcccgat gcgcaatgcc gctatcttcc
     2521 gtatgacaaa aaaacgtggg tacggcagtt tttgcgcgaa caccgcccga tgttcggcat
     2581 tttgatggaa accgaaatct ggcccaacct gatgagggaa tgccggcgcg cgggtgtgcc
     2641 gctgtttttg gcgaatgcgc ggctgtcgga aaaatcgttg aacggttatc tgaaagtccg
     2701 ccgcctgatc cgtcctgccg ccgcttcgct gacggggtgt ctggcgcaga cagaggcgga
     2761 tgcggcgcgg ttggcgaaat tgggcgcggc atccgtgcag gtgtgcggca ataccaaata
     2821 cgacatcata ccgtcggaac agatgaaaac gctggcgggg cagtttgaaa aacgcatcgg
     2881 aggccggccg gttgccgtgt gcggcagcac gcgcgtttat cggggtgaag acgaggcgga
     2941 aaaactgctg gcggcgtggc aacaatatcg cggcgatgcg ctgctggtcg tcgtgccgcg
     3001 ccatcccgag cattttcaga cggtatttga aacggcaaaa cgcttcgggt ttaaggttca
     3061 gcggcgcagc gacggtttgc cggtcgaacc tgatacgcag gtgtggatag gcgacagtat
     3121 gggcgagctg tatgcgtatt acctgtgcgc cgatgtcgct tttgtcggcg gcagtctggt
     3181 cgattcgggt tgtcagaaca tcatcgaacc gctttcctgc ggcgttccga cgatattcgg
     3241 cttttcaacc tacaattttt ccgaagcctg ccgacacgcc ttggcatcgg gtgcggcggt
     3301 tcaagtcgaa tcggcggatg cgtggcggga agccgttgaa aaaaccttat cgtccgaggg
     3361 gggggggatg cagatgcagg cgcgcgtgga cggctttatc gcacaacatc gcggagcggg
     3421 cgcgagaatc gccgaggcgg tgcgggaagc ggtatgcgga tatcgggggc gatagtgata
     3481 caatccgtat ccgagttttc cgtttggagc atcgtatgag tatttatgcc gtcgcgcaca
     3541 tcgttcatct gtattgcgct attgcctttg tcggcggcgt gttttttgaa gtgctggttt
     3601 tgtccgtcct gcatacggga cgggtgtcgc gcgaggcgcg gcgcgaagtg gaaaaggcaa
     3661 tgtcttaccg cgccgtcagg gtgatgccgt ttgtggtcgg actgctgttc gccagcggca
     3721 tcgtgatggc ggcaaaccgc tatctttcta tattgggcga accgtttgcc acttccttcg
     3781 gtacgatgct gacgctgaaa atcctgttgg cgttcagcgt attggcgcac ttcgccatcg
     3841 ccgtcgtcaa aatggcgcgt tccacactga cggtcggttg gtcgaaatac atacacgccg
     3901 tcgtctttac ccatatgctg ctgattgtct ttttggcaaa agcgatgttt tatatcagct
     3961 ggtaacacaa ttacatttca aatcaggaga ttgaaccatg acagaatatc ccggcatcgg
     4021 gtcgccgttg ttttacggcg ttttttttgc ggcagtgctg gtcatgattg ccttggatat
     4081 gttttcgctg aagaaaaacg gcagccataa agtcggcgtc aaagaagcct tggcatggag
     4141 cggcttgtgg gttgccgtat cctgcctgtt cgcgggctgg ctgtattttg aactcgccgg
     4201 caatcccggc tacggcgcgg cagtcgcaaa agaaaaagta ttggaattct ttaccggcta
     4261 cattttggaa aaatcgttgg cagtcgacaa tattttcgtg ttcctgatga tattcggcta
     4321 cttcaaagtc gcaccacagt ttcagcaccg cgtgctgctg tacggcgtat tgggcgcatt
     4381 ggtattgcgc accgtcatga ttttcgtcgg cgcggcactg gttcagcagt ttgagtggat
     4441 tctgtatctg tttggcgcgt tcctgctcta tactggtata cacatgatga agcccgaagg
     4501 cgatgagaaa gaggatttgg caaacagcag gctgctgaat accgtcaaga aagtcgttcc
     4561 ggtcggcacg gaatttcacg gcgagaaatt ttttaccgtc gaaaacggca aaaaaatcgc
     4621 cacgccgctg tttttggtgc tggtcatgat tgaattgagc gatgtcgtgt ttgccgtgga
     4681 cagcatcccc gccgtctttg ccgttaccac cgatccgttt atcgtgctga cttccaatat
     4741 tttcgcaatt ctgggcttgc gggcgatgta tttcctgctg gcggatgtgg cggaacgctt
     4801 tatcttcctg aaatatggat tggcattcgt gttgggtttt atcggtgtga aaatgctggt
     4861 aatgcattgg gtgcatatcc cgatttccgt ttcgctgtcg gtcgtgttcg gcgcattggg
     4921 tgcatcggtg ttgacttctt tggtttatac gaaaaaaaca ggccgataag cggatataac
     4981 gcccgtccgc ccgcaagggg cttgacaggg tttcggccgg tctgtaaaat tccaaactta
     5041 tcgggtcgtt agctcagccg gtagagcagc ggacttttaa tccgttggtc gagcgttcga
     5101 atcgctcacg acccaccaga taaagcaaaa aagctgagtt ttcaaaactc ggcttttttg
     5161 tcttccgaca atccttgttc ggaaagcgcg gcatttcgtc aaattctttg gtgcggcttc
     5221 cgtatttttc ccgaaaatcc cttataattc cgcctctgca accttcatcg ggtgatgctt
     5281 tatgcgttgt cccgagaatt gaaatattat agtggattaa caaaaaccag tacggtattg
     5341 cctcgcctta gctcaaagag aacgattctc taaggtgctg aagcaccaag tgaatcggtt
     5401 ctgtactatc tgtactgtct gcggcttcgc cgccttgtcc cgatttttgt taattcacta
     5461 tacaagtacc caccaggaaa gaacaaacgt ggacaaactg aaaatctccg caaacggccc
     5521 gctcaacggg gaaataacgg tctcgggcgc gaaaaacgcg gcattgccgc tgatgtgcgc
     5581 gggtttgctg acatcgggta cgttgcgcct gaaaaacgtc cctatgctgg cagatgtgaa
     5641 aaccacgcaa aagctgcttc aggggatggg cgcgcgcgtc ctgaccgaca atatcagcga
     5701 atttgaaatc aacggcggta cggtaaacaa tacctgcgcc ccttacgagt tggtccgaac
     5761 gatgcgcgct tcgattttgg tgctgggccc gacgctggcg cgtttcggcg aggcgcaagt
     5821 cagcctgccg ggcggctgcg ccatcggttc gcgccccgtc gatcagcatt tgaaaggctt
     5881 ggaagcgatg ggtgctgaga ttgttatcga acacggttac gtcaaagcca aaggcaaact
     5941 caaaggtacg cgcgtggcga tggatgtcgt taccgtcggc ggcacggaaa acctgctgat
     6001 ggcggcgacg ctggcggaag gtacgacggt tttggaaaac tgcgccattg agcctgaagt
     6061 ggtcgatttg gcggaatgcc tggtcaaaat gggcgcgaaa atcagcggca tcggtacgtc
     6121 cacaatgatt gtggaagggg tggacgagct gcaaggctgc gaacacagcg tcgtccccga
     6181 ccggatcgag gcggggacgt tcctgtgcgc ggtggcgata accggtggca gggtggtttt
     6241 gcggaatgcc gcgccgaaaa cgatggaagt ggtgttggac aaactggttg aggcaggtgc
     6301 ggtgattgag gcgggcgacg attggatcgc catcgatatg cggcagcgtc cgaaggcggt
     6361 ggacatccgc acggtcgtcc accccggctt ccccacggat atgcaggcgc agtttatggc
     6421 attgaatgcc gtggcggagg gaagctgccg cgtggtggaa acgatttttg aaaaccgctt
     6481 tatgcacgtc cccgagttga accggatggg ggcgaacatc acaaccgagg gcaatacggc
     6541 atttgtgcag ggtgtggaac agctttccgg cgcagtcgtc aaggcgacgg atttgcgtgc
     6601 gtccgccagc ctcgttatcg ccggtttggc ggcgcgaggc gaaaccgtgg tcgaacagat
     6661 ttaccacttg gatcgcggtt atgaaaatat tgaaaaaaaa ctcggcagcg tcggcgcgaa
     6721 aatcgagcgc gtatccggct gatcgggttg cgatgccgtc tgaagccttc agacggcatc
     6781 aattgtttcc gtatcgcaaa cactgattga aaaggactga ttatggcatt tttaaaactg
     6841 accgaacaaa acgtgcaggg caaaaccgtc ctcatccgcg ccgatatgaa cgtaccgttc
     6901 aaagacggca aaatcagcga cgacacccgt atccgcgcct cgctcgcgtc cattaaatac
     6961 tgcgtggaca acggtgcgtc cgttatcgtg atgacccatc tcggccgccc gaccgaaggc
     7021 gagttccacc ccgaagacga tgtcgccccc gttgccgcgc acttgggcag cctgttgggc
     7081 aaagacgtga aagtattgaa cgactggcgt gaaaacaaac ccgccctgaa cgcgggcgat
     7141 gtcgtcatgc tgcaaaacgt gcgcatcaac aaaggcgaga agaaaaacga tttggaactg
     7201 ggtaaagcct acgcttcttt gtgcgacgtg ttcgtcaatg acgcgttcgg aaccgcccac
     7261 cgcgcccaag cctcgaccga agccgtcgcc caagccgcgc ccgttgcctg cgccggcgta
     7321 ttgatggcgg gcgaactcga cgctttgggc aaagccttga aacagcccgc gcgcccgatg
     7381 gttgccatcg ttgccggcag caaagtgtcc accaagctga ccatcctcga atcgctggcg
     7441 gacaaagtcg accaactcat cgtcggcggc ggtatcgcaa acaccttcct gttggcggaa
     7501 ggcaaagcca tcggcaaatc tttggcggaa cacgatttgg tggaagaatc caaaaaaatt
     7561 atggcgaaaa tggcggcaaa aggcggttct gtgccgctgc cgaccgatgt cgtcgttgcc
     7621 aaagcctttg ccgccgatgc ggaagctgtg gtgaaagaca ttgccgccga tgcggaagct
     7681 gtggtgaaag acattgccga cgttgccgaa gacgagatga ttttggacat cggtccgaaa
     7741 tccgctgccg cgcttgccga tttgcttaaa gccgccggta cggtcgtttg gaacgggccg
     7801 gtcggcgtgt tcgagtttga ccaattcgca ggcggtacga aagcccttgc cgaagccatt
     7861 gcccaaagca aagcgttctc gattgcgggc ggcggcgaca cgctggcggc gattgccaaa
     7921 ttcggcgtta ccgaacaaat cggctacatc tccaccggcg gcggcgcgtt ccttgagttc
     7981 ttggaaggca aagagctgcc tgccgtagcc gctttggaaa aacgcggcgc gtaagcggtt
     8041 tgatataaaa aaaatgccgt ctgaaacgaa atggtttcag acggcatttt tgtgtggcga
     8101 ttattgtgct ttggctgccc attccgccgg agtggcggca accgaaatgg aaagtgcgtg
     8161 cagttcgtta ctttccagtt gggctttgag tccgtcttta atcaggcggt ggcgcgcgag
     8221 gcgtgccttg ccttcaaatt ctgatgaaac gatgacggcg aaaaaatggt gtccgtcgcc
     8281 ttctacttcg atatgttcgc attttgccac gccttcaatc atggctttta cttgttccga
     8341 ggtcagcatg gtaggttcct tgttggaaaa atgcgccatt atactggcaa attgaaatgc
     8401 cgtctgaaaa cgggttcgga cggcattttt gacggtgttt tattttttgg ctttgaagcc
     8461 gagcaggtgc tgggtggtgg caagccacgc gccgaatacg cccaacgcga tgacgaagcc
     8521 gaacaccagc ccgagttcgc cagcgtagaa gaaccgccag ccgatattaa gtccgtaggg
     8581 tttgaaaatg gcatcgacca atgggcgcac ggcagagagc agccaaccgc aaagccccaa
     8641 gctgacggcg gcggaaagga tgctctgcca catggcttga taaaggaatg ggcggcggat
     8701 aaacgacgcg ggcgcgccca agagtttggt gatttcgatt tcttctttgc ggctgaggat
     8761 ttgcaggcgg atggtgttgt gtgcgacaag gacgaacgcc atccccagcg tcagggaaag
     8821 aaaccacaaa attttgcgga tgaactcgtt gatttggtac agcgtttgca cccattcggt
     8881 atccatagac gcggattcga ccataggcag tttggtaatg tctcggtaga ttgcctgcat
     8941 ttgggcgggc gtggttgccg ggtcgggggt aacgataaag acatccggca gggggttgcc
     9001 gtcaagcatg gaaatcagat tttggtcgag attggactgt aattcttcca gaccgtcttc
     9061 tttgccgatg aagcggatgt tgtcgagccg tttgtcgcgc gccagcaggc tgcggacggt
     9121 atcgctgtcg ctttgtgcgg cggaggtttc catatagatt gtgatttgcg gcgactcgtt
     9181 gagtttgccc aacacgcttt gcccgctttg gatgcccaga tgcataaaca gcggcagggt
     9241 catcgcgacg gcgagcatca tgagggtaag cagtgtgccg aagggttggc gcaggagctg
     9301 cttgagcgcg gtgcgcgcgg attcgacgtg cagcgagagg tagtggatga tgctcatgcg
     9361 agtcgtcctt tcgagaggcg caggatgcgg tgtccgtagt ccgccatcag ggtttcgtca
     9421 tgtgcggcaa cgatgacggt agttcccgct tcgtggaagg ttttgaacaa ttccataata
     9481 tcgagcgcgt aggcgcggtc gaggttggcg gagggttcgt cggcaatcag caggccgggc
     9541 tggtgaacga cggcgcgggc gatgcacagg cgttgttgtt caccgccgga gagggttacg
     9601 ggatcgtcca attctcgtcc tttcaggccg actttttcga tggcgatgcg ggcacgctct
     9661 tcggctttgc gcggcggata gccgataatc cgaagcggca ggatgacgtt ttgcaggacg
     9721 ttgcggtcgt agaggatttt gtggtcttgg aacacgatgc cgatgtgttg gcgcataaag
     9781 ccgatttggt tgtcggacaa tgtgccgagg tcctgcccgt taaacaggat tttgcccctg
     9841 ctcggcttgg taatgcccga aatcagtttg aggatggtgg atttgcccga accggagtgt
     9901 cccgcgataa atatcatttc gcctttgttg atttggaagc tgacgttttt cagggcttca
     9961 aaaccgccgg gataggtttt ggaaacttgt tcgaaacgga tcataggagg tcctgtaagg
    10021 aagggcggca tatcgtttcg ggcggttgcg tccgcaggga tatgccgtct gaacaatagg
    10081 taagacagtg ccgattataa agattccgcc tgtttttaga tagtggataa gtttgcaccg
    10141 gcaggcgggt ggaaaagaaa aatgccatct gaagcggctt cagacggcat ccggcgttta
    10201 acggcatttt gaatgggcga gtttgacggc gtcggtcagg cttttttcgt ttacctcccc
    10261 ggtaatggtc tgcctgtatc cgcatttcgg tgcttcgacg acggtaaagg gcagtacgcc
    10321 gacagtgttt ccgtaggttt tcataaagtt tcggctgttc gccccggtgt aacgccaaat
    10381 cgggtaggaa acaggagttt gtttgaggaa gttgccgata ttgtcggatg tgtcgagcgc
    10441 gatgccgacc atatcgacgc tgcctttttt ctgcgctttg taccatttgg acatggcagg
    10501 catctctttt cggcacgggc cgcaccaagt cgcccaaagg ttgacgatgc gtacgggggc
    10561 tttgagcgat tgcaggcttt gcggggtgtt gtctttccat ccggccagtt cgtctgccga
    10621 ggcggtatgt gcggcaaatg tggcggcaag ggtgattgcg gcaagattca agtatttcat
    10681 cgttttgtcc tttgtatgtc gattcgggta ttgtaaaccg tttggtacgg ttgtaggcgg
    10741 gatattgagt aaaaccctgt gtaagccgat aataagggga ttgtcgagaa gcaaggaaat
    10801 gtcatgcctg aaatcaaaat tttccacaat ccgcgttgca gcaaatcgcg tgccgccttg
    10861 tccctattgg aagaacgcgg cattgctgcc gaagtggtca aatatttgga tacgccgccc
    10921 gacttgtccg aattgaagga tattttcaac aaattgggct tggcatcggc gcgcgggatg
    10981 atgcgcgtga aagatgattt gtataaggaa ttgggtttgg acaaccccaa tttggacaat
    11041 gacgcgctgc tgcgtgccat cgccgatcat cccgccctgt tggagcgtcc gattgttttg
    11101 gcaaacggta aggcggctgt cggcagaccg ctggaaaata tcgaagcggt attgtgaaca
    11161 aacggctgtt ttgcagccgc aacggcttgc gctattacct gttgggcggt ttctgtttga
    11221 gtgtttttcc gcttttgctg gtgtttgcct catccgtttg ggcggtttat cggacgggtg
    11281 ggcaggtttt gccgccgtat gtccgggcgg atgccgcgct ggtgttgggc gccgccgcgt
    11341 gggacaaacg cccttctcct gttttccgcg aacgcatcaa ccatgccatc gcactttatc
    11401 agagccgcag ggtaggcaaa atcgtgttta cgggaggccg gaccaaaaaa ggctatatga
    11461 ccgaagccga ggtcgggcgg cggtacgcac tcaaacaggg ggttccggcg cgcaacatcc
    11521 tgtttgaaaa cacttcgcgc aacacttatg aaaacctgaa caacatccgt ccggttttgc
    11581 gtgccaacgg tattgccagc gtggtgattg tcagcgatcc ctaccatttg gcgcgggcgg
    11641 cggaaatggc ggaggatttg ggtgtccgcg tttatatgtc tgccacgccg accacgcggt
    11701 ttgatgcggg aaataagaaa aaaatcttta tgctgcaaga gggatatgcg ttatccctct
    11761 accgtttgga aaaatggggg cgggattttt tcaattagct gtttcattga actttttttt
    11821 ggacgcaatc ccgaaggaat tcggtcggcg gcgcgtgata cactgttccg cgtgccgaag
    11881 cattgaaaac aaggaaaatg ccgtctgaaa ctttatccgc aggcagggtt cagacggcat
    11941 ttttgatgcc caagcacggt aaaaccagta aaataaacgc tttcaaagat ttcggagcca
    12001 tcatgaccgt caaaacccgt ttcgccccca gccccaccgg ctacctgcac atcggcggcg
    12061 tgcgcaccgc cttgttttca tgggcgtttg cccgccatca taaaggcgag ttcctattac
    12121 gtattgaaga caccgacttg gcgcgttcta ccgccgaatc cgtcaacatc atcctcgacg
    12181 gcatgaaatg ggtcggtctc aattacgaca acgccgacaa cgtcgtttac caaactcgcc
    12241 gtttcgaccg ctacaaagag gtcattgccg aacttttaga aaaaggccat gcctactact
    12301 gctattgcag caaagaagag ctggaagcca tgcgtgagaa agccgaaaaa gaaggctcgg
    12361 cgacttacga ccgccgctgg cgtccggaag taggtaaaac cctgcccgaa atcccgtccg
    12421 acgtacaacc cgttgtccgt ttcaaaacgc ctttggacgg tgtgaccaaa tggacagact
    12481 tggtcaaagg cgaaatctcc atccccaacg aagcactcga tgacctgatt atcgcccgcg
    12541 ccgacggtac gccgacctac aacttctgcg ttgtcgtaga cgactacgac atgggcgtta
    12601 cccacgttat ccgcggcgac gaccatgtga acaacacgcc gaaacaaatc aacatcttaa
    12661 aagccatcga cgcgaacctg cccgaatacg gccacctgcc gatgattctt aacgaacaag
    12721 gcaaaaaaat ctccaaacgc agcggcgata ccgttgccat taccgatttc ggcgcaatgg
    12781 gcatcctgcc cgaagcgatg ctcaactatc tggcacgctt gggctgggcg cacggcgacg
    12841 atgaattctt cacaatggaa caattcatcg aatggtttga tttgaaagac gtttccccgt
    12901 ctccaagccg tatggacttg aaaaaactct actggatcaa cggcgaacac atcaaaatca
    12961 cacccaacgg caaactcgcc gaactcgtca aaccccgcct tgcgttgcgc gatattcatg
    13021 aaaccgaaaa acctgctttg gaagatgtgt tggaactggt caaagaccgc ccccaagact
    13081 tgaacacgct tgccgacgag tgcttctact tctacgtcaa acaaacgcct gccgaagcag
    13141 acgtgcagaa acactgggac gatgaagccg ccgcccgtat gctgcgcttt gccgaacgcc
    13201 tcgaagggct ggaagactgg aatgccgaag ccatccacga cctcttcaaa cccttctgcg
    13261 acgaagaagg cattaaaatg ggcaaactcg gcatgcccct gcgccttgcc gtctgcggta
    13321 cggcgaaaac ccccagcgtc gatgctgtgt tggcattaat cggcaaagaa gaagtgttga
    13381 aacgcatccg cgcttaagtt atccacaggc gtgggcgatg ccgtctgatg tcggaatacg
    13441 gcttcagaca gcatttaaat ggaagttaaa aatgaaaaaa atattttatt ttctgatggt
    13501 tgttttttct acaagcgtat gggcagggga tgctgaagac aatctgctca gcatccaatc
    13561 cggttaccgc gccttattgc aaaagcaaaa caatctggac ggaaaaatca tcgggatgca
    13621 gtcggatttg gaagatgcgc gccggcgttt gcagacggct caggcagaca tcgcccgttt
    13681 ggaagcggaa attcctgcag caatggcgca aaaagcccgg caggctgaag atttgaggca
    13741 aatcggagtg cgtttggacc atgcttggaa tgcggtttac ggcgcagggg gaacgaaggc
    13801 gtcggggaat tgaccgatga attccctctt tgtggacaat actgttttca ttacacggct
    13861 gaaagccggg catatcggca ggttggttca ggcgttgttt gaggagtggc acggatttga
    13921 accgtggtct tctgtggata agattcatgc ctattacggc aggtgtttga aggatgacga
    13981 actgccgctg gcatttgcgg ctgtggatga ttccggaatc ctgttgggtt cggctgcggt
    14041 caagcggcat gatatggaaa gttttccacg gtatgaatat tggttggggg atgtctttgt
    14101 tttacctgaa tatcgcggaa aaggcattgg caggaggctg gtcgcccact gcataggcgc
    14161 agcgcgttcg ctggggataa agttcttgta tctttatacg cctgatgtgc aaatatttta
    14221 tgaatcattc ggctgggtgg ttgtcgggcg acatttccat aacggtgaat gggttacggt
    14281 tatgcgtttg gatgtggata aggtttaagc cgaatggacg gatgccgtct gagctgttcg
    14341 gacggcattt tttatgtttg gaaatttcct gtcagataat gtttatccac agatttttcc
    14401 atatggtttg atgctgttcc gacaacggaa attaagatac ggatttgcac aaatctttca
    14461 gacggtacag ttctgacaat gcttcgcgcg gggtcaggct gtcgggatcg agtttttcca
    14521 aggctgctgc caatatacct tcaaaatgtt tttcctctgc tttatccaca aagttgccca
    14581 cattcggttc atctcctttt tcagacggca tggtactgaa aatatccagt tggggacggt
    14641 tcgcggcggc ttggttttcc agtccgttca aatgcttttg ggcggatttc aatgcgcgta
    14701 caggcaggcc ggcgagtttg gcgacggcaa tgccgtagct tttaccggcg ggacccggtt
    14761 ggatttggtg caggaaaacg atgtcctgtc cctgttcgag cgcggaaagg tgcatattga
    14821 cggcggcggt gtgggcttcg ggcaggtagg tcagctcgaa atagtgggta gcaaacaggc
    14881 tgaaggattt gtttttttgc agcaggtgtt cggcaacggc gtgcgcgagg gcgaggccgt
    14941 cgaaagtgga agtaccacgt ccgacttcgt ccattaaaac aaggctttgt tcggtggcgt
    15001 gatgcaggat gtaggcggtt tcgctcattt cgaccatgaa agtggagcgg ttggaggcga
    15061 ggtcgtccga tgcgccgatg cgggtgaaga tttgatcgat gggcccgatt gtggcggcat
    15121 cggcaggcac aaaacagccg gtgtgtgcca ataaaacaat cagcgcgact tggcgcatgt
    15181 aggtggattt gccgcccata ttggggccgg tgagcagcat gaggcggtgt ttgtggtcaa
    15241 ggtcggtgtg gttggcggtg aagtggcgta cctgctgttc gacaacggga tggcggccgt
    15301 tttcgatgtg gataaccgga tagtcggcaa actcggggcg gacgaagttc cgctcttttg
    15361 ccaaggctga aaatgtggac aacacgtcca gcgcggcggc ggctttggcg gctttttgaa
    15421 gctgcggcaa tgccgtctga aggtttttca atacgccgtc aaagagttgt ttttctaagg
    15481 cgagggcttg ctcttgagca gtcagcactt tgtcttcaaa ggctttcagt tccggcgtga
    15541 tgaagcgttc ggcgttttta agggtttgcc ggcgttggta gtcggcaggt gcttgttcgg
    15601 cttgggtttt ggacaattca atgtaaaagc cgtgaacgcg gttgaactcg actttaagtg
    15661 tggacaaacc ggtacgttcg cgttccttgg cttccaaatc cagcaaaaat tcgtcgccat
    15721 ggttttgaat gcggcgcaat tcgtccagtt cgggatgaaa accgtggttg atgacattgc
    15781 cgtctttcag ccagacggaa ggttcgggca aaatggcttg gcggagctgt tcggctgtgg
    15841 ataggttttc cgggaaaacg gctttgaggg ttcctaagag actgctgcac tcggcggaca
    15901 attcgatttc ggacagggca aacaggctgt cgcgcagggc ggcgaggtcg cgcgggcggg
    15961 cgttacccac ggcaatacgg gcggcgatgc gttcgatgtc ggcaatgctt ttcagacggc
    16021 actggagggg tttgtattgg ctttccagcg cggcaacggc ttcttggcgc gctcggatgt
    16081 gggcgcggtt gcgtaaaggg tggtgcagcc agagagccaa gaggcggctg cccatatggg
    16141 tagcgcaaag gtcgagcgtg gacatcaggg tcggcgattt tttgccggag agggtttgcg
    16201 tgatttcgag attgcggcgc gtggcggcat ccataccgat atattggctg tcggtttcga
    16261 gcgacaggcc gtccaaatgt tgcggcatca ggttttgcgt cagacggata tagttcaaca
    16321 gtgcacctgc cgcgccaatc gcaacggcgt gttctttgcc gtccaaaccg aagccgcgca
    16381 aatcctggca gccgaaatat tccgtcagca gtttttcccc cgcgtcggcg gcaaactgcc
    16441 acgcgttcag gcgcgtaaca cccgatgccg tctgaagttg cggtgcgttt ttactgtcag
    16501 gcaacagaat ttccgccgcc tgcaggcgcg ccagttcgtc gtccaattta tccacagttg
    16561 tcagcttggt tttgaattcg ccgctttgca gcgatgccca cgccaaaccg atgtattttt
    16621 tgtcggggga cacggcaacg atgcggttgg tttccttgtc ttccagcaat gcggaatcgg
    16681 tcagcgtgcc gggcgttacg atgcgcacga ctttgcgctc cacaggccct ttgcccgcgc
    16741 cgacttcgcc gacctgttcg caaatcgcca cgcttttgcc caacttgacc aggcgcgcca
    16801 gatattgttc ggcggcgtga aacggcacgc ctgccatttt gaccggctcg ccatccacct
    16861 gtccgcgcgt ggtcagggta atatccaaaa gttttgccgc ttctaccgca tcgtcgaaaa
    16921 acatctcgta aaaatcgccc atacggtaaa acaccagttt gtcggtatgt tgcgctttga
    16981 tgccgaggta ttgctgcatc attggggaaa cggcggattt ggacatgggg ctgccttatc
    17041 attaggaaaa cggtattgta gcaaaaggtt ttgtcttcga cggaatgaga ctgaccgtaa
    17101 gcctgtcaat aaggttgtgg agacaactag gaaaacagca aagttatcca cagaaatatt
    17161 attaagtcaa aaaacatcca cgcttatctg tcttatacag caagtcttaa actcaaactt
    17221 atttgacaaa aaggtaatga aaatattgga tatttgctac ttacgcacaa gcaggttggc
    17281 aatcatcaaa atcatcatta aaattttaaa ttcaaatatt gatggtaaaa gttttggaaa
    17341 tctgtgcaaa cagtaaaatt catctatatc aaataagcgg caagtagtta gatgtaatag
    17401 tggaatgtaa ctttatttca gaatacaata caggtattga aatttcgttt cacagcttgg
    17461 agtatataca tgagcattaa agtagcgatt aacggtttcg gccgcatcgg ccgcctcgca
    17521 ttgcgtcaaa ttgaaaaagc ccacgacatc gaagtcgtcg ccgtcaacga cctgacccct
    17581 gccgaaatgc tgctgcacct tttcaaatac gacagcacac aaggccgttt ccaaggtact
    17641 gccgaattaa aagacgatgc catcgtggta aacggcaaag agatcaaagt ttttgccaat
    17701 ccgaatcccg aagaattgcc ttggggcgag ttgggtgtgg atgtcgtcct cgaatgtacc
    17761 ggcttcttta ccaataaaac caaagccgaa gcacacatca gggccggcgc gcgcaaagtc
    17821 gtgatttccg cacccggcgg caatgatgtg aaaaccgtcg tatatggcgt aaaccaagac
    17881 attttggacg gcagcgaaac cgttatctcc gccgcttcct gcaccaccaa ctgccttgcc
    17941 ccgatggcgg cagtcctgca aaaagagttc ggcgtagtcg aaggtctgat gaccaccatc
    18001 cacgcctaca ccggcgacca aaacaccctt gacgcgccgc accgcaaagg cgatttgcgc
    18061 cgcgcccgag ccgccgcgct caacatcgtg cccaacagca ccggtgccgc caaggccatc
    18121 ggtctggtta tccccgaatt gaacggcaaa ctcgacggct ccgcccaacg cgtccctgtt
    18181 gccagcggct cgctgaccga attggtttcc atcctcgaac gccctgtaac caaagaagaa
    18241 atcaacgccg cgatgaaagc cgccgccagc gaatcttacg gctacaacga agatcaaatc
    18301 gtttcttccg acgttgtcgg catcgaatac ggctcgctct tcgatgcgac ccaaacccgc
    18361 gtgatgaccg tgggcggcaa acaattggtg aaaaccgttg cctggtacga caacgaaatg
    18421 tcctacacct gccaactcgt ccgcactttg gaatacttcg caggcaaaat ctaaggcttg
    18481 aacaaacctg tggataagta aatgccgtct gaacacattg cgttcagacg gcattgttta
    18541 tctgtatagt ggattaaatt taaaccagta cggcgttatc tcgccttgcc gtactatttg
    18601 tactgtctgc ggcttcgttg ccttgtcctg atttaaattt aatccgctat aacttgatat
    18661 tgcggcttat ggcgaacaaa caacagccgt ttgttcaact gctatgcggc aaaaccggat
    18721 ttttgcccga tttaaagcgg ggttggggaa agatcaataa cggttgcacg gttttcctcg
    18781 atttgaaatc tgacttcgta atctgcaata ttcatcacat aaatccgttc gggaatattc
    18841 tgataggcgg ggcgcggatc ttgggcaatg ctttggctga taaggttttt ggtgtttgca
    18901 gataaatttt ccgcgccgat gttttcctgc caaacgactt ccaactctac gggtttgccg
    18961 ctgacgaaac cggatgcggc atcgggtttg gattcgacaa aggggatata aggtttgatg
    19021 tccacaatcg gtgtgccgtc cagcaggtct gcgccgctgc aatagaggcg gacgggtttg
    19081 ccggtttcga tgcgttcgag tttcaggagc gagagtccga gatggttggg gcggtggggg
    19141 ctgcgcgtgg cgaacacgcc cattttttgt ttgccgccga gccgtggcgg gcgcaccatt
    19201 tgcgcccagc cttcatccaa tacgccgtga aaaataaaac ttatccacac ataatcgaaa
    19261 tcttccagcc cgcgcacgct gtctgcggtg aatttgggat tcagctcgat gcaggctttt
    19321 gcggcggaga ccaaaccggg ctggcgggcg atgccgaatt tctgtttgta gggcgagcgg
    19381 gcggtgccga tgggggtaat ggtataagtc ataaggttgt ggataagttt tttggaaaaa
    19441 ccttatctta tcacaatcgg ttttaccgct tttgggcagt aatttgttat aatacagggc
    19501 attttaatcg cgttgaaata atgagatgat tgtttccatt gatgttgatg cacaaaaaac
    19561 gtttacgcca ctgtgtcctg acgaactgcc cgtgaacgag gggcatttga ttgtcgagga
    19621 gttgaatgcg caagccgctt tggcggattt gcgcgtgatg acgaaagatg cgcatcatat
    19681 ggtagcgaaa tggcttgtgg ataaccctgt tgatatgttg aagccgacag gtttgcctga
    19741 tgcggatttg acttgggtgg ctcatgcgat ggtcggtacg cgcggctatg aattattgga
    19801 cgggctgcct tctgctaaag aatacgatta ttgcgtttgg aaaggtgttg atcctgaatt
    19861 gcatccttac ggcgcgtgtt ttcacgatat tgaggaaaaa ctaagcacag ggctgattga
    19921 atggctgcgt tgtcaaaata cggatacggt tattatcggt gggttagcta cggattattg
    19981 tgttaaaaca acggttttgc agttactcaa aggcggtcgt tggcaggttg tcgtcaatga
    20041 agcggcttgt cggggtattg cgccggaaac catcgaaaca gcatggcagg aaatgcattc
    20101 tgctggggca atcatcttaa aaaacgctga tgaaataaat aaatatatta ataatcaata
    20161 agttaaaatt gtttcacgtg aaaccccctt cctaaatatg aaaattttgc ttgtccgctt
    20221 gtctagtatg ggcgatttaa ttcacacttt gcccgcaatc gaagatttgg cgcgacaatg
    20281 tcctgatgta gaactgcatt ggctatgtga ggctggattt gcagatattg cgcgcctgca
    20341 tccgtttgta aaaaaaatcc atgtgatgaa atggcggcaa tggcgcaaac atctctttcg
    20401 ggctgaaact tggcgggaaa tgggtcatct gaaacagact ttgcggcagg aagtatttga
    20461 tttcgtattg gacagtcaag gtctgattaa aagcgcgtgt ttcgctaaaa tggcaaaatc
    20521 cccgatttgt ggtttggata aaaacagtgc gcgcgaggga tgggctgctt tggcgtatgt
    20581 aaaaacatac gctgtaccga agggaaaaaa tgccgtttgg cgcaaccgtg aactgtttgc
    20641 ccaagtattt gggtatgtaa tgccggaaac gcaggtattt ggcttgactg ttcctgaagc
    20701 aggccgtctg aaaaatttag cgcagccgta ttatgcggct ttgcatgcga ccagtcggga
    20761 cagtaagtta tggcctgtgg aaaactggcg gtcgttgctg caaaagttga atgaagaaca
    20821 gcaatgcaat atttacctgc cttggggaaa tgaagatgaa aaaacgcgtg ccaaacaaat
    20881 tgcagatggg ctgccgtttg ctattgtgtg cgacaaaata aatttattgc aggcagcgta
    20941 tctgctgaaa tacgcggtcg gaattgtcgg cgtggatacc ggtttgctgc atttggcaaa
    21001 tgccttggaa aaacctgtgg tcggcattta taccgatacc gatccgatta aaacaggcgt
    21061 tcaggtttcg ccaattgcaa aaaatttggg caatatcggg cagattccga ccgcagattt
    21121 ggtttatcaa gcgctgatgg attgtgttgc agcagataaa ggctaaaggg tcgtctaaaa
    21181 ctgatatggc ggcatgatat tttccaattt attagataca ggaatgtata tccgctagtc
    21241 tgagtaaatg ctttaaaaat ggtataatcg gcaagcatta tccgaaagat ttttgaatga
    21301 attcgttttt aaggaaaacg gatttggttt tatagggaag aaggtttggt ttcgctggat
    21361 ggtgaaagca gcgttctttt taggagggtt caatgaaagc actggtcgca gtaaagcgcg
    21421 tagtggacta caacgtcaaa gttcgtgtaa aagccgatgg ttcggatgtg gatatcggca
    21481 atgtcaaaat gtcgatgaat ccgtttgacg aaatcgctgt ggaagaagct gtccgtttga
    21541 aagaagccgg aaaagtaagc gaaatcgtag cggtttcttt gggtgagaaa aaatgcgaag
    21601 aaaccttgcg tacagctttg gcgatgggtg ccgaccgtgc cattcatgtt gaaaccgata
    21661 ctaaactgga gtctctggcc gttgctaagt tgctgaaagc cgttgcggac aaagaaaatc
    21721 cgcaaatttt ctttttgggc aaacaagcga ttgatgatga tgccaatcaa gtggcgcaaa
    21781 tgctggcagc tttgctgaat gcggcgcaag gtacgttcgc gtccaaagta caaattgaag
    21841 gcgacgaagt gcagattgtg cgggaaatcg atggcggcga agaaaccatc gcattgaaac
    21901 tgcctgctgt tatcagcgcg gatttgcgtt tgaacgagcc gcgctttgtc aaactcccca
    21961 atattatggc ggcaaagaaa aaacctttgg aaaaactgac tcctgacgat ttggttgccg
    22021 acatttcacc tcgtttgaaa acggtgaaat ttgccgaacc taaggcgcgt caggcaggcg
    22081 taaaagtagc aagcgttgcc gaattggttg aaaaattgaa aaacgaagcc aaagtgattt
    22141 gaggaggctg aaatgagtgt attgattatt gtcgaacacg acaacaaaca gttgaatcct
    22201 accactttgc atgctgttac cgctgccgcc aaactgggca aagtcgattt attggttgcc
    22261 ggaaacggcg catctgctgt agtggaattc gcgaagcaag tagcgggggt gaaaaaagtt
    22321 ttggttgcag atgctgctca ttatgccgaa ggtttggctg aagagttggc tccgctggtt
    22381 gttaaattgg cggccgatta ccgctatgtt gcagcaacgg caaccacatt cggtaaaaac
    22441 cttttgcccc gcgtagcagc cttattagac gtaccgcaaa tttctgattt gaccgaaatt
    22501 gtggataaca cgacttttgt gcgccccatt tatgcgggta atgcatttga aaccgtgcaa
    22561 gctgattcag aaaaattggt gctgaccttc cgtgcgacgg tttttgacgc agtggcggcg
    22621 caaggtggta atgctgaggt aattaatgtt gaagcaaccc ctgcccaaaa cctgagtcgt
    22681 tttgttaacc gtcagctttc ccattccgat cgtcctgaac tgactcaggc aaaagtgatt
    22741 gtttccggtg gtcgtgcgtt gggtagtgcg gaaaaattca atgaagtgct gacaccgttg
    22801 gcggatgttt taggtgcggc aatcggtgca tcccgtgcag cagttgatgc cgaatatgcg
    22861 ccaaacgatg ctcaagtcgg acaaaccggt aaagtggttg cgccgcaact ctattttgca
    22921 atcggtattt caggtgcaat tcaacacgtt gccggtatgc aagacagtaa ggtgattgtt
    22981 gcaatcaata aagatgctga tgcgccgatt ttcaatgtag ccgattacgg attggttggc
    23041 gatttatttg aaatcgttcc acaattaaca gaagtattga aaaattgatg tttcacgtga
    23101 aactttcaga cgaccttgtt attaatgagg tcgtctgaaa acatttcagc atgattacga
    23161 cttataaaac cattacttcc tcgacgcagg ctgagtttaa agataaaggc agccgtttta
    23221 ttgcatttgc ctatccgatt cggacattgg ctgatgtgaa aaaatacctc gatccgttaa
    23281 aggaagagca tcataaagca cgacactggt gctatgccta tcgtttgggt gtggatggtg
    23341 tgcaatttcg tgccaacgat gatggagagc catcaggaag tgctggacga ccaattttgg
    23401 gacagattga ttcggtgggt attaccgacg ttttggttgt ggtcgtccgc tatttcggtg
    23461 gtactttgtt aggtgttcca ggtctgatac atgcgtacaa agaggcgacg gctcaagcgt
    23521 tggcagttgc agaagtagtt gaaaagaata ttgaaaaaac tgtttggctg aaatgtgaat
    23581 atccgttttt gaatgaagcg atacgcattg ccaaacaata tcaggcggat atattggagc
    23641 gggatttgca gttggattgc cgattgacgg tcagtttgtc gttggtaaat tatgaggcct
    23701 gtgttgcggc ttggaagaat actcggcaga ttgaagtaaa tacggaaaag ccttttgaat
    23761 gaaaggtcgt ctgaaatgct ttgtgatggt atttcagacg acctcttcgc tgactaatct
    23821 tcttgtgtat atttgtctaa ggcatcgacg ctggagcgca aatataacca agccaattga
    23881 tggaattcgc ccagcgcgtt ccatagcgca tcttcactca taatgctgta ttcgccttcc
    23941 gtactcaaat ccagttctac cccgtcttca atagcctgat gacacgcctg atactcgtgg
    24001 gtatagaaat tgtccatgtc gcgaattaaa gtgacgacat gtttccaacg gcggatgtct
    24061 tcttccagtt gcggtagtaa atgacaccat tcgcggtatt tgctttggat ttcatcaata
    24121 cgtttttgca tggttgcttg tcctttttta atttatggat aagttgaata ggttgcagag
    24181 tggaatgaag cggtcttttg tttgattcta ctaaaatttc ttcagatggg ataaaagtcc
    24241 ctttgtatcg agttttcgtt aaaagtacat atttgtcaag gagatggtat gaaactgctg
    24301 gttatcggta atggcggtcg cgaacacgcg ctggcttgga aattggcgca gtcgcctaaa
    24361 gtggaaacag tatttgttgc gcctggtaat gccggtacag cgattgaacc caagttgcaa
    24421 aacatcgact tgactgcgca tcaggatttg attgaattct gtcgtaaaga aaatattgtt
    24481 tttaccgtcg tcggccctga agcgcctttg gcggcaggta ttgtggatga tttccgtgct
    24541 gcaggactaa aaatattcgg tccgacacaa tatgcggcgc agttggaaag ttccaaagat
    24601 ttcgccaaag catttatggc gaaatacaat attccgaccg cgcaatatca aacctttgaa
    24661 aacgccgatg ccgcacatga ttacgtcaat cagaaaggtg cgcctatcgt tatcaaagcc
    24721 gatggtttgg cggcaggtaa aggcgtgatt gtggcgatga ctttggatga agcgcatgct
    24781 gcgattgatg atatgctgct ggacaacaaa atgggcaatg ccggcgcgcg tgttgtaatt
    24841 gaagatttcc tgcaaggcga agaagcgagt tttatcgtca tggttgatgg caataatgta
    24901 ctgcctatgg caaccagcca agaccacaag cgtcttttag acggcgacaa aggtctgaat
    24961 acgggcggta tgggtgcgta cagccctgcg cctgtggtaa cgcctgttgt gtacgaacgc
    25021 gcgatgaatg aaattatttt gccgaccgta gcagggatga aagcggaggg gcatgagttc
    25081 accggtttcc tgtacgcagg tttgatgatt gatcaaagcg gtgcacccta tacgattgag
    25141 tttaactgcc gtttcggcga tcctgaaacc cagccgatta tgagccgact gaacagcgac
    25201 ttatcggatt tggttgaagc ggcaatagac ggcaaactcg atagcgtaac cgcggaatgg
    25261 agcccgcaaa ctgccgtggg cgtggtactg gcggcgcaaa attaccccga aacgcccaaa
    25321 aaaggcgata ttatttccgg tctggatgct gccaaccaaa tcggcaaagt tttccatgca
    25381 ggtacaacgg caaacgagaa aggtgacgta ttgaccaacg gcggacgcgt attgtgtgtt
    25441 gtgggattgg gcgataatgt ggcgcaagcg aaagctaagg catatggcgc gttggaaaaa
    25501 atcagtttcg acgggatgca ataccgtaaa gacattgccg ataaagcgat taaccgttaa
    25561 ttagaaagag gatgatgccg tctgaagcca agtatgacta atgtgcttca gacggtattt
    25621 tatattatgt tatttccgag aattatcgca gcatcagcgc aacgtaagtt aagcgtttat
    25681 ttgaaaaaag gtggtttggt cgcttatccg accgaatctt gttatggctt ggggtgtctt
    25741 ccgacattag ccaaagcact cggtaagttg gcgcatttga aaaaaagacc gcaacacaag
    25801 ggtatgattg tcatcgggaa tcaatttgaa caattgcagc ctttacttca aatgccgtct
    25861 gaaaatcttc aggatatgct tagaaaagaa tggcccgccc ctaaaacgtt cctattatcc
    25921 gccaaatcct gtgtcttacc cgagttacgc gggaaacagc gcagcaagtt ggcggtcagg
    25981 gttcccgccc atgtaggtgc gcgccgcttg tgtcaggctt tgcaaacgcc tttggtctcg
    26041 acttcgtgca accgcgcagg caaacgcgcg tgtcggacgg agagggaagt cagaaggcaa
    26101 ttcggacgcg atgtgtggat tgttggtggg agaatcggca ggcagaaatc gccgagtcag
    26161 attattgacg gggaaacagg taaacgtttg cgttagatat gggtacttga atgaaaactt
    26221 tttttgcata cacaaagaaa aaatttttcg gatagcattt ctctacatga ttgttattgc
    26281 acagagataa aggtagaagg acgcaacgtc tgctttaatt ttgaggacgg ttttgtcgtt
    26341 ccggacaata atccgaacaa tcaagcgggc aagcatctga aaacagattt ttcccgtgtt
    26401 gttttaatcc ataaaaatga aaacgccgaa gatgatta
//
LOCUS       DAWGRZ010000008        25462 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000008 DAWGRZ010000000
VERSION     DAWGRZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25462
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.008"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..347)
                     /locus_tag="WHT44_000242"
     CDS             complement(<1..347)
                     /locus_tag="WHT44_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HEZ2532481.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDG"
     gene            complement(344..1486)
                     /locus_tag="WHT44_000243"
     CDS             complement(344..1486)
                     /locus_tag="WHT44_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ2532482.1"
                     /translation="MGKSLTEIAEELKGNDKKVQLIYAFNGTGKTRLSREFKNLIAPT
                     SSEEPDGEPTRRKFLYYNAFTEDLFFWDNDLLANEAPRLKIQKNSFTDWLLRDNGLDG
                     AVIKNFQYYTDDKLTPDFNDDFSEIAFYFARGNDEQIENIKISKGEESNFIWSIFYVL
                     IRQVIAELNIPEDSEEGRSTDQFDDLEYIFIDDPVSSLDENHLIQQAVDLADLIKLSK
                     PRLKFIITTHNVLFYNVLYNELKKLEKEKKSYLLLKNEDGSFDILEKQGDSNKSFSYH
                     LHLKGVIEKAIENQQVERFHFMLLRNLYEKTANFLGYKQRSDILPEDSRRNYFQRIIN
                     FTSHSTLSNEAFAEPTPQEQETVKLLLQHLLDNYNFFQDDEQRDKP"
     gene            complement(1544..2149)
                     /locus_tag="WHT44_000244"
     CDS             complement(1544..2149)
                     /locus_tag="WHT44_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ2532483.1"
                     /translation="MLTIKSGGIADGYKGRLKDVVWKTLGEVFNIFAGGDVPKDAFSE
                     VETEEFCIPILSNGIGSKALYGWTNVAKINQPSLTISARGTIGWASFQNKPFFPIVRL
                     LVLTPKIELNLKYAYYFMKSIESNYKVPESGIPQLTKPMIKDISIPIPPLPEQEKIVA
                     ILDKFDTLTHSISEGLPYEIALRRKQYEYYRGQLLSFPKAA"
     gene            complement(2217..2705)
                     /locus_tag="WHT44_000245"
     CDS             complement(2217..2705)
                     /locus_tag="WHT44_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ2532484.1"
                     /translation="MDMQNKAKKLIEMIQTAPVEWKPLGEVAKVLRGKRLTKKELIEG
                     GKYPVFHGGLIPLGWFDQFNRRANQTMIINTGSIGEVIWSGVDFWSSDGTFVIQTPNY
                     LDDKFIFYFLKTREGYIKSQKRVGGVPTIDRLVVENISIPIPPLETQQKIVKILDKFT
                     EL"
     gene            complement(2871..3881)
                     /locus_tag="WHT44_000246"
     CDS             complement(2871..3881)
                     /locus_tag="WHT44_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEZ2532485.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(3878..5422)
                     /locus_tag="WHT44_000247"
     CDS             complement(3878..5422)
                     /locus_tag="WHT44_000247"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEZ2532486.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGNFENHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNQF
                     HIELGDTLTNPKLKDSKPFDAIVSNPPYSINWIGSDDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDASGFFKKETNNNVLIEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEAEDTREIIDIKQLNAEIGETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(5496..6500)
                     /gene="rfaD"
                     /locus_tag="WHT44_000248"
     CDS             complement(5496..6500)
                     /gene="rfaD"
                     /locus_tag="WHT44_000248"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ2532487.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMDNNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYKEEFFDVKSGVDRYVKWMLENLA
                     "
     gene            complement(6629..7660)
                     /locus_tag="WHT44_000249"
     CDS             complement(6629..7660)
                     /locus_tag="WHT44_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ2532488.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPFIQKNCFFYKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMSQCPFTLITDEILLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKN"
     gene            complement(7662..8897)
                     /gene="dcm"
                     /locus_tag="WHT44_000250"
     CDS             complement(7662..8897)
                     /gene="dcm"
                     /locus_tag="WHT44_000250"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ2532489.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFSGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGAKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(8934..9905)
                     /gene="hldA"
                     /locus_tag="WHT44_000251"
     CDS             complement(8934..9905)
                     /gene="hldA"
                     /locus_tag="WHT44_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ2532490.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(9960..10700)
                     /gene="pyrF"
                     /locus_tag="WHT44_000252"
     CDS             complement(9960..10700)
                     /gene="pyrF"
                     /locus_tag="WHT44_000252"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ2532491.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(11226..11873)
                     /gene="adk"
                     /locus_tag="WHT44_000253"
     CDS             complement(11226..11873)
                     /gene="adk"
                     /locus_tag="WHT44_000253"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ2532492.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQAVEAVKAEVLGALGK"
     gene            12089..12928
                     /gene="htpX"
                     /locus_tag="WHT44_000254"
     CDS             12089..12928
                     /gene="htpX"
                     /locus_tag="WHT44_000254"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ2532493.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(13229..13789)
                     /locus_tag="WHT44_000255"
     CDS             complement(13229..13789)
                     /locus_tag="WHT44_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532494.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(13903..14295)
                     /locus_tag="WHT44_000256"
     CDS             complement(13903..14295)
                     /locus_tag="WHT44_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ2532495.1"
                     /translation="MGKRMTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQ
                     LLYYDTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALN
                     TPYGSIRTKLDGRSNPDNLVCNRYKVLL"
     gene            complement(14503..14895)
                     /locus_tag="WHT44_000257"
     CDS             complement(14503..14895)
                     /locus_tag="WHT44_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ2532496.1"
                     /translation="MAKSFNQAASELTDIFPNISLTGFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(15012..15422)
                     /locus_tag="WHT44_000258"
     CDS             complement(15012..15422)
                     /locus_tag="WHT44_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ2532497.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(15436..15873)
                     /locus_tag="WHT44_000259"
     CDS             complement(15436..15873)
                     /locus_tag="WHT44_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ2532498.1"
                     /translation="MKISIFWLYNAFILERSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(16284..17582)
                     /locus_tag="WHT44_000260"
     CDS             complement(16284..17582)
                     /locus_tag="WHT44_000260"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ2532499.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGVGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMPGWRESTFGVKDYGA
                     LPENAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(17686..18837)
                     /locus_tag="WHT44_000261"
     CDS             complement(17686..18837)
                     /locus_tag="WHT44_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ2532500.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGAVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAHEAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            19172..20551
                     /gene="norM"
                     /locus_tag="WHT44_000262"
     CDS             19172..20551
                     /gene="norM"
                     /locus_tag="WHT44_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ2532501.1"
                     /translation="MLLDLNRFSFPVFLKEVRLLTTLALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYT
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            20595..21635
                     /gene="murB"
                     /locus_tag="WHT44_000263"
     CDS             20595..21635
                     /gene="murB"
                     /locus_tag="WHT44_000263"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ2532502.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADELRDIVRLLEFD
                     RDTVLWLGGGSNILLMQDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHT
                     VALGLSGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVELANADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPTLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            21647..22297
                     /locus_tag="WHT44_000264"
     CDS             21647..22297
                     /locus_tag="WHT44_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ2532503.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLAYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHRKKYDRKIGNGNP"
     gene            22352..23149
                     /locus_tag="WHT44_000265"
     CDS             22352..23149
                     /locus_tag="WHT44_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ2532504.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAVKRSLTSDDI
                     NLPIHLDTIDLNQDSAFQSANLARDTSFWRHHANKPVWFCLLPPSSLTHYADTVKQWA
                     ELARACNVQHLIFTSSTSVYGDTARECDEIALPDPQTESARQILAAEQHLLDSGVPNI
                     DILRLGGLYCAERHPVGRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKN
                     IIEPRHPTRREFYTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            23200..23766
                     /locus_tag="WHT44_000266"
     CDS             23200..23766
                     /locus_tag="WHT44_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ2532505.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            23786..24676
                     /locus_tag="WHT44_000267"
     CDS             23786..24676
                     /locus_tag="WHT44_000267"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ2532506.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            24701..25459
                     /locus_tag="WHT44_000268"
     CDS             24701..25459
                     /locus_tag="WHT44_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ2532507.1"
                     /translation="MNPKISSDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NHWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
BASE COUNT         6081 a         6484 c         6097 g         6800 t
ORIGIN      
        1 ccgtcatcaa aagcgaaatc gtaaatatgg tcgtcgtgga ttttgcgggt tttatcggta
       61 atgttttcag acggcctgtc cagatattcc gtcaaaaacc gcgcccattc gccgtctgaa
      121 aacgccacat cgttcagccg ctgcaactgc gcgcgcaggt tttccagcag cctgctttgg
      181 ctgttcaaat ccttgcggta ttcgtaaccc tgattctgca aatcggcgat taactccgct
      241 tccaaccggt tttccgattg gtagctgccc gactgttcga ttttttcata ttggtcgagc
      301 acgatgaaat tcggcgtttc agcgatgggt ttggtttcga ggttcatggc ttatctcttt
      361 gttcatcatc ttgaaaaaaa ttatagttat ccagcaagtg ttgcaaaagc aatttgacag
      421 tttcttgttc ttgtggtgtt ggctcggcaa atgcctcatt agataatgta gaatgacttg
      481 taaagttaat aatacgttga aaatagtttc gtctgctgtc ttcgggcaaa atatcagacc
      541 tttgcttata gcctaaaaaa ttagctgttt tttcatacag gtttctcagc aacataaaat
      601 gaaaccgttc tacctgctga ttctcgatag ctttttcaat aactcctttt aagtgaaggt
      661 gatatgaaaa acttttattg gaatcacctt gtttttcaag aatatcaaaa ctaccatctt
      721 cattttttaa cagaagataa cttttctttt ccttttctaa tttttttagt tcattgtata
      781 gaacgttgta aaataaaaca ttatgtgtag taatgataaa ctttaacctc ggtttgctaa
      841 gctttatcaa atcagccaaa tcaaccgctt gctgaatcag atgattttca tccaaagagc
      901 tgacagggtc atcaataaaa atatattcca aatcgtcaaa ctgatctgtg gaacgtcctt
      961 cttcgctatc ttctggaata ttcaattcag cgatgacttg tctgattaat acatagaaaa
     1021 tgctccaaat aaaattactt tcttcacctt tggaaatttt gatattttca atctgctcat
     1081 cattaccacg agcaaaataa aatgcaattt ctgaaaaatc atcattaaaa tcaggagtca
     1141 acttatcatc tgtataatat tgaaagtttt taataacagc tccatccagt ccattatccc
     1201 taagcaacca gtcggtaaaa ctattctttt gaatttttaa tcttggagct tcgttcgcta
     1261 acaaatcatt gtcccaaaag aataaatcct cagtaaatgc attatagtag agaaattttc
     1321 ttcttgttgg ctctccgtct ggctcttctg aactggttgg agcaattaaa ttcttaaact
     1381 ctctggacaa acgtgttttc cctgttccgt taaaagcata gattagctgg acttttttat
     1441 cgtttccttt tagttcctca gcaatttcgg ttaaactttt tcccatgata tttccttatc
     1501 aatatttttc agacggcatg atttaaagac cagctatgac ttttcaggca gcctttggga
     1561 agctcaacaa ctgcccgcgg taatattcgt attgtttccg gcgcagggca atttcgtacg
     1621 gtaggccctc gctgatggag tgggtcaggg tgtcgaattt gtcgaggatg gcgacgattt
     1681 tttcctgttc ggggagtgga gggatgggga ttgaaatatc ttttatcatt ggtttcgtta
     1741 gctgtggaat accgctttca ggaactttat aatttgattc aatacttttc ataaagtagt
     1801 aggcatattt taggtttaat tcaatttttg gtgttaacac taacaggcgt actattggga
     1861 aaaaaggttt attctgaaag ctagcccaac ctatagttcc tctagctgat atagttaagc
     1921 taggttgatt gattttagca acattagtcc agccatatag tgccttactt cctataccat
     1981 tagataaaat ggggatacaa aattcttccg tttccacttc agagaaagcg tcttttggta
     2041 cgtcgcctcc agcaaaaata ttaaatacct cccccaacgt cttccaaacc acatctttca
     2101 gacggccttt atagccatca gctatccccc ccgatttgat tgttaaaatc taaaagaaag
     2161 tcgcggtaat accggtattg gcgtttgcgc agggttaatt ccgcttccag cgtagcttac
     2221 agctctgtga atttgtcaag tatttttaca attttttgtt gggtttccag gggtgggatg
     2281 gggatcgaaa tattttcaac tactaatcta tcaatagtag gaactccacc aactctcttt
     2341 tgggatttta tatatccttc tcttgttttt aaaaagtaga atataaactt atcatcaaga
     2401 tagtttggtg tttgaatcac aaaagtacca tcagatgacc agaaatctac gccactccat
     2461 ataacttcac caatacttcc cgtattaata atcatcgttt gattagctct acgattaaac
     2521 tggtcaaacc aaccaagagg aatcaatccg ccatgaaaaa ccggatattt cccaccttca
     2581 attagctctt tctttgtcaa acgttttcct cttaatactt tcgccacttc ccccaacggc
     2641 ttccactcca ccggtgccgt ctgaatcatc tcaatcaatt ttttcgcttt gttttgcata
     2701 tccatggttg ttttccgttt ctttcccgtt gcggcgggat gtggggcagt tgcggggtgc
     2761 ttcggctcac tctgtccgtt tgccgttatt tctgcgggct gaagtccgtc ctgctttcag
     2821 gctgcctgca atggtgtgca ggctgctttt tcaggtagcc tgaatggtag ttattttgtg
     2881 cggtcttttt tctgtttgat ctgttgcacg gctgtttttt ccaacgcttt aacgctttcc
     2941 agataggctt gctccaccgg cgacaggatg cgcgcttcat aggcgcggta ttcgcgttcg
     3001 gctttctgct ctgcctgttt gcggctgatg ctgcctgcac cctgtaatgt gccttgtccg
     3061 tacagcccgg aaaatttgtc caattcggct atccagtcgc gcatatacat ggggctttgc
     3121 tcctgcgctt tgatttccgc taggtcgaag aaggcggaaa ccagacggtt caggcggaac
     3181 agttcgtctt cggtcagata gtttttggcg attttggctt cattcagcgt ggggattgcg
     3241 ccttgaaagg tggtcagccc cataaagtct ttgctgctgt cggcacggct gtatatcagc
     3301 tcagctgcgg tttgccggct ggcggcatag tgcagtttgt tttgaacggc ggcaaaaaag
     3361 gtttggcttt cgctgctttt ggggttgtag tcttggctgg tggcatataa atcaagcact
     3421 tgccggtata gggctttttc gctgctgcgg atgtcgcgaa tgcggttgag cagttctttc
     3481 caatagtcgc cgccgcctgt gcctttcagg cgttcgtcgt ctatggcaaa gcctttggtc
     3541 agatattcgt ccagccgttc ggttgcccat tggcggaatt ggatgccccg cgcggaacgg
     3601 acgcggtagc cgacggcaat aatcatgggc agggaataat gggcgatttt gcggtttacc
     3661 ttgcgcccgt tttcattttg aactgtcaac tggaagttga cagttgcctt ctcttccaat
     3721 tcttgctctg caagaatggt tttaatgtgt ttgctgatat tttgtttggt ggtttggtac
     3781 agttctgcca tgtccgcctg cgtcagccaa agctgtccgc caaattcctg caaggcaaat
     3841 tgggcagtgc cgtcgttggc ggtgtatagg atgatgctca tgcttcaatc tctgcaatca
     3901 cttcgtcaat ttcacgccgc agccgttcga ttttggcgac ggtttcgccg atttcggcgt
     3961 tgagctgttt gatgtcgata atttcgcgtg tgtcttcggc ttcgacatag ctgctgacgg
     4021 cgaggttgta gccgttgtct ttgacggttt gctgggcagc gttttgggcg atatgcggca
     4081 catcggcttt atcggcgaag agtttgacga tttcagcaat gtgttcttcg attaagacgt
     4141 tgttgttggt ttctttttta aagaagccgc ttgcgtcgat gaattggatg tcggtattgt
     4201 ctttgtgttt ggacaaaacc aggatattga cggcgatgcc ggtgccgtaa aagagattgg
     4261 gcgcaagggc aatcacggtt tccacgtagt tgccctccac cagatattgg cggattttct
     4321 gttctgcgcc gccgcgatag aaaatgccgg ggaatgagac gatggcggcg cggcctctgc
     4381 cggaaaggta gttcagtgcg tgcaggatga aggcaaaatc ggctttggat ttcggggcaa
     4441 gtacgcctgc gggggcaaag cggtcgtcgt tgattaaggt ggggtcgtcg ctgcctatcc
     4501 agttgatgga ataaggcgga ttggaaacga tggcatcaaa gggtttgctg tctttgagct
     4561 ttgggttggt cagtgtgtcg cccaattcga tgtggaattg gttgtaattg acgttgtgca
     4621 ggaacatgtt catgcgggcg aggttgtagg tggtgtggtt gatttcctgc ccgaagaagc
     4681 cttcttcgat gatgtgctcg tcaaactgtt ttttcgcctg caagagcaga ctgcccgagc
     4741 cgcaagctgg gtcgtagatt ttgttgactt tctcctgtcc gtgcaccgcc agccgcgcaa
     4801 tcagcttgga tacgctttgc ggggtgaaaa attcgccgcc ggatttgcct gcgttggcag
     4861 cgtagttgga aatcaggtat tcgtaggcat cgccgaaaag gtcgatgtgg tggttttcaa
     4921 aattgccgaa atcgagttcc gccacgcctt tgaggacggc ggcaaggcgt ttgttcttgt
     4981 cggcaacagt gctgccgagc cggctgctgg tggtgtcgaa gtcgtcaaac aggcctttga
     5041 tgtcctgttc ggacggatag ccggaggcgg agctttcaat cgcggtaaaa atttctttca
     5101 gcttggtgtt gagctcttcg ttttgatggg cttcggcggc aatattgcaa aaaagctggc
     5161 cggggtagat gaaatagcct ttaactttga cggcatcgtc tttgatttcg ggcgtgatga
     5221 tgctgtccgg catagcggcg taatcaatac tgctgtcgcc tgcctgcata tagtcggtga
     5281 agttttcgct gataaagcgg tagaaaagtg tgccgagaac gtattgttta aagtcccagc
     5341 catccaccgc gccgcgtact tcgtcggcaa ttttccaaat ttggcggtgc agttgggcgc
     5401 gttgttgcat ttcggtcatc atcgaaatcc atataaaagt taaacaaatc aaaatcgcct
     5461 gatattttca gacgattttt ttacgggcat tcaaattaag ccaaattttc cagcatccat
     5521 ttgacgtagc ggtcgacgcc tgatttgaca tcgaaaaatt cttccttata tccggcttcg
     5581 cgcaatttgg tgatgtcggc ttgggtgaag ctttggtatt tgcctttgag cgcgtcgggg
     5641 aagggaatgt agcggataag ttcttcttct accaactctt tcaagctcat ttcaggtttg
     5701 ccttcggcgg cgcggcatgc gttgacggtg gcggcggcga gttcgttgaa ctgttggctg
     5761 cggccggtac cgaggttgta gatgccggaa agttcgggat ggtcgaagaa gtagaggttg
     5821 actttggcaa cgtcttcgac gctgacgaag tcgcgggttt gttcgccgtt gccgtagccg
     5881 tcgttactgc cgaacaggtt gacgtaaccg tgttcgcggt attggtggaa gtggtggaag
     5941 gcgacggatg ccatgcggcc tttgtgttgt tcgtgttgtc cgtaaacatt gaagtagcgg
     6001 aagccgacga cttgggcggt gagaccttct ttcatgcggc gacgcaatac ttggtcgaac
     6061 aggaatttgg agtagccgta cacgttgagc ggtttttcga gttcgcgctc ttcgcggaag
     6121 atttctcctt tgccgtaaac cgccgcactg gaggcataaa ggaaggggat gcgttcgtcc
     6181 tgacaccagt ccagcaaatc cagcgtgtac tggtagttgt tgtccatcat atacaaaccg
     6241 tcgtggttca tcgtatcgga acacgcgcct tgatggaaaa cggcttcgat gttttgataa
     6301 ggtaaaatgt gttccctcac ttggcggatg aattcgtgtt tgtcgagata atgggcgatt
     6361 tcgcactcgg caaggttttt gaatttttcg cctttgctca aattatcgac ggcaacaatg
     6421 tcagtaatac cgcgttgatt aagtgctttg acgatgttgc tgccgataaa gccggccgcg
     6481 cctgttacga tgatggtcat attcggtttc ctttgttttg agtaaaatta ataaacttag
     6541 ttacaagaaa tcatcgatat tgtcctgaaa cacaaatttg cgatgatatt ccaaatattt
     6601 ttctcttcct tcgggatata ttctcatctt aattttttgc atttggattg atattaagtt
     6661 tttcccatgt gtaacggctt agtcgagtac cgcaaatcaa acgaccatca tccaagaaag
     6721 taatataacc ttgatcgaac aaccaatcat aagtcggcgt taaagctaac ccatttaaat
     6781 aatctaatgc ctctttctca tttttctcag taatacaaac catataaggt ttaatatgac
     6841 tagcccttaa caaaatctca tctgtaatta atgtaaatgg acattgcgac atatgattta
     6901 taacatcctt gcgatatttc tgctgccctt ttctactttt ctgtttaatg atttcttgct
     6961 ctacgaattg attaaattct gaagccggta tttctaattt ttctcttgat aagagttgcg
     7021 ggtgcacgat agagcgaaat tgataatcca aaaatattcg gaaataaaat aatggttctg
     7081 aatcttctat atctttgaca ggcaatagtt taagaattga taaataacta attttgggca
     7141 atacaatctt acgccaaatt gaccatatat catcctccga acggatataa cccctacggt
     7201 aacctttaga attttccaaa tggtctgata catcatggat tgaaaagaaa atattttctg
     7261 taggcagatt attaatactt aaaacgtaat catcataaaa ccttgaaata tcatgatgat
     7321 atttctgttc ttgtttgtga tattcaactt ttgcatcaac caaataacgc tgtaaatttt
     7381 tcttataaaa aaaacaattt ttctgaatga aaggatattc taccctcctt ccattttttg
     7441 tctcagtaat cccccaattt tcatactcaa aaaatgtact tagttttcga ccactatcat
     7501 caatataact acctacatat ttgcgtgctt ccccattacc accaaaaaca gataacttgt
     7561 tattcctgtg aataaaacta tcttccgcac gtaaattttg aatactatcg acaataaaat
     7621 actctatgtt ttcaatagta attctttgaa aatccggcat tttactctcc ataaagcgta
     7681 atatccattt gttttaatat agcgattaat acatcaacaa caatactgtt tcccgcttgg
     7741 cgatacattt gagtatcact aactaaaatt ttaaaatcat ctctaaaacc cattagccgc
     7801 aaacattccc taggtgttaa tttacgaata cgtccacgat tatgagtaac ataattatca
     7861 accccggcac gatgcatttt atgcatagtt tgcaataatg ggcgagctac aggcaaatca
     7921 gtttccgtac tggttttaaa attttttgtt cctcctgcca aaacataatt tttgattttt
     7981 tcagaaagat aatatttttc ctcaacgtta tttacatcaa aaataaagtc atcaaattca
     8041 gattcgcggg ctgcctgaaa aataaaatca ccatgccaat taaattgttg atttgctttt
     8101 tggcataagg caatttctcc attaatctgt gtataacgtt tttgtctatt tttagaactg
     8161 gtaacaaatt tcgcaccctt ttcacgtaaa aaatatttgc tatcagtata gtcctccaaa
     8221 aaatcttgca tagtatgttc taattcaatc ttttcaggaa actgaaaacc atttatagga
     8281 ggggtatgaa agccaacaac aaaaatacgc tcacgatgtt gaggaatccc ataatcctta
     8341 ctattcatta tttggaaata taagtcataa ccaagtgaat aaaaaacact ttttacaact
     8401 ttccaagttt ttccattatc atgattaagc aagcccttta cattttcata aataaatatc
     8461 tttggttgga cttcatccac aacgcgagca aattcataga ataatgttcc tcgtgtatct
     8521 tctaatcctg cacgtttgcc aaccatggaa aatgcttggc aaggactgcc tccaactaaa
     8581 atatcaactt gatttctaaa ctttctcgca tcaaattgag taatgtcgtt atgccaaaaa
     8641 tcttcattta atttatagtt tccaagataa ctttttttaa cgtatggatc aatatctcct
     8701 gaaaaaacaa tggtatggtt taaatttaat cggtggaatg cctgttcaac cgcaccaatc
     8761 ccgctgaata cggttgctaa tctaatatgt gaatcaggtt taagaaaagt tttagatttc
     8821 caaccttgtt gactgggaaa gagcaaagtt ttttgtaatc gagtatcgtg tgtctgtgcc
     8881 attgtcgaaa tagtcatact tatatcgttc tgtttatctt atcaatatga aaactacatt
     8941 gttgattgcc ctgacaatgc cttgatcaat tcggcaaacg agcaaaccgc cgtaccgagt
     9001 ttcgccacga caaccccggc cgcagtattg gcaaggtaca tggcttcggg catggtgcag
     9061 cctgccgcca aacccaagcc cattccggca atgacggtgt cgcccgcacc ggatacgtcg
     9121 taaacttctt gggcgcgggt gggctggtaa atcggttcgc cttcgctgaa caaggtcatg
     9181 ccttcttcgc ttcgggtcag taaaacggcg gtcaggtcga ggtggcggcg caggttttgc
     9241 gctttttcgg tcagctcgct ttcgtttttc caactgccga ccacttcttt caattcggcg
     9301 cggttaggcg taatcagagt tgcaccgaca tatttttcgt aatcgtcgcc tttggggtcg
     9361 attaagacgg ttttgccggc gtgtttcgcc caatcgatca tatcggagat atgcgacagg
     9421 ccgcctttgc cgtagtctga aaaaatgatt gcgtcgtatt cgggcaagat ttcgcggtat
     9481 ttctgcttga tttgttccaa cacttcgcag ttgggatgtt cttcaaaatc aagacggata
     9541 agctgctggt tgcgggcgac gacgcgcagt ttgacggtgg tggcgatttg tttgtcgcgc
     9601 atcagatagg aggcgacgcc gtcctgcacc atcagcgcat cgagcgcgtc ggcggcttcg
     9661 tcgttgccgg ttacggacaa cagccctgcc ctgccgccca acgaagcgat gttgcgcgcg
     9721 acatttgccg ctccgcccgc gcgttggtcg attcgtccga ttttcgccac cggcacgggg
     9781 gcttcgggcg aaatacggga cacatcgccg aaccaatagc ggtcgagcat cacgtcgccg
     9841 acaaccagga ctttggcttg cgcgaaacgg gatttgaggg tttcttgttg gaacttggcg
     9901 gacatattgc ctccgttgta ttttttcaga cggcctcagc ctgtaaggcc gtctgaaaat
     9961 cagtttgctt caaggtttgc cacgcggttc acttcgcgca atacggctac cggatcggca
    10021 gcttgggtta caggacgacc cattaccaaa taagtcgaac cggcagccaa ggcttcggcc
    10081 ggtgtcatga tgcggcgctg gtcatcatta ttgccggcaa cgtccaagcg gatgccgggc
    10141 gtgaccaaga caaaatcctg tcccaattcg cggcgcagcg gcgcggcttc ttgggcggaa
    10201 cagaccacgc cgtccaagcc cgaactttgc gccagttttg ccaagcggat gacttgttct
    10261 tcaggggcgg tgttcaaacc gatttccgcc aaatcacttt gttccatgct ggtcaacacg
    10321 gttacgccga ttaagagcgg cttcgtgccg tatccggcaa cggcttctgc tgcggcttcc
    10381 atcatacggc ggccgcccga tgcgtgcata tcgaccatcc aaacgcccat atcggcagcg
    10441 actttgcagg cttgcgcgac ggtgtgggga atatcgtggt atttcaaatc gaggaaaagt
    10501 ttgaaacctt gattgattaa gctttctgcc aaattgcgtc ccgtcgcggt aaacagctct
    10561 ttgccgattt tgatttgaca caatgtcggg tcaaggttgc ggacgaatcc gagcgtgtct
    10621 ttttcgttgg aaaaatcaag ggcgacgata acgggggtgc gttgttgcgg agtttggaag
    10681 tcggagatta aaggattcat ggtgttttcg tttcgggttt tcagacgacc tttatcgtct
    10741 ttttcgcagt atagtggatt aactttaaac cggtacggcg ttgtctcgcc ttagttcaaa
    10801 gagaacgctt ctctaaggtg ctgaagcaca aagtgaatcg gttccgtact atctgtactg
    10861 cctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctataacaac gtaaattcaa
    10921 accgtaggtc gggcattgat gtccgaccta cgcggactca agcctccgac aaactgtttc
    10981 ggataaattt gaaccggtac atcagccgtc attcccgcgc aggcgggaat ccattgataa
    11041 aactcagttc atcagattta aacagcgatt gcccaaattc agaaatggat tcccgcctgc
    11101 gcgggaatga cgccaaatga ttatttttcg gttgcaacac gctgtttatc aaaaacaggt
    11161 cgtctgaaaa cctgattttc tgttttcaga gtgcgaagcc tgcccgtggg tacgaccttt
    11221 tcgatttatt tgcccaatgc gcccaatact tcggctttca cggcttctac tgcttgggtg
    11281 ccgtcaactt tgatgtattt aggcgcgtgt tcgccttcca gtttgctgta aaaatcgacc
    11341 aaaacttcgg tttgctcgtg gtaaacggca aggcgttttt tcacggtttc ttctttgtcg
    11401 tcgtcgcgct gaatcaaatc ttcgccggtt acgtcgtctt tgccttcaac tttgggcggg
    11461 ttgtaggtaa cgtggtaagt acggccggaa gccaaatgca cgcggcggcc gctcatgcgg
    11521 tcgacaatca cgctgtcagg cacatcgatt tcaacgactg catccaaatc cacgcctgct
    11581 tcaaccatcg cttcggcttg tgccaatgtg cgcgggaaac cgtcaaacaa gaaaccgttt
    11641 ttgcagtcgt cttgcgcgat gcgttctttg accatgccga taatgatgtc gtcgcgcacc
    11701 aagccgcctt cgtcaatgat ttttttcgct tccaaaccca agggcgtgcc tgccttaatc
    11761 gcggcacgga gcatgtcgcc ggtagagatt tgcggaatgc cgaacgctgc ggtgatgaat
    11821 tgcgcctgag tgcctttgcc cgcgcccggc gcgcctaaaa gtaatgcttt catgaagtga
    11881 tcctttgaaa tgtgtttatt ggaatcaaac agatggttta cgattctaca cggcttggca
    11941 caatagttca aaaaccaact gcacaaactg ccatttgcaa gcaaatgttt tgcatacctt
    12001 tgccgagtct gatattcaca agccggcaac tttttgggac aatcccccaa aatttcaaaa
    12061 catcacaccc taccaaaaag gaacatccgt gaaacgaatt tttctgtttt tggctaccaa
    12121 tatcgctgtt ttggtcgtaa tcaacattgt tttggcggtt ttgggcatca acagccgggg
    12181 cggcacgggc agcctgttgg cgtattccgc cgtcgtcggc ttcactggtt cgattatttc
    12241 gctgctgatg tccaaattta tcgccaaaca atcggtcggc gcggaagtta tcgacacgcc
    12301 gcgcaccgaa gaagaagcct ggcttttgaa cactgtcgaa gcccaagcgc ggcaatggaa
    12361 cctgaaaacg cccgaagtcg ccatctacca ctcccccgaa cccaatgcct ttgccacggg
    12421 cgcatcgaga aacagctccc tgatcgccgt cagcaccggt ttgctcgacc atatgacgcg
    12481 tgacgaagtg gaagccgtat tggcgcacga aatggcacac gtcggcaacg gcgatatggt
    12541 tacgctgacg ctgattcaag gcgtggtcaa tacctttgtc gtgttcctgt cgcgcattat
    12601 tgccaacctg attgcccgaa acaacgacgg cagccagtcc cagggaactt atttcctggt
    12661 cagcatggta ttccaaatcc tgttcggctt ccttgccagc ttaattgtca tgtggttcag
    12721 ccgacaacgc gaataccgcg ccgatgcggg cgcggcaaaa ctggtcggcg cgccgaaaat
    12781 gatttccgcc ctgcaaaggc tcaaaggcaa cccggtcgat ttgcccgaag aaatgaacgc
    12841 aatgggcatc gccggagata cgcgcgactc cctgctcagc acccaccctt cgctggacaa
    12901 ccgtatcgcc cgcctcaaat cgctttaaac cgatttgaaa cggcaaaaac cgtacgcgca
    12961 agcagtacgg ttttttgtat atgctgtctg aaaacatcag gtttccatac cgacaccaac
    13021 cgtatccatt tccgcaccgg atacgccgca ttgatatacc ctgataaacc gtatccggac
    13081 agaaaatata gtggattaac aaaaaccagt acggcgttgc ctcgccttag ctcaaagaaa
    13141 acgattctct aaggtgctga agcaccaagt gaatcggttc cgtactattt atactgtctg
    13201 cggcttcgtc gccttgtcct gatttttgtt aatcccctat atcagataaa acagcggtaa
    13261 aattgccgtc tgaaaccaac tctgcgccat acgggacacg ctctgcacgt ctatttgcct
    13321 ccgatatatc ctccctactg taggaacgtc tgccgatttg cacgaaatcg gcacctctag
    13381 cacccttcag gatggcggac gttatccgtt cttgggattc tgcaagacgg tcgacggcat
    13441 tatacagacg ctcggtctga tacccttccg aagcaacctg cttcaggtat tccaaataac
    13501 cgtaacccaa cgcccctccg acaagcaggg tcgtcaacgc taccagcgtt gccgaacgga
    13561 ttttaggggg catatcttga agcatatctc taccgaaccg aaccaaagtc tctttaaagt
    13621 cggactctac acgcgtagaa ccctcggaaa tcttgaagtt caactcatac tgcttccgtt
    13681 tgtccgctgt caaccgcttg aggttgtcat ccccgtattg gacggttgac acaaccctgt
    13741 acagactgtc ctgtatttcg accagcgtct gtgcaaagtt ggcggtcata actggattcc
    13801 caacccttgc cgccgattct gatactcaaa acggtatcca ggcgttccag cagggcttcg
    13861 ggagacagca gtgcgtattc ttcaatattt tccataggtt gattatagca gaaccttata
    13921 cctgttacag acaaggttgt cgggatttga acgtccgtcg agcttggttc gtatagagcc
    13981 gtagggggtg tttagggctt tggcggcagc ttgtacgctg gggaacgtga gtgtttcgcc
    14041 tgtggcggtg tcgaggatgg agacaggttt ttggttttgg ggaggcgtat tttccttcat
    14101 tttaagtttc ccgcactccg gacacccata ctttgacttt atagctgtgt cgtaatacaa
    14161 aagttggaca accccgtgcg taggacatct aacactggaa ggtttataaa tgccactgaa
    14221 ctccaacaat tctaaatgtg gaaatttctc ttgaaagcgt gatttggcgg tatcgaaagt
    14281 cattcgctta cccataattt gtctccatag gttatgtgca aatggattac acaaccaaaa
    14341 tatagaaatt ttcaaatttt ttgagaccta cctgaaatcc tgcgcaagca caatgtaaag
    14401 aaaaatggta tacgtcttta ctaaatcttt acataaatta actctaaccg ggatttgaac
    14461 gtccgtcgag cttggttcgt aggatttagg gtgttgtgaa ccttacctat cgtacccaag
    14521 cactttgtac ctgtttgaaa taaggttgtc gggcgacgtt cgacccttga gcctgtgatt
    14581 gatttgttgg aaatgtacac ctaaagcagc acctgcggca gtaacgcttg ggaacgtgag
    14641 tgtttcgttg gtcgttgtgt cgaggatgag gaggggcttc cctacatttg gaggtgtttg
    14701 ggtttttgac atcttagcac actcaggaca cccgtacttt gactttataa gggcattaaa
    14761 tgtcgaataa cgaacgttcc cgtgcatagg acaattaacg gttactgggt aattcacacc
    14821 gtcaaagccg gttagagaga tattagggaa tatatcagta agttcagaag ctgcttggtt
    14881 gaaagatttg gccatttgag actccaaaat tgattgtcaa atggatttaa tagcaaattt
    14941 ataaaaatgt caaatttttc gagaagcacc tgatagtggc gaggggtata cactgttaaa
    15001 aagtgtatac gtcatttagt gcagatgtga accttatacc tccctgcaag taaagtctct
    15061 gtgtgaaccc gaccttttat acggtcagtg atagctgctg ggtttatacc caatgacctt
    15121 gcagcagatg ctcttgaggg gaatgtaagt gtttcgccag ttacagtatc ctccaatact
    15181 gtatttttag ataatttatc tattgtatct tctggaattt tgtgggaatt gacaccataa
    15241 gatgcacatt ttggacaccc acttttagag tccaacatac ttttgaatgt agagcaagta
    15301 accctcccgt gtataggaca gacgattacc gtcgggtaac gaacgccatt gaactccaca
    15361 agattcaaat gtggaaattt agaggttagc ttagaaactg cttgagtaaa tgtaagtgcc
    15421 atagtggttg tgaacttact ttaccaacct ataacgatac ctaatcaatc ggtcaaacgg
    15481 agtgcgtccg cttaaacgta cagctaaagt gctgttggag caaccgataa atttactggc
    15541 agccagtgta cttgcaaact cgtagatttc ccctgtctct gtgtcctcca acctaatggc
    15601 aacggcgttt ggagagggct tttcgggatt tcgagtacat tcaggacatc ctgttgagga
    15661 tttcatggca gcttgtagat tcccgtaaac aaccgaccca tgcaaagggc attggattgt
    15721 taccggttcg tagtttttag tgaacttcaa aaccgtctta tcaggaaatt tactggaaaa
    15781 ccttaactga acatcttgta gcgagagtga ttttggcatt atagaccttt ctaaaatgaa
    15841 agcattatac aaccaaaata tagaaatttt caaattttct ctgaacgcac tggtatccga
    15901 tggagctaaa tgtaaagaaa agggtatatg tccttactaa atatttacac attttaactg
    15961 tatagtgttt gacaaaaaac aggcataggc gcataatgca cccgtagtta gatagatagt
    16021 aaggagataa ccaatgactt agcacctgcc cccctctctc tcgcgcaccc cgcccctcgc
    16081 ttgcggctaa tgggctttgc gcgacatata cactttcatc tcctgcatgg cttaaccgcc
    16141 tatgcaatag tacacacatc aacaaatcag gctggcacgt cttggcattg gctgacgtac
    16201 cattgtagcc cgaaatgttg ataccctact atttatgacc aacggcggat tagggcttca
    16261 gacggcaatt ttactgcaaa accttatgcg aacggatgat gcagcacaat cgtttcttct
    16321 cggtcggggc cggtggagac gatggcgacc ggcgcgccgc agacttcttc aatccgtttc
    16381 aaatatgctt tggcgttttc gggcaatgcg ccgtagtcct tcacgccgaa agtggattcg
    16441 cgccagccgg gcatggtttc gtaaatcggc ttgcaggttt ccaccgcatc ggaaccgcaa
    16501 ggcaggatgt cggttttgcc gccgtcgggc aattcatagc cgacgcagat attgatggtt
    16561 tcaacgccgt ccattacatc gagtttagta atacacatac cggaaatgcc gttgatttgg
    16621 atggagcgtt tcagggcggc ggcatcaaac cagccgcagc ggcgtgcgcg tccggttacc
    16681 gaaccgaatt cgtgtccgcg ttctgccaaa cctacgccta cttcgtcgaa caattcggtc
    16741 gggaacgggc ccgaaccgac gcgcgtggta taggctttga cgatgcccaa aacataatcc
    16801 agcatttgag gacctacgcc cgcgcctgcc gaagctgcgc ccgccagaca gttggacgag
    16861 gtaacgaagg gataagtgcc gtagtcgatg tccaacaacg caccttgcgc gccttcaaac
    16921 agcagttttt cgccgttttt gtttttctcg ttcaacacgc gggacacgtc ggtaatcatc
    16981 ggcgcaatgc gcggcgcgac tttttcgata accgccatta cgtcttccgc tttaaccggc
    17041 tcggcattgt gcagatgttg cagttggaca ttgtaatagg caaggacggc atccagtttt
    17101 tcacgcagtt tttcaggatg cagcaaatcg gcggcgcgaa tggcgcggcg tgccactttg
    17161 tcttcgtagg cagggccgat gccgcggccg gtcgtgccga ttttgccttt gccgcgcgat
    17221 gcttcgcggg cttggtcgag cgcgatgtgg taaggcagga tcagcgggca ggtcggcgcg
    17281 attttcagac ggccttcgac gtttttcacg cctgccgcgt tcaactcgtc gatttcgccc
    17341 aacagggctt cgggggagac gacaacgccc gaaccgatga agcagtccaa actttcatgc
    17401 aggatgccgc tcggaatcag gcgcaaaatg gtttttttgc cgccgacaac caaggtatgg
    17461 cccgcattgt ggccgccttg gaagcgcacc acgccgccgg cttcttccgc cagccagtca
    17521 acgattttac ctttaccctc gtcgccccac tgtgcgccga ttactacaac atttttagcc
    17581 atagccatat aacctatcga tattaaaaat tattgcgaaa atccccaaac cggtgcggct
    17641 ttgcgccccg ccacgatttg cctttcatcc gccatgaacg ggtatttagc gtttcacgac
    17701 ctgccaaacg ccgtccgtct ttttcagacg gcctgcaagc tcttccgaaa cattgtgtcc
    17761 gataccgtaa tcgattacga cacactgccc ttgttcacgc aaggcttcga ccgcttcgtg
    17821 cgccgcttcg gcatcttccg catcgaccaa cacggcgggc tgccgttcga tggcgggcaa
    17881 acgcccgata aagctgcgca agtcgaaact gaatcccgtt gccgggcgcg ccctaccgaa
    17941 atatccgccc aatccgtcat aacgcccgcc gcgcgcgacc gcgtcgtgga aatcggcggc
    18001 ataggcggca tacagcaagc ccgtgtggta attgtcgaca cgcagctcgg acaagtcgat
    18061 atggatttca caatcgggga atgcgtcgca caccgcctgc aattcgccca acgcgccgcc
    18121 gaccgccgac aaatccggca accgtccgcg cgcgtcggac aacacttcac gcccgccgta
    18181 caggcgcggc aacagcgaga atgcttttgc ccacatgccg tccagcttcc aagccttgac
    18241 ctgcgcttcg accgccccgg tatctttatc ctgcatcaag gcaagcagcg ttgcggactg
    18301 ccccgcatcc aaatgtgccg catcggacaa ggcgcgaaat atgccgatat gccccagcga
    18361 aagcagcact ttgcccatat cggcaatttt catgcttttc agcatcaggt ctatcagctc
    18421 gatgtcgcca cggatgtcag caaaaccgta catttctgcc cctgcctgca agggttcgcg
    18481 catattcagc agaccgtcgg gctgcgcgtg caacaccgga ccggcataac acaaccggtt
    18541 aatcccttgg ttggcggata aaagatgggc atcgatacgc gccacctgcg gcgtgatgtc
    18601 ggcgcgtatg cccaactgcc tgccgctgag cctgtccgtt accaaaatgg ttttcaggga
    18661 aagccccgca tcgatatgcg tcagcaggga atgtgcgtac tccatcagcg gaggctgtac
    18721 cagttcataa ccgtgtacgc ggaacagtgc caacaactgc tccctcgcgc tttcaagctg
    18781 ccgcgcgttc gtgggcagta cgtcggcgat atgttccgga agctgccatg tctgcatgag
    18841 aatctgcctt ctattttatc ctgtaaacac aaaagactgc ccgatccgca atcagacggc
    18901 atctttgccg caacgcatca cgccgctgtc aaaacggcgg tgcttctttc cgattcagaa
    18961 tccgaacagc ccctgtccga cggctcaatg ccgtcaagtc gttaaaacca aactttacca
    19021 taaaatacac acaatctgaa tatactttta taccctgtat ccgccgtctg acggcacggc
    19081 atttttttac gatataatgt gcaccattat ttctcatcac ttcctgctgc cgttttctgc
    19141 ttcagacggc atttttgttg attgaacact tatgctgctc gacctcaacc gcttttcctt
    19201 tcccgtcttc ctgaaagaag tccgcctgct gaccactctt gccctgccca tgctgttggc
    19261 gcaggtcgcg caggtgggca tcggttttgt cgatactgtg atggcgggcg gtgcgggcaa
    19321 ggaagacttg gcggcggtgg ctttgggcag cagcgcgttt gccacggttt atattacctt
    19381 tatgggcatt atggcggcgc tgaacccgat gattgcccag ctttacggcg cgggtaaaac
    19441 cgacgaagtg ggcgaaacgg ggcggcaggg gatttggttc gggctgtttt tgggcgtgtt
    19501 cggcatggtc ttgatgtggg cggcgattac gccgttccgc aactggctga ccttgagcga
    19561 ttatgtggaa ggcacgatgg cgcagtatat gttgttcacc agcttggcga tgccggcggc
    19621 aatggtacac cgcgcgctgc acgcctacac ttccagcctg aaccgcccgc gcctgattat
    19681 gttggtcagc tttgcggcgt ttgtgttgaa cgtgccgctg aactatattt tcgtttacgg
    19741 caaattcggt atgcccgctt tgggcggcgc aggctgcgga ctggcgacga tggcggtgtt
    19801 ttggttcagc gcgctggcat tgtggattta tatcgccaag gaaaatttct tccgcccatt
    19861 cggactgacg gcgaaattcg gcaaaccgga ttgggcggtg ttcaaacaga tttggaaaat
    19921 cggcgcaccc atcgggctgt cttatttttt ggaagccagc gcgttttcgt ttatcgtgtt
    19981 tttgattgcg cctttcggcg aggattatgt ggcggcgcag caggtcggca tcagtttgtc
    20041 ggggattctc tatatgattc cgcaaagcgt cggctcggcg gggacggtgc gcatcggctt
    20101 ttcgcttggg cggcgcgaat tttcgcgggc gcgttatatt tcgggcgtgt cactggtgtt
    20161 aggatggatg ctcgccgtga ttaccgtgct ttccttggta ttattccgtt cgccgctggt
    20221 aagtatgtac aacaatgatc cggcggtttt aagcatcgcc gccaccgtct tactgttcgc
    20281 cggcttgttc caaccggcag acttcaccca atgtatcgcc tcctacgcct tgcgcggcta
    20341 caaagttaca aaggtgccga tgttcatcca cgccgccgcc ttttggggct gcggcctgct
    20401 gccgggctat ctgctcgcct accgtttcga tatgggcatt tacggcttct ggacggcatt
    20461 gattgcctcg ctcaccatcg ccgccatcgc cttggtgtgg tgcttggaat tgtgcagtag
    20521 ggagatggtc agatcgcata aggccgtctg aaaacgcagt acacttcaat tcaaatacag
    20581 ttaaagttca aaccatgcaa cccatccgat accgaaccga ccttaccccc tacaacacct
    20641 tcggtcttcg cgcccaagcc cgggccttta tcgcgctcga acatgccgac gagttgcgcg
    20701 acatcgtccg actgctggag ttcgaccgcg atactgtttt atggctgggc ggcggcagca
    20761 acatcctttt gatgcaggat tacgccggac tggtcgtaca catggaaaac aaaggcatac
    20821 gcgagattgc gcgttcagac ggcatggttc tgattgaagc gcaggcgggc gaaatttggc
    20881 acgattttgt cctgcacacc gttgcgctgg gtttgagcgg tttggaaaac ctgagcctga
    20941 ttccgggtac ggtcggcgca tcgcccgtgc agaacatcgg cgcatacggc gtggaggcga
    21001 aagacgtgat tcacagcgtg cgctgctttg atttggatac ggagaccttt gtcgagcttg
    21061 ccaatgccga ctgccgcttc gcctaccgcg aaagcctgtt caagcaggag ggtaaagggc
    21121 gttatgtgat tgtttcggtc gtatttgcat taaaaacgca ttttgtgccg actttgggtt
    21181 acggcgattt ggcggccgcc gttgccgaac tgagcgcggg cagggtcccg acggcgaaag
    21241 atgtttccga tgcagtgtgt gcaatccgca acagtaaact tcctaatcct aacgtgcttg
    21301 gcaatgtcgg cagtttcttt aaaaaccccg tcgtcagcgc agaaaaagcc gccaccttgt
    21361 tgcagcggca tcctgatatg ccgcgctatc cgcagcccga cggttcggtc aaactcgccg
    21421 ccggctggct gatcgaccaa tgccgtctga aaggcttcca aatcggcggc gcggcggtac
    21481 atgacaggca ggctttggtc ttagtgaaca aaaacaacgc ctcggcaaac gatgtccggc
    21541 agttggcgca acacatcaaa tttacagtat ttgctcggtt tcaggtagaa ttgcacgccg
    21601 aacctaattg gctgcctgct tcgttcagcc tgtaaatcca aggagtatgc ccgtgacccg
    21661 ccccgccaaa atcaatactt acacacgcat catcgacgcc agccttgcgc ttttcaacga
    21721 ggaaggcgag cgcaacatca gcaccaacca tattgccgcc cacttgggca tcagtccggg
    21781 caacctctat taccacttcc gcaacaaaga cgaaatcatc gtccaactgt tcaaacgtta
    21841 cagcgaagcc ctgctggcat acctgaatga agccgtgttg ccgtctgatg tggaagactc
    21901 catcaattat atggccggta tttatgatgt gatgtgggaa taccgcttcc tattcagcga
    21961 cgtgaacacc ctgcttgcac gcagtgccga attgttgggc gaacacaata ccttcaccca
    22021 agccaaagtc tccccgctct tggtcaacct gctcacccaa ctcaacggtc tgaacatcat
    22081 ccaagccgac caaaccgcca tgaacgatct cgccgtcaat atgtggatgg tcacgaaata
    22141 ctggttcgac ttcgacagct ccctgcgcgg ccgcaccaag ctgaccgaag actccaaagc
    22201 acgcggcatc cgccgcacct taagcctcct tcgtccttat cttttgcccg aacaccgcaa
    22261 gaaatacgac cggaaaatcg gcaacggcaa cccgtaaacc cacagcgtcc gaatcagcct
    22321 tcagacggca cttacccgac ggtatcagcg aatgcgcgca gtccccccgc cccatatctc
    22381 catcacaggc ctaggttacc tcggcctgcc gctggcacaa aagttttacc aacacggcag
    22441 ccgcgttgcc gccgtcaaac gcagcctgac ttcggacgat atcaatctgc ccatacacct
    22501 cgataccatc gacctcaatc aagacagcgc gtttcaaagc gcgaaccttg cccgagatac
    22561 aagcttttgg cggcaccatg ccaacaaacc cgtttggttc tgcctcttgc cgccatcatc
    22621 gctgacacat tacgccgata ccgtcaaaca atgggcagaa cttgcccggg cgtgcaacgt
    22681 gcaacacctg attttcacaa gcagtaccag cgtttacggc gatacagcgc gcgaatgcga
    22741 cgaaatcgcc ctacccgatc cgcaaaccga gtccgcccgc caaatcctcg ccgccgaaca
    22801 acacctgctc gacagcggcg ttccgaacat cgacatcctg cggctgggcg ggctttattg
    22861 cgccgaacgc catcccgtcg gccgccttgt tcaaaagcaa aacatcccgg gcggcaaccg
    22921 ccccatcaac atcgtccacc gtaatatcgc cgtcgaaagc ctgtttcaga cggcatttaa
    22981 ccccggcggc aggcggctga aaaacattat cgaaccgcgc cacccgacac gacgcgaatt
    23041 ctatacggaa gaagccgcca aactcggctt gcccgcgccg gattttgcac ccgacgacag
    23101 cgtgggcaaa atcatccgta ccgtttgcga taacggctta agcctgtaaa ataagcggca
    23161 acagcaacaa acaggcattc tccgccccga tcagaaaata tgtccgccct cctccccatc
    23221 atcaaccgcc tgattctgca aagcccggac agccgctcgg aacttgccgc ctttgcaggc
    23281 aaaacactga ccctgaacat tgccgggctg aaactggcgg gacgcatcac ggaagacggt
    23341 ttgctctcgg cgggaaacgg ctttgcagac accgaaatta ccttccgcaa cagcgcggta
    23401 cagaaaatcc tccaaggagg cgaacccggg gcgggcgaca tcgggctcga aggcgacctc
    23461 atcctcggca tcgcggtact gtccctgctc ggcagcctgc gttcccgcgc atcggacgaa
    23521 ttggcacgga ttttcggcac gcaggcagac atcggcagcc gtgccgccga catcggacac
    23581 ggcatcaaac aaatcggcag gaacatcgcc gaacaaatcg gcggattttc ccgcgaatcc
    23641 gagtccgcaa acatcggcaa cgaagccctt gccgactgcc tcgacgaaat aagcagactg
    23701 cgcgacggcg tggaacgcct caacgaacgc ctcgaccggc tcgaacgcga catttggata
    23761 gactaacctt cagacggcat ccgacatgaa cagccctttt cacaacatcg gcatcgtaac
    23821 ccgccccaac acgcccgaca tccaagacac cgcacacacg ctgattacct ttttgaagca
    23881 gcacggcttt accgtctatc tcgacgaagt cggcataaag gaaggctgca tctataccca
    23941 agacaccgtc ggctgccata tcgtcaacaa gaccgaactg gggcaatact gcgacctggt
    24001 cgccgtttta ggcggagacg gcacctttct ctccgtcgcc cgcgaaatcg ccctgcgcgc
    24061 cgttccgatt atcggcatca accaagggca tttgggcttc ctgacccaaa ttccccgcga
    24121 atatatgacg gacaagctat tgcccgtttt agaagggaaa taccttgccg aagagcgcat
    24181 cctgattgag gccgcactca tccgcgaagg caaaaccgcc gaacgcgcca tcgccctcaa
    24241 cgatgccgtc ctctcccgtg gcggtgccgg acagatgatt gagtttgaag tcttcgtcaa
    24301 tcgggaattc gtctataccc agcgttcgga cgggctgatt gtctccaccc ccaccggatc
    24361 gaccgcctat tcgcttgccg ccggcggccc catcatgcag gcaggattac acgccttcac
    24421 gctcgtcccc atctgcccac aatccatgac caaccgcccc atcgccattc cagacacgtc
    24481 cgaaatcgaa atcctcgtta cccaaggcgg cgacgcgcgc gtccatttcg acggtcaaac
    24541 ccatatcgac gtgcaaaacc tcgaccgcat caccatccgc cgctaccgaa atcccttacg
    24601 cattttgcac ccgaccgact accaatattt caaaaccctg cgccaaaaac tgcactgggg
    24661 cgagcaatta gtctaagccg gcctttaccc gaaagtatcc atgaatccca aaatttcctc
    24721 cgaccataca gaagatgccg tgcgcctctc caaacgcatg gcgcaactgg ggctttgttc
    24781 acgccgcgaa gccgacggct atatcgaaca gggttgggta acggtcaacg gcaaaaccgc
    24841 cgtactcggt cagaaagttt caccggcaga ccgtatcgaa ctgaacaaga aagcccacga
    24901 acagcaggcg gcacgcatta ccatcctgtt gaacaaaccc gtcggctatg tcagcgcaca
    24961 agcggaaaaa ggctataaat ccgccgccga actgattacc cctgaaaatc actgggaagg
    25021 cgataccggc cgcatccgtt tcgatccgaa acacaaaatc ggcctcgccc ccgccggcag
    25081 gctggacatc gactcggtcg gattgctggt attgactcag gacggccgta tcgccaagca
    25141 gcttatcggc gaaaacagcg gcagtgaaaa agaatatttg gtgcgcgtgc gcggcaaatt
    25201 ggacgaaaaa ggacttgcct tactgaatca cggattgagt ttggacggcg agaaactgcg
    25261 tcccgcccaa gtagaatggc aaaacgaaga ccaactgcgc ttcgtgttga aacagggtaa
    25321 aaagcggcaa atccgccgta tgtgcgaact ggtcggactg cgcgtcgtcg ggctgaaacg
    25381 catccgcatg ggcaaggtca aactcggcag gctgccgccc ggcaaatggc gttatctcgc
    25441 tcccggcgaa tcgttttaaa ta
//
LOCUS       DAWGRZ010000009        24772 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000009 DAWGRZ010000000
VERSION     DAWGRZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24772
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.009"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            59..1132
                     /locus_tag="WHT44_000269"
     CDS             59..1132
                     /locus_tag="WHT44_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ2532508.1"
                     /translation="MRPFFVGAAVLAILGALVFFINPGAIVLHRQIFLELMLPAAYGG
                     FLTAALLDWTGFSGNLKPVATLMAALLLAASAILPFSPQTASFFVAAYWLVLLLFCAR
                     LIWLDRNTDNFALLMLLAAFTVFQTAYAVSGDLNLLRAQVHLNMAAVMFVSVRVSILL
                     GAEALKECRLKDPVFIPNIVYKNIAITFLLLHAAAELWLPAQTAGFTALAVGFILLAK
                     LRELHHHELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMMGGV
                     MMVWLTAGLWHSGFTKLDYPKLCRIAVPILFAAAVSRAFLMNVNPIFFITVPAILTAA
                     VFVLYLFTFIPIFRANAFTDDPE"
     gene            complement(1179..3368)
                     /locus_tag="WHT44_000270"
     CDS             complement(1179..3368)
                     /locus_tag="WHT44_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ2532509.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDMARILSVQTAFVEEKPLPESWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGGMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRSYHGQASHSEFVLNADQQQASDEIQTAFGSFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGKRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPIILGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGYTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPYKCPDCGNQDLTAVGHGTQRVEETLRTFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNKIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESPSRQDLHRAVSLWAQVLQQNRDGKIRWSVDVDPQEA"
     gene            3506..4288
                     /locus_tag="WHT44_000271"
     CDS             3506..4288
                     /locus_tag="WHT44_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ2532510.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            complement(4701..6635)
                     /locus_tag="WHT44_000272"
     CDS             complement(4701..6635)
                     /locus_tag="WHT44_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ2532511.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIRRLHEAGHTVIMVTHDPDIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAMSVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(6701..7879)
                     /locus_tag="WHT44_000273"
     CDS             complement(6701..7879)
                     /locus_tag="WHT44_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ2532512.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKENATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLSTGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            8105..8494
                     /locus_tag="WHT44_000274"
     CDS             8105..8494
                     /locus_tag="WHT44_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ2532513.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFKTYDKNKLKQNEYFDITLSEVSPDHFTLKATPNPTTND
                     GETCVVTLNDGGTIAASDTNQSCPGFD"
     gene            8777..9823
                     /gene="adhP"
                     /locus_tag="WHT44_000275"
     CDS             8777..9823
                     /gene="adhP"
                     /locus_tag="WHT44_000275"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ2532514.1"
                     /translation="MKMQAVVVNKNVAGDAEVIEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SGVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(9988..13473)
                     /gene="smc"
                     /locus_tag="WHT44_000276"
     CDS             complement(9988..13473)
                     /gene="smc"
                     /locus_tag="WHT44_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ2532515.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSGHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGCLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELGNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQLQRIHQE
                     QQQIRVQLEENELQVEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSQ
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARAVPQGFVPPEPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHYWLDGVLCAPDLSYALAHQNDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELENLAPELSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIVTLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIAQLEQQTLDWQERQQELALAYETEFQNDEQHIKLEELS
                     EAVQTLDEEYIVVQEKLAQIQEQGREQYAKVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITLLEEAIAQIDDKTKARFKETFDAVNSKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCRLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            13475..13726
                     /locus_tag="WHT44_000277"
     CDS             13475..13726
                     /locus_tag="WHT44_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532516.1"
                     /translation="MVGKNKKEAYFNGNPAAPPHILPAKPYRIPHPRCRLKPSDGIFN
                     APEPRFRQRPMLGAAFAVLPRRRARYNPPRHPPDLETCL"
     gene            13723..14445
                     /gene="lpxH"
                     /locus_tag="WHT44_000278"
     CDS             13723..14445
                     /gene="lpxH"
                     /locus_tag="WHT44_000278"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ2532517.1"
                     /translation="MKPAYFISDLHLSEKQPELTALLLRFLRSSAARQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQNFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLATKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFGAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKY"
     gene            14714..16300
                     /locus_tag="WHT44_000279"
     CDS             14714..16300
                     /locus_tag="WHT44_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ2532518.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(16469..16672)
                     /locus_tag="WHT44_000280"
     CDS             complement(16469..16672)
                     /locus_tag="WHT44_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ2532519.1"
                     /translation="MDNLNPQEISVLPENLPLYCSGPGNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            16788..17285
                     /locus_tag="WHT44_000281"
     CDS             16788..17285
                     /locus_tag="WHT44_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ2532520.1"
                     /translation="MTALTLPEDIRQQEPSALLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIPSLRKQFPEFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            17367..18560
                     /locus_tag="WHT44_000282"
     CDS             17367..18560
                     /locus_tag="WHT44_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ2532521.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVGLLKAKG
                     ASQNFDFIIKQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDNNIDYLCES
                     IVKVL"
     gene            complement(18964..19899)
                     /gene="hemC"
                     /locus_tag="WHT44_000283"
     CDS             complement(18964..19899)
                     /gene="hemC"
                     /locus_tag="WHT44_000283"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ2532522.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYTRLEEMPEGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHGVTNACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            20018..20527
                     /gene="ybeY"
                     /locus_tag="WHT44_000284"
     CDS             20018..20527
                     /gene="ybeY"
                     /locus_tag="WHT44_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ2532523.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPEQHFAHLTIHGTLHLMGYDHIEDDEAEIMEAEEIRLMRAA
                     GFPNPYQED"
     gene            20535..21359
                     /locus_tag="WHT44_000285"
     CDS             20535..21359
                     /locus_tag="WHT44_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEZ2532524.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(21488..22867)
                     /gene="nhaC"
                     /locus_tag="WHT44_000286"
     CDS             complement(21488..22867)
                     /gene="nhaC"
                     /locus_tag="WHT44_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ2532525.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSSFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPNVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVILALMRINAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(23158..24381)
                     /locus_tag="WHT44_000287"
     CDS             complement(23158..24381)
                     /locus_tag="WHT44_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ2532526.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         5672 a         7079 c         6369 g         5652 t
ORIGIN      
        1 ggacggcttg atttgagttt tggtattttt gcccgacggg gtgaaaaata cagttgctat
       61 gcggccgttt ttcgtcggcg cggcggtgct tgccatactc ggtgcgctgg tgtttttcat
      121 caaccccggt gccatcgtcc tgcaccgcca aattttcttg gaacttatgc tgccggcggc
      181 atacggcggt tttttgactg cggctttgtt ggactggacg ggtttttcgg gtaacctgaa
      241 acctgtcgcg actttgatgg cggcattatt gctcgccgca tccgctatac tgcccttttc
      301 gccgcaaact gcctcgtttt tcgtcgccgc ctattggctg gtgttgctgc tgttctgcgc
      361 ccggctgatt tggctagacc gaaacaccga caacttcgcc ctgctaatgt tacttgccgc
      421 gttcactgtt tttcagacgg catatgccgt cagcggcgat ttgaacctgt tgcgcgcgca
      481 agtgcatcta aatatggcgg cggtgatgtt cgtatccgtg cgcgtcagta ttcttttggg
      541 cgcggaagcc ctgaaagaat gccgtctgaa agaccctgtt tttattccaa atatcgttta
      601 taaaaacatc gccattactt tcctgctctt gcacgccgcc gccgaacttt ggctgcccgc
      661 gcaaaccgcc ggttttaccg cgctcgccgt cggcttcatc ctgctcgcca agctgcgtga
      721 gcttcaccat cacgaactct tacgtaaaca ctacgtccgc acttattacc tgctccaact
      781 ctttgccgcc gcaggctatt tgtggacagg cgcggcgaaa ttacaaaacc tgcccgcctc
      841 cgcgcccctg cacctgatta ccctcggcgg catgatgggc ggcgtgatga tggtgtggct
      901 gaccgccgga ctgtggcaca gcggctttac caaactcgac taccccaaac tctgccgcat
      961 tgccgtcccc atccttttcg ccgccgccgt ctcgcgcgct ttcttgatga acgtgaaccc
     1021 gatatttttc attaccgttc ctgcgattct gaccgccgcc gtattcgtac tgtatctttt
     1081 cacgtttata ccgatatttc gggcgaatgc gtttacagac gatccggaat aaaaatgccg
     1141 tctgaaacgg ttttcagacg gcatcggatt gccaataatc aagcctcctg cggatcgaca
     1201 tccaccgacc atctgatttt gccgtcgcgg ttttgctgca acacctgcgc ccacaaactc
     1261 acggcacggt gcaaatcctg tcgggacgga gattcgagga agatttgcgc gcgttcgcgt
     1321 tcggcgaggc gcaccatcag catcggggcg gcgccgaact gtgaaacgct ttcgggcaaa
     1381 agcggggcga gggtttcttt ggcggcattg agaaactcca tcgcatcggc aacgcgcggc
     1441 gcgtcggcgc ggacggcggt ctggaaaccg aagggcggca tggcgaacat ttgccgctcg
     1501 ttcaattcgt tttcggcaaa cacggcgtag tcctgcgctt tgacggcggc gaagacggga
     1561 tgttcgggca gttgggtctg tatcaacacc ttgccgggtt tgtcggcgcg ccccgccctg
     1621 ccggacacct gcatcagctc ggcgaacagc ctttccgggg cgcgaaagtc cgcgctgtac
     1681 aggctgccgt cagcgttcaa cacgataacg aggttgagcc gcgcgaaatc atgccctttg
     1741 gcgagcatct gcgtgccgac caaaatgtcg attttgttgt cggcgatgcg gcggtacaaa
     1801 tccgcccagt cgtttttgtg cgcggtgctg tccctgtcaa cacggacgac ggctgccttg
     1861 ggcaggaagg tgcgcagggt ttcttcgacg cgctgcgtgc cgtggccgac ggcggtcagg
     1921 tcttggttgc cgcagtcggg gcatttgtac gggatgggtt cgcggtggtc gcagtggtgg
     1981 cagcgcagtt ggcgggcgcg ttggtgcagc accattttgg cggagcagtt cgggcagccg
     2041 aaggtataac cgcagtcgcc gcaaaacagc gcgggcgcga agccgcgacg gttgaggtac
     2101 accaacgaca tgccacctgc ttcaaagttc tgtttcaaaa gctgcaaggc ttgcggcgag
     2161 aagccgttgt caagtttcag acggcctacg ttgaggatgt ccacttgcgg cagttgcgcg
     2221 gcggtatggg cgcgttcggt cagttgcagc aggcggtacg cgccgctttg cgccttgtgc
     2281 cagctctcca agctgggggt ggcactgccc aatatgatcg ggcagccgcc ctgcttcgcc
     2341 cgccacaccg ccaaatcgcg ggcgtggtag cgcaattcgt tgtcctgttt gaacgagccg
     2401 tcgtgttcct catcgaccac aatcagcccg acatcatcca tcggcgtgaa caccgccagc
     2461 cgcgtgccga tgaccaattt cgcctgcccc aacatcgcgc gcaaataatc ctgcgtgcgc
     2521 ttgcctgccg ccatctgact gtgcaacacg gcggtcggca cgtcggcaaa acggttttcc
     2581 acccgcttca aaagctgcgg cgtgaggttg atttcgggca acagaaacaa cacctgccgc
     2641 ccctgcgcca acactttcgc catcgcatcg aaatacacct cggtcttgcc gctgccggtg
     2701 atgccgtaca gcaaaaacgg ctggaagctg ccgaaggccg tctgaatttc atcggaagcc
     2761 tgttgctggt cggcattcaa cacaaattca gagtgcgaag cctgcccgtg gtacgacctc
     2821 aatacaggtt tcgccgcttc cgttgtttca atccaaccct gctccgccca atcttcaatc
     2881 aatttcgccg cctgcgcgtt tacctgcttc aacgctgcca tcgtcattcc gcccgaaagc
     2941 agtgcgtccc acaaagccgc ttttttgttg aaccgagctg gtggcggcgt ttgcgccctg
     3001 cccgcttcgt tcaaagcata aaacaacggc ggctgcggca tttccaccgc gcgcgtttcc
     3061 ttcaaaccct gcggcagcgc ggcaaacacc gcctgcccag tcggatagtg gtaataacgc
     3121 gacgtaaatg ccaacaaatc acgccagctt tcaggcaacg gcttttcttc cacaaaggcc
     3181 gtctgaacac tcaaaatccg cgccatatcc atatcgggcg caatatccgt ttcccacacc
     3241 atcccgacca cggttttatt gcggaaaggc acaagcaccc gcgttcccgg aggaagcgga
     3301 tcggaatggg aataagtcaa aaggccgtct gaaagcggca cgtttacggc gatgcggtgg
     3361 taaatcatac aaacaagcaa atcatttttt gcgtatttta acagccggaa gctattttca
     3421 gacgataaat tgagcctagg cgcaatgcag gcgcaacaga gatttgatac aattccgctt
     3481 ttccaacacg cgcaatcatc tttatatgaa aaccaagtta atcaaaatct tgaccccctt
     3541 taccgtcctc ccgctgctgg cttgcgggca aacgcccgtt tccaatgcca acgccgaacc
     3601 cgccgtcaaa gccgagtccg ccggcaaatc cgttgccgcc tctttgaaag cgcgtttgga
     3661 aaaaacctat tccgcccaag atttgaaagt gttgagcgtc agcgaaacac cggtcaaagg
     3721 catttacgaa gtcgtcgtca gcggcaggca gattatctac accgatgccg aaggcggcta
     3781 tatgttcgtc ggcgaactca tcaacatcga cacgcgcaaa aacctgaccg aagaacgcgc
     3841 cgccgatttg aacaaaatcg acttcgcctc cctgcctttg gacaaagcca tcaaagaagt
     3901 gcgcggcaac ggcaagctga aagtcgccgt cttctccgac cccgattgtc cgttctgcaa
     3961 acgcttggaa cacgagtttg aaaaaatgac cgacgtgacg gtttacagct ttatgatgcc
     4021 cattgccggc ctgcaccccg atgccgcgcg caaggcgcaa atcttatggt gtcagcccga
     4081 ccgcgccaaa gcgtggacgg attggatgcg taaaggcaaa ttcccggtcg gcggcagcat
     4141 ctgcgacaat cccgtcgcgg aaaccacttc cttgggcgaa caattcggct tcaacggcac
     4201 gccgaccctc gtcttcccca acgggcgcag ccaaagcggc tacagcccga tgccccaact
     4261 ggaggaaatc atccgcaaaa atcaataatc catccgcaaa aaaggcttgg aaaattccaa
     4321 gccttttttg cgggaaatgc cgcctgaaaa ccaatccgcc ctgaaagtta gcaagccaac
     4381 aaacacatcc taacccattg aatgattgaa aataataaat tgaacaatac gaagccccgc
     4441 cgccgttccc acgcgggcgg gaatctggaa tattgagtta ccgtgattta tccgaaacaa
     4501 gcaaagccga acgaaccgaa ttcccgtctt tacgggaatg acgggactcg agcgttgtgt
     4561 gcaatcatcg aacccccgcc gccgtcattc ccacgcaagt gggaatctgg aacgtagaat
     4621 ctaagaaacc gtttttatcc aataagtttc tgctccaaaa tgaccggtcc tgcagcttca
     4681 gacggcatct ttgtccaacc tcaatcctgt gccaatgcgt ctatcggatt gagtttggct
     4741 gctttattgg caggcataaa gccgaacgcg atgccgattc cggtcgaaca ggcgaccgcg
     4801 ccgatgacgg acatggcgga aatgtccatc gggaagtcgg ttacaaaatg attgaacacg
     4861 aggctgacgg cggcggacaa acccacgccg accaaaccgc cgatgacgca gattaacacc
     4921 gcctcaatca aaaactgctg caaaatattg ccgcgccgcg cgccgattgc catccgtatg
     4981 ccgatttctt tggtgcgctc ggtaacggac accagcatga tgttcatcac gccgatgccg
     5041 ccgactacca atgaaatcag ggcgatggag gaaatcagca gcttcatcgt accggtggtg
     5101 ctttcgacta tctgcctgat gctgtcgctg ttgttcatga agaaatcttc cgtgccgtgc
     5161 cgcgctttga gcagatcggt cagccctttt tcggcaacct gggtattggc attgtctttg
     5221 attttgacgg tgatggagtt ggtgtggctc tcgcctgtga tttggtgcat caccgtcgta
     5281 tagggcgacc aaagcatcag cacgtcggaa ttgccgaaag cgttttcgtc ttttttcatc
     5341 acgccgatga cggtcaaggg gcgtttcctg aacaaaatgg ttttacccaa cggatccgag
     5401 tccgcaaaga gtttgtcttt gacattttgg tcgatgacga cgacctgcgc gtcttctttc
     5461 acatcgtttt cgtcaaacag ccgccccgtt tccagcttca gtccgcgcac gtcgaaatat
     5521 tgttcgccca cgccgtaaag cgaggcggtc aggtcggtgt tgcggtaagt cagcgtgccg
     5581 ccgctcgaag tcatgggcgt ggcggaagca acgtagcttt gtttggcgat gatttttgcg
     5641 tcgtctatgg tcagggtttt aatcctgccg ctgcgcctgt cgccgaagcc gcgccccggg
     5701 aagatgctga tggtgttcgt ccctatcgaa ctgatgtctt caaggatttt tttctgcgaa
     5761 ccattgccca atgcgacgac ggaaaccacc gacgcgatac cgatgatgat gccgagcatc
     5821 gtcagaagcg aacgcatttt gtgcgccaat actgcttgca ccgacattct gaaggcttcg
     5881 acaaactggt cgtaataaaa cgaccacgaa gctttttccc gaatcctccc gacattgctt
     5941 gcggggattt cgggattttt cgaggtgtcg gaaatgattt cgccgtcccg gatttcgatg
     6001 acgcggttgg cattggcggc gatgtcgggg tcgtgcgtga ccataatgac ggtatgcccg
     6061 gcttcgtgca gcctgcggat gatttccatc acgtttttgc cgctggcggt atcgagcgcg
     6121 ccggtcggct cgtcggcgaa gatgatttct ccgccgttca tcagggcgcg ggcgatggag
     6181 acgcgctgct gctgtccgcc cgagagttcg ccgggcttgt tgccctcttt gcttgccaaa
     6241 cccaaatcct gcaagagttt gtccgcccgc gcggaacgct ctttgccgcc cgcgcccata
     6301 tagacggctg gcagcgcgac gttgtccctt gcggtcagcg agcttaagag gttgtagcgt
     6361 tggaagatga aaccgaagcg ttcgcggcgc aatgccgcca gctcgtcagg ctgcattttg
     6421 gcagtttcga tgccgtcgat tcggtacgaa ccggaaccgg cggtatccaa acagccgagt
     6481 atgttcatca gcgtggactt gcccgaaccg gactgcccga tgatggcgac aaaatcgccc
     6541 ttctctatcg acaggctgat gtctttcaaa atatggacgc ggttctcgcc gctgccgaaa
     6601 tagcggttga tgtttttaca ttcgatcaag ctcataatat tccgtaaatc aataacaaat
     6661 gccgtctgaa accgtttccg tgttcagacg gcatattcgt ttatcggcgc ggcgggccgc
     6721 ctagggcgcg ttcgccgctt tcctgttgct cggcggcggt tatttcggag atgaccactt
     6781 tgtccccctc tttcaacccg ctttttactt cggtattcat actgtctctc ataccggtcc
     6841 ggatttcgcg ttccgccgcc ttgccgtccg cacccaacac gcgcacaaac gccttgccgc
     6901 cgcgattttt cacggtcagc gacggaataa tcagcacatt tttcacgccg tcgatttcaa
     6961 ccgtattctg cgtcgtcatc cccgtggaga gtttgccgtc cggattcggc acaaacgaac
     7021 gggcataata gtagaccgca ttggaagccg tatccgtact gctgttgtag ccgcccgacg
     7081 acatcgtggt cagcccgggg tcgacgctgt cgagcttcgc cttaatcggc gtatccggtt
     7141 cggacaaaat cgtaaacgaa atatcctgcc ccgccttcac cttggtaata tcgccctcgg
     7201 caatctgcat tttgttcaac atcatatcca gattcgccaa ttggacaatc gtcggcgtag
     7261 actgcgccgc gttcacagtc tgcccctctt ccacgagaat cgccaccacc gtgccgtcca
     7321 tcgttgcggt aatgcgcgtg tagcccaatt ccgactcggc ggtattgatg gaaattttgc
     7381 tctgtctgat taaagccttc agctcggcaa cattggcttt ggcggcggca aacgcatcct
     7441 gcgcgctttc caaatcctct ttggaagtcg cgttttcctt ccataacgcc gcctgacgct
     7501 tatatttctt ctccgcgctg cccaatgcaa tctgtgccga caccagcttc gcctgatacg
     7561 tttccaactt ggatttttcc gtattgagcg tattggtctg cgaggtcgaa ttgatttccg
     7621 caatcaaatc gcccttttta acctgttgcc cgagtttgac ataaagtttc ttaatctgcc
     7681 ccgatgcctg cgcgccgacc gataccaggt tggacggcga aatctcccct gttgcagaaa
     7741 ccgtccggct gatgtcgccg cgcctgaccg tttccgtaat ataagcagcc tgcggctcgg
     7801 gcttcagata agaccatccg ccccaaaccg ctgccgccgc gactgccgca acagccgccc
     7861 atttcatcat ttttgccatg ttttcaattc tctgtatgcg tcaatcccat aaatcggaag
     7921 cggattctac cgcaagtccc tgccccatcc aaggcggcaa actgccgaca ataaaaataa
     7981 ccatttgttt taaatggctt atacaatctg ctttcccgaa aatttcaatc cgtttacata
     8041 ttccgccatt tccgatacgg cgttataatg ccgtcgaccg acaaacaaat ggaaacacat
     8101 tgctatgaaa aacgttcaaa aaggctttac gctgctcgag ctgatgattg ccgtcgccat
     8161 cctcggcatc ctgacgctca tcacctaccc ttcctataaa acctacatcc ggcgcgtccg
     8221 cctgtcggaa gtcaggacga ccctgctgca caacgcgcag accatggaac gttactaccg
     8281 ccaaaaaggg acgtttaaaa cctacgacaa aaacaaactg aaacaaaacg aatatttcga
     8341 tatcacttta agcgaagtca gccccgacca ttttaccctt aaggcaacgc ccaatcccac
     8401 caccaacgac ggcgaaacct gcgtcgttac gctcaacgac ggcggtacca ttgccgcttc
     8461 cgatacaaac caatcctgcc ccggcttcga ctaatcctcc gccgaagccc gcaaacccgt
     8521 aaagatgccg tctggacaca gcttcagacg gtatctttac gggtttgccg tccggttttt
     8581 caaacttgaa acctgccgcc cctcatccgg caaccgcccc gatgccgcgc ctttccaagc
     8641 tgttataata taacatataa tctatatttt tcaacaacat accacttgac ctgtgagatg
     8701 cggaagcgta tgcttaccct tatatcggac aacattccgt ttacaggttt ttttgccgta
     8761 actaaggagc gcaatcatga aaatgcaggc agttgttgtg aataaaaatg tagcgggcga
     8821 tgcggaagta atcgaacgcg aggttcgccc gttggaatac ggcgaggcat tggtcgaagt
     8881 cgaatattgc ggcgtgtgcc acaccgacct gcacgttgcg gcaggcgact acggcgaaaa
     8941 accgggccgc gtgttgggac acgaaggcat cggtttggtt aaagaagttg ccgacggtgt
     9001 gaaaaatctg aaagtcggcg accgcgtcag catcgcttgg ctgttccaaa gctgcggctc
     9061 ttgcgaatac tgcaataccg gccgcgaaac cctgtgccgt tccgtattga acgcgggcta
     9121 caccgccgac ggcggtatgg cgacccactg tatcgtgagt gccgattacg cggtcaaagt
     9181 ccctgaaggt ttggatcctg cgcaagcttc cagcattact tgtgccggtg taaccactta
     9241 taaagccatt aaagtttccg gcgttcgtcc gggacagtgg attgccatct acggcgcggg
     9301 cggtttgggc aacttggggg tccaatacgc gaaaaaagta ttcggcgcgc acgttgtcgc
     9361 catcgacatc aacgacgaca aactggcgtt tgccaaagaa accggcgcgg atttggttgt
     9421 caacgccgcc aaagaagacg ctgccaaagt gattcaggaa aaaaccggcg gcgcacacgc
     9481 tgcggtcgta accgccgtat ctgctgccgc attcaactct gccgtgaatt gcgtccgcgc
     9541 gggcggacgt gtggttgcca tcgggctgcc gccggaatcg atggatttgt ccatcccgcg
     9601 tttggttttg gacggcatcg aagtggtcgg ctctttggtc ggcacgcgca aagatttgga
     9661 agaagccttc caattcggcg cggaaggttt ggttgtgccg aaagtccaac tgcgtgcttt
     9721 ggatgaagca cccgccattt tccaagaaat gcgcgaaggc aaaatcaccg gccgtatggt
     9781 gatcgatatg aaaaaagaat gcggctgcgg ccatcaccac tgatttgacg tggcagtaca
     9841 catcgaaatg ccgtctgaac gctgttcaga cggcattttt tatggatttg gatttgattt
     9901 taatctgttc tgtttgaaat accgtctgaa aacccatatc gcaacacttc attaaaaacg
     9961 gcaagattca gccgttctgc aacccgttca aacggcttcc gccatttcca acgcctgttt
    10021 gatgtccacg gcgacgacgc gcgagacacc tttttcctgc atggttacgc cgaccagctg
    10081 ctccgccatt tccatcgtca ggcggttgtg ggagatgtag aggaactggg tttgcgccga
    10141 catttctttg accagcctgc agaaacgcga ggtgttggcg tcgtccagcg gggcatcgac
    10201 ttcgtccaaa aggcagaacg gagcggggtt gaggctgaac agagcgaaca cgaggctcat
    10261 ggcggtgagg gctttttcgc cgccggagag gaggtggatg gtgctgtttt tcttgccggg
    10321 cggacgcgcc ataatggaca caccggcggt cagtaggtcg tcgcctatca ttttgagagt
    10381 ggcttcgccg ccgccgaaca gggtcgggaa gaaggtttgg actttgctgt tgacggcatc
    10441 gaaggtttct ttgaaacgcg ctttggtttt gtcgtcgatt tgggcgatgg cttcttccaa
    10501 aagggtgatg gctgcctgca cgtcttcgct ttggctgcgg tagtagccgt cgcgttcgcg
    10561 cgcttcttcg agttcttgca gggcggcgag gttgaccgcg ccgagtgctt cgatttgttg
    10621 cgaaaggctg ccgatgctgc tgttcaatac tttcggcgat tctttcgcca acgcttcgag
    10681 cgcgtccaaa tcggcggcgc gttcggtcag gttttgatgg tagcgtttgg cgttgatcag
    10741 ggcttcctgc tgctgcaaca aggcggtttg ggtggcggcc tgaagctgcg gcagcttggt
    10801 ttgcagggtt tgcactttag cgtattgctc cctgccctgt tcctgaatct gcgcgagttt
    10861 ctcttgcaca acaatatatt cttcgtccaa ggtctgtacg gcttcgctta attcttcaag
    10921 cttgatgtgc tgctcgtcgt tttggaactc ggtttcatag gcgagggcaa gctcttgctg
    10981 gcgttcctgc cagtcgaggg tttgctgttc aagctgggcg atttgctgcc ggtagttttg
    11041 tttttgctgg ttgagtttgt ggacggcgac ttcggcaagc ccatattggc ggttggcttc
    11101 caacagggca agctgcgcct gtttcagacg gccttgctgc tcttggcggc tgtgcgcggt
    11161 ggtttgctgc tggtgttcga gttcggcggc ggcttcctgc aaggtaacga tgtcgtctga
    11221 aagcccgtcg gacgtgtgtt gcaacacggt ctgttcttcc gccaactgcg ccagttcgcg
    11281 ctcgatgtgt tcgcggcgga tttgcccttg gttggtacgc gctaagagtt cggcggcgcg
    11341 ttgctgcgcc tgactgtatt ggcgcgtgtg ctgctgttgc tgctgcatca ggtttttatg
    11401 ttgcacttca gacgagcgca cggcagcttc cgcctgtttg aacgcggctt cggcggcgga
    11461 aagttcgggg gcgaggtttt ccagttcgga cgcgatgccg tcgaggcgcg ctttttgggc
    11521 aatcaggctt tcctgcgcgg gtttggcata gagcaggacg ctgactttat cgacctgatg
    11581 accttcgggc gtgagccaga tttggtgtgc gcccaaatcg ttttgatgcg cgagggcata
    11641 gctcaaatcg ggcgcgcaca atacgccgtc gagccagtag tgcaatgccg tctgaaacgg
    11701 cggctgcgct tggatttggt tcagcaatgc ctgtacgggc agggattttt tgatgccgcc
    11761 ggagagaccg tctgaaagcc acgccgcctg cccctgcggc aaaggttcgg gcggtacgaa
    11821 accttgcggc acggcgcggg cgtgcaggcg ttctgcaagg atgacggaca aggcgtgctg
    11881 ccactcggca ggcgcggtga tgtgttgcca cagctgcggc gcggcggcgt ggtcggttgc
    11941 ctgccagaag tcggcggctt cctgctgttg cgacaggatt tgcgacaacg cctgctgctg
    12001 cgcctgcaag gtgatgtgtt gctgcttcag gctttggaag cggtttgatg ccgtctgaaa
    12061 cgcctcgcgg gcggcgtgta gagcttcttc ggcggcgatg atttgttctt cgtaatgctc
    12121 ttgctggctt tgcaacaagg cggcggcttc ctgcgcggcg gcggtttcgg cttcgtcggg
    12181 caggttcagg gcttggtttt cctgtttcag acgacctttg cgctcttcgt gcttggcaac
    12241 ggtttgttcg gcatgggcaa gctgctgctg cttcaacgcc agttcgcggc ggatgcggtt
    12301 tgcctcgtcc tgctgggttt ggaaggcggc attgagcgtg gcttgggctt cttccaattc
    12361 gggcagacgc tcttcgtgtt cggcaacctg catcgcccat tccgccagct cggtttgttt
    12421 ttcttcgacc tgcaactcgt tttcttcaag ctgcacgcgg atttgctgct gctcttgatg
    12481 aatgcgttgt aactgcgcct gcgccgcctg cttgtcgcgt tcgatgcgtt gatgcaggtt
    12541 ttgctgatgg cggatttgtt cttccaaacg ggcaatctgc tcgcgcaaca cgccgcgttt
    12601 gttgcccagt tcgtgtaccg cctgctgctg cgactgttcg gcggtctgca aggcgtgtac
    12661 ttcgtcgttt aacgcctgaa cctgcgcggc ggtttcgtcc tgctgcgcct gcaaagactg
    12721 atgctgcgcg gtcgctttgt cggcggcggc aagcgattgc tgccattggg cgtaatcgag
    12781 caaatcctgt tgttgattca actgcgcggt cagggattta tagcgttcgg cggtttccgc
    12841 ctgtttctcc agcttttcca cctgacgcgc caattcgttc tgcaaatcgc ccaaacgctg
    12901 caaatgctcg cgcgtgtctt tcagacagcc ttccgtctcc ttgcggcgtt ccttatattt
    12961 ggacacaccc gccgcctcct cgatataagc gcgcaactcc tccggccgcg cttcgatgat
    13021 gcgcgaaatc atcccctgct cgataacggc ataaccgcgc gcgcccacgc ccgtacccag
    13081 aaacaaatca gtaatatcgc ggcggcgcac ggtctgattg ttgatgaaat aagtcgattc
    13141 gccctgccgc gtcagctgcc gcttgatgct cacctcggca tactgccccc acgcgccctg
    13201 caaactgtgg ccgctgttgt caaacaccag ctccaccgaa gctcttggcg caggacggcg
    13261 cgttgccgca ccgttaaaaa tcacgtcctg catactctcg ccgcgaagct gcttcgccga
    13321 agcctcaccc aacacccagc gcaccgcgtc aatcacattc gacttgccgc agccgttcgg
    13381 cccgataacc gcgacaagct gccccggcac atgaatcgtg gtcgggtcgg taaaagattt
    13441 gaagccggag agtttgatat gggtcaggcg cataatggtc ggaaaaaata aaaaagaagc
    13501 gtattttaac ggaaatcccg ccgcaccacc ccatatcttg ccggcaaaac cttaccgcat
    13561 cccgcaccct cgatgccgtc tgaagccttc agacggcatt tttaacgcgc ccgaaccccg
    13621 attccgccaa cgcccgatgc ttggcgcggc atttgccgta ttgccccgcc gccgggcgcg
    13681 ttataatccg ccacgacatc cgcccgattt ggaaacctgc ctatgaaacc cgcctatttc
    13741 atttccgacc tgcatttgag cgaaaagcag cccgaactga ccgcgctgtt gctgcgtttt
    13801 ttacgttctt ccgccgccag gcaggcgcgg gcggtttaca ttttgggcga tttgtttgat
    13861 ttttgggtgg gcgatgacga agtttccgag ttgaatactt cggttgcgcg tgaaatcagg
    13921 aaattgtccg acaaaggcgt tgccgtgttc ttcgtcaggg gcaaccgcga cttcctgatc
    13981 ggtcagaatt tttgccggca ggcgggcatg acgctgctgc cggattactc ggttttggac
    14041 ttgttcggct gcaaaaccct catctgccac ggcgacactc tgtgtacaga tgacagggca
    14101 taccaacgtt tccgcaaaat cgtgcatcgg aagcggctgc aaaaactgtt cctaatgctg
    14161 cccctgaagt ggcgcacgcg ccttgccacc aaaatcaggc gtgtcagcaa aatggaaaaa
    14221 caggtcaagc ccgccgatat tatggatgtc aatgccgcct ttaccgcgcg gcaggtacgc
    14281 gccttcggtg cggaaaggct gatacacgga cacacccacc gcgagcatat ccatcacgaa
    14341 aacggcttta cccgcatcgt tttgggcgac tggcataacg actatgcttc aatcctccgc
    14401 gtggacgggg acggcgcggt attcgtgccg ctggaaaaat actgaaaatg ccgtctgaag
    14461 cctttcgggc ggcttttttt acacgcccct ttcccacact ccctccgccg catcatcact
    14521 atccttaacc cgcattaata gccattatcg ataaaatgct tgaccgatta cataagatta
    14581 cgtaaagtgt gcaaacgcat aacaattggt cttaccaatt tatcttttaa ccaaaatatc
    14641 gggtgtacgg gatttgaacg tctttccaaa cctcgacgcg ccttatgatg caaatgccta
    14701 aaaaggattc gttatggcac ttttcctcag catattcccc atcgtcctgc tgatttggct
    14761 gatggtgaaa aaaaacagta tgccctccta cgtcgcgctg ccgattaccg cagtgctgat
    14821 ttacgccatc aaacttttct acttcgacga tgcgggcatg ctgctcaacg ccaccgccgc
    14881 ttccggcctc gtcaaaacgc tcacgccgat taccgtgatt ttcggcgcga ttatgttcaa
    14941 ccgtatgatg gaaaccacgg gctgcatcga tgtcatccgc aaatggctgg cgaccatcag
    15001 cccgaacccc gtagcgcaac tgatgattat cggctgggct tttgccttta tgattgaagg
    15061 cgcatccggc ttcggtacgc ctgccgcgat tgccgcgccg attctgatga gcttgggctt
    15121 caacccgttg aaagtggcga ttttcacttt ggtgatgaac tccgtccccg tctctttcgg
    15181 cgcggtcggt acgccgacct ggttcggttt cgcaccgctg aacctgagtg ccgaagacat
    15241 cctcgccatc ggcaggcaga ccggcgtaat gcacttcttc gcaggtttcg tcatccccgt
    15301 catcggcttg ggcttcatcg taccttggtc tgaaatccgc aaaaacttgg gcttcgtcgc
    15361 cattgccgtc ttctcctgca ccattcctta tgtcgcattg gcgatggtca acgaagaatt
    15421 cccgtcgctc gtcgccggcg cgatcggcct gatggtgtcc gtattcgccg ccaaccaagg
    15481 ctggggcttg agcaaagacc acgccaaaga cccgaatgcc gaaaaagtgc cgttcgccca
    15541 agtcgccaaa gcactcgccc ctttgggtat gctgatcggc atgctggtgg ttacgcgcat
    15601 caaacagctc ggcatcaaag gcattttgac cagcaaagaa gaatggttca gcttccaact
    15661 gccgtttgat ttgtccaaaa tcaccgtcag cgactccctg acgattacct tcggcaatat
    15721 tttcggacaa gatgtcagcg cgtcttacca aacgctgtac gtcccggctt ggattccgtt
    15781 tgtgctgacc gtttggattt gcatcctgct gtataaaacc aaattcaaag atgcctggac
    15841 gatttattcc gtaaccttca atcaaaccaa aaaaccgctg cttgccctga tgggcgcgct
    15901 gattatggtt cagctgatgc tggtcggcgg cgacaattcg atggtgaaaa tcatcggtaa
    15961 ggaatttgcc gcaatggcgg gcgaacactg ggtttatttc tcgccgtatc tgggcgcgat
    16021 cggtgcgttc ttctccggtt ccaacaccgt gtccaacctg accttcggcc cgattcagca
    16081 gcaaatcgcc ttggataccg gcctgtccgt taccctgatt ctggcgttgc agtccgtcgg
    16141 cggcgcgatg ggcaatatgg tgtgcctcaa caacatcatc gccgtatgta ccgtattgga
    16201 tgtgaaaaat tccgaaggtg cgattatcaa gaaaaccgtt atcccgatgg cgatttacgg
    16261 cgtgattgcc gtcgtcgcgg caatgatttt cttcctctag gcgaaacgcc accatccata
    16321 gcggcagcac gcggggaacg gaaaccgccg ttcaccgtct gggcggcgcg tcgggcagaa
    16381 tcccggtgcc ggaacaagcc cgccccgcca aacaaatgcc gtctgaaacc gaaaaaggct
    16441 tcagacggca tttttccgcc gtttgcgttc aggcgtaatg atgatgcggc atcttgccgt
    16501 caaggcggta gcgcgtgccg caatacgggc aggcaacgct gcccgattct ccttcgcaca
    16561 aaggcaaaaa cactctcgga tgcccgttcc actgctcgtt gccgggtccc gagcaataca
    16621 gcggcagatt ttccggcaac acggaaattt cctgcggatt cagattgtcc atttgatttt
    16681 cctttgcgtg gttggcgtgc gcctatttta cgccatcggc aggctaaagg atattttcgg
    16741 cgcaaagccg caatccgcta taatccccac ttttcagacg gcataccatg actgcgctta
    16801 cccttccgga agacatccgc caacaagagc catccgccct gctctatacc ctcgtttccg
    16861 cctacctcga acacaccgcc caaaccggcg acgaatccct ctcctgcctg tccgacgacc
    16921 agcacacgct gaccgcattc tgctacctcg acagccaagt cgaagaaggc ggcttcgtgc
    16981 aactcatcgc atccggttac ggcgaatata ttttccgcaa cccgcttgcc gacagcctgc
    17041 gccgctggaa aatcaaagcc gtgccgaaag tcttggacaa agccaaagcc ctctacgaac
    17101 aacacggcaa aaccatcgaa acgctcgccg acggaggcgc agacatccct tccctgcgca
    17161 aacagttccc cgaatttgaa gaatgggacg gcgcatacta cgaagccgcc gaacaagacc
    17221 tgcccctgct tgcagaacac atacagtcaa actgggaaac cttcgcccat atcgggcagg
    17281 cgtgattgcg tctgtttcca gcccgtgtaa aacagcgtaa aatcggcaaa cccgaatgac
    17341 cttccgtccc catcaaggag caagctatgt tcttcaagca catcgaagcc gcccccgccg
    17401 atccgattct cggtttgggc gaagcgttca aagccgaaac ccgccccgaa aaagtcaacc
    17461 tcggcatcgg cgtttataaa gacgcatccg gcgcgacacc cctcgtcaaa gccgtcaaag
    17521 aagccgaaaa acgcctgttg gaaagcgaaa ccaccaaaaa ctacctcacc atcgacggcg
    17581 ttgccgacta caacgcgcaa acccaaatcc tgctgttcgg caaagaccac gaaatcatcg
    17641 ccagccgtcg cgccaaaaca gcgcaaagcc tcggcggtac gggcgcattg cgtattgcgg
    17701 ccgagtttgc caaacgccag ttgaacgcgc aaaccatctg gatttccaat ccgacttggc
    17761 ccaaccacaa cgccatcgcc aaagcggtcg gtatccaaga caaaccttat cgttactatg
    17821 atgccgccaa acacggtttg gattgggacg gcatgattga ggacttgagc caagcgcaaa
    17881 aaggcgacat cgtcctgctg cacggctgct gccacaatcc taccggcatc gaccctacgc
    17941 ccgaacaatg ggaaactttg gcaaaacttt ctgccgaaaa aggctggttg ccgctgtttg
    18001 actttgccta ccaaggcttc ggcaatggtt tggaagaaga tgcctacggc ttgcgcgtgt
    18061 tcttgaaaca caatacagaa ttgctgattg ccagctctta ttccaaaaac ttcggtatgt
    18121 acaacgagcg cgtcggcgcg ttcactttgg tggccgaaga tgaagaaaca gcagcccgcg
    18181 cccacagcca agtcaaaacc atcatccgta ccttgtattc caacccggct tcacacggtg
    18241 cgaacaccat tgcgctggtg ttgaaaaatg atgatttgaa agcacaatgg attgccgaac
    18301 tcgatgaaat gcgcggccgc atcaaagcca tgcgccaaaa atttgtcggg ttgctcaaag
    18361 ccaaaggtgc aagccaaaac tttgatttca ttatcaaaca aaacggtatg ttctctttca
    18421 gcggcttgac tcccgaacaa gtcgaccgcc tgaaaaacga gtttgccatt tatgccgtcc
    18481 gctccggccg catcaacgtc gccggcatta ccgacaacaa catcgattat ctgtgcgaaa
    18541 gcatcgtgaa agtactgtaa aaagcacagc catatgaaaa tgccgtccga accttaacca
    18601 gttcagacgg catctgtttt ttgttaaaaa acaataaacc ataacaattt caaacaagct
    18661 aaaaacatct ataaaatcaa ccaaatatga tgaactcaaa taaaaaatac gaatatcatc
    18721 attctcaaat tcaaaatttg aacgccacag tattatccga agccacaaaa acctttccgc
    18781 cgtcattccc gcgcaggcgg gaatccggga atccaatgct gcaagaattg attagaaaca
    18841 actgaaaccg agcaaactgg attcccgcct gcgcgggaat gacgaaataa ggcgagtgaa
    18901 actgatgaaa caaattgatt aaaaacaata acttatatta ttttatttaa attttcgata
    18961 aaatcaattt tccgtattca atactgctcc aatcaattcc cgcgcaccgt cgtccgccaa
    19021 tttcttagcg acagcgcgtc cgagcgcgtc ggcatattcg gcaggggctt gcgcgtccgc
    19081 ccgcaacaca accgaaccgt cggggtgtcc gaccaagccg cgcaaggtca gcaagccgtt
    19141 ttcttccgtg caatatgcgg ccaaaggcac ttggcagctt ccgcccaaag cgcgtgcgag
    19201 ggcgcgttcg gcggtaacgc aggcattggt aacaccgtgg ttcaagggtt tcaaaacttc
    19261 atacaaatct tcgcggtgcg cggcaatttc gatacccaat gcgccttgtc cggcggcagg
    19321 caggctgtcg gattccgaca aaatcatgcg gatgcgtccg tccaatttca gacgctgcaa
    19381 accggcggca gccaagataa ttgcgtcgta ttcgccgtta tcgagtttgg acaaacgggt
    19441 ttgcacattg ccgcgcaaag gtttgataag caaatgcgga tagcgcgcac gcaactgggc
    19501 ttcgcggcgc aggctggatg tgccgacaac cgcgccttcg ggcatttctt ccaaacgcgt
    19561 gtattggttg gacacaaacg cgtcaaacgg attggcgcgt tcgccgatgg cggcaagcgc
    19621 gaaaccttca ggcaaatcca tcggcacgtc cttaatcgaa tgcaccgcca aatcggcgcg
    19681 cccgtcatat aaagcctgtt ccaactcttt gacaaacaag cctttaccgc cgacttttga
    19741 caaagttttg tccaaaatct gatcgccgcg cgtggtcatg cccaaaatct cgacttcgca
    19801 atcgggatac agagccttca gacggccttg gatatgcttt gcctgccaca tagcaagcag
    19861 gctttcgcgg ctggcgatga cgagtttttt cgggttcatg ttcaatgcgt tcaaagaatg
    19921 gaaacggcgt gaagtctatc atattttgaa cgtttcttca gacggcaccg tcaggtctat
    19981 gttatattta cacccacaaa acacgaaacc cgccacaatg aaacgcacca aaaaataccc
    20041 tttcttaacg ttgcagcggc aacgtttcca tttgaacttt gaaaacgcct cttccgccgc
    20101 cggcatccca gccgaacgcg atttctaccg ctgggcgtgg tctgccttga aaaatgaata
    20161 ccgccgcgcc gacatcagcc tgattcttct ggacgaagaa gaagcccgag cctacaaccg
    20221 cgactaccgc ggcaaagatt acgccaccaa tgtattgagt ttcgcgctca acgaaggcga
    20281 aatcctgccc tgccaagttt cggaaaaact gtacggcgat ttgattatct gcccgcaagt
    20341 ggttttgaaa gaagccgccg aacaaggcaa aacacccgag cagcattttg cccacctgac
    20401 catacacggt actttacacc tgatgggcta cgaccacatc gaagacgacg aagccgaaat
    20461 aatggaagcc gaagaaatcc gcctgatgcg ggcggcaggc ttccccaacc cctaccaaga
    20521 ggactgacat taaaatggac ggcgcacaac cgaaaacgaa tttttttgaa cgcctgattg
    20581 cccgactcgc ccgcgaaccc gattccgccg aagacgtatt aaacctgctt cggcaggcgc
    20641 acgagcagga agtttttgat gcggatacgc ttttaagatt ggaaaaagtc ctcgattttt
    20701 ccgatttgga agtgcgcgac gcgatgatta cgcgcagccg tatgaacgtt ttaaaagaaa
    20761 acgacagcat cgagcgcatc accgcctacg ttatcgatac cgcccattcg cgcttccccg
    20821 tcatcggcga agacaaagac gaagttttgg gcattttgca cgccaaagac ctgctcaaat
    20881 atatgtttaa ccccgagcag ttccacctca aatccattct ccgccccgcc gtcttcgtcc
    20941 ccgaaggcaa atcgctgacc gcccttttaa aagagttccg cgaacagcgc aaccatatgg
    21001 cgattgtcat cgacgaatac ggcggcacat ccggcttggt cacctttgaa gacatcatcg
    21061 agcaaatcgt cggcgaaatc gaagacgagt ttgacgaaga cgatagcgcc gacaatatcc
    21121 atgccgtttc ttccgaacgc tggcgcatcc atgcagctac cgaaatcgaa gacatcaaca
    21181 ccttcttcgg cacggaatac agcagcgaag aagccgacac cattggcggc ctggtcattc
    21241 aagagttggg acatctgccc gtgcgcggcg aaaaagtcct tatcggcggt ttgcagttca
    21301 ccgtcgcacg cgccgacaac cgccgcctgc atacgctgat ggcgacccgc gtgaagtaag
    21361 caccgccgtt tctgcacagt ttaggatgac ggtacgggcg ttttctgttt caatccgccc
    21421 catccgccaa acataaaaat gccgtctgaa accgttgcaa gtttcagacg gcatttcgct
    21481 tatccgctta ttttttgctc aaagtcagcc ccgtccaacc gaataacagg gtcaaagcca
    21541 aactcaaata gcagaaaaag gcatacggca gatattccca aaccggcacg cccagcgcgt
    21601 ggctgatgaa cacgccgcat acgctccacg gtacgagcgg gttaatcacc gtccccgcat
    21661 cttccagcgt ccgcgacaga ttgcgcgaat gcagaccgag cttatcgtaa acgggtttga
    21721 acgtttcacc cgacaacaaa atactcaaat attgctcgcc gatcaggaaa ttaaccccga
    21781 ccgaagtcat ggcaacgctg aacgtcgcgc gtccggcatt cgtcaagaag gtacggatgg
    21841 cctccaacag ggaaggaatc acaccgagcg caaacaacag tccgcccaaa ctcatcccga
    21901 gaatcacgat ggtttgcgtg aaaaacatac tttccaaacc gccgcgcgaa atcagtttga
    21961 caacatcttt aaacgcttcg ccttcgagtt tgtagccgcc gtaaaaccac gcaccgagct
    22021 gacgcagatc gggcgtgctg tgcagatacg ttacagcaac ggcaaccatg acggtaaaga
    22081 gcatggcgac gacggcgttg atgcgcatca atgccaaaat gaccaacagc gcaaacggaa
    22141 tcagcgaata gccgtgcacc aatcccgtgg cttcaagctg gctgcggaag gattcgacgc
    22201 tgttcaaatc ctgcgcggcg acattcggca aaagccaaag catcagtgcc gcactaatga
    22261 gccacgcggg gatggtggtg tacatcatat ttttgatgtg ctcaaacaag tcgatgccga
    22321 cgatggacgc ggaaataccc gtcgtatccg aaagcgggga cattttgtcg ccaaaaaatg
    22381 cgcccgaaac aatcgcgccc gccgtcatcg ccatatcggc ctgaaacgcc gccgccatcc
    22441 ccataaaggc aacgccgaca gtggcgcagg tggtcaggct gctgccgatg gacacgccga
    22501 tgacggaaca cagcgcgaag gaggaaaaat aaaaataagt cggggaaatc agtccgaaac
    22561 cgtaatacat cagcgtcgga atcgcgccgc tcatcatcag cgcgctgacc atcagcccga
    22621 tgaagaaaaa caggtaaatc gcgcccatac cctgattcaa cgcgcctatc atgccctgct
    22681 gcatatcgtt gtatttcaaa ccgcgcgcca agccgtacaa aatcagcacg acgatggcgg
    22741 caataatgga catatgcggc aaccactcca atgaaatgat ggtatagccc atcgcggcaa
    22801 tcagagcgac gacgacggca agtgcctcac cgcgcggcat atcgagtaag gatttgaaag
    22861 cgaacatgat tctttgcccg atttgatata aagttaacgg attctaacac accataccga
    22921 atccgcccga cgttttcaga cggcatttta gcgttttaca aaagccggac agaaacaaaa
    22981 cggcaatttt ctcaatttaa attaattttt ttgaaatata attttttatt tttttgtttg
    23041 ttgacatata agacttttat ggattgaagt attaaaacct cggcacacac gccgcaaaca
    23101 ccggtacgca aaaatgccgt ctgaaagatg ttcagacgac atttctcaag aaaaagatta
    23161 tttgtccgcc ctatatccgt acaccgcaaa aaacacgata tacagataac agatgacgga
    23221 aacgacaaac gaagacatca gggtgtaagt atctgccacc cagccctgca cgacaggaac
    23281 gaccgcaccg ccgacaatcg cggtacacag tacaccggaa gccgcgttgg taaattttcc
    23341 caatcctttg gttgccaaag agaaaatcgt cggaaacata atcgagttga aaaaaccgat
    23401 ggcaagcagc gaccacatcg ccacatccgc attgccgcta cccgtcgcca tcgcgacggc
    23461 aagcagtacg accgcagccg atgcgttaaa cgccaaataa cggttgggcg cgaatttcgc
    23521 catcaccgcc gaaccgagga aacgtccgac catcgcgccg ccccaataga acgacaggta
    23581 atgcgcggca gaagcatgat ccagcccttt cagataaccc aatacgttga ccatcaacga
    23641 accgatagac acctccgcgc cgacatagca gaaaatgcct gccgtaccga acacgagatg
    23701 tttgtattgc catacgctgg ttttgccgtc gtggttgtgc gcgctttcct cggcggcaat
    23761 tttgcgcgcg tcgggcagcc ggatcatttt cacgaaaacg gcaaggataa tcagcagccc
    23821 cgccagtccc aaatagggaa tctgtacgga agaaatctgt tccgccttgc tgacggtttg
    23881 ggttgcgtcc gccagaatca ggaacgcgcc gatttgcggc gcaatggtcg tacccaaagc
    23941 gttaaacgcc tgaaccagcg tcagtgttgc cgattccttg ccgggtttcg ccagcagggt
    24001 aacataagga ttaccggcga cctgaagcag cgttacgccg gaagccaaaa taaacaacgc
    24061 gcccaaaaat accgcgtaag aatggctgcc cgcagcagga taaaacagca ggcatccgac
    24121 cgccgtcagc agaaagccgc cgataacgcc gtttttgtag ccgactttgc cgacaaaagc
    24181 ccccatcggg atggacatca ccgcataggc ggtaaagaaa cagaattgga tcagcatcgc
    24241 ctgaacgtaa gacaggtcga aaatttcttt caaatgaggg ataaggatgt cgttcatgca
    24301 ggtaataaaa cccatcatga agaacagcgt ggtcaagacg accaatgggg acgtatgatt
    24361 gttttgtgat tgcgcagaca tgatttgcct ttcgtggtgt cggaatatta cccgtttcaa
    24421 gaaaatagaa attgacattt cttaacggca atgcaaatca tactgatatt tgaacatttt
    24481 gcaaacttta tcggatgcag gcaatgcgcc gatctgaaat tcaagcagca gaaattaata
    24541 ttttacagtt gcttgcatag attttaatag attttaaaaa attccggcgg acttgcaaaa
    24601 accgttccca ataaaatgaa aatccgccgc cgtatccgga aaattcggaa atgccgcatc
    24661 gtggcatcaa aaccggagcg gaaagaatgt cgtatgcccg acaaaaaagg ggcatacggc
    24721 tgtgccgtat tttttgcacc gtcctccgcc cttcggcggg ggcgtgaaaa ta
//
LOCUS       DAWGRZ010000010        24402 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000010 DAWGRZ010000000
VERSION     DAWGRZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.010"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(287..2128)
                     /locus_tag="WHT44_000288"
     CDS             complement(287..2128)
                     /locus_tag="WHT44_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ2532527.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDIFGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPAFMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            2219..2689
                     /locus_tag="WHT44_000289"
     CDS             2219..2689
                     /locus_tag="WHT44_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ2532528.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            complement(2762..3574)
                     /gene="hpnC"
                     /locus_tag="WHT44_000290"
     CDS             complement(2762..3574)
                     /gene="hpnC"
                     /locus_tag="WHT44_000290"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ2532529.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLQRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYGNFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LNGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKKALWK"
     gene            complement(3661..4143)
                     /locus_tag="WHT44_000291"
     CDS             complement(3661..4143)
                     /locus_tag="WHT44_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ2532530.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            4616..4912
                     /locus_tag="WHT44_000292"
     CDS             4616..4912
                     /locus_tag="WHT44_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532531.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPAAEAPAAEAPATEAPAA
                     EAPAAEAPAAEAPAAEAAATEAPAAEAAATEAPAAEAAATEAPAAEAPAAEAAK"
     gene            5022..6389
                     /locus_tag="WHT44_000293"
     CDS             5022..6389
                     /locus_tag="WHT44_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ2532532.1"
                     /translation="MLDLHTEFSRLLPADEIAEPSPTLLKDQRNRFTSAPDIILQPLS
                     VKSVQTIMRFCHQHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFANPLDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINSTVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(6570..8552)
                     /gene="autB"
                     /locus_tag="WHT44_000294"
     CDS             complement(6570..8552)
                     /gene="autB"
                     /locus_tag="WHT44_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phase-variable autotransporter AutB"
                     /protein_id="HEZ2532533.1"
                     /translation="MVSLFFSILYTSPLLAVDYVYDKTKLTNDEITRLKKLRDKTSEY
                     WKKETYLITEDNPKVPPFPALYPRTYQFENINNSKKISFYDQEYTEGYLVGFARGLGV
                     AKRNGDTEEQIRKYFKECFNSNTKIRDYSTCQAEKFGSHPLIVKSHIFSLGPKIKNSH
                     INSEILSVGNYTEWANQVIHHIENYVSFAAHLYSGLDPFHYIEVTDNSHVIGQTISLD
                     EFRLENSLWEPRWDSNVGKLKTTNADIRFNTKSESLLVKEDYAGGARFRFAYDPKEAK
                     NTALIFEKNVTGTSDIIFENPIDDLKSLDGHQIIKVNGTADKHAFRLSGKHQKGIYTL
                     SLQQRPEGFFTKVQERDDMAIYAQQAQAANTLFALRLNNKNSDIFDRTLPRKGLWLRV
                     IDGHSNQWVQGKTAPVEGYRKGVQLGGEVFTWQNESNQLSIGLMGGQAEQRSTFHNPD
                     TDNLTTGNVKGFGAGVYATWHQLQDKQTGAYADSWMQYQRFRHRINTEDGTERFTSKG
                     ITASIEAGYNALLAEHFTKKGNSLRVYLQPQAQLTYLGVNGKFSDSENAHVNLLGSRQ
                     LQTRVGVQAKAQFSLYKNIAIEPFAAVNALYHNKPFGVEMDGKRRVINNKTAIESQLG
                     VAVKIKSHLTLQATFNRQTGKHHQAKQGALNLQWTF"
     gene            complement(8983..9429)
                     /gene="smpB"
                     /locus_tag="WHT44_000295"
     CDS             complement(8983..9429)
                     /gene="smpB"
                     /locus_tag="WHT44_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ2532534.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(9484..10494)
                     /gene="waaF"
                     /locus_tag="WHT44_000296"
     CDS             complement(9484..10494)
                     /gene="waaF"
                     /locus_tag="WHT44_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ2532535.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENSFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLDIGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINRLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(10625..10700)
                     /locus_tag="WHT44_000297"
     tRNA            complement(10625..10700)
                     /locus_tag="WHT44_000297"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10665..10667),aa:Asn,seq:gtt)
     gene            complement(10892..11644)
                     /locus_tag="WHT44_000298"
     CDS             complement(10892..11644)
                     /locus_tag="WHT44_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ2532536.1"
                     /translation="MITLPSLNNLPSKWDEIRHWLESRVFECGVMPHPNLTECEAKQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGSRGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALEHGEVQTALF"
     gene            11945..12760
                     /locus_tag="WHT44_000299"
     CDS             11945..12760
                     /locus_tag="WHT44_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylethanolamine-binding family protein"
                     /protein_id="HEZ2532537.1"
                     /translation="MTAYNESTYSSSKGIIYANPHPLRRTAGFFNRCLCRGRIHAAIR
                     QPVRRRRLHAKPAFERALRLLLFRRQCFARAVVEKSARRDKSFVLTVYDKDAPTGLGW
                     MHRVVADIPADVHRRNATSLQLSRCANIADRTGLDALGGRRHSRRCPPPQRGLAAIKP
                     LRQHRRRPVRSHIGGNQFADLPHQVDAFVHGKTDAVMLQPRQHAAKRGLRSIVRHFFI
                     RQYRSLIIKRRAHTPGIRQLYRIAHCLRRPVLCGEPFYGFLFESNPLRLIEAV"
     gene            12947..13057
                     /locus_tag="WHT44_000300"
                     /pseudo
     CDS             12947..13057
                     /locus_tag="WHT44_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(13431..14576)
                     /gene="dapE"
                     /locus_tag="WHT44_000301"
     CDS             complement(13431..14576)
                     /gene="dapE"
                     /locus_tag="WHT44_000301"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ2532538.1"
                     /translation="MTETQSLELAKELISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADM
                     KTSIACFVTACERFVAKHPNHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTQAGKLTDVARAAIAETCGIEAELSTTGGTSDGRFIKA
                     IAQELIELGPSNATIHQINENVRLNDIPKLSAVYEGILARLLAGNAV"
     gene            complement(14689..15309)
                     /locus_tag="WHT44_000302"
     CDS             complement(14689..15309)
                     /locus_tag="WHT44_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEZ2532539.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     KLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(15343..15852)
                     /locus_tag="WHT44_000303"
     CDS             complement(15343..15852)
                     /locus_tag="WHT44_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ2532540.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIAQYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(15947..16498)
                     /gene="azu"
                     /locus_tag="WHT44_000304"
     CDS             complement(15947..16498)
                     /gene="azu"
                     /locus_tag="WHT44_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ2532541.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEEASLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            complement(16779..17201)
                     /locus_tag="WHT44_000305"
     CDS             complement(16779..17201)
                     /locus_tag="WHT44_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ2532542.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAAKLEKRHPELAEALANLVRRHGA"
     gene            17332..20082
                     /gene="secA"
                     /locus_tag="WHT44_000306"
     CDS             17332..20082
                     /gene="secA"
                     /locus_tag="WHT44_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ2532543.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSKLLTQAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALEDGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            20097..20249
                     /locus_tag="WHT44_000307"
     CDS             20097..20249
                     /locus_tag="WHT44_000307"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532544.1"
                     /translation="MPSEHPAPVSDGILPEPPHPTAIPKNPDFVPSVPKTDIPSAPHH
                     DSIRLH"
     gene            20227..21999
                     /locus_tag="WHT44_000308"
     CDS             20227..21999
                     /locus_tag="WHT44_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ2532545.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMVVPKVHGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLTQVFQPYPNTALVDTGMVIDNEGRHYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAGRILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMLTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            22186..24114
                     /gene="rpoD"
                     /locus_tag="WHT44_000309"
     CDS             22186..24114
                     /gene="rpoD"
                     /locus_tag="WHT44_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ2532546.1"
                     /translation="MSRNQNHEEYQDDTRPLSIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            24261..>24402
                     /locus_tag="WHT44_000310"
     CDS             24261..>24402
                     /locus_tag="WHT44_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ2532547.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLR"
BASE COUNT         6356 a         6764 c         5659 g         5623 t
ORIGIN      
        1 tgattaacta ttttttaatc atgttattat tttccataaa atacatgaca ttaagatgtt
       61 tttccacaaa agatacacac accggcaaac accggctgtg tttatctttt cttatgccta
      121 ttttttaatc atcgtatttt tatcttttaa tttcaatacg caaactaact tatacacacg
      181 gttttcacat ctttagactg cttccgtgtg tatagtggat attgccgttt tcctttctga
      241 caaaaatgcc gtctgagaac ttcagacggc atttgaaaca tcggaatcag cggttttgtt
      301 cataccactc gataaacttg tctgctttga caaaacccag cagcggctcg ctgcggctgc
      361 cgtcggagcg gacgacaaac acgcccggcg gcccgaacag accgtattct ttcaacaacg
      421 cctgatgttc gggcgtgttg gcggttacgt cgatttggaa aaagcgttcc atatcgactg
      481 cctgatgcac ttccggctga ttgagcgtgt aagccgccat ttctttgcag gaaatgcacc
      541 agtcggcata aaaatccaaa acgacgggtt tgtcgggatg ttctttcaac gccgtatcca
      601 tcgctgcctt cagcgcggca gtatcggcaa acattttgcc gtgttccgaa gatttgcctg
      661 cttcggctgg tggattgagg gtcaggaaat ggtgcagcgc ggtcgttttg ccgtttgcgc
      721 cctgccagcc gaaccacgcg ccgcctatca gcaatatacc gcccaatgcg aatgccacag
      781 ctttcggacg gcgtttctgc ctgcgtccgt tgaccagcag cataaaggca ggaaccagca
      841 tcagcagcgt gtacagcgcg acgacgagat aatagggcaa gtgcggcgtg gcgaggtaaa
      901 cggcgacggc tagcaggatg aagccgaatg cgtatttgac ggcattcatc caatcgcctg
      961 ccttaggcag gatatgcccg ccgaacgtgc cgatggcaat cagcggaacg ccggtgccca
     1021 acgccaaagt gtaaagtgcc aaaccgccta aaaccgcatc gcccgtctga ccgatgtagc
     1081 ccaaagcaaa tgccagcggc ggggcgacgc acggcccgac aatcagcgcg gacaatatgc
     1141 ccataataaa gacggaaacg attttaccgc ctgaaagcct gctgctttga ttctgaaaat
     1201 acgactgcac ggcgttggga agctggatgt tgaacagccc gaacatagac agtgccaaga
     1261 cgaccattaa agccgatgcc gccaatacca cccaagcctg ctgcaaccat acggtcagca
     1321 gtgcgcccgt cagtccggca acaatgccga ccagcgtata agtcagagcc aaaccctgaa
     1381 cataaacgac ggacagcaca aacgcccgcg ccttgcccgc ctttttgtcg ccgaccacaa
     1441 tactggaaac aatcggcaac aggggataca tacaggcggt aaaactcagg cccaaaccag
     1501 cgagaaaaaa cgccaaaaga ttggcgttga gcgtatccca agacagcttg aaacggctgt
     1561 cgccgccctc atcccccttc gggggcggca gcgccccgct gccgttttga gaggaaggct
     1621 gcaaaaagcg gtctttggcg gatgccggtt cgtcggtttg cggatggtaa gtgccgttgc
     1681 cgaaaatatc aaactcggta tccacgggcg gatagcacac gccggcttcg gcacagccct
     1741 gataggtcaa aaccaattta tacggttcgc cgacagcctt tgcataagga aaggcaacct
     1801 gcgcctcgtg atggtaaacc gtctgcctgc cgaaaaactc gtcttccttc tcttcgccct
     1861 tgctgaaaga aggctgtccc aacaaatccg ccggatcggt cttgccgacg attttcgcct
     1921 gatacatata gtatccgtcg gcaatcctga aacggacgtt cacaccgtcg tcggcaacgg
     1981 caagctccgg cacgaatgcc ttttccggcg gcagcagatc gttcgcatcc agcgcgaaag
     2041 ctcgtccgca caacatcaaa aatacggcga acaggcaaat cagttttttc ataatcgaat
     2101 ccgtttcaga caaataattt gtctgcatta taaatggtaa ggttgacggt gggatttaat
     2161 ttatgtaaaa cccgccatta tccgaaccta tttccataaa catcttatcg aacccgccat
     2221 gtacgatgtc aatacccacg atgtccgccg ctttttcgcc cgcgtgtggc agcagcggct
     2281 caatccgctg caactgagcg cactggaaca gaaagccctc cgcattgtcg aagcccatcc
     2341 cgaataccac cgttatctcg aacgcatcga agaccatctg gacaccgact ggctgcccga
     2401 aaacggcgaa agcaacccct tcctgcatat gtcgctgcat ctgtccgtcc aagaacaggc
     2461 gggcatagac cagccgcacg gcatacgcgc aatccacgac accctgtgcg ccaaacgcgg
     2521 ctggctggaa gccgaacacg aaatgatgga ggcactggcg gaaacactgt ggacggcgca
     2581 acgctacggc accggtttgg atgtcaattt ctacatgacc cgactgcgca aactcatcgg
     2641 cttgggtgca gaagatcaag ccagattgaa cccgcatgaa atcgcctgac cataccaacc
     2701 gcctgcaaaa tgccgtctga agcggaacaa cccctttcag acggcattca ttttccccca
     2761 atcatttcca caacgccttt ttcagcataa tcaaccaatc cttcttatcc aaaacggggc
     2821 gttgtgcaaa cacatcgtat cggcacgcgt ccagtttctg caaaatcaac tgcgccccca
     2881 acacaatcat acggagttcc aaaccgatac gcccattcag ttcccttgcc aaaggcgaac
     2941 ccgccttcag catacggaac gcacgccgac actcatacgc catcagccgc tgaaacgccg
     3001 catccgcccg tcctgccgcg atctgttcct cagaaacacc gaatttcaac aaatcgtcct
     3061 gcggaatata aaccctgcct ttttgccaat ccacagccac atcctgccaa aaattcacca
     3121 gttgcaaagc cgtacagatg ccgtcgcttt gcgccacgca caccgcatcc gttttcccgt
     3181 acaaagccag cataatgcgt ccgacagggt tggcggaacg ccgacaataa tcggccagct
     3241 cgccgaaatt tccatacctt gttttaacca catcctgaga aaatgcagaa agcaaatcat
     3301 aaaacggctg caaatccaaa ccgaacggca caaccgcctc ggcatccaat cgtgcaatca
     3361 aaggatgcgc cgaccggccg cccgatgcca acacgtccaa ctcgcgctgc aaaccctcca
     3421 accccgccaa cctggcttca gacggcatac tgccctcgtc cgccatatcg tccgccgtcc
     3481 gtgcaaacgc gtacaccgcg tgaaccggct tcctcaacct gcgcggcaaa atcagcgaac
     3541 cgacgggaaa attctcataa tgcccaaccg acataccttc tccatccatc aaacaaaatg
     3601 ccgtctgaaa cggaacaaac ccttttcaga cggcatcaga tacctccaag ctgccggcaa
     3661 tcagtggtgg tgatgaccgt gcgggccgtg gacatgaccg tgtgcgattt cctcatcgga
     3721 tgcatcgcgc acgctttcaa ctgtagcctt aaagcggatt ttcatgcctg ccaaaggatg
     3781 gttgccgtcc accaccgcct tgccgtcggc aacatcggtt acacgataga cgacaacatc
     3841 gccggtttca ggatcgtcgg cttcaaacat catgccgact tcgacttcaa cagggaacac
     3901 gcccgcatct tcgatacgga ccaactccgg atcctgctcg ccgaacgcat cgtcgggcga
     3961 cagcgccaca tcgaccgtat cgccggcatc cttaccgtgc aacgcctctt ccaccaaagg
     4021 gaaaatgccg tcgtaaccgc cgtgcagata cgcaatcggt tcttcggttt tgtccaaaag
     4081 ctgattgttg gcatcataca tctcataatg cagcgaaacc acggaatttt tcacgatagc
     4141 catatttgtc ctttcaggaa cagcagatta attacaggcg cattctaaca caaccgccgc
     4201 gccggccgat taccgttaac ctgttcataa actgtacagc acatatttca atgtaaatct
     4261 ttgttatttt attgcggtgt aactttttta caacattctt aaaaccattc cgacctgtct
     4321 gccgactttc ccaatccgcc ttaataaatc atacaagata ctgaaattat attaatctct
     4381 ataatattta tccctatcga atttttaaca gcaaaaccgt tttacaggat ttatcaatcc
     4441 gcccgccaga aaacttttca ttcaaacctt tttcccatct gtacgacatt gcaatccctt
     4501 attccatagt gcataattac gcaaattcag cgatgaattt ccaacccggt ttgtagtatg
     4561 gtcgataaag acctatttgt ttcaataatt taaattggtt ctaaaggtta ctaaaatgaa
     4621 aaaatccctg tttgccgctg ctttgttgtc tttggttctg gcagcctgcg gcggtgaaaa
     4681 agccgctgaa gctcccgctg ctgaagcacc tgccgccgaa gctcccgcta ctgaagcacc
     4741 tgccgccgaa gctcccgctg ctgaagcacc tgccgccgaa gctcctgctg ctgaagctgc
     4801 cgctaccgaa gcacctgccg ctgaagctgc cgctaccgaa gcacctgccg ctgaagctgc
     4861 cgctaccgaa gcacctgccg ctgaagctcc tgctgccgaa gctgcaaaat aagcattttc
     4921 cgcttgcaaa aaagcaggat acgttcagta tcctgctttt ttgatttttc agacggcatc
     4981 agattccctt cctcaatctt ctccctaccc ttccgacaaa catgcttgac cttcataccg
     5041 aattttcccg actcctaccg gcagatgaaa ttgccgaacc ttctccgacg cttttaaaag
     5101 accagcgcaa ccgctttacg tctgcaccag acatcatttt gcagccgctc agcgttaaaa
     5161 gcgtgcaaac cattatgcgt ttctgccacc aacaccgtat tccggttacg ccgcaaggcg
     5221 gcaatactgg tttgtgcggc gcggcagtat cggaaaacgg cgtattgctg aacctttcca
     5281 aactcaaccg catccgcagc atcaatttgt cagacaactg cataaccgtc gaagcaggtt
     5341 ccgtactcca aaccgtccaa caggcagccg aagcctcaaa caggctgttc ccactcagtc
     5401 tcgccagcga aggctcgtgc caaatcggcg gcaacatcgc ctgcaatgcc ggaggtttga
     5461 acgtattgcg ttacggcacg atgcgcgacc tggttatcgg tttggaagtc gtcctcccca
     5521 acggcgaact ggtttcccat ctccatcccc tgcataaaaa caccaccggc tacgacctgc
     5581 gccatctgtt tatcggtagc gaaggtacat tgggcattat cactgccgcc acgctcaagc
     5641 tgtttgccaa ccccttagac aaagcaaccg catgggtcgg catacccgac atcgaatccg
     5701 ccgtccgcct gctgaccgaa acccaagcac actttgccga acgcctatgc agttttgagc
     5761 tgatcggccg ttttgccgcc gaattgtctt ccgaattcag caaactcccc ctgccgacac
     5821 attcagaatg gcatatttta cttgagttga ccgactcatt acccgacagc aatcttgatg
     5881 atcggcttgt cgaatttctt tataaaaaag gctttaccga cagcgtgttg gcgcaaagcg
     5941 aacaagaacg tatccatatg tgggcgttgc gcgaaaacat ctccgcatcg caacgcaaac
     6001 tgggcaccag catcaaacac gatattgccg ttcctatcgg gcgcgttgcc gactttgtcc
     6061 gccggtgcgc caaagatttg gaacagaatt tcaaaggcat acaaatcgtc tgcttcggac
     6121 atctgggcga cggcagcctg cactacaata ctttcctgcc cgaaatcctc agcaatgaag
     6181 tctatcgtta cgaaaacgac atcaacagca cagtctatcg caacgtcctt gcctgcaacg
     6241 gcacgattgc cgccgaacac ggcataggta tcatcaaaaa acagtggctg gacaaagtac
     6301 gcacgcctgc cgaaatcgcc ctgatgaaaa gcatcaaaca acaccttgat ccatataaca
     6361 ttatgaatcc gggcaaactg cttccgtaac cggcatttct gatttgcata cacaacaaag
     6421 aaagggacaa tagatccgat tgtcggttta gcgcgagctc gtgagtgcgg ttaaaaattg
     6481 gtggaaatta cacgaaaaat gaccgcactt ttaaaataaa aaaatcggca gtgaatttcc
     6541 ctgccgattt tattttgtta caacttaact taaaacgtcc actgtaaatt caacgcacct
     6601 tgtttagctt gatgatgttt gcctgtttgg cggttgaatg tggcttgtaa ggttaagtga
     6661 gatttgattt tcactgctac acctaattgg ctctcaattg ccgtcttatt gtttatcact
     6721 cgacgctttc cgtccatttc cacaccgaaa ggtttgttgt ggtaaagcgc gttcacagcg
     6781 gcgaaaggtt caatagcgat atttttatag agtgaaaatt gagctttagc ttgaacgcca
     6841 acccgagttt gtaattggcg ggagccaagt aaattcacgt gggcattttc gctatcgctg
     6901 aattttccgt ttacccccaa ataagtcaat tgtgcctgtg gttgtaggta aacacgaagg
     6961 ctgttgccct ttttagtgaa gtgttccgcc aataacgcat tgtaacctgc ttcaattgag
     7021 gcagtaatac cttttgaagt aaaacgttct gtaccatctt cagtgttgat acggtggcgg
     7081 aagcgttgat attgcatcca gctatccgca tacgcacctg tctgtttgtc ctgaagttgg
     7141 tgccaagtgg cgtaaacgcc tgcaccaaag cctttcacat ttcccgttgt aagattgtct
     7201 gtatctgggt tgtggaaagt gctacgttgt tctgcttgtc cgcccattaa gccaatagaa
     7261 agttgattac tttcgttttg ccatgtgaat acttcgccgc cgagttgcac acctttacga
     7321 tagccttcta caggtgctgt tttgccttgc acccattggt tggaatgtcc gtcaatcaca
     7381 cgcaaccaca agcctttgcg tggtaaagtg cggtcgaaaa tatcgctgtt tttgttgttc
     7441 aaacgcaagg cgaataaggt attggcggct tgagcctgtt gtgcataaat cgccatatca
     7501 tcgcgttctt gcactttggt aaaaaagccc tctgggcgtt gttgtaaaga aagcgtataa
     7561 attccctttt ggtgtttgcc agaaagacgg aatgcgtgtt tatctgctgt gccatttact
     7621 ttgataattt gatgcccatc gaggcttttt aaatcgtcta ttggattttc gaagatgatg
     7681 tcggaagtgc cagtaacatt tttctcaaaa attaatgcag tatttttcgc ttctttagga
     7741 tcgtaagcaa aacgaaaacg agctccgcca gcataatctt cttttacgag taaactttca
     7801 cttttagtat taaaacggat gtctgcattc gttgttttta atttcccaac attagaatcc
     7861 caacggggct cccagagaga attttctaag cggaattcat ccaaactaat cgtttgcccg
     7921 ataacgtgcg agttgtctgt aacctcaata tagtggaatg gatctaaacc agaatataga
     7981 tgtgctgcaa aagaaacata attttcaata tgatgaatta cttgattagc ccattctgta
     8041 taattcccga cagataaaat ttcgctgttg atatgactat tttttatttt tggacctaag
     8101 gagaatatat gactttttac tataagagga tgggatccaa atttttcagc ttggcaagta
     8161 ctataatcac gtatcttagt gttagaatta aaacattcct taaaatattt ccgtatttgt
     8221 tcttctgtgt ccccatttct ttttgcaacc cctaaacctc gggcgaagcc aactaggtaa
     8281 ccttcggtat attcttgatc ataaaaagaa atcttttttg agttattgat gttttcgaat
     8341 tggtatgttc tagggtatag tgcgggaaag ggtggaactt ttggattatc ctcggttata
     8401 agataagttt cttttttcca atattcactc gttttatcgc ggagtttttt taagcgggta
     8461 atttcatcat tagtgagctt ggttttgtcg taaacgtaat caacagccaa aagcggagag
     8521 gtataaagaa tagaaaaaaa tagacttaca ataaatgatt ttttaaactt ctgcttgctt
     8581 gcttgcttgc ttgcttgctt cgagtttcat aataaatttt cctttgtcaa gtaaaaataa
     8641 atggggcgtg gattttagca taaaactgaa caaaaaatgt catttatctc acatttttct
     8701 ctatttattt cttgtttatt aaaagtaaac gtttgctttt tgctattttg tcaagccagt
     8761 ttgaaaatgt gtataattgc cctcgttatt tacaaaaatt tcaggaaaaa tgaccgcact
     8821 ttacccttgg ctaatgccaa tttatcatca aattgctcaa acctttgacg aaagcttggg
     8881 gcatcatgcc gtgctgatta aagcggatgc tggtttaggt gtagaacgtt tacacatcag
     8941 gcggcagcct tgcccatacc gtctgaagca ctgtttccac aatcagcgcg tatgcttaat
     9001 caaccgctgt ttctcgcgtt tccaatccgc ctctttcata ctctggcgtt tgtcgtgctg
     9061 tttcttacct tttgccaaac cgatttccat cttgattttt ccgcgtgaaa aatgcaaatc
     9121 cagcggcacg atggtgtagc cggcacgttc ggttttgccg attaatttgt tgatttccga
     9181 ctggttcaac aagagcttgc gcggacgtac ggcatctggt ttaatgtgtg tcgaggctgt
     9241 gggcaaagcc gtaatatggc agccgaccag ataaaacgcg tcttttttcc aatagatata
     9301 actctcttta agctgtacgc gcgcggcgcg gattgctttg acttcccagc cttccaagac
     9361 caaaccggct tcaatccggt cttcaatgaa aaaatcgtga aatgcttttt tattgttcgc
     9421 aatagccata aacatcctat caatatccgc cgtcagacgg cataaacccg aaaacagaac
     9481 ccatcatacc gcctcttcaa ccgcctgcac aatcttctcg ggatacagcc tgttgaggca
     9541 gtcggtatgc cccagcggac attcccgctt aaaacacggc gaacattcca agtgcaggct
     9601 gacgattttc gccctatcgc tcaaaggcgg cgtatgcgtc gggctggaag aaccgtaaac
     9661 cgccaccacc ttcctgccca aagctgccgc caaatgcatc aatccgctgt cgttacacac
     9721 gaccgtgtcc gccaacgaca gcaaatccat tgcctgcgac aaatcggttt tgccgcacaa
     9781 attgacacac ataccgtctg aaaggcggtt gatttcctcg gcaatttcat catctttttg
     9841 cgaaccgaac agccaaacct gccaacccgc cgccagataa tgtttgccca actcggcaaa
     9901 atgccttgtc ggccaacgct ttgccggccc gaattccgca cccggacaaa aagccagaac
     9961 aggctttcca atatccaagc caaaggtttc gacagaaatt tcccgccgcc gttcatcaat
    10021 ggaaaactcg gggaatcccg aatgcccgtc aaaatcttcc tgactcggat gcgcgagagc
    10081 cgtatatcga tccaccatca aaggcagacg ttccttatcc agcctgcgta tatcgttcaa
    10141 cagaaaataa cggctttcac cgacataacc cgtcctttta ccgatacctg tcgccagcgc
    10201 gatgattgcc gatttcaaag aaccgggcaa cacgataacc tgatcgtatc cgcgccgccc
    10261 caaatcccta ccgacccgcc aacggcgttt caactccaac gcaccatgtc cgaacgaatt
    10321 ctcaagaatt tcattcactt ccggcatacg ctcgaacacc gccatcgacc acttcggtgc
    10381 gaacacatca atcgtgcaac cggggtgaag ttccttcaaa cggcggaaca agggctgggt
    10441 catcacgcag tcgcctatcc aactggggga aataatcagg attttgatgg acataacaag
    10501 aaaccgaaat cagacaggca gaattttacc gcgaaaccgt tggaaaacct atcttgccgc
    10561 attccgaacg ccggacgtgc aaatatgaaa aagcccgaac attcaagttc gggcttcaaa
    10621 attctggctc cccgacctgg gctcgaacca gggacctgcg gattaacagt ccgtcgctct
    10681 accgactgag ctatcgggga atggggcgta ttatagcgtc cggaaaaaat gtgtcaatcc
    10741 ttaattttgg aaaaatgggc gacaaaacga caagcatatg aatcagaaag acattaagac
    10801 cgatgcctta aaaggattgc cgttgtatga atttccacag ccgtcatcac accatattta
    10861 agcccgatga gccgttctgc cctccccccg cttaaaacaa tgccgtctga acttcgccgt
    10921 gttccaaagc cagtaaaaac tgtttgcggt tcaacccgcc cgcgtagccg gtcagtttgc
    10981 cgtcgctgcc gatgacgcgg tggcagggaa tcaggataga tactttgttc tgcccgttgg
    11041 cggcggcaac ggcgcggacg gctttggggt tgcccaaacg ctgcgcctgc tccttgtagc
    11101 tgcgcgtttc gccgtaagga atcgccaaga gcgcgtccca tgcctgcttt tgaaactcgg
    11161 tgccaatctg ctccaaaggc gtggcaaagg ttttcagacg acccttgaag tataagtcca
    11221 attcctgccg caaaagttgc gtccgctcat cctcccgaaa cacaaaccgt ccgcgcaagg
    11281 ctttttggac ggcggcaatt tcctgttcca aatgcttctg tccgacaaat tccagcaaac
    11341 acaaacccct gctaccgaac accgccagca tctcgcccaa aggcgtggca atggcggcac
    11401 acaccagctc gttcaaactg tcgggataac gcgcttccaa cagacggatg gcgcggcgga
    11461 tgcggacata ttcttcaggc gcgcagccga tattgtccca aaaatcccgc tcgaactgtt
    11521 tggcttcgca ttccgtcaga ttgggatgcg gcataacgcc gcactcaaaa acccgagatt
    11581 cgagccaatg gcggatttca tcccattttg acggcagatt gtttaaggaa ggcagggtaa
    11641 tcatttgttt gctccgtatc cctatcatag atttgacggc aaaatcccca atttttgcca
    11701 ttcccgcacg ccggagcagg aacgggctat gacgtaaatc ttgagggtta ggttgcggca
    11761 atacctaaat attcgatatt tctaaagcat cagagaaagg aatgtttcaa cacacaggac
    11821 gacacataaa gcgccgcccc atgaaaaatt tcagacgacc tgcaaagggt cgtctgaaac
    11881 cacgattttt gcatttgcgc attctggcac atcatccaac cgtttcggca cattcctgcc
    11941 gccgttgaca gcctataatg aatccactta ttcatcaagc aaaggaatca tctatgcaaa
    12001 ccctcatcct ctccgccgta ctgctggctt tttcaaccgc tgcctttgcc gggggcgcat
    12061 tcacgctgca attcgacaac ccgtccgaag acggcggctt cacgcaaaac cagcttttga
    12121 gcgcgcctta cggcttttgc tgttcaggcg acaatgcttc gcccgcgctg tcgtggaaaa
    12181 atccgcccgc cgggacaaaa gtttcgtcct gaccgtttac gataaagacg cgccgaccgg
    12241 actgggctgg atgcaccggg tggtcgccga cattcccgcc gatgtccacc gccgcaacgc
    12301 gacctcgctg caattaagcc gctgcgccaa catcgccgac cggactgggc tggatgcact
    12361 gggtggtcgc cgacattccc gccgatgtcc gccgccgcaa cgcggcctcg ctgcaattaa
    12421 gccgctgcgc caacatcgcc gacgaccagt ccgcagccat atcggcggta atcagtttgc
    12481 ggatttgccg catcaggttg acgccttcgt acacggcaaa accgatgccg tcatgctgca
    12541 accacgccaa cacgccgcaa agcgcggcct ccgcagcatt gtgcggcact tcttcatccg
    12601 ccagtaccgc agcctcataa tcaaacgccg cgcccatacg cccggaatac ggcagcttta
    12661 ccgcatcgca cactgcctgc gccgtcccgt attgtgcggc gaacctttct acggtttcct
    12721 gttcgaaagc aatccattgc gcctgataga ggccgtctga atcgggaata ttgatgacgt
    12781 caaacgtctg tccgcctgcc aaggcgaccg ccttacccgc cgcagcttct tacttccgcg
    12841 ccgcacgata agcacagccg gttcatatac cgccacgctg cggtacaagg cggtatgatg
    12901 ttgcacgatg ccgcctaaag cacccaatcg ttcgcgcgta tgaaagtata gtggattaaa
    12961 tttaaatcag gacaaggcga cgaagccgca gacagtacaa atcgtacggc aaggcaaggc
    13021 aacgccgtac tggtttaaat ttaatccact atatctcaaa cccacgttag gtctaagcaa
    13081 atggtcggac atccttatcc gacagcccat cttcttttca gacggcattg caaatttaag
    13141 tttgacgtgc gttcaaaata aggcagttaa tgcgaagcga aattccgtcg gcgtacctgc
    13201 aacttggccc ctcccctata ggggagggtc ggagggaggg taaaacgggg cagatacaga
    13261 caatatttcc gttgccgccc cgatgccctc tccctaaccc tctcccacgg gagagggaat
    13321 ggattgccgt tgaaataaat cgctctacat aaaaaatcaa tgtgttatct caaacccaca
    13381 ttaggtctaa tcaaatggtc ggatatccat attcggcaag caagctgctt tcagacggca
    13441 tttccagcca acaagcgcgc caatatcccc tcatacaccg cagacagctt cggaatgtcg
    13501 tttagccgca cgttttcgtt gatttggtgg atggtcgcat tggacgggcc taattcgata
    13561 agttcttgcg caatggcttt gatgaagcgt ccgtccgaag tgccgccggt ggtggacaat
    13621 tcggcctcaa tgccgcaggt ttcggcaatg gctgcgcgtg ccacgtcggt cagtttgccc
    13681 gcttgggtca gaaagggctg ccccgaacac gaccactgca aatcgtattg cacgccgtgt
    13741 ttgtccaaaa tggcgtggac gcgttgtttc agccctgctt cggtggactc ggtggagaag
    13801 cggaaattga atttgacgtt cagctcgccc ggaatgacgt tggtcgcgcc tgtgccgccg
    13861 ttgatattgg aaatttgaaa gctggttggc gggaaatatt cgttgccttc atcccagact
    13921 tcctgcgtca gctctaacaa ggccggggca aaagtatgca cgggattgat tgccaaatgc
    13981 ggataggcaa tatggccttg cttgcctttg acggtcaggt tgcccgacag cgagccgcgc
    14041 cgaccgtttt taatcatatc gcccaatttg tccacggcgg tcggttcgcc gacgatgcag
    14101 tagtcgataa gctcgtcgcg cgctttcaat acatcgacga ctttggtcgt gccgtccaac
    14161 gcgtcgccct cttcgtcgga agtaatcaga agcgcaatgc tgccttggtg gttgggatgt
    14221 ttggcaacga agcgttcgca ggcggtaacg aaacaggcaa tgctggtttt catgtctgcc
    14281 gcgccgcgcc cgtataatct tccgtcgcgc tcggccggtt cgaacggggg cgaatcccat
    14341 ttttcgacag gacctgtcgg tacaacgtcg gtatgccctg caaaacagac gacgggagct
    14401 ttcgtgccgc gtcgcaacca gatgtttttg gtgtcgccga aatggagttc ttcagccgca
    14461 aaaccgattt tgtgcaggcg ttcggcaagg agtttttggc aatccctgtc gtcaggggta
    14521 acggatggtc gggaaatcag ctctttggca agctctaggg attgagtttc ggtcatattt
    14581 gttcactttt gaaattagac cgtctgaaac gttctgaatg tgattttcag acggcattta
    14641 ggttaggttg gcatacgggg tgggtatttt acccatcagt cttctgaatc atttgccgtg
    14701 gcaggcttcg taaagcggca gcaaatcttc caccgtttcc gctatccatt tcgcgacatc
    14761 ctgcctgccc aaatcgtcgc gttcgatgtg tttgccgatg cagaaaaagt cttcgtcgtt
    14821 ttgcaacttt cggtcggact cgttttgttg cgcgacggtg cggtagtcgt cgtattcgct
    14881 ttccgcaccg tgccacatat cgaaagaagc gtatttttcg gtatcaaaat tatccaacca
    14941 gcggttgtaa tcaggcagcg cgatggggga aacatcggct ttatagcagt gccaatccaa
    15001 gctgacgctc agacggcggc ggttgagcag tatcgacaaa atcgctgcgg aatttttata
    15061 ttgttcgtat ttgaagtagg caaagaaatg ggcgcgaacc tgccagccgt tacaccagcg
    15121 ttcgatgtgc ggcggcgcaa acggcgcacc caattcggcg gcaacctgct gaatcagctg
    15181 ctgccatatc tgccagtttt ctttatagtc agccttgatt tgcggaatgc tttcaggctg
    15241 gtatttttta agctgggaaa attggaaaaa cgggatattg aacaaatcgc aacttttcgg
    15301 ggtcagcata atatatcctt gagacgattg tttcagacgg cattatttgc gccggcgcgc
    15361 cgccataatt tcgccgattt cggtcagttt ttcttttggg ataaaggtgt tgcccatatc
    15421 aaacagcggc tcttcaatcg ccaaatgaac atcatatccc gccacaaaac gtttgaacgc
    15481 ttcctcatcg gggacataag cgttgtctgc ttcgagtttg gcaaattcgg cggaaacagc
    15541 cgcccagttg tcgtgcagcc cgatatgttg gcgcaaaagc tcgtccacgc tttcttgggc
    15601 ttgcggcgca tattgcagca gcagcgggaa gaagttttct tcttcgtctt catggtgcag
    15661 cggcgcggca acgttgaaat actgggcgat ttggcggatg gtttgcaaaa caatctgatt
    15721 gcagccgttt tcggcgatat agtccgacag catggcgact tgtccgcaaa aacggcgcac
    15781 tttgccgtgg caggcataca gcatttcaat cggttcggca aaggtaacgc ttttggtttc
    15841 aaacggattc atgttttcgt tctcaacggg cacttttcaa gcagtcattt tataataaaa
    15901 cagcctgcac aaagcaggct gtccgtcttt tgagacttta agcggattaa tcgaccaaag
    15961 tcactttgcc gttcatcaaa gcaccgtgac ctgggaaggt acaagcgaat ttatattcgc
    16021 cgtcggccaa tttagcagga tccagagtca gggaagcttc ttcgccgccg ccgatcagtt
    16081 tggtatgggc aacaacgcgt gcatcatcag gtttgacata gtcggtatcg gcagcaccta
    16141 cgccgtcttt aaatacgccg tccatgtctt cagctttggc aatcacgaga ttgtgaccca
    16201 tgctggcttt gggttgcgta ccggtatgtt tcagagtgat ggtgaactct ttacatgctt
    16261 tgctgacttg gatgtctttg gtgttgaact gcatattgtc gttggattcg acagttgccg
    16321 cacagttgcc ggcagcaggg gcttcggcag catctgcagg agcagcttcg gcggcaggcg
    16381 cttcggaagc gggtgcttca gcagcaggag ttgcctcggc agcaggcgcg gcaggttctt
    16441 gagagcaggc agccaaaccg ataacggcgg cagaaatcag agccagatac gctttcataa
    16501 caaatctcca atcgataaaa taatattcgg ttttacagaa atcaaagtgc aaccgccatt
    16561 aacaaaacct tgaaaaagat tccgccgcgt tgcacaaaca gatgtttcgg agcggcattt
    16621 tgctacaaat ttcatttgaa atcaaagcct gtttgcaagt ttacaatcgt ttacccaaaa
    16681 aagggcaatt ttaccccgaa cctatttctt tagtattaga cctattatcc tttacttctt
    16741 aatattaacg gatgtttaca caaattcccg tatacatttt atgcgccatg ccttctaacc
    16801 aagtttgcca atgcctccgc caattcggga tgccgttttt ccaactttgc cgccgccgaa
    16861 ccgaaactct ccagcgcagc cttactcaaa tgcagggtat tggttttcgg cggtttttcc
    16921 ggtttcggga ccagcctgac cgaaacagag cgtatcgaag catcaagccc tgccaactgc
    16981 ggcaataccg acggtgcaat cattttcaag cgcgatgccg ccatattgtt tgccgccaaa
    17041 aggacaagcc tgccgtcttc gatacatgcc gtctgaaaat gcgggtgcag gttggcaggc
    17101 agcagttttt tcacggcggc atccaaccgc cgccactgtc ccgcctgttt caaaagtccg
    17161 gaaagcagcg cgtcccgcct gcccaactgt tccaaattca taaaacatac acccaaaaag
    17221 attgaaatac cgcaaacgcg cctttatttc agacggcatt agcactttgc acaaacgctt
    17281 gtgttaaaat cgcgttttcg cccactatta tatcaggcgc aggaattatt catgctgaca
    17341 aacattgcca agaaaatctt cggcagccgc aacgaccgct tgctgaaaca ataccgtaaa
    17401 tccgttgcca gaatcaacgc gctcgaagaa cagatgcaag ccctaagcga tgctgatctg
    17461 caagccaaaa ctgccgaatt caaacaacgc ctcgccgacg gtcagacttt ggacggcatt
    17521 ttgcccgaag ccttcgccgt ctgccgcgaa gcgtcccgcc gcaccctcgg tatgcgccac
    17581 ttcgacgtgc agcttatcgg cggtatggtg ctgcacgacg gcaaaatcgc cgaaatgcgt
    17641 accggcgaag gcaaaacctt ggtcgccacc ctcgccgtct atctcaacgc gctggccggc
    17701 aaaggcgtac acgtcgttac cgtcaacgac tacctcgcct cacgcgatgc gggcattatg
    17761 gagccgctct acaatttcct cggccttacc gtgggcgtga ttatttcaga tatgcagccg
    17821 ttcgaccgtc aaaacgccta tgccgccgat atcacctacg gcaccaataa tgaattcggc
    17881 ttcgactacc tgcgcgacaa tatggttacc gaccaatacg acaaagtgca gcgcgaattg
    17941 aattttgccg ttgtcgatga agtggattcc atcttgattg acgaagcgcg cactccgctg
    18001 attatctccg gtcaggcgga tgacaacatc cagttgtacc aaatcatgaa caccgttccg
    18061 ccccacctcg tccgtcaaga gacagaagaa ggcgaaggcg actattgggt cgacgaaaag
    18121 gcacatcagg tcatcctgag cgaagcaggt cacgaacacg ccgagcaaat cctgacccaa
    18181 atgggattgc tggcagaaaa cgactccctc tattccgccg ccaatatcgc cctgatgcac
    18241 caccttatgg cggcattgcg cgcgcattcc ctcttccaca aagaccaaca ttacgtcatc
    18301 caagacggcg aaatcgtcat cgtggacgaa ttcaccggcc ggctgatgtc cggccgccgc
    18361 tggtcggagg gtctgcatca agccgtcgaa gccaaagaag gcgtggaaat caaacgcgaa
    18421 aaccaaacgc ttgcatctat taccttccaa aactatttcc gcctgtacac caagctctcc
    18481 ggcatgaccg gcacagccga taccgaagcc ttcgagttcc aaagcatcta caacctcgaa
    18541 accgtcatca ttccgaccaa ccgccccgta cagcgcaaag acttcaacga ccagattttc
    18601 cgttccgccg aagaaaaatt cgaagccgtc gttaaagaca ttgaggaatg ccacaaacgc
    18661 gggcagcccg tcctcgtcgg caccaccagc attgaaaact ccgaactggt atccaagctg
    18721 ctgacccaag ccggactgcc gcacaacgtc ctcaacgcca aagaacacga acgcgaagcc
    18781 ctgattgtcg cccaagccgg caaagtcggc gcgattaccg ttgccaccaa tatggcggga
    18841 cgcggtacgg acatcgtttt aggcggcaac ctgaagcacc aaaccgatgc catccgcgcc
    18901 gacgaaacct tgagcgacga agagaaacag gcacaaatcg ccgcactcga agacggctgg
    18961 caggcggaac acgacaaagt gatggaagca ggcggtttgc acatcatcgg tacggaacgc
    19021 cacgaaagcc gccgcatcga caaccaattg cgcggacgtt ccggccgtca gggcgacccc
    19081 ggatccagcc gcttctatct ctcctttgaa gacccattgc tgcgcttatt cgcactcgac
    19141 cgcgccgccg ccatcctcaa ccgcctcgcc cccgaacgcg gcgtcgccat cgaacacaac
    19201 ctgctgacgc gccaaatcga aggggcgcaa cgcaaagtcg aaggcagaaa cttcgatatg
    19261 cgcaaacagg ttttggaata cgacgacgtt gccaacgaac agcgcaaagt catttacagc
    19321 cagcgcaacg aaattctgac cagcaaagac atcagcgacc tgatgcagga aatccgttct
    19381 gatgtcgtca gcgacctcgt ggatacctat atgccgcccg acagcatgga agaacaatgg
    19441 gacatcccga ctttggagaa ccgtctggct gccgaattca gactgcacga agacatccaa
    19501 tcctggctga aggcggacaa tgcgattgac ggtcaagaca tcaaagaacg cctgatcgaa
    19561 cgcatcgaaa acgaatatgc cgccaaaacc gaactggtcg gcaagcaggc aatggccgat
    19621 ttcgagcgca acgtgatgtt gcaggtcatc gacaaccaat ggcgcgaaca cctcgccgct
    19681 atggactacc tgcgacaagg catacacctg cgcagctatg cccaaaaaaa tccgaagcag
    19741 gaatacaaac gtgaagcctt taccatgttc caagacctgt ggaacggcat caaattccat
    19801 attgcctccc tgcttacctc ggttcaaatc gaacaaaacc ctgtcgcggt ggttgaagag
    19861 caacccatcg gcaacatcca gtccatccat tccgaatcgc ccgatatgga agaacttttg
    19921 ggtcagtcgc aaaccgatct ggttaccgaa gcctttaatc ccgatgggac agatttcagc
    19981 cccgaagcct tggaagcgcg ggggcaaatc gtccaccgca acgacccctg cccctgcggc
    20041 agcggtttga aatacaaaca atgccacggc aaactggctt aagcgtttga acgcaaatgc
    20101 cgtctgaaca tcccgctccc gtttcagacg gcattttgcc tgaaccgcca catccgactg
    20161 ccattccgaa aaatcccgat ttcgtaccgt ccgtaccaaa aacagacatc ccgtccgccc
    20221 cacatcatga ttccatccga cttcattgac gagcttttag ccaaaaccga tattgtcgat
    20281 attatcgacg agcaggttcc gctgaaaaaa ggcggggcga actatatggc gtgttgcccg
    20341 ttccacaagg aaaaaacgcc gtcgttttcg gtcagtccaa ccaagcagtt ttaccattgt
    20401 ttcagttgcg gggcacacgg ctcagcgatt ggttttgtga tggaacatca gggactgtcg
    20461 tttccggagg cggttcagtt ccttgccgac cgcgtgggta tggtcgtgcc gaaagtgcac
    20521 gggcaaaacg ataatcccga agtccgtgcc gaacgtaaga aaaaacagca gacactggag
    20581 gaaacgacgg ctgcggcagc tgatttttac gcgcaacagc taaaattcaa tccagcggca
    20641 aaagcttatt tggacaagcg cggcttgagt gcagaagtta tcgcgcatta tggtttgggc
    20701 tatgcgcccg acggctggca gcctttgacg caagtgttcc aaccgtatcc taataccgcg
    20761 ttagtggata cggggatggt gattgacaat gagggacggc attacgaccg cttccgccat
    20821 cggattatgt tccccatccg caatccgcgc gggcaggtta tcggtttcgg cggcagggtg
    20881 ctggacgact cgaagccgaa atatttaaat tctcccgata cgcctttgtt cgataagggg
    20941 aaaaaccttt acggactgta tgaagggcgt gccgctgtca aggaagcggg gcggattttg
    21001 gtggtcgaag gctatatgga cgtggtcgcg ctggcacagt tcggcgtggg ctacggcgtg
    21061 gcggctttgg gtacggcgac gacggcggaa cacgtcaaaa tcctgatgcg tcaggcagac
    21121 agtatttatt tctgtttcga cggcgacagc gcggggcgaa aagcggcttg gcgcgcgctg
    21181 gaaaacgcgc tgccgcagtt gaaggacgac aaatcgctgc attttttgtt cctgccggaa
    21241 gaacacgacc ccgacagcta catccgcgcc tacggcaaag cgcaatttga agacgcgctt
    21301 ctgaatcaaa gcaagccttt gtcggagtat ttctgggaac acctttcaga cggcattcat
    21361 ctcaatacgc aggaaggcaa ggcggaattg gtaaaaacca gttcgccgct tttggcgcag
    21421 attaccgcgc cggcattggc ttatttgtta aaacaacggc ttagcgagct ggtcggcatc
    21481 gaccccgaca acctcgcgca actgctagga caggaagcgc cgaagcggca cgtcaaacaa
    21541 aaaaactaca aactgcctcc gatttccgtc aaacagcccg tcatgctgac gctggtacag
    21601 cggcaaatcc gcagcctctt gataaatccg gattgggctg catatataga cctgcccgat
    21661 tatctggcgt tggacggtga tttcgcctgc cttgccaatc ttgccgaatc gattaaaaac
    21721 catgccgccg tacccgaaac cgctcaggtt ttagagtata tgcgcggctc gccttacgaa
    21781 gaaacgataa cccgaatctt ccattcaacg caccaatcgg aagaaatgaa cagcagcagt
    21841 gaagaagatt gcgagaattt ccaaatcggc atgaaaaaac tgctcaatga gttaaaatac
    21901 agccaaatcg aaacattaaa acaaaaaagc ctgcaatccg gcttaaatga aagcgagaaa
    21961 aaacttttgc tgtcgctgct gaccgcaaaa caaaattgac cggcggattc cgccatccgt
    22021 aaaccgttat gccgtctgaa aagcattcac cccggctgca acaacgacac ctgcagaaca
    22081 cccatcccca aaagccttca gacggcatca gagtacccta ctctgccacg ccttcaggtg
    22141 cgtccaaacg caaaccgtcg gcatcttacc aacagaaagc agacaatgtc cagaaaccaa
    22201 aatcacgaag aatatcaaga cgacacccgt ccgttaagca ttgaagagca acgcgcgcgc
    22261 ctgcgtcagc tcatcatcat gggtaaagaa cgcggctaca tcacctactc cgaaatcaac
    22321 gacgccctgc cagacgatat gtctgatgcc gaccaaatag acaatatcgt cagcatgatt
    22381 tccggtttgg gcatccaagt taccgaacac gcccccgatg cggaagacat attgttaagc
    22441 gacaatgccg ccgttaccga cgatgatgcc gtcgaagaag ccgaggccgc cctttccagt
    22501 gcagattccg agttcggcag aaccaccgac cccgtccgta tgtatatgcg cgaaatggga
    22561 caggtcgacc tgctgacccg cgaagacgaa atcatcatcg caaaaaaaat tgaaaacgcc
    22621 ctgaaaaata tggttcaggc catctccgcc tgcccgggat ccattgctga aatcttagaa
    22681 ctcatcgaaa aaatccgcaa agacgaaatc cgcgtcgacg aagtcgtaga agccattatc
    22741 gacccgaatg aagtattgct caacgaattg ggcttggggc acttggaaac cacagcgccc
    22801 gagaaacctt ccaacgacaa ttcggacgaa aacgaagacg acgaagaatc ggaagaagat
    22861 gcggatgaaa tctcggcagc caatctcgcc gaattgaaac aaaaagtcat cggccacttt
    22921 gcccaaatcg aaaaagacta caaaaaaatg attggccgtt tggaaaaaca ccacagccgg
    22981 cacaaagact atctcgccta ccgcgacgcg attgccaaca aactgctgga agtccgtttc
    23041 gccacccggc aaatcgacag cctcagcagc agcctgcgcg ggaaagtaga aaacatccgc
    23101 aaactcgaac gcgaaatccg cgacatctgc ctcgaccgcg tccatatgga acgcgactac
    23161 ttcatccaaa acttcctgcc cgaaatcacc aatctagaat ggattgaaga agaaatcgcc
    23221 aaaggcaggg tttggagcga cgcgctcgac cgcttccgcc acgccatcct cgaaaaacaa
    23281 accgagttgg cggatatgga aaaagaaacc cgcatttcca tcgaagagtt gaaagaaatc
    23341 aacaaaaata tggtgtcgag cgaaaaagaa accgcagccg ccaaacagga aatgattcag
    23401 gcaaacttgc gcctcgtgat ttccatcgcc aaaaaatata ccaaccgggg cttacaattc
    23461 cttgatctga ttcaggaagg caacatcggt ttgatgaaag cggtcgataa gttcgaatac
    23521 cgcagaggct ataaattctc cacctacgca acctggtgga tccgccaggc aattacacgc
    23581 tcgattgccg atcaggcgcg taccatccgc attccggtac atatgattga aaccatcaac
    23641 aagatgaacc gcatctcgcg ccaacacctt caagaaaccg gcgaagaacc cgattccgcc
    23701 aaacttgccg aactgatgca gatgcccgaa gacaaaatcc gcaaaatcat gaaaatcgcc
    23761 aaagagccga tttcgatgga aacccccatc ggcgacgacg acgattcgca cttgggcgac
    23821 ttcatcgaag atgccaacaa tgttgcgccg gccgatgcgg caatgtacac cagcctgcac
    23881 gaagtaacca aagaaatcct cgaaagcctg acaccgcgtg aggcaaaagt cctgcgtatg
    23941 cgtttcggca tcgatatgaa caccgaccac acgctggaag aagtcggcag acagtttgac
    24001 gtaacgcgcg aacgcatccg acaaatcgag gcaaaagcac tccgcaagct gcggcatccg
    24061 acaagaagcg accgtttgag aagtttcttg gacagcgaag acagcaagct gtaaaccaaa
    24121 aaaccgcagg tttcaaatac ctgcggtttt ttcttacaca ataaacaacg cttccacata
    24181 tcccacactc ctatcccgag acctttgcaa aattccccaa aatcccctaa attcccacca
    24241 agacatttag gggattttcc atgagcacct tctttcagca aaccgcacaa gccatgattg
    24301 ccaaacacat cgaccgtttc ccactattga agttggatca ggtaattgat tggcaaccga
    24361 tcgaacagta cctgaaccgt caaaaaaccc gttaccttcg ag
//
LOCUS       DAWGRZ010000011        23523 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000011 DAWGRZ010000000
VERSION     DAWGRZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23523
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.011"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            52..1728
                     /gene="pilB"
                     /locus_tag="WHT44_000311"
     CDS             52..1728
                     /gene="pilB"
                     /locus_tag="WHT44_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ2532548.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
     gene            complement(1813..2022)
                     /locus_tag="WHT44_000312"
     CDS             complement(1813..2022)
                     /locus_tag="WHT44_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ2532549.1"
                     /translation="MTESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(2015..2647)
                     /locus_tag="WHT44_000313"
     CDS             complement(2015..2647)
                     /locus_tag="WHT44_000313"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ2532550.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFISLIRRVLTISAPVEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHND"
     gene            complement(2649..3509)
                     /locus_tag="WHT44_000314"
     CDS             complement(2649..3509)
                     /locus_tag="WHT44_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ2532551.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLVLTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSLWLLCAVYKLLTGKTGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            complement(3583..4815)
                     /gene="pilG"
                     /locus_tag="WHT44_000315"
     CDS             complement(3583..4815)
                     /gene="pilG"
                     /locus_tag="WHT44_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ2532552.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            5172..6815
                     /gene="pgi"
                     /locus_tag="WHT44_000316"
     CDS             5172..6815
                     /gene="pgi"
                     /locus_tag="WHT44_000316"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ2532553.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCNLADAADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYVDGRDVLPEIRRELNRALKFAHSLDDGSYQGITGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHITVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDRHFFSTPTRHNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGREDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILIDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            7025..7846
                     /gene="dapD"
                     /locus_tag="WHT44_000317"
     CDS             7025..7846
                     /gene="dapD"
                     /locus_tag="WHT44_000317"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ2532554.1"
                     /translation="MSLQNIIETAFENRADITPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            8001..8786
                     /gene="fabI"
                     /locus_tag="WHT44_000318"
     CDS             8001..8786
                     /gene="fabI"
                     /locus_tag="WHT44_000318"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ2532555.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(9030..10028)
                     /gene="ilvE"
                     /locus_tag="WHT44_000319"
     CDS             complement(9030..10028)
                     /gene="ilvE"
                     /locus_tag="WHT44_000319"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ2532556.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETEAYLDALIK
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            10338..10652
                     /locus_tag="WHT44_000320"
     CDS             10338..10652
                     /locus_tag="WHT44_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ2532557.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIIFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESTK
                     QP"
     gene            10663..11832
                     /gene="lapB"
                     /locus_tag="WHT44_000321"
     CDS             10663..11832
                     /gene="lapB"
                     /locus_tag="WHT44_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ2532558.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDMNP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            11857..12273
                     /gene="gloA"
                     /locus_tag="WHT44_000322"
     CDS             11857..12273
                     /gene="gloA"
                     /locus_tag="WHT44_000322"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ2532559.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(12445..14799)
                     /locus_tag="WHT44_000323"
     CDS             complement(12445..14799)
                     /locus_tag="WHT44_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ2532560.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTV
                     AEVQTPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENITETV
                     AETPDFNATADDLSALLQPSKVPAVEENAAETVADDLSALLQPAEAPAVEENAAEITL
                     ETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNNADTSFPSESVGSDAPSEAK
                     YDLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            15107..15637
                     /locus_tag="WHT44_000324"
     CDS             15107..15637
                     /locus_tag="WHT44_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ2532561.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            15640..15930
                     /locus_tag="WHT44_000325"
     CDS             15640..15930
                     /locus_tag="WHT44_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ2532562.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            15930..16208
                     /locus_tag="WHT44_000326"
     CDS             15930..16208
                     /locus_tag="WHT44_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ2532563.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            16257..17123
                     /locus_tag="WHT44_000327"
     CDS             16257..17123
                     /locus_tag="WHT44_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ2532564.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            17194..17952
                     /locus_tag="WHT44_000328"
     CDS             17194..17952
                     /locus_tag="WHT44_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ2532565.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSEDELHKFYEQQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDALLEENGVKP"
     gene            18025..18516
                     /locus_tag="WHT44_000329"
     CDS             18025..18516
                     /locus_tag="WHT44_000329"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ2532566.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            18547..19557
                     /gene="dusA"
                     /locus_tag="WHT44_000330"
     CDS             18547..19557
                     /gene="dusA"
                     /locus_tag="WHT44_000330"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ2532567.1"
                     /translation="MNDNTHTLPPRHLSVAPMLDWTDRHYRYLARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVKIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANM
                     RE"
     gene            complement(19627..20514)
                     /gene="gluQRS"
                     /locus_tag="WHT44_000331"
     CDS             complement(19627..20514)
                     /gene="gluQRS"
                     /locus_tag="WHT44_000331"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ2532568.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARAHGGKWLIRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQAGARRGADGFVYNGRCRHPGQRPALQGKQPAWRIRVPDRIIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLDVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAVTAP"
     gene            complement(20531..20728)
                     /locus_tag="WHT44_000332"
     CDS             complement(20531..20728)
                     /locus_tag="WHT44_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532569.1"
                     /translation="MTKLVSRNASNERIADRAFDFFSPALMDGSATEEQYNALYDLTL
                     LEEPGMELNKDEIMALINSLK"
     gene            complement(20802..21161)
                     /locus_tag="WHT44_000333"
     CDS             complement(20802..21161)
                     /locus_tag="WHT44_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ2532570.1"
                     /translation="MINLELKAINLYDCAFEDFVPEIKDNFCVGIDLDIGTDDSDAAD
                     IFSVRICSPKWILHNCFQNQRVKWGAGMMIMNEFNHSVIKSEIEKILKECSKETWEKS
                     LTYLLRFFSWEFEDYQC"
     gene            complement(21405..22460)
                     /gene="tal"
                     /locus_tag="WHT44_000334"
     CDS             complement(21405..22460)
                     /gene="tal"
                     /locus_tag="WHT44_000334"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ2532571.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEIAALKQQNLSPKQRYETMAVADVRAACDVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIQVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKAAYQDWAQYFGSPEFAALETKGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLATRLQED
                     GLKQFEEAFEKLLAPLA"
     gene            22557..>23523
                     /locus_tag="WHT44_000335"
     CDS             22557..>23523
                     /locus_tag="WHT44_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ2532572.1"
                     /translation="MAENGKYLDWAREVLHAEAEGLREIAAELDKNFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAAR"
BASE COUNT         5314 a         6017 c         6679 g         5513 t
ORIGIN      
        1 ctataaaagc tgtacaggta taacaatgaa taaatttggg gataaggtcg tatgagcgta
       61 ggtttgctga ggattctggt tcaaaaccag gtggttactg ttgagcaggc cgagcattac
      121 tacaatgagt cgcaggcggg taaggaagtg ttgccgatgc tgttttcaga cggtgtcatt
      181 tcgcccaagt cgcttgcggc attgattgcg agggtgttca gttattcgat tcttgatttg
      241 cgtcattatc cgcgccacag ggtgctgatg ggggtgttga cggaggagca gatggtggag
      301 ttccactgtg tgccggtttt ccgtcggggc gacaaagtat tttttgcggt ttccgatccg
      361 acacagatgc cgcaaattca gaaaaccgtt tctgccgcag ggattgaggt tgagttggtc
      421 attgtcgagg atgaccagtt ggcgggtttg ctcgattggg tgggttcgcg ttcgacatcg
      481 ctgcttcagg agcttgggga ggggcaggag gaagaggaaa gccacaccct gtatatcgac
      541 aacgaggagg cagaagacgg ccctgttccg aggtttatcc ataagacttt gtcggatgcc
      601 ttgcgcagcg gggcatcgga catccatttc gagttttacg aacacaatgc ccgtatccgt
      661 ttccgtgtgg acgggcagct ccgcgaggtg gttcagccgc ccattgcggt aagggggcag
      721 cttgcttcac ggattaaggt aatgtcgcgt ttggacattt ccgaaaaacg gataccgcag
      781 gacggcagga tgcagctgac ctttcaaaag ggcggcaagc ctgtcgattt ccgtgtcagc
      841 acattgccga cgctgtttgg cgaaaaggtc gtgatgcgga ttttgaattc cgatgccgcg
      901 tctttgaaca tcgaccagct cggttttgag ccgtttcaga aaaaattgtt gttggaagcg
      961 attcaccgtc cctacgggat ggtgctggta accggtccga cgggttcggg taagacggtg
     1021 tcgctctata cctgtttgaa tattttgaat acggagtcgg taaatattgc aacggcggaa
     1081 gaccctgccg agattaacct gccgggcatc aatcaggtta acgtcaatga taagcagggc
     1141 ctgacttttg ccgctgcttt gaagtctttc ctgcgtcagg acccggacat cattatggtc
     1201 ggtgagattc gtgatttgga aactgccgat attgcgatta aggcggcaca aacagggcat
     1261 atggtgtttt ccaccctgca caccaataat gcgccggcga cgttgtcgcg tatgctgaat
     1321 atgggtgtcg cgccgtttaa tattgccagt tcggtcagcc tgattatggc gcagcgtctt
     1381 ttacgcaggc tgtgttcgag ctgcaaacag gaagtggaac gcccgtctgc ctctgctttg
     1441 aaggaagtcg gcttcaccga tgaggacctt gcaaaagatt ggaaacttta ccgcgccgtc
     1501 ggttgcgacc gttgccgggg gcagggttat aaggggcgtg cgggcgtgta tgaggttatg
     1561 cccatcagcg aagaaatgca gcgtgtgatt atgaacaacg gtacggaagt ggatattttg
     1621 gacgttgcct ataaggaggg tatggtggat ttgcgccggg ccggtatttt gaaagttatg
     1681 cagggcatta cttcattgga agaggtaacg gcaaatacca acgattaggt ttgagaatga
     1741 aaatgccgtc tgaagcgtgt ttgtttcaga cggcatttga ctttcagggt gtttgccggg
     1801 aaggcggggc ggtcagcggt atgccatgtc gggttcggat atttccggca aactttccgt
     1861 ttggccggaa accgtatatt tcccgtctgc ccatccgccc aagtcgatca gtttgcagcg
     1921 ttgcgaacag aaggggcgga atgcgttttc gggtttccat actactgctg tttgacaggt
     1981 cggacatttg acttgaaggc gtgtttgccg cgattcagtc attgtgtttt ccttgtgttg
     2041 gttttgaggc gaaaatccct gaataaaacg cgtgcaggcg cattgttttc tcacgcaggc
     2101 ttttgaggct gccgtcattg agcagcacat cgtctgcaag cagcaggcgt tcggattcgg
     2161 atgcctgatg gctgatgacg gccgccacct cgccgcgcgt cagcccgctg cgggccatca
     2221 ccctgccgat acgtttttcc acaggggcac ttatggtcag gacacgccgt atcaggctga
     2281 taaattgacg cttttccgtc agcagcggaa tttcgacaat gccgtaagct gcatcagtaa
     2341 aggtttcttg ctgttttttg atttctgaga aaatcagcgg caacatcacg gattcgagca
     2401 aggcttttcg cgatggggag gcaaagactt ctttacgcaa tatgtcgcgc cgcaacaaac
     2461 cctgtgtgtc aaaaacggtg tcgccgaaca gccgcctgat ttccggcagg gcgatgccgt
     2521 ctgaagccgt cagcgagtgc gccgccgcgt ctgcatcgat gcgcggcacg cccaaatcgg
     2581 caaaacattg cgcggctgcc gatttgccgc tgccgattcc gccggtcagt ccgacccata
     2641 ccgtcatctt acagcaccgg atgggtcagc caccagttga ccgcccgcca tacggaatcg
     2701 tttgccgtaa aaattatcca gcccgaaact gtcagtgcgg ggccgaaggc aaaatgctgc
     2761 cccttggcga cgcgcataac gattgccgcg accaaaccga tcagcgagga aacaaaaatc
     2821 agtacgggca atgcggatat gccgagccac gcgcccaatg cggcaatcag tttgaaatct
     2881 ccgttgccca taccggtttt tcctgtgagc agtttataca ctgcacataa gagccataat
     2941 gaaccatagc cggcgaccgc acctaaaacg gcagactgca aaggcacgaa gccgccgtcc
     3001 aaattaaata tcagacccag ccaaattaag ggcagtgtca tcgagtcggg caggtattgg
     3061 gtgtccgcat cgataaaggt cagggaaatc agaaacgcgg tcagtaccaa tccgcccagc
     3121 gtaatccaag accagccgta ttgccaggcg accagcccga acaatacgcc ggtcagcagc
     3181 tcgattaagg gataacgtat gctgattttg gtttggcagg aagcgcattt gccgcgcagg
     3241 agcaggtagc tgacaatcgg gatgttctgc cacgcgcgta tcggcacgcg gcatttggga
     3301 cagcaggaat ccggtttcat caggttgaag gtacggcttt cctcttcggt cagcggcagg
     3361 tttaaatatt ctttggcaaa taccgtccag ccgcgttcca tcatgaccgg cacgcggtaa
     3421 atgacgacat ttaagaaact tccgaccagc agcccgaaca ccgctgccaa aggcacggca
     3481 aacggcgaca atacagacaa atcagacata ttttgttctc aatgtattca aaacaaaaac
     3541 aaaccggcgc agagcgaatc cgcgccggat ctgtgcggca aatcaggcga ccacgttgcc
     3601 caaattaaac agcggcagat acatggcgac cagaagcgtg ccgatgacca agcctaaaat
     3661 cacgataatg atcggctcca tcatagcgga cagcctgccg accgcattgt ccacctcgtc
     3721 ttcgtaaaat tcggcggctt tgttgagcat atcgtccaaa gaacccgatt cctcgccgat
     3781 ggaagacatc tgcaacatca tattggggaa cagttccgtc gcacgcatcc ccgaagtcat
     3841 agacaaacct tggatgacgc gcgtacggat ttcccgggtg gcttcttcat agattaaatt
     3901 gcccgccgcg ccggcagtgg agtccaatac atcgaccaaa ggcacgcctg ccgcaatcag
     3961 cgtcgccgtc gtcctgcccc agcgggcaat cgttcctttg cggacaatgt ctccgaaaat
     4021 cggcatacgc agcagtatgg catccatacg ccgttggatt ttaatcgaac gcgccttcaa
     4081 tttaaggaag ccgtatatgg caaagcccag tgcgatcagc accatccagc cgtatgagac
     4141 gaaaaagtcg gacatatcca tcactgtttg ggtcagtgcg ggaagctccg cgcccatatt
     4201 ggcgtaaact tctttaaagg cgggcagtac gaaaatcatc atcacgaata ccaaaccgat
     4261 ggcgacggcg atgacggata ccggataggt cagtgcggtt tttacctttt tgcggatggc
     4321 ctgggttttt tctttgtaaa ttgccaattt gtccagcagg ctttccaata cgccgcccgt
     4381 ttcgcccgcc gcaaccagat tgcagtagaa gcggtcgaaa tattttgggt ggtttgagaa
     4441 tgcgcggctc aacgagctgc cctgttccac ttcgcctcgg atttccatca gcatttccgt
     4501 catagacggg ttgccgtgtc cgcgcgccac gatttcaaat gcctgcatca gcggcaggcc
     4561 cgctttaatc atcgtggaca gctggcgggt gaaaacggtg atgtcttctt gtgtgatttt
     4621 gcgcttggag cttgttttca cacgggtaat ctgcaacggg cggatgccgc gttttgccag
     4681 ttttttgcgc gcctcttctt cggtaaacgc ggatacttcg ccgttgacca gtttgtcgga
     4741 ggcggaatgc ctgccttcaa agataaagcg tttttctttc tttgcgaaca aagaaaatcc
     4801 tccgttttta gccatattct agccccgtaa agtaattgga ataaaatgta agaaacatcg
     4861 ttaaaaaaca gtaccggcgt gttcccggta agatgaaaac cgccgacatc ccgcctgcgg
     4921 gcggcaaacg ggacagaatc ggatgcgatt ataccttatt taggcggctg tccggcattt
     4981 atgcgtacac aataaatctt gcaggatatt gttgcgggtc aaatgccggc cggagggcat
     5041 ttccgccata tggaaataag gtgctattgg acgcggcggg cggtgttccg gagattcgcc
     5101 aaagccgctg ccgtttgtta aactacattc tgctacattt taatccggtt ctgaaaaatc
     5161 aaggaaaaca gatgaatgct tttacccgtg catggtatgc gctcgaacgc cattatcagg
     5221 atacgcgtca tgtccttttg cgcgaccgct ttgcctgcga accggaccgg tttgagcgta
     5281 tgcacgagcg tttggacggg atgttgttcg attacagcaa aaaccgtttg ggcgaagata
     5341 cgctgcaact gctctgcaat cttgccgacg cggcggattt ggaagggaaa atgcgtgctt
     5401 tgcggacggg tgcgaaagtc aacggcagcg aggggcgtgc cgcgctgcat acggctttgc
     5461 gcctgcccga cggtgcggat gccgtttatg tggacggcag ggacgtgttg cccgaaatcc
     5521 gccgcgagtt aaatcgtgcg ttgaagtttg cacacagttt ggacgacggt tcgtatcagg
     5581 ggataaccgg aaaacggatt acggattttg tccacatcgg cataggcgga tccgacctcg
     5641 ggccggcaat gtgcgtgcag gcacttgagc cgttcagacg gcatatcacc gtccattttg
     5701 ccgccaacgc cgatcctgcc tgcctggatg cggttttatg ccgtctgaac cccgaaacga
     5761 cagtgttttg cgttgccagc aagtccttca aaacaccgga aaccctgctc aatgcacagg
     5821 cagtcaaggc gtggtatcgc ggtgcagggt tctcggaatc cgaaacggcg tgccattttt
     5881 gcgcggtgtc tgccgacact gcggcagctg cggcttttgg tatcgcggcg gaacgcgtgt
     5941 ttgcgatgta cgactgggtg ggcggacgct attccgtctg gtcgcccgtc ggtttgcccg
     6001 tgatggttgc ggtcggcggg gcgcgtttcc gcgagttgtt ggcgggggcg cacgcgatgg
     6061 acaggcattt tttcagtacg ccgacgcgtc ataatatccc cgttttaatg gcactgattg
     6121 ccgtgtggta caacaatttc cagcacgcgg acgggcagac cgccgttccg tacagccaca
     6181 acctgcgcct gctgccggcg tggctgaacc agctcgatat ggagagtttg ggcaaaagcc
     6241 gcgcttcaga cggcagtccc gccgtgtgca aaacgggcgg catcgtgttc ggtggtgaag
     6301 gggtcaactg ccagcacgcc tatttccaac tgctccacca aggcacgcgc ctgattccct
     6361 gcgattttat cgtcccgatg acggcgcagg gcagagagga cggacgcagc cgttttaccg
     6421 ttgccaacgc ctttgcccaa gcggaagcct tgatgaaggg caaaaccttg gacgaagcac
     6481 gcgccgaact ggcagatttg cccgaagcgg aacgcgaacg cctcgcgccg cacaaagagt
     6541 tccccggcaa ccgccccagc aacagcattt tgattgaccg cctcacgccc tacaatttgg
     6601 gtatgctgat ggcggcttac gaacacaaaa ccttcgtcca aggcgcgata tggaacgtca
     6661 accccttcga tcagtggggg gtggaatacg gcaaacagtt ggcaaaaacc atcatcggcg
     6721 aactggaagg cggcacgtcc gtacacgatg cctcgaccga agggctgatg gcgttttacc
     6781 gcgaatgccg tctgaaaggc ggcggcgcgg cataaaagta ctgccgcctt tctgtattga
     6841 ttcgggcgcg gaaaaggcaa tacctgccgc ctgcccgatt ccgaaacgcc aatgtttggc
     6901 aaccgctcgc gtattgctga cgaatatgcg tttgcgtggc acaatagcgc attcatttca
     6961 aatgaacata ctgcttgaaa ataccggcaa gcgtcccacg aaacatctca cataaggaaa
     7021 tattatgtct ttgcaaaaca ttatcgaaac cgcctttgaa aaccgcgcgg acatcacccc
     7081 gaccaccgtt actcccgaag tcaaagaagc cgtgttggaa accatccgcc aactcgattc
     7141 cggcaaactg cgcgttgccg aacgtttggg cgtgggtgag tggaaagtca acgaatgggc
     7201 gaaaaaagcc gtgttgctgt ccttccgcat ccaagacaac gaagtcctca acgacggcgt
     7261 gaacaaatac ttcgacaaag tgccgaccaa gtttgccgac tggtctgaag acgagttcaa
     7321 aaacgcaggc ttccgcgcag ttccgggtgc ggttgcccga cgcggcagct ttgtggcgaa
     7381 aaatgtcgtg ctgatgccat cttatgtcaa catcggcgca tacgtcgacg aaggcgcgat
     7441 ggtcgatact tgggcaaccg tcggctcttg cgcgcaaatc ggtaaaaacg tgcacttgag
     7501 cgggggcgtc ggcatcggtg gtgtactcga acccctgcag gccgcaccca ccatcattga
     7561 agacaactgc ttcatcggtg cgcgttctga aatcgttgag ggcgtgattg tcgaagaagg
     7621 cagcgtgatt tctatgggcg tgttcatcgg tcaatccacc aaaatctttg accgtacaac
     7681 cggcgaaatc tatcaaggcc gcgtaccggc aggttcggtt gtcgtatccg gcagtatgcc
     7741 ttccaaagac ggcagccaca gcctttactg cgccgtcatc gtcaaacgcg tggacgcgca
     7801 aacccgtgcg aaaaccagcg tcaacgaatt gttgcgcggc atctgatgcc ttaaaccgta
     7861 tttgaaacgt ccaatgccgt ctgaaatccg cttcagacgg cattgccgtt tgcacgctgc
     7921 aacgtgaaaa cacagaaaca gggacaattt gctataatca acggtttaga acgaaccgaa
     7981 cactatttga aggatacaaa atgggttttc tgcaaggcaa aaaaattctg attaccggca
     8041 tgatttccga gcgttccatc gcttacggca tcgccaaagc ctgccgcgaa caaggcgcgg
     8101 aactggcgtt tacctacgtt gtggacaaac tggaagagcg cgtccgcaaa atggcggcgg
     8161 aattggattc cgaacttgta ttccgctgcg atgtcgccag cgacgacgaa atcaaccaag
     8221 tgttcgccga cttgggcaaa cattgggacg gcttggacgg tttggtgcat tccatcggtt
     8281 ttgcgccgaa agaagccttg agcggcgact tcctcgacag catcagccgc gaagcgttca
     8341 acaccgcaca cgaaatttcc gcatacagcc tgcccgcgtt ggcaaaagcc gcccgtccga
     8401 tgatgcgcgg cagaaattcc gccatcgtcg ccctgagcta cttgggcgcg gtgcgcgcga
     8461 ttccgaatta caacgtgatg ggtatggcaa aagccagcct tgaggcaggc atccgcttta
     8521 ccgctgcctg tctgggtaaa gagggcatcc gctgcaacgg tatttccgcc ggcccgatta
     8581 aaacgcttgc cgcctccggc atcgccgatt tcggcaaact cttgggacac gtcgccgccc
     8641 acaacccgct ccgccgcaac gttaccattg aagaagtcgg caataccgcc gccttcctgc
     8701 tgtccgacct gtcgtccggc attaccggcg aaatcactta cgttgacggc ggttacagca
     8761 ttaatgcctt gagcaccgag ggataatccg ccgttttcaa atccgtgcgc cgtccgtgcc
     8821 gcatatcggt ttcgggcggc gttttgccgt ctgaagcgta tttctaggga aatgcccgac
     8881 ttacggcagg cgggatggga aatgcggacg cttgttttaa ccgattgcct ttgtgccgac
     8941 ttgctgcagg tgcagcggaa acggttcgga tgcgaaaatg ccgtctgaaa cgccaaacgg
     9001 gtttcagacg gcatttttta tttaaagcat cagcacactt caaccagcca gccgtatttg
     9061 tcttccgcca aaccatactg gatgtcggta atcgccttac ggatggcata gccgcgttct
     9121 tggcttttca cttcgatttc tttgccgccg atgacgaagg aagtaacggg cgagatgacg
     9181 gctgccgtac cggtcaaaat ggcttccgca ccgttttcca ccgcagcttt gagttcgtca
     9241 accgtgaaat tgcgttcgct gacggtatag cccaaatctt tggcaaccgt cagtacggaa
     9301 tcgcgggtta cgccgtgcaa aaactcgtcg gtcagcggtt tggtaatgat ttcatcgccg
     9361 ttaatcagga taaagttgga cgcgccggtt tcctgcacgt cgccgttcgg gcagaacagg
     9421 acttgatttg cgccatattc ggctttcgcc ttcagcaccc agtgcatggc ggaagcgtag
     9481 ttgccgccgc atttgacgcg gcccatatgc ggggcgcagc ggatgtgttc ggtttccacc
     9541 aaaattttga cgggcgatcc gactttgaaa tagtcgccga cgggggaagc caaaatatac
     9601 agcagggcgg tttcggaagg agaaccggcc ttgccgataa cgggatcggt accgattaag
     9661 gtcggacgca ggtacagggc ggcaggcgca tcgggaattt catcggcggc acgtttgacc
     9721 aatttgatta gcgcgtcaag ataagcttcg gtttcggggc gcggcaggtg caaaatgtcc
     9781 gcactttgcc gcatacgcgc gatattggca gtcggacgga acagcacgat tttgccgtct
     9841 gcctgacgga aggctttcag tccctcgaaa cattcgctgc cgtagtgcag ggcgtgcgcg
     9901 cccggtgcga gggagaggtc ttgggaagat tgccattcgg tcggctgcca tttgccttcg
     9961 cggtaggcga ggacgggcat ttgactgtga aaaacgctgc cgaatacggc gggtacgggt
    10021 ctgctcatga tgtaaagcct ttcttattct gatatgtttc aatgaacggt ttgaatttga
    10081 agattgtaaa gatacgcctg caaacagggt tttgacaagt gcgcggcggg tttttctgtc
    10141 gatgcggtgt ccaatccgtt atttttcaaa tggaaaggaa cggtgtattt ggtaaaattg
    10201 tcggcaatcg catactccgt atgtcgtccg aacacgctgc cgcatcctat ccgaaaccgt
    10261 gcaaatcgtt taaactagcg caatcttggt tcagagtgcg aagctgtctg ggcggcgttt
    10321 ttatttacgg agcaaacatg aaacttatct ataccgtcat caaaatcatt atcctgctgc
    10381 tcttcctgct gcttgccgtc attaatacgg atgccgttac cttttcctac ctgccggggc
    10441 aaaaattcga tttgccgctg attgtcgtat tgttcggcgc atttgtagtc ggtattattt
    10501 ttggaatgtt tgccttgttc ggacggttgt tgtcgttacg tggcgagaac ggcaggttgc
    10561 gtgccgaagt aaagaaaaat gcgcgtttga cggggaagga gctgaccgca ccaccggcgc
    10621 aaaatgcgcc cgaatctacc aaacagcctt aagaaagccg atatggacaa cgaattgtgg
    10681 attatcctgc tgccgattat ccttttgccc gtcttcttcg cgatgggctg gtttgccgcc
    10741 cgcgtggata tgaaaaccgt attgaagcag gcaaaaagca tcccttcggg attttataaa
    10801 agcttggacg ctttggtcga ccgcaacagc gggcgcgcgg caagggagtt ggcggaagtc
    10861 gtcgacggcc ggccgcaatc gtatgatttg aacctcaccc tcggcaaact ttaccgccag
    10921 cgtggcgaaa acgacaaagc catcaacata caccggacaa tgctcgattc tcccgatacg
    10981 gtcggcgaaa agcgcgcgcg cgtcctgttt gaattggcgc aaaactacca aagtgcgggg
    11041 ttggtcgatc gtgccgaaca gatttttttg gggctgcaag acggtaaaat ggcgcgtgaa
    11101 gccagacagc acctgctcaa tatctaccaa caggacaggg attgggaaaa agcggttgaa
    11161 accgcccggc tgctcagcca tgacgatcag acctatcagt ttgaaatcgc ccagttttat
    11221 tgcgaacttg cccaagccgc gctgttcaag tccaatttcg atgtcgcgcg tttcaatgtc
    11281 ggcaaggcac tcgaagccaa caaaaaatgc acccgcgcca acatgatttt gggcgacatc
    11341 gaacaccgac aaggcaattt ccctgccgcc gtcgaagcct atgccgccat cgagcagcaa
    11401 aaccatgcat acttgagcat ggtcggcgag aagctttacg aagcctatgc cgcgcaggga
    11461 aaacctgaag aaggcttgaa ccgtctgaca ggatatatgc agacgtttcc cgaacttgac
    11521 ctgatcaatg tcgtgtacga gaaatccctg ctgcttaagt gcgagaaaga agccgcgcaa
    11581 accgccgtcg agcttgtccg ccgcaagccc gaccttaacg gcgtgtaccg cctgctcggt
    11641 ttgaaactca gcgatatgaa tccggcttgg aaagccgatg ccgacatgat gcgttcggtt
    11701 atcggacggc agctacagcg cagcgtgatg taccgttgcc gcaactgcca cttcaaatcc
    11761 caagtctttt tctggcactg ccccgcctgc aacaaatggc agacgtttac cccgaataaa
    11821 atcgaagttt aaccaccacc gaaaggaaca caaaaaatgc gcttactcca tactatgctc
    11881 cgcgtgggca atctcgaaaa atccctcgat ttctaccaaa acgttttggg tatgaaactg
    11941 ctccgccgaa aagattatcc cgaaggcaga tttacccttg ccttcgtcgg ttacggcgat
    12001 gaaaccgaca gcacggtttt ggaactgacg cacaactggg atacggaacg atacgacttg
    12061 ggcaacgcct acggacacat cgcggttgaa gtggacgatg cctacgaagc ctgcgaacgt
    12121 gtgaagcggc agggcggaaa cgtcgtccgc gaagccggcc cgatgaaaca cggcacaacc
    12181 gtgatagcct tcgtcgaaga ccccgacgga tacaaaatcg agttcattca aaagaaaagc
    12241 ggcgacgatt cggttgccta tcaaactgcc tgataccgcc gccgccaatg ccgtctgaag
    12301 cctttagggg tttcagacgg cattttgttg ccgtcgacct gctgtttgag cctgtgccgg
    12361 ttcaaacttt atccgttaca ccgataaggc aaaaaagatg ccgtctgaaa cggcatcctt
    12421 gatctgcgaa agggcagttg ggaatcaaat acccaattcc tgcgccaatg cttgggcacg
    12481 tttgagtacg tcgccttccg cttcttccag caatttctgc actgtctcgg cagcggcatc
    12541 gcggtcgccg atttcgagat acatttcggc aaggtcgtat ttcgcttcgg aaggcgcgtc
    12601 agaacctaca gattccgaag ggaaactggt atctgcatta tttgggatat tttcttccga
    12661 gaggtagatg ctccaatcta ccgtttcctc ctcgccgtct ttcaggaagt cgggcaaagc
    12721 gtctgcctca gaggtgttgg aatcaggcgt ttccaaagtg atttccgctg cattttcctc
    12781 aacggccggt gcttcagcag gttgcaacag tgcggacaaa tcatcggcaa cggtttccgc
    12841 tgcattttcc tcaacggcag gtactttaga aggttgaagt aatgcggaca aatcgtctgc
    12901 ggtggcgttg aagtcgggtg tttcggcaac ggtttccgtt atattttcct caacggacgg
    12961 tgcttcggca ggttgaagca atgcggacaa atcgtctgcg gcggcgttga aatcgggcgt
    13021 ttcaggcgca gtttccgcga cggcatcggt ttcgtacact ttcaggaaat cgtgcaactc
    13081 ttccggtgtt tggacttcgg caactgtttt ttccaagatg gtttcgggcg aggaagcctt
    13141 caggaagcct gccagtccgg agggtgaggc aggttttgcg gaagctgttt cttctgtgcc
    13201 gatatggttg tttgagggca ggttgtcgga gaaatcggta tcgacggttt ccggtttgtt
    13261 ttcggcagtt tgggcgacag attccggttc gggcgtgtcg atgacgattt cgacagggtt
    13321 gtacgggttg aaggtctcgg gctcgtacac gctgtctgtg gattcgatgg cgttccaatc
    13381 ggcatccgcg cgtttttggg tttcttcatc ctgcgtaagt gcgccggata aaatgccgtt
    13441 ttgcgcggct gccaggctgt cgaaatccaa gtcgatgcgg ttggaaggcg tatcggtttc
    13501 gacatcgaac gtttgttttg ccgataactc ttcttcagat tccccatcta aggcaagtgt
    13561 gtcgtttaca tcgtttttcg gagcgggttc gggcgttgcc ggagtttcga cttcggcaaa
    13621 ggtgatttct atgccgtcgt ctgccgcgtc gtcaaggtca ggctcttcct cagggacgga
    13681 ttcttcggta cggcgcgcgc gtttggattg ggcaaggcgc aaaagcagca gcagggcgat
    13741 taatgccgcg cctccgccgg caagcagcaa ggtgtacgaa ccgccgaaca gaccgtcaaa
    13801 cagtccgctt tcggtttctt cttcggcaga aacctgttcg acaggttcgg aaacggcgtt
    13861 accggtttcg tcggtcggcg tgtcgatggc agaagcggcg gcttcttggg gggcggattc
    13921 ggcagcggtt tccgatgcgg cagtatttgc agcgggtaca ggttcgggtc gaacggccgg
    13981 tttttccgct tttgcttcgg gcgcggcaac ttttgcttca ggtttttcaa ccggtttctc
    14041 taccgttgcc tgtttggacg gttcggacgg catggatgcg gtttcggctt tgggtttcgc
    14101 cgtttgcggt ttgggttgtt ccgctttgat cctgttcaga ttcggaatgt gaagcacgct
    14161 gcccgcacgc agtctgccgt gtgcggaaac atttgggttt gccttcagca gcgcatcggc
    14221 aacctgttcg agcgtcaggt gtttcgggcg gatggcggcg gcaatctgtt tgaccgtttc
    14281 gcctttgcgg acggtatggg ttttgccgtt gtatgccggt ttgacggctg cgttcgcgct
    14341 gtctttttta tcggttttgc ggagggcttt ggcgttttga ttttcttggg actctgctgt
    14401 cggagcggtt ttgcggtgtg tcttgccgtc tgaaagtgca gatttggttt tgggcgagta
    14461 gccgacagga tcgaggatgg cggtgtattc gcgtacctgt gcgcctgcgc cgatgcggaa
    14521 caccaggacg ggatcgcgga ctgcctgttc ggaagaaacg gcaatgacgg ctttgtcgcc
    14581 caacttgtgg actttggcgg tcaggccttt ttcggaaacg gtaacgctgc cgccgcctag
    14641 cagggctttg gcttcttcgc cggttacggt aatgctgccg gaaaagggtt cgtcaaggtt
    14701 ggactggata ttcagtccgc ccagtccagc atgtgcctga aaggatgcgg caactgcgac
    14761 ggaggcggca atcagtttga tttgtctgtt gtttttcaag atgtatcccc tgtgggttgg
    14821 cggctgaata cggtttgacc gcgtacagtc tgtaaatttc gtcatcatcg ggcatcggcg
    14881 gggcagtcgg ccggcgggca tttaatatgt gaatgtaccg accgccgcca cattttaaac
    14941 ggcaatcatt cgccgttttt acaaattatg acatatctcc atcttttttc aaaaacatct
    15001 gtgcatattt gcatcaatca aaacaaaatt tgttggtttt gcaggtgcaa aaacagggtt
    15061 ctgcctgtat gattagcgtt tatttgattt gctttctcat ttggatatga aattcgtcag
    15121 cgaccttttg tccgtcatcc tgtttttcgc cacctatacc gttaccaaaa acatgattgc
    15181 cgcaacggcg gtcgcattgg ttgccggtgt ggttcaggcg gcttttctgt attggaaata
    15241 taaaaagctg gatacgatgc agtgggtcgg attggtgctg attgtggtat tcggcggcgc
    15301 aaccattgtt ttgggcgaca gccgcttcat tatgtggaag ccgagcgttt tgttttggct
    15361 gggcgcgctg ttcctgtggg gcagccacct cgccggtaaa aacggcttga aggcgagtat
    15421 cggcagggag attcagcttc cggatgccgt atgggcgaaa ttgacgtata tgtgggtcgg
    15481 tttcctgatt tttatgggta tcgccaactg gtttgtgttt acccggttcg agtcgcaatg
    15541 ggtcaactat aaaatgttcg gctcgactgc actgatgctt gttttcttta ttattcaggg
    15601 tatttatctg agtacctgtc tgaaaaagga ggattgactg tggaatattt tatgttgctg
    15661 gcaacagacg gggaggatgt gcacgaggcg cgtatggcgg cacgtcccga acacctcaaa
    15721 cggctggaga cgctgaagtc ggaaggccgg ctgttgacgg caggcccgaa tcctttgccg
    15781 gaggactcca accgcgtttc gggcagtttg attgtggcgc agttcgagtc tttggatgcg
    15841 gcgcaggctt gggcggaaga cgatccctat gttcatgcag gcgtgtacag cgaagtgctg
    15901 atcaagccgt ttaaagcggt gttcaaataa tgccggccgt cgatttgatc cgcgaacgcc
    15961 tgcagacgct cgatccgctg gtgttggaaa tcggcgatga gagccatctg cacaaaggac
    16021 acgcgggcaa taccggcggc ggacattatg ccgttttggt cgttagcggc cgttttgaag
    16081 gcgtaagccg cctgaaccgc cagaaaacgg tcaaatcgct gctcaaagat ttgttttcag
    16141 gcggcatgat tcacgcgctc ggcatccggg cggctacccc tgacgagtat ttccatacgg
    16201 cggactgaat gaagtctgcc cgaacatttc aatttaaaat ttaaagagag aagattatga
    16261 aagcaaaaat cctgacttcc gttgcactgc ttgcctgttc cggcagcctg tttgcccaaa
    16321 cgctggcaac cgtcaacggt cagaaaatcg acagttccgt catcgatgcg caggttgccg
    16381 cattccgtgc ggaaaacagc cgtgccgaag acacgccgca actgcgccaa tccctgctgg
    16441 aaaacgaagt ggtcaatacc gtggtcgcac aggaagtgaa acgcctgaaa ctcgaccggt
    16501 cggcagagtt taaaaatgcg cttgccaaat tgcgtgccga agcgaaaaag tcgggcgacg
    16561 acaagaaacc gtccttcaaa accgtttggc aggcggtaaa atatggcttg aacggcgagg
    16621 catacgcatt gcatatcgcc aaaacccaac cggtttccga gcaggaagta aaagccgcat
    16681 atgacaatat cagcggtttt tacaaaggta cgcaggaagt ccagttgggc gaaatcctga
    16741 ccgacaagga agaaaatgca aaaaaagcgg ttgccgactt gaaggcgaaa aaaggtttcg
    16801 atgccgtctt gaaacaatat tccctcaacg accgtaccaa acagaccggt gcgccggtcg
    16861 gatatgtgcc gctgaaagat ttggaacagg gtgttccgcc gctttatcag gcaattaagg
    16921 acttgaaaaa aggcgaattt acggcaacgc cgctgaaaaa cggcgatttc tacggcgttt
    16981 attatgtcaa cgacagccgc gaggtaaaag tgccttcttt tgatgaaatg aaaggacaga
    17041 ttgcgggcaa ccttcaggcg gaacggattg accgtgccgt cggtgcactg ttgggcaagg
    17101 caaacatcaa acctgcaaaa taattctgaa aacgggatat ggcggcaaga cgttcagaca
    17161 ggcgttttgc cgccgcgcag gacagggaat accatgaaac agaaaaaaac cgctgccgca
    17221 gttattgctg caatgttggc aggttttgcg gcagccaaag cacccgaaat cgacccggct
    17281 ttggtggata cgctggtggc gcagatcatg cagcaggcag accggcatgc ggagcagtcc
    17341 caaaaaccgg acgggcaggc aatccgaaac gatgccgtcc gccggctaca aactttggaa
    17401 gttttgaaaa acagggcatt gaaggaaggt ttggataagg ataaggatgt ccaaaaccgc
    17461 tttaaaatcg ccgaagcgtc tttttatgcc gaggagtacg tccgttttct ggaacgttcg
    17521 gaaacggttt ccgaagacga gctgcacaag ttttacgaac agcaaatccg catgatcaaa
    17581 ttgcagcagg tcagcttcgc aaccgaagag gaggcgcgtc aggcgcagca gctcctgctc
    17641 aaagggctgt cttttgaagg gctgatgaag cgttatccga acgacgagca ggcttttgac
    17701 ggtttcatta tggcgcagca gcttcccgag ccgctggctt cgcagtttgc cgcgatgaat
    17761 cggggcgacg ttacccgcga tccggtcaaa ttgggcgaac gctattatct gttcaaactc
    17821 agcgaggtcg ggaaaaaccc cgacgcgcag cctttcgagt tggtcagaaa ccagttggag
    17881 cagggtttga gacaggaaaa agcccgcttg aaaatcgatg cccttttgga agaaaacggt
    17941 gtcaaaccgt aatggcattt ccaataccga tgccgtctga agcctttcag acggcattgc
    18001 acgttcaggt aaggaggacg gcttatgcgt gcggtcatac agaaaacggt aggtgcaaag
    18061 gtggatgtcg tgtccgaagc cggcacggaa acctgtggca aaatcgacgg cgggtttgtc
    18121 gtgttactcg gcgtaacgca tagcgacaca gaaaaagatg cacgctatat cgccgacaaa
    18181 atcgcccatt tgcgcgtgtt tgaagacgaa gcgggcaagc tgaacctgtc tttgaaagat
    18241 gtcggcggcg cggtgctgct ggtgtcgcag tttacgcttt atgccgacgc ggcaagcggg
    18301 cggcggcctt cgttttccca agccgcacct gcagaacagg cgcagcagct ttacctgcga
    18361 acggcggaac tgttgcgcgg acacgggatt catgtcgaaa cagggcgttt ccgcacgcat
    18421 atgcaggtgt cgctctgcaa cgatgggccg gtaaccatac tgctggactc tttcatgacg
    18481 cggatttccc caaaaatgaa ggttgttccg gattgaaatt gaatccgcaa tgataaaata
    18541 tcgacaatga acgacaatac acacaccctt cccccgcgcc acctgtccgt cgcccccatg
    18601 ctcgactgga cggacaggca ctaccgttac cttgcccgcc agattacccg aaatacttgg
    18661 ctgtacagcg aaatggtcaa tgccggtgcg attgtttacg gcgacaaaga ccgctttttg
    18721 atgttcaacg aaggcgagca gcccgtcgcc ctgcaactgg gcggcagcga tccgtccgat
    18781 ttggcgaaag ccgccaaagc cgccgaggca tacggttaca acgaggtcaa cctcaactgc
    18841 ggctgcccca gtccgcgcgt gcagaaaggc tcgttcggcg cgtgtctgat gaacgaagtc
    18901 gggctggttg ccgactgcct caacgccatg caggatgcgg tcaagattcc cgttaccgtc
    18961 aaacaccgca tcggtgtgga caggcagacc gaataccaaa ccgttgccga tttcgtcggc
    19021 acgctgcgcg acaaaaccgc ctgcaaaacc tttatcgtcc acgcccgcaa cgcttggctg
    19081 gacggtcttt cccccaaaga aaaccgcgac gttcccccgt tgaaatacga ttacgtttac
    19141 cgcctcaagc aggagtttcc cgggctggaa atcatcatca acggcggcat caccaccaac
    19201 gaagcaatcg caggacacct gcaacacgtt gacggcgtga tggtcgggcg cgaggcgtac
    19261 cacaacccga tggtgatgcg cgaatgggac aggctgtttt acggcgatac ccgcagcccg
    19321 attgaatacg ccgatttggt gcagcgtctc tacacataca gccaagccca aatccaagcc
    19381 ggacgcggca caatcttgcg tcacatcgtc cgccacagcc ttgggctgat gcacggtctg
    19441 aaaggcgcgc ggacttggcg gcgtatgctt tccgacgcaa cgctcttgaa agacaacgac
    19501 ggcagcctga ttctcgaagc gtggaaagag gtcgaacggg caaatatgcg cgaatagggc
    19561 ggggctgtat gtgtgaaatg ccgtctgaag gcttcagacg gcatttgtgc gtttgtcggg
    19621 cggtgtttag ggggcggtaa cggcgtgttt cggcactttg tccatatccc agtgtgccac
    19681 cgcccagtcg agcagttcgg cagggcggtc ggtttccggt gcttcgggca gcttgaggta
    19741 acggaacact tggcggagga gttgttcgcg gcggtttaaa tccaatgcgg gggcgagcgt
    19801 ctgtttcgac catttctgcc cttgtgcgtt ggtcagcagc ggcaggtggg catattgcgg
    19861 tgtcggaacg tccaaacact gctgcaaata gatttggcgc ggcgtggaaa cgagcaggtc
    19921 ttgtccgcgg acgatgtggg taacgccctg ttcggcatcg tcggcaacga cggcgagctg
    19981 gtatgcccag taaccgtctg cacgaagcag gacgaaatcg ccgatgtcgc gggcgaggtt
    20041 ttgggcgtaa ccgccgacga tgccgtctga aaaaccgata atgcggtcgg ggacgcggat
    20101 gcgccacgcc ggctgtttgc cttgcagtgc agggcgttgg ccggggtggc ggcaacgtcc
    20161 gttatagacg aacccgtctg cgccccgcct tgccccggcc tgccagtctt tgcggctgca
    20221 atggcaggga tagaccagtc cggcggtttt caggcggcat agggtttctt catacagggc
    20281 gtaacggcgg ctctgatagg cgacttctcc gtcccactcg aatccgaatg cctcaagcgt
    20341 gtgcaggata tggcttgccg cccccggcat ttcgcgcggc ggatcgaggt cttccatgcg
    20401 gatcagccat ttgccgccgt gcgcgcgcgc atcggcatag gaagcgacgg cggtcagcag
    20461 cgagccgatg tggagcagcc cggtcgggct gggggcaaaa cgtcctgtgt acatatctgg
    20521 tacagcccct ttatttaaga ctattaatca aagccattat ctcatcttta ttcagttcca
    20581 tcccgggctc ttcaagcaag gttaaatcat atagggcatt atattgctct tcggtagctg
    20641 aaccatccat aagagcaggc gagaaaaaat caaaggctct atctgcaatt ctctcattac
    20701 ttgcatttct actaaccagt ttcgtcaatt ctgtatattt tgaaaagttt atggaaaaat
    20761 aaaacagcga aaaagttttg gtttcgctgt ttttgattta attagcactg ataatcttca
    20821 aattcccacg aaaaaaaacg aagtaaataa gtcaatgact tttcccaagt ttcttttgaa
    20881 cattctttaa gaattttctc aatttccgat ttaataacag aatgattaaa ttcattcata
    20941 atcatcatac ccgcccccca tttaaccctt tgattttgga aacaattatg caaaatccat
    21001 ttaggagagc atatgcgaac agaaaatata tctgcagcat cactatcatc agttcctatg
    21061 tctaaatcaa ttcccacaca aaaattgtct ttgatttcgg gaacgaaatc ttcaaaggca
    21121 caatcgtaaa gattgatggc tttcaattct aggttaatca ttttatattc aatagtatgg
    21181 ggaggtaccg gatccttaaa aatcagatct gaataaattt cattgggtga aatgatttcg
    21241 attgcttttg ccatgattct atttcctttt gtgttagtgg gtaatgtcgt gcattaactt
    21301 cttgcccatt aatattttta gggtgaatcc ttgatatgcc gcactgtgtc cggtcaaacg
    21361 ggcgatgccg tctgaaagcc tttcagacgg catcgggaaa atgcctaagc caaaggcgcg
    21421 agcagttttt caaacgcttc ttcaaactgt ttcaaaccgt cttcctgcaa acgcgttgcc
    21481 aaggtttcga catcgatgcc gagcgcggcg gtttcggcga gctgcgcttg tgcttcttct
    21541 acgccttcgg tcagcgtggc tttggctgtg ccgtggtcga taaaggcttt gagcgtggca
    21601 tcgggaacgg tgttgacggt gtgcgcgccg atcaggctgt caacgtagag cgtgtcggga
    21661 taggccgggt ttttcacgcc ggtagatgcc cataaaagct gcacgcggtt tgcgcctttg
    21721 gtttccagcg cggcaaattc ggggctgccg aagtattgcg cccagtcttg gtaggcggct
    21781 ttggcaaggg cgatggcgat tttgcctttg aggtggtcgg gcagtgttgt gtccagcgcg
    21841 ccgtccacac gggagatgaa gaagctggcg acaacttgga tatgggcaac gctttgtccg
    21901 gctgctaagc gtttggcgat gccgcgcgcg taggcggcgt aggctttgag ggtttgggcg
    21961 cgtgagaaca gcagggtcag gttcacgctg atgccgtctg aaacgagggt ttcgagcgca
    22021 tcgatgcctg cgtcggtggc aggcacttta atcatcgcgt ttttgcaccc gatggcggcg
    22081 tagaggcggc gcgcttcttc aaccgtgcct tgcgcgtctt tggacaattc gggcgaaact
    22141 tcgaggctga cgaagccggt tttgccgccg gtggattcgt gttcggcaag gcaaacgtcg
    22201 caggcggcac gcacatcggc aaccgccatt gtttcgtagc gttgtttggg gctgaggttt
    22261 tgctgcttga gggcggcgat ttcatcggcg taaagcgcgt cgccggcgaa ggctttttgg
    22321 aagatggcgg gattggaagt tacgccgcac acgccctgtt tcaacatttg cgccaattcg
    22381 ccgctttgca ctagcgagcg ggaaaggttg tccagccaga tttgttgtcc taatgcttta
    22441 acgtccgata aaatggtcat ctctgattcc tttggatgga taggcggggt ttgagggctt
    22501 atgctacccc gattcggaaa ttttgggtag ttttattaca gcaaaggcgg atggcaatgg
    22561 cagaaaacgg aaaatatctc gactgggcac gcgaagtgtt gcacgccgaa gcggaaggct
    22621 tgcgcgaaat tgcagcggaa ttggacaaaa acttcgtcct tgcggcagac gcgttgttgc
    22681 actgcaaggg cagggtcgtt atcacgggca tgggcaagtc gggacatatc gggcgcaaaa
    22741 tggcggcaac tatggcctcg accggcacgc ctgcgttttt cgtccaccct gcggaagcgg
    22801 cacacggcga tttgggtatg attgtggaca acgacgtggt cgtcgcgatt tccaattccg
    22861 gcgaaagcga cgaaatcgcc gccatcatcc ccgcactcaa acgcaaagac atcacgcttg
    22921 tctgcatcac cgcccgcccc gattcaacca tggcgcgcca tgccgacatc cacatcacgg
    22981 cgtcggtttc caaagaagcc tgcccgctgg ggcttgcccc gaccaccagc accaccgccg
    23041 tcatggcttt gggcgatgcg ttggcggtcg tcctgctgcg cgcacgcgcg ttcacgcccg
    23101 acgatttcgc cttgagccat cctgccggca gcctcggcaa acgcctactt ttgcgcgttg
    23161 ccgacattat gcacaaaggc ggcggcctgc ctgccgtccg actcggcacg cccttgaaag
    23221 aagccatcgt cagcatgagt gaaaaagggc tgggcatgtt ggcggtaacg gacgggcaag
    23281 gccgtctgaa aggcgtattc accgacggcg atttgcgccg cctgtttcaa gaatgcgaca
    23341 attttaccgg tctttcgata gacgaagtca tgcatacgca tcctaaaacc atctccgccg
    23401 aacgtctcgc caccgaagcc ctgaaagtca tgcaggcaaa ccatgtgaac gggcttctgg
    23461 ttaccgatgc agatggcgtg ctgatcggcg cgctgaatat gcacgacctg ctggcggcac
    23521 gga
//
LOCUS       DAWGRZ010000012        23348 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000012 DAWGRZ010000000
VERSION     DAWGRZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23348
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.012"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            55..6585
                     /locus_tag="WHT44_000336"
     CDS             55..6585
                     /locus_tag="WHT44_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HEZ2532573.1"
                     /translation="MFASSGNVAIDANGRLVNSGTMAAANAKDTDNTAEHKVNIRSQG
                     VENSGTAVSQQGTQIHSQSIQNTGTLLSSGEILIHNSGSLKNETSGTIEAARLAIDTD
                     TLNNQGKLSQTGSQKLHIDAQGKMDNRGRMGLQDTAPTASNGSSNQTGNSYNASFHSS
                     TTTPTTATGTGTATVSISNITAPTFADGTIRTHGALDNSGSIIANGQTDVSAQQGLNN
                     AGQIDIHQLNAKGSAFDNHNGTIISDAVHIQAGSLNNQNGNITTRQQLEIETDQLDNA
                     HGKLLSAEIADLAVSGSLNNQNGEIATNQQLIIHDGQQSTAVIDNTNGTIQSGRDVAI
                     QAKSLSNNGTLAADNKLDIALQDDFYVERNIVAGNELSLSTRGSLKNSHTLQAGKRIR
                     IKANNLDNAAQGNIQSGGTTDIGTQHNLTNRGLIDGQQTKIQAGQMNNIGTGRIYGDN
                     IAIAATRLDNQDENGTGAAIAARENLNLGIGQLNNRENSLIYSGNDMAVGGALDTNGQ
                     ATGKAQRIHNAGATIEAAGKMRLGVEKLHNTNEHLKTQLVETGREHIVDYEAFGRHEL
                     LREGTQHELGWSVYNDESDHLRTPDGAAHENWHKYDYEKVTQKTQVTQTAPAKIISGN
                     DLTIDGKEVFNTDSQIIAGGNLIVQTEKDGLHNEQTFGEKKVFSENGKLHSYWREKHK
                     GRDSTGHSEQNYTLPEEITRNISLGSFAYESHRKALSHHAPSQGTELPQSNGISLPYT
                     SNSFTPLPSSSLYIINPVNKGYLVETDPRFANYRQWLGSDYMLDSLKLDPNNLHKRLG
                     DGYYEQRLINEQIAELTGHRRLDGYQNDEEQFKALMDNGATAARSMNLSVGIALSAEQ
                     VAQLTSDIVWLVQKEVKLPDGGTQTVLVPQVYVRVKNGDIDGKGALLSGSNTQINVSG
                     SLKNSGTIAGRNALIINTDTLDNIGGRIHAQKSAVTATQDINNIGGMLSAEQTLLLNA
                     GNNINSQSTTASSQNTQGSSTYLDRMAGIYITGKEKGVLAAQAGKDINIIAGQISNQS
                     EQGQTRLQAGRDINLDTVQTSKHQATHFDADNHVIRGSTNEVGSSIQTKGDVTLLSGN
                     NLNAKAAEVSSANGTLAVSAKNDINISAGINTTHVDDASKHTGRSGGGNKLVITDKAQ
                     SHHETAQSSTFEGKQVVLQAGNDANILGSNVISDNGTQIQAGNHVRIGTTQTQSQSET
                     YHQTQKSGLMSAGIGFTIGSKTNTQENQSQSNEHTGSTVGSLKGDTTIVAGKHYEQIG
                     STVSSPEGNNTIYAQSIDIQAAHNKLNSNTTQTYEQKGLTVAFSSPVTDLAQQAIAVA
                     QSSKQVGQSKNDRVNAMAAANAGWQAYQTGKSAQNLANGTTNAKQVSISITYGEQQNR
                     QTTQVQANQAQASQIQAGGKTTLIATGAAEQSNINIAGSDVAGKAGTILIADNDITLQ
                     SAEQSNTERGQNKSAGWNAGAAVSFGQGGWSLGVTAGGNVGKGYGNGDSITHRHSHIG
                     DKGSQTLIQSGGDTTIKGAQVRGKGVQVNAKNLSIQSVQDRETYQSKQQNASAQVTVG
                     YGFSAGGDYSQSKIRADHVSVTEQSGIYAGEDGYQIKVGNHTDLKGGIITSTQSAEDK
                     GKNRFQTATLTHSDIKNHSQYKGESFGLGASASISGKTLGQGAQNKPQNKHLTSVADK
                     NSASSSVGYGSDSDSQSSITKSGINTRNIQITDEAAQIRLTGKTAAQTKADIDTNVTT
                     DTAERHSGSLKNTFNKEAVQSELDLQRTVSQDFSKNVQQANTEINQHLDKLKADKEAA
                     ETAAAEALANGDMETAKRKAHEAQDAAAKADNWQQGKVILNMLASGLAAPTQSGAGIA
                     AATASPAVSYAIGQHFKDLAGQNANGKLTASQETAHVLAHAVLGAAVAAVGDNNALAG
                     ALSAGGSEAAAPYISKWLYGKEKGSDLTAEEKETVTAITNVLGTATGAAVGNSATDAA
                     QGSLNAQSAVENNDTVEQVKFALRHPRIAIAIGSVHKDPGSTLEPNISTIASTFQLNL
                     FPNSEFGGEGGVGNAFRHVLWQATITREFGKDIAVKVGNSHESGEKINYSIRRNLSLD
                     KADEMIDQLNNEIGREIALNTNRLNTKELVGLILETYKNNGFYQAERNSNGNYDVVRK
                     RLSEKDYQNTSNILIHLDNTGAGFKIQQRRKQIRAQISARQWRR"
     gene            6589..7005
                     /locus_tag="WHT44_000337"
     CDS             6589..7005
                     /locus_tag="WHT44_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532574.1"
                     /translation="MNKNILYIFSLLITIVIFFIFEKNVIRKISFNYNKKEFLISDIT
                     NFNWDYVKLYIINSDFQKIVFYHKSKIVFEELIELDKEGNVLPQYLFDSDLKNVEYYE
                     CDYKNGKMQLLKKEKSHFFDGYFYYYKPINCRPKLL"
     gene            7074..8087
                     /locus_tag="WHT44_000338"
     CDS             7074..8087
                     /locus_tag="WHT44_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HEZ2532575.1"
                     /translation="MAWSMFATTQADRAVRSATAPKEMWFHKKIIDEKTGKVSFDTRQ
                     IWSLNDLSKEELASIQDTNGKVITVSNPGIFNNREDSLSNAAKQNRNSTNGSGVIAVM
                     NPPTGKYKSDSNNKIKDFLWLGSSLVSELMYVGYDQLNNKVFQGYLPKTNSEKLNQDI
                     YREVQKMGNGWSVDTSNHSRGGITASVSLKDWVNNQKQNGIAPIRKARFYGTATNVQN
                     DYADVLQKNGYTYTGADGKTYNSGSYSIVHDKDFVGNKWIPFLLGTNDTTQGTCKGLC
                     YSHSSYFAEVPKAGTKEFDDYVKIWGEVEYDAQGKPINKSKPILVEPNKTKDNEKYEK
                     EAF"
     gene            8059..8367
                     /locus_tag="WHT44_000339"
     CDS             8059..8367
                     /locus_tag="WHT44_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532576.1"
                     /translation="MKNMKKKLSKYSLFLSSVFCLTACATTLLGTVVVGTALYGTSSN
                     WTTTDKEHQEIMNCLDKALVKLNIYFENDEEKYKNKTVMDIYYQCIKNPNYVVTHNNL
                     "
     gene            8379..8693
                     /locus_tag="WHT44_000340"
     CDS             8379..8693
                     /locus_tag="WHT44_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532577.1"
                     /translation="MKKTLSNLVLISFCSTMLTACPFWGSCADWNCSKLNPRWEKAVD
                     TCETENLTFVLHELGNQLGYKSITEVSFSEIGQHISYQCIHHGGGYNIRKDKQYGHLF
                     VQ"
     gene            8859..9317
                     /locus_tag="WHT44_000341"
     CDS             8859..9317
                     /locus_tag="WHT44_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HEZ2532578.1"
                     /translation="MPITPPLNIISPKLYPNEQWNESEALGAITWLWYQSPTHRQVPI
                     VEMMTYILPVLKNGQFALFCKGTQPIGYISWAYFDEVAQAHYLESDRHLRDNSDWNCG
                     DNIWLIQWFAPLGHSHQMRSAVRQLFPSTTVRALYHKGSDKGLRILTFKT"
     gene            9372..11159
                     /locus_tag="WHT44_000342"
     CDS             9372..11159
                     /locus_tag="WHT44_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEZ2532579.1"
                     /translation="MKLPLSYLPNIRFLSWCCLLAGIIAPATLLASPNPAEIRMQQDI
                     QQRQREEQLRQTMQPESDVRLHQKNTGETVNQLMGDDSSQPCFAINEVVLEGEHHARF
                     QFALKRALRETGFQAGKCLHAGNINQIMSLAQNALIGRGYTTTRILAAPQDLNSGKLQ
                     LTLIPSYLRSIRIDRSNDDQTHAGRIAAFQNKFPTRSNDLLNLRDLEQGLENLKRLPT
                     AEADLQIVPVEGEPNQSDVVVQWRQRLLPYRVSVGMDNSGSEATGKYQGNITFSADNP
                     LGLSDMFYVNYGRSIGGTPDEESFDGHRKEGGSNNYAVHYSAPFGKWTWAFNHNGYRY
                     HQAVSGLSEVYDYNGKSYNTDFGFNRLLYRDAKRKTYLGVKLWMRETKSYIDDAELTV
                     QRRKTAGWLAELSHKEYIGRSTADFKLKYKRGTGMKDALRAPEEAFGEGTSRMKIWTA
                     SADVNTPFQIGKQLFAYDTSVHAQWNKTPLTSQDKLAIGGHHTVRGFDGEMSLSAERG
                     WYWRNDLSWQFKPGHQLYLGADVGHVSGQSAKWLSGQTLVGTAIGIRGQIKLGGNLHY
                     DIFTGRALKKPEFFQSRKWASGFQVGYTF"
     gene            complement(11484..12773)
                     /locus_tag="WHT44_000343"
     CDS             complement(11484..12773)
                     /locus_tag="WHT44_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEZ2532580.1"
                     /translation="MMKSILTVSGNRMRKPRITYLDVWANDERIGTLEKGAMYRFAYD
                     NPNSSLLGLHYQDRSKVYISNNMPHIFAQYFPEGFLDAHITSKYAFHDAPFEDNEMLR
                     LAILCRETLGRIHVRCNDPLFNEWIDGLEMKNPRILTERDLLGINARQVFQQYMAEIF
                     HHGRFVSVSGIQQKMSLDAIRRNTKQTASYIAKGFDASEYPCLAANEFLCMQTIKQAG
                     IAVAQTSLSEDSSVLLVRRFDVSEQGYFLGMEDFTSLRQYSVEDKYKGSYAAIAQIIR
                     QISGRPDEDLIHFFNQLAASCILKNGDAHLKNFSVLYHDEYDVRLAPVYDVLDTSIYR
                     VGTQGIFDAYDDTLALNLTNHGKKTYPSKNTLLDFAEKYCDLGREDASFMIDTIVQAK
                     EQVLVKYSDVLRENEWLAQKWHFIPDENEEGLPFTFR"
     gene            complement(12900..13406)
                     /locus_tag="WHT44_000344"
     CDS             complement(12900..13406)
                     /locus_tag="WHT44_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532581.1"
                     /translation="METTFALLNFKESLCYIYPPTEPVRYVSSIVALNVHSPNGTGDW
                     HSAKALSDRAYPEKFYIYGENQERNTNHLFGDNGIIDGTDRLNKMGYFPENIPVWLAD
                     HPRACVDYLYTAVLQTGSIGRVILDDWFPSDEDKQSVYDLLNQIEPHLNTQEWENLQL
                     WKRKNPIM"
     gene            complement(13767..15131)
                     /locus_tag="WHT44_000345"
     CDS             complement(13767..15131)
                     /locus_tag="WHT44_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ2532582.1"
                     /translation="MQHLTERMTQIAKLLNSSANNPDIDIPDFLTEIKDYSEFSVTDE
                     NGTYLHWDKFRRIHTEDTRMKWRAVKESRKKIQKPIDFPFEHQFWFCIPDSLQARLHL
                     IDKSCGSSIGTSSLGGFGRSEQNRFLLKSLIMEEAITSAQLEGAATTRKVAKDMLKSQ
                     RKPKTKDEIMIVNNYHLMKKAVELKNTPLSVEMILDLHRIATSNAIENKAEPGQFRQD
                     DEIFIADINGNSLYQPPPHGQVHTLMEEVCAFANNTYDGVENPFIHPVVQAIILHFLI
                     GYIHPFGDGNGRTARALFYWFMLKNGYWLFEYISISRLLKNAPAQYAKSYLYAETDDL
                     DLTYFIYYQCDIIKRAVADLEHYISDKQKHQQEFKAAIAQYTEKIGKLNQRQIGILQK
                     AVEESGKIFTAQEIANQYGISLNTARSDLSKLGEYRFLVPFKSGNALEYVAPQDLLER
                     LEKK"
     gene            15552..15914
                     /locus_tag="WHT44_000346"
     CDS             15552..15914
                     /locus_tag="WHT44_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ2532583.1"
                     /translation="MKAARRQLYYTFANRIAEQSRIICIKKLNLLNLQCIQIKVNKLH
                     QKIVASSLLLHMLKEGAAKNGVHIEEIKANATSQSCYSCGRLNKRIGVAATRTNMVCE
                     SFGRHYDTDENASSNIRK"
     gene            complement(17449..17886)
                     /locus_tag="WHT44_000347"
     CDS             complement(17449..17886)
                     /locus_tag="WHT44_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapD"
                     /protein_id="HEZ2532584.1"
                     /translation="MSRYLITFDMDTNCLKDNYHGNNYTNAYSDIKTILARHGFENIQ
                     GSVYLGREGISEAHGTIAIQELTARFDWFYSCISNIKFYRLESDLNAQFIADGVYQAK
                     QAFLQRVEQLRISLTEAGLSDEQINQVLEKQKFELESPNLKLN"
     gene            18821..19237
                     /locus_tag="WHT44_000348"
                     /pseudo
     CDS             18821..19237
                     /locus_tag="WHT44_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360042.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            19413..19649
                     /locus_tag="WHT44_000349"
                     /pseudo
     CDS             19413..19649
                     /locus_tag="WHT44_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086896509.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(19702..19890)
                     /locus_tag="WHT44_000350"
     CDS             complement(19702..19890)
                     /locus_tag="WHT44_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HEZ2532585.1"
                     /translation="MTVKYTIFFSTSHHSNQTKATHRHTHVGSTAGQTTIRSGGDTTL
                     KGAQLIGIGIQADPQLQP"
     gene            complement(20035..20985)
                     /locus_tag="WHT44_000351"
     CDS             complement(20035..20985)
                     /locus_tag="WHT44_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ2532586.1"
                     /translation="MYLGIDVSKLTIDCCLIVDGQNYQKKFQNNKGGFEQLINWLQSH
                     KVNDKLHCVCEATGTYYEALAEYLYSRYTITVENPRKIKGYAIAELQRSKTDTQDAKL
                     IAQYCQDRKHKLKAWKPPTKEQKQLQEIARYLDYLKQQRATEKAKQHEAPDYIKSHIQ
                     TTISNLTAQIQIVKKQLLQFYKDNPSYNNLRKRLKTITGIGEQATAVLLSTYKRHEFK
                     NARQFTAYLGLDPRKFQSGTSVNGKSRISKIGSSEIRKSLYMPALVAYRCNAFPEFVG
                     RLKNKGKHIKLILIAIMRKLAVIAFTILQNGQDFQVERYK"
     gene            complement(21611..22696)
                     /locus_tag="WHT44_000352"
     CDS             complement(21611..22696)
                     /locus_tag="WHT44_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HEZ2532587.1"
                     /translation="MIYLFTGNMGTGKTSRVVSMILNNEDGLFKMKLEDGTEVDRPLY
                     FCHIDGLDKRQFKAHELTEEQIMSAPLRDVIPEGAVLIVDEAHYTYPVRAAGRPVPPY
                     IQELTELRHHGHTVILMTQHPSQLDIFVRNLVSKHVHLERKAIGMKQYYWYKCVTSLD
                     NPAGVSGVEVASWKPPKEAFKYYKSASQHQKFKKKVPWAVWALIAIVGFVGWKSYGIF
                     KVYSKATDSRIEQEAQKESVVQTMTEQPASSEEMPLKNSDNLKPEDFVPTLPEKPESK
                     PIYNTVRQVKTFEQIAGCIDGGKSDCTCYSNQGTPLKEITKIMCKEYVKNGLPFNPYK
                     DEQQRTEQVEQSAKADKPQVLVMGGKP"
     gene            complement(22706..22996)
                     /locus_tag="WHT44_000353"
     CDS             complement(22706..22996)
                     /locus_tag="WHT44_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ2532588.1"
                     /translation="MPLLAGLIPLLGILLKMLIVRIILATGLTFVTYAGYLIALEKFK
                     DYTSNAINSMPSDILNLLLISGFGQGLGYLFGAFSFFIGMHAFKKLTFVFPG"
     gene            complement(22997..>23348)
                     /locus_tag="WHT44_000354"
     CDS             complement(22997..>23348)
                     /locus_tag="WHT44_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ2532589.1"
                     /translation="RPNGRHRKERKEGEDGGLLCDYFPEILACQEMGKPSDGMFHDIS
                     IPQVIDDKTWSSHNFLPSNGVCPQPKTFHVFGRQYQASYEPLCVFAEKIRFAVLLAFI
                     IMSAFVVFGSLKGK"
BASE COUNT         7062 a         4996 c         4963 g         6327 t
ORIGIN      
        1 aaaatcacct tgatcagtac ggccgagcaa gcaggcattc gtaatcaagg gcagttgttt
       61 gcttcttccg gtaatgtggc gattgatgca aatggccgtt tagtcaatag tggcacgatg
      121 gctgccgcca atgcgaaaga tacggataat acagcggaac acaaagtcaa tatccgcagt
      181 cagggcgttg aaaacagcgg tacggcggta tcgcaacaag gcactcaaat tcacagtcag
      241 tcgattcaaa acactggcac attattgtcc tcaggcgaaa tattgattca caattcgggc
      301 agcctgaaaa atgaaacatc aggcaccatt gaagccgctc gtttggctat tgataccgac
      361 acacttaata atcaaggcaa actctctcaa acaggttcac aaaaactcca tattgatgca
      421 caaggcaaaa tggataaccg tggccgcatg ggtttacaag ataccgcacc aaccgcgtca
      481 aatggttcaa gcaatcaaac cggcaatagt tacaatgcat ctttccattc atccactacc
      541 acaccaacaa cggcaacagg tacgggtact gcaaccgttt ctatatcaaa cataactgcg
      601 cctacctttg ctgatgggac aattcgcact catggtgcac tggataattc aggcagtatt
      661 attgccaatg gtcaaacaga tgttagtgcg caacaaggtt taaataatgc aggacaaata
      721 gacattcatc agttaaatgc aaaaggttcg gcgtttgaca atcacaatgg aacaattatc
      781 agtgatgcgg tccacattca agccggcagc ctgaataatc aaaatggcaa catcacaaca
      841 cgccaacagt tagagattga aaccgatcaa ctggataacg ctcatggcaa gttattatca
      901 gcagaaatag cggatttagc cgtttcaggc agcctgaaca atcaaaatgg cgaaatagcg
      961 accaatcaac aactgattat tcacgatggt cagcaatcta ccgctgtcat tgataatacg
     1021 aatggcacga tacaatcagg ccgtgatgtt gctattcagg caaaatcgtt atccaacaac
     1081 ggcacacttg ccgctgataa taaactggat attgcgttac aagatgattt ttatgtagaa
     1141 cgcaatatcg tggcgggcaa tgaattgtcg ctcagtacac gaggcagcct gaaaaattca
     1201 catactttgc aagcaggaaa acgcattcgg attaaagcaa ataaccttga taatgcagca
     1261 caaggcaaca ttcaatccgg cggtacgaca gacattggca cgcagcacaa tttaaccaat
     1321 agaggcttga ttgacggaca acaaaccaaa atccaagccg ggcaaatgaa taatatcggt
     1381 acaggtcgga tttatggcga caatatcgct attgcggcta cccgcttaga caatcaagat
     1441 gaaaacggta caggtgccgc cattgcggca cgtgaaaacc tgaatttagg catcggacaa
     1501 ttaaacaacc gtgaaaacag tctgatttac agcggtaacg atatggcggt tggcggcgca
     1561 ttagatacca atggccaagc cacaggcaaa gcccaaagga tacacaatgc cggcgcaacc
     1621 attgaagctg caggcaaaat gcgtttaggt gtagaaaagc tgcacaatac caatgagcat
     1681 ttgaaaacgc agttggtaga aacagggcgc gagcatattg ttgattacga agcatttgga
     1741 cgacacgaat tattgcgaga aggcacgcaa catgaattag gctggtctgt ctataacgat
     1801 gaatcagacc acttacgcac ccctgatgga gcggcgcatg aaaattggca taaatacgat
     1861 tatgaaaaag tcacccaaaa aacccaagtt acccaaactg cgccagccaa aatcatttca
     1921 ggtaatgatt taaccattga tggtaaagaa gtatttaata ccgatagcca aatcattgct
     1981 ggtggcaatc tcattgtaca aacagaaaaa gacggtttgc ataacgagca aacctttggc
     2041 gaaaagaaag tattcagtga aaatggcaaa ttacacagct attggcgtga gaaacataaa
     2101 ggacgagact caacgggaca tagcgaacaa aattacactt tgccggagga aatcacacgc
     2161 aacatttcac tgggttcatt tgcctatgaa tcgcatcgca aagcattaag ccatcatgcg
     2221 cccagccaag gcactgagtt gccgcaaagc aacggtattt cgctacccta tacgtccaat
     2281 tcttttaccc cattacccag cagcagctta tacattatca atcctgtcaa taaaggctat
     2341 cttgttgaaa ccgatccacg ctttgccaac taccgtcaat ggttgggtag tgactatatg
     2401 ctggacagcc tcaaactaga cccaaacaat ttacataaac gtttgggtga tggttattac
     2461 gagcaacgtt taatcaatga acaaatcgca gagctgacag ggcatcgtcg tttagacggt
     2521 tatcaaaacg acgaagaaca atttaaagcc ttaatggata atggcgcgac tgcggcacgt
     2581 tcgatgaatc tcagcgttgg cattgcatta agtgccgagc aagtagcgca actgaccagc
     2641 gatattgttt ggttggtaca aaaagaagtt aagcttcctg atggcggcac acaaaccgta
     2701 ttggtgccac aggtttatgt acgcgttaaa aatggcgaca tagacggtaa aggtgcattg
     2761 ttgtcaggca gcaatacaca aatcaatgtt tcaggcagcc tgaaaaactc aggcacgatt
     2821 gcagggcgca atgcgcttat tatcaatacc gatacgctag acaatatcgg tgggcgtatt
     2881 catgcgcaaa aatcagcggt tacggccaca caagacatca ataatattgg cggcatgctt
     2941 tctgccgaac agacattatt gctcaacgca ggcaacaaca tcaacagcca aagcaccacc
     3001 gccagcagtc aaaatacaca aggcagcagc acctacctag accgaatggc aggtatttat
     3061 atcacaggca aagaaaaagg tgttttagca gcgcaggcag gaaaagacat caacatcatt
     3121 gccggtcaaa tcagcaatca atcagagcaa gggcaaaccc ggctgcaagc agggcgcgac
     3181 attaacctag atacggtaca aaccagcaaa catcaagcaa cccattttga tgccgataac
     3241 catgttattc gcggttcaac gaacgaagtc ggcagcagca ttcaaacaaa aggcgatgtt
     3301 accctattgt cagggaataa cctcaatgcc aaagctgccg aagtcagcag cgcaaacggt
     3361 acactcgctg tgtctgccaa aaatgacatc aacatcagcg caggcatcaa cacgacccat
     3421 gttgatgatg cgtccaaaca cacaggcaga agcggtggtg gcaataaatt agtcattacc
     3481 gataaagccc aaagtcatca cgaaaccgcc caaagcagca cctttgaagg caagcaagtt
     3541 gtattgcagg caggaaacga tgccaacatc cttggcagca atgttatttc cgataatggc
     3601 acccagattc aagcaggcaa tcatgttcgc attggtacaa cccaaactca aagccaaagc
     3661 gaaacctatc atcaaaccca gaaatcagga ttgatgagtg caggtatcgg cttcactatt
     3721 ggcagcaaga caaacacaca agaaaaccaa tcccaaagca acgaacatac aggcagtacc
     3781 gtaggcagct tgaaaggcga taccaccatt gttgcaggca aacactacga acaaatcggc
     3841 agtaccgttt ccagcccgga aggcaacaat accatctatg cccaaagcat agacattcaa
     3901 gcggcacaca acaaattaaa cagtaatacc acccaaacct atgaacaaaa aggcctaacg
     3961 gtggcattca gttcgcccgt taccgatttg gcacaacaag cgattgccgt agcacaaagc
     4021 agcaaacaag tcggacaaag caaaaacgac cgcgttaatg ccatggcggc tgccaatgca
     4081 ggctggcaag cctatcaaac aggtaagagt gcacaaaact tagccaatgg tacaaccaat
     4141 gccaaacaag tcagcatctc cataacctac ggcgaacagc aaaaccgaca aaccacccaa
     4201 gttcaagcca atcaagccca agcgagtcaa attcaagcag gtggtaaaac cacattaatc
     4261 gccacaggcg cagcagaaca atccaatatc aacatcgcag gctcagatgt tgccggcaaa
     4321 gcaggcacaa tcctgattgc cgataacgac atcacactcc aatcagccga gcaaagcaat
     4381 accgaacgcg gccaaaacaa atcggcaggc tggaacgcag gtgctgccgt atcattcgga
     4441 caaggaggct ggtcattagg cgttaccgca ggcggcaatg tcggcaaagg ctacggcaat
     4501 ggcgacagca tcacccaccg ccatagccat atcggcgaca aaggcagcca aacccttatc
     4561 caaagcggtg gcgacactac catcaaaggc gcgcaagtac gcggcaaagg cgtacaagtc
     4621 aatgccaaaa acctaagtat tcaaagcgta caagatagag aaacctatca aagcaaacaa
     4681 caaaacgcca gtgcacaagt taccgtaggt tatggcttca gtgccggtgg cgattacagc
     4741 caaagcaaaa tccgagccga ccatgtttca gtaaccgagc aaagcggtat ttatgccgga
     4801 gaagacggct atcaaatcaa ggtcggaaac catacagacc tcaaaggcgg catcatcacc
     4861 agtacccaaa gcgcagaaga caagggtaaa aaccgctttc agacggccac cctcacccat
     4921 agcgacatca aaaaccacag ccaatacaaa ggcgaaagtt ttggattggg cgcaagtgcg
     4981 tccataagcg gcaaaacact gggacagggc gcacaaaata aacctcaaaa caaacacctg
     5041 acaagcgtag ccgataaaaa cagcgcaagt tcatcagtgg gttatggcag cgacagcgac
     5101 agtcaaagca gcatcacaaa aagcggcatc aacacccgca acattcaaat caccgacgaa
     5161 gccgcacaaa tccggctgac aggcaaaaca gcggcacaaa ccaaagccga tattgataca
     5221 aacgtaacca cagacaccgc cgaacgacat tcgggcagct tgaagaacac cttcaacaaa
     5281 gaagcggtgc aaagtgaact ggatttacaa agaaccgtca gccaagattt tagtaaaaat
     5341 gttcaacaag ccaataccga gattaaccaa catttagaca aactcaaagc agacaaagaa
     5401 gcagccgaaa cagcagcagc cgaggcatta gccaatggcg atatggaaac tgccaaacgc
     5461 aaagcccatg aagctcaaga tgcggcagca aaagcagata attggcaaca aggcaaagtc
     5521 attctcaaca tgttagcctc aggtttagct gcgccgaccc aaagcggagc gggcatcgct
     5581 gcggctaccg catcgccagc cgtatcgtat gcgattggac agcactttaa agatttagcc
     5641 ggtcaaaacg cgaatggtaa actaaccgcc agtcaagaaa ccgcacacgt tcttgcccac
     5701 gcggtattag gagcagcggt tgccgcagta ggagacaaca atgctctagc aggagcattg
     5761 agtgcgggcg ggtcggaagc ggctgcgcct tacatcagca aatggttata cggcaaagaa
     5821 aaaggaagcg acttaacggc ggaagagaaa gagactgtaa cagcgattac aaatgtattg
     5881 ggtacggcta cgggtgcggc agtcggcaac agcgcaacag atgcagcgca aggcagcctg
     5941 aatgcgcaaa gtgcggtgga gaataatgat actgtagagc aagtgaaatt tgctcttagg
     6001 caccctagaa ttgctattgc aattggatct gtacataaag atcctggctc tacattagag
     6061 cctaatattt caacaattgc ttcaactttt caattaaatt tatttcctaa tagtgaattt
     6121 ggtggtgaag gtggagttgg caatgcattc aggcacgttt tatggcaagc aaccatcaca
     6181 cgagaatttg gcaaagatat tgctgttaaa gtaggaaata gtcatgaaag tggggaaaaa
     6241 attaattatt ctataagacg taatctttca ttagataaag cagatgaaat gattgatcaa
     6301 ctaaataacg aaataggaag agaaatagca ttaaatacca ataggttaaa cacaaaagag
     6361 ttagttggat taattctgga aacttataaa aataatggtt tttatcaagc agaaagaaac
     6421 agtaatggaa attatgatgt tgtaagaaaa agattatctg aaaaagatta ccagaataca
     6481 agcaatatat tgattcactt agataatact ggtgccggat ttaaaattca gcagaggaga
     6541 aaacaaatca gagcacaaat ttcagccaga caatggagaa gataaaagat gaataaaaat
     6601 atactttata tattttcttt attaatcact attgttattt tctttatatt tgaaaagaat
     6661 gtaataagaa aaataagttt taattataat aaaaaagaat tcttaattag tgatataacg
     6721 aattttaatt gggattatgt aaaactgtat ataatcaatt cagatttcca aaaaattgtt
     6781 ttttatcata aaagtaagat agtttttgag gaattaatag aactagataa ggaagggaat
     6841 gttttacctc aatatctatt tgattcagat ttgaaaaatg ttgaatatta cgaatgtgat
     6901 tacaagaatg gcaagatgca acttttaaaa aaagaaaaat cacatttttt tgatggatat
     6961 ttttattatt ataaacctat aaattgtagg ccgaaacttt tgtaaaacta tccttcgtca
     7021 ttaccgccta ggattcagga ttaggactcg tttggggagc agggagttct gatatggctt
     7081 ggagtatgtt tgccacaacc caagccgata gagcggtaag gtctgcaact gcacctaaag
     7141 aaatgtggtt ccataagaag ataatagatg aaaaaacagg taaagtatcc tttgatacca
     7201 gacaaatttg gtcattgaat gatttaagca aggaagaact ggcaagcatt caagacacaa
     7261 atggcaaagt tattactgtg tctaatcctg gtattttcaa taatcgagaa gattcattaa
     7321 gcaacgcagc aaaacaaaat cgtaatagta caaacggtag tggtgttatt gcagtcatga
     7381 atcctccaac agggaaatat aaatctgatt ctaataacaa aataaaagat tttttatggc
     7441 tcggttcaag tcttgtttct gaactgatgt atgtcggtta cgaccaatta aataataaag
     7501 tgttccaagg ctatttaccc aaaaccaatt cagaaaaact gaatcaagat atttatcgag
     7561 aggttcaaaa aatgggtaac ggctggtcgg ttgataccag taatcacagt cgtgggggaa
     7621 ttacagcaag cgtttcctta aaagattggg taaacaatca aaaacaaaat ggcattgccc
     7681 caatcagaaa agcacgtttc tatggtacag ccacaaatgt gcagaatgat tacgccgatg
     7741 ttttacagaa aaacggctat acctatacgg gtgcagacgg caaaacttat aacagcggat
     7801 cctactcaat cgtgcatgat aaagattttg tggggaacaa atggatacct ttcttgctag
     7861 gaaccaatga caccacacaa ggtacatgta aggggttgtg ctattcgcat agcagttatt
     7921 ttgcggaggt gccaaaagca ggtacaaaag aatttgatga ctatgtaaaa atatggggtg
     7981 aagttgaata tgacgctcaa ggtaagccaa ttaacaaatc taaacccata ctggtagaac
     8041 caaacaaaac aaaagataat gaaaaatatg aaaaagaagc tttctaaata ttctctgttt
     8101 ctttcatctg ttttttgtct aacagcttgc gctactacct tattaggtac tgtcgtagtc
     8161 ggtacagcct tgtacggtac tagtagtaac tggacaacta ctgataagga acaccaagaa
     8221 attatgaatt gccttgataa ggcattagta aaactgaata tctattttga gaatgatgaa
     8281 gaaaagtaca aaaacaaaac agtaatggat atttactatc agtgcattaa aaatcctaat
     8341 tatgttgtaa cacacaataa cttataaagg agagagatat gaagaaaacc ctttctaatt
     8401 tagtgctaat atcattctgc tcaacaatgc taacagcttg ccctttttgg ggtagttgtg
     8461 cagactggaa ttgctccaaa cttaatccac gctgggagaa agccgttgac acatgtgaaa
     8521 ctgaaaatct cacctttgtt ttgcatgaat taggcaatca attaggatat aaatctatta
     8581 ccgaagtctc atttagtgaa attgggcaac atatttctta tcagtgtatt caccatggcg
     8641 gtggatacaa tattcgtaaa gataagcagt atggtcattt gtttgtgcaa taaccagcgt
     8701 agcctgtgca attaatgcga actgttacat ggtagggtgg gcaactcgtt gcccacgcag
     8761 ttcagtctca tgtgctaaaa ctatgtttta acgtgaaata tttcatttca ggctgaaacc
     8821 attttttgtt ttaaccaaca cgaaccctaa cttaaaatat gcccatcact ccacccttaa
     8881 acatcatctc tcctaaactc taccccaatg aacaatggaa cgaaagcgaa gcactcggtg
     8941 ccatcacttg gctatggtat cagtcgccta cgcatcgcca agtacctatt gtggagatga
     9001 tgacgtatat attgcctgtg ttaaaaaacg ggcagttcgc tttgttttgc aagggtaccc
     9061 aaccaatcgg ttatatctca tgggcttatt ttgatgaagt ggcgcaggcg cattatttag
     9121 aatctgaccg ccatttgcgt gacaacagcg attggaactg tggcgacaat atttggctga
     9181 ttcaatggtt tgcgccattg ggacacagtc atcaaatgcg ctcagctgtg cgccagttat
     9241 ttcctagtac gacagtacgc gccttgtatc ataaagggag cgataagggt ttgagaattt
     9301 taacttttaa aacttgatgc aatcgtgtat tgaatgccca ttgatgattt tattgatttt
     9361 gcgaccatgc catgaaactt cctttatcct atttgcctaa tattcgcttt ttgtcttggt
     9421 gctgcttatt ggcaggtatc attgctcctg ctactttgtt ggcctccccc aaccctgccg
     9481 aaatccgtat gcagcaagat attcagcaac gccaacgcga agagcagttg cgccaaacca
     9541 tgcagcctga aagcgatgtg cgtttgcatc aaaaaaacac gggggaaacg gttaatcagt
     9601 tgatgggcga tgacagcagc caaccgtgtt ttgccattaa cgaagtggtg ttggaaggcg
     9661 aacaccatgc tcggtttcag tttgccctaa aacgtgcctt gcgcgaaacg ggttttcagg
     9721 ctggcaagtg tctgcatgcg ggcaacatta atcaaatcat gtccttagca caaaatgctt
     9781 tgatcggcag gggatatacc acgacccgta tcttggctgc gccacaggat ttgaatagtg
     9841 gcaagcttca attaaccctg ataccgagct atctgcgctc catacgaatc gatcggtcta
     9901 acgatgatca aacccatgca ggacgtattg cagcattcca gaacaaattt cccacccgct
     9961 cgaacgatct gttgaatctg cgtgatttgg aacaaggact ggaaaatctc aaacgtctcc
    10021 cgactgcgga agccgatctc caaatcgttc ccgtagaggg agaaccaaac caaagtgatg
    10081 tcgtggtgca atggcggcaa cgtctgctgc cctaccgtgt gagtgtgggg atggataatt
    10141 cgggtagtga ggcgacagga aaataccaag gaaatatcac tttctctgcc gacaatcctt
    10201 tgggactgag tgatatgttc tatgtaaatt atggacgttc gattggcggt acgcccgatg
    10261 aggaaagttt tgacggccat cgcaaagaag gcggatcaaa caattacgcc gtacattatt
    10321 cagccccttt cggtaaatgg acatgggcat tcaatcacaa tggctaccgt taccatcagg
    10381 cagtttccgg attatcggaa gtctatgact ataatggaaa aagttacaat actgatttcg
    10441 gcttcaaccg cctgttgtat cgtgatgcca aacgcaaaac ctatctcggt gtaaaactgt
    10501 ggatgaggga aacaaaaagt tacattgatg atgccgaact gactgtacaa cggcgtaaaa
    10561 ctgcgggttg gttggcagaa ctttcccaca aagaatatat cggtcgcagt acggcagatt
    10621 ttaagttgaa atataaacgc ggcaccggca tgaaagatgc tctgcgcgcg cctgaagaag
    10681 cctttggcga aggcacgtca cgtatgaaaa tttggacggc atcggctgat gtaaatactc
    10741 cttttcaaat cggtaaacag ctatttgcct atgacacatc cgttcatgca caatggaaca
    10801 aaaccccgct aacatcgcaa gacaaactgg ctatcggcgg acaccacacc gtacgtggct
    10861 tcgacggtga aatgagtttg tctgccgagc ggggatggta ttggcgcaac gatttgagct
    10921 ggcaatttaa accaggccat cagctttatc ttggggctga tgtaggacat gtttcaggac
    10981 aatccgccaa atggttatcg ggccaaactc tagtcggcac agcaattggg atacgcgggc
    11041 agataaagct tggcggcaac ctgcattacg atatatttac cggccgcgca ttgaaaaagc
    11101 ccgaattttt ccaatcaagg aaatgggcaa gcggttttca ggtaggctat acgttttaaa
    11161 acggcatagt caaatcaacg gtaactatag atatagcttt tcataataaa acacctatca
    11221 tgattatggt aagagatggc tgtgttttga tattagattg acacaggaat ttaagaagga
    11281 atcatgaaat acatgattcc ttctcgtttg cctacattaa acatataggc cgccaactca
    11341 atcaatagag tgatttacca gttttccaat cttcccagta tatctcctac cgaatacgat
    11401 tcttctaatt tctcggtttc aaacaccata agatgaagtg attttcaggg tgaaagtcga
    11461 ccggccatct ctgacagcgg cagctaccgg aatgtaaacg gtagaccttc ttcattttca
    11521 tccgggataa aatgccactt ctgcgccaac cattcattct cacgcaatac atccgagtat
    11581 ttaacaagaa cctgttcttt agcttgaacg attgtatcta tcataaagga tgcatcttct
    11641 cttcccaaat cgcaatattt ctcagcaaaa tccaacaatg tattcttgga aggatatgtt
    11701 ttcttaccgt ggttagtcag gtttaatgcc agcgtatcgt cataagcatc aaaaattcct
    11761 tgtgttccaa ccctgtatat tgatgtatcc aatacatcat agacaggtgc aagacgaaca
    11821 tcgtattcgt catgatagag tactgaaaaa tttttgaggt gtgcatcgcc gtttttcaat
    11881 atgcaactgg cagcaagctg attaaagaaa tggattaaat cttcatctgg tctgccggat
    11941 atctgtcgga taatctgtgc aatagccgca taactgcctt tatatttatc ttctaccgaa
    12001 tactggcgca gactggtaaa gtcttccatc cctaaaaaat aaccctgttc actgacatca
    12061 aaccgacgta ccaataagac tgatgaatct tccgacaggc tggtctgtgc aacggcaatg
    12121 ccggcttgtt tgatggtctg catgcataaa aattcattgg cagccaagca aggatattcg
    12181 gatgcatcaa aacctttggc aatatatgag gcagtttgct tggtatttct gcggatggca
    12241 tctaaggaca tcttctgctg tatcccggat acactgacga aacggccgtg atggaagatt
    12301 tctgccatat attgctgaaa aacctgtcgg gcatttatgc ccagcaaatc ccgttcagtc
    12361 aatattcttg gatttttcat ctccaacccg tcaatccatt cattaaaaag cgggtcatta
    12421 cagcgcacat gtatccgacc caaagtctct ctgcacagaa ttgccaagcg cagcatctca
    12481 ttgtcttcaa aaggcgcatc atgaaaagca tatttgcttg tgatgtgtgc atccaaaaag
    12541 ccttccggaa aatactgtgc aaagatatgc ggcatattgt tgctgatata taccttgctt
    12601 ctgtcttgat aatgcaggcc cagcaacgaa gaattgggat tgtcgtatgc gaaccgatac
    12661 atggccccct tttccaaagt accgattctt tcatcgtttg cccaaacatc caaataggtg
    12721 attctgggtt tacgcatacg atttccggat acggtgagga tagacttcat catgaaaaat
    12781 tctttcaaac gggaacagcg gtacgtttag acacatctgc tgatttaatt gtacatggcg
    12841 tattgcggat gaaattcagt aataccgctt ctcgtgcctt ttatctcgtt cggttagcat
    12901 tacattattg ggtttttgcg tttccatagc tgtaaattct cccattcttg agtattcaag
    12961 tgcggttcga tttggttaag taagtcataa actgattgct tgtcttcatc gcttggaaac
    13021 caatcatcta aaatcacccg accgattgag ccagtttgta acactgctgt gtaaagataa
    13081 tccacgcaag cacgggggtg atctgcgagc caaactggga tgttttcagg gaaataaccc
    13141 attttgttca gtcgatccgt cccatcaata atgccattat caccaaataa atgattggta
    13201 tttcgctctt gattttcacc gtaaatataa aatttttcag ggtaagcccg atcactcaat
    13261 gcctttgcac tatgccagtc gcctgtaccg ttaggactat gcacgtttaa ggctacaata
    13321 ctgctaacat atcgaacagg ttccgttggg gggtatatgt aacataaaga ttccttaaaa
    13381 tttagcagag caaaagtggt ttccacgttt taggacatat tttacaggat agtcacccca
    13441 ataatgtaag gctgtgtttt agtaatctgt tgatttcaat tacttgcaag ggaaaagaca
    13501 attattttcc ggttaggaat aaacctatcc tgttgaatac cttaaagcca aacacgccta
    13561 tcaacatcat attaaaacac agccaataat taaaagtatc gaattttcta accctgcttg
    13621 cttggaaagc aaaattgcta caatcttggt taaatcagct acaaagatca tttgtagagc
    13681 aaaaagcaag cagtccgcag cttctttttg aaggtgcgga ctgtattgat tttgactcgg
    13741 ttaaagctgc attctgggct agcaaactat tttttttcta acctttccaa taaatcctga
    13801 ggagcaacat actctaaagc atttcctgat ttgaacggca ctaggaatct atattctccc
    13861 agtttactca aatcgctacg ggcagtattc agggagatgc cgtattggtt ggcaatttct
    13921 tgtgcagtaa agatttttcc gctttcttcc actgcttttt gcaggatacc aatttgccgt
    13981 tggttcaact ttcctatctt ttcagtatat tgggcaatcg ctgctttgaa ttcctgttgg
    14041 tgtttttgtt tgtcggaaat gtagtgctcc aaatcggcaa ccgcccgctt gataatatcg
    14101 cattggtaat agatgaaata ggttaaatct aaatcgtcag tttccgcata caaataggat
    14161 ttggcgtatt gggcaggagc gtttttcaga agacggctga tggatatgta ttcaaatagc
    14221 cagtagccgt ttttgagcat aaaccaatag aacaaagccc gcgctgtccg cccgttgcca
    14281 tcaccaaatg ggtggatgta gccgatgagg aaatgcaaga taatagcttg gacaaccgga
    14341 tggataaacg gattttccac gccgtcatag gtattattgg caaacgcaca cacctcttcc
    14401 atcagcgtat gaacctgtcc gtgcggcggt ggttgataca ggctgttacc attgatatcg
    14461 gcgataaaga tttcgtcatc ctgcctgaat tgtccgggct cggccttgtt ttcaatagcg
    14521 ttactggtag caatgcggtg caaatccaaa atcatttcaa cacttaacgg cgtatttttc
    14581 aattctaccg cttttttcat caagtgatag ttgttcacta tcatgatttc gtcttttgtt
    14641 ttgggtttac gctgcgattt gagcatatcc ttggccactt tacgcgtggt agccgcacct
    14701 tccagttggg cggatgtaat cgcttcttcc ataatcagag acttgagcaa gaatctgttt
    14761 tgctcgcttc tgccgaagcc acccaagcta gacgtgccga tagaactgcc gcagcttttg
    14821 tcaatcaaat gaagccgtgc ctgcaaagag tcgggaatgc agaaccaaaa ctgatgttca
    14881 aacgggaaat caattggttt ttggattttt ttgcggcttt ccttaacggc gcgccatttc
    14941 atccgcgtat cttccgtgtg aatccggcgg aatttgtccc aatgcaggta ggttccattt
    15001 tcatctgtca cggaaaattc tgaataatct ttgatttcag taagaaaatc gggaatgtct
    15061 atatcaggat tgtttgcgga agaattcagc agcttggcaa tttgcgtcat tctttcagtg
    15121 agatgctgca tatattcctg ttgcaacagg gtaaattccg gaggtctgga aattttcatg
    15181 ataaatacca aaatcgcgca attttttata attgagctat tctagtcaat ttttatagaa
    15241 atagaaagca ttaaaatacg aatctgaaag aaacgatcac cttcataaat agggaaagat
    15301 tgtgtctact atattaaaaa aggctgtgtt ttaatatggt gttgataggc gtatttggct
    15361 ttaaggtatt caacaggata ggtttattcc taaccggaaa ataattgtct tttcccttgc
    15421 aaataattga aatcaataga ttactaaaac acagcctaat gtaaaaacaa attggtacaa
    15481 atggcgaaga taacatttgg ctcaaacgta ccgagcggct acgcattgaa tggtaaaata
    15541 cctaccgcaa attgaaagct gcaagacgac agctctacta tacgttcgca aacagaattg
    15601 ccgaacaaag ccggataatc tgtatcaaaa aattgaatct gctcaaccta cagtgcatac
    15661 agattaaagt caacaaactc caccagaaga tagttgcctc atccctactg ctgcatatgc
    15721 tgaaagaagg tgcagctaaa aatggagttc atatcgaaga gattaaagcc aatgctacca
    15781 gccaatcatg ttactcttgt ggtcgcttga acaaacggat aggtgttgct gccacaagaa
    15841 ccaatatggt ttgtgagagt tttggtcggc attacgacac tgatgaaaat gcctcctcca
    15901 atattcgaaa atagggagct gaaaagctag gcacatagag tagcagttga ccgtaaaatc
    15961 ataaacgctt cggaataaaa agatgtagtg atcgcatcct tgcattcctt tttatgtttt
    16021 gttgctttag cagttttaca gtttagcaac tgtacatttt ggcattttta acttttcata
    16081 tacgaaccaa catttttaaa tactgcaaat attttgtaca aaatatattc attatgcctt
    16141 tttgatttct catcattaat atctcgttta tattaatatt gcaaataatt taaacaaatt
    16201 ttaggaaagg aaactaattg cggtaatatc taaaatccaa agaactgctt ctttcttttt
    16261 taccttcttg ctggttttat gcttcaacag ttgtacagtt ttactccatg caaaccaaac
    16321 aacaaaaaaa gaccgacgaa gaagtcttct gcttagaatt taaagaatac tggagtaggg
    16381 gactcccgtt ttccgcgaca tcatttccaa atagacacgg ggtacgctca gcatttcatc
    16441 tgaaccatat ccatcttttt ccccagtttt aaccttgtga aacatccagt cctttttcaa
    16501 atcttcagac agaaaagaaa tactttcctg tttgattgac ttgaagagaa ccatcctacc
    16561 atcactcttg taagaaaagg cagtatctaa tacctatctt ctagagtcga tatactccct
    16621 tgacgaaaaa aatgatgata ttacggatac caaaactaag gtcgtatccg ccccccctac
    16681 tctccctaag caaagagatg aaacagcgta tcgactccct gccggttgaa ttttccgaaa
    16741 aaacgcgacg taaccagcat caacatatat aagaacagca caaatagcat caatacatca
    16801 ggcaacgaaa atgcagaata atgcacttaa tggtgtttgg atatctgttg ttttgtgctg
    16861 ttagtaattc ttctttctgt gtttacagtt tagcagttgt acagttttat agtaatgttt
    16921 aaacaatgac tgatttattt taaatgcaga tattgtagag gataaaaatg gccaaagtcc
    16981 tttcagtaac atttttgatt ttttagcgag ccttctcatt tccccagcga gatcggcaat
    17041 ggcagcggta ctttggccgc cgatatgctt aagttcagta accttagtgc gtaaatccag
    17101 taaccttaag ttacgtaaac cattagctgg cagggtgtga gttcgatttg agcaagcatt
    17161 attttgtctc ctgttgtacg gtttcagacg gcatcggttt gccacgtttg taccaagtgg
    17221 cgcgggaaat gccgagactc tcccacggtt tatcgcagct gcaattttaa actcctaaaa
    17281 taagtttgcc ttttttgtac taattactgc ttggatagtt tagattagac ttaagtaaat
    17341 ttcgagtgag ttgtttcaca ccctacccga agctctaaaa cgcacagaaa tcgccgcagc
    17401 gcgccaaaaa ctgacagagc tgcggcggat gttggtgagt aaaggaggtt aatttaattt
    17461 caggttagga ctttccaatt caaatttctg tttttccaga acctgattga tttgctcatc
    17521 agacaatcca gcttctgtta gggatatacg aagttgttca acacgttgaa ggaaagcctg
    17581 tttggcttga tacacaccat cagcgataaa ttgtgcgttc aaatcacttt caaggcggta
    17641 aaacttaatg tttgaaatac aggagtaaaa ccaatcaaac cgagcggtca gttcctgaat
    17701 ggctattgtt ccgtgtgctt cactgatgcc ttcacggcct agataaacac tgccctgaat
    17761 gttctcaaat ccatgtctag ccaagatggt tttaatatcg gagtaggcat tggtatagtt
    17821 atttccgtgg taattgtctt tcaggcagtt ggtatccata tcaaaggtaa tcaggtaacg
    17881 gctcattatt tttctcctgt tgcgtttcag acagcatcgg tttcccgcga tagtaccaag
    17941 tgcggcggct gataacgagc ttttcccacg gtttatcgcg gctgatagtg ttttcggcga
    18001 ggtagtcggc acgtgtttga gaccaccagc gagtgattgt gctttcagct acaataattt
    18061 tgctgcttgc cctatgttta aaaatctatc catattggat agtttagatt agacttaagt
    18121 agatttcaag tgagctgttt aacccttagc tagcaagggt tttggtggcg taaggttact
    18181 gaacttaagc atatcggcgg ccaaagtacc gctgccattg ccgatctcgc cggggaaatg
    18241 agaaggctcg ctaaaaaatc aaaaatgtta ctgaaaggac tttggccatt tttatcctct
    18301 acaatatctg catttaaaat aaatcagtca ttgtttaaac attactgtaa aactgtacaa
    18361 ctgctaaact gtaaacacag aaagaagaat tactaacagc acaaaacaac agatatccaa
    18421 acaccattaa gtgcattatt ctgcattttc gttgcctgat gtattgatgc tatttgtgct
    18481 gttcttatat atgttgatgc tggttacgtc gcgttttttc ggaaaattca accggcaggg
    18541 agccgatacg ctgtttcatc tctttgctta gggagagtag gggggcggat acgaccttag
    18601 ttttggtatc cgtaatatca tcattttttt cgtcaaggga atatacacgg aggatgattc
    18661 aaaaccggca ggcaaaaaaa gaccgcttct gaactgcatc ccaaaagttg gacaccttac
    18721 cgactttagg gggtgcagtt ttttatggca aaatattcag atgaattccg acttgccgtc
    18781 gttcaatact atttggcagg gaacagcaga ccatctttct atttccgatt cattggtacg
    18841 cagatgggtg acaaaataca gattacacgg agagagtggc atcaaacgta gaaagcatac
    18901 gacaaaatat tcggtcgaat acaaacttga ggcaatccgc ctggtggcgg ggtagggaat
    18961 gtcccaaaaa gctgccgcag accaactgaa tttgcccgac tgctccatct tgctgcaatg
    19021 gttgcgcctc taccatttga atggtattaa cggtttaaag cccaaaccct aaacccaaag
    19081 gaagaaagcc cgtgaaaaaa cagcatccgc cggaaacgaa aaagccgact atctgaaaac
    19141 caaggaagaa ctgcttgcgg aattggcttg ccttaaagcg gaaatggctg ccctaaaaaa
    19201 gctcgatgcc ttaatctatg ggaaagaagt gcggtagaaa gaacgcaact cgtcgcaggg
    19261 ttaaggcaat gccatccgtt gaactgctgt tggtgattgt cggactgcca cgcggcacct
    19321 tctattacca attggttgtc caatcggcag aagacaaata tgccgatttg aaacggcata
    19381 tccatgatat ttatcaaaag cagttgaaag acaacggtct ggttcagagt atgtcccgca
    19441 agggaaactg cttggacaat gcggcaatgg aaagtttctt cggaacgttg aaatcggaat
    19501 gtttccatac gtgcaaatat gattccgtta ccgaattgga agctgtactg cacgaatata
    19561 tccgttacta caacaacgat agaatcaagt tgaaattaaa aggactgagc cctgttcagt
    19621 acagaattca gtccctgaaa gccgcttgat taaactgtcc aacttttggg ggtcagttaa
    19681 tatcggttcc accaatagtc gtcagggttg tagttggggg tctgcctgta tgcctatgcc
    19741 gataagctga gcacctttga gggtggtatc cccgccgctt cggatggtgg tttgtccggc
    19801 cgtactgccg acatgggtgt ggcggtgggt tgcttttgtc tgattgctgt ggtggcttgt
    19861 tgaaaaaaag atagtgtatt tcacggtcaa ataactttac atttcttgaa gttgcaagaa
    19921 tgcgcacccc gactttttcg ggatgtttgt ttcgttttga atggatttga tgttttaatt
    19981 tatactttat tttcaataaa ttgaaaatca ccggcgaaag cccagtttaa ttttttattt
    20041 atatctttcc acttggaaat cttggccgtt ttgcaaaatc gtaaacgcta ttaccgccag
    20101 tttccgcatg atggcaatta atatcaattt tatatgcttc cctttatttt tcagacgccc
    20161 tacaaattca gggaaggcat tacaacgata tgcaacaagt gcaggcatat aaaggctttt
    20221 ccttatttcc gaacttccta tttttgatat tctgcttttt ccgttcacgc ttgttcctga
    20281 ttgaaatttt ctagggtcta ggcctagata agccgtgaac tgtcttgcat ttttaaattc
    20341 atgtctttta taggttgata gcaatactgc tgtcgcttgc tcgcctatgc ctgttattgt
    20401 tttcagcctt ttgcgtagat tgttataact tggattgtct ttgtagaact ggagtaattg
    20461 ctttttgact atctgtattt gtgctgtcag gtttgaaata gttgtttgaa tatgggattt
    20521 gatatagtcg ggtgcttcgt gttgtttagc tttttctgtt gcgcgttgct gtttcagata
    20581 gtctaaatat cgggcgattt cctgtaattg cttctgttct tttgtcggcg gtttccatgc
    20641 ttttaatttg tgctttcggt cttggcaata ttgggctatc aactttgcgt cttgtgtatc
    20701 tgtttttgat cgttgtagtt ctgctatcgc atatcctttt atctttcgtg gattctctac
    20761 ggtaattgta tatcttgaat aaagatattc ggctaatgct tcgtaatatg tgcctgttgc
    20821 ttcgcacacg caatggagct tatcgtttac tttatgactt tgtagccaat ttattaattg
    20881 ttcaaatcct cctttgttgt tctgaaactt cttttgataa ttttgaccgt ctacaatcaa
    20941 acagcaatct attgtgagct ttgaaacgtc tattcctaag tacatggttt aaccttatta
    21001 attcgggctt tttgccctag atagtgttca aacttaagat gtacgaaagc ccacgcttct
    21061 atctttctta caagctgtac gctttggccg tactttcgaa gtcgtgggct ttacttggtg
    21121 tttcgtcaaa cgccaagccc tcaatgggct gatttactca ttcagggctt gaagcttatc
    21181 gcctttgccg tatctgattt tattggggtc gcaggttttg gtaaaggttt ctgttgcgac
    21241 ccgaatgtct gatttttttt gggcgtatct cagtctggaa tcactccgtt cgggggtttc
    21301 cggtattgaa aaacagttca taaaaaagga aaagggggta ttcataaaga ttgggtaaaa
    21361 agcgcgccca atctttacaa agcttccccc ttttcctttt ttctgcccta ttttcctgca
    21421 cctacaaccc ccgaacgaag cgattccaga ctgagacact ttaaaaaaaa cagccattct
    21481 agcaattaac ccccttcact cagctcaagc catcctgagg ggtagggatt gaactttctg
    21541 ctttacgact ccgccgccaa ttccttcaaa cggttttccg cgctcttcag ttgtcgtaca
    21601 tgagattttg ctacggcttt ccgcccatta cgagaacttg cggcttgtcc gctttcgcgg
    21661 actgttccac ctgttccgtc ctttgctgtt cgtccttata aggattgaaa ggcaacccgt
    21721 ttttcacata ttctttacac attatctttg ttatttcttt caagggtgtt ccttgatttg
    21781 aatagcatgt gcaatctgat tttccgccgt ctatacatcc ggcgatttgc tcaaaggttt
    21841 ttacttgtcg gactgtgtta taaataggct tgctttcggg cttttcgggt aaagtcggca
    21901 caaagtcttc aggtttcaaa ttgtctgaat tttttaaagg catttcctct gatgatgcag
    21961 gctgctccgt catcgtctgc acaacgcttt ctttttgcgc ttcctgctca atccggctgt
    22021 ctgtggcttt gctgtaaact ttaaaaatgc cgtaactttt ccagcctaca aaccctacaa
    22081 tcgcaatcaa cgcccaaacc gcccaaggta cttttttctt gaacttttgg tgctggcttg
    22141 ctgatttata gtatttaaag gcttctttcg gcggtttcca acttgcgact tctacgccgc
    22201 ttacgcctgc gggattgtcc aacgaggtta cgcatttata ccaataatac tgtttcattc
    22261 cgattgcctt gcgttcaagg tgtacatgct ttgaaacaag gttgcggacg aatatatcaa
    22321 gttggctcgg gtgctgcgtc atcaaaataa cggtatgccc gtgatggcgg agttctgtca
    22381 gttcctgaat ataaggcgga acgggacggc ctgccgcgcg taccggataa gtgtagtgcg
    22441 cttcgtcaac aatcagcact gcgccttccg gtatgacatc acgaagcggg gcggacatga
    22501 tttgctcttc cgtcagttcg tgggctttaa actgccgttt atccaatccg tcgatatggc
    22561 agaaataaag cggtctgtct acctctgtgc cgtcttccaa tttcattttg aacaatccgt
    22621 cttcgttgtt caaaatcata gagacgacgc gggaggtttt gcctgtcccc atgtttcctg
    22681 taaacagata aatcatgctt ctacctcatc ctggaaagac aaacgtcagt tttttgaatg
    22741 cgtgcatacc aatgaagaac gagaatgcgc cgaacaggta gcccaacccc tgaccgaatc
    22801 ccgaaattaa aagaaggttc agtatgtcgg aaggcatgga attgatcgca tttgacgtgt
    22861 agtctttgaa cttttccagc gcgatgagat acccggcata ggttacaaat gtcagacctg
    22921 ttgcaaggat tattctgaca atcagcattt tcagaagtat gcctaaaagt ggaatcagac
    22981 cggcaagtaa tggcatttat ttccccttca acgaaccgaa aacgacaaaa gccgacataa
    23041 tgataaaggc gagcagtacg gcaaaacgga ttttttcggc aaacacgcat aacggctcat
    23101 agcttgcctg atattgccta ccgaaaacat gaaaggtttt cggctgcgga catacgccgt
    23161 tagacggtaa aaagttatgt gaagaccatg ttttatcgtc tataacctgc ggtatgctta
    23221 tatcgtgaaa catgccgtct gaaggtttgc ccatctcctg acaggctagg atttccggaa
    23281 aataatcgca caaaagcccg ccgtcttcgc cttctttcct ttctttgcga tgcctaccgt
    23341 ttgggcgg
//
LOCUS       DAWGRZ010000013        21295 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000013 DAWGRZ010000000
VERSION     DAWGRZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21295
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.013"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(59..1945)
                     /gene="uvrC"
                     /locus_tag="WHT44_000355"
     CDS             complement(59..1945)
                     /gene="uvrC"
                     /locus_tag="WHT44_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ2532590.1"
                     /translation="MPSETVFQVIDMNKETRFPEHFDIPLFLKNLPNLPGVYRFFNES
                     GNVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSESEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVREAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGLVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPVPDALKEALEGEHGKQMQFVTKTIGERKVRLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(2009..4171)
                     /locus_tag="WHT44_000356"
     CDS             complement(2009..4171)
                     /locus_tag="WHT44_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ2532591.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLFTINVPFLIG
                     MAGMMIGRHDWMIPPLWQFALASVVQLWLAIPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAYGMAHVYFEVGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRNGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRATQLGSQTQLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMG
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGSPQVAAVYCVPDSGFDEDALYRI
                     AAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQTVVGAGITAEVEGVGLVKAGKAEF
                     AELALPKFLDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALLVSQATLKNIKQNLFFAFFYNILG
                     IPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            4310..5260
                     /gene="trxB"
                     /locus_tag="WHT44_000357"
     CDS             4310..5260
                     /gene="trxB"
                     /locus_tag="WHT44_000357"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ2532592.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            5416..5784
                     /gene="rpsF"
                     /locus_tag="WHT44_000358"
     CDS             5416..5784
                     /gene="rpsF"
                     /locus_tag="WHT44_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ2532593.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            5785..6087
                     /gene="priB"
                     /locus_tag="WHT44_000359"
     CDS             5785..6087
                     /gene="priB"
                     /locus_tag="WHT44_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ2532594.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            6094..6324
                     /locus_tag="WHT44_000360"
     CDS             6094..6324
                     /locus_tag="WHT44_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ2532595.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            6341..6793
                     /locus_tag="WHT44_000361"
     CDS             6341..6793
                     /locus_tag="WHT44_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ2532596.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            complement(6986..7792)
                     /locus_tag="WHT44_000362"
     CDS             complement(6986..7792)
                     /locus_tag="WHT44_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ2532597.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFTMMPGMIFGVFTGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(7793..8539)
                     /gene="pssA"
                     /locus_tag="WHT44_000363"
     CDS             complement(7793..8539)
                     /gene="pssA"
                     /locus_tag="WHT44_000363"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ2532598.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            complement(8613..9017)
                     /locus_tag="WHT44_000364"
     CDS             complement(8613..9017)
                     /locus_tag="WHT44_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532599.1"
                     /translation="MANRFQQRKSAYRRLSGKPLFSDGIAANLCGGRKTLSYNLSRFK
                     ISIRSEMQKISFNLLKPANSPKIGKRRPGLNGAPHIPMPSPRYLPASPEMPGNNIHPA
                     KIRNNTPGGRQSQTAPQSIRHQKNKPPPRASS"
     gene            9123..9605
                     /locus_tag="WHT44_000365"
     CDS             9123..9605
                     /locus_tag="WHT44_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEZ2532600.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFSELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGALR
                     VDNAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(9657..10868)
                     /locus_tag="WHT44_000366"
     CDS             complement(9657..10868)
                     /locus_tag="WHT44_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ2532601.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            11091..13529
                     /locus_tag="WHT44_000367"
     CDS             11091..13529
                     /locus_tag="WHT44_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ2532602.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDEPEPLEKLPLIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSSTD
                     LNGSRKILAHKDHL"
     gene            13737..15047
                     /locus_tag="WHT44_000368"
     CDS             13737..15047
                     /locus_tag="WHT44_000368"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ2532603.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADPSRLQAPTSLAVESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            15143..15757
                     /gene="clpP"
                     /locus_tag="WHT44_000369"
     CDS             15143..15757
                     /gene="clpP"
                     /locus_tag="WHT44_000369"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ2532604.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCDRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLRL"
     gene            15912..16148
                     /locus_tag="WHT44_000370"
     CDS             15912..16148
                     /locus_tag="WHT44_000370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532605.1"
                     /translation="MPSESQMGQQFLQGRDTRILLNLPVRLLRKDKGRKRYPAFLIYT
                     CNSSPYLPIVFSYCASIPYRFKKMPFPMGWVLYQ"
     gene            complement(16200..17465)
                     /gene="ispG"
                     /locus_tag="WHT44_000371"
                     /gene_synonym="gcpE"
     CDS             complement(16200..17465)
                     /gene="ispG"
                     /locus_tag="WHT44_000371"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ2532606.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGLKRHQGKVIPIHSL"
     gene            complement(17481..18242)
                     /gene="pilW"
                     /locus_tag="WHT44_000372"
     CDS             complement(17481..18242)
                     /gene="pilW"
                     /locus_tag="WHT44_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ2532607.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(18245..19339)
                     /gene="rlmN"
                     /locus_tag="WHT44_000373"
     CDS             complement(18245..19339)
                     /gene="rlmN"
                     /locus_tag="WHT44_000373"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ2532608.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(19482..19907)
                     /gene="ndk"
                     /locus_tag="WHT44_000374"
     CDS             complement(19482..19907)
                     /gene="ndk"
                     /locus_tag="WHT44_000374"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ2532609.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(19966..21117)
                     /locus_tag="WHT44_000375"
     CDS             complement(19966..21117)
                     /locus_tag="WHT44_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ2532610.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
BASE COUNT         5101 a         5302 c         5788 g         5104 t
ORIGIN      
        1 ggtataccga cctgctgtat catatttttt acagacggat tttgtctttg acagcatgct
       61 aatgcagatg attgtaaatc gtttccgcca atgccttgct gatgccttcc actttttcca
      121 agtcctcgcg gctggcggca atcacgccgc gcagaccgcc gaagcgggtg agcagggctt
      181 ggcggcgttt gctgcctacg ccggggatgt cgcttaagga ggaggtaacg cgggctttgt
      241 cgcgtttttt gcggtgaccg gtaatggcga aacggtgcga ttcatcgcgt acggtttgca
      301 atagatgcaa ggccgggctg ttgggcggca ggcggaagac ttcgccggta aaaggcagta
      361 tgagctcctc cataccggct ttgcgctccg ggcctttggc aatgccgacc aaagggatgt
      421 gcagcccgag ttcttcccat accgatacgg ctacgccgat ttgccctttg ccgccgtcaa
      481 tcaacacggc atccggccat ttgacggtct cgccgttggc ttcggcctcc tgcattttgc
      541 cgtaacggcg cgtcaacact tcgcgcatgg cggcgtagtc gtcgccgggt ttggcggtcg
      601 tgatgttgta gcggcggtat tgcgaaggct ggatgttttg ctcatcgtac acaacgcagg
      661 acgcaatagt ggcttcgcct tgtgtgtggc tgatgtcgaa acattcaagg cggttgaggc
      721 cgtctgaatc catgccgagg atttttgcca gttcatcaat gcggtgttgc tggctgcttt
      781 gttgcaggcg gcgttgtgca atcgccattt gcgcgttttg ttccgccatt ttcaaccgga
      841 ctttgcgttc gcctatggtc ttggtgacaa attgcatctg cttgccgtgt tcgccttcca
      901 aagcctcttt tagcgcatcg ggaacgggaa agttgctgat gatgatgtcg ggtttgcttt
      961 tgcccagata gtgttgggcg acgaaggctt cggcgtaatc ttgtccgttt ggctcgggat
     1021 cgtttttggt gtcggggaaa aagcttttgt cgccgacgtg ccgtccgccg cggatgctga
     1081 cccagtgtac gcaaaccagg ccgtctgaaa ccgccagtgc aagcaaatcg atatcgttgg
     1141 gattgttcgg atttttactg tcgataaact gattactttg catgatgccg agcgcttgga
     1201 tttgatcgcg gtaacgtgcg gcttcttcga attgtagatt agcggcggcg gtttgcattt
     1261 tgtgttgcag ggtacgcgtc aattcgtcag ttttgccatt aaggaaagtc gcggcttcac
     1321 gcacgctgtc acgataatct tcttcactga tgtggcctac acaaggcgcg gtgcagcgtt
     1381 tgatttggta aagcagacaa ggacggtcgc gatgctcgaa tacactgtct tcgcaggtac
     1441 gcagcataaa gactttttgc aacacttgaa tgctgtcgcg cacggcgttg ctgttgggat
     1501 atgggccgaa atattgatta ggctttttca gcgtgccgcg gtaatacgcc atttgcggat
     1561 attgatggcc gctgagcatc aaataaggat agcttttgtc atcgcggaaa agaatattgt
     1621 atttgggcga cagggctttg atgaagttgt tttcgagaat cagggcttcg gattcggagc
     1681 gggtgatggt ggtttcgatg tggtgaacct gtttcaccat caatgcgatg cgcggggaat
     1741 ggtcgttttt ctggaaatag ccggacacgc gccgcttgag gttgacggct ttgccgacgt
     1801 ataagacgtt gccgctttcg ttgaaaaaac ggtatacgcc tggcaggttg ggcaggtttt
     1861 tgaggaaaag tgggatgtcg aagtgttccg gaaaacgggt ttctttattc atatcaatta
     1921 cttgaaaaac ggtttcagac ggcatattac catatcgctg gagacagcct tatccgttcg
     1981 gacaaggctg cagggcggtt acatgctgct aatcgatttt tacccgtttc aggcgcaagg
     2041 cattgctcaa cacggaaacc gagcttgccg ccattgccgc gccagcgatg acgggattta
     2101 aaaagccaag cgcggcgaga ggaatgccca aaatattgta gaagaaggcg aaaaacaggt
     2161 tttgcttgat gtttttcaaa gtggcttgcg acaccagcag agcatcggcg agttggttga
     2221 ccgaatgctg catcagcgtg gcggatgcgg tatgttcggc aacgtccgct ccgcctttca
     2281 tggcgaagct gacgttagcg gcggcaagcg cgggcgcgtc gttgatgccg tcgccgacca
     2341 tcgccacggt tttgccggcg gctttgagtt tttgcacttc ggcagcttta tcgcgcggac
     2401 tcatgttgcc gaaggcgtgt gcgatgccca gttgtttggc gacgtattcg accgtgcctt
     2461 ggttgtcgcc gctcataata tagacatcga tattgtgttt tttcagacgg cctatggctt
     2521 cggcggtatc ggctttcaac gcgtcggcaa gtgcgaatgc gccgatgggt ttgttatcga
     2581 ctgagaccgc aacaatgctt gcaatatccc aaacgccgtc taaaaacttc ggcaaggcca
     2641 gttcggcaaa ttcggctttg cctgctttca ccaaacccac gccttccact tcggcggtaa
     2701 tgcctgcgcc gacaacggtt tgtgcgtttt gtgcggcggg aatgtccaaa ccgcgcgctt
     2761 gggcggcgga gacgatggca cgggcgagcg gatgggcggc gttttgttcg acggcggcgg
     2821 cgatgcggta caaagcgtct tcgtcaaagc cgctgtcggg aacgcaataa acggcggcaa
     2881 cctgcgggct gccttcggtc agcgtaccgg ttttgtccaa cacgacggca tcgacgtggg
     2941 cggcttcctc cattgctgcc gcgtctttaa accaaatacc gtgtttaacc gctttgccca
     3001 taccgaccat aatcgcggca ggggttgcca gacccagcgc gcacgggcag gcaatcacca
     3061 aaacggcgac ggcgtgcatc agcgcaaccg tccaatcgcc cttaatcagc caagtaacaa
     3121 taaaagtcaa caacgcaatg cccacgacgg caggcacgaa taccgcagcc gctttatcgg
     3181 ctacgcgcgc aatcggtgct ttactgcctt gtgcttcaga gagcgcgttc atcatgtcgc
     3241 cgagctgggt ttggctgccg agctgcgtgg cgcggtacac cacactgcct tcggtcatta
     3301 acgcgcccgc caacactttg ccgcccgcct ttttttcttc aggattggat tcgccggtaa
     3361 gatggctctc gtccgcccaa ccgctgccgc tttcaatgat gccgtctgcg gcaatgcgtt
     3421 cgccgtggtt ggcgcggata aggtcgccga tttgcacttg gtcgatggga agctgtttcc
     3481 attcgccgtt gcgttgcacg ttgacttggg ttggtgtaag tttgagcagc aagcccaagc
     3541 tgttgaggct ggattttttg gtacggtgtt ccaaaaattt acccagtgac acaaaaccga
     3601 tcaccatcac gcccacttca aaatacacat gcgccatacc gtacgccgcg tgcgggctga
     3661 aaaacagcat atagacggaa tacaggtaaa tcgagaccgt gccgatggta accagcacgt
     3721 ccatattcgc cagtccgccc ttaatgctcg cccacgcgct tttgtaaaac gggattgcca
     3781 gccaaagctg caccacgctt gccaatgcga actgccacaa cggcggaatc atccaatcgt
     3841 gtctgccgat catcatcccc gccatgccga taaggaacgg gacgttgatg gtgaacagca
     3901 gccacagccg ccagccgata tggtgttctg cttcgggttg cggcaatgta tcttccgttt
     3961 tttccttcgc gccgtaaccg gttttctcaa tgattttggc aatgtcggct actgaggttt
     4021 tgctgtcgtc aaacactacc tgcgcctctt cgctggcgaa gtttaccccc gccgattcga
     4081 caaaatcttt tttgttcaac actttttcaa tgcgcgaagc gcaggcctgg caggtcatgc
     4141 cttcgatttg gaaacggatt ttttgttgca taagcgtgat tcctgctttg atattcagac
     4201 ggcagttaag gctgcgggat gccgtctgag cgccggtatg gtcgaaattt ctcaaacgcg
     4261 ccgataattc gagtagaatc cgaaaacttg acacagcaaa ggaaaaacaa tgtcccaaca
     4321 ccgcaaactg attattttgg gttccggccc cgccggatac accgccgccg tctatgccgc
     4381 gcgcgccaat ttaaaccccg tcattattac aggtatcgcg caaggcgggc aactgatgac
     4441 caccaccgaa gtggacaact ggcctgccga tgccgacggc gtgcaagggc cggaattgat
     4501 ggcgcggttt ctcgcccacg ccgaacgttt cggaacggaa atcatttttg accaaatcaa
     4561 cgccgtcgat ctgcaaaaac gcccgtttac actcaaaggc gatatgggcg agtacacttg
     4621 cgatgccctg attgtcgcaa caggcgcgtc cgccaaatac ctcggtttgc cgagtgagga
     4681 agcgtttgca ggaaaaggcg tttccgcttg tgccacctgc gacggtttct tctataaaaa
     4741 ccaagatgtt gccgtagttg gcggcggcaa tacggcagtt gaagaggcac tctaccttgc
     4801 caatatcgcc aaaaccgtta ccctgatcca ccgccgcagc gagttccgtg ccgaaaaaat
     4861 catgatcgac aaactgatga aacgcgtgga agagggcaaa atcatcctca agctggaaag
     4921 caacctgcaa gaagtactgg gcgacgatcg gggcgtaaac ggcgcattat taaaaaacaa
     4981 cgacggttct gagcaacaaa ttgccgtcag cggcattttt atcgccatcg ggcacaagcc
     5041 gaataccgat attttcaaag gtcagttgga aatggacgaa gccggttacc tgaaaaccaa
     5101 aggcggcacg gcggacaatg tcggcgcaac caatatcgaa ggcgtatggg cggcgggcga
     5161 cgtaaaagac catacctacc gtcaggcgat taccagcgcg gcttccggct gtcaggcggc
     5221 tttggacgcg gaacgctggc tgggcagcca aaacatttga atgccgtctg aaaccgaaat
     5281 tttcggcgga atgcaaattt ttcgcaccaa atccttgatt ccgtcggcaa accccaatat
     5341 aatgccgctt ctttaaacct tgccttctgc tttcgcatgg cagaaggctg tttttaccat
     5401 ccataaggag ataacatgcg tcattacgag atcgtgttta tcgttcatcc cgatcaaagc
     5461 gagcaagtac ccgctatggt tgaacgttac aaaaccatga ttgccgaagc aaacggtaag
     5521 attcaccgtc tggaagactg gggtcgccgc cagctggctt acccgattaa caaaatccat
     5581 aaagcccatt acgttttgat gaacatcgaa accactcccg aagtggttga agagctggaa
     5641 accgcattcc gcttcaatga tgcagtattg cgtcatctga ccatcaaaac caaacacgcc
     5701 gttaccgaag catcccctat gttgggtggt gaaaaggcta agaacctgtt gagcggtgcg
     5761 tctgaagaag cggtcgccca ataattggga ttcaataatc ttgtttcgct tgccgcgtta
     5821 attgaaaagg ttttccctat tcgatatacg cctgccggaa tccctgtttt agatattatt
     5881 ttaaagcacg aatcgtggca ggaggaaaac gggcagcaat gccttgtcca attggaaatt
     5941 ccggcacgga ttttaggcag gcaggcggaa gagtggcagt atcggcaagg tgtatatgtt
     6001 cacgtcgaag gttttttagc tcaaaaaagc agacgttccc ttatgccgat gctcaggata
     6061 caaaatattc aagaatataa aggttaaacg acaatggctc gtcaatcatt caaacgtaga
     6121 aaattctgcc gtttcacggc tgaaaaaatc caagaagtcg attacaaaca agttgatttg
     6181 ctgaaagact tcatctccga aaacggtaaa atcattcctg cacgcatcac aggaacgaag
     6241 gcattctacc aacgccaatt ggctgttgcc gtaaaacgcg cacgcttcct ggctctgctg
     6301 ccttacaccg accaacacaa ataattttgg agattgaatc atgcaaatta ttctgttaga
     6361 aaaaatcggc ggtctgggca acttgggcga catcgtaacc gttaaaaacg gctacgcccg
     6421 caactttcta attcccgcag gtaaggcaaa acgtgcgacc gaagcgaata tgaaagagtt
     6481 tgaagcacgc cgcgcagaac tggaagccaa acaggctgaa attttggcag atgcccgagt
     6541 ccgtcaggaa aaattggacg gtcaaaccgt taccgttgct caaaaagctg gtgtggacgg
     6601 tcgcctgttc ggttccgtta ccaatgccga cattgctgct gcaatcgttg ctgccggcat
     6661 cgaagccgtg aaagcaaatg tacgtctgcc gaacggtcct ctgaaagccg ttggcgagta
     6721 cgaagtggaa gtggctttgc acactgatgc cgttgctaaa attaccgttg ccgtcgttgc
     6781 cgcaaccgag taattgaaat tatcagatgc cgtctgaaac cctgccgatt gtcggaatgt
     6841 ggtttcagac ggtatttgtt ttgtttttcg tatattctgc aaaggtctcg gtcaggttgt
     6901 ctgtcctttt gtcggaacag gagactgggc agctttgtcc tgattaattg cttgtgcaaa
     6961 cggtaaaaag gttttttcgt gtgttttaaa gcaggttgta cagcattttt ccggcaatca
     7021 aaagcaacat aatgccgaag gattttttga gtttggcaga agaaagtttg tgggcggttt
     7081 tgacaccgag cggggcaaag gcaatggttg ccgcgctgag gacggcgacg gcgggcaggt
     7141 aaaggaagcc cagtgaccct tcgggcaatc ctgcaatatt caggccgttg agcagatacg
     7201 atattgcgcc ggagagtgca atcggccagg caaggccgga tgatgtgccg atggctttat
     7261 gggcggggaa gccgcagtgg attaagaagg ggacggaaag tgaaccgccg cctatgccga
     7321 cccagctcga cattgtgccg aacagtgtgg aaaccgcagt cagtccgggc agtccgggca
     7381 gcgggcggga tgccgtctga gggtcggtat gcagtgtttt gaatgcgacg gcggttaaaa
     7441 acaggatgaa gaaaatttga agcccgaacg cggggatata ttttgcggag agtgcgcccg
     7501 tgaatacgcc gaatatcata cccggcatca tcgtaaatac ggttttccag tcgaccgcct
     7561 gttttttgtg ctgccccagc atactggaaa aggcggtgaa gaccatgacg gcgaaggatg
     7621 tgccgacggc gaggtgttgc gcgtaaggat gttgtgccaa accctgcaaa tcaagcaccc
     7681 ataaaacgac agggacaatc agcgtgccgc cgcctacgcc gaacaggccg gcaataaaac
     7741 ctgccgcact gcctacggca agcaggatta agataatgtc ccaatgccac atttaatccg
     7801 cctttctgta ctttttccaa aatcggcgtg ccgccataat gtagccggac aggctgtatc
     7861 cgagaaagaa caggaagagg acgagcgacg gttcccaagt gaccagaagc agcagtaaga
     7921 cggcaagcag cattccgaca aaggggactt gtctgcggat gttgatttct ttaaaactcc
     7981 aaaaagggat ttggacaatc atcgacaggc cggcaaacag tgtgatgccc aatgcccacc
     8041 agtggacggc ggggaatttt tcgacgctgt ggttgaccca aatcagcccg acaatcagcg
     8101 cggcggcagt cggactgggc acgccgataa accagcgttt gtccaccttg ccgatgagtg
     8161 tgttgaacag ggcgaggcgc agggcggcgc aggcgcagta gatgaaggcg acggaataac
     8221 cgattttgcc gaactgccaa agctgccatt tgtaggcaat cagagcggga gcaacgccga
     8281 agctgaccat atcggcaagg ctgtcgagct gctccccgaa cgcgctttgg ctgttggtca
     8341 gccgcgccac gcgcccgtcc ataccgtcca gcaacataga gatgaatacc gcgatggcgg
     8401 cggtttcata acgtccgtgc atggattggg tgattgcgta aaacgcggaa aacagcgcgg
     8461 cgatagtaaa ggaattgggc agcaggtaga tactgttttg acgcagggag cgttgcgggg
     8521 tgttggtcgg ggtttccata gctgaagtgt ctcacggacg tggagaatcg ataggggatt
     8581 ataacccaaa ccattatcga acaatacgcc ctttaggatg aagccctcgg aggcggtttg
     8641 tttttctgat ggcggatgct ttgcggggcg gtttgactct gcctgccgcc gggtgtgttg
     8701 tttcggattt ttgccggatg gatgttgttg ccgggcattt ctgggcttgc cggaaggtat
     8761 cggggggagg gcatcggaat atgcggcgcg ccgttcaaac cggggcggcg tttccctatt
     8821 ttcggggagt ttgcaggctt caacaaattg aaagatattt tttgcatttc cgaccgtatg
     8881 ctgattttga aacgggataa attataggac aaggtttttc gcccgccgca aaggttggcc
     8941 gcaatgccgt ctgaaaacaa cggcttgccg ctaaggcggc ggtatgcgga tttgcgctgc
     9001 tgaaatcggt ttgccatacc cggcggtgca atctgcgggg agggataagc cgataaggcg
     9061 ggacttatcc tgttttttgt ttataatgca acagaatttg gacgaaccgt cgggaattta
     9121 tcatgatgaa aaagatactg gccgtatcgg cactatgcct gatgactgcg gcggcacggg
     9181 ctgccgatac ttacggctat ctcgccgttt ggcagaatcc gcagaatgcg gacgatgttt
     9241 tgcaggttaa aaccacaaaa gaagattcga cgaaaagcga agcgttttcc gagttggaag
     9301 ccttctgcaa aggtcaggac acgcttgcgg gcattgctga agacgagccg accggatgcc
     9361 ggtcggtcgt gtcgctgaac aatacctgtg tcgcgctggc atacccgaaa gccttgggcg
     9421 cgctgcgtgt cgacaacgcc gtcgtgatta cttctccgcg ttttacgagc gttcatcagg
     9481 tcgcactcaa ccagtgcatc aaaaaatacg gcgtacaggg acaatgcggc ttggaaacag
     9541 tgtattgcac atcttcttct tattacggcg gaactgtgcg ctctttgatt caaaatctca
     9601 aataaaacgg aaaatgccgt ctgaaagatg ttcagacggc atttctatat cgacggtcag
     9661 gattctttcg gatcgggcag caggctgttc aacataatgg caagtacggc gcacaagccc
     9721 acgccggcaa agctgaagct gcccaatttg agcgtcatgc cgccgatgcc cgtggtcagt
     9781 accgagctga cgatgaccag gtttttcggc agcatcaaat cgactttggc atcaatcagc
     9841 gttttcacgc ccaaagaagc aatcgtgccg aacagcagca gcataatgcc gcccattact
     9901 ggcatcggaa tggaagccaa aaacgcattg aatttgccga aaaacgccat gcagacggca
     9961 aaaaccgccg cccaagtcat gatgacgggg ttgctgtttt tggtaatcat caccgcaccc
    10021 gttacttcgc cgtaggtcgt aaccggcggg ccgccgatca gacccgcaac gcatacgccc
    10081 aaaccgtcgc ctgcaagggt tttgtccaag cccgggtctt tcgtatagtc tttccccgtc
    10141 acattgccga ttgccatgat gccgccgatg tgttcgatgg cgggggcgac ggcaacgggc
    10201 agcataaaca gtgcagcctg ccagttgatc tgaggcgttt caaaatgggg aacggcgaac
    10261 cagggcgcgt gtgcaatgct tgccgtgtcc accagtccca tcagcagtgc caaaacataa
    10321 cccgaagcga caccgatcaa gatgggaatc agcttcatca tcctgctgcc gaaaaccgat
    10381 acgatggcgg taacggcaaa ggtaaagccg gaaagaatca gcgaatcggt atagtcgatg
    10441 acctgtttgc cgtccgcctg acccattgcc atgctgcttg ccgccacggc aacagacaga
    10501 ccgatgacca tgatgacggg gccgatgacg accggaggca gcagtttgtg taccgctgcc
    10561 agtccgcgcc aacggatcag cgcggcaaac acaaaataca taaagccggc ggcaaacagt
    10621 ccgaacatgg tggaaggcag cccccattcg ccgacggagt agataatcgg tgcgataaag
    10681 gcaaacgaag aaccaagaaa aatcggcact ttgcgtttgg ttgtgatttg gaacagcagc
    10741 gttcccaagc ctgcgcccaa aagcgcaaga gccggattca gaccggtcag caggggaacc
    10801 agcaccattg cgccgaatgc cacaaataaa atctgtgcac cggaaacggc aagtttcagt
    10861 tggttcatac catcttcttt caatcggttc aaacggcacg gattatactg gtttgccgag
    10921 ggtctgcgta aaaatacata cgtcgggcaa cagatatgcc gtctgaaagg caatgtctgt
    10981 ctgttgcagg atgaaagcag gagaaatgga gtataatccg acactttcca tattcaatcc
    11041 gcaagccgtc cggcttcctt atttcagacg gcattttccg gtaatacggc atgacagaaa
    11101 aatcccataa aaaaacagca aaaggcaggg cgggaagccc gtccccaacg tccgcacgca
    11161 acaagaaagc cgacaacggc gcacggggca ataaagtttc cgagcggctc aaggcggtca
    11221 aagagttgca gaaaaccgaa accaaaaagg cgcgtcccga acatgtcgtc aaccttatcg
    11281 gcgacgcact gtggctgatg ggtttggcgg caaccctgta tttggcgatt tccctgatca
    11341 gtttcgatat gggcgatccg tcttggtcgc acagttcgcc ggttgtggaa gatgtcgcca
    11401 attggggcgg actgttcggc gcgtatgttg ccgatgtcgg ctattatctt ttcggctggt
    11461 cgttctggtg gtggatagcg gctgcctgcg tcgtgctgta taaaaatttc cgcctgcacg
    11521 caaaacagac ggaaaacgag gcatacaacc acaaaatcgc tgccgccgcg ctgtttgtcc
    11581 tgacggtctt cagccccgtc ttggagtatt ttgtgctggg cggaaaatat gccgactccc
    11641 tgcctgtcgg agcaggcggt atggtcggca tacgcgtcgg cgcagtgttt gcgtggctgc
    11701 tggggaaatc gggcagcctg ctgattatct tggttgttct gctgttgtcg ttgtccctgc
    11761 tggtgcagat ttcatggctg gaatttttga acggtgcggg cagggcggtt caaaaccgcc
    11821 tgagtgcctt atccggcaag gtcatggctt taggaaaacg ccggccgaat accaaaacag
    11881 acggtgtcga tacccaaaat acacggcgca tggtaaaaga agccaagaat attacggcca
    11941 aacccgttgc cttgcccgaa ggcagcagca gcaaccgcaa atccgtcgcg gtttccgtcg
    12001 cgccgccgcc caaaattcag gtttctctgt ttgaagatga cgaacctcgg caggcgggcg
    12061 aataccacaa gcctacattg aacctattgc ggattcctga cagcgaaccc gtcagcatca
    12121 atcccgccga attggagcgc actgccgaac tgatcgaatc caaactggca gaattcggca
    12181 tcggcgtaca agtcgtatcc gccacatccg gccccgtcat cacgcgctac gaaatcgaac
    12241 ccgcgcaagg tgttaaaggc agccaaattg ttgccttgtc gaaagatttg gcacgctcta
    12301 tgtcgctgca gtccgtgcgt atcgtcgaaa ccatcgcagg taaaaacacg atgggcatcg
    12361 agttgcccaa cgacaaacgc caagacgtga tgttgagtga aatcttgtcc tcgcccgtgt
    12421 ttgccgaagc caaatccaag ctgaccgtcg cgctgggcaa agacattgcc ggtacccccg
    12481 ttgtcggcga cttggcgaaa atgccgcacc ttttggtcgc cggtatgact ggttcgggca
    12541 agtccgtcgg cgtgaacggc atgattatgt ctatgctttt caaagctacg cccgacgaag
    12601 tccgcttcat tatgatagac ccgaaaatgc tcgagttgag catttacgac ggtattccgc
    12661 acctgctctg tcccgtcgtg accgatatgc gcgaagcagg gcaggcgttg aactggtgcg
    12721 tcgccgaaat ggaaaaacgc taccgcctgc tttcccatgc cggtgtgcgt aatttggagg
    12781 gcttcaacca aaaagtcgaa gccgcaaaag cggcaggcaa gccgctgctc aatccgttca
    12841 gcctgaaccc cgacgagccc gagccgctgg aaaaactgcc gttgattgtg gtcgttatcg
    12901 acgaacttgc cgacctgatg atgaccgaac gcaaagccgt cgagcagcaa atcgcccgtc
    12961 tcgcccaaaa agcgcgcgcc gccggtatcc atatgattgt cgccacccaa cgtcccagtg
    13021 tcgatgtcgt taccggcctg attaaagcca acatcccgac gcgtatggcg tttaccgtgc
    13081 aaagcaaaat cgacagccgt accatcctcg accaaatggg cgcggacgaa ctgctcaaat
    13141 atggcgattc gctgttcctc cagcccggca gtgccgaacc gactcgcctg caaggcgcgt
    13201 ttgtttcaga cgacgaagta catcaagtcg tcaactatgt caaatcgcaa gccccagccg
    13261 actatattga aggtctgctc agcggcgagg ccgcgctgga aactgccaat atcgttaatc
    13321 cgaatgcaga cagcgacgaa ttgttcgatc aggcagtcgc ctatgttttg gaaagcaaaa
    13381 aaacctccat ttcgtctttg cagcggcagc tgcgcatcgg ctataaccgc gcggcaaacc
    13441 tgatggaggc actggaaaat gcgggtgtcg tttcttccac cgacctcaac ggcagccgta
    13501 aaattttggc gcacaaggac catttgtagc ccgtattgca aaatgccgtc tgaacggcgg
    13561 aattggcgtt tcagacggca tattatgttt caggcgaaac attgtgatat acttgccagc
    13621 taaaatttcc cctttgcggc aatgcggttc aaatatcgta ccgttgcgct gtttgcttcc
    13681 ccatgtaggg aagaaagttt atcattttat caacacaaca aatttaaggg cttatgatga
    13741 gcgtaactgt tgaaacttta gaaaatctgg aacgcaaagt agtgttgtcc ctgccttggt
    13801 ccgaaatcaa cgcagaaacc gataaaaaac tgaaacaaac ccaacgccgt gcaaaaatcg
    13861 acggtttccg tccgggtaaa gcacctttaa aaatgattgc ccaaatgtac ggtgcgagcg
    13921 cacaaaacga cgtgatcaac gagctggtgc aacgccgctt ctacgatgtt gccgttgccc
    13981 aagagttgaa agtggcaggc ttcccccgtt ttgaaggcgt tgaagaacaa gacgataaag
    14041 agtctttcaa agttgccgcc atttttgaag tgttccccga agtcgttatc ggcgatttgt
    14101 ctgcacaaga agttgaaaaa gtaaccgctt ccgtcggtga tgccgaagtg gaccaaaccg
    14161 tagaaatcct gcgcaaacaa cgcacccgct tcaaccatgt cgaacgcgaa gcccgaaacg
    14221 gcgaccgcgt catcattgac tttgaaggca aaatcgacgg cgaacctttt gccggcggcg
    14281 catccaaaaa ctacgccttc gtattgggcg caagtcaaat gctgcctgaa tttgaagccg
    14341 gcgtagtcgg catgaaggct ggcgaaagta aagacgttac cgtcaatttc cctgaagact
    14401 accacggtaa agacgttgcc ggtaaaactg ccgtgtttac cattacgctg aacaacgttt
    14461 ccgaagcgac tctgcctgaa gtcgatgcag attttgcaaa agccttgggt attgcggatg
    14521 gcgacgttgc caaaatgcgc gaagaagtgc agaaaaacgt aagccgcgaa gtggaacgcc
    14581 gcgtaaacga acaaaccaaa gaatccgtaa tgaacgcgct gctcaaagcc gtagagctga
    14641 aagcacctgt tgctttggtc aatgaagaag ccgcacgctt ggcaaacgaa atgaaacaaa
    14701 attttgttaa ccaaggtatg gctgatgctg ccaacttgga tctgcctttg gatatgttca
    14761 aagaacaagc cgaacgccgc gtatctttag gtctgatttt agccaaactg gttgacgaaa
    14821 acaaactgga accgactgaa gagcaaatca aagccgttgt tgccaacttt gcagaaagct
    14881 acgaagatcc tcaagaagtg attgactggt actacgcaga tccttcccgc ctgcaagccc
    14941 cgacttcttt ggcggtagaa agcaacgtcg ttgatttcgt tttgggcaaa gccaaagtaa
    15001 atgaaaaagc tttgtctttt gacgaagtga tgggcgcgca agcctgatta tcctgaaaat
    15061 gccgtctgaa ggcagttttt gaaagcaccg aagccgcttt taaagctcgc tttggtgctt
    15121 ttccatcatg aaaggagacg aaatgtcttt tgataactat cttgtcccta ccgttatcga
    15181 gcagagcggt cgcggtgagc gtgcattcga tatctattcc cggcttttga aagagcgcat
    15241 cgtattcttg gtcggaccgg taaccgacga gtccgccaat ctggtggttg cccaactgtt
    15301 gtttttggaa agtgagaatc cggataagga tattttcttc tatattaact cgccgggcgg
    15361 ttcggtaacg gccggtatgt cgatttacga caccatgaat ttcatcaagc ccgatgtatc
    15421 gactttgtgc ttggggcagg cggcaagtat gggcgcgttc ttattgtcgg caggcgagaa
    15481 aggcaaacgt tttgccctac ccaacagccg gattatgatt caccagcctt taatcagcgg
    15541 cggtctgggc ggtcaggcat ccgacattga aattcacgca cgcgaacttt taaaaatcaa
    15601 agaaaaactc aaccgcctga tggcgaaaca ttgcgaccgc gatttggcag atttggaacg
    15661 cgacaccgac cgtgataatt tcatgtctgc cgaagaagcc aaagaatatg gtttgattga
    15721 ccagattttg gaaaaccgcg cttctttgcg gctttaataa aagaaacctg agagaaaccg
    15781 atgccgtctg aaacgttcag acggcatttg tttcaagctg cgtttgatat ggtatcgata
    15841 ccgtcatcca cgggacggaa gaaggatttg gagtttttta aaggatcccc gttcccgcct
    15901 gtgatggacg gatgccgtct gaaagccaaa tggggcagca gtttttgcaa gggcgcgaca
    15961 ctcgcatcct cctgaatctg ccggtacggt tattgagaaa agacaagggc aggaagcggt
    16021 atcccgcctt ccttatttat acctgcaatt catctccgta tttgccgatt gtattttcat
    16081 attgcgcctc cattccttat cggtttaaaa aaatgccttt tccaatggga tgggttttat
    16141 atcaatgaaa ataaacggtt tttatttaaa aatagccaag gtaggctggc ggttggattt
    16201 tatagggagt gtatcgggat aaccttccct tgatggcgtt tgagtccgtt ctccccataa
    16261 ttggttttga catactcttc aacaatagcc agaaattccg ttgcaatgtt gtcgcctttc
    16321 agcgttactt tacgttcacc atctacataa acaggggcaa cgggtgtttc tcccgtaccg
    16381 ggcaggctga tgccgatgtc ggccaatttg ctttctccgg gcccattgac aacgcagccc
    16441 attacggcaa cgttcaggga ttcaacccca ggataaaggg tacgccatat agacattttt
    16501 tggcgcaggt aattttgaac atcttgtgcc agctcttgaa atacggtact ggtggtacgc
    16561 ccgcagccgg ggcaggcggt aaccatcggc gtaaacgaac gcaatcccat agtctgtaaa
    16621 atctcttgcc cgacgacgac ctcctgagta cgcgggctgc caggttccgg agtcagtgaa
    16681 atgcggatgg tgtcgccgat tccttcttga agcaagacgg ataatgccgc cgttgatgcg
    16741 acaatgcctt tgctgcccat accggcttcg gtcaaaccca aatgcagcgg ataggcgcaa
    16801 cggctgccca gttcgcggta aacctgaatc aaatcctgaa ccgcgctgac tttgcacgac
    16861 aggatgattt tgtcttcggg cagtcccaat agaacggctt tttcggcaga ttccaaagcg
    16921 gagacaatca gtgcttcctt cgtcacttct tcgggcggtt tcggcgcgga agaagcgagg
    16981 ttggcatcca tcatacgttt ggcgaggctt tgatccaaag aaccccagtt tacgccgatg
    17041 cggacggctt tatcgttttc agcagcagtc cgaatcataa aggcaaattt ttcatcgcct
    17101 tttacgcctt tgccgacatt gccgggattg atgcggtatt tggacaatgc tttgccgcat
    17161 tctggaaatt ccgccaacag gcgttcgccg ttgaagtgga aatcgccgat aagcggtgtg
    17221 gcatagccca tatcgtccaa gcggcggcgg atttcggcaa ctttggacgc ggcttcgggg
    17281 ctgttgacgg taatacgcac catttcggat ccggcatcgc tcaattcctt aatctgcaat
    17341 gcggtggctt ttgcatcggc agtgtcggtg ttggtcatag attggataac gacgggtgct
    17401 tctgaaccga cggtaatatg atcgatgcgg acttgatgcg tcttgcggcg ttggagtgtg
    17461 ttcatatggt ttgaatctgt ttattgaccg gtgaggacgg tttgcaattc ttccgagtag
    17521 gggaaattcg cctgcaattg tgcttcatat tcgtatgccg cctgtgcgtt gccgagggct
    17581 ttggcaattt tccagcctag cagcaaatca tcggcctgaa ggacttctac cctgctttgg
    17641 tattttttaa agtagtaatc ggcatcgccc aactgcccgg ccagcatttt ggtgcgcgcc
    17701 agttctttaa atgcgggtgg gaactgcggc tgggcggcga gggaacgttt caaataggct
    17761 tccgccaatc cgaattgccc ctgttttgcg ctgcatatgc ctttattcag gttggcaata
    17821 taaggggtcg ggtaggtggg gtcggccaga gctttgtcga aatatgccat agattcggca
    17881 gggcggttga gcctgccgca taggaaccaa ccgtagttgt tgttgatttc ggcactgtcg
    17941 ggtttgatgg agagggcttg ccggaaactt tcctgcgcct tgtcgttaac tttcaggtat
    18001 tgatagattt cggcacggac cagccaggca agctcgtttt tagggtccga tttcagggcg
    18061 tcttcaatac ttgccgtcgc ctgacggtag tcctgaccgc gcatatattc cattgccaac
    18121 tgggttttga tattggaaac ctgattggct ttttctgccc gcgaggggcg gtaggaagtg
    18181 ctgcacgcgc ccaaggcaag aacgagtaat aaagagattc gtttggatgg cttaaaaggc
    18241 ataattaccc ctgttgtccg attaaaatct gctgccattt ttgttggcgg cgcgttttat
    18301 cctgaacctg ccccgccaac tgtccgcagg cggcatcgat gtcgtcgccg cgcgtttttc
    18361 gtacggtaac gacaaatcct gcctgctgca aaatatcgcg gaacacacgg atgttctcat
    18421 tgctggagcg ttcgtatccg gagtttggga agggattgaa cggaatcaga ttgaacttgc
    18481 agggaacatc tgtgaccagt tcgatcagtt cgcgcgcatg ttgcgcctta tcgtttattc
    18541 cgtccaacat gacgtattcg aaagtgatga aatccctggg tgctttgacc agatagcgtt
    18601 ggcatgcggc catcaattct ttcaagggat attttttgtt caacggtacg atttggttgc
    18661 ggacttcgtc attggaagcg tggagggaaa ccgccaaagc caccggcatg acatcgcgca
    18721 acctgtccat ttggggaacc atacccgaag tggaaacggt tacgcggcgg cggctcaaac
    18781 cgtagccgtg gtcgtccagc atgatgctta aggcggtaac gacattgtcg aagttcgcca
    18841 tcggctcgcc catgcccatc atgacgacgt tggaaatcac gcgctcgttt ttcggtgtaa
    18901 cgcccatcgc tttgtttgcc caccacaatt gcccgatgat ttcggcagca gtcaaattgc
    18961 ggttgaagcc ctgccggccg gtcgaacaaa atgtacattc caaagcgcag ccgacttgtg
    19021 aggaaatgca gagcgtgccg cgatccgatt cggggatgaa gacggtttcc acgccgttgc
    19081 ccgtaccgac atccaaaagc cattttcgag tgccgtctga agatttttga gacatcatca
    19141 gcttgggaat ttcgatgcct gcctgttcgt tcagtttatg gcgcaacgat tttgccaaat
    19201 cggtcatttc gtcaaaattt tgcgcgccgg attggtgcat ccaacgcata acctgtttgg
    19261 cacggaaagg tttttcgccc atatcggcaa aatgtcgggt cagcccttga aggtcgtagt
    19321 tgagcagatt ggttttcatg tgtttgtgtt tttcttaaat caaggcttca aataaaaaat
    19381 tgcagggcag gcaagtttga tttgaagcct tttgcaacag agttttcaga cggcatggat
    19441 atatttatgc cgtctgaaga ggagttgggc ggtgtattgt atcaacgggg gcagatttcg
    19501 gtttggctga aaaagtaggc aatttccaaa gcggcatttt ccacgctgtc ggaaccgtgt
    19561 acggcattaa tgctgaccga agtggcaaag tccgcgcgta tcgtgccttc ggcggcttcg
    19621 gaaggattag ttgcacccat cagttcgcgg tttttcagga cggcgttttc accctctaat
    19681 acctgaatca taaccggacc gccggtcata aattcaacca atccggcgta gaaggggcgg
    19741 tctttatgaa ccgcataaaa ttcttgcgcc tctttgagag taagctgctt cattttggcg
    19801 gcaacgattt tcagaccgtt ctcctcaaag cggctgtata ttttgccgat aacatttttg
    19861 ccgacggcat cgggtttgat gatggagatg gtacgttcaa tcgccatgct atatccttat
    19921 ttttacttaa gaagaatcaa atcggtattc tatcaaaaaa ataacttatc cgcctgaagc
    19981 cttgggagat agggtcaggt gcggctgttc caaataaact tcggactgca tttcgaccat
    20041 ccggctggcg gtgcgggtaa attcttcggc aaaatcgcct tccgtataga tatggttgac
    20101 atctaccgcg ccggtggcac acagtttgac ccggaaatcg tagagtacgt caatcagcca
    20161 agtcagccgc cgcgcctccg ccttttcttg cggtgagagt tgttccaaac ctgaaataaa
    20221 aaccatttca taatgttcgg ccaaatacag atagtcggac tgtgagcggg ggccgaagca
    20281 cagtgcgcgg aaatcaaacc atatggcacg gccggactcg gctttgtggg gaatctcccg
    20341 accgtggatg gtgctgatgc cggggttcaa atcggtaatg cctgtcattt ctttgaacag
    20401 ttttgccagt tttgcctcat tttcttcatt ggcaggcgta aagaaaatct cggcggggcg
    20461 gagggtacgc agtcggtagt cttcaccgcc gtcaacgttt aagacggtca ggctggactc
    20521 gatgagcgcg attgtgggaa gaaaactgct ccggttttga ccttgcgggt agagttcgga
    20581 aggcgcgtag tttgaagtcg ccaccaaaac aacgccctcg ttaagcaggt tttccagcag
    20641 acggcctaaa atcattgcat ccgcaatatc gctgacatga aattcgtcaa aacacaatac
    20701 gcgggtttct ttggcaatct cggcggcaac ggatttcaac gggttgcttt cgcttttcag
    20761 ggttttcagc cgctggtgga tttctgccat aaaggcatga aagtggacgc ggcgtttgcg
    20821 gcggtacggg aggcagccga aaaaagcgtc catcagaaag cttttgccgc gtccgacccc
    20881 gccatagaaa taaagccctt tggggacttg cggggaacgc aaactcctgc ctaaaaaacg
    20941 gtttcttttg cgtttgaaca tcatcaattc ggtccaaagc cgatcgaggt gttcgatggc
    21001 ggctgcctgc gcgtcgtcgc ggatgaagtt gggcagttgt gaggcagcct gataccaggt
    21061 cagcgggctg tggttttcaa acggcggggc tttaaaaagt tggtctctat tactcatatt
    21121 taaccttgtt atacttcttg cggctaagaa agaaaatgct taccgtcggt attataaatg
    21181 atttccttca aacttggcat tttccgtgga aaagaataat gacgcgcaca agggggtata
    21241 agcaaaggcg gcgtttcaat atggtgcaga tatgagctca aatgcctttt atagt
//
LOCUS       DAWGRZ010000014        21101 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000014 DAWGRZ010000000
VERSION     DAWGRZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21101
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.014"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(23..714)
                     /locus_tag="WHT44_000376"
                     /pseudo
     CDS             complement(23..714)
                     /locus_tag="WHT44_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1020..1577)
                     /locus_tag="WHT44_000377"
     CDS             complement(1020..1577)
                     /locus_tag="WHT44_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532611.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWGYHGANLYNHGKLGTAQGAATATGIGAATGVA
                     AKQGWLLLPEADWLGIWHGDRVFMH"
     gene            complement(1821..1979)
                     /locus_tag="WHT44_000378"
     CDS             complement(1821..1979)
                     /locus_tag="WHT44_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532612.1"
                     /translation="MKTPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(1976..2818)
                     /locus_tag="WHT44_000379"
     CDS             complement(1976..2818)
                     /locus_tag="WHT44_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ2532613.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPILVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3057..3977
                     /gene="pyrB"
                     /locus_tag="WHT44_000380"
     CDS             3057..3977
                     /gene="pyrB"
                     /locus_tag="WHT44_000380"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ2532614.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWNCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            3987..4445
                     /locus_tag="WHT44_000381"
     CDS             3987..4445
                     /locus_tag="WHT44_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ2532615.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4510..5175
                     /locus_tag="WHT44_000382"
     CDS             4510..5175
                     /locus_tag="WHT44_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532616.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQILAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5238..6455)
                     /locus_tag="WHT44_000383"
     CDS             complement(5238..6455)
                     /locus_tag="WHT44_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ2532617.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            6597..7142
                     /gene="def"
                     /locus_tag="WHT44_000384"
     CDS             6597..7142
                     /gene="def"
                     /locus_tag="WHT44_000384"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ2532618.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            7229..8155
                     /locus_tag="WHT44_000385"
     CDS             7229..8155
                     /locus_tag="WHT44_000385"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ2532619.1"
                     /translation="MKVIFAGTPDFAAAALRAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVEQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSKS
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGAKL"
     gene            8234..9493
                     /gene="rsmB"
                     /locus_tag="WHT44_000386"
     CDS             8234..9493
                     /gene="rsmB"
                     /locus_tag="WHT44_000386"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ2532620.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLMAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIGNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLVASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLVAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASTACADAQDLSAWYDGKPFDAVLADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQVLKSGGRMLIATCSVFVEENDGQLQKFLNRHA
                     DAELIESRVLLPNKHQDGFYYALIQKQ"
     gene            9459..10055
                     /locus_tag="WHT44_000387"
     CDS             9459..10055
                     /locus_tag="WHT44_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ2532621.1"
                     /translation="MAFITRLFKSSKWLIVPLMLPAFQNVAAEGIDVSRAEARITDGG
                     QLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLPAPIIASYRFKLGQLIGDDDNIDY
                     KLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAEI
                     RLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10055..12175
                     /locus_tag="WHT44_000388"
     CDS             10055..12175
                     /locus_tag="WHT44_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ2532622.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSRMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAVPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHKLDQPFPGKARWEKIQRAGSVRDLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATL
                     LIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAE
                     QILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALI
                     GDVLALYEAGPCRFAAELAGEPLTVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVK
                     SETGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLSVVKKIIEEHGGRI
                     SLSNQDAGGACVRIILPKTVKTYA"
     gene            12168..13445
                     /locus_tag="WHT44_000389"
     CDS             12168..13445
                     /locus_tag="WHT44_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ2532623.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGAIDF
                     LEKPISLQKLLSAVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVGAAVKCASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPASFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAGAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSQ
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13536..14729
                     /gene="dprA"
                     /locus_tag="WHT44_000390"
     CDS             13536..14729
                     /gene="dprA"
                     /locus_tag="WHT44_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ2532624.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRRFGSAQNALSAPAEQV
                     AALIRHKQALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVQLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGKGIPVVSGMASGIDT
                     AAHQGALQAEGGTIAVWGTGIDRIYPPVNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITARLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKGIPEKRITAVQTASDQLSLPEGKMPS
                     EKTENRPVGGSILDRMGFDPVHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRY
                     QRIRT"
     gene            14755..15216
                     /locus_tag="WHT44_000391"
     CDS             14755..15216
                     /locus_tag="WHT44_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ2532625.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15288..17594
                     /gene="topA"
                     /locus_tag="WHT44_000392"
     CDS             15288..17594
                     /gene="topA"
                     /locus_tag="WHT44_000392"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ2532626.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCVNYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECLNCHWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17667..18362)
                     /locus_tag="WHT44_000393"
     CDS             complement(17667..18362)
                     /locus_tag="WHT44_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532627.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFK"
     gene            complement(18382..18933)
                     /locus_tag="WHT44_000394"
     CDS             complement(18382..18933)
                     /locus_tag="WHT44_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532628.1"
                     /translation="MEFLSLHPWWSFFIILTIILSYIGFCAHLHIQNRAVFFYSYRDV
                     TIIFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIITYRSNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            19052..19429
                     /locus_tag="WHT44_000395"
     CDS             19052..19429
                     /locus_tag="WHT44_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ2532629.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            19518..20087
                     /gene="rsmD"
                     /locus_tag="WHT44_000396"
     CDS             19518..20087
                     /gene="rsmD"
                     /locus_tag="WHT44_000396"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ2532630.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVFSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PNIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            20210..20461
                     /locus_tag="WHT44_000397"
     CDS             20210..20461
                     /locus_tag="WHT44_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ2532631.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            20558..20641
                     /locus_tag="WHT44_000398"
     tRNA            20558..20641
                     /locus_tag="WHT44_000398"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20592..20594,aa:Tyr,seq:gta)
     gene            20672..20745
                     /locus_tag="WHT44_000399"
     tRNA            20672..20745
                     /locus_tag="WHT44_000399"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20704..20706,aa:Gly,seq:tcc)
     gene            20755..20829
                     /locus_tag="WHT44_000400"
     tRNA            20755..20829
                     /locus_tag="WHT44_000400"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20787..20789,aa:Thr,seq:ggt)
     gene            20878..>21101
                     /locus_tag="WHT44_000401"
     CDS             20878..>21101
                     /locus_tag="WHT44_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ2532632.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETR"
BASE COUNT         5319 a         5346 c         5731 g         4705 t
ORIGIN      
        1 taaatgggca aaaatcggtt tattatcgtt tttgccgcat ttggatttgt tctaccgtaa
       61 aacgtgtttg acgaacggga ttcttattaa aaaacatctg atttctaaca aaatcagtat
      121 tttttggcac gatggctaaa atttttcctt ccatttcgcc atcacgtgtt ttccatgcgc
      181 tcaagaattg tgatttgctc attgagacgt gccccagcga tggatcagcc agcaaaacag
      241 tttctccgtt aataccgttc aataccgaaa aatggttgtt tttacggtat tttaaataca
      301 caattacagg aatttttagt tgtaccaact gttcaaatgg caaagcataa ccttgtgctt
      361 caaaacccag ttcgggcatt atgcgttgca tatcgtcaaa agaagcacgc atttgggttt
      421 tatccatttt gtctaagatt tccgcttcag aataatgtct gccataaaaa ttattcagta
      481 acgtggcaat cgaagccgcg ccgcaagaaa aatccaaatc ttgttttact atgccggaat
      541 ctcgccgtgc tttccaactc cgtacatgga tgttttggta agaagcgggg ggtcaacaaa
      601 cataggccaa gcaaaaacta tatttggggc gaaaccaatc aaagccgcat aatttatcaa
      661 tttataaaga ttttttatca taatatgtat acgcggaata aacgcatgaa acataaaaaa
      721 ataaaatcat atgcaatttt attgcatgaa taaatatgaa taaaatagat aatgatggga
      781 gtaaatacgc catgtatttt ggaagtttaa atttattaat aataaaataa tttatcgtag
      841 cgcaaataaa tcccaaaatt ggaacgatta gaaaaaaaat taatacaccc atcatcatgc
      901 aaactctata ttaataaata gctaactaat tctattgtgg aaattagtta gctaactaaa
      961 agttattaat gattattttc gagaattgac tgcattgttg gcagcattgg caccaaaacc
     1021 tagtgcatga atacccggtc tccatgccaa attcccagcc aatccgcctc cggcagcagc
     1081 agccagccct gttttgccgc tactcctgtt gccgcaccga ttcctgtcgc agtagccgcg
     1141 ccttgcgcag ttcctaattt accatgatta tacaaattag caccatgata cccccatgca
     1201 cctaatgcac cgccaaaagc agcggctgca ataatgggaa caaattcacc ttgtgtttct
     1261 ttcatttcag cctgtgataa ttgaattgct ttcacatttt ggctgtcaaa aacttggctg
     1321 tctaaatttt gcgccattac aggtgtaatc atcatagcca ttacagttgc aattttcgtt
     1381 gcgctggttt gcacataaat aggattagca aattcgcttt gattgcgttc agtgttgatg
     1441 tagctaatac tgctttctag tttgaattta cccttgtcag tcaataattc ttccaaactt
     1501 aaaggtaagt cagcataagc aatttggctg gcaacagtca aaataaaatc tattagacat
     1561 ttgtgttttt gcatcatttc gtttgatttt ctaggttttg agaatgatac aaagtttttt
     1621 acaaagtaaa gagtcactct gaaaaaactt ttttcattat aaatcaaaat attgatagaa
     1681 taaatagcga gcatcgattc acggtgcgct ttagtgcaaa gcgtacaacg cgagcctgaa
     1741 ccaccagccg caacaggaaa agaaagccga tagagtgcaa tgcttgccaa cgtgcaagcg
     1801 agcttgcaag aacgcttggc tcaacgagag caggcaagac agaaagcaga aagcaggata
     1861 ggagcggtaa cgcaaaggtc tcgggctttg atttcgccgt aaaccctgct gccgccttgt
     1921 ccggaaaggg tgcaggcggc gagtgccgac agggtgcaga tggggagggg ggttttcatt
     1981 tggggtcgca acggaagtgg tatgcgcaga tttcaaaacc gtttttgaaa tacaggcggt
     2041 gcgcgtcggc acggtcgtgg ttgacgtgga cgttgaggtg gattttggtt acccctgttt
     2101 ccgcgccgat tttgcggact tcttccaaaa ggcgcgaggc gtagcctttg cggcggcttt
     2161 gcggcagggt aacgatgtca tcgatgtgga tgtggcggcc gctggcgagg gtgcaggctt
     2221 cgcggaagcc gcagacggcg acggcattgt gtttgccttc ttcaaaaata cccagcaggc
     2281 ggtagccttg ggggcgttgg actttgttga tctgttcggt aaagcggttg atgtcggtca
     2341 gggcggaacg caaaacgctc aaggctgcaa aggcggtggc ggtgtcgtcc gcgccgattt
     2401 cgcgcaaaac gtaggatgcg cccgaggcgg tctgttcctg tgctttctcg gcggcgtgtt
     2461 tttcttcgat tgcctgtgcc agcatgacgt gttcgtcggc agggttgttt tgtccgccct
     2521 gttcgcgttc ttcgagcagg gctttgcagt cgatgacgcg caggtcgttg tcggcggcaa
     2581 agtccatcag gaagcggaac atttggggat tgtcggtttc cagtttttta tcgacggcga
     2641 cgcagcggat gttgtccacc agtatggggc gcacccattt cgaataggaa agcctgtggt
     2701 ctttggtgaa cgaggacaga atgccgcaca ggcgttccgc ccagtcgctg ggacggaaaa
     2761 tcttgccgga actcgttgtg ccgtggatga cgacttcgta ggggttgcag actaacatgg
     2821 cggcttcctg aaaagaaatg tctagcgcga ttatacctta tgcttatgcg ggcgtgtttg
     2881 gatatgccgt ctgaaaagta cgggattcgt gcggtaaaac tttgcggcgg caaatgtgcg
     2941 ataatacgcg ccgtattgcc gcttttgcga agctgttccg caaacatacg ggcggcgtgg
     3001 acgacgtata accggatacc cgcctgacgc gggtttttta cggaaggggg gcaaaaatgc
     3061 ctaatccgct ttacagacag catatcatct ccatttcgga tttgtcgcgc gaacagttgg
     3121 aatgcctgct tcagacggca ttgaagctga aggcgcatcc gcgcggcgac ctgttggaag
     3181 gcaaacttat cggttcgtgc tttttcgagc cgtccacgcg cacgaggctg tcgtttgaaa
     3241 cggcggtgca gcgtttgggc ggcaaggtca tcggtttctc ggacggcgcg aataccagtg
     3301 ccaaaaaagg cgagacgctt gccgataccg cccgcatcat ttccggatat actgatgcta
     3361 tcatccaacg ccaccccaaa gacggcgcgg cgcgcgtggc agcggagttt tcgcgcgtcc
     3421 ccgttatcaa cgccggcgac ggcacgaacc agcaccccag tcagacgctg ctcgacctgg
     3481 ttaccattta tgaaacacag ggacgtttgg acaagctcaa aatcgccatg gcgggcgact
     3541 tgaaatacgg acgtaccgtg cattcgcttt gtcaggcgtt gaaacgctgg aattgtgaat
     3601 ttgcctttgt ttcgccgccc agcctagcca tgcccgacta tattaccgaa gagttggacg
     3661 aagccggctg ccgataccgt atcctcggta gtttggaaga agcggcggaa tgggcggata
     3721 tcctgtatat gacccgcgtc cagcgcgaac gtttcgacga acaggaattt gccaaaatcc
     3781 aaggcaaatt caacctcgaa gcgtctatgc tcgcccgcgc caaaccgaac ctgcgcgtgc
     3841 tgcaccccct gccgcgcgtg gacgaaatcc atcccgatgt cgatgccacg ccgcacgcct
     3901 attatttcga gcaggcgacc aacggcgttt atgcgcgtat ggcgatattg tcgctggtgt
     3961 tgaacgaaga agtgtgagga accgatatgg aaaccccgaa actcagtgtc gaagccattg
     4021 aaaaaggtac ggttatcgac catattcccg ccggcagggg gctgaccatc ctgcgccagt
     4081 tcaaactttt gcactacggc aacgcggtaa ccgtgggctt caacctgccc agcaaaaccc
     4141 aaggcagcaa agacatcatc aaaatcaaag gcgtgtgctt ggacgacaaa gccgccgacc
     4201 gcctcgccct gttcgccccc gaagcggtgg tcaacaccat cgacaatttc aaggtcgtgc
     4261 agaagcggca tttgaacctg cccgacgaaa tcgccgaagt gttccgctgt ccgaacacga
     4321 attgcgccgg ccacggcgag ccggtcaaaa gccggtttta tgttaaaaag cacaacgggc
     4381 agacgcggct gaaatgccac tactgcgaaa aaacctacag ccgggattcg gtggcggaag
     4441 cctgacggat tcccttaaac cgagtgggcg gcatttcgtc tgccgcctgt tttgccaatc
     4501 tgaaatggaa tgatgatgca cgcttctgtc caaagccgtt tcgcaccgat actttatgtt
     4561 ttgattttct ttgccggttt tttgaccgcg caaatctggt tcaatcagaa agcctatact
     4621 gaagagctgc ctccgcttct gtccgcattg tccgccgtcg cgctggtgtg gctggcgtgg
     4681 gcgttcgtgt cggcgcgttc aaaggccaag gcggaaaagt tctaccgcga aaaaatgata
     4741 cagaacgaaa gcatacaccc cgtcctgcac gcctctttgc aacacttgga acacaagccg
     4801 caaatactcg ccctgctggt caaaaaccac ggcaaaggga tggcggaaca ggtcaggttc
     4861 aaggcggaag tgctgcccga cgacgaagac gcgcgcacga ttgccgccga gttggcaaaa
     4921 atggatatgt tcgcattggg gacggacgcg gtcgcctcgg gcgaaaccta tggacgcgtg
     4981 ttcgccgata ttttcgagtt gtcggcggct ttggaagggc gcgcgttcaa aggaatgttg
     5041 aaactgacgg cggaatataa aaacatcttc ggcgatgcct gccgttcgga aacggcgttg
     5101 gagttgggcg cactcaatca ggcgttgcag gagatttcaa aaacatcgga aaagtccaaa
     5161 cggatatttt attgaagatg gaaaaatgcc gtctgaaacg gaaggtgttt cagacggcat
     5221 ttttgtcgga tgattaatta ttcggagcgg ttgaagccaa acttcacgcg gctgcggccc
     5281 tgatccggta tattgtccaa atcgcgtccc ggattggcgg cggtgtcgcc tacggaaata
     5341 tcggagatgt tttccaaaat gatggcggac gacaggtgtt cggaggtgcg gtaaaccatt
     5401 gccaagccca cttcttcggc aggagtggaa atcagctcga cggtatccct gcttttgaaa
     5461 ttgttggaga ggtcgacctg catcgttttc ttgcgtttgt agaggctcaa aaccgtgcct
     5521 ttgtccaaac cgtccgcctc gcctttgtcg atggtgatgg tttgaaactg gccggcaatc
     5581 cttgtgcctt caaacacgga aacgatttta gcctgaaccg ggcgggacgg ttcgtgcggc
     5641 atcatgttga agcggtcggt gtcttccggc attttcatca ggtagtcgcc ctgctgtatt
     5701 tcggaaatgg cggtttcgac caccagcggc tgtatcgaag gggtgcgcag cggggtaatc
     5761 aaaggatggg tgcgggtatg gtattcgttg tctttcggcc gttctccagc ctgtttcgag
     5821 cgttgttcga ggacagagtc ggtatagtcg agggagcgca cgatgccgct gaatgcgact
     5881 tcctgcccga ggaatttacc cgtatccgga tcggtgatgt ttttattgat tcggtaggtc
     5941 aggtagcggc ccggctcttt caggcctttg gtgtaaaccc tggtgccttt ggtgtacagc
     6001 agcctgcctt ccgggcccga gagcaggcgc ggcgcggcag cggtttcttt gcgggaaacg
     6061 atttgcggat gccgcataaa gatgcggtag aagttgacat cgatggcggg aataccgtat
     6121 ccggacactt ccttatccgg actcattttg acgacgggga tgccgtctgt ctgttccaag
     6181 ccgaggcgcg gttcgccgtc aacgtggcgc aacaccaata cctggtccgg ataaatcagg
     6241 tcgggattgt ggatttgatc ccggttcgcg tcccacaggc ggccccattg ccacgggctg
     6301 tacaggtatt tgcccgaaat gccccacagg gtgtcgccct gtttgaccgt gtagcgttcc
     6361 ggcgcgttcg ggcgcacctc caaatttgcc gccaaagttt gtgttgagaa tgccatacct
     6421 gccgcgcaga gcagggttat aatacgacgt tgcataaccg ttccccttat ctgataaatt
     6481 tcggtttgtc ttgcttgatt gggttggaaa aagcggcggc agcccctcgg gatgtgccgc
     6541 gtgataaaaa atgttccgca ttttaacatc gaattatccg caccatcacg gtaattatga
     6601 aaaacaggcg gcgtatccgc cgaaggaaag agaaaattat ggctttattg aatatcttgc
     6661 aatatcccga cgagcgtctg cacacggtgg caaagcctgt cgaacaagtc gacgagcgca
     6721 tccggaagct gattgccgat atgtttgaaa cgatgtacga atcgcgcggc atcgggctgg
     6781 cggcgacgca ggtcgatgtg cacgagcgcg tggtcgtgat ggatttgacc gaagaccgca
     6841 gcgaaccgcg cgtgttcatc aaccccgtca tcgttgaaaa agacggcgaa accacttacg
     6901 aagagggctg cctgtccgtg ccgggcattt acgacaccgt aacccgcgcc gaacgcgtca
     6961 aggtcgaggc tttgaacgaa aaaggcgaaa agttcacgct ggaggcggac ggcttgttgg
     7021 cgatttgcgt gcagcacgag ttggaccacc tgatgggcat cgtgtttgtc gaacgccttt
     7081 cccaactcaa gcaggggcgg attaagacca agctgaaaaa acgtcagaaa catacgattt
     7141 gacccttttg ccgtgccgtc tgaacgctgc aaagttttca gacggcacgg tcttgtccga
     7201 caattttacg cacgcgcagg aacacgctat gaaagtcatc ttcgccggca cgcccgattt
     7261 tgccgccgcc gccttaagag ccgttgccgc cgccggtttt gaaattccgc tggtgctgac
     7321 ccagcccgac cgtccgaaag ggcgcggtat gcaactgact gccccgcccg tcaaacaagc
     7381 cgcgctggaa ctcggtttgc gcgtcgaaca gcccgaaaag ctgcgcaaca acgccgaagc
     7441 cctgcaaatg ctcaaagagg tcgaggcaga cgtaatggtg gttgccgcct acggtttgat
     7501 tctgccgcag gaagtgttgg atacgccgaa acacggctgc ctcaacatcc acgcttcgct
     7561 gttaccccgt tggcgtggcg cggcgccgat tcaacgcgcg attgaagccg gcgatgccga
     7621 gacaggcgtg tgtattatgc agatggacat cggtttggac accggcgatg tggtcagcga
     7681 acaccgctac gccatccaac cgaccgatac cgccaacgaa gtccacgacg cgctgatgga
     7741 aatcggtgcg gcggcggttg ttgccgattt gcaacagctt caaagcaaag gccgtctgaa
     7801 cgcggtcaaa cagcccgaag aaggtgttac ttacgcgcaa aaattgagca aagaagaggc
     7861 gcgtatcgat tggagcaaaa gcgcggcggt tatcgaacgc aaaatccgcg ccttcaaccc
     7921 cgtgcctgcc gcgtgggttg agtatcaggg caagccgatg aaaatccggc gggcggaagt
     7981 ggtggcgcaa caaggcgcgg caggcgaagt gttgtcctgt tcggcggacg gtttggtcgt
     8041 tgcctgcggc gaaaacgcgc tgaagattac cgaattgcag cctgccggcg gcaggcggat
     8101 gaatatcgcg gcgtttgcag caggacggca tatcgaagca ggggcgaagc tgtaaatccc
     8161 ttcagacggc attccgatcc gcaaacggga atgccgtctg aaaccatcag tcgaagaaag
     8221 cgaatcacat aatatgagta tggcacttgc ccaaaaactt gccgccgaca gcattgcggc
     8281 ggttgccgaa ggacgtaacc ttcaggacgt gttggcgcaa atccgcaccg cgcatcccga
     8341 ccttatggcg caggaaaacg gcgcgttgca ggacatcgcc tacggctgcc agcgttattt
     8401 gggcagtttg aaacatatgc tcgcgcagat gctgaaaaag ccgattggca atccgcagct
     8461 cgaaagcctg cttttggcgg cgttgtacca gctgcattac acgcgcaacg cgccccacgc
     8521 cgtggtcaat gaggcggtgg aaagcatcgc gaaaatcgga cgcgggcagt accgttcgtt
     8581 tgccaacgcg gttttgcgcc gctttttgcg cgaacgcgac aagcttgtgg cttcctgtaa
     8641 aaaagacgat gtagcgaaac acaacctgcc gctgtggtgg gtggcttact tgaaaaacca
     8701 ttatccgaaa cactggcaca acatcgccgc cgcgctgcaa tcccatccgc cgatgacttt
     8761 gcgcgtcaac cgccgacacg gcaatgccga aagctatttg gaaaaactgg tggcggaagg
     8821 tatcgcggct aaggcgttgg acgaatatgc ggttacgttg gaagaagccg tgccggtgaa
     8881 ccgcctgcct ggtttttcag acggcattgt ttcggtacag gacttcggcg cgcagcaggc
     8941 ggcgtatttg ttaaacccga aagacggcga acggattttg gacgcgtgcg ccgcgccggg
     9001 cggcaagacg gggcatatct tggaactggc ggattgccgt gttaccgcct tggacattga
     9061 tgcaggccgt ctgaaacggg tggaagacaa tatcgcgcgt ctgggctttc agacggcatc
     9121 gacggcgtgt gccgatgcac aggacctgtc ggcatggtat gatgggaaac cgtttgatgc
     9181 cgtccttgcc gacgtgccgt gtaccgcctc gggcgtggcg cggcgcaatc ccgacgtgaa
     9241 atggctacgc cgtccgaccg acgcgctcaa aaccgcccgc cagcaggaag ccctgctaga
     9301 tgcattgtgg caggtgctga aaagcggggg aaggatgttg atcgctacct gttccgtgtt
     9361 cgtcgaggaa aacgacggac aattgcaaaa attcctcaac cgccatgccg atgcagaact
     9421 gatcgaatcg cgggtactct taccgaacaa acaccaagat ggcttttatt acgcgcttat
     9481 tcaaaagcag taaatggctg attgtgccgc tgatgctccc cgcctttcag aatgtggcgg
     9541 cggaggggat agatgtgagc cgtgccgaag cgaggataac cgacggcggg cagctttcca
     9601 tcagcagccg cttccaaacc gagctgcccg accagctcca acaggcgttg cgccggggcg
     9661 tgccgctcaa ctttacctta agctggcagc ttcccgcccc gataatcgct tcttatcggt
     9721 ttaaattggg gcaactgatt ggcgatgacg acaatattga ctacaaactg agtttccatc
     9781 cgctgaccaa ccgctaccgc gttaccgtcg gcgcgttttc gacagactac gacaccttgg
     9841 atgcggcatt gcgcgcgacc ggcgcggttg ccaactggaa agtcctgaac aaaggcgcgc
     9901 tgtccggtgc ggaagcaggg gaaaccaagg cggaaatccg cctgacgctg tccacttcaa
     9961 aactgcccaa gccttttcaa atcaatgcat tgacttctca aaactggcat ttggattcgg
    10021 gttggaaacc tctaaacatc atcgggaaca aataatgcgc cgttttctac cgatcgcagc
    10081 catatgcgcc gtcgtcctgt tgtacggact gacggcggca accggcagca ccagttcgct
    10141 ggcggattat ttctggtgga ttgttgcgtt cagcgcaatg ctgctgctgg tgttgtccgc
    10201 cgttttggca cgttatgtca tattgctgtt gaaagacagg cgcgacggcg tattcggttc
    10261 gcagattgcc aaacgccttt ctaggatgtt tacgctggtt gccgtactgc ccggcgtgtt
    10321 tctgttcggc gtttccgcac agttcatcaa cggcacgatt aattcgtggt tcggcaacga
    10381 tacccacgag gcgcttgaac gcagcctcaa tttgagcaag tccgcattga atttggcggc
    10441 agacaacgcc ctcggcaacg ccgtccccgt gcagatagac ctcatcggcg cggcttccct
    10501 gcccggggat atgggcaggg tgctggaaca ttacgccggc agcggttttg cccagcttgc
    10561 cctgtacaat gccgcaagcg gcaaaatcga aaaaagcatc aacccgcaca agctcgatca
    10621 gccgtttcca ggtaaggcgc gttgggaaaa aatccaacgg gcgggttcgg tcagggattt
    10681 ggaaagcata ggcggcgtat tgtacgcgca gggctggctg tcggcgggta cgcacaacgg
    10741 gcgcgattac gccttgtttt tccgtcagcc ggttcccaaa ggcgtggcag aggatgccgt
    10801 cttaatcgaa aaggcaaggg cgaaatatgc tgagttgagt tacagcaaaa aaggtttgca
    10861 gacctttttc ctggcaaccc tgctgattgc ctcgctgctg tcgatttttc ttgcactggt
    10921 catggcactg tatttcgccc gccgtttcgt cgaacccgtc ctatcgcttg ccgagggggc
    10981 gaaggcggtg gcgcaaggcg atttcagcca gacgcgcccc gtgttgcgca acgacgagtt
    11041 cggacgcttg accaagttgt tcaaccacat gaccgagcag ctttccatcg ccaaagaagc
    11101 agacgagcgc aaccgccggc gcgaggaagc cgccaggcat tatcttgaat gcgtgttgga
    11161 ggggctgacc acgggcgtgg tggtgtttga cgaacaaggc tgtctgaaaa ccttcaacaa
    11221 agcggcggaa cagattttgg ggatgccgct tacccccctg tggggcagca gccggcacgg
    11281 ttggcacggc gtttcggcgc agcagtccct gcttgccgaa gtgtttgccg ccatcggcgc
    11341 ggcggcaggt acggacaaac cggtccatgt gaaatatgcc gcgccggacg atgccaaaat
    11401 cctgctgggc aaggcaaccg tcctgcccga agacaacggc aacggcgtgg taatggtgat
    11461 tgacgacatc accgttttga tacacgcgca aaaagaagcc gcgtggggcg aagtggcgaa
    11521 gcggctggca cacgaaatcc gcaatccgct cacgcccatc cagctttccg ccgaacggct
    11581 ggcgtggaaa ttgggcggga agctggatga gcaggatgcg caaatcctga cgcgttcgac
    11641 cgacaccatc gtcaaacagg tggcggcatt gaaggaaatg gtcgaagcat tccgcaatta
    11701 tgcgcgttcc ccttcgctca aattggaaaa tcaggatttg aacgccttaa tcggcgatgt
    11761 gttggcattg tatgaagccg gtccgtgccg gtttgcggcg gagcttgccg gcgaaccgct
    11821 gacggtggcg gcggatacga ccgccatgcg gcaggtgctg cacaatattt tcaaaaatgc
    11881 cgccgaagcg gcggaagaag ccgatgtgcc cgaagtcagg gtaaaatcgg aaacagggca
    11941 ggacggtcgg attgtcctga cggtttgcga caacggcaaa gggttcggca gggaaatgct
    12001 gcacaacgcc ttcgagccgt atgtaacgga caaaccggcg ggaacgggat tgggtctgtc
    12061 tgtggtgaaa aaaatcattg aagaacacgg cggccgcatc agcctgagca atcaggatgc
    12121 gggtggcgcg tgtgtcagaa tcatcttgcc aaaaacggta aaaacttatg cgtagcagcg
    12181 atattttaat tgtagacgac gaaatcggca tccgcgacct gctgtcggaa atcctgcagg
    12241 acgaaggtta ttcggtcgca ttggcggaaa acgccgaaga ggcgcgcaag ctgcgccatc
    12301 aggcgcgccc cgcgatggtg ctgctggata tttggatgcc tgattgcgac ggcatcaccc
    12361 ttttgaagga gtgggcgaaa aacgggcagc tcaatatgcc ggtggtgatg atgagcgggc
    12421 atgccagcat cgataccgcc gtggaagcca ccaaaatcgg cgcgatcgat tttttggaaa
    12481 aaccgatttc cctgcaaaag ctgctgtctg ccgtcgaaaa cgcgttgaag tacggtgcgg
    12541 cgcaaaccga aacggggcct gtattcgaca agctgggcaa cagtgcggcg attcaggaaa
    12601 tgaaccgtga ggtaggggct gcggtgaaat gtgcctctcc cgtacttttg acgggcgagg
    12661 cgggttcgcc gtttgaaacg gtggcacgct atttccataa aaacggtacg ccgtgggtca
    12721 gcccggcaag ggtcgaatat ctgatcgata tgccgatgga actgttgcag aaggcggagg
    12781 gcggcgtttt gtatgtcggc gacatcgccc agtacagccg caacatccaa gccggtattg
    12841 cctttattgt cggaaaggcg gaacaccgcc gcgtcagggt ggtcgcatcg ggcagcaggg
    12901 cggcaggttc agacggcatt gcctgcgagg aaaagctggc ggaactgctg tcggaatcgg
    12961 tcgtccgtat tccgccgctg cgtatgcagc atgaagacat tcccttcctg atacagggga
    13021 ttgcctgcaa tgtggcggaa agccaaaaga ttgcgcctgc ctcattcagt gaagaggcac
    13081 ttgccgcatt gacccgttac gactggccgg gaaatttcga ccaactgcaa agcgtcgttg
    13141 caacgctgtt gttggaggcg gacggacagg aaatcggcgc aggggcggtt tcttcccttt
    13201 tggggcagaa tgtgcctgcc gagggggcgg aagatatggt gggcgggttt aatttcaacc
    13261 tgcccctgcg cgaattgagg gaggaggtgg agcggcgtta tttcgagtac cacatcgccc
    13321 aagaaggtca gaatatgagc caagtggcgc agaaagttgg tttggaacgc acgcaccttt
    13381 accgcaaact caaacagctc ggcatcggcg tttcgcgccg ggcgggggaa aaaaccgaag
    13441 aataggcccg gacggccggt ttaccggctg cgggcttttg ttttcagacg gcatttggtg
    13501 caaatgccgt ctgaaatcgt aaggggacgg attttatgac agaggacgaa cgtttcgcgt
    13561 ggctgcaatt ggcgtttacg ccctatatcg gcgcggaaag tttcctgctg ctgatgcgcc
    13621 gtttcggcag cgcgcaaaat gccctgtccg caccggcgga acaggtggcg gcactgatac
    13681 ggcacaaaca ggcgcttgag gcttggcgca atgcggaaaa acgcgctctg gcgcggcagg
    13741 cggcagaagc ggcattggaa tgggaaatgc gggacggatg ccgcctgatg ctgcttcagg
    13801 atgaagattt tcccgaaatg ctgacgcagg ggctgaccgc gccaccggtt ttgtttttgc
    13861 gcggcaacgt gcaactgctg cacaaacctt ccgccgccat cgtcggcagc cgtcatgcca
    13921 cgccgcaggc gatgcggatt gccaaagatt tcggcaagtc gttgggtggg aaaggcattc
    13981 ccgttgtgtc gggtatggct tcgggcatcg ataccgccgc ccatcagggt gcgttgcagg
    14041 cagaaggcgg caccatcgcc gtgtggggga cgggcataga ccgcatttat ccgccggtca
    14101 acaaaaacct tgcctatgaa atcgccgaaa aaggattgat tgtcagcgag ttccccatcg
    14161 gcacgcggcc gtatgccggc aattttccgc gccgcaaccg cctgattgcc gccctgtcgc
    14221 aagtaacgct ggtggttgaa gccgcgttgg aatccggttc gctgattact gccagattgg
    14281 cggcggagat ggggcgcgaa gtgatggcgg tacccggctc gatagacaat ccacacagta
    14341 aaggctgcca caaactgatt aaagacggcg caaaattggt ggaatgcctg gacgacatcc
    14401 tgaacgaatg cccggggcta ttgcaaaata cgggtgcttc atcatattct ataaataagg
    14461 gaatacctga aaagcgcatc actgccgttc agacggcatc cgaccagctg tctctgcctg
    14521 aaggcaaaat gccgtctgaa aagacggaga accgacccgt cggcggcagt atcttggaca
    14581 ggatgggttt cgacccagtt catcccgacg tgcttgccgg acagttggct atgcctgccg
    14641 cagatttgta tgccgcactg ttggaattgg aattggacgg cagcgttgcc gcaatgcccg
    14701 gcggcagata ccagcgtatc cgaacttgaa cgcactttat attaaggaac acgaatgacc
    14761 gaagtcatcg cctacctcat cgaacatttc caagatttcg atacctgccc gccgcccgaa
    14821 gacttgggta tgctgcttga agaagcgggt ttcgatacga tggaaatcgg caacaccctg
    14881 atgatgatgg aagtattgct caacagctcc gaattttccg ccgaacccgc cgacagcggc
    14941 gcattgcgcg tgtacagcaa agaagaaacc gacaacctgc cgcaggaagt gatggggctg
    15001 atgcagtatc tgattgaaga aaaagccgtc agctgcgaac agcgggaaat catcatccac
    15061 gcgctcatgc acattccggg cgacgaaatt accgtagata ccgccaaagt gctgaccctg
    15121 ctgcttttat gggcaaacaa gagcgagctg cccgtgttgg tcggcgacga gctgatgagc
    15181 gcgcttttac tcgacaacaa acccacgatg aactgaagcg gcttcagacg gcccgcccga
    15241 gtccgtctga aacgtcggca tcaaaaccac catccagaga acgacaaatg gcgaaaaacc
    15301 tattaatcgt cgaatccccg tccaaagcca aaaccctgaa aaaatatttg ggcggcgatt
    15361 ttgaaatcct tgcatcctac ggacacgtcc gcgacctcgt ccccaaaagc ggcgcggtcg
    15421 atcccgacaa cggctttgcg atgaaatacc aactcatcag ccgcaacggc aaacacgtcg
    15481 atgccatcgt cgccggtgcc aaagaagctg aaaacatcta cctcgccacc gacccggata
    15541 gggaaggcga agccatttcc tggcatcttt tggaaatcct caaatccaaa cgcggcttga
    15601 aaaacatcaa gccgcagcgt gtcgtgttcc acgaaatcac caaaaacgcc gtgctcgatg
    15661 ccgttgccca tccgcgcgaa atcgaaatgg acttggtcga tgcgcaacaa gcccgtcgcg
    15721 ctttggacta tttggtcggt ttcaaccttt cgccattgtt gtggaaaaaa atccgtcgcg
    15781 gtttgagcgc gggccgtgta caaagccccg cactgcgttt gatttgcgaa cgcgaaaacg
    15841 aaatccgcgc gtttgaagcg caggaatatt ggacggtaca tctagacagc cacaaaggcc
    15901 gcagcaagtt caccgccaaa ctcgcccaat acaacggcgc gaaactcgaa caattcgacc
    15961 tgccgaacga agccgctcaa gccgatgtgt tgaaagaact cgaaggcaaa gaggccgtcg
    16021 ttaccgccat cgaaaagaaa aagcgcagcc gcaaccccgc cgcgccgttt accacatcca
    16081 ccatgcagca ggatgctgtg cgcaaactcg gcttcaccac cgaccgcacc atgcgtaccg
    16141 cccagcagct ttacgaaggt attgacgtag ggcagggtgc catcggtctg attacctata
    16201 tgcgtaccga cagcgtgaac ttggcggatg aagccttaac cgaaatccgc cattacattg
    16261 aaaacaaaat cggcaaagaa tatctgccga gtgccgccaa acaatacaaa accaaatcca
    16321 aaaacgccca agaagcgcac gaagccatcc gcccgacttc cgtgtaccgc acgcccgaaa
    16381 gcgtcaaacc cttcctgagc gccgaccagt tcaaactcta tcaaatgatt tggcagcgta
    16441 ccgtcgcctg tcagatgacg cccgccaaat tcgaccaaac caccgtcgat attaccgtcg
    16501 gcaaaggcgt attccgcgta accggacaag tgcaaacctt cgcaggcttc ctcagcgttt
    16561 acgaagaaag cagcgacgat gaagaaggcg aagacagcaa aaaactgccc gaaatgagcg
    16621 aaggcgacaa attgcccgtg gacaaactct acggcgaaca acactttacc actccgccgc
    16681 cacgctacaa cgaagccacg ctggttaaag ccctcgaaga atacggcatc ggccgcccct
    16741 cgacctacgc cagcatcatc tccacgctca aagaccgcga atacgttacc cttgagcaaa
    16801 aacgctttat gcccaccgac acaggcgaca tcgtcaataa attcctgacc gaacacttcg
    16861 cccaatacgt cgattaccac ttcactgcca aactcgaaga ccagcttgac gaaattgccg
    16921 acggcaaacg ccaatggatt cccttgatgg acaaattctg gaaaccgttc atcaaacaag
    16981 tggaagaaaa agaaggcatc gaacgcgcca aatttaccac gcaggaactt gatgaaacct
    17041 gcccgaaatg cggcgaacac aaactgcaaa tcaaattcgg caaaatgggt cgttttgttg
    17101 cgtgtgccgg ttatcccgag tgcagctaca cgcgcaatgt caacgaaacc gccgaagaag
    17161 ctgccgaacg catcgccaaa gccgaagccg aacaggccga actcgacgga cgcgagtgcc
    17221 cgaaatgtgg cggtcgccta gtgtacaaat acagccgcac cggcagcaaa ttcatcggct
    17281 gcgtcaacta tccgaaatgc aaacacgtcg agccgctgga aaaaccgaaa gataccggcg
    17341 tccagtgtcc gcaatgcaaa aaaggcaacc tcgtcgagcg caaatcccgc tacggcaaac
    17401 tgttttacag ttgcagcacc tatcccgact gcaactacgc cacttggaac ccgcccgttg
    17461 ccgaagaatg cctgaactgc cattggccgg tcttgaccat caaaaccact aaacgctggg
    17521 gtgtagaaaa agtctgccca caaaaagaat gcggctggaa agaacagatt gaaccgcccg
    17581 cgccgaagga gtaagattag gttggtttga aagagaaaag gtcgtctgaa aaattttcag
    17641 acgacctttg cttttctgtg attggtttat ttgaatccgc gtgttgtttt aaagtccgat
    17701 aaaatccggt tcatttcagg cgcaaacaag gcgatgtaat cgtaagatag accgcgactg
    17761 gcactgggat ggggaaagca gacgacttcg caatcttcaa acgattggaa tttgacattg
    17821 aaacgtgtac cgtcaaattc tttttgcacc gtctccagcg gtttggtctg cttaccgacc
    17881 aactgctcga agcgtggcag tacattttgg ttgttcagaa aatccgccaa cctgctgccc
    17941 atgaagagga tgactttcgg acgcagtttt tcgatgtggt agagaaaatt atcgatgtgc
    18001 tcgggttgtg tgaacttgtc gggattgtcg atagtgttgc cctgtgtagc agcccagttg
    18061 gtttgaacca gggatttttc aaatgcaccg cccaatccat tttcgtctaa ggggtgtccc
    18121 cacatttcaa accaattttt tatcgtattg tcgtaacgcc acttttttgc ctgctctccg
    18181 aaatagaggg atttgtttgc aaatgtatgg tcgattttgt tttcagggag tttgtattca
    18241 cctgctacat aagcagcctc atcggcttta ctccaacccc attcatagcc acaaatcatt
    18301 aagccatgtt tgtcgttgta gcctttgaac aggctgttgc tcaaattcaa atccttcatc
    18361 atgaactctt ccttttaaaa tttaagagcg attgacttca atgtttttag atggggtgga
    18421 aaaatccttg tgtaggcaac ataaattcaa taaatttctt gataattcga aacctactaa
    18481 tagcgcacct ataaaagctt tttcattacg ttcagcatga cggtcacgtc gttcatattt
    18541 tttacgcttg ctgttccctg ttattacagc taagccaagt gatatggcga gaattgccca
    18601 aacaatagta cttaataaca attttcccca tactatcaat aaggaaagaa aaaacctttt
    18661 agtattagat cgataggtta taatccatgc ccatgaaaat gcaataagag ttatacataa
    18721 gatacaagct gacaggattt ctttattttt aattaaataa cttagagcga tgaggatgac
    18781 aggtgtcaag aaaataatag ttacatcccg atagctataa aagaaaactg ccctattttg
    18841 aatgtggaga tgtgcacaga atccaatata gctaaggata atagttaata taataaaaaa
    18901 agaccaccaa gggtgaagag ataggaattc catgttttcc ctttttttgt aaaaaggaaa
    18961 aaatcttatc taatagttaa ttgcctaacc agccagaaga agtttaaaat ctatcccaat
    19021 aattcaacca tctatacaga aagttcagct tatggaaacc cacgaaaaaa tccgcctgat
    19081 gcgcgaattg aataaatggt cccaggagga tatggcggaa aagctggcga tgtcggcagg
    19141 cgggtatgcc aaaatcgaac ggggcgaaac gcagttaaat atcccgcgtt tggagcagtt
    19201 ggctcagatt ttcaaaatcg atatgtggga cttgctcaaa tcgggcggtg gtgggatggt
    19261 gtttcagatt aatgaaggtg atagtggtgg cgatattgcg ttgtatgcgt cgggtgatgt
    19321 ttcgatgaaa atagaatttt taaaaatgga gttgaaacac tgcaaagaaa tgttggaaca
    19381 aaaagacaaa gaaatcgagc tgctccgcaa gctgaccgaa accgtttaaa cagatatgcc
    19441 gtctgaaaaa agttttcaga cggcatattc tttgacaggt cttgtataat accgtttgaa
    19501 cttacaggtt tttgattatg gcggcaggca aacataccaa acacagcaac cgggtacgca
    19561 ttatcggcgg gcaatgccgg ggcaggaaat tgagtttcac atccgccgac ggactgcgtc
    19621 cgacacccga cagcgtgcgt gaaaagctgt ttaactggct gggacaggat ttgacgggta
    19681 aaacggtttt ggatctcttc ggaggcagcg gcgcactcgg tatagaagcc gcttcgcgca
    19741 acgccaaacg cgtgctgatt tcggataaca accgccaaac cgtgcagacc ttgcagaaaa
    19801 acagtcgcga actgggtttg gggcaggtgc aaatcgtctt ttcagacggc atcgcatatt
    19861 tgaagaccgt atccgaacag tttgatgttg tctttctcga cccgccgttt gcatggcagg
    19921 actggcaaat cctgttcgat gccttgaagc cgtgcctgaa cccccgggca ttcgtctatc
    19981 tcgaggcggg tacgctgccg aatattcccg attggctgac ggaatataga gaagggaaat
    20041 cggggcagag tacatttgaa ttaagggttt tccaagtggc tgaataatat gcgctttgat
    20101 aatcatttcc gagttgtaaa cattcgtttg caaccgtccg gttcaaaaaa accttgtgct
    20161 ataatccgcg cccgcccggt tttgataatt tagtggaaaa ggaaaagaaa tgtcgctttt
    20221 tattaccgac gagtgcatca actgcgacgt atgcgaaccc gaatgcccca atgatgccat
    20281 ttcccaaggc gaggaaattt acgaaatcaa ccccaacctc tgcacgcagt gcgtcggaca
    20341 ctacgatgag ccgcagtgcc agcaggtttg cccggtggac tgcatcctga ttgacgaaga
    20401 acatcccgaa acccatgacg agttgatggc gaaatacgaa aagattatcc agtttaaata
    20461 aattcttttt aaaacatcaa attatgtctg ttttgaaata aaatcaaaaa aaaacttgac
    20521 ggaaaagcaa gccgctaata aactaacgtt ctcttttgga gggattcccg agcggtcaaa
    20581 gggggcagac tgtaaatctg ttgcgaaagc ttcgaaggtt cgaatccttc tccctccacc
    20641 aaaattctta cttggggcag tagcgagtaa tgcgggtgta gctcaatggt agagcagaag
    20701 ccttccaagc ttacggtgag ggttcgattc ccttcacccg ctccaaacaa ttaggcccat
    20761 gtagctcagg ggtagagcac tcccttggta agggagaggt cggcagttca aatctgccca
    20821 tgggcaccat ctctcgatta ttcatttctt taaggcttag atatatagga tattgccatg
    20881 gctaaggaaa aattcgaacg tagcaaaccg cacgtaaacg ttggcaccat cggtcacgtt
    20941 gaccatggta aaaccaccct gactgccgct ttgactacta ttttggctaa aaaattcggc
    21001 ggtgctgcaa aagcttacga ccaaatcgac aacgcacccg aagaaaaagc acgcggtatt
    21061 accattaaca cctcgcacgt ggaatacgaa accgaaaccc g
//
LOCUS       DAWGRZ010000015        20931 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000015 DAWGRZ010000000
VERSION     DAWGRZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20931
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.015"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..144
                     /locus_tag="WHT44_000402"
     CDS             <1..144
                     /locus_tag="WHT44_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEZ2532633.1"
                     /translation="NVGIGGGALLGHWVTQYSGISCIGVAGMLTAAAGLWVCLNLNRH
                     IRT"
     gene            273..1709
                     /locus_tag="WHT44_000403"
     CDS             273..1709
                     /locus_tag="WHT44_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ2532634.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIIVLYSSCVYLLGELLRSDRENTRRFWLGCGIAASLTVLGFFKYFDFFRPMI
                     AQYAGKGGAIDILMPLGLSYYTFQSLAYLVYCFRAPHAARFSWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRRARSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLGGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RALNIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRDALCRLKYFAPLSWLITFHFVCLSFVVFNTANPD
                     DAGAVFSALFANANGWNAPQQANMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            1720..2703
                     /locus_tag="WHT44_000404"
     CDS             1720..2703
                     /locus_tag="WHT44_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ2532635.1"
                     /translation="MKNFLSLFSSILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYALSDGIKAFLSGETPPTAQDGGSADMPSEAAASEAVPQTGET
                     EWKQDTEAAAVRSGDKVFFVGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLQKHPEISVLAVFLGPNDPWDFPVGKLYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLKGKIILIPTTHTLSGGKDR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            2700..3893
                     /locus_tag="WHT44_000405"
     CDS             2700..3893
                     /locus_tag="WHT44_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ2532636.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNASASPWMKKLQSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNTGDFPLGGILAHTGSGGSMTLTASDG
                     IASKQRVSLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLTIHTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILSYGTNEAFNNNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRS
                     AAIRQ"
     gene            complement(3953..4129)
                     /locus_tag="WHT44_000406"
     CDS             complement(3953..4129)
                     /locus_tag="WHT44_000406"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532637.1"
                     /translation="MPSENGRRQFIIFAFRRHIKRRLCAVCRRFACVLPMPEANAGCV
                     RQTVRGVAMPSEAI"
     gene            4180..5733
                     /locus_tag="WHT44_000407"
     CDS             4180..5733
                     /locus_tag="WHT44_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ2532638.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAISAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPTGETAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQTLLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(5823..7850)
                     /locus_tag="WHT44_000408"
     CDS             complement(5823..7850)
                     /locus_tag="WHT44_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ2532639.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLGLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKQTASPAMHELQRELSEEYGLSVR
                     VDKMFHRDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            8139..10145
                     /locus_tag="WHT44_000409"
     CDS             8139..10145
                     /locus_tag="WHT44_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ2532640.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGSLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            complement(10268..11377)
                     /gene="mltB"
                     /locus_tag="WHT44_000410"
     CDS             complement(10268..11377)
                     /gene="mltB"
                     /locus_tag="WHT44_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ2532641.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRAVEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNVGDVAASVANYMKQHGWRTGGKMLV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(11286..11537)
                     /locus_tag="WHT44_000411"
     CDS             complement(11286..11537)
                     /locus_tag="WHT44_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532642.1"
                     /translation="MPSDGIFGDDIRISGGARGNVRHSAKPPIAADAAFRAAPLCRFP
                     TYPICSEHIYEKEKNTAAGNLFGGFVCLYGDGGTPTPGK"
     gene            complement(11579..13006)
                     /locus_tag="WHT44_000412"
     CDS             complement(11579..13006)
                     /locus_tag="WHT44_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ2532643.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKYGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQVAQGLEMIFKGEGGGLGVTEPETSGAAIAREMQSYYEYIDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIKFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AKHEDTAAFLLNDIRKDILDCRYCG"
     gene            13191..17108
                     /gene="mfd"
                     /locus_tag="WHT44_000413"
     CDS             13191..17108
                     /gene="mfd"
                     /locus_tag="WHT44_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ2532644.1"
                     /translation="MTYPIPKPREKSRWPNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKSDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTETQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLDDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQYLYLSADVFAGRLKNYGQVLP
                     DVSGKEYTLPDLAVNRQADEPLQALKDFQTAFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQPTPSPVGEGWGEGKAVA
                     AQTEFPASATNPLPSPLPQEREQSAAAVSDDLKTKSSLHPVANNLHGQIRQQPTPSPV
                     GEGWGEGKAVAAQTEFSAAATNPLPSPLPQEREQSAAVVSDSLKAAAVSTESSLPPGK
                     SNLHGQIQQQPAPSPVGEGWGEGKAVAAQSAIAVITESDLYQYVARSRIHNRRKKHAA
                     VSDGLLRDLAEINIGDPVVHEEHGIGRYMGLVTMDLGGETNEMMLLEYAGEAQLYVPV
                     SQLHLISRYSGQAHENIALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAQRAAQSGH
                     KFEINELDYQAFADGFGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVAL
                     RAAFVAVMGGKQVAVLAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKAALE
                     GMADGTVDIVIGTHKLVQDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRANVDILTMT
                     ATPIPRTLSMALEGLRDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVF
                     FLHNEVDTIENMRERLETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTII
                     ETGIDIPNANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLD
                     AIAAADELGAGFTLAMQDLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQAVRDLKKG
                     RQPDLDAPLGITTEIKLHSPALLPEDYCPDIHERLVLYKRLAVCETVQQINTIHEELV
                     DRFGLPEQPVKTLIESHHLRLMAKELGIDAIDAAGEAVTVTFGKNNNVDPTEIILLIQ
                     NDKKYRLAGADKLRFTAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            17257..17640
                     /locus_tag="WHT44_000414"
     CDS             17257..17640
                     /locus_tag="WHT44_000414"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ2532645.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            17662..18504
                     /gene="kdsA"
                     /locus_tag="WHT44_000415"
     CDS             17662..18504
                     /gene="kdsA"
                     /locus_tag="WHT44_000415"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ2532646.1"
                     /translation="MDIKINDITLGNNSPFVLFGGINVLESLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVVAMAKTGNVVNIKKPQFLSPSQMKNIVEKFHEAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            18519..18959
                     /locus_tag="WHT44_000416"
     CDS             18519..18959
                     /locus_tag="WHT44_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ2532647.1"
                     /translation="MMQTFRKISRYVATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANRFLITPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSIVFMATALLSAVLSWRLSGKDAV"
     gene            19007..20293
                     /gene="eno"
                     /locus_tag="WHT44_000417"
     CDS             19007..20293
                     /gene="eno"
                     /locus_tag="WHT44_000417"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ2532648.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGRVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPSKAAFYQLGK"
     gene            20307..20585
                     /gene="ftsB"
                     /locus_tag="WHT44_000418"
     CDS             20307..20585
                     /gene="ftsB"
                     /locus_tag="WHT44_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ2532649.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(20626..20916)
                     /locus_tag="WHT44_000419"
     CDS             complement(20626..20916)
                     /locus_tag="WHT44_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ2532650.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
BASE COUNT         5001 a         6334 c         5214 g         4382 t
ORIGIN      
        1 aacgtcggta tcggcggcgg cgcgctgctg gggcattggg ttacgcaata ctcgggcatt
       61 tcctgcatcg gcgttgcggg tatgctgacg gcggcggcag gtttgtgggt ctgcctgaac
      121 ctgaaccgcc atatccgaac ctgaccgcac gatgccgtct gaagcccctc ccgcccttca
      181 gacggcattt tgattgaaga aatatccacc cctgcttaaa ataacgggct ttgccgtgtt
      241 ttaacgccta ttttttgttc caaggatggt ttatgccgct gctgtctgtc gagttcgcac
      301 tgttctttct cgccttcctg ccgatttact ggggcttggc gaaatacccg tccgtccaaa
      361 acctgctgct tttggctgcc ggtatgggct ggctctacca tatcggccct gtatttgcgg
      421 caatcatcgt cctttattcc tcctgcgtgt accttttggg cgaactgctc cgttccgatc
      481 gcgaaaatac gcgccgtttc tggctggggt gcggcattgc cgcctcgctg accgtcttgg
      541 gctttttcaa atatttcgac tttttccgcc cgatgattgc ccaatatgcc ggaaaaggcg
      601 gcgcaatcga catcctgatg ccgctggggc tttcgtatta caccttccag tcgctcgcct
      661 atctggttta ctgcttccgc gccccgcacg ccgcgcgttt cagctggcac gagctgctgc
      721 tgcacctgag ttttttcccc accgttacct ccggcccgat tatccgcgcc gccgcattca
      781 aaagcgcaga cggcgagcag gcaggcgcat tggcgcaaat ccgtacccgc cgagcgcgtt
      841 cgcccgtccg ccccgcactc gccgtttccc tgattctgct gggtattgcc aaaaaatggt
      901 ggctggcggg gatgctggcg gaaaactggg tgtcgcccgt atttgaaaat cccgcccaat
      961 tcgacggctg gggcgtattg ggcggcgtgt acggctatac cttccaactc tttttagact
     1021 tttccggata ttccgatttg gttatcggca tggcgatgct gctgggcttt aggctgccca
     1081 aaaatttctc cgcaccgctt cgtgctttaa acatccgcgc attttgggac aaatggcaca
     1141 tcagcctttc cacctggata cgcgactaca tctacatccc cttgggcggc agcaaaaaag
     1201 gctttttacg gacacagctc aacctgatgg cggcaatggt gctctcaggc atctggcacg
     1261 gctacggctg gaacttcctc atttggggcg cgctgcacgg cacggcactg gtgctgctca
     1321 acacgggcga ccgctatttc ggacgcgacg cgctatgccg tctgaaatac ttcgcgccgc
     1381 tctcatggct cattaccttc catttcgtct gccttagctt tgtcgtcttc aataccgcaa
     1441 atcccgacga tgcaggcgca gttttcagtg ccctctttgc caatgccaac ggctggaatg
     1501 cgccgcaaca ggcaaacatg ctgttgcttg cctcgtttgc atccgtgatg ctgctctacc
     1561 cttacctgca acgcgctttc gacggcgcgg tcaaaggttt ggaaaaaatc ccgatgtggc
     1621 tgtggtttat ccccgtttcc gccgtcctgc tgctgattat cgtcctcgcc ccctcgggga
     1681 tacccggctt tatttatgcc aatttttaag ggtttggaca tgaaaaactt tctttccctt
     1741 ttctcctcca tactgatgtc tgccctgatt gccgtgtggt tcagccaaaa ccccatcaac
     1801 gcctactggc agcagaccta ccaccgcaac agcccgctcg aaccgcttgc cgcctacgga
     1861 tggtggcgga gcggtgcggc gttgcaagaa aacgcctacg ccctttcaga cggcatcaaa
     1921 gccttcctgt ccggcgaaac gccgccgacg gctcaagacg gcggttcggc agatatgccg
     1981 tctgaagccg ccgcatccga agccgtccct caaaccggtg aaacagaatg gaaacaagac
     2041 accgaagccg ccgccgtccg cagcggcgac aaagtctttt ttgtcggcga ctcgctgatg
     2101 cagggcgttg cccccttcgt gcaaaaaagc ctgaaacagc aatacggcat cgaatccgtc
     2161 aacctcagca aacaaagcac ggggctgtcc tacccctcat tcttcgactg gccgaaaacg
     2221 attgaagaaa ccctgcaaaa acatcccgaa atcagcgtac tcgccgtctt cctcggaccg
     2281 aacgacccgt gggatttccc cgtcggcaaa ctctatctca aattcgcttc cgacgaatgg
     2341 gcgcaagaat acctgaaacg tgtcgaccgc atccttgaag ccgcacacac gcaccgcgtc
     2401 caagtcgtct ggctcggcat cccctacatg aaaaaagcca agctcgacgg acagatgcgc
     2461 tacctagaca aactgctttc ggaacatttg aaaggcaaaa tcatcctgat tcccaccacg
     2521 cacaccctga gcggcgggaa agaccgctac accgactccg tcaacgtcaa cggcaaaccc
     2581 gtccgctacc gcagcaagga cggcatacac tttaccgccg aaggacaaaa actgctggcg
     2641 gcaaaaataa tggaaaaaat cgtttttgaa ccaagtacgc aaccatcaag tacacagcca
     2701 tgaaccccaa acacctcatc gcattttccg ccctattcgc cgccacgcag gcagaagccc
     2761 tacctgtcgc ctccgtcagc ctcgacaccg ttaccgtttc cccgtccgcc ccctacaccg
     2821 atacaaacgg gctgctgacc gactacggca acgcctccgc ctcgccttgg atgaaaaaac
     2881 tccaatccgt cgcacaaggc agcggcgaga ccttccgtat cctgcaaatc ggcgactcgc
     2941 ataccgccgg cgacttcttt accgacagcc tgcgcaaacg cctgcaaaaa acttggggcg
     3001 acggcggcat aggctgggtt taccccgcca acgtcaaagg gcagcgcatg gcggccgtcc
     3061 ggcacaacgg taactggcaa agcctcacca gcaggaacaa caccggagac ttcccgctcg
     3121 gcggcatcct cgcccacacc ggcagcggcg gcagcatgac cctgaccgca tcggacggca
     3181 tagcaagcaa gcagcgcgtt tccctgtttg ccaaacccct gcttgccgaa caaaccctga
     3241 ccgtcaacgg caacaccgtc tccgccaacg gcggcggctg gcaggtactg gatacgggcg
     3301 cggcactgcc cctgaccata cacaccgaaa tgccgtggga catcggcttc atcaacatcg
     3361 aaaatcccgc cggcggcatt accgtttccg cgatgggcat caacggcgca caattaaccc
     3421 agtggtcgaa atggcgtgcc gaccgtatga acgacctcgc ccaaaccggc gccgatttgg
     3481 ttatcctttc ctacggcacc aacgaagctt tcaacaacaa catcgacatt gccgacaccg
     3541 aacaaaaatg gctggatacc gtccgccaaa tccgcgacag cctgcctgcc gccggcatcc
     3601 tcatcatcgg cgcacccgaa tccctgaaaa acacgctcgg cgtatgcggc acacgccccg
     3661 tccgcctgac cgaagtccaa cagatgcagc ggcgcgtcgc ccgtcagggg cagacgatgt
     3721 tctggtcttg gcaaaacgcc atgggcggca tatgcagcat gaaaaactgg ctcaaccaag
     3781 gatgggccgc caaagacggc gtacacttct ccgccaaagg ctaccggcgc gcggcggaaa
     3841 tgctcgccga cagcctcgaa gaactcgtcc gctccgctgc aatcaggcaa taatcggaca
     3901 ggaggcggac ggtatttccg caacaggggg atgccgtctg aaacgcatac cttcatattg
     3961 cttcagacgg catagccacc ccgcgcacgg tttgccggac gcaaccggca ttcgcctcag
     4021 gcatcggaag gacgcaggcg aacctccggc atacggcgca aaggcggcgt ttgatatgcc
     4081 gtctgaaggc aaagatgata aactgccgcc ttccgttttc agacggcata ttgttttcaa
     4141 atgagggcgt tctccgtccg caaccataaa ggaagtttca tgaaccggac ttatgccaat
     4201 ttctacgaaa tgctcgccgc cgcctgccgc aaaaacggaa acggcacggc agtgttcgac
     4261 ggcaaggaaa aaaccgccta ccgcgcgctc aagcaggagg ccgaagccgt cgcggcgtat
     4321 ctgcaaaata tcggcgtgaa gttcggcgac acggtcgcgc tggcggtttc caattccaca
     4381 gaatttatta ccgcctattt cgccatctcc gccatcggcg cggtcgccgt accgatgaac
     4441 acatttttga aaaacagcga atacgcgtat atcctgaacg actgcaaggc gcgcttcctg
     4501 ttcgcctcgg ccggcctgtc aaaagaattg gcgggcttga aggcgcaaac gcccgtcgaa
     4561 aaaatcattt ggacggacaa aagccgtccg accggcgaaa cggcggaagg cgatgccttt
     4621 tttgaagacg tgcgccgctt ccccgaaaaa cccgacttgg gccgccaacc ccggataaat
     4681 gatttggcac acatcatcta cacctccggc acgacggggc atcccaaagg cgcgctaatc
     4741 agttacgcca acctgttcgc caacctgaac ggcatcgaac gcatctttaa aatttccaag
     4801 cgcgaccgct ttatcgtttt cctgccgatg ttccacagct tcacgctgac ggctatggtg
     4861 ctgctgccga tttatatggc gtgttcgatt attttggtca aatccgtttt tccgttttcc
     4921 aacgttttga aacagacact gctcaaacgc gcgaccgtgt ttttgggcgt acccgcgatt
     4981 tacaccgcga tgagcaaggc gaaaatccct tggtatttca gatggttcaa ccgcattcgc
     5041 ctgtttatca gcggcggcgc gcctttggcg gaacaaacca tcctcgattt caaagccaag
     5101 ttcccccgcg ccaaattgct ggaaggctac ggactgagcg aagcctctcc cgtcgtcgcc
     5161 gtcaatacgc ccgagaggca aaaagcccgc agcgtcggca tccccctgcc cggtttggaa
     5221 gccaaagccg tcgatgaaga attggtcgaa gtgccgcgcg gcgaagtggg cgaactgatc
     5281 gtcaggggcg gttcggtgat gcggggctac ctcaatatgc ctgccgccac cgatgaaacc
     5341 atcgtcaacg gctggttgaa aacgggcgat ttcgttacca tagacgaaga cggctttatc
     5401 tttatcgtcg accgcaaaaa agatttgatt atttccaaag gtcaaaatgt ctatccgcgc
     5461 gagattgaag aagaaatcta caaactcgat gccgtcgaag ccgccgccgt catcggcgtg
     5521 aaagaccgtt atgccgacga ggaaatcgtc gccttcgtcc aattgaagga aggtatggat
     5581 ttgggcgaga acgaaatccg ccgccacctg cgtaccgtgc tggcaaattt caaaatcccc
     5641 aaacaaatcc actttaaaga cgggctgccg cgcaacgcta cgggcaaggt attgaaacgg
     5701 gtgttgaagg agcagtttga cggaaacaaa tgaacgccgt gccgtccgaa gccccgtccg
     5761 gcaaaaaaat gcggtgaatc tgattcaccg catttttttg acaaacaccg gagggagggc
     5821 atttactctg ccaattccac ctgctcgtgc gccatcagtt cctgaccgac atcatccaac
     5881 aacatcaaat aacgttcgta gtttttcaaa atgtcggcaa tcagctcgtc cttgttcata
     5941 tactgcacat cgtacccgac gcgcccgtcg aaaaaataag cgtagggttt gtaagttgtc
     6001 tgatgccgga tatgcggcag cttgccgtcg ttaatcaact ggtcggatac atcctgcccg
     6061 acagacttaa tcccgtacat aaaatcgcgc atcgtctctt tccgaatgac gaactcgatt
     6121 gcgggctcgt cccgatgaaa cattttatcg acccggacgc tcaagccgta ttcttccgaa
     6181 agctcccgtt gcaactcgtg catagcgggc gatgcagtct gtttgaggaa ttttaaaata
     6241 tcctgctcct gcgtctggct cattatctgc accagccgtt ctttccactt gccgcccgtc
     6301 caaaatacac tggtagggtt aacccgggtc tcaaaatatt tcttatccgc actcaagcct
     6361 ttccacaggc tgaaacacat tatcagcatc agcagggcaa acggcaggga aacaatcagg
     6421 gtcatagact gcaggttgcc gagtccgccc gagcgcatca gcaaaacggc aacggcagac
     6481 atcagcacgc cccacataac cgcctgccac cgtggcgcgc tcaagccttt gtcccgagag
     6541 gtaatattgt tcaggacata aatcccggaa tcggcagaag ttacaaaaaa cagagaaatg
     6601 accagcaggc tgacgatgct cgtcaattcg ggcaggggga ggtaattaaa gaatttaaaa
     6661 agcagcgttt ccggagagga ggtcatcttt tcgagcattc cccccgcaac cccgtcattc
     6721 agccaaatcg ccgtattgcc gaagacggta aaccacaaaa cgccgaacag gccggggatg
     6781 agcaaaaccc cgaagacaaa ctcgcggatg gtgcgcccct ttgaaatgcg cgcgataaac
     6841 aaacccacaa acggcgccca agaacaccac cacgcccaat aaagcaccgt ccaagattca
     6901 aaccacggct tgtgttcccg ttcgtacgca taagttttaa aactgaggcg caccagattt
     6961 ccgaggtagt tccctatgtt gtcgccgaat gccgacaaca ggtaaacagt gggtcccgcc
     7021 gccaaaacaa aaaacagcag caaaaacgca aggcccaggt tcaactcgct caacaccttc
     7081 acgcccttcc ccacgccgga tattgccgaa acgacggcga gggacatgac ggcggcgata
     7141 atcaaaacct gcacgctgaa gctgttttcg gcaatccagc ccatttcctg caatccggcg
     7201 cccagttgcg aagccccgaa ccccaatgtg gtgatgatgc cgaaaaaagt agcaagcaac
     7261 gccataatat caatggcatc gccgaacctt ccggaaattt tttctttcaa cagggggtaa
     7321 aaacaagaac gcagggcaag cggcagcttg tagcggaaac cgaaataagc caaagccaat
     7381 gcaatcgtac cgtacaccga ccaagcgtga acgccccaat ggaacaccgt gtgcagcaat
     7441 gcctgctgct gcctgtgttc cggcgtgccg gccgtaatgt ccgaaaaata atgcatcaac
     7501 ggctctgcca cgccgaaaaa catcagaccc acgcccatcc cggccgcaaa cagcatcgcc
     7561 agccacgaca ggaagccgaa ttccggcaca tcttcatccc gtccgagcct gatgtttccc
     7621 aaactgctga ccgagagtat cagcaggaaa cccagaaaaa tggaaaacgt taaaacataa
     7681 aaccagctga actcggtaaa aatgacttct tttgcccgat cgagccacat ctgcacctga
     7741 tccggcacgg ttaaaaccaa taccaccaaa acacacacaa aaaacaaagt cgtcaaaata
     7801 accatcggat taaatgacgt tcggcgttct ataaattcag acagggacaa accttctcac
     7861 tcctttgtta aaaacagaca aacccggtca tcgggcaaag cggtcaaaac ctgccggtaa
     7921 aataccggct tccggatgac gaaatgcaca aaccggccga aattgttata atcgaaaaaa
     7981 ttaaaaatca atacggatta ttctatcaga ttgatttatc gatatttatt atatcattaa
     8041 tatagtttga tttcaaaccg gcggaaaatg cgccgcccac cgcaggcgcg ccctccccgc
     8101 tatcggggcg tttaacgccg gattgccgat gcggtacaat ggctgatatg aagaaaatta
     8161 cccctcaaaa cctgcgcccc ctgctttcgg aaagcttggg acataccgat tttgtcaacg
     8221 tcctcaacgc actgattaaa tttttgcgcc gtggcggcaa aaaatgtgcg ggggaacgtt
     8281 tcgacctgat tatcgacaca ttcaaacaag acagggaatt actgtcccgc ttcagccggt
     8341 gtttttacat ttggctcgcg caaatacaca tttatccggc actcatcaaa ctcggcatct
     8401 tctcgcgcca cagctttgcc cgggaaatgg gcatacgcat ctacgaacgc ttcagcccgt
     8461 catataaaga ttttgccaac ttgggcgaag tcttccttta tcttttccat tccgaaaacg
     8521 acgacaaatg gctgcaaacg ctcaatatcc gccaatggct ggttttatac gaactcatcc
     8581 ggagccacgc cgagccgtcc aaattgcaga cggcgggcat ccgccttgcc gatgcgcgtt
     8641 tgcgcgccat cgaaatgctg tctgtctgga cggcatccga agccatcgaa cccgacctca
     8701 tccgcatcgc cccgcgcctg ctggaagccg attcttcctt cgtcgccctc caacgcgaaa
     8761 ccgccaaact ggtcgaacac taccgcaacg gcaccacgcc ttacgacacc gcccacctcg
     8821 aagtgatgtt cgaccaatgt ttcagccaga ttgactattt gcgccgcaaa gggacgggcg
     8881 ccggctccgg ttcgtcggtc aaagtcgccc acctgctcga acggctccgg cagaccgtag
     8941 accgtctgaa gctgctcacc gacatccaaa ccggcgccgg caacagcaac cgcctgacca
     9001 tcgcgctgat gaactccctc atctacgcgg cggtcgaaca atacagcacc cgccacctgc
     9061 gccgcagcag catccgtatg ctcgcccgca gcattaccga aaacaaaagc caccacggcg
     9121 aacactacat cacccgcaac cgcaaagaat atttcaaaat gttctactcg gcggcaggcg
     9181 gcggcatcat catcgcccta atggcgctgc tcaaaatccg catcggctca ctcggcctca
     9241 gccccttcct cacttccttg tcggctgggt tcaactacgg catcggcttt atgatcatcc
     9301 atatgctgca ctgcaccgtc gccaccaagc agcccgcgat gactgccgcc agctttgccg
     9361 aacaggtcga tctcaacgaa ggcggcaaag cggtggacaa caaactcgcc aagctcctca
     9421 tcgacgtatg ccgctcccaa agtgtcgccg tcttcggcaa cgtttccatc gccatccttt
     9481 tggcgtgcgc catatcgttc ggctatgccc atctgtaccg gctgcccata ctcgatgccc
     9541 acaccgccgc ctaccagttc aaatccatag acatcatcgc ttacccgacg ctgtggtatg
     9601 ccgccattgc aggtctgtgg ctgttctgct ccggcatcat cgcaggtttt ttcgacaacc
     9661 gcgccgacta cctcaacctg cgccaacgcc tgcccttcaa ccccttgctg cgtaaaatca
     9721 tgcgccccgg gccccgccgc gtcctcgccg cctacatcca caaacactac ggctcgctgg
     9781 tcggcaactt catcttcggg atgctcttgg gtatgaccgg ctatttcgga cacctcctcg
     9841 ggctgccgct ggacatccgc cacgtcgcct tttcctccgc caacctcggc tatgccgccg
     9901 tcagcggcaa cgtcggtttg ggcacgttcg tactcggcat tttcagcgtc ctcgccatcg
     9961 gcctggtcaa cctctgcgtc agcttcagcc tcgccctctt cgtcgccctg cgctcgcgcg
    10021 gcacgaaaat cggcagcatc cgcaatctga ttaaaagttt ttggaatcag attaaaagca
    10081 atccctgcat acttttcctc ccgcccgcca aagaacaggg acatcctcct tcggacaagc
    10141 cttgaccggc aatgccgtct gaagcgggat tcgccccgaa taccgccctg atgcgggaaa
    10201 tccccataaa aggatgcaaa aatgccgtcc gaaccgaaac gtggttcaga cggcatttta
    10261 aaaaacatta caatcccggg ccgccaagcg aattggcaat gtccctgacc gccgttacat
    10321 acatccggct gtggttgtac tgccataccg tataaaaatt gttcaagccc aaataatatt
    10381 caaacacgcc cggtgcggtt tccagtttga acaaaaccgc cttttcatca tctgcaagct
    10441 cttcgccggg gatgatgccg tacgccttca aatccgccac cgtccgcgtc agggcggttt
    10501 tttcgccaat gattgcctga acatccgcac ccggcgccaa tgttgcagac accagcattt
    10561 tcccgcccgt gcgccaaccg tgctgcttca tataattggc aaccgatgcc gcgacatcgc
    10621 cgacgttgcc ccatatgtcc cgatgtccgt ccccgtcata atccaccgcc catttccggt
    10681 agctcgaagg cataaattgc ggcatcccca ttgcgcccgc atagctgcct ttaaaggcga
    10741 aaacatcgcc gccttcttct tttgccagct ttaaaagctc gaccaattct ttttggaaaa
    10801 acccggcgcg gcgggggtaa tcaaagccta aggtcgccaa tgcgtccgcc acacggaaac
    10861 tgcccgtatt tttgccgtaa ttcgtttcaa tcccgataac cgccacgata agttcggcag
    10921 gcacgccgta tttttgcgcc acatcatcga taagcgcgcg gttttccgca taaaaccggc
    10981 gcgcgccgcg aaatttcgcc ttgcccgaat ttcccgtgcg gaacacatac cacggacgcg
    11041 atgtggaggg gcggtgcata atcttgacga tgtccgcctt gtaagccgct ttgtcaaaaa
    11101 aatcctgcca ttccgcccgg gaaaaatccc ctttcccgac ttcatcgtcc acaaaacggc
    11161 ggacatttgc attggcggca aacccgctgt cggataccgg tacggctgcc gcgtcaaata
    11221 cggctgccgc gtcaaacgcg gggcggcttt cttttttcat ttcaaccgcg cggggggctt
    11281 gggcttcatt tgcccggggt gggcgtgcct ccatcgccgt acaggcagac aaagccgcca
    11341 aacaaattgc cagcggcagt atttttctct ttttcataaa tatgttccga acaaataggg
    11401 taagtgggaa agcggcacaa gggggcggcg cgaaatgcgg catccgccgc aatcggcggc
    11461 tttgcggaat gccgcacgtt gcctcttgca ccgcccgaaa tccgtatgtc gtcgccgaaa
    11521 atgccgtctg aaggcacttc ccctttcaga cggcattgcc tgccgccgta tttgcccgct
    11581 acccgcaata tcggcagtcc aatatatctt tgcggatgtc gttcagcagg aaggctgcgg
    11641 tgtcttcgtg tttcgcctgt acgaagacaa agagtcgggc gatgattttg ccgataaaga
    11701 ctttttgcag ggcgcaggca tcggtcaggg tgtgttcctg caggaagctg cggatgtctt
    11761 caggcaaagt gtagtcgcgg gcgacggcgg caaagcgggc atcggtttgc aggcggtcga
    11821 gcagggtttg gtttttgtcc aacttcatgc ctgcttcttt ttcttcggcg gtggcgcgga
    11881 caaactggtc gtaaatttcg gcataaagca tatcgttcgg gccttcaaaa atcgtgaagg
    11941 ggcggatgtc gatagcgata ttgccggcgg tgtgtccgcg ttcaaaaccc ttcgcaccca
    12001 agagtttttg caacatttgc gcggcggcgt aagtgtattc cgtggcgagg gttttgacga
    12061 tgttcgcctc catcagctga tgggcgacgg gggcaacagg cgaaacggaa tggcagacgt
    12121 agcggtaaag aatctcggaa acctgatggc ggcgccggat ttcgcggcgt tcgtaatcga
    12181 cgaatttgat gtcgttgcgg acgtatcgtt ccagattttc aaggatgtat tccataatgc
    12241 cgtgcgtcat gccgatcagt tgcaggcggc tgcggataaa gatgttttgg aacgcgcgca
    12301 aaccggcagc gtcgctctgg gagagtttca tcacggcggt tgcaggcatt tcggcatcga
    12361 tgcggttgac ggcgtaacgg acggcgcgca agccttcgga tgcgagggtt tcgcagcgga
    12421 tgtatgtttt ggggacgagc agcaggtcga tgactttggc gagtttgccg tttttgcgct
    12481 ctttggcggc aacgaggagg aagtcgcttt gcgagttgcc ctgccagtat ttcgcggcgt
    12541 tgacgtaaat ggtttgtccg tcgatatatt cgtagtagga ctgcatttcg cgtgcaatcg
    12601 ccgcgccgga ggtttcgggt tcggtaacac ccaaaccgcc gccctcgcct ttgaaaatca
    12661 tctccaaacc ttgcgcgact tgcgcttcat cgccgaactc ttgcagtggc tgcaacacca
    12721 gcgcgccttc gatgccggta cgcagcgtaa cgggcacgcc gtaatgcccc gcaatccgca
    12781 ggacttcttg gatttcaaac tggctgccct tgcgcccgcc gtattttttg tcgaggaagg
    12841 gcaacagcaa acccgcctgc ttcaaggcaa gccatttgtc ttcgggcagg tatcgcatca
    12901 ggtcgatacc gtctgaaaaa atgcggcgga atgcggattc gatgtgcttt aaaaaagcag
    12961 ccgtgtccat agttgacggc tgcgcgctcg gttcggtgtg tatcatcggc ttcctctgtc
    13021 ggttcccatt aatcggcggc cggtcaaacc gcctgccaca gtttagagtt gattttctaa
    13081 actttaccac aaagtgcgcc gggcaacaat ccgccgacct ttcagacggc atcgcgtccc
    13141 ctcccgtgct aaaatgaccg tttgcatcac tgtccgccga ttgccgcact atgacctacc
    13201 ccatccccaa accccgtgaa aaatcccgtt ggcccaatct ttcgcaaggc tcgctgccct
    13261 tggctttggc gcgttatctg ccgcacaagc ggctcaaggt cgtgctgacc caagatgcgg
    13321 aacaggcgtt gcgccttcag acggcatggc ggtttttccg tccgcacgac acggcggtgt
    13381 tcctgccgga ctgggaaacg ctgccttacg agcgtttttc gccgcatcag gatttggtgt
    13441 cggagcggct gtcggcgttg tggcagatta aaagcggcgc ggcggatgtg ttgttcgtgc
    13501 cggttgccac ggcgatgcag aagctgccgc ccgtgccgtt tctggcaggg cgcacgtttt
    13561 ggctgaaaac ggggcagact ttggatatag gccgtctgaa aagtgatttg gtggatgcgg
    13621 gctacaacca tgtttcccac gttgtcgcgg cgggcgaatt tgccgtgcgc ggcggtatag
    13681 tcgatttgtt cccgatgggc agcgaaatgc cgtaccgcat cgatttgttt gacgatgaaa
    13741 tcgacagcat caaaaccttc gataccgaaa cgcaacgcac catttccccc gtttccgaaa
    13801 tccgcctgct gccggcgcac gagttcccca ccgacagcga ggcgcaaaaa atcttccgca
    13861 gccgcttccg cgaggaagtc gatggtaatc cgaacgatgc ggctgtgtac aaagccgtca
    13921 gcaacggtca tttcggcgcg ggcgtggaat actacctgcc gctgtttttt gaaaacgagt
    13981 tggaaacgct gtttgactat atcggcgaag atgcgctgtt tgtctcttta gacgatgttc
    14041 atgccgaggc aaaccgtttt tggagcgatg tcaaatcgcg ttacgcgatg gcgcagggcg
    14101 acgaaaccta tccgcctttg cttccacagt atttgtatct ctctgccgat gtgttcgcag
    14161 gccgtctgaa aaactacgga caggtgctgc ccgatgtttc cggcaaggaa tacaccctgc
    14221 ccgaccttgc cgtcaaccgc caagcagatg agccgttgca ggcattgaag gattttcaga
    14281 cggcgtttga cggacggatt ttgctgtgcg ccgaaagttt gggacggcgc gaaactatgc
    14341 tcggtttctt gcagcaaaac ggtttgaaag ccaaacccgt gtccgactgg cagggctttt
    14401 tatcggcaca cgagccgctg atgattacag tggcgccgtt ggcatacggg ttcaaactgg
    14461 gcggactgca atcgccgaac caacagcaac ctactccctc ccccgtgggg gagggttggg
    14521 gagagggcaa agcagttgcc gctcaaaccg aatttcccgc atccgcaaca aaccctctcc
    14581 ctagccctct cccacaggag agggaacaaa gtgcagccgc cgtttcagac gacctgaaaa
    14641 ccaaaagcag cctgcatccc gtcgcaaata atctgcacgg gcaaatccga cagcaaccta
    14701 ctccctcccc cgtgggggag ggttggggag agggcaaagc agttgccgct caaaccgaat
    14761 tttccgcagc cgcaacaaac cctctcccta gccctctccc acaggagagg gaacaaagtg
    14821 cagccgtcgt ttcagacagt ctgaaagcag ccgccgtttc aaccgaaagc agcctgcccc
    14881 ccggtaaaag taatctgcac gggcaaatcc aacagcaacc tgccccctcc cccgtggggg
    14941 agggttgggg agagggcaaa gcagttgccg ctcaaagtgc catcgccgtc atcaccgaat
    15001 ccgatctcta ccaatacgtc gcccgttcgc gcatccacaa ccgccgcaag aaacacgccg
    15061 ccgtttcaga cgggctgttg cgcgaccttg ccgaaatcaa tatcggcgac cccgtcgtgc
    15121 acgaagaaca cggcatcggg cggtatatgg gcttggtaac gatggacttg ggcggcgaaa
    15181 ccaacgaaat gatgttgctc gaatacgcag gcgaagcgca gctttatgtg cctgtttcgc
    15241 aactgcattt aatcagccgc tactccggtc aggcgcatga aaacattgcc ctgcacaagc
    15301 tcggcagtgg cgcgtggaac aaggcgaagc gcaaagccgc cgaaaaagcg cgcgacaccg
    15361 ccgccgaatt gctcaacctc tacgcccaac gcgccgccca atcgggacac aagtttgaaa
    15421 tcaacgagtt ggactatcag gcgtttgccg acggcttcgg ctacgaggaa accgaagacc
    15481 aggccgccgc catcgccgcc gtgattaaag atttgacgca agcgaagccg atggatcgcc
    15541 ttgtgtgcgg cgatgtcggc ttcggcaaaa ccgaagtcgc cctgcgcgcc gcgtttgtgg
    15601 cggtgatggg cggcaaacag gtcgccgtac ttgctccgac cacgcttttg gtcgagcagc
    15661 acgcgcaaaa cttcgccgac cgtttcgccg atttccccgt gaaagtcgcc agcctttcgc
    15721 gtttcaacaa cagcaaagcc accaaagccg cgctggaagg catggcagac ggcacggtcg
    15781 atattgttat cggtacgcac aaactggtgc aggacgacat caaattcaaa aacttaggtt
    15841 tagtgattat cgacgaagaa caccgcttcg gcgtgcgtca gaaagagcag ctcaaacgcc
    15901 tgcgcgccaa tgttgatatc cttaccatga ccgccacgcc gattccgcgt actttaagta
    15961 tggcgttgga aggactgcgc gacttctcgc tgattaccac cgcgcccagc cgccgcctcg
    16021 ccgtcaaaac ctttgtcaaa ccctttagcg aaggcagcgt gcgcgaagcc gtgttgcgcg
    16081 aactcaaacg cggaggacag gtatttttcc tgcacaatga agtagatacg attgaaaata
    16141 tgcgcgagcg gctggaaacc ctgctgcccg aagcccgcat cggcgtggcg cacggacaac
    16201 tgcgcgagcg cgagctggaa caagtcatgc gcgacttttt gcagcaacga tttaacgtgt
    16261 tgctctgttc caccatcatc gaaaccggta tcgatatccc caacgccaac accatcatca
    16321 tcaaccgcgc cgacaaattc ggactggcgc aactgcacca gcttcgcggg cgcgtcggcc
    16381 gcagccatca ccaagcctac gcctacctgc tcacgcccga atacatcact aaagacgcag
    16441 aaaaacgcct cgatgccatt gcggcggcag acgaactcgg cgcaggtttt accctagcca
    16501 tgcaggattt ggaaatccgt ggtgcaggcg aaatccttgg cgaaggacaa tccggcgaaa
    16561 tgatacaggt cggcttcacg ctctacaccg aaatgctcaa acaagccgtt cgcgacctca
    16621 aaaaaggccg ccagcccgac ctcgacgcac cgttgggcat caccaccgaa atcaaactgc
    16681 acagccccgc cctgctgccc gaagattact gccccgacat ccacgaacgg ctcgtcctct
    16741 acaaacgcct cgccgtctgc gaaaccgtgc aacaaatcaa caccatacac gaagaactcg
    16801 tcgaccgctt cggcctgccc gaacaacccg tcaaaaccct tatcgaaagc caccacttac
    16861 ggcttatggc aaaagaattg ggtatcgatg ccattgatgc ggccggcgaa gcggtaacgg
    16921 taacctttgg taaaaacaat aatgtcgatc caaccgaaat catcctgctg attcagaacg
    16981 acaaaaaata ccgccttgcc ggcgccgata agctgcggtt taccgcagag atggaaaata
    17041 tcgaggtcag aatcaacacc gtaaaaaacg ttttaaaaac cttgcaaaac agatgcctgc
    17101 ccaaataaag ccgacaccgc aatgccgtct gaaacaccgt tttccttgtc cgaaagccgc
    17161 cattatgaat ttgaaggaaa ctccactata atacggcatt cagatttcca gacggcatcg
    17221 cgcccgtcaa accgcacaca aaccaaaagg aaatacatgt tccgtaccat gcttggcgga
    17281 aaaatccacc gcgccaccgt taccgaagcc gatttgaact atgtcggcag tattaccgtc
    17341 gatcaagacc tgttagacgc ggcaggcatc taccccaacg aaaaagtcgc cattgtcaac
    17401 aacaacaacg gcgaacgttt tgaaacctat accattgcag ggaaacgcgg cagcggcgtg
    17461 atttgtctga acggtgctgc agccaggctg gtacagaaag gcgatatcgt catcatcatg
    17521 tcttacgtcc aactctccga acccgaaatc gccgcacacg aacccaaagt cgtcttggta
    17581 gacggaaaca acaaaatccg cgacatcatc tcctacgagc cgccgcacac cgtgctgtaa
    17641 ttccgcaaac ggacatcgat tatggatatt aaaatcaacg acatcaccct cggcaacaac
    17701 tcgcccttcg tcctattcgg cggcatcaac gttttggaaa gcttggattc caccctccaa
    17761 acctgcgcgc attacgtcga agttacccga aaactcggta ttccctatat ctttaaagcc
    17821 tctttcgaca aggcaaaccg ttcctccatc cattcttatc gcggcgtagg cttggaagaa
    17881 ggcttaaaga tttttgaaaa agtcaaagca gagttcggca tccccgtcat taccgacgta
    17941 cacgaacccc atcagtgcca acccgtcgcc gaagtgtgcg atgtcatcca gcttcccgcc
    18001 tttcttgcgc ggcagaccga tttagtggtt gccatggcaa aaactggcaa cgtcgtcaac
    18061 atcaaaaaac ctcagttcct cagcccctct caaatgaaaa acattgtgga aaaattccac
    18121 gaagccggca acgggaaact gattttatgc gaacgcggca gcagcttcgg ctacgacaac
    18181 ctcgttgtcg atatgctcgg tttcggcgtg atgaaacaga cttgcggcaa cctgccggtt
    18241 attttcgacg ttacccattc cctgcaaacc cgcgatgccg gttctgccgc atccggcggt
    18301 cgtcgcgcac aggctttgga tttggcactt gcaggcatgg caacccgcct tgccggtctg
    18361 ttcctcgaat cgcaccccga tccgaaactg gcaaaatgcg acggccccag cgcgctgccg
    18421 ctgcaccttt tagaagattt tttaatccgc atcaaagcat tggacgattt aatcaaatca
    18481 caaccgattt taacaatcga gtaacacggt ttcgccttat gatgcagact ttccgaaaaa
    18541 tcagccggta tgtcgcaacc ttgtggctcg gtatgcagat tatggcgggt tatatcgccg
    18601 caccggtgct gttcaaaatg ctgcccaaaa tgcaggcggg cgaaattgcc ggcgtattgt
    18661 tcgacatcct ctcttggagc gggcttgccg tttggggcgc ggtactggct gccgcctttg
    18721 ccgccctaac ccggcggcaa accgccctgc tgcttttttt attgtccgcc cttgccgcca
    18781 accgattctt gattacaccc gttatcgagg cactgaaata cggacatgaa aattggctgt
    18841 tgtcgtttgt aggcggatcc ttcggaatgt ggcacggcat ttccagtatt gtttttatgg
    18901 caaccgccct actttcagca gttttaagtt ggcggctttc cggcaaagat gccgtctgaa
    18961 gccctcccat ttttttacct cccttcactt cacttggaga acattcatga gcgcaatcgt
    19021 tgatattttc gcccgcgaaa ttttggactc acgcggcaac cccacagtcg agtgtgatgt
    19081 attgctcgaa tccggcgtaa tgggacgcgc agccgtaccg agcggcgcgt ccaccggtca
    19141 aaaagaggct ttggaacttc gcgacggcga caaatcccgt tattcgggca agggcgtatt
    19201 gaaggcggtc gaacacgtca acaaccaaat cgcccaagcc ctcattggta tcgatgccaa
    19261 cgagcaatct tatatcgacc aaatcatgat cgaattggac ggtactgaaa acaaaggcaa
    19321 tttgggtgcg aatgcgactt tggcggtttc tatggcggtt gcacgcgccg ctgccgaaga
    19381 ctcaggcctg ccgctttacc gctacttggg cggcgcaggc ccgatgtccc tgcccgtacc
    19441 gatgatgaac gtcatcaacg gcggcgaaca cgccaacaac agcctgaaca tccaagagtt
    19501 tatgattatg cccgtcggcg caaaatcttt ccgcgaagcg ttgcgctgcg gtgcggaaat
    19561 tttccacgcc ttgaaaaaac tgtgcgacag caaaggcttc ccgaccacag tcggcgacga
    19621 aggcggtttc gcccccaacc tgaacagcca caaagaagcc ctgcaactga tggtcgaggc
    19681 gaccgaagcc gccggctaca aagcgggcga agacgtatta ttcgcattgg actgcgcctc
    19741 cagcgagttc tacaaagacg gcaaatacca cttggaagcc gaaggccgct cctacaccaa
    19801 cgcggaattt gccgaatatc tggaaggcct ggtcaacgag ttccccatca tctccatcga
    19861 agacggcatg gatgaaaacg actgggaagg ctggaaactg ctgaccgaaa aactgggcgg
    19921 tagagttcaa ttggttggcg acgacttgtt cgtaaccaat ccaaaaatct tggccgaagg
    19981 catcgaaaaa ggcgtagcaa acgcattgct ggtcaaagtc aatcaaatcg gtactttgag
    20041 cgagaccctg aaagccgtcg acttagccaa acgcaaccgc tacgccagcg taatgagcca
    20101 ccgctccggc gaaaccgaag acagcaccat tgccgacttg gcagtcgcca ccaactgtat
    20161 gcagatcaaa accggttctt tgagccgttc cgaccgcatg gcgaaataca accaactgct
    20221 gcgtatcgag gaagaattgg cggaagccgc cgactacccc agcaaagccg cattctacca
    20281 actgggcaaa taaaaaaggt taaggtatga agtgggtaac tgtcgtttta tccttcgcac
    20341 ttgtctgttg ccaatacagc ctctggttcg gcaaaggcag catcggacgc aacagcagtc
    20401 tgagagaaca gattgccgtt caagaagaaa aaaaccagac actcgcccta cgcaatcatt
    20461 cccttgccgc cgaagtctat gatttggaaa acggtcaaga agccatttcg gaaatcgccc
    20521 gggtagaact gggttatatc caagacggtg aaacctttta ccgactcatc aggcataacc
    20581 ggtaataccg tcaaaaagcc gtccgaacca atgttcggac ggcttttatt tcaacaaact
    20641 gtcagacagc ccctcatcct cccccgacaa accgcaatcc agcctgacat ccccctcgac
    20701 gcaacagcag cacggcagta tctcgtcccg ccccaaaaaa gccaaaggcg gctcccgata
    20761 agtaacgctt ccctccaaaa tcttcactcg gcacgatccg caatatccgc ttcggcactg
    20821 atattccacc atatgccccg tccgttccaa gccttccaac agagtctcgc cctccaagag
    20881 ttcaaaaaaa cccttattcg taccaatgcg cgccatttcc gaccaatcaa a
//
LOCUS       DAWGRZ010000016        20710 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000016 DAWGRZ010000000
VERSION     DAWGRZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20710
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.016"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(63..239)
                     /locus_tag="WHT44_000420"
     CDS             complement(63..239)
                     /locus_tag="WHT44_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532651.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(242..1609)
                     /locus_tag="WHT44_000421"
     CDS             complement(242..1609)
                     /locus_tag="WHT44_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ2532652.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GERFDAHDFVEDVLAAGAAAVVVSREDCAAMDGALKVDDTLAALQTLAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDDAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPNAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     IALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALALAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGELGEDEAAAMHAEVGAYARDQGIE
                     AAYFVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVE
                     ALEDK"
     gene            complement(1734..3380)
                     /locus_tag="WHT44_000422"
     CDS             complement(1734..3380)
                     /locus_tag="WHT44_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ2532653.1"
                     /translation="MKQSARIKNMNQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSGGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            complement(3403..4881)
                     /locus_tag="WHT44_000423"
     CDS             complement(3403..4881)
                     /locus_tag="WHT44_000423"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ2532654.1"
                     /translation="MFSKLTPLAETNIPSLLCENAAGRLLHSDSRQIKQGDIFIACPG
                     EYADGRSYIPTAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIIGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRRQGATAAAMEVSSHGLDQSRVNGVPFCSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLQHAVINVDDGYGAELIGRLKKDCPDLAVYSYGFSEQADIRIIDF
                     TASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLEKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALSTLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPQDIINDILPAVPNPECVEADRAAAIRYAVG
                     RAAASDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4906..6654)
                     /locus_tag="WHT44_000424"
     CDS             complement(4906..6654)
                     /locus_tag="WHT44_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ2532655.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLYAVPKDM
                     EEMPTAAQLEHLSELIDVPVDVLRSKLDQKNKSFIWIKRQLDHKLAEEVEALGLKHFA
                     FQKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHAEEGAEVVLRDREGNIV
                     DSLDSPRNKAPQNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALVNTPAYEPNKPGQADSEQRRNRAVTDMIEPGSAIKPFVIAKALDADKTNLNERLNT
                     QPYKIGPAQVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFSSKEMYDLYHELGIGVRM
                     HSGFPGESAGALRNWQKWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKASTARQVRELMVSVTEPGGTGTAGAVDGFDVGAKTGTARKLVNGR
                     YVDYKHVATFIGFAPAKNPRVIVAVTIDEPTANGYYGGVVTGPVFKQVMGGSLNILGV
                     SPTKPLTNVAAVKTPS"
     gene            complement(6715..6978)
                     /gene="ftsL"
                     /locus_tag="WHT44_000425"
     CDS             complement(6715..6978)
                     /gene="ftsL"
                     /locus_tag="WHT44_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ2532656.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(6975..7961)
                     /gene="rsmH"
                     /locus_tag="WHT44_000426"
     CDS             complement(6975..7961)
                     /gene="rsmH"
                     /locus_tag="WHT44_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ2532657.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRRIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PELPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            complement(7958..8413)
                     /gene="mraZ"
                     /locus_tag="WHT44_000427"
     CDS             complement(7958..8413)
                     /gene="mraZ"
                     /locus_tag="WHT44_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ2532658.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVSAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            8692..9903
                     /locus_tag="WHT44_000428"
     CDS             8692..9903
                     /locus_tag="WHT44_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ2532659.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPSPEARQFSLKLQTLIAE
                     KIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLARQL
                     QELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQARAP
                     EASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTYSA
                     RPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLEHGCMIFIDYGFDAAQ
                     YYHPQRNQGTLIGHYRHHIIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYLPQ
                     SHFLLNLGITELLAQTGKTDSAAYICEAAAVQKLIDQHEMGELFKVIAFGKNIGIDWA
                     GFRFGDICHKL"
     gene            10035..10111
                     /locus_tag="WHT44_000429"
     tRNA            10035..10111
                     /locus_tag="WHT44_000429"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10069..10071,aa:Met,seq:cat)
     gene            10119..10194
                     /locus_tag="WHT44_000430"
     tRNA            10119..10194
                     /locus_tag="WHT44_000430"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10152..10154,aa:Ala,seq:ggc)
     gene            complement(10476..11297)
                     /locus_tag="WHT44_000431"
     CDS             complement(10476..11297)
                     /locus_tag="WHT44_000431"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ2532660.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGLGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(11330..11974)
                     /locus_tag="WHT44_000432"
     CDS             complement(11330..11974)
                     /locus_tag="WHT44_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ2532661.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRSVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYRIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(11977..12840)
                     /gene="ftsN"
                     /locus_tag="WHT44_000433"
     CDS             complement(11977..12840)
                     /gene="ftsN"
                     /locus_tag="WHT44_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ2532662.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAATEAEQSDAEKAADKQPVADKA
                     DEVEEKAGEPEREEPDGQAVRKKALTEEREQTVREKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAAKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTSDKAEATHYLQMG
                     AYADRQSAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHE
                     VASLIRSIESK"
     gene            complement(12946..14442)
                     /locus_tag="WHT44_000434"
     CDS             complement(12946..14442)
                     /locus_tag="WHT44_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ2532663.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDMEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLE
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECQVPQMPFEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDTSARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14456..14776)
                     /locus_tag="WHT44_000435"
     CDS             complement(14456..14776)
                     /locus_tag="WHT44_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ2532664.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLEKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15259..16785
                     /gene="putP"
                     /locus_tag="WHT44_000436"
     CDS             15259..16785
                     /gene="putP"
                     /locus_tag="WHT44_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ2532665.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRATR
                     "
     gene            complement(16875..17042)
                     /locus_tag="WHT44_000437"
     CDS             complement(16875..17042)
                     /locus_tag="WHT44_000437"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532666.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            17034..20639
                     /gene="putA"
                     /locus_tag="WHT44_000438"
     CDS             17034..20639
                     /gene="putA"
                     /locus_tag="WHT44_000438"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ2532667.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRTQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSKKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDNVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRAGEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTELAASGIQAVVEPDSPLASYTADLEGLLLVNGKPETAGISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4908 a         5945 c         4989 g         4868 t
ORIGIN      
        1 cgggcctgcg cccgtttgag gttggcttgc cgcccggcaa ggtttcggac ggcttttgca
       61 gattaacgtg cagggacttt ctcaatcacg caaattgggt aactccccgg atttttaccg
      121 ccgcccaagt ctaaacataa atcttcataa agatacgcct gcgtccgcat tccggcataa
      181 aacgcgccga ccagcgcggc aatcagcaat aaggctttaa aaaaacgtcg gcttttcatt
      241 ttcacttatc ctccaatgcc tcgaccactt cttccatctg cataaagcgc gaacctttca
      301 ccaacacggt ggcgcgttcg ggcaaatcgt ggcgcaacac ttgaatcaac gggtctttgg
      361 cggcgaacca caaaccgtcc gcgccaaatt tttccgccgc ttcgacgctg ttgtcgccga
      421 caaaataagc cgcttcgatg ccttggtcgc gggcatacgc gccgacttcg gcgtgcatag
      481 cggcggcttc gtcctcgccc agttcgccca gttcgcccat atcgcccatc acgaaaatac
      541 gcggcgcagg catacgcgcc aacacgtcaa tcgcagcttt catgctgtca gggttcgcat
      601 tataagtatc gtcaatcagg gttgcgccct tgattccgga tttgacgttc agacggcctt
      661 tgatattgct gaagcctttc aaaccttccg ccacatcgtt caaactcaaa cccgcagcca
      721 aagccagcgc ggcggcagcg gcggcgttgt ggacattgtg gcggccggga acaggcagca
      781 ccacggcggc gcgctcatcg ccgcacacca aatcaaattc gcacgacaac ggtttcagca
      841 caatattttc cgcgtgaaca tcgccgctat cgatgccgaa agtgcgcgta ttcaaattaa
      901 gcgttgccgt tttgaagaca gccatattgg catcttcttg aggaatcagt gcaatgccgt
      961 ctgaacataa accttggtaa atctcgcttt tcgctttggc aatatcgccc actccgtcga
     1021 aaccgcagcc gacatgggcg cgcatggcgt tgttgaccaa tgcggcattt ggtttggcga
     1081 tttgcgttaa aaccgccagt tcgccgaaat ggttcatgcc catttcaatc acggcatagc
     1141 ggtgtttttc gtttaacttc aacaaagtca gcggcaatcc gatatggttg ttgaagttgc
     1201 ctgccgtcgc caacacggca tcatcgccga aacggcggcg caataccgca gccagcattt
     1261 ccttcaccgt cgtcttgccg cccgaaccgg taatgccgaa cacaaacgga ttcacatttt
     1321 cacgccacgc ctttgccagc gtttgcaatg cggcaagcgt gtcatcgact ttcaacgcgc
     1381 catccattgc agcacaatct tcgcgcgaaa ccacaaccgc cgccgcacca gcagccaata
     1441 cgtcttcaac aaaatcatgc gcgtcaaacc gctcgcccgc caatgcgaaa aacacatcgc
     1501 ccgcgcggat gtcgcggctg tcggttacga tgcgcgacac gggtttgctt tcagacggca
     1561 tcggaagctt gagggcttgg cagatgaaat ttaggtccag tggtttcata tttactttcg
     1621 ttaatattcg ggcggcggac acatcggtag cggctgattt ttttatcgcc tgttttgctg
     1681 tggtaaaaca cagattattt tcccattctc attcggcatt ttttctgtac gtatcatttt
     1741 ttagacgtat ttttagtcga tttgcctttt cccgcatacc acggcgcggg gtcgtcgggc
     1801 agtccgtcga taaaggcaag gttattgcct tcgccctgca catcgggaac attcccccaa
     1861 aaatcatagc cgtcatcggg caactcgtcg gtttcgatac ccgtccaact gccgaatccg
     1921 cgtaaaaaat taaacgcgct gcgcctcact ttaatcattt cgcgccgcgt gtcatcggac
     1981 gaaattttaa ccagcggcac gccgtagctt tgacgcttcc acgcaccatg tgtcaacgtt
     2041 cgctcgccgc ctttaccgac atgcatcaaa ccgtggtcgg aaaagtaaac caaagaccag
     2101 ctttctttat tttcattcaa tatcttaact gtatcttcta aaaatttatc ggtttgcgcg
     2161 atggtggaaa catagcagga tattttttca gtttgatact gaaaccgccg cgcatcctta
     2221 tccaaacgtg tgcaaaaatc actgtgcgaa cccatcaggt gcatcacaat cagccgaggc
     2281 ttcgtgcctg catttcctat caaaacccgt ttgaacgccg gcaacaaaag gcggtcgctc
     2341 aaccccgcgc ttttgccata atcacccctt tgggtaaacc acggataatc gctgcgtagg
     2401 gcataggtgg aaatttcgtt ggcaaaatgc cccaacattc cttgattaga cagccacgcc
     2461 gtccgaaaac ccgcctgctt cgccaagctg acgatgttat tgttcggttc tcccggcagc
     2521 cccaaagtct gcggcagcga aagattcgtc gcgtgggcgg tcgattggta accgtttatc
     2581 aacagccctt tggtctgact caaaaaaggc gtatcgggca atgggaaacc gtaaacattc
     2641 atataatccg aacgcgcgct ctcaccgata accacaacat aatttttata cttgggcgca
     2701 acatgacgga tatgccatgt tgacgctttt tttgctgctt ccaaaatgtg ggcgcgcttg
     2761 gcggcatatt gtgccggcgc ggaagccaaa tcgtaataca ggtcgaaaat attcaacaac
     2821 aggccggcat caggttctcg caaatcttta tcgctggcga ttttatccat caccgcgcag
     2881 gacaaaatca aagtcaataa tatagtcagc catattttgc tgcggcgttt atagtttttt
     2941 acgtcagcaa ataccccccc cgatacacaa tatttccaaa ctgtcaagcc aaaaatgaaa
     3001 aataatgcct gcacaaaata aagcgacccg ggaagattgc cgacaaattc acgcgcctcg
     3061 gcaggattgc tttccaatat cgaaccgact atctgataag acggcgcacc atacagccag
     3121 ccgaccggca aatatagggc ggttgtgccg acgtaaatca gtaaaacaac tgaagaaacg
     3181 cgcgggaaac cgcgtgccaa taataataaa aatacaaccg aagccaaagc acccacggca
     3241 gaataacggt agccgtattc atattccaag tgataacctg atgcgatggc cgcgccaaaa
     3301 caaaaggcta aaagcgcgca aatgcccaat gtatttttta atgtctgatt catatttttt
     3361 attcgggcgg attgtttcat acgatgccgt ccgaatatcc cattatttac gagttaacaa
     3421 agcctgtccg acgatttcaa gatcggaaaa acggtgcttc accccttgta catcctgata
     3481 gttctcatgc cctttgccgg caatcagaat gatgtcgctt gcggcggcac gtccgactgc
     3541 ataacggatg gcggcggcgc ggtcggcttc gacgcattcg ggattaggta cggcaggcag
     3601 gatgtcgttg atgatgtctt gcggattttc caaacgcggg ttgtcgctgg ttacgacgac
     3661 tttatccgcg ccctgtacgg ctgccgcgcc catcagcggg cgtttgccgc gatcgcggtt
     3721 gccgccgcag ccgaatacgc accacaaagc cgcaccctgc ggtttgattt cctgcaaggt
     3781 ggagagtgct ttttccaatg cgtcgggcgt gtgggcgtaa tcgacaacga ccaagggctt
     3841 gccgctgttc atgatgcagt ccatgcgccc tgaagcggga cggatttttt ccagcacatc
     3901 caatacctta tccagcggat agccgttggc acacagcaag gcgatgcagg cggcgaggtt
     3961 ttgcgcgttg aaccgtccga gcaggcgcgt gcggcatttc ccttcgcccc acggggtttg
     4021 gaatacggct gctatgccgt ctgaagaggc ggtgaaatcg ataatgcgga tgtcggcttg
     4081 ctcgctgaag ccgtaactgt aaacggctaa atcggggcag tcttttttca gacggcctat
     4141 gagttccgcg ccgtatccgt catctacatt gataacggcg tgttgcaggc cgtgccagta
     4201 aaacaggcgc gacttgatag cgccgtaggc ttccatcgtg ccgtggtagt caaggtggtc
     4261 gcgtgtgaga ttggtaaaga ttgcgctgca aaacggcaca ccgttgacgc gcgactggtc
     4321 aagcccgtgg ctggagactt ccatcgcggc ggctgttgca ccttgccggc ggaaacggta
     4381 gagcagggtt tggacatcga cgggggcggg ggtggtgtgc gtggtttctt ccaacgcgcc
     4441 ccaaaagccg ttgccgaccg tgccgataat ggcggttttt tcgcccaaca aatcggcggc
     4501 ctgcgccagc cattgcgtaa tggaggtttt gccgttggta ccggttacgc cccagacttt
     4561 gaggccgtct gaaacgttgc cgtaaacctg tgccgccaat atgccggcgc ggtgtttcaa
     4621 atctttgatg ccttgattgg ggactttcca ttcgggattc cacgcgaatt tgccgtcgtc
     4681 gtcccaaaaa acaaaagccg cgccgttggc aacggcggtg gggatataac tgcggccgtc
     4741 ggcatattcg cccggacagg caatgaaaat atcgccttgt ttgatttgac ggctgtctga
     4801 atgcaacaaa cgccctgctg cgttttcaca cagcagagac ggaatgttgg tttcagctaa
     4861 aggggttaac ttgctgaaca taaaataatc tcgttgatac tcggattaag acggtgtttt
     4921 gacggctgca acattggtca gaggtttggt cggagaaacg cccaagatgt tcaggctacc
     4981 gcccataact tgtttgaaga ccggacctgt cactacgccg ccgtagtaac cgtttgcagt
     5041 cggctcgtca atggttaccg ccacaatcac acgcggattt ttagccgggg caaaaccgat
     5101 gaaagtggca acgtgtttgt aatcgacgta acgaccgtta accaacttac gcgccgtacc
     5161 ggtttttgcg ccgacgtcga aaccatctac cgcacccgcc gtacccgtac cgccaggttc
     5221 ggttacagaa accatcaact cacgcacctg acgtgcggtc gatgctttaa atatacgttt
     5281 gccttgcggc gcaaccgcct gtttttcaaa gctgaccggc agcaaaacgc cgtcgtgcgt
     5341 cagtgcggta taggcacgcg ccaattgcag caggctcaat tgcaggccgt aaccgaaaga
     5401 catggttgcc tgttcgatag gccgccattt ctgccaattg cgcaacgcgc ccgcactttc
     5461 tcccggaaaa ccggaatgca tacgcacacc gatgcccaat tcatgataca aatcatacat
     5521 ttctttcgaa ctgaatcgtg cagacagttt gcttgtaccg acattggaag atttctgcat
     5581 gataccgcgc acatccaaag aaggataaac atgggtatcg cgcacctgag ccggaccaat
     5641 tttataaggc tgcgtattca acctctcgtt caaattggtt ttatccgcat ccagtgcttt
     5701 cgcaatgaca aagggcttta tcgccgaacc gggctcgatc atgtcggtaa cggcacggtt
     5761 acgcctctgt tcgctgtctg cctgaccggg tttgttgggc tcataggcag gcgtattgac
     5821 caaggcaagg atttccccag tacgggcatc caacaccacc accgttccgg cttttgcctg
     5881 atggtattcg accgccttat tcaactcttc ataagccaaa gtctgaatcc tctgatccag
     5941 agaaagaata atgtctttgc cgttctgcgg ggctttatta cgcggagaat ccaaactgtc
     6001 cacaatattg ccttcccgat cccgcaatac gacttccgca ccttcttcag catgcaaact
     6061 gtcttcaagc gaaagttcca aaccttcctg acctttgccg tcaatatcgg taaatccgat
     6121 gacatgtgca aaaaggttgc ccatcgggta atggcgtttt aattcctttt gaaatgcaaa
     6181 atgtttcaaa cctaaggctt ccacctcttc ggcaagcttg tggtcgagct gacgcttgat
     6241 ccagataaac gatttgtttt tctgatcaag tttgctcctt aaaacatcaa ccggaacatc
     6301 gataagctcg gaaagatgtt ccaattgcgc cgcagttggc atctcctcca tatctttggg
     6361 aacggcatac agagattctg taggtgcact caatgccaaa accgcaccgt tccggtccga
     6421 aaccgtaccg cgtgtagccg gcaatgtttg agtccgcaca atccggttgt cgccctgttc
     6481 tttcaaaaag ttatacgtta ccgtctgcag atacagtccg cgagcaatca gaccggcaaa
     6541 caagaccgct attgccatca ggacgaagct gatccgtccg ttactggtca tcggcttttt
     6601 gacctgctct tctttgggca gcatccgagg cttatattcg ctcttaatca acatttttac
     6661 ttctcgttat tattatcctg acgcaggaat ccgattccgg cacacaggct gcttctatct
     6721 ttgatgctcc accataaagg tattgcccga aaccggcgga tggaggtttt gtttttctgc
     6781 cgccgccctg atcgcttcgt ggttcgccaa acgcgcctgt tgcagcctca tttgcgcata
     6841 atcctgctcc aaggcgattt cctgtttttt cgccttatcc aaagctgtga aattgagcct
     6901 gtactggttt tgctgcatca caacggaaaa agcggaaacg cacaccgcaa gcagcagaag
     6961 gaaattcaat ttgttcattg ccattcagac gtttttctct gtgattgttc cggtatcgga
     7021 ccggcagtcc gctccgccac acgcaaaacc gcacttctcg ccctcggatt ggcggcaatt
     7081 tccgcctcac ccggctttaa tgccctgccc acgattttca ggggcagctc gggcaaatcc
     7141 gcttccctga ccgccgccca gcgcggcagg ggcgcgtgtt gcgaatattt tttgacaaac
     7201 tgcttcacaa tgcgatcttc caacgaatgg aaagcaatga ccgccaaacg tccgccctct
     7261 ttcagacgac acatgacctg cggcaatact gcccctactt cttcaagctc gcggttaata
     7321 aagatgcgga ccgcctggaa ggtgcgcgtc gcaggatcct gcccccgctc gcgagtacgg
     7381 acgttttgtg ccacgatctg cgccagcttg cgggttgtat cgattggact ttccgcccgt
     7441 tgcgcaacaa tggcgcgcgc aatccggcgg ctaaaccgct cttcaccata attcttgatt
     7501 acctcgtgca aatcctgttc cgacgcaacc gctatccact ctgcggcaga cataccgcgc
     7561 gtcgtatcca tacgcatatc caaaggggca tcgaaacgga agctgaaacc gcggctgccg
     7621 tcatcgattt gcggggacga aatccccaaa tcaaacagcg caccgtccac cttgccgata
     7681 cccaaaccgt ccaatgccgt ctgaaacgaa gcaaaaccgc catgcacgac accgacccgt
     7741 ttgtccgaac gcgccagctc ttctgccaca gcaatcgcct gcgggtcttt gtcgaaaaca
     7801 atcaaccgcc ccgcatcgcc caaacgcgac aaaatcagcc gggaatgccc tcccctgccg
     7861 aacgtaccgt ccacatagac accgtcttcg cgcacggcaa gcgcatccac cgcctcattc
     7921 agcaagaccg tgatatgccg gtaactttct gctccactca caattgcaaa tccgtctgac
     7981 tcaactggaa ggcaagttcg tcaggatcgt catccaaagc ctgaaccatc tcagcctccc
     8041 actgctcgcg accccaaagc tccaaacggt tggcacgacc gaccaaaacg acttcacggt
     8101 cgaaatccac cctcttcctc agtccggcag aaaccagcac ccggccggcg ctgtcccatt
     8161 ccaaaatttc cgcgttatgc agcaaaagat tttgaaaccg ccgcaaaaca gggttatccg
     8221 ccacttttaa gtttaaaagt tgcgccgcaa ccttttccca ctccgcaaca gggtacatca
     8281 acagcttgtg tttcgactcg agcgttacca ctacggcagg cgtatagagg cgcgacagaa
     8341 tgtcacggaa tttggcagga acagccaacc gccccttact gtcgatgctt aattcgtgtg
     8401 cgccgccgaa catgacatgt cccaagccga aatcaaaatc acaagggtaa aagagacact
     8461 ttgccccaca attccccacc aatcgacact ataagaaatt ttaaacactc ggtcaaatca
     8521 gggcatgaaa actcattaac atatctgaaa tttttattcc ttttaaaaac aataagataa
     8581 aaaatgacga caacggcacg gcgggtgcgg tacagaataa tcgaaccaat aaacaactat
     8641 atatgattaa tttaataata taaacacaat atatagtatt aagataaagc catgacagca
     8701 cccgtaccaa cgtgtaatat gtcgggaaat ccaataaatt tacacaagct aacacttatc
     8761 atgcccctcc cctctcccga agcacggcaa ttctcgctca aactgcaaac cctcattgcc
     8821 gaaaaaatcg gcaaacacgg caactggatt ccattttcac gttttatgga attggtttta
     8881 tacgctccgc aatacggcta ctacaccggc ggcagccata aaatcggcaa taccggggat
     8941 tttattaccg caccgaccct cacctctctg tttgcacaga cactggcacg ccaacttcaa
     9001 gaacttctat cccaaacggc gggcaatatc tatgaattcg gcgcgggaac cggacagctt
     9061 gccgccgatt tgttgggcag catttcggac ggcatcagcc gttactatat tattgaaata
     9121 tcgccggagc tggcagcacg tcagaaaaac ctgattcaag cacgcgcacc ggaagcatct
     9181 caaaaagttg tccacttgac cgcacttccc gaagcgtttg acggcatcat catcggcaac
     9241 gaagtactcg atgccatgcc tgtcgaaatc gtccgtaaaa atgaaggcgg ctcattcgag
     9301 catgtcggcg tttgcctaga taatgaccgt tttacctatt cggcacgacc gctgcacgac
     9361 ttgcagctat ctgccttggc ttccctctat tttcctcaaa cagattatcc ctataccagc
     9421 gaactacatc cgcaacaata tgcctttatc cgcacccttg cctcaagact cgaacacggc
     9481 tgcatgatat tcatcgacta cggttttgat gcagcgcagt attaccaccc tcaacgcaat
     9541 caaggtactc tgatcggaca ctaccgacat cacattatcc acaatccttt tgacttcatc
     9601 ggattggccg acctgaccgc acatgtcaac tttaccgaca ttgcacaagc agggacggat
     9661 gccggattag atttgatagg ttaccttccc caatcccatt tcttattgaa cttgggcatt
     9721 accgagctat tggcacagac ggggaaaacg gattcggcag cctacatctg tgaagctgct
     9781 gccgttcaga aactgattga ccagcatgaa atgggcgaac tgtttaaagt catcgcattc
     9841 ggaaaaaata tcggcatcga ctgggcagga ttccgcttcg gcgacatctg ccacaaactc
     9901 taaccctcat gccgcctgaa tccgcttcag acggcataaa ctttttaaca tttaaaaaca
     9961 gtcaactaat tcaaaattaa aaaatacggc ttgtcaaaaa aacagaaaaa catataatag
    10021 cgtcttcacg aaacggcgaa ttagctcagt cggttagagc agaggaatca taatccttgt
    10081 gtccggggtt cgagtccctg attcgccacc aaattttcgg gggtatagct cagttggtag
    10141 agcgcttgca tggcatgcaa gaggtcagcg gttcgatccc gcttacctcc accagataaa
    10201 aaagcacaga ccgtaaaaag gtatgtgctt ttttattgcc tgattgccag caaataaaga
    10261 ataaaccact gccttcaaaa caggcaatcg actttaaacc tatcgccccg cctgtcctga
    10321 ttttatagtg aattaaattt aaaccggtac agcgttggct cgccttgccg tactatctgc
    10381 ggcttcgtcg ccttgtcctg atttttgtta attcactata tcagcccgcc agacaaaccc
    10441 gacccgaata atgtcttcag gtcgggttta tggtttcatt cccaacttat ccagcctgac
    10501 agccacaata taatgatggc aataccaaaa acaatgcggt aataggcaaa aggaatataa
    10561 tttttcttgg aaacaaacct cagcaacgct tttaccgcta ccaagcctga aacaaaggca
    10621 gcaataaagc ctatcagaat caaaccgaca tcatgcaggg tgaaaaatcg gtaatgtttc
    10681 aggacatcat aagccgttgc ggcaaccatc atcggcacag ccaagaaaaa cgagaattct
    10741 gtcgcagttt tccgttcgat gccccaaagc atcccgccca taatcgtact gcccgaacgg
    10801 gacgtacccg gaaccagtgc aaacacttgg gcaacgccga tcatcaaggc atcaatcgga
    10861 cgcaatgcat caacatcggc aattttaggc tctgctcggc tttggcgttt ctccacccac
    10921 aaaataaaaa aaccgcccaa aaccagcatg actgcaacac tcaaggggtt aaacagatac
    10981 tctttgattt gtttgccgaa caacagcccc atcacggcgg caggtataaa agcaatggca
    11041 agattaagga cgaagcggtt ggctttccgg tcttttccca agccgtgcaa cacattgctg
    11101 aaacgttgcc ggtattcaaa cactaccgcc aaaactgcac cgagctggat ggcaatttca
    11161 aaaaccttgt gattgctgtg aaaaccaatc agattgccga acacaatcaa atgtccggtg
    11221 ctggaaatcg gtaaaaattc ggtaaaacct tctaccaagc ccatcatcag ggctttcagg
    11281 acaatcagaa aatccattgc ttgcgcttct ttcggatacg ggagttcggc tatttctgta
    11341 cagcaggggt ctgacgcttg cgttcttccc tgactttggc aaccaattct ttaatcgtat
    11401 gaacgccgcc gtcaaagccg ttattgaaga taacgcggta ttttccgccg acaataacgg
    11461 tcggcgtgct gtcgatgcgg tattgttccg tcagtttctg catttttaat gcggcggcgg
    11521 cagcttcggg ggaatcatag gcgcgcatca gttttttgcc gtcaaagcct ttttgagaca
    11581 aagcccattt tccggcaacc gacctgtttt ccaagcggat tttttgttcg taaactgctt
    11641 taaacacagc agggtttgcc tgatatttca aacccgacaa attgacggca gccgccatcc
    11701 tagccaaacc gagcatttca ggctgccaga ccacgtgctc cgtcctcaaa taggcatcag
    11761 acggcaatgc cttgcccagt ttcaataaca aaggatcgaa atgatggcaa tgtacgcaga
    11821 aatagccgaa aaattccaaa acctcaattt tacccgactg ttcttgagga atgggtttat
    11881 ccaacacaag atagtcttcc ccttccgtca gggcatatgc ctgcgcggac aacactgccg
    11941 acagcagcag cggcaacaga tgtttgagct tcataattat ttgctttcga tagaacggat
    12001 caggctggcg acttcatgtt ttttcaactc gtcctgcatt tttttcaccg catcggcaga
    12061 catattgccg ctttgcaccc ggtaaagcgt tttatgtccc gcctgataac cgaccacctt
    12121 ggaagatatg cccaagattg ccagtttggc acgctgccct tccgcgctct gacggtcggc
    12181 atacgcgccc atttgcagat aatgcgttgc ttccgccttg tcggacgttt tcattttctg
    12241 cacttctttg gcggcggcac tgcgcgcttt ttcgatgctg ccgctgttga ggatttgttc
    12301 cggggttggt ttgggtgcaa ctttttcctt cgccgccttt ttctcttctt ttgaagcttt
    12361 tttctctgtt tctttagacg gttttaccgc ttgtttttta accgtttcgg catctttctt
    12421 ctgcgctttt tccctgacgg tttgttcacg ctcttccgtc agcgctttct tacgcactgc
    12481 ctgtccgtcc ggctcttccc gttccggctc gcccgccttt tcttcaacct cgtcggcttt
    12541 atcggcaacg ggctgcttgt cggcagcttt ttccgcatcc gactgctctg cctctgtcgc
    12601 agcatccggt tcggacaagg cgttttgatc ggccggttca ggttggatgt cttccttagg
    12661 ctggtttttc ggtttcagga tttccgtttc tgcaggctgc ttcgacgaag ccgggatttt
    12721 gaacgcattt tgaccgctct ggttcagata aaacaaaata ccggcaataa tgaccgtcgc
    12781 cagtatcaaa ccgaagaaaa aaccggacag accttttccg gattgggaaa atttgttcat
    12841 aaacatacct taatgtgttt cagacggcat taacgccgtt tgcttacggg cggatattct
    12901 aacaatatcg ccatatttgg gcaaaacctg cttccattcc cattcctata aagcacgacg
    12961 gaaacctatg gctttcatga cgtggcttct gccgacttct tcgtcgcccg ccaaatccgc
    13021 caatgtacgc gccacgcgca taatgcggtg gaagctgcgg gcggaaaggg agagtttttc
    13081 cagcaggccg cccaatgctt cctgcgcttc tttttgaatg cgggcggatg tgtcgagttc
    13141 actgacactc aaggcggcat tcactttgcc ttgccgtgcg tattgtttgt ctctagcggc
    13201 gataacgcgt tccaaaacgg acgcgctgct ttcccctgct tcctgctgca tcagttcggc
    13261 ggcggacagg ctcgggactt cgatggtcaa atcgatgcgg tcgagcagcg gcccggaaat
    13321 cttgctgcgg taacgcgcga cgctttcggg cgtgcagcgg cagggtttga cgggatgccc
    13381 gagataaccg cacgggcagg ggttcatggc ggcaacaagt tggaatttgg caggatagac
    13441 ggcttggcgc gccgcgcggg aaatgtggat ttcgccgttt tccaacggtt cgcgcaaaac
    13501 ttccaaaact ttgcggtcaa actcgggcag ctcgtccaaa aacaaaacgc cgtggtgcgc
    13561 caatgaaatt tcgcccggac gcggatccga accgccgccg accatagccg ccgcgctggc
    13621 gctgtgatgc ggactgcgga aaggacggtt gctgtcgagt tgttgttggt ggttgggcag
    13681 gagcgaacgc aatgcccaaa cttctaccaa ttcgtcttcg gtcagcggcg gcaggatgcc
    13741 gggcagccgt tgggagagca tagacttgcc cgttcccggc ggacccatca tcaagaggct
    13801 gtgtccgcct gcggcagcga tttccaaagc aaggcgcgcg gtgtgctgac ctttcacatc
    13861 gcacaaatca ggttgtccgc catgttcaaa cggcatctga ggaacttggc attcggtttg
    13921 cgccaaaggt tcgatgccgt tcaaatgggc ggcgacttcg cccaaagagc gcgcgccgta
    13981 aacggtaatg ccgcgcatca cggcggcttg tcctgcattt tcttcaggca aaacaaatgc
    14041 acgttttgcc tgcataccct gccacgccat cgccaacgcg ccacgcacgg ggcgcaacag
    14101 ccccgacagt gccaattccc ccgcaaactc gtattcctcc agtttttcgg gcgcaacctg
    14161 ccccgatgcg gcaaggatgc cgattgcaat cggcaaatcg aaacgccccg actctttggg
    14221 caggtcggcg ggggcgaggt tgacggtaat ttttttggcg gggaattcaa aaccgctttg
    14281 aataatggcg gcacggacac ggtcgcgact ttcctttact tccatatcgg gcagtccgac
    14341 gatgttgaaa tgtggcaggc cgttggcaag gtgggcttcc acttcgacca acggcgcatt
    14401 cataccgctc aaggcgcggc tgtaaaccaa ggcaagcgac atatttcaga cgaccttatt
    14461 cgccggcttc ggtttgctgc ctgatttcgg cgacggcttc ttcggcagcg gcttcagccg
    14521 cttccaatgc tgcccgttcg ggattttgcg cggcttcgag tttttccaaa cgcgcttcca
    14581 aagccgccag tttggtacgg gttttgatta aaacctgctg ctggatgtcg aattcttcgc
    14641 gcgtaaccag atccatacgg ttgaacgcgc cgcccagcat cgccttaata tttttttcca
    14701 catctttggc agggctgttg gcgatggttt cgctgatttt cgagccgact tcctcaaaaa
    14761 gctgcttgcc gaacataatc tgtatccttc ctgaacatat caaattcaat cggctattgt
    14821 ataaggaaaa atgccgtctg aaaacgggcg gcggataatc ggcaaaacat accgcgcctc
    14881 ctttgcggtt gctaaattac acctcaaaac acctgccgcc tgaaaacgga tttcatattc
    14941 cgcgcaacgc ccgattacaa gacactacaa aacaatatgc tgttttaaat gatttttccg
    15001 acgcgcatcg tttcaaactc ggctttttaa gccattaagt gctttgcaaa caacagaaat
    15061 tgggttatcc tgaaacggat tatttacaat ttcatatagt tttattacat atcttattgt
    15121 gattgaagat aatttatccg aatccccctt tcgggtatcc ggattttccg ttgtactttt
    15181 attagaaaaa catttcaggc gcaagttgct tgcaatttca aagcctgtaa ttctgaagtt
    15241 ttagacgagg aatagcacat gaatcccatg tacattactt ttgcaatcta tctggttgca
    15301 gtccttctca tcgggcttgc cgcctatttt tctacgcgca atttcgacga ttatattttg
    15361 ggcgggcgca gcctaggccc gtttgttacc gcgatgtcgg caggcgcgtc cgatatgtcc
    15421 ggctggcttt tgatgggtct gccgggcgcg atttatttga gcggtttgaa tgaggcttgg
    15481 attgccatcg gcctcttggt cggcgcgtat ttcaactggc ttttggtggc gggccgtctg
    15541 cgcgtacata ccgaatacgc caacaacgcg ctgacgctgc cggattattt cttccaccgc
    15601 tttggcgcgg gcggacactt gatgaaagtg gtttccgcac tgattatcct gtttttcttc
    15661 acgatttatt gcgcctcggg cattgtggcg ggcgcaaccc tgttccaaag cctgtttgaa
    15721 ggtatgactt acaatcaggc aatgtggctg ggcgcgggcg cgaccatcgc ctataccttc
    15781 ttgggcggct ttttggcggt aagctggacg gatacgctgc aggcttcttt gatgattttc
    15841 gcgctgattt taacgcctgt gatggtctat ctgggcttgg gcggcgcgga acagatgtct
    15901 gccgcgattc aaagtgtcgc cgcaggcacg ggcaaagaat acggcagcct gtttgccggt
    15961 acgaccgtca tcggcatcat ttccaccgcc gcatggggct tgggctattt cggacagccg
    16021 cacattttgg cgcgctttat ggcggccgaa agcgcgaaat cgctggtatc cgcacgccgc
    16081 atcggtatga cttggatggc gttgtgcctt gcgggcgcgg tagcggtcgg ttatttcggc
    16141 attgcgtatt tcggtgcaaa tcccgacaaa gtttcttcta tgagcggcaa ccacgaacgc
    16201 atcttcatcg cgctttccac cctgctgttc aacccttgga ttgccggcat tattttgagc
    16261 gcgattttgg cggcggtgat gtccaccctg tcctgccagc ttttggtttg ctccagcgcg
    16321 attaccgaag acttctacaa aggctttttg cgtaaaaacg cgcaacagtc ggaattggta
    16381 tgggtcggcc gcctgatggt cttggcgatt gccgtgattt ccatcctgat tgcttccgat
    16441 ccgaacagca aagtattggg actggtgtct tacgcgtggg caggtttcgg cgcggcattc
    16501 ggcccgattg tgattctgtc cgtattgtgg aaacgcatca ctgcctacgg cgcgttgtcg
    16561 ggtatggttg caggtgcgtt aaccgtagtc gtatgggcgg aatgggtgaa aaaaccggct
    16621 caagcggcag gagaaagcgg cttgttgacg atgtacgaaa tcgtgccggg ctttattgtt
    16681 tgcttgatcg ttgccgtgtt ggtttccctg ttcaacaaag aaccttcgcg cgaaacccaa
    16741 gaacgctttg aaaaagccga cgcagattac cgcgccaccc gataaacccc tttcagacgg
    16801 cacggcatcc cggttcgtgc cgtctgaacc cgtttccggg tcaaacacaa ataaaccttg
    16861 cctgcataca ggccttattt gtgttttacc cgtttcattt tcaaagaaac ggaagcacct
    16921 accctgcccg catttgaagc cttgtatttc atctgcggat ttattacgat attaccgtat
    16981 tcaggccgca ccgatgccgc ctgccccccg aaaaactttg gagaatccaa aaaatgtttc
    17041 attttgcatt tccggcacaa actgccctgc gccaagcgat aaccgatgcc taccgccgta
    17101 atgaaatcga agccgtacag gatatgttgc aacgtgcaca gatgagcgac gaagagcgca
    17161 acgccgcctc cgagcttgcc cgccgtttgg ttacccaagt ccgcgccggc cgcaccaaag
    17221 ccggcggcgt ggatgcgctg atgcacgagt tttcactctc cagcgaagaa ggcatcgcgc
    17281 tgatgtgtct ggcagaagcc ctgctgcgta tccccgacaa cgccacgcgc gaccgcctga
    17341 ttgccgacaa gatttcagac ggcaactgga aaagccattt gaacaacagc ccttccctct
    17401 tcgtcaatgc tgccgcctgg ggcctgctga ttaccggcaa actgaccgcc acaaacgaca
    17461 aacaaatgag ttccgcactc agccgcctga tcagcaaagg cggcgcaccg ctcatccgcc
    17521 aaggcgtaaa ttacgccatg cggcttctgg gcaaacagtt cgtaaccgga cagaccattg
    17581 aagaagccct gcaaaacggc aaagaacgcg aaaaaatggg ctaccgcttc tccttcgata
    17641 tgttgggcga agccgcctac acccaagccg atgccgaccg ctactaccgc gactatgtcg
    17701 aagccatcca cgccatcggc aaagatgcgg caggacaagg cgtttacgaa ggtaacggta
    17761 tttccgtcaa actttccgcc atccatccgc gctactcgcg cacccaacac ggccgcgtga
    17821 tgggcgaact gttgccgcgc ctgaaagagc tgttcctttt gggtaaaaaa tacgatatcg
    17881 gtatcaacat cgatgccgaa gaagccaacc gtctggagct gtctttggat ttgatggagg
    17941 ctttggtttc agaccctgac ttggctggct acaaaggtat cggtttcgtt gtccaagcct
    18001 accaaaaacg ttgtccgttc gttatcgact acctgatcga ccttgcccgc cgcaacaacc
    18061 aaaaactaat gatccgcctc gtcaaaggcg cgtattggga cagcgaaatc aaatgggcgc
    18121 aagtggacgg cttgaacggc tatccgacct acacccgcaa agtccacacc gacatctcct
    18181 acctcgcctg cgcgcgcaaa ctgctttccg cgcaagacgc ggtattcccg caatttgcca
    18241 cccacaacgc ctacactttg ggcgcaatct accaaatggg taaaggcaaa gattttgaac
    18301 accaatgcct gcacggtatg ggcgaaaccc tgtacgacca agtcgtcggc ccgcaaaact
    18361 taggccgccg cgtgcgcgtg tacgccccag tcggcacaca cgaaaccctg ctcgcctact
    18421 tggtgcgccg cctgttggaa aacggcgcga actcgtcttt cgtcaaccaa atcgtcgatg
    18481 aaaacatcag catcgacacg ctcatccgca gcccgttcga caccatcgcc gaacaaggca
    18541 tccacctgca caacgccctg ccgctgccgc gcgatttgta cggcaaatgc cgtctgaact
    18601 cgcaaggcgt ggacttgagc aacgaaaacg tattgcagca gcttcaagaa cagatgaaca
    18661 aagccgccgc gcaagacttc cacgccgcat ccatcgtcaa cggcaaagcc cgcgatgtcg
    18721 gcgaagcgca accgattaaa aaccctgccg accacgacga catcgtcggc acagtcagct
    18781 ttgccgatgc cgcgcttgcc caagaagcgg ttggcgcagc cgttgccgcg ttccccgaat
    18841 ggagtgcgac acctgccgcc gaacgcgccg cctgcctgcg ccgttttgcc gatttgctgg
    18901 agcagcacac cccagcactg atgatgcttg ccgtgcgcga agcaggcaaa acgctgaaca
    18961 acgccattgc cgaagtgcgc gaagccgtcg atttctgccg ctactacgca aacgaagccg
    19021 aacataccct gcctcaagac gcaaaagccg tcggcgcgat tgtcgccatc agcccgtgga
    19081 acttcccgct cgccatcttt accggcgaag tcgtttccgc attggcggca ggcaacaccg
    19141 tcatcgccaa acccgccgaa caaaccagcc tgattgccgg ttatgccgtt tccctcatgc
    19201 acgaagccgg catcccgact tccgccctgc aactcgtcct cggcgcaggc gacgtgggtg
    19261 cggcattgac caacgatgcc cgcatcggcg gcgtgatttt caccggctcg accgaagtgg
    19321 cgcgcctgat caacaaagcc cttgccaaac gcggcgacaa tcccgtcctg attgccgaaa
    19381 ccggcggaca aaacgccatg attgtcgatt ccaccgcact tgccgagcaa gtctgcgccg
    19441 acgtattgaa ctccgccttc gacagcgcgg gacaacgctg ctccgccctg cgcattttgt
    19501 gcgtccaaga agacgttgcc gaccgtatgc tcgacatgat caaaggcgct atggacgaac
    19561 tcgtcgtcgg caaaccgatt cagctcacta ccgatgtcgg ccccgtcatc gatgccgaag
    19621 cacagcaaaa cctgttgaac cacatcaaca aaatgaaagg tgttgccaag tcctaccacg
    19681 aagtcaaaac cgccgccgat gtcgattcca aaaaatccac gttcgttcgc cccatcctgt
    19741 ttgaattgaa caacctcaac gaactgcaac gcgaagtctt cggtcccgtc ctgcacgtcg
    19801 tccgctaccg tgccgacgaa ctcgacaacg tcatcgacca aatcaacagc aaaggctacg
    19861 ccctgaccca cggcgtacac agccgcatcg aaggcacggt acgccacatc cgcagccgca
    19921 tcgaagccgg caacgtttac gtcaaccgca acatcgtcgg cgcagtcgtc ggcgtacagc
    19981 ccttcggcgg acacggtctg tccggcacag gccccaaagc aggcggttcg ttctacctgc
    20041 aaaaactgac ccgcgccggc gaatgggttg ccccgaccct gagccaaatc ggacaggcgg
    20101 acgaagccgc actcaaacgc ctcgaagcac tggttcacaa actaccgttc aacgccgaag
    20161 agaaaaaagc cgcagcggcc gctttgggac acgcccgcat ccgcaccctg cgccgtgccg
    20221 aaaccgtcct taccggaccg accggcgagc gcaacagcat ctcatggcac gcgcccaaac
    20281 gcgtttggat acacggcggc agcacggttc aagcctttgc cgcactgacc gaacttgccg
    20341 cctccggcat acaggcagtg gtcgaacccg acagcccctt ggcttcctac actgccgact
    20401 tggaaggtct gctgctggtc aacggcaaac ccgaaaccgc cggcatcagc cacgttgccg
    20461 ccctgtcgcc tttggacagc gcgcgcaaac aggaacttgc cgcccacgac ggcgcactca
    20521 tccgcatcct cccttcggaa aacggactcg acatcctgca agtgtttgaa gaaatctctt
    20581 gcagcgtcaa caccacagcc gccggcggca acgccagcct gatggcggtc gccgactgat
    20641 tttgccgaaa tacccgggcg cggcccgtga accaatgccg tctgaaaacc tttcagacgg
    20701 catttttata
//
LOCUS       DAWGRZ010000017        19917 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000017 DAWGRZ010000000
VERSION     DAWGRZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19917
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.017"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(396..3002)
                     /gene="acnD"
                     /locus_tag="WHT44_000439"
     CDS             complement(396..3002)
                     /gene="acnD"
                     /locus_tag="WHT44_000439"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ2532668.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKDGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDSLGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELNGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYE
                     EPSDGQMPDGSVIIAAITSCTNTSNPRNVVAAALLARNANRLGLKRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRIHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSFARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPDTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCCLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            complement(3080..3868)
                     /locus_tag="WHT44_000440"
     CDS             complement(3080..3868)
                     /locus_tag="WHT44_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEZ2532669.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFVGGVAGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIAATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIVSMF"
     gene            complement(4021..5175)
                     /gene="prpC"
                     /locus_tag="WHT44_000441"
     CDS             complement(4021..5175)
                     /gene="prpC"
                     /locus_tag="WHT44_000441"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ2532670.1"
                     /translation="MTETTQTPTLKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            complement(5261..6136)
                     /gene="prpB"
                     /locus_tag="WHT44_000442"
     CDS             complement(5261..6136)
                     /gene="prpB"
                     /locus_tag="WHT44_000442"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ2532671.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            complement(6762..8072)
                     /locus_tag="WHT44_000443"
     CDS             complement(6762..8072)
                     /locus_tag="WHT44_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ2532672.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(8211..9389)
                     /gene="ftsZ"
                     /locus_tag="WHT44_000444"
     CDS             complement(8211..9389)
                     /gene="ftsZ"
                     /locus_tag="WHT44_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ2532673.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(9508..10752)
                     /gene="ftsA"
                     /locus_tag="WHT44_000445"
     CDS             complement(9508..10752)
                     /gene="ftsA"
                     /locus_tag="WHT44_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ2532674.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(10838..11566)
                     /locus_tag="WHT44_000446"
     CDS             complement(10838..11566)
                     /locus_tag="WHT44_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ2532675.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKTLGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            complement(11556..12470)
                     /locus_tag="WHT44_000447"
     CDS             complement(11556..12470)
                     /locus_tag="WHT44_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ2532676.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            complement(12583..13992)
                     /locus_tag="WHT44_000448"
     CDS             complement(12583..13992)
                     /locus_tag="WHT44_000448"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ2532677.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYLEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLALSKQI"
     gene            complement(14147..15214)
                     /gene="murG"
                     /locus_tag="WHT44_000449"
     CDS             complement(14147..15214)
                     /gene="murG"
                     /locus_tag="WHT44_000449"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ2532678.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPVTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGGLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(15218..16543)
                     /locus_tag="WHT44_000450"
     CDS             complement(15218..16543)
                     /locus_tag="WHT44_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ2532679.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGLLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(16669..18006)
                     /locus_tag="WHT44_000451"
     CDS             complement(16669..18006)
                     /locus_tag="WHT44_000451"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ2532680.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRDDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIVTQDIPLQGLHN
                     AANVMAAVALCEAIGLSREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALVGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLGEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(18090..18686)
                     /locus_tag="WHT44_000452"
     CDS             complement(18090..18686)
                     /locus_tag="WHT44_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ2532681.1"
                     /translation="MKQTVKWLAAALIALGLNRAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGRGVRRDDAEAVRWYRQAAEQGLAQAQYNLGWMYANGRGVRQDDTEAVR
                     WYRQAAAQGVVQAQYNLGVIYAEGRGVRQDDVEAVRWFRQAAAQGVAQAQNNLGVMYA
                     ERRGVRQDRALAQEWFGKACQNGDQDGCDNDQRLKAGY"
     gene            complement(18812..19894)
                     /locus_tag="WHT44_000453"
     CDS             complement(18812..19894)
                     /locus_tag="WHT44_000453"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ2532682.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIASLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
BASE COUNT         4734 a         5566 c         5011 g         4606 t
ORIGIN      
        1 cgccttgtcc cgatttttgt taatcctcta taccatgtca attcagcctt tatttcgcgg
       61 gcaaagccca cgctacaccc actttccaga agtacattag gctgccactc cgacaaagcc
      121 gtttgattcc gcaaactgtc ggggttcgcc acaatctacg gctactgtgt tgtgatacgg
      181 cagattatta aaccccgtca ttcccgcgca ggcgggaatc caaatttgtc cgcacagaaa
      241 ctcatcggat aaataaggtt tcctcaattc cactttctgg attcccgcct gcgcgggaat
      301 gacgatttac agtagatgcc tgccatatcg agaattacgt gaatgagcaa aatgttgaac
      361 ccgacccacg ctacgtcttg cttttcagac gacctctaag ccgcgttccc ttccaaaaaa
      421 tcctgtgcaa accgttgcaa cacgccgccg gcttcatata ccaatacttc ttctgcagta
      481 tcgaggcagc aggtaacggg aacttcaacg gtttcgccgt ttttacggtg aatcacgagg
      541 gtcaggtcgc agcgcggtgt gcgttcgccg accacgtcgt aggtttccgt accgtccagt
      601 tgcagggtat ggcggttggt gtcgggtttg aactgcagcg gcaacacgcc catgccgata
      661 aggttggtgc ggtggatacg ctcgaagcct tcggcaacaa tcgcttctac gccggcgagg
      721 cgtacgcctt ttgcagccca gtcgcggctt gagccttgac catagtccgc accggcaatg
      781 atgatgagcg gctgtttgcg gttcatatag gtttcgatgg cttcccacat gcgcatggtt
      841 tcgccttcgg gttcgacgcg ggcgaacgag ccttggcgca cgctgccgtc ttcgtttttc
      901 accatttcgt taaacagttt cggattggcg aaggtagcgc gttgggcggt caagtggtcg
      961 ccgcggtggg ttgcgtaaga gttgaagtct tcttcaggca aacccatttt cgccaaatac
     1021 tcgcctgcgg cactgacggc caaaatcgca ttggacggcg agaggtggtc ggtggtgatg
     1081 ttgtcgggca aaatcgccag cggacgcata cctcttaatg tgcgttcccc tgccagcgcg
     1141 ccttcccagt aaggcggacg gcggatgtag gtggacatcg gacgccaatc gtacagcgga
     1201 ctgggtgctt tttgcgctgt gccggtgtcg aacatcggta catacacatc gcggaactgc
     1261 tgcggtttca catattcggc aacgacggca tcgatttctt catcggcagg ccaaatgtct
     1321 ttcaggcgga tttccttgcc gtctgcaacg ccgagtacgt cgttttcaat atcgaaacgg
     1381 atactgcctg ccagcgcgta ggcaacgacc aacggaggcg aagcgaggaa agcctgtttc
     1441 gcatacgggt ggatacggcc gtcgaagttg cggttgcctg ataatacggc ggtggcgtac
     1501 aaatcgcggt cgatgatttc tttctggatt ttcggatcca gcgcgccact catgccgttg
     1561 caggtggtgc aggcgaaggc gacgataccg aagccgagtt tttccatttc gggcaacagg
     1621 cccgcttctt tcaaatagat ttcggctact tttgaacccg gggcaaacga agatttcacc
     1681 caaggtttgc gtttcaagcc gagacggttg gcattgcgtg ccaagagcgc ggcggcaaca
     1741 acgttgcgcg ggttggaagt gttggtgcaa ctggtaatcg cggcgatgat gaccgagccg
     1801 tcgggcattt ggccgtccga aggctcttcg taaggcttcg ccagcccttt cgccgccaaa
     1861 tcggcggtcg caaaacgggc atgcgggtta cttgggcctg ccatattgcg cgttacgctg
     1921 ctcaaatcaa atttcaaaac gcgaggataa acggcggttt tcaaggcatc tgcccacaag
     1981 cctgcggttt tggcgtaggt ttccaccaat ttcacctgcg cgtcgtcgcg tccggtcagt
     2041 ttcaaataat caatggtttg ctcatcaata gcgaacatcg cggcagtcgc gccgaactcc
     2101 ggcgtcatgt tggaaatggt cgcgcggtcg ccgatagaca ggcttctcgc gccctcgccg
     2161 aagaattcga caaacgcccc gaccacgcgt tctttgcgca gaaactcggt cagtgccaac
     2221 acaatatccg tcgccgtaat gcccgcctgc cgtttgccgt tcagctcaac gccgacaata
     2281 tcgggcaggc gcatcatgga cgcgcgtccc agcattacgg tttccgcttc caatccgccc
     2341 acgcccacgg aaatcacgcc caatgaatcg acgtgcggcg tatgtgagtc agtaccgacg
     2401 caggtatcgg ggaaagccac gccgtttttg acttggacga cgggcgacat tttttctaga
     2461 ttgatttggt gcatgatgcc gttgcccgcc ggaatcacgt ccacattttc aaacgcggtt
     2521 tttgtccagt tgatgaagtg gaaacggtct tcgttacggc ggtcttcgat ttcgcggttt
     2581 ttgcggaagg catcaggatc gtaaccgccg cactccaccg ccagagagtg gtcgacgatg
     2641 agctgggttt gcaccaccgg attcactttg gcaggatcgc cgtctttttc ggcaatcgca
     2701 tcgcgcaggc ctgccaaatc caccaacgcg gtctgcccca gaatatcgtg gcacaccacc
     2761 cgcgccggat accacggaaa gtcgatttcc tgcttccctt ctatcaactg ccccagccag
     2821 ctttgcagcg tcggcaaatc gactttgtcc gcgcggttga ccaaattctc cgccaaaatg
     2881 cggctcgtgt aaggcagctt gtcgtaagag ccgggcttga tgtcctcaca cgccgcacgc
     2941 gcgtcgtagt attccaaatc cgtaccgggc agcggtttgc ggtaacgttg gttggcagcc
     3001 atgtcggttc tcctgtggat ctgtttttct tgtggtttga ggtttcagcc gacgttttga
     3061 aggggtcgtc tgaaagggtt taaaacatcg aaacaatcat ctgatacagc ggatttctct
     3121 cgtctatcaa caatttcaca gccatcgaaa tgctgatgac aatcagcagc ggcttaatca
     3181 gcttcgaacc gaagcggacg gcaaatctcg cacctaaatt cgcaccgaca aacgcaccga
     3241 ccgccatcgt tgccgcaatc gggaaaataa tcgaaccgtg cagcaggaat accgatagcg
     3301 aaccaagatt gcaggcaacg ttcgccaatt tggtgtaaga catcgcgttc aacagcttgc
     3361 agccgagcaa aacaataaag gcaatcagaa aaaacgagcc gacacccggt ccgaacacac
     3421 cgtcgtaaaa acccaaaagc ggtgcgaccg tcagcccgaa cagaaaaaaa gacattctgg
     3481 ctttgccttc cttactgccg tcgagcttgg gcgaaaacac aaaatacagt gcgacaaata
     3541 tcaacaaaac cggcacgacc gccagcagaa tatctttgga aaccaagctg accgataatg
     3601 caccggccac gccgcctaca aacgatgctg cggcaatcgg gagacctttc ttccaatcaa
     3661 tcaaaccttt gcgtgcaaaa gaaaccgtag ctgaaaacgt agcagcggct gcttgcagct
     3721 tgttggtggc aattgccgac acgggaggaa tacctgccaa caagagtgcg ggcagcgtaa
     3781 tcaaaccacc cccgcccgca atcgcatcga taaatccggc aatcatcgca accaaaccca
     3841 aagcgagtat tatatataaa tcttccatgt ttcttatcct gttacttgcg ccaatacagg
     3901 attatcttct cctattagat taaacttatt tcagacaacc tttccaataa ggcaaggtcg
     3961 tctgaaatcc ttagctttgc ataccgaaat taaagaacaa actgctattg cattcttcaa
     4021 ttatcgttct tcaatctcca caaacgccaa atcttcaggg cctgtgtagt ttgcgctcgg
     4081 acggatgatt ttgccgtctt tgcgttgctc aagaacgtgt gcgctccaac cggttgtacg
     4141 ggaaattacg aacagcggtg tgaacatagc ggtcggtacg cccaattttt ggtaggaaac
     4201 ggcagagaac cagtccagat tcgggaacat ttttttctct tcccacatca cgctttccaa
     4261 acgttcggca atgtcaaaga ggcgcatatc gccggtttct ttgctcaaac cgcgtgccac
     4321 ttctttaatg acaacgttgc gagggtcgga aatggtgtac accggatgac cgaaaccgat
     4381 cacgatttct ttgcggccga tgcgttcgcg gatgtcggct tcagcttcgt cggcattgcg
     4441 gtagcgtttt tgaatatcgt aagccacttc gttcgcgccg ccgtgtttcg gacctttcaa
     4501 cgcgccgatt gctccggtaa tgctggagta catatcagag cctgtaccgg cgatcacgcg
     4561 ggcggtaaag gtagaagcgt tgaactcgtg ttcggcatac agaatcagtg aaacgtgcat
     4621 ggctttgatg tgtgattcgc ttgggcgttt gccgtgcaac agttgcagga aatgaccgcc
     4681 gatggtctct tcgtcgcttt caacctcaat gcgtttgccg ttgtgcgaat attgatacca
     4741 gtacaagagg atgctgccga ggctggcgat cagtttgtcg gcgatgtcgc gcgcttcact
     4801 ttccggatgg ctttcacgtt caggatgaac gcagcccagc atggatacgc cggtacgcat
     4861 tacgtccatc ggatgggtat gtgcaggcag gctttccaaa actttaatca cacggatagg
     4921 caggccgcgc atggatttga gcttggtttt ataagcggcc agctcgaatt tgttgggcag
     4981 atggccgtga atcagcaggt gggcgacttc ttcaaactcg catttttgtg ccaaatccag
     5041 aatgtcgtaa ccgcgatagc tcaaatcgtt gccggtacgg ccaacggtac acaaagcggt
     5101 attaccggcc gcaacgccag aaagcgcaac ggatttttta ggtttgaggg tcggggtttg
     5161 agtagtttca gtcatggtat ttctcctttg tgtttttatg ggtttcgggt tttcagacga
     5221 ccgatgcgga tttgttgaaa ggcagtctga aagcggtaaa tcatttttga aacaatttat
     5281 ccagtttttg ctcgaaggca tgatagttca gatgctcgta cagctcggca cgggtttgca
     5341 tactgtccac caccgccgcc tgagtgccat cgcgcataat cgcttcgtaa acattcagag
     5401 cggctttgct tgctgcacgg aacgatgaca gcggatacag caccagcgac acgccgtttt
     5461 cagccagctc gctttgggta taaagcggag tggaaccaaa ctcggtaatg ttcgccaaca
     5521 cgggcacttt caccgcatct gcaaattggc ggtacatgtt caaatcggtc atggcttcag
     5581 ggaaaatcat gtccgcaccg gcttcgacac aagcttgggc gcgttcgata gcggcatcca
     5641 aaccttctac cgccagcgca tcggtacgcg ccataatcac gaagttctca tcaacgcgcg
     5701 catctacggc agctttgata cggtcgacca tttcatcttt agatacaatg gctttgttcg
     5761 gacggtggcc gcagcgtttt tgcgctacct gatcttcgat gtgaaccgct gcaacaccgg
     5821 cgcgttcaaa gttgcgaatg gtacgggcaa tattgaatgc accgccccaa cccacatcga
     5881 tgtccaccag cagaggcgta tccacgttgt ccgtaatgcg tcgtgcgtcg atcagcacat
     5941 cttccattgt ggtaatgccc aaatcaggga taccgcaaga acaggctgcc acgccgccgc
     6001 cggacagata gatggctttg aaaccgcttt gggtggccaa tcgtgcaaaa taagcattga
     6061 cgcaaccggc gacggcaagc ggattcgatt ctttcacggc ttggcggaaa cgtgctccgg
     6121 cagagtgttg actcatcata tttctccttt atagactttt tttcagtatg gacaggcttc
     6181 catcacattc ggacggcaaa acacagccat ccgacgcgtc gggcatccca atccaatcat
     6241 taaaaatata tgggaaaaat tatcttattg atatttaaaa cgaatcaaag aaaaacagca
     6301 gaccgttcgg aattatgcgg caaaaccgca gacaagaaga aaacaagggg attattcaga
     6361 aaaggggaaa acatctgaat tggtttcata gtaatgttcc tttgtagtgt tatgtagttt
     6421 tatttttcga taatctaaaa cacaagactg aaaatgtcaa acatttttaa ggagactcat
     6481 tacataattt taatatttta ttttaattcc atgattaaac tagattaatt ttgaattatt
     6541 tgtttcaata aaattgtcag gaaatcaaga aaaatttttg ataatcaaaa tcctgaaaac
     6601 ttataaaata ttttatacat aaaaacaatg tattattttt aagcaatccg atgtatttta
     6661 ttatgtattt gatttttata taatttaata aaaattaaat atatcggcaa acaaaatgcc
     6721 gtctgaaaac ctttcagacg gcatttttaa tatcgaatca atcagcccaa ataccagaat
     6781 ttcagtgccc acaatacggc aacaatccat accataggcg gaacgtcttt ggtgcggcgg
     6841 cataaaagtt taaccacggc ataactgatg aagccgaaag cgatgccgtc tgcaatcgaa
     6901 taagtaaacg gcatgaaaac aatggtcagg aacgcaggtg cggcttccgt catatcgtcc
     6961 caatcaatat ccctcgcact gcggagcatc tgcgtgccga cataaagcag ggcgggcgcg
     7021 gtggcaaaag cgggaacact tttcgccaaa ggtgaaaaca tcaggcaggc gagcatcaat
     7081 acgccgacgg taaccgccgt caggccggtc cgtccgcctg ccgatacgcc cgccgcgctt
     7141 tccacataag gcgtggtgga agaagtaccc aaagccgcac ctgccacaat ggcggtagag
     7201 tctgcaagca gtgcgcgttt caggcggggc agcttaccgt ccaccagcag cccggcacgg
     7261 tgggatatgc cgaccagcgt tccggtactg tcaaatagat cgaccaagaa gaagacgaaa
     7321 atcacactga ccatgctgac ggtaaacagg ccttcaaaat ccatctgcat aaaagtcggc
     7381 gcaatgctcg gtacttcgcc gatgatgccg tgaaattcat tcaaacccat caggctggca
     7441 atgacggtaa tggtcaagat ggtgatgatg attgcgcctt gaacgcggaa atgtcccaat
     7501 acgaccacca tagcaaaacc gaacaatgcc aacaacgcgg acggctgatg aatatcgccc
     7561 aaaccgacca aggttgccgg attggcaacg ataatgcctg cgcctttcag ggaaatcagt
     7621 gccaaaaaca aaccgatacc ggcagcaatc gacattttca aacccatagg cagtgcgttg
     7681 accagcattt ccctgacttt aaaaaagctg aacaggataa aaatcagacc ggagatgaac
     7741 accgcaccca acgcaacctg ccaaggcacg cccataccct taacgacggc aaaggtgaaa
     7801 taggcattca gccccatccc cggtgcgagt gcaatcggat agttgccgac aaaacccata
     7861 acaaaacagc cgatggcaga cgcgatacag gtagcgacga ataccgcccc catatccatg
     7921 ccggtctcgc ccaaaatcag agggttgacg ataacgatgt agcacatcgt caaaaaagtt
     7981 gtcaaacccg ccatcaactc ggtacgcacc gtcgtaccgt ttgccttcag cttaaaaatc
     8041 ccgtccaaca gtgtttgttt tgaagtgtcc atatctgaac gtctgtatca agtggaaaaa
     8101 tctgaatccg ccgaccgcaa accgtacgaa attgcaacat cttgaaaaac atacctccca
     8161 aaccggacaa accgattcgg gaggcagcag gtttacgggc aaacagcaca ttatttgtct
     8221 gaattgtgtt gacgacgcaa aatcgcaggg atttcaaagt cgtcaagtac ggactgattg
     8281 tcgaaatccg cagcggtaag gttcatcgtg cggataccgc gattggtgcg gatcatacct
     8341 tcgacattgt ggctttgctc ctgtttggac ggagcaaccg cttctacctc ccttgccgga
     8401 acaaaatcga ccgcgccttt ttctttcaga ccggtagcga taatggtaat ccggatggca
     8461 tcttcgctca tggtctcgtc ttcagccgca ccgaatttgc attccaaatc gggatgcgcg
     8521 ctttggttga cgattttcat gacttcggac aactcggaca ttttcaagca acccggagca
     8581 gtcgtaatat tgaccagcac accgcgcgct ccgtccaagg ttacatcgtc cagcagcgga
     8641 ctggaaatgg cctggtcggt cgccatacgc gcacggtcga taccttgggc ataacccgaa
     8701 cccatcatag cgataccgcg gttgctcatc acggttttca cgtcggcaaa gtcgaggttg
     8761 atgatttcgc tcgggcaagt taccacttcg gaaatgcctg cgaccgcatc gcgcaataca
     8821 ttgtcggcgg cacggaaggc ttcgcgcatc gttacgtctt cacccaatgc agtcatcagt
     8881 ttgtcgttcg ggatgataat cagcgaatcg acgtgttctt tcaactgttc caaccctgcc
     8941 tgtgcgacat ggacgcgctt accttcatat gcgaacggtc gggtaaccac ggcaacggtc
     9001 agaatgccca aagacttggc aatctcagca acaaccggcg cggaaccggt accggtaccg
     9061 ccgcccatac cggtcgtgat aaacagcata ttcgcaccgc gaatggcttc ttcaatggct
     9121 tcccggtctt cctgggctgc cgcacggccg atatcgggat tcgcgcccgc gcccaaaccg
     9181 cgtgtcagat tcgtacccaa ctggattctc ttcgccgcat ggttttttgc cagagactgc
     9241 gcatccgtat tggcactgat aaactccaca ccgcgcacat tgttggcaac catgttattg
     9301 attgcattgc aaccgccgcc gcccaagccg attactttaa tcaccgcagg gctgactgcc
     9361 gattctgcca cgtcgtaaac aaattccatt caaaaactcc tgctcgcccc attcagagga
     9421 cggtttaaat aaattattat tcattatata agaagtatct tgctgctggc aaaatacttc
     9481 tcacctgtca aacggcaatc cacctgttca gaagctgttt tcaatccacc gtttcaatct
     9541 tgccaacaaa ccgccgcccc cttccctctc ttgcactgca ccgttttccg gctgcggcaa
     9601 gtttccttcc agcttgcatg ctgcatgaag cagcccgata gcggtagaaa aacgcggtgt
     9661 gcggacgcgg tcggacaaac cgcccatttc ttggggtgca ccggtgcgta caggcaaatc
     9721 gaagattttt tcggcaaatt ccacaatccc ggtcatcatg gacacaccgc cggtcagaac
     9781 gatacccgca ttcagcactt ctttggggaa acccgatttt tgcagctcgc ccagcactac
     9841 gccaaaaatc tcctgaatcc gtgcactgat gattgctgcc agaaccttac tggaaacctg
     9901 acgcgatgtc cggtcaccca cgcccggaac ttcaatcatc tcacccaagc cttccgtatc
     9961 gcatgatgcc acgccataat ggattttaat gtactcggcg gcatcgagag gtgttctcaa
    10021 cgatttggac aaatctttgg taatcagatt accaccggcc ggaatgacgg acgtatggcg
    10081 gatggcaccg ttcatataaa cggcaatatc ggtcgttccg ccaccaatgt cgatgacgca
    10141 tacgccgagg tctttttcat cttcagtcag caccgcctgc ccgcttgcca acggctgaag
    10201 catgatctga tcgcttttca aaccgcaccg ctcgatacat ttttggacat tctgcactgc
    10261 cgtacttgca ccggtaatga tgtgcacccg cgtatccaga cgcacaccgc tcataccgat
    10321 gggctccctc acgccaagtt gggtgtcaat aatgtagtct tgaaccacgg catcgagaat
    10381 tttttgatcg ggcgggatat tgattgcctt tgccgtttca atggcgcgat cgatgtctgc
    10441 ctgcgtgact tccccatctt taattttaac cacaccttgc gaattgagac tgcggatgtg
    10501 gttgcctgcg atacctgtgg taacgtgagt aattttggta tccgccatca gctcggcatc
    10561 attgaccgcc tgcctgatgg cttggacggt ggcatcgata ttggttacca tgcccgcgcg
    10621 caagccccgt gaaggagcct gccccaaacc gacgatgttg attttgtcgt catcttgaac
    10681 ttccccgatc agtgcgagga ctttagacgt accgatatcc agtacgctga tgtatctttg
    10741 ctgctgttcc attgttcgtc tgctcttaaa actgattgaa atttgcgtcg caccgtttca
    10801 gacggcacgg ccgtaatctg tccgatacct gttcccacta ttcttcggat tctttttcgg
    10861 gtaaaccgtc ggaagcatag cggactgaaa atccgtcctt atacctcata tccacatagg
    10921 ataaccgatt tttattttta cgcaacagat gctgccacgc ttcggtaaaa agccggaggc
    10981 gtttcatctc gttttcccgt ccgagcctga cggtgatgcc gttgtccaaa acgacaatcc
    11041 acgccgaacg tgccgtatag gtcatctctt tgatgcccaa accctgtttt gccaaaacag
    11101 tcgaaaattc gtcataacgg cggagcattt cggcagacgt tccttccgcg cctctgaata
    11161 ccggcattcc gggtctgtcc aagcgggctt caaaaacatt gccttcgccg tccaccaagg
    11221 catggtcgcc ccaacgcgcg accggcttgc gctcggtcag gacgacctca accgtgtcgg
    11281 gaaaacggcg gcgcaccatg accgacgcaa tccacggata ccggcggtag gcctcctgtg
    11341 cgccattgat gtccgtcctc aaaatattcc catggatgta ttctttcgcc aaactgccca
    11401 atgtcttctt atcggaataa accaggttgc ccttcagcga cacctgcttg acgggcagat
    11461 gattcgaatt gtaaaaccaa accagcccgg acgcagcaag cagcatcgcc atcatgacaa
    11521 gcagccagcg cgtcagccgt tccatcgctt cggcattatc ccacatgtgc ggtcttcaaa
    11581 atttcaatac ataaatcggc aaaacccacg cccgtaacgg cagcggattt cggtactaaa
    11641 ctatggctcg tcataccggg cagggtgttg atttccaaca gatagagttt gccgtcggta
    11701 tctttgagga aatcgacgcg cacgcagcct tccgcaccga ttgcctgcgc gccgcgaacc
    11761 gccagttcgc gcatcaggct ttcttcggct tcggtcaaat cttccgaagg acattgataa
    11821 atggtgtcgt cgcggttgta cttggcttcg tagtcgtaaa actcggttgc gggaatgatg
    11881 tgtatgccgg gcagcccttt gccgttcagg acggggcagg aatattcgcc gccgccgata
    11941 aaacgttcgg caatgatttc gccctgaagg tgtttcaatt cttcgtaaac gcttttcaga
    12001 cggccttttc ctttgacttt taccacgcct acgctgctgc cttcggccgc cggtttcaca
    12061 aacatcggca ggcccaattt ttcttcgacg gcatcgaaat cagtgtcgtc gtgcaggacg
    12121 gcgaactcgg gaacgggcaa tcccaatgcc tgccaaatca gtttgcagcg gtatttgtcc
    12181 atgccgatgg cggatgcggc gacaccgctg ccggtatagg gaatgcccaa cagttccaat
    12241 gcaccctgaa ccgccccgtc ttcgccgtaa gtaccgtgaa ggatgttgaa tgccgtctga
    12301 aaaccttgtg ccttcaattc agacaatggg gtttctttag gatcgaaggc gtatgcgtct
    12361 atgcctttgc tttttaaagc attcaaaatg gcggtgccgc tgtccagcga gatttctcgt
    12421 tcgctggaaa aaccgcccat caatacggcc actttgccaa aattctgcat tgtttttgtt
    12481 ctttcctgat tgctttatgc ttgttgccag aggtcgtctg aaacctgatt tgcggtttca
    12541 gacgatcttt atatgatgtt ccgtctgtca ggcgggtgtg cctcaaatct gtttcgacaa
    12601 tgccagcagc gcggcgggga cgcggttgat gcttcccgcg cccatattca acacgatgtc
    12661 gccgtcctgc aaaacgttca acagcatttc gggcagatcg gcaacgtttt cgcagtaaat
    12721 cggctcgagt ttgcccaaca cgcggatggc gcgggcaaga gcgcgggaat cggcggcggc
    12781 aatcggctct tcaccggcgg cataaacttc ggtcagcacc agcgcgtcaa cggtattgag
    12841 gactttggta aagtcttcaa acaaatcgcg cgtgcgggta tagcggtgcg gctggaaggc
    12901 gagtaccaaa cgtttttcca gatacgcgcc gcgtgcggcg gcaagggtcg ccgccatttc
    12961 gacggggtgg tgtccgtagt cgtccaccaa gagcgcggtc ccgccgtttg gcaacttgat
    13021 gtcgccgtat ttttggaagc ggcggccgac gccttcaaag ccgagcaagc ctttttggat
    13081 cgcttcaacc gatgcgccga cttccagcgc cacgccgatg gctgccaatg cgttcagcac
    13141 gttgtgtctg ccgggcatat tcagcacgac ttcaaacgac ccctgctcat gtcctttcat
    13201 ttgaacatgg acggtgaatt tcatttgcgc gccgacgttt tcgatgtcgg tggcgtagat
    13261 gtcggcggta tcgtccaaac cgtaagtagc ataaggtttg ctcactttgg gcaaaatcgc
    13321 gcggacgtgt tcgctgtcaa tacacaaaaa ggctttgccg tagaagggca tacggtggat
    13381 gaaatcgata aacgcctgat gcagtttttc gacgctgtgc ccgtaggtat ccatatggtc
    13441 ttcgtcgata ttggtaacga cggacataat cggtgtcagg tgcagaaagg atgcatccga
    13501 ctcgtcggct tcggcaacga tgtattcgcc tttgcccaag cgggcgttgg tgcctgcggc
    13561 gttgagtttg ccgccgataa cgaaagtcgg gtcaagtcct gccgcgccga ggatggaggc
    13621 ggtcaggctg gtggtcgtgg ttttgccgtg cgtgccggca atggcgatgc cgtcacggaa
    13681 gcgcatcaac tccgccaaca tcagggcgcg cggaataacg ggaatttgct gctccaacgc
    13741 agcgacaact tcgggatttt cttttttgac ggcggtagag gtaacgacga catccgcacc
    13801 gttaacgtgt tcggcggtat ggccgggata aacttgaatg cccaggctgc ccaaatgctc
    13861 ggtagcggca tttcgcgcct gatccgaacc ggaaacttta aagcccaaat tgtgcaagac
    13921 ttcggcgatg ccgctcatgc cgacgccgcc gataccgaca aaatggatgt tggtaactcg
    13981 atttttcatc ataatgttgc gttccggtgg attttcgatg cgtaaaggcg ttattttaaa
    14041 gggctgaccg tttgcgcgcc atagttttct gacaaatata tagcggattg aaataaaaaa
    14101 catccatgcc gtctgaacgg cttttcagac ggcatggttc ggcagtttac gccgcacacg
    14161 caatcgcggc ttccgccacg tcgtccgcac tgtgcggcag tgccaacgta cgggcgtttt
    14221 ctgcccattt gaggcatttt tcgcggttta agccgccgag aatctcggcg agtttttccg
    14281 ccgtcaactg ggtttgcggc aacagcaatc ccgcctccgc ctgcaccata aaacgcgcgt
    14341 tggcggtttg gtgatcgtca accgcgtgag gatacggcac taacaacgca cccaatcccg
    14401 ccgccgtcaa ctcggcaatc gtcagcgcgc cggcacggca aatcaccaaa tcggcatcgc
    14461 ggtaggcgga caccatgtcg gtaataaatt ccacgcattc ggctttcacg cccagcgcgt
    14521 cgtaatccgc ctgcaagctg cccagcttgc cccgtcccga ttggtggtac atctgcggac
    14581 gcgcattgtc gggcagcaaa gccaatgcct gcggtacggt tttgttcaaa acgtccgcgc
    14641 ccaaactgcc gccgaccacc aaaattttca gacggccttc acgcccttgg aagcgttcgg
    14701 caggcacggg caggttgcta atatcggcgc ggacggggtt gccgaccaag ccgccttcgt
    14761 ggctgaacgc tttcggaaaa gcgtacaaca cccgcttcgc ccagcgcgac aggtggcggt
    14821 tggacaaacc tgccacggcg ttttgctcgt gaatcacaat cggcacgcct aatagcttcg
    14881 ccgccaaacc gccggggaag gtaacgaagc cgccgaagcc gatgacgcac tcgacacggt
    14941 gtttgcggat aatccgctgc gcttcgcgga cggtttgata caaagtaacc ggcagcatca
    15001 gtttgcgttt gatgccgttg ccgcgcacgc ctttaatcgc cagcgtttcc aagcgtatgc
    15061 cgtattgcgg cacgatacgc tcttccatcg aatccttgct gcccagccaa atcacatgat
    15121 ggccgcgcgc gcgcaatgaa tccgccaccg ccagcgcggg gaaaatatgt ccgcccgttc
    15181 cgcccgccat cagcataaag gttttaccgc ccatgattta ctccacccga taaccgcgca
    15241 ttttccggcg gttttcataa tctatacgca acagcagcat catgctgatc agcatgaaaa
    15301 agactgacga accgccatag gacatcaacg gcagcgtcag acctttggtc ggcaaagcac
    15361 cgatgttcac accgatattg aagaaacttt ggataccgat ccaaatgccg atacccgaag
    15421 cgatataggc gttgaaagtc aaacccaaat cgcgcgactg cttgccgatg gaaaacgccc
    15481 gcaccaccag ccagccgtaa cagaatatca gcacgcacat accgaagaaa ccgaattctt
    15541 cggcgatgat ggcaaaaata aaatcggtat gcgcttccgg cagaaagccg cgtttgctca
    15601 aactcgcacc caaacccata ccgaaccact ctccgcgccc gattgccatc agagagtggg
    15661 taagctggta gccggcaccc tgcgggtctt tccacgggtc caaaaatgcc actacccgct
    15721 gcacacggta gggagcggcg gtaatcatca gcaccatccc gcccaagacg ctgcctacca
    15781 ggacgaaaaa atatttccac ggcaatcctg ccaaaaacag cattccaacg gcaatgacgg
    15841 taatgacgac aaacgaaccg aaatccggct gtaccattat cagcaccaaa ccgaacgcca
    15901 ccagcataat cggcaggatg atcgcccgga aacggccgta catttctaat gtttcacgac
    15961 gtgcctgcgg attggtggcg gacatgatca gattggccgt cccccgccaa atcgactgcc
    16021 aacccaaact ttccatgctg cgcaacactt cttcacggcg cgtgaacagg cttgccaaat
    16081 aaaggatgac cgccagcttg aacagctcgg tcggctggaa attcaacgga cccaaaggta
    16141 tccaacgggt cgcgccattg atttcgcgcc cggcaatcaa tacgactacc agcaacaggc
    16201 cggatagggc aaaaatccac ggcacaagcc gccgccatgt cctcatcctg caaagaaacc
    16261 ataacaaacc gctcgctatc aagccggcaa cgacgaaccc cgcctgtctg gtcaaataga
    16321 aaaactgatc gccgccttct tttgatgcca aatacacaga agccgaataa atcatcagca
    16381 ggctgaacgc cgtcatcagc accaccatcc acaaaagcgg cgcgtcgaat ttcctgccgt
    16441 cgcgcacaat cggcctgtcg agcagcagag tgtggacacc gtcgcccact tttaccaata
    16501 cttccgaaat cttcaaaaaa accacctgcc agtctgtttg cacctgccgc aaagggcaaa
    16561 aatttcagac ggcagacaat gccgtccgaa catacgatac atcccaaatc ggtattctaa
    16621 atctttactt gccgcccaac aatgacggcg tttgcatttc agacggcatc acaaagcctt
    16681 aaacgcttcg ataaacactt ccgaacggtg cgcgtagcct ttgaacatat caaagctcgc
    16741 gcaggcgggg ctgagcaaca caatatcgcc tgcttcggct tgggcatatg ccgtctgaac
    16801 ggcttctccc aaagtggcgc agtcggtcat attcaagccg cagccgtcca aatcgcggcg
    16861 gatttgcggc gcatcgacac caatcaagaa cacgcctttt gccttgccta ccagtgcatc
    16921 gcgcaggggc gtgaagtcct gccctttacc catgccgccc aaaatcacga agagcggatt
    16981 ttgcaaaccg gcaatcgcgg cggcagtcgc gccgacattc gtgcctttgc tgtcgtcgat
    17041 aaacaccacg ccgtttttct cgccgatttt ttccacgcgg tgcggcaggc cttggaaggt
    17101 tttgacgtgt tcgagcaatg cttcgcgcga caaaccgatg gcctcacaca aagccacggc
    17161 agccatgacg ttagcggcgt tgtgcagacc ttgcaacgga atgtcttgcg tgacaatcaa
    17221 atcttcattg ccttgtttca ggcggcctgt ctcgcgttcc aaccagaaat cagcttcgtg
    17281 ttccaacgaa aaccatttta cctcgcgccc ggcacgcttc atcgcgcggc agaacgcatc
    17341 gtccgcattc aaaacctgca cgccgtcgcc acggaaaatc ttggctttgg tatgcgcata
    17401 gtcgagcaag tcgtcgtagc ggtcgagatg gtcttcggaa atgttcagca ccgtcgccgc
    17461 agtcggacgc aggctttcgg tgttttccag ttggaagctg gaaagctcca acacccacac
    17521 gtccgccttt ttgccttcgc gctgccattc cgcctccaaa accggcgtgc cgatattgcc
    17581 cgcgataacg gtatccagcc cgcacttgat acagagatag ccgaccaggc tcgttaccgt
    17641 ggttttgccg ttgctgccgg taatcgcaat taccttgtcg tcccggcggt tcacaatgtc
    17701 cgccagcaat tcgatgtcgc ccaacacgcg tccgccgttt tgcttgaacg cctcaatatc
    17761 cggctgccgc tcgctgatgc cgggactgag agccagaata tcgaaaccgt tgtccagcgc
    17821 atctttcaga cggcccgtgt aaaacaccaa cccgtcaaac atcttaccga tttgcgacac
    17881 gcgttccggc ttcagctccg catcatacgc agcaacctcc gcgccgtttt tgcgcaggta
    17941 ggcaatcatg gaaatacccg taccgccgag tccggcgacg aggatttttt tgttttgaaa
    18001 agtcattttg gtttgtccta aaacaaatca tattgagcag gagatgtccg cccctgccca
    18061 agccgctttc agacggcatc gcgagctgtt caataacccg ccttcaggcg ttggtcattg
    18121 tcgcagccgt cttggtctcc gttttgacaa gccttgccaa accattcttg tgcaagggcg
    18181 cggtcttggc gcacgccgcg tctttcggca tacatcacgc ccaaattgtt ttgggcttgg
    18241 gctaccccct gcgctgccgc ctgccgaaac catctgaccg cttcgacatc gtcttggcgc
    18301 actccacgtc cttcggcata tatcacgccc aaattgtatt gggcttggac aaccccctgc
    18361 gctgccgcct gccgatacca tctgaccgct tcggtatcat cttggcgcac gccgcgcccg
    18421 ttggcataca tccagcccaa attgtattgg gcttgggcta acccctgttc cgccgcctgc
    18481 cgataccatc tgaccgcttc agcatcatcc cggcgcacgc cgcgtccttt gtaatacatt
    18541 gcgcccaaat tgtattgggc tgctgcattt ccctgtgctg ccgcctgcaa gttttcccga
    18601 aaatccgata cgtcatccgc ccacaccgct cggttcaagc ccaaggcaat cagggcggcg
    18661 gcaagccatt tgactgtctg tttcatggtt ttacttctgt tttagtataa ggcgggtttc
    18721 agccaccgtt aacgataggg ctgggcggat tgtcgccgca ggtttattgc gcgttcaaat
    18781 gccgtctgaa agatgttcag acggcatagg ttcagcggat tttgagggta ctcaaaccga
    18841 tcaacaccaa gacgatggta ataatccaaa agcggacgac gacttgggtt tctttccagc
    18901 ctttttgttc gtagtggtga tggatgggcg ccatcaggaa gatgcgtttt ttggttttct
    18961 tataccagcc aacctgaagc ataacggata cggcttctac gacaaataat ccgcccataa
    19021 tgacgaggac aaactcttgg cggacgataa cggcgacggt accgagcgcg gcacccaatg
    19081 ccaatgcacc gacatcgccc ataaagactt gcgcgggata ggcgttaaac cacaagaaac
    19141 cgaggcacgc gccgcacatg gcggtacaga aaatcaccac ttcgtttgcg ccggcaacgt
    19201 aaggtaattg caggtattgg gcaaattgtg agtggccgct ggcataggcg aagatggcga
    19261 ggccggcggc aacgaggacg acggggaagg tcgcaaggcc gtccaagccg tcggtgaggt
    19321 tgacggcatt ggatgtgccg acgatggtca ggtaagacaa caccaaaaag ccgaccacgc
    19381 ccagcggcag ggcgatttgt ttgaagaacg ggacaatcaa aatattgttg gcggaattgg
    19441 cggcaaggta aaacaatgcc aaactggcga taatggcaac gcttgactgc cacaccattt
    19501 tgaatttggc ggacacgccg ttggggtctt tatagacgac tttgcgccag tcgtcgtaaa
    19561 aaccgagtgc gcccgtggcg agcaatacgc ccaagagaat ccagatatac gggtttgccc
    19621 agttgcccca caacagggtg gacacggtaa tggcggtcag aatcagcgaa ccgcccatcg
    19681 tcggcgtgcc gtttttgacg aggtgggttt gcggaccgtc ggtacgcact gcctgcccgc
    19741 atttgagcgc ggtcagcctg cgtatcgtcc acgggccgaa catcagggaa aacgctaagg
    19801 cggtcaacgc cgccatgacg gcgcggaatg tggtgtattg aaaaatattc agaccggtta
    19861 accagttgct gaaatgtgcg agccataaaa acatggggct tccttttttt gttttgt
//
LOCUS       DAWGRZ010000018        19792 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000018 DAWGRZ010000000
VERSION     DAWGRZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19792
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.018"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(203..278)
                     /locus_tag="WHT44_000454"
     tRNA            complement(203..278)
                     /locus_tag="WHT44_000454"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Asn,seq:gtt)
     gene            complement(371..730)
                     /locus_tag="WHT44_000455"
     CDS             complement(371..730)
                     /locus_tag="WHT44_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ2532683.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(753..1397)
                     /locus_tag="WHT44_000456"
     CDS             complement(753..1397)
                     /locus_tag="WHT44_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ2532684.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1399..1935)
                     /locus_tag="WHT44_000457"
     CDS             complement(1399..1935)
                     /locus_tag="WHT44_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ2532685.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1947..3044)
                     /locus_tag="WHT44_000458"
     CDS             complement(1947..3044)
                     /locus_tag="WHT44_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ2532686.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3047..4483)
                     /locus_tag="WHT44_000459"
     CDS             complement(3047..4483)
                     /locus_tag="WHT44_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ2532687.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLILSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="WHT44_000460"
     CDS             complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="WHT44_000460"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ2532688.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMTHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCNVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFITHCKTGLKTNGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQAWLAKDFELLWCEDFTVILDFDTPSDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFGMPSGKVRLTYHPLFFIARYSAAGRQ"
     gene            complement(5473..6120)
                     /locus_tag="WHT44_000461"
     CDS             complement(5473..6120)
                     /locus_tag="WHT44_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ2532689.1"
                     /translation="MKTKFYNHQGGHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCT
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFGEIHQELIALFAMIGQDRR
                     TDLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDVGHYLFSRFTHWSALWNH"
     gene            complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="WHT44_000462"
     CDS             complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="WHT44_000462"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ2532690.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPSTVPKNTSRIRLSLTADMTTDEVRQFAACL"
     gene            complement(7294..7431)
                     /locus_tag="WHT44_000463"
     CDS             complement(7294..7431)
                     /locus_tag="WHT44_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HEZ2532691.1"
                     /translation="MATETNVAEISALDYEGRGVAKVGGKTVFIKRALLDCLMLGWFR
                     L"
     gene            7495..8571
                     /locus_tag="WHT44_000464"
     CDS             7495..8571
                     /locus_tag="WHT44_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ2532692.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTIIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(8778..10244)
                     /locus_tag="WHT44_000465"
     CDS             complement(8778..10244)
                     /locus_tag="WHT44_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ2532693.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10442..11365)
                     /gene="speB"
                     /locus_tag="WHT44_000466"
     CDS             complement(10442..11365)
                     /gene="speB"
                     /locus_tag="WHT44_000466"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ2532694.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11464..13356)
                     /gene="speA"
                     /locus_tag="WHT44_000467"
     CDS             complement(11464..13356)
                     /gene="speA"
                     /locus_tag="WHT44_000467"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ2532695.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGNRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13523..13807)
                     /locus_tag="WHT44_000468"
     CDS             complement(13523..13807)
                     /locus_tag="WHT44_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ2532696.1"
                     /translation="MKKLLLAAVVSLSAAAAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVYDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13958..15766)
                     /gene="aspS"
                     /locus_tag="WHT44_000469"
     CDS             complement(13958..15766)
                     /gene="aspS"
                     /locus_tag="WHT44_000469"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ2532697.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSSRNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAEFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVSNLSNGEDSGLQSPIVKYLSENALKEIIARTGAQ
                     NGDIIFFGADKAKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKATETKEV"
     gene            complement(15824..16525)
                     /locus_tag="WHT44_000470"
     CDS             complement(15824..16525)
                     /locus_tag="WHT44_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ2532698.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQPGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16668..17816)
                     /locus_tag="WHT44_000471"
     CDS             complement(16668..17816)
                     /locus_tag="WHT44_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ2532699.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGTGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(17977..18240)
                     /gene="rpsT"
                     /locus_tag="WHT44_000472"
     CDS             complement(17977..18240)
                     /gene="rpsT"
                     /locus_tag="WHT44_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ2532700.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18543..19685
                     /locus_tag="WHT44_000473"
     CDS             18543..19685
                     /locus_tag="WHT44_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ2532701.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LTPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5087 a         5104 c         4612 g         4989 t
ORIGIN      
        1 caatgccgta ctggtttttg ttaatccact ataaaatcga gtttatccta gctgtccagg
       61 acagccccta ttttttcata accatcacga aaggaatttt gcaaagattt tattccatct
      121 caaaacaatc atctcaaaaa tgcgtttctg accgccggta aaacaaaacc ctctaagaaa
      181 atacttagag ggttttgaaa tttggctccc cgacctgggc tcgaaccagg gacctgcgga
      241 ttaacagtcc gtcgctctac cgactgagct atcggggaac aagcgcgaat aataaaacag
      301 aaataccgaa cgtgtcaata taaattgaca atcgattgca ctttatctgc aagcccgcaa
      361 tctttatatg ttatatggct tccgcaatat gccttgccgc acgtttggca tccaatagga
      421 ttaaaccaag tctgccggtt tcttttgcca ccagcaccaa cacggcatct ttacccgcct
      481 gacttaaaag gatatagcct gattttcctt taatcatcac ttgttccaat tccccgcagg
      541 cgagttcctg caccgagcga ctccccaaag caagcaaagt ggcagaaatc gcccctaccc
      601 tgtccgaatt caaatgtgaa ggaagcattg tcgccatcgg caatccgtcg gttgagataa
      661 cggcagacgc gataatatcc gtagatgtat tgtttaaatc ttcaaggatt gaaatcaata
      721 attgctgcat aacccctcct ctctcccaag ttttacacgc ggttgctgta acggcggtat
      781 aaaatcctta ccaaagtaac aaatgcctct ttgcccaaat cgggaatgcc gccgataacc
      841 aaaataaatt tggttgaacc gatatacaat gggaaaaatg tcaattcgct ctgaccggaa
      901 ggatcgcaaa cgccccaagc gttattgttg atatacaggt tgttcttaat cagcagccgg
      961 tatttctttt ccatctgtgc gacttctgcc gccaacaacc ccaactcttc cgccgcctca
     1021 tgatggaaat tggcgttggc aagatacaga ccgttccgat cgaccaataa cgccttaccg
     1081 ctgccggaca attgttccat cagcaacggc aattgctcgt ccgacaaatt gatgccgtct
     1141 gaatgaccgt tttcatcgcc atagaggaat tcgagttttt gcaaacggta caacaggttc
     1201 aaagcggtat cgatgtcggc ggtgtccgcc caagtaagca gcttctcact gctgaccatt
     1261 tcgtccgcat ctgctttcaa caggctgtgc aacaaagttt taccggcact gggggcatcg
     1321 ctggatacgg cataaaatgc accggcagga gtcaggcggg gatataaatt tgcttgtagt
     1381 gaaagtgttg attccatatt aaacctccag tcccggatca atagaaaata acattgcgct
     1441 aaccaattgt tttacgtcat cttccttacg ggcatcaatt tcaaaaaccg gaacattaag
     1501 attatgtttt gcaagatatt tgtgatacac gtcgataccg ggctgagagc gtatatccat
     1561 cttggtaata ccgacaacga cgggtgcctt ctccagcagc cctcgaaacg aatgtaaaaa
     1621 gaattccaaa tctttcaacg gattggttcg ggcattatct aaaagcaaga ccaaacccat
     1681 actgccttgg cttaagattt cccacataaa gttgaaccgt tcctgaccgg gcgtaccata
     1741 taaatggact ttggtatcct catccaagct gatggccccg tagtccatcg ccactgtcgt
     1801 attccttttc ctatccaaag tcatatcgga tgcggaagca tcggtctgaa cgagtgcttc
     1861 gtccgaaata gccgcaatgg cagtggtttt ccctacgccg acaggtcctg tgaaaataat
     1921 tttattttct ctcatctctc cgcctcttag ctgcccaaca gttttttcat cagcctttgc
     1981 agaaggccgc gcgactggga ttgggatggt gttgtgattt tttccgcttt tttcatcatt
     2041 tcactatcag aggcagaatc ggctccgata tttattttat ccgccatatc ggagtatgcc
     2101 tgttgtgaaa ccgtttttaa atctaccgac aaaaacccgg ttgtataggt tgccgcaaga
     2161 taattcagaa tatcattgag gtttaaaggc atcactttat acaacacgtt aaggttgacg
     2221 gatgccttgg tcagaaatgc cgacaagcgt atcgaccccg gcacatttgc caaccgggtc
     2281 aggtttggcc aagatttcaa cgtaaacgga gtatcgggag aaatcggata aatcaacctg
     2341 ccctgcgctg tccaaatgga aaactgccac atacaggaca taatgcctac tttagccttt
     2401 tcgcgccatt gcgggttatc gggaacagtc ttgcagctga cctgcaaatt ttcgtctttg
     2461 cacaattctt cgagtttttg cacactttct gtcagcaaaa cccgttgtat cgaggggaaa
     2521 acaataagga ccggcttatt tccatgcaag atagcgatgt cctgcctgtt cttttccgca
     2581 aaccgcaacg cccccaataa tcctttattc gggttaaact ggcgtatcgt aaccgtacgc
     2641 tgcacattcc cgttattttt cgccgaccgg tctgcgggtg caataaacga tttcccataa
     2701 acattctcgc cctgcaacaa tttgcggagc ataggaaaca atgtttcaaa ccgaatcggt
     2761 ttgggcaggt agggaacttc agaatcggga actttctccg aacagacggc gacgggtata
     2821 tccttataac gctcggcaag ctctttccaa agttcaaaac cgccctcggc atcggtatcc
     2881 gccaaaacca aatcgggcac ggcactgccg tctgaagggg atactgtttc ataacgggtg
     2941 gtattgtgca ttttgaatgc cattttgaaa acggattcct gctgcgccgt catccccgcc
     3001 aacattacgc gtactgtttt aattttcggc agttgaactt ccatttttat tttccgtacc
     3061 gttttatttt tttaatattt tgattcatac gctgcagcag ccggctcatc agcataacga
     3121 cctcttcagg aagcctgtcc gcacgttccc tcaataccct taaaaactgc cccaacctat
     3181 cccaatcttc agtacgttca taaatatcga tcaacgtaat ataaagctgg gactcgtcgg
     3241 gatatttcaa taccgcctgc tccaacacat ccattgccgc ttcaatctga ccatacatca
     3301 gcaacgactc tacttcctta accgcatcgt ctgccggaga cgaaccggtg ttaatcaacg
     3361 aagaatcttg aagcaccaaa tcccgatgtt gcgatttgaa tttctgtata tttttcggca
     3421 gatacccgtg ccccataccg atgtctttga tttgccggtc gttcggccct ttttccaaat
     3481 catcgaaaac ttcatggtaa cccaagctgt acccccaccc cagcatccgc tctttaacct
     3541 gcctgccgta gttgcccaac gtttggtaaa gtttccacaa atgtccggca aaacggtcta
     3601 tgtccgcgtg ttggtaatcg agtttcaacg catcgataat caggtttgca ggtttttcgg
     3661 aagtttggat ggcacggtcg tattgtttcg atgccgtttc atagctgact ttgtctttaa
     3721 ggattttcgc accttgatcg gcacggacca aaccggcaat cgcaccgatt tcctcttgac
     3781 tgattccgga cacgtctttt ttgccccgca caatcgggat gcgcttgatt tcttcggctt
     3841 cataagcctt accgccggca tccggcgggg gcgatgcgga agctgccgtc gcagaaccgc
     3901 cttccgcacg tttttcaatc tcctgagtac cccatcccaa accttcttcc gccaagacgc
     3961 ggatacgcaa atggtttgaa tcgcgctgta atgcctgttc gatatatttt gccaaaagtt
     4021 cggaaagaat cagtttgccg tatttttgca gattgtctgc caaaacatcg acatccccca
     4081 cttcgagatt gatatcgagc agctcgcgga taaggttttc aggtttcgct tcaccatccg
     4141 gaatgccgtc cagataggca gccaaagatt cggcagcctt gccctgataa ccgaattgct
     4201 tataaacctg atactccgta agcggatcga cttcttgcgc ggatacggcg gcggatgcgg
     4261 tctcggcact ttcgttccaa gaccagtcgg gttgcgtgcc gtctgaaacc gattgtccaa
     4321 tctgatcgac ccagtccgaa tcattgcttc cgggtgtctg cccctgcgta ttgccgacac
     4381 cttgggattt gcgcttcggt gttttcttac cctgcttcgc acgcataacc aaaagcatca
     4441 gcaataccgc cagtgccaaa ccgataatta atgagttttc caaaggatac tcccgatacc
     4501 gtaacgagcg gatagctgac cgcctgcaac ggccattgtc cgcttttatt attaacgatt
     4561 tactgaaata gacttgtaag ttttaaatca taccataatt taacgtttaa caatatgcct
     4621 tctgcacaag cctcgccata ttacctttta cccacatcag tatcaatacc cgatataaaa
     4681 ataactttgc ccataagctg ccttattgcc tgcccgccgc agagtagcgc gcgataaaaa
     4741 ataacggatg ataagtcagg cgcactttgc ccgacggcat accgaacgcc gacaagtaat
     4801 cgccaataaa tccattgaga ttttttcttg tccaattttt ttggttcgtg gctgttacgc
     4861 ctgtatattt aaggtgtttg agtacatctg acggcgtatc aaagtctagt attaccgtaa
     4921 aatcctcaca ccataaaagc tcaaaatctt tggctaacca agcctgccat tgggataaag
     4981 tcgggtaatt taagcctata tttgtaattt ggcggacttc ttttaaattg tctttgccaa
     5041 aggttgcaac cgccaataat ccgtttgttt tcaagcctgt tttgcaatgg gtgataaaag
     5101 cgtcgggttg atgaaaccat tgcacggcag atgcgcttgc gattaagtca aattgtcgtt
     5161 gaaaaggaaa gttttccgca tcgccgcaat aaaaatcaaa ggattgcggc agtttttcag
     5221 ccagttgggg ctgcacattg cacaaatcat taaataacca ataattcgct gaaatctgtt
     5281 tttgcagcaa ggcactcaac attcctgagc cgcagcccaa ttccaacacg ttttccaatg
     5341 gcatatccgg caaataatct tgcaaatgcg tcattaaatt aatcgtcatt ttttgttgga
     5401 ttaaggcgtg ccggtcataa tcgtttaatg ctttttggaa agcctgccga atgcgcgatt
     5461 tatttatggc agtcagtgat tccatagtgc cgaccaatgg gtgaatcttg aaaacaggta
     5521 atgtccgacg tcaatttccc gaacggtgca acgcggtgtc caatatcggt gctgattggc
     5581 aggcataaaa attttatcgc ccgatccgac caaggcattt gtccagcgga taagatctgt
     5641 acgtctatct tgcccgatca tcgcaaaaag tgcgataagt tcttgatgaa tttcgccaaa
     5701 cgggcgtgcg ggaaattgtt ggtaatcttc aaaagatgct ttatcgccac acattctgcg
     5761 ttcaaatttt aaacgggtgt tttccgtgag gttctccaat gtgcctttaa aaacggtgca
     5821 agggataccg aaattatcat cgcaaggcaa acctgtgcca ttcactgccg ttgcggattt
     5881 taatcttatt ccttgcaatg ccctctctgc cgcccaaacg cccattgacc acgccaccaa
     5941 acggatgtgc cgataggcgg aaaaatcaaa atccaaattt aaatcttgat aatcatagca
     6001 aatcaataaa tcgtgatttt ccggcaaaat caaatgattt acagcatcgg gcggcgttcc
     6061 ccaacctgca aaatacagga ttaaatgtcc gccttgatga ttgtaaaatt ttgttttcat
     6121 atcatatcct tacaggcacg ccgcaaactg ccgcacttca tccgttgtca tatctgccgt
     6181 taaagacagg cggattctgg atgtgttttt gggtactgtc gacggtctga tgggcaggca
     6241 ataataaccc tgcctttgca ggtattccgc tttggcaagg gtggcttcat tcccgcctaa
     6301 aatatagggg acgatacagg tttggctcgg cattatttgc gtccgatgcg ccacttcccg
     6361 ccgtaaaaat gcgcttaact gctcaagatg gcttctttct tttgagaatt gcggcaatcg
     6421 ttcaaaaata aaataagtcc aagccacatt aaacggcggc aatgcggttg aaaaaatcaa
     6481 tgggcgcatt tgattaatca aacattcttt caatacttgg ttgcagacgg catacgcccc
     6541 caccgaggct aaggctttac cgaaagtgcc aaccaataaa tcaatctcgg caatcaaatt
     6601 atcccgttcg gcaatcccca atccgttttg cccataaaca ccgattgcgt gggcttcatc
     6661 cacataaaga taagtattgg gaaactgttt ttttaattgg acaagctgtt tcaaatccgc
     6721 cacatcgccg tccatactga aaacagattc ggtaacgata aaagtgcggt caaattttcc
     6781 gacgtttttt tcaagcagat ttttcaaatg ttcataatca ttatgacgat aacggaaaaa
     6841 cgcacaccgg ctcaaacgga tgccgtcaat catactggcg tgaacaaatt tatctgccaa
     6901 aatcaaactt ttcgtcgtcg tcaaagcagg caaaataccg agattggcgt gatagccgct
     6961 gttgaacaat aacgcgcttt cccgttggaa acgttgtgcg acaagctctt ccaaatcggt
     7021 ataaatagga aagttgcccg ttaataaacg cgatgaagaa ctggtaaaag agggaaaatt
     7081 accgccgtat tgctgcaaaa aagaccggcg caagttttca tctgatgcca aacccaaata
     7141 atcattagac gacatattca gcattttgcg gttttcccgc gtaatatacc gcccttgatg
     7201 aatcaaatcc ggaatcgaac gatattggtt ttgcgcgccg agttgttcaa gctgttgttt
     7261 aaaaaccttc attatgatgt aaatattcct gagttaaagc ctgaaccaac ccagcatcaa
     7321 acaatcaagt aatgcccttt taataaaaac cgttttgccg ccgacctttg ccacacccct
     7381 gccttcataa tccaaggcgg aaatttccgc gacatttgtt tccgttgcca ttacccaatc
     7441 cgtccgcact gtaccgaagc gcatattttc gcacaaattg gtacggtttc aggtatgatt
     7501 tgccgaaaac ctatcggaca cacaccatat tttgatatga ataaaacaat ctgccgcact
     7561 gctgcactat tgataagcgg tttttcatac gcaaacacca tcattcccga tgtctccccc
     7621 gtcgcacaag gtcaacatgt cttcattaac atccctcagc aacgcctgtt cctctacacc
     7681 gacggcaaac tgaccaaggt ttatcccgtt gcagtaggtc gggcgatgac gcaaaccaat
     7741 ctaggcgaac ataaaatcgg tgcgaaagcc tataaccctg tttggtacat ccccaaatcc
     7801 atacagaaag aacgcggcga cggcgtaaaa accatagccg ccggcccgga caaccctttg
     7861 gggccggttt ttgtccgctt aggcgacccc aaactcggtt tgggcataca cgggaccaat
     7921 gcgccggcca gcgtccccgg ggttcggagt cacggctgcg tccgcatgaa atcgcccgac
     7981 gcgctcgagt ttgccaaaac catcgccagc ggctcgcccg cctccgtcat ctatcaaatg
     8041 gcgggtctca atgaagatgc ggatcgcaac ctgtggcttg ccgccttccg cgacccttac
     8101 ggtaagaaca accttgacat cgcctctctg aaaaaaagta ttgcgcaatg ggcaaaaaca
     8161 cagggtaaaa ccatcgcgcc cgagaaagtc gatgctgtac tcaaagaccg caccggatcg
     8221 gccgtctgcc tgacctgcgg caaaaacggc aagatgaaga tgccgctcaa atcgctggcg
     8281 tggatacagg gttcttcctc atacagccaa gctgaagtca ttgaacaaac tgaggaaacc
     8341 aactctgccg aaatatccga gacacgcacg cccgaagtgc ctgatgtaca cacgcccgag
     8401 gcacaaccgc atttaaatac ccaatccgac ggcacgccga ctgcctatac cgaaccggct
     8461 gccgattcat cgccgcaagt agaaacacct gatcaggctg cttccgagcc ggttgatgta
     8521 ttattttcaa tagatgtgat acggcaggga aatttgcgtt taggtaattg aataaccttc
     8581 tgattattta atgcttatgt ttatcagaag ttgataggcg gtttggtttt tgtttggctt
     8641 ttcctacacc gccgcctgcc gctttctgca acattcaagc gcacaaatat gccatctgaa
     8701 ggctttagac ggcatatttc acgataatag tcgcccaatc aaataaacac agattggaaa
     8761 tttattttat ttttccctta ccaatacccc agaactttcc accaaatgct gccgataacg
     8821 aaaaaaatca gaaaattgac tacgctcatg ataaaacccg ccttccacca ttctcccatt
     8881 gtggtgtagc ccgaaccgaa aatcacaggc gaagtaccgg tcgcataatg agtgagggtc
     8941 atcataatgt tggatgcggc cgccatcatc agcgcggtcg gcatcgccgg ggcattcagt
     9001 gaaacggcag cagcgaaaaa tgcgccgaac atagcggtaa tatgtgcagt agtactggca
     9061 aacatataat gcgcatacat ataagcaagc acgaggatta cgcccgcagc cgtgccgcta
     9121 acgcccaaac cgccgacact ttccgccaac actccggaga accatttaat cagtccgagt
     9181 ttatttaaaa atgcggccat cataatcaat gcgccaaacc aaataatcgt atcccacgcg
     9241 cttttttctt tcaaaacatc gtcccaagtc aatacaccgg aaagcaaaag caggcttaat
     9301 ccgataaatg cggtggcggt ggcgttgata ctaaaagcgt gattgccggt aataagggcg
     9361 ggaacatctg cccacaacag cagcaagata ccgaaaatga ccgccataat gatttcgtct
     9421 gccgacattt tacccatctc cctcagacgg tctttggcaa attgaacggc attgggcgtt
     9481 tctttaattt caggcggata caaaaaatat aaaatcaaag gcataacgaa aaaggcgata
     9541 acgccgggaa cagccattgc ccacgcccac gccccccaag aaagacggaa actactgcct
     9601 aaattttcgg caatcaagtt gacgattaaa gggttggggg cagttgcagt aataaacata
     9661 gccgacgaaa tgggattgga atgatagttg accaaagcca aatatttacc catcttgcct
     9721 tctgtgcctt ttgcgggatt ggagccgtaa ctgccggcaa tcgactgcat aatcggatgt
     9781 ataatgccgc cgccgcgcgc ggtattggaa ggggtaacgg gagccagcag cagttcggaa
     9841 agagcgagac tgtaaccgat gcccagcgtt tttcttccaa aaacggcgat aaacaaatat
     9901 ccgatacgca tccccagccc tgttttgagc aaaccgcgcg aaatcataac tgcgatggca
     9961 atcagccaaa tcaacggatt ggcgaacgca ctcaacgcat cgctcatcgc cgcgcccggt
    10021 ttgtcggcgg ttacgccggt tactgcgacc aacccgacgg caataatcga cagcgcgccc
    10081 aacggcatgg ccttgccgat aatggcggca atcacaccga caaacatggc cagcagcgtc
    10141 caagcctgag gcttgacccc gtcgggtacg ggcagtgcca aaaccagggc gcacaatact
    10201 gcggcaatgg cgaggggtat cggtttgaaa cccaatttca tcatattgac ctccgtaaaa
    10261 aagaccgtcc cgaaaaatcg gaaaaataat atttaactaa ttgttttata agatatattc
    10321 tgatatttca ccgtctttcc gatatgcggc tccgggcaac ttttgtttca gtatttgaat
    10381 tttcattaga ctgaatacgc cgtttgaacg gcacggcgaa aacccggggg atgccggacg
    10441 ttcagtcctt tttcgcacct tgaaggtaaa gcatttccaa ggcaattgtg gcaccggcca
    10501 aagcggtaat gtcggattgg tcgtaagagg gggcaacttc tacaacatcc ataccgacga
    10561 tgtcgagatc cgtcagccca cgtaggattt ttaatgccct gtcgctgctc aagccgccgc
    10621 atacgggcgt accggtccca ggggcgaacg acgggtccag gcagtctatg tcgaaagtca
    10681 ggtaaacggg catattgccg acggtttctt tgattttacg gacggtctct tcaacactgt
    10741 cttcattgac tttaggggcg gacaacacag taaaaggcaa ttttttactg tgttcggtgc
    10801 gtatgccgat ttgtacggaa cgggacgggt cgatgaggcc ttccttgggg gcggtataga
    10861 acatcgtacc gtggtcgtat tcgctgccgt tgtcgtaggt gtcggtgtgc gcgtcaaaat
    10921 gaatcagtgc gagtttgccg aaatagcggg cgtgggcgcg caacaacggt agggtaatga
    10981 aatggtcgcc gcccaaactc aaacagcgtt tgccggaaga aagtaatttg ccggcgtgcg
    11041 cttccatttt ttcgacaaaa tccctgctgt cgccaaaaga aaaaaccaag tcgccgcaat
    11101 caataatgtt caggcgttcg cgcacatcaa atgtccatgg aaacctgcgg tgctcccaag
    11161 cgaggttgac ggaggcgcgc cggatggctt caggaccgaa acgcgcgccg gaacgccctg
    11221 aaaccgccat atcataaggc acgccggtaa taacccaatc ggcatgactt tcatacggca
    11281 taaaattaag cggcaggcgc aaaaacccga aattattgga aacgagggag ttgtcggttt
    11341 gtcctgccag tgtgctgtat tgcatcgtaa tgattccttg taattggttt caatcggtcg
    11401 tgatgattgg tgtttgagta agaaaatcgg gcttcagacg acatatccga tgccttgatg
    11461 cgtctattcg tcttccaaat aggtataacc attaagcccc gcttcgagtt cttttaagaa
    11521 agacatagcc tgcgaggcag gaaggtctga atgttcgatt tgttcgcgat agcgtttcat
    11581 cagctctttc ggatcttgat aaacgtattc gagcatatcg gcaacggtgt ttccttcatc
    11641 gtaatcgatg acggtaaatt gtccgtcttc ccctacaaca acatcggcag tggcagtgtc
    11701 gccgaaaaga ttgtgcatat tgccgagtat ttcctgatat gctcccacca taaaaaagcc
    11761 taaaagcggc ggctcttctt cgggataatc aggcataggc atcgtaccgg cgatgccgtc
    11821 tccgtcgatg tagtggtcaa tcgtaccgtc tgaatcgcag gtaatgtcca acaacacggc
    11881 gcggcgcgca atcggttcat tcaaaccggt aatgggacaa acagggaaaa gttgatctat
    11941 gccccaagca tcgggcaaag attggaagag tgagaaattg acatacagct tatcggcaaa
    12001 acgttcttgc aattcgtcaa taatggttcg gtgagaccgg tgtttttcat taaacaattc
    12061 gccgacttca tgacagatat ttaaatacag ttgctccgcc cacgcacgtt gcgccaaact
    12121 caacagcccg acattatact gattatgcac atcagcaaga tcaaactgcc cttcgtgtat
    12181 ccagctgcgt aaggaacgtt tttcccgcga ggcggaaata tccgtccaag tttcccacat
    12241 actgtgcaac acacgcggtg cttcgggcga tggcgcatcc agccggcgcg gtttgtaacg
    12301 ttcaacgcct ataacattag caaccaaaac ggcgtgatgt gcggtaatgc cgcgcccgct
    12361 ctcggtgatg attgtcggat gcggcagccc gtgttcgaga caagcctgac tgatgcccca
    12421 tacgactgtg gcggcatatt cgttgaggct gtaattaacg gaacaatccg attgtgtgcg
    12481 gtttccttcg taatccacgc caagcccgcc gcctacatca aaacagcgga tatttacccc
    12541 cagtttgtgc aactcaacat aaaaccgagc cgattcgtgt acacctgtgg caacatcacg
    12601 gatgttccca agctgcgagc ccaaatggaa atgcaaaagc tgcaggcaat ccagcctgtt
    12661 tttttgtttc aaaatatcga ccagttgcaa aacttgggaa gccgacaagc cgaattttga
    12721 tttttcccca cccgaagact gccattttcc cgaaccttgg gaagccagtc tggcgcgcac
    12781 acccaaacgg ggcttgatgc cgagtttttc cgcctcttcc aataccattt gtatttcgga
    12841 cagcttctca atcaccaaat aaacctgatg ccccagtttt tcgcccatca aggcgaaacg
    12901 gatatattca cggtctttat agccgttgca gacgattaat gtttgccggt tgccggcgtg
    12961 tgccaaaacc gccatcagtt cggctttaga accagcttcc aaaccatgcg gttgtccgct
    13021 tgacataagc gattcgatga cgcggcggtg ttggttgacc ttgatagggt aaaccaaaca
    13081 ataaccgccc ttatagccgc actcttcccg tgccgtctga aaggcgcggt taatgtcgcg
    13141 gaggcggtgt tcgaggattt gcggaaaaca aaacaaaaca ggcaggcgcg cctgatgttt
    13201 ttgttgcacg gcttcagtca gtttttgcag tgaaacagtt tgattgtgtt gcgagggatt
    13261 ggggcggacg atgatttcgc cggaatcgtc aacatcataa taacctatgc cccaatgatt
    13321 aatgttgcac acttcacgga tggtaaggat aggcataata aacctgctcc gtctgtcgtg
    13381 ttgaaaagga atgattataa caaatcagcg tgaaatgtca tttttttaat aagaaaagcc
    13441 tgcctcatac ctgatgagga acaggcaaaa tgccgtctga acgcttcaga cggcattttg
    13501 gttcatcttt ccatcaaagg agtcagcgat cgagctgctc tttgatgatt ttcaggtcgg
    13561 ggtaagacaa cacgatgtcg tattcgcggc cgtctttata ggcttccact tccagcacag
    13621 gcttacccca atggtcgtcg gcatcgacat cgtaaacctg ataaccgcgc tgctccaaca
    13681 ttttcacagc ttttgtgcgg ttttgttcaa aatggggatc gccgtaaatc tgacgctcgg
    13741 cagagtcgcc ggcaaatgcg gcagcggcac tcagagaaac aacggcagcc aataacagtt
    13801 ttttcatttt cagtcctttt tttatcggtt gattgaacaa gatgtgtttt tcaataccgc
    13861 cattaaaaca cagcaaaatt aggtttgaat tagagggaag tcaagaagcg taaatgccgt
    13921 ctgaaaaaaa cagagccgtc aaacggctcc gttttcctta tacttcttta gtttcggttg
    13981 ccttctggcg caaacgcaaa cttaattcac gcaactgctt gtcgtccacg ctgttgggcg
    14041 cgttggtcag caggcattgg gcgcgttgtg ttttcgggaa ggcaatcacg tcgcggatgg
    14101 attcggcacc ggtcatcagc gttaccagac ggtcgaggcc gaatgcaaga ccgccgtgag
    14161 gaggtgcgcc gaatttcagg ttgtccaaga ggaagccgaa tttctcttgt tgctcttcag
    14221 ggctgatttt cagcgcggca aacactttct cttgtacgtc tgcgcggtga atacggatag
    14281 agccgccgcc gatttcccag ccgttcaata ccatatcgta ggcgcgtgcc aaacaatttg
    14341 ccgggtcgga aaccatcagg tcttcatgac cttcttttgg cgcggtaaac ggatggtgta
    14401 cggcaacgta gcggtcggct tcttcgtcgt attcgaacat tgggaaatca acgacccaca
    14461 aaggtttcca ttcgtctgtg aaatagccgt tgtctttgcc gtgctccaag ccgactttga
    14521 tacgcagtgc gccgatggct tcgttcacga ctttggcttt gtctgcgccg aagaagatga
    14581 tgtcgccgtt ttgcgcgccg gtacgcgcga taatttcttt cagggcgttt tcggacaggt
    14641 atttcacgat tggagattgc aggccgctgt cttcgccgtt ggaaaggttg ctgacatcgt
    14701 ttactttgat gtatgccaga cctttcgcgc cgtagatgcc gacaaatttg gtgtattcgt
    14761 cgatttcttt gcggctgaat tctgcgccgt tcggcacgcg cagagcgacc acgcggccgc
    14821 ctttcatgtc ggctgcgcca cggaagactt tgaattcttc cgttttcatc aggtcggtca
    14881 actcggtaaa tttcaagttg atgcgcatat ccggtttgtc agagccgtag tagaacatgg
    14941 cttcagagta aggcatgcgt gggaagtcgc ccaaatctac atttaaagca tctttgaaga
    15001 cttgtttggc catgccttca gtgatgtcca tgatttcatc ctcgtttaag aacgaggttt
    15061 ccaagtcgat ttgggtaaat tcgggctggc ggtcggcacg caggtcttcg tcgcggaagc
    15121 acttggtgat ttggtagtaa cggtcgaaac ccgccaccat caacagttgt ttgaataatt
    15181 gcggcgattg cggtagcgcg aaaaactcgc ccggatgaac gcggctcggc acgaggtagt
    15241 cgcgcgcgcc ttcaggcgtg gagcgggtca gcatcggggt ttcaatgtcg atgaaacctt
    15301 gcgcgtccaa gtagcggcga acgcccatag caacttggta acgcaggcgc aggttgcgtt
    15361 gcatcaccgg acggcgcaag tcgataacgc ggttggtcag gcgaacgttt tcgctgatgt
    15421 tttcatcgtc gatttggaac ggcggcgtgg cggcggcgtt caagacttcg atttctttgg
    15481 caaggatttc gattttgccg gaaatcattt tatcgttggt cgtgccttcg ggacggttgc
    15541 gtacgcggcc ggtaatgctc aaaacgtatt cgttgcggga ggaatcggca gcggcaaacg
    15601 cttcgggcgt gtcgggatcg atcacgactt ggacgatgcc ttcgcggtcg cgcaggtcga
    15661 taaaaatcac accgccgtgg tcgcgtcgac ggtgtaccca gcctttgacg gtaacggttt
    15721 ggtctaagta ttgctcactg atcaggccgc aatagttggt acgcataaaa tcacctttta
    15781 ttgatttaaa ctgaaaacag aaaatgccgt ctgaacggcg gctttattgt tgttcgggca
    15841 aatccgcctt ttcagacggc ataggtcctg ccaatgtttt gacgggcagg tcgtcaggga
    15901 tgaccatacc cagcgaaatc acatatttca atgcttcgtc cacgctcata tcgagttcgc
    15961 gcacatcgct tttctttacc ataatatagt aaccgccggt cggattcggc gtggtcggaa
    16021 catacacgga aagataatcg ccgtccttcg gcaatgcggc cttaaccgca ttcgacacct
    16081 gccctgacac gaaagcaatc gtccaaatac cgggctgggg aaacggcacg agtaccggcg
    16141 ttttaaacga acggctgctg tcggacagca gcgattcgga tacttttttc acactcgaat
    16201 agatggattt cacaaccgga atccgcccca acaggctgtc ccacgcggcg aggatctgcc
    16261 gacccaatac gttggcggca aacaatccgg ttacaaacaa tacggcaatg gcaacgataa
    16321 cgcccagccc cgggatatta aaccccaaaa catattgcgg ccgccattgc ttcggcagca
    16381 ggttgacgag ctgatcggac gcggaaacga tataggaaac cacccaaacc gttaccgcaa
    16441 tcggcagcca gaccaaaatg cccgtaatca gatatttttt taacgccttg gcagctttgc
    16501 cgccttcggc cgcaggttcc gtcatcttgc ttgattccga caaagtccgt acaaaccgca
    16561 cattatacgc gtttgcccgg attcaaacga aatttttatc ccgccccgcc aaaccgccgg
    16621 cggcttcaga cggcacggca acttgatata ccgtctgaac acgcggttca gatgccgtcc
    16681 aagtcgttga acatcaaccc gataccgata ccgttctgct tgtggttgta gtcgatcagg
    16741 ctctcgccgt aaccgtggaa tccgcgtacc acgcctttga gtttgccctt aatcggaaac
    16801 gtgtaggcgg cttcaatcgc gccgtagccc gttttggggt tgtagcgcaa tacggaatac
    16861 acattctgcc tgtcgttcag gcggtactgc agcttcacgt cgccataccc catatagtcg
    16921 gcaatatcgg gattgtcgtt tttatcgccg ctctgatcga acgcacgcac ccacacgcgc
    16981 ggaatcaccg tcaatttgcc ccattccatg cctgccatgg cgtaaatcct gttccacgaa
    17041 cgcgattcgg gacggctctg tccgttggac tggtggacaa aacccgtacc gagcatacgc
    17101 agcctgccgc cgaacggcaa atccgccttc acaggctggg tcaggaaaat ttcaggtttg
    17161 taatccgtat tgcggaacgg cgcggatttc ctgccttggt tgtaaatctg ccaatcggat
    17221 ctttgggtgt agccgaacca cagatccgcg cgggttttaa acaaatcttc ggcaattttg
    17281 cttttgaacg aaacctgcaa tttggtttcc gcacgtttct gctgtccgaa tttttcctgt
    17341 acagtcgtac cgcgcgtcgg cgaacccggg gcatagttgg gcgaattgtt gtaccagagc
    17401 ggcataaggt acatcggatt gtgttcgcgt acgcccaaca gcccgcgcaa atcgtttttg
    17461 tccaagtcgt acatcaggct caaaggcgta tagatgtcgg cggtttcgcc cgcactgtcg
    17521 gcaggaagcg catccccgcc tttttcaaca acaatgaccg cctcgccctt atccaggctg
    17581 ctgcggacgg tttccgtcag attgagtacg gctttcgact cctgcccttc ctgccctgcc
    17641 gaagacggaa gctgtgccgc aaaaatcctg tcgtaacacg ccaaacgcgt aacattgtcc
    17701 gtcaaagcgg cgcattgcag cgcggtctct ccaaaagcgg atgccatcgg caacagtcct
    17761 gtcaaaagaa tatagcgcat attccgtgta ttcatctccg cccccattgt cggcatattg
    17821 gttttcaaac ggcattttat agcggattcg gataaaaaat cgcacccttt ccgccatttc
    17881 gggattttgc ccccgcaata cagaaaaccc cgaaaccgtc gggcttcagg gttttccgct
    17941 tatcgcgtat caaccgcctt ggcggttttg caaaaatcaa gccaaggctt ttacttttgc
    18001 agacagacgg cttttgtggc gtgccgcttt gtttttgtgg aacacgccct tgtcggcgat
    18061 gcggtcgatg actttgacgg actcttggta aaccgcttgt gcggcagctt tatcgcctgc
    18121 ttcgactgct ttcaatactt ttttcactgc ggtgcggaat gcggtacgca ggctagcatt
    18181 gtgggcgcgt tgtttgacgg actggcgggc acgtttgcgt gcttgtgcgc tgtttgccat
    18241 attggaatct cctagaagaa aaataaattc tgtaaacgcg caattttaaa gggggtttac
    18301 attatatgca agccttttct ctctatccgc ccgtcggaca ccctgacagc gcaaaaaaac
    18361 ggcaaaatcg ggcggacgtt tttctcccct tttggaaaca ggataatttt ttaggaaaat
    18421 cagtccaata tcgctttgag tttgcacaaa tttactttac aatgcccaaa ccttgcgccg
    18481 gggtaacgat ggggcgcggg gatttctttt tttccaacct ctttcatcag gaatctgccc
    18541 gtatgaaaaa atccgtatta gccgttttgg ccgcgctgtc tctggcagcc tgcggcggca
    18601 gcgaaaaaaa cgccgtgcaa cctcaagcag acgctgcgtc tgctgccaat gccgaagccg
    18661 ctgccaccga tactctgaac atctacaact ggtcgaacta tgttgatgag agtacggtcg
    18721 aagactttaa aaaagccaac aatctgaagc tgacttacga cttgtacgaa aacaatgaaa
    18781 cgctggaagc caaaatgctg accggcaaat ccggttacga tttggtcgtg ccgggcatcg
    18841 ccttcctgcc gcgccaaatc gaggcgggcg cgtatcaaaa agtcaacaaa gacctgattc
    18901 ccaactataa aaacatcgat cccgaactct tgaaaatgct ggaaaccgcc gacccgggca
    18961 accagtatgc cgtcccctat ttctccggcg tgaacacgat tgcgattacg gcgaagggca
    19021 aagagctttt gggcggcaag ctgcccgaaa acggctggga tttgctgttc aaacccgaat
    19081 acacccacaa gctgaaatcc tgcggcatcg ccctgtggga caccccgagt gaaatgttcc
    19141 cgattttgct gaactacttg ggcaaagacc ccaaaggctc gaatcctgaa gacttgaagg
    19201 cggcggcgga agtgttgaag tctatccgtc cggatgtcaa acgtttcagc ccgtccatca
    19261 tcgacgagct ggcacgcggc gacatctgcc tggcggcagg caacggcggc gatttgaact
    19321 tggcgaaagc acgttccgag gaagtgaaaa acaacgtcgg catcgaagtg ctgacaccga
    19381 aaggtatggg cttctggatt gagtcttggc tgattcccgc cgatgcgaaa aacgtcgcca
    19441 atgcccacaa atacatcaac tacacgctcg accccgaaat cgcggcgaaa aacggcatcg
    19501 ccgtaacctt tgcccccgcc agcaaaccgg cgcgcgaaaa aatgcctgcc gagctggtga
    19561 acacccgttc catcttcccg aacgagcagg atatgaaaga cggtttcgtg atgccgcaaa
    19621 tgagcacgga tgcgaaaaaa ctgtctgtca gcctgtggca gaaaatcaaa gtcggcacca
    19681 actgatttga agcattaaaa atgccgtccg aacgatgttc ggacggcatt ttatattgga
    19741 ttgaaataga aatatttata tagtggatta acaaaaatca ggacaaggcg ac
//
LOCUS       DAWGRZ010000019        19236 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000019 DAWGRZ010000000
VERSION     DAWGRZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19236
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.019"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(173..1948)
                     /gene="ptsP"
                     /locus_tag="WHT44_000474"
     CDS             complement(173..1948)
                     /gene="ptsP"
                     /locus_tag="WHT44_000474"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ2532702.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIVKVIRCEDE
                     EKSEKLIKQMNSVSVEEEADFKGRK"
     gene            complement(1948..2217)
                     /locus_tag="WHT44_000475"
     CDS             complement(1948..2217)
                     /locus_tag="WHT44_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ2532703.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(2286..2723)
                     /locus_tag="WHT44_000476"
     CDS             complement(2286..2723)
                     /locus_tag="WHT44_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ2532704.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DINNNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(2805..3368)
                     /locus_tag="WHT44_000477"
     CDS             complement(2805..3368)
                     /locus_tag="WHT44_000477"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ2532705.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALGGK"
     gene            complement(3437..4261)
                     /locus_tag="WHT44_000478"
     CDS             complement(3437..4261)
                     /locus_tag="WHT44_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ2532706.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLMSRQGYAFAPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            4353..4985
                     /locus_tag="WHT44_000479"
     CDS             4353..4985
                     /locus_tag="WHT44_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ2532707.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFTLPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(5190..6974)
                     /gene="rmuC"
                     /locus_tag="WHT44_000480"
     CDS             complement(5190..6974)
                     /gene="rmuC"
                     /locus_tag="WHT44_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ2532708.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFADLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLADVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQHDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQSSFQTAFKQLAEGRGNLVGRAEKLRLLGVKANKQ
                     LARDLTERANETTALSESLEYAAEDEAV"
     gene            complement(7313..8101)
                     /locus_tag="WHT44_000481"
     CDS             complement(7313..8101)
                     /locus_tag="WHT44_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ2532709.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            complement(8116..9465)
                     /locus_tag="WHT44_000482"
     CDS             complement(8116..9465)
                     /locus_tag="WHT44_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ2532710.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            complement(9484..10065)
                     /gene="petA"
                     /locus_tag="WHT44_000483"
     CDS             complement(9484..10065)
                     /gene="petA"
                     /locus_tag="WHT44_000483"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ2532711.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(10188..10937)
                     /locus_tag="WHT44_000484"
     CDS             complement(10188..10937)
                     /locus_tag="WHT44_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ2532712.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            11068..11997
                     /locus_tag="WHT44_000485"
     CDS             11068..11997
                     /locus_tag="WHT44_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ2532713.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGAPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQSGIS
                     FQPLFAYEMVGICAKDHPLAAKTVWEAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            complement(12252..12644)
                     /gene="rpsI"
                     /locus_tag="WHT44_000486"
     CDS             complement(12252..12644)
                     /gene="rpsI"
                     /locus_tag="WHT44_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ2532714.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(12654..13085)
                     /gene="rplM"
                     /locus_tag="WHT44_000487"
     CDS             complement(12654..13085)
                     /gene="rplM"
                     /locus_tag="WHT44_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ2532715.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(13263..13442)
                     /gene="ssrS"
                     /locus_tag="WHT44_000488"
     ncRNA           complement(13263..13442)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WHT44_000488"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(13448..13753)
                     /locus_tag="WHT44_000489"
     CDS             complement(13448..13753)
                     /locus_tag="WHT44_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ2532716.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(13764..14093)
                     /locus_tag="WHT44_000490"
     CDS             complement(13764..14093)
                     /locus_tag="WHT44_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532717.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(14148..15137)
                     /locus_tag="WHT44_000491"
     CDS             complement(14148..15137)
                     /locus_tag="WHT44_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ2532718.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(15260..17962)
                     /gene="ppc"
                     /locus_tag="WHT44_000492"
     CDS             complement(15260..17962)
                     /gene="ppc"
                     /locus_tag="WHT44_000492"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ2532719.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPGVRVPDILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNGDVTALSDKSPDEEIARAEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNRLNEEQKQTALLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKETGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPDKAKIIFGMIKEEYRRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            18132..18902
                     /locus_tag="WHT44_000493"
     CDS             18132..18902
                     /locus_tag="WHT44_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ2532720.1"
                     /translation="MTTTEHDNDDAFLLRYSRHILLDEIGIEGQQKLSAAHILVVGCG
                     GLGAAALPYLAASGVGTLTIADSDTVELHNLQRQVAFDEGDVGKLKTEALAGRLKRIN
                     HTVNVRAVNEKLDGCRLTGLVQAADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVR
                     FEGQLAVYRPDLPDSPCYACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILL
                     DAGEPSHGRLAVYRALEGGWQYFDLPRNPECPVCGTAR"
BASE COUNT         4105 a         5253 c         5110 g         4768 t
ORIGIN      
        1 acctagattc ccgcctgcgc gggaatgacg gattttaggt ttctgatttt ggttttctgt
       61 ttttgaggga atggccgatt ttgggtttct gtttcggttt tctattttgc aagaatggca
      121 aaatttcaga ttgcgggcat tgttaagtat ttctattttt tacctgccgt atttatttcc
      181 gccccttgaa gtcggcttct tcctcgacag acacgctgtt catctgtttg atcagctttt
      241 ccgacttctc ttcgtcttcg cagcggatga ctttcacaat atcgctttcg agctgtccga
      301 cattgctgtg cagaatgatg tttttgacgg gcaggatgtt gttggggttc atggaaaaac
      361 ggcgcagccc catacccaat aaaacgcggg taaacgcggt atcgcccgcc atctcgccgc
      421 atacggatac gtctttgtcc atgcggttgg cggtacggat gacgtgttgc agcattttca
      481 gcacggcggg atggccgggc tggtagaggt ggctgacgct gtcgtcgccg cgatcgacgg
      541 acaagatgta ttgaatcagg tcgttggtac cgacggagat gaaatcgacc agtttcaaaa
      601 tactgccgac ggtcagcgcg gcagacggaa tttcaatcat acagccgatg ccgactttac
      661 cgaaggcatc gccgcgttcg gcaagctggc gttgcgcggt gtcgaggtgg atgaggcact
      721 ggcgcacttc ggatacggag gtaatcatcg gccacatcat ccgcacgggg ccgtgtaccg
      781 ccgcacggag gatggcgcgc atctgggtgc ggaacatgac cggttcggca aggcacaggc
      841 ggatgccggt catgcccagc gcggggttga ggctgccgtt gggcgtgctg tttttcccga
      901 accagcgcgg gtttttgtcc acacctaaat cgactgtccg tatcgttacg cttttgcctt
      961 tcattttttt gacaatcgcg ctgtacactt cgtactgctc gtcttcagac ggcatcgtat
     1021 cgcggttcag gtaaagaaac tcgctgcgga acagcccgat gccgtctgcg ccgaggttgt
     1081 gcagcggttt cacgtcttcg gcggattcta tattgcccac aagctcgatg cagaccccgt
     1141 cggcggtggc ggcggcggtt tttttgagct tgttcaaatc gcgtttgtgg ctgcggtatt
     1201 cgcgggcacg gcggcggtat tcgttcaaca ccgactcatc cggcgcgata atcaacacgc
     1261 cgttgatacc gtccacaatg accgtttcgc cttcggtaat cagtttgcgc gcgttgtgca
     1321 gcccgacgac ggacgggatg tccaagctcc tgcccaaaat cgccgtatgc ccggtggggc
     1381 cgccggcatc ggtaacgaag gcggcaatgc gctgctcttt aaacaaaacc gtgtcggcgg
     1441 gcgaaaggtc gtttgcaatc agaacggttt cgtcaaacag gttgtcggca acttccaact
     1501 cgttgccctg cccgatcagg ttgttgtgga tgcggcggac gacttgcagc atatcctgct
     1561 tgcgttcgcg caaataggca tcgtccatat tgtcgaattg ggcggcgagt ttgtcgctct
     1621 gctgcttcaa tgcccactcg gcgttgattt tttgttccct taaaatatcg acgggttcgc
     1681 gcgacaaggt aacatcggtc aagagcatca ggtgtagcga gatgaacgcg cccaactcgg
     1741 tcggggcgtt ttcgggaatc gcgctgcgga gctgttccaa ctctttgcgc gtggctttga
     1801 cggcggcatc gaaacgttcg gcttcggcat cggtgtccgc ctccgcaaca tcatactgcg
     1861 gcacttcctc cgtaccgcgc gcaatcaggt gggcgcaacc gacggcaatg cctttgcccg
     1921 ccgccacgcc gtgcagcacg atactcatta ttcgccctcg ccgaagtagc cgttgattaa
     1981 gtcggtcagg gcgcgcatcg cttccgcctc gtccgcgccg tccgtctcca gttcgatgac
     2041 cgtacccttg gcggcggcga gcatcatcag ccccataatg cttttgccgt tgacgcggct
     2101 gtcgtttttc gtaacccaga cttcgctttt gaattgggac gcggtttggg tgaacttgtt
     2161 ggacgcgcgg gcgtggagtc cgagtttgtt gatgatttcg atggattgtt tgagcatttc
     2221 gattcccgtg ttatgtatat cggcagcaga cgccgtttaa aatgttttcc tgccctgccg
     2281 cttcttcaga cggcatcgcc gctgcgccgg cacaccaaat cttcgggcgc ggacgtgatg
     2341 gcgaaaatgc cttttaccgc cgcctccctg acgcattcgg taaaggcggc aaggtcttcc
     2401 gccgccggcg aatattggac ggccttaacc atcatcggcg cgttcagccc ggtcaaaatc
     2461 gccgatttgt tttcgcgcac gaggcggcgg gcggcattgc agggggtcgc accgaaaata
     2521 tcggtcataa tcagcacgcc gtcgttgtcg ggaaattcct gaagcgcggc aatggcgttg
     2581 ttgttgatgt cgtcttggtc ttccgtcggc tgcacgccga gtatgcggac gttttcaggc
     2641 agtccgcccg gaaaaaaatg atgcgccagc ttgcggtagg cttcgcctat ggtttcgtgt
     2701 gtgatgatta aaagccctat catattatgc gtcctgttcc tcattatcct gccggcgtat
     2761 gggcgcgatg ccgtctgaac agccttcaga cggcatcgcg ccccttattt tccgcccaat
     2821 gcgtaaatct cgcccagatt gcgccagcag cccgccgcat ccatgccgta accgaaaaca
     2881 taacggttcg gcacatccag tccgacataa tcggctcgga taggcttggg tttgtcaatc
     2941 agcttgttgg cgaacaccgc cgcacggcag cttgccgcac ccatttccaa aagtttggct
     3001 tgaatggcgg acatcgtatg cccttcgtcc aaaatatcgt ccagcacgac gacgtgcctg
     3061 ccccggattt gttccgcatc gggcatacgc ttccagttga acgcgccgcc ctccagcttg
     3121 tcgccgtaac gggaaacgtg aacataatca aaatctaagg gaaaacgcaa cagcggcagc
     3181 aactgccccg taaacaccac cgcgccgccc atcacgggca gcagcagcgg atatttgccg
     3241 cccaaatcac gcgtaatctc gtccgccact ttttgcagtg cggcacggca ttggccttgg
     3301 tcgaacaaaa gatcggcgtt ttcaagcatc gcctgtgttt caaggcgttt ggtttctaaa
     3361 tcggtcatat gtcggaatcg gtcggtaaaa ggaaaattat aaaccaaagt atcggatgcc
     3421 gtctgaactg tcctgctcag cggtcggtac gcacgcgcac aaatgtggca aatttcggcg
     3481 tgcctttccg cgtaaagcca cggtaacggt aggtaatcag tgtgccgatt ttgggcgggt
     3541 tgtcgcggtc tttatctttg aaaccgctgc cgatgcggaa ttcgccgtgc cggtttttgc
     3601 agccgaccgc gcccagccgt ccggcgtttc gccctttgcc ctcatagtgc cgcgttaccg
     3661 tgcattcgtc gtcgtattgg cttttcagct tcaataattg gctgctcctg ccgccgctgt
     3721 aacgggattc gggctgacgc agcatcacgc cttcgccgcc ctgcgcttcg atttgtttta
     3781 aaaagtccat cgcgtgctgc cggtcgcgca ctttgatttg cgggatgatg gtaatcggcg
     3841 cgttcggatg cgttttcagc cactgcgttg cgactgccaa acgttggtag aggttgccct
     3901 gcgccttggg tacatcgaaa acgtgcaggc ggatgccgcg ccagtctgaa gaaacagaac
     3961 gcacggtagc ggaaatctgc tcgaactgac cacgtccgct atacaattcg ccgtccaaag
     4021 gataaggcgg aaactgagcg gtaaaacctt tgggcggagc aaacgcgtag ccctgacggc
     4081 tcatcaggtg ctttccgtcc caataggcgc gcacgccgtc gagtttctcg ctcatcgccc
     4141 agccggcaat atcctgccct ttgtattcct gcgccagcat caaatccgcc gcgcctgctg
     4201 atgcagggat gaaaaccgcc gtaaaaatcg gtatgatgcc gccgattgtc ttcttaatca
     4261 tctgattccc ccaatatcaa aacgggcggc aaaccgccat aaaacaaacg gcaaacccga
     4321 tgccgtctga aaaaccgttt aggaacacgc cgatgaccct acgttacgaa atcttccccg
     4381 ttaccccctt ccgccaaaac tgcaccctga tttgggacga cgaaagcggc gaagccgtcc
     4441 tgaccgatgt cggcggcgac gtgccgttcc tgctgcaagc gttggcaaac cgcaaactta
     4501 cgctcacggc aatctggctg acgcacggcc atctcgatca cgcgggcggc gtggtcgaaa
     4561 tgttgaaaac gcataaagtc cctgtcctcg ggccgcatcc ggacgatgaa ttcctgctcc
     4621 aatcgctgcc gcaaaccacc gcgcaatacg gatttcccgt ctcgcccgcc tttgcgccga
     4681 accgttggct cgaagaaggc gaaacgctca cggtcggacg ctatgccttt caagtgctgc
     4741 atattccggg ccatacgccg ggacatatcg tcttttattg tgccgaggcg gaattgctga
     4801 ttgcgggcga cgtgctgttt tacgaaacca taggcagaac cgattttccg cgcggcaacc
     4861 acgccgactt aatcaataat atccgcaaca aattattcac ccttcccgaa accgtgcaag
     4921 ttgtcgccgg acacgggcgt atgacttcca tcggacacga aaagcggcac aatccgtttt
     4981 tctaaccgac tttccaaagt cttcagacgg catcccctgc cccgatgccg tctgaaacac
     5041 aaaaggctca ggcaaccgcc gccttgccgg tatacctcgt cagacaaggc tttggtaagt
     5101 acttcaaaca aaccccgaat cagaaccggt acagttacgc cgcgctttcg gcattcccgc
     5161 ccctgctgaa acaagctttt ccgcacaagt cagactgctt catcttctgc cgcatattcc
     5221 aaagattccg acaacgccgt tgtttcattg gcacgttcgg tcaaatcgcg tgcaagctgc
     5281 ttgtttgcct tcacacccaa cagacgcagt ttctcggcgc gtccgaccag attcccgcgc
     5341 ccttcggcaa gttgcttgaa tgccgtctga aaactgcttt gcgcctgatc gatgcctttg
     5401 ccgacgcttt cgagcgtctg tacgaagccg acaaacttgt cgtacagctt gccgccttcg
     5461 tccgcaatcg ccagtgcgtt ctgattttgc tgttcgttgc gccaaatatt cgccaccgtc
     5521 ctcaaagtcg ccagcagcgt actggggccg accagcataa tccgtttgtc gaaacactct
     5581 tggaacaatc ccgcgtcatg ctgcaacgcc agcaggtagg ccggttcgac agggataaac
     5641 ataaagacga aatccaatgt gttcacacct tccaaatcgg tgtaatcctt cagcgacaag
     5701 cctttcatgt gtgcacggat gctggcaacg tgtgccgcca gttcgcgtgc cgccgtatcc
     5761 gcatccgccg cctgcgtgta gcgcacataa gctgtcagcg agaccttgga gtcaatcaca
     5821 atctgcttgt tgtcgggcag gttgaccaaa acgtcgggct ggaggcggcg cgtgccgccg
     5881 tcttcctctt ttcggacgga tgccgcctga accacatatt cccgcccttt ctgaaggccg
     5941 gaattttcca aaaccgtttc cagaatcatc tcgccccaat taccctgaac cttgttctgc
     6001 gtgccggtca gcgcgttggt cagggccttt gcctcgctgt gcagctgcgc attcaatcct
     6061 tgaagccgtt tcaattcgtt ttccaacgtc agccgctcgc gcgattcttt atcataggtt
     6121 tgcttgacca actcgccgaa accgtggatg cgttcgttta gcgggttcaa aacctgatgg
     6181 agctgctcgc ggttctgctc ggtaaaacgg cggctttttt cttccaaaat cgtgttggca
     6241 agattttgaa actgatcgct caagctcttg cgcgcctcgc ccagtaagga cagcttctct
     6301 tcagaagcaa ggcgttcctg ttcgatttgc gttgccaaac gttcgttttc aaccgccaaa
     6361 ccctgtgcct tttcctgcaa ctcggtatgc gactgcctca accgctccgc ttccgcctct
     6421 ttttcctgca aatgggcaat ctgtttttcg gctgcggcaa aacggttgcc gacatcggca
     6481 aggtcgtttt gcacgtcgcg gacagtttgg cggctttctt ccaaatcggt ttcgattctt
     6541 tggcggattt ggcgttccaa ggcatattgg tcggcaaacg ccttccgttc ctgtccgagc
     6601 tgcgttgcca aacgttcgtt ttcaaccgcc aaaccctgtg ccttttcctg caactcgata
     6661 tacgactgct tcagccgaac cgattccgcc tctttttcct gcaaatgggc aatctgcttt
     6721 tcggctgcgg aaaaacggtt gcccaaagca taattttcgt cctgcaaatg tcggtatttc
     6781 ccgtccaaaa cggccaattc cgatgcaatt tctgcgtgtg cctgttcgac aaaatcacat
     6841 cttgccgcct tttccgccag ttgcgcgttc aaatcggcaa actcgccctg aaacctgccc
     6901 ttcatcagca accatgtaaa caacacgccc gacaccaacg ccgccaaagg cagcaaaaca
     6961 gtcatcagtt ccatcaatca tcctaatatt caaacatttt cacaccggac aaaaccgccg
     7021 cttattccga ttctacctgt ttgttcgaca tagctccaaa aaatatagcg gattggcttt
     7081 aaacctgttc gacatcgcct taccatgctg cttgcggttt cagacctttt cctaattcaa
     7141 tatcaatctg ccacaaaccc tgattaagtt cccgatgtct gacattttta gaatgatgcc
     7201 gtctgaaatg ttgcagctat gttcagacgg catacggatt taggcttttc aaacggcagg
     7261 caaaatgaaa aaagggcaaa ccctaaagga tttgcccttt tgttccaaac gcttagtgta
     7321 cgtctttcca atattctttt ttcaggaaat acgccaaagg cagcataacc gcaaatagga
     7381 aaatcatcac gacatagcct atacgtttgc gttgcagttg tgcaggttcg cccatgtaca
     7441 caaggtaatt gaccaaatcg cgtacatatg cgtcgtactc tttttggatc actttgccgt
     7501 taggcagacg gcggctgtgc aaaccggtag attcccaata cagcttaggc ttcatctcgc
     7561 cgtgttcgtc ttttaccata accggctgac ctttggcatc caactcaacg gcttgaacgc
     7621 cttgctgttc ccacaacggg tggggcatac cgactttatc gaatacagta ttgttccagc
     7681 cgctcggacg ggtcggatct ttatagaagc cgcgcatata agcgtaaagg tagtctgcac
     7741 ctttggaacg cgcaatcaat gtcaaatcgg gcggagcagc accaaaccat tttgccgcat
     7801 ctttcgggtt catcgccgaa tgcatgacat cgccgacatt atcggtggta aacatcaggt
     7861 ttttcttgat ttcttcgtca gtcaaaccga tgtctttcaa acggttgaaa cgcataccgc
     7921 ttgccgagtg gcaggacagg caatagttgg taaagatttg tgcgccgcgc tgcaggctga
     7981 cttggtcgcg caaatcgata tcgactttct catagtgtgc atgaccgccc gaagcaaatg
     8041 ccgcacccat gggtactgcc aacagcaagg cagcaaacca atttttcata gcttgtttca
     8101 ttttgactgc cctcatcaga tgttggttgc aaacaggtaa gcacccacgg cggtaatgcc
     8161 gacgtaaaca aagaacatga ttttttgttt ggcggtgctc atggttacgc gttcgggaac
     8221 cggtttgttt ttatccagct tggtatagaa cggcataccc aggaagaatg cgaaatagac
     8281 gaaggacagg atgcgcgcga ccaaagtacg cgtatcggtt gccaccatcg cgcccaaaat
     8341 acccaaaccg ataaaggaaa tgatgaacaa gaccaatgcg gttttgaaaa tcgggccgcg
     8401 gtaacggacg gatttcacct cgcctctatc cagccaaggc agcagggcaa tcaacacgac
     8461 cgccgcgccc ataccgatta caccccaaac ctgcgttccc aagaacgagg gaatcgcacg
     8521 caaaatggcg tagaacggtg tgaaatacca taccggcgca atgtgcggag gtgttttcag
     8581 cgcatttgcc gcatcgaagt tcggcgcttc gaggaagtag ccgccgcctt ccggtgcgaa
     8641 gaacaatacg gcacagaaga caatcaggaa tacaaccaca cccaagatgt ctttaacggt
     8701 atagtaagga tggaacggga taccgtcgcg cgggataccg ttttcgtctt tgtttttctt
     8761 gatttcaacg ccgtcagggt tgttagaacc cacttcgtgc agggcgatga tgtgcgccac
     8821 aaccaagccg agcaatacca gaggtaccgc gataacgtgc agggcaaaga agcgattcag
     8881 ggtaacgtcg gaaacgttga agtcgccgcg aatccaagtg gacaaatccg gaccgataac
     8941 agggatggcg gagaacaggt taataattac ctgcgcaccc caaaaggaca tctgacccca
     9001 aggcagcagg tagcccataa aggcttccgc catcaatgcc aagaaaatca gagaaccgaa
     9061 aatccacacc aactcacggg gttttttgta cgaaccgtaa atcaaaccac ggaacatgtg
     9121 cagataaaca acaatgaaga agaaagatgc gccggtggaa tgcatgtagc ggataatcca
     9181 accgccggac acgtcgcgca tgatgtactc gactgcagta aaggcagcag gcagatggta
     9241 ggcattcagg ttaccgtccg gtttgtagtt catcgtcagg aaaataccgc tgacgatttg
     9301 aatcacgagg acaagcaagg ccaaagagcc gaagtagtac caaaaattaa agtttttcgg
     9361 tgcgtagtat tgagccaaat gctcattcca cattttagac agggggaaac gagcgtccat
     9421 ccagcctaac aatgcttttg ctttgctatt ggtttggttt gccataatta tcgttcctta
     9481 ttcttagtct tcgcccacca agatagttgt gtcgctcaag tatttatatg gcgggacaac
     9541 caggttggtc ggggcaggaa cacctttata tacgcggccg gccaagtcga atttcgaacc
     9601 gtggcagggg cagaagaagc cgcctttcca gtctgcaccc aaatcggcag gggcaatgtc
     9661 gggacggaag gtgggcgagc agcccaaatg ggtgcagata ccgatggcga caaggatgtt
     9721 cggcttaatc gaacgggtct cgtttttagc atactccggc tgctgttccg catcggaatt
     9781 gggatcggta agttcgccgt tcaggccttt caggtcttta agctgctgat ctgtacggtt
     9841 gagcacccaa atcggtttgc cttgccactc ggcggtcagc agctgacccg cttcgatttt
     9901 actgacatcc acctcgacgg cagcaccggc ggccttggct ttttccgaag ggaaaaaact
     9961 ggccacaaac ggcgttgcca cacccaatgc tgccactccg cccgcgccgc aggtcgcgag
    10021 tgtcaggaaa cggcggcggc cgttgttgat ttcttgatta tccattattc agtcgtccta
    10081 atattttggg aataccgagc cattaaacgt tgcaatttta cccagtttgc agtgatactc
    10141 aaagcattat ttaaaataag gtaaagtttt atgatatttc tcaagactca agccggattg
    10201 ttttcgtcaa aatggcacac ttccaacccg aaaacctctg ccgccgattc tgccagcgcg
    10261 cgtacgccgt aacgttccgt cgcgtgatgc cctgccgaaa tgaaagccgt acccgtttca
    10321 ttggcaaggt ggtattgagc ttcagagatt tcccccgtca aatacagatc gacaccttcg
    10381 tctattgccg tctgaaaaaa cccctgcgcc ccgccgctac accatgcaac ccgtcggatt
    10441 tcgcgttcgg gtttgccgat gacgacaggc ttacgttgca aaactgtttc aatatgcgcc
    10501 gccaatgcgc cgagtgtctt ggcttgtttc aggctgcccg agttgagcag gttttgttcg
    10561 ccgaaccgtt tttctgtcgc aaaacccaat ctgtcggcga gttgggcatt gttgcccagt
    10621 gtgggatgtg catccagggg cagatggtag cctgccatat tgatgtcgtg ccgtaacagt
    10681 gcggcaatcc gttctttttt ccaaccagta acggtcggca actcgttttt ccagaacata
    10741 ccgtgatgta ccaaaagcaa atctgccttc tgctccacag caaaatcaat cgctgccctg
    10801 cttgccgtta ccgacgtaac gattttcccg atatattccc tcccttcaac ctgcaaaccg
    10861 ttaggggcgt aatctttaaa caacgctgtc tgcaatgttt cattacacca agtcagaaaa
    10921 tccctgcgca ataccatctt ttttcctaat cgctttaaac aagcgggcat tctaatcgca
    10981 aaatgtccgg aattcacatt tttccgattt gcacccgcat atgaattatt ttaatatgcg
    11041 ccggttcaat atgccgtctg aagccccatg gattccatca tcgaactgcg ccacctcaag
    11101 accctgctgg cacttgaaga aaccggcagc gtctcccttg ccgccaaacg ggttttcctt
    11161 acccaatctg cactttccca ccaaatccgt atgcttgaaa accactacgg cgcaccgttg
    11221 ttcgaacgaa aatccacgcc cttgcgcttt accccggtgg gcgaaaggct gctgcgcctc
    11281 gcccacgaac tgatacctca agttgctgtt gcagaacgag atttggcgcg aatcacggaa
    11341 ggggaggcgg gagagctgcg gattgccgtc gaatgccata cctgtttcga ctggctgatg
    11401 cccgccatgg gcgaattccg cccgatgtgg ccccaagtcg aactggatat cgtatcggga
    11461 ttccaagcgg atcccgtcgg gctgctgctg caacaccgcg ccgaccttgc cattgtttcc
    11521 gaagcggaaa aacaaagcgg catcagcttc caaccactgt ttgcctacga aatggtcggc
    11581 atctgcgcca aagatcaccc gcttgccgcc aaaactgtct gggaagccga agactttatc
    11641 ggggaaaccc tgattactta tcccgttccc gacgagatgc tggatttgcc caaaaaaatc
    11701 ctgattccga aaaacatcaa cccgccgcgc cgacacagcg aactgaccat cgccattatc
    11761 caactggttg ccagcagacg tggcattgcc gcccttccct attggacagt catgccctac
    11821 cttgaaaaag gctatgtcgt ccaccgccaa attactgccg acggactgca aagcaaactg
    11881 tatgccgcca tccgtaccga agatacggac aagagctatc tgaacaattt ttgccaaatc
    11941 atacgcgaac gcggttttgc agatttgccc ggactgagcg aactggaacc ggtctgaccc
    12001 cttattcaac catacccggc agtttttcta ttttttcatg tatagtggat taacaaaaac
    12061 cagtacggcg ttgcctcgcc ttgccatact atttgtactg tctgcggctt cgtcgccttg
    12121 tcctgatttt tgttaatcca ctatactgtt tttgattttt gcccaatctg taatctttag
    12181 attgccaatg ggaaaccgtc tactacaaat aaaaaaccct gcgataagca gggttttttg
    12241 aatttccaac attaacgttt ggagaattgt tttgcacggc gtgctttgcg cagacccggt
    12301 tttttacgtt cgacttcgcg ggcatcgcgg gtaacaaaac cagcttgaga caaggcgggt
    12361 ttcaacgcgg catcgaagtc gatcagggca cgggtaatgc cgtggcggat tgcgccggac
    12421 tggccggttt cgccgccgcc aacaacattg actttgatgt cgaaagattc ggcgttttca
    12481 gtcagaacca agggttggcg aacaaccatt cggctggttt cccgtgcgaa gaattcgtca
    12541 acgggacgac cgtttacgat gatttgacct gtacctttaa tcaggaatac acgagccact
    12601 gaacttttgc ggcggcctgt gccgtagtag tatttaccgt tcatgtcgcg tccttatttc
    12661 agttccaaaa ctttgggttg ttgcgcagca tgggcgtgtt ccgcacccgc atacactttc
    12721 agttttttaa tcatggcgta acccagagga cctttgggca gcataccttt tacagcttgt
    12781 tccaaagcgc ggcccgggaa ttgctcttgc atttcgcgga aggtgcgttc gtagataccg
    12841 cctgggaaac cggaatggcg gaagtatttt ttatcttcga atttggcacc ggttacacgc
    12901 agtttgtccg cattgataac aatgatgtaa tcgccggtat cgacgtgggg ggtgtattca
    12961 ggtttgtgtt tgccacgcag acggctggcg acttcggccg caacgcgacc caagactttg
    13021 tcttgggcat cgatgacgaa ccattcgcgc ttcacctcgt ggggtttcgc tgaaaaggtt
    13081 ttcatagtgg aaatccagat agatatagaa agttgtaaat tttaaagaca ggattcgatt
    13141 ttgtcaatcg cattaccgcg ttacggaagg attttccgga tttcggcaga ctgcatactg
    13201 ctttttcggg cggggcggcg gccaatgtga aaaaccgcat cgttgcgatg cggttttgaa
    13261 tgggaatccc cgcgagagcc gtttcggccg aatccgcttg aaccttgctg acaaggcggc
    13321 tgcctcgggt agtttcgggt gcgtccgcaa aaggacgctc gcgcccacta ctgctcccgg
    13381 caaccttaag cgaacttatt ggttcaaagg aatatatgcc ttcgcggaca ccgcagggaa
    13441 aaaggggtta ttcctgcgcc aagcgggata gtgctttttg gcaggcgttg tccatatcgg
    13501 ctattttacg cgcaaaatcg ccgattgcca aatcgccgcc gttcagggag gttttcaaca
    13561 ggtcgtggac gacgttgagc gcggccataa tgacgatttt ttcgctgtcc gcgacgcgtc
    13621 cgccttcgcg gatggcttcg gctttgccgt tgagcattcc gactgcctgc aacagtgtgt
    13681 ctttttcttc tgccggcgtg ttgacggtca gccgggcgtg catgacttcg atgtggactt
    13741 gttcgatgtt catcctttaa tccttattgc tgcgtttcct gccattgggg gaggcgcgct
    13801 gccagtgcgc tgattttttc cctgctctgt tcgagcaggc tgcggtatcg tgtattttct
    13861 tctgtcaggc tgtcaatttt gttttgcagg tcttctttga gtttgccgac ttggacgagc
    13921 agggcttcgc tgagttcgtc gacggcggtt tcgtgttcga gtttttgccg ctcgtgcgcc
    13981 cgtttgagtt cggcgacggt ttctttgagg cggcggtttt cgctgacgag ggtttcgaat
    14041 ttttgtacca acgtataaac gctgctttcg agtttttcga tattttgttt cataacctta
    14101 cctgtccgta tgccgtctga aggcttcaga cggcatctgt ctgttgttta ttcaaaacgc
    14161 gcgctgcgtt ccatcagtct ttcgacaacc tgttgcgggg tcatttcttt gcggatgagt
    14221 tgcagcagag tttgggtaat cggcatgtcg atttggtact tacaggcagt attgaagact
    14281 tcttctatcg tgctgacccc ttcggaaacg tgtccgattt cgaccagcac ctgatgcagt
    14341 tccttgcctt ctgccaaacc caagccgacg cggcggttgc gcgaaagtgc gccggtgcag
    14401 gtgaggatga ggtcgccgat gcctgccagc cccatcatgg ttttgggctg tgcgcccatt
    14461 gcggaggcaa ggcgggtgat ttcagctaat ccgcgcgtaa ccagtgcggc acgggcgtta
    14521 agcccgtact ctaggccgtc ggacaatccg gtggcaatcg ccataacatt ttttaccgcg
    14581 ccgccaaccg ccacgccgat aacatcggta ctgccgtaaa gcctcatgac ggtcgtgttg
    14641 agctgcggta cgagttcttc aatccactct tggttttcgg aggcaaggac gacggcgcag
    14701 ggcagttgtt tggcgagttc ctgtgcaaaa ctcgggccgg aaagtacgcc gattttctta
    14761 ttgtcgggca atacttcttt caagacttga aaggtcagca gcccggtatc ctgctcgaat
    14821 cctttgcagg cggcgaggac ggggaggtgt cccgcgccgt actgtttgag cagctctgcg
    14881 ctgcttctca atccggcaac ggaggttacg ataaggacaa gtccgctgtc tttgagcgcg
    14941 tctgccaaat ccgcacacac ttccaaggtt tcgggaaagg aaaagccggg cagtccgcgt
    15001 ttgttttcac gcgcttcctg catttgacgg acttggtctg cgttgcgcgt ccacagggat
    15061 acgcggttgc cgtgttggga aaaatgcagg gcgagcgccg taccccacga acctgcgccg
    15121 ataacggtaa ttttcattgg tcgtctttca acatatcact gccgttcact ttaaaacaat
    15181 cggtgtttct ctgcaagtgc ggtcagggaa atgccgtctg aaaggcgttc agacggcatt
    15241 ttgccccgat gcggcactat cagcctgtat tgcgcaaacc ttgcgccacg ccgttgatgg
    15301 tcaggtgcac catcagaagg gcgtgcggat tgtcgggttc tttacgcagg cgtttgagca
    15361 tggcgacttg caaaccgttg agcgcgttca ggtagggaat cctcaaagcg agcgaacggg
    15421 cgaggctgcg gttgtcgcgc aaaagctctt cggtttgcag taggtcgagc agtgctttgc
    15481 ggctgcggcg gtattcttcc ttaatcatcc cgaagatgat ttttgcctta tcgggcgatt
    15541 cgctcaagcc ggcatagttt tccgcgaggg tgatgtcggt tttcgccatc acttgttcca
    15601 tattggagag catggcttgg aagaacgggt tgctttgggc gtgttcgcgc agggcggcga
    15661 gcgtttcggg tttgtcttcg cacaaggttt ccaccgcgct gccgaaaccg taccaagccg
    15721 gcagcatgag gcggttctgc atccaggaaa atacccacgg aatcgcgcgc aagtcctgaa
    15781 tccgcgccaa ggttttgcgg ctggcgggac ggctgcctag gttgagggtg gcgatttcct
    15841 gaatcgggct ggtttgcaga aagtagtcga tgaagtcggg atgggtaatc agttcgcggt
    15901 agtatttgaa cgatacgtcc gacaatgcct gcatcagttt ggcatcaggg tcttttttat
    15961 ccggcaggat gctggcttcc aaagtcgcgg caaccaaggt ttccaagttg cgttgggcat
    16021 tgccggggtc ggcgtatttg gcggtaatga cttcgccttg ttcggtgatg cggatttgtc
    16081 ccgccacgct gcccgccggt tgggcgagaa tggcttggta agaagggccg ccgccgcgac
    16141 ctacgctgcc gccgcgtccg tggaacaggc gcatacggac atcgtatttt ttgaagagtt
    16201 cgaccaagcc caattccgcc tgatagaggc accatgagct ggtaacgtag ccgccgtcct
    16261 tgttggagtc ggaatagccg agcatgattt cttggatgtt tccacggctt tcgagcagtg
    16321 catcgtacca gtcgaggcgg aacatggttt ccatgaccgg acaggcgttt tccaacgctt
    16381 caatggtttc aaacagcggc acgatattga tgcggctgtg cggtttgccg ttttccaccg
    16441 ccaacaggcc ggtttctttc agcagcaatg ccaaggcgag caggtcgctg ggttgttcgc
    16501 agttggaaat aatgctttgt gttacggcat cttcgccaaa ttcgtctttg attttgcgcg
    16561 cttcgttgaa aattgccagt tcgtggcggg tatggtcgct gtatgtgata aacgggctgt
    16621 acagaggacg ttgatggctc aattcgcgca acagggcggt ttgtttttgc tcttcgttca
    16681 ggcggttgta gtcttccaag cctgcgtgtt ggaaaagctc ggcaaccaca tcggcgtgtt
    16741 tgcctgcgtg ttggcgcaag tcgagcggca tcatgtgaaa gccgaacacg gatacggaac
    16801 ggatgaggtc tgccaaacgg ccttcggcaa gcagacggct gccgttgtcg ataagggaac
    16861 gttgcaattt tttcaaatca tccagaaact cttgtgccga agcataaggc tcgagaaagc
    16921 cgaatttgca gcccataccc aaaccgagcg cgcgcgcttt gcccatagcg cgcgccataa
    16981 tgtaggcgat ggcgcggcgg tagggttctt cggcgcgggc gatttcttcg tcgggcgatt
    17041 tgtcggacaa cgccgttaca tcgccgttga ctttgacgcg gcggatggag agcggcagtt
    17101 cgcggtagag tttgtcgagt tcgccgcgat agaagcggaa cacggcatcg gcgtggcggc
    17161 ggaaggcaaa gcgcagggtt tcggcagaaa caaacggatt gccgtcgcgg tcgccgccga
    17221 tccagccgcc gattttgagg atgtccggaa cgcggacgcc gggataggcc gtctgaaagt
    17281 cgtgttccat cttgcggtag agcttgggca gggcttcgaa aaagctcatc gggaagatgg
    17341 acacgccgtt gttgatttcg tcgttgacgc tgagtttgtg gcggcgcgtt tcgctggtct
    17401 gccacaagcc cagcaggata gtgtcgattt cgcggcgcag ccgtgccagc gcgtcggcat
    17461 tggtgcagcg ttcgcgttgc ggcaacagtg cgcggatgcg gcggttgaag cttaagacgg
    17521 tttggcgttg cacttcggtc gggtgcgcgg tcaaaacggc ggtaacggac gtattgtcca
    17581 actgccgctg caccgatttg ccgtcggctt tccccgcttt gagcctgcgg acggtttccg
    17641 tcaggctgcc ttccgcgccg ccgcgtccgg cttcttcgtg gatttggcgg cggcgttcgt
    17701 ggtgcacgtc ttcggcgatg ttcaaaatct gggcgaacag gccgcaggcc aaggttaaat
    17761 cgtgggtttg ttgttcgtcc aattgcggca atactttttc aatcaatgcc gcgctgtcgt
    17821 cggaagtgga caagagtttg actgtttcga caaccaacgg cgaggcttct tcgtgcagga
    17881 ggttgaacag ggattgtttc agaaattccg cgtccgccgc caaagccgcg tcctttggat
    17941 tgttcagaat atgcagttgc atgatttttc tctctcgtct gccgtaaata ttgtaaatgt
    18001 accccaaatg ccgcatccgt gccaaaccgt tcacacttta accgcccgtg tcccgaaatg
    18061 ccgtctgaag ttgaacgccg cccgacggca gcgttacaat cgcccgcaac tgtttttttc
    18121 cgaacatcat catgaccacg accgaacacg acaacgacga tgcattcctg ctgcggtaca
    18181 gccgccacat cctcttggac gaaatcggca tcgaagggca gcagaaactt tccgccgcgc
    18241 atattttggt cgtcggctgc ggcggtttgg gtgccgccgc actgccctac cttgccgctt
    18301 cgggtgtcgg cacgctgacc atagccgatt ccgacacggt cgaactgcac aacctgcaac
    18361 gccaagtcgc atttgacgag ggcgatgtcg gcaaactcaa aaccgaagcc ttggcaggcc
    18421 gcctgaaacg catcaaccat accgtcaacg tccgcgccgt caacgaaaaa ctcgacggct
    18481 gccgcctgac cggtttggtt caagccgccg acatcgtttt agactgttgc gacaactacg
    18541 ccacgcggca agccgtcaac cgtgcctgcg tgcaaacgaa aacaccgctg gtttcagggg
    18601 cggcggtacg ctttgaaggg caacttgccg tgtaccgtcc cgacttgccc gactcgccgt
    18661 gttacgcctg cctgtttgac ggcggatcgg cttcagacgg catctgttct ctcttcggcg
    18721 tgttctcgcc gctggtcggc atcatcggca gtacccaagc ggcggaggct ctgaaaatcc
    18781 tgctggatgc gggcgaaccg tcgcacggca ggctggcggt ttaccgtgcc ttggaagggg
    18841 gctggcaata tttcgacctg ccgcgcaacc ctgaatgccc ggtttgcggc acagcgcgat
    18901 aaaccctgcc gccgtttcag acggcatcca aacggatgcg gcggaacggt tttaaaaatt
    18961 taaaaattta catttctttg caaaaaaaaa aaaaaaaata aacttacctt ataattgcaa
    19021 ttgttttagc aatgtctgtt tcgcagactc attgagtaaa acgttttccc cgtaatgtgt
    19081 ttggccgtct gtcccctttg ggttcggacg gctttttttt ggctgtgttt gaatacccgg
    19141 ttggttttat ctgtttgcag cgggggaagc cgcttatttc cgttcgggcg gaaaacggtt
    19201 ccatcggata aaaggcattt tgtccgactg attaaa
//
LOCUS       DAWGRZ010000020        18797 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000020 DAWGRZ010000000
VERSION     DAWGRZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.020"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(49..2886)
                     /locus_tag="WHT44_000494"
     CDS             complement(49..2886)
                     /locus_tag="WHT44_000494"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ2532721.1"
                     /translation="MLDKYNPAEIESKHYQNWEEQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFNLEAKVLANAEVFNFKGEAQLGFALPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKPHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDNEG
                     NVYVARNQEEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLEKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADSGEIVQTAFEQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESTDDLADYLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(2983..3792)
                     /gene="zupT"
                     /locus_tag="WHT44_000495"
     CDS             complement(2983..3792)
                     /gene="zupT"
                     /locus_tag="WHT44_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ2532722.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTTGMAVIAVSLVLFHF"
     gene            complement(3973..5229)
                     /locus_tag="WHT44_000496"
     CDS             complement(3973..5229)
                     /locus_tag="WHT44_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ2532723.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWQMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEGRKKGTLQIFRQTKEVEAAKQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQNISRIDHNGLRIKTVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(5308..6699)
                     /locus_tag="WHT44_000497"
     CDS             complement(5308..6699)
                     /locus_tag="WHT44_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ2532724.1"
                     /translation="MQVFLDNPKAFFETVSGWVWGPLMLMLLVGTGILLTVLLKGLQF
                     TMLGYALKQAFMPPKKHKSGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(7056..7832)
                     /gene="lpxA"
                     /locus_tag="WHT44_000498"
     CDS             complement(7056..7832)
                     /gene="lpxA"
                     /locus_tag="WHT44_000498"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ2532725.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVIGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSARGIIR"
     gene            complement(7919..8368)
                     /gene="fabZ"
                     /locus_tag="WHT44_000499"
     CDS             complement(7919..8368)
                     /gene="fabZ"
                     /locus_tag="WHT44_000499"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ2532726.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAIIMCAKRVV"
     gene            complement(8402..9448)
                     /gene="lpxD"
                     /locus_tag="WHT44_000500"
     CDS             complement(8402..9448)
                     /gene="lpxD"
                     /locus_tag="WHT44_000500"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ2532727.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPTSCEIGANVYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFADDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            complement(9481..9981)
                     /locus_tag="WHT44_000501"
     CDS             complement(9481..9981)
                     /locus_tag="WHT44_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ2532728.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(10047..12440)
                     /gene="bamA"
                     /locus_tag="WHT44_000502"
     CDS             complement(10047..12440)
                     /gene="bamA"
                     /locus_tag="WHT44_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ2532729.1"
                     /translation="MKLKQIASALMMLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(12497..13837)
                     /gene="rseP"
                     /locus_tag="WHT44_000503"
     CDS             complement(12497..13837)
                     /gene="rseP"
                     /locus_tag="WHT44_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ2532730.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLNYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(13872..15107)
                     /locus_tag="WHT44_000504"
     CDS             complement(13872..15107)
                     /locus_tag="WHT44_000504"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ2532731.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFHPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDRITPA
                     QAVKHPNWRMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLRL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLAQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(15112..15909)
                     /locus_tag="WHT44_000505"
     CDS             complement(15112..15909)
                     /locus_tag="WHT44_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ2532732.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSKLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(15912..16658)
                     /locus_tag="WHT44_000506"
     CDS             complement(15912..16658)
                     /locus_tag="WHT44_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ2532733.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(16714..17271)
                     /gene="frr"
                     /locus_tag="WHT44_000507"
     CDS             complement(16714..17271)
                     /gene="frr"
                     /locus_tag="WHT44_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ2532734.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(17451..18578)
                     /locus_tag="WHT44_000508"
     CDS             complement(17451..18578)
                     /locus_tag="WHT44_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ2532735.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHAVRLGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWLDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLADCSKMIAEISNGRRMTRERLEENMAKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(18654..18729)
                     /locus_tag="WHT44_000509"
     tRNA            complement(18654..18729)
                     /locus_tag="WHT44_000509"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18694..18696),aa:Thr,seq:cgt)
BASE COUNT         3792 a         4876 c         5567 g         4562 t
ORIGIN      
        1 aggccgtctg aaaaacattt ttcagacggc cttgtttatt caatcaaatc agtctttcaa
       61 cttcgccaac tgattttgaa cttttgccat tttgtcttcc aattccgcca aatcggcttt
      121 gtctttttcc accagatgcg caggggcttt ttcggtgtag ccgggtttgg agagtttggc
      181 gttgagtttg tccaaggctt tttgcagctt ctcggcttct ttgctcaaac gggcggtttc
      241 ggcggctttg tcgatttcga ctttcaacat caggcgcgcg ccgttgcaga cggcgacggg
      301 cgcgtcttcg ctttcgggta gggcggcgac ttgctgtgct tcggtcaggc gggtcatcat
      361 cggcaggtat ttgaggtagt ccgccaagtc gtccgtgctt tcgacaaaca gcggggcttt
      421 tacgttgggc tggatgccca tttcgccgcg caggttgcgg actgcgccaa tcaaatcctg
      481 caacacggtc atttgctcga atgccgtctg aacaatctcg ccgctgtcgg cttcggggaa
      541 gcgggcgagc atgatgctgt cggcggtttt cgcgtcgcac ataggagcga cggtttgcca
      601 cagttcttcg gtgatgaacg ggataatcgg gtgcagcagg cgcagggcgg cttcgagtac
      661 gcgcaataag gtatggcgtg tggcgcgttg gcggctggcg cagccggttt gaagctgcac
      721 tttggcgagt tccaaatacc agtcgcaata gtcgttccat acgaagctgt acagggtttc
      781 cgccgccaaa tcaaagcggt aggtttcgta ggcttgcgta acctgttcga tggtctgatt
      841 cagacggcct acaatccaca tatcggggaa ggagtagccg cgcggttcgg cagcggttgc
      901 gccgtaaccg cagtcttggt tttcggtgtt catcaagacg aagttggtgg cgttccagat
      961 tttgttgcag aagttgcggt agccttcggc gcgtttgaag tcgaagttga ccgaacgccc
     1021 caagctggcg tagctcgcca tagtgaagcg caaagcgtcc gcgcccatac tcggaatgcc
     1081 ttcggggaag agttttttcg tggcttcttc cactttcggc gcggtttcgg gtttgcgcag
     1141 gccggtggtg cgttttacca gcagtttttc caagccgatg ccgtcgatca aatccacagg
     1201 gtcaatgacg ttgccttcgg atttggacat ttttttgcct tcgtggtcgc gcacgatgcc
     1261 gtggatgtac acgtctttaa acggtacttt gccggtgaag tgggtggtca tcataatcat
     1321 acgcgccacc cagaagaaga tgatttcgta gccggttact aagacattgg acggcaggaa
     1381 ggctttgagt tcgtcggttt cagacggcca gccgagtgtg gagaacggca caagcgcgga
     1441 ggagaaccat gtatccaata cgtcttcttc gcgagtcaag cctgttttgc cggcttgttt
     1501 ttcggcttct tcctgattgc gggcaacata cacattgcct tcgttgtcgt accatgcagg
     1561 gatttgatgg ccccaccaca gttggcgtga gatacaccag tcttggatgt tgttcatcca
     1621 ttggttgtaa gtgttgaccc agttttcagg gataaagcgt accgcgccgc tatcaacggc
     1681 ttttttggct ttatcggcga ggctcaagcc tttgaactcg ctgtccggct cgccgccgtt
     1741 tggggtggcg gacatggcga caaaccattg gctggtcagc ataggttcaa tcaccgaacc
     1801 tgtacggtcg cctttcggcg tcatcagcgt gtgtggtttg atttcgacca agaaaccttg
     1861 ttcctgcaaa tcggcaacca tttgtttgcg cgcggcaaag cggtctaagc ctgcgtattt
     1921 ttcaggcagg gcaaagccta gttgcgcttc gcctttgaag ttgaacactt cggcgtttgc
     1981 cagcactttg gcttccaagt tgaacacatt aatcaggcgc gtgtcgtggc gtttgccgac
     2041 ttcgtagtcg ttgaagtcgt gtgcaggcgt gattttcacg cagcctgtgc cgaagtcttt
     2101 ttcaacgtat tcgtcggcaa tcacggggat agtacggccg gtcagcggca ggattaattc
     2161 cttgccgatt aagtgggtat aacgttcgtc ttcaggattg acggcaacgg caacgtcgcc
     2221 cagcagcgtt tcaggacggg tggtcgccac gataacggct tcggcgggat tgtccgccag
     2281 cggatagcgg atgtgccaca tagagccttg ttcttccacg ctttccactt ccaaatccga
     2341 taccgccgtg ccaagcacgg gatcccagtt caccaagcgt ttgccgcggt aaatcaagcc
     2401 ttgctcatac aggcgcacga acacttcggt tacggtttcg gcgcgcacgt cgtccatcgt
     2461 gaaatactcg cgcgtccagt cggcagagca gcccacgcgg cgcatttgtt gggtaatcgt
     2521 gccgccggaa acttctttcc attcccacac tttctccaaa aatttttcgc gacccaagtc
     2581 atggcgggac acgttttgcg cagcaagctg acgctcaacc acaatctgcg tggcgatgcc
     2641 cgcgtggtct gtgccgggaa tccaggcggt gttgcagcct ttcatgcggt agtagcgggt
     2701 cagaccgtcc ataatggttt ggttgaaggc atgacccatg tgcagcgtgc cggttacgtt
     2761 gggcggcggc agttggatgg agaaagacgg tttcgtcaaa tccatatcag gttggaaata
     2821 gccctgctct tcccagtttt gataatgttt ggattcgatt tcggctggat tgtatttgtc
     2881 taacatgatg gaactttgtg aaattaaggt tatttttgat gtgcggatta taacgcaaaa
     2941 aggccgtctg aatcatttca gacggccttt ggcatacagg ttttaaaaat ggaacaatac
     3001 caggctgacg gcaatcaccg ccatacccgt tgtcaggccg taaacggttt catggccgtc
     3061 tgaatagcgt ttggcagccg gcagcagctc gtccaacgcc aaaaacacca tcacaccggc
     3121 tatcacgccg aataccgaac caaacacggc aggcgacaaa aacggctgca aaaccaaata
     3181 gcccaaagcc gcccccaacg gctcggccaa gccggatagc agacacgccc acaccgtttt
     3241 cttacggctg cgggtggcaa aataaaccgg cgcggcgatg gaaatgccct ccggaatatt
     3301 atggatggca atcgccaagg ccaaaggcat cccgactgct ggattttcca atgtggcaaa
     3361 aaacgtcgcc aagccttcgg ggaaattgtg cgcagtaatc gcaaacgccg ccatcatgcc
     3421 gactcgcgcg atatggcggc gtttgctttc ttgaaacgac gggtcttgcg cgtctaaagt
     3481 ttcatgcggg ttcggcacca gacggtcaat cagcgcaatg ccgcccatcc cggccaaaaa
     3541 tgccatggtc gccgccgcaa acgcgtggtc tttatcataa atttcagcga acgcctcgct
     3601 ggacttactg aaaatctccg tcagggaaac atataccatc gcaccgccgg caaacgccaa
     3661 accaaacgac aacacacgcg gattgggcgt tttggaaaac atcaccaagc cactgcctaa
     3721 tacggtaaac aaaccggcag ccaatgtgat ggaaaaggca acggccaaat tggacatcga
     3781 aaaatcgggc atgagaaaac ctgcgctaaa agctgggaca ggttcagact aacacttttt
     3841 aatgtatatg ataatagtta ttatttattt tattgattgg atacacggat tttgaaacaa
     3901 aaggccgtct gaaaaatgat tttcagacgg cctttaaatt tgaaatgccg ctaaacctta
     3961 gtgctttcca gcttaagcct gataacgcga caggctcaaa tcgtcgctgc ggatttcggt
     4021 gtctttgccg ctcacgatat cggcggttaa ttttgccgaa cccagcgaca tggtccagcc
     4081 taaagtaccg tggccggtat tcagaaacag gttgtcaaag cgggtgcgac cgattaacgg
     4141 cgtgctgtcg ggcgtcatcg gtctgaggcc gctccagaac gatgcttggc tcaaatcgcc
     4201 gccttccggg aacaagtcgt tgacgaccaa agccaaggtt tcgcggcgtt tttcgggcag
     4261 tttgatttcg tagcccgaca attccgccat accgccgacg cggattctgt tgtcaaagcg
     4321 cgtgatggcg actttgtagc tttcatctaa aacggtggac accggtgcgc cgtctgaatt
     4381 ggtgaccggc agggtcaagg aatagccttt gacgggataa atgggcagat tgagatccaa
     4441 ctgcgccaaa accgtcctgc tgaagcaacc gagcgcgcag acaacggcat ctgcttcaaa
     4501 ccgccctgtt tcggtttcaa cggttttgat gcgcagcccg ttgtggtcga tgcggctgat
     4561 gttttggttg aaatgaaacc gtacgccctt ttcctgacac aatttgtata ggttttcagt
     4621 gaagaggcgg cagtcgccgg tcgcatctgc aggcaggtgc aggccgccgg caattttggc
     4681 ggtaacgcgt gccagcgcag gctcaaattc tgcacattct tcgggtttca gacggcggta
     4741 cggcacgccg tagcgttcca aaacggcaat gtcttgtttt gccgcttcga cttctttggt
     4801 ttggcggaaa atctgcaacg tccctttttt gcgtccctca aaattcatgc cggtttgcgc
     4861 ttcaaaacgg cggaacattt cacggctgta ttcggaaatc ctgaccatgc gctctttatt
     4921 ggtttgatag tgcgctgccg tgcagttttg cagcatttgc cacagccatt cgatttgata
     4981 caggctgccg tcggggcgaa acagcaaagg cggatggctt ttaaacagcc atttcagcgc
     5041 tttggtcggg ataccgggtg cagcccaagg cgtggtatag ccgtaagaaa gctggcctgc
     5101 gttggcaaaa ctggtttcca tcgccacacc ctcggcgcgg tcgatgaccg ttacttcatg
     5161 tccggcctct gccagatacc acgcggaaga cacgccggca acacccgcac ctaaaacaag
     5221 cactttcatg tttctccctc cggctttttc aaaacagact taatatgccg tgccgtctga
     5281 atattcggat tcagacggcc tcggatatta atgcggcaat tcgccgtttg tgattttttg
     5341 tttgaagtcg cgcgtttcat tgacgatgac tttcgccatc aataaaagtg caatcaggtt
     5401 gggcaatgcc atcaagccgt tgaatgtgtc cgaagccagc cacaccaaat caaggctcaa
     5461 cacggtaccc agcataacgg aagaaacata acccacgcgg tacaaaccgg caaatttctc
     5521 gccgaaaaca tacaccgcgc atttttcgcc gtaatagcac cagcccaaaa tggttgagta
     5581 ggcaaagaaa atcaggccga tggtaacaat ccagccgccg atgccgggca gcattttttg
     5641 gaatgtgacg gttgtcagtg ccgcgccgct cacttcaggt ttgacaaact cgccgcccgc
     5701 gccgagcagt cccattacca acacgatgcc ggtaatcgag caaacgacga tggtatccaa
     5761 aaacgtaccg gtcatagaaa ccaaggcctg acggacggga tggtcggttt tcgcggctgc
     5821 ggcggcaata ggcgcagaac ccatacccgc ctcattggag aacacgccgc gcgccacgcc
     5881 gtagcggatg accgtaccga tagcaccgcc cgccactgcc tgcgcgctga acgcatcgga
     5941 gaaaatcagc ttgacggcag gcatcagtgc atcggaatta atcgcgataa tggaaagacc
     6001 gcccaacaca taaaacaccg ccatagcagg cacgatgaaa gaagcggctt tggcgatgcc
     6061 tttaatacca cctaaaacga caacggcagt cagaacggtc aacgtaatgc cggtataggc
     6121 aggttcgata ccgaagctgg tttgcaccgc ctgtgcaacc gagttggact gcaccgagct
     6181 gccgataccg aaggaagcga atgtgccgaa cagcgcaaac gcgacggcca tccatttcca
     6241 gtttttgccc aagccttttt cgatgtaata catcgggccg ccggacattt cgcctttgga
     6301 attgttgacg cggtatttca ccgccaacac gccttcgccg tatttggtgg ccatgccgaa
     6361 aatggcggtc atccacatcc aaaataccgc gcccgggccg ccggttacca ccgcagtcgc
     6421 cacgccggcg atgttacccg tgccgatggt ggcggacagc gcggtcatca acgccgcaaa
     6481 atgggaaata tcgccttcgt ggccttcgcc gcttttatgc ttctttggcg gcataaacgc
     6541 ctgtttcagc gcataaccca acatcgtgaa ctgcaaacct tttaataaaa cagtcagcaa
     6601 aatacccgtg ccgaccagca gcatcagcat caaaggtccc caaacccagc cgctgacggt
     6661 ttcaaaaaag gctttgggat tgtctaaaaa cacttgcatg gctttctcct ttgtctgttt
     6721 tatttttaaa acaccacttt tgtagtgtcc agtaatttca gcacagaata tccaataaga
     6781 caatatgttc ttttgaaaaa tacttttggt tttttcgccg aaaacaggac ggttcaagtt
     6841 gcggaaattg tttgcaattc tttaaaagca gcggcggagg tcacaatgaa atgtccgaat
     6901 ggggatgtgg cgggcggcag aaatcatcaa tgctgccgac tgccatactt ctgaaatcta
     6961 caaaatgatg catcgatcaa acaatatacc gctttaaaaa aaccgatgcc gtctgaaacg
     7021 ctttcggggt ttcagacggc atcaaaaggg tacggtcagc ggatgatgcc gcgcgccgat
     7081 tgtgcgaaaa agtctcggaa tacggcaagc tcggcttggg tttcggcgcg gcggagaatg
     7141 tctgccttgg cttcttcaaa cggaatgccg cgatggtaga gggttttgta cacgtctttg
     7201 acggcggaaa tctgctctgc ggtaaaaccg ttgcggcgca tgccttcgct gttgagcccc
     7261 gccggttcgg cgcggtagcc cgatgccata aagtagggcg gcacgtcttt gtgtacgcct
     7321 gcggcaaacg cggtcatggc gtagtcgccg atgcggcaga attggaaaac cagcgtgtag
     7381 ccgcccaaaa cgacgtagtc gccgatggta acgtgtccgg caagcgaggc gttgttggcg
     7441 aaaatggtgt ggttgccgat gacgcagtcg tgcgcgaggt ggcagtacgc cataatccag
     7501 ttgtcgtcgc cgatacgggt ttcgccgatg ccggttaccg tacctaaatt aaaggtggtg
     7561 aattcgcgga tggtgttgcc gttgccgata atcagcttgg tcggctcgtc gcggtatttt
     7621 ttgtcctgcg ggatttcgcc gaggctggca aattggaaaa tgcggttgtt ttcgccgatg
     7681 ctggtgtggc cgttgatgac ggcgtgcgga ccgatttcgg tattcgcgcc gatttggacg
     7741 ttggggccga taacggtgta cgcgccgact ttgacgccgg agtcgagttc ggctttgggg
     7801 tcgatgacgg cggtcgggtg gatgagggtc atgtttttcc tttcctgtcg tgttgccgcg
     7861 aagatgcgcg acggcaacag gttgtctgaa aactttcaga cgaccttttt ctgaacactc
     7921 aaaccacgcg tttggcacac atgatgatgg cttcgacggc aacttgcccg tccactttgg
     7981 caacggcgtt gaatttgccg atgccgcgcc ggctggtcag cagctcgact tcaaagacga
     8041 gttggtcgcc ggggatgact tggcgtttga aacgggcttc gtctatgccg gcgaagaaga
     8101 agaattcgtt ttctttgcgc ccgccttcgc tcaaaatcgc caacgtgccg cacgcctgcg
     8161 ccatcgcttc gatgatgagt acgccgggca tcacgggcag gtcggggaaa tggccttgga
     8221 actggggttc gtttatggtg acgtttttaa tcgcggtcag ggttttcatc ggctcgaagg
     8281 cggtgatgcg gtcgagctgg agaaacggat agcggtgggg gatgagtttt tggatgtctt
     8341 tggcttcgat ggggagttgt acgtccatgt ctgtcgtatt ccttgaataa agtcggtttg
     8401 gttatttgct gtcttgaccg gcatctgaaa gctgctgctc cagtgttttg agccgtttgt
     8461 tcatttcgct taagcggtgg atgtaaacag cgttgcgcgc ccattcttta tgggtggaca
     8521 tcgggaagat gccggcgagg tgtttgccgc tttcggtaat gctgtgggtg acggacgtgc
     8581 cgccgccgat ggtggttttg tcggcgattt cgatgtgtcc gaccgtaccg acgccgccgc
     8641 cgatgatgca gtagctgcct atggttacgc tacctgagat gccggttttg gcggcgatga
     8701 cggtgtgcga accgattttg cagttgtgtc cgatttggac ttggttgtcg attttggtgc
     8761 cgttgccgac ggtggtgtcg ctcatcgcgc cgcggtcgat gttggtgttc gagccgattt
     8821 ctacgtcgtc gcccagcgtt accgcgccgg tttgcgggat tttgaaccac gaatcgtcgg
     8881 cgaaggcgag tccgaaaccg tccgcgccga tgaccgcgcc gctgtggatt tcgacgcgtc
     8941 tgcccagtgt gcagccgtaa taaacgacgg cgttgggatg caggacgact tcgtcgccca
     9001 gtttgcaatc gtgttggacg acggcgtttg ccaagatgcg gcagccttcg ccgagcacgg
     9061 tgtttgcgcc gatgtagacg ttcgcgccga tttcgcagct ggtgggaacg gtcgcgcccg
     9121 gttcgacgac ggcggtcgga tggatgccgc cgcgcgcttt gacgacgggt gaaaacaggc
     9181 gggcgacttt ggcgaaatag agataggggt cgtcggcgac aatcaggttg cgcccttcaa
     9241 atccgtctgc cgctttggcg gaaacgatga ccgcgcccgc gctgctgtcg tggacttcgg
     9301 ctttgtattt cggattggca aggaagctga tgtgttccgc ctgcgcgtct gcgagcgggc
     9361 gcacggcggt aacggaaatg tcctcgccgc gccattcgcc gccgagccgc gcggtgattt
     9421 gggacagggt gtaggtggcc ggaatcatgg ttttcctgtt cggtatgccg tctgaaaggg
     9481 tcagcgggcg ttcatttctt taatgacgct gtcggtaacg tcgtattggg tgttgacgta
     9541 aatcacgttc tgcaaaatga catcgtaacc ttcctgtttg gcgattttga cgatgacgcg
     9601 gttggcgttt tgctggaggg aggcaaactc ttcgttgcgg cggaggttgt agtcttcttc
     9661 aaactgcgcc tgttttttgc ggaacgctgc gaccagcccg cgccattttt cttcggcttg
     9721 cgcctttttt gcgtttctga gtttgccttc ggcaagctgc ctttccaaat ccagaccttc
     9781 gcgttgcagt ttttgcaatt cgtcctgacg agcggaaaat tcgctgtcca gcgttttttg
     9841 aatcttgcgc gcctgcttgg attcgaggta gatgcgctcg gtgttgataa agccgatttt
     9901 ttggaaggtg tcggcgtgcg cgcctgcggt gcagcacaaa ccgatcagag ccgcggcaaa
     9961 cgcgcgggtc aaacgggtca tggtaaaact ccttcgaatg ttgccgcgaa atgccgtctg
    10021 aagggcttca gacggcattt gcgggattag aacgtcgtgc cgagttggaa ttggaagcgt
    10081 tggatttcgt cttccggttt tttcttcagc gggtaggcgt agctgaattt catcgggcct
    10141 aaaggcgaga gccaggtaac cgcgccgccg gcggaatagc gcaattcgtt ggtaaaggtg
    10201 gatttatggg tattgccggc gccgtaaatg ttttgaaccc tgccgccggt cgcggaactg
    10261 ctgttgtcgt cgtaggtttt gccgtcccac acgctgcctg cgtcggcaaa caggctcagg
    10321 cggacggtgc gcgcgtcttt cgcgccgggc atcgggaaga gcagctcggc ggagacgttg
    10381 gcttttttgt tgccgccgta gctgattttt tcgccgtatt cgtcatagac tttcggaccg
    10441 agcgtgccgc tttcgtatcc gcgcaccgaa cccaggccgc cgccgtagaa gttttcaaag
    10501 aaggggattt ctttggttct gccgtagccg cccgcaatgc cgacttcgcc gccgagcatc
    10561 agcgtgaagg ttttgctcag ggggaagaac caggtttggt tgtgggtggc ggagtagtat
    10621 tgcagtttgc tgccaggcag ggcgatttcg gcgttcacgc ccgtcaggta gccgcgcgtc
    10681 ggccataacg cgctgtcggt tttgttgcgc ccccagccga cggtaccttt gtacagccag
    10741 cctttgaagc tgccgtctgt gccgtcggtt ttgccgtatt tcttgataaa gtcggcatag
    10801 tgtttgggcg ctttgttgta ggtgttgacg gtcaggtgtt ctgccaccaa accgaaattc
    10861 acgcggtcgt attcggtaac aggcacgctc atgcggatgc ctgcgcctgc cgtggtggtt
    10921 ttatattgtt tgatgctggt cgatgctttg cgcgggtcga aggcttttcc gtaaacatcg
    10981 tagcccaggc tgaccccgtc tgccgtgaag tacgggtcag taaacgacag cgagccgtta
    11041 agcgtggttt tgctcctgga ggcgcgcagt gcggccgact tgcccgtacc gaacaggttg
    11101 tcttgggaaa cgcctgcgga catgaccaac ccggtatctt gaacccaacc cgcgctcaaa
    11161 tccagggaac cggtggaacg ttcggtcaga ctcatgttca aatcgacttt gtcgggcgtg
    11221 ccggcaagcg ggacagcatc aaactggaca ttgtcgaagt agcccaaaag ctcgacgcgc
    11281 tctttggaac gttgcagctt ggaggtgtcg taaggtgcgg attccatttg gcgtaattca
    11341 cggcggacga cttcgtcgcg ggttttgttg ttgccggtga tgtgtatttc gttgacgtag
    11401 attttccggc ccggttcgat gtgcaggacg aaatcgacgg ttttggtttc agcgttcggc
    11461 agcggctgta cgctgatttc gctgtatgcg tagcctgccg agcccatgcg gttctgaatc
    11521 tcacccaaaa cggcggtcat ctgctggcgt tcgtaccatt tgccgggctt catggtcagc
    11581 agtttttcca gttcggcttt ggggacttcg ttggtgtcgc cttcgatgga gactttgccc
    11641 caacggaaac gtccgccttc gtggacggtg attttgatgg tctgcttggt tttgtcttcg
    11701 ttggtttgga tgtcggtatc gaggatacgg aaatcgaagt agccgttatt ttggtagaag
    11761 tcggttactt tttccatatc ttgggcaaat ttctgctcgt tgaattggtt gcttcgtgtc
    11821 agccatgtcc aaatgccgcc ttcggtcagg gacatttgcc gcatcagttt gcggtcggaa
    11881 tagacttggt tgccttcaaa ttcgatgtcg gtgattttgg cggatttgcc ctcgtcaatc
    11941 gtgatgtcga tgtcgacgcg gttgcgggcg agtttggtta ctttgggcgt gatttggata
    12001 ttgagtttgc cgcgcccgag gtattcttct ttcaggccgg cgactgcctg attgagtgtc
    12061 gcctgattaa agtattgcga ctgcgccagc ccgaacgatt cgaggttttt cttaatggcg
    12121 tcgttttgca gcatttttgc gccggtgatg ttgagcgagc cgatggtggg gcgttcgata
    12181 acggtcagca ggagctgccc gtccgcagtt tcgacgcgta cgtcgtcaaa gaaaccggtg
    12241 gcgtacaggc ttttgatgat ggcactgccg tgtgtgtcgt tgtaggtgtc gccgactttg
    12301 acgggcaggt agttgaatac ggtactcggc tcggtacgct gcaagccttc gacgcggatg
    12361 tcttggatgg tgaagtcggc aagtgccaaa ggcgatatgc ccaacatcat cagtgcggaa
    12421 gcaatctgtt tcagtttcat tgtcagttcc ttgtggtgcg gaatgcggtt tcagacggca
    12481 ttccgaaacg taaaatctaa ccgagcagcc gggtaacgtc gttgaagaag gcgaccgcca
    12541 tcatcagcat catgagggcg agcccgaagc gcaaaccgat gttttggacg cgttcgccca
    12601 aaggtttgcc gcgtatccat tcggcagtat aaaacacgag gtgcccgccg tccaaaacag
    12661 ggacgggcag taggttcagc acgccgaggc tgatgctgac cagtgctaaa aattccaaat
    12721 aactttgcaa gccgagttcg gcggactgtc cggcaatgtc ggcaatggtc agcggcccgg
    12781 aaatatggct gacggaggcg ttgccgctga ttagtttgcc gaaaaatttg agggttgtcc
    12841 acgagtggga aacggttttt tcccagccca tgccgaatgc gcggacaaca gacggacggt
    12901 agctgcggcg gatttgcgcg tcccacgccc tgtccggctg cggacggagg ccgacgcgcc
    12961 cgatcagggt gtggtcggac tgttcgacag tatcggggcg gatgtcggcg gtatgggttt
    13021 gtccggcgcg ttcgtagttc agggtgattt ttttgccggg gctttggcgg gtcaggtttg
    13081 cccattcttg ccatgaggcg atgggtttgc cgtcggcggc agtcagcctg tcgcccggtt
    13141 tcaggcctgc tttttcggcg gggctgcctt tttccacgcc gccggcaacg gttgtgattt
    13201 taaagggcat cagtccgatg tagccttggt tttttgcgat tttaccggct tccggcgtgc
    13261 ctgcggcatc gatggtgcgg acggtttgcg cgcccgatgc cgtctgaacg ccgacggcga
    13321 ctttgccggc ttcgaggttg aggacgattt cggtttgcgc gctgccccaa tctgcaacgg
    13381 gtgtgccgtt gacggattgt attttgtcgc cgctttggaa gccggcgcgg gcggcaatgg
    13441 tgtcgggttc gactgtgccg acgtaggggc gcagttcggt tacgccgaag gaaaagctca
    13501 gtccgtacag caaaaccgcc agtgcgaggt tggtcagtgg gccggcggcg acgatggcga
    13561 tgcgcttggc ggggtgttgt ttgtcaaaag cgtagggtaa atcggcttct gatacttcgc
    13621 cttcgcgcgt atcgaccatt ttgacgtaac cgcccaacgg aatcggggcg aggcaccatt
    13681 cggtgtcgcc gcgctttcgg gtgaaaaacg gtttgccgaa gccgacggaa aagcgtacga
    13741 ctttgacgcc gcacaatctg gcaacgatgt agtgtccgaa ctcgtgcagg ctgaccaaaa
    13801 tcaggatggc gaagataaaa gctagaaggg tgtgcaaatg gttttccttt gataacggtg
    13861 ttcagatggc atcagcgcag tgtgccgata aatgctcgcg cttgtgcgcg tgtccgggca
    13921 tcttgcgcca agagcccccc tatatcgcct atgccgtctg aaaagtcttg tgcaagacag
    13981 tgggcgacgg ttttggcaat gtcggtaaac ttaatctgtc cgtccaaaaa ggcggcgacg
    14041 gcggcttcgt tggcggcgtt caatacgcag ggcgcggctc cgcctgcgtt catggcttca
    14101 taggcgagcc tcaggcaggg gaagcggtca aagtcgggct tttggaaggt cagcgcggac
    14161 aatgcgtcga aatccaggtc gccgacaccc gaatcgatgc gctcgggcaa acccaaacaa
    14221 taagcgatgg gcgttcgcat atcgggattg cccagttgcg ccagcacgga gccgtcgcgg
    14281 tagcgcacca tgctgtgtat cacggattgc ggatggatga cgacttcgag tttgtcgggc
    14341 ggacagttga acagccaatg cgcttcaatc agctccaaac ctttgttcat catggtggcg
    14401 gaatcgacgg agattttgcg tcccatacgc caattggggt gtttgaccgc ttgggcgggc
    14461 gtaatgcggt cgaacgtgtt taaatcggcg gtcagaaacg ggccgccgga agcggtcagg
    14521 ataatcgaag cgatgccgtg ttcgttcaga cggccggcgt aatcgcgcgg caaaacttgg
    14581 aaaacggcgt tgtgttcgct gtcgacgggc agcactgccg cgccgtttgc acgggcggtt
    14641 tccataaaca acgcgccgga aaccaccagc gtttctttgt ttgccagata aatggttttg
    14701 cctttttgcg ccgctgcgag cgcggaaggc agccccaccg ccccgacgat ggcgcacatg
    14761 acaccgctga cttcgtcggc agaggcaacg tcaaccaatg cctgcgcgcc gtgtaaaacc
    14821 tgagtcgccg tgccgtcgcg tttcaacagg gcttcaagcc gggcggcgtg ttccgcatcg
    14881 gcaacgacgg catattcggg gtggaacgtt tgacattgag ccgccaattt ctcgacctgc
    14941 ttatgccctg ccagcgcgaa tacgcggaat ttttcggggt ggcgggagac aacgtccagc
    15001 gtgctttcgc ctatgctgcc ggtactgcct aatatggtca ggacttgtgg tgtcataatg
    15061 gggataactt tataccggat gccgtctgaa gcgttttcag acggcataga atcaatttaa
    15121 aaccgacatc atcgctgcat agacgctgat aacggcaatc aggctgtcgg tacggtcgaa
    15181 cacgccgccg tgtccgggca gcagcttgct gctgtctttg atgcctgccg cgcgcttgag
    15241 ccagctttcc aaaaggtcgc cgcatacgct gacaacggtc agcaccaaac cgattaacac
    15301 ggtatcgaac cagcctgtat cgaatgccag ccagccggca cttcgtacgg cggtcatgta
    15361 cactgccacg caaaccgcgc cgccgattgc accttcccag cttttgccgg ggctgattgc
    15421 cggcgcgatt ttgtgtttgc cgaacgcctt gccgctgaaa tacgcgcaaa tatcggcaac
    15481 ccacaccaaa cccatcacgg cgagcagcgg cagggcatca tcgggatgcg ggcgcaggga
    15541 tacgagcgcg aaccaaaacg gcatgaccag aagccagccg acggcataaa cctgccaacc
    15601 gccgttgagc ctccatttga atctcaacca taaaggcata acggcgagcc aaaatgccaa
    15661 aacaacatac caaaccaaat taggcagcat ccagccgccc gcataggcaa ccacgccgaa
    15721 aaccaaggtt gcggcgaggt aatggttggt tttaattttg cacaaaccgc ccatacgggc
    15781 atattcccac aaggcaatca gggcaatcag tccgcaaaat gcagcccaca accattgcgg
    15841 cgcgtaaaac agcatgccca gcatcagcgg cagcagccac atggcggtta ttacccgttg
    15901 tttcagcata ttcagttcct ttgctgttcg ataggcagtt gctcggaggt gcgtccgaac
    15961 cgccgttcgc gtttttggaa cgaagcgacg gcatcgtcca aagccttgcc gtcaaaatcg
    16021 ggccacaaaa tatcggtgaa atacagttct gcatatgcca tctgccagag caggaaattg
    16081 ctgatgcgcg tttcgccgcc ggtgcggatg aacaaatccg gttccggtgc atcgcccagc
    16141 atcaagtgtt tcgccagcgt gtcttccgta atctcggata cgccttcggc aatcagtttg
    16201 tttgccgcct gcaaaatatc ccagcggccg ccgtaatcgg cggcaatgct cagggtcagg
    16261 ccggtattgt ttgccgtcaa cgcttccgcc tcttcgatgc cttgcagaat ctgccggttg
    16321 aagcgttcgc ggctgcccaa tatcttcagg cgcatattgt tttcgtgcag gcggcgtacc
    16381 tgtttttgca aagcctgtaa aaacagcccc atcaggaacg aaacttcgtc ttcggggcgg
    16441 cgccagtttt cggttgaaaa ggcaaacacg gtcagatatt gcacacccag tttggcgcaa
    16501 tgcttcacca tattttccaa tgcgtccaaa ccgcgtttgt gtcccattat gcgcgggagg
    16561 aaacgttttt tcgcccaacg gccgttgccg tccataatca cggcgatatg cttgggaatg
    16621 gcggtgtgtt ccaaaacggc ctgcgtgctg cttttcatgt ctgcctttcg cggttcggca
    16681 ttcaaatgcc gtctgaacgc cgaaccgtgc aggttaaatt gccatcaaat cttcttcttt
    16741 ggcagtcagg agtttgtcgg cttcggtaat gtatttgtcg gtcagttttt gaaccgcttc
    16801 ttcgccgcga cgtgcctcgt cttcggaaat ttctttgtct ttgaggagtt ttttgatgtg
    16861 gtcgttggca tcgcggcgca cgttgcggat agagacgcgg ccttcttccg cttcgccgcg
    16921 tacgacttta atcaggtctt tgcggcgttc ctcggtcagc atgggcatcg gcacgcggat
    16981 caggtcgccg acagctgccg ggttcagtcc caagtttgaa tcgcggatgg ctttctcgac
    17041 tttggccgcc atattgccct caaacggttt cacgccgatg gtgcgcgcgt ccagaagcgt
    17101 tacgttggca acttggctga cggggaccat gctgccccag tattcgactt ccacttggtc
    17161 gagcaggccg gtatgcgcgc ggccggtacg cactttcgcc agattttctt tcagtacttc
    17221 gaccgaacgc tgcatcttgc cttcggctgt tttttgaata tcgttgatca tattgttctt
    17281 tcggtgggat aaggtgggcg ggagaccgtc tgaacgcgtt tcaagccgtt cagacggcat
    17341 aaagaccgtt aaccgcgaat agtaccgtta ttcgggcata acgacaaggt aggcggattg
    17401 gggatgccgt ctgaagcgac aggcgtttca gacggcatcg tgtccgaccg tcagccgtgt
    17461 tcccgtgttt caagcaggct ttggcgcagg tgttggcgtt cgtgggcatc cagccatttg
    17521 cggcgggtgc gttgcagcag gatgacgagg gcggaaattt cctgacgcat attggtgctg
    17581 agccagagga agccctgcca ttggtagtgg aggtgttcgg cgagggcttc cagttcgggg
    17641 ttgatggcgg tgtcgatgcg gatgcggcgg gcgtgtctgc cgttgataag ggcgacggtt
    17701 tgttgcaggt cggtttggag cagtgtgaag tggcggtcaa gcagccggat ttcgctgccg
    17761 ttgagtttgg gagattgcag cttggcggcg gtggtcagga gcagctcggt ggtgttgacg
    17821 attttacggt gggcgtgctg catggcttcc atcatggcgg ggctgatgcg gctttcgccc
    17881 gatgtggcgg cgagatggct gcggcttttg accatgcgtg cgttgatttg gcgcattttc
    17941 gccatgttct cctcgaggcg ttcgcgggtc atgcgcctgc cgttgctgat ttcggcaatc
    18001 attttgctgc agtcggccag gttgtcggca agcatgaaac gccacatcag tgtggatttc
    18061 agcggcagca gtttggcggc ggcgatggcg atggccgcgc cgatgaggac gttcatggcg
    18121 cgcatgagtc cgctgtcgag ccattcgctg ccgttgtcgc cgatgagcat acacatcgtc
    18181 agccctgcca gcatagggac gtagccgttt ttgccgaccg ccgcccagcc ggccagtgcg
    18241 cttgccgtgc cgacggtgag gtagaagagg aggttgccgt ggaaataatg ctggttcagc
    18301 cataaaacgc ccaaacccgc gcccagcccg atgaccgtgc cgagcatacg ttccaccgcc
    18361 ttggagtaaa tcgccccttg aaactggagc atgccgagga cgacgaagac ggtcatccct
    18421 atccactcgc cgtgttggag gtggagcagc cgggcggagg cggtggcgaa caggacggcc
    18481 ccgccgagcc ggacggcgtg gatgaggcgg cggtagcggt agcgttcgta ggagttgagc
    18541 cagcggctga cgaggcggtt gcgttgcgag gtgttcatat cggttgtgcc gtctgaagcg
    18601 gaaatgtgaa aaagcacagg cttcccgagg aagggagggt ctgtgcttgg tattggtgcc
    18661 ggagaaggga atcgaacccc cgaccttcgc gttacgaatg cgctgctcta ccgactgagc
    18721 tacaccggcg ttttttcgtc atgatatata tgaacggttg tttgtgcaac ttttcgggcg
    18781 ggcggcaagg cagtgcg
//
LOCUS       DAWGRZ010000021        18117 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000021 DAWGRZ010000000
VERSION     DAWGRZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18117
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.021"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            155..991
                     /gene="cysT"
                     /locus_tag="WHT44_000510"
     CDS             155..991
                     /gene="cysT"
                     /locus_tag="WHT44_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ2532736.1"
                     /translation="MLALKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGASRWTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            1243..2103
                     /gene="cysW"
                     /locus_tag="WHT44_000511"
     CDS             1243..2103
                     /gene="cysW"
                     /locus_tag="WHT44_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ2532737.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIVLATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAI"
     gene            2100..3173
                     /locus_tag="WHT44_000512"
     CDS             2100..3173
                     /locus_tag="WHT44_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ2532738.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGNILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKIHAVGALTHILVKHDKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(3230..4756)
                     /gene="ilvA"
                     /locus_tag="WHT44_000513"
     CDS             complement(3230..4756)
                     /gene="ilvA"
                     /locus_tag="WHT44_000513"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ2532739.1"
                     /translation="MNTPLPYSDYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSHGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTNNEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            4904..6073
                     /locus_tag="WHT44_000514"
     CDS             4904..6073
                     /locus_tag="WHT44_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ2532740.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNRPTVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLALLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVKKRSRWQRLWACLTGQ"
     gene            complement(6198..6770)
                     /locus_tag="WHT44_000515"
     CDS             complement(6198..6770)
                     /locus_tag="WHT44_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ2532741.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(6837..7820)
                     /locus_tag="WHT44_000516"
     CDS             complement(6837..7820)
                     /locus_tag="WHT44_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ2532742.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            complement(7987..8062)
                     /locus_tag="WHT44_000517"
     tRNA            complement(7987..8062)
                     /locus_tag="WHT44_000517"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8027..8029),aa:Gln,seq:ttg)
     gene            complement(8071..8146)
                     /locus_tag="WHT44_000518"
     tRNA            complement(8071..8146)
                     /locus_tag="WHT44_000518"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8111..8113),aa:Gln,seq:ttg)
     gene            complement(8159..8234)
                     /locus_tag="WHT44_000519"
     tRNA            complement(8159..8234)
                     /locus_tag="WHT44_000519"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8199..8201),aa:Gln,seq:ttg)
     gene            complement(8242..9087)
                     /gene="ispE"
                     /locus_tag="WHT44_000520"
     CDS             complement(8242..9087)
                     /gene="ispE"
                     /locus_tag="WHT44_000520"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ2532743.1"
                     /translation="MNIADGRQAFSAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVDGMPQEVDLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFARGIGDRLD
                     EMDIPKQWYVIVKPPVHVSTAKIFTHESLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PLLSV"
     gene            complement(9097..9678)
                     /locus_tag="WHT44_000521"
     CDS             complement(9097..9678)
                     /locus_tag="WHT44_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ2532744.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            complement(9818..11662)
                     /locus_tag="WHT44_000522"
     CDS             complement(9818..11662)
                     /locus_tag="WHT44_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532745.1"
                     /translation="MLPNRFKMLTVLTATLIAGQVSAAGGGAGDMKQPKEVGKVFRKQ
                     QRYSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPE
                     VAERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLE
                     EVLAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSV
                     QGREKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQ
                     EMEIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAY
                     GRGTEEQRSRAALTAAMMYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGG
                     RAALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPAGS
                     NTELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLTDSKRLD
                     EGFALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGE
                     VLWALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            complement(11827..12663)
                     /locus_tag="WHT44_000523"
     CDS             complement(11827..12663)
                     /locus_tag="WHT44_000523"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ2532746.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLAAEGIAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFHIVSPDIACFGKKDYQQLAVIKGFVED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLVQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRADTLEVARAGDKKLVVLAAACLGTTRLIDNLEIK
                     LP"
     gene            complement(12786..13577)
                     /gene="panB"
                     /locus_tag="WHT44_000524"
     CDS             complement(12786..13577)
                     /gene="panB"
                     /locus_tag="WHT44_000524"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ2532747.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRKSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVLAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMQGHDSVQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            complement(13719..13794)
                     /locus_tag="WHT44_000525"
     tRNA            complement(13719..13794)
                     /locus_tag="WHT44_000525"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13759..13761),aa:Thr,seq:tgt)
     gene            complement(13829..13904)
                     /locus_tag="WHT44_000526"
     tRNA            complement(13829..13904)
                     /locus_tag="WHT44_000526"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13869..13871),aa:Thr,seq:tgt)
     gene            14070..14861
                     /locus_tag="WHT44_000527"
     CDS             14070..14861
                     /locus_tag="WHT44_000527"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ2532748.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            14916..15557
                     /locus_tag="WHT44_000528"
     CDS             14916..15557
                     /locus_tag="WHT44_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ2532749.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSGGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARTPDLLLLIGAAVCHYTDRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(15635..16558)
                     /locus_tag="WHT44_000529"
     CDS             complement(15635..16558)
                     /locus_tag="WHT44_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ2532750.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHSLFEPGKVMFYYRETSRESEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            16722..17591
                     /locus_tag="WHT44_000530"
     CDS             16722..17591
                     /locus_tag="WHT44_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ2532751.1"
                     /translation="MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            17634..18068
                     /locus_tag="WHT44_000531"
     CDS             17634..18068
                     /locus_tag="WHT44_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532752.1"
                     /translation="MKKQISSAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGEWIPNAVGGIMGGVGGHFSYMASAIASGSYNRNEHWTAIGGGALIG
                     AMNPINGGRALINGMRGVAVGTVAGGINGYAGSTGKNTDIRR"
BASE COUNT         4445 a         5180 c         4281 g         4211 t
ORIGIN      
        1 ttaatccact atattttaaa acatcgcgcc cgcttgagaa actgccaacc gctttataac
       61 aaattcgtct ttgcaccaaa ctttccattc tttccgtttt tcggacggca tcgttaaagt
      121 agtccttcct tttccttatt ttcagcattg ttttatgtta gcgctcaaaa cgcccggcgt
      181 actgcccggc ttcaaactca gccttggtct gaccgtattg tgcctgtcgc tgcttgtggt
      241 cttgccgttt gcgatgatgg cggcgaaggc ggcggaaatc ggctggggcg gcttttggaa
      301 cacgattgcc gagccgaacg tgttggcggc ggtatggctg agcttgcgga tgtcgtttta
      361 tgcgatgctg accaatgtcg tgttcggcac gctggtggcg tgggtattgg tgcgttatga
      421 attcccgggc aagggtctgg cgaacgcgct ggtcgatttg ccgtttgcgc tgccgacggc
      481 ggttacgggt atcgcgttgg caaccctgta tgcgcccaac ggttggatag gccgtttttt
      541 cgagcctttg ggcatcaaaa tcgcgtttac acccgtcggc atttggattg cgctggtcgt
      601 cgtcagcctg ccctttatcg tccgcgccgt gcagccggta ttggaagaat tgtcgggcga
      661 atatgaggaa gcggcggcaa ctttgggcgc aagccgttgg actacgtttc gccgtgtcct
      721 cttgcctgaa atcacaccgg cactcttgac cggcgcggga atgatgtttg cgcgggcaac
      781 gggggaatac ggttcggtga tttttatcgc gggcaacatt ccgatggttt ctgaaatcct
      841 gccgctgatt attacgggca agctggaaca gttcgacgtg cagggcgcgt cggcggtggc
      901 gttgtttatg ctgctggttt cgtttgtgat tctgtttgcg ctgaacgtga tgcagtgggc
      961 gttgggcagg cgttcgggcg cgaagggttg aggtcgtctg aaatacctgt taccgtcatt
     1021 cccgcgcagg cgggaatcca ttggtgaatt tcggctgcct tatttatttt ctgttttcct
     1081 gttgccctgc ggtggattcc cgcctgcgcg ggaatgacgg tagctagacg tttttattcc
     1141 cttaatcaat aaaaggttgt ctgaaaacga atccgcccca caaaaaacgg tttttcagac
     1201 ggcatccaaa cattttaaaa ccaaccagag aacaccaccg ccatgaaacc ctattccgcc
     1261 aatcccaacc tgaccgaacc gcgccggctg cgcgtgttgc tgattgccgc cgcgctgggc
     1321 tttctgctgc tgatgctggt cgtgccgctc gtcgccgtgt tttacgaagc cttaaaaggc
     1381 ggttgggatt tgtacctgaa atccttaaac gatcccgaag cgtggtctgc catcaaattg
     1441 acgctgatta ccgcgctgat tgtcgttccc gtcaatgccg tattgggtgt ggcgatggcg
     1501 tggctgctga cccgttttga ttttcgcggc aagcagttgc tgaccaccct gctcgatttg
     1561 ccgttttccg tatcgcccgt ggtggccggt ttgatgttcg tcttattgtt cggcgcgcat
     1621 acggcattgg gtggctggct cgaagcgcaa ggcatacaga ttatcttcgc catccccggt
     1681 attgttttgg cgacgctgtt cgttaccttc ccctttgtcg cacgcgaaat catcccgctg
     1741 atgcaggcac agggcgacag cgaagaacag gcggcattga tactcggcgc aagcggctgg
     1801 cagatgtttt ggcgcgttac cctgcccaac atcaaatggg cgttactcta cggcatcatc
     1861 ctcaccaacg cccgcgcgat gggcgagttc ggcgcggtca gcgtggtatc gggacacata
     1921 cgcggcgaaa ccaacaccgt cccgcttttg gtcgaaatct tctacaacga atacaacttc
     1981 accggcgcat tcgccctctc cggcgtattg gcacttttgg cactggcgac gctggcggtg
     2041 cagaacatca ttaccaaatt acaagacaaa aaactcgccg ccgccgaaag gaatgcaata
     2101 tgagtatcac catccaaaac ttaaacaaac acttcggcaa ttttcacgcg ctgaaaaaca
     2161 tcaacctcaa cgtccccacc ggcaaactcg tttccctgct cggcccgtcc ggctgcggca
     2221 aaaccacact tttacgcatt atcgccggac tggaaaacgc cgacggcggc aatatcctgt
     2281 ttgacgggca agacgtaacc gccaaacatg tgcgcgagcg caaagtcggc ttcgtgttcc
     2341 aacactacgc ccttttccgc catatgaacg tgtttgacaa cgtcgctttc ggtttgaccg
     2401 tattgcccaa gtccgaacgc ccgtccaaag gacaaatccg cgccaaagtc gaagaattac
     2461 tcaagctcgt gcagctctct catttggcaa aatcctatcc gcaccaactc tccggcgggc
     2521 aacgccagcg catcgccctc gcccgcgcgc ttgcggtcga acccaaactc ttgcttttgg
     2581 acgaaccctt cggcgcgttg gatgccaaag tacgcaaaga attacgcacc tggctgcgcg
     2641 acatccatca caacctgggt gtaaccagca ttctggttac gcacgaccaa gaagaagccc
     2701 tcgaagtttc cgacgaaatc gtcgtgatga accacggcaa aatcgaacaa accggcagcg
     2761 ccgaagctat ttaccgcaaa cccgaaaatg ccttcgttac cgagttcctc ggcgaaaccg
     2821 acgcttttga aggacgcatc gaaaaaggct tctggcatta caacggcttc gcgtggaaat
     2881 tggacgcgca atacaaatgg caggaacaaa ccgccaccgg ctatatccgc ccgcacgaat
     2941 ggcagatcgc cgccgaacac gaaacaccga tgatttgtgc cgaaatcgaa aaaatccacg
     3001 ccgtcggcgc attgacgcat attctggtaa aacacgacaa acaggacgta catatcacgc
     3061 tggcaggcag cgatgccgcg cgttacccaa tcgccgaagg caaagaattg aagctgattc
     3121 cgaaacaggt ttatgtcttc tctcaaaacg aactgattga atattcgatt taaccatgaa
     3181 agcgcaatgc cgtctgaaag gctttcagac ggcattgtgc tttcaagcgt caggcaagaa
     3241 acagcttgta cgcggcattt tgcgtttcct cgtgatagct gtatcccaga ctttccaaga
     3301 aaccgtcaaa tgcggcggca tcgtgcggcg gcacatcgat accgaccaaa atccgcccgt
     3361 aatccgcacc gtggttgcgg taatggaaaa gcgtaatatt ccaccctccc tgcatatggt
     3421 tcaaaaagcg tgccaatgcc cccggacgct ccggaaactc aaaactgacc aaacgctcgt
     3481 tttctacttt gtccgtccgc cctccgacca tatagcggat atggattttg gcaatctcat
     3541 tgttggtcaa atcgacattg ggcaatcccg cctcatccaa ccggctgccg ataaccgcca
     3601 aatcctgcgg gcctgccgct tgaagtccga caaagatatg cgctttttca tcgtctccgt
     3661 agcggtagtt gaactcggta atattcctat ttcccaatat attgacaaac ttaaggaagc
     3721 tgccgcgttc ttcagggatg gtaacggcaa aaataccttc gttgccctcg cccaattcgc
     3781 tccgttccga aacgtggcgc aaacggtgaa aattcatatt cgcaccgctg gtaacggcaa
     3841 tcagggtttg gttttccgcg ccttctcggg cgatataggc tttcagaccc gccaacgcca
     3901 acgcgcccgc cggctcggta atgctgcgcg tgtcatcgaa aatatccttg accgcgccgc
     3961 aaaccgcatc ggtatcgact gtaatgattt catccaaaag ttctttgcag aggcggaagg
     4021 tttcgtttcc gacgactttg accgcagtgc cgtctgaaaa cagcccgaca tctttcaaat
     4081 ggacgatttc acccgcttcg accgactgct tcatacagca ggaatcgttg gtctgaacgc
     4141 cgataacttt gatttcggga cggacctgct tgataaatgc cgccacgccc gccgccaaac
     4201 cgccaccgcc tatcggtacg aatacggcgc ggattggatc gggatgctgg ctgacaattt
     4261 ccatccccac cgtcccctgt cccgcaatca catcaggatc atcaaacggc gcgatatagg
     4321 ttaacccttc tttttccgcc aactccatcg cataatcgta ggcatcgttg tatgaaacgc
     4381 cccgcaaaac cacctcgccg ccatggcttt taaccgcatc cactttgatt ttcggcgtag
     4441 tctccggcat aacgataacg gcacggcagc ccaaacgctg tgcggacaat gccacgcctt
     4501 gagcatgatt gcccgcgctt gccgcaatca cgccgcaagc gagcgcatct ttcggcaact
     4561 tggacatttt gttgtacgcg ccgcgtattt tgaacgaaaa aaccggctgc aaatcttcgc
     4621 gtttcaaaag gatgttgttt ttcaaacgta cagaaaggct gcgtgccggt tccaaaggcg
     4681 tttcgaccgc cacatcatag acagatgccg tcaggatgcg gatgaggtaa tcggaataag
     4741 gaaggggcgt gttcataatt caatatggga taatcggttt attaaaatcg caaaacccaa
     4801 aaccatacgc ccaagacggc gcgaaatcaa gaaaaatccg cccgatcaga caccctaagc
     4861 gtataatcgg cagactgaaa cacgcacaca attagaatat ttcatgacag cacataaaat
     4921 cctgcccgtc ctgctttcca tcatcttagg cgtttctcac gcaacggctg catcgcccgc
     4981 gcccaacaga ccgacggtac acgccgcccc cacgttccaa acacccgaaa ccctcacagc
     5041 ggcacacatc gttatcgacc ttcaaagcaa acagatttta tccgccaaaa acatcaatac
     5101 ccctgttgaa ccggcggcac taacccaact gatgaccgca tatctagttt tcaaaaacat
     5161 gaaatcgggc aatatccaat ctgaagaaaa cttaaaaata cccgaatccg catgggcttc
     5221 agaaggaagc agaatgtttg tacgtcccgg cgatacggtc agcaccgaca aactcttaaa
     5281 aggcatgatt gcactatccg caaacgatgc cgccctaacc cttgccggcc ggctgggcaa
     5341 cggctcgatt gaaaattttg tgcaacaaat gaacaaagaa gcccgacgct tgggcatgaa
     5401 gaacactgta ttcaaaaacc cgacaggctt gagtagagaa ggacaggttt ccaccgccaa
     5461 agacctcgcc ctgctgtctg aagcattgat gcgcgacttt ccggaatatt acccgctgtt
     5521 ttccatcaaa tctttcaaat tcaaaaatat agaacaaaac aaccgcaata tccttttata
     5581 tagggacaac aatgtaaacg gtctgaaagc cggacacaca gaaagcggcg gctacaacct
     5641 tgccgtgtca tactccggca acggcaggca catccttgtc atcacattgg gttcggaatc
     5701 ggcggaaaca cgcgcatcag acaacagcaa gctgctgaac tgggcattgc aggccttcga
     5761 tacgcccaaa atatatccga aaggcaaaac cgttgcccaa atccaaattt ccggaggcag
     5821 caaaaaaacc gtccgcgcag gcttcctcaa agaagcctac atcactctgc cacataagga
     5881 agcgaaaatg gcagaacaaa ttctagaaac catacagccg attcccgccc cagtaaaaaa
     5941 agggcaaatt ttaggaaaaa tcaaaatcag acaaaacgga tacaccattg ccgaaaaaga
     6001 aatcgtcgca ctggaaaatg taaaaaaaag aagccggtgg caaaggcttt gggcgtgtct
     6061 gacagggcag taatctgccg tttcaaatat cccgtttttc caacaaataa agaaatgccg
     6121 tctgaaacac ggttcagacg gcataaaaca acagggcggt acgtattgca tacgcgccgc
     6181 cctgctgctg aaatcaatta gcgtttctta ccggtaacgg tagcaacagc cagattttcg
     6241 ttacgtttca gggaaacgct ttctacacct tcaggcagtt tgatgtctga caagtgcaga
     6301 atgtcgccgg caaccacttc agcacaatcc aaatccaaga aagcagggat gttggcaggc
     6361 aaagcaacta cttcaacaga agtgtttaac agagatacgc ggccgccttg cagtttgacc
     6421 gcttgggaat tttcagcgtt aacgatgtgc aggggaacac ggatgcgtac aagttgatcg
     6481 gctttcacag cttggaagtc gatgtgttga acttcgcggc ggaacgggtg catttggaaa
     6541 tcacggacga taacgtcttt ggtttcaccg ttcagagaca acttaatcaa cgcagtatgg
     6601 aaagattctt tttccaatgc gtagaatacg gttttgtgat ccacagcgat tgcaacaggc
     6661 tcttgacctt caccgtacag aatgccgggg atttggcctt cgcgacgcag gcggcggctc
     6721 gcaccagtgc cttgtgcttc acgaacagag gcttgaattt cataagtcat gttaaatact
     6781 ccaagttagg taaaatcgcc gtcatcggcc gcgaccagct taagacggct tcgggcttat
     6841 ggcagcaaca tgctgcctgt catcacttct tcattgaaaa gatatgagac ggattcttca
     6901 ttgctaatgc ggcggacggt ttcggccaac agaccggcaa tcgttacctg acggatacgg
     6961 tcgcagtttt tagccgcttc agacaaagga atggtatcgg ttacgaccac ctggtcgatt
     7021 tcggatgagg cgatacggct gaccgcctct ccggagaata cggcgtggct ggcataggct
     7081 agaacacgtt cagccccccg ctctttcagg gcgacggcgg ctttgcacag cgtatttgca
     7141 gtgtcaatca tatcgtccac aatcagacag gttctacctt gaatatcgcc gatgatgttc
     7201 atgacttccg ccacattggc tttcgggcgg cgtttgtcga tgattgccaa gtcggcattc
     7261 agggattttg ccacggcgcg ggcgcggacg acaccgccga tgtccgggct gacgacggtc
     7321 agattttcaa tccgctgttg tttgatgtcg ttcaacagaa tcggggtggc ataaatattg
     7381 tccaccggaa tatcgaagaa accttgaatc tggtcggcat gcaaatcgac agtcaaaaca
     7441 cggtcgatcc ctgccgaata cagcatattt gccaccagtt tggcagaaat cggaacgcgg
     7501 acggaacgcg gacggcggtc ttggcgcgca tagccgaaat acggaatggc tgtggtaata
     7561 cgacctgccg aagcacgctt cagtgcatcc gccatcgtca ggatttccat caggttgtca
     7621 ttggtcggcg cacaggtcgg ctgaaggatg aaaacatcgc gcccgcgtac gttttccaac
     7681 agttcgacgg caacttcgcc gtctgaaaac ttggatacgg aagcattgcc caaagaaatg
     7741 tccaaatgcc tgacaacacg ttgtgccaat tcgggattgg cattgcctgt aaataccatc
     7801 aaactgtcgt acgcagccat attctcacct gattttatgt ttaacttccg ctcagaaaac
     7861 acaatgcttc cctctgcttg taccaagccc gacattatac ctgcaacatc cgacacaaac
     7921 aaaggcattt caatattttt atttctatga aataaaagcg tgtaagcagg cttacacgct
     7981 tttatttggc tggggaggaa ggattcgaac cttcgcatgc tggaatcaaa atccagtgtc
     8041 ttaaccgctt gacgactccc caaaaagggc tggctgggga ggaaggattc gaaccttcgc
     8101 atgctggaat caaaatccag tgtcttaacc gcttgacgac tccccaactc gtttgacttg
     8161 gctggggagg aaggattcga accttcgcat gctggaatca aaatccagtg tcttaaccgc
     8221 ttgacgactc cccaacaatg tttatacgga caacaaagga tgttttgaaa gaccctctgc
     8281 caaatatgcc tcgtacaaat ctgaaacttg tcggtatata ttgtatgcgc tattcctatc
     8341 ttgacacgcc gtgaatacac acgcaccgga acctgtcatt aaggcaaatc catatcggga
     8401 caactcggaa taggctttcc aaacttcagg gtattcttta aatacaactg cctgcatatc
     8461 atttctaaac ggttgcagat tttggaaagt cggcattatg cttgaggcgg aatttcgtgt
     8521 caagctttcg tgtgtgaaaa tttttgcagt ggaaacgtgg acgggcggtt tgacgatgac
     8581 ataccactgt ttcggaatat ccatttcgtc cagcctgtcg cctatacccc gcgcaaacgc
     8641 atttttgccg aaaataaaaa acggtacgtc cgcccccaga gccgcgcccg aatcaatgag
     8701 ctgccgctgc gtcagaccgc actgccacca acggttcaac accagcaaaa cggttgccgc
     8761 atccgagctt ccgccgccca aacccgcccc tgtcgggatt tttttgtcca gccatatttc
     8821 cacgccggcg gggttgcgcg catatttttg cagcaacgat gcggcacggt agctcaaatc
     8881 tacttcctgc ggcatgccat caaccggatt gtgcaggatg attttgccgt cgtccctcgg
     8941 tttcaaatat acggtatcct gcaaatctat caggcagaat atgctttcga tattgtgata
     9001 accgtcttcc cgcctgccgg taatcctcaa atcgagattc agttttgcag gtgcggaaaa
     9061 cgcctgccgt ccgtccgcaa tattcatctg tccgccttat ctcgtgcgcg ccgcacagcg
     9121 ttccggggtt tcggtttcag acggcatacc gatttcggtg aaaaccagcc tgatgttcaa
     9181 atttccgtta ttcagttgca acgttcggac ttgcccccca ctgtcggcgg ttctgccgac
     9241 agtccaaccg tattgttcca atatgccgtc cggcaggatg cggtaaggcg cgcccgccac
     9301 acgcctgcca tctgcccaga tatgcagata ttggattggc agtttgaaac cgaccagctg
     9361 cctgctcaat tcttccgcac tttccgcctg atagacattt cctttgccgt ccactgccaa
     9421 tgcgccgtcc ctgtcttgac acaactgccc gagcgtactg cccaaagggg tattgatatt
     9481 gatggtttcc acgggcggtt ggtatgtcca atcgaaattt gcatacgaac ctttcccttc
     9541 cgctttcact gccaaccgcc cttctgctgc aaaactgctg atgtgttcgg acggctgcca
     9601 caggttttcg ttattttgag gtaattgcgc gcaagcggtc aaaagcagga tgaccgatgc
     9661 ggatacggtg tgtttcatca gaatttcctt aacggatgcc atcctgccgg ccgatgggtt
     9721 cggcgtgccg tgcaaaacgc gtgcgaactg cgtgccgttt gcccgaatgc cccggcttcc
     9781 cggcaaacct tatgccgtct gaaaggatgg acctgcatta tttccgaggt tttcgggaag
     9841 gttggggcaa tgcgatgccg tgacgtttga gcgtttcccg ccatattttc ttgtctcccg
     9901 taaggtgtgc cgcctgcgtc catacgtcaa ccgcctgatc gcgttcgccc aatgcccaca
     9961 acacttcgcc caaatgggcg gcaacttcgg gctcggggtc gttttcaaac gaataccgca
    10021 gatacggcag cgcgctttcc gcgtcgcctt tcaggtaata cgcccagcct atgctgtcgt
    10081 tgacagcggt atcgtccggg ttgatttggt atgccgtctg aagcagggcg aaaccttcgt
    10141 ccaaacgttt ggaatcggtc agcaggctgt agcccagatt attcataatc tgagcgttat
    10201 cgggtgcaag cctgaacgcc ctttcaagat ctgaaatcat ttttttccgc ttgccaagcc
    10261 gatcgtaaac aactgaccgc tgtaccaatg cctctgcctg taactctgta ttactgccgg
    10321 caggcggttt ttcgataatc ttgtccaacc ccctcaaagc ctcccgttta tcgggcagct
    10381 tcgacagggc gagcatctgt attttggaca aattgtctgc cgtaaaatac cgcccctgct
    10441 gttcgggaag tttccgcacc ctgccgatct gccgcaaagc cgccctgccg ccgtccaact
    10501 cgacagccgc cgcagccgcc agcacacctt tgtcgaacag gtattccggc gcggatactt
    10561 ttttcagcca ctgcctgact ttggcgtaat ccctgcggtc ggcatacatc atcgccgccg
    10621 ttagcgccgc cctgctccgc tgttcctccg tccccctgcc gtatgccttt tcggcgtagc
    10681 cgtcgataac ggaagcacct tcttttcggt ttgccgccaa tatcgctgcc tgaatataca
    10741 ggtctgcatt cggattgcgt tccaacagca cgttcaaacg cgcataggca tcatccagcc
    10801 tgtgcaggga aaccagattc ataatttcca tttcctgcca gacggccgaa aggttttggg
    10861 tgtctgtctg ctcgaaaaag ccgtcgagta tttcgggata tttgcgtgca gtcagacgca
    10921 acgtcattaa agtggggggc aatatttccg tatcgagctt cgccaaacgc tgcaaagctc
    10981 cgattgcctt ttccttttcg cgtccctgta cgctgaacac cacatcggca accgccgctt
    11041 cgggcagatg ttcatatttc aacgccgcgc ggcgaaccgc tttcgatgct ttttgcgcca
    11101 acccgtcctg ttgcacggcg gcttgtgcca acaataaaaa caccctgcgg ttctgtcctt
    11161 cgtccgcctg agccagcact tcttccagtc cgtccagatg ctgatttcct ctttccctca
    11221 gcacgttccg cagccacccc gcccgttttt gcgccttacc cggtataggc tcaatctgcc
    11281 gccatttctg ataaatcatt tccgcctgtt caaacgcgtt cagcgacacg gccatttcca
    11341 aggcgcgttc ggcgacttcg ggggattttg tgcgttccaa catcagcata taggttgcca
    11401 gagccgttcc cgcctgcccc ttttgcaagg cggtttcccc tcccagcaac gtaaatatct
    11461 gattaacccg ctcgcccact gccgcaagcc gtgcgcgttc gtttttgatt tcttcctcgc
    11521 tgtaacgctg ctgctttctg aaaacctttc cgacttcctt cggctgtttc atatcccccg
    11581 caccgcctcc ggcggcagat acctgtccgg caatcaaggt tgccgtcaac acagttaaca
    11641 ttttgaaacg gttaggtaac ataatcatcc tttatccgac agccgtccga accttcagac
    11701 ggcatataat caggcgtaac tttatcacaa gcagaatcgt ttgtagattt gcgaaacccc
    11761 gttcaaaaaa ataaatgccg tctgaaacgc aaatccgctt cagacggcat tcccgacact
    11821 tgcggtttaa gggagtttta tttccaaatt gtcaatcagg cgcgtcgtcc ccagacaggc
    11881 ggcggccaag accaccagtt tcttatctcc cgcccgcgcc acttcgagcg tatcggcgcg
    11941 gcggatttcg acataatcga ccacccagcc gtattctgtc agggattgga cggcacgttt
    12001 ttccaaacct gcataatcca aactgccctg caccaaggat tcggcaacag cctttaattc
    12061 gcggtacagg cgcggtgctt cgtcgcgttc cgccgcactc aaatactggt tgcggctcga
    12121 cagtgccaac ccgtcttccg cgcgccctgt atcaacaggc actatttcaa catcaaaatt
    12181 caaatcttcg acaaaacctt taatcacggc aagctgctgg taatccttct taccaaaaca
    12241 ggcaatgtcc ggggaaacga tgtggaacaa tttagaaaca accgttgcca caccgcgaaa
    12301 atgccccggg cggaatttgc cgcacaactc attttgcaga ttgggcggtt cgacgttgta
    12361 acgctgttcc acgttcggat agagttcttt ctcatcgggc gcgaaaacaa cggcaatgcc
    12421 ttcggcggca agtttgtccg catcctgttg caaagtgcgc ggatatttgt cgaaatcctc
    12481 gccctgaccg aattgcaggc gattgacgaa aatgctgacc acgacactgt ccgcgcgttt
    12541 tttcgcctca cgcacaagcg caagatgtcc ttcatgcaga ttgcccatgg tcggcacaaa
    12601 tgccaccttt cccgcatttt tacgccacgc gcgcagttct cgaatggtat gtatgatttg
    12661 cataacggta atccttatcc ttcgggcttc ctgcccgaca aaacgcgccg attatacgcg
    12721 ccgcgcacgg caaaacaaaa tgccgtctga aacggctttc agacggcatg ctgcatgtaa
    12781 accgctcaat ctgcaaaaat atgttccgca gcagggaagg ttttggcttt gacttcggca
    12841 acatacgccc gaaccgccgc ttgaacacta tcatgcccct gcataaagtt tttgacgaat
    12901 ttcgccgtct tacccgggaa aatgccgagc atatcgtgca tcaccaaaac ctgcccgtcg
    12961 caatccgcac ccgcgccgat gccgatggtc ggacaggaaa cagtttcagt tacctttttt
    13021 gccagttccg ccagtacgca ctccatcagc acgaccgccg cccccgcatc gtcatgcgcc
    13081 ttggcatcgt taagcaacgc ctgcgccttg ccgccgcgcc cctgaacttt atatccgccg
    13141 aaggcaaaca cggattgcgg ggtcagaccg atgtgcgcac aaaccggaat accgcgcatt
    13201 tgcaaaaatt cagtcgtttc cgccatccac acgccgcctt cgagtttaac catatgcgcg
    13261 ccggcagcca tcagttcggc ggcggcggca aacgcctgct ccttactctg ctgatatgca
    13321 ccaaacggca aatcgctgac aatcatcgca ttttttgcac cgcgtgctac acattcggtg
    13381 tgataacaca tatcgcgcag gctgacgggc agcgtcgatt tccgcccctg aaccgccatc
    13441 cccaaagaat cgccgaccag cagcatttcc acgccggcat cgtccatcag cgcggcaaaa
    13501 ctggattcgt aagcggtcag catagcgatt ttttcgcccg ccgccttcat tttttgcagt
    13561 gtgttcacag taatcatcag atattgttac cccgcccttt tcagacggcc ttttcagttt
    13621 gaaacgggct attatagtga aatgcccgca gccctgccac cgaccgtgcc gcaaatgcaa
    13681 aacagcccga ctgttttaca caatcggact gtcaaatctg gtgccggcac caagagtcga
    13741 actcgggacc ccctgattac aagtcaggtg ctctaccaac tgagctatac cggcttactg
    13801 aaatgttttg caacaccatc agcagaaatg gtgccggcac caagagtcga actcgggacc
    13861 ccctgattac aagtcaggtg ctctaccaac tgagctatac cggcaaagaa agcgaattat
    13921 gcaggcaatg ccgcaccttg gcaaggaatt tatccgaaag ccccggcaaa tccgaataag
    13981 taatttattt gaaaagaaat taatttgaaa cagaacaagc atcggcattc tgataaaata
    14041 tccgcctttc aaaccgaccg ttcacaccga tggcaagaca tccctaccgc cgcctgcgtc
    14101 ctgcaaaatc cggctttccc gaagtcggca tttccgaaga aggcaatatc cgttcgcttc
    14161 acttgggcag cgacaccgtt caaagctcga tgaacctcga ccacccgtcc gagctggtgc
    14221 tctcttacag ccgcgcgatg atgggctggc tgctgtttac cgacgctctt ccgcaacata
    14281 tcacccaaat cggcttgggc ggcggctcgt ttgcacgctg gatagatacc tacctgcccg
    14341 acacgcgcca aaccgccgtg gacatcaatc cgcaggtcat cgccattgcc cgcaacctgt
    14401 tcgagttgcc tttcgagggc gagaaatttg aaattattga agcagacggt gcagaatata
    14461 tcaaagtctt ccgccacaac accgatgtga ttttggtgga cgggttcgac ggcgaacaaa
    14521 tcatcgatgc gctggttgaa gaaccgttct tccgagactg ccgcaacgca ctctcttcag
    14581 acggcatatt cgtaaccaac tggtggagcg gcgacaaacg ctaccaacgc ttcatcgaac
    14641 ggttgttgag cgtttttgaa gggcgcgtct tggaacttcc tgccgaaagc cacggcaatg
    14701 tcgcggtaat ggccttccaa agcagcccca aagagcaaaa catagacaaa ctcaaaaaac
    14761 gtgccgacaa actgagcaac gcatacggat tggacttcca ccgtatgctt gccggcctga
    14821 aagcgtccaa ccccaacaac ggcaagcatt tccaccttta agcaactatc ttcacggctg
    14881 catcggaaca tgcggaaaat caagaggaaa tcattatgct gaaacccgaa accatacaca
    14941 tatccggtcc tgccggcatt ttagaaacca tccatatccc gtccgaacaa gtaccggcac
    15001 gcggtgttgc cgtcatcaat catcccaacc ccctccaggg cggaacaaac accaacaaag
    15061 tcatccaaac tgccgccaaa gccttaagca aactcggctt ccactgctac ctgcccaacc
    15121 ttcgcggcgt aggcggcagc ggaggcacac atgattacgg acgcggcgaa acgcaagact
    15181 gcctcgccgt catcgattat gcccgcgccc aacaccccga agcccccgaa tttgccttat
    15241 ccggcttctc cttcggcggt tatgtcgcca catttgccgc acaagcgcgc acacccgatt
    15301 tattgctgct catcggcgcg gcagtctgcc actataccga ccgtccggaa ccgtccgccg
    15361 ttcccaacgt tgccaaaacg ctgatgatac acggcgcgga agacgaagtc gtcgaaatcg
    15421 ggaaagcact gaaatgggcg gaaccgcaag atttgcccgt cattaccatc gccggttcca
    15481 cgcatttctt tcacggcaaa ctcatcgtcc tgcgcgacac catcctccgc ttcgcgcccg
    15541 tatgcctgaa cggataaaac ccctttcaga cggcatcgat aatgtaaaac ccgtatcggc
    15601 ggcatccgcc gatacgggtt tgcgttccct ctcattatag gcaaaatccg ctgcaaaaca
    15661 ccctttcccg acccgacaaa ggatctgcaa acgcaatctt ttttgccaat agcttcaaag
    15721 gtttccgata atcctcgctg cccgcttcag acggcacggg atagagcgcg tcattcaaca
    15781 gcggcatacc caaacccatc atatgcacgc gcaactggtg tttcttgccc gtatgcggcg
    15841 taaggcggta aaggctgaat tcccccctgt tttcaatcag ttcgaccgtc gtatgtgcgt
    15901 ttggttcgcc ttccgcctct tgcgtcgtaa aaaatttctc acccctcacc aaacgcgaaa
    15961 ccacatcgag cggatacggc aaatccgtcc ttgtcggcgc aagcgcctca tacgttttcc
    16021 ataccgtttt gttttgaaac atcgtctgat aggctccgcg cgtggcagga ttgtgcgaca
    16081 gcagcatcac gcctgccgta tccttgtcca agcggtgcag cggcgtaatg tcctcaacat
    16141 tcaaatgctg caattcaggc cgcaaacgca ggcgcgtgag cagggtttcc cgcaaaaacc
    16201 tgccgctggg gatgacgggc agaaaatgcg gtttgtccac cacaatcaaa tgctcatcaa
    16261 tatgcaaaat cttttcttca aacggaatac gcggctcgct ctcacggctg gtttcacggt
    16321 aataaaacat caccttaccc ggctcgaaca aagaatgttc gtccaacgcc gcaccatccg
    16381 aaccgaccac aaaaccgctg ttcaaccgcc tgcgccaatc gtccgcgccc acaaaaggaa
    16441 agcggatgca cagaaaatgc agcagcggca gcccgtaaaa ctgcttttca tgcggcagca
    16501 ccaaataact gggtttgaca ccgttcaaca gcggaagagg attattgcgt tttttcatgc
    16561 ggcggattgt aaccgttttt cacaaacttt tcagacggca tatcgaaaca taattgacaa
    16621 aaacaaacat attgtttttt atgatttaaa aatttattta gacaagatta tcggatattc
    16681 attattatca gtaaataaga attaatatca ataggagaaa tatgaagcgc atctttttgc
    16741 ccgccttgcc cgccatcctg cctttatcca cttatgccga cctgcccttg acgattgaag
    16801 acataatgac cgacaaggga aaatggaaac tggaaacttc ccttacctac ctgaacagcg
    16861 aaaacaaccg cgccgaactt gccgcaccgg tttacattca aaccggcgca acctcgttta
    16921 tccccattcc gaccgaaatc caagaaaacg gcagcaatac cgatatgctc gtcggcacgc
    16981 tcggtttgcg ctacggactg accgggaata ccgacattta cggcagcggc agctatctgt
    17041 ggcacgaaga acgcaaactc gacggcaacg gcaaaacccg caacaaacgg atgtccgacg
    17101 tatccctcgg catcagccac actttcctta aagacgacaa aaaccccgcc ctaatcagct
    17161 ttcttgaaag cacggtttac gaaaaatcgc gcaacaaagc ctcgtcggga aaatcctggc
    17221 tcatcggcgc caccacctac aaagccatag atccgattgt cctttccctc accgccgcct
    17281 accgcatcaa cggcagcaaa accctttcag acggcatccg ctacaaatcg ggcaactacc
    17341 tgctgctcaa ccccaacatc tcatttgccg ccaacgacag aatcagcctg accggaggca
    17401 tccaatggct gggcacacag cccgaccgca tagacggcaa aaaagaaacc gcaagaaaca
    17461 catccaccta cgctcatttc ggcacaggtt tcggtttcac caaaaccacg gctttaaacg
    17521 catccgcacg tttcaacgtt tcagggcaaa gcagttccga actgaaattt ggcgtacagc
    17581 atacatttta agcaggtttt gattttccaa ccttgtaatc caaaggagtt attatgaaaa
    17641 aacaaatcag ctcagccgta atgatgctgt ctatgattgc ccccgcaatg gcaaacggct
    17701 tggacaatca gacatttgaa aaccaagtgt tccacacgca ggcagatgcg ccgatgcagt
    17761 tggcggagct ttctcaaaag gagatgaagg agactgaggg ggagtggatt cctaatgctg
    17821 ttggaggcat tatgggagga gttggagggc atttcagcta tatggctagt gctattgcta
    17881 gtggctcgta taatcgaaat gaacattgga cagcaattgg aggtggtgcg ttaattggtg
    17941 cgatgaaccc tattaacgga ggaagagcac tgattaatgg tatgcgaggt gtagcagtgg
    18001 ggactgttgc tggtggcata aatggttatg caggttctac gggtaagaac actgacattc
    18061 gccgttaaat aataaataaa ccaacttcca accaaacctg ctatcggttt ggttgga
//
LOCUS       DAWGRZ010000022        18006 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000022 DAWGRZ010000000
VERSION     DAWGRZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18006
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.022"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..129)
                     /locus_tag="WHT44_000532"
     CDS             complement(<1..129)
                     /locus_tag="WHT44_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HEZ2532753.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAE"
     gene            374..1282
                     /gene="hslO"
                     /locus_tag="WHT44_000533"
     CDS             374..1282
                     /gene="hslO"
                     /locus_tag="WHT44_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ2532754.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGQGRLKMLVAEATSDRTVRATARWDET
                     AEIADDESLGDLLGEGGVFVLTLQPKDGEPWQGVVPLEGDGIAQMLVNYMKRSEQLDT
                     HIVLSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAEELAGLDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            complement(1368..2822)
                     /gene="mgtE"
                     /locus_tag="WHT44_000534"
     CDS             complement(1368..2822)
                     /gene="mgtE"
                     /locus_tag="WHT44_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ2532755.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITIDEMVDVIREESEADMLNMAGLQEEEDLFAPVWDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(3054..7346)
                     /gene="mspA"
                     /locus_tag="WHT44_000535"
                     /gene_synonym="ausI"
     CDS             complement(3054..7346)
                     /gene="mspA"
                     /locus_tag="WHT44_000535"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
                     /protein_id="HEZ2532756.1"
                     /translation="MRFTHTTPFCSVLSTLGLFAVSPAYSSIVRNDVDYQYFRDFAEN
                     KGAFTVGASNISIQDKQGKILGRVLNGIPMPDFRVSNRQTAIATLVHPQYVNSVKHNV
                     GYGSIQFGNDTQNPEEQAYTYRLVSRNPHPDYDYHLPRLNKLVTEISPTALSSVPLLG
                     NGQPKANAYLDTDRFPYFVRLGSGTQQVRKADGTRTRTAPAYQYLTGGTPLKVLGFQN
                     HGLLVGGSLTDQPLNTYAIAGDSGSPLFAFDKHENRWVLAGVLSTYAGFDNFFNKYIV
                     TQPEFIRSTIRQYETRLDVGLTTNELIWRDNGNGNSTLQGLNERITLPIANPSLAPQN
                     DSRHMPSEDAGKTLILSSRFDNKTLMLADNINQGAGALQFDSNFTVVGKNHTWQGAGV
                     IVADGKRVFWQVSNPKGDRLSKLGAGTLIANGQGINQGDISIGEGTVVLAQKAASDGS
                     KQAFNQVGITSGRGTAVLADSQQIKPENLYFGFRGGRLDLNGNNLAFTHIRHADGGAQ
                     IVNHNPDQAATLTLTGNPVLSPEHVEWVQWGNRPQGNAAVYEYINPHRNRRTDYFILK
                     PGGNPREFFPLNMKNSTSWQFIGNNRQQAAEQVAQAENARPDLITFGGYLGENAQTGK
                     AAPSYSKTNEAAIEKTRHIANAAVYGRPEYRYNGALNLHYRPKRTDSTLLLNGGMNLN
                     GEVLIEGGNMIVSGRPVPHAYDHQAKREPVLENEWTDGSFKAARFTLRNHARLTAGRN
                     TAHLDGDITAYDLSGIDLGFTQGKTPECYRSYHSGSTHCTPNAVLKAENYRALPATQV
                     RGDITLNDRSELRLGKAHLYGSIRAGKDTAVRMEADSNWTLSQSSHTGALTLDGAQIT
                     LNPDFANNTHNNRFNTLTVNGTLDGFGTFRFLTGIVRKQNAPLKLEGDSRGAFQIHVK
                     NTGQEPQTTESLALVSLNPKHSHQARFTLQNGYADLGAYRYILRKNNNGYSLYNPLKE
                     AELQIEATRAEHERNQQAYNQLQATDISRQVQHDSDATRQALQAWQNSQTELARIDSQ
                     VQYLSAQLKQTDPLTGILTRAQNLCAAQGYSVDICRQVAKAADTNDLTLFETELDTYI
                     ERVEMAESELDKARQGGDAQAVETARHAYLNALNRLSRQIHSLKTGVAGIRMPNLAEL
                     ISRSANTAVSEQAAYNTGRQQAGRRIDRHLTDPQQQNIWLETGTQQTDYHSGTHRPYQ
                     QTTNYAHIGIQTGITDRLSVGTILTDERTNNRFDEGVSARNRSNGAHLFVKGENGALF
                     AAADLGYSNSRTRFTDYDGAAVRRHAWDAGINTGIKIDTGINLRPYAGIRINRSNGNR
                     YVLDGAEINSPAQIQTTWHAGIRLDKTVELGQAKLTPAFSSDYYHTRQNSGSALSVND
                     RTLLQQAAHGTLHTLQIDAGYKGWNAKLHAAYGKDSNTARHKQAGIKIGYNW"
     gene            complement(7660..8418)
                     /gene="gloB"
                     /locus_tag="WHT44_000536"
     CDS             complement(7660..8418)
                     /gene="gloB"
                     /locus_tag="WHT44_000536"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ2532757.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            complement(8513..12475)
                     /gene="purL"
                     /locus_tag="WHT44_000537"
     CDS             complement(8513..12475)
                     /gene="purL"
                     /locus_tag="WHT44_000537"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ2532758.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFVG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGRLNDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSGVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGGKNPIISIHDVGAGGL
                     SNAFPELVNDARRGAVFKLREVPLEEHGLNPLQIWCNESQERYVLSILEKDLDAFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPVDLPLNVLLGKLPKTTRTDKTVAPSKK
                     PFHAGDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGLTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVKDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYSVIQQLVAENKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNDRSALFADVKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFADPDTLTLGVCNGCQMVSNLAEIIPGAEAWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNP
                     NGSPQGIAGVTNADGRVTIMMPHPERVYRAAQMSWKPEDWTELSGWYRLFAGARKALG
                     "
     gene            12626..12964
                     /locus_tag="WHT44_000538"
     CDS             12626..12964
                     /locus_tag="WHT44_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ2532759.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKIKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERSDAAV"
     gene            complement(13030..14118)
                     /locus_tag="WHT44_000539"
     CDS             complement(13030..14118)
                     /locus_tag="WHT44_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin NadA"
                     /protein_id="HEZ2532760.1"
                     /translation="MKHFPSKVLTTAILATFCSGALAATSDDDVKKAATVAIVAAYNN
                     GQEINGFKAGETIYDIGEDGTITQKDATAADVEADDFKGLGLKKVVTNLTKTVNENKQ
                     NVDAKVKAAESEIEKLTTKLADTDAALADTDAALDETTNALNKLGENITTFAEETKTN
                     IVKIDEKLEAVADTVDKHAEAFNDIADSLDETNTKADEAVKTANEAKQTAEETKQNVD
                     AKVKAAETAAGKAEAAAGTANTAADKAEAVAAKVTDIKADIATNKADIAKNSARIDSL
                     DKNVANLRKETRQGLAEQAALSGLFQPYNVGRFNVTAAVGGYKSESAVAIGTGFRFTE
                     NFAAKAGVAVGTSSGSSAAYHVGVNYEW"
     gene            complement(14406..15164)
                     /locus_tag="WHT44_000540"
     CDS             complement(14406..15164)
                     /locus_tag="WHT44_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ2532761.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLAGTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTREHKRTTVAVLHDINQAAAYADYVVAMKNGQVAMQG
                     KPNDIFTAENIKTLFDMDVNVLDYEGKKLVIHHI"
     gene            complement(15249..15875)
                     /locus_tag="WHT44_000541"
     CDS             complement(15249..15875)
                     /locus_tag="WHT44_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532762.1"
                     /translation="MTQEHFPSFFNQAPTITVRDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGARDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRHLLSFGVGEINGTLTLRRKDNGKTVAVGLNAALQPFAPE
                     MRDIMPKAVGGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            complement(15920..16894)
                     /locus_tag="WHT44_000542"
     CDS             complement(15920..16894)
                     /locus_tag="WHT44_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ2532763.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCVLFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTLGGV
                     GYTSLPAVGKFGFELAVMMGGSLLLFYTLVKQGGRDLSRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFASFNTVHNELLGIGALILLISAAAVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            complement(16884..17852)
                     /locus_tag="WHT44_000543"
     CDS             complement(16884..17852)
                     /locus_tag="WHT44_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ2532764.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIISRLMGDKLRQSLPAVALLG
                     ASLVLLCDIVGRVIVFPFEIPVSTVFGVMGTALFLWLLLRKPAHAV"
BASE COUNT         3633 a         4486 c         5461 g         4426 t
ORIGIN      
        1 ttcggcaaac tgtctgagaa tctgctcgcg gctgctgagc aggttggtca actcgtcggc
       61 aagggcgggg cggacggcaa acatcagcca caaaaccgcc caaactgccg gtttgaaaaa
      121 agaatccata tcggtgtttc cgcgcaggag gcatcgtgcc gccgtgcgat gttatgctgt
      181 atcagtcgaa aatatcttgt tgattgtata taaaaaaacg gctgtttggc aaaaaacggc
      241 ttgttagatt gagttaatat gttgattttt atataattta ttgataattt gggaagcgca
      301 tccgccgtcc gtcttgtttt cgcgccgacc gcaaccatat agccgccatc cgaacaatca
      361 gtcagagaaa atcatgaatc aaaccgccat caaccgcgcc gatgtgcgta cccgctttat
      421 cttcgacgat atgcccgtgc gcgggctgca cgtccgtctg gaaaacgtgt ggcagcacat
      481 tgtaaaacag aaaaactatc ccgccgccat ccgccgcgcc ttgggcgagc tgttggctgc
      541 cggtgttctg ctgtcgggca acctcaaaaa cgaaggcacg ctgattgtgc aggttcaggg
      601 gcaggggcgg ctgaaaatgc tggttgcgga agcgacttcc gaccgtaccg tccgtgcgac
      661 cgcgcggtgg gacgaaaccg cagaaatagc cgatgacgaa agcctcggcg atcttttggg
      721 cgagggcggc gtattcgtgc tgacgctgca gcctaaagac ggcgaaccct ggcagggtgt
      781 agtgcctttg gaaggcgacg gtatcgcgca aatgttggtg aactatatga aacgttccga
      841 acagcttgat acgcacatcg tcctgtctgc aagcgacgaa gcggcgggcg gtctgctggt
      901 gcagcgtctg cctgaagagg tattggatga agaggcatgg gaacacgtca gtacgctggc
      961 gcgcacgctg acggcggagg agctggcagg actggacgcg caacacgttt tataccgcct
     1021 gttccacgaa acgccgccgc gcgtgttcga gccggaaacg tttgaatttt catgcacctg
     1081 ttcgcgcggc aaagtcagcg atatgctgtt gatgctgggc ggggaagaag tcggcggcgt
     1141 ggtggtggaa caaggcagca tcgaagtcga ttgcgatttc tgccacagca aatatgtgtt
     1201 tgacgaaacc gatgtcaacg cgctgttcgg ggaggatgtg gtcggcgttg ccaaagggct
     1261 gccccggcat accgtccaat aatcttgtgc aacaaggata aatagtcaaa tgccgtctga
     1321 agcagcttcc gcttcagacg gcatttttgc gcgggttcag acggcattta aagcaggaat
     1381 agggtggcga gccccaagaa aatcaggaag ccgccggagt cggtaacggc ggtaatcagc
     1441 accgagctgc ccagtgcggg atcgcgtccg aacttttcca ttaccacggg aattaatacg
     1501 ccgacggttg ccgccagcag gaggttgagc gtcatcgcgg caatcataac cagcccgatg
     1561 ccgaggctgc cgtaaagcag ccaagatact gcgcccatga ccgttcccca aatgatgccg
     1621 ttgaccaagg cgacaccgac ttcttttttc agcaaacgcc ccgcctgcat atccgtcagc
     1681 tgccccatcg ccatcgcgcg gacaatcatg gtaatcgtct ggttgcccga gttaccgcct
     1741 atgccggcga cgatgggcat cagcgcggcg agtgcgacga ttttttcgat gctgccttca
     1801 aacgcgccga taacacggct ggcgaggaag gcggtgcaga ggttgacggc gagccacatc
     1861 cagcggtttt tcaccgaatc ccacacgggg gcgaacaggt cttcctcttc ctgcaaaccc
     1921 gccatattca gcatatccgc ttccgattct tcgcggatca cgtccaccat ctcgtcgatg
     1981 gtaatcctgc cgatgagctt tttgttttca tcgacgacgg gcgcggtaac caagtcgtag
     2041 cgttcaaacg cctgcgccgc ttcttccacg tcatcttcgg cgcggaaacg cacgacatct
     2101 ttcgccatca cgttttccac caagtcttcg ggatcggcga ccaaaagttt gcggatgggc
     2161 agcacgccct gcagtacgtc gttttcatcg accacaaaaa tcttgtcggt atggtcgggc
     2221 aggctgtcga agcggcgcag atagcgcagc accacttcac aggcgacatc ggcgcggatg
     2281 ctgaccaact cgaagtccat aatcgcaccg acttggttgt cttcgtagga cattgccgct
     2341 ttgacttggg cgcgttcttc ctcatcgcgg gtttgtagcg cttcgtaaac cacttggtgc
     2401 ggcaaatcgt ctgccagttc cgccaattcg tccgcgtcca aatcatcgac cgctgccaac
     2461 aattcgtctt tgtccatcga ctcgatcagc gtttcgcgca ccgcgtcgga tacttccagc
     2521 aatacttcgc cgtcgtcttc cggtttgacc agaatccaga cgatattgcg ttcgcgcggc
     2581 ggtagcgatt ccaataccgc cgccacgtcg gcagggtgca gctcgctcaa gaggacggtc
     2641 agctcggtca gcttgtcgcg cagcggcgcg tcttcgagcg gtgtaccgtt ttcgatttgt
     2701 tcaaaagcag gttcgaggat ttcgcagagg gagtggacac ggtcgaaatc ggcggaaacg
     2761 cgttctacat cgttttcgat accgtcgttt tcaaggttcg gaggggttgg ttcgatgctc
     2821 ataaatgctc cgcccgccgt gtgcggggag gcattccggc gggatggtta ttgcgggttt
     2881 gaatcggatg gggtgttcag taaaaacgaa ctgggaaggt ccataataaa agcctgacgg
     2941 tacaggcgca gggttggaaa cggcactatt ctactccctt ttggaacggt ttactatttt
     3001 taacgcaaat gccgtctgaa acgcgggttc cagacggcat ttttatcggc ttgttaccag
     3061 ttgtagccta ttttgattcc tgcctgtttg tggcgggcgg tgttgctgtc tttgccgtaa
     3121 gcggcatgaa gtttggcgtt ccagcctttg tatccggcgt cgatttgcag ggtatgcagt
     3181 gtgccgtggg cggcttgctg cagtaaggta cggtcgttga cgctgagggc ggaaccgctg
     3241 ttttggcggg tatggtagta atcgctgctg aaggcggggg tcagcttggc ttgacccagt
     3301 tcgacggttt tatcgagacg gatgccggca tgccatgtgg tttggatttg cgccgggctg
     3361 tttatctctg cgccgtcgag tacgtaccgg ttgccgttgc tgcggtttat acggatgccg
     3421 gcatagggtc tgaggttgat gccggtatcg attttgatgc cggtgttgat gcctgcatcc
     3481 catgcgtggc ggcggacggc agccccgtca taatcggtaa atcgggtacg gctgttgctg
     3541 tagcctaaat ctgccgcggc aaagagtgcg ccgttttccc ctttgacgaa cagatgtgcg
     3601 ccgttgctgc ggtttcgggc ggatacgcct tcatcaaaac ggttgtttgt gcgctcatcg
     3661 gttaaaatcg taccgacact gagacggtcg gtgatgccgg tttggatgcc gatatgtgca
     3721 tagttggtag tttgttggta gggacggtgt gtgccgctat ggtagtcggt ttgttgcgta
     3781 ccggtttcca gccagatgtt ttgctgctgc ggatcggtaa ggtggcggtc gatgcggcgt
     3841 cccgcctgtt gccggccggt attgtaggcg gcctgttcgg aaacggcggt gttggccgac
     3901 cggctgatca gttcggccag gttcggcata cggatgccgg caacgccggt tttcaaactg
     3961 tggatttgtc gggacagacg gttgagtgcg ttcaggtagg cgtgccgggc tgtttcgacg
     4021 gcttgcgcat cgccgccttg ccgtgctttg tcaagttcgg attcggccat ttctacacgt
     4081 tctatatacg tatccagttc ggtttcgaag agtgtcaggt cgttcgtgtc ggcggctttg
     4141 gcaacctgac ggcagatatc gacactgtat ccttgtgcgg cacacaggtt ttgggcacgc
     4201 gtcagaatgc cggtcagcgg gtctgtctgt ttcaattggg cggacagata ttggacttgg
     4261 ctgtcgatgc gggcaagttc ggtttgactg ttctgccagg cctgtagtgc ctgcctggtc
     4321 gcgtcagagt catgttgaac ctgtctgctg atgtcggttg cctgtaattg gttgtatgcc
     4381 tgttggttgc gctcatgttc cgcacgcgtg gcttcaattt gaagttcggc ctctttgagc
     4441 gggttgtaca ggctgtatcc gttgttgttt ttgcggagga tgtagcggta ggcacccaaa
     4501 tcggcatagc cgttttggag ggtgaatcgg gcttggtggc tgtgtttcgg attgaggctc
     4561 acaagtgcaa gcgattcggt tgtttgaggt tcttgtccgg tgtttttgac gtggatttgg
     4621 aatgcgccgc ggctgtcccc ttccagtttg aggggggcat tttgttttcg gacgatgccg
     4681 gtcaggaatc ggaatgtgcc gaacccgtca agtgtgccgt tgacggtcag tgtgttgaag
     4741 cggttgttgt gtgtattatt ggcgaaatcg gggttcaggg taatttgtgc gccgtcaagc
     4801 gtcagtgcgc cggtgtggct ggactgggaa agtgtccagt tgctgtctgc ttccatgcgg
     4861 actgcggtgt ctttgccggc acggatgctg ccgtacaggt gtgctttgcc caggcggagc
     4921 tctgaacggt cgttaagggt aatgtcgccg cgtacttgcg ttgcaggtag tgcacgatag
     4981 ttttcggctt ttaaaacggc gttgggtgtg cagtgggtgc tgccgctatg gtaggagcgg
     5041 tagcattccg gtgttttgcc ttgggtaaag ccgaggtcga tgccggacag atcgtatgcg
     5101 gttatgtcgc cgtccagatg cgcggtattg cgccctgccg tcagtcgggc atggtttcgc
     5161 agggtgaacc gtgcagcctt gaagctgccg tcggtccatt cgttttcaag aacgggttcg
     5221 cgtttggcct ggtggtcgta ggcatggggt acgggcctgc ctgacacaat catattgccg
     5281 ccctcaatca agacttcccc gttaaggttc atgccgccgt tgagcaacag cgtgctgtcg
     5341 gtgcgtttgg gacgatagtg caggttgagt gcgccgttgt aacggtattc gggccggccg
     5401 tatacggcgg catttgcgat atggcgggtt ttttctatgg ctgcttcatt ggttttgctg
     5461 taactcggcg cggctttgcc cgtttgcgcg ttttcaccca agtatccgcc gaaggtaatc
     5521 aggtcggggc gggcattttc ggcttgggcg acttgttcgg cggcctgttg cctgttgttg
     5581 ccgataaatt gccagcttgt tgagtttttc atatttaacg ggaaaaattc gcgcgggttg
     5641 ccgccgggtt tgagtatgaa gtagtcggtc cgacggttgc ggtgcgggtt gatgtattcg
     5701 taaaccgccg cgttgccttg cggacggttg ccccattgca cccactcgac atgctcggga
     5761 ctgaggacgg ggttgccggt cagcgtcagt gtcgcggctt ggtcagggtt gtgattgacg
     5821 atttgcgcgc cgccgtccgc atggcggata tgggtaaagg caaggttgtt gccgttgagg
     5881 tcgagccgtc cgcccctgaa gccgaaatag aggttttcgg gtttgatttg ctggctgtcg
     5941 gcgaggacgg ccgtgcccct gccgctggtg atgccgactt ggttgaatgc ttgtttgctg
     6001 ccgtctgaag cagctttttg ggcgagtacg acagtgcctt ccccgatgct gatgtcgccc
     6061 tggttgatgc cttgtccgtt ggcgataagc gtgcctgcgc ccagtttgga gagccggtcg
     6121 cctttggggt tgctgacttg ccagaagacg cgtttgccgt cggctacgat aacgcctgca
     6181 ccttgccatg tgtggttttt accgacgacg gtgaagttgc tgtcgaactg caatgcgcct
     6241 gcgccttggt tgatattgtc tgccagcatc agtgttttgt tgtcgaacct gctggatagg
     6301 atgagcgttt tgccggcatc ttcagacggc atgtgcctgc tgtcgttttg tggggcaagc
     6361 gaagggtttg caatgggcag ggtgatgcgt tcgttgagcc cttgcagggt gctgttgcca
     6421 ttaccgttgt cgcgccatat gagttcgttg gtggtcagcc cgacatccag ccgggtttcg
     6481 tattggcgga tggtggaacg gatgaattcg ggttgcgtga cgatgtattt gttgaagaaa
     6541 ttatcgaagc cggcgtaggt gctgagtacg cccgcaagca cccagcggtt ttcatgcttg
     6601 tcgaaggcaa acagggggga accgctgtct ccggcgattg cgtaggtgtt aaggggttgg
     6661 tcggtcaggc tgccgccgac gagtaagccg tggttttgga accccaatac tttcagcggc
     6721 gtgccgccgg tcaggtattg gtatgccggg gcggttcgtg tacgcgtgcc gtctgctttg
     6781 cggacttgtt gcgtgcctga gccgagtcgt acaaagtagg ggaagcggtc ggtatcgagg
     6841 taggcattgg cctttggctg gccgtttcca agcaagggta cgctgctgag tgcggtaggt
     6901 gagatttcgg taaccagttt gttgaggcgg ggaaggtggt agtcgtagtc cgggtgcggg
     6961 ttgcgtgata cgaggcggta ggtataggct tgttcttctg gattttgggt gtcgttgccg
     7021 aattgtatgg aaccgtagcc gacgttgtgt ttgacactgt tgacgtattg ggggtgaacc
     7081 agggtggcga tggcggtttg gcggttgctg acgcggaagt cgggcatggg gatgccgttg
     7141 agaaccctgc ctaatatttt gccttgcttg tcttggatgg aaatattgct tgcacctacg
     7201 gtgaacgcgc ctttattttc ggcaaagtcg cgaaaatatt ggtaatcgac atcgttgcgg
     7261 acaatggatg agtaagcagg ggaaacggca aaaagaccga gggtggacaa tacggaacaa
     7321 aatggggtgg tgtgtgtgaa gcgcatgatg attatccgtg taatagagaa aatcaacgat
     7381 tcttgcggac aagaatcacc gatcccgttt tgtgctggat ttatatagtg gattaacaat
     7441 atagtggatt aacaaaaacc agtacagcgt tgcctcgcct tgccgtacta tctgcggctt
     7501 cgtcgccttg tcctgattta aattcaatcc actatattat atattttgtt tttgtttgat
     7561 tataaggcaa cggtgtcctt tcgtctattg gcgatgattg tatgatttta tttaagattt
     7621 taataaaaat cctaggaatg gctgcgtttt cccgttgttt tatttcgtcc ggtattggtt
     7681 tttaagttca cgcagcgcga caaaggtatc gaggctgctg tttaacgttt tcccgctcaa
     7741 tgcctcggcg cggtctctga cgtgcggcag gtcgacgcgc aaaaacggat tgacgcggcg
     7801 ttcgtgcgcg agggtaacgg gcagggtagg cgtatgcgcc gccgctttca gtgccgtctg
     7861 aatgtcggcg ttgtccggct cgatatgggc ggcgaaacgc aggttggcgg cggtgtattc
     7921 gtgcgccgga tagaacaggg tgttttcagg caggcggttg aagcgttgga agctgtcgta
     7981 aagctgttcg attgtgccgg taaacacgcg tccgcagccg gcggaaaaaa gggtgtcgcc
     8041 gcaaaagacg tgtatgccgt ctgaagtttc gagaaggtag ctggtgtggc ggtctgtgtg
     8101 gccgggtgtt gcccaaacgg taacctgtcc gtcgccgaag gtgaattggg tgccggcggt
     8161 tacggtgtgg gttgctgctt cgatgtcgga ttcgccgtaa acgggcgatt ccatgtagcc
     8221 gcgccagagt gccgccgcac cgccctcgtg gtcgggatgg gggtgagtta cccatgtttg
     8281 ggcaagcatg aggcggttgc ggacgaggaa ttccaagacg ggcgagggtt cggaagggtc
     8341 gacgcagacg gcatggttgc cgtgctgtat catccagatg tagttgtcgg ttagggcttt
     8401 gacgggggtg attttcatga gtgttcctcg gtggacggat accgtttaaa ggatggtatg
     8461 ccggaacgcc gtctgaaata ttcttcagac ggcattggtt tgagtagggc ggttagccca
     8521 aggctttacg tgcgccggca aagaggcggt accagccgga caattccgtc cagtcttctg
     8581 gtttccagct catttgtgcg gcacggtaca cgcgttccgg atgcggcatc atgatggtca
     8641 cgcggccgtc ggcgttggta acgccggcga tgccttgcgg cgagccgttg gggttgagcg
     8701 ggtaggtttg ggtcacttgg ttttgaccgt ccacatattg cagcgcgatg cccaaatcgg
     8761 cggaaatatt gccgccgtga agcgcgaagt cggcgcggcc ttcgccgtgg ctgaccacga
     8821 caggcaggct ggagccttgc atttcgttca gaatcagcga cgctgatttc ggaacgtgaa
     8881 ccatgctcag gcgtgcttca aactgttcgc tcaggttgcg tttgaattta ggccatgcct
     8941 ctgcacccgg gataatctcg gcgaggttgc tgaccatctg gcagccgttg cacacgccca
     9001 atgtcagcgt gtccggatcg gcaaagaagg cggcaaattg gtcgcgcaag gcagggtgga
     9061 acaggatgga tttcgcccag ccttcgcccg cgccgagtac gtcgccgtag ctgaagccgc
     9121 cgcacgccgc cagcattttg aagtcggcaa ggcggaagcg gcctgccatc aggtcggaca
     9181 tatgcacgtc gtaagcatcg aatccggcgc gggtgaaggc ggcggccatt tcgatttgcc
     9241 cgttcacgcc ctgttcgcgc aggatggcga ttttgggttt cgcgccgctg ttgataaacg
     9301 gcgcggcgat gtcttcgttc acgtcgaact tcacgtcggc aaacaatgcg ctgcggtcgt
     9361 tgtcgccaat cagtgcgaac tcgctgtcgg cgcaggcagg gttgtcgcgc aggcgttgga
     9421 tttgatggct ggtttcttgc caggtttgtt gcagtttgat taggttgtca gaaataaggt
     9481 gcgtttgccc gtcgcggatg attaacgtgt tctcatcagt taacgtaccg atttcaaaga
     9541 cattatgatg cagctgttgt tgatagaata aattgataat atcggcaaca tcttgttttc
     9601 taacttggat aacagcaccc aactcttcat taaataatgt gcgggcaatg gtttcttgcc
     9661 attcagccaa cgcttttacc tcttcagtcc gcaatgattg agacagagcg gtatggttgg
     9721 taataaatgt ttgtgcaagc aataaattta aatctatatc caagccgcac cgccccgcaa
     9781 acgccatttc taccaaaacg gcaaacaagc cgccgtcgct gcggtcgtga tacgccaaga
     9841 gtttgttttc ggcaacaagc tgctgaatca cactgtaaaa ggctttcaga cgacctgtat
     9901 cgtccaaatc gggcgcgtcg ccgctcatat tgttgtacac ctgaccaaac gccgaaccgc
     9961 ccatacgcgc tttgccgaag cccaaatcga caaacaacaa tacgctgtct tcgacgtttt
    10021 tcaactcagg cgtaacagtc ttgcgtacgt ctttcacagg cgcgaacgct gagataatca
    10081 ggctcaacgg tgaaaccacg gattttttct cctcgccgtc ctgccaaacg gttttcatcg
    10141 acaggctgtc tttgcccacg gggatgctca aatccaatgc ctgacaggct ttggaaacgg
    10201 cttcgacagt gcggtagagt ttttcgtctt cgccttcgtt gccgcacgcc gccatccagt
    10261 tggcggagag tttgatgttg ccgatgtctc cgatgttgac cgccgcgatg ttggtgatgg
    10321 cttcgccgac gcacattctg cccgaagcag gcgcatcaaa cagggcgacg gtcggttttt
    10381 cgcccataga catcgcttca ccgcgatagg tgttgaagcc catcatggta acggcgcagt
    10441 cggctactgg agtttgatat ttgccgacca tttggtcgcg gtgcgtcaaa ccgccgacgc
    10501 tgcggtcgcc gatggtaatc aggaagtttt tggcggctac ggcaggcagg cgcaaaacgc
    10561 ggtaggcggc ttcggtaatg tcgatatcgc ccgcgtgaaa cggttttttg gacggtgcaa
    10621 ccgttttgtc ggtgcgcgtg gttttgggca gtttgccgag caagacgttc aacggcaaat
    10681 cgacgggatt gttggcgaac aaatcgtcgc gtactttcaa atgaccgtcg tcagtcgccg
    10741 tgccgactac ggcaaacggg cagcgttcgc gttcgcagat ggcgcggaaa gcatccaaat
    10801 ctttttccaa aatcgacaac acataacgct cttgcgattc gttgcaccag atttgcagcg
    10861 ggttgaggcc gtgttcttca agcggcactt cgcgcagctt gaatactgcg ccgcgtctgg
    10921 catcgttgac cagttcgggg aaggcgttgg acaggccgcc cgcgcctacg tcgtggatgg
    10981 agataatcgg gtttttgccg ccgagctgcc agcagcggtc gatgacttcc tgcgcgcggc
    11041 gttcgatttc ggggttgccg cgttgcacgg agttgaagtc caaagacgcg tcgtttgtgc
    11101 cggtatccat cgaagaagcc gcgccgccgc ccaagccgat aagcataccc gggccgccca
    11161 gttggatcag caatgcgcct tcggggattt cgtctttatg cgtctgctgc gcctgaatgc
    11221 tgcccaagcc gccggcaatc ataatcggtt tgtgatagcc gcgaacctga ccgtcaaatt
    11281 tttcttcaaa agtgcggaag tagcccaaga ggttggggcg gccgaattcg ttgttgaacg
    11341 ccgcgccgcc gatcgggcct tcaatcatga tgtccagcgg cgaggaaata tgttccggct
    11401 tgccgtagtc ttgttcccac ggctgtttga ggtcgggaat attgaggttg gacacggtaa
    11461 agccggtcag gcccgctttc ggacgcgaac ctttgcccgt cgcgccttcg tcgcggattt
    11521 cgccgcccgc gcccgtcgcc gcacccgcaa acggcgcgat ggcggtcggg tggttgtgcg
    11581 tttccacttt catgatgata tgcgtgtctt cctcgtggaa acggtagcct tggttttccg
    11641 ccgcattcgg atagaaacgc tcgattttcg cgccttcgat tacggacgaa ttgtctttat
    11701 aggcaacgac cgtgccttcg ggatgcgcgt tgtgtgtgtc gcgtatcata ccgaagaggg
    11761 atttgggctg cttttcgccg ttgaggatga aatcggcgtt gaagattttg tggcggcagt
    11821 gttcgctgtt tgcctgcgcg aacatcatca attcaacatc ggacggattg cgctgcaaag
    11881 cctgatagtt ttcgaccaga taatcgattt cgtcggcgga aagtgccaag cccatttcgg
    11941 tattggcttt gaccaaagct tctttaccgc cgcccaaaac atcgacgccg gagaaagttt
    12001 cggattcgag atggtggaat aatttggagg ccgtctgaaa atcgggcagc acgctttcgg
    12061 tcatgcggtc gtgcagcaaa gccgcccatt gctgtttctg ttcatcgttc agacgacctt
    12121 ccagccacac cgccataccg cgctcgatgc gttcgatgcc tgccaaaccg cagttttccg
    12181 cgatattggt cgccttggaa gcccacggcg aaatcgtacc caaacggggc gtgaccaaaa
    12241 acaaatgcaa gccctcgcgc gcttttggcg tttgttcaac gctttgcgcc gccaacaagg
    12301 cttgcagttt ttcgacagtc gcggcatcaa gtgctttctc gctgccgacg aaataccaaa
    12361 attcgctgct taatttgact tcgggcagac cgagtgcggc ggctttttgc aagagttttt
    12421 caacacggaa atcggaaagg gcggtaacgc cgcgcaaggg caaaacgaca gacatggatt
    12481 cggctctcaa atgcggttgg aaaatctgtc attatacgcg caacggggct gtattgcttg
    12541 ggtattttgg cttaaaatgt tgacaatctg acgggaggtt cccgtttttc ggatgtgttt
    12601 gtattgtttg ttcaaaggaa attttatgaa aaaaatcgag gcgattgtca aaccgttcaa
    12661 actcgacgac gtgcgcgagg cgttgacgga aatcggcatt acgggcatga ccgtcagcga
    12721 ggtcaaaggg ttcggcaggc agaaggggca tacggaaatc tatcgcggcg cggaatacgc
    12781 cgtcgatttc ctgcccaaaa tcaaaatcga gctggtgttg gcggatgatg ctgtggaacg
    12841 cgcgattgac gtgattgtcg aggtggcgcg ttcgggaaaa atcggcgacg gcaagatttt
    12901 tgtgctgccg gttgaggagg caatccgtat ccgcacgggc gaacgttcgg acgcggcagt
    12961 ctgacggccg gatagaaaat aaaaaccgca gccttgcagg gctgcggttt ctttatcggg
    13021 agatgctgct taccactcgt aattgacgcc gacatggtag gctgcggaag aaccggacga
    13081 agtgccgact gccacgcctg ctttggcggc aaagttttcg gtaaagcgga agccggtacc
    13141 gatggcgact gccgattcgg atttgtagcc gccgactgca gccgttacat tgaaccgacc
    13201 cacgttgtaa ggttggaaca ggccggagag cgcggcttgt tctgcaaggc cttggcgggt
    13261 ttctttgcgc agattagcta cgtttttgtc caagctgtcg atgcgtgctg agtttttagc
    13321 aatatcagct ttgttcgtag cgatatcagc tttgatgtcg gtaacttttg cagcgacagc
    13381 ttcggccttg tcggctgcag tattagctgt gccagcggca gcttcggctt tgcctgctgc
    13441 agtttctgca gcttttactt tggcatcgac gttttgtttg gtttcttcgg ccgtctgttt
    13501 ggcttcattg gcggttttga cggcttcgtc tgccttagtg ttggtttcat ccaatgaatc
    13561 ggcgatatcg ttgaatgctt cggcatgctt gtcgacggta tcagccacgg cttctaattt
    13621 ttcatcaatt tttacgatat ttgtcttagt ctcttcagca aatgtcgtta tattttctcc
    13681 caatttattc aaggcgttgg tggtttcatc cagagcggca tcagtatctg ctaaagcggc
    13741 atcagtgtct gctaacttgg ttgttaactt ttctatttca gattctgcag cttttacttt
    13801 ggcatcgacg ttttgtttgt tttcattgac ggttttggtc aggttagtca cgactttttt
    13861 cagacccaga cctttaaagt cgtcggcttc aacatcggct gcagttgcgt ctttttgggt
    13921 aattgtgccg tcttcaccaa tgtcgtagat ggtctctcca gctttgaaac cgttgatttc
    13981 ttggccattg ttgtaggcag caacaatggc cacagtggca gcttttttaa catcgtcgtc
    14041 gcttgtggct gccagtgcgc cgctacagaa agtggcaagg atggctgtgg tcagtacttt
    14101 ggatggaaag tgtttcatgc tcattacctt tgtgagtgga taaaaaatgg ggcggaaatc
    14161 ttttgtaagt attttatccc caccaagttt gtttacatat taaagtatgt atgaaaggag
    14221 gcatatatac aatacattgt cgcaatttat ttatttattt atttaattct tgcagttagg
    14281 tagttcggca ttaatatctg ttaatatgtg catattatgc tgatgtcaat tctgccggtg
    14341 tcgtgtctta attttgtcgg gatatgggtt gccgtctgaa tgttcagacg gcctttttgt
    14401 cggatttaga tatggtggat aaccaatttt ttgccttcgt aatcgaggac gttgacgtcc
    14461 atatcgaata aggttttgat gttttcggcg gtgaaaatat cgttgggttt gccctgcatg
    14521 gcgacttggc cgtttttcat ggcgacgacg taatcggcgt aggcggcggc ctggttgatg
    14581 tcgtgcaata cggcgacggt ggtgcgtttg tgttcgcgcg tgagcttttg cagaatctgc
    14641 atgagcgagc gggcgtgata catatcgagg ttgttcagcg gttcgtccaa aaggacgtag
    14701 tcggtgcttt ggcagaacac catcgcaatc atggcgcgtt ggcgttgtcc gccggagagt
    14761 tcggtcagat agcggtcgga gaggtcctgc aggtggaatt cttcgattgc accgttaacg
    14821 atacggcggc attcggcagt cggtctgcct tgatggtagg ggtaacgtcc gaacatcagc
    14881 aggtcgcgca cggtgatgcg gctcatgatg ctgttttctt gggtgaggat ggagagcgtg
    14941 cgggcgagtt cggcggtggg agtgccggca aggtttttgc ctcggtaggc gatgctgccg
    15001 ctttcaagcg ggcgcagccg cgccataaag gaaaacaggg tggatttgcc tgcgccgttc
    15061 gggccgacga gggcggtaat gccgccttcg gggatgtcga ggctgacgtt gtcgaggatg
    15121 gggcgtgtgc cgatgcggta gctgacgttg cggatggtaa tcatggcgga tgcttgtcgg
    15181 gcgtaggttt gttgtatgtt tggcgggggt tcagacggca tttgaaattc ggaatgccgt
    15241 ctgaacgctc acacttcgcg gacgatgacg aactgcgggt cgtccgccga ttcggtcaaa
    15301 aatgctttaa cgcgtgcctg ccagagttgt ccgaagcgtt cgagttcttc tgcgcttgcg
    15361 ctgccgccga cggctttggg catgatgtcg cgcatttcgg gtgcgaaggg ttgcagggcg
    15421 gcgttgaggc cgacggcgac ggttttgccg ttgtctttgc ggcgcagggt cagtgtgccg
    15481 ttgatttcgc ctacaccaaa ggataagagg tggcggcggg caaagcgtcc ctgcattccg
    15541 atgccgccaa agccggtttc gggggctgcg ccggtgagga gttggacgac ggacgcggtt
    15601 acgccgaccg tgccttcgtc gcgcgctccc tgcatggcgg cttcgatgcc gccgcgttcg
    15661 ggcagctctt cgccgtaaag ggctttcaga cctttgataa ccatcaggta cgcgcccgcg
    15721 acggtcgggc aggaatgtcc gcacaggcgc acggcatcgg cgtagcggta agtgaggatg
    15781 ccgttttcgg ccgcgccgag gaatgcggca agcgggtctc taacggtgat ggtcggggct
    15841 tggttgaaga atgatgggaa atgttcttgt gtcatatggt ttgtcctttc gaggcgggcg
    15901 gccgttcaga cggcatccgt cattttttgt gttttaaaac gagatagagg aaaacgagtc
    15961 cgcccgcaaa ttcgaccacc acgcttaata ccgccttcat gcccaagaag tgttcgaata
    16021 cggtttgtcc gccgaccagg aggataccgc cgacacaaac cgtcatcggc aggcggacgg
    16081 aatggcgcac ggacggggaa aagtggttgg caagcgaggc ggcgagaagc ccgaaaaagc
    16141 tcaccgggcc gaccacggcg gtcgccgtcg ccaccagcgc ggcaatccaa agcagtatcc
    16201 ataaggtgtt gcgcgtgtag ctgatgccca aattgacggc ttggtcgcgc cccaaaaggt
    16261 gtacgtccaa gcggtagcgt tcgcgccaaa ccgccgccgc gctgatgagc agaatcagcg
    16321 cgcctatccc caacagttcg ttgtggacgg tattgaaact ggcgaacatg tttgcctgcg
    16381 ccgcggtaaa ctcttcggga tcgatcatgc gggagagcag cgaggaaagg ctgcggaata
    16441 aaatcccgaa aatcacgccg attaaaatca tgcgcgacaa atcgcgcccg ccttgtttga
    16501 cgagcgtgta gaacagcagc agcgagccgc ccatcatgac ggcaagttcg aagccgaact
    16561 tgccgaccgc aggcagggac gtatagccca cgccgccgag cgtaaacacc agcaaggtct
    16621 gcaaaaacac atacagtgaa tcaaaaccca aaatcgaagg ggtcagaatc ggattgttgg
    16681 tcagcgtttg gaagagttgc gtggacacgc cgaccgcata ggcgaccatc agcagcgcgg
    16741 caagtttggt cagccgcagg tgcaaaacaa aatcccaatc gcctttgacg ttgagcgtca
    16801 taaacaggac gcaggaaacc agcaacagcg caaaggcgac ccacaacgga cggctgcttc
    16861 ctgccataaa accgatattt ttttcagacg gcatgagcag gtttcctcaa caaaagccac
    16921 aaaaacaaag ccgtccccat cacgccaaaa accgtagaga ccggaatttc aaacggaaac
    16981 acaatcacgc gtccgacaat atcgcacagc aacaccaaag atgcgcccag caaggccacc
    17041 gcaggcaggc tttgtcgcag cttgtcgccc atcaggcggc tgatgatgtt cggcacgacc
    17101 agcccgataa acgggatatt gccgaccgta acgataacca gcgacgtaat caaagccaca
    17161 ataatcagcc ccgaccacaa caccgccgtc cggttcaaac ccaaattcac gctgaccgtc
    17221 tcgcccagcc ccaaaatcgt cagccggtcg gcaatcagat aggcaaacac cgccaaaccg
    17281 cccgtaatcc aaagcagctc gtaccgcccc agcagcacgc tcgaaaaatc gccctgctgc
    17341 cacacgccga gcatttgcag catttcgttt tcatacgcga taaacgtcgc cactgcctca
    17401 accacgccgc cgaaaataat ccccaccagc ggcaccatca gttgcgccgt cggcggcaga
    17461 cggcggatca gcagcataaa caccagcatc ccgatcagcg cggcaacggc ggcaaccgac
    17521 attttgaccg gcagcggcgc ggcaggcagc agcagggaca tcagaagcaa acccaaagcc
    17581 gcgctttgac ccgcgcccgc catagaaggc tcgacaaaac ggttacgcat caaaatctgc
    17641 ataatcatcc ccgccaccgc catcgacgcg cccgtcagca caatcgcaaa cgtgcgcggc
    17701 aggcggctga tgaacataac ctgctggctg tccgacagcg aaaacacatc cgaccagcgg
    17761 aaatcggcaa cgccgaccga caggctgacg gcaaacaaca ccgccagcag caggaggttg
    17821 gtcaggttga gggaaaaagg tttggcagtc ataaacagaa gggaaaagcg ttaaggcgta
    17881 gaagattcaa acaaggcagt ccgaaccgtc ggagcggaaa gccttgtttg aagcctccgt
    17941 atcgggcaga ggatagggca agttccaata tagtggatta acaaaaacca gtacggcgtt
    18001 gcctcg
//
LOCUS       DAWGRZ010000023        17158 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000023 DAWGRZ010000000
VERSION     DAWGRZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17158
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.023"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..174)
                     /locus_tag="WHT44_000544"
     CDS             complement(<1..174)
                     /locus_tag="WHT44_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532765.1"
                     /translation="MPSERLSDGIFPCVLNRIHETPDAFGGNVGCRAMFGGDQRTKYA
                     GKGLMPSEGSDGIG"
     gene            complement(192..749)
                     /locus_tag="WHT44_000545"
     CDS             complement(192..749)
                     /locus_tag="WHT44_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ2532766.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(825..2459)
                     /locus_tag="WHT44_000546"
     CDS             complement(825..2459)
                     /locus_tag="WHT44_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ2532767.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2533..2862)
                     /locus_tag="WHT44_000547"
     CDS             complement(2533..2862)
                     /locus_tag="WHT44_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532768.1"
                     /translation="MSRCCLKRLQTASAVSAFSGGVLEVVRLCCRRLSVLFNPSVMLF
                     VPDCPVGGAAGFFQYEMLCPFFWQGRLQTGSNLAYDVFMSAGRLNGGRNPPHSRRFLA
                     LLCSVAL"
     gene            complement(2862..3968)
                     /gene="serC"
                     /locus_tag="WHT44_000548"
     CDS             complement(2862..3968)
                     /gene="serC"
                     /locus_tag="WHT44_000548"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ2532769.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPCLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHINRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSDGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4333..4764
                     /gene="rimP"
                     /locus_tag="WHT44_000549"
     CDS             4333..4764
                     /gene="rimP"
                     /locus_tag="WHT44_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ2532770.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKESGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4792..6294
                     /gene="nusA"
                     /locus_tag="WHT44_000550"
     CDS             4792..6294
                     /gene="nusA"
                     /locus_tag="WHT44_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ2532771.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6306..9194
                     /gene="infB"
                     /locus_tag="WHT44_000551"
     CDS             6306..9194
                     /gene="infB"
                     /locus_tag="WHT44_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ2532772.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNSSTISIRRTKTEVSTVDGVKVETRKRGRTVKIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSEKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGLNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAINDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARSL"
     gene            complement(9356..10696)
                     /locus_tag="WHT44_000552"
     CDS             complement(9356..10696)
                     /locus_tag="WHT44_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HEZ2532773.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVKNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQLPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLIDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKVPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERREYAEKLGIRLAGK"
     gene            complement(10770..12110)
                     /locus_tag="WHT44_000553"
     CDS             complement(10770..12110)
                     /locus_tag="WHT44_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ2532774.1"
                     /translation="MLNPSRKLVELVRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRETLERVRAGSFWLWVVAATFAFFTGFSVTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPS
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWVVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIILNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVATNREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALAECGAAWLEPDRAAQEGRLKDQ"
     gene            12253..12879
                     /locus_tag="WHT44_000554"
     CDS             12253..12879
                     /locus_tag="WHT44_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ2532775.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYIVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(12943..14361)
                     /locus_tag="WHT44_000555"
     CDS             complement(12943..14361)
                     /locus_tag="WHT44_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ2532776.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWLSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIVALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAVRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYPYEFHEFHDKG"
     gene            14688..15416
                     /locus_tag="WHT44_000556"
     CDS             14688..15416
                     /locus_tag="WHT44_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ2532777.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     SNPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15475..15963)
                     /locus_tag="WHT44_000557"
     CDS             complement(15475..15963)
                     /locus_tag="WHT44_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ2532778.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCAAIVLMCRPRRKAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSVAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(16654..17118)
                     /locus_tag="WHT44_000558"
     CDS             complement(16654..17118)
                     /locus_tag="WHT44_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEZ2532779.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT         4511 a         4798 c         4335 g         3514 t
ORIGIN      
        1 accgatgccg tctgaacctt cagacggcat gagacctttg cctgcgtact tggtacgctg
       61 gtcgcctccg aacatggcgc gacacccgac atttccgccg aacgcatcgg gcgtttcatg
      121 aatccggttt aaaacgcatg gaaaaatgcc gtctgaaagc ctttcagacg gcattgtgct
      181 tgagattccg tttaccaatg gctgacaaac gcttccaaat cggtattctt gggcttatgc
      241 acttcctctg tcggcgtgcc gaccatcatc agcccgatga ttttatcctt atccgcacaa
      301 ccgaaagcct cccgcaacag ggggctattg acccacatcc ccgtaatcca gacattgtcg
      361 aatccctgag ccgttgccgc cagttgcagc gcatacgccg cacaacccgc cgtcagcatc
      421 tgctcccatt ccggtttcgg cttaggcaca tcgcggttcg gcgcaaacgt taccccgata
      481 accatcggcg ccatattgcc cactttttcc gcctttttca tcgcatcgtc gccgaaattc
      541 aattcggcaa ccgtttgctt caacacatcg cgaaaacgtt gcaatcctac ctcgccttga
      601 atcacggtaa aacggaaggg gcgcatattg ccgtgatcgg gaacttgggt tgccgcctga
      661 aatatttgtt ccaactccgc cgcatcgggg gcggggtgct tcagcttttt ggaagatcgg
      721 cggttcgtca ataattttaa agcatccata tcgttatact ccggtcatcg gtcgggtgtt
      781 cggacacata tgccgtccga aggcttcaga cggcatatcc ggcatcagcg cggacggcgg
      841 caggctgcca atatatccat ttccttccga taggtttggc tattggaaat gtccatcagc
      901 cccaataccg tgctgaaata gtggtcgtgc gaatattcgt tttccgccgc tttttgtttg
      961 aggcattgga aatctatgcc cccgtgttgg cggaaggctt tggaaaacca cataaccatc
     1021 gggatatgcg tctgcccgga aggcgcgatg gcgtaaggcg cggcgtgcag gtacatcccg
     1081 ttttcgccca aactttcgcc gtggtcggaa acataatgca ccacgctttc caaatcgtcg
     1141 cggttttcaa gtttgcggat aaccttgtcg ataaactggt ccacatacaa aaccgtattg
     1201 tcgtaagtgt tgaccagcgt ggcgcgggtg catttgttga tttcgttggt gtcgcaggtc
     1261 ggcgtgaatt tgcgttcggc ttcggtatag cgttcgtaat acgtcggccc gtggctgccg
     1321 atggtatgca ggattaaaac cgcgtcttta tcgtttttgt tgaggacttc gtcgaactta
     1381 gtcagcagga tattgtcgag gcactcgccg ttgcggcagt attcgggcag gttgagcgag
     1441 gtaacgtcgg tattcggcac tttgccgcac acgcccttgc agccggaatc gttttccaac
     1501 caagtaactt ccacgccggc gcgctgcacg atgtccagca ggttgtcttg gtgttcggct
     1561 ttgatttcgt cataatccgt gcggtcgaag gttgagaaca tacacggcag ggagtgcgcg
     1621 gtcgatgtgc cgcagcttct gacctgcggg aaattgacaa tttcatcgcc gcgcgcggca
     1681 agcagcggcg tagtttggcg gctgtaaccg ttcaaacccc agttggcggc acgcgtggtc
     1741 tcgcccacga ccagcaccac gaaacggcgc aggctgccgg ccgggcggtt ttgcacgacc
     1801 gccatatcca attgcgtata aggaatattg gaacgcttcc aatctttgta tttcgacacg
     1861 cccgcgccga tgaaattaga cggcacaatc agatgggtta ctgatttatt gttgcggaaa
     1921 aacgaggcgt aatcctgata ttgcaacatt gcgatgccca acgcgcacaa aaaggaaacg
     1981 gcggcaagca caaggcgcgt caaaagctcc ttataccaaa cgcggtattt aaccttgacg
     2041 gcgatatacg ccagcgcggg caatacgccc aaacatacaa tccacagcac atagcccggc
     2101 gtaatcaggc gcgcgctttc ggcagccgta gtttgcaaga cattattcaa catcgacttg
     2161 ttgaaataga tattgaaaaa tatttcttgg taagacaccg ccgcactgat aaccaatatc
     2221 aacggaatca ataccttatg cacgaaaggc agggcaatga cgtgaaaaac gaaattactt
     2281 aaaaaaaaca gcaccaccgg catcgtatag aggaagatat ccgccccggt gccgttaaaa
     2341 ggatgaagct cgacaacttt ggcaaaaaag gcgtaattca ataccagcga ggaatacagg
     2401 gaaaggaagg caatcagcgc ggaagagccg agcttcggcc tcaggttcgg ttttatcatt
     2461 tggaatgtgt cggataaggg ttggaaaagg catccggcat ttggaatccg gattattgaa
     2521 aaagattctt aattataagg caacggagca aagcagggca agaaaacggc ggctgtgcgg
     2581 ggggttccgc ccgccattca aacgtccggc agacataaaa acatcgtaag caagattcga
     2641 accggtctgc aaccgcccct gccagaaaaa cgggcaaagc atttcatatt ggaaaaaccc
     2701 agccgcgccg ccgacgggac agtccggcac aaacagcatc acgctcggat tgaaaaggac
     2761 ggatagccgc cggcagcaca atcttaccac ctccaaaacg ccgccggaga acgccgaaac
     2821 agccgatgcc gtctgaagcc gcttcagaca acatcgggac atcaaccgta acgccgttgg
     2881 aaatcgcgca taaaatctgc caaagcccgc acgccttcaa gcggcatcgc attataaatg
     2941 ctggcacgca taccgccgac ggttttatag cccttaagca ggcacaagcc ctgcaattcg
     3001 gcttccagca caaaacggcg gtcaagctcc tcatcccccg tttggaacac gacattcatt
     3061 ttagaacgcg cattcggacg gatacggttg atataaaaac catcgctgcc gtctatcgtc
     3121 tcatacaagg tttgcgcctt cagccgattg accgcttcaa tttttttcac accgccctgc
     3181 gcctgtagcc agcggaacac cagccccgac atataaatcg cgtaagttga cggcgtgttg
     3241 tacataccgt cgcggttgat gtgcgaacgg tagttgaaca catcgggaat atcgttcgga
     3301 caacgctcga gcaaatcctc acgcacaatc accaccgtaa ctcctgccgg cccgatgttt
     3361 ttctgtgcgc ctgcgtaaat cagtccgtag tcggcaacat caaactcgcg cgacaaaatc
     3421 tcgctggaca tatcgcacac cagcggcggc atgccttctg aaaggcacgg cacttcacgg
     3481 tattgcagcc cgttgaccgt ttcattgacg gcaaaatgga caaacgccga atcgggtgca
     3541 acatcccacg tttccacagg cggcaggtcg agatagtcga actgctcgcc gccatgcgcc
     3601 gccaaacgga tttccgtatc ggtcaaacgg ctcatctgtt cataagcgat acggctccag
     3661 ttgcccgtta ccaccgcgtc ggcagtgcgg aaaccgtgtg ccagattcat ggctgccata
     3721 ttaaattggg ttgttgctcc gccctgcaga aacaatatct tatagttgtc aggcactttc
     3781 aaaagctgcc tcaaatcctg ttccgcatga tgcaggatgc tcaaaaacat ttccgaacgg
     3841 tggctcattg ccatcacagg aaaacccgta ccgttgtagt ccaacatttc ctgccgcgcc
     3901 gtttccaaca cggcttcggg caatacggca gggccggcgg aaaaattgta aatcggataa
     3961 agagacatga tgcagccttg attctgaaca ataacccgcc cgattttagg cttgcagcag
     4021 gctttgtgca aggatggaaa atacctgtcc tccgcccgat tccatgccgc ccgaacacgg
     4081 aaaataatat caatatattg atttacaaac ataaaaatca tgcacgcgac aaatagatac
     4141 atttgttttg tcaacaatat tcacgatttc ccattacaaa cctcccttac acccgctttt
     4201 ttccgtccca aaaacacaaa ataaatcaac actttcattt ctccgcaaaa gcggttataa
     4261 tcacgccgat ttttcaactt tgacgaaaaa tggccacggg gccatttttt tattatgtac
     4321 atagggagca gcatggatat ccaaaccatc ctcgaaaaaa ccctgcccgg cctgggctac
     4381 gaactggtcg atttcgaact gaccgcgcaa ggaacattgc gcgtgttcat cgacaaagaa
     4441 agcggcatta ccgtcgaaga ctgcgcaacc gtcagcaacc acttgagccg cgtcttcatg
     4501 gttgaagaca tcgactacaa aaacctggaa atttccagcc ccggactcga ccgccccttg
     4561 aaaaaagccg ccgacttcgt gcgctttgcc ggtcagaatg ccaaaatcaa aacccgcctg
     4621 ccgatagacg gtcagaaaaa ctttatcggt aaaatcgaag gctgcgaaaa cgataccgtt
     4681 accgtatcct tcgacggcaa aaccgtacaa atcgaattgg gcaacatcga caaagcccgt
     4741 ctgcgccccg aattcaaatt ctaaaacaca acaatattgg agatgttcaa aatgagtcgt
     4801 gaaatgttac agctggcaga agcactggca agcgaaaaaa acgttgatgc ggaagtcgtc
     4861 ttccaagcac tggaattcgc cctgtctacc gccgccaaga aaaaggcaga ccgcgaacac
     4921 atggacgtgc gcgtccaaat caaccgcgac accggcgaat accaaacctt ccgccgctgg
     4981 ctgattgtcg ccgatgaaga ctatacctat cccgatgtcg aaaaaaccat cgaggaaatc
     5041 caagaggaaa ttcccggcac taccatccaa atcggcgaat actacgaaga gcagctgccc
     5101 aacgaaggct tcggccgcca agccgcgcaa accgccaaac aaatcatcct gcaacgcatc
     5161 cgcgatgccg agcgcgagca gaatctgaac gagtttctcg ccgtcaaaga agacatcgtg
     5221 tccggcacgg tcaaacgcgt cgaacgccac ggcatcatcg tcgaagtcgt tgccggcaaa
     5281 ctggacgcgc tgattccgcg cgaccaaatg attccgcgcg aaaacttccg cagcggcgac
     5341 cgcatccgcg ccctcttcct gcgcgtcgaa gaaatcggca acaccggccg caaacaagtc
     5401 attctgagcc gtacttccgg cgatttcctc gtcaaactgt acgccaatga agtacctgaa
     5461 attgcagacg gcatgcttga aatccgcgct gtcgcccgcg acccgggaca acgtgccaaa
     5521 gtcgccgtca aagccaacga ccagcgcatc gatccgcaag gcacctgtat cggcgttcgc
     5581 ggttcgcgtg tcaatgccgt cagcaacgaa ttgtccggcg agcgcatcga tgtcgtcctc
     5641 tggtcgcccg aacccgcgca attcgtgatg agcgcgctct cacccgccga agtcagccgc
     5701 atcgtcatcg acgaagacaa acacgccgtc gatgtcatcg ttgccgaaga ccagctcgcg
     5761 ctcgccatcg ggcgcggcgg tcaaaacgtg cgccttgctt ccgacctgac cggctggcag
     5821 ctcaacatca tgacttccgc cgaggcagac gaacgcaatg cggcagaaga tgccgccatc
     5881 cgccgcctgt ttatggatca cttgaacgtg gacgaagaaa ccgccgacgt actggttcag
     5941 gaaggttttg caaccttgga agaagtcgcc tatgttcctg ccgccgaact gcttgccatt
     6001 gaaggatttg acgaagaaat cgtcgatatg ctccgcaacc gcgcccgcga tgccatcctg
     6061 accatggcga ttgccgccga agaaaaactg ggcgaagtgt ccgacgatat gcgcaacctc
     6121 gaaggcatag atgccgatat gctccgcagc cttgccgaag caggcattac cacccgcgac
     6181 gacttggcag agcttgctgt ggacgaactg attgaaatca ccggtgtaaa cgaagaaacc
     6241 gcaaaagccg tcatcctgac cgcacgcgaa cactggttta ccgaagacaa ataaaggggg
     6301 tacagatgag taacacaacc gtagaacaat ttgccgccga gctgaaacgc cccgtcgaag
     6361 acctgttgaa acagttgaaa gaagccggcg tcagcaaaaa cagcggcagc gattccctga
     6421 cgctggacga caaacagctt ctgaacgcct acctgaccaa gaaaaacggc agcaacagca
     6481 gcaccatcag catccgccgc accaaaaccg aagtcagcac cgttgacggc gtaaaagtcg
     6541 aaacacgcaa acgcggacgc actgtcaaga ttccttctgc cgaagaattg gcagcacagg
     6601 taaaagccgc ccaaacccaa gccgcacctg tccggccgga gcagacggca gaagacgcgg
     6661 caaaagcccg agccgaagct gccgcacgcg cagaagcccg tgccaaggca gaagcggaag
     6721 cggcaaaact gaaagcggca aaagcaggca acaaagccaa acctgccgcg cagaaaccca
     6781 ccgaagcaaa agccgaaacc gcacccgttg cggcggaaac caaacccgcc gaagaaagca
     6841 aagcggaaaa agcccaagcc gacaaaatgc cgtctgaaaa acccgccgag cccaaagaaa
     6901 aagccgccaa gccgaaacac gagcgaaacg gcaaaggcaa agatgccaaa aaaccggcga
     6961 aacctgccgc acctgccgtg ccgcaacccg tggtcagcgc ggaagaacag gcgcaacgcg
     7021 acgaagaagc acgccgtgcc gccgcacttc gcgcccacca ggaagccctg ttgaaagaga
     7081 aacaggaacg ccaggcacgc cgcgaagcca tgaaacaaca ggcagaacaa caggcaaaag
     7141 ccgcacagga agccaaaacc ggcagacagc gtcccgccaa acctgccgaa aaaccgcagg
     7201 cagccgcgcc agccgtcgaa aataaacctg tcaatccggc aaaagcgaaa aaagaagacc
     7261 gccgcaaccg cgatgacgaa ggtcaaggcc gaaacgccaa aggcaaaggc ggaaaaggcg
     7321 gacgcgaccg caacaatgca cgcaatggcg acgacgagcg cgtacgcggc ggcaaaaaag
     7381 gcaaaaaact caaactcgag ccgaaccaac acgccttcca agcaccgacc gaacccgtcg
     7441 ttcatgaagt tttggttccc gaaaccatta ccgttgccga tttggcgcac aaaatggcgg
     7501 tcaaaggcgt ggaagtggtc aaagccctga tgaagatggg catgatggtt accatcaacc
     7561 aatccatcga ccaagacacc gccctgattg tggtggaaga actcggccac atcggcaaac
     7621 ctgccgcagc cgacgaccct gaagcattct tggacgaggg cgcggaagca gtggaagccg
     7681 aagcattgcc gcgtccgccc gtcgttaccg tgatgggcca cgtcgaccac ggcaaaacct
     7741 cgctgctgga ctacatccgc cgtaccaaag tggtacaggg cgaagcgggc ggcattacgc
     7801 agcacatcgg cgcgtaccac gttgaaaccc ctcgcggcgt gattaccttc ttggacaccc
     7861 cgggccacga agcctttacc gctatgcgcg cacgcggtgc gaaagcaacc gacatcgtga
     7921 ttctcgtggt cgccgccgac gacggcgtga tgccgcaaac catcgaagcg attgcccacg
     7981 ccaaagctgc gggtgtaccg atggtggttg ccgtcaacaa aatcgataaa gaagccgcca
     8041 acccagagcg tatccgccaa gagctgaccg cacacgaagt tgtgcctgac gaatggggcg
     8101 gcgatgtaca gtttatcgac gtttccgcta aaaaaggcct gaacatcgat gcattgctcg
     8161 aagccgtctt gctcgaagct gaagttttgg aactgaccgc acctgtcgat gcgcccgcca
     8221 aaggcatcat cgtcgaggcg cgcttggaca aaggccgcgg cgcggttgcc acattgctgg
     8281 ttcaaagcgg cacgctgaaa aaaggcgata tgctgctggc cggtacggca ttcggcaaaa
     8341 tccgcgcgat ggtcgatgaa aacggcaaat ccattaccga agccggtccg tccatccccg
     8401 tcgaaatcct cggcttgtcc gacgtaccga atgcgggtga agacgcgatg gtattggcgg
     8461 acgagaaaaa agcgcgcgaa atcgccctct tccgccaagg caaataccgc gacgtgcgcc
     8521 ttgccaaaca gcaggcggcg aagctggaaa atatgttcaa caatatgggc gaaacccagg
     8581 cccaatcttt gtcggtcatc atcaaggcag acgtgcaggg ctcttacgag gctttggcgg
     8641 gcagcctgaa aaaactgtcc acagacgaag tgaaagtgaa cgtgttgcac agcggcgtgg
     8701 gcggcattac cgaatcggat gtcaaccttg ccatcgcttc gggcgcattc attatcggct
     8761 ttaacgtgcg tgcagatgcc tcttcgcgca aacttgccga aaatgaaaac gtggaaatcc
     8821 gctactacaa catcatctac gatgccatca acgacgtgaa ggcggcgatg agcggtatgc
     8881 tttccccgga agagaaagaa caggttaccg gtacggtcga aatccgtcag gtcatctccg
     8941 tttccaaagt cggcaacatt gcaggctgta tggttaccga cggcgtggtc aaacgcgatt
     9001 cccatgtccg cctcatccgc aacaacgtgg ttatccacac gggcgaactg gcttcgttga
     9061 aacgctataa agacgatgta aaagaagtcc gcatgggctt cgagtgcggt ctgatgctca
     9121 aaggctacaa cgaaatcatg gaaggcgacc aactggaatg cttcgacatc gtcgaagttg
     9181 cccgcagcct gtaattcctt tgcaaataaa atgccgtctg aagcgttcag acggcatacg
     9241 aaacgggttc tgtatcatac agaacccgtt ttttgtcgca aatcggcttc agacagccct
     9301 cttgccttat cccgatttga atctgacttg ccatacaaac aggcttcaga cggcattatt
     9361 tgcccgctaa acgtatccca agcttctccg catattccct gcgttcggcg cggctggttt
     9421 ccgggcggtg cgtattgagc gacgaccatt tccaatgact gcgggctttg ttgagttcgg
     9481 gcgggagtct ggcggcatcc cacgggactt tgcggctgtg cagctcgata tccgactgtg
     9541 ccgcgtgtcc gcgcgtttgc aggacgtgga gcaaatcgag ggcgcgggcg gcgagcaggg
     9601 tcagggtttc agggtcggtg tgcagggttt ggcggccagc gagtttgtcg gaaatggtgc
     9661 gggtattggg caggatgccg cccaaaaagc cgccgattgc cgtacccaat ccgagcgagc
     9721 cgccgagtgt ggcgatgtcc agccccaagc cgatgagcgc gccggttgcc gcgcccgtgc
     9781 cggtgcggat gccgtattgt ttgagcaatt cgctgtcgaa cgggtcttgg cggaaggctt
     9841 gcggcatcca gtcgccgccg tcgatttcgc tgtggtagaa acggtagagg gcaaacagcc
     9901 gctgctgcat ctgccgttcg agttggcgta tttccgcctg catggtttgc agcacggtgg
     9961 cggtatcctc gttttcgtcc acttcctgcc tgaaggcggc ggcatcaatt aaaaagtcgg
    10021 cgatttcgcg gcgcgcttcg ccgtccagcc gctgccattc gcgccggcgc atggctgtca
    10081 ggcggtcaag tgtgctgcgt tcgggcaaca tggtggcgag gttttcccac aggcgcagtt
    10141 cgccttcaaa atcaaaggcg acggtgtcga accctgcgaa aacgtgcagg tttctcctcg
    10201 ccagcatggt tgtccacgat tcgggaagct gtccgccggt aaagttgaac acgggcataa
    10261 ccggtttggc acaccatgaa aggatggtca actcgtccct gtatttgtcg aggacgggtt
    10321 cgcgcgcgtc gatgacgtac attgccatat cgctttgcaa gacttgccgt aagactttgg
    10381 cttcctgatt gaaatcatgg tgcgcaccgt ggctgccgag aaactgttgc agccgttcga
    10441 tgccgtctga acgattgtcc gtatggtttt ccagccattc cagcacgccg cccgcgtctt
    10501 cgagtccggg cgtgtcgtac aggaaaacca gcgtgtctgc gccgtcgctg atggcggctt
    10561 cttcgacatg acgcgtggtc gatggggcgt ttttgacttc gccgaaaccg ctgtcgcgca
    10621 aaagggtacg caggagcgag gttttgccgg tgttggtgtg tccgacgacg gcgagggaaa
    10681 ggggttgttt gttcatgatg tttttgaaga atggattttc agacggtctt ttttcagaat
    10741 ggcggcttaa cagaacattt caagtgagtt tattggtctt tcaaacgccc ttcctgcgcc
    10801 gccctgtcag gctcaagcca cgccgcgccg cattcggcca gcgcgttacg ccaatgttcc
    10861 agcttttccg aaaggtcgtc tgaaagcccc tgttccgcca aaagctgcac caccgcgccg
    10921 ccctgcgccg cttccgagag tcggacaatc tgccgcaaca cgccgcggtc cggcacagtt
    10981 tgggcgcgca cgccgataag cagttgcgcc ggtttctgct tcagctctgt ctccagcgcg
    11041 gcaacctgtt cccgattggt ggcaacgccc ttatccagcc attcctgcgc cagcctgccc
    11101 tcgaaccatt cgccgtcctg ccactcggtc tccagcatga ccgcccattt cggcgcatcg
    11161 ttcaagatga ttttcggtga aacggcggac acggtttccc gacgcgtatc cgcatcggtg
    11221 attttgttct gccagcggcg gatgaccgcc tgataatagg gcttttccaa atccaatccg
    11281 ttttcgcttg ttttcaaaag gattttacac actacccaag ccagcaggcg cggcaggatg
    11341 ccgtagcagg cgatactgcc gaccagcagc cccgaccaag cccgcgcatc ggcaatattg
    11401 ccgttcagac ggccttcgat gaccgcccgc gcatcgggga cagggaaacc gagtttcgac
    11461 ggcagccatg ccaacatttc caccgcgcgt accgaagcgg cattgctcaa cagcgtgctt
    11521 tcccagttga acgtatattg ccgcaccaaa agcagcaaca ataccgacac cagcattccg
    11581 agcagcgtgc agagccacag gctgtgcgac gttgcgccta ttttccaacg taccgaaggt
    11641 tgccgccact cgtccgcata cagccgcaac accgcctgat ttacagggtc tttgccccga
    11701 aaccacgtcg ccggactgct gaaaaaacgc cccactttca cacgcaggaa caacattgcc
    11761 aaccatactg ccagcatcag cgtattcatg cccaacacgc ccgccaaaac caaaaagaaa
    11821 ttcagaccct gattgtccat tagaagataa gtgactgaaa aaccggtaaa aaatgcaaac
    11881 gtcgccgcca ccacccacaa ccagaacgac cccgcacgca cacgttccaa cgtctcccgc
    11941 agcatacggt tcctgtcaat catctccgcc cgacggatga ttttttcctc cgtactgccg
    12001 tccacgcggc gcaaagcctc cgtcgcctgt acgggatcgc cgctgaaaat aaaaccgcct
    12061 tcgtccaaaa tacggaccag ctcaaccagt tttcgggatg gattcaacat aaaatgccgt
    12121 ctgaaaataa aaaacagatt ttaacacacg cattttcaag aatattcaca gtgtaggcaa
    12181 agagtaaatc tcacacagag gcaaaagtat cggcgtaaac tgactgcctc tactttcccg
    12241 aaagattgtg cgatgtatac aggcgaacgc ttcaatactt acagccattt gagcggtttg
    12301 attctggcgg cggcaggttt ggcgctgatg ctgctgaaaa ccataggaca cggggacggc
    12361 taccgtatct tcagcgtatc ggtttacggc atcagccttc ttctgctcta tttgagttcc
    12421 tcgctgtacc acggaattgc agccggaaaa ctgaaaagca ttttgaaaaa aaccgaccac
    12481 tgcatgattt atgtgctgat tgccggaagc tacacaccgt ttgcactggt ttctttgaga
    12541 aacgggccgg gctggacggt attttcactg tcctggctgc tggcggctgc aggaatcgca
    12601 caagaactca ccattggacg gaaaagcgaa aaacgactgc tgtctattgc gatttatatc
    12661 gtaatgggct ggatggtctt ggcggtaatg aaatccctga cagcctcact cccgccggca
    12721 ggactggctt ggctggcggc aggcggtatg ctgtacagcg tcggcattta ctggtttgta
    12781 aatgatgaaa aaatccgaca cgggcacgga atctggcatc tgttcgtatt gggcggcagc
    12841 atcacccaat ttgtcagcgt gtacggttac gtaatctaaa tgccgtctga aaagcaaaac
    12901 ctcccgttcc tgaagattgg gaggttttct gtttgccgga catcagccct tgtcgtggaa
    12961 ctcgtggaat tcatacggat aagacaaatc ccgacccgct tttttttgtg ccaaataatc
    13021 atcataaatg gcacggattt ccttacgcaa caaaaacagg gctatcaagt tcgggataac
    13081 cataaagccg ttgaacatat cggacaggct ccaaaccaaa tcgactttgc cgagcgtacc
    13141 caaaacgatg gcaagcagaa ccaatgcgcg atagatgccc aagtgtcttc ccctgaaaag
    13201 aaaacggatg ttggactcgc cgaaataata ccagccgata atggtcgtca aggcgaaaaa
    13261 ggtcagacac acggcaagca attgcgaacc gaagcccgga aatgccttgt taaaggcaaa
    13321 ttgagtaacc gccgcgccct gttcgcccga aaggttggca tcggtcagca ggataatcag
    13381 cgcagttgcc gtacatacca aaatcgtatc gataaacaca ccgacaaatg ccgccatacc
    13441 ttgctgcacc ggatgcttca catccgcagt cgcgtgggcg tgcggggtag aacccatacc
    13501 cgcttcgttg gaaaacagac cgcgcgccac gccgaaacgg acggcttcgc gcataccgat
    13561 acccgccgca ccgcccaaaa cggcttcggg attgaaggcg gcggtaaaga tgtggttgaa
    13621 catcggcaca atatggtcgg aaaattcaaa caggataacg acggcgcaca aaatataaac
    13681 aaccgccata aacggcacga caaattgggc aatattggca atacggttca cgccgccaat
    13741 cacaaccatg cccgcaagga cggcaagcac aataccgact gccaaagaag gtacatcaaa
    13801 tgcaatggta acggcagaag cgatggagtt tgcctgagtc gcattgccga taaagcccaa
    13861 tgcgacaatc agcgcgatag agaaaaagcc cgacagaaaa cgcgccgcgc ccctgccgat
    13921 tttcggagtc agaccgtgcg taatataaaa cgccggcccg ccgatgtatt tgccgtggct
    13981 gacgacgcgg tatttctgcg ccagcagcgc ttcggcaaaa atcgtggaca tccccaaaac
    14041 ggcagaaagc cacatccaaa aaatcgcgcc cggcccgcct gcggtgatgg cggtcgccac
    14101 gccggcgacg ttgcccgtac cgatttgcgc agatatggca accgccaacg cctgaaactg
    14161 cgataaagac ttgtcgtctt tatcgccttt ggcaaacaag ccgccgaata cggatttgaa
    14221 tcccgcgccc agcttggtaa tctgcggcgc accgagatac agcgtaaaaa acaggccgat
    14281 acccaaaagc gcgtaaatca gcaggtagtc ccaaaggaac cgattgactg tacccaccag
    14341 aacagacaat atattttcca taaaataaac cttatcttac aattaaaatg actgccttcc
    14401 aaaagacatt ccaataagga aacacggcga gcagaccgta tttgccgcaa cagatgcctt
    14461 aaattgtcaa caatcgggga gaagctgcgc catcataccg taaaatatcg ttaattaaaa
    14521 catttcttta tttttaagcg gaaagcggag gaaatcgctt tcagacggca tcgacaacgg
    14581 cacggcataa aacaggatat tttgggtact tgcaacttat gttaaaatgc cgaccgtaaa
    14641 aaatctgaca aaaacagatt aattatttga aataagaaag gaaatttatg gcaggccata
    14701 gcaagtgggc gaatatccag cataaaaaag cccgtcagga tgccaaacgc ggcaaaatct
    14761 tcacccgttt aatcaaagaa atcaccgttg cggcgcgtat gggcggcggc gaccccggtt
    14821 caaatccgcg cctgcgcctg gctttggaaa aagcagccga aaacaatatg cccaaagaca
    14881 atgtgcaacg cgccatcgac aaaggtacgg gcaacttgga aggcgtggaa tacatcgagt
    14941 tgcgctacga aggctacggc atcggcggcg cggctttgat ggtggactgc ctgaccgaca
    15001 acaaaacccg caccgttgcg gacgtacgcc acgcgtttac caaaaacggc ggcaacttgg
    15061 gtaccgacgg ctgcgtggcg ttcaacttcg ttcatcaagg ctatttggtg tttgaaccgg
    15121 gcgttgacga agacgcgctg atggaagcgg ctttggaagc cggtgcggaa gacgtggtta
    15181 ccaacgacga cggttccatc gaagtcatta ccgcgccaaa cgattgggcg ggcgtaaaat
    15241 ccgctttgga ggcggcaggt tacaaatccg ttgacggcga cgttacgatg cgcgcccaaa
    15301 acgaaaccga actctccggc gacgatgccg tcaaaatgca aaaactgatt gacgcgctgg
    15361 aagatttgga cgacgtgcaa gacgtttaca cttccgccgt attgaatctg gactgatacg
    15421 cagcacagca gacatacaat aaaatgccgt ctgaaccttt cagacggcat tgatttattt
    15481 agcccttgcc cacgcccacc aaaccgtcag gacaaccgcc agaaaatacg ccacactcca
    15541 aacaggcaaa gcaaccccca gcagataatc cggttcggca caatttccga acccgcgcac
    15601 gacaggctcg aaccaatcaa acaaaggcca gcccttcaat cggaacgtcc acggcgcgcc
    15661 gcacgaagga gcggttcccg gcggcagcga ctgcaaccac aactgatatg ccgcaacaga
    15721 aatacccgta acggcgggaa tgctgataaa gacagcaccg aacaaaccgc ctgcctttct
    15781 tcttggtctg cacatcagga caattgccgc acacaatgcg gttgccaaga cgcacaaccg
    15841 ctggctgata cacaaaacgc aaggctccat acccaaaaca tactgtgccg ccaaagagcc
    15901 ggcaaatgca cagaccgaaa cggcaaacag cagccaaacg gcttttctaa ataacggggt
    15961 cattttctca acacaccaat caaaataccg atatgccgat tttgctggat atatcccgag
    16021 atggcaaggg acaaaacggc gatttgcccg cacaatgccc acaaaaaaat acatccggag
    16081 gatttgaatt tcagcaaatt tagcgaatca aaagtttatt tcaatgaaat catatgattt
    16141 ttttgaataa gcggattgat gggtttttga aggaatttgt taccggatag ccatcgggca
    16201 agttttttgc aaaatttgaa tcggtcgggt aaattttcaa aaaataattg acagcggata
    16261 agaaacggcg gataattccc gccgtcgagt tgcttgatgc agcttgattt ttctcctcta
    16321 tttctccttt gtagacttgg cacacattca actggatgtg tgcatttttt tatctccgct
    16381 ttttttgaaa tttaattact tttattgttt gaaattccat tctttaagaa atttaacaag
    16441 aaaaacgctt gctttttgac cggaaaagct gcataattca ttccgttagc tggttcgagt
    16501 agtcagttaa tagtttctcc tctatttctc ctttgtagac ttggcacaca ttcaactgga
    16561 tgtgtgcatt tttttatctg aagcaacaag cctctgtgcg tgatgttgtt atgtttcatt
    16621 taggtgtcaa accgcatatc cggtctgaaa tattcaatcc aaatccaaaa ccggattttc
    16681 tttgacctcc tccatcacaa cataactcct actctccgaa gcggcaggca gttgcaatag
    16741 gatattgcct agcatatccc gataggcaga catatcgggc aaacgtactt taatcagata
    16801 gtcgtattcg cccgacacca agtggcattc cataatttgc ggaattttca gcacttcttt
    16861 tttgaaatct tcgaaaatat tgcccgattt ggattgcagc ttcagctcga caaaaaccaa
    16921 taaaggtttg cccaacagat ggggattgag atgggcgtga taaccggaaa tataatgttc
    16981 ccgctccaaa cggcgcaccc tctctgtaac gggcgtggtg gacaagccta ccttctcggc
    17041 aagctccgtc atcgggatgc gggcattctg ttgaaggatc ttaaggatgc ggaaatcgat
    17101 tttatctagt tctttcattt agattgcctt gtatttatta ttgattttaa caaataga
//
LOCUS       DAWGRZ010000024        17026 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000024 DAWGRZ010000000
VERSION     DAWGRZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17026
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.024"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..518
                     /locus_tag="WHT44_000559"
     CDS             <1..518
                     /locus_tag="WHT44_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ2532780.1"
                     /translation="NRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNADELI
                     GNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVARSE
                     LQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR
                     VKKNDPSRFLN"
     gene            537..755
                     /locus_tag="WHT44_000560"
     CDS             537..755
                     /locus_tag="WHT44_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532781.1"
                     /translation="MSMPEIPKWYDDDGQIVSCTEKIKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            900..1244
                     /locus_tag="WHT44_000561"
     CDS             900..1244
                     /locus_tag="WHT44_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ2532782.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            1244..1936
                     /locus_tag="WHT44_000562"
     CDS             1244..1936
                     /locus_tag="WHT44_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ2532783.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            2003..3223
                     /gene="argJ"
                     /locus_tag="WHT44_000563"
     CDS             2003..3223
                     /gene="argJ"
                     /locus_tag="WHT44_000563"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ2532784.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFNEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            3283..4692
                     /locus_tag="WHT44_000564"
     CDS             3283..4692
                     /locus_tag="WHT44_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ2532785.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGVPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            4882..6273
                     /gene="hrpA"
                     /locus_tag="WHT44_000565"
     CDS             4882..6273
                     /gene="hrpA"
                     /locus_tag="WHT44_000565"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ2532786.1"
                     /translation="MFPDFSQTLSKDRHFLRSAFKNPNKYGGLSKIEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLEEIKKAIAKNQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIRETAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMAALKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAV"
     gene            6417..8060
                     /locus_tag="WHT44_000566"
     CDS             6417..8060
                     /locus_tag="WHT44_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ2532787.1"
                     /translation="MDEIQIPKKVELQTKLENEKIVLSKGSTTIIVGANGTGKTRLAV
                     YIEEQLKEKAHRISAHRALKLNPNVNKIPEKSAKTYLSYGQNWDGIDVSNRKNYRWDN
                     NSYTHLLNDFDWLLQYLFAQQNNIAVANNQKLNRNEKVTNSKTKLDILQEAWETLLPH
                     RKLHITADDIQVSAVDNEELYSASNMSDGERALFYILGQVLSVDDGSVLIFDEPELHI
                     HKSIISNLWDKIEELRPDCSFLIITHDIEFAATRVAKKYVIRNYYPTPAWDISEVPES
                     NFDEETITMILGSRKPILFVEGNNNSLDIATYRYCYPDWTIIPKGACKDVIQSVSSLK
                     KLSNEMPLLNLKCSGIVDLDSRDEREIEQLNNLGIYILPVSEIENLFSLTDVAKEILK
                     LNQYSDEELLNKLNGFKSELIKYIDNELKDDKLDEFVVKQVRRKIDNYLKNIDLSSKI
                     TSTDMKKSLLNEISTLTEQKIETWISEIKNEIQRCIEQQDLDKLLTIYDNKGLLAKSA
                     CVLKGMRNKHEFESWIMRTLKGRNKDFIDAIRQKLPILD"
     gene            8104..8286
                     /locus_tag="WHT44_000567"
     CDS             8104..8286
                     /locus_tag="WHT44_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532788.1"
                     /translation="MKNHQTTLSFPTSSSLKPCADKQGLSEDRSQYRLTKLGEQMAHL
                     PIDPKIARILLVLFRF"
     gene            8498..10075
                     /gene="lpt3"
                     /locus_tag="WHT44_000568"
     CDS             8498..10075
                     /gene="lpt3"
                     /locus_tag="WHT44_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HEZ2532789.1"
                     /translation="MMKKSFLTLVLYSSLLTASEIAYRFVFGIETLPAAKIAETFALT
                     FVIAALYLFARYKVTRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPVLWGVLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSRIPAFKQPAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNAIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENEMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGKHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRDGKAEYVYPQ"
     gene            10177..13560
                     /locus_tag="WHT44_000569"
     CDS             10177..13560
                     /locus_tag="WHT44_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEZ2532790.1"
                     /translation="MDARSNPANVSDGLQNSSGHIGTNTRYRLTKLGEQIARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDASAKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRPPEVRQLTSSENAGDQDLSAKLKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTRLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRPVSYGKVAPEEAR
                     EIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYNERLP
                     EMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSRQNENHGNTVGWV
                     ENPTSAATAKTVGFDNPTYAAQQTTPSPVGEGRGEGKTVAAQTNFSATAANPLPNPLP
                     QEREQSAAASTISDDLRPANLQQTAPSPVGEGWGEGKTVATQTNFSATSTNPLPQERE
                     QSASASTFSDDLRPANLQQPSPSPVGEGWGEGKTVATQTNFSATSTLSDDSKPKKQPA
                     PQKNRLKPLPLADIRTFQAWLKTAERDNPRLLFLSRDDLMQHAAAHITEEQFPKFWQT
                     ADGKFKLSYRFEPHHPLDGVTMTVPLTVLNRLHAPSLEWLVPGMIREKIQLQIKALPK
                     QIRRICVPVPEFITQFLSQNPDRNAPILPQLAQAIAKTAGDIRIFEQINQDEWAAFRL
                     PEHCYFNLRIIDDGGQELAGGRKLHELQQQLGQAAAVTFRDNTQEFERDNVTAWDIGT
                     LPESIKFARGKQQLTGYLGLQKEKDGRIALRLFDTTEAAEQAHRQGVIELMKLQLKEQ
                     VKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDELPRNEKAFKEQIKR
                     ARSRLPAVKEALSRYLQETAAVYAELNSKLGKHPLTHLLRLRLQTLLAAGFATRTPWA
                     QWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKTDSLIKQGLPISDGL
                     AAFKWMIEELRVSLFAQELKTPYPVSVKRLLKEWEKIEK"
     gene            complement(13593..14684)
                     /locus_tag="WHT44_000570"
     CDS             complement(13593..14684)
                     /locus_tag="WHT44_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEZ2532791.1"
                     /translation="MQTFNRERLEFALDKRGCTQTKLAELLGITARQVRNYVKDEQIP
                     DLNELANLLEFPVHFFTNDERLPELKSQAVSFRARSRTSKRLEKQALNHSITAFLLND
                     WLETEFTLSQAVLPDFSDISPEEAAQELRLEWGLGNEPIPNMITLLEVKGVRVFSLSL
                     DTKDIDAFCTWYENHPFVFLNTQKSAERSRFDAAHELGHLIRDKYSMEHSKNSEITED
                     EPRDIIEKEANAFASAFLMPEAALRLYRHVPITIENLLKIKRRFGVSLVALAYRMHKL
                     GMITDRMYIHNLCPLFAKKKYRTIEPEPMEREISSALRKMLTILKNDGIGIEQIAENL
                     NVSPKDVADLTFGLVERDLNRYRKLRLVK"
     gene            complement(14700..15344)
                     /locus_tag="WHT44_000571"
     CDS             complement(14700..15344)
                     /locus_tag="WHT44_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532792.1"
                     /translation="MVEFALSEPFPFEVGEIVEQQNSDIALSEISKYLSQEMLKEIVL
                     TAHNNIQDKAALSDRNTLGMYRQAEITFALRVYLSKDGWTLEPWSGGAFLCLSPCRTK
                     SIIVATATSDVGRHHGMPMTTARKGKGFEEAIRNSKLDLPDTVQFWVLLFHWKYNKVQ
                     LELSLPSGFVSHQITGYKQRIILADVDLTKFDELERKQPVEEYEPLVERKQKAG"
     gene            15701..15853
                     /locus_tag="WHT44_000572"
     CDS             15701..15853
                     /locus_tag="WHT44_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532793.1"
                     /translation="MSGIYLPRLFPPHIAERGLLYFQQGKVLDVRKTSAGHYRAEVCG
                     SENYWV"
     gene            16223..16378
                     /locus_tag="WHT44_000573"
     CDS             16223..16378
                     /locus_tag="WHT44_000573"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ2532794.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(16468..16917)
                     /locus_tag="WHT44_000574"
     CDS             complement(16468..16917)
                     /locus_tag="WHT44_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ2532795.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         4622 a         4626 c         3808 g         3970 t
ORIGIN      
        1 tcaaccgagt ccccgcccgg cgggcgggca atgccgacga actcatcggc aacgcgatgg
       61 ggcttaacga acagcccgtt ttacccgtca accgagcccc cgcccggcgg gcgggcaatg
      121 ccgacgaact catcggcaac gcgatgggac ttttgggtat tgcctaccgc tacggcggca
      181 catcggtttc taccggtttt gactgcagcg gcttcatgca gcacatcttt aaacgcgcca
      241 tgggcatcaa cctgccgcgc acgtcggcag aacaggcacg gatgggtacg ccggttgccc
      301 gaagcgaatt gcagcccgga gatatggtgt ttttccgcac gctcggcggc agccgcattt
      361 cccatgtcgg actttatatc ggcaacaacc gcttcatcca cgcgccgcgc acggggaaaa
      421 atatcgaaat caccagcctg agccacaaat attggagcgg caaatacgcg ttcgcccgcc
      481 gggtcaagaa aaacgacccg tcccgctttc tgaactgatt tcccaaggaa tacgcaatga
      541 gtatgcccga aatacccaaa tggtacgacg atgacggaca gatcgtgtcc tgtaccgaaa
      601 agatcaaagt gatgtccgaa aatatggccg agctgtatca gacggcacaa gacgcgtttg
      661 aagacgcgct gctgatgggt tgcggcgaac gtcagttgcg cgattacctg ctcgcgctga
      721 ttgaaggttt ggaaaatccc taccgcaaag tctgaacacg ccccggttgc tgcggcacgg
      781 tttatccgtg ccgtttttgc gtttgtgcgc ggcttcggct tttcagacgg catatttgac
      841 gttatgatta aacagttaac aagatttatc acaacgccgt caaaagacag acacacaaca
      901 tgaacatcat ccgcgcgctc ctcatcatcc tcggctgcct cgccgccggc gaaaccgccg
      961 ttttcctagc aggcatcaaa ctgcccggca gcatcgtcgg catgggcgtg ctgtttgcgc
     1021 ttttgcaggc aggttgggtc aaaacgtctt ggctacaaca gcttaccgac gcgctgatgg
     1081 cgaatctgac gctgtttctc gtgccgccct gcgtggcggt catcagctat ttggatttga
     1141 ttgccgacga ttggttttcg atactggttt ccgcctccgc cagcactttg tgcgtactgc
     1201 tggttacagg caaggttcac cgctggatac ggagcattat ctgatgaacg aaatcctcag
     1261 gcagcccagc gttctgcttt tcctcacgct tgccgtgtac gcgcttgcga ttatcgtgcg
     1321 cacgcgcacg ggcaatatct tctgcaaccc cgtactcgtc agcactatcg tgctgattgc
     1381 ctacctgaaa atcctcgaca tcgattatgc ggtgtaccac aacgccgcgc aattcattga
     1441 tttttggcta aaacccgccg tcgtcgtgct tgccgtgccg ctctaccaaa accgccgtaa
     1501 aatcttcaac cagtggctgc ccgtcatcgt ttcacagctt gcgggcagcg ttacgggcat
     1561 tgttacgggg atgtattttg ccaaatggct gggcgcggaa cgcgaagtcg tcctctcgct
     1621 cgcgtccaaa tctgttacca accccatcgc cattgaaatc acccgctcca tcggcggcat
     1681 tcccgccatt accgccgcca ccgtcatcat tgccggtctg gtcggacaga ttgccggtta
     1741 caaaatgctg aagaacacag tcgtcatgcc ctcgtccgtg ggtatgtcgc tcggcacggc
     1801 ttcgcacgcg atggggattg ccgcctcgct cgaacgcagc cgccgtatgg cggcatacgc
     1861 ggggctgggg ctgacgttca acggcgtact gaccgcgctg attgcgccgc tgctcatccc
     1921 cgttttggga ttttgaaccc gtttcagacg gcatttcagc ccatgctgtc tgaacgccga
     1981 cacactcgca aggagaaccg ttatggctgt caacctgacc gaaaaaaccg ccgaacaact
     2041 gcccgacatc gacggcattg ccctctacac cgcccaagca ggcgtgaaga agcccgggca
     2101 taccgacctg acactgattg ccgtagccgc cggcagcacc gtcggtgcag tcttcacgac
     2161 caaccgtttc tgtgccgcgc ccgtccacat cgccaaatcg caccttttca acgaagacgg
     2221 cgtgcgcgcc ctcatcatca acacgggcaa cgccaacgcg ggtacgggcg cacagggcag
     2281 aatcgatgct ttggcagtgt gtgccgccgc cgcccggcaa atcggctgca aaccgaacca
     2341 ggtgctgccc ttctccaccg gcgtgattct cgaaccgctg cccgcagaca aaatcatcgc
     2401 cgcgctgccc aaaatgcagc ctgccttctg gaacgaagcg gcacgcgcca tcatgaccac
     2461 cgacaccgtg cccaaagccg cctcgcgcga aggcaaggtc ggcgacaaac acaccgtccg
     2521 cgccacgggc atcgccaaag gctcgggcat gattcatccc aatatggcga ccatgctcgg
     2581 tttcatcgcc accgatgcca aagtttccca acccgtcctc caactgatga cgcaggaaat
     2641 cgccgacgaa accttcaaca ccatcaccgt tgacggcgac accagcacca acgacagctt
     2701 cgtcatcatc gccaccggca aaaacagcca aagcgaaatc gacaacatcg ccgacccgcg
     2761 ttacgcccaa ctcaaagaat tgttgtgcag cctcgcgctc gaactcgccc aagccatcgt
     2821 ccgcgacggc gaaggtgcga ccaagttcat caccgtccgc gtcgaaaacg ccaaaacccg
     2881 cgacgaagcc cgccaagccg cctacgccgt ggcacgttcg ccgctggtca aaaccgcctt
     2941 tttcgcctcc gaccccaacc tcggcaggct gctcgccgcc atcggttatg ccggcgttgc
     3001 cgacctcgat accgacctcg tggaaatgta tctcgacgat attttggttg ccgaacacgg
     3061 cggacgcgcc gcaagctaca ccgaagcaca agggcaggcg gtgatgtcga aggccgaaat
     3121 caccgtccgc atcaagctgc atcgcggaca agccgccgcc accgtctata cctgcgacct
     3181 gtcgcacgga tacgtttcca tcaacgccga ttaccgttcc tgacccgaca cggcttcaga
     3241 cggcatacat aaaatgccgt ctgaaccgcc ggacaacata ccatgacctc cacattcccc
     3301 cgccgcctcg cccgcaaaat ccgccaaacc cgccgcctgt cgcgcaaaag catcgccttt
     3361 ctgttccttt tggcaggttc ggcactcgtc gccctgaccg cgctgttttt tgcccatctt
     3421 gccgattttg cgctggaact gaacgccaaa ctggttcaac aatacccgtg gttcgcgtgg
     3481 gtcgcgcttc ctttgggttt accgcttatt gcgtggctca cacgcaaatt cgcccccttc
     3541 accgccggca gcggcatccc gcaggtcatc gcctcactgt cgctgcccta cggcgcacag
     3601 aaaacgcggc tgatccgcct cgggcagacg ctgctgaaga ttccgctaac ctttttgggt
     3661 atgctgttcg gcgcgtccat cggacgcgaa ggtccgtccg tgcaggtcgg cgcggcagtg
     3721 atgggcgcgt ggggcgcgtg gtgcaagaaa cacggcttgg cattcaaagg gatgcaggaa
     3781 aacgatttga tggcggcggg cgcggcgggc ggtttggcag ccgcgttcaa cgcgccgctg
     3841 gcgggcgtga ttttcgccat tgaggaactc gggcgcggca tcatgttgcg ctgggagagg
     3901 caaattcttt tgggcgtgct cgcctccggt ttcatacagg tcgccattca gggcaacaac
     3961 ccgtattttt ccggcttcaa cggcggcgta ttggaacata tctttctgtg ggtcgcactg
     4021 tccggcctgg tttgcggcgc ggcgggcggg ctgttcggac gtttgctcta tcgcggtgcg
     4081 gcggcgtttg caccgcgcaa gatacgcggc ttcatccgca accgtccgct gctgctggcg
     4141 gcactgatgg ggctgctgct cgccctgctc ggcacgttct accaaggcaa aacctacggc
     4201 accggctacc acgaagccgc ccaagccctg cacggcatct acgaagcccc cttcggactc
     4261 gccgccgcca aatggctcgc caccgtattc agctattggg caggcgttcc gggcggcatt
     4321 ttcactccct cgctgaccat aggcgcggtt ttgggcgagc atatcgccgc catcgccgac
     4381 atatcgcagg gtgcaaacat catcgtcctc atctgcatgg cggcatttct ggcgggcgcg
     4441 acacaatccc cgattacttc cgccgtcgtc gtcatggaaa tgacgggcgg acaaagcctg
     4501 ctgttttgga tgctaattgc ctgcattttc gcctcgcagg tttcgcgcca gttttcgccg
     4561 cgtccgttct accacgcatc gggaatgcgc ttccgccagc gcgtgcttca agaaaccgcc
     4621 gcccaaaccg gcaatgcgcc cgcaagaccg caaacagcaa acagcaaaac gggaatgccg
     4681 tctgaaaatt aaaacgcccc cgatcaaacg ccggcagccg ccttgatttg aataccgttc
     4741 cgccgccgct tgaaatttca gcaacaatgc cgtctgaacg acagaatgcg gttttcagac
     4801 ggcatttccc catcccgata ttgcctaaac aaaaccgaag cgtttgctat aattctattt
     4861 tttaccgcat acgcaccaat catgtttccc gatttctctc aaaccctctc caaagaccgc
     4921 cacttcctgc gttccgcctt caaaaatccc aacaaatacg gcggtttgtc caaaatcgaa
     4981 gaaaaatacc gaaaatcgca cgaaatcttt ttgaagcgtt tggcagcctt gccaaaaccc
     5041 gaattcgaca acaccctgcc cgttcacgag aagctcgaag aaatcaaaaa agccattgcc
     5101 aagaatcagg taacgattat ttgcggcgaa accggttcgg gcaaaaccac gcagttgccc
     5161 aagatttgct tggaactcgg gcgtggggcg gcaggattga tcgggcatac ccagccgcgc
     5221 cgtttggccg cgcgctccgt agcagagcgg attgccgaag agctgaaatc cgaaatcggc
     5281 agcgcggtcg gctataaagt acgcttcacc gaccacacct cgcgcgatgc ctgcgtcaag
     5341 ctgatgaccg acggcatcct gctggcggaa acgcagaccg accgttatct cgccgcctac
     5401 gacacgatta tcatcgacga agcgcacgag cgcagcctga acatcgactt ccttttgggc
     5461 tatttgaaac aactcctgcc gcgccgcccc gatttgaaag tcatcatcac ctcggcaacg
     5521 atagacgcag aacgcttctc ccgacacttc aacggcgcgc ccgttttaga agtgagcgga
     5581 cggacgtatc ccgtcgaaat cctctaccga ccgctgaccg gcaaagacga agacgacgca
     5641 gaagtggagt tgaccgacgc gattgtcgat gcggcggacg aattagcgcg acacggcgaa
     5701 ggcgatattt tggtattcct gccgggcgag cgcgaaatcc gcgaaactgc cgaagccctg
     5761 cgcaaatcca cgctgcgccg caacgacgaa atcctgcccc tgttcgcacg cctgtcgcac
     5821 gccgagcagc acaaaatctt ccacccctca ggcgcgaaac gccgcatcgt attggcaacc
     5881 aacgtcgccg aaacctcgct taccgtgccg ggcatcaaat acgtcatcga caccggcctc
     5941 gcgcgtgtta aacgctattc cgcacgggcg aaagtggagc agcttcatat cgaaaaaatc
     6001 tcccaagccg ccgcccgcca acgatccggc cgctgcggac gcgtctccgc aggcgtgtgt
     6061 atccgactgt tttcagaaga agattttaac agccgccccg aatttaccga ccccgaaatc
     6121 gtccgcagca acctcgccgc cgtcatcctg cgcatggcag cattgaaact cggcgatgtg
     6181 gcggcattcc cgtttttaga aatgcccgat tcacggtata tcaatgacgg ttttcaggtg
     6241 ttgttggagt tgggggcggt ggaggccgtc tgaaaacagg cagacataaa agaaaatccg
     6301 cgtagagtga tgtaaactta cccttgcttt aataagtaga aaatggtggg tttacgtccc
     6361 cccctgcggc tactaaaaaa atataagagt aaacaacctt tttgaaagaa aaatgtatgg
     6421 acgaaattca aatacccaaa aaagtggaat tacaaaccaa actagaaaat gaaaagattg
     6481 ttttatcgaa aggttctacc acgattattg ttggtgctaa tggcacaggg aaaacaagat
     6541 tagctgttta tattgaagaa caattaaagg aaaaagcaca cagaatttcg gctcatagag
     6601 cattaaaatt aaaccctaat gtcaataaaa taccagaaaa gagtgccaaa acatatctat
     6661 cttatggtca gaactgggat ggaatcgatg tatcaaatag aaaaaattat agatgggata
     6721 ataactcata tactcattta ctcaacgatt ttgattggtt attacaatat ttattcgctc
     6781 aacaaaataa tattgcggta gcaaataatc aaaagctcaa ccgtaatgaa aaagtaacca
     6841 attcaaaaac aaagctagat attttgcaag aagcatggga aacattatta ccacacagaa
     6901 aattacatat tacagcagat gatattcaag tctctgctgt agataatgag gaattgtatt
     6961 ctgcctcaaa tatgagtgat ggagagcgag cacttttcta tattcttgga caagttttgt
     7021 cagtagatga cggttctgtc ttaatttttg atgagcctga attacatatt cataaatcaa
     7081 ttatttcaaa tctatgggat aaaattgaag aattacgacc tgattgttca tttctaatca
     7141 ttacacacga tattgaattt gctgcaactc gagtagctaa aaaatatgtt atcagaaatt
     7201 attatccgac ccctgcttgg gatatttctg aagttcctga aagtaatttt gatgaagaaa
     7261 caataacgat gattttaggt agccgtaagc caatattatt tgttgagggc aacaataata
     7321 gtttagatat tgctacttac cgctattgtt atcctgattg gaccatcata cccaaagggg
     7381 catgcaaaga tgtcattcaa tcagtatcat cgctgaaaaa attaagtaat gaaatgccat
     7441 tactaaactt aaaatgttca ggtattgtcg atttagatag tagggatgaa agagaaattg
     7501 aacaattaaa taatttgggt atttacattt tacctgtatc cgaaattgaa aatcttttta
     7561 gcttaactga tgtagcaaaa gagatattga aactaaatca atattcagat gaagaattac
     7621 tcaataaact taatggattt aaatccgaac taattaaata tatagataat gaattaaaag
     7681 acgataaatt agacgaattt gttgtaaaac aggttcgacg taaaattgat aattatttaa
     7741 aaaatattga tttatcctcc aaaataacaa gtactgatat gaaaaaatca ttacttaatg
     7801 aaatttctac tttaacagaa cagaaaattg aaacatggat ttcagaaatt aaaaatgaaa
     7861 ttcaaagatg tattgaacag caagatttgg ataaattact tactatatat gataataaag
     7921 gactcttggc taaatcagct tgtgttttaa aaggaatgcg taacaaacat gaatttgaaa
     7981 gctggataat gagaacatta aaaggaagga ataaagattt tattgatgca atcagacaga
     8041 aacttccaat tctggattaa ataaaaccat ctgaaaattt accttcagat acagatatat
     8101 ttcatgaaaa atcatcaaac tacactctct ttccctactt cgagtagcct gaaaccttgc
     8161 gcagacaaac aaggcctgtc tgaagaccgc agccaatacc gcctgaccaa actcggcgaa
     8221 caaatggcgc acctgcctat cgacccgaaa attgcgcgta ttttgttagt attattccgt
     8281 ttttaaaaat gcccgattcg cggtatatca atgacggttt tcaggtattg ctggaattgg
     8341 gggcggtgga ggccgtctga aaataaaatc tttctttata aaaaggcagg ccatgtttca
     8401 ttttcagacg gcctaaatca ttgagaaact aaaaactatt aaaaagggaa tattgggttt
     8461 taaaactcaa tcggtaaatt tttattgtga aatattaatg atgaaaaaat ctttccttac
     8521 gcttgttctg tattcgtctt tacttaccgc cagcgaaatt gcctatcgct ttgtatttgg
     8581 gattgaaacc ttaccggcgg caaaaattgc ggaaacgttt gcgctgacat ttgtgattgc
     8641 tgcgctgtat ctgtttgcgc gttataaggt gacgcgtttg ttgattgcgg tgttttttgc
     8701 gttcagcatt attgccaaca atgtgcatta cgcggtttat caaagctgga tgacgggcat
     8761 caattattgg ctgatgctga aagaggttac cgaagtcggc agcgcgggtg cgtcgatgtt
     8821 ggataagttg tggctgcctg tgttgtgggg cgtgttggaa gtcatgttgt tttgcagcct
     8881 tgccaagttc cgccgtaaga cgcatttttc tgccgatata ctgtttgcct tcctaatgct
     8941 gatgattttc gtgcgttcgt tcgacacgaa acaagagcac ggtatttcgc ccaaaccgac
     9001 atacagccgc atcaaagcca attatttcag cttcggttat tttgtcggac gcgtgttgcc
     9061 gtatcagttg tttgatttaa gcaggattcc cgcctttaag cagcctgctc caagcaaaat
     9121 cgggcagggc agtgttcaaa atatcgtcct gattatgggc gaaagcgaaa gcgcggcgca
     9181 tttgaagctg tttggctacg gacgcgaaac ttcgccgttt ttaacccggc tgtcgcaagc
     9241 cgattttaag ccgattgtga aacaaagtta ttccgcaggc tttatgactg cagtgtccct
     9301 gcccagtttt ttcaatgcga taccgcacgc caacggcttg gaacaaatca gcggcggcga
     9361 taccaatatg ttccgcctcg ccaaagagca gggctatgaa acgtattttt acagcgcgca
     9421 ggcggaaaac gagatggcga ttttgaactt aatcggtaag aaatggatag accatctgat
     9481 tcagccgacg caacttggct acggcaacgg cgacaatatg cccgatgaga agctgctgcc
     9541 gttgttcgac aaaatcaatt tgcagcaggg caagcatttt atcgtgttgc accaacgcgg
     9601 ttcgcacgcc ccatacggcg cattgttgca gcctcaagat aaagtattcg gcgaagccga
     9661 tattgtggat aagtacgaca acaccatcca caaaaccgac caaatgattc aaaccgtatt
     9721 cgagcagctg caaaagcagc ctgacggcaa ctggctgttt gcctatacct ccgatcatgg
     9781 ccagtatgtt cgccaagata tctacaatca aggcacggtg cagcccgaca gctatctcgt
     9841 gccgctagtg ttgtacagcc cggataaggc cgtgcaacag gctgccaacc aggcttttgc
     9901 gccttgcgag attgccttcc atcagcagct ttcaacgttc ctgattcaca cgttgggcta
     9961 cgatatgccg gtttcaggtt gtcgcgaagg ctcggtaacg ggcaacctga ttacgggtga
    10021 tgcaggcagc ttgaacattc gcgacggcaa ggcggaatat gtttatccgc aatgagtggc
    10081 gtaaaaacca ataaagacaa atttagatga tgtcggggaa gatgcccgac cgacaagact
    10141 atgcaaaata tgaaaaacca agtacgcgga tcaggcatgg atgcccgatc caatccggcc
    10201 aatgtttcag acggcctgca aaacagttcg ggtcatatcg gtaccaacac gcgttaccgc
    10261 ctgaccaaac tcggcgaaca gatagcgcgc ctacccatcg acccgaaaat cgcgcgcatt
    10321 ttgctggcgg cgaagaaaca cgactgcatg gcggaaatat tggtgattgc gtccgcgctg
    10381 tcgattcaag acccgcgcga gcggccgcta gaagcgcgcg atgcctcagc caaggcgcac
    10441 gagcgtttta ccgacaagca gtccgatttc cttgcctatc tgaacatttg ggacagcttc
    10501 cagcgcgaac gcgataaagg cttgtccaac aagcagctgg tgcagtggtg ccgccaatat
    10561 ttcctgtcgc acctgcggat gcgcgagtgg cgcgagctgc accaccagct tgcccaaacc
    10621 gcgattgaaa tgggtttaac caccaaggaa gccgctttca gacgacctcc cgaagtcagg
    10681 cagctcacgt cgtctgaaaa tgcgggtgac caagacctat ctgctaaact caaacaaaaa
    10741 caactggata aaaagcaaca ccgcgcccaa atccgcgccg ccaaagaagc gggctacgaa
    10801 caaatccacc gcgccctgct cactggcctt atcgccaacg tcggcatgaa atcgcccgac
    10861 ggtaacgact acaccggcgc gcgcggcagc cgcttccacc ttttccccgc ctccgccctg
    10921 ttcaaagcca aacccaaatg ggtgatggcg gcagaattgg ttgaaaccac gcgcctttac
    10981 gcgcgcgacg tcgccgttat ccagcccgaa tggatagagc aggaagcgcc gcacctcgtc
    11041 cgctatcatt atttcgagcc gcattgggaa caaaaacgcg gcgaagtcgt cgccagcgaa
    11101 cgcgtgacgc tttacggtct gaccgtattg ccgcgccgcc ccgtgtctta cggcaaagtt
    11161 gcccccgaag aagcgcgcga aatctttatc cgcagcgcgt tggtggcgca ggaatgcgat
    11221 ttgaaagcgg atttttttgt ccacaacaaa aagctgatta aagaaattac cgaactcgaa
    11281 cacaaatcgc gcaagcaaga cgtgctggtc gatgacgaag ccctgtttgc gttttataac
    11341 gaacgactgc ccgaaatggc ttggaaagac gcgcaaggca gcgtttgggg aagtgaagat
    11401 tccgtacgga ttattgaatc tgacaaagcc gagaggtcgt ctgaaaatga gcgcaacgag
    11461 tttcgtaaaa acaagcgtaa tgggtctcgc caaaatgaaa atcacggcaa caccgtaggt
    11521 tgggttgaaa acccaacatc agccgcaact gcaaaaactg ttgggtttga caatccaacc
    11581 tacgctgccc aacaaaccac cccctccccc gtgggggagg gtcggggaga gggcaaaaca
    11641 gttgccgcac aaaccaactt ttccgcaacc gcagcaaacc ctctccctaa ccctctcccg
    11701 caggagaggg aacagagtgc cgcagcttca acgatttcag acgacctgcg tcctgcaaat
    11761 ctgcagcaaa ccgccccctc ccccgtgggg gagggctggg gagagggcaa aacagttgcc
    11821 acacaaacca acttttccgc aacctcaaca aaccctctcc cgcaggagag ggaacagagt
    11881 gcctcagctt caacgttttc agacgacctg cgtcctgcaa atctgcagca accctctccc
    11941 tcccccgtgg gggagggctg gggagagggc aaaacagttg ccacacaaac caacttttcc
    12001 gcaacctcaa cactttcaga cgactccaaa cccaaaaagc agcctgcacc ccaaaaaaac
    12061 cgtctgaaac ccctacccct cgccgacatc cgcaccttcc aagcctggct caaaaccgcc
    12121 gagcgcgaca atccgcgcct gctgttcctc agccgcgacg atctgatgca acacgccgcc
    12181 gcacacatta ccgaagaaca gttccccaaa ttctggcaaa ccgcagacgg caaattcaaa
    12241 ctttcctacc gcttcgagcc gcaccatccg ctagacggcg tgaccatgac cgtgccgctg
    12301 accgtcctca accgcctgca cgcgccgtcg ctcgaatggc tggtgcccgg catgatacgc
    12361 gaaaaaatcc agttgcaaat caaagcactg cccaagcaaa tccgccgcat ctgcgtgccc
    12421 gtgcccgaat tcatcaccca atttttaagc caaaaccccg accgcaacgc ccccatcctg
    12481 ccccaactcg cccaagccat cgccaaaacc gcaggcgaca tccgcatatt cgagcaaatc
    12541 aaccaagacg aatgggccgc gttcaggctg cccgaacact gctatttcaa cctccgcatt
    12601 atcgacgacg gcggacaaga gcttgccggc ggccgcaaac tgcacgaatt gcaacaacaa
    12661 ctcggtcaag ctgccgccgt taccttccgt gacaacaccc aagaatttga gcgcgacaac
    12721 gtcaccgcat gggacatcgg caccctgccc gaatccatca aattcgcacg cggcaaacaa
    12781 cagctcaccg gctatctcgg cctacaaaaa gaaaaagacg gccgcatcgc cctgcgcctg
    12841 tttgatacca cagaagccgc agagcaggca caccgtcaag gtgtcatcga attgatgaag
    12901 ctgcaattaa aagagcaggt aaaggatttg aacaaaggca tccaaggctt cacccaagct
    12961 gccatgctgc tcaaacacat caacgccgac actctgcgcg acgacctcac ccaagccgtc
    13021 tgcgaccgcg cctttatcgg cgaagacgag ctgccgcgca acgaaaaagc cttcaaagaa
    13081 caaatcaaac gcgcccgcag ccgcctgccc gccgtcaaag aagccctcag ccgctacctg
    13141 caggaaaccg ccgccgtcta cgccgaactc aacagcaaac tcggcaaaca cccattgacc
    13201 caccttctaa gactacgcct gcaaaccctg ctcgccgccg gcttcgccac ccgaaccccg
    13261 tgggcacaat ggccgcgcct ccccatctac ctcaaagcca tgaccctgcg cctcgaaaaa
    13321 tacagcagca accccgcccg cgacgcagcc cgcgaagccg atatccaaga gctggaacaa
    13381 atgtggcagg aaaaaacaga cagcctgatt aaacaaggtc tccccatttc agacggcctc
    13441 gccgcgttta aatggatgat tgaagaattg agggtgtcgc tgttcgcgca ggaattgaag
    13501 acaccgtatc cggtgtcggt gaagcggctg ttgaaagagt gggaaaaaat tgaaaaataa
    13561 aaaaacagcc tgaaaagttt caggctgttt ttttatttga ctaatcgaag tttcctatat
    13621 ctatttaagt ccctctcaac taatccaaaa gttaaatcag caacatcttt gggggatacg
    13681 tttaaatttt cagcaatctg ttcaatacca atgccatcat tttttaaaat agtaagcatt
    13741 ttacgtaatg cgcttgatat ttccctttcc attggctctg gttcgatagt tcgatatttt
    13801 ttctttgcaa acaaaggaca aagattgtgt atatacatcc tatcagtaat cattcctaat
    13861 ttatgcatcc gatatgctaa ggcaacaagt gatacaccaa atcgtctttt gatttttaat
    13921 aaattttcaa tagtgatagg aacatgacga tataagcgta gtgcagcctc cggcattaaa
    13981 aaagctgaag caaaggcatt agcctctttt tcgataatat cacgaggttc atcttctgta
    14041 atttcactat ttttactatg ttccatactg tatttatcac ggattaagtg ccctaattca
    14101 tgggcagcat caaatcgact acgttctgca gatttttgtg tatttaaaaa tacaaatgga
    14161 tgattttcat accaagtaca aaaggcatca atgtcctttg tatctaaaga taatgaaaat
    14221 acacgaacac ccttaacttc aagtagggtg atcatattcg gaataggttc attgccaagc
    14281 ccccattcta atcttagttc ctgagcagcc tcttcaggag aaatatcaga aaaatcaggc
    14341 aatacggctt gacttagtgt aaattctgtc tcgagccagt catttaacaa aaaagccgta
    14401 atgctatgat ttaatgcttg tttttcaagc ctcttcgagg tgcgtgaacg agcacgaaaa
    14461 cttactgcct gagatttcaa ctcaggcagt ctttcgtcat tagtaaagaa atgaactgga
    14521 aactctaata aattggctaa ttcatttaaa tcaggtattt gctcatcttt tacatagttt
    14581 ctaacctgtc gagcggtaat acctaataac tcagctaatt ttgtttgcgt acaaccacgt
    14641 ttatccagcg caaattccag tctctcacga ttaaatgtct gcatgattta tcattcaaat
    14701 tatcctgctt tttgttttct ttcaaccaaa ggctcatatt cttcaacagg ttgcttacgt
    14761 tcaagctcat caaatttagt taaatcaaca tcagctaata taattcgctg cttgtaccca
    14821 gttatttgat gactaacaaa accactcggt aaagataatt caagttgcac tttattatac
    14881 ttccagtgaa acagcagaac ccaaaactgc acagtatcag gcaaatctag ttttgaatta
    14941 cgaatagcct cctcaaatcc cttacctttc cttgcggttg tcattggcat cccgtgatgc
    15001 ctaccaacat ctgaagtagc agtagccaca ataatacttt tagttcgaca tggcgagaga
    15061 catagaaatg caccacccga ccaaggctca agcgtccagc catctttact taaatatacc
    15121 cttagagcaa atgtaatttc tgcttgccga tacatcccca atgtatttct gtcagataat
    15181 gctgctttgt cctgaatatt attatgcgca gtaagtacaa tctctttaag catctcctga
    15241 gataagtact tgctgatttc acttaaagca atatcactat tttgttgctc gactatttct
    15301 cctacttcaa atgggaaagg ttctgataat gcaaattcca ccataaaaat ttcctaattt
    15361 tatacgtaat gtttacacaa tatatcagga aatatgaaaa cgtacaacta tatctataaa
    15421 gcaattaata agtagcctgc ccaaccgtgt ccttatcttt cggcacaccc gacctgcaaa
    15481 tcacgcaaaa cttggaatcc gtgtgtaggg tgtgtgcggt acatacgcac gcagtctttt
    15541 taaaccacag cccttcccaa ctaaaccaaa aggtcgtctg aaccctattt tcagacgacc
    15601 ttttgccact ttgtaaaaca aatcttccca ccatcctctc cccaaacatc gcccgaacca
    15661 gtaaacttct catatttcaa caactccttg gaagcaaacc atgtctggta tctacctacc
    15721 ccgcctattc ccgccccata tcgccgaacg cggcctgttg tattttcagc agggcaaggt
    15781 tctcgatgtc cgaaaaactt ccgccgggca ttatcgggcg gaggtgtgcg gttcggaaaa
    15841 ctattgggta tagttgaagc tggatagtga tttgtatatt aaagacgaag gctgcaattg
    15901 tccttatatc taagagtgca aacatacctt aaattactat attgcatagg caaaatacaa
    15961 gcctataacg aattggaaac aaaatgccgt ctgaaaacat cttcagacgg cattataaaa
    16021 tctgttcacc ttttcagatg agtaatgtac acccttatac aatttttgct actatgcccc
    16081 ataaatccac ggctaaagat atccttatta tgtcctatga tttatcgaaa cgacttgtaa
    16141 tcggcttagc atcaagtgcc ctattcgact tatccgaatc ggataatata tttagaatgg
    16201 aaggggcaga aacctatagg caatatcaga gagaaaaaca aaaccatccc ctaaaaaagg
    16261 cgttgtcttt ccatttatta aaaaacttct gtcaatcaat gaaataaacc caaacgaccc
    16321 aacgattggg tttattcttt tatccagaaa caatccagat acagattacg agtcataact
    16381 ataggcttaa tattacacga ttctcattcc atcaaggcgg aaaaccgcac aaatactgaa
    16441 acactatcga tcgatttgta aacaagccta cttaagtaac ttgcagtcct tatcatttcc
    16501 tttaaaataa tccagcccgt cactacacga actggcggac ttcttgcaaa taaaggttac
    16561 tagattttca ttcatcttaa taataaaagg atttttatct ttatctatgg ctaccgcctt
    16621 caacatgaat ttactgtcta aagccccgcg cgcgattcca ttcaaacgga tacaaaagcc
    16681 ttctgcctct ttaatcggca aacttggcca cttggtagat gtttgtttaa acctcccatt
    16741 ctgcagataa aacttttcca taaaatgtgc attttctaac aaggctgccc gcactgcatt
    16801 tatctttgct ttctcaacat aattgcgata gctcggataa acaattaaag caagtacaga
    16861 caatatcaag accactgata ttaattcaac cagcgtaaac ccccgattat cagtcattac
    16921 tttacttcca ataagaacag attattcaac atatttcttt gaacagactt actatcccat
    16981 tcaacagtat gcatatttcc cactctattt tttagcggcc ggtata
//
LOCUS       DAWGRZ010000025        16896 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000025 DAWGRZ010000000
VERSION     DAWGRZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16896
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.025"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(49..1218)
                     /locus_tag="WHT44_000575"
     CDS             complement(49..1218)
                     /locus_tag="WHT44_000575"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ2532796.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPSIAYEELKNAVIEHIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLIALV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1401..1511)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1564..2460
                     /locus_tag="WHT44_000576"
     CDS             1564..2460
                     /locus_tag="WHT44_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ2532797.1"
                     /translation="MFRLQFRLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGLNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2504..3568)
                     /gene="tsaD"
                     /locus_tag="WHT44_000577"
     CDS             complement(2504..3568)
                     /gene="tsaD"
                     /locus_tag="WHT44_000577"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ2532798.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLLYPGGAKLSELAESGRFEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVR"
     gene            complement(3685..4872)
                     /gene="ccsB"
                     /locus_tag="WHT44_000578"
     CDS             complement(3685..4872)
                     /gene="ccsB"
                     /locus_tag="WHT44_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ2532799.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGDIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4865..6817)
                     /locus_tag="WHT44_000579"
     CDS             complement(4865..6817)
                     /locus_tag="WHT44_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ2532800.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTVSREGGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKGAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7094..7717)
                     /locus_tag="WHT44_000580"
     CDS             complement(7094..7717)
                     /locus_tag="WHT44_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ2532801.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7923..8552
                     /locus_tag="WHT44_000581"
     CDS             7923..8552
                     /locus_tag="WHT44_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ2532802.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(8673..11069)
                     /locus_tag="WHT44_000582"
     CDS             complement(8673..11069)
                     /locus_tag="WHT44_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ2532803.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYMQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11221..12336
                     /locus_tag="WHT44_000583"
     CDS             11221..12336
                     /locus_tag="WHT44_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ2532804.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHFIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12339..12938
                     /locus_tag="WHT44_000584"
     CDS             12339..12938
                     /locus_tag="WHT44_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ2532805.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12939..13586
                     /locus_tag="WHT44_000585"
     CDS             12939..13586
                     /locus_tag="WHT44_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ2532806.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13604..14149
                     /locus_tag="WHT44_000586"
     CDS             13604..14149
                     /locus_tag="WHT44_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ2532807.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14168..16453
                     /locus_tag="WHT44_000587"
     CDS             14168..16453
                     /locus_tag="WHT44_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ2532808.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAAAPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
BASE COUNT         4562 a         4802 c         3857 g         3675 t
ORIGIN      
        1 aatgccgtct gaactgttca gacggcattt ttgattttca aaccggaatt acagccccgc
       61 tgccgccctc aatgcagcag ctttgtcggt gcgctcccaa gtgaactcag gttcttcgcg
      121 gccgaaatgt ccgtaagcgg cggatttact gtaaatcggg cgcaagagat cgagcatttg
      181 gacgatgcct ttggggcgca ggtcgaaatg ttcgcgaact aaggcaatca gtttttcttc
      241 gctgattttg ccggtgccga aagtatcgat ggaaatcgaa gtcggttcgg caacgccgat
      301 ggcgtaggaa acttggattt ggcattgggt tgccaaacct gcggcgacga tgttttttgc
      361 gacatagcgg caggcgtaag cggcggaacg gtccactttg gacgggtctt tgccggagaa
      421 tgcgccgccg ccgtgcggag ccgcgccgcc gtaggtatcg acgatgattt tacggccggt
      481 caaaccgcag tcgccttgcg ggccgccgat aacgaagcgg ccggtcgggt tgatcaggta
      541 tttggtttcg tcggtcagca gttcagacgg cagaaccggt ttgatgatgt gttcgattac
      601 ggcgtttttc agctcttcgt aagcgatgga cggatcgtgc tgggtagaca ggacgacggt
      661 gtcgatgcgt tttactttgc cggtttcgct gtcgtaaacc acggtcagtt gggctttggc
      721 atcaggacgc agccaaggca ggcggccgtc tttgcgcaat tcgctttgac gctgcatcag
      781 gcggtggctg taatagatgg caaacggcat cagggtaggg gtttcgtcac aggcatagcc
      841 gaacatcaaa ccttggtcgc ccgcgccctg gttcaagtcg atgccttcgc cttcgttcac
      901 gccttgggcg atgtcggggg attgctggtc gtagtacacg ccgactgcgc agccgttggc
      961 atcaaagccc agctcggagg agttgtagcc gatgcgtttg atggtttcgc gtgcgacttt
     1021 gatgtagtct acttgggcgg tggtggtaat ttcgcctgcc aatacgcaca agcctgtgtt
     1081 gaccaaggtt tctgcggcga cacgtgcttt tgggtcttgc gccaagatgg catccaaaat
     1141 cgcatcggat acttggtcgg caactttatc cggatggcct tcggataccg attcggaagt
     1201 aaacagatat tcgctcattg agattttcct tgaaaaacaa accatcttct tcagacggca
     1261 tgttgtatga acataatgtc gacagcggga aatatagcaa aatttcccta ttcataccat
     1321 tcagttgaga aatattccca tttgaatagc actttggaat ctctgcccgt acgtttctta
     1381 caggcaaaaa aattcccgca tcaagcgggt ttggattgct ctggtgagcc acatcggctt
     1441 ttcaaccgtc caccttactt ttccttttga aaagcaggtt ggcatggaat tcccaactct
     1501 taatgcagca cgcatcgtag cagaaaaggc atattgccgc aatacttccc tttttcagac
     1561 ggcatgtttc gtttacaatt caggctgttt ccccctttgc gaaccgccat gcacatcctg
     1621 ttgaccgccc tgctcaaatg cctctccctg ctgccgcttt cctgtctgca cacgctggga
     1681 aaccggctcg gacatctggc gttttacctt ttaaaggaag accgcgcgcg catcgtcgcc
     1741 aatatgcggc aggcgggttt gaaccccgac cccaaaacgg tcaaagccgt ttttgcggaa
     1801 acggcaaaag gcggtttgga acttgccccc gcgtttttca gaaaaccgga agacatagaa
     1861 acaatgttca aagcggtaca cggctgggaa catgtgcagc aggctttgga caaacacgaa
     1921 gggctgctat tcatcacgcc gcacatcggc agctacgatt tgggcggacg ctacatcagc
     1981 cagcagcttc cgttcccgct gaccgccatg tacaaaccgc cgaaaatcaa agcgatagac
     2041 aaaatcatgc aggcgggcag ggttcgcggc aaaggaaaaa ccgcgcctac cagcatacaa
     2101 ggggtcaaac aaatcatcaa agccctgcgt tcgggcgaag caaccatcgt cctgcccgac
     2161 cacgtcccct cccctcaaga aggcggggaa ggcgtatggg tggatttctt cggcaaacct
     2221 gcctatacca tgacgctggc ggcaaaattg gcacacgtca aaggcgtgaa aaccctgttt
     2281 ttctgctgcg aacgcctgcc tggcggacaa ggtttcgatt tgcacatccg ccccgtccaa
     2341 ggggaattga acggcgacaa agcccatgat gccgccgtgt tcaaccgcaa tgccgaatat
     2401 tggatacgcc gttttccgac gcagtatctg tttatgtaca accgctacaa aatgccgtaa
     2461 cgaaaataaa aatgccgtct gaacaatttc agacggcatt ttgtcatctg acgatttccg
     2521 acagcggcca gcgcggacgg acattgaacg caccgacctc cctgcccttg cccaggtgca
     2581 tcgcaccggc aaaggcaatc atggcaccgt tgtccgtgca gtatgccgtc ggcgggaaaa
     2641 acacgctgac tttttcggac ggatgtttcg gcttgccttt gggggtcggg atttgcaccg
     2701 tcatgttgcc gaaagtttca cggagcttgc ggtttgcacc gaccccgccg gcgaccacta
     2761 cggttctgaa ccctgtctgc aacagggctt ttttcacttt cgccgccaac acatcgacta
     2821 ccgcatcttg aaacgcacgg cagatgtcgt tgcgtgtctg ctcaggaatg tcatccgccc
     2881 cgttttccgc gcgcactttc tcgacggcgg tcaatacggc ggttttcaaa cctgaaaaac
     2941 tcatctgcaa atcgtcggaa tgaatcatcg ggcgcggaaa aacaaacgct tcgaacctgc
     3001 ccgattccgc aagttccgac agtttcgcac cgcccggata cagcaagccc agcagtttcg
     3061 ccgttttgtc gaatgcctcg cccgccgcat catcgacgct ctcgcccaaa agcgcgtagt
     3121 cgcctatgcc cctgaccgcc ataatctgcg tatgcccgcc cgaaaccaac agcgcgacaa
     3181 aaggaaagtc gggtttttcc tccgccaaca gcggcgacag cagatgtcct tccaaatgat
     3241 ggacgggaat aacaggcttg tccaacgcta aagccagcgc gttggcgtag ctcgaacccg
     3301 ccagcagcgc gccgcccaaa ccgggcccct gcgtaaaggc aaccgcgtca atgtcgccat
     3361 acgatgcgcc tgcctgcgcc agacagcctt ccgtcaacgg aacaaggcgg cggatatggt
     3421 cgcggcttgc caattccggc acaaccccgc cgtattcggc gtgcattgcc atttgagtgt
     3481 gcaggcagtg cgcccgcaat ccacgttccg tatcgtaaag cgcaacacct gtttcgtcgc
     3541 aagaagactc gattcctaat accaacatgg tctgatgccg ttaaaaactg aaaaacgtat
     3601 tttagcggat ttcggcacga ctgccgtatc ccaaaaacgg aacatgccgt ctgaagaccg
     3661 ttcagacggc atcgtcgcac cgtatcaaag cgttccgtaa gaatgcagcc cgctcaaaaa
     3721 catattcacg ccgataaagg caaatgcggt tacgaacaaa ccgataatcg cccaccacgc
     3781 cagcactttg ccgcgccaac cggcaaccag ccgcaagtgc agccaaacgg cgtaattgag
     3841 ccagacgatg aacgcccacg tctctttcgg atcccaactc caatagcgtc cccaagcatc
     3901 tgccgcccac agcgcaccca aaatggtggc aatggtaaag aacagaaagc cgacggcaat
     3961 cgccttatac atcacctcgt cgatcaatgc cgacggcggc agccacagtt ttccgccttt
     4021 tccttccgca cgcagggaaa ccagttcggc aataccgagc atcgcggaaa tgcaaaacgc
     4081 gccgtaaccg ataaagtttg ccggaacgtg gattttcatc caccaggact ggagcgcggg
     4141 aatcagcggc tggatggtat gcgcctcgcg ggacacgctg taccacaaga caaatccaac
     4201 cacgaccgcc ataaagccga acacgaagcc gcccaatttc tgtatggcga acttaccttc
     4261 ataataaaga tacatcagcg cggtaatcac caaaaacagg atgaacactt catacaggtt
     4321 ggaaaccgga atatgccccg catcgggacg gagcagatag ctttcgtgcc aacgtaccag
     4381 cagaccggta aagcctgcta cggcagacac ccatgcaaac acggttccca tacccaacag
     4441 cgtgttggtc ggcacatttt ttacgcttgc caaaaccgcg cccgaaatat aggcgaacag
     4501 ggcgaaaaag acaaaggcgc actgccacat gatcgccgac tggctgctga ggaaataccg
     4561 caacaggaaa atctctgccg atttgatgtc gcctccgtac aaaccgacgg cggcataggc
     4621 aagcaatacg cttaaaggaa caaaccagcg catcggtttg aaaaaccaac ccaaaaacac
     4681 ggcaataccg gcacttgccc acaacatgac cgtttcgtaa atgtccatat gcataccgga
     4741 acgggtctgt acgaaaaccg tagccgcaaa aaccagcacg gcaaataccc aatcccaaag
     4801 attcagattg ctgatcaaag acttctgaat cagcagctcg tgttccggaa gggttttata
     4861 gtgttcagtc atgattcaag tccttgccga gccgttgcag actctcgacg tgttttggaa
     4921 attccttctg caaatcccgt tcgctgcggg ccgaagacat ggcaaaacgg attttgccgt
     4981 ctgaaaacaa tacccacgcc cgtttttcgc gcacataaaa catcaatacc gtacccaata
     5041 ccaacagcac cgagccgaga tagaccaaaa gcgcacccgg ggaacgggtc atctgcaaac
     5101 ccgacgaacg cacctcggaa aacccatcaa gttgcagcag cataggcgcg ggatattcgg
     5161 tcaaacccgt gtacgcatcc atactgtgca gcaggaaacg attccgcgct tcatcctgct
     5221 gccattcggg caagccgtac cggcgtatgg tttcatccaa agcagcgttc atcacgccgt
     5281 aaagcatttc gtagaaatag ccctgcatct tatcctgctg ctctttcggg atattggacg
     5341 taataaattc gtccaatccc aaatagcctt tttgtgcaaa gatgttcagc gtgttttccg
     5401 cagccagcat gaattgttcg cggatttcgg caggtgcgcc tttggttgcg tcggcaacca
     5461 gacgtttgcg cccttcccca tctttcaaaa actcacgcaa tgccataaag gtgtccgctt
     5521 tcaactgctt gtccaagggg atacgcagcc agcggtattg ctgctgcaag ccgctgcgcg
     5581 tgccggtaat ccaaaaataa tcctgttcct gcaaaaccgg cagcatatag tttttatatt
     5641 cgaccgcctg ccctgccgca tcacggatac ggtaaacaat ggaagggccg atattggtgt
     5701 attttttacc ttcctgagta acggcgcgga catcgttcag cgtggatttc aggctttttt
     5761 cccgttccgc gccctcgctc atgtcctcca cattcataga agtgaactga tcgaactcaa
     5821 gacgatattt gtgtttgcca atttccaacg gaaactggtg tatggatgtt gccttcaaca
     5881 cgacaggctc gcgcgaagca tcacccaaat tccacgcctt gaatgtcaaa tccgaaccgc
     5941 cgtcggcaaa actcgcctga taaatcgtga tgccgtgcaa ggtcaaagga tggttcacgc
     6001 ggatggtgcg ctcgagtttc tcaccggttg ccttgtccgt cacttcaata tcgctggcga
     6061 aatcacgcgg catacccgta ttgtaaaaat cgatatggaa ttttttcagt ttgacttcaa
     6121 aaggcaagtc ctgaaccaat atcccgttgt cggcattcag gaaaaccaca tccgcactct
     6181 gcccctcgga aatattgacg ttgcccctaa atgagagatt ggacgcaccc aaaatacttt
     6241 cgggcttgaa atccttggca taaaccgcct gattgtccgg aacaatccga ccggtcagca
     6301 tacccagttt caacagcagg ttactgtcta tcaacccgcc caggcaaatg acaatcaaag
     6361 caacatgggc aaagatatag ccccatttgt tcatcgtgcc ttttttggcg gcaatcagaa
     6421 ccgacccgcc ctcacggctg acggtttttc cctgaaaacc ctgcacctcc agataacgtt
     6481 tggcaacctc gggcgcaatt tttacatcca acagcgaaga atggcgcatc gccgccagag
     6541 atttttcttt aaccttttcc cgaaaagact tcatttcgcg ccagaacggc ggcacattgc
     6601 gaatcaggca caaactggta gaaaccacca aaaacatcat gataacgaca aaccatgccg
     6661 aagcatagac gtcatacagt cccagaaaac caaaaatctg cgcccaaaaa ggtccgaatt
     6721 tgaccaaata atccgtctgc ggctggtttt gctgcaacac cgtaccgata accgatgcaa
     6781 tacccagcag actgagcaaa gcgactgcaa agcgcatgga gctgaaaaaa gcgaaccacg
     6841 gacgggaaag aagtggggga gatctacggg atttactcat tgtgtgttta ttccgccatc
     6901 aggaatatgg gaaagcagaa ttgggcaaac agaaaacaac gtcccgattc tactgtcttg
     6961 atgcttttta tttcaagaca atgaagacag cctgcatcga ttccaacggt tgcgattgaa
     7021 aaaacttatc gcagaattgc ctgaagccgt ctgaaaactt ttcagacggc ctctaaaaca
     7081 gactattgcg gaattaacgc aaaccttgga taaagttggc gaccgctttc aaatcttctt
     7141 cagacatacg gtttgcaata tcttccatga tggtattttt acgctgaccg gacttgtagg
     7201 cattcatctg ttcaacaata tatgcctgat gctgaccgcc caaacgcgga taagcctgaa
     7261 tttcgcttcc gcctcccggc atacccgcac cgctcggacc gtggcaggac atacacgccg
     7321 gcactttttt atcgctcaaa ccgccgcgat agattttcgc acccaattcg ggattttcct
     7381 taggattggc ttcaccggat ttgggctgct gtttggcata gaatgcggat acgttcaaaa
     7441 tatcctgatc gctcaaattc attaccaccg gtttcatcac agctgccgaa ccgtgggtac
     7501 gtttgccgtc gcggataccg atagtttgat gatagatgta agcagtatgc tgtgccgcca
     7561 aacgtggata catcgcaatg ccgctattac cgtctgctgc atggcaagcc gcacaaaccg
     7621 ttgcggcaac ctgtttgcct ttttccacgt ctgctttggg agatgcggaa accgcaccgg
     7681 cagccaaaac aaaggccaat aaagtcaatc gtttcatgga gtgctcctga ttacagcatt
     7741 ggataacgca acaatgctct ttttatattc aaatacggga tttttgaccc gattaaaacc
     7801 gatgattctg taaacgtgtt attctatact aaatttacat taaattacca ctgtgtttca
     7861 cataaaacca accgcatatt tttgctgtcg gacaaacggc ggcggaaaac aaggatatgc
     7921 ccatgaacct ttttcaaaac gccaaattct tcacgacggt aaaccatctc aaagacctgc
     7981 ccgacacccc tctcgaaatt gcctttgtcg gcaggagcaa tgccggaaaa tccagtgcca
     8041 tcaataccct gaccaaccat gtccgtcttg cctacgtttc aaaaacaccc ggacggacgc
     8101 agcatatcaa cttcttcgag ctgcagaacg gcaattttat ggtcgatttg cccggctacg
     8161 gttatgccca agtccctgaa gcagtacgcg cacattgggt caatctgctc ggcgactatc
     8221 tgcaacagcg caaacagctt atcgggctgg ttttgattat ggatgcccgc catcctttaa
     8281 aagaactcga catccgtatg ctggattttt tccacacgac cggcagaccg gttcacatcc
     8341 tgctgtcaaa agccgacaaa ttatccaaaa acgaacagat aaaaaccctg tcccaagtca
     8401 aaaaactgct caaaccttat tccgacaggc aaaacatcag cgtacagctg ttttccagcc
     8461 tgaaaaaaca aggtattgac gaggccaacc gaactgtcgg aagctggttt gacgcagcag
     8521 atgccgccgc ttcctctcca gaggaaaact gaccccaatt atacggaaac cgtatttccc
     8581 ccacttgacc gaccgcaaac atttaaaaaa ttgccactgc caaatctaaa atgccgtctg
     8641 aaagactttt cagacggcat tttgcggagt ctttaaaaca gagaatccaa ctgctgctgt
     8701 ttggaaccag tattactcgg aagcaccgga gtttcctgca tatcttggcg gacttcgtca
     8761 tccgccgcct gccgtccgcc ttctgccgcg cccccgtcat cttcttttgc ccgtcgggaa
     8821 ggttgcggcg caataccgct gttgtccagc gtcaagcccg gatcggttac catacgttcc
     8881 ttcatatagt attcgccatt gctgctgacc acaccttcag gcattttcat ccccttgccc
     8941 tgctttcctt tcaacgcaaa acgcatatag tccacccaaa ccggcaccgc aatcgtaccg
     9001 ccgtagccga cacgccccat actcttaggt ttgtcgaagc cgatatatac ggcagtaacc
     9061 acatcagggt taaaaccgac aaaccacgca tccttattgt cattggtcgt acccgtttta
     9121 ccggcaatat ccgttcttcc caacgcagct gccccccttg ccgtaccgac acggaccaca
     9181 tcctgcataa tcttatacat aatataggca ttgcgcggat cgattgcctg aggcgcattt
     9241 tgcccagcca ccaaaggttg catttgggcg cgcaacctgc cgtctctgtc ataaatctta
     9301 tcgattacgt gcgaagaaac cctatatccg ccgttcgcaa atacgctata tgcctccgcc
     9361 actttcaacg gcgttgtctc gcccgtacct aaagccatag acaggcttgc cggcagctcg
     9421 gacgacctga agccgaaacg ccggatatac tgttgcgcgt aaccgacacc gatagacatc
     9481 aaaatacgga tggaaaccat attcttggaa gccgtcagag cctgtctcaa agtaatgtag
     9541 ccggaatatc tgccgtctga atttttaggt gtccaaaccg aaccgttcgg ccctttcccc
     9601 ggcagggaaa tcggcgcatc gttaaccact gtggacgcgg tcatcccctt agataatgcc
     9661 gccgaataga caaacggctt aaaggtcgaa cccggctgcc gcattgcctg aacggcacga
     9721 ttgaatgttt tgctgtgaaa atcataaccg ccgaccagcg cgcgcacagc tccggttttt
     9781 gcatccagcg aaaccaaagc cccctgcagc aacggctctt gaaccaccgc ccaacgcccg
     9841 ccgttgtttt tgacacggat gaccgcgccc ctgcggatac ggtcctcccc cattttttca
     9901 ttattgaccg cgcgggccgc aaagcccaag gcgcgcctat caagcgtaac ccgcctgccg
     9961 ccgggcagct gtatgacgac atttttcttt ttagtcacat ccaacacaac ggcgggaacc
    10021 attttatcga cggtatagag tcccgacaga tactggctga cagtctcctc gacatcttca
    10081 ctcttactca aatcgatata gttttccgca ccgcggtagc tgctgccgcg atcgaaattc
    10141 cgtagagcct tgcgcaatgc ctcggttgcc accttctgat gatcggcgcg gaccgtggta
    10201 taaaccttaa aaccctgcat ataggcatct tcaccgtatt tctcatacag ttcctgacgc
    10261 accatttccg ccacatataa agcactctga tcgattttcc gaacaaaccg ctcgtaatgc
    10321 agttcctcat tcaacgcctg atcgcgctgt tgcacggtaa tcatcttctc ctcgagcata
    10381 ttgttcaaaa tatacttctg gcgcaacttg gcacgttctg gattaacaat cggattatag
    10441 gcagacggag ccttgggcag tcccgcaagc atggcggctt ccgccaaagt caaatctcgg
    10501 acattcttat tgaaatagat ttgcgcggca gatgcaaaac cataggcgcg ctgaccgagg
    10561 taaatctgat tgaaatacaa ctcgaggatt ttgtctttgc ttaaagactg ctcgatttta
    10621 taggcaagca acacctcatt gaatttgcgt gtgaacgttt tttcactgct caaataaaaa
    10681 tttttcgcca cctgctgcgt aatcgtactc gcacccgact gcacgctgcc ggacacgaca
    10741 ttgccgacgg cagcgcgggc aacaccccaa acatccaccc cccaatgccg gtaaaagcgt
    10801 ttgtcctcgg cggcgataac cgcattccgc aacacctctg ggaaatcgcc gatttttgta
    10861 aattcgcgcc gctgctcccc atacataccg atgacttccc catccgccga ataaatagtc
    10921 aacggcattt taggctggta atgctgcaaa gaatccaaag acggcagttt cggatacgtt
    10981 accaaaatag caatggcaac caaacccact ccaaatacac aaaacccaaa aaccaaacca
    11041 aaacaagtcg ttaaaatctt tttaatcata gctgaataat aatttaccat tattggtatt
    11101 aaataaagta aaatagcaac cgatttctac aaagcacggt ttcaatgtgc aaagaacaag
    11161 gaatccatta cggataccga aacggttact cactgtacaa ataaagcagg aactttcatc
    11221 atgcgcttgt ttaaaagctt gaaaaaccct aaaaaaacag atgccaagct ccctaaaaaa
    11281 tcttcgggac tcaataaccg cgcggcaatc ggcatcgata tcgaccagca tttcatcaaa
    11341 atggtccaat tgtcaggacg tagtttaaac caaattcaat tggaaaaata cgtcattgcc
    11401 aaattaccaa agaatatcat tcaaggcaat aaagtccaaa attacgatca acttgttaca
    11461 tatttgcaac aagcctatgc caaactgggt acttcgtgca aaaacatcat cgcgtccgtc
    11521 ccgcaaaatc tggcaaccat cgaacaattg acctacacag acaaagatgc agaattagac
    11581 ctgcaggggt tcgtggagtc ctccatctcc gaagtcagct cgatatcgct cgaagaagcc
    11641 aattacgact atcaggtctt gtcccaatcg gccgccggcg aagctgtgtt ggccgtcgca
    11701 tcgagaaagg atgaaatcga acccctgatt gacgcattca acgccgccgg tatgaaatta
    11761 tccgcgcttg atgtggacat tttcggacaa tacaacgcct acgcgctatg gataaaccat
    11821 ttcgcccccg agcttgcagc cgaaaaagtc gccattttcg gcgtatatgc cgcacagacc
    11881 tacgccttgg tcatccaaga cggaaaaatc ctatacaaac aggaaacctc cgtcagcgaa
    11941 gaacagctca accaactcat ccagcgcacc tatcaggtaa cagaagaaaa agcggaagaa
    12001 atcatcaact ccccgcaaaa accttccgat taccaagaaa gcgtggcaaa ctatttcaac
    12061 cagcagatta cccaagaaat acaaagggtc ttgcagtttt attacaccac gcagaccgca
    12121 gacgatatga ccgacatcaa gcatatcctg ctgaccgggg aagcggcgcg ccaggaaggc
    12181 atcgcccaaa ccgtcgcctc acaaaccaat gcagatgtac aatgcgtcca tcccgcgcgt
    12241 tattttgcgg acaacctcaa aacagacaaa caacaattcg aacttgatgc gccgacactg
    12301 accagggcgt tcggtttggc ggtacgggga ttataattat gaacaattta atcaaaatca
    12361 acctcctccc ctacagggaa gagatgaaca agcgcaaaca gcagcagttt aaaacgctga
    12421 tgtacggtgc cgtgctgacg ggcgttgccg ccgttgcggc aacctacctg tttatcgaca
    12481 atatgatcaa taaccagtcg gaaagaaaca cgctgctgga aacctccatc gcacacttgg
    12541 ataccgagct gtcggaaata caaaagctca aacaggaaaa agatgccttc ctgattaaga
    12601 aaaacaaaat cgaggagctc cagctcaaac gcctccaagc cgcaaaaatc ctcgacagcc
    12661 tgaatgaggc cgtccccgga agcacctacc tgacctcgct ggatgccgtt accgccgact
    12721 cttatcggct cagcggcagg acatccagcg acaaccgcgt tgccgccatg atgagggcga
    12781 tgcccaatac cggcatattc aagcaacccg aattgttaag catcaagaaa aacaattcgc
    12841 atcaagaatt tacccttcag gcaacattac aacccatcgt aaaggcggcc gaatccaaag
    12901 agaatccggc ttcgggaaac gcacaggagg caaactgaat ggcttctaaa tcatctaaaa
    12961 ccaacttgga tctcaacaac cttcacctgc tcaaccttcc tgccaggctt tttatcgccc
    13021 tgctggccgt tgccgccgtg ctggggctcg gttatgccgg attgttcaaa agccagatgg
    13081 aatcccttga ggaatacgaa gcaaaagaaa ccgaactgaa aaacacctac aaacagaaaa
    13141 gtatcgacgc ggccagcctg aacaacctga gggacgaact tgcctcaatc cgctctgcct
    13201 tcgatatcat gttgaaacag ctgccgacag atgcagaaat tcccaacctg gttcaagagc
    13261 ttcatcaggc aggttcgagc aacggcctgc gcttggacag cgttatgccc caacctcccg
    13321 tagatgacgg ccccatcaaa agattaccct attccatttc cattaccgga aattacgaac
    13381 agatcagcca atttacccgc gatgtcggca gcctctcccg aatcattacc cttgagtcgc
    13441 tgaaaatcgc ccaatctccg gaaaacggcg gcaatcctga cggcaagagc agcatcctga
    13501 acctcagtgc cattgccacc acctaccaag caaaatccgt agaagagctt gccgcagaag
    13561 cggcacaaaa tgccgagcaa aaataactta cgttagggaa accatgaaac actatgcctt
    13621 actcatcagc tttctggctc tctccgcgtg ttcccaaggt tctgaggacc taaacgaatg
    13681 gatggcacaa acgcgacgcg aagccaaagc agaaatcata cctttccaag cacctaccct
    13741 gccggttgcg ccggtataca gcccgccaca gcttacaggg ccgaacgcat tcgacttccg
    13801 ccgcatggaa accgacaaaa aaggggaaaa tgcccccgac accaagcgta ttaaagaaac
    13861 gctggaaaaa ttcagtttgg aaaatatgcg ttatgtcggc attttgaagt ctggacagaa
    13921 agtctccggc ttcatcgagg ctgaaggtta tgtctacact gtcggtgtcg gcaactattt
    13981 gggacaaaac tacggtagaa tcgaaagcat taccgacgac agcatcgtcc tgaacgagct
    14041 gatagaagac agcacgggca actgggtttc ccgtaaagca gaactgctgt tgaattcttc
    14101 cgacaaaaac accgaacaag cggcagcacc tgccgcagaa caaaattaag aagaggatta
    14161 ctccattatg aataccaaac tgacaaaaat catttccggt ctctttgtcg caaccgccgc
    14221 ctttcagaca gcatcggcag gaaacattac agacatcaaa gtttcctccc tgcccaacaa
    14281 acagaaaatc gtcaaagtca gctttgacaa agagattgtc aacccgaccg gcttcgtaac
    14341 ctcctcaccg gcccgcatcg ccttggactt tgaacaaacc ggcatttcca tggatcaaca
    14401 ggtactcgaa tatgccgatc ccctgttgag caaaatcagt gccgcacaaa acagcagccg
    14461 tgcgcgtctg gttctgaatc tgaacaaacc gggccaatac aataccgaag tacgcgggaa
    14521 caaagtttgg atattcatta acgaatcgga cgataccgtg tccgcccccg cacgccccgc
    14581 cgtaaaagcc gcgcctgccg caccggcaaa acaacaggct gccgcaccgt ctaccaagtc
    14641 cgcagtatcc gtatccaaac cctttacccc ggcaaaacaa caggctgccg caccgtttac
    14701 cgagtccgta gtatccgtat ccgcaccgtt cagcccggca aaacaacagg cggcggcatc
    14761 agcaaaacaa cagacggcag caccagcaaa acaacaggcg gcagcaccag caaaacaaac
    14821 caatatcgat ttccgcaaag acggcaaaaa tgccggcatt atcgaattgg ctgcattggg
    14881 ctttgccggg cagcccgaca tcagccaaca gcacgaccac atcatcgtta cgctgaaaaa
    14941 ccataccctg ccgaccacgc tccaacgcag tttggatgtg gcagacttta aaacaccggt
    15001 tcaaaaggtt acgctgaaac gcctcaataa cgacacccag ctgattatca caacagccgg
    15061 caactgggaa ctcgtcaaca aatccgccgc gcccggatac tttaccttcc aagtcctgcc
    15121 gaaaaaacaa aacctcgagt caggcggcgt gaacaatgcg cccaaaacct tcacaggccg
    15181 gaaaatctcc cttgacttcc aagatgtcga aatccgcacc atcctgcaga ttttggcaaa
    15241 agaatccggg atgaacattg ttgccagcga ctccgtcaac ggcaaaatga ccctctccct
    15301 caaagacgta ccttgggatc aggctttgga tttggttatg caggcacgca acctcgatat
    15361 gcgccaacaa gggaacatcg tcaacatcgc gccccgcgac gagctgcttg ccaaagacaa
    15421 agccttctta caggcggaaa aagacattgc cgatctgggc gcgctgtatt cacaaaactt
    15481 ccaattgaaa tacaaaaatg tggaagaatt ccgcagcatc ctgcgtttgg acaatgccga
    15541 cacaaccgga aaccgcaata cgcttgtcag cggcaggggc agcgtgctga tcgatcccgc
    15601 caccaatacc ctgattgtta ccgatacccg cagcgtcatc gaaaaattcc gcaaactgat
    15661 tgacgaattg gacgtacccg cgcaacaagt gatgattgag gcgcgtatcg tcgaagcggc
    15721 agacggcttc tcgcgcgatt tgggcgttaa attcggcgcg acaggcaaga aaaagctgaa
    15781 aaatgataca agcgcattcg gctggggggt aaactccggc ttcggcggcg acgataaatg
    15841 gggggccgaa accaaaatca acctgccgat taccgctgcc gcaaacagca tttcgctggt
    15901 gcgcgcgatt tcctccggtg ccttgaattt ggaattgtcc gcatccgaat cgctttcaaa
    15961 aaccaaaacg cttgccaatc cgcgcgtgct gacccaaaac cgcaaagagg ccaaaatcga
    16021 atccggttac gaaattcctt tcaccgtaac ctcaatcgcg aacggcggca gcagcacgaa
    16081 cacggaactc aaaaaagccg tcttggggct gaccgttacg ccgaacatca cgcccgacgg
    16141 ccaaatcatt atgaccgtca aaatcaacaa ggactcgcct gcgcaatgtg cctccggtaa
    16201 tcagacgatc ctgtgtattt cgaccaaaaa cctgaatacg caggctatgg ttgaaaacgg
    16261 cggcacattg attgtcggcg gtatttatga agaagacaac ggcaatacgc tgaccaaagt
    16321 ccccctgttg ggcgacatcc ccgttatcgg caacctcttt aaaacacgcg ggaaaaaaac
    16381 cgaccgccgc gaactgctga ttttcattac cccgaggatt atgggtacgg ccggcaacag
    16441 cctgcgctat tgatgcgtca aaataagggc atatgtttta cggcatatgc cctttcttta
    16501 tgctttttgc cgcgaccgaa atgccgtcat tcccgcgcag gcgggaatcc agtccgttca
    16561 gtttcggtca gtttcggtca tttccgataa attcctgttg cttttcattt ctggattccc
    16621 acttttgtgg gaatgacggc ggaaggggta aatcctcaca acccaaagcc tcgtcatttc
    16681 cacaaaaaac agcaacccga aacagcaact taaaaccccg tcattcccgc gcaggcggga
    16741 atctaggtct gtcggttcag gaacttatcg gataaaacgg tttctccaac cctgcgttct
    16801 agattcccac tttcgtggga atgacgggat atgggtttcc gtgcggacgt gttcggattt
    16861 ccgcctgcgc gggaatgacg gcgacagatg cccaac
//
LOCUS       DAWGRZ010000026        16814 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000026 DAWGRZ010000000
VERSION     DAWGRZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16814
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.026"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            336..980
                     /gene="rplC"
                     /locus_tag="WHT44_000588"
     CDS             336..980
                     /gene="rplC"
                     /locus_tag="WHT44_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ2532809.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            980..1600
                     /gene="rplD"
                     /locus_tag="WHT44_000589"
     CDS             980..1600
                     /gene="rplD"
                     /locus_tag="WHT44_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ2532810.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            1597..1911
                     /gene="rplW"
                     /locus_tag="WHT44_000590"
     CDS             1597..1911
                     /gene="rplW"
                     /locus_tag="WHT44_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ2532811.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVADVTTVTIKGKVKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            1917..2750
                     /gene="rplB"
                     /locus_tag="WHT44_000591"
     CDS             1917..2750
                     /gene="rplB"
                     /locus_tag="WHT44_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ2532812.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLYKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVDCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            2757..3035
                     /gene="rpsS"
                     /locus_tag="WHT44_000592"
     CDS             2757..3035
                     /gene="rpsS"
                     /locus_tag="WHT44_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ2532813.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3044..3373
                     /gene="rplV"
                     /locus_tag="WHT44_000593"
     CDS             3044..3373
                     /gene="rplV"
                     /locus_tag="WHT44_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ2532814.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            3383..4075
                     /gene="rpsC"
                     /locus_tag="WHT44_000594"
     CDS             3383..4075
                     /gene="rpsC"
                     /locus_tag="WHT44_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ2532815.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4059..4475
                     /gene="rplP"
                     /locus_tag="WHT44_000595"
     CDS             4059..4475
                     /gene="rplP"
                     /locus_tag="WHT44_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ2532816.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            4475..4666
                     /gene="rpmC"
                     /locus_tag="WHT44_000596"
     CDS             4475..4666
                     /gene="rpmC"
                     /locus_tag="WHT44_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ2532817.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            4666..4929
                     /gene="rpsQ"
                     /locus_tag="WHT44_000597"
     CDS             4666..4929
                     /gene="rpsQ"
                     /locus_tag="WHT44_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ2532818.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5151..5519
                     /gene="rplN"
                     /locus_tag="WHT44_000598"
     CDS             5151..5519
                     /gene="rplN"
                     /locus_tag="WHT44_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ2532819.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            5531..5854
                     /gene="rplX"
                     /locus_tag="WHT44_000599"
     CDS             5531..5854
                     /gene="rplX"
                     /locus_tag="WHT44_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ2532820.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            5864..6403
                     /gene="rplE"
                     /locus_tag="WHT44_000600"
     CDS             5864..6403
                     /gene="rplE"
                     /locus_tag="WHT44_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ2532821.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            6406..6711
                     /gene="rpsN"
                     /locus_tag="WHT44_000601"
     CDS             6406..6711
                     /gene="rpsN"
                     /locus_tag="WHT44_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ2532822.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            6727..7119
                     /gene="rpsH"
                     /locus_tag="WHT44_000602"
     CDS             6727..7119
                     /gene="rpsH"
                     /locus_tag="WHT44_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ2532823.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7133..7666
                     /gene="rplF"
                     /locus_tag="WHT44_000603"
     CDS             7133..7666
                     /gene="rplF"
                     /locus_tag="WHT44_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ2532824.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGAEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            7680..8033
                     /gene="rplR"
                     /locus_tag="WHT44_000604"
     CDS             7680..8033
                     /gene="rplR"
                     /locus_tag="WHT44_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ2532825.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8052..8570
                     /gene="rpsE"
                     /locus_tag="WHT44_000605"
     CDS             8052..8570
                     /gene="rpsE"
                     /locus_tag="WHT44_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ2532826.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            8563..8748
                     /gene="rpmD"
                     /locus_tag="WHT44_000606"
     CDS             8563..8748
                     /gene="rpmD"
                     /locus_tag="WHT44_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ2532827.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLCRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            8750..9184
                     /gene="rplO"
                     /locus_tag="WHT44_000607"
     CDS             8750..9184
                     /gene="rplO"
                     /locus_tag="WHT44_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ2532828.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            9196..10506
                     /gene="secY"
                     /locus_tag="WHT44_000608"
     CDS             9196..10506
                     /gene="secY"
                     /locus_tag="WHT44_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ2532829.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            10511..10729
                     /gene="infA"
                     /locus_tag="WHT44_000609"
     CDS             10511..10729
                     /gene="infA"
                     /locus_tag="WHT44_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ2532830.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            10750..10863
                     /gene="rpmJ"
                     /locus_tag="WHT44_000610"
     CDS             10750..10863
                     /gene="rpmJ"
                     /locus_tag="WHT44_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ2532831.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            10929..11291
                     /gene="rpsM"
                     /locus_tag="WHT44_000611"
     CDS             10929..11291
                     /gene="rpsM"
                     /locus_tag="WHT44_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ2532832.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            11311..11706
                     /gene="rpsK"
                     /locus_tag="WHT44_000612"
     CDS             11311..11706
                     /gene="rpsK"
                     /locus_tag="WHT44_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ2532833.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            11726..12346
                     /gene="rpsD"
                     /locus_tag="WHT44_000613"
     CDS             11726..12346
                     /gene="rpsD"
                     /locus_tag="WHT44_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ2532834.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            12372..13358
                     /gene="rpoA"
                     /locus_tag="WHT44_000614"
     CDS             12372..13358
                     /gene="rpoA"
                     /locus_tag="WHT44_000614"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ2532835.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            13382..13750
                     /gene="rplQ"
                     /locus_tag="WHT44_000615"
     CDS             13382..13750
                     /gene="rplQ"
                     /locus_tag="WHT44_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ2532836.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHEAIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            13897..14610
                     /gene="minC"
                     /locus_tag="WHT44_000616"
     CDS             13897..14610
                     /gene="minC"
                     /locus_tag="WHT44_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ2532837.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQKTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            14638..15453
                     /gene="minD"
                     /locus_tag="WHT44_000617"
     CDS             14638..15453
                     /gene="minD"
                     /locus_tag="WHT44_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ2532838.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            15457..15720
                     /gene="minE"
                     /locus_tag="WHT44_000618"
     CDS             15457..15720
                     /gene="minE"
                     /locus_tag="WHT44_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ2532839.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            15724..16644
                     /gene="oxyR"
                     /locus_tag="WHT44_000619"
     CDS             15724..16644
                     /gene="oxyR"
                     /locus_tag="WHT44_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ2532840.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFICD"
     gene            complement(16641..>16814)
                     /locus_tag="WHT44_000620"
     CDS             complement(16641..>16814)
                     /locus_tag="WHT44_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532841.1"
                     /translation="WHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNILAYRVV
                     GSGKWRFENNACA"
BASE COUNT         4613 a         3148 c         4175 g         4878 t
ORIGIN      
        1 cacagcctca tcacccgcat cgatttcaac ctgttttgcc gctttgacga actgagcatc
       61 cccgattaca gtcatcaacc atattccggt ttgtcggaga aagatgcata cgctgtgatg
      121 accggatacc gacccgttaa aagagtccga ccctatgccg tctgaaaatt caaaacgctt
      181 cagacggcat attgaagata tttctgatat ttctgttgat atttctttga cttgtcagat
      241 ataatgccga gcttggtaca tttgtgccaa gtttaacttt gtctgaaaga caggccaatc
      301 gtagcctgtc cctttacttt aaaaggaaaa taatcatgac tttaggtctg gttggacgca
      361 aagttggtat gacccgcgtg ttcgacgaac agggtgtttc tgttccggta accgttttgg
      421 atatgtctgc caaccgcgtt acacaagtaa aatccaaaga tactgacggc tatactgccg
      481 ttcaagttac ctttggtcag aaaaaagcca atcgtgtcaa caaagccgaa gccgggcact
      541 ttgcaaaagc aggtgttgaa gccggtcgcg gtttgattga gtttgctttg actgaagaaa
      601 aactggctga attgaaagct ggtgacgaaa tcaccgtttc tatgtttgaa gtcggtcaac
      661 tggtcgatgt aaccggtacc tctaaaggta aaggtttctc cggcacgatt aaacgtcata
      721 acttcggtgc ccaacgtact tcccacggta actcccgttc tcaccgtgtt ccaggctcta
      781 tcggtatggc gcaagacccg ggtcgcgtgt tccccggtaa acgcatggcc ggccaatacg
      841 gcaacaccaa agcaactgtt caaaaattgg aagttgtccg tgttgacgca gaacgccaac
      901 tgctgttggt taagggtgct gttccgggtg cggtcaacag cgatgttgta gttcgtccca
      961 gcgtgaaagt aggtgcgtaa tggaattgaa agtaattgac gctaaaggac aagtttcagg
     1021 cagtctgtct gtttctgatg ctttgttcgc ccgcgaatac aatgaagcgt tggttcatca
     1081 gctggtaaat gcctacttgg caaacgcccg ctccggtaac cgcgctcaaa aaacccgtgc
     1141 cgaagtaaaa cactcaacca aaaaaccatg gcgtcaaaaa ggtaccggcc gtgcccgttc
     1201 cggtatgact tcttctccgc tgtggcgtaa aggtggtcgc gcgttcccga acaaacccga
     1261 cgaaaacttc actcaaaaag taaaccgcaa aatgtaccgt gccggtatgg cgactattct
     1321 gtcccaattg actcgtgacg agcgtttgtt tgcgattgag gcgttgactg ccgaaactcc
     1381 taaaaccaaa gtttttgccg aacaagtgaa aaatctgggt ctggagcaag tgttgtttgt
     1441 aaccaaacag ctcgacgaga atgtttactt ggcttcacgc aacttgccaa acgtgttggt
     1501 tttggaagct caacaagttg atccttacag cttgctgcgt tacaaaaaag taatcatcac
     1561 taaagatgca gttgcacaat tagaggagca atgggtatga atcaacaacg tttgactcaa
     1621 gtgattttgg cacctatcgt ttctgaaaaa agcaacgtat tggctgaaaa acgtaaccaa
     1681 atgacgttta aagttttggc aaatgcaacc aaacctgaaa ttaaagcggc tgttgagctg
     1741 ctgttcggcg ttcaagttgc agacgttact actgttacca ttaaaggtaa agttaaacgt
     1801 tttggtcgca ctttaggtcg tcgcagcgat gttaaaaagg cttatgtaag cttggctgcc
     1861 ggtcaagagt tggatttgga agccgctgct gcagctgcag ataaggaata aacaaaatgg
     1921 caatcgttaa aatgaagccg acctctgcag gccgtcgcgg catggttcgc gtggtaacag
     1981 aaggtttgta caaaggtgca ccttatgcac ctctgctgga aaagaaaaat tctactgccg
     2041 gtcgtaacaa caatggtcat attactaccc gtcataaagg tggtggtcat aaacatcatt
     2101 accgcgtcgt agattttaaa cgtaacaaag acggtatccc tgcaaaagta gagcgtatcg
     2161 aatatgaccc taaccgtact gcatttatcg cactgttgtg ctatgcagat ggtgagcgtc
     2221 gctacattat tgctcctcgt ggtattcaag ccggtgcagt attggtttcc ggtgctgaag
     2281 ctgcgatcaa agtaggtaac actctgccga tccgcaatat tcctgttggt acaactattc
     2341 actgtatcga aatgaaacca ggtaaaggtg cgcaaattgc acgttctgcc ggtgcttctg
     2401 cggtattgct ggctaaagaa ggcgcgtacg ctcaagtccg cctgcgctct ggcgaagtcc
     2461 gtaaaatcaa cgtagattgc cgtgcaacca tcggtgaagt cggtaacgaa gagcaaagcc
     2521 tgaaaaaaat cggtaaagcc ggtgccaatc gttggcgcgg tattcgtccg actgtacgtg
     2581 gtgttgtcat gaaccctgtc gatcacccgc atggtggtgg tgaaggccgt acgggcgagg
     2641 cccgcgaacc ggtcagccca tggggtactc ctgctaaagg ctaccgcact cgtaataaca
     2701 aacgcacgga taacatgatt gttcgtcgcc gttactcaaa taaaggttaa tttagtatgg
     2761 ctcgttcatt gaaaaaaggc ccatatgtag acctgcattt gctgaaaaaa gtagatgctg
     2821 ctcgcgcaag caacgacaaa cgcccgatta aaacctggtc tcgtcgttct accattctgc
     2881 ctgattttat cggtctgacc attgctgtgc acaacggccg cacccatgtg cctgtgttta
     2941 tcagcgacaa tatggttggt cataaattag gcgaattctc attgacccgt acctttaaag
     3001 gccacttggc cgataaaaag gctaaaaaga aataaggtga atcatgagag taaatgcaca
     3061 acataaaaat gcccgtatct ctgctcaaaa ggctcgtttg gtagctgatt tgattcgtgg
     3121 taaagacgtt gcccaagctt tgaatatttt ggctttcagt cctaaaaaag gtgccgagct
     3181 gattaaaaaa gtattggagt cagctattgc taatgccgag cacaataacg gtgcggacat
     3241 tgatgaactg aaagtggtaa ctatctttgt tgacaaaggc ccaagcttga aacgttttca
     3301 agctcgcgcc aaaggtcgcg gtaaccgcat cgaaaaacaa acttgtcata tcaatgtgac
     3361 agtgggtaac taaggaaaag ctatgggaca aaagattaac cctacaggct ttcgcctggc
     3421 ggtaactaaa gactgggctt caaaatggtt tgctaaaagc accgactttt ctactgtttt
     3481 gaagcaggat atcgatgttc gcaattattt gcgtcaaaaa ttggccaatg cttcggttgg
     3541 tcgagtggtt attgaacgcc ctgcaaaatc tgcacgcatt accattcact ccgctcgtcc
     3601 gggtgtggtt atcggtaaaa aaggtgagga tatcgaggtt ttgaaacgtg acttgcaagt
     3661 cttgatgggt gtacctgttc atgtaaatat tgaagagatt cgccgtcctg agttggatgc
     3721 tcaaattatt gctgacggta ttgcccagca gttggaaaag cgcgttcaat tccgtcgtgc
     3781 tatgaaacga gcaatgcaaa atgcaatgcg ttctggtgct aaaggcatta agattatgac
     3841 ttcaggccgt ctgaatggtg cggatattgc ccgtagcgaa tggtatcgtg aaggtcgcgt
     3901 gccactgcat actttacgtg caaatgtaga ttatgcaacc agcgaagcgc acaccacata
     3961 tggtgtattg ggtctgaaag tttgggttta tacggaaggc aatattaaat cttccaaacc
     4021 tgaacatgag agtaaacaaa gaaaggcagg tagacgtaat gctgcagcca actagactga
     4081 aataccgtaa gcaacaaaag ggtcgcaata ccggcatcgc tactcgcggt aataaggtaa
     4141 gtttcggtga gttcggcttg aaagccgtag gtcgtggtcg tttgactgcc cgtcaaatcg
     4201 aagctgctcg tcgtgcaatg acccgtcata tcaaacgtgg tggtcgtatt tggattcgtg
     4261 tattccctga taaaccgatt actgaaaagc ctattcaagt tcgtatgggt ggcggtaaag
     4321 gtaacgtgga atattacatt gccgaaatta aaccaggtaa agtgttgtat gaaatggatg
     4381 gcgttccaga ggaactggct cgtgaagcat tcgagttggc tgctgccaaa ttgcctattc
     4441 ctacaacctt tgtagtaaga caggtgggtc aataatgaaa gcaaatgaat tgaaagacaa
     4501 atccgttgag cagttgaatg cagatttgtt ggacttgttg aaagctcagt ttggcttacg
     4561 tatgcaaaac gctaccggtc aattaggcaa accaagtgaa ttgaaacgtg tacgtcgcga
     4621 tattgctcgt attaaaaccg ttttaactga aaaaggtgct aagtaatgag cgaaactaaa
     4681 aatgttcgta ctttgcaagg caaagtagta agcgacaaaa tggataaaac cgtaacagta
     4741 ttggttgagc gtaaagtaaa acatccgctg tatggtaaga ttattcgatt atctactaaa
     4801 atccatgccc atgatgaaaa taatcaatat ggaattggtg atgtggttgt tatatcggaa
     4861 tcccgtccat tgtcaaaaac taaatcttgg gttgtcagtg agctggttga gaaagcacgt
     4921 tctatttaag aattaaagca acgtgcttgg aatgggaaac gaagtattgc agcaaattta
     4981 atttgcgtgt aaacttcgtt tcctgtcttt cagtttcttc tggaagtttc ttccctttcg
     5041 gggtccaaga ctggtttact tgaaccgcaa ggtttcattt aataagcagc ggctttgctg
     5101 taagttatct gaaagtggta aattaagttg gttaatttaa aggtaataac atgattcaaa
     5161 tgcagaccat cttagatgtg gctgataact ctggtgcgcg tcgcgtaatg tgtatcaagg
     5221 tattgggcgg atctaagcgt cgctacgctt ctgttggcga tattattaaa gtggcagtta
     5281 aagatgcggc tccgcgtggc cgtgtcaaaa aaggcgatgt atataatgcg gtagttgttc
     5341 gtactgctaa gggtgtacgt cgtcctgatg gtgcgttaat taaattcgat aacaatgccg
     5401 ccgtgttact gaataataaa cttgaacctt tgggtactcg tatctttggt ccggtaaccc
     5461 gtgaattgcg tactgagcga tttatgaaaa tcgtttcatt ggcacctgaa gtattataag
     5521 gaatggcacg atgaataaaa tcattaaagg cgatagggtt gtagtaattg ctggtaagga
     5581 taaaggtaag cagggtcaag tagttcgagt gttgggtgat aaagttgttg ttgagggcgt
     5641 taatgttgta aaacgccatc aaaaacctaa tccaatgcgt ggcattgagg gcggtattat
     5701 tactaaagaa atgcctttgg atatttctaa tatcgcaatc ctgaatccgg aaactaataa
     5761 agcggaccgt gttggtatta agctgattga aaatgaaggc aaagttaaac gcgttcgttt
     5821 cttcaaatca aatggctcta tcattggggc ataaggagat aacatggctc ggttgagaga
     5881 gttttataaa gagacagttg ttcctgaatt ggttaaacaa tttggttaca aatcagtaat
     5941 ggaagtcccg cgtattgaaa aaattacctt gaatatgggt gtgggtgagg ctgttgctga
     6001 taaaaaagtt atggaacatg ctgtttccga tttagagaaa attgccggtc aaaaaccggt
     6061 tgttactgtt gcccgtaaat ctatcgcagg ttttaaaatc cgtgataact atccggttgg
     6121 ttgcaaagta acattgcgtc gtgatcaaat gtttgaattc ttggatcgtt tgattactat
     6181 tgcattacct cgcgtacgtg acttccgtgg tgtgagcggt aaatcatttg atggccgtgg
     6241 caattacaat atgggtgttc gtgagcaaat tatttttccg gaaattgaat acgataaaat
     6301 tgatgctttg cgtggtttga atattactat tactactaca gcaaaaaccg atgaggaagc
     6361 gaaagcttta ttgtcattgt ttaaatttcc gttcaaagga taatcatggc taagaaagca
     6421 cttattaatc gtgatctgaa acgtcaagct ttggctaaaa aatatgcggc taaacgcgcg
     6481 gcaattaaag cggtaatcaa tgattcgaat gcaactgagg aagagcgttt tgaggctcgt
     6541 ttgaggtttc aatccattcc tcgtaatgcg gcacctgtgc gtcaacgtcg tcgttgtgct
     6601 ttgacaggtc gccctcgtgg tactttccgt aaatttggtt tgggtcgtat taaaatccgt
     6661 gaaatcgcca tgcgtggcga aattccgggt gttgttaaag ccagctggta ataggagtaa
     6721 ttaagaatga gtatgcatga tcctatttcc gatatgttga ctcgtatccg caatgcgcaa
     6781 cgtgctaata aagcagcggt tgcaatgcct tcttcaaaat taaagtgtgc tattgcaaag
     6841 gtattgaaag aagaaggata tattgaggac ttcgcagttt catctgacgt aaagtctata
     6901 ttggaaattc aattaaaata ctatgcaggt cgtcctgtaa ttgaacaaat caagcgtgta
     6961 tctcgccccg gtttgcgtat ttataaagcg tctagtgaga ttccaagtgt tatgaatggc
     7021 ttgggtattg ctattgttag tacttctaaa ggtgtaatga ctgatcgtaa agcacgttct
     7081 caaggtgttg gtggtgagtt gttatgcatt gtagcctagt ggaggaaaag aaatgtcacg
     7141 tgtcgcaaaa aacccagtga ctgttcccgc tggtgtagaa gtaaaatttg gagcagaggc
     7201 attagttatt aagggtaaga acggtgaatt gtcttttcct ttgcattctg atgtagccat
     7261 tgaatttaat gatggcaaat tgacttttgt tgcgaataac agcagtaaac aagcaaatgc
     7321 aatgtctggt actgctcgcg cattagtcag caatatggtt aaaggtgttt cagaaggttt
     7381 tgagaaaaga ttgcaattga taggtgtggg ttatcgtgct caagcacaag gtaaaatctt
     7441 gaatctgtct ttgggttttt ctcatccgat cgtatatgaa atgcctgaag gtgtctccgt
     7501 tcaaactcct agccaaacag agattgtttt aaccggctcg gataaacaag ttgttggtca
     7561 agttgctgct gagattcgtg cgttccgtgc tcctgagcct tataaaggta aaggtgttcg
     7621 ctatgtagga gaagtagtgg taatgaaaga agccaagaaa aaataattga ggttcactaa
     7681 tggataaaca tacaacccga ctccgtcgtg cacgcaaaac ccgtgctcgt attgcggact
     7741 tgaaaatggt aagattatgt gtgttccgaa gcaataatca tatttatgct caagtaatta
     7801 gtgctgaagg tgataaagta ttggctcaag cctctacatt ggaagctgag gtgcgcggta
     7861 gtctgaaatc tggaagcaat gttgaagcag ctgcaatagt tggtaaacgt atcgctgaaa
     7921 aagctaaagc agcaggtgta gaaaaggttg cttttgatcg ttcaggtttc caatatcacg
     7981 gtcgtgtgaa ggctttggct gaagctgctc gtgaaaatgg tttaagcttc taaatatttg
     8041 gagactttca gatggcaaaa catgaaattg aagaacgcgg tgacggtctg attgaaaaga
     8101 tggtcgctgt taatcgcgta actaaagtag ttaaaggtgg ccgtatcatg gctttctcag
     8161 cactgactgt tgttggtgat ggtgatggtc gcattggtat gggcaaaggt aaatcaaaag
     8221 aagtaccagt tgctgttcaa aaagcaatgg atcaagctcg acgctctatg attaaagtac
     8281 ctttgaaaaa cggtactatt catcatgagg ttattggccg tcatggtgct actaaagtat
     8341 ttatgcagcc tgctaaagag ggtagtggcg taaaagccgg tggacctatg cgtttggttt
     8401 ttgatgctat gggcattcat aatatctccg ccaaagtgca cggatctact aacccatata
     8461 atatcgtacg tgcaacatta gatggtttgt ctaagttgca tactcctgct gatatcgcag
     8521 ccaaacgtgg cttgacagtg gaagacattt tgggagttaa ccatggctga acaaaaaaag
     8581 attagggtta cattggttaa aagcctgatt ggtacaattg aatctcatcg tgcatgtgca
     8641 cgcggtttag gtttgtgtcg tcgcgagcat acggtagagg ttttagatac ccctgaaaac
     8701 cgtggtatga ttaataaaat cagctacttg ttgaaagtgg agtcttgata tgtttttgaa
     8761 tacaattcaa cctgctgttg gtgctacgca tgctggtcgt cgtgttggac gcggtattgg
     8821 tagtggtctt ggcaaaacgg gtggtcgtgg tcataaaggt caaaagagcc ggtctggtgg
     8881 gtttcataag gtgggtttcg agggtggtca aatgcccttg caacgacgcc tccctaaaag
     8941 aggttttaaa tctttaacag catcagctaa tgcacagctt cgtttaagtg aactggaatc
     9001 aattgctgtt aatgagattg atattttggt cttaaagcaa gcgggtctga ttgcatctac
     9061 agtctctaat gttaaagtta ttgcttctgg tgaaatttct aaggcagttg ctttgaaggg
     9121 tattaaagtt accaaaggtg cgagagctgc tatcgaggct gttggtggta agattgaaat
     9181 gtaaggttta atattgtggc taatcaacaa acgtcatcag gttcatccaa atttggagat
     9241 cttaagaaac gtcttttgtt tctatttgga gcattgattg tttttcgaat tggtgcccat
     9301 atacccgtac ctggagttga tgctgttgct ttagctaaat tatacgaaag cgctggaaac
     9361 ggcatcctgg gaatattgaa tatgttttcc ggtgggtcgt tagagcgctt tagtatattt
     9421 gcaataggaa ttatgccata tatttcagct tctattattg tacagctcgc ttctgaaatt
     9481 ttgccatcat tgaaggcttt aaaaaaagaa ggggaggctg gtagaaaggt aattacgaaa
     9541 tatactaggt atggtactgt tttgttagca attcttcaaa gtctaggtgt tgcatctttc
     9601 gtatttcagc aaggaattgt tgtaacaagt tcatttgagt ttcatgtttc cacggtagtt
     9661 tctttggtaa cgggaaccat gtttcttatg tggcttgggg agcaaattac tgaaaggggt
     9721 atcgggaacg gtatttcttt aatcattacg gcaggtattg cttcaggtat tccttcgggt
     9781 attgcaaagc tggttacact gacgaaccaa ggttctatga gcatgcttac ggcgttgttt
     9841 attgtatttg gtgccttatt attaatttat ttggttgtat actttgaaag tgcacagcgg
     9901 aagattccta ttcattatgc aaaacgccag tttaatggta gggcgggtag tcaaaatacg
     9961 catatgcctt tcaagttgaa tatggctggt gttattcccc caatttttgc ttccagtatt
    10021 attctatttc catctactct tttaggttgg tttggttcgg ctgatacaaa tagtgttttg
    10081 cacaaaatag ctggattgtt acaacacggt caattgctgt atatggcttt atttgcagcg
    10141 acagttattt tcttttgtta tttttatacg gctttggttt ttagccctaa agaaatggca
    10201 gagaatttaa aaaagagtgg tgcttttgtt cctgggatta gacctggtga gcagacctct
    10261 aggtatttag aaaaagttgt attacgtttg acattgtttg gagctcttta tattacaact
    10321 atttgtttaa ttccagagtt cttaactacg gttttaaatg taccttttta tttgggtggc
    10381 acgtctttgt tgattctagt tgttgtaacg atggatttta gtacacaaat aaattcgtat
    10441 aggcttactc aacagtatga taagttaatg actcgttcag aaatgaaatc attttctcgg
    10501 aaatagaatt atggcgaaag aagatactat ccaaatgcaa ggtgaaattc ttgaaacttt
    10561 acctaatgca acatttaaag taaaacttga gaatgaccat attgtattgg gtcatatttc
    10621 tgggaagatg cggatgcatt acattcgtat ttctccggga gataaggtca cagtagagct
    10681 gacaccttat gatctaacta gggctcgaat cgttttcaga gcaagataaa ccaataaaag
    10741 gaaaataaaa tgcgtgtaca accatctgtt aagaaaattt gccgaaattg caagattatt
    10801 cgtcgaaatc gtgtagttcg tgtaatttgt actgatctcc gtcacaaaca gcgtcaaggt
    10861 taatggaata tttcttttaa tgtgattctg tgatatagtg acacactttg ccctaaaaag
    10921 gaaaaaatat ggctcgtatt gcaggggtaa atatccctaa taacgcacac atcgtaattg
    10981 gtcttcaggc tatttacggt attggtgcta ctcgtgctaa attgatttgt gaggctgcaa
    11041 atattgcgcc tgatactaaa gcaaaagatt tggacgagac tcaattagat gctttgcgtg
    11101 accaagttgc caagtatgaa gtagaaggtg atttgcgtcg tgaggtaact atgagtatca
    11161 agcgattgat ggacatgggc tgctatcgtg gcttccgtca tcgtcgcggc ttaccatgcc
    11221 gcggtcaacg cactcgtaca aatgcgcgta cccgcaaagg tccgcgtaaa gcgattgctg
    11281 gtaagaaata aattttaagg aattttatta atggctaaag caaacacagc ttcacgtgta
    11341 cgtaaaaaag tacgtaaaac cgtgagtgag ggtattgtgc acgttcatgc atctttcaac
    11401 aataccatca ttacaatcac tgaccgtcaa ggcaatgcgt tgtcttgggc tacctctggc
    11461 ggcgctggtt ttaaaggttc tcgtaaaagt acaccatttg cagcacaagt tgcagcagaa
    11521 gcagctggta aagttgccca agagtatggc gttaaaaatt tagaggttcg tattaaaggt
    11581 ccaggtccag gtcgtgaatc ctctgtacgt gctttgaatg ctcttggttt caagattacc
    11641 agcattactg acgttacccc gttgcctcat aacggttgcc gtccgcctaa aaaacgtcgt
    11701 atttaatatt ggagtgattt gaaacatggc acgttatatt ggccctaaat gtaagttggc
    11761 acgtcgcgaa ggtacggatt tgtttttgaa gagtgcgcgc cgctctttgg attctaaatg
    11821 taaaattgat tccgctcctg gtcagcatgg tgcaaaaaaa ccgcgtttgt cagactatgg
    11881 tttgcagttg cgtgaaaaac aaaaaatccg ccgtatttat ggcgtattag aacgtcagtt
    11941 ccgtcgttat ttcgcagaag ctgatcgtcg taaaggttct accggcgagt tgctgttgca
    12001 gttgctggaa tctcgtttgg ataatgtcgt ttatcgtatg ggtttcggtt ctacccgagc
    12061 tgaagcaaga cagcttgttt ctcataaggc gatagttgtg aatggacaag ttgtcaatat
    12121 tccttctttc caagtgaaag ctggtgatgt tgtctcagtt cgtgaaaaag ccaaaaaaca
    12181 ggtacgtatt caagaagcat tgggtttggc aactcaaatc ggcttgccgg gttgggtttc
    12241 tgtagatgcg gataaacttg agggtgtgtt caaaaacatg ccggatcgct cggaattgac
    12301 cggtgatatt aatgaacagc tggtggtaga gttctactct aaataatgct agctcagtga
    12361 gggacagtta aatgcagaat agcacaaccg aatttttgaa acctcgtcaa attgatgtaa
    12421 atactttttc tgcaactcgt gcaaaagtat ctatgcagcc atttgaacgt ggtttcggtc
    12481 ataccttagg taatgctttg cgccgtatct tactgtcatc catgaatggt tttgctccta
    12541 ctgaagtagc tattgccggt gtattacacg aatattctac tgttgatggt attcaggaag
    12601 atgttgttga cattttgctg aatattaaag gtattgtgtt taaactccat ggtcgtagcc
    12661 aagttcaact tgtgttgaag aaatcaggtt caggtgtcgt atctgccggt gatattgagt
    12721 tgccgcatga tgtagaaatt ctgaatcctg gtcatgtcat ttgtcatttg gctgataacg
    12781 gtcaaattga gatggaaatt aaagtagagc aaggtcgtgg ttatcaatct gtttcaggtc
    12841 gtcaggtagt tcgtgatgag aaccgtcaga ttggtgcaat ccagttggat gcgagctttt
    12901 cgcccatcag ccgtgttagc tttgaggttg aacctgcacg tgtagagcag cggacggatc
    12961 ttgataagtt ggttttggat atcgaaaccg acggttctat tgatcctgag gaagctgtac
    13021 gcagtgcggc acgtattttg attgatcaga tgtctatttt tgctgatttg cagggtacgc
    13081 ctgtggagga ggttgaagaa aaagcacctc ctatcgaccc tgttcttttg cgtccggtgg
    13141 atgatctgga attgacagta cgttcagcta attgtttgaa agctgaggat atttattata
    13201 ttggcgattt gattcaacgc actgaaaccg agcttcttaa aacgccgaat ttgggacgta
    13261 aatctttgaa tgagattaag gaagtattgg catctaaagg tttgacactg ggttctaagt
    13321 tggaagcatg gccacctgta ggcttggaaa agccttaatg aagaattaaa ggataattga
    13381 tatgcgtcat cgtaatggca atcgcaaatt aaaccgtacc agcagtcatc gtgctgcaat
    13441 gctgcgtaat atggcgaatt cattattgac tcacgaagct attgtaacaa ctctgcctaa
    13501 ggccaaggaa ttgcgccgtg tagtagagcc gttgattaca ttgggtaaaa agccgtcatt
    13561 ggcaaaccgc cgtttggcat ttgaccgtac tcgcgaccgt gatgttgtag taaaactgtt
    13621 tggcgatttg ggtcctcgtt ttactgctcg taacggtggt tatgttcggg tgttgaaata
    13681 cggattccgt aaaggtgata atgcacctct ggcactggtt gaattggttg acaaaccggc
    13741 tgctgagtaa ttttagtcat ataacgccat ctgccgaaaa gcaggtggcg ttatttttgc
    13801 aatatctgat aggtaatagg gtattggcta tcatgtttaa aatattaatt gaatagctaa
    13861 ggtttgcgcg gtaaacttac atcattaaaa aattctatga tggtttatat aatgaatgct
    13921 ttcgatataa agtcgacaaa gatggacgta ttgtctatat ctttgcatac gtcagacttg
    13981 tttgatttgg aagatgtgct ggtcaaattg ggcaagaagt ttcaagagtc tggtgttgtt
    14041 ccatttgtgc tggatgttca agagtttgat tatcccgagt ctttggatct tgctgcattg
    14101 gtttcgttgt tttcaaggca tggtatgcaa attttgggtc tgaagcattc taatgaacgt
    14161 tgggctgctg cggctatgaa gtatcatttg ctgttttgtc tgtctcattc ggaaaatgtt
    14221 aaagaactgg gtcaggttga ggtgcagaaa acggaggatg gtcagaaagc aaggaaaaca
    14281 gtattgatta catcccctgt ccgtaccggt cagcaggttt atgccgaaga tggcgatttg
    14341 attgttacgg gggcggtcag ccagggggcg gaattgattg cggatggcaa tatacatatt
    14401 tatgcgccga tgagggggcg tgctttggcc ggtgccaagg gtgatacttc tgcccgcata
    14461 tttatccact ccatgcaggc agaactggtt tctgtggcgg gtatttaccg taattttgaa
    14521 caggatttgc cgaaccatct gcacaagcag ccggtacaga tattgttgca ggataaccga
    14581 ttggttatca gtgcaattgg ctcagagtaa ttgtttgata tttaaaaagg aaatattgtg
    14641 gcaaaaatta ttgtagtaac ttcaggtaag ggcggtgtcg gtaaaacgac taccagtgcc
    14701 agtattgcga caggtttggc attacgcgga tataaaactg cggtaattga ttttgatgtg
    14761 ggtttgcgta acctcgacct cattatgggt tgcgagcgtc gtgtcgttta tgacctgatc
    14821 aatgtcattc agggggaggc gacgctcaac caagctttga ttaaagataa aaattgtgaa
    14881 aacctgttta ttttgccggc ttcccagact cgggataaag acgctttgac acgcgagggc
    14941 gtagaaaaag tgatgcagga gctgtccggc aagaaaatgg gctttgagta tattatttgc
    15001 gactctcctg ccggtattga gcagggtgca ttgatggcgt tgtattttgc tgatgaagcc
    15061 attgtaacga ccaatcctga ggtttccagt gtgcgtgact ccgacaggat tttgggaatt
    15121 ttgcaaagca aatcccataa ggcagagcaa ggcggttcgg ttaaagaaca tctgttgatt
    15181 acgcgttatt ctcccgaacg tgtggcaaaa ggcgaaatgc tgtctgtaca ggatatttgc
    15241 gatattctgc atattccttt gctgggtgtg attcctgaat cccaaaacgt cttgcaggca
    15301 tccaattccg gagaaccggt catccatcag gacagcgtgg cggcttccga ggcatataag
    15361 gacgttattg cccgtctttt gggcgagaac cgtgaaatgc gtttcttgga agctgagaaa
    15421 aaaagcttct tcaaacgtct gtttggagga taaggtatgt cattaatcga atttttattc
    15481 ggcagaaagc agaaaacggc aaccgttgcc cgcgaccgcc ttcaaatcat cattgcccaa
    15541 gagcgcgccc aagaaggtca ggctccggat tacctgccga ctttacgtaa agagttgatg
    15601 gaagtcctgt ccaaatatgt gaatgtttca ttagacaata tccgtatttc ccaagaaaag
    15661 caggatggta tggatgtgct tgagttgaac attactttgc cggaacagaa aaaggtatag
    15721 gacatgacct taaccgaatt gcggtacatc gtcgcagtcg cccaagaacg tcatttcggc
    15781 agggcggcgc ggcgttgttt tgtcagccag cccactttgt ctattgccat taagaaattg
    15841 gaagaagagc ttgccgtctc tttgtttgac cggagcagta acgatattat tacgaccgag
    15901 gcgggggaac gtatcgttgc acaggcgcgt aaggtattgg aagaggcgga gcttatcagg
    15961 catttggcaa atgaagaaca aaacgagctg gagggtgcgt tcaaactcgg gctgattttt
    16021 acggttgcgc cgtacctgct gccgaaactg attgtttcgt tgcgccgtac tgcaccgaaa
    16081 atgcctttga tgttggaaga gaattacacg catactttga ccgagtcgct caaacgcggg
    16141 gacgttgatg cgattatcgt tgccgaaccg tttcaagagc cgggcattgt taccgaaccc
    16201 ttgtatgacg aaccgttttt cgtgattgtc ccgaaagggc attcatttga ggaactggat
    16261 gccgtttcgc cccggatgct gggtgaggag caggttttgc tgctgacgga aggcaactgt
    16321 atgcgggatc aggtactctc aagctgttcc gaattggcgg cgaaacaacg tatacagggg
    16381 ttgaccaata cattgcaggg cagctcgatt aatacaatcc gccatatggt tgccagcggt
    16441 ttggcaatca gcgtgttgcc ggcaaccgca ctgaccgaaa acgatcatat gctgttcagc
    16501 attattccgt ttgagggtac gccgccaagc cggcgggtcg tattggcgta ccgccgcaat
    16561 tttgtccgtc cgaaggcgtt gtcggcgatg aaggcggcga ttatgcagtc gcagcttcac
    16621 ggggtaagtt ttatctgcga ctaggcgcag gcattgtttt caaaacgcca tttccctgag
    16681 ccgacaacac ggtatgccaa gatattgccg tcatcatcga ttttgagtat agcatcgcca
    16741 cggaaactgc cgtcctgaag atattcgact tttgcatcac tgtgaatgtt ttcatcagtg
    16801 ccgatgcaat gcca
//
LOCUS       DAWGRZ010000027        16612 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000027 DAWGRZ010000000
VERSION     DAWGRZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16612
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.027"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(123..458)
                     /locus_tag="WHT44_000621"
     CDS             complement(123..458)
                     /locus_tag="WHT44_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ2532842.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(538..2652)
                     /gene="dnaX"
                     /locus_tag="WHT44_000622"
     CDS             complement(538..2652)
                     /gene="dnaX"
                     /locus_tag="WHT44_000622"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ2532843.1"
                     /translation="MAYQVLARKWRPKTFADLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYEPAALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPNALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELKSPSAQTAEKETAAK
                     KPQPRPEAETAQTPVQTASAAAMPSEGKTAEPVTNQENNDIPPWEDAPDETAAGTAQA
                     SAKSIQTASEAGTPPKNQVSKNEAADNETDAPLSEVPSENPIQATPNNEALETEAFAH
                     EAPAKPFNGYSFPNDDYLVEDGAEIPPPDWEHAAPADAEEENNADESSNNEDHTPYAP
                     PPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARATADKKR
                     LDKIRDTLAQAYGLQLTLQTQDWRDEAGRETPAMQDKRVQAEDRQKAQALLEADPAAQ
                     KILQAFGAQWQPESLELAANRP"
     misc_feature    complement(1424..1463)
                     /gene="dnaX"
                     /locus_tag="WHT44_000622"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            complement(2747..4723)
                     /gene="mutL"
                     /locus_tag="WHT44_000623"
     CDS             complement(2747..4723)
                     /gene="mutL"
                     /locus_tag="WHT44_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ2532844.1"
                     /translation="MSRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAIE
                     VELAGGGIRLIRVSDNGGGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQNDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGEDFQT
                     ASLGIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRANLTE
                     SVGNAGEVLHDITGVVSTPMPSENDSENLFDSVSNYPTGNKSDTHNAFGSSGKTAPMP
                     YQSAYAPQQRSLSLRESRAAMNTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGNLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAVPAML
                     GKADVVSLAKDVLNELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            4867..5052
                     /locus_tag="WHT44_000624"
     CDS             4867..5052
                     /locus_tag="WHT44_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ2532845.1"
                     /translation="MYHYQSDATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            5063..5731
                     /locus_tag="WHT44_000625"
     CDS             5063..5731
                     /locus_tag="WHT44_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ2532846.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKTGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDPRIVPITLPVGDGLTL
                     LLKK"
     gene            5731..6444
                     /locus_tag="WHT44_000626"
     CDS             5731..6444
                     /locus_tag="WHT44_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ2532847.1"
                     /translation="MKIKLPLFIIWLSVSASCASVSPVPAGSQTEMSTRENASDGIPY
                     PVPTLQDRLDYLEGKIVRLSNEVETLNGKVKALEHAKTHSSGRAYVQKLDDRKLKEHY
                     LNTEGGSASAHTVETAQNLYNQALKHYKSGKFSAAASLLKGADGGDGGSIAQRSMYLL
                     LQSRARMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWRSLI
                     QTYPGSPAAKRAAAAVRKR"
     gene            6492..6977
                     /gene="purE"
                     /locus_tag="WHT44_000627"
     CDS             6492..6977
                     /gene="purE"
                     /locus_tag="WHT44_000627"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ2532848.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFSIGEAGAANAALFAVSMLANETPELAQKLADFRAKQEQAVLNMELEQ
                     I"
     gene            complement(7160..8614)
                     /locus_tag="WHT44_000628"
     CDS             complement(7160..8614)
                     /locus_tag="WHT44_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ2532849.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPTEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(8611..9312)
                     /locus_tag="WHT44_000629"
     CDS             complement(8611..9312)
                     /locus_tag="WHT44_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ2532850.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEVERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            complement(9309..10088)
                     /locus_tag="WHT44_000630"
     CDS             complement(9309..10088)
                     /locus_tag="WHT44_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ2532851.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            10373..11779
                     /locus_tag="WHT44_000631"
     CDS             10373..11779
                     /locus_tag="WHT44_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ2532852.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADL
                     NESPLDMQLAVISYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQIAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWVFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVAGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGTNLTRNTPS"
     gene            11891..13417
                     /locus_tag="WHT44_000632"
     CDS             11891..13417
                     /locus_tag="WHT44_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ2532853.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGDIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     CIDWQSVRTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPAYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
     gene            13625..14158
                     /locus_tag="WHT44_000633"
     CDS             13625..14158
                     /locus_tag="WHT44_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ2532854.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
     gene            complement(14201..15502)
                     /gene="aroA"
                     /locus_tag="WHT44_000634"
     CDS             complement(14201..15502)
                     /gene="aroA"
                     /locus_tag="WHT44_000634"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ2532855.1"
                     /translation="MTESVRLPVARLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVQIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLVDALRIAGADVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQSFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETAE"
     gene            15898..16374
                     /gene="greA"
                     /locus_tag="WHT44_000635"
     CDS             15898..16374
                     /gene="greA"
                     /locus_tag="WHT44_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ2532856.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VIYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
BASE COUNT         3491 a         4481 c         4637 g         4003 t
ORIGIN      
        1 tgatgccgtc attcccgcga aggcgggaat ccagacccct cggcacggaa atttatcgag
       61 taaaacggtt tcttagattc tacgttctag attcccgcct gcgtgggaat gacggtcggg
      121 gatcagcgga agaagtcgcc cactccgggg ggtagacctt gcgtgaatgc gcccattgtt
      181 ttgtttgcgg tttcttcggc tttgcctcgg gcggatttga gggcggcgag gatgaggtct
      241 tcaagcattt ctttgtcgtc ggcggcttct tgaatcaaat cggggctgat gtcgattttg
      301 cgtacttcgt gcgcgcaggt cattgtgatt ttgaccaggc cgttgcctgc ttcgccttcg
      361 atttcggttt cggcgagttt ggcttgcgct tttttcatat tttcctgcat ttgctgcgcc
      421 tgtttcatca ggccgcctaa tccggctttt ccgaacatac tgaatactcc tgtctgtttt
      481 gattgaataa gggaaacggc aatgccgtcc gaagggttcg ggcggcatta tatctgttta
      541 tggccggttt gccgccaatt ccagtgattc gggctgccat tgcgcgccga atgcttggag
      601 gattttttgt gcggcggggt cggcttcgag caatgcttgt gctttttgcc tgtcttcggc
      661 ttggacgcgc ttgtcctgca tcgcgggggt ttcccggccg gcttcgtcac gccagtcttg
      721 ggtttgcagg gtgagttgca gcccgtaggc ttgggcaagg gtgtcgcgga ttttgtcgag
      781 gcgttttttg tcggcggtgg cgcgtgcttc ggcggtcatt gccaaaacca tcagaccggt
      841 gtcgggatgg tattccgtcc acgcggagtg ttgcgccggc atttgcgccg cgccgagttt
      901 gcgggcgaag tgccggacga tggctgccca gttttcggtg gaaaattcgg gcggcggggc
      961 gtatggcgtg tggtcttcgt tgttgctgct ttcgtcggcg ttgttttctt cttccgcatc
     1021 ggcaggggcg gcgtgttccc aatcgggcgg tgggatttct gcgccgtctt ctacgaggta
     1081 gtcatcattc ggaaagctgt aaccgttgaa aggttttgca ggagcttcgt gtgcaaatgc
     1141 ttctgtttca agggcttcat tattcggtgt tgcctgaatg gggttttcag acggcacttc
     1201 ggacaagggg gcatcggttt cgttgtcggc tgcttcgttc ttggaaactt ggtttttggg
     1261 cggcgttccg gcttcggatg ccgtctgaat gctttttgcc gatgcttgcg ccgtgccggc
     1321 tgcggtttcg tccggcgcgt cttcccaagg cggaatatcg ttgttttctt gattggtaac
     1381 gggttcggca gttttgcctt cagacggcat tgctgctgcg gatgccgtct gaacgggtgt
     1441 ttgggcggtt tccgcttcag ggcgcggttg gggctttttt gcggcggttt ccttttcggc
     1501 ggtttgtgcc gatggggatt ttagttcggt attttcaatc acggcatttg catcacacga
     1561 tgctgccgcc aagggcgcaa acgccagcat acgcagcagg gtcatcataa agccggcgta
     1621 ttcgtcgggg gcgaggctga ggtcgcgttt gccgtggacg gcgatttggt agtaaagctg
     1681 gatttgttcg ccgcttatgg tttgggcgag gcggtgcaaa atatcggaat cggggtcgtc
     1741 gtgcgccaag gcattcggca ctgcctgtat cagggcgagg tgttgcagca gtatggcaag
     1801 ttcgcccaag gcgttgtcaa agccgacggc acacgccgcc atttcctgcg ctttggcggt
     1861 cagggctgcg ccgtcttggt tgatgatgcc tgtcagcagt tcgtaaaggt attgtttgtc
     1921 aaccgcgccg atcatttggc ggacatcgtt ttcggcaact ttgcccgaac ctagggcgat
     1981 ggcttggtcg agcaggctca aggcatcgcg catcgatccg gcggcggcac gtcccaaaag
     2041 ttgcagggcg gcgggttcgt aggcgatttt ttcgctgtcg aggacgtggg cgaggtggtc
     2101 ggcaacctgt tgcgcggtca tattgcgtaa gacgaattgc aggcagcggc tcaagacggt
     2161 aacgggaact ttgtgcggat cggtggtggc gaggatgaat ttgacgtgtt cgggcggctc
     2221 ttccagcgtt ttgagcatag cgttgaacgc gcttttggaa agcatatgca cttcgtcgat
     2281 gatatagact ttgtattttc cggcggtcgg tgcatattgg gcgttttcca agacttcgcg
     2341 gatgttgtcg atgcctgtgt tggaggcggc gtcgatttcc agcaggtcga cgtagcgtcc
     2401 ggcatcgatc tgcgtacagc tttcacatac gccgcaaggt tcgccgtgtt gcgcgttttc
     2461 gcagttgagg cttttggcaa ggatgcgggc gatggtggtt ttacctacgc cgcgcgtgcc
     2521 ggtcagcagg taggcgtggt gcagcctgcc ttcgtccagg gcgttttgca gggctttgac
     2581 gacgtgttcc tgaccgacta agtcggcaaa ggttttgggc cgccattttc gggcgagaac
     2641 ttgataggcc atgtttttct cttggtttcg gtcgtgatgt ttctgtcggt gcgtcggaat
     2701 gccgtctgaa cggcggtctc gggcggcgta ttctagcact ttcggcttac tgtccgcgca
     2761 agaacagtgc gtccaattct ttcaaagtca gtttgaccca agtcggcctg ccgtggttgc
     2821 actggttgct gcgcggcgta ttttccatat cgcgcagaag ggcgttcatt tcgggcaggg
     2881 tgagccggcg gccggcgcgg atcgagccgt ggcaggacat ggtggcgagg atgcggtttt
     2941 cgtgttcctc gatggtttgg ctgctgccga cttgggcgag ttcgtttaat acgtctttgg
     3001 cgagcgagac gacatcggct ttgccgagca tggcgggaac tgcacggacg gcgagggtgt
     3061 tgccgcccat atcggataat tccagcccga agcctgccag cgtttcggca taatcggcaa
     3121 gggcggcgca ttcttcgtgg gacgcggcaa aggttacggg aataagcagg cgttggcttt
     3181 gcaggttgcc gttttcctga cgttggcgtt tcattttttc gtagttgacg cgttcggcgg
     3241 cggcgtgcat atcgatgagc aacaggctgt cttcggcttg ggcaagaatg tagatgccaa
     3301 gtaattgggc aatggcaaaa ccgagcggcg gcagttcgga ttgggacggg atgccgtctg
     3361 aaagcggcgt atctgtttgg ggagcaggcg tttcagacgg catattgccg aaacgtgcct
     3421 gctcgaatcg gcttaactca aggtcgatgt cgtcggtttt tttgtaaagt tcggcgtaag
     3481 tattcattgc cgcgcggctt tcgcgcaggg acaggctgcg ttgttgcggc gcatatgcgg
     3541 actgataggg catgggcgcg gttttgcctg atgaaccaaa ggcattgtgt gtatctgatt
     3601 tgttgcctgt cgggtagttg gatacgctat caaacagatt ttcgctgtcg ttttcagacg
     3661 gcattggggt ggagacaacg ccggtaatgt catgcaacac ttcgcctgcg ttgccgacgc
     3721 tttcggtcag gttggcgcgt gtgtcggcaa gggctttgtt gagcgtgtgg aacacaagtt
     3781 ggtgcacctg ctgactgtcg cggaagcgga tttcggtttt ggtcgggtgg acgttgacat
     3841 ccacggcttc gggcggcagg tcgagaaaga ggacgaaggc gggagtgagt gcgttgtgca
     3901 atacgtcgcg gtatgcctgc ttgacggcgt ggagcatcac tttgtcgcgc acgaagcgat
     3961 ggttgacgaa gcagtattgt ttgtcggttt tacctttggc gaaagtcggt ttggcaatcg
     4021 caccatagag ccgcagcgcg ccgttgccgc tgtcgattcc caatgatgcc gtctgaaagt
     4081 cttcgccgac aatggcggca atccgttcat gcaggctttg tgcagggagt ttgaacactt
     4141 gtttgccgtc gcgtttgagc gagaaggcaa tgtgcggatg cgccagcgcg aggcgttcga
     4201 gcatggtggc gcagtgggcg tattcggtgt tttcggattt gaggaacttg cgccgtgcgg
     4261 gggtgttgaa gaagagttcg gcggcttcga tggtggtgcc gacggggtgg gcggcggcgg
     4321 tggggctgct gagtttgccg tcttcggctt tgacttgggt cgcgtgcgaa ctgtcgttct
     4381 gacggctggt cagggtcagg cggctgacgg aggcgatgct tgccaaacct tcgccgcgaa
     4441 agcccatact ggcgacgtgt tccaaatcgt ttaaggtttt gattttgctg gtggcgtggc
     4501 ggtggagcgc aagttcgatg tcgtcggggt ggatgccgcc gccgttgtcg ctgacgcgaa
     4561 tcaggcggat gccgccgccc gccagctcga cttcaatcgc cgttgcgcct gcatcgatac
     4621 tgttttcaac gatttctttc aaggcgttgg cagggcgttc gaccacttcg ccggcggcga
     4681 tttggttgac aagatggtcg ggcagggcgg cgattcggga cataaggcgg gcttccgttg
     4741 caaaaaacgt cttattctat aataaaaccc cttatctttc tgcccgtatt cttgataaaa
     4801 gccgtttatc ccgtttggaa aataccagta taatcacgcc atattccgta aaattggagc
     4861 acaaagatgt atcactacca atccgatgcc acacaattcc tcaaccgcct gattgaagaa
     4921 aaacctgagt tggaacagca gcgtttggaa aacagggggc ttttgtggga tgtcgaactc
     4981 aatcccgaag aacaggaaaa cttcgaggcg gcaaaagtgg cgaaaaaacc ttatacctat
     5041 taccaagact gaatccgccg aaatgacgac ccatctgtcc aatgtcgcac cggacctgca
     5101 aaactatttg aacgccatcg gcgaacccga acatcccgtt ttgacgcggc tgcgcgagaa
     5161 gaccgggcat caccgtatgg gcaaaatggc gattgcgcgc gaacaggcgg cagttttggt
     5221 ttggctggca aagctgatcc gtgcggaaaa atatctggaa atcggcgtat ttaccggata
     5281 cagcagcacc gcgcttgcat tggcactgcc cgaacacggg cggattaccg cctgcgacat
     5341 caatgtaacc tttaccgata cggcgcgtca ggtttggaac gaggccggtg tggcacataa
     5401 aatcagcctg cacctgcaac ccgcattgct gacattggat gatttgattg cacagggtga
     5461 agccggaagc tacgatttgg cactgataga cgcagacaaa ccgcccacgc cgcaatattt
     5521 cgagcgttgc ctcaaactcg tccgtcaagg cggcatcatc gccatcgaca atattttgct
     5581 gaacggaagg gtgatgcgcg aagcggcttc cgatgcgccg cccagcgtcg gcatcctcaa
     5641 agatttcaat caaaacctgc cgaacgaccc gcgcatcgtc cccatcaccc tgcccgtcgg
     5701 cgacggcttg accctgcttc tgaaaaaata atgaagatca aattaccgct ttttatcatt
     5761 tggctgtctg tgtccgcctc ctgtgcttcc gtttcacccg ttccggcagg cagccaaacc
     5821 gaaatgtcga cacgggaaaa tgcttcagac ggcattccct atcccgttcc gaccttgcaa
     5881 gaccgtttgg actatctgga aggcaaaatc gtccggctgt cgaacgaagt ggaaacctta
     5941 aacggcaaag tcaaagcact ggaacacgca aaaacacatt cttccggcag ggcatacgtc
     6001 caaaaactcg acgaccgcaa gttgaaagag cattacctca ataccgaagg cggcagcgca
     6061 tccgcacata ctgtcgaaac cgcacaaaac ctctacaatc aggcactcaa acactataaa
     6121 agcggcaagt tttctgccgc tgcctccctg ttgaaaggcg cggacggagg cgacggcggc
     6181 agcatcgcgc aacgcagtat gtacctgttg ctgcaaagca gggcgcgtat gggcaactgc
     6241 gaatccgtca tcgaaatcgg agggcgttac gccaaccgtt tcaaagacag cccaaccgcg
     6301 cctgaagcca tgttcaaaat cggcgaatgc caatacaggc ttcagcaaaa agacattgca
     6361 agggcgactt ggcgcagcct gatacagacc tatcccggca gcccggcggc aaaacgcgcc
     6421 gccgcagccg tgcgcaaacg atagtttttc catttttccg ctttttaccc catccgcacc
     6481 aaggaggatt tatgatacaa atcggcatca tcatgggcag caacagcgat tggcccgtta
     6541 tgcggcaggc agcgcagttt cttgaagagt tcggcgtaga atatgaggcg cgcgttgttt
     6601 ccgcacaccg caccccggat ttgatgttcc aatacgccga aaccgcacgg gcgcgcggca
     6661 tcaaagccat catcgcgggt gcgggcggcg cggcgcattt acccggtatg gttgccgcca
     6721 agaccaccgt ccccgttttg ggcgtacccg tccccagcaa atacctgcgc ggcgaagatt
     6781 cgcttttatc gattgtacaa atgcccaaag gtgtacccgt cgccacattc tccatcggcg
     6841 aggcaggcgc ggcaaatgcc gcattgttcg ccgtttccat gctcgccaac gaaacccccg
     6901 aactggcgca aaaactggca gacttccgag ccaaacagga acaggctgtt ttgaatatgg
     6961 aattggaaca aatttaaaac ccaccgtctg cgaatatata gtggattaaa tttaaaccag
     7021 tacggcgtta cctcgccttg ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc
     7081 tgatttttgt taatccacta tacaaatgcc gtctgaagcc ctgtttcggg tttcagacgg
     7141 catttttgcg aacaactttt tatgcctgtt ctttttggcg cattttttct gccattagtt
     7201 cgtgcagggt tttcttcgcc ggtttcatcg gcacgcggtt ttgcgtccaa cccaactgtt
     7261 tgcgcggggt caggttgcgg aacttggtgg ctgcccaacc gaaggcgcgg taggttttgc
     7321 tgccgctgaa aataccgttg aatgtgcgcc acgccatttg ttcgccgaag gtatgcgatg
     7381 cgccttgccc ccggatgggg tgcggcacgg tttcggtcgg cgaacgttgc gcttcaacgc
     7441 gcaaacgctg catttgttcg gtaatcggga tgcgtaccgg acaaacttcc acgcacgcgc
     7501 cgcacatcgt gcaggcggtc ggcaggtcgc gagtggcatc caagcctaac aggtgcgggg
     7561 aaataatctc gccaatcgga ccgggatagg ttgtgccgta tgccgcgccg ccgatgcggg
     7621 tataaaccgg gcaatggttc atacacgcgc cgcaacggat acattgcagg gtgcggcgca
     7681 tttggtcttc ggcataagcc tggctgcggc cgttgtcgag cagaaccaag tgcatttctt
     7741 gcggaccgtc taattcttca ctgcggcgcg ggccggtaat catattgaaa taagtggtaa
     7801 tgttctgacc aatggcagaa cgcggcagca ggctgtacaa gggtgggatg tcggacaatt
     7861 tcgccaccac tttttcaatg ccggtaatag cgatatgcac gggcggtacg gtggtactca
     7921 agcgaccgtt gccttcgttt tccaccagac acagcgtacc tgtttcagca acggcaaagt
     7981 ttacgccact caaaccgaca tcggcagtgc tgtaaatatc gcgcagtgct ttacgggcga
     8041 agccggtcag ttggtctaca tcgtctgtca gcggcgtacc gaggttttgg tggaacagtt
     8101 cgctaacctg ttctttggtt ttgtggatag caggcatcac gatatgggtc ggtttttcgc
     8161 ctgccatttg gacgatgaac tcgcccaagt cgctttctac cgctttaatg ccttttgctt
     8221 caagataatg gttcagctcg atttcctcgc tgaccatcga tttgcctttg accatcagct
     8281 tgccgttttt ggctgtgatg atgtcgtgga taatttggca ggcttcggtc ggggtttctg
     8341 cccagtgcac tttcacgccc aacttagtca ggttttcttc cagctgctcc agcagggctg
     8401 gcaatttaga caatgagcgc tgacggacgt gttcgcacaa atcgcgcagg ctttgcagct
     8461 cttcttcgtc ggtcaaaacg gctttgcgtt tggtcatcag catatccatc gcggtacgca
     8521 ggcttttgcg caaaggcttg tcttgaaggg aaattgcggc gttttgcttg aaagtttccg
     8581 gcttcatgtg aaatttgatg gtttgcgtag tcatgcgttt tcctccaaat cggcagggga
     8641 aatgtggtcg ggcaggatgg cgaggatgac caaatcgcgc gggccgtgcg cgccgtaagc
     8701 aagcgtcagt tggatgtctg cggttttgga cgggccggaa atcaggaata cattggtcgg
     8761 cataccgttt tccaccagtt tttcgccttc gacggcatta tgaaactcgt tgtacatctt
     8821 ggacgtatcg aacaggcaga aatgcacggg cggaacgagg cttaaagtac gcggttcttc
     8881 ggggctggaa aacagcatca gcgtgccggt gcgggcgatg ccgcattgcg cgccgctgaa
     8941 gcccgcatcg atgttcgtga aaaactcggt tttccaagta tcgatttcgc gctcgaaggc
     9001 aatcggttcg atattgctgt ccgccaatgc ggcacgggca atttgtccgt gttcggtcgc
     9061 caagggcagc aggatgtttt tcaaaccctt gccttctgcc gcttcgcgga aaacctgcat
     9121 ccaattgctt ttcgtcaccc aataaatttc ggttttgacc gcacgcatag cggcagccca
     9181 atgtttcaga cgctcaactt cgctgcccca agaaacaccc atttcacggt aataatcaaa
     9241 aaccgcaggt tcttccatcg gcaatgcgtc ggcttttttc agttttgcca aaatattttc
     9301 acgcgcgctc atgctttgcc tccggtgcgt tccaacaaga aggatgcgat atgtttcgga
     9361 cgcggcatat ccggctcgtc cttggcgatt ttgccgccga tgttcatcat acagccgcag
     9421 tccgcgctga tgatttcggt tgcgccggtt tctttcagcg cggcgacttt gtctgttacc
     9481 attgcgccgg aaatatcggc ttgtttgacg gagaatgtgc cgccgaagcc gcaacattcg
     9541 ctttcgtggt cgtggacgat gcgttcgacg ttttccatac cgtcaatcag ttgccagcct
     9601 gaaagatgga cattcatttc gcggcgggcg gcgcaggaag tgtgaacggc gactttgagc
     9661 ggttcgccct tgtcttcggg tttgaaaccg atggcaagca ggaaatgggt aaactcgatg
     9721 atgcggccgg cgcaatccac agccctttcc tcgtactcgc tgcctttaaa cagcgtcggc
     9781 cagtggtgtt tcatcatgcc gccgcacgag ccggacggca cgacgatcgg ccagttttca
     9841 gggaaaaggt cgagttgtgc tttggcgaca tcgaaggctt cggtcggatg gccggatgaa
     9901 taggcaggct ggccgcagca gctttgcgcc atcgggaaat ggacgcgtat gccctgctgc
     9961 tcgattaggg taatggcatc catgcctgct tcgggcataa aaagatcaag gacgcaagtg
    10021 ccgaaaaaat agacatcggt cggattgctg tcgtatcgga tgatttttgg gggaatggtt
    10081 gcgctcatgt tttttgataa cgggaaaccc gtttttctct gtagaaaggt aagcgtttac
    10141 tttaagtaat tgactgttgc gggtcaagtc taattttaaa aaataatccg gtttttctta
    10201 caaactgccc ccataacgct tactgtacct taatctgatg gttttcgata ataattatca
    10261 ttacaatgca atgccggttc gtttgcttgt gaacattcaa gatgccgact ctgacggcat
    10321 tcagacagca tctgaaaaca ataacggcac aaggatgggg tacaatcgcc ctatggaaaa
    10381 aaacacccta tccgcccgcg caccatcccc ttggctgccg cttctgctgg caattgccat
    10441 ttttatgcag atgttggatg cgaccatttt aaataccgca ctgcctgaaa ttgccgccga
    10501 cctgaatgag tcgcctctgg atatgcaact ggcagttatt tcctacacgc tgacggttgc
    10561 cctgctgatt cctttgagcg gttatttggc ggacaggttc ggaacgaaaa aagtcttttt
    10621 cggttcgatt gccgttttta tgctcggatc ggcattgtgc gccgcatcgg gttcgctgtt
    10681 tgaattgacg ctttcccgtg tcgttcaggg catcggcggt tcgatgctgg ttccgatacc
    10741 gcgtctgacc atcttgcgtg tgtacgacaa gtccaagctg ctcaatgcca tcaattatgc
    10801 ggttatgccc gcattaatcg ggccggtttt agggcctttg gcgggcggtt atttggtcga
    10861 atacgcttcg tggcactgga ttttcctgct caacctgccc atcggtctgc tgggtttcat
    10921 attgggacgc aacatcatgc ccgatattaa aggcagtaat atctctttag acttcaaagg
    10981 ttatctgatt ttttctgccg ccgcgtgcct cttgttactt tcggcagaaa gcctgtcgca
    11041 cgcgctgcct ccgtattttg cactgttgcc gctgtgcggc ggactgctgt ttgcacgccg
    11101 ttatttccga catatgaaaa ccgcgtccaa accgatttat tccgccgacc tgtttctgat
    11161 acgcactttc cgtctgggac tggcgggcaa tctgttcagc cgtctcggca tcagctcgat
    11221 tccttttctg atgcccctga tgtttcaaat cgctttcggc ttcggcgcaa gcctgtcggg
    11281 ttggctggtc gcacccgtcg ccctgtcttc gctgctggtc aaaccgctga ttgcaccgct
    11341 catgaaacgt ttcggctacc gcacggtact gctttggaac accaagctgc ttgccgcctt
    11401 catcatgctg ctcgccctgc ctgacggaaa ctcgccgctg tggatttggg ttttcctctc
    11461 gctggcgatc ggcgcgtgca actccctaca gttttctgcc atgaacacac tgaccctcgc
    11521 cgatttgcgc ccgcaacaaa caggcagcgg caacagcctg atggcggtca accaacagct
    11581 tgccatcagc atgggcattg ttgccggcgc attaatcctt aaaaactgga catttctgat
    11641 accggcttct tcaggtctgc attccgcctt ccgtatgacc ctgctcagca tcggcggcat
    11701 cacccttgca tcatcgctgg ttttcaaacg gctgcacgtt tcagacggca ccaacctgac
    11761 acggaacaca ccgtcctgaa gcggtccaca cgcaaaactt ttacccgttt caacgtttgg
    11821 attatgatac cgcacttcca tgcgcgccaa ccccaaaaca caggcaatgc cgtctgaaac
    11881 catatccctg atgaaaacac gcagcctaat ttccctttta tgcctccttc tctgttcatg
    11941 ttcttcatgg ttgcccccac tggaagaacg gacggaaagc cgtcatttca atacttccaa
    12001 acccgtccgc ctggacaaca tcctgcaaat ccggcacacc cctcatacca acgggctatc
    12061 cgatatctat ctgttgaacg acccccacga agcctttgcc gcccgcgccg cccttatcga
    12121 atctgccgaa cacagcctcg atttgcaata ctacatctgg cgcaacgaca tttccggcag
    12181 gctgctgttc aacctcgtgt accttgccgc agaacgcggt gtgcgcgtac gcctgctgtt
    12241 ggacgacaac aacacgcgcg gattggacga cctcctgctt gccctcgaca gccatcccaa
    12301 tatcgaagtg cgcctgttca accccttcgt cttacgaaaa tggcgcgcac tcggctacct
    12361 gaccgacttc ccccgcctca accgccgcat gcacaacaaa tcctttaccg ccgacaaccg
    12421 cgccaccata ctcggcggac gcaatatcgg cgacgaatac ttcaaagtcg gtgaggacac
    12481 cgttttcgcc gatttggaca tcctcgccac cggcagcgtc gtcggcgaag tatcgcacga
    12541 cttcgaccgc tactgggcaa gccattccgc ccacaacgcc acgcgcatca tccgcagcgg
    12601 cgacatcggc aagggtcttc aagcactcgg atacaacgac gaaacgtcca gacacgcgct
    12661 cctgcgctac cgcgaaaccg tcgaacagtc gcccctctac caaaaaatac agacaggatg
    12721 catcgactgg cagagcgtcc gaacccgcct catcagcgac gaccctgcaa aaggactcga
    12781 ccgcgaccgc cgcaaaccgc cgattgccgg gcggctgcaa gacgcgctca aacagcccga
    12841 aaaaagcgtc tatctggttt caccctattt cgttcccaca aaatccggca cagacgcact
    12901 ggcaaaactg gtgcaggacg gcatagacgt taccgttctg accaactcgc tgcaggcgac
    12961 cgacgttgcc gccgtccatt ccggctatgt caaataccga aaaccgctgc tcaaagccgg
    13021 catcaaactc tacgagctgc aacccaacca tgccgtcccc gccacaaaag acaaaggcct
    13081 gaccggcagc tccgtaacca gcctgcacgc caaaaccttc attgtggacg gcaaacgcat
    13141 cttcatcggt tcgttcaacc tcgacccccg ttccgcgcgt ctcaacaccg aaatgggcgt
    13201 tgttatcgaa agccccaaaa tcgcagaaca gatggagcgc acccttgccg ataccacacc
    13261 cgcctacgcc taccgcgtta ccctcgacag gcacaaccgc ctgcaatggc acgatcccgc
    13321 cacccgaaaa acctacccga acgaacccga agccaaactt tggaaacgca tcgccgcaaa
    13381 aatcctatcc ctgctgccca tagaaggttt attatagaaa tatagcggat taacaaaaac
    13441 cagtacgacg ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc
    13501 aagtgaatcg gtttcgtact gtttgtactg tctgcggctt cgtcgccttg tcctgatttt
    13561 tgttaatcca ctataccgtc tgaaacacct tcagacggat atccgaaccc gcaaaggaaa
    13621 aaccatgttt ccccccgaca aaaccctttt cctctgtctc agcgcactgc tcctcgcctc
    13681 atgcggcacg acctccggca aacaccgcca accgaaaccc aaacagacag tccggcaaat
    13741 ccaagccgtc cgcatcagcc acatcgaccg cacacaaggc tcgcaggaac tcatgctcca
    13801 cagcctcgga ctcatcggca cgccctacaa atggggcggc agcagcaccg caaccggctt
    13861 cgattgcagc ggcatgattc aattcgttta caaaaacgcc ctcaacgtca agctgccgcg
    13921 caccgcccgc gacatggcgg cggcaagccg caaaatcccc gacagccgcc tcaaggccgg
    13981 cgacctcgta ttcttcaaca ccggcggcgc acaccgctac tcacacgtcg gactctacat
    14041 cggcaacggc gaattcatcc atgcccccag cagcggcaaa accatcaaaa ccgaaaaact
    14101 ctccacaccg ttttacgcca aaaactacct cggcgcacat acttttttta cagaatgagc
    14161 cgcccgccgc gccgcgccgg aatcgcggca aaatgccgcc ttattccgct gtttcggtca
    14221 gcgatgagaa cacgtcgaaa taagtcggga aggttttgtg ggtgcatttc ggatcgttga
    14281 tgacgacggg tacgcccaac agcgaaacca gcgagaaaca catcgccatg cggtggtcgt
    14341 cgtacgtgtc gatgacggcg tcgggtgtca gcgtttcggg cggggtgatg tgaattgctt
    14401 cggcttcttc gacgactttt gccccgagtt tgcgcaactc gtttgccatt gcggcgatgc
    14461 ggtcggtttc tttgacgcgc cacgaaccga tgttgcgcag cgtgcaggtt tgccctgtag
    14521 caagcgcgac gatggcgagg gtcatggcgg catcggggat atggttcgca tccaaatcaa
    14581 aggattggac ggcacgttcc ttcgggcgtg aaacttcgac gaagttttcg ccccaaacca
    14641 cgtccgcccc gattttttcc agctcgcggg caaaggcgac atcgccctgt atgctgtttg
    14701 cgccgatacc ggtaacgcgg acgggcgtgg cggcaatcaa accggctgcg aggaagtagg
    14761 acgcgctgga ggcatcgcct tcgacgtgca agtgttcggg cgcgtggtag tgcgcatcgg
    14821 cgggaatttt gaagacgcgg tagccttcat tgataacctg tacgccgaat tgcgccatca
    14881 gttttaaagt aatgtcgata tagggcttgg aaatcaattc gccgaccata cggatttcaa
    14941 acgcctgccc ggtcagcggc aacgccatta aaagggcggt cagaaactgg ctggacacat
    15001 tgcctttaat cggaatcacg cgctcgccgt tgtcttggcg ttcgccgata tgaagcggcg
    15061 gatagtgttc cttgccgaga tattcgacat cggccccggc aatccgcaac gcatcgacca
    15121 aatcgccgat aggacgttcg tgcatacgag gcacgccgtg cagatgataa tcgccgccca
    15181 aaacggccag agcggcggtt aacgggcgga acgccgtgcc cgcgttgccc aaaaacaaat
    15241 cggcagtgcg gttggggaag cgtccgcctg tgccgtgcac tttcagacgg tcttcggcaa
    15301 gatattcgat ttgaacgccg agtttatcga gtgcttcgag catacggtcg gtatcgtcgg
    15361 atttgagcag ggaatggatt tcgcaagcat tgtcggacaa ggcggcaagc agcagggtgc
    15421 ggttgctgat gcttttggag ccgggcaggg cgacggtgga aggtttgaga cgggcgacgg
    15481 ggaggcggac ggattcggtc atggcaaaac gtcaatatta ttaaagataa aaacagcctg
    15541 cattatactg gtgcaaatgc tgtatgaaaa atctcaggct tggcattttc ggttttaaag
    15601 tccgtaaatg tggtttttta tgccgaaaat tgattatttt taaatttttt gtttctaaaa
    15661 tttctttgtc ggcatatttt cagcttttgt tgcggacatg gcgcgtttgc cggcggcgcg
    15721 gcacgattgc caatttcata gaatttggta gaatagccgc tgttcaacga cagacaagcc
    15781 gccgattttc cgggcggctt gtatttttat tttaatatcc ggacgcacaa aacaagacgg
    15841 tacgcggtat gcttgctgcc gcaaaccggt gtgccggaat atggagaaaa aagaccgatg
    15901 caaaaaatcc ccctgaccgt acgcggtgcg gaattgctga aacaggaatt gcagcagctc
    15961 aaaagcgtgg cgcgtcccga agtgatcgaa gcgattgccg aagcccgttc gcacggcgat
    16021 ttgtccgaaa acgccgaata cgaagccgcc aaagaacgcc aaggctttat cgagggccgc
    16081 atttccgagc tggaacacaa actttccgtt gcccacatca tcaatccgac cgaaatccac
    16141 gccgaaggca aaatcgtgtt cggtacgacg gttacgctgg aagatttgga aacggaagaa
    16201 cacgttatct atcaaattgt cggcgaagac gaagccgaca tcaaacaggg caaaatctat
    16261 gtcggctcac ccatcgcccg cgccttaatc ggcaaggaag agggggatac ggcggaagtc
    16321 caagccccgg gcggcgtgcg cgaatacgac attatcgaag tccgatatat ttgattcggc
    16381 ttgatttcga tacacccgac acacgcagga aattatagtg gattaataaa aatcaggaca
    16441 aggcgacgaa gccgaagaca gtacagatag tacgaaaccg attcacttgg tgcttcagca
    16501 ccttagagaa tcgttctctt tgagctaagg cgagacaacc ccgtactggt ttttgttcat
    16561 ccgctataac agcaaccctg tcgccgtcat tcccgcaaaa gcgggaatct ag
//
LOCUS       DAWGRZ010000028        16394 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000028 DAWGRZ010000000
VERSION     DAWGRZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16394
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.028"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            125..1189
                     /locus_tag="WHT44_000636"
     CDS             125..1189
                     /locus_tag="WHT44_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ2532857.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKEGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVAEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATINVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1330..1653
                     /locus_tag="WHT44_000637"
     CDS             1330..1653
                     /locus_tag="WHT44_000637"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ2532858.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLVEKA
                     ARTES"
     gene            1725..2048
                     /locus_tag="WHT44_000638"
     CDS             1725..2048
                     /locus_tag="WHT44_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ2532859.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQMLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2095..2298
                     /gene="tatA"
                     /locus_tag="WHT44_000639"
     CDS             2095..2298
                     /gene="tatA"
                     /locus_tag="WHT44_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ2532860.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2302..2988
                     /gene="tatB"
                     /locus_tag="WHT44_000640"
     CDS             2302..2988
                     /gene="tatB"
                     /locus_tag="WHT44_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ2532861.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMEGNLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPLPDAANTLSDGISDVMPSERSYASAETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDFRPKHR
                     AKPKLRVRKS"
     gene            3002..3772
                     /gene="tatC"
                     /locus_tag="WHT44_000641"
     CDS             3002..3772
                     /gene="tatC"
                     /locus_tag="WHT44_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ2532862.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGLMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTGQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIRPPATT"
     gene            3825..4433
                     /gene="maf"
                     /locus_tag="WHT44_000642"
     CDS             3825..4433
                     /gene="maf"
                     /locus_tag="WHT44_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ2532863.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYRVIQLPAGIDESVKAGETPFAY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRSPLSALKP"
     gene            4442..4795
                     /locus_tag="WHT44_000643"
     CDS             4442..4795
                     /locus_tag="WHT44_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ2532864.1"
                     /translation="MNRQTAYLLASFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4785..6599
                     /locus_tag="WHT44_000644"
     CDS             4785..6599
                     /locus_tag="WHT44_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ2532865.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALKLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHFGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     FQNIIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPAALGVICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIYDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6789..7466
                     /locus_tag="WHT44_000645"
     CDS             6789..7466
                     /locus_tag="WHT44_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ2532866.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7480..8886
                     /locus_tag="WHT44_000646"
     CDS             7480..8886
                     /locus_tag="WHT44_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ2532867.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9004..11430
                     /locus_tag="WHT44_000647"
     CDS             9004..11430
                     /locus_tag="WHT44_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ2532868.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASEQPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAVLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDNLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLVSFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLHAHAPKNLTLHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLAHPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALAAANPSLLAEAL
                     HLPPYRHTTHNAVQFQFDSHPLYGDILTARCNGQTTAVLPPFTTLDSRHLSRFAELDG
                     TQTLDQFLHTLTVIPEYRQHILGITLNLNGGQYSSDFLLKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     YPKTLSLRNNTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            11475..11720
                     /gene="rpsP"
                     /locus_tag="WHT44_000648"
     CDS             11475..11720
                     /gene="rpsP"
                     /locus_tag="WHT44_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ2532869.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11736..12245
                     /gene="rimM"
                     /locus_tag="WHT44_000649"
     CDS             11736..12245
                     /gene="rimM"
                     /locus_tag="WHT44_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ2532870.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNGELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            12245..12994
                     /gene="trmD"
                     /locus_tag="WHT44_000650"
     CDS             12245..12994
                     /gene="trmD"
                     /locus_tag="WHT44_000650"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ2532871.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKTQSSQAAKVIYLSPQGKPLTHQKAVEL
                     AELPHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            13010..13375
                     /gene="rplS"
                     /locus_tag="WHT44_000651"
     CDS             13010..13375
                     /gene="rplS"
                     /locus_tag="WHT44_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ2532872.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13696..14421
                     /locus_tag="WHT44_000652"
     CDS             13696..14421
                     /locus_tag="WHT44_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ2532873.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMQRFADRQIAYLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPDWCAS"
     gene            14445..15320
                     /locus_tag="WHT44_000653"
     CDS             14445..15320
                     /locus_tag="WHT44_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ2532874.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSSLTLITLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15397..16311
                     /locus_tag="WHT44_000654"
     CDS             15397..16311
                     /locus_tag="WHT44_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ2532875.1"
                     /translation="MKHLKLTLIAALLTASATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYTEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEYDPHVWNDPVLMSAYAQNVAKALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         4249 a         5254 c         3611 g         3280 t
ORIGIN      
        1 tcttgtagta aaatatctaa acagattcaa ctggaaacgg tcaagcttct tcacgccatt
       61 cccatttcaa ccgccgcctg ccagattcag acggtattcc tacttgaaaa caaggagcgt
      121 tacaatgaaa gcagcacgtt tttacgacaa aggcgacatc cgcatcgaag acatccccga
      181 accgaccgtc gcccccggca ctgtcggcat caatgtcgcc tggtgcggca tctgcggtac
      241 tgacctgcac gaattcatgg aaggcccgat tttcattccg ccttgcggtc atccgcaccc
      301 gatttccggc gagtccgcac ccgtaacgat gggacacgag ttctccggcg tggtctatgc
      361 cgtcggcgaa ggcgtggacg acatcaaagt cggccaacac gtcgtggtcg aaccctacat
      421 catccgcgat gacgtaccga ccggagaagg cagcaactac cacctctcca aagatatgaa
      481 ctttatcggc ttgggcggct gcggcggcgg tctgtccgaa aaaatcgccg tcaaacgccg
      541 ttgggtgcat ccgatttccg acaaaatccc gttggatcaa gccgctttga tcgaaccgct
      601 gtctgtcgga caccacgcct atgtacgcag cggcgcgaaa gaaggcgacg tcgcattggt
      661 cggcggtgca ggtccgatcg gtttgctgtt ggctgccgtg ttgaaagcca aaggcatcaa
      721 agtcatcatc accgagttga gtaaagcacg caaagacaaa gcgcgcgaat ccggcgttgc
      781 cgactacatc ctcgacccgt ccgaagtcga tgttgttgca gaagtgaaaa aactgaccaa
      841 cggcgaaggc gtggacgtgg catttgagtg caccagcgtc aacaaagtgt tggatacttt
      901 ggtcgaagcc tgcaaacctg ccgccaattt ggttatcgta tccatctgga gccaccccgc
      961 caccatcaac gtccacagcg tcgtgatgaa agagttggac gtgcgcggca cgattgccta
     1021 ctgcaacgac cacgccgaaa ccatcaaact ggtcgaagaa ggcaaaatca accttgagcc
     1081 tttcatcacc cagcgcatca agctggacga gctggtttcc gaaggcttcg agcgtctgat
     1141 tcacaacaac gaatccgccg ttaaaattat tgtgagtcca aacctgtaag caaattacat
     1201 ttgcaacagc acaaatgccg tctgaaacgc tcagacggca ttttccaacg aagcgcaaca
     1261 ggcgtattgc ccgagagcag ccgtttagcc tatcattccg aacaaacaga aacaaacaga
     1321 aggaaaatca tgggagattc cgtactatcc gccatccaac aaaccatcac ccagcgaaaa
     1381 tctgccaatc cgtccgaatc ttacgtcgca cagctcttgc ataagggcga agacaaaatc
     1441 ctaaaaaaag tgattgaaga ggcgggcgaa gtgttgatgg catccaaaga caaaaacccg
     1501 tcccacctgg tttacgaagt tgccgactta tggtttcaca ccatgattct tctgacacac
     1561 cacgacctga aggcggaaga cgtattggac gaacttgcgc gccgtcaggg gctgtcgggg
     1621 ctggtcgaaa aagccgctcg cacagaatct tgaatttata ttaaaatccg cactttccca
     1681 cattcaatcc gtctgaccgc ttttcagacg gcatcggagc cgttatggac aactgtattt
     1741 tctgcaaaat cgccgccaaa gacattccgg cgcaaaccgt ctatgaagac ggcgaaatgg
     1801 tttgtttcaa agacatcaac cccgctgctc cggttcatct gctgctgatt cccaaagtcc
     1861 atttcgattc gttggcacac gccgcgcccg aacatcagat gctgctgggc aaaatgatgt
     1921 tgaaagttcc cgaaatcgcc aaagcggcag gactggcaga cggcttcaaa accctgatca
     1981 ataccggaaa aggcggcgga caagaggtct tccacctgca tatacacatc atgggtacac
     2041 ccgtataaac cgttatttca caatcaaccc ctaatactta cttaaggata catcatgggc
     2101 agtttttctc tgacgcactg gattatcgta ctgattatcg tcgttttgat attcggcacc
     2161 aaaaaactgc gcaacgtcgg caaagacctc ggcggtgcgg ttcatgactt caaacagggg
     2221 ctgaacgaag gtacagacgg caaagaagcc caaaaagacg atgtaatcga acacaaaaaa
     2281 gacgaagaca aagcgtaatt tatgtttgat ttcggtttgg gcgagctggt ttttgtcggc
     2341 attatcgccc tgattgtcct cggccccgaa cgcctgcccg aggccgcccg caccgccgga
     2401 cggctcatcg gcaggctgca acgctttgtc ggcagcgtca aacaggaatt tgacactcaa
     2461 atcgaactgg aagaactgag gaaggcaaag caggaatttg aagctgccgc cgctcaggtt
     2521 cgagacagcc tcaaagaaac cggtacggat atggaaggca atctgcacga catttccgac
     2581 ggtctgaagc cttgggaaaa actgcccgaa cagcggacac ctgccgattt cggtgtcgat
     2641 gaaaacggca atccgcttcc cgatgcggca aacaccctat cagacggcat ttccgacgtt
     2701 atgccgtccg aacgttccta cgcttccgcc gaaacccttg gggacagcgg gcaaaccggc
     2761 agtacagccg aacccgcgga aaccgaccaa gaccgcgcat ggcgggaata cctgactgct
     2821 tctgccgccg cacccgtcgt acagaccgtc gaagtcagct atatcgatac tgctgttgaa
     2881 acgcctgttc cgcacaccac ttccctgcgc aaacaggcaa taagccgcaa acgcgatttt
     2941 cgtccgaaac accgcgccaa acctaaattg cgcgtccgta aatcataaag agagcaatcc
     3001 ggtgtccgaa acacaaaacg aacaacccgt ccaaccgctt gtcgagcatc tcatcgagct
     3061 gcgccgccgc ctgatgtgga cggttgtcgg catcttagtc tgctttttcg gcctaatgcc
     3121 gtttgcccaa caactctata cttttatcgc cgacccgctg atggcaaacc tgcccaaaga
     3181 caccagcatg attgccaccg atgtcatcgc accatttttc gtgccggtca aagttaccct
     3241 gatggcggca tttttaattt cgctgccgca tacgctctac caaatctggg catttgtcgc
     3301 gcccgcactc taccaaaacg aaaaacgcct gattacgccg ctcgtcctct ccagcgtcag
     3361 cctgtttttc atcggcatgg catttgccta ctttttggtt ttccccgtca ttttcaaatt
     3421 ccttgccagc gttacccctg tcggtgtcaa tatggcgaca gacatcgaca aatacctctc
     3481 cttcatcttg gggatgtttg ttgcgttcgg cacaacgttt gaagtcccca ttgtcgttat
     3541 cctgttaacc aaaattggtg tggtaacaac cggacagctc aaacgcgccc gcccctatgt
     3601 gattgtcggc gcgtttgtca ttgccgccat catcacgccg cccgatgtga tttcacaaac
     3661 cctgcttgcc attccgctga ttctcttata cgaagcaggt atttggttcg gacgcttttt
     3721 cacgccacgt tcagaacagg atggcgacat acggccgcct gcaacaacct gacactatgc
     3781 cgtccgaacc tccgcctcat accgcaacag attaaggaat acctttgaat accctctatt
     3841 taggttcaaa cagcccgcgc cgaatggaaa tcctgacaca gttgggctac cgcgtcatcc
     3901 aactgcctgc cggcatcgac gaatccgtta aagccggcga aacacctttc gcttacgttc
     3961 aaaggatggc agaagaaaaa aaccgaaccg ccctgaccct cttttgcgaa accaacggca
     4021 caatgcccga tttccccctg attaccgccg acacctgcgt cgtttcagac ggcatcatat
     4081 tgggcaaacc ccgctcccaa gccgaagcaa tcgaattttt aaaccgattg tccggcaaac
     4141 aacataccgt cctgactgct gtctgcattc attatcgcgg caaaacgtca agccgcgtcc
     4201 aaaccaaccg cgtcgttttc aagcccctga gttcggaaga aatttccgcc tatgtgcaaa
     4261 gcggcgagcc gatggacaaa gccggtgcct acgccgtaca aggcataggc ggcatcttta
     4321 tccaatctat cgaaggcagc ttcagcggca ttatgggact gcccgtttat gaaaccgttt
     4381 cgatgttgca ggatttggga taccgctccc ccttgtccgc ccttaaaccg taaagaccgc
     4441 cgtgaacaga caaaccgctt acctccttgc ctctttcagc ctgatcgcac tgataatcct
     4501 gtccctttcc tgggaactgt ggattgcacc gttgcgcccg ggcggctcgt ggctcgcgct
     4561 caaagccctc cccctctgtc tgccgctttc aggcatcttg aaaaagaaaa tctatactta
     4621 ccaatacagc tccatgctgg ttctgattta ctttgccgaa gccgtcatgc gcctgttcga
     4681 cgcctatccc gcagaacaga tttgcgccgc gctttccgca gtattcagca tcatcttctt
     4741 catatcctgc ctgtcgttcg tcaaacaata caaggaaaca aacaatgccc gctgaaacga
     4801 ccgtatccgg cgcgcacccc gccgccaaac tgccgattta catcctgccc tgcttccttt
     4861 ggataggcat cgtccccttt accttcgcgc tcaaactgaa accgtcgccc gacttttacc
     4921 acgatgccgc cgccgcagcc ggcctgattg tcctgttgtt cctcacggca ggaaaaaaac
     4981 tgtttgatgt caaaatcccc gccatcagct tccttctgtt tgcaatggcg gcgttttggt
     5041 atcttcaggc acgcctgatg aacctgattt accccggtat gaacgacatc gtctcttgga
     5101 ttttcatctt gctcgccgtc agcgcgtggg cctgccggag cttggtcgca cacttcggac
     5161 aagaacgcat cgtgaccctg tttgcctggt cgctgcttat cggctccctg cttcaatcct
     5221 gcatcgtcgt catccagttt gccggctggg aagacacccc tctgtttcaa aacatcatcg
     5281 tttacagcgg gcaaggcgta atcggacaca tcgggcagcg caacaacctc ggacactacc
     5341 tcatgtgggg catactcgcc gccgcctacc tcaacggaca acgaaaaatc cccgccgccc
     5401 tcggcgtaat ctgcctgatt atgcagaccg ccgttttagg tttggtcaac tcgcgcacca
     5461 tcttgaccta catagccgcc atcgccctca tccttccctt ctggtatttc cgttcggaca
     5521 aatccaacag gcggacgatg ctcggcatag ccgcagccgt attccttacc gcgctgttcc
     5581 aattttccat gaacaccatt ctggaaacct ttactggcat ccgctacgaa actgccgtcg
     5641 aacgcgtcgc caacggcggt ttcacagact tgccgcgcca aatcgaatgg aataaagccc
     5701 ttgccgcctt ccagtccgcc ccgatattcg ggcacggctg gaacagtttt gcccaacaaa
     5761 ccttcctcat caatgccgaa cagcacaaca tatacgacaa cctcctcagc aacttgttca
     5821 cccattccca caacatcgtc ctccaactcc ttgcagagat gggaatcagc ggcacgcttc
     5881 tggttgccgc aaccctgctg acgggcattg ccgggctgct taaacgcccc ctgacccccg
     5941 catcgctttt cctaatctgc acgcttgccg tcagtatgtg ccacagtatg ctcgaatatc
     6001 ctttgtggta tgtctatttc ctcatccctt tcggactgat gctcttcctg tcccccgcag
     6061 aggcttcaga cggcatcgcc ttcaaaaaag ccgccaatct cggcatactg accgcctccg
     6121 ccgccatatt cgcaggattg ctgcacttgg actggacata cacccggctg gttaacgcct
     6181 tttcccccgc cactgacgac agtgccaaaa ccctcaaccg gaaaatcaac gagttgcgct
     6241 atatttccgc aaacagtccg atgctgtcct tttatgccga cttctccctc gtaaacttcg
     6301 ccctgccgga ataccccgaa acccagactt gggcggaaga agcaaccctc aaatcactaa
     6361 aataccgccc ccactccgcc acctaccgca tcgccctcta cctgatgcgg caaggcaaag
     6421 ttgcagaagc aaaacaatgg atgcgggcga cacagtccta ttacccctac ctgatgcccc
     6481 gatacgccga cgaaatccgc aaactgcccg tatgggcgcc gctgctaccc gaactgctca
     6541 aagactgcaa agccttcgcc gccgcgcccg gtcatccgga agcaaaaccc tgcaaatgac
     6601 ccgcgccggc ggatgcggat accgcccgaa atgtaaagct ccatgcaaga cattgcaaaa
     6661 aacagcaaac cggtagggaa atacgctatc aaaaaacatt gcagccgtgt taagataaac
     6721 cgtcaaacaa tcttttcacg ccccgcccga aacagggtcg gggcataccc ttacgaaaag
     6781 gaaacaccat gagccgcgta ttactcgtag atgacgatgc cctgctaacc gaactgctga
     6841 ccgaatacct gagcgccgaa ggtctgaacg tccgcagcgt tcccgacggg gaagcaggcg
     6901 tacaggaaat cctgagcggg cagtacgatg tagtcgtatt ggattccatg atgcccaaaa
     6961 tgaacggctt ggatgtcttg aaaaacgtac gcgcccgaag caccgtcccc atcatcatgc
     7021 tgaccgccaa aggcgacgac atcgaccgaa tcatcggctt ggaaatgggc gcggacgact
     7081 atgtccccaa accctgcaca ccacgcgaac tcttggcacg catcaatgcc atcctgcgcc
     7141 gcgcacaaca cagcggcgaa cagaacaacg cacccaacag catctccgtc agcgatgtcg
     7201 tcctgtaccc cgccaaacgc caggcatccg tcaaagacat gccgctcgaa ctgaccagca
     7261 ccgaattcaa cctgctcgaa gtcctgatgc gccatgccgg acaggtagtc agcaaagaaa
     7321 ccctgtccgt cgaagcactc gaccgcaagc tggcaaaatt cgaccgcagt atcgacgtac
     7381 acatctccag catccgccac aagttgggcg atgcctctct gattcaaacc gtacgcggct
     7441 tgggctacct gtttgtcaaa aactgaaata aacagataaa tgaaactgtt ccaacgcatt
     7501 ttcgccacat tttgcgcggt tatcgtctgt gcaatctttg tggcgagttt ttctttctgg
     7561 ctggtgcaga acacccttgc cgaaaaccag ttcaaccaac gccgcaccat cgaaaccact
     7621 ttgatgggca gcatcatttc cgcattccgg gcacgcgggg acgcgggtgc gcgcgaaatc
     7681 ctgacggaat ggaaagacag ccccgtctca tcgggcgtgt acgttataca gggcgacgag
     7741 aaaaaagata tcctgaaccg gtatatcgac agctatacca tcgaacgcgc ccggcttttc
     7801 gccgccggac acccgcattc caacctcgtc catatcgaat acgaccgctt cggcgaagaa
     7861 tacctgttct tcaccaaaga ctgggacaaa ctccaagccc gccgcctgcc cagccccctg
     7921 ttgatccccg gcctgccgct cgccccgatt tggcacgaac tcatcatatt gtccttcatc
     7981 atcatcgtcg gactgctgat ggcatatatc ctcgccggca acattgccaa acccatcaga
     8041 atcttaggca acggcatgga cagggtggca aacggagaac ttgaaacccg tatctcccaa
     8101 caggtcgacg accgcgacga cgaattgtcc catcttgcca tccaattcga caaaatggtg
     8161 gaaaaactcg aaaaactcgt tgccaaagaa cgccacctgc tccatcacgt ctcccatgaa
     8221 atgcgttctc cccttgcgcg catgcaggca attgtcggac tgattcaggc gcagccccaa
     8281 aaacaggagc aatatctcaa acggctggaa ggcgaactga cccgcatgga tacgctggcc
     8341 ggggaactgt taaccctgtc ccgtctcgaa acttccaata tggctttgga aaaagaaagc
     8401 ctgaaactcc tgcccttcct gggcaacctg gtagaagaca atcaaagcat tgcccagaaa
     8461 aacggacaaa cggttaccct gtctgccgac ggaaaaatcc ccgaaaacac aaccatcctt
     8521 gccaacgaaa gctacctgta ccgcgccttc gacaacgtca tccgcaacgc cgtcaactac
     8581 agtcccgaag gcagcaccat cctgatcaac atcggacaag accacaaaca ctggataatc
     8641 gacgttaccg acaacggccc cggcgtggac gaaatgcagc tcccgcacat cttcaccgct
     8701 ttctaccgtg cagactccag tgccaacaaa cccggaacag gactggggct tgcattgacc
     8761 caacatatta ttgaacagca ctgcggcaaa atcatcgccg aaaacatcaa accgaacggt
     8821 ctgcggatgc gctttatcct gcccaagaaa aaaaccggtt ccaaaacaga aaaaagtgcg
     8881 aactgaccat aataccgtct gaaccggctt cagacggcat tgcacaaaca gttaccccaa
     8941 aacaaacaca cagcgggtgt ctctccaaaa atcacgcaca taccgtccgc aaaggaatat
     9001 atcatgtcgg cacaaaccga tccgggctac ttcttcatgc ccaaccacat catcctgata
     9061 ggcgcgagcg aacaaccgta cagcctgggc gaacgtgtac tcagcaacct gctgagtacg
     9121 ccctttcaag gaaaaatcac ccccgtaaac ccgcgccacc acaccatagc cgtactgccc
     9181 gcctacacca gcctcaacaa aatccccggc aatgcagacc tgattattgc cgttacccta
     9241 cccgacagtt acgacaccct cttcaaaacc tgccgcaaaa agcagctccg acacatcatc
     9301 ctcatacagg actgggacaa cctgtctgcc gcagaactgc acaccgccga aactgccatc
     9361 cgcaaacacc acggcaacgg actcaacatc accgcctgca ccaccgcagg catccaactg
     9421 ccctcactcg gactcaacat cagtacccaa gacggatacg ccgcaggcca taccgccata
     9481 ctgaccggca atgccgccgt cagccgccaa atcgacaaca tcctgaacaa actccgtcaa
     9541 ggcacatccc gccacatcag cctgcatccc ggcatcagcc ccatcacatc cgccgattgg
     9601 ctcaaccgct tcggacacag cctgcacacc aaaaccgccg tcctacacca caaccctgaa
     9661 gaggatcagc gcaaactgtt cagcgcaatc cgccaattta cccgccatac gccgctgatt
     9721 ctccacatca cctgccgcac gacagaaacc gaccgtgccg tactgcactg cctcgcccga
     9781 cactgcaact tcctcgtcag tttcaacgcc gacgacctcg aagccgcact gcgcgcccaa
     9841 ctgtccgacc ttccacccct gtcccgactc gacatcctgt ccgacacgcc tgccgaatgg
     9901 ctgcacgcgc acgcgccaaa aaacctcacc ctccactttc ccaaccttcc ccaccacatc
     9961 cgcaacggac acctgaccgg cacacccaca ccctcaatct gccacgacat cgcctcacgt
    10021 cagcttgccc accccgacac ccaagccgtc ctaaccatcc tcagtccctc cggacacgag
    10081 gattacaaaa aaacagcacg cgcccttatc cgcctgtccg aacagaccgc caaacccctg
    10141 ctcgtcagca gccccttttc agacggcata acccatttcg acacccccac tcaggcaatc
    10201 cgcacccttt cctaccgcaa caccgccgcc gccctgaaac aggcacagct cgacattgca
    10261 ccgccgcagc catgccgtct gaaaacaccg caaccccaaa acatcaaaaa agcccttgca
    10321 gcggcaaacc cctccctgct cgccgaagcc ctgcacctcc ccccctaccg gcacaccacc
    10381 cataacgccg tacaattcca attcgacagc caccccctct atggcgacat cctgaccgca
    10441 cgctgcaacg gacaaaccac tgccgtactc ccgccgttta ccacgctcga cagccgccac
    10501 ctttcccgct ttgccgaact cgacggcaca caaaccctcg accagttcct gcacacactg
    10561 accgtcattc ccgaataccg ccaacacatt ctcggcatca ccctcaacct caacggcgga
    10621 caatacagca gcgatttcct cttaaagaca cccgaaacac acgacacgcc caaacgcaag
    10681 aacacaggca aagccgccca aaccctcgaa catgccgccg caaaaatgca gagtgccgcc
    10741 gcatacctga aacacaaaaa cccgacagcc gccgaatttc tccgccacac aagcgaagcc
    10801 gccgcagaac tgctcggcag caaaaccgaa accggagcag ccgtacccaa cgtacttgcc
    10861 ccctatcccg cagcataccc caaaacactg tccctaagaa acaacacgac cgttaccatt
    10921 acccccattt tgcccgaaga cgcagaagcc aaacagcagt tcgtccgcag cctcggtccc
    10981 gaagcacggt acacacgctt catgacccac accaacgaac tgcccgcagc cacgttggca
    11041 cgcctgtgca accccgatta ccactgtgaa gccgcatgga cggcaaagga tgccgacagc
    11101 aacatcgtcg ccgtcgtccg ccacagccgc ctgaatcgca acgaatgcga attcggcatc
    11161 acactggcgg aacatatgcg cggcagcgga ctggcacaga aaatgatgga actcatcatc
    11221 caaaccgccg cacagcaagg ctaccggact atgagtgccg acattctcaa aaccaatacc
    11281 cccatgatca aacttgctga aaaatcagga tttaccctca aggaatcgga caccgaaaaa
    11341 aacctgtacc gcgcatatct gaaccttgcg gcagacaaaa caacagaaaa aacaaataaa
    11401 aacttgcgca ccgaccacaa aataacctaa aatcggcagt ttccatatat ccgattttct
    11461 caaaaggact caaaatggta gttatccgtt tggcacgcgg cggctcgaaa caccgcccct
    11521 tctacaacgt catcgttact gactcacgca gccgccgcga cggccgcttc atcgaacgcg
    11581 taggcttcta caaccccgta gccaacgaaa aacaagagcg cgtccgcctc aatgcagacc
    11641 gcctgaacca ctggattgca caaggcgcgc aagtcagcga ctccgttgca aaactgatta
    11701 aagaacaaaa agccgcctaa tccgcatttg ccgccatgac agacactcaa aaccgggtag
    11761 ccatgggcta catcaaaggc gtattcggca taaaaggctg gttgaaaatt gccgccaaca
    11821 ccgaatattc cgacagcctt ttggactacc ccgagtggca tttggtcaag gacggcaaaa
    11881 ccatcagcgt tacccttgaa gccggaaaag tcgtcaacgg cgaactccaa gtcaaattcg
    11941 aaggcataaa cgaccgcgac ttggcattct cattgcgcgg ttacaccatc gaaatacccc
    12001 gtgaagcatt cgccccgaca gaagaagacg aatactactg gacagacttg gtcggcatga
    12061 ccgttgtcaa caaagaccat accgttttag gcaaggtaag caacctgatg gaaaccggcg
    12121 caaacgacgt attgatgatt gacggagaac acgggcagat tctgattccg ttcgtttccc
    12181 aatatatcga aaccgtcgat accggcagca agaccattac tgccgactgg ggtttggact
    12241 actgatgctt atccaggcag ttaccatttt ccccgaaatg ttcgacagca ttacccgcta
    12301 cggcgtaacg ggacgcgcga acagacaggg aatctggcag tttgaagcag tcaatccccg
    12361 aaagtttgcc gacaacagat tgggctacat cgacgaccgc ccgttcggcg gcggcccggg
    12421 aatgattatg atggctccgc cgcttcatgc ggcaatagaa cacgccaaaa cacaatcctc
    12481 ccaagctgca aaagtcatct acctcagccc ccaagggaaa ccgctgacac accaaaaagc
    12541 ggtagaactg gcagaacttc cgcatctgat tctgctgtgc ggacggtatg agggcataga
    12601 cgaaaggctt ctgcaaagca gcgtcgatga agaaatcagc atcggagact tcgttgtttc
    12661 cggcggagag cttcccgcca tgatgctgat ggatgcggta ttgaggctcg tacccggcgt
    12721 attgggcgat atgcagtctg ccgaacagga ttcgttctca agcggcattt tggactgccc
    12781 ccactacacc aaacccttag aatttcaagg tatggctgtt ccggaagtat tgcgttccgg
    12841 caatcatggc ttgatagcgg aatggcggtt ggaacaatcg ctgcgccgca ccttggagcg
    12901 cagacccgat cttttggaaa agcgcgtttt aatcccaaag gaatcccgcc tcttagaaac
    12961 catccggcaa gagcaacggg aaatccaatc ataatttagg aaaaaaacaa tgaacctgat
    13021 tcaacagctc gagcaagaag aaattgcccg cctgaataaa gaaatccccg aattcgcacc
    13081 gggcgacacc gtagtcgtat ccgtacgcgt cgtggaaggt acccgcagcc gtctgcaagc
    13141 ctacgaaggc gtggttattg cccgtcgcaa ccgtggtctg aacagcaact tcatcgtccg
    13201 caaaatctcc agcggcgaag gtgttgaacg tactttccaa ctgtactctc cgaccgtcga
    13261 aaaaatcgaa gtcaaacgcc gtggcgacgt acgccgtgcc aaactgtact acctgcgcgg
    13321 cctgaccggt aaagctgcac gcatcaaaga aaaactgcct gcacgcaaag gttgattcaa
    13381 accgttttcc cccaatgccg tctgaacctt cagacggcat ttcttattgc tgcgatgccg
    13441 gcaagtcaac gatatgcctg cccctcatcc accttgatta acaatcactc ccgcctattc
    13501 aagcataatt tacagaccaa acgttataca gtatcatatc agccatataa cgataaacgc
    13561 tttaccgcct tccctttcaa aaccgcaaat catcatgaca ccttcccttt tactatcagg
    13621 attgaccttc cgcctcatcc ttgccctgat tgccgtatcc cttttatggg gcgtttactt
    13681 ttgggcagta tccgcatgag catcattgtc gaaaacctga ccgtcagcta ccgccgccga
    13741 cctgccgtgc accatgtgga cattactttt gaagaacata gtatgtgggc ggttttcggt
    13801 cccaacggcg cagggaaatc cacctttctc aaatccttga tgggattgca gcctatcgat
    13861 acaggcagca tccggctgga cggattgacc cgtcagaaca tcgcctacct tccccagcag
    13921 tccgatatcg accgctccca gcctatgacc gttttcgact tggcggcaat ggggctatgg
    13981 tatgaaatcg gctttttcaa agggataaat accgctcaaa aacaacgcgt tcacgaagcc
    14041 ttggagcgcg tcggaatgca acggtttgcc gaccgtcaga ttgcctatct ctcaaacgga
    14101 caatttcagc gtgtcctttt tgcccgaatg ctggttcaaa atgccaaatt cctgctgctc
    14161 gacgaaccct tcaatgccgt tgatgcacgg acaacctacg agcttctcga cgtattgcag
    14221 aaatgccatt gcggcggaca cgccatcatc gccgtactgc acgattacga acaagtccgt
    14281 gcctactttc ccaataccct gctgctcgcc cgcgaaaaaa ttgcggcagg cgcaaccgag
    14341 accattctga cagaacccta cctcgcccaa gccaacgcca aaatgcagca acaggaaagc
    14401 cccgactggt gcgcctcata aatgccgtct gaaaccgaaa aaccatgaat ctctacgacc
    14461 tgctccttgc cccctttgca gaattcgact ttatgcgcta cgccctcgca tccgtcttct
    14521 gcctgtccct cagtgccgca cccgtcggcg tattcctcgt catgcgccgt atgagcctga
    14581 taggcgacgc attgagccac gccgtcctgc ccggtgccgc cgtcggctac atgtttgccg
    14641 gcttgagcct gcccgccatg ggtttgggcg gcgtagccgc aggcatgctg atggcactgc
    14701 ttgccggact cgtcagccgc ttcaccaccc tgaaagaaga tgccaacttt gccgcctttt
    14761 atctcagcag cctcgccatc ggcgtagtcc tcgtcagcaa aaacgggagc agcgtcgatt
    14821 tgctccacct ccttttcggc tctgtacttg ccgtcgatat tcctgccctg cagctcatcg
    14881 ccgccgtctc cagcctcacg ctcattaccc ttgccgtcat ctaccgcccg ctcgtactcg
    14941 aaagcatcga ccccctgttt ctcaaatccg tcggcggcaa aggcgggctt tggcacgtcc
    15001 tctttctcgt cctggtcgtc atgaacctcg tatccggctt tcaagccctc ggcacactca
    15061 tgtccgtcgg actcatgatg ctgccagcca ttaccgcccg cctgtgggcg aagcatatgg
    15121 gcgcactcat cctcctatcc gttctgacag ccctgctgtg cggcttgagc ggactgctca
    15181 tttcctacca catcgaaatt ccttccggtc ccgccatcat cctctgttgc agcgtccttt
    15241 atctcttttc cgtcatactc ggcaaagaag gcggcattct gaccaaatgg ctcaaaaacc
    15301 accgccacca caccacctga ccccgcaagc cgcttgaaca aacaggcggc ttgttttacc
    15361 ataacagatg ttacgttata tcacaggaga acagttatga aacacctcaa actcaccctt
    15421 attgccgcat tgctgaccgc ctccgcaact gccgcccccc tgccggttgt aaccagcttc
    15481 agcattttag gcgacgtagc caaacaaatc ggcggagagc gcgtatccat acaaagtttg
    15541 gtcggagcca accaagatac gcacgcctat catatgacca gtggcgacat taaaaaaatc
    15601 cgcagtgcaa aactcgtcct gctcaacggc ttaggacttg aagctgccga tgtgcaacgc
    15661 gccgtcaaac aaagcaaagt atcctatacc gaagcgacca aaggcatcca acccctcaaa
    15721 gccgaagaag aaggcggaca ccatcacgac cacgatcatg accacgaagg acaccaccat
    15781 gaccacggcg aatatgaccc gcacgtctgg aacgaccccg tccttatgtc cgcctatgcc
    15841 caaaacgttg ccaaagccct gataaaggcc gatcccgaag gcaaagttta ttatcaacaa
    15901 cgcttgggca actaccaaat gcagctcaaa aaactgcaca gcgacgcaca agccgcattt
    15961 aatgccgtcc ctgctgccaa acgcaaagtc ctgaccgggc acgatgcctt ttcctatatg
    16021 ggcaaacgtt accatatcga attcatcgcc ccgcaaggcg tgagcagcga agccgagcct
    16081 tcggccaaac aagtcgccgc catcatccga caaatcaaac gcgaaggcat caaagccgtc
    16141 tttaccgaaa acatcaagga cacccgtatg gttgaccgta tcgccaaaga aaccggtgtc
    16201 aacgtcagcg gcaaactgta ttccgacgca ctcggcaacg cgcccgcaga cacctacatc
    16261 ggaatgtacc gccacaacat caaagccttg accaacgcga tgaagcaata aaaccgtttg
    16321 attggtttga atttgaaaag gcaaaaggcc gtctgaaaat tatagtggat taacaaaaat
    16381 caggacaagg cgac
//
LOCUS       DAWGRZ010000029        16367 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000029 DAWGRZ010000000
VERSION     DAWGRZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16367
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.029"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            6..81
                     /locus_tag="WHT44_000655"
     tRNA            6..81
                     /locus_tag="WHT44_000655"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Trp,seq:cca)
     gene            196..474
                     /gene="secE"
                     /locus_tag="WHT44_000656"
     CDS             196..474
                     /gene="secE"
                     /locus_tag="WHT44_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ2532876.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            476..1012
                     /gene="nusG"
                     /locus_tag="WHT44_000657"
     CDS             476..1012
                     /gene="nusG"
                     /locus_tag="WHT44_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ2532877.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1113..1547
                     /gene="rplK"
                     /locus_tag="WHT44_000658"
     CDS             1113..1547
                     /gene="rplK"
                     /locus_tag="WHT44_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ2532878.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1547..2242
                     /gene="rplA"
                     /locus_tag="WHT44_000659"
     CDS             1547..2242
                     /gene="rplA"
                     /locus_tag="WHT44_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ2532879.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            2472..2972
                     /gene="rplJ"
                     /locus_tag="WHT44_000660"
     CDS             2472..2972
                     /gene="rplJ"
                     /locus_tag="WHT44_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ2532880.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3030..3401
                     /gene="rplL"
                     /locus_tag="WHT44_000661"
     CDS             3030..3401
                     /gene="rplL"
                     /locus_tag="WHT44_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ2532881.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            3591..7769
                     /gene="rpoB"
                     /locus_tag="WHT44_000662"
     CDS             3591..7769
                     /gene="rpoB"
                     /locus_tag="WHT44_000662"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ2532882.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVADRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDFGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            7926..12101
                     /gene="rpoC"
                     /locus_tag="WHT44_000663"
     CDS             7926..12101
                     /gene="rpoC"
                     /locus_tag="WHT44_000663"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ2532883.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRAGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQE
                     VEQETAETQVTDE"
     gene            12393..12650
                     /locus_tag="WHT44_000664"
     CDS             12393..12650
                     /locus_tag="WHT44_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532884.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRMRMPINIG"
     gene            12647..12796
                     /locus_tag="WHT44_000665"
     CDS             12647..12796
                     /locus_tag="WHT44_000665"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ2532885.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            12975..13346
                     /gene="rpsL"
                     /locus_tag="WHT44_000666"
     CDS             12975..13346
                     /gene="rpsL"
                     /locus_tag="WHT44_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ2532886.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            13464..13934
                     /gene="rpsG"
                     /locus_tag="WHT44_000667"
     CDS             13464..13934
                     /gene="rpsG"
                     /locus_tag="WHT44_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ2532887.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            13953..16058
                     /gene="fusA"
                     /locus_tag="WHT44_000668"
     CDS             13953..16058
                     /gene="fusA"
                     /locus_tag="WHT44_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ2532888.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEVDKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            16144..>16367
                     /locus_tag="WHT44_000669"
     CDS             16144..>16367
                     /locus_tag="WHT44_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ2532889.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILSKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETR"
BASE COUNT         4380 a         3636 c         4212 g         4139 t
ORIGIN      
        1 tttagaggcc aatagctcaa ttggtagagt atcggtctcc aaaaccgagg gttgggggtt
       61 cgagaccctc ttggcctgcc aaataaaaaa ttaaccggcc ttgtgtcggt taattttttt
      121 gtatttgtta tttagtaaac tctcttgcca tttacatgga ttgagaatag acagatgcta
      181 tgatggataa ataatatgac agaacatacg cctgaaaaaa agaacgttaa agtggatcaa
      241 ctggttgttc aagataaaga atctgcatct aattccggta aggaagggtt ttttgcatat
      301 ttctcaaatt cttggtccga attcaaaaag gtggtttggc ctaagcgtga agatgctgtc
      361 agaatgactg tatttgttat agtgtttgtt gctgtgcttt ctatatttat ctatgcggca
      421 gatacagcaa tttcgtggtt attttttgat gtattgctga gaagggaagg ttgagatgtc
      481 gaaaaaatgg tatgttgtac aggcgtattc ggggtttgag aagaatgtcc aacgaatatt
      541 ggaagagcgc attgcccgtg aggagatggg agattatttc ggacaaattc tggtgcctgt
      601 agagaaagtt gttgatatcc gcaatggtcg taagactatt agtgaaagaa agtcatatcc
      661 tggttatgtg ctagttgaga tggaaatgac agatgactct tggcatcttg taaaaagcac
      721 cccccgtgtt tccggtttta ttggagggag ggctaataga cctacgccga ttagtcagag
      781 agaggctgaa attattttac agcaggttca gaccggcata gagaagccga aaccaaaagt
      841 tgaatttgag gtcggtcaac aggttcgtgt aaatgaaggg ccgtttgcgg attttaacgg
      901 ggtggttgag gaggtcaatt atgaacggaa taagttacgc gtgtctgttc agatatttgg
      961 tagagaaaca cccgttgagc tggagttcag ccaggttgaa aagattaact gatttttata
     1021 cttgaaaaaa aagcaataag aggatagaat caaaaattaa cttggggagc ggaaatggtt
     1081 ccgcgtctta cccgttttta ggagttcgtt aagtggcaaa gaaaattatc ggctatatta
     1141 aactgcaaat tcctgcaggt aaagccaatc catctcctcc ggttggtcct gctttgggtc
     1201 agcgcggttt gaatattatg gaattttgta aggcatttaa tgctgcaacc caaggtatgg
     1261 agcctggctt accgattccg gttgtgatta ctgcatttgc agataaatca ttcacatttg
     1321 tgatgaaaac cccgccagct tctatcttgt tgaaaaaggc tgccggtttg caaaaaggta
     1381 gttctaatcc tctgaccaac aaagtgggta aattgacccg tgcccagttg gaagaaattg
     1441 ctaaaactaa agatcctgat ttgactgctg ctgacttgga tgcggctgtc cgtactatag
     1501 caggttctgc tcgctcaatg ggcttggatg tggagggtgt tgtataatgg ctaaagtatc
     1561 taaacgcttg aaagctcttc gctcttctgt ggaagccaat aaattatatg caattgatga
     1621 agcaattgct ttggtaaaaa aagcagcgac tgctaaattt gacgagtctg ttgacgtatc
     1681 tttcaacttg ggcgttgatc cgcgtaaatc tgaccaagtt atccgtggtt cggtcgttct
     1741 gcctaaaggc accggtaaga taacccgtgt ggctgtattt actcaaggtg caaatgcaga
     1801 agctgctaaa gaagctggtg cagatatcgt cggtttcgaa gatttggctg ctgaaatcaa
     1861 agcaggcaat ctgaactttg atgtcgttat tgcttctccc gatgcaatgc gtattgttgg
     1921 tcagttgggt actattttgg gtcctcgagg cttgatgcca aaccctaaag taggtacggt
     1981 tactcctaac gttgctgaag cagttaagaa tgcaaaagca ggtcaagtac aataccgtac
     2041 agataaagca ggtatcgttc atgcaacgat tggtcgtgct tctttcgctg aagctgattt
     2101 gaaagagaac tttgatgcgt tgctggatgc tatcgttaaa gccaagcctg ctgccgctaa
     2161 aggtcagtat ctgaaaaaag ttgctgtgtc tagcaccatg ggtttgggta ttcgcgttga
     2221 tacatcaagc gtaaataact aatcttaagg aattttcaag cagtttggtt ttctgggctg
     2281 cttgaatttg ggctacttaa aattaagtag atgtccaaga ccgtagggat cgtaagattt
     2341 aatcgtaact gccctacgca gacggtagtc ctgaaacaca ttgcaagatt gcttgtaaga
     2401 tgtcttttta ggttaccgcg ctggtgggat atcgttttgg tatcctgttt ataaacagtg
     2461 ggaggtagac cttgagtctc aatattgaaa ccaagaaagt ggcggtcgag gaaattagcg
     2521 cggcaattgc taatgctcaa accctcgtag tcgctgaata tcgcggtatc agtgtttcca
     2581 gtatgactga gcttcgtgcg aatgcacgta aagaaggcgt ttatttgcgc gttctgaaaa
     2641 atactttggc tcgtcgtgca gtgcaaggta cttcatttgc agaattggcc gatcaaatgg
     2701 ttggtccgtt ggtttacgct gcttctgaag atgctgttgc tgctgctaaa gtgttgcacc
     2761 aattcgcgaa aaaagatgac aaaattgtcg ttaaagccgg ttcttacaat ggcgaagtaa
     2821 tgaatgctgc tcaggttgct gagttggctt ctattccgag ccgcgaagag ctgttgtcca
     2881 aactgttgtt cgttatgcaa gctcctgtat cgggctttgc gcgcggtttg gctgctttgg
     2941 cagagaaaaa agccggcgaa gaagccgctt aatcgatttt gtttctgtta atcaattatt
     3001 ttttaataca atatttggag taaaatagca tggctattac taaagaagac attttggaag
     3061 cagttggttc tttgaccgta atggaattga acgacttggt taaagctttt gaagaaaaat
     3121 tcggtgtttc tgctgctgct gttgcagttg caggtcctgc tggtgccggt gctgccgatg
     3181 ctgaagaaaa aaccgaattt gatgtcgttt tggcttctgc cggcgatcaa aaagtcggcg
     3241 tgattaaagt tgtccgtgca attaccggtt tgggtctgaa agaagctaaa gacatcgttg
     3301 acggcgcacc taaaaccatt aaagagggtg tttctaaagc tgaagccgaa gacatccaaa
     3361 aacaactgga agaagcaggc gctaaagtcg aaatcaaata atttgatgct tcttatgaag
     3421 gctggcagtt ttctgccagc cttattttgc ttcttaaaat aaacatcaag tattgtttac
     3481 atttatttgc atagttttta tcaagtcatt gcaaataaat gtaaatatca gattgatgcg
     3541 taccgttgtt tcagacggcc tattattgaa aattactttt cggagtgtgt atgaactatt
     3601 cgtttaccga gaaaaaacgt atccgtaaga gttttgcaaa gcgggaaaat gttttggaag
     3661 ttcctttctt gctagcaacc caaattgatt cttatgcgaa gtttttgcag ctggaaaatg
     3721 cttttgacaa acgtaccgat gacggtctgc aggcggcatt taattctatt ttcccgattg
     3781 tgagccataa cggttatgcg cgattggagt ttgtgcatta cacattgggc gagcctttgt
     3841 tcgatattcc cgaatgtcag ttgcgcggaa tcacttatgc agcccccttg cgcgcgcgta
     3901 tccgtttggt gattttggat aaggaagcat ctaaaccgac ggtaaaagaa gttcgtgaaa
     3961 acgaagtgta tatgggcgaa attccgttga tgaccccgag cggttctttt gtgattaacg
     4021 gcacagagcg tgtgattgtc tcccagttgc accgttcgcc cggcgtattc ttcgagcatg
     4081 acaaaggtaa gacgcactct tccggcaaat tgttattctc cgcccgcatc attccctacc
     4141 gtggttcatg gttggatttt gaatttgatc cgaaagattt gctgtatttc cgtatcgacc
     4201 gccgccgtaa aatgccggta acgattttgt tgaaggcttt aggctacaac aatgagcaaa
     4261 tcttggatat tttctacgac aaagaaacgt tctatttgtc ttcaaacggt gttcaaaccg
     4321 atttggttgc agaccgtctg aaaggcgaaa ctgccaaggt cgatatcttg gataaagaag
     4381 gcaatgtatt ggttgccaaa ggtaagcgca ttactgcgaa aaatatccgt gatattacca
     4441 atgcaggcct gacccgtttg gatgtagaac cggaaagcct gctgggcaaa gcattggctg
     4501 ccgatctgat tgattcggaa accggcgagg tattggcttc tgccaatgat gaaattacag
     4561 aagagttgtt ggccaaattt gatatcaacg gcgtaaaaga aattacgacc ctttatatca
     4621 atgagctgga tcagggtgct tatatctcca ataccttgcg tacggatgag actgccggcc
     4681 ggcaggcggc tcgtgttgcg atttaccgta tgatgcgtcc gggcgaaccg cccaccgaag
     4741 aggcggtcga gcaattgttt aaccgcttgt tcttcagtga agacagctac gatctgtccc
     4801 gcgtaggccg tatgaaattt aatacgcgca catacgaaca aaaactgtcc gaagcccaac
     4861 aaaactcttg gtacggccgc ctgctgaacg aaacgtttgc cggtgctgcc gacaaaggcg
     4921 gttatgtcct gagcgtcgaa gatattgtcg cctcgattgc gactttggtc gagttgcgta
     4981 acggccatgg cgaagtggac gatatcgatc acttgggcaa ccgccgagta cgttcggtag
     5041 gcgagctgac tgaaaaccaa ttccgtagcg gtttggcccg tgtggaacgt gccgtaaaag
     5101 aacgtttgaa tcaggcggaa tcagaaaact tgatgccgca cgatttgatt aatgcaaaac
     5161 ctgtttctgc cgctattaaa gaattcttcg gctccagcca attgagtcag tttatggatc
     5221 agaccaaccc cttgtctgaa gtaacccata aacgccgtgt atctgcattg ggtccgggcg
     5281 gtttgacccg cgaacgtgca ggatttgagg tgcgggacgt gcatccgacc cactacggtc
     5341 gcgtatgtcc gattgaaacg cctgaaggtc cgaacatcgg tttgatcaac tcattgtccg
     5401 tttatgcgcg caccaatgat tacggtttct tggaaacgcc ttaccgccgc gttatcgacg
     5461 gcaaagtaac cgaggaaatc gattacttgt ctgccatcga agaaggccgc tatgtgattg
     5521 cacaggcgaa tgccgatttg gattcagatg gcaatctgat tggcgatttg gttacctgtc
     5581 gtgaaaaagg cgaaaccatt atggcaacgc ccgaccgcgt ccaatatatg gacgtggcaa
     5641 ctggtcaagt ggtatccgtt gcagcatccc tgattccatt cttggaacat gatgacgcga
     5701 accgcgcatt gatgggtgcc aacatgcaac gtcaggcagt gccttgcttg cgtcctgaaa
     5761 aaccgatggt cggtaccggt atcgagcgtt ccgttgccgt tgactctgct actgcaatcg
     5821 ttgcccgccg aggcggcgtg gtcgagtatg tcgatgccaa ccgcgttgtg atccgtgtcc
     5881 atgacgacga agcgactgcc ggtgaagtgg gtgtcgatat ttacaacttg gttaaattca
     5941 cccgttccaa ccagtctacc aatatcaatc agcgtcctgc cgtcaaagcc ggcgatgttt
     6001 tgcaacgcgg cgatttggtg gccgacggcg cgtccaccga ttttggcgaa ttggctttgg
     6061 gtcaaaatat gaccatcgcc ttcatgccgt ggaacggtta caactacgaa gactcgattc
     6121 tgatttccga aaaagtggct gcggacgacc gctatacttc gattcacatt gaggaattga
     6181 atgtcgttgc ccgcgatact aagctgggtg cggaagacat tacccgcgat attccgaact
     6241 tgtccgagcg tatgcaaaac cgtttggacg aatccggtat cgtttacatc ggtgcggaag
     6301 tagaagccgg cgatgtgttg gtaggcaagg taacgcctaa aggcgaaacc caactgacgc
     6361 cggaagaaaa actgctgcgc gccatcttcg gtgaaaaagc atctgacgta aaagatactt
     6421 cattgcgtat gcctaccggc atgagcggta ccgttatcga cgttcaagtc ttcactcgtg
     6481 aaggtattca acgcgacaaa cgtgctcaat ccattatcga ttccgaattg aaacgctacc
     6541 gtttggattt gaacgaccaa ttgcgtattt tcgacaacga cgcattcgac cgtatcgagc
     6601 gtatgattgt cggtcagaaa gccaacggtg gtccgatgaa gctggccaaa ggcagcgaaa
     6661 tcacgaccga atatctggcg ggtctgccga gcaggcacga ttggttcgat atccgtctga
     6721 ccgatgaaga tttggccaag cagttggaac tgattaaagt gagcctgcaa caaaaacgcg
     6781 aagaagcgga cgagttatac gaaatcaaga agaaaaaact gacccaaggc gacgaattgc
     6841 aacccggcgt acaaaaaatg gtgaaagttt ttatcgccat caaacgccgt ctgcaagccg
     6901 gcgacaaaat ggcgggccgc cacggtaaca aaggcgtggt atcgcgcatt ctgccagtgg
     6961 aagacatgcc ttacatggcg gacggccgtc cggtagacat cgtactgaac ccattgggcg
     7021 taccttcccg tatgaacatc ggtcagattt tggaagttca cttgggttgg gcagcaaaag
     7081 gtatcggcga gcgtatcgac cgtatgctga aagagcaacg caaagcaggc gagttgcgcg
     7141 agttcttgaa cagactctac aacggcagcg gtaagaaaga agatttggat gccctgactg
     7201 atgaagaaat catcgaactg gcctccaacc tgcgcaaagg tgcatctttc gcctctcctg
     7261 tattcgacgg tgcgaaagag tctgaaatcc gcgaaatgct gaacttggct tatccgagcg
     7321 acgatcctga ggttgaaaaa ctgggcttca acgacagtaa aacccaaatc acgctgtatg
     7381 acggccgttc aggcgaagca tttgaccgca aggttacagt aggtgtgatg cactatctga
     7441 aactgcacca cttggttgac gaaaaaatgc acgcgcgttc taccggtccg tacagtctgg
     7501 ttacccagca gcctttgggc ggtaaagccc agttcggcgg ccaacgtttc ggcgagatgg
     7561 aggtttgggc attggaagca tacggcgcgg catacacgct gcaagagatg ctgactgtga
     7621 agtctgacga cgtgaacggc cgtaccaaaa tgtacgaaaa catcgtcaaa ggcgaacaca
     7681 aaatcgatgc cggtatgccc gagtccttca acgtattggt caaagagatt cgctcactgg
     7741 gcttggatat cgatttggaa cgttactaaa caaaagtttt cagacggcct ttcagggtcg
     7801 tctgaaaaag tggtttcaga ataagaatga agcaatcggc atttaggccg tctgaaatca
     7861 aaagtaccgt ttcccaatat cgaaaatccg ccatgcggta aaaatacttc cttcaaggag
     7921 caaaaatgaa tttgttgaac ttatttaatc cgttgcaaac tgccggcatg gaagaagagt
     7981 ttgatgccat taaaatcggt attgcctctc ccgaaaccat ccgctcatgg tcttatggcg
     8041 aagtcaaaaa acctgaaacc atcaactacc gtacgttcaa acctgagcgt gacggtttgt
     8101 tctgtgccaa aatctttggc ccggtcaaag actacgaatg cttgtgcgga aaatacaaac
     8161 gcttgaaatt taaaggcgta acgtgtgaaa aatgcggcgt ggaagtaacc ctgtccaaag
     8221 tgcgccgcga acgcatgggt catatcgaat tggctgcgcc cgtcgcacat atttggttct
     8281 taaaatccct gccttcccgc ttgggtatgg tgttagacat gactttgcgc gacatcgagc
     8341 gcgtattgta ctttgaagca tttgtggtaa ccgatcccgg tatgactccg ctgcaacgcc
     8401 gccaattgct gactgaagac gattactaca acaagctgga cgaatacggc gacgatttcg
     8461 atgccaaaat gggtgcggaa ggtatccgcg aattgctgcg taccctgaat gtagcgggcg
     8521 aaatcgaaat cctgcgccaa gagttggaat cgaccggttc cgacaccaaa atcaaaaaaa
     8581 tcgccaaacg cttgaaagta ttggaagcct tccatcgttc cggtatgaaa ctggaatgga
     8641 tgattatgga tgtgctgccg gtattgccgc ctgatttgcg tccgttggtt ccattggatg
     8701 gtggtcgttt tgccacttcc gatttgaacg atttgtaccg ccgcgttatt aaccgtaaca
     8761 accgtctgaa acgtctgttg gaactgcatg cgcctgacat catcgtccgc aacgaaaaac
     8821 gtatgttgca agaagcagtt gactcgctgt tggataacgg ccgtcgcggt aaagccatga
     8881 ccggcgccaa caaacgcccg ctgaaatcat tggcagacat gattaaaggt aaaggcggtc
     8941 gcttccgtca aaacctgttg ggcaaacgtg tggactactc cggccgttcc gtgattaccg
     9001 taggcccgta cctgcgtctg caccaatgcg gtttgccgaa aaaaatggct ttggaactgt
     9061 tcaaaccgtt cattttccac aaattggaaa aacaaggttt ggcctctacc gttaaagcag
     9121 cgaaaaaatt ggtagagcaa gaagtaccgg aagtatggga catcttggaa gaagtcatcc
     9181 gcgaacatcc gattatgctg aaccgtgcgc cgaccctgca ccgtttgggt attcaagcgt
     9241 tcgaacctat cttgattgaa ggtaaagcga ttcagttgca cccattggtg tgtgctgcgt
     9301 tcaacgccga ctttgacggc gaccaaatgg cggtacacgt tccattgagc ttggaagcac
     9361 aaatggaagc acgcacgctg atgctggctt caaacaacgt attgtctccg gccaacggcg
     9421 aaccgattat cgtaccttcc caagacatcg tattgggcct gtactatatg actcgcgatc
     9481 gtatcaatgc caaaggcgaa ggcagcctgt ttgccgatgt gaaagaagtg catcgcgcat
     9541 accataccaa acaggtcgag ctgggtacga aaatcaccgt acgtctgcgc gaatgggtga
     9601 aaaacgaagc aggtgagttt gagcctgtcg ttaaccgtta cgaaacaacc gtcggccgtg
     9661 cattgttgag cgaaatcctg ccgaaaggcc tgccgtttga atatgtcaac aaagcgttga
     9721 agaaaaaaga aatttctaaa ctgattaacg catcgttccg cctgtgcggc ttgcgcgata
     9781 cggttatctt tgctgaccac ctgatgtaca ccggtttcgg atttgcggca aaaggcggta
     9841 tttccattgc cgttgacgat atggaaattc caaaagaaaa agcggccttg ctggctgaag
     9901 ccaatgccga ggttaaagaa atcgaagacc aataccgtca aggtttggtt accaacggcg
     9961 aacgctacaa caaggtggtc gatatttggg gtcgtgccgg cgataagatt gctaaagcga
    10021 tgatggacaa cttgtccaaa caaaaagtta tcgaccgtgc cggcaacgaa gtcgatcaag
    10081 agtcattcaa ctccatttat atgatggcgg actccggtgc ccgtggttct gcagctcaga
    10141 ttaaacagtt gtccggtatg cgtggcttga tggcaaaacc tgacggctcg attattgaaa
    10201 cgccgattac ctcaaacttc cgtgaaggtc tgaccgtatt gcaatacttt attgcgaccc
    10261 acggtgcgcg taagggtttg gcggataccg cattgaaaac cgcgaactcc ggttacctga
    10321 ctcgtcgtct ggtagacgta actcaagatt tggtcgttgt tgaagacgat tgcggtactt
    10381 cagacggctt tgtcatgaag gcagtggtac aaggcggtga tgtgattgaa gcattgcgcg
    10441 atcgtatttt gggtcgtgtt accgcgtctg acgttgtcga tccgtcaagt ggcgaaacct
    10501 tggttgaagc cggtacgttg ctgactgaaa aactggtgga tatgatcgac caatccggtg
    10561 tcgatgaagt caaagtccgt acgccgatta cttgtaaaac ccgtcacggc ctgtgtgcac
    10621 actgttacgg tcgtgacttg gcacgcggca aactggttaa cgccggtgag gcagtcggtg
    10681 tgattgctgc acaatccatt ggcgaaccgg gtacccagtt gaccatgcgt acgttccaca
    10741 tcggtggtgc ggcatcccgt gcggcagcag ccagccaagt ggaagccaaa tccaacggta
    10801 cggcacgatt cagcagccag atgcgctacg ttgccaacaa caaaggcgag ttggttgtca
    10861 tcggccgttc ttgtgaagtc gtgattcacg acgatatcgg ccgtgaacgc gaacgccaca
    10921 aagtacctta cggtgccatc ctgctggtac aagacggtat ggccattaaa gccggtcaaa
    10981 ccttggcaac ctgggatccg catacccgtc cgatgattac cgaacacgca ggtatggtga
    11041 aattcgaaaa cgtggaagag ggcgttaccg ttgccaaaca aaccgatgat gtaaccggtt
    11101 tgtccacttt ggtggtgatt gacggtaaac gtcgttcctc tagtgcttcc aaactgctgc
    11161 gtccgactgt gaaactcttg gacgaaaacg gcgtggaaat ctgtattccc ggtacttcta
    11221 ctccggtatc catggcattc cccgttggtg cggtgattac cgtacgcgaa ggtcaggaaa
    11281 tcggtaaagg cgacgtattg gcgcgtattc cgcaagcctc ttccaaaacc cgcgacatta
    11341 ccggcggcct gccgcgcgtt gccgaattgt ttgaagcacg cgtgccgaaa gatgccggta
    11401 tgttggcgga aattaccggt accgtttcct tcggcaaaga gaccaaaggc aagcaacgtc
    11461 tgattgttac tgacgtggac ggtgtagcat acgagacctt gatttccaaa gagaaacaaa
    11521 ttctggtaca cgacggtcaa gtggtaaacc gcggtgaaac catcgtggac ggcgcggtcg
    11581 atccgcacga tattctgcgt ttgcaaggta tcgaagcact ggcacgctac attgtccaag
    11641 aggtgcaaga ggtttaccgt ctgcaaggtg tgaagatttc tgataaacac atcgaagtca
    11701 tcatccgtca aatgttgcgc cgtgtgaaca ttgcggatgc cggcgaaacc gggttcatta
    11761 ccggagagca ggtcgaacgc ggcgatgtga tggcggccaa tgaaaaagct ttggaagaag
    11821 gcaaagaacc ggcgcgttac gaaaacgtat tgctgggtat taccaaagct tccctgtcca
    11881 ccgacagctt catttctgcc gcatcgttcc aagaaacgac ccgcgttctg accgaagccg
    11941 cgattatggg caaacaagac gagttgcgtg gtttgaaaga aaacgtcatc gtcggtcgct
    12001 tgattcctgc cggcaccggt ttgacttacc accgcagccg tcatcaacaa tggcaagagg
    12061 tggaacagga gactgccgaa acccaagtaa cggatgaata atctttggtg catccattca
    12121 ataaaaaacc gcaagccttg agcttgcggt ttttctttgt ccgattaagg caaaaacaag
    12181 cgttttcgtc attttgaggc gtgtggatta ttccttaggt attttcgggc cggagaccaa
    12241 cgaggtggcg ggtgtcgtcg gtacgtccgg agaccaaaat aactttgcca gggatgttgg
    12301 tttcggcggt caaaaaaagt agcgtcttaa tgttttccat ttaaacaaat gtcgtctgaa
    12361 acttcagacg gcatttcctt taagaaataa atatgaaacc cagaaatctc ttttttgcag
    12421 gctgcctgct gacttcggcg acgtttgccg aggatatcgg cgtacctgtc gaactgatta
    12481 acgtcggtaa tcggattgcg atgccgtctg aaggggaaag cctcgccctc ctgccgtttg
    12541 ccgaggatgt accgccggtt cgcgatgcaa tgccgtctga agttcctaaa agcgcggcag
    12601 gcggcgatgt tcggggtgac cggatgagaa tgccgattaa catcggatga gcgcggcttt
    12661 atggcataaa aaactgtcgt ggaaaggatt tacaccccaa ataaatttcc gttacaacaa
    12721 gatcaacagc aatatgcccg ccttttattc gcgcagcggc aaggaacggt ttgtcagtat
    12781 agaaaaaacg tattgacagt attttcttca gtcgtccgac tgattgtgag ggatgtcggt
    12841 aaatatttat cggcaaacaa gaaaatcatc tttcttcttg tcgttatgct tgactgtctg
    12901 cttgcaataa aaatataatt ccactcttgc cgacatggtg tcggcaagta tttaactcaa
    12961 caggacgaga aaatatgcca actatcaacc aattagtacg caaaggccgt caaaagcccg
    13021 tgtacgtaaa caaagtgccc gcactggaag cttgcccgca aaaacgtggc gtgtgcaccc
    13081 gtgtatacac aactacccct aaaaaaccta actctgcatt gcgtaaagta tgtaaagtcc
    13141 gcctgaccaa cggttttgaa gtcatttcat acatcggcgg cgaaggtcac aacctgcaag
    13201 agcacagtgt cgtattgatt cgcggcggtc gtgtaaaaga cttgccaggt gtgcgttacc
    13261 acactgtacg cggttctttg gatactgcag gtgttaaaga ccgtaaacaa gcccgttcca
    13321 aatacggtgc taagcgtcct aaataattac tgggacttaa ataggcacgt cggccgccta
    13381 agctgaacaa cggccgagta agtgaatact caattgggta ttcatgggaa tagacccgac
    13441 tgaatagatt aaaggaaatt aaaatgccaa gacgtagaga agtccccaag cgcgacgtac
    13501 tgccagatcc taaattcggc agcgtcgagt tgaccaaatt catgaacgta ttgatgattg
    13561 acggtaaaaa atccgttgcc gagcgtatcg tttacggtgc gttggaacag attgagaaaa
    13621 aaaccggcaa agtagcaatc gaagtattta acgaagccat tgcaaacgcc aaacctatcg
    13681 tggaagtgaa aagccgccgt gtaggtggtg caaactacca agttcctgtt gaagttcgtc
    13741 cttcacgccg tttggctttg gcaatgcgct gggttcgcga tgcggcccgc aaacgtggtg
    13801 agaaatccat ggacctgcgt ttggcaggcg aattgattga tgcgtccgaa ggccgtggcg
    13861 gtgcgttgaa aaaacgtgaa gaagtacacc gtatggctga agccaacaaa gcattctctc
    13921 acttccgttt ctaattttga aaggctaata aaatggctcg taagaccccg atcagcctgt
    13981 accgtaacat cggtatttcc gcccatattg acgcgggtaa aaccacgacg acagaacgta
    14041 ttttgttcta taccggtttg acccacaagc tgggcgaagt gcatgacggt gcggctacta
    14101 ccgactacat ggaacaagag caagagcgcg gtattaccat tacctccgct gccgttactt
    14161 cctactggtc cggtatggcg aaacaattcc ccgagcaccg cttcaacatc atcgacaccc
    14221 cgggacacgt tgactttacc gtagaggtag agcgttctat gcgtgtattg gacggcgcgg
    14281 taatggttta ctgcgcggtg ggcggtgttc aaccccaatc tgaaaccgta tggcggcaag
    14341 ccaacaaata ccaagtgccg cgcttggcgt ttgtcaataa aatggaccgt cagggtgcca
    14401 acttcttccg tgttgtcgag caaatgaaaa cccgtttgcg cgcaaaccct gtacctatcg
    14461 tcattccggt tggtgcggaa gacaacttca gcggtgtggt tgatttgttg aaaatgaaat
    14521 ccatcatttg gaatgaagtc gataaaggta caacctttac ctatggcgat attcctgccg
    14581 aattggtcga aactgccgaa gaatggcgtc aaaatatgat tgaagccgca gccgaagcca
    14641 gcgaagaact gatggacaaa tacttaggcg gcgacgagct gaccgaagaa gaaatcgtag
    14701 gcgcgttgcg tcaacgtact ttggcaggcg aaattcagcc tatgctgtgt ggttctgcat
    14761 ttaaaaacaa aggtgttcaa cgtatgttgg acgcagttgt agaattgctg ccagctccta
    14821 ccgatattcc tccggttcaa ggtgtcaacc cgaataccga ggaagccgac agccgtcaag
    14881 ccagcgatga agagaaattc tctgcattgg cgttcaaaat gttgaacgac aaatacgtcg
    14941 gtcagctgac ctttatccgc gtttactcag gcgtagtaaa atccggcgat accgtattga
    15001 actccgtaaa aggcactcgc gaacgtatcg gtcgtttggt acaaatgact gccgcagacc
    15061 gtactgaaat cgaagaagta cgcgccggcg acatcgcagc cgctattggt ctgaaagacg
    15121 ttactaccgg tgaaaccttg tgtgcggaaa gcgcgccgat tatcttggaa cgtatggaat
    15181 tccccgagcc ggtaatccat attgccgttg agccgaaaac caaagccgac caagagaaaa
    15241 tgggtatcgc cctgaaccgc ttggctaaag aagacccttc tttccgtgtc cgtacagacg
    15301 aagaatccgg tcaaaccatt atttccggta tgggtgagct gcacttggaa attattgttg
    15361 accgtatgaa acgcgaattc ggtgtggaag caaatatcgg tgcgcctcaa gtggcttacc
    15421 gtgaaactat ccgcaaagcc gttaaagccg aatacaaaca tgcaaaacaa tccggtggta
    15481 aaggtcaata cggtcacgtt gtgattgaaa tggaacctat ggaaccgggt ggtgaaggtt
    15541 acgagtttat cgatgaaatt aaaggtggtg tgattcctcg cgaatttatt ccgtctgtcg
    15601 ataaaggtat ccgcgatacg ttgcctaacg gtatcgttgc cggctatcct gtagttgacg
    15661 tacgtatccg tctggtattc ggttcttacc atgatgtcga ctcttcccaa ttggcatttg
    15721 aattggctgc ttctcaagcg tttaaagaag gtatgcgtca agcatctcct gccctgcttg
    15781 agccaatcat ggcagttgaa gtggaaaccc cggaagaata catgggcgac gtaatgggcg
    15841 acttgaaccg ccgtcgcggt gttgtattgg gtatggatga tgacggtatc ggcggtaaaa
    15901 aagtccgtgc cgaagtacct ttggcagaaa tgttcggtta ctcgaccgac ctgcgttctg
    15961 caacccaagg ccgcgctact tactctatgg agttcaagaa atattctgaa gctcctgccc
    16021 acatagctgc tgctgtaact gaagcccgta aaggctaatc agaaaaggcc gtctgaaact
    16081 gaaaataaat tttcagacgg ccattgttct ttaatcgatc tttatatgta aaggaattag
    16141 ctcatggcta aggaaaaatt tgaacgtagc aaaccgcacg taaacgttgg caccatcggt
    16201 cacgttgacc atggtaaaac cactctgact gctgctttga ctactatttt gtctaaaaaa
    16261 ttcggtggcg ctgcaaaagc ttatgaccaa atcgacaacg ctcctgaaga aaaagctcgt
    16321 ggtattacca ttaatacctc acacgtagaa tacgaaactg aaacccg
//
LOCUS       DAWGRZ010000030        16173 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000030 DAWGRZ010000000
VERSION     DAWGRZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16173
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.030"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(46..1227)
                     /gene="odhB"
                     /locus_tag="WHT44_000670"
     CDS             complement(46..1227)
                     /gene="odhB"
                     /locus_tag="WHT44_000670"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ2532890.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARVDTAATAAAEAPAAAPAEAAPAAAPAA
                     TQNNAAMPAAAKLAAETGVDVNALQGSGRDGRVLKEDVQNAAAKPAGAAAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLD
                     L"
     gene            complement(1327..4155)
                     /locus_tag="WHT44_000671"
     CDS             complement(1327..4155)
                     /locus_tag="WHT44_000671"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ2532891.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(4360..5643)
                     /gene="gltA"
                     /locus_tag="WHT44_000672"
     CDS             complement(4360..5643)
                     /gene="gltA"
                     /locus_tag="WHT44_000672"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ2532892.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(5755..6003)
                     /locus_tag="WHT44_000673"
     CDS             complement(5755..6003)
                     /locus_tag="WHT44_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ2532893.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(6007..6714)
                     /locus_tag="WHT44_000674"
     CDS             complement(6007..6714)
                     /locus_tag="WHT44_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ2532894.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(6834..8597)
                     /gene="sdhA"
                     /locus_tag="WHT44_000675"
     CDS             complement(6834..8597)
                     /gene="sdhA"
                     /locus_tag="WHT44_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ2532895.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYQGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPADAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDINTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(8600..8941)
                     /gene="sdhD"
                     /locus_tag="WHT44_000676"
     CDS             complement(8600..8941)
                     /gene="sdhD"
                     /locus_tag="WHT44_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ2532896.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(8935..9312)
                     /gene="sdhC"
                     /locus_tag="WHT44_000677"
     CDS             complement(8935..9312)
                     /gene="sdhC"
                     /locus_tag="WHT44_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ2532897.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(9373..9573)
                     /locus_tag="WHT44_000678"
     CDS             complement(9373..9573)
                     /locus_tag="WHT44_000678"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532898.1"
                     /translation="MPSEIFSDGILFLGMGRMLIPCREGGGKTKNLSFNLLFPRVFVK
                     IYQFVFKIHCSKWDKTGKLTFM"
     gene            complement(9590..10993)
                     /locus_tag="WHT44_000679"
     CDS             complement(9590..10993)
                     /locus_tag="WHT44_000679"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ2532899.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMRRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDDHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEAKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(11242..11979)
                     /locus_tag="WHT44_000680"
     CDS             complement(11242..11979)
                     /locus_tag="WHT44_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ2532900.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAICCVSVNDTFVMNAWSAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(12258..14534)
                     /gene="metE"
                     /locus_tag="WHT44_000681"
     CDS             complement(12258..14534)
                     /gene="metE"
                     /locus_tag="WHT44_000681"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ2532901.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADFVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGTVEFNRLSLLQKLLPVYVEILNALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLSKVNAKILLSTYFGSVAEHAALLKALPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGVIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTHKLRAELAK"
     gene            complement(14670..15548)
                     /gene="metF"
                     /locus_tag="WHT44_000682"
     CDS             complement(14670..15548)
                     /gene="metF"
                     /locus_tag="WHT44_000682"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ2532902.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            15749..16024
                     /locus_tag="WHT44_000683"
     CDS             15749..16024
                     /locus_tag="WHT44_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ2532903.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            16024..16149
                     /gene="ykgO"
                     /locus_tag="WHT44_000684"
     CDS             16024..16149
                     /gene="ykgO"
                     /locus_tag="WHT44_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ2532904.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
BASE COUNT         3954 a         4086 c         4025 g         4108 t
ORIGIN      
        1 ttcagacggc cttttcatta acaaataaaa ggccgtctga aacgattaca gatccaacaa
       61 caggcgggcc gggtcttcca acgcgtcttt aatggctacc aaggtcaata cagcttcgcg
      121 gccgtcaatg atacggtggt cgtaagacag agccagatac atcatcggac ggacaacaac
      181 ttggccgttt tcaaccacag cgcgctcttt agtggcgtgc atacccaaaa tcgcagattg
      241 aggtgggttg atgatcgggg tagacatcat agaaccgaaa gtaccgccgt tggtaatact
      301 gaatgtaccg ccggtcagat cttcgatagc gattttgccg tctttggctt ttttcgcgta
      361 atcaacaatt gcttgttcga tgtcggcaat gctcatttgg tcggcatcac gcagaattgg
      421 cacaaccaaa ccgcgtgggc tgccaattgc gataccgatg tcgaagtagc cgtggtacac
      481 gatgtctttg ccgtcaacag aagcattcac aaccgggtat tttttcaggg cggcaacagc
      541 ggctttaacg aagaaggaca taaagcccag tttcacgccg tgttctttct cgaatttttc
      601 tttgtacttc gcacgcaagt ccatgattgg tttcatgttg acttcgttga atgtagtcag
      661 aatggcgttt tcttgttgag aagccaagag gcgttctgca acacgggcac gcaggcggct
      721 cattggtacg cgttcttcag gacgtgcgcc ggcaggaagt gcaacagcag gagcagcggc
      781 tccggcaggt ttggcagcgg cattttgtac gtcttctttc aatacgcgac cgtcacggcc
      841 ggaaccttgc aatgcgttca cgtcaacacc ggtctcggca gccagtttgg cggcagcagg
      901 catagcggcg ttgttttgtg tagcagcagg agcggcagct ggggcagctt ctgcaggagc
      961 ggcggctggg gcttcagcag cggcagtagc agctgtatcg acgcgcgcca aaacttggtc
     1021 ggcaacaacg gtttcaccgt cttgagctac gatttcaacc aatacgccgg cttgtggaga
     1081 aggtacttcc aaaaccactt tgtccgtttc gatatcgatc aggatttcgt cacgggcaac
     1141 ggcttcgcca acttttttct tccattccaa gagcgtgcct tcagatacgc tttcagacaa
     1201 cataggtact tttacatcaa taatcatttt gtgtctccaa tggccctttc agacggcctg
     1261 ttgtgtgttt ttaattctgc atatgggcat tccatctgag cagactcaga cggaatgcta
     1321 cttggtttac aatgccaaag cgtcttcaac caattgtttc aattgagcaa tgtgtttgct
     1381 tgagtagccc actgcaggcg atgcgctgct tggacgaccg gcataagaca gtttttgctc
     1441 ttcgctaata acatcttcga tgcggtggcg gatttggtag aacgcgcctt ggtttttcgg
     1501 ctcttcttgt gcccaaacca cagattttgc gttcggatat ttcgccagtt cagctttaac
     1561 ctcgtcatat gggaacggat acagctgctc aacgcggaca atagcaacat catcttccag
     1621 tttacgctcg gcacggccgg cttccaagtc atagtaaacc tgaccggcac acaataccac
     1681 gcgtttcacg ctgtcgttgc ttgcgcgttc tgcggtatcg ccgataaccg gacggaaggt
     1741 cgaaccttcg gtgaagtttt ccagcgggct cattgcacct ttgaagcgca acaggcgttt
     1801 ggacatgaaa atcaccagcg gtttgcggta tgaacccaag acttgacgtt gcaagaggtg
     1861 gaacatttgc gacgcttcag acggcataat gacttgcata ttgttctcag aacacagttg
     1921 caaccaacgt tctacgcgtg cagaagagtg ctcggggcct tgaccgtcgt agccgtgcgg
     1981 caggatggta gtcagaccgc acaaacgacc ccacttggtt tcgcctgaag acaggaattg
     2041 gtcaatagtc acttgcgcgc cgttggcgaa gtcaccgaat tgagcttccc aaatggtcag
     2101 tttgtcaggt gcggagcagg caaagccgta ctcgaacgcc atcacggctt cttcgttcaa
     2161 aatggagtcg ataaccagga actcgcccat gccttcgccc atatggcgca gaggaacata
     2221 agtaccgtcg tcccattttt cgcgtttttg atcgtgcaat acggcgtggc ggtgcgagaa
     2281 cgtgccgcgt cccgagtcct cgccggaaat acgcacgccg tgacctttgg ttacgaggct
     2341 ggcgtatgcc aaggtttcgg ccatacccca atctatggcc tgtttgccgg atgccatggc
     2401 tttacgcgct tcaatcacac gttttgcagt cggatgcagg gcaaagcctt ccggtacggc
     2461 ggtaaacttc tcagtgagac gttcaatatc tgcggcaggc aaaccggttt cgatgtgttc
     2521 gcgccaatct ttgccttggt atttgctcca gtcgatttgc gtgcgttgga agttgctcaa
     2581 cgttgtttgc tcgacgtgct cgcctttgtc caaagcatca cggtaagctt ggatgtaacc
     2641 gtcagcctcg gcttgggtta ccacgccttc ggcaatcagt tgctcggtgt acaaagcacg
     2701 cgcaccgggg tgttgcgata cttttttgta catcatcggt tgggtcaagg tcggatcatc
     2761 gccctcgttg tgaccccatt tacggtagca gacaacgtca atcacgatgt ctttatggaa
     2821 ttttttgcgg taatccaaag cggcttggat agcaaagcaa acgcgttcgg gatcatcgcc
     2881 gttcacatgg ataaccgggg cggaaaccat ttttgcgata tcggtacagt gtacggttga
     2941 acgggtatcg cggatatcgg aagtggtaaa gccgatttgg ttgttgatga cgatatgaac
     3001 cgtaccgccg gtggtataac cgcgcgtttt agacaggttg aatgtcgctt ggttgactcc
     3061 cagaccgata aatgcggagt cgccgtgaat caataccggc aagactttgt cgcggccgtt
     3121 ttcgcccaaa cgtttttgtt tggcgcgcgc agaaccttcc accaccgggt tgacgatttc
     3181 caagtgtgac gggttgaacg ccaaagaaac gtgcatcggg ccgtgcggcg tggcaatatc
     3241 ggagctgaag cccatatggt atttcacgtc gccgctgggc agtttgattt cggcacgacc
     3301 ttcaaattcg gcaaacaaat cgccgggttt tttgcccaaa atgttcacca aaacattcag
     3361 acggccacgg tgcgccatac cgatgatgac ctcttccaca ccgtctttac cggcgttttg
     3421 aatcaggtag ttcaaaccgg caatcgcgct ttcgccgcct tcgacaccga aacgtttctg
     3481 accgacatat ttggtatgca gataacgttc caaagtctcg gcagcagtca tctctttcaa
     3541 gatacggcgt ttttgatcgg cattgtaatg cggtgtggac aatacgcttt caaaataatt
     3601 gcgtacccag cggcgctctt cggtattggg aatatagata tattccaatg cgatgtggcc
     3661 gcagtaggtt tgtttgaggt tgctgatgat ttgggacaaa ggcagtttgc cgcgattggc
     3721 aaaatcgccc tcgcccatat tgaattgaag cgccatatcg gcatctgaca gaccgtggaa
     3781 tttcggatcg agggcttcaa tatcgcgcgg ggggatacgt ttgagcggat caagttgggc
     3841 tgcacccacg ccttggatac gataggcgga aatcagccgt aaaacgctga cttgcttttt
     3901 cagcattgcc tcatccgcac cgcccgcaac ggcagatgca attttctttt tcgccaaagt
     3961 aacaaatgat tcgcgaatcg gtgtgtgtgc gacatcgaca gcaaccgtcc ccggctgttt
     4021 gctcaaatcg gtgaaatact gcttccattt ttcatcaacc gcatcggggt tttccaaaaa
     4081 agcctcgtac aattcctcaa tgtaaggtgc gtttgaaccg aacaggtaag agaaattgag
     4141 tttttcgtcc atcatggcgt gcgctctttt ctttaaaata taaacaaagg gtagaagtcc
     4201 gcgtcctgac ggctaaactg atgccgcctg aaaagacatt ttattctacc ctattttaaa
     4261 ccggattgct tgtcttgatt tcatccgact acaatcagac ggattcggga aacaatccgg
     4321 ttctctgata ttgcctgttt gacaatatat taatgtatgt tacctctcgc ctggcggcac
     4381 ataatcgcgg cgttccgaac cggtataaag ctggcgcggg cggccgattt tcagcgaagg
     4441 atcgctaatc atctcgtgcc agtgcgaaat ccagcccacg ctgcgcgaca gggcgaagat
     4501 gacggtaaac atttcggtcg ggatgcccag cgcggacagg acgatgccgg aatagaaatc
     4561 gacgtttgga tacagtttgc gttcgataaa gaacgggtct ttcagcgcaa tctgttccaa
     4621 ttccatcgcc agtttgaatt tcggactgtc ttccaagccc aattccttca aaacttcata
     4681 gcaggtttcg cgcataatgc tggcacgcgg atccatattg cggtacacgc ggtgaccgaa
     4741 gcccatcaga cggtatttgc gttgtttcac accttccatg tatgcggcaa cattagacac
     4801 atcgccgatt tcgtccaaca ttttcaacac ggcttcgttc gcaccgccgt gcgaagcacc
     4861 ccacaggcag gcgataccgg cagcaataca ggcaaacggg ttcgcacccg aagaccctgc
     4921 cagacggacg gttgaagttg aggcgttttg ctcgtggtcg gcatgcaaaa taaagatgcg
     4981 gtcgagcgcg cgtgccaaaa cgggattggg tttgtagtct tcacacggcg tggcgaacat
     5041 catatgaagg aagttttcgg aataagaaag attattcttc ggataattga acggcagacc
     5101 gtttgaatag cggtagcaca ttgccgcaat ggtcgggatt ttagaaatca ggcggtaaat
     5161 cgcgattttg cggtgttcgg gattgctaat gtccaagctg tcttggtaga acgcagacag
     5221 tgcgccgacc acgccgacca tcatcgccat cggatgcgcg tcgcggcgga acccccggaa
     5281 gaaccaagtc agctgttcat gcaccatcgt gtggcggcgg acggtattgt caaattctgc
     5341 cttttgctcg ggagtcggca gttcgccgta aatcaacagg tagcagactt ccaaataatc
     5401 ggacttttcg gccagctgct cgatggggta tccgcgataa taaagcaagc cttgatcgcc
     5461 gtcgatgtaa gtaattttag actcacagct tgcggttgaa acaaatccgg ggtcgaagga
     5521 aaacaaacct gtattttttg tcagcccccg aatgtcaacc acatcgtgcc cgatgctggc
     5581 ttccaatacc ggcagctcca aacctgcctg acccggtacg ttgagtttga ttgatttgga
     5641 catattattg ctcctttgca agttataacc cacgttactg catccgcagc acgttctttg
     5701 ccttcagacg gcttttgcat tttgaaatct gcctgcaggc agatttcaga tgcctcatgc
     5761 ccgtctgatt ttttcaagca tcgggataag gtgccctttg tccgtttccg aatgcccgtt
     5821 aatcaaggca agcaattctt gatcttgaaa ttcaaggatt tcggaaaact cggacagctc
     5881 tttatcgctc aaatgctcga attctttttc cataaacctg ccgaagatta aatctaattc
     5941 caacaatccc cggcgggttt gaaaacggat tttccgtttg gcaatatcgt caaaaaccat
     6001 catttcttaa acggcccgtt tcaacataat ctctttaatc ttaccgatgg ctcgggtcgg
     6061 attcaagtgt ttaggacata cgtctacgca gttcataatg gtgtggcaac ggaacaaacg
     6121 gtatgggtcg ttcagattat ccaaacgctc attagtgatg gtatcacggc tgtccgcaat
     6181 gaaacggtaa gcattcagca aaccggacgg accgacgaat ttatcagggt tccaccaaaa
     6241 tgacgggcag gcagtcgaac agcaggcgca caaaatacac tcgtacaaac cgtccaactc
     6301 tttacgctct tcctgagttt gcagacgctc tttgtccgca tcaatcggat tatcgttgac
     6361 aacataaggt ttgacggaat ggtattgttt gaagaactgg gtcatatcca caatcaggtc
     6421 gcggataaca ggcagacctg gcagaggacg gattttaact ggctgtttca agccacgcag
     6481 atcggtcaaa cacgccaagc cgtttttgcc gttgatgttc ataccgtccg atccgcaaat
     6541 gccttcgcgg caggagcggc ggaaagacaa ggtatcgtct tgtgctttca ggcgtaccaa
     6601 agcatccaaa agtttcacgt cggtcggttc caattccaac tcgtaacgct gcatataagg
     6661 cttggcatca acatccgggt tgtaacggta aatttcaaaa ctcatttttt ccatgagaag
     6721 tgttcctttt ctcatcaact gcccaatgat tttgtattat aaattcaagc agttcaatgt
     6781 gggtatggtt agatttcaga cgacctttga ggcgaagact gtctgaaaac gcatcaataa
     6841 acgcgcttgg ccggtttgat gtattccacg ctcaaaggct tggtgtgcac cggtttgtag
     6901 gacaaggtat tgatatctga atggtacagc gtatgtttca tccagttttc atcatcgcgc
     6961 tcaggatggt cgtctgaagc gtgcgcaccg cgtgattctt tacgtgcttc ggcagacacc
     7021 aaagtcgctt tcgccacttc aatcaggtta tccaattcca aagcctcgat acgcgcggta
     7081 ttccacactt tgctcttgtc tttgatttcg gtacgtttca cacgctcggc aatcgccatg
     7141 acttctcgaa cgcctttgct cagaatctca tcagtacgga acacgccggc gtgcagttgt
     7201 acagagcgtt gcagttcgcg acgcagcgca tcgacgtttt cgccatcagt ttgattgtcc
     7261 aaacgctcga tacgttggcg ggtcagctcg cccgcatccg caggcaaagg tttccagtcg
     7321 ctttgctctt tgatgaattt aatcatgctg tcgccggcag ctttaccgaa caccaccaag
     7381 tccagcagag agttcgtacc caaacggttc gcaccgtgta cggaagcaca ggcgcactca
     7441 cctgcggcat acaggccttt tacaggcact tcgtattcgt ctccttgcgg cacaacgact
     7501 tcgccttggt aattggtcgg aatgccgccc atcatatagt gggtagtcgg cacaacagga
     7561 atcgggtctt taatcggatc gataccggcg aactgaatgg aaatctcgcg gatgcccggc
     7621 agtttttcca taattttttc tgcgccgata tggtcgattt tcagtaagac atggtctttg
     7681 tttttaccgc agccgcgacc ttcgtagatt tccatcgcca tcgcgcggga aacaacgtcg
     7741 cgagaagcca agtcttttac ggtcggcgca tagcgctcca taaagcgttc gccgtcggcg
     7801 ttcaacaaaa taccgccttc accgcgtaca ccttcggtaa tcaacacgcc tgcacctgcc
     7861 acacctgtcg ggtggaattg ccagaactcc atatcttcca acggaatacc cgcacgcgcg
     7921 caaatgccca aaccatcgcc ggtattcata taggcattgg tagaagaagc ataaatacga
     7981 ccgccaccgc cggtagcaaa catcacagct ttagcgtgga aaatataaac ttcgccggtt
     8041 tccatttcca tggcggttac gccgacgaca tcgccgtttt catcacgaat caaatcttgt
     8101 gccgtccatt ccacaaagaa ttgcgtattg gcacggacgt tttgttggta caaagtatgc
     8161 agcatcgcat gacctgtacg gtcggcaacc gcacaggcgc gttctaccgc gcgtttaccg
     8221 tgttcggcag tatggccgcc gaaaggacgc tgataaattt taccgctttc cacacggtca
     8281 aaaggcatac ccatgtgttc caactcaatt acggcttcag gcgcggcgcg gcacataaac
     8341 tcaatcgcat cttggtcgcc caaccagtcg gaacctttca cggtatcgta catgtgccag
     8401 tcccaacggt cttcctgcac attacccaaa gaggcggaaa taccgccctg cgccgctacg
     8461 gtatgcgaac gggtcgggaa cactttagac aaaacggcac aattcagacc ggatttggat
     8521 aattggaggg ctgcgcgtaa acctgcacca ccaccgccga caatcacggc atcaaacttg
     8581 cgaacaggaa aacccatact tacccccaaa ttactttaat tgaatacacc aagcagccga
     8641 ccagccagac gatggtggca acctgcaaaa acaaacgcac gccgaagggt ttgatatagt
     8701 ccatccacaa atcgcggata cccacccaag cgtgcaagaa tacggcgatg aagctcactt
     8761 gggtaaatac ttttacccaa gtttgactaa aaaatgcctg ccatgccgaa tattctttag
     8821 gcagggaaaa tagaaccact aaaagtgcaa cggtataaat caacataata accgcagtcg
     8881 cacgttgcat cgcccaatcg cgcaaaccgt aatgggcacc ggtcaattta cgttctacca
     8941 taacaacgct cccaaaacga cagtcaaaac caatgcagaa gcaaatacgg ctttagcggt
     9001 attgcgcgca gtattcagct caaggccttt gtgcgcatcc aaaaataaaa agcggatacc
     9061 ggcgagagaa tggtgcagat aagcccacaa tacaccgatt aaaaccagct tgaccaaagg
     9121 atgggaaaca atggcacggt aagtttcaaa tgcagactct tgactcaggg taccggacag
     9181 gaaatacagc aggaaaggca gcataataaa cagcccgacc ccgctgattc ggtgaaggat
     9241 ggaaactatc ccgggtatcg gcagacggat gttcggcaaa tccaaataaa caggacgcgg
     9301 tttggcagac atagtcaatt cctctgtagt tatatatcga ctcaaaaacc gtaaacccat
     9361 tgctacactg ggttacataa acgttaattt acctgtttta tcccattttg aacagtgtat
     9421 tttaaaaaca aactgataga ttttgacaaa cacgcgtgga aacagcagat taaaagaaag
     9481 atttttagtt ttgccgcccc cttcccgaca cggtatcagc atccgaccca tccccaaaaa
     9541 caaaatgccg tctgaaaaaa tttcagacgg cattgttttg ccatatcggt caggctttca
     9601 atttcgcatc ggcatcgcgc aacgcggtac gcaaaccttc ctcgataacg ggatggtaga
     9661 acggcatatc cagcatttgc ggaacggtca tcttcatttg atgtgcccaa gccaacaggt
     9721 gcgccaaatg ttcggcggcc ggacctacga tttctgcgcc gatgaagcgg ccggtggctt
     9781 tttcggcata caggcgcata tggcctttgt ttaccagcat cacgcggctg cggccttggt
     9841 ttttgaacga tacttcgccg atgacaaatt cgtcggcttg gtattgcgcg gcaacctgcg
     9901 cgtatttcaa accgacaaag ccgatttgcg gactggtgaa cactacgccg atggtgctgc
     9961 ggcgcaaacc gccgccgata ttcgggtagc ggcctgcgtt atcgccggca atcttgcctt
    10021 ggtcggcggc ttcatgcagc aggggcagtt ggttggacgc gtcgcccgca ataaagatat
    10081 gcggaatact ggtctgcata gtcagcggat cggcaacggg tacgccgcgc gcatcttttt
    10141 cgatattgat gttttccaaa ccgatattgt caacgttcgg acggcggcct acggctgcca
    10201 gcatatattc ggcaacaaat acgccttttt cgccatcctg ctcccaatgg acttctacat
    10261 tgccgtctgc gtcgagtttg gcctcggttt tagcatccaa atgcagtttc aattcttcac
    10321 cgaacacggc tttcgcctcg tctgaaacga cggggtcgga aatgccgccg atgatgccac
    10381 ccaaaccgaa aatttcaact ttcacgccca aacggtgcaa tgcctgaccc agttccaaac
    10441 cgataacacc cggtccgaac acggcaacgc gcttaggcag cgtatcccat gagaaaacgt
    10501 catcgttgat aatcaaacga ttgcccaaag actgccattg cggcaggatg acgggacgcg
    10561 aaccggtagc aatcacgaaa ctttttgccg taatttgggt atggtcgtcg atttggacgg
    10621 tatgctcgtc gataaactta gccgagccca tgatgcgctt gtcggcaggc cactcttcca
    10681 catcagcaac gacaaagccg acaaaacggt cgcgctcgga tttaacacgc cgcattactt
    10741 cttcaccgtt gacgacgatg ctgtctttgt ccaaatgcac gccgaacggg tcggtatgca
    10801 gtgcgtggtg gcgcgcttct gcggcggcaa tcaagagttt ggaaggcata caacccaccc
    10861 gcgcgcaggt cgtgccgaac acattgtttt caatcaggta aacattatcc gaatgtaaac
    10921 gggcattgcg aaacgcaccc ataccggcag taccgccgcc gattacgacg acatccgctt
    10981 gaattttttt cataatcttt gtccttccgt tttcgtctga aacattgccg acacagaaca
    11041 tatcggtttc agacggcatt taaatatttt cccaatcaaa aacatcattc agataaataa
    11101 aaacaaaatt tcgattgagt gcaacaatat ccaaattctc ttataaatat agagttattc
    11161 ggagcggcag tgtagaaccg cccgaataac tctatccggt atatcagaca gtttaatcaa
    11221 accgccttaa gcaaacagct atcagttttt agccaagtaa gcttccaaat cttcgctgcc
    11281 gccgatgtat ttgccgccga tgaagacttg aggggcagtc atcttgccgg taatggcgcg
    11341 aacggaagta acggttgcat ctttgcccaa tacgatttct tcgtaagaca aacctttgtc
    11401 ttgcaaagct tgtttggctt tagcgcagaa ttggcaacct ggtttagtga aaattgccac
    11461 agactcttga gccttccaat cgggagcaac gaattgcagc atagtatctg catcggatac
    11521 tttgaaagga tcgcccggtt cttcaggttc gatgaacatt ttttcaacca cgccgtcgtt
    11581 aaccagcatg gagtaacgcc aagagcgttt accgaagccc aagtcttctt taccgaccag
    11641 catacccata ccttcggtaa attcgccgtt gccgtcagga atcatgtaga tgttgtcgga
    11701 ttcttcttcg gcagaccaag cgttcattac gaacgtatcg tttacagata cgcagcagat
    11761 tgcgtcaacg ccgttttctt tgaacgcgcc gaacaattcg ttgtaacgcg gcaggtgtga
    11821 agaagaacaa gtcggggtaa atgcaccggg cagggagaat acgactactt ttttgccttt
    11881 gaacaaatca tcggtagaca catctttcca agtgtcgccg acacgggtgc ggaatactac
    11941 ggaaggtact ttttgaccgg tacgatcttg caaagccatt ttaagctcct taatagattg
    12001 gtttgagaaa taattgtgaa tcgctgggca ttctacccca tgcaaaatga tttgtataat
    12061 ttatagttca aataatataa attatttaaa actatcaaac acaactccga tttgcctgtt
    12121 tcccacatat aaagccgaat tagaaaaaaa caagctgcaa tgaaaagatt ttccggcaac
    12181 tgcgcccttg cgccgcaaat caggacaaaa taccgtctga aaggctttca gacggtattt
    12241 attcatacgg tctcggctta tttcgccaat tcggcacgca atttgtgggt tacgttcatc
    12301 atcacttgga gttgttccag agtttctttc cagccgcgtg ttttcaggcc gcagtccggg
    12361 ttaacccaca gacgttcaac cggtacaacc tcgatggctt tgcgcaacag gtgctccact
    12421 tcggcttctg tcggtacgcg cgggctgtgg atgtcgtaaa cccccgggcc gatgtcgttc
    12481 gggtatttga attcgccgaa cgcggtcaag agttccatgt cggaacgtga agtctcgatg
    12541 gtgatcacgt ccgcatccat tgcagcaatc gcaggcagga tatcgttgaa ctcggagtag
    12601 cacatatgag tgtggatttg tgtgctgtct tcgcaaccgg cagaggacag gcggaaggat
    12661 tcgcccgccc agttcaggta ggcatcccaa tcggcgcgtt tcagcggcaa gccttcgcgg
    12721 atggcaggtt cgtcaatttg gatgactttg atgcctgctt tttccagatc caatacttcg
    12781 tcgttcagag ccagtgcgat ttgtttgcac acggtagagc gaggaatatc gttgcggaca
    12841 aaagaccatt gcagaatggt tacagggccg gtcaacatac ctttcatcgg gcgtttggtc
    12901 aggctttgtg cgtaagtaga ccaggcgaca gtcatggctt caggacggct tacgtcaccg
    12961 aagataatcg gtggtttaac acaacgtgag ccgtagcttt gtacccagcc gtattgggtg
    13021 aatgcaaaac cgctcaacaa ttcgccgaag tattcgacca tgtcgttacg ctcggcttcg
    13081 ccgtgtacca gtacgtccaa atccagtttt tcttgttctt caaccaccaa ggcgatttct
    13141 tttttcatcg cggcttcgta atcggcggca gacagttcgc cttttttgaa ggctgcgcgt
    13201 gcttggcgga tttcggtagt ttgcgggaaa gaaccgatgt tggtcgtcgg cagcagaggc
    13261 aggttcaacc atgcttgttg cgctttgata cggtcggcaa atggagattt gcgttggtct
    13321 gcgttggcag gcaaatcggc caggcgtttg gcaacgtctg cacggtggat ttcgctgctg
    13381 ttggcgcgtg aatcggcagc agcttggctg gcggccagtt cttcggcaac ggaatcgcga
    13441 ccttcgttca atgcggcttt cagaacgcgc aattcttggg ttttttgcag ggtaaatgcc
    13501 aaccaagagt acaggtcggg tttgttggct ttcagttttt cttcaactga caagtcaaat
    13561 ggagtgtgca gcagagagca agagctggag atccacaaac ggtcgcccag tttggcttgc
    13621 agaggctcaa cagtttccaa aactttgttc aggttggcgc gccaaatatt gcggccgtca
    13681 atcacgccgg cggacaggac tttgtcgtag tcggcgaacg cgtccagttg ctcgggagcg
    13741 cgtaccaagt cgatgtgcag accgtcaaca ggcagggctt tcagcaatgc ggcgtgttcg
    13801 gcaacagaac cgaagtaagt gctcaacagg attttggcgt tgactttgct caaagtagcg
    13861 taaacgtctt tgtaggcttc cacccattct ttaggcaggt caacagccaa agcaggctcg
    13921 tcgatttgaa tccattctgc accggcttca accaaagcgt ttaggatttc aacgtaaaca
    13981 ggcagcagtt tttgcaacag gctcaggcgg ttgaattcga ctgtgccttt ttctttaccc
    14041 acccacagga aagtcaacgg acctacaacg gtcggtttgg ctttcagacc gagggcttgg
    14101 gcttcttgca gttgttgaac atagtgtttg gcattggctt tgaattcggt atcggcgtgg
    14161 aattcaggca ccaagtagtg gtagttggtg tcgaaccatt tggtcatttc gatagcgaat
    14221 tggtctttgt tgccgcgcgc cagttggaag aattgttcca aagacaggtt ttggctgtcg
    14281 aagccgaagc gggcgggaat cgcgccggtg gcgacttgca ggtcgaggat gtggtcgtag
    14341 aaagtgaaat cacctacggc aacgaaatcg gcgttggcag cggcctggtg tttccagttt
    14401 ttctcgcgca agtctttagc aacagccagc aattcttgct cgctgatttc tttgcgccag
    14461 tatttttctt gtgcgaattt caattcgcgg aaggcgccga cacgcgggaa gcctgagaaa
    14521 tgtaatgttg tcatgttgaa ctctccagtt ggattttgta aggaccggtt cgatccgaat
    14581 cggtcggtat tgtaaatcag aatggctcgg atggggcggc gttttgcttt tcagacggcc
    14641 tcgtatgccg tctgaagccg ttttcggctt taagggcgca cgcctaaaat atggcaaatg
    14701 gcgtaagtca gctcgctgcg gttaagcgta tagaagtgga aatctttcac gccttcgcgg
    14761 gacaggactt tgaccatatc gatggcgata cttgccgcca ccagattgcg cgtgccttgg
    14821 tcgtcgtcca aaccttcata catttgcgac agccagcttg ggattttgac gttggttacc
    14881 tgcgccattt tgccgagctg cttgaagttg gtaacgggca ggatgccggg gacgatttcc
    14941 acatcgatgc ccaacatcac gcagcggtcg cggaagcgca ggtagcgttc cacatcgaag
    15001 aagaattggg tgatgacgtg gttcgcgccc gcatcgattt tgcgtttcaa attaatcagg
    15061 tcggcttgtg cggatttcgc ttcgggatgc acttcgggat atgccgctac agaaatgtcg
    15121 aagtcggcga cggagcgtaa tagcttgacc aagtcttcgg cgtaaaacgg ttttttctcg
    15181 taaccgggcg gctcgtcgcc acgcagggcg acaatgcggc ggatgccgct gtcccaataa
    15241 tctttggcaa tttggcgcaa ttcgtcggga gaagcgtcga taccggtcag gtgaggcgcg
    15301 gcttccaagc cggtttcctg tttgatgcgt ttgacgatgc cgtgcgtgcg gtcgcgttcg
    15361 ccggaatttg cgccgtaagt tacggatacg aacttgggat gcagggtttg cagacggtgg
    15421 atggagtccc acagcatcgt ttccatttgt tcgtttttcg gcgggaaaaa ttcaaacgaa
    15481 acgttaatgt cgcctttcaa atcagaaaga ctgttattta acgcattgat ttcttttgca
    15541 taattcatgg ctgcgtacct tttattatct tttatatgat attttcaggc aacataataa
    15601 agttacgcca aacatgagtc aatttcaaaa ttttcagatt tatcatgaaa atttctaata
    15661 tattacaaaa atagttaaat ttccttgtac aagccttcta atttatgata aagtttgtta
    15721 tgttataaca attcaactaa ggcgatatat gaaaccgaat attcatcctg acaattatcg
    15781 aaccgtcctt tttttcgaca gcagcgccaa tgaaggctgg ttaatccgct cctgtgccga
    15841 aacacacgga aaaacgatgg tttggacaga cggcaaagaa tatccgctgt tttcgctgga
    15901 tacttcttcc gcatcccatc ccgtctatac tggcaaacag cgcaacgtca acaccgaagg
    15961 ccgtgccagc aaattcaacc aacgtttcca atccgtcatg tcatcgttta gaaaggacaa
    16021 ataatgcagg ttctgtcttc actcaaaacc gccaaacaac gccaccgcga ctgccaaatc
    16081 gtcagacgca ggggcaaagt ttacgtcatc tgcaaaagca atccgcgttt taaagcacgc
    16141 caacgctgac cccctgccct tcctgcttcg gtt
//
LOCUS       DAWGRZ010000031        15790 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000031 DAWGRZ010000000
VERSION     DAWGRZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15790
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.031"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(30..1073)
                     /gene="galE"
                     /locus_tag="WHT44_000685"
                     /pseudo
     CDS             complement(30..1073)
                     /gene="galE"
                     /locus_tag="WHT44_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223275.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            complement(1066..2068)
                     /gene="dcm"
                     /locus_tag="WHT44_000686"
                     /pseudo
     CDS             complement(1066..2068)
                     /gene="dcm"
                     /locus_tag="WHT44_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(2099..4372)
                     /locus_tag="WHT44_000687"
     CDS             complement(2099..4372)
                     /locus_tag="WHT44_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ2532905.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQPLDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(4436..5086)
                     /locus_tag="WHT44_000688"
     CDS             complement(4436..5086)
                     /locus_tag="WHT44_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ2532906.1"
                     /translation="MISVEHVSKQYQMRGGMRTVLDDINFSLQKGEKVGILGRNGAGK
                     STLIRLISGVEPPTSGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(5083..5880)
                     /locus_tag="WHT44_000689"
     CDS             complement(5083..5880)
                     /locus_tag="WHT44_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ2532907.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLKADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGSDVITYENPWYIVLCNLVLLLFGLAMVSKFSKGVEPQ"
     gene            complement(5880..7043)
                     /gene="ctrB"
                     /locus_tag="WHT44_000690"
     CDS             complement(5880..7043)
                     /gene="ctrB"
                     /locus_tag="WHT44_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HEZ2532908.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDVFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(7058..8233)
                     /gene="ctrA"
                     /locus_tag="WHT44_000691"
     CDS             complement(7058..8233)
                     /gene="ctrA"
                     /locus_tag="WHT44_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ2532909.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            8368..9501
                     /gene="neuC"
                     /locus_tag="WHT44_000692"
     CDS             8368..9501
                     /gene="neuC"
                     /locus_tag="WHT44_000692"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEZ2532910.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTCKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGATVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKYQSDKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            9505..10191
                     /gene="neuA"
                     /locus_tag="WHT44_000693"
     CDS             9505..10191
                     /gene="neuA"
                     /locus_tag="WHT44_000693"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEZ2532911.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            10192..11241
                     /gene="siaC"
                     /locus_tag="WHT44_000694"
     CDS             10192..11241
                     /gene="siaC"
                     /locus_tag="WHT44_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HEZ2532912.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIE"
     gene            11325..12812
                     /locus_tag="WHT44_000695"
     CDS             11325..12812
                     /locus_tag="WHT44_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2,8-polysialyltransferase family protein"
                     /protein_id="HEZ2532913.1"
                     /translation="MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQ
                     LNQVQSLIKIQKLTNNLLVILYTSKNLKMPKLVHQSANKNLFESIYLFELPRSPNNIT
                     PKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYLISIAKKKNITTHLIDEGTGTYAP
                     LLESFSYHPTKLERYLIGNNLNIKGYIDHFDILHVPFPEYAKKIFNAKKYNRFFAHAG
                     GISINNNIANLQKKYQISKNDYIFVSQRYPISDDLYYKSIVEILNSISLQIKGKIFIK
                     LHPKEMGNNYVMSLFLNMVEINPRLVVINEPPFLIEPLIYLTNPKGIIGLASSSLIYT
                     PLLSPSTQCLSIGELIINLIQKYSMVENTEMIQEHLEIIKKFNFINILNDLNGVISNP
                     LFKTEETFETLLKSAEFAYKSKNYFQAIFYWQLASKNNITLLGHKALWYYNALYNVKQ
                     IYKMEYSDIFYIDNISVDFHSKDKLTWEKIKHYYYSADNRIGRDR"
     gene            complement(13308..14261)
                     /locus_tag="WHT44_000696"
     CDS             complement(13308..14261)
                     /locus_tag="WHT44_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532914.1"
                     /translation="MHQYYDAVILSVGNGLLKRFFKQNAQLNIASRPLIITLFPGVVF
                     GDQASILSRMGADIVLYNNKHDFRIAETYKKQYKLSCQNILYGYSIFRHASKGCHGER
                     IYFIDQVKIPFKKEERIYTLKKLIALAEKYPEKEFTILLRVADKDITVHQDKHSYIEL
                     AKQFQLPSNLTIKRKSTAQAFQEMGYCLSYSSTMLFEAECKGIPVGVVADLGFSKSYA
                     NQHFLGSGVLVYFDQIDFTSPKIADPDWLDCYATKKVITTDEFNKLLKQVVPLQHDYQ
                     EYLSAVNSIESTKTIFLRKFKKLIRDPKKFFYDSKWLRKVI"
     gene            14642..15661
                     /gene="galE"
                     /locus_tag="WHT44_000697"
     CDS             14642..15661
                     /gene="galE"
                     /locus_tag="WHT44_000697"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ2532915.1"
                     /translation="MKKILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPPGDTTSPYGASKSMVERI
                     LTDIQKADPRWSMILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAV
                     FGDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRA
                     FEAASGLTIPYEVKPRRAGDLACFYADPSYTKAQIGWQTQRDLTQMMEDSWRWVSNNP
                     NGYDD"
BASE COUNT         4606 a         3620 c         3153 g         4411 t
ORIGIN      
        1 aggatggttt ttttgtttgc ggtttgcatt taatccccat atccgttggg gttgcggctg
       61 acccaacgcc acgaatcctc catcatttgc gcaaggctgc gtttggtttc ccagccagtc
      121 tcttgtttgg tatgggacgg gtcggcaaag gaacacgcca agtctccggc gcgtcggggt
      181 tggatttgat agggaatatt taagccggat gcggcttcaa aggcacggat gatttccaaa
      241 acggaatagg cgcgccccga acccaagttg aacaaatgta cgccggcaac gccgcctttt
      301 gccttcattg ccgcgatatg cccttctgcc aaatccatca catgaatgta gtcgcgcatt
      361 cccgtaccgt cgggggtcgg ataatcgccg ccgaataccg acagttgcgg cagcctgccc
      421 gaagccactt ggcagatata gggcaaaagg ttgttgggaa taccgttggg ctgttcgccg
      481 atcaagccgc tttcgtgcgc cccaatcggg ttgaaatagc gcaacaaaat cacgctccaa
      541 cgcggatcgg ccttttggat gtcggttaac atccgttcca ccatcgcttt ggatgcgcca
      601 taaggattag cggtatcgcc cgggcgcata tcttccgtat agggcatttt acgtatcatg
      661 gcgcgacgca ttaaagtaaa cgccaaagcg cgtttatact ggttatccag tttccctata
      721 tttttacgtt acaaatcaag ttgttggtat tcatcgtgaa aatagaattt ttcggcataa
      781 cgctgactca taaaaccggc atgcgggccg cctgaaaaaa tcattgcgac atcatcgtca
      841 tttaatgttt catggttgtt ttctattaat gctaaagcaa tttggtaatt aattgccaaa
      901 atatcgttag taatcacacg atagccgcgt tttttggctt cgtaggcaaa ggaacagcct
      961 ccactaaata catctgcaac tgtatctaca tcagacggaa gctggtcaca aatccaggaa
     1021 gctattttct ctttattacc gatatagtta attttcggat attgcttatt cattttcact
     1081 catttttaaa atactttcag caatagcttt tgccaataaa ggcggtacgg cgttaccaac
     1141 ctgctgctgc tgggcaattt tgctgccgca aaaaataaaa ttatcaggga aagattgtaa
     1201 ggcagctaat tcacgaacgg ttaacgcccg attctgttca tagtgaaaaa ctttgcgcat
     1261 atctcctgta atacaaacgg ctggttttgt tgctgttgta acggatgtat ttacggatat
     1321 cacctgtttt cggacgtaat ggttcaggaa tatcgttacg gttacctcca tttttaacaa
     1381 atgccatttt ttctaacatt tgtgccgaat gattcatagc ttcatgattt gcaacgtgtg
     1441 gattgctttc gccagcagcc agttttggaa aatgtcctat tgctgatcca acagtctgat
     1501 gggaaatctg caaaggttcg ggaaaggaaa ttttgccttt atccctcctc ccgataaata
     1561 tcactcggct acgtatctga ggaacaccga aatcggctgc actcagtatc ttacattcca
     1621 ccgaataacc gatattctga aatgcttgaa taatctcaat acgtgtttta cctgaattgt
     1681 gtgtatagag tcgcgctaca ttttccataa caaaaaaata tggttggaca attttaacta
     1741 ttcggacaaa ctctttaaat aaatggttgc gtgggtcatc tgtaaatgtc cgtccaatct
     1801 ttcctgccat actaaaacct tgacaaggtg gtcctccaat aatcaaatca actgcttgtc
     1861 cgttaagaca attgattaaa tcttgttcgg ttagtgtggt taaatctttt tgcagtaatt
     1921 gatgatgggg gaagttggta cggtaagtct gacaataatc agactccatt tcaacagaaa
     1981 gcaattgttg gaatccggct tgttcaaaac ccaaggatag gcctcctgct cctgagaaaa
     2041 ggtcaatata ggtaagtggt gtgtgcataa gtcaaaatcc ataaactctt cgattatttt
     2101 accgcttcag cttcgcaaac gcatccgcca tcgccgaatt ggctggggcg cggtcgttgc
     2161 gttggggttt gcggccggct gtccgttcgc ggctgcggtt ttcagacggc attttatgtt
     2221 ttgcgccgcc cggttcgtca tccaagcgca tggtcagcgc gatgcgtttg cgtgcagcat
     2281 cgacttccag cactttcact ttcaccacgt cgccagcttt caccacttcg cgcgggtctt
     2341 ggacgaactt gttggacagg gcggagatgt gcaccaagcc gtcctgatgg acgccgatgt
     2401 ccacgaacgc gccgaagttg gcgacgttgg aaaccacgcc ttcgagtatc ataccgactt
     2461 gcaagtcgct gatttcgtgg ataccttcgg caaacgatgc cgtctgaaac tcgccgcgcg
     2521 gatcacggcc gggtttttcc agttcggaca ggatgtccaa aatggtcggc aggccgaagc
     2581 gttcgtcggt gaagtcggac gctttgattt gcttcacgcg ctcgcggttg ccgatgagtt
     2641 cggcggcgct aatgccttgt tgcgccagca ttttggcgac gacgggatag gcttcggggt
     2701 ggacggcgct cgcgtccaac ggctctttac cgccgttaat ccgcaaaaag cctgccgcct
     2761 gctcgaaggt tttttcgccc aaacgcggta ctttcagcaa ttttttgcgg ctgtcgaacg
     2821 cgccgttttc atcgcggtag gcaacgatgt tttgggcaag ggtttgattc aaaccggaaa
     2881 ttcgcgccaa gagcggggcg gaggcggtgt tcacgtccac gccgacggcg ttcacgcagt
     2941 cttcgaccac cgcgtccagc gatttggcga gctggctttg gttcacgtcg tgctgatact
     3001 gccccacgcc gatggatttg gggtcgattt tgaccaactc ggcaagcggg tcttgtagcc
     3061 tgcgggcgat ggacaccgcg ccgcgcaggg aaacgtccaa gtcggggaac tcgcgcgccg
     3121 ccagttcgga cgcggaataa atcgacgcgc cggcttcgga cacgacgatt ttatgcagcc
     3181 ccattttcga catttcacgc accagttcgc ccgcgatttt gtcggtttca cggctggcgg
     3241 tgccgttacc gatggcgatg agcttcacgc cgtgctgttt aatcaggcgc gacagcgttg
     3301 ccaacatatt gttttcttga tgcaaataga cgatgacggt atccagcagc ttgccggtgt
     3361 cgtccaccac ggcgcatttc acgccgttgc ggtagccggg gtcgagaccc agcgtggtca
     3421 gccgtccggc gggcgcagca agcagcaagt ctttgagatt gcgggcgaac acggtaatcg
     3481 cgtcggtgtc ggcggcttct ttcaaacgat tgagagcttc aagttccaac gacaaaaaga
     3541 ttttcgcgcg ccaagtcagg cgcacggtgt cgcgcagcca tttgtggccg tctgaaactt
     3601 tgaagcggcg ggcgatgatt tgctcgtatt cgctttgctg cgtaatcggc gtgtcgtcgg
     3661 gctgatattt gagcgcgatg ttcaacacgc cttcgttgcg tccgcgcaaa accgctagcg
     3721 cacgatggct gggcatcgca cgaataggtt cgcggtggtc aaaataatcg ctgaattttt
     3781 cgccttcggt ttctttgcct ccaacaactt gcgcatggat ttcggcttcg ttccacaact
     3841 tatcacgcag cgtgccgatg agttccgcgt cttcggcaaa ctgctccatc agaatcgcac
     3901 gcgcaccgtc caacgcggct ttggcatcgg ggacgttttc gttcaggtag ccttgcgcgg
     3961 cagcttccac gtccagcggc tgctcggcaa gcaacacgtc cgccagcggc tgcaaaccgt
     4021 gttcgcgcgc gatttgcgct ttggtgcggc gtttgggttt gtagggcaga tacaggtctt
     4081 ccagcgcggt tttgttatcg gcggcttcga tttgcgccct gaggtcgtct gaaagcttgc
     4141 cttgctcttc aatgcttttt aaaacaacgg ctttgcgctc ttccaactcg cgcagatatt
     4201 gcagccgctc ggcaagccgg cgcaactgcg tatcgtccaa cccgcccgtc gcttccttgc
     4261 ggtagcgggc gataaacggc acggtcgcgc cgtcgtccaa aagctcgacg gcggcggtga
     4321 tttgcgcggc agtcgcggag agttcttggg aaagaatttg agtaatgttc atcaatagaa
     4381 ttccaacgga caggccgtct gaaatttcag acggcctgat ttaaaaacaa tcgctttaag
     4441 gcagcgaatt ataatattcg taggctttgt ccatatcttc aaactggtac atatgccctt
     4501 tttccagcac catcgcatta tcgcaatatt gcttcatggc gctgtggctg tgcgacacca
     4561 agatgatgga acggtctttg cgcttttcaa acaactcgta cttacattta tcggcaaaac
     4621 gcgagtcacc aactgcaatc acttcgtcaa tcaaatagca atcaaactct accgccaacg
     4681 acaaagcaaa tgccaaacgc gccttcatac ctgaagaata acgcttgacc ggctcgtaaa
     4741 gatattgccc caactcggaa aactcttcgg taaagttctt cacataatcg atatcgacat
     4801 tgtaaatccg gcagatgaaa cgcaaattgt ccataccggt cagactgcct tgaaacgcac
     4861 cggagaatgc caaaggccaa gaaatactca tggttcgctt gatttcgccc gaggtaggcg
     4921 gctcgacacc actaatcaaa cggatcaacg tcgatttacc tgcaccgttt cgacccaaaa
     4981 taccaacttt ctcacccttt tgcagcgaaa aattgatatc gtctaacacc gtccgcatac
     5041 cgccgcgcat ttgatactgc ttggaaacat gttcaaccga aatcattgcg gctcgactcc
     5101 tttactgaat ttactgacca tcgccaagcc aaacaacaac aacaccagat tgcacaatac
     5161 gatataccaa ggattttcat aggtaattac atcgctgcca aaatatccgg cacggaacat
     5221 ttctgtgcca tgcaccatcg ggatcatcaa tgcatactct tgcactttcg gaggcaaatt
     5281 atgtacaaaa aagaatgcac ccgataacgg catcatcaca aaagtcaatg tgccccaaat
     5341 cttgccaaac ggctcgaaat taaaggcaat cgaacaaatc accaaaccca aaccaatcgc
     5401 aaaaaaagcc atcaaaagcc aagccatcag catataaaac atatctgccg gcatttcaat
     5461 ccagccaatc gcaatcaata ccgccataat cacaatctgc gcaatggttg caccagcaat
     5521 ttccaaaatc atgcgcgcca agatggtatc caaaactctt acattgcggt gataaagcaa
     5581 gctggcattt gaagaaatcg acccaactgc ccgttttgag gcattacgcc acatcatcaa
     5641 catcggatag ccagtaatcg caaatgcgac aatattcaaa gttgaatatc ggtctgcctt
     5701 taaaaatttc cacatcaaga cgataacgaa tgtcatcagc aacggctcaa caaacagcca
     5761 taaaaagcca atattattgc gaccgtaacg ggtgataatt tcccgcatca aaagcgcacc
     5821 gattacgcgc ctttgaatgg ctaaagattc ccaaaatgat gttttatgca aggctttcat
     5881 cagtttttat gctcacgaat gctggcagtc aacaggctca aaataccata aaccatcaag
     5941 ccgataatca gagtggcaac aatgttgtat aaccgtttag gctcatgtgc caaatccggc
     6001 aggctcggtt gcgagatcac ttccaaataa agctgctgac ggtctgcttc aaccttggca
     6061 ctttccaaag aagtcatggc ggctgccaac tgctgctctg ccaactggtt ttccaaatac
     6121 acacgctgat attcggcagc ctgattagac aacgaagaat gcccaccgcc cgaaatggca
     6181 cgtaactgtt ggtcaatttc tttacgcaag ctctgctcac gcgcctgcaa acccggaatc
     6241 tgcggattct ccggagtgac tgctttcacc tgatccagct gggtttgaat cacaatcaat
     6301 tcatcttgca gcttggaaac caaccccatt tgcacttccg attgcgcttt caaatcaaaa
     6361 acgccattgg caatccggta atccgtcaga ttctgagagg cttcctttac ccgctctgcc
     6421 gccgttttca ctacttcttc cgcatagcgc accgtatcag cacgtgcacg atcgttcaac
     6481 tggttaatca atgcttcacc ttgttttaac aaagcctcat tgattttctt agattccagc
     6541 gcatcaaagg aagttacatt caacgtggaa atacccgaaa ccgtatcaaa attgatcatc
     6601 acctgatttt tatagtattg ataaaaagcc tcttcctcgc cacggaatcc aaaaccatta
     6661 aatcggctaa atacatcgcc cttagtttca taaaattcac gcaccggcaa gattttacgc
     6721 agttcatcca aagacgagcg cgaacgcata tactccccaa ccgtgtaaat atcatcttgc
     6781 gcacgggcaa aacctgtgcc ctgcaaaatg gcacccaggc cattgagaga agattggctt
     6841 ttaggcgagc gcaccacaaa gctcgattgc gacgtaaaac gatcggaagc gaagaagccg
     6901 aaatacacca acgaaattac cgtagggata atcaccgtta cccaaaataa agggcttagc
     6961 tttttaatcc aacttttctt tttcggcttt ttacgctcgg ctttggtttc agtggcaact
     7021 gccacaggaa gttgctcaga catcttaatt actcacatta attagttaaa ttattaatac
     7081 tgttcgcgcc actggtaacc ggcgagaaca caaacgacaa gaatttctgc acttcagcca
     7141 acggcgcatt cgacacatac aatacatctt tattcttcac aggaaagcgc tgcatagaaa
     7201 atagcgaatg cgcatcagcc atattcacac gatataccgt tggaatctct gcctcactgc
     7261 cataaccttg agcaatccat ttatcctgac gttctgccgg caattccacc aatggcgtat
     7321 agcggaacac aaacacacca cgcgcatcag aacggcgatc ttgcaaaccg cccatacggc
     7381 caatggcttc agaaagcgat aagcctctgg ctgaaaaacc gatttcttgt gttctcccca
     7441 ccgcacccat agacgtaaag gtatagggat tggtaatcat ggtaaccaca tcaccgcgac
     7501 gcagcaaaat attttgtcgc ggatttgcaa ctaaatcttc caaggcaaca gttcgtacta
     7561 cattgccacg tgtcagctgc acattcgtat cctgcacatt tgccgttgaa ccacctaccg
     7621 cagccaccgc atccaacaca cgctcaccgg ctgccgtcag cggcatacgc acactattcc
     7681 cagcacgaat caccgacaca ttcgccgcat tattctgcac caaacgcacc atcacttgtg
     7741 gctgattggc catttttttc aggcggcctt taataatttc ctgaacctga ccaggcgttt
     7801 taccgaccac cgaaatatcg ccaacaaacg gcacagaaac cgtaccacgt gccgtgacca
     7861 actgctctgg caacttagtt tgatgcgcac tacccgagcc catcgaagaa aggccaccac
     7921 caaacaatac tgccggcggc gcttcccaaa tcataatatc caatacatca ccaatattta
     7981 gcgtaccagc cgaagcataa ccatcgccaa actgagtgaa tgactgattt atctgagcct
     8041 tatataataa ctgagcaacc gtatgattca catcaatcag ctccacttca ggaatttgaa
     8101 cttcagattg ttgccctaaa gagacaattt tttttgcgct ggggcctgat gaaggaatcg
     8161 cagagcatcc tacaattaaa cttccacaca ataataatac tgcgtgacga atataaaatt
     8221 tcactttaaa cacaagccaa atcctaatat aattataaat ggcctaatta tagcacttaa
     8281 tcgaaataaa tttatgagta cgtagagtat aattagtatt cttctttcca acttccttat
     8341 acttatatac ttatagattc taaaatcatg aaaagaattc tttgcattac aggtaccaga
     8401 gccgacttcg gcaagctaaa acctttatta gcctatattg aaaatcaccc agaccttgaa
     8461 ttgcatttga ttgtaactgg tatgcatatg atgaaaacat atggcagaac ctgcaaggaa
     8521 gtaactcgag aaaactatca acatacatat ctgttttcaa atcaaatcca aggtgaacca
     8581 atgggtgccg ttttaggcaa taccattacg tttatctctc gtctatctga tgaaattgaa
     8641 cctgatatgg tcatgattca cggcgaccgt ttagaagcac tagcaggcgc aactgtaggt
     8701 gcattaagca gccgtttagt ttgccatatc gaaggtggtg aactatctgg tacagtagat
     8761 gactccattc gtcattctat tagtaaactt tctcatatcc acttggtagc aaatgaacaa
     8821 gctgtcactc gcctagtgca aatgggagaa aaaagaaagc atattcacat catcggctcc
     8881 cccgatttag atgttatggc ctcttccacc ctcccatcct tagaagaagt caaagaatat
     8941 tacggtttac catacgaaaa ttatggtatt tctatgtttc accccgtgac tacagaagca
     9001 catttaatgc cacaatatgc ggcccaatat ttcaaagcat tagaattaag tggccaaaat
     9061 atcattagca tctaccctaa taatgacact ggcactgaaa gtattctgca agaattatta
     9121 aaatatcaat ctgataaatt tattgcattt ccttccattc gatttgaata ttttttagtc
     9181 ttattgaaac atgctaaatt tatggtcggc aactcaagtg caggtattag agaagctcct
     9241 ctctacggtg tcccttcaat tgatgttggt acacgccaaa gtaaccgcca tatgggaaaa
     9301 tctattattc atacagatta tgaaactaaa aatatctttg atgcgattca acaagcatgc
     9361 agtttaggca aatttgaagc agatgatacc tttaatggcg gagatactcg caccagcaca
     9421 gaaagatttg ctgaagtaat caacaatcct gaaacgtgga atgtttctgc tcaaaaacgt
     9481 tttatcgatt tgaatcttta aattatggaa aaacaaaata ttgcggttat acttgcgcgc
     9541 caaaactcca aaggattgcc attaaaaaat ctccggaaaa tgaatggcat atcattactt
     9601 ggtcatacaa ttaatgctgc tatatcatca aagtgttttg accgcataat tgtttcgact
     9661 gatggcgggt taattgcaga agaagctaaa aatttcggtg tcgaagtcgt cctacgccct
     9721 gcagagctgg cctccgatac agccagctct atttcaggtg taatacatgc tttagaaaca
     9781 attggcagta attccggcac agtaacccta ttacaaccaa ccagtccatt acgcacaggg
     9841 gctcatattc gtgaagcttt ttctctattt gatgagaaaa taaaaggatc cgttgtctct
     9901 gcatgcccaa tggagcatca tccactaaaa accctgcttc aaatcaataa tggcgaatat
     9961 gcccccatgc gccatctaag cgatttggag cagcctcgcc aacaattacc tcaggcattt
    10021 aggcctaatg gtgcaattta cattaatgat actgcttcac taattgcaaa taattgtttt
    10081 tttatcgccc caaccaaact ttatattatg tctcatcaag actctatcga tattgatact
    10141 gagcttgatt tacaacaggc agaaaacatt cttaatcaca aggaaagcta aatgcaaaac
    10201 aacaacgaat ttaaaattgg taatcgttca gtaggttaca accacgaacc attgattatc
    10261 tgtgaaatcg gcatcaatca tgaaggctct ttaaaaacag cttttgaaat ggttgatgct
    10321 gcctataatg caggcgctga agttgttaaa catcaaacac acatcgttga agacgaaatg
    10381 tctgatgagg ccaaacaagt cattccaggc aatgcagatg tctctattta tgaaattatg
    10441 gaacgttgcg ccctgaatga agaagatgag attaaattaa aagaatacgt agagagtaag
    10501 ggtatgattt ttatcagtac tcctttctct cgtgcagctg ctttacgatt acaacgtatg
    10561 gatattccag catataaaat cggctctggc gaatgtaata actacccatt aattaaactg
    10621 gtggcctctt ttggtaagcc tattattctc tctaccggca tgaattctat tgaaagcatc
    10681 aaaaagtcgg tagaaattat tcgagaagca ggggtacctt atgctttgct tcactgtacc
    10741 aacatctacc caacccctta cgaagatgtt cgattgggtg gtatgaacga tttatctgaa
    10801 gcctttccag acgcaatcat tggcctgtct gaccatacct tagataacta tgcttgctta
    10861 ggagcagtag ctttaggcgg ttcgatttta gagcgtcact ttactgaccg catggatcgc
    10921 ccaggtccgg atattgtatg ctctatgaat ccggatactt ttaaagagct caagcaaggc
    10981 gctcatgctt taaaattggc acgcggcggc aaaaaagaca cgattatcgc gggagaaaag
    11041 ccaactaaag atttcgcctt tgcatctgtc gtagcagata aagacattaa aaaaggagaa
    11101 ctgttgtccg gagataacct atgggttaaa cgcccaggca atggagactt cagcgtcaac
    11161 gaatatgaaa cattatttgg taaggtcgct gcttgcaata ttcgcaaagg tgctcaaatc
    11221 aaaaaaactg atattgaata atgcttatta acttagttac tttattaaca gaggattggc
    11281 tattacatat agctaattct cattaatttt taagagatac aataatgcta aagaaaataa
    11341 aaaaagctct ttttcagcct aaaaagtttt ttcaagattc aatgtggttg acaacatctc
    11401 cattttatct taccccccca cgtaacaatt tatttgtcat atctaattta ggtcagctta
    11461 accaagtcca aagcctaatt aaaatacaaa aattaaccaa taatttacta gtaattttat
    11521 atacttctaa aaacttaaaa atgcctaagt tagttcatca atcagctaac aagaatctat
    11581 ttgaatctat ttatctattt gagcttccta gaagccctaa taatataact cctaaaaaat
    11641 tactttatat ttatagaagt tacaaaaaaa tccttaatat tatacagcct gctcatctct
    11701 atatgctgtc ttttacaggc cactactcct atctgattag tattgcaaaa aaaaagaata
    11761 ttacgactca tttaattgat gaagggactg gaacatatgc tcctttatta gaatcatttt
    11821 catatcatcc aacaaaatta gaacgttatt tgattggaaa taatcttaat attaaaggat
    11881 atatagatca ttttgacata ttgcatgtcc cctttcctga atatgctaaa aaaatattta
    11941 atgcaaaaaa atataaccgg ttttttgcgc atgctggagg aataagcatt aataataaca
    12001 ttgcaaactt acagaaaaaa tatcaaatat ctaaaaatga ctatattttt gttagtcaac
    12061 gctaccccat ttcagatgat ttgtattata agagtatagt agaaatctta aacagcataa
    12121 gtttacaaat taaaggaaag atatttatta aactacatcc aaaagagatg ggcaacaact
    12181 atgtaatgtc tttatttcta aatatggtag aaataaaccc tcggctggta gttattaatg
    12241 aacctccttt tctaattgag cccctaatat acttaacaaa tcctaaagga attataggcc
    12301 tggcctctag ttctttaatt tatacaccat tactctcacc ctcaacccaa tgtctttcta
    12361 ttggagagtt aattattaac ttaattcaaa aatattcaat ggtggaaaac actgaaatga
    12421 tccaagaaca cttagagatt attaagaaat ttaattttat taatatacta aatgatttaa
    12481 atggggtaat aagtaaccct ctctttaaaa cagaagaaac atttgaaaca cttcttaaat
    12541 ctgcagaatt cgcatataaa tctaaaaact actttcaggc tattttttac tggcaacttg
    12601 ccagcaaaaa caatattacc ttattagggc ataaagcatt atggtactac aatgcacttt
    12661 ataatgtaaa acaaatttat aagatggaat attcagatat tttttatatc gataatatct
    12721 ccgtagactt tcatagtaaa gataaattga catgggaaaa aattaaacat tattactatt
    12781 ccgccgacaa tagaattggt agagatagat aaaaatacta cgacttctga tctccatacg
    12841 tatgcttgtt ttcaaaaagc agcattaaga tagataagtg aagcttgatg agagccattt
    12901 ttgtggtcat cgacaaggta cacgcggaga gtaatcgaca acagagctgc tgtattctgg
    12961 cttttaaagc ccagtagtaa agtttataag gctgcatttc tcaatgtaaa acgcgatatt
    13021 ttcacccatt attagggata agtagccagt ggtttacact ggctactttc atagttatga
    13081 agtactgggt ctgagtgaat tcaattattt ccgtataaaa cacagtagac attttactaa
    13141 tcggcaaaat tatattagaa gaattgagaa ctactgaagt caggtaagat accattttgc
    13201 acaaatttaa cggcatccct aaaaagcatt ttgggtggta tttaaaggag tacaaaatgg
    13261 ttttaactac agtatgctta gaaatctata gccttacgcg ctttatatta aatcaccttt
    13321 ctcaaccatt tagaatcata aaaaaacttt ttaggatccc taattaactt tttaaatttt
    13381 cttaaaaaaa tagttttagt tgattctata ctattcaccg cagataaata ttcttggtaa
    13441 tcatgttgca atggcacaac ctgctttaat agcttattaa actcatcagt tgtaatcacc
    13501 tttttagtag catagcaatc aagccaatcc ggatctgcta tttttgggga agtgaaatct
    13561 atttgatcaa aataaactaa aaccccacta cctaaaaaat gctgatttgc ataggattta
    13621 gaaaagccta agtctgcaac aacaccaaca gggataccct tacattcagc ttcaaaaagc
    13681 atagtagatg aataagataa acaatacccc atttcttgga aggcttgcgc ggtacttttt
    13741 cgctttattg tcaaattact cggcaactga aactgctttg ccagctctat atacgaatgt
    13801 ttatcctgat gcacagtaat atctttatct gcaaccctta gcaaaatagt aaattctttc
    13861 tcagggtatt tttcagccaa ggcaatcaac ttttttaatg tgtaaattct ttcttctttt
    13921 ttaaatggaa ttttgacttg gtcaataaaa taaattctct ctccatgaca acctttcgaa
    13981 gcatggcgaa aaattgaata accataaagt atattttgac aacttaattt atattgtttt
    14041 ttatatgtct ctgcaattct aaaatcatgc ttattattat ataaaacaat atcagccccc
    14101 atacgagata gaatacttgc ctgatcaccg aatactacac ctggaaacaa ggtaataatc
    14161 aatggccttg aagcaatatt taattgcgca ttctgcttaa agaacctttt taacaaccca
    14221 tttccaaccg ataaaattac tgcgtcataa tattgatgca tattcttaat aaagtaatca
    14281 tcaatatgaa aaaaaattgc ttggtcttta tctatccctg attctaataa ctgtcgattt
    14341 gaaagaatgt tttctcgact tttgtggata taaatatcaa tttgagcatc ttttatctct
    14401 tttgcaacag catagcccga gttaaggaac gagtcataac tggcaattag taatactttt
    14461 ttcactattt tcccttaatt ctacaccata tgaataaaat tatagtattt taccttagat
    14521 atcaaatata cttaaccaaa aaaatctgaa gaaatctact gctacttcag ggataattat
    14581 ttcattaaga ttttacaacc attattatca atctatcaaa gtatttttag tattttattc
    14641 tatgaaaaaa attctcgtta ccggcggcac cggttttatc ggctcgcata ccgttgtttc
    14701 tttgctgaaa agcggccatc aagtcgtgat tttggataac ctatgcaatt ccagcatcaa
    14761 tatcctgcca cgcttgaaaa cgattaccgg ccaagagatt ccgttttatc aaggcgatat
    14821 ccgcgatcgt gagattttgc gccgtatttt tgcggaaaac cgcattgatt cggtgattca
    14881 ttttgccggc ttgaaagcgg tgggtgaaag tgtggccgag ccgatgaaat attatgataa
    14941 taatgtttcc ggcagcttgg tgttggcgga agaaatggcg cgtgcgggcg tgtttagcat
    15001 tgtgttcagt tcttcggcga cggtttatgg cgatccgggc aaagtgcctt ataccgagga
    15061 tatgccaccg ggcgacacca ccagccctta cggcgcatcg aaatcgatgg ttgagcgcat
    15121 tctcactgac attcaaaaag ccgatccgcg ctggagcatg attttgttgc gttatttcaa
    15181 tccgattggc gcgcatgaaa gcggcttgat tggcgagcag ccaaacggca tcccgaataa
    15241 tttgttgcct tatatctgcc aagtggcggc aggcaaactg ccgcaattgg cggtatttgg
    15301 cgatgactac cctacccccg acggcacggg gatgcgtgac tatattcatg tgatggattt
    15361 ggcagaaggc catgtcgcgg ctatgcaggc aaaaagtaat gtagcaggca cgcatttgct
    15421 gaacttaggc tccggccgcg cttcttcggt gttggaaatc atccgcgcat ttgaagcagc
    15481 ttcgggtttg acgattccgt atgaagtcaa accgcgccgt gccggtgatt tggcgtgctt
    15541 ctatgccgac ccttcctata caaaggcgca aatcggctgg caaacccagc gtgatttaac
    15601 ccaaatgatg gaagactcat ggcgctgggt gagtaataat ccgaatggct acgacgatta
    15661 agttgacctg atacaggccg tctgaaagag atgttttcag acggcctctt tatctgaaaa
    15721 acacacattc tgtctgctat aatctgttta tattttttgg ctatcctctg aaatttatga
    15781 gaaaaatcct
//
LOCUS       DAWGRZ010000032        15406 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000032 DAWGRZ010000000
VERSION     DAWGRZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.032"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(136..1920)
                     /gene="lpdA"
                     /locus_tag="WHT44_000698"
     CDS             complement(136..1920)
                     /gene="lpdA"
                     /locus_tag="WHT44_000698"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ2532916.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1938..2375)
                     /locus_tag="WHT44_000699"
     CDS             complement(1938..2375)
                     /locus_tag="WHT44_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532917.1"
                     /translation="MGNFLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKA
                     THGPSVKNAVYAHQIETGLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQ
                     YSIFEYEVEHPENPNEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(2469..4076)
                     /gene="aceF"
                     /locus_tag="WHT44_000700"
     CDS             complement(2469..4076)
                     /gene="aceF"
                     /locus_tag="WHT44_000700"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ2532918.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPAA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGSAIIEVETVGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAPS
                     APAAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIMGDDIKAFVKSVMQ
                     GGAAKPAAASASLGGGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPH
                     VTVHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDN
                     LVLKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQG
                     ACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDH
                     RVIDGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(4226..6889)
                     /gene="aceE"
                     /locus_tag="WHT44_000701"
     CDS             complement(4226..6889)
                     /gene="aceE"
                     /locus_tag="WHT44_000701"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ2532919.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7583..9295
                     /locus_tag="WHT44_000702"
     CDS             7583..9295
                     /locus_tag="WHT44_000702"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ2532920.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYQDMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAELVKVHTPNVKTIDSLVDFLSIPIEK
                     TLKSIVVEGENEGELILLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFAGKVYADFATEKGADWVIGANEDDYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRDNEAQAVAIGEIVARVTA
                     SLNA"
     gene            9358..10014
                     /locus_tag="WHT44_000703"
     CDS             9358..10014
                     /locus_tag="WHT44_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ2532921.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPEKVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(10024..10479)
                     /gene="ruvX"
                     /locus_tag="WHT44_000704"
     CDS             complement(10024..10479)
                     /gene="ruvX"
                     /locus_tag="WHT44_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ2532922.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(10472..11020)
                     /locus_tag="WHT44_000705"
     CDS             complement(10472..11020)
                     /locus_tag="WHT44_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ2532923.1"
                     /translation="MNLSNHFLIAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWSKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(11022..11582)
                     /locus_tag="WHT44_000706"
     CDS             complement(11022..11582)
                     /locus_tag="WHT44_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ2532924.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENLDKR"
     gene            11910..13700
                     /locus_tag="WHT44_000707"
     CDS             11910..13700
                     /locus_tag="WHT44_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ2532925.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAATKAQI
                     SRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQKALAV
                     QEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLEQKGNE
                     QQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRAEMSNL
                     TAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDIWKGVFYSTA
                     PATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAAGSKIG
                     SSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13827..15311
                     /locus_tag="WHT44_000708"
     CDS             13827..15311
                     /locus_tag="WHT44_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ2532926.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAIPEDYVYGMGGDSLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNGETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT         3697 a         3795 c         4252 g         3662 t
ORIGIN      
        1 ccttgtcctg atttttgtta atccactata ggtcggagaa gatattttaa tccgctgtaa
       61 aatagaaaat gccgtctgaa catttcagac ggcatttgct caaataaacc ttatcggctg
      121 tttattcagt cggatttatt ttttcttttg cggaggcagg tcggtacaag tacccaatgc
      181 cacttccgcc gccataccga tggattcgcc caaggtcggg tgcgggtgga tggttttgcc
      241 gatgtctgcc gcgtcgcagc ccatttcgat ggcaaggcag acttcgccga tcatatcgcc
      301 accgttcgga ccgacaatgc cgccgccgat gatgcggccg gtttcggcat caaaaatcag
      361 cttggtaaag ccgttgtcgc aaccgttggc aatcgcacgg ccggaagccg cccacgggaa
      421 gttggctttg gtgattttgc ggccggaggc tttggcggac agttcggttt cgcccaccca
      481 cgccacttcg ggggaagtgt aggcaacgcc cggaatcacg cgcgcgtcga agtaggcttt
      541 gtggccggcg cagttttcgg cggcaacgtg gccttcgtga acggctttgt gcgccaacat
      601 cggctgaccg acgatgtcgc cgatggcgta gatgtgcggc acattggtac gcatttgttt
      661 gtccacttcg atgaagccgc gatcggttac ggcaacgcct gctttttccg cgctgatgag
      721 tttgccgttg ggcgcgcggc cggcggcaac caatacggca tcgtagcgtt gcggctcttt
      781 aggcgcgttc gcgccttcaa aggtaacgta aacgccgtct tctttcggct caactgcaac
      841 ggttttggtg ttgaccataa tgttgtcaaa acggtattcg ttttgttttt gccatacttt
      901 taccaaatcg cggtctgcgc cttgcatcag gccgtccatc atttcaacca catccaaacg
      961 cgaacccagc gtgctgtaaa ccgtacccat ctcgaggccg ataatgccgc cgccgataat
     1021 cagcagtttg cccggtactt ctttcagagc caatgcgccg ctggaatcga tgatgcgcgg
     1081 atcttcagga atgaaaggca gtttggttac gcggctgcct gctgcaatga tacagttttt
     1141 gaaggcaacg atttttttct cgccggtagg ggctgcctgt tcgtacgcgt cgccggcagt
     1201 cagcgacact tccaagtggt gcggatctaa gaattgcccg tcgccttgga taacgtccac
     1261 tttacggctt ttcgccatac ctgccaaacc gcccgtgagg cgggaaacta cgccgtcttt
     1321 gtaggcgcga agcatatcga tgtcgagttc cggctcgggg tatttgatac cgttggcagc
     1381 caagtggcgc acttcgtcga taacggcggc attgtgcaac aaggctttgg aagggataca
     1441 gccgacgttc aggcaaacgc cgcccaaagt tttgtaacgt tcgacgatgg cgactttcaa
     1501 gccttcatcg gcagcggcaa atgcagcgga gtaaccgccg ggaccgccac ccaatacgac
     1561 cacgtcgtac tcggcatcgg cagaaccgcc gaattgcgcg gcttgcggag caggagcggc
     1621 agctttaggg gcttcttgcg ccggggcggc agccgcttcg gctttaggag cggctgccgt
     1681 gccttcagct tcaacgacga caatcaaacc accttcagag attttgtcgc cgactttaac
     1741 tttaacttct ttgactacgc ctgcaacttc agcaggtacg tccatagtcg ctttatcggt
     1801 ttccaaagta atcagggtat cgtccacagc aatagtgtcg cccacgttta cttcaaccgc
     1861 gataatatct acattttcgt gtccgccaat gtcgggcact ttcaattcaa ctaaagccat
     1921 tatattgacc tttcaactta gttaatttct atcaactctt tagcaataat cacttcttca
     1981 ggaatacagc cacaatcttc agctctgatt gttacttcct tctcatttgg gttttctgga
     2041 tgttcaacct catattcaaa aatagaatat tgaccaaaca aatccctatt taaaacatat
     2101 atatagccat tttcgatgcc ggaacttgtt gcaaatttct tggcaatttc cttgtctgtt
     2161 gtcgtagata tataacatcc gtcatataga cctgtttcaa tttgatgggc gtaaactgca
     2221 ttcttcacac ttggaccatg tgtagcttta ccatcatatt taaacttacc atcataacga
     2281 attgcaactt cagctttatt acctttaggt tttaactgtc cattattttg ctcatcttgt
     2341 tggcaactaa tgcctctata taagaaattt cccataagtt attaatttaa aggttgaatt
     2401 taacaccatt actcatcaat ctcaagcaat ccaaacatta tcagactgct tgagagggat
     2461 gttttatttt ataaggtaat gcggcggaag tctttcaaca gcttcgccaa gaatacggtg
     2521 aagcgcatac cggccgcacc gtcgatgaca cggtggtcga aggacaggct caacgggcac
     2581 atcaggcgcg gggcaaactc tttgccgttc caaacaggtt tgatttggga tttgcacacg
     2641 cccaagatgg cgacttcggg agcgttcaca attggcgtga agcctgtgcc gccgatgccg
     2701 cctaagctgg aaatggtaaa gcacgcgcct tgcatttctt gcggtttgag cttgccttca
     2761 cgggcttttt tggacaattc ggtcaattct tggctgattt gtttcaagcc tttttgatcc
     2821 acgtctttga tgacgggaac aaccaagccg ttcggcgtat cggctgcgaa accgatgttg
     2881 aagtagtttt tcagcaccag gttgtcgccg tccagtgagg cgttgaattc ggggaatgct
     2941 ttcaacgcgg aaacagaggc tttgatgatg aacgccaacg gggacagttt cacgccttcg
     3001 cgttcccatt ctttgttcag ctgtttgcgg aattcttcca gctcggtcat gtccgcttct
     3061 tcgtgtacgg taacgtgggg aatcacaacc cagttgcggg acaggttttg accggaaatt
     3121 ttcttaatgc gggacaattc tttaacttcg acattgccga atttggagaa gtccacttta
     3181 ggccacggca gtaagtccag accgccgccc aaagatgcgc tggctgcggc aggttttgcc
     3241 gcgccgccct gcatcacgga tttcacaaag gctttgatgt cgtcgcccat gatacggcct
     3301 ttcaagccgg tgcctttgac ttggcccaaa tccacgccca attcgcgcgc cagtttgcgt
     3361 gcggaaggac cggcgtgtgc tttggcgaaa gcggcctcgt cgattttggc agcggcaggt
     3421 gcagaaggtg caggcgcggc ggcgggtgcg gcggcagcag tcggaggcgg agcggcagcc
     3481 ggtgcggcag cttgagcggc ttgagcaggg gctgctgcgg cagagccgac ggtttctact
     3541 tcgataatgg cagagccttc ggatactttg tcgccgactt ttaagaatac ggctttaacg
     3601 acaccggcag cggtacaagg tacgtccatt gtcgctttat cggtttccaa agtaatcagc
     3661 gtgtcgtctt cggcaacggt gtcgcccact ttgatttcaa cggcgattac atccacatcg
     3721 gtatggccgc cgatatcggg aacggctact tgaacggttg caccgcctgc ggcagcgggt
     3781 gcggcagcag gtgcaggttg tgcttcggca gcagccgggg cggcttcggc ggcggcggca
     3841 ccggtttcaa cggtcagaat tacgccgcct tcggagattt tgtcgccgac tttgactttt
     3901 acttctttca cgacaccggc cgcatcggca ggcacatcca tcgtggcttt gtcggtttcc
     3961 agtgtaatca gggtgtcgtc aacggcgatg gtgtcgcccg ctttaacttc tacggcgatg
     4021 atgtcgacgt tttcgtgacc gccgatatcg gggactttga tttctacgat actcatttga
     4081 gttcctttaa tctttttcaa ttggatgccg tctgaaagct gcttcaggcg gcatcggtac
     4141 ggtttgtctg ctcaaaccat tcgggcattc ggttttcaga cggcctgatg tcggaatggg
     4201 gcaaacaggc cgtctgaaac atcaatcagc gtttccagct aggagctgaa tcggctttga
     4261 tgccgtattt ctcaatggct tgttgaacgg tttctttgct gactttgcct tgttccgcca
     4321 atgcggccaa tgcggccacg gcaacgttgt agcgatccac ttcaaagaag cggcgcaggt
     4381 tggcgcgact gtcggaacgg ccgaaaccgt cagtgcccaa gacgtggtag tcgttcggga
     4441 tgtaggcgcg gatacggtca gcatagctgc ggatatagtc ggtagcggca ataaccggac
     4501 cgtcatgacc ttgcagttgg gaagtaacga aaggtacttt ttcggcttcc agcggatgca
     4561 ggcggttgaa gcgttctacc tcgacagcgt cgcggtgcag caggttgaag gacgggcaag
     4621 accagatgtc tgcttctacg ccgaagtcgg ctttcagcag ctcggcaccg gcaatgactt
     4681 cttgcaggat ggtaccggag cccatcaatt gaactttctt atcgcctttg ccgccggctt
     4741 tcagcaggta catacctttc aagatgtctt gttccgcacc ttcgggcata tccggatggg
     4801 tgtagttctc gttcatcagg gtgatgtagt agaacacgtc ttcattattg gcatacatac
     4861 ggcgcagacc gtcttgtacg atgacggcga cttcgtattg gaaagtcggg tcataagata
     4921 cgcagttcgg aatcaggtcg gcctggatgt ggctgtggcc gtcttcgtgt tgcaggcctt
     4981 cgccgttcag cgtcgtacgg ccggcagtac cgcccagcag gaagccgcgc gcgtgcatat
     5041 cgcccgccgc ccaagccaag tcgccgatac gttggaaacc gaacatagaa tagtaaatgt
     5101 agaacggaat catggcgaag ttgctgttgg cgtagctggt tgcagccgca atccagtcgg
     5161 ccatcgcgcc cggttcgtta ataccttctt gcaagatttg accgtcaacg gattctttat
     5221 agaacatcag ttggtctttg tcttgagggg tatattgctg acctttcgga ttccaaatac
     5281 cgtattggcg gaacatacct tccatgccga aagtacggct ttcgtcggga acgataggta
     5341 cgacgcgttt gccgattttt ttgtctttca gtaaagtgga caggatgcgg acgaatgcca
     5401 tcgtggtcga gaactcgcgt tcaccgctgg atttgagttg tgcgtcgaat gctgacagct
     5461 cgggcacttc caatacttcc tgcgtcggtt tgcgttgcgg caggtagccg cccaaagcat
     5521 cgcggcgtgc gtgcaggtat ttgtattctt ccgtatcggg ggcaaaagtc aggtaaggca
     5581 gatcgccgct ttcgatttgt tcgtcggtaa ccggaatgtc aaagcggtcg cggaattgtt
     5641 tcagggacgc tttgtccatt tttttggctt ggtgggcaac gttctgacct tcgccggatg
     5701 cgcccatacc gtaaccttta atggttttcg ccaagatgac ggtaggtttg ccgtcggcat
     5761 ggttcgctgc gcggtcgtag gcgttgtaca ctttttgcgg gtcgtggccg ccgcggttca
     5821 atgcccagag ttgctcatcg gtcatatcgg caaccaatgc tttcagttcg ggcgtattga
     5881 agaagtgttc gcgcacatac gcgccgtctt tggatttgta agtttggtag tcgccgtcca
     5941 aacattcttc catacgttgg cgcaggatac cgtctttgtc tttcgccaag aggcggtccc
     6001 agcggcggcc ccaaatgact ttgacgacat tccagccggc gccggcaaag ttgccttcca
     6061 attcttggat gattttgccg ttgccgcgta ccggaccgtc caagcgttgc agattgcagt
     6121 tgatgacgaa aatcaggttg tccaagcctt cgcgtgcagc cagtgcgatt gcaccttgag
     6181 attcgggttc gtccatttcg ccgtcgccgc agaaacacca tactttacgg cctttggttt
     6241 ttgccaaacc acgcgattcc aagtatttca ggaaacgcgc ctgataaatc gccatgatgg
     6301 gccccaagcc catggatacg gtcgggaact gccaaaagtc gggcaagagg tgggggtgcg
     6361 gataggaagg cagaccgtgt ccgtccactt cttggcggaa gttgttcagc tggtcttcgg
     6421 tcagacggcc ctcgacgaat gcgcgtgcat agatgcccgg ggcgacgtga ccttggaaga
     6481 agaccaaatc gccttcttcg ccttcgcctt tggctttcca aaagtggttg aaaccgactt
     6541 cgtacatggt ggcggcagat tggaaagatg cgatgtgccc acccagttcc aaatctttct
     6601 tgccggcgcg caatacgatg gcggcggcgt tccagcgcac gaatgcgcgg atgcggtgtt
     6661 cgatgttttg gtcgcccgga atgccttttt cgttttcaac cgaaacggta ttcaaatacg
     6721 gggtggtcgt gccgtgtggc atacgtacgc ccttgtcgcg gcagtatttg accaggtttt
     6781 ccaagaggta ttgcgcgcgt tcgccgcctt catattcgag gacggagctt aacgcgtcca
     6841 gccactcttg ggtttcgata gggtcaacat cgtgtaattg ggtggacata gtatctatcc
     6901 tttatgttga gtgtttaatc ggcagggagg gtgtagccaa cccctttatt ttcgtttgtg
     6961 aaaaataata agcaaaaacg aaaatttcta aatcaaaccg aaaaaaataa tttaagttta
     7021 atgttttcaa aaaattataa aagttttgga aacaagggaa aacttgaaaa aaatgttcga
     7081 tctgctccac acgaaatcct atcagcaatc gggtctgctg gcaaataacc atgcctgtaa
     7141 aagcaggttt tatactgaag ttggcgtttg gtaaggtcaa gtctttttga tttcgaaata
     7201 ttctgtttga ttttggcgat attttttcgg aattgtggta tatcgaatca ttatgtctct
     7261 gtcggcaatt ttcctattgt agaaaatcta tacacaagct ttgtggataa ccgtgttgat
     7321 aagccacggc ttgtgttaaa aaagtcttac gcaacaaatg tacgcttatt ttgattaaaa
     7381 aataggcaca tttaaatcta taaaaatcat gtgcttatgt tcaatatgat gtttctggtc
     7441 atgtttttgc cgtctgttat gggatttgtc ccgcgcttgg ggcagggggt gaattttgga
     7501 ggttggtgca aaacctgctt ttttcgcgtt acaatctgca cccgatttac ttttgaattt
     7561 gcctgtttgg aaaaatactt ttatgaaagc cagccaattc tttatctcta ctttaaaaga
     7621 agcccctgcc gaagccgcgc ttgccagcca caagctgatg attcgcgccg gcctgattaa
     7681 ggccaacgca tcgggtcttt atacttggat gccgatgggg ctgcgcgtgt tacgcaaagt
     7741 cgaaaacgtc gtgcgcgagg aaatggcgcg cgcgggcagc gtggagttgc tgatgccggt
     7801 ggtgcagcct gccgagctgt ggcaggaatc cggccgctgg gagttttacg gtaaagaact
     7861 gctgcgcctg aaagaccgcc acgaccgcga tttctgcatg ggcccgacct gcgaggaagt
     7921 catcgccgac atcgtgcgca aagaaatcaa cagctacaaa cagctgccga aaaactttta
     7981 ccacatccaa accaaattcc gcgacgaagt gcgcccgcgc ttcggcgtga tgcgcgcgcg
     8041 cgagtttgtg atgaaagacg cttattcctt ccacgccgac tatgcctcgc tccagacgac
     8101 ctatcaagat atgtatgatg cttattgccg catctttacc cgactaggct tggcgttccg
     8161 acccgttgct gcggataccg gcagcatcgg cggtaccggt tcgcacgagt ttcaagtgtt
     8221 ggcggaaagc ggcgaagatg tgattgcata cagcgacact tccgattacg ccgccaatat
     8281 cgagttagcg ccgaccttgc cgctcaaagg cgaacgtgca gccgctcagg cagaactcgt
     8341 caaagtacac acgccaaacg tcaaaaccat tgattcattg gttgatttcc tcagtattcc
     8401 gattgaaaaa acactgaaat ccatcgtggt tgaaggcgaa aacgaaggcg aacttatcct
     8461 gctgctgtta cgcggcgacc atgaatttaa cgacatcaaa gccgagaaac tcgcgggcgt
     8521 gaaatcgccg ctgacgatgg caagccctgc cgcgattgtc gaacagttcg gcgcaaacgg
     8581 cggctctctc ggccctgtcg gttttgcagg caaagtctat gccgatttcg ctaccgaaaa
     8641 aggcgcggac tgggtcatcg gcgcaaacga agacgactac cactataccg gcttcaattt
     8701 cggccgcgac gctgccgaac ctgagtttgt cgatttgcgc aacgtcgtcg agggcgacga
     8761 aagccccgac gggcaaggcc gtctgaaact ggcgcgcggc attgaagtcg gacacgtttt
     8821 ccaattgcgc gacaaataca cccaagccat gaacgtaagc ttccttgaca acaacggcaa
     8881 atcgcaaatt atggaaatgg gctgctacgg catcggcatc acccgcgtcg ttgccgccgc
     8941 catcgagcag aataacgacg aaaaaggcat catctggacc aaagcaatgg cgccgtttga
     9001 agtcgttatc gtgccgatga actacaaaaa atcagacacc gtacgcgaag ccgccgacaa
     9061 aatctatgcc gaattgctgg cggcaggcgc agacgtcctc ctcgacgacc gcgacgaacg
     9121 cgcaggcgtg ttgctgaacg attccgagct gttgggtatt ccgcaccgca tcgtcatcgg
     9181 cgaccgcgcc ttgaaagaag gcaatgtcga atacgccgaa cgccgcgaca acgaagcgca
     9241 ggcagttgca atcggagaaa ttgttgcacg tgtaacagct tcattaaatg cgtaagcttg
     9301 gtcgaatgcc gtctgaaaat aatttcagac ggcattttta cttaggggga aagtgaagtg
     9361 gcttcagtat ttttagaagg agaggcagtc gaggtcggca atattttctg tatcggcagg
     9421 aactatgccg cacatatcga agagttaaaa aacgaaatac cttccgaacc tgtggtgttt
     9481 atgaagccgt caggcagcat tttgaacagc ggcggtacaa tcttgctgcc cgagtttagt
     9541 agggatgtgc agtttgagtg cgaactcgtg ctgctggtcg gtaaggattc agacggcacg
     9601 ggcgagggta aagatatttt gggatgtgtc gcaggatatg gtgtagggct ggaccttacg
     9661 gcacgggata tccaatgccg tctgaaggaa aaagggctgc cctggctgaa ggcaaaaggg
     9721 ttcaggcatt ccgcgtgcgt gtcggatttt gcggcggcag gcagaatcgg caatcccgag
     9781 aaggtgttgt tttcattaaa acaaaacggc gtattgaaac agcgcgggga taccggtttg
     9841 atgatttacc cgattcggga aattttacac aaactggcgg cagattacgg tttggggaaa
     9901 ggggatttgg tgtttaccgg aacgccctcc ggcgtgggcg caatcggtgc gggggacaat
     9961 ttggcattgg agctggacgg cttggtgcgc gcctctttca cggtagggtg ttaaccgcgc
    10021 cgcttaaccc tcacgcccgt tgaaacattc cgccggaccg ccctcgaaaa aaccgtgcaa
    10081 gatggcttgc gccgccactt ggtcgagcac cgatttgcgt tttttgccga agacctgtgc
    10141 ttccgaaagc aggctttcgg catagacgga cgacagccgt tcgtcaaccc aatagacggg
    10201 gagattgaac ctgccgttca gcctgcgtcc gaacttgcgc gacaggtgcg tcatttcatg
    10261 tttcgtgccg tcggtatgca cgggcagtcc gacgacaaaa taacgcggct gccattcttg
    10321 aaccagcttg gcgattgccg cgaacttttc atcgttgctg ccgccggtaa cggtgctcaa
    10381 aggatgggat agccctaatt ccgcgtctcc ttgtgccacg ccgatacgcg cttcgccgaa
    10441 gtcgaatgcc agtgccgttc cttttggaat tttatgcatg gccggctcct gaaaacaggg
    10501 cgagcgggtc gatgccgagt ttggcgaatg cggcggcgta acggtgttcg taggggatgt
    10561 cgaacaggat gtgttcgtcg gcgggaacag tcagccacgc attgtcggca agttcgcgtt
    10621 cgagctgccc tttgctccag cttgaatagc ctatgctgat caaggctttg tcaaccgcac
    10681 cttcgcgtga aatattttca atcacgtctc gggaagaagt tagcgcgata ttgtctgaaa
    10741 cgccgatact gctttgccag ttgccgatcg gggtatgcac gacataaccg cgctcgacct
    10801 gcaccggacc gcccatcatc acgctgtcgt gctgcatccg catggggatg tttttgccgg
    10861 tggcggaaaa aatcatgtcc atcgtaatcg gagagggttt gttgatggcg atgccgagtg
    10921 cgccgtcttc atcgtgtttg cagatataga cgaccgattg tgaaaaaaac gcgtcttcca
    10981 tatcgggcat ggcgatgaga aagtggttgg agaggttcat atcagcgttt gtcgagattt
    11041 tctatcgggt tggaaatcat gcaggcgccg gcaagcaggt tgccggaagt atcggcttgc
    11101 accccatcgg tttgcggtat gccgccgccg aaacaggaaa ccgcgcttaa ggaatttttc
    11161 ggcgagcctt ggatgatttt atcgctgtac accaaatagg cgaaggtttt gcgtttgggg
    11221 tcgtaataac ggacaatctg ccggctcttg aacgcgaagc tcgcaccgtg tttgaaaact
    11281 tctttcggtt tgcgcacggc ggtttcgtca aaagaaatcg aagatgccgt ctgaacgcac
    11341 gaaaccgatg cgtcggacgc gtcctcttcc aaattgacca tttccttcaa gccgcctttt
    11401 tttgcatacg aaatataaca ggcaacccct tgaacgtcgg gatcgtcgaa tccttccact
    11461 tcgatacggt cgtttttgcc cagtatgttg aaaacggtac ttgcccgtcc gattttatcg
    11521 gtttcgccgc tgccgcaggc agtcagcagg acggcggcag acagcagtag cagtctgttc
    11581 atcaaaattt ccttatcggt tgaatccccg ttttccgtga aagcggcagg gcttgctttg
    11641 tttggagggg cgcacgttcc caaaatcggg caacatacgg tatgcggctt aatgtgctac
    11701 cctgtgtgtc ggaacacaat atccgttgcg gcattgaaaa tatgacgcac aatataaggc
    11761 tgtttgagcc gattgcaagt atccgcgcaa agaaggcgca agattgggca aacggcattt
    11821 tcagacggca taactgacag tataatccaa acgtccggct gctttcgtgc agccttcagt
    11881 attatccaac tttttccgaa ggtcgaacca tgcgctacaa accccttctg cttgccctga
    11941 tgctcgtttt ttccacgccc gccgttgccg cccacgacgc ggcacacaac cgttccgccg
    12001 aagtgaaaaa acagacgaag aacaaaaaag aacagcccga agcggcggaa ggcaaaaaag
    12061 aaaaaggcaa aaatggcgca gtgaaagata aaaaaacagg cggcaaagag gcggcaaaag
    12121 agggcaaaga gtccaaaaaa accgccaaaa accgcaaaga agcagagaag gaggcgacat
    12181 ccaggcagtc tgcgcgcaaa ggacgcgaag gggataagaa atcgaaggcg gaacacaaaa
    12241 aggcacatgg caagcccgtg tccggatcca aagaaaaaaa cgcaaaaaca cagcctgaaa
    12301 acaaacaagg caaaaaagag gcaaaaggac agggcaatcc gcgcaagggc ggcaaggcgg
    12361 aaaaagacac tgtttctgca aataaaaaag tccgttccga caagaacggc aaagcagtga
    12421 aacaggacaa aaaatacagg gaagagaaaa atgccaaaac cgattccgac gaattgaaag
    12481 ccgccgttgc cgctgccacc aatgatgtcg aaaacaaaaa agccctgctc aaacaaagcg
    12541 aaggaatgct gcttcatgtc agcaattccc tcaaacagct tcaggaagag cgtatccgcc
    12601 aagagcgtat ccgtcaggcg cgcggcaacc ttgcttccgt caaccgcaaa cagcgcgagg
    12661 cttgggacaa gttccaaaaa ctcaataccg agctgaaccg tttgaaaacg gaagtcgccg
    12721 ctacgaaagc gcagatttcc cgtttcgtat cggggaacta taaaaacagc cagccgaatg
    12781 cggttgccct gttcctgaaa aacgccgaac cgggtcagaa aaaccgcttt ttgcgttata
    12841 cgcgttatgt aaacgcctcc aatcgggaag ttgtcaagga tttggaaaaa cagcagaagg
    12901 ctttggcggt acaagagcag aaaatcaaca atgagcttgc ccgtttgaag aaaattcagg
    12961 caaacgtgca atctctgctg aaaaaacagg gtgtaaccga tgcggcggaa cagacggaaa
    13021 gccgcagaca gaatgccaaa atcgccaaag atgcccgaaa actgctggaa cagaaaggga
    13081 acgagcagca gctgaacaag ctcttgagca atttggagaa gaaaaaggcc gaacaccgca
    13141 ttcaggatgc ggaagcaaaa agaaaattgg ctgaagccag actggcggca gccgaaaaag
    13201 ccagaaaaga agcggcgcag cagaaggctg aagcacgacg tgcggaaatg tccaacctga
    13261 ccgccgaaga caggaacatc caagcgcctt cggttatggg tatcggcagt gccgacggtt
    13321 tcagccgcat gcaaggacgt ttgaaaaaac cggttgacgg tgtgccgacc ggacttttcg
    13381 ggcagaaccg gagcggcggc gatatttgga aaggcgtgtt ctattccact gcaccggcaa
    13441 cggttgaaag cattgcgccg ggaacggtaa gctatgcgga cgagttggac ggctacggca
    13501 aagtggtcgt ggtcgatcac ggcgagaact acatcagcat ctatgccggt ttgagcgaaa
    13561 tttccgtcgg caagggttat atggtcgcgg caggaagcaa aatcggctcg agcgggtcgc
    13621 tgccggacgg ggaagagggg ctttacctgc aaatacgtta tcaaggtcag gtattgaacc
    13681 cttcgagctg gatacgttga tcggattgcc gaaacggtag gctttatgca aatcgtgccg
    13741 atatggtgcg ctgttccgtg aagtttgccg tttgccgtac ctgtattttc cggtttttgg
    13801 aaatcaagag agaaagttaa agaagaatgt cgaaacctgt ttttaagaaa atcgcacttt
    13861 atactttggg tgcaatcagc ggcgtggccg tcagtctggc ggtgcagggt tttgccgccg
    13921 agaaggacag gcgggataac gaagtcctgc cggtgcaatc catccgcaca atggcggagg
    13981 tttacggtca aatcaaggca aactactatc aggacaaacc cgatgccgat ttgtttgaag
    14041 gtgcgatgaa gggtatggtg gccggtttgg atccgcattc cgaatatatg gataaaaaag
    14101 gttatgccga gataaaggag tccaccagcg gcgaatttgg cggcttgggg atggaaatcg
    14161 ggcaggaaga cggatttgtc aaagtggttt cgccgattga ggacacgcct gcggaacggg
    14221 cgggggtgaa aagcggcgat ttcattgtga aaatcgataa tgtttcgaca cgcggcatga
    14281 cggtcagcga agcggtgaag aaaatgcggg gcaagccggg tacgaagatt actttgacgc
    14341 tgtcgcgcaa aaatgccgac aagccgatag tcgtcaacct gacccgtgcc attattaaag
    14401 tgaaaagcgt ccgccatcac ctgatcgaac ccgattacgg ctatatccgc gtgtcgcagt
    14461 tccaagagcg gacggtcgaa agcgtcaata ccgccgcaaa agagctggta aaggaaaata
    14521 aaggaaaacc gctcaagggg ctggtgttgg atttgcgcga cgaccccggc gggcttttga
    14581 ctggcgcggt cggcgtgtcg gcggcatttc tgccgtctga agcagtcgtc gtcagcacca
    14641 agggacgcga cggcaaagac cgcatggtac tgaaagccat tcctgaagat tatgtgtacg
    14701 ggatgggcgg cgattcgttg gcgggcattc ctgccgagtt gaaaaccata ccgatgacgg
    14761 tattggtcaa ttccggttcg gcttccgcgt cggagattgt cgcaggtgca ttgcaggatc
    14821 ataaacgcgc ggtcatcgtc ggtacgcaga gcttcggcaa aggttcggtt cagactttga
    14881 ttcctttgtc caacggcagc gcggtcaagc tgacaacggc actgtattat acgccgaacg
    14941 accgttctat tcaggcgcag gggattgttc ccgatgtcga agtaaaagat aaggaacgca
    15001 tttttgaaag ccgcgaggcg gatttggtcg gacacatcgg caatcccttg ggcggcgagg
    15061 atgtgaacgg tgaaaccctt gccgtgccgc ttgaaaaaga tgcggataag cccgctgtaa
    15121 aagaaaaagg taaaaagaaa aaggacgagg atttgtcttc aaggcggatt cccaaccctg
    15181 ccaaagacga ccagttgcgg aaagctttgg atttagtcaa gtcgcccgag cagtggcaga
    15241 agtctttggg gctggcggcg aaaaagccgg tttcaaataa agataagaaa gataaaaaag
    15301 ataagaagta ggttttgacg atgccgtctg aaaggatttc agacggcata ttatgtttga
    15361 aaggacaggt tatgagcgat tttttaccgg aatttgaacc gtgcgg
//
LOCUS       DAWGRZ010000033        15303 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000033 DAWGRZ010000000
VERSION     DAWGRZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15303
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.033"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..140)
                     /locus_tag="WHT44_000709"
     CDS             complement(<1..140)
                     /locus_tag="WHT44_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ2532927.1"
                     /translation="MSVQTVTYNKFFMNTNQPAVYDPLTRALHWLTVAGFIGILTTIV
                     LWT"
     gene            382..1536
                     /gene="obgE"
                     /locus_tag="WHT44_000710"
     CDS             382..1536
                     /gene="obgE"
                     /locus_tag="WHT44_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ2532928.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTG
                     ETVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1619..2668
                     /locus_tag="WHT44_000711"
     CDS             1619..2668
                     /locus_tag="WHT44_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ2532929.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3356..4777
                     /locus_tag="WHT44_000712"
     CDS             3356..4777
                     /locus_tag="WHT44_000712"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ2532930.1"
                     /translation="MTTIYNTLTRQKEPFSPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGKLTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPPADPMPSEAGDDYTRRFYVAMNDDFDTVKAVAVLFELAGEVNKTNDAQL
                     AGRLKALGGIIGLLQRDPTEFLQGGAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4860..5639
                     /gene="xth"
                     /locus_tag="WHT44_000713"
     CDS             4860..5639
                     /gene="xth"
                     /locus_tag="WHT44_000713"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ2532931.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            5626..5970
                     /locus_tag="WHT44_000714"
     CDS             5626..5970
                     /locus_tag="WHT44_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532932.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPMLVWAIFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIASKCLGWKGWRRVVNIFACLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            5981..6511
                     /locus_tag="WHT44_000715"
     CDS             5981..6511
                     /locus_tag="WHT44_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ2532933.1"
                     /translation="MQGFELEDLTLWLIRDAGEDQMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHAFQRVRCPCRAALVVPEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6739..7854)
                     /gene="asd"
                     /locus_tag="WHT44_000716"
     CDS             complement(6739..7854)
                     /gene="asd"
                     /locus_tag="WHT44_000716"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ2532934.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIVLDPVNRDVLDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADALADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            8030..8611
                     /locus_tag="WHT44_000717"
     CDS             8030..8611
                     /locus_tag="WHT44_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ2532935.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGSGEEIFGLLLGMTSFWVGLAGILAYTVIQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(8673..9527)
                     /gene="folD"
                     /locus_tag="WHT44_000718"
     CDS             complement(8673..9527)
                     /gene="folD"
                     /locus_tag="WHT44_000718"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ2532936.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQNNLHTPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            9750..9827
                     /locus_tag="WHT44_000719"
     tRNA            9750..9827
                     /locus_tag="WHT44_000719"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9785..9787,aa:Pro,seq:tgg)
     gene            9852..9928
                     /locus_tag="WHT44_000720"
     tRNA            9852..9928
                     /locus_tag="WHT44_000720"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9886..9888,aa:Arg,seq:tct)
     gene            9956..10031
                     /locus_tag="WHT44_000721"
     tRNA            9956..10031
                     /locus_tag="WHT44_000721"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9989..9991,aa:His,seq:gtg)
     gene            10167..10673
                     /gene="rfaE2"
                     /locus_tag="WHT44_000722"
     CDS             10167..10673
                     /gene="rfaE2"
                     /locus_tag="WHT44_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEZ2532937.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGDALVLALNTDASVRRLGKGGDRPVNPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            10670..12448
                     /locus_tag="WHT44_000723"
     CDS             10670..12448
                     /locus_tag="WHT44_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ2532938.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIYGLLNMIAAEGREYEHI"
     gene            12460..13326
                     /locus_tag="WHT44_000724"
     CDS             12460..13326
                     /locus_tag="WHT44_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ2532939.1"
                     /translation="MKWLFILLVAINIAVFGGTVGYKLTLKQAGRIPEAQNAANNLQV
                     QPVAPTMPVVRNIPASGPVVQAASESDTGALLKQGDILSEEQAEQLRLKKEAEQKKLK
                     EKKQREEKARREKLAAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHV
                     ASRSVEKRTAQAKPADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSR
                     ENAQALQNRLAGAGFGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKIT
                     SLYGKLNLKSCK"
     gene            complement(14089..14877)
                     /locus_tag="WHT44_000725"
     CDS             complement(14089..14877)
                     /locus_tag="WHT44_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ2532940.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSIQTAQPAMITVSLRR
                     AGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(15091..15285)
                     /gene="thiS"
                     /locus_tag="WHT44_000726"
     CDS             complement(15091..15285)
                     /gene="thiS"
                     /locus_tag="WHT44_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ2532941.1"
                     /translation="MNIILNGGPAELHGTTVADLIAQTAPQKPFAVAVNTVFVPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT         3665 a         3849 c         4249 g         3540 t
ORIGIN      
        1 gtccacagga caatggtggt cagaatgccg atgaagccgg caacggtcag ccagtgcagc
       61 gcgcgtgtca acgggtcgta aacggcaggt tggtttgtgt tcataaaaaa cttattatat
      121 gtaacggttt gaacggacat tccgtcgggt cggaatgtca aaaaggcgcg attgtaccaa
      181 agaagttgga cataattttg tttgcaggct gaagatttgc ttaaaaattc attaagatgg
      241 gcggaacaaa tagtttgggt acggcgtgtt gaaataccgc ttatccctta taatagcgtc
      301 cgaaattctg ttcggacggc atcaaaacac acggtaatcc gtctgaaccc cccatttgaa
      361 accaacaaac aagagcattg aatgaaattc atcgacgaag caaaaatcga agtcgccgca
      421 ggcaaaggcg gtaatggcgc aaccagtttc cgccgcgaaa aattcgtacc gcgcggcggc
      481 ccggacggcg gcgacggcgg caaaggcggc agcgtctggg cagaagccga cgaaaacacc
      541 aacaccctcg tcgaataccg cttcgttaaa cgctaccaag ccaaaaacgg cgaaaaaggc
      601 cacggttccg accgttacgg cgcaggtgcg gacgacatcg tcctcaaaat gcccgtcggc
      661 acccttatcc gcgacctcga caccggcgaa accgttgccg acctcactta tcacggacag
      721 cgcgtctgcc tcgccaaagg cggcaaaggc ggcttgggca acatccactt caaatcgtcc
      781 gtcaaccgcg ccccgaaaca atccaccccc ggcgaagaag gcgaagcccg ttccctgcaa
      841 ctcgaactta aagtcctcgc cgatgtcggc ttattgggta tgcccaacgc cggcaaatcc
      901 accctgatta ccgccgtatc cgccgcacgc cccaaaatcg ccaactaccc cttcaccacc
      961 ctgcatccaa acttaggcgt ggtacgcatc gacgaaaacc acagcttcgt gatggccgac
     1021 atccccggcc tgattgaagg cgcggcagaa ggcgcaggcc tcggccatcg tttcctcaaa
     1081 cacttatcac gtaccggcct gctgctgcac gtcgtcgatt tggcgccctt cgacgaaacc
     1141 gtcaaccccg ccgaagaagc actcgccatc atcaacgaat tacgcaaata cgacgaagaa
     1201 ctctacggca aaccgcgctg gctggtactg aacaaactcg atatgcttga cgaagaagaa
     1261 gcccaaacgc gcactgccgc cttcctcgaa gccgtcggct gggactaccc caaacccgac
     1321 gaccgtttcc aatttgacat ggaaaccccg cgcctcttcc aaatcagtgc gctgacccac
     1381 caaggcacgc aggaattggt acaccaaatc aaccaatacc tgaccgagaa aaaacgtatc
     1441 gaagctgaaa aagcagaagc ggagaaagca gcggcaaatg ttgaaattat cgaacagcag
     1501 cctaaaacgg atacaggcgt gtttaagccg gagtgaaaaa agaaagggtt tcagacgata
     1561 tttgttctac aaaaggttgt ttgaaacaat tttaaagcaa gattgaaatg gattaaaaat
     1621 gtcatctaac caatatatcc agtcgcttga gttgacaaac tttaccgttc ttccaaacga
     1681 tacttttgaa ttttctgaaa atctcaatgt tattgttgcc gaaaacggtt gtggcaaaac
     1741 acatttgtta aaaattttat acagcctgct agaagtaaca tctaacacca aaaacagatt
     1801 actgaaaaca gaattgcaaa aaagttttgc cgataaatta ctgaatgttt tccgtcccga
     1861 ttccttgggt aggttgagca aacgtttgca ggggcgcggg cgtacggaaa tcgttttaaa
     1921 actgcaaaac ggcactacgc atagtagtct gaatttttcc agtaactcat catcgcaagt
     1981 caatgttcaa agcatcggat tgaaagaaaa tgaatatact ccgacaccta tcttcttacc
     2041 cagccgcgaa ttaattacac tttgcccttg gttcacttct ttgtatcaaa accaaagcat
     2101 ccctttcgaa gaaacttggt ttgatacctg tatgcaatta aatcacccgc ttgccaaagg
     2161 ccccagagaa acaaaaattc gagaattgct cgaacctatt gagaacgcga tgggcggaaa
     2221 agtatctgaa gaacaaggaa ggttttatct atcgctgaca aatacgggcg gtaagattga
     2281 agcacctttg gtagcagagg gtttgcggaa atttgtcatg attgcccggt taatcgcaac
     2341 aggggcattg ctggataaag gctatctatt ttgggatgaa ccggaagcta atctgaatcc
     2401 taaattgatc aaagtggctg cccgtattat ttggtcttta tctcaacagg gtattcaggt
     2461 ttttattgca acacactctt tattcttatt gagagaattg gaacttttga agcttgaaaa
     2521 taacacggat ttacctgtac gttttttctc gttgattgct tccgatgatg gaacaaaagt
     2581 ggagcaaggg aacagtatta atgatttgaa cacattgata ttgttagatg aaaacattat
     2641 gcaggcagac aggtatctga atatttgagg ttaagctatg actacaatca cagaaaagag
     2701 attaaccttt gcttttcctg aagattatta cgttaccaag tatgacgaat gggaacatta
     2761 taaaattttt cagaacagtt gtaatttacg taataaaatt gatacaaatg aaaagggaaa
     2821 gaacgggata aatcaaagtg tagatgacga taatggcagc tcaggtgtag atataatagc
     2881 ccttcatgaa tcgacacttt ggctaatcga aattaaagat tattaccgac ttgggcttga
     2941 gccaaatgcc caatcaatag atgaaaaact ttctgaccta ccatatttaa tcgcaagaaa
     3001 aattcgagat tcattggcag gattggtttc tgctaaattt aaagcagaaa aacaggaaga
     3061 aaaagatttt tctcgcttgg ctttagattg taacgaaata aaaatagtcc ttcatattga
     3121 aatgcctagt ataagaagta aactttatcc ctcgtcatca gatttagcaa acttattaaa
     3181 agataagttt aaattatcag aatttacaaa aaattttgca aactgttatg cagaaccaat
     3241 tttcacaaat atcagccata ttaataatcc gcaactacgt aatgttcctt ggtccgtatc
     3301 tacaggaaca gaacagaaat tatcatccga gcaacaaaga ttaatccata atcccatgac
     3361 caccatctac aacaccctaa cccgtcaaaa agaacccttt tcccccatcg accctgaaaa
     3421 cgtgcgtatg tacgtctgcg gtatgaccgt ttacgattac tgccatttgg gacacgcccg
     3481 cgtgatggtg gtgttcgaca tgattgcccg ttggttgcgc gagtgcggct atccgctcac
     3541 ttatgtacgc aacatcaccg acatcgacga taaaatcatt gcccgcgcgg ctgaaaacgg
     3601 cgagaccatc ggcaaactga ctgcgcgttt catccaagcc atgcacgaag atgccgatgc
     3661 tttgggcgtg ttgcgtccgg atattgagcc gaaggcaacg gaaaacattc cgcaaatgat
     3721 tgccatgatt gaaaccctga ttcaaaacgg caaggcatat cctgccgcaa atggcgacgt
     3781 ttactacgcc gtgcgcgagt ttgccgctta cggacaattg tcgggcaaat cgttggacga
     3841 cctgcgtgcg ggcgaacgcg tggaagtgga cggtttcaaa cgcgatccgc ttgattttgt
     3901 gttgtggaaa gccgccaaag caggcgaacc ggcgtgggaa agcccgtggg gcaacggccg
     3961 tccgggttgg cacattgaat gctctgccat gagtgaaaac ctgttcggcg ataccttcga
     4021 catccacggc ggcggcgcgg atttgcagtt tccgcaccac gaaaacgaaa ttgcccaaag
     4081 cgtcggcgcg acggggcata cctgcggtca tcatcacgcg caaacccacc acgggcaaag
     4141 catcgccagc cacgtcaaat actggctgca caacggcttt atccgcgtgg acggcgaaaa
     4201 gatgtccaaa tcgctgggca acttcttcac catccgcgaa gtgttgaaac aatacgatcc
     4261 ggaagtcgtg cgcttcttca tcctgcgcgc ccactaccgc agcccgttga actattccga
     4321 cgcgcatttg gatgatgcaa aaggcgcgtt gacgcgtttg tatacgactt tgaaaaatac
     4381 gccgcccgcc gatccgatgc cgtctgaagc cggcgatgac tacacgcgcc gcttttatgt
     4441 tgcgatgaat gatgattttg atacggtgaa agcggtggcg gtgttgttcg aacttgccgg
     4501 cgaagtgaac aaaaccaatg acgcgcaact ggcaggccgt ctgaaagcct tgggcggcat
     4561 catcggactg ctgcaacgcg atccgaccga gttcctgcaa ggcggcgcgg cttcagacgg
     4621 tctctccaac gaagaaatcg aagacttgat cgcccggcgg aaacaggcgc gcgccgataa
     4681 aaactgggcg gaatccgacc gcatccgcga ccttctgaac gaacacaaaa tcattttgga
     4741 agacaacgcc ggcggcacga cttggcggcg cggttaaaat ccatcgaaaa gggtaaatgt
     4801 gccgcatttt gccctttttt gttcagacgg catttttatt gtgagaactt atttttatta
     4861 tgcttaaaat catttccgcc aacgtcaacg gcatccgctc cgcctacaaa aaaggttttt
     4921 acgaatacat cgccgcatcg ggcgcggaca ttgtctgcgt gcaggaactc aaagcgcagg
     4981 aggctgattt gtctgccgat atgaaaaatc cgcacgggat gcacggtcat tggcattgcg
     5041 ccgagaagcg cggttacagc ggcgtggcgg tgtacagcaa acgcaaaccc gacaatgtgc
     5101 aaatcggtat gggcattgaa gaattcgaca gggaagggcg gtttgtgcgt tgcgatttcg
     5161 gcaggttgag cgttatctcg ctttatttgc ccagcggcag cagcgcggaa gaacgccagc
     5221 aggtgaaata ccgtttcctt gatgcgtttt accctatgct cgaagcgatg aaaaacgaag
     5281 ggcgcgacat tgtcgtctgc ggcgactgga acatcgccca ccaaaacatc gacctgaaaa
     5341 actggaaagg caaccagaaa aattcaggtt tcctgcctga agagcgcgag tggataggca
     5401 aggttatcca caagctcggt tggacggata tgtggcgcac gctttatccc gatgtgccgg
     5461 gctacacttg gtggagcaac cgagggcagg cgtatgcgaa agatgtcggg tggcgcatcg
     5521 attatcagat ggttacgccc gaacttgctg ccaaagccgt gtccgcacac gtttataaag
     5581 atgaaaaatt ttctgaccat gcgccgctgg tcgtggagta tgactatgct gccgaataag
     5641 gttttgggta aatatgattg gaatgtggac ggaaaaacag gaataggcgc ggcatgggtc
     5701 gtcgctgcgt ttatcctgcc catgttggtg tgggcgatat ttatgttgtc gcggatgcaa
     5761 ggctggcttg cgccgaccaa ggcaaacccg atatgggcgt tggtgtggct gctgatttgc
     5821 ctgccctgcc tgctgattgc gtccaaatgt ttgggttgga aaggctggcg gcgggtggtg
     5881 aatatttttg cctgcctgac cgtctgcgcc atattgagcg tacccgcttc gctgctcatc
     5941 gcctttaccc tccgggacct gctgaaatag gcggggcggt atgcagggtt ttgaactcga
     6001 agatttgacg ctttggctga tacgcgatgc cggagaggat cagatgtgga tagaccgttg
     6061 ggcgatcagt tatcccgtcg tgcagatgtc cgaagcgtcg gccggtcaaa gcatagggga
     6121 atggcaggca gggcttcaga cggcatttga acgcatacgc ggcaaatacg tcgccgttgt
     6181 cgcacacggt gcgggcgcgg ccgcattttt ggcgtggctg tatcaggcag acatcctgac
     6241 acggaagaaa attgccaaca tcatccttgt accgcagcgt cccgatattt ttcccgacga
     6301 tgcggaacac gctttccaac gcgtccgctg tccctgccgt gccgcattgg ttgtacccga
     6361 acacggcggc gtgccgcacg gttgggcgca aaaacaggcg gatttgtgga acgcccgcct
     6421 gttggtttcc ccgcattcgg gcagtttgaa cggcatgctc ggcggctggc agtggggtat
     6481 gaagctgatg caggaaatgt tgctggcgtg aacgcttgcc ttatcggaat ttcggctgta
     6541 gcaggaagtc cgcaatcggg ctgttttcat tgacgtgcag gcgcatcagg ttcggataaa
     6601 gtcgggctgc cgcataccgc cggaaatgga atcctttatt ttttacgtcg gcagtgccgg
     6661 cagggcgtta tccggcaggg ttgcaaggaa aatgccgtct gaaacaggct tcagacggca
     6721 tttccattca aacagggctc acaggctgcc caacacgata cgcaatacgc ggcgcagcgg
     6781 ttcggcagcg ccccacaaaa gttggtcgcc gacggtgaac gcgctgatgt attcgccgcc
     6841 catgcccagt ttgcggatgc gtccgacagg gacggacagc gtgccggtaa ctttggcagg
     6901 agtcagctcg tggatgctgg cttctttttc attggggatg actttcaccc agtcattcgc
     6961 gcctgccaaa atcgtttcga tttcggaaac aggcaggtct tttttcaact tcagagtgat
     7021 ggcttggctg tggcagcgca tcgcgccgac gcggacgcac aggccgtcaa tcacggttgg
     7081 attgtcgctg cggccgagga ttttgttggt ttccacgccg cctttccatt cttctttgga
     7141 ctggccgttg cccaaatcca cgtcaatcca cggaatcagg ctgccggcga gcggtacgcc
     7201 gaagttggct ttcggatagt cttcgctgcg caggaaatcg gatactttgc ggtcgatgtc
     7261 gagaatcgag ccggcaggat cggcaagcgc gtccgccact tgggcgtgaa ccgcgcccat
     7321 accgctgatg agttcgcgca tgtttttcgc gcccgcgccg gaagcggctt ggtaggtcat
     7381 gctggttgcc cattcgacca aatcgttttg gaacaggccg cccaaagcca tcagcatcag
     7441 ggaaacggtg cagttgccgc caatgtagtt tttcacgccg tttttgagac cgttgtcgag
     7501 gacatcgcgg ttgacagggt cgaggacgat aatcgcgtcg tctttcatgc gcagtgagga
     7561 cgccgcgtca atccagtagc cgttccagcc gctgtcgcgc agggcttgga agacggattt
     7621 ggtgtaatcg ccgccttggc aggtaacgat gatgtccatt ttggcgagtt cggcaacatt
     7681 gttggcatct aataatgttt tagccgcctg accgaaatca ggggctgcgc cgccgacgtt
     7741 ggaagtggta aagaaaaacg cttcaggaat gtgggcgaag tcgttttctt ctttcatacg
     7801 ctgcatcaaa accgaaccga ccataccgcg ccagccgacg aaacctactt tcattgtgtt
     7861 gctccttgag gaaaaatcgt taataaaatt atgtatggcg taaaggaagc gttttaacgc
     7921 cgtattggga aaatgtaaag ttctttatcg ggatttcttt aagtattggc gttttcggtg
     7981 tcgtggcggt agaatcgggg tcagttaaga acaattatca ggaacggata tggaagaaaa
     8041 aaacgactac accgacgcgg catccgacaa ccgcaacggg caggaaattg aagtcggaat
     8101 tgccggtgca ggcgaccgga tacttgccgc gctgctgaac caattgttta cctttttggt
     8161 tttgttggtg ccgtttgccg gactgattgc tttcgccatc aagaatgaag gcaggattgg
     8221 cagcggcgaa gaaatattcg ggctgctttt gggcatgact tccttttggg taggactggc
     8281 aggcattttg gcgtacaccg tcatccaaat ctactatatg agccgggacg ggcagtcatt
     8341 gggtaagaaa atcatgagaa tccgtgtgtt gaaaaccgac ggccgcaatc ccggttttgt
     8401 cggcacggtt ttggtacgcg aaatcgcatg gtcggttttg gttgccatta ttgccgccgt
     8461 tatcggtctt gcagtaggtg acaacggaga aaacgccatc aacctgctgg cattccttgc
     8521 caactttgtc ctgctcttta tggtcaaacg cgaccgccgc acgctttacg acatactggc
     8581 ggatacggtt gtcgtcaagc tgcccaaata aacggacacg gcaaaatgcc gtctgaaagc
     8641 ctttcagacg gcatttttat cttcagaacc gctcaagcat cgtgcagtga agccgcgtgc
     8701 agggtgtttt ccatcaatgt ggcaatcgtc atcggaccca cgccgccggg aacgggcgta
     8761 atcatcgccg cccgttcttt tgccgtttca aattccacgt cgccgcacag gctgccatcg
     8821 tccaaacggt tgatgcccac atcaataacg accgcgccag gtttgatcca ttcgcctttg
     8881 acaaagttcg gaatgcctac gccgaccacc aaaatatcgg ctccggcaac ctcgtctgtc
     8941 agattttcgg ttgcgctgtg gcagaccgtt accgttgcgc gcgccagcag cagttccaaa
     9001 gcctgcgggc ggccgacgat attcgacgcg ccgaccacga ccgctttttt ccccttcgga
     9061 tcaatgccgt aagcttccaa aagcgtcatc acgcccttgg gcgtacacgg gcgcatcagc
     9121 ggcattttga ccgccagcct gccgacattg taaggatgga agccgtccac gtccttatcc
     9181 ggcgaaatac gttccaaaac cgcctggctg tcgaggtgct tcggcagcgg tagctgaacc
     9241 agaataccgt ccacttcgga atcggcattc aggcggtcga ccagtgccag cagttcttcc
     9301 tgcgatgttg attcgggcag ctcgtaagac agtgatttga tgccgcattt ttggcaggca
     9361 gttttcttgt tgcggacata aaccgcgctg gcagggtcgc ctccgaccaa aaccacggcc
     9421 aggcaaggcg tgtgcagatt gttctgttgg cgttgcgcca ccgcttcggc aaccgcctgc
     9481 aggcgttttt gcgaaacttc tttaccattg atcagttgtg ccgacatatc cgtccttttg
     9541 ttaaacaggt agaaaatgct gaaaattatc gcattttccg tttgttttta cagcaggatg
     9601 ccgaaaataa atttgccctg aaataaaata cggctgtttg gacggattaa atattttttc
     9661 aaatcaaaaa actatatttt tcatcttggt gtcttgaaaa taaatgttga cgcatctttc
     9721 cctgagttgt atagttcgct tcttcaagtc ggggcgtagc gcagcccggt tagcgcatct
     9781 gctttgggag cagagggtcg tgagttcgaa tcccaccgcc ccgaccaatc aatcagcacg
     9841 aatcggcatg agcgcccgta gctcaaccgg atagagcacc gaccttctaa gtcgggggtt
     9901 acaggttcga ttcctgtcgg gcgtgccaag aatttgattg aaaaccgttt ttatggtggc
     9961 tgtagctcag ttggtagagc cccggattgt gattccggtt gtcgtgggtt cgagccccat
    10021 cagccacccc agataaaggc ccgtactttt cggtacgggt cttttcccgt ttgcgcgtac
    10081 tgtccgcgcc gccggctttg cctttcgcat atgcttctgt atcatgtggg tttttctgtc
    10141 ttttatggtt tttgtcggag gctttgatgg ttgacgcttg gtctgtccct gattttgaat
    10201 cgaaaatctg tccgcccgag gcgttggcgg cgcgtttggc gttgttgccg cgcccgctgg
    10261 tgtttaccaa cggctgtttc gacatcctcc acagggggca cgttacttat ctggcgcagg
    10321 cgcgttcgat gggggacgcg ttggtgctgg cgttgaatac cgatgcttcg gtgcggcgtc
    10381 tgggcaaggg cggtgaccgc ccggttaatc ctttggagaa ccgtgccgcc gttgccgccg
    10441 ctttggaaag tgtggatttg gtaacgtggt ttgacgggga tacgccggcg gcgttgattg
    10501 aggcggtcaa acctgagatt ttggtcaagg gcggcgattg ggctgcggat aagattgtcg
    10561 gtgcggcaga aacgttggcg cgcggcggtc aggtgttttc aattccgttt ctgcaccaga
    10621 cttcgacaac gaagactttg gcaaaaatcc gtgcggcaga gggcggaaaa tgacggtttt
    10681 gaagctttcg cactggcggg tgttggcgga gcttgccgac ggtttgccgc aacacgtctc
    10741 gcaactggcg cgtatggcgg atatgaagcc gcagcagctc aacggttttt ggcagcagat
    10801 gccggcgcac atacgcgggc tgttgcgcca acacgacggc tattggcggc tggtgcgccc
    10861 attggcggtt ttcgatgccg aaggtttgcg cgagctgggg gaaaggtcgg gttttcagac
    10921 ggcattgaag cacgagtgcg cgtccagcaa cgacgagata ctggaattgg cgcggattgc
    10981 gccggacaag gcgcacaaaa ccatatgcgt gacccacctg caaagtaagg gcagggggcg
    11041 gcaggggcgg aagtggtcgc accgtttggg cgagtgtctg atgttcagtt ttggctgggt
    11101 gtttgaccgg ccgcagtatg agttgggttc gctgtcgcct gttgcggcag tggcgtgtcg
    11161 gcgcgccttg tcgcgtttag gtttggatgt gcagattaag tggcccaatg atttggttgt
    11221 cggacgcgac aaattgggcg gcattctgat tgaaacggtc aggacgggcg gcaaaacggt
    11281 tgccgtggtc ggtatcggca tcaattttgt cctgcccaag gaagtagaaa atgccgcttc
    11341 cgtgcaatcg ctgtttcaga cggcatcgcg gcggggcaat gccgatgccg ccgtgctgct
    11401 ggaaacgctg ttggtggaac tggacgcggt gttgttgcaa tatgcgcggg acggatttgc
    11461 gccttttgtg gcggaatatc aggctgccaa ccgcgaccac ggcaaggcgg tattgctgtt
    11521 gcgcgacggc gaaaccgtgt tcgaaggcac ggttaaaggc gtggacggac aaggcgtttt
    11581 gcacttggaa acggcagagg gcaaacagac ggtcgtcagc ggcgaaatca gcctgcggtc
    11641 cgacgacagg ccggtttccg tgccgaagcg gcgggattcg gaacgttttc tgctgttgga
    11701 cggcggcaac agccggctca agtgggcgtg ggtggaaaac ggcacgttcg caaccgtcgg
    11761 tagcgcgccg taccgcgatt tgtcgccttt gggcgcggag tgggcggaaa aggcggatgg
    11821 aaatgtccgc atcgtcggtt gcgctgtgtg cggagaattc aaaaaggcac aagtgcagga
    11881 acagctcgcc cgaaaaatcg agtggctgcc gtcttccgca caggctttgg gcatacgcaa
    11941 ccactaccgc caccccgaag aacacggttc cgaccgctgg ttcaacgcct tgggcagccg
    12001 ccgcttcagc cgcaacgcct gcgtcgtcgt cagttgcggc acggcggtaa cggttgacgc
    12061 gctcaccgat gacggacatt atctcggggg aaccatcatg cccggtttcc acctgatgaa
    12121 agaatcgctc gccgtccgaa ccgccaacct caaccggcac gccggtaagc gttatccttt
    12181 cccgaccaca acgggcaatg ccgtcgccag cggcatgatg gatgcggttt gcggctcggt
    12241 tatgatgatg cacgggcgtt tgaaagaaaa aaccggggcg ggcaagcctg tcgatgtcat
    12301 cattaccggc ggcggcgcgg caaaagttgc cgaagccctg ccgcctgcat ttttggcgga
    12361 aaataccgtg cgcgtggcgg acaacctcgt catttacggg ttgttgaaca tgattgccgc
    12421 cgaaggcagg gaatatgaac atatttaagg aatacagaga tgaaatggct atttatcctt
    12481 ttggttgcga ttaatattgc cgtattcggc ggtacggtag gttacaaact gacactgaaa
    12541 caggccggca gaataccgga ggcacagaat gccgcaaaca atttgcaggt tcaaccagtt
    12601 gccccaacta tgccggttgt tcggaatatt ccagcatccg gtcctgtcgt tcaggcggca
    12661 tctgaatcgg atacaggcgc actgctcaaa cagggcgaca ttctgagcga agaacaggcg
    12721 gagcagttgc gcttgaaaaa agaagcggaa cagaaaaaac tgaaagagaa aaaacagcgt
    12781 gaagaaaaag cccgccgcga aaaactcgcc gccgaaaagg cgcaggcgga acgcgaaaac
    12841 ggcgcggcgg atgccttatg cgccgcgcag gcaagcctca cgatggacga agatgactac
    12901 caccgcatca aaggactttt gggcaaatgg tcgcacgttg ccagcaggag cgtcgaaaaa
    12961 cgcaccgccc aagccaaacc tgccgacaaa acctaccgcg tcgtcctgcc cgtttccgcc
    13021 gatgccgaaa atcaggcggc ggagctgtct gccaaaggtt tcaaccccat accgtttgac
    13081 ggcgcattga gtttgggtgt cggcaacagc cgggaaaacg cccaagccct gcaaaaccgg
    13141 cttgccggcg ccggattcgg cggggcgcat attgtcgaac actttgccga agccgacagg
    13201 caggacgatt ctttgagcgt gtcgcgtatg acggttttgt ttaccggcgt gaatgccgcc
    13261 gatgcggacg aaattcgtaa aatcacgtcc ctatacggca aactgaacct caagtcttgc
    13321 aaataggcgc gaaagccgga cgataaacgc caaacccacc gcgaaggtgg gtttggcgtt
    13381 tcggttttga gtatctgata tataaaaagg gaatgtatgt ctcgtatatg ccgactccaa
    13441 gtgtgaaagt gatgatgggg aaatatggtg tttaatggtt cggaacggac ggcttccgag
    13501 tggttttgat tatacgctaa taataataat tatcaataaa aatttaaaaa taaatgcaga
    13561 ttttttgtaa tacgatgaaa aataaacaaa ttaacctgtc cgccgatgtt tgtgattcgg
    13621 gtcggcaaac tttcattgtg tcggacaaat cggggtcggc aggcatagct gagggcttcg
    13681 tgcctgacgg aaataaggtt gcccgtgttt gaacatccgt ccgatttggc gcggacaggg
    13741 ttgtcattcg ggggtgtttg agcgtggatt cggggatgcg aaagcggtaa aaccgttgcg
    13801 gatgaaggct tacggacggc tttaggtatt tttaagtgta ttcaaacaac aacaaaacgc
    13861 ttcttctatt gccgtattta gggcttgcgc ggcagcctgc acaccgtgga ggccgagtgt
    13921 ttcgttggtt gcggcatcgg gacgggcggc aaggttttcc ggccggtttt tccatttttt
    13981 ccgacaaagc cgtgatgccg gggaatggcg gtttgtccgt ttcaggccgc cgccgttttg
    14041 gaccgcgatg ccgtctgaag ccttcagacg gcatttttgc tgcctttttc aatattccgc
    14101 cgaatgccaa aacggttgtc cgactgtcgg cgtgctggct tgcgctttgt cgcgtgcttc
    14161 gaccggtccg gcttcaaatg ccagccgtcc ggattcgacg gcgagtgcga aggcgcgtgc
    14221 catattgacc ggatcgccgc tgcgggaaac ggcagtattc aaaagcacgc cgtcaaagcc
    14281 ccattccatc acttgtgccg cctgtgaggg caaacccaag cccgcgtcga taatcagcgg
    14341 cgtgtcgggc aggcgttcgc gcaggacgtt caacgcgtag gcgtgaaccg cgcccaaacc
    14401 cgtgccgatc ggggccgccc acggcatcaa cgcctgacag cccgcgtcga gcaggcggcg
    14461 gcaggcaatc aggtcttcgg tgcaataagg cagcactttg aagccgtctt taatcaggat
    14521 ttccgccgct tcgacaagct ggaacacatc cggctgcaag gtgtcgtcat ctccgatgag
    14581 ttccaatttt atccaatcgg tttcaaacac ttcgcgcgcc atttgcgccg tcgttaccgc
    14641 ttcctgcacg ctttggcagc ctgccgtgtt cggcaggacg ggaacgccgg tttcttgaag
    14701 cagcgaccaa aacccctgac cgtgcgcctc gccgccgctt cccgcgcggc gcagcgagac
    14761 ggtaatcatc gcaggctggg cggtttggat ggattgtttg aggatttcgg gggtcgggta
    14821 ggcagccgtg ccgagcagca gccgcgaggg gaaagtttcg ccgtataggg tgagcataat
    14881 gggttccttt gtatagtgga ttaacaaaaa ccagtacggc gttgcctcgc cttagctcaa
    14941 agaaaacgat tctctaaggt gctgaagcac taagtgaatc ggttccgtac tatccgtact
    15001 gtctgcggct tcgtcgcctt gtcctgattt aaagttaatc caccataacg ttgttttggg
    15061 gacaggggtc gtctgaaaag gcaaaaccgc ctagccgccg accaccggcc gcacgatatc
    15121 gattttgtcg ttttcgttta aaaccgtttc cgcatacgcg cctttgggga cgaaaacggt
    15181 gttgaccgcc acggcaaagg gcttttgcgg cgcggtttgg gcgatgaggt cggcaacggt
    15241 cgtgccgtga agttcggcgg gtccgccgtt taagatgatg ttcatggttt ctcgatgttt
    15301 ctg
//
LOCUS       DAWGRZ010000034        15130 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000034 DAWGRZ010000000
VERSION     DAWGRZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15130
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.034"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(187..2658)
                     /locus_tag="WHT44_000727"
     CDS             complement(187..2658)
                     /locus_tag="WHT44_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ2532942.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYEGEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGSDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRVGMDTPITVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRVSSGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LVVLGNAMRLHKQVDMPSEKMPSEQ"
     gene            complement(2655..3833)
                     /gene="hflX"
                     /locus_tag="WHT44_000728"
     CDS             complement(2655..3833)
                     /gene="hflX"
                     /locus_tag="WHT44_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ2532943.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSATRLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLVNLKKQRALRRKPRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(3838..5118)
                     /locus_tag="WHT44_000729"
     CDS             complement(3838..5118)
                     /locus_tag="WHT44_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ2532944.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGNGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            complement(5271..5996)
                     /locus_tag="WHT44_000730"
     CDS             complement(5271..5996)
                     /locus_tag="WHT44_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ2532945.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(6069..6746)
                     /gene="radC"
                     /locus_tag="WHT44_000731"
     CDS             complement(6069..6746)
                     /gene="radC"
                     /locus_tag="WHT44_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ2532946.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            complement(6869..8218)
                     /gene="gshA"
                     /locus_tag="WHT44_000732"
     CDS             complement(6869..8218)
                     /gene="gshA"
                     /locus_tag="WHT44_000732"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ2532947.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            8478..9887
                     /gene="leuC"
                     /locus_tag="WHT44_000733"
     CDS             8478..9887
                     /gene="leuC"
                     /locus_tag="WHT44_000733"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ2532948.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLKAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            9984..10238
                     /locus_tag="WHT44_000734"
     CDS             9984..10238
                     /locus_tag="WHT44_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532949.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            10300..10941
                     /gene="leuD"
                     /locus_tag="WHT44_000735"
     CDS             10300..10941
                     /gene="leuD"
                     /locus_tag="WHT44_000735"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ2532950.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEERVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPSGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            11116..11376
                     /gene="dcm"
                     /locus_tag="WHT44_000736"
     CDS             11116..11376
                     /gene="dcm"
                     /locus_tag="WHT44_000736"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ2532951.1"
                     /translation="MQQIKFIDLFSEMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFA"
     gene            11422..11937
                     /gene="dcm"
                     /locus_tag="WHT44_000737"
     CDS             11422..11937
                     /gene="dcm"
                     /locus_tag="WHT44_000737"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ2532952.1"
                     /translation="MARILKAKKPKGFILENVEGLVTHDRKDSTQKIGRTLTVILETL
                     EALGYYVSWKVLNAKDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPT
                     ESSPFIKKLLKKFPPSELYGKSVKDKRGGKNNIHSWDIELKGAVTEEEKQLLNILLKE
                     RRKKNGLQKSA"
     gene            11952..12386
                     /locus_tag="WHT44_000738"
     CDS             11952..12386
                     /locus_tag="WHT44_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ2532953.1"
                     /translation="MPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRI
                     KDESLPKGYNIVSGKKSFEINKILDQNDVAPTLVAMNMEHLFVVDNGGLRTLTGKEGL
                     RLFGYPDDYSFDIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            12402..12563
                     /locus_tag="WHT44_000739"
     CDS             12402..12563
                     /locus_tag="WHT44_000739"
                     /inference="COORDINATES: protein motif:HMM:NF021105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ2532954.1"
                     /translation="MIKLTAQQIFDKLLDEEKILSANGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGG"
     gene            12564..13133
                     /locus_tag="WHT44_000740"
     CDS             12564..13133
                     /locus_tag="WHT44_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ2532955.1"
                     /translation="MRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPG
                     FDIADFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWA
                     INLQVKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKK
                     KFEEAYKKHYNRSISIPRWHEIAHKYKKK"
     gene            13167..14237
                     /gene="leuB"
                     /locus_tag="WHT44_000741"
     CDS             13167..14237
                     /gene="leuB"
                     /locus_tag="WHT44_000741"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ2532956.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDASYEYAPLGG
                     EAYDEYGHPYPQFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAILYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKEYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTGD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            14400..14963
                     /locus_tag="WHT44_000742"
     CDS             14400..14963
                     /locus_tag="WHT44_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ2532957.1"
                     /translation="MKKIIIAALAAAAIGTTSAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPNIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMLKTEVCGGDFSTTIDRT
                     QWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         3631 a         4086 c         3888 g         3525 t
ORIGIN      
        1 atccatttca atatccattt aagctttcaa ttaattttct atatttttca atggcatgta
       61 tcatgctatg cttgggaaat atcatcttgg ttgatgatca agccatgtat gcaaagattg
      121 gaatatggta tttgtcgtgc taataatgcc cccgttactg ttatacgtct aaagcaacgc
      181 tgtccgtcat tgttcggacg gcattttttc agacggcatg tccacctgct tgtgaagccg
      241 catagcattg cccaatacga ccagcgaact gaagctcata cccagtgcgg caatccacgg
      301 ttggacatag cccaaaacgg caagcggtac ggcaatgata ttgtacgcgc ccgcccatat
      361 caggttttgc cggataatat ggcgggtgcg ccgcgcctga tcgagcaggt gggcgacggt
      421 acgcaaatct tcgttcaata acacaatgtc cgcgccgtcc ctcgcaatat ccgtcccgcc
      481 cgctgcggcg gcggatacgt ctgcctgcgc caaaacgggc gcgtcgttga tgccgtcgcc
      541 tatcatcagc acttttttcc cttctttttg caaggctttg acgtattcca gtttgtcctc
      601 gggcatggct tgggcgcggt agtgcgcgac acccagggcg cgtgcggttt cggcaacggc
      661 ggtttcgcgg tcgccgctga ggatgtgcag ggtcaggttt ttgcctgcca actgccgcac
      721 cgcctccgcc gcgctgtctt tcaaggggtc ggtcaggtag aacacggctt ggaaaccgct
      781 ttgactgccg aggtaaaccg cgctgccgcc gccttctgtt tgcggttctt tacctgaaat
      841 ttcggcgaca taggatgccc tgcccaatgc ccaaacctgt gtttcgccgt tgacggtcag
      901 ttgcgcgccc acgccttcgc cgatgcggtt gaggcgttgt ttaatagcga tgtcggggac
      961 gctgccgctt gaaacacggc agttgaggat ggcgcgggca aggggatgtt cggactgttg
     1021 ttctaaagcc tgcgccaccg cgagaacaaa ggcttcgtct gtgcctctca acaatgagat
     1081 acggcggacg gcgggtttgc cttgggtcag cgtgccggtt ttgtcgaaga tgatgtcggt
     1141 ggtttgggcg agggtttcga ttgcctgctt tccgccgatt aaaatgcctt cgcgcgccag
     1201 cgtaccggta gaagctgcca gcgcggtcgg cgtggcaagc gataaggcgc aggggcaggt
     1261 aatgaccagc agggcgacgg taatccacaa tgcggtgtgc gcgtcggcgt acagcgtcca
     1321 gccgatgaag acggggacgg caagcaggag ttcgccgaat atgaaagacg aggcgtattg
     1381 ttccgccaac tcggcagtgc gcggtttttg cgctaaggcg cggtcgagca ggcggacgat
     1441 gtgcgacagt cgcgtgccgc cgccggtgcg gtcggtgcgt ataatcaggg ggctttgcgt
     1501 gttgagtgtg ccggcggtta ctttttcaga cggcattttg gcgacgggca ggctctcgcc
     1561 ggtcagcata gattcgttga cggcactgct tccttccagc accgtgccgt caacggggat
     1621 ggtttcgccc ggtttgacca gcacgatatc gcccgccttc aatttgacga cagctgcctc
     1681 gcaggtttcc tgcgtatcgg ggtaatcggg catatgatgg caaaacgcag gaatcagctt
     1741 caccagcctc tcggcggcat cgcctgcctt acggcgggca atgtgttcca taaagcgtcc
     1801 gcccagcagg aaaaacagca gcatcgcgat ggattcgaaa tacatcccct gccccgcatt
     1861 tgtcgcaagg ctgtaaacgc cggcgataaa ggtcatgatg atggcgacgg taatcggcgt
     1921 atccatgccg acgcggcggt ttttcaagtc gcgcagcgcg ccttgataaa acgggacggc
     1981 gcaatagaat acgacgggca gcaccattaa aaagccgccc caatggagga tttgcaggaa
     2041 atcgggttcg atgtcgctgc cgtaaaggta ggtcggcagc gcgaacatca tcgtctgcat
     2101 catccccagc ccggcaacgg cgaggcggac gatgtattgt ttgcgttctt tttggttggc
     2161 ggcttcgatt ttttgcgcgt catagggtgc ggcggtgtag cctatctgcc tgattttcaa
     2221 cagaatgtcg gaaaggcgga ttttgccgtc gtcccagacg acgcggcagc ggtgcgtgct
     2281 gtaattgagg tcgatgcgga cgatgccgtc tgtacgcaaa agctgctgtt cgatcagcca
     2341 gacgcaggcg gcgcaggtga tgccgccgag cattaaaacc gcctcgcgcg tgccgccgtg
     2401 ggtttccaca aagtcggact ggacttcggg caggtcgtac aggcggattt ggtcgaggat
     2461 ttcttggggc ggcagctcgg ttttttgcgc gtcggcggtg cgttgtttgt aataactgcc
     2521 caagcccgcg tcaataatgc tttgtgcgac cgcctgacag ccggcgcagc aggtttctcg
     2581 gtcttcgcct tcgtagcgga cggtcagatg caggttttcg ggaacatcca gcccgcagtg
     2641 gaaacaggtt tttttcatgg catttcggtt tcgtctgtgt ttggtgcggc ggcacaagac
     2701 tcggcaatgg cttcgcgcag tgcgtctata ccggtatttt cagcaacgga aatgcggacg
     2761 gcggcaattt ttcccgcagc gtcgcgccat atgcccgtgt tttgttcttc agacggcagc
     2821 aggtcggttt tgttgtacac cttgatgcac ggaatatcgc cggcatggat ttcttgcagt
     2881 acgttttcca cgtcttcaat ctgctgtccg ctgttcgggg cggcggcatc gacgacgtgc
     2941 agcagcacat cggcttgcgc ggtttcttcc agcgtggcgg aaaatgccga aatcagtttg
     3001 tgcggcaaat cactgacgaa tccgacggta tcggtcagga taatgctgca ttcgggactg
     3061 atgtacagcc gccgcgccgt ggtgtcgagt gtggcgaaaa gctggtcttt cgcatatatg
     3121 cccgacttgg tcagccggtt gaacaggctg gatttgccga cattggtata gccgaccagc
     3181 gcaaacgttt tgattgtgcc cgattcgcgg ggcttgcggc gcagggcgcg ctgttttttg
     3241 aggttgacaa gctgtttttt caaggcattg atccgatggg cgatcaatcg gcggtcggtt
     3301 tccagtttgg tttcgccggg gcctttcatg ccgataccgc cgcgctggct ctgcagatgg
     3361 ccgtaaccgc gtatcaagcg tcccgccaaa tggctcaatt gcgccaactc gacttgcagc
     3421 ctgccttcct gcgtgcgggc gcggcgggcg aaaatcgcca gaatcagccc taccctgtcc
     3481 aatacgcggc atttcagttc tttttcaagg ttgcgttcct gcgtgggcgt gagttcgtgg
     3541 ttgaatacga ccaaatcgat gccgtctgcg gcaactgctt ctgacagctc cgccgccttg
     3601 cccgtgccga caaacagcgc ggtgtgcgga cggtcgcgtt tggcagtctc cacgcgcacg
     3661 gaatcgccgc ccgccgcttt gaccagctcg acagcttccg ccaatgccgt ctgaaaaccg
     3721 ttcagacggg tggcactgct gcccgtgcca tctttgtcca acattacgcc caccagcatg
     3781 acgcgttcgg gttgcgcttg ggtactgccg ttgcggcctg ttctgcctgt catgtattca
     3841 atcctgtaaa taaagggtta cgcctcgttc cgtttctcct tccggtgcaa gcgtgatgcg
     3901 gctgcccgcc attccgtcaa cgctgaagac gatgccgccc gcctcgtgcc ttcctatgga
     3961 caggcggctg ccgggtcgct gccggtaggt ataacgggta cacgcttcca aacaccagcg
     4021 tccgcgccag tggatgaaat atttttcgcc ttttctaacc gcaagtgtct cgccgccgtt
     4081 tacctcaaaa taagggtaat cgggttttcg ggttctaaac tgccatttcg cctgcgctcg
     4141 ccgtccgttg cggacatagt cgaataccgc cgtataaagc gtgccgtatt ccaaaggctt
     4201 gagcggaaaa agcgcgaatt ggtacgcggt cagcctgccg ttggggtcgt tgccggcggt
     4261 taaaaccctg acggggcgga tttcgttttt accctgatac agcttgaaac ttttcatcgt
     4321 aattttgcct gccgcctcgg aaaaatcaat gctggcagga ttgcccgtga tttcatattc
     4381 cggcacgggg tctggacgct cgccgtggaa ataaggcagt gcgccgttgc cgacgggata
     4441 ggctgtatag agcagctcct gtgcgggcat ggcttcgtcg gtgtacacga ccgcaccgtt
     4501 atggcaggcg ttgcggtaat atttccgtcc tgcttccggc tgatttctgc cttgggcgca
     4561 atgccgctca aacctgccgt tgccctgatt gaatacgaga acggttttac cgttttcgcg
     4621 cacaaatgcc gctcctgcct catccgtatg gcggtcaagt agggaaaggc ggtggtagat
     4681 tgcgctcatt aatccgtcca cttggcgttg ctgcgtgcgg atgtcgctgt cggacgattc
     4741 ggcggcttcc tcttccgtgc tgatgttttc atgcacgccg ttgtagagat acccggcaag
     4801 gcgtgtgcgt tcggtcagct tttgtgcggt gtagtgcgga ttgtcgggat ggtgttcgcc
     4861 gtgtccgtct tcgggattga gcgtgagata gcgtgcgtgc ctgcgggcgg aattttccaa
     4921 aaccggcgcg tgtgccagct tgtgcaaacc gatttgggcg cggatgcggt tgaggtagtg
     4981 cagcgcatcg ccgccgtccc tgatttgcgg tgcggacgga tagacaagtt cgcccgcggg
     5041 cagggattgg ttttgggtat gatagaaaac gccggccgcc gaacccaaca ggagcagccg
     5101 aataaaaagg gatttcataa tgccgtcctc ccgacgcggc aacggggtgt tttatcaaaa
     5161 cggcaaactt gccaatgcgg tttccgattg cagattgcca aaccggcaaa cctttccccg
     5221 tgccgatgcc gaacccgatg ccgtctgaac cttcagacgg catcgggtgt ttatttgtcc
     5281 acccggatgg gtgcaggttc gtattgtgtc gattcgtcgc cgtaatacag cacgccgagt
     5341 ttgatgggga tgcgtccctg cgatttgcgg tgggcgttgg aatccctcag ggaataggca
     5401 caaccgcaat attcctgctg gtagaagttt tcacgtttgc tgatttcaat catacgcgcg
     5461 ctgccgccgc ctttgcgcca gttgaaatcc caatacacca catcatcgta aggcgcggcg
     5521 gcgcggtgtc cgcagtcgtt gatttgcgcc atatttttcc agcgtgaaat gcccagcgaa
     5581 ctggtaaaga cggggaaccc gtgttcatgc gcgtattgcg ccgccttttc aaaacgcata
     5641 tcgaaacaca tggtgcagcg gatgccgcgt tccggctcaa actccatgcc tttggctttg
     5701 gcaaaccatt ctttgcggtc gttttcgtag tcgtcgtctt tatcgatgaa aggaatgccg
     5761 aacttttccg caaagcgtac gttttcctct tttcggagca tatattcttt gtgcggatgg
     5821 atattgggat tgtaaaaata aatggtgtag tcgatgccgg aggcaagcat ggcttccatc
     5881 acttcgccgc tgcacggggc gcagcaggaa tgcagcaaga cttttttatg tccgccgggc
     5941 gggacgagta tggggcggtc aatgtcggta acggtaggtt tgttttgtgt ttccattaac
     6001 ttaacaatat ctgtcgaata cggttgaagc ggctatttta tcagagccgc cgcatgtaaa
     6061 acagggtgtc agggcatcag cccgagctgc ctgaacgaac ggacggtttg cgccgttacg
     6121 ataaaatggt cgagcagcga cacatcgacc agcgacatgg cctgtgccag ccgccttgtg
     6181 aacatgatgt cttcctgcga aggttcgggc gagccgcccg gatggttgtg cgcaataatc
     6241 aggctgtcgg catattcgtc caatgccagt ttgacgattt cgcggatgta aatcgtgttt
     6301 tccgcaaccg taccgcgcga cagctctctg accgcaatca gttggttttg gcggttcagc
     6361 agcagcgcga cgctgacttc gactttttcc tgccccaaat gaaagcgtaa ataatcggca
     6421 acggtatccg gatcggacag gacgatgctc tcctgcaatt cttccgccaa tatccgccgc
     6481 ccgatttccc tgactacggc aaactgtgtg aaacttgccg tccccatccc tttgtatgcc
     6541 gacagtttgc cgacctccgc gctcatcagc ctccccaaac tgccgaactc ctgcagcaaa
     6601 taacgcgcca aatcgaccgc actcattccg cgcgtgccga cgcgcagcag gattgccaaa
     6661 agttcggcat cactcaaagc cgccgccccg cgttccaaca gcttttccct aggtctttcg
     6721 ccttccggcc attgcttgat gctcatgttt tccctttttt aaatagtctg agatgccgtc
     6781 tgaaacaaat gttcccacac gccaaatatc cgaaatgccg tctgaaccgg atttccgctt
     6841 cagacggcat ctgtatttca aaacagtctt acgccgtttc ttccaactcg atggacgccg
     6901 ccagaagcga caggcgtgcc attacgccgt aaacgtagag gcggttgtgt tcgttgtcca
     6961 cgtcgcagtc tttttcagag cgcggcatac ccagcgagtc ccattgttcg aatacgcgct
     7021 tgcaggcttc gggagcttct tgggagttgt cgccgttgcc ggtggggatg ctgttggaca
     7081 gcggaacaaa caccataccg ccggcgttga ggttttcgtc cgcaccgcgc ccttcgtgta
     7141 cgcggaaaaa gccgccgatg acgaagcggt ccatcatata gacgacgggt tcgcacaccg
     7201 cgccgtttaa ggtttcataa gtataaatcc cttcttggac aatcacttcg ctgacttcca
     7261 agccttcttt gactttcgcc attttattgc ggtttttacg gttcaatccg cgcacttcgt
     7321 cggcggattt gacgctcatc acgcccatgc cgtaagtgcc ggcatcggct ttgacgatga
     7381 cgaaaggttt gtcggtaatg cccgattcgt cgtatttggc ttgaattttc gccagcacac
     7441 gttctaccgc ttccgccaac gcgtcttcgc cttcacgccc ttggaagtcc aaaccgccga
     7501 ttttttcaaa ataagggtta atttgccatt cgtcgatgcc gattaacttg gcaaattcgg
     7561 cggtaacttg gttgtacgcg ccgaaatgat ttgttttgcg gcgcgtcgtc caaccgccgt
     7621 gcaacggcgg caaaacggtt tggctgatgc ctttgaggat gtcgggaatg ccggcagaca
     7681 aatcgttgtt caacaaaacc acgcaaggcg aaaagccgtc tgcaagatgg acgcggtcgc
     7741 gggtacgcaa taaaggttcc aacaggattt tgtcgcccaa tgcagtttca aattcggtcg
     7801 gttcggttac ttccggattc aggctgccca agcgcacttc ataccctgcc gaacgcaaaa
     7861 tctcgccgag ggcgtaaatg ttttgcaggt aaaacgtatt gcgcgtgtgg ttttcgggaa
     7921 taatcaatac ggatttcgcc gtttcgcagg cgcgttgcac cgcatcttgc gcggcaaccg
     7981 ccgccagcgg gataaagttg ggattcagat tattgaagcc gccggggaac aaattcatat
     8041 cgatagacga aattttgtaa ccggcattgc gtatatcgac cgaaccgtaa aacggcgggc
     8101 ggtgtacgct ccactgcgtg cggaaccacg cctcgatttt ggcgtgattg gacaggattt
     8161 ttgcctcaaa cgcctgaagt tgcgccgaat gttcgggcga catcaccggt aatttcatct
     8221 ttcgtctgcc tctgtgtgtt ttttgacaag atgcagatga taatgccttt gaaagaatga
     8281 cgacaagtat tctttcgtat cgaatcatgc tttagatata actgtttact gaaatggaca
     8341 atacgtctaa ttttttcgtt aaaattgttt gcaaaataac accggctcaa aaattagaca
     8401 aaatttgttg cgcggtataa agaatacgtc taaaatccgc ccaatccgca ttaccattta
     8461 tccaaaagaa caacatcatg acagcacaaa ccctctacga caaactttgg aacagccacg
     8521 tcgtccgcga agaagaagac ggcaccgtcc tgctctacat cgaccgccat ttggtgcacg
     8581 aagttaccag ccctcaggca tttgaaggct tgaaaatggc ggggcgcaag ctgtggcgca
     8641 tcgacagcgt cgtctccacc gccgaccaca acaccccgac cggcgattgg gacaaaggca
     8701 tccaagaccc gatttccaag ctgcaagtcg atactttgga caaaaacatt aaagagtttg
     8761 gcgcactcgc ctattttccg tttatggaca aaggtcaggg catcgtacac gttatgggcc
     8821 ccgaacaagg cgcgaccctg cccggtatga ccgtcgtctg cggcgactcg cacacttcca
     8881 cccacggcgc attcggcgca ctggcgcacg gcatcggcac ttccgaagtc gagcacacga
     8941 tggcgaccca gtgcattacc gcgaaaaaat ccaaatccat gctgattgcc gttgacggca
     9001 aattaaaagc gggcgttacc gccaaagacg tggcacttta catcatcggg caaatcggca
     9061 cggcgggcgg tacaggctac gccgtcgagt ttggcggcga agccatccgc agcctttcta
     9121 tggaaggccg catgacttta tgcaatatgg cgatcgaagc cggtgcgcgc tcaggcatgg
     9181 ttgccgtcga ccaaaccacc atcgactatg taaaagataa acccttcgca cccgaaggcg
     9241 aagcgtggga caaagccgtc gagtactggc gcacgctggt gtctgacgaa ggtgcggtat
     9301 tcgacaaaga ataccgtttc aacgccgaag acatcgaacc gcaagtcact tggggtacct
     9361 cgcctgaaat ggttttagac atcagcagca aagtgccgaa tcctgccgaa gaaaccgatc
     9421 cggtcaaacg cagcggtatg gaacgcgccc ttgaatacat gggcttgaaa gccggtacgc
     9481 cattaaacga aatccccgtc gacatcgtat tcatcggctc ttgcaccaac agccgcatcg
     9541 aagacttgcg cgaagccgcc gccatcgcca aagaccgcaa aaaagccgcc aacgtacagc
     9601 gcgtgttaat cgtccccggc tccggtttgg ttaaagaaca agccgaaaaa gaaggcttgg
     9661 acaaaatttt cattgaagcc ggttttgaat ggcgcgaacc gggctgttca atgtgtctcg
     9721 ccatgaacgc cgaccgcctc gcaccggggc aacgctgcgc ctccacctcc aaccgcaatt
     9781 ttgaaggccg tcaaggcaac ggcggacgta cccacctcgt cagccccgct atggcagccg
     9841 ccgccgccgt taccggccgc tttaccgaca tccgcatgat ggcgtaagta ttttcaggcg
     9901 gtctttaaga atttgacgcg gccgtctgaa atatatcccg taaggcagaa acacctttaa
     9961 tattcaaacc aaggagttta accatgaaca aacttttcat taccgccctg tccgccctcg
    10021 ccttgtccgc ctgcgccggc acttgggagg gcgcgaaaca agacaccgcc cgcaaccttg
    10081 acaaaacaca ggccgccgcc gaacgcgccg ccgaacaaac aggcaacgcc gtcgaaaaag
    10141 gctgggacaa aaccaaagaa gccgtcaaaa aaggcggcaa tgccgtcgga cgcggcattt
    10201 cccatctcgg cggaaaaatc gaaaacgcca ccgaataacc gattcgcgat gccgtctgaa
    10261 accgcttcag acggcatcgt cagcacaaga cgcaaaccca tgaaagcctt taccaaaatt
    10321 accgccatcg tcgccccgct cgaccgcagc aacgtcgata ccgacgccat catccccaaa
    10381 caatttttaa aatccatcaa acgcagcggc ttcggcccca atgcctttga cgaatggcgt
    10441 tacctcgacc acggcgaacc aggcatggac aacagcaaac gcccgttgaa ccctgatttc
    10501 tcgctgaacc agccgcgtta ccaaggcgcg caaatcctgt tgacgcgtaa aaacttcggt
    10561 tgcggctctt cacgcgaaca cgccccttgg gcattggacg actacggctt ccgcgccgtc
    10621 atcgccccca gcttcgccga catcttcttt aacaactgct acaaaaacgg ccttttacct
    10681 atcgtgttga ccgaagaacg ggttgaccga cttttcaaag aagttgaagc caacgaaggc
    10741 tatcagctct ccatcgacct tgccgaacaa accctgacca caccaagcgg cgaaacgttc
    10801 acatttgaca ttaccgaaca ccgcaaacac tgcctcttaa acggcttgga cgaaatcggt
    10861 ctgaccctgc aacacgccga cgaaattcac gcctttgaag aaaaacgccg ccaaagccag
    10921 ccttggctgt ttaacggtta aaacctatac cgtctgaagg ccgttcagac ggcattcctg
    10981 ataacatata attgttgcgg ctaatgtaaa cagaggggcg ataaattgct ttcatctttt
    11041 aagcgggctt agcgacactc taaattaatg caataatgca actctccatt tcaaataaat
    11101 ttttagattt ttaatatgca acaaattaaa tttattgact tattttccga aatgagtggc
    11161 atcaggaaag gattcgaaca agcctgtcgg aaacagtcgg ttgcttgcga atgtgttttt
    11221 acttcagaaa tcaaaccggc agctttggaa gtattgaagc aaaactaccc tgatgaagtg
    11281 ccgtatggag atataacgaa aattgaaaca ggggatattc ccgattttga catcctgttg
    11341 gcaggcttcc cttgtcaggc tttttctttc gcctgaaaaa gattaggctt tgaagataca
    11401 cggggaacgc ttttctttga tgtggcaagg attttaaagg caaaaaaacc aaaaggtttt
    11461 attttggaaa atgtggaagg attggtgaca cacgatagaa aagattcgac acaaaaaata
    11521 gggcgcaccc tgaccgttat tttggaaacc ttggaagcat tgggctacta tgtttcttgg
    11581 aaagttttaa atgcaaaaga tttcggcatt ccccaaaaca ggaagcgcat ttatctgaca
    11641 ggcagtttga aatccaaacc cgacttatct ttcgaaacaa gcccaagtcc gaaattaaaa
    11701 aatattttgg aatcgggact gcctaccgaa agcagccctt tcatcaaaaa attgctaaaa
    11761 aaatttcccc cgtccgaact gtacggaaaa tcagtaaaag acaaacgggg agggaaaaac
    11821 aatattcaca gttgggatat tgaattaaaa ggcgcagtaa ccgaagaaga gaagcaattg
    11881 ttaaatatcc ttctaaaaga acggaggaaa aaaaatgggc ttcagaaatc ggcatagatt
    11941 ggatggatgg gatgcctttg acaaaagcgc aaatttcaac tttctataaa caccccgatc
    12001 tccaaaatat tttggacagc ctgacagata aaggctattt ggttttagag catccgaaac
    12061 aaaaaattgg cgggcaaaga atcaaagatg aatccctgcc caaaggctac aatattgttt
    12121 caggcaaaaa atcttttgaa atcaataaaa tattagatca aaacgatgtt gcgccaacct
    12181 tggttgcaat gaatatggag caccttttcg tcgttgacaa cggcggtttg cgtacactta
    12241 ccggaaaaga agggttgcgc ttattcggct atccggacga ttattcgttt gatattccca
    12301 aaaaagacag atgcgattta ttgggtaata ccgttgccgt ccctgtgatt aaggcggtat
    12361 ctgaaagact tctgcatact ttataaaccg acagggataa aatgataaaa cttactgcac
    12421 aacaaatatt tgataagttg ctggatgaag aaaaaatctt atcggccaat ggtcaaatta
    12481 gatttttctt aggcgatgtg gatattatcg tcaaacaaaa agatgttgtc ggcaacatca
    12541 ttcaggaatg gctcggcgga tgattgagaa aaagggaaat tgaatttgat gtttcaacca
    12601 atacccaaat gcctcccgat ttctttttaa ataaaaaaga ccgcagcaga gaattgttgg
    12661 aagtaaaggc gttcaaccga aatgccggcc cgggttttga tattgcagat tttaaaatgt
    12721 attctgatga aatcattcat aagccctata tgctggatgt agactattta atattcggtt
    12781 acgatatgga cgacaacggc aatgtaacca tcaaggattt atggcttaaa aaagtatggc
    12841 aaattacgag aagcatggat gggtgggcaa ttaatcttca agtcaaaaaa ggcgtggtgc
    12901 ataaaatccg cccgggtgtt tggtacagca taaataaaaa gaatatgcct atgtttgaat
    12961 gcttggaaga ttttgtttcc gcaattgaag aaaccgttta tcaaaacccg gcaacacggc
    13021 ataacgcatc cttatggaaa aagaaatttg aggaagccta taaaaaacac tacaaccgat
    13081 cgatttccat acctcgttgg cacgaaattg cacacaaata caaaaagaaa taacccttca
    13141 accatcaacc cataaggaaa cttgaaatga ccaaacatat cgccatcctc cgaggtgacg
    13201 gcatcggccc agaaatcgtc gccgaaaccg tccgcgtact cgacaaactc atcgcccaag
    13261 gcttggacgc cagctacgaa tacgccccat taggcggcga agcctacgac gaatacggcc
    13321 atccctatcc ccaattcacg caaaaccttt gccgtaaagc cgatgccgtc ctgcttggtg
    13381 cagtcggttc tcctcaatac gacaatctcg accgtccatt gcgtcctgag cgcggcttgt
    13441 tggccatacg taaagacttg aatctgtttg ccaatttgcg ccctgctatc ctctatcccg
    13501 agctagccaa cgcctccacg ctcaagcctg aaatcgtcgc cggtttggat attttgattg
    13561 tgcgcgaact caccggcgat atttatttcg gcgaaccgcg cggcatccgt gttttagaaa
    13621 acggcgaacg cgaaggctac aacaccatga aatacagcga aagcgaaatc cgccgtattg
    13681 cccacgttgc cttccaatcc gcccaaaaac gcagcaaaaa agtctgctcc gtcggcaaag
    13741 ccaacgtttt ggaaaccacc gaattgtggc gcgaaatctt tgaggaaatc ggcaaagaat
    13801 accccgatgt cgagctttcc catatgtacg ttgataacgc agccatgcag ctcgtgcgcg
    13861 cgcccaaaca atttgacgtg attgccaccg gcaacatctt cggcgatatt ttatcggacg
    13921 aagcctctat gctgaccggc tccatcggta tgctgccttc cgcttctttg gacgaaaacg
    13981 gcaaaggcct gtacgaaccg tcccacggct ccgcccccga catcgccgga caaaacaaag
    14041 ccaacccgct tgccaccatc ctctcgctcg caatgttgct gcgttacagc ctgaacgacg
    14101 aagcgcgcgc gcaacaagtt gaaaacgccg tacaaaaagt gctgcaacaa ggcttgcgca
    14161 ccggcgatat ttacgaagaa ggcacgaaac tcgtttcctg ctccgaaatg ggtgatgcgg
    14221 tactcgccgc cttgtaaaaa aacaaagcgc ggatcacacc gcgctttttc atccgccctt
    14281 ttccgcccgc cccgttcgtc aaccgcctgc aaacccaccg tttaccggca ggaaatgcca
    14341 taaatccctg cctatgttag gataaacgcc tttaccggcc cacacaaagg attatcatca
    14401 tgaaaaaaat catcatcgcc gcgctcgcag ccgccgccat cggcactacc tccgccgcca
    14461 cctacaaagt ggacgaatat cacgccaacg cccgtttcgc catcgaccat ttcaatacca
    14521 gcaccaacgt cggcggtttt tacggtctga ccggttccgt tgagttcgac caagcaaaac
    14581 gcgacggtaa aatcgacatc accattcccg ttgccaacct gcaaagcggt tcgcaacact
    14641 ttaccgacca cctgaaatcc gccgacatct tcgatgccgc ccaatatcct aacatccgct
    14701 tcgtttccac caaattcaac ttcaacggca aaaaactggt ttccgttgac ggcaacctga
    14761 ccatgcacgg caaaaccgcc cccgtcaaac tcaaagccga aaaattcaac tgctaccaaa
    14821 gcccgatgtt gaaaaccgaa gtttgcggcg gcgacttcag caccaccatc gaccgcaccc
    14881 aatggggcgt ggactacctc gttaacgttg gtatgaccaa aagcgtccgc atcgacatcc
    14941 aaatcgaggc agccaaacaa taaatcttga tgcgaatcag cacaaatgcc gtctgaacgt
    15001 ccttcaaacg gcatttgtgc cgtcttcatc aaaccgcccc gccaacggta agccgatatg
    15061 gcggagcaca atccgccgaa agatatttca gcggcaggca ggacttgccg gaaagtccgg
    15121 aaagtatagt
//
LOCUS       DAWGRZ010000035        14612 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000035 DAWGRZ010000000
VERSION     DAWGRZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14612
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.035"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(78..293)
                     /gene="nqrM"
                     /locus_tag="WHT44_000743"
     CDS             complement(78..293)
                     /gene="nqrM"
                     /locus_tag="WHT44_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ2532958.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(319..1374)
                     /locus_tag="WHT44_000744"
     CDS             complement(319..1374)
                     /locus_tag="WHT44_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ2532959.1"
                     /translation="MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            complement(1527..2744)
                     /gene="nqrF"
                     /locus_tag="WHT44_000745"
     CDS             complement(1527..2744)
                     /gene="nqrF"
                     /locus_tag="WHT44_000745"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ2532960.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            complement(2758..3351)
                     /gene="nqrE"
                     /locus_tag="WHT44_000746"
     CDS             complement(2758..3351)
                     /gene="nqrE"
                     /locus_tag="WHT44_000746"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ2532961.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIAEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            complement(3355..3981)
                     /locus_tag="WHT44_000747"
     CDS             complement(3355..3981)
                     /locus_tag="WHT44_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ2532962.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGIMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            complement(3981..4757)
                     /locus_tag="WHT44_000748"
     CDS             complement(3981..4757)
                     /locus_tag="WHT44_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ2532963.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKTEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            complement(4750..5982)
                     /locus_tag="WHT44_000749"
     CDS             complement(4750..5982)
                     /locus_tag="WHT44_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ2532964.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQNIANDWHYAFAN
                     ALGINMSSEAGVSDKMLFGAIYFLPIYATVFVVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            complement(5985..7328)
                     /locus_tag="WHT44_000750"
     CDS             complement(5985..7328)
                     /locus_tag="WHT44_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ2532965.1"
                     /translation="MIKIKKGLNLPIAGRPEQAVYDGPAITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFKRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVITIGRLFATGRLNTERVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDTDNRVISGSVLNGAITQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFNTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            complement(7678..9099)
                     /locus_tag="WHT44_000751"
     CDS             complement(7678..9099)
                     /locus_tag="WHT44_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ2532966.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWGLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(9114..9404)
                     /locus_tag="WHT44_000752"
     CDS             complement(9114..9404)
                     /locus_tag="WHT44_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HEZ2532967.1"
                     /translation="MQIYVKINSFFVFDRLFIKDIYLFVLNKIIYQKEHIFPSTAIRL
                     PAAFRFGKLVYPDYLLYNQSENFFQKQTGSTVRLCPGGTVPVSLPRAAQGSA"
     gene            complement(9461..9820)
                     /locus_tag="WHT44_000753"
     CDS             complement(9461..9820)
                     /locus_tag="WHT44_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ2532968.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFYRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(9817..10422)
                     /locus_tag="WHT44_000754"
     CDS             complement(9817..10422)
                     /locus_tag="WHT44_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ2532969.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDGFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGIVQIAPFHPDFQFEGTDSDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(10469..10951)
                     /locus_tag="WHT44_000755"
     CDS             complement(10469..10951)
                     /locus_tag="WHT44_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ2532970.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      11154..11251
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            11462..12562
                     /gene="gcvT"
                     /locus_tag="WHT44_000756"
     CDS             11462..12562
                     /gene="gcvT"
                     /locus_tag="WHT44_000756"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ2532971.1"
                     /translation="MTALKTTPFHQAHQDAGAKLVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQQA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLEKGGILRAHMEVLTDKGQGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLMRGKEVDVRVLKLPFVRNGQKQFD"
     gene            12724..13110
                     /gene="gcvH"
                     /locus_tag="WHT44_000757"
     CDS             12724..13110
                     /gene="gcvH"
                     /locus_tag="WHT44_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ2532972.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDPYGAGW
                     FFKLKPANVADYDSLLTAEQYAGEVD"
     gene            13262..14509
                     /locus_tag="WHT44_000758"
     CDS             13262..14509
                     /locus_tag="WHT44_000758"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ2532973.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPKAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYALDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
BASE COUNT         3540 a         3625 c         4024 g         3423 t
ORIGIN      
        1 accttagaga atcgttctct ttgagctaag gcgaggcaac gctgtactgg tttttgttaa
       61 tccactatat ttcggcgtta acggtcaacc cgtatgccgc ctgcctgttt ttcttcatcc
      121 agtttctttt gcagggtgtc gcaaggtgtg tcgcagtcgc acattttttt catacccaag
      181 gcagtaatgc cgccgcaact gcctttgatg ctgcgtttgg agaaaatata gccgaccgcc
      241 ataccgatga tgacggtcag gaagatgccg aaggtaagga gcagggtttt catggtgttt
      301 cctaatcggt ttgtatgttt agcggagcag tttttcaaat tcggaagaca tggcggtgcg
      361 gtagccgcct ttatccctga caatcaggaa aacagcgagt ttttcgcgct ctgccagctt
      421 taaggcttcg gtttcgccca atacgaataa tcctgtggac aagccgtccg ccgtcatcgc
      481 actgtctgcg accacgctga tggaggcgag gttgtggctg atgggtcgtt tgttgttcgg
      541 gttgatgata tgggagaggc gtttgccgtt tttatcgacg tggaaaatac ggtaatcgcc
      601 ggaagtggca agcgaacggt tgttcagcgg gacgataatc tgcgtattgc cgccttggac
      661 gatattgggc tgctcgatac cgatgcgcca cggttcgccg cgcgcgtttt tgcctttgcc
      721 gtgcaactcg ccgccgattt cgaccagata attttgaatg ccgtattttt ccagttcgcc
      781 cgcaacttta tcaacgccga agcctttggc aatcgaagat aaatccaaat aggccttggg
      841 gtgggttttg ctcaaggaag cgtaatcttt gccttgtttc aaaatgattt tgtctatgcc
      901 cgtataagat gccgcctgtt tgatttgttc cggcgacggt tcacgggtaa cggatttgtc
      961 ggggccgaat ccccaaaggt tgaccaaggg gccgacggtt acgtccagcg cgccgtgtgt
     1021 caggcggttc aggcggacgg cttcggcagt aacgtgtgcg aagtcgcttg aaatgcggag
     1081 gggcttgccg gctgtgtgtt ggttgaaccg gctgatttcg gagtcgggct gataggtgga
     1141 catctgccgg ttgacttctt taagcgcgtc atcgatgcgt ttttgtattt cggcaggtga
     1201 ggggagtttg tcccgattat ttgaaaggta tttgacggta taggtcgtgc ccatcgtttc
     1261 gccttgcagg gtaacggttt gcgcggtttg ttccgaacag gcgttcagga agatgaaacc
     1321 cagggcaaat atcaagacgc ggataaagtt cggcaggcgt gtttcagacg gcatagtgtt
     1381 tgacggtttt ggcaaatggt ttgaattata tcgcaaaacg gccggtatgt ttctatgccg
     1441 atgccgtctg aagggtgttc ggatggcatc ggcatagaaa aaggaagaaa ccgaggtttc
     1501 ttccttttgt atttgaagcc gaatatttaa ccgccgaaat cgtccaagag gatgttttcg
     1561 tcttccacgc ccaagtcttt gagcattttg atgacggact ggttcataat cggagggccg
     1621 cacatataaa attcgcagtc ttccggtgct tcgtggtttt tcaggtggtt ttcgtaaacc
     1681 acgttgtgaa tgaagcccgt gtagccgtcc cagttgtctt ccggcagcgg gtcggacagg
     1741 gcgacgtgcc acgtgaagtt cgggaactct gccgcgagtt ggtcaaagtc ttcgacatag
     1801 aacatctcgc gtttggaacg tgcgccgtac cagaaggtaa tcttacgttt ggagttcaaa
     1861 cgtttcaact ggtcgaaaat gtgggaacgc atcggagcca tacccgcacc gccgccgata
     1921 aataccattt cggcatcggt gtctttggcg aaaaattcgc cgaacgggcc ggaaatcgta
     1981 actttgtcgc cgggtttgag cgaccagatg taggacgaca tttgtcccgg aggcgcatca
     2041 ggtacgcgcg ggggcggcgt ggcgatacgc acgttcagca taatgatgcc tttttcttca
     2101 ggatacgaag ccatagagta ggcacgcaaa atcggctcgt ccactttgga aacgtattgc
     2161 cacaaattgt atttgtccca gtcttcgtga tattccttag gaatgtcgaa gtctttgtag
     2221 gcaacagtgt gaggaggagc ttcaatttga atgtagccgc cggcgcggaa ggggacttct
     2281 tcgccttcgg gaatggcaag cttgagttct ttaatgaacg tggctttgtt atcgttggag
     2341 atgacggtgc attcccattt tttcacgccg aacacttctt cggggacttc gatgtccatg
     2401 tcggttttga cgttgacttg gcacgacaga cggcagcctt cgcgtgcttc gcgtttgctg
     2461 atgtgggaca gctcggtcgg caggatgtcg ccgccgccgc tttttacgac gacgcggcat
     2521 tgtccgcacg aaccgccccc gccgcaggcg gaggggataa agatgccttc gttggcaagc
     2581 gcgcccaaga gtttgccgcc ggcgggcatc gtcagctctt tttcgccgtt gactttgatg
     2641 gtgatgtcgc cttcgctgac cagtttggat ttggcaaaca gaatcatcag tgccaaaacc
     2701 aaaacgatga cggtaaacat cacgatacct aaaataatct ccataccgat ccctttctta
     2761 taactggatg ccagagaacg acataaacgc catcgccatc aggccggcgg cgataaaggt
     2821 aatgcccaag cctttgaggc ctttgggagc gtccgaatat ttcatttttt cggtaatgcc
     2881 cgccaaagcg acaatcgcca acatccagcc caagcccgcg ccgaagccgt atacaacgga
     2941 ctcgccgaag ttgtattcgc gttgcgccat aaacgatacg gcgccgaaaa tcgcgcagtt
     3001 cacggtaatc agcggcaggt agatgcccaa tgcgttatag agggcgggga cgaatttatc
     3061 caagaacatt tccaaaatct gcaccaaagc ggcaatcacg ccgatgaagg tgatgaattt
     3121 caaaaaggtc aaatccacgc cttcggcaat cgcgccgtct ttgagcagcg agtaaacgag
     3181 ttggttgaca gggacggaca gcccgagtac gaaaattacc gccacaccca aaccgaatgc
     3241 ggtggatact tttttggata ccgccaaaaa cgtgcacata cccaaaaaga aggatagtgc
     3301 catattttca atgaagacgg atttgatgaa gaggctcaaa tagtgttcca tagcttattc
     3361 ctccgcctgt tcgggtttcc aggtacgcag tccccaaatc aaaaagccga tgatgaagaa
     3421 cgcgctgggg gcgagcagga acaagccgtt ggtctgatac cagccgccgt cctgcacggt
     3481 ttggaaaacg gtgtagccca agagtttgcc cgagccaatc agttcgcgga cggtggcgac
     3541 gacaagcagc attatcccgt agcccgcgcc gttgccgatg ccgtcgatca ggctttccag
     3601 cggcggctct ttcatcgcaa atgcttcggc gcggcccatc acgatacagt tggtaataat
     3661 cagaccgacg aatacggaaa gctgtttgga caattcgtag gcaaatgcct gcaagagttg
     3721 gtcgaccagc gtaaccagcg acgcgataat cgccatttgc acgataatac ggatgctgtt
     3781 ggggatgtag ttgcgtacca gcgaaatgaa gaagctggaa aaaccggtta ccaaagctac
     3841 ggaaataccc atcacgatgg ccgtctgaag tttggtggta accgccaaag ccgaacaaat
     3901 acccaaaacc tgcaaggcaa tcgggttgtt gtcgataaag ggtgaaaaca tcaaatgttt
     3961 caagcgtttc atatcagcca ttattgcgct cctgctgatt tcaatttgtt caggtagggg
     4021 atatagccgt tttcgccgaa ccagtaggcg aacgaacctt gcacgccttt ggatgtcagc
     4081 gatgcgccgg agagggcatc tacgccgtgt tctttgtccg aacccgcgcc tttgccgacg
     4141 tgcagggcga gtttgccttg tccgtcaaac agttttttgc cgacgaattt ttgctgccac
     4201 aacggattgc cgatttcgcc gcccaagccc ggggtttcgc cttgttcgta gtaggtaatg
     4261 ccgttgatgg tgttgccgtc gggctggatg gcgacaaagc cgtacatgac cgaccacaaa
     4321 ccgttaccgt gcataggcag gatgatttgc ccgattttgc cgtcttcgcc ttttaccaaa
     4381 taaacctcgg tgtatttggc acggcttttg atgcctgcca aatcgtcttc ggttttgatg
     4441 cggatgcttt gggcagggtc tttgcctgcg atgcgcgcgc tgaagtcttt aggcgcatcg
     4501 gcgacgtatt cgccggtcgc caaatcgaca acacgttgct cgatacgctc ggcaaaggtt
     4561 ttaccgatgt cggtgtcctt atccatcaaa ccggctacgc tcaagatata gccttgtttg
     4621 tcttggagtt tttgtttctc ttggatgggt ttcaagccga cgaccgcacc ggcaacgatg
     4681 accgagcaaa tcaggctgac cgccaacacg acaatcagcg tgccgctgaa gctgtcttta
     4741 tcgaatttct tagccattgc tgcgcgcctt tctgcgtttg atgttcgctt gtgcgacgaa
     4801 atagtcgaaa atcggggcaa acaggttggc aaacagaatc gccaacatca tgccttcggg
     4861 gtaagccgga ttgaccacgc ggattaatac gcacatcaca ccgatcagtg cgccgtacca
     4921 ccatttgccg acattggtaa aggaagcgga aacagggtcg gtcgccataa acagcatacc
     4981 gatggcgaag ccgccgacca ccaagtgcca gtaccaaggc atagcaaaca tagcgttggt
     5041 gtccgaaccg atgaagttga acagcgaaga catcgcaatc ataccgatca tcacgccggc
     5101 aataatgcgc caagaagcga tgcgggcaaa cacgataaac gcgccgccga ttaagagtgc
     5161 caaagtggag acttcgccaa tggagccggg cagtttgccg ataaacgcgt ccatccaagt
     5221 gatggtttga ccggttacgg cgtttttcag gccgtctgca ccgtgtgccg cccattgcgc
     5281 cagtgcggtt gcgccggaat agccgtcaac cgccgtccaa accgcatcgc cgctcaagtt
     5341 ggcagggtag gcgaagaaca ggaaagcacg gcctgccagc gcagggttca tgaagttttt
     5401 acctgtaccg ccgaatactt ctttcgcaac cacaacgccg aaagaaatac ccaaagccgc
     5461 ctgccacagc ggcagcgtgg gcggaacgat taaggcaaac agaatcgaag taacgaagaa
     5521 accttcgttg atttcgtgtt tgcgcacggt ggcgaacaaa acttcccaga aaccgcccac
     5581 aacaaataca gtcgcgtaaa tcggcaggaa gtaaatcgcg ccaaacagca ttttgtccga
     5641 cacgcccgct tcagacgaca tattgatgcc caaagcgttg gcaaaggcgt aatgccagtc
     5701 gttggcgatg ttttgttgca gcaaatcagg cgttaacgca ccgaatgcct gcgcgccgac
     5761 gttgtacata ccgtagaaca tggcagggaa caaagccagc cacaccaaaa tcatcatgcg
     5821 cttggagtcg agcgcgtcgc ggacgtgcgc cgctttgcgc gttaccgcgc cggatgtgta
     5881 gaaaattgtc gccgcagctt cgtagagggc ataccatttt tcatgtttgc cgcccggcag
     5941 gaagtgcggt tcgatttttt ccagaaaatg tttcaagccc ataatcagcc ttccttctca
     6001 atggtttcca gcactttgcg caacagcggg ccgtattcgt atttgcccgg gcagacgaag
     6061 ctgcacaaag cgaggtcttc ttcgtccaat tccaagcaac ccaatgcctg cgcgctgtcg
     6121 gtatcgccga cgattaaatc gcgcaaaagc agggtgggca ggatatccaa gggcatcacg
     6181 cgctcgtaag taccaatcgg caccatggcg cggtcgccgc cgttgacggc tgtgttgaac
     6241 ttgaagagtt tgtttttcag gaaatggccg agggttgtac gcgtgatgga gtatttgtcc
     6301 ggctgcggcg caacccagcc gaacagctct ttgctgcggc cttcttcgat aacggaaatc
     6361 tgattgtggt agcgtcccaa ataatcgtgc gcgccttgtg taatcgcgcc gttcaatacc
     6421 gaaccggaaa tcacgcggtt gtctgtgtca accaattcgc ccgcagtaat ttgcgatact
     6481 ttcgcaccca aaacggtacg caagaggcgc ggtttgttga cttgagaacc acctagggca
     6541 atcacgcgct cggtgttcag acggcctgtt gcaaacaaac ggccaatggt aattacatct
     6601 tgataattga tggtccacac ggttttattc gcgccgaccg gctcgatgaa atgaatgtgc
     6661 gtgccactca aaccggcagg atgcgggccg ccgaattcat gtgtttcgat gttggcagca
     6721 ttttcagacg gcacgtctgc gccagctgcc ttacaaacat ggattttgcg ttcggtcaaa
     6781 cggctcaata ccaacaggcc gcgtttgaaa tcctcggcgg cttctttgat aatgaccgta
     6841 gggtcggcag ccagcggatt ggtgtccatc gcattgacga agatggcgaa cggctcggca
     6901 tcgacggcag gaattttgct gaacggacgg gtgcgcagcg cagtccacaa accggattgg
     6961 atcaggttgc ggcgcacttc ttcgccgctt aagtttgcca gcgcttcagg tgcgtagcgt
     7021 tcaaactcga tttcgtcgtt gccttcaacg gcaatcacga ctgactgaag tacgcgcttt
     7081 tcgccacggt gaatcgcggc gattttgcct gaagccggcg cagtaaacac cacgcccgga
     7141 ttctttttgt cttcaaacag cacttggcct tttttgacgg catcgccttc cttgactttc
     7201 atcgaggggc gcataccggc atattcttcg ccaagcaacg cgacttcggt aatggccggg
     7261 ccgtcgtaaa cggcttgctc cggtctgccc gcgatgggca ggtttagacc ttttttgatt
     7321 ttaatcatat atttgcatta cttgtgatgg ttaaggtaaa aacggcgtgt tttgataccg
     7381 tgtcgcgtgg catcaaaagc attgaataaa ttaatgtagc aaagtgttag attctatcag
     7441 gaattgtacc tgtttgtcag atttgctgct tttttccttg cggaagccgt ttttatagtg
     7501 gattaaattt aaaccagtac ggcgttgcct cgcccttgcc gtactatttg tactgtctgc
     7561 ggcttcgtcg ccttgtcctg atttttgtta atccactata aattgtcgga aggggggata
     7621 ttgatttgat tatgccggaa tttaaaatgc cgtctgaatg ttcagacggc atagcgttta
     7681 cagcagtttg aaaacgaaaa agataagggt atgtacgatg aagacgggtg tcaggaaggc
     7741 gaccgaccac atcatatagc cgaagaaagt cggcatcggt acgccgcgct gttcggcaat
     7801 ggccttgacc atgaagttcg gtgcgttgcc gatgtaggtc agtgcgccca tgaataccga
     7861 acccatagaa accgccagca gcgaatgaaa cagggtaccc gtcatcaagg cttgggcatc
     7921 gccgcccgcc atattgaaaa aaacgagata agtgggcgcg ttatccaaga atgccgacaa
     7981 tatgccgctc atccaaaaat acatcacatt aatcggatga cctgccgtat cgtgaaccag
     8041 cgataccacc ccgcccagcg cgcctgcctc gcctgctttc agaatgctca ggacgggaaa
     8101 gatggtgatg aagatgccga ggaagagttt gcccacttcg gcgatgggtt caaagttgaa
     8161 ttcgttgcct gcgcggactt gtttgggcgt gattgccata gatacggcgg tcaatgcaat
     8221 caggatgaca tcgcggacga ggttttgcag ggcgtaacgg ctgccgagga tttcaaatcc
     8281 cgggtgttcg ggtttccaaa ggccggacat tagaaccgcg ccgaccacgc ccgaaagcag
     8341 gaggaagttc catttgccga agatggcgat tttttcgggt ttttcctgtt gtgccggcgt
     8401 atcttgtgca atgctttcct gtttgaagaa acggttgtcg atgaaataga aggcggtcaa
     8461 caggacagcg gtgctgatca ggacgggggc gaacatatgt ttgaccgtcc acatgaaatc
     8521 tacgcctttg aggaagccga ggaagagtgg ggggtcgccc aaaggggtca gaccgccgcc
     8581 gatgtttgca accaggaaaa tgaagaagat gacgatgtgc acgcggcggg tacggttttg
     8641 gttggctttc agcagcggac gaatcatcag cattgctgcg ccggtcgttc ccatgataga
     8701 ggcaagtgcc gtaccgacgg caagcagggc ggtgttgagc ttgggtgtgc cgttcaagtc
     8761 gccccaaacc aaaatgccgc ctgaaatggt gtacagggca agcagcagca ggatgaaagg
     8821 gatgtattct tcaacgagtg cgtgtgcgac ggtatggata ccggcggacg cgccaaaaac
     8881 caaactgaac gggatgagga agagcaatgt ccaaaaggcg gtaattttgc cgtaatggtg
     8941 atgccaggta tgcgaaaaaa acaagggacc caatgcgata gacagcaaaa tcagggcaaa
     9001 gggcaggccc cacagcaggt ttaggtttgc gccgtccaaa tctgcggcgt aaaccgatgc
     9061 tgggaaaagc attagtgaaa acaggggtag gtggcgcatc gtgtttcctc gattcaagca
     9121 ctgccttgcg cggcgcgtgg gagtgataca ggcaccgtgc cgcccggaca taggcggact
     9181 gtgctgcctg tttgtttttg aaaaaagttt tccgattgat tgtaaagtaa ataatcagga
     9241 taaaccagtt tcccaaaccg gaaggcggcg ggaaggcgga ttgctgtgct tgggaatata
     9301 tgttcttttt gataaataat tttatttaaa acaaataaat atatgtcttt aataaataat
     9361 ctatcgaaaa cgaaaaatga atttatttta acatatattt gcaatgaaac aggtttgccc
     9421 cccccccgtt tgtttgccct tatccctttc agtacggcat tcaagattcg ggcctgcgcc
     9481 acatccatat ggcgacaagg gaacaaaaaa ccgatgaaac cgccccgacc caccagcgtt
     9541 ggggaaactg ccaaaacatt atcaggcagg atgcggtcat catactgatg gcgaatattt
     9601 tggctttgcg cggcactgcg ccgttttgtt cccagttatg aaccatcggg ccgaaatagc
     9661 ggtgccggtg cagccagcgg taaaagcgcg gggatgcctt tgcccagcag gcggcggaga
     9721 gcagtacgaa cggcgtggtc ggcaacagcg gcaaaaaaat gccgatgata cccaacagta
     9781 gggaaatgca gccgcaggca attaaaagat aacgtatcat tttgaaatat ttttcttatt
     9841 gtgcggataa gggcaggatg tgataccgag ttttgcccag ccttcatgtc ccattttttc
     9901 cagcagggcg atattgcgtt cgaatattgc cgaagcgtcg ggaaaggctt gtgcggcttt
     9961 ggcaatgctg tcttcgcgga tgaggtgcag cgtcggatag ggagaacggt tggtgtagtt
    10021 gccaatgtcg tctgaatccg tgccttcaaa ttggaaatcg ggatgaaacg gggcgatttg
    10081 gacgatgcct tctaagccgt tttcgacaac ggcggcatcg gcaatgtcga gcatatcgtt
    10141 gaatacgtcg aaatcgggga atagggtcgg gtgaaccagc agggtggttt ccagttcggt
    10201 ggcgggtgta ttgcccagtc gctgcagttc ttcgtccaag tcttccaaaa aaccgtcaag
    10261 gtgtttggct tcgctgatcg cgatgcggac aaggttttta acgtgggggg ctttggcaaa
    10321 gggacacagg ttcagaccga tgacggcttt ttccaaccat tgtccggtgt gttcggcaac
    10381 agcatcttta ttttcggaag tattgatatt cattattgtc atgtaaatgt gtttgcagat
    10441 tgcacgtgcg ggaaaatcgg gaagggcact attccttcag caggtggttg agcggcaggg
    10501 aggtggtgtg tttgatttct tttaaaacaa agctcgattg cgcatcttgt acgccgtggt
    10561 gggacaggag cgtatccaaa acaaaatggg aaaacgcgtt catatcggta aaaaacgcct
    10621 gaagcaggta gtcggtttcc cctgtcaggg cgaagcagct caagacttca ggccattttc
    10681 gaaccgatgc ggcaaagtct tcccgcgcgt cttttgcttt gcggatggaa acgcggataa
    10741 atgcctgaag tcccaagttg acagattccg gagacagcag cgcggcatat tggcggacga
    10801 taccggcatc ttccaactgc ttcagacggc gcaggcacgg agaaggcgaa agtgcgacac
    10861 gttcggacag ttcgacattg gtcagcctgc cgttttcctg gagaacctgt aagattttaa
    10921 tatcggtttt gtctaaagtg agttggggca tatttgcgtt ccgttttaag gaattcggat
    10981 tgtctgtccg tatgtttgcg gcaatccgca cagatggaga ccatattaac atataaaaag
    11041 ttataccgtc atccgggaca aattttgttt tcggaaaatc atgtgaaaac agaggcggtc
    11101 ggtttgcatc tctttaagac ggcttgccca aaccgccgat tcaagacata atcgggaaat
    11161 gtgcaggaga gtgttacacc caactacaat gtaaccaccg aaggcgcaga cacccttaaa
    11221 tcgctcaggt atcagggact gcacattgaa acaaacaatc tggagagcgg cgttggaata
    11281 acgtccaccg aaggggagaa ggccgtctga accaccattc agacaaccgc gcaaagcagt
    11341 gagcagactg gtttgccatc atgcggatac agccgaaaat ctcaggttca aggacagata
    11401 gggtcatccg cgcacaggtg cgcgggcggc atctgaacaa aaaatccgga gaaacttgag
    11461 aatgactgct ctgaaaacca ccccatttca tcaagcccat caagatgcag gcgcgaagct
    11521 ggtcgatttt gccggctggg agctgcccat ccattatggt tcacaaatcg ccgaacacga
    11581 agccgtgcgc accgacgccg gtatgtttga cgtatcccat atgctcgtta ccgacgtagc
    11641 aggcgcaaat gccaaagcct ttttccgcaa attgattgcc aacgatgtcg ccaagctcgc
    11701 ttttgtcggc aaagcccttt attccgcttt gctcaacgac aacggcggtg tgattgacga
    11761 cttaatcgtt taccgcacca atgaagccga aacccaatac cgcatcgtgt ccaacggcgc
    11821 gacccgcgaa aaagacacgg cgcaattcca caaagtcgga caagagttcg gcgtcgcctt
    11881 caatccgcgc tacgacctcg gcatgctcgc cgtacaaggc cctaaagcca ttgaaaaact
    11941 cctgaccgtc aaacccgaat gggcagatgt cgtccataac ctcaaaccgt tccaaggcgc
    12001 ggatttgggc aacgactggt ttgtcgcccg caccggctac accggcgaag acggcgtcga
    12061 agtcatcctg cccggcaccg aagccgtcgc attcttcaaa gccctgcaac aagccggcgt
    12121 acagccctgc ggcctcggcg cgcgcgacac cctgcgcatg gaagccggca tgaacctcta
    12181 cggcaacgat atggacgacg acaccagccc gctcgaagca ggtatgggtt ggaccgttga
    12241 cttgaaagac gaaagccgcg acttcgtcgg caaagccgcc ttgctggcat tgaaagaaaa
    12301 aggcgttgcc gtcaaacagg tcggcctgtt gctcgaaaaa ggcggcatcc tgcgcgcgca
    12361 tatggaagtg ttgaccgaca aaggccaagg cgaaaccacc agcggcgtat tctccccaag
    12421 cctgaaacaa tccatcgcca tcgcgcgcgt accgaaagat tttgacggcg ataccgccaa
    12481 agtgctgatg cgtggcaaag aagtggacgt gcgtgtactg aagctgccgt ttgtccgcaa
    12541 cggacagaaa cagtttgatt gatgcggttt cagacggcat tttcatttca tatgccgtct
    12601 gaaagcaggt tttaattgtt gtccgatacg gacgtttgta gaaagcattg aacaaggcat
    12661 ctgtggatat tgattcatgc agatgccgtc tgaaaataac ccctatcaat ggagtatcaa
    12721 accatgagca acaacatccc ggccgaactg aaatacgttg ccagccatga atggctgcgc
    12781 cttgaagaag acggtacgat taccgtcggc attacccacc acgcgcaaga gctgttgggc
    12841 gacatcgtgt tcgtcgagct gcccgaagtc ggcgcgaacc ttgccgctga agagcaagcc
    12901 ggtgtggttg agtctgtaaa agccgcgtcc gacgtgtacg caccgattgc aggcgaagtc
    12961 gttgccgtca acgaagattt gccaagcgct ccggaaactg ccaacagcga tccttacggt
    13021 gcaggctggt tcttcaaact caaaccggca aacgttgccg attacgacag tctgctgact
    13081 gccgaacaat acgcgggcga agtggattaa accgcccggc tgcccgacgg caaccgccgg
    13141 acaaacggaa actgcacctt cagacggcat ttttgcggtc ggaggtgcag ttttttgtcc
    13201 gtgttttaag gaagcagtta ggctataata acggtctata ttcatcttta ccgatttttt
    13261 catgcaactt accgctgtcg gactcaatca tcaaaccgca cctttaagca tacgggaaaa
    13321 gctggcgttt gccgccgccg ccctgcctaa agccgtccgc aatcttgccc gaagcaatgc
    13381 ggcaacggag gcggtaatcc tttctacctg caaccgcacc gagctttact gcgtcggtga
    13441 ttcggaagaa atcatccgat ggcttgccga ttaccacagt ttgccgattg aagaaatccg
    13501 tccgtatctg tacgcgctgg atatgcagga gactgtgcgc catgctttcc gcgtcgcctg
    13561 cgggctggat tcgatggtgt tgggcgagcc gcagatttta ggacagatta aggatgccgt
    13621 tagggttgct caagagcagg aaagtatggg taagaaactc aatgccctgt tccaaaaaac
    13681 cttttccgtt gctaaagagg tccgtaccga tactgccgtc ggcgaaaact cggtttccat
    13741 ggcttccgct tccgtcaaat tggcggaaca gatttttccc gacatcggcg atttgaatgt
    13801 cttgtttatc ggcgcaggcg aaatgattga gctggttgcc acttattttg ccgccaaaag
    13861 tccccggctg atgacggttg ccaaccggac gctggcgcgt gcacaggagt tgtgcgacaa
    13921 gctcggtgtc aacgccgaac cgtgcctgct gtccgatctg cctgccattc tgcacgatta
    13981 cgacgtagtg gtttcttcaa cggcaagcca gttgcccatt gtcggcaaag gcatggtgga
    14041 gcgtgcattg aaacaaaggc agagtatgcc gttgttcatg cttgatttgg cagtgccgcg
    14101 tgacattgaa gcggaagtcg gcgatttgaa tgatgcctat ctttatacgg tggacgatat
    14161 ggtcaatatc gtccaaagcg gcaaggaggc aaggcagaag gccgccgccg ccgccgaaac
    14221 gctggtgtcc gagaaagttg ccgaatttgt caggcagcag cagggcaggc agagtgtccc
    14281 cttgattaag gcgttgcggg acgagggcga gaaagcgcgc aaacaggtgt tggaaaatgc
    14341 catgaaacag cttgccaaag gcgcaacggc agaagaggtt ttggaacggc tgtccgtcca
    14401 actgaccaac aagctgctgc attcgccgac ccaaaccttg aataaggcgg gggaagaaga
    14461 taaagatttg gttcatgccg tcgcgcagat ttatcatttg gacaaataac ggtgcgccgg
    14521 gaaaaatgcc gtctgaagag gtttcagacg gcattttttt gtgccgcctg acaacatcgt
    14581 gaaatcccac attatatcga tgtaatcaca aa
//
LOCUS       DAWGRZ010000036        14462 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000036 DAWGRZ010000000
VERSION     DAWGRZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14462
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.036"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..412)
                     /locus_tag="WHT44_000759"
     CDS             complement(<1..412)
                     /locus_tag="WHT44_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532974.1"
                     /translation="MNRTLYKVVFNKHRNCMIAVAENAKREGKNTADTQAVGILPNDI
                     AGFAGFIHSISVISFSLSLLLGSALILTSSSATAQGIVADKSAPAQQQPTILQTGNGI
                     PQVNIQTPTSAGVSVNQYAQFDVGNRGAILNNSRS"
     gene            505..603
                     /locus_tag="WHT44_000760"
                     /pseudo
     CDS             505..603
                     /locus_tag="WHT44_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(609..1025)
                     /locus_tag="WHT44_000761"
                     /pseudo
     CDS             complement(609..1025)
                     /locus_tag="WHT44_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(1122..2105)
                     /locus_tag="WHT44_000762"
     CDS             complement(1122..2105)
                     /locus_tag="WHT44_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEZ2532975.1"
                     /translation="MLLTLHGTDKFCIVNNFHTNRNTAYKALKDACNNLFERQFSFIE
                     KTPKGEKVVRTRWVSQVAYIEQQATVELVFAPNVAPLITMLEKNFTSYELDQVSSLSS
                     KYAVRLYEIIISWRAAGKTPMFSTMELRERLGMMPDEYQKMELFKRKVLDFAVKQIND
                     KTDISITYEQHKEGRKIVGFTFSILHKIGSKDIPLENQSELFAGMTDLEAGTIRVRAE
                     AYIASLIAKGQNVTKAHRLNILKKAVEERWGFEDVAKDNGAKNPENKNAKVVLNEWEK
                     ISNGTRFKDKDGTIWVKDSGMLRTEGTNRWIADSQIAKLFPMLTVMVEEGI"
     gene            2052..3731
                     /gene="hrpB"
                     /locus_tag="WHT44_000763"
     CDS             2052..3731
                     /gene="hrpB"
                     /locus_tag="WHT44_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ2532976.1"
                     /translation="MEIINDAELIRSMQRQQHIDAELLTDANVRFEQPLEKNNYVLSE
                     DETPCTRVNYISLDDKTVRKFSFLPSVLMKETAFKTGMCLGSNNLSRLQKAAQQILIV
                     RGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEKRDGKSAEGSISAFNNKFPLYR
                     NKILNLRDVEQGLENLRRLPSVKTDIQIIPSEEEGKSDLQIKWQQNKPIRFSIGIDDA
                     GGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHKTDLTDATGTETESGSRSYSVH
                     YSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQYQSSLAAERMLWRNRLHKTSVG
                     MKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYLNRWQLDGKLSYKRGTGMRQSM
                     PAPEENGGDILPGTSRMKIITAGLDAAAPFILGKQQFFYATAIQAQWNKTPLVAQDKL
                     SIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNHQFYLGADYGRVSGESAQYVSG
                     KQLMGAVVGFRGGHKVGGMFAYDLFAGKPLHKPKGFQTTNTVYGFNLNYSF"
     gene            3840..9827
                     /gene="hrpA"
                     /locus_tag="WHT44_000764"
     CDS             3840..9827
                     /gene="hrpA"
                     /locus_tag="WHT44_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion exoprotein HrpA"
                     /protein_id="HEZ2532977.1"
                     /translation="MNKGLHRIIFSKKHSTMVAVAETANSQGKGKQAGSSVSVSLKTS
                     GDLCGKLKTTLKTLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQ
                     TPNGRGLSHNRYTQFDVDNKGAVLNNDRNNNPFVVKGSAQLILNEVRGTASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGA
                     AGWNDKGGADYTGVLARAVALQGKLQGKNLAVSTGPQKVDYASGEISAGTAAGTKPTI
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADG
                     TEASPTYLSIETTEKGAAGTFISNGGRIESKGLLVIETGEDISLRNGAVVQNNGSRPA
                     TTVLNAGHNLVIESKTNVNNAKGPATLSADGRTVIKEASIQTGTTVYSSSKGNAELGN
                     NTRITGADVTVLSNGTISSSAVIDAKDTAHIEAGKPLSLEASTVTSDIRLNGGSIKGG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGSLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTAL
                     QGNIVSDGLHAVSADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNIT
                     SSSGDITLVAGNGIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSD
                     RALSIENTKLESTHNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLV
                     ANGVLALNARYSQIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPL
                     AGRLNIEAGSGTLTIEPANRISAHTDLSIKTGGKLLLSAKGGNAGAPSAQVSSLEAKG
                     NIRLVTGETDLRGSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKE
                     LEQQIAQLKKSSPKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQN
                     IDLISAQGIEISGSDITASKKLNLHAAGVLPKAADSEAAAILIDGITDQYEIGKPTYK
                     SHYDKAALNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLE
                     AGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTT
                     RFNAPAGKVTLVAGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNYSKNELNETKL
                     PVRVVAQTAATRSGWDTVLEGTEFKTTLAGADIQAGVGEKARADAKIILKGIVNRIQS
                     EEKLETNSTVWQKQAGRGSTIETLKLPSFESPTPPKLTAPGGYIVDIPKGNLKTEIEK
                     LAKQPEYAYLKQLQVAKNVNWNQVQLAYDKWDYKQEGLTRAGAAIVTIIVTALTYGYG
                     ATAAGGVAASGSSTAAAAGTAATTTAAATTVSTATAMQTAALASLYSQAAVSIINNKG
                     DVGKALKDLGTSDTVKQIVTSALTAGALNQMGADIAQLNSKVRTELFSSTGNQTIANL
                     GGRLATNLSNAGISAGINTAVNGGSLKDNLGNAALGALVNSFQGEAASKIKTTFSDDY
                     VAKQFAHALAGCVSGLVQGKCKDGAIGAAVGEIVADSMLGGRNPATLSDAEKHKVISY
                     SKIIAGSVAALNGGDVNTAANAAEVAVVNNALNFDSTPTNAKKHQPQKPDKTALEKII
                     QGIMPAHAAGAMTNPQDKDAAIWISNIRNGITGPIVITSYGVYAAGWTAPLIGTAGKL
                     AISTCMANPSGCTVMVTQAAEAGAGIATGAVTVGNAWEAPVGALSKAKAAKQAAPKET
                     INNLANLAKAEQQILFRIAQRDTQLDAWKTGFNNRVRKGAGLLDASNIPITINGKTIK
                     PVQAISLKGAPVYSGVSEQEIFALYRQMTGQNPNFRVLPDGRLANGIISTGEWAGTKI
                     ALRNFSKTENSTQARWTLDLQNPPSFIKGTKLELKFQ"
     gene            9846..10220
                     /locus_tag="WHT44_000765"
     CDS             9846..10220
                     /locus_tag="WHT44_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ2532978.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDENLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
     gene            10579..12891
                     /locus_tag="WHT44_000766"
     CDS             10579..12891
                     /locus_tag="WHT44_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ2532979.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRILLSTTDISSENGK
                     IKLQSYGDQFYYAGQGELYTFDKRSYKTGKWYKLKHVTEIKEHKNAKADPVSLSASQG
                     IEIKSGGNIGAHATLFDAPRGSVKIEAGRGLVLYAVEDLNYDKLDTRTKRKFIGITYD
                     KVHDTTTHTMKTALPSRVVAESANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARAD
                     AKIILEGIKSSVRTETVSSSKSALWQKQAGRGSNIETLQLPSFTGSVAPVLSAPGGYI
                     VDIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNVNWNQVQLAYDKWDYKQEGLTRAGAA
                     IIALAVTVVTAGAGVGAALGLNGAAAAAADAAFASLASQASVSLINNKGDVGKTLKEL
                     GRSRTVKNLVVAAATAGVSNKLGASSLATWSETPWVNNLNVNLANAGSAALINTAVNG
                     GSLKDNLEANILAALVNTAHGEAASKIKQLDQHYIAHKVAHAVAGCAAATANKGKCQD
                     GAIGAAVGEIVGEALVKNTDFSDMTPEQLDLEVKKITAYAKLAAGTVAGVTGGDVNTA
                     AQTAQNAVENNAVKAVVTAAKVVYKVARKGLKNGKINVRDLKQTLKDEGYNLADNLTT
                     LFDETLDWNDAKAVIDIVVGTELNRANKGEAAQKVKEVLEKNRPYIPNKGAVPNMSTY
                     MKNNPFGKQLAQISEKTTLPTQQGQSVFLVKRNQGLLKTGDRFYLDGQHKNHLEVFDK
                     NGNFKFVLNMDGSLNQMKTGAAKGRKLNLK"
     gene            12900..13277
                     /locus_tag="WHT44_000767"
     CDS             12900..13277
                     /locus_tag="WHT44_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532980.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            13555..13929
                     /locus_tag="WHT44_000768"
     CDS             13555..13929
                     /locus_tag="WHT44_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532981.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDELEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
     gene            13964..>14462
                     /locus_tag="WHT44_000769"
     CDS             13964..>14462
                     /locus_tag="WHT44_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ2532982.1"
                     /translation="MRKVCASARTRSMIYGSMPSEKLTIFQTAFVMQVNIQIPYILPR
                     CVRAEDTPYACYLKQLQVTKDVNWNQVQLAYDKWDYKQEGLTGAGAAIIALAVTVVTA
                     GAGAGAALGLNGAAAAATDAAFASLASQASVSLINNKGNIGNTLKELGRSSTVKNLMV
                     AVATAG"
BASE COUNT         4299 a         3354 c         3385 g         3424 t
ORIGIN      
        1 tgctgcgact gttgtttaaa atcgccccgc gattacccac atcaaactgg gcgtattgat
       61 taacagaaac ccctgccgaa gtaggggttt gaatattgac ttgcggtatg ccgttacctg
      121 tttgcaggat ggtaggctgt tgctgtgcag gtgcggattt gtcggcaacg ataccttggg
      181 cagtagcaga agaagaagtc aggataaggg cagaaccgag cagtaatgaa agggagaatg
      241 agataacaga gatagaatgg ataaaacccg caaagcccgc aatatcattt ggcaaaatac
      301 ctacagcttg ggtgtcggct gtgtttttgc cctcgcgttt ggcattttca gcaacggcta
      361 tcatgcagtt tcgatgtttg ttaaatacaa ctttgtacag ggtgcggttc atagtaaggg
      421 ctttcttaat aatattttta taatcgtaaa ttagattaat ttttaggggc tgacgtagat
      481 taacagttat gccaggctac gaaaataaag ataaccaatt gtaaattaaa caatagagtt
      541 caaaagaaac tgcttgaatt tttcgtactc caagctaccg cccgttccgc tgccgatatt
      601 ttgggtatgg cgctgcgggc aatttccgtt cccacttcgg cgagttggcg cataatggaa
      661 cgctcgcgca cgatttcggc atggcgccgg atgttggcgg cagacggagt attttgcgcc
      721 agcgtaatca gatattcgaa tccccccgcc gcttccagct cttcgttccg ctgcaaatct
      781 tcctgaaccg tgatgacatc ggcaggacgg ctctcattga tcagtttggc aatggatcgg
      841 aaaatcaggc ggtgttcgtg gcggtagaaa tcctctcccg aaaccacatc ggcaatcctg
      901 tcccaagccg gattttccag catcaacccg cccaaaacgg attgttccgc ctccattgag
      961 tgcggcggaa gcgataatga gccgattcct ccgtcttcag acggcatggc tgtgtaatcg
     1021 ttcatggtac atccgacaaa attgcaatct tctattgtag cgtaaagcag gttcaattgg
     1081 tttccgtacc gcaaaacagg tagaatacgc gagttgccgg gttaaatacc ttcctcaacc
     1141 atcacagtta acataggaaa taatttggca atctgagaat cggctatcca cctgtttgtc
     1201 ccttcagtcc taagcatacc tgaatcttta acccaaattg ttccatcctt gtccttaaaa
     1261 cgtgtgccat tagaaatctt ttcccattcg tttaaaacga cttttgcatt tttgttttca
     1321 ggatttttgg ccccattatc tttagccaca tcttcaaatc cccaacgttc ctctacggct
     1381 tttttcagaa tattcagcct atgggcttta gtcacgttct gaccttttgc aatgagcgaa
     1441 gcgatatatg cttccgccct gacccgtatc gttccggctt ccaaatcagt cattccggca
     1501 aaaagttccg attgattttc aagagggatg tctttcgacc ctattttatg taggattgag
     1561 aatgtaaaac ctacaatttt tcgtccttct ttatgctgct cgtaggtaat ggaaatatcc
     1621 gttttatcat tgatctgctt gacggcgaaa tccaaaacct tacgtttgaa tagctccatt
     1681 ttttgatact cgtcaggcat catacccaaa cgttcgcgca actccattgt actgaacatc
     1741 ggtgtcttac cggctgcacg ccatgaaata ataatttcgt agagccgcac cgcgtattta
     1801 ctgctcaacg atgagacctg atcaagctcg tagcttgtga agtttttttc tagcatcgta
     1861 atcaaagggg caacatttgg tgcaaaaact aactctaccg ttgcctgttg ttcaatatag
     1921 gcgacttgag atacccacct tgtccgtact accttttccc cttttggtgt tttttcgata
     1981 aaactgaatt ggcgttcaaa aaggttgtta caggcatctt tcaaagcctt atacgccgta
     2041 ttacggttgg tatggaaatt attaacgatg cagaacttat ccgttccatg cagcgtcagc
     2101 agcacataga tgctgaattg ttaactgatg caaatgtccg tttcgagcaa ccattggaga
     2161 agaacaatta tgtcctgagt gaagatgaaa caccgtgtac tcgggtaaat tacattagtt
     2221 tagatgataa gacggtgcgc aaattttctt ttcttccttc tgtgctcatg aaagaaacag
     2281 cttttaaaac tgggatgtgt ttaggttcca ataatttgag caggctacaa aaagccgcgc
     2341 aacagatact gatcgtgcgt ggctacctca cttcccaagc tattatccaa ccacagaata
     2401 tggattcggg aattctgaaa ttacgggtat cagcaggcga aataggggat atccgctatg
     2461 aagaaaaacg ggatgggaag tctgccgagg gcagtattag tgcattcaat aacaaatttc
     2521 ccttatatag gaacaaaatt ctcaatcttc gcgatgtaga gcagggcttg gaaaacctgc
     2581 gtcgtttgcc gagtgttaaa acagatattc agattatacc gtccgaagaa gaaggcaaaa
     2641 gcgatttaca gatcaaatgg cagcagaata aacccatacg gttcagtatc ggtatagatg
     2701 atgcgggcgg caaaacgacc ggcaaatatc aaggaaatgt cgctttatcg ttcgataacc
     2761 ctttgggctt aagcgatttg ttttatgttt catatggacg cggtttggcg cacaaaacgg
     2821 acttgactga tgccaccggt acggaaactg aaagcggatc cagaagttac agcgtgcatt
     2881 attcggtgcc cgtaaaaaaa tggctgtttt cttttaatca caatggacat cgttaccacg
     2941 aagcaaccga aggctattcc gtcaattacg attacaacgg caaacaatat cagagcagcc
     3001 tggccgccga gcgcatgctt tggcgtaaca gacttcataa aacttcagtc ggaatgaaat
     3061 tatggacacg ccaaacctat aaatacatcg acgatgccga aatcgaagta caacgccgcc
     3121 gctctgcagg ctgggaagcc gaattgcgcc accgtgctta cctcaaccgt tggcagcttg
     3181 acggcaagtt gtcttacaaa cgcgggaccg gcatgcgcca aagtatgcct gcaccggaag
     3241 aaaacggcgg cgatattctt ccaggtacat ctcgtatgaa aatcattact gccggtttgg
     3301 acgcagccgc cccatttatt ttaggcaaac agcagttttt ctacgcaacc gccattcaag
     3361 ctcaatggaa caaaacgccg ttggttgccc aagataaatt gtcaatcggc agccgctaca
     3421 ccgttcgcgg atttgatggg gagcagagtc ttttcggaga gcgaggtttc tactggcaga
     3481 atactttaac ttggtatttt catccgaacc atcagttcta tctcggtgcg gactatggcc
     3541 gcgtatctgg cgaaagtgca caatatgtat cgggcaagca gctgatgggt gcagtggtcg
     3601 gcttcagagg agggcataaa gtaggcggta tgtttgctta tgatctgttt gccggcaagc
     3661 cgcttcataa acccaaaggc tttcagacga ccaacaccgt ttacggcttc aacttgaatt
     3721 acagtttcta acctctgaat tttttactga tatttagacg gtctttcctt atcctcagac
     3781 cgtcaaactt tacctacgta cttggcgcgc agtacgttca tcttcaaaat ggaatagaca
     3841 tgaataaagg tttacatcgc attatcttta gtaaaaagca cagcaccatg gttgcagtag
     3901 ccgaaactgc caacagccag ggcaaaggta aacaggcagg cagttcggtt tctgtttcac
     3961 tgaaaacttc aggcgacctt tgcggcaaac tcaaaaccac ccttaaaact ttggtctgct
     4021 ctttggtttc cctgagtatg gtattgcctg cccatgccca aattaccacc gacaaatcag
     4081 cacctaaaaa ccagcaggtc gttatcctta aaaccaacac tggtgccccc ttggtgaata
     4141 tccaaactcc gaatggacgc ggattgagcc acaaccgcta tacgcagttt gatgttgaca
     4201 acaaaggggc agtgttaaac aacgaccgta acaataatcc gtttgtggtc aaaggcagtg
     4261 cgcaattgat tttgaacgag gtacgcggta cggctagcaa actcaacggc atcgttaccg
     4321 taggcggtca aaaggccgac gtgattattg ccaaccccaa cggcattacc gttaatggcg
     4381 gcggctttaa aaatgtcggt cggggcatct taactaccgg tgcgccccaa atcggcaaag
     4441 acggtgcact gacaggattt gatgtgcgtc aaggcacatt gaccgtagga gcagcaggtt
     4501 ggaatgataa aggcggagcc gactacaccg gggtacttgc tcgtgcagtt gctttgcagg
     4561 ggaaattaca gggtaaaaac ctggcggttt ctaccggtcc tcagaaagta gattacgcca
     4621 gcggcgaaat cagtgcaggt acggcagcgg gtacgaaacc gactattgcc cttgatactg
     4681 ccgcactggg cggtatgtac gccgacagca tcacactgat tgccaatgaa aaaggcgtag
     4741 gcgtcaaaaa tgccggcaca ctcgaagcgg ccaagcaatt gattgtgact tcgtcaggcc
     4801 gcattgaaaa cagcggccgc atcgccacca ctgccgacgg caccgaagct tcaccgactt
     4861 atctctccat cgaaaccacc gaaaaaggag cggcaggcac atttatctcc aatggtggtc
     4921 ggatcgagag caaaggctta ttggttattg agacgggaga agatatcagc ttgcgtaacg
     4981 gagccgtggt gcagaataac ggcagtcgcc cagctaccac ggtattaaat gctggtcata
     5041 atttggtgat tgagagcaaa actaatgtga acaatgccaa aggcccggct actctgtcgg
     5101 ccgacggccg taccgtcatc aaggaggcca gtattcagac tggcactacc gtatacagtt
     5161 ccagcaaagg caacgccgaa ttaggcaata acacacgcat taccggggca gatgttaccg
     5221 tattatccaa cggcaccatc agcagttccg ccgtaataga tgccaaagac accgcacaca
     5281 tcgaagcagg caaaccgctt tctttggaag cttcaacagt tacctccgat atccgcttaa
     5341 acggaggcag tatcaagggc ggcaagcagc ttgctttact ggcagacgat aacattactg
     5401 ccaaaactac caatctgaat actcccggca atctgtatgt tcatacaggt aaagatctga
     5461 atttgaatgt tgataaagat ttgtctgccg ccagcatcca tttgaaatcg gataacgctg
     5521 cccatattac cggcaccagt aaaaccctca ctgcctcaaa agacatgggt gtggaggcag
     5581 gctcgctgaa tgttaccaat accaatctgc gtaccaactc gggtaatctg cacattcagg
     5641 cagccaaagg caatattcag cttcgcaata ccaagctgaa cgcagccaag gctctcgaaa
     5701 ccaccgcatt gcagggcaat atcgtttcag acggccttca tgctgtttct gcagacggtc
     5761 atgtatcctt attggccaac ggtaatgccg actttaccgg tcacaatacc ctgacagcca
     5821 aggccgatgt caatgcagga tcggttggta aaggccgtct gaaagcagac aataccaata
     5881 tcacttcatc ttcaggagat attacgttgg ttgccggcaa cggtattcag cttggtgacg
     5941 gaaaacaacg caattcaatc aacggaaaac acatcagcat caaaaacaac ggtggtaatg
     6001 ccgacttaaa aaaccttaac gtccatgcca aaagcggggc attgaacatt cattccgacc
     6061 gggcattgag catagaaaat accaagctgg agtctaccca taatacgcat cttaatgcac
     6121 aacacgagcg ggtaacgctc aaccaagtag atgcctacgc acaccgtcat ctaagcatta
     6181 ccggcagcca gatttggcaa aacgacaaac tgccttctgc caacaagctg gtggctaacg
     6241 gtgtattggc actcaatgcg cgctattccc aaattgccga caacaccacg ctgagagcgg
     6301 gtgcaatcaa ccttactgcc ggtaccgccc tagtcaagcg cggcaacatc aattggagta
     6361 ccgtttcgac caaaactttg gaagataatg ccgaattaaa accattggcc ggacggctga
     6421 atattgaagc aggtagcggc acattaacca tcgaacctgc caaccgcatc agtgcgcata
     6481 ccgacctgag catcaaaaca ggcggaaaat tgctgttgtc tgcaaaagga ggaaatgcag
     6541 gtgcgcctag tgctcaagtt tcctcattgg aagcaaaagg caatatccgt ctggttacag
     6601 gagaaacaga tttaagaggt tctaaaatta cagccggtaa aaacttggtt gtcgccacca
     6661 ccaaaggcaa gttgaatatc gaagccgtaa acaactcatt cagcaattat tttcctacac
     6721 aaaaagcggc tgaactcaac caaaaatcca aagaattgga acagcagatt gcgcagttga
     6781 aaaaaagctc gcctaaaagc aagctgattc caaccctgca agaagaacgc gaccgtctcg
     6841 ctttctatat tcaagccatc aacaaggaag ttaaaggtaa aaaacccaaa ggcaaagaat
     6901 acctgcaagc caagctttct gcacaaaata ttgacttgat ttccgcacaa ggcatcgaaa
     6961 tcagcggttc cgatattacc gcttccaaaa aactgaacct tcacgccgca ggcgtattgc
     7021 caaaggcagc agattcagag gcggctgcta ttctgattga cggcataacc gaccaatatg
     7081 aaattggcaa gcccacctac aagagtcact acgacaaagc tgctctgaac aagccttcac
     7141 gtttgaccgg acgtacaggg gtaagtattc atgcagctgc ggcactcgat gatgcacgta
     7201 ttattatcgg tgcatccgaa atcaaagctc cctcaggcag catagacatc aaagcccata
     7261 gtgatattgt actggaggct ggacaaaacg atgcctatac cttcttaaaa accaaaggta
     7321 aaagcggcaa aatcatcaga aaaaccaagt ttaccagcac ccgcgaccac ctgattatgc
     7381 cagcccccgt cgagctgacc gccaacggca taacgcttca ggcaggcggc aacatcgaag
     7441 ctaataccac ccgcttcaat gcccctgcag gtaaagttac cctggttgcg ggtgaagagc
     7501 tgcaactgct ggcagaagaa ggcatccaca agcacgagtt ggatgtccaa aaaagccgcc
     7561 gctttatcgg catcaaggta ggcaagagca attacagtaa aaacgaactg aacgaaacca
     7621 aattgcctgt ccgcgtcgtc gcccaaactg cagccacccg ttcaggctgg gataccgtgc
     7681 tcgaaggtac cgaattcaaa accacgctgg ccggtgcgga cattcaggca ggtgtaggcg
     7741 aaaaagcccg tgccgatgcg aaaattatcc tcaaaggcat tgtgaaccgt atccagtcgg
     7801 aagaaaaatt agaaaccaac tcaaccgtat ggcagaaaca ggccggacgc ggcagcacta
     7861 tcgaaacgct gaaactgccc agcttcgaaa gccctactcc gcccaaactg accgcccccg
     7921 gtggctatat cgtcgacatt ccgaaaggca atttgaaaac cgaaatcgaa aagctggcca
     7981 aacagcccga gtatgcctat ctgaaacagc tccaagtagc gaaaaacgtc aactggaacc
     8041 aggtgcaact ggcttacgat aaatgggact ataagcagga aggcttaacc agagccggtg
     8101 cagcgattgt taccataatc gtaaccgcac tgacttatgg atacggcgca accgcagcgg
     8161 gcggtgtagc cgcttcagga agtagtacag ccgcagctgc cggaacagcc gccacaacga
     8221 cagcagcagc tactaccgtt tctacagcga ctgccatgca aaccgctgct ttagcctcct
     8281 tgtatagcca agcagctgta tccatcatca ataataaagg tgatgtcggc aaagcgttga
     8341 aagatctcgg caccagtgat acggtcaagc agattgtcac ttctgccctg acggcgggtg
     8401 cattaaatca gatgggcgca gatattgccc aattgaacag caaggtaaga accgaactgt
     8461 tcagcagtac gggcaatcaa actattgcca accttggagg cagactggct accaatctca
     8521 gtaatgcagg tatctcagct ggtatcaata ccgccgtcaa cggcggcagc ctgaaagaca
     8581 acttaggcaa tgccgcatta ggagcattgg ttaatagctt ccaaggagaa gccgccagca
     8641 aaatcaaaac aaccttcagc gacgattatg ttgccaaaca gttcgcccac gctttggctg
     8701 ggtgtgttag cggattggta caaggaaaat gtaaagacgg ggcaattggc gcagcagttg
     8761 gggaaatcgt agccgactcc atgcttggcg gcagaaaccc tgctacactc agcgatgcgg
     8821 aaaagcataa ggttatcagt tactcgaaga ttattgccgg cagcgtggcg gcactcaacg
     8881 gcggcgatgt gaatactgcg gcgaatgcgg ctgaggtggc ggtagtgaat aatgctttga
     8941 attttgacag tacccctacc aatgcgaaaa agcatcaacc gcagaagccc gacaaaaccg
     9001 cactggaaaa aattatccaa ggtattatgc ctgcacatgc agcaggtgcg atgactaatc
     9061 cgcaggataa ggatgctgcc atttggataa gcaatatccg taatggcatc acaggcccga
     9121 ttgtgattac cagctatggg gtttatgctg caggttggac agctccgctg atcggtacag
     9181 cgggtaaatt agctatcagc acctgcatgg ctaatccttc tggttgtact gtcatggtca
     9241 ctcaggctgc cgaagcgggc gcgggaatcg ccacgggtgc ggtaacggta ggcaacgctt
     9301 gggaagcgcc tgtgggggcg ttgtcgaaag cgaaggcggc taagcaggct gctcctaaag
     9361 aaacaataaa caatttggca aatttagcca aagcagaaca gcagatttta ttccgtattg
     9421 cccaacgcga tacgcaactg gatgcatgga agacgggatt taacaataga gtaaggaaag
     9481 gagcaggctt gcttgatgca agtaatattc cgataaccat taacggaaaa accatcaaac
     9541 ctgtacaagc cataagctta aagggagcac ccgtttacag cggcgtaagc gaacaggaga
     9601 tttttgcgct ttatcggcag atgactggcc agaatccgaa ttttagagtt ttgcctgacg
     9661 gaagattagc aaatggcatt atcagtactg gagaatgggc aggaacaaaa attgcattaa
     9721 gaaatttttc aaaaacagag aattcaactc aagcacgatg gacattagat ttgcagaatc
     9781 ctccatcatt tattaaaggt actaaattgg agcttaaatt ccaataattt acaaaggatt
     9841 ttaccgtgga tgagaaacaa aaaattaaga ttcttgattt tcaaatcgat ttatcctcaa
     9901 tttttaactc ttataaaaat caaatgggta ttaatattca agatgaaaac ttaaaaaaca
     9961 atttctgttc ttttatggaa gaactgttaa atgacggttc aatccgttta catgattata
    10021 ccgacggtat cggaattcct ctaactggaa cttcaaaaga acaagtgcag aaattgaaag
    10081 acatatggcc tactttggaa gatgcccaag caatatggcc tgaagaccct tggtattact
    10141 tagaatggct ttggtgggat attgcgtgtc caatagattt ggccgatttg ccgaatattg
    10201 atatttatga gcaagcgtag gtatggttag ccgcctttag cggcgtaacc gtacgcatat
    10261 cagcaaactt tataaaataa caaggccgtc tgaaatctgt ttttcaactt tttcagacgg
    10321 ccttgcaact tggcatttca ttcgtacggt tacgccgcta aaggcggcta accgtaccta
    10381 cgagctctga taaaaatgat ttatggaagc aagctgtagc ctgcatgaaa cctaaaatcc
    10441 atgcgtaagg tgtgtgcttc agcgcgcacg cgttccatga tttacggctc aatgccgtct
    10501 gaaaagctca attttttcag acggcatttg ttatgcaagt aaatattcag attctctgta
    10561 tactgttcag acgcgtgcgt gctgaagaca cctcctacgc ttgctgcaga actttcgggt
    10621 aaaaccggtg tgagcattag cgcgccgtat gccaatgaaa acagccgcat cctgctgagc
    10681 accacggata tcagttcgga aaacggcaaa atcaaactgc aatcctacgg cgaccagttc
    10741 tactacgccg gacagggtga gctctacacc ttcgataaac gcagctataa aaccggtaag
    10801 tggtacaaac taaaacatgt tactgaaatc aaagagcata aaaacgccaa agccgacccg
    10861 gtgagcctca gtgcgtcaca aggtattgaa atcaaatccg gcggcaatat cggtgcccac
    10921 gccaccttgt ttgatgcacc ccgcggctcc gttaaaatcg aagccggacg tgggctggtt
    10981 ctctatgccg tggaagatct caactacgac aaacttgaca cccgtaccaa gcgcaaattt
    11041 atcggcatta cctacgacaa ggtgcacgac accaccaccc acaccatgaa aaccgccctg
    11101 ccctcaaggg tagttgcaga atctgccaat ctgcaatcag gttgggatac caaactgcaa
    11161 ggcacacagt ttgaaaccac actgggtggc gcaaccatac gcgcaggcgt aggcgagcag
    11221 gcacgggccg atgccaagat tattcttgaa ggcatcaaaa gtagtgtgcg cactgaaaca
    11281 gtaagcagta gcaaatctgc cctctggcag aaacaggccg gacgcggcag caatatcgaa
    11341 accttgcaac tgccaagttt cacaggctcc gttgcgcccg tactctctgc ccccggcggc
    11401 tatatcgttg atattccgaa aggcaatctg aaaaccgaaa tcgaaaagct ggccaaacag
    11461 cccgagtatg cctatctgaa acagctccaa gtagcgaaaa acgtcaactg gaaccaggtg
    11521 caactggctt acgataaatg ggactataag caggaaggct taaccagagc cggtgcagcg
    11581 attatcgcgc tggctgttac cgtggttact gcgggcgcgg gagtcggagc cgcactaggc
    11641 ttaaacggcg cagccgcagc agcggccgat gccgcctttg cctcactcgc ttctcaggct
    11701 tccgtatcgc tcatcaacaa taaaggcgat gtcggcaaaa ccctgaagga actgggcaga
    11761 agccgcacgg taaaaaatct ggttgtagcg gcggcaacgg caggcgtatc caacaaactc
    11821 ggtgcctctt cccttgccac ttggagcgaa accccttggg taaacaacct caacgttaac
    11881 ctggccaatg cgggcagtgc cgcgctgatc aacaccgctg ttaacggcgg cagcctgaaa
    11941 gacaatctgg aggcaaatat cctggcggca ttggtgaata ccgcgcatgg ggaggctgcg
    12001 agtaagatta agcaattgga tcagcactat atcgcccaca aagttgccca tgccgtagca
    12061 ggctgtgcag cagcgacggc gaataagggt aagtgtcagg acggtgcgat tggcgcagcc
    12121 gtgggtgaga ttgttgggga agctttggtt aaaaataccg attttagcga tatgaccccg
    12181 gaacaattag atctggaagt taagaaaatt accgcctatg ccaaacttgc ggcaggtaca
    12241 gttgcaggcg taacgggagg agatgtcaat actgctgcac aaaccgcaca aaacgcggta
    12301 gaaaataatg cggttaaagc tgttgtaact gctgcaaaag tggtttataa ggtagccaga
    12361 aaaggattaa aaaacgggaa aatcaacgtt agagatttaa aacagacgtt gaaagacgaa
    12421 ggttataatt tagccgacaa cctgaccacc ttattcgacg aaacattgga ttggaacgat
    12481 gccaaagccg ttattgatat tgtcgtcgga acagagctga atcgcgctaa taaaggggaa
    12541 gcggcacaaa aggtcaagga agttttagaa aaaaatcgtc cttatatccc taataaaggt
    12601 gctgtaccga atatgagtac atacatgaaa aataatcctt ttggaaaaca gctggctcaa
    12661 atttcagaaa agacaacgct tccgacgcag caagggcagt ctgtcttctt ggtaaaaaga
    12721 aaccaagggt tattaaaaac cggtgatagg ttttatttag atggccaaca taaaaatcat
    12781 ttagaggttt ttgataaaaa tgggaacttt aagtttgttc taaatatgga tggttcgctt
    12841 aaccaaatga aaactggggc agcaaaaggt cgtaaattaa acttaaaata ggaaatttta
    12901 tggaaacatt gaatgatata aaaaaaatct tgattaatgt ggggctttat caagggtttg
    12961 atttgacaga tccaaaagta tcagaagaag ttaatcatga aacagctaat atgaaatgga
    13021 ttaaagatta tacttcagac gggaattggg ataatgaatt taaggaggat ttaaaaaact
    13081 ttttagatta tatggaagta tgccaattag ccctaaacga taaaaatttc aaaattgcca
    13141 gtaattcttt atttatggct atgatttacg caggtaatct atctcttata tttgattcaa
    13201 taaaaactga tatatcaaca ttattgagtg ctgagtataa aaagaatagt ttttcatggc
    13261 catctcttga tgaatagaaa gcaagttgta gcctgcatga aatctaaaac ccatgcataa
    13321 ggtgtgggct tcagtatacg cgttccatga tttacggcca tatgccgtct gaaaagctca
    13381 attttttcag acggcatttg ttatgaaagt aaatatttag attccctgta tactgtttag
    13441 actcgtgtgt gctgagtaag ctgtagtctg catgaaacct aaaactcgct caaaattaag
    13501 ctaagacatt agcagggcaa gggcgaaaat tgaatcttaa ataaggtgat tcagatgaaa
    13561 aattttaatg tagtaaaaga aagtttaaga gagttaggaa ttaaacaagg atttgatctt
    13621 tatgagaaag ccacaactga aaaattgaat agtgaagatc ctcttgactt acaatggctt
    13681 tctaactatt catctgattg gaatgatgaa ttagaagaag actttgattc tttttttcag
    13741 catatgaagg aatatcaata tgctattgac aatgaagaca ttaaatctgc atgtagttca
    13801 ctatgtgaag ctatgctcta tgttggtaat attaaaaatt tttttgagtt tctcaaaagc
    13861 gatatgatta gactgttgag aggtgaaagt aaaacaacag actttcaatg gccgcaattt
    13921 gatgaatagc agcaagctgt agcctgcatg aaacctaaaa tccatgcgta aggtgtgtgc
    13981 ttcagcacgc acgcgttcca tgatttacgg ctcaatgccg tctgaaaagc tcacaatttt
    14041 tcagacggca tttgttatgc aagtaaatat tcagattccc tatatactgc ccagatgcgt
    14101 gcgtgctgaa gacaccccct acgcttgcta tttgaaacag ctccaagtca ccaaagacgt
    14161 caactggaac caggtacaac tggcgtacga caaatgggac tataaacagg aaggcttaac
    14221 cggagccgga gcagcgatta ttgcgctggc tgttaccgtg gttactgcgg gcgcgggagc
    14281 cggagccgca ctgggcttaa acggcgcggc cgcagcggca accgatgccg cattcgcctc
    14341 gctggccagc caggcttccg tatcgctcat caacaacaaa ggcaatatcg gtaacaccct
    14401 gaaagagctg ggcagaagca gcacggtgaa aaatctgatg gttgccgtcg ctaccgcagg
    14461 cg
//
LOCUS       DAWGRZ010000037        14414 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000037 DAWGRZ010000000
VERSION     DAWGRZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14414
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.037"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..158
                     /locus_tag="WHT44_000770"
     CDS             <1..158
                     /locus_tag="WHT44_000770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532983.1"
                     /translation="HLYSPSLQPSLQPSLQPSLQPSLQPISTTRLYNPSLQPSLQPVS
                     TAISTAR"
     gene            complement(155..2128)
                     /locus_tag="WHT44_000771"
     CDS             complement(155..2128)
                     /locus_tag="WHT44_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ2532984.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVITGDQTVNGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHGANNRISYNNHDIFHKANKPRFAEDIQGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAVGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVQNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWTNHTDAA
                     ALGYEDRNERQRSALVSTWTDENLNDNGFLTPRSDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQGLQDKADRDHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSERVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(2139..2699)
                     /locus_tag="WHT44_000772"
     CDS             complement(2139..2699)
                     /locus_tag="WHT44_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ2532985.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQHRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            complement(2696..3307)
                     /locus_tag="WHT44_000773"
     CDS             complement(2696..3307)
                     /locus_tag="WHT44_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ2532986.1"
                     /translation="MAAPAGVATECRLTVQGGTDRESDASLLARLLEIIRRPPAGGNR
                     YDYKNWALSVDGVTSAYVYPLRRGLGTVDIAITSADGVSSEETVRRVQAYIDEMRPVT
                     AKNALVLKPTVTAVPVTVQVKLDGIDLDEAKRRIRTALKEYFDTLIPGDGLTVSQIEA
                     AISNVDGVIDRRLTAPTANRAADTVNRIEWFKAGAINVTEMPS"
     gene            complement(3307..3750)
                     /locus_tag="WHT44_000774"
     CDS             complement(3307..3750)
                     /locus_tag="WHT44_000774"
                     /inference="COORDINATES: protein motif:HMM:NF016736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ2532987.1"
                     /translation="MFETPTFEQIRERILRDTKSLWPDADISPDSDHYVHASRLASCA
                     EGQYAHQSWIVRQIFPDTADREYLERHASMRGLSRRNPTTASGTLTVSGIAQSMLSDD
                     LQVRIGQRFYRTTARAVIGSGGTAEIPAIADEPGAAANVATARRN"
     gene            complement(3764..4111)
                     /locus_tag="WHT44_000775"
     CDS             complement(3764..4111)
                     /locus_tag="WHT44_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HEZ2532988.1"
                     /translation="MDKELNPSIGDYTGRTVDTLQNAVYIRLMTPLGSWWADKTLGSL
                     LHLLQREKDLQRVSLLAEQYADEALQPIVKSGRADKITVRAEQPHDGRLILHIRMDTA
                     AGGFDYRHEVPVI"
     gene            complement(4212..4880)
                     /locus_tag="WHT44_000776"
     CDS             complement(4212..4880)
                     /locus_tag="WHT44_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEZ2532989.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPDGSEAVVIPLGGNTSHGVIVCSQHGSYRIKNLKPG
                     ETAIFNHEGAKIVIKQGKIIEADCDVYRVNCKQYEVNAATDAKFNAPLVETSAVLTAQ
                     GQINGNGGMAVEGGDGATFSGDVNQTGGSFNTDGDVVAGNISLRQHPHTDSIGGKTLP
                     AEPA"
     gene            complement(4877..6022)
                     /locus_tag="WHT44_000777"
     CDS             complement(4877..6022)
                     /locus_tag="WHT44_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ2532990.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGR
                     LGPEAAIPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGSRELSLSGRDLAGFLVDCS
                     APQLNVKGMTVLDAAKKLAAPWPQIKAVVLKAENNPALGKIDIEPGETVWQALTHIAN
                     SVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRCNIERMDIEWDTDNRFSEVTF
                     LAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKKQLADWRLE
                     GFTLTITVGGHKTRDGVLWQPGLRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELR
                     LKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            complement(6012..7343)
                     /locus_tag="WHT44_000778"
     CDS             complement(6012..7343)
                     /locus_tag="WHT44_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ2532991.1"
                     /translation="MSGWHTLLQDASYKGVGFDIEVVDESNGKALAEHARPFVQGIDL
                     EDMGMTGRQVQINAVFWGKGYAGRLKKLLDALEQPGGGVLVHPVWGRMHNMIAASWSY
                     RHEADYVDYAGIDITFREAAEAQEIFVFENAFLVELEALIANIDTYREAAIGFVDAVL
                     AVDAGVSALWGSALGIWSAASGTFGAVRRLFDLDKIAFPDRGGYSAAAFKNGSAKLFA
                     DISVMVDTGIRREAGLADNAMHHAGWSPRQRFDGAAAVADRAAAIPDNLLTGRFSDGL
                     QNRLNRLTAKQVQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMRRRM
                     QAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGRLNALVAAVINQKPPLI
                     VRQAPIDGTIHQIAHEFYGDIARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            complement(7343..9514)
                     /locus_tag="WHT44_000779"
     CDS             complement(7343..9514)
                     /locus_tag="WHT44_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein F"
                     /protein_id="HEZ2532992.1"
                     /translation="MANGNMKLSLVLTARDDGARRLLADTQRQLDRTAKSRAQLERQS
                     HTYALTGIRSEKQIQREIMLTQAAFNRLARSGKASQNDLARAAVATRNRIRELNAELK
                     QGTGFADKMGKIGRFGAAAVAGGAAAYTVLKPAMDNRKQLDENINRVSRQAFIEDNSK
                     SAAWIATEGAQQIKDLALELVEKNGGTHDKALDLISGMMTTGLNFAQTKNEAQAAYAF
                     ALASEGSGEDTAKLIKTLKDGGMSGKDLQLGLEHVLQSGLDGTFEVRDMVRELPSLLS
                     AAQQAGMNGVGGLDYLLSLLQSAANKSGSPAEAATNVQNLLSKTLSPDTIGRLKKMAN
                     PNDPKKGVDWIGSVVQGKQNGENAVQVLSRLADAMLVKDKQYQDYKKRAAAGDKTAAE
                     QANMLKGALLAQLLPDLQAKQGLLAATDMTQIREYMASLAGVTLDNGKIAKNNEARML
                     SAAAQQEQQESLAMLRESLTGTLVDMETSFKKLAAEYPNATLALQALTTAATAASAAM
                     LLTAGGGKGAGFLKDVGSKALGWGKASAGGVAAGATAAGGKLLSWGKSAGSGLMNNPA
                     LVKRAGLLGMLLYSESLGDGTLPKGLRGTKTTPEMINRLKNNGIRFEPAPKREQARGG
                     VPQYLAAPSAQPTDKMLSPLFSTQTAAYQAAIQQQTAAYQAALAQDTAAVTTGLAQVQ
                     SAMASASQTINTNVSLNIDGRVIANEVSRYQVAMFGRGAGQ"
     gene            9669..10271
                     /locus_tag="WHT44_000780"
     CDS             9669..10271
                     /locus_tag="WHT44_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532993.1"
                     /translation="MNMKKLISAICVSIVLSACNQQSKTAQAEEPVQSIQAADCTAPM
                     DITVEQYLINLEQAFKTQNVSTKIHNKNIVKTDCGYDLTLVMDFGAIALKLDEQQKIR
                     AISVGYILKTDGEKGQNLVNNAINGLHSIQAVLSLTTTDKLGESEAGKQLFTALTEVV
                     KESNQTGATAQKDVPADGILYSVVFEKETNTIAIIGRKQP"
     gene            complement(10315..10548)
                     /locus_tag="WHT44_000781"
     CDS             complement(10315..10548)
                     /locus_tag="WHT44_000781"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532994.1"
                     /translation="MRRRRRSSKTQLPDGTQKLPSGGHPDGKAGIGADAVRDMCHAEL
                     SAWFEDILSSLGIKTPKEEGVIVSKRLRKPEGK"
     gene            complement(10523..10888)
                     /locus_tag="WHT44_000782"
     CDS             complement(10523..10888)
                     /locus_tag="WHT44_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2532995.1"
                     /translation="MITVKLTHGLTYNGKVVSELRLKPLTVGGELAAFALIDDLPELP
                     ENATKAELLQRDVLETLTYWSQQIEAQGIPSDILTAQWLMENLSTEDYHTVMAAQEDL
                     RLKPSAATASPDAPSAAEQ"
     gene            complement(10892..11269)
                     /locus_tag="WHT44_000783"
     CDS             complement(10892..11269)
                     /locus_tag="WHT44_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ2532996.1"
                     /translation="MSDATYADAVIMEMNGRDIEIVSIKPQTTTGRKPVKTMNRNGRV
                     NGYCDGVTEHKLSVTAAIPIDGTEIDWDNITKAKITIYPINDEDRRTSYLDCFTVDTG
                     EQYEVDNEARIDIEMIALHKIKE"
     gene            complement(11334..12743)
                     /locus_tag="WHT44_000784"
     CDS             complement(11334..12743)
                     /locus_tag="WHT44_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEZ2532997.1"
                     /translation="MPHIDFDTIPGSIRVPGQYIEFNTRNAVQGLPQNPQKVLMVAPM
                     LTAGIQPALEPVQLFSDAEAADLFGQGSLAHLMVRQAFANNPYLDLTVIGIADHSAGV
                     QATATVTLSGTATAPGVVEITIGGKQVSTAVNTGETAATVADRLKTAITAADVTVTAS
                     GSGAAVTLTAKHKGEIGNESGLTVSTGNTGLTYQANAFTGGAKNADIATALSKVAGKH
                     YHIICSPFSDDANAKALSNHITNVSNAIEQRGCIGVLGMSAALSTATTATGEINDGRM
                     TCAWYKGAVEPNGIIAAGYAAVLAFEEDPAKPLNTLEIKGLAVTPDAQWPLFAECNNA
                     LYNGLTPLTVVNNRVQIMRAVSTYTKSANNTDDPALLDITTIRTLDYVRRSVKERIAL
                     RFPRDKLSDRLLPKVKSEILDVLIKLDQAEIIENAEANKGKLVVARAQNDPNRVNAII
                     PADVVNGLHVFAGRIDLIL"
     gene            complement(12746..12943)
                     /locus_tag="WHT44_000785"
     CDS             complement(12746..12943)
                     /locus_tag="WHT44_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ2532998.1"
                     /translation="MSKIKVTAADGLRVPTEHNPHEYIGQEPVEVDGNSLYYRRMIDD
                     GDLVVVEDAAPNTKTRNTKGE"
     gene            complement(12940..13590)
                     /locus_tag="WHT44_000786"
     CDS             complement(12940..13590)
                     /locus_tag="WHT44_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ2532999.1"
                     /translation="MITDIEQAITDRLKRGLGRMVRTVKSYNGEADDLAGQIHTLPAV
                     WVTYGGSKVEPASTGGVCGRYQDTAEFVVMVAARNLRNEQAQRQGGIDSREIGSNDLI
                     RAVRRLLDGQRLGFADSRGLVPKAVRAIANHVLVQNAAVSIYAVEYAIRFNTCGLEND
                     RYPERTDNPDDPNHIFTKYQGTLSEPWPDFEGLDGKIYDPQSADEIPVNLTLKDKQ"
     gene            complement(13580..14002)
                     /locus_tag="WHT44_000787"
     CDS             complement(13580..14002)
                     /locus_tag="WHT44_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ2533000.1"
                     /translation="MAYATVEDMVARFGELEVLQLTDRNNEGLIDREVAQTALVDATA
                     EIDAYLGRFRRPFEDLPPILVRLCCDIARYRLTAAQGVLITDEIRNRYKIDVLDLLRA
                     MAKGEVQLGVDDSGEEVAAGEDGIVFVNGKNKVFGRDH"
     gene            complement(14002..14400)
                     /locus_tag="WHT44_000788"
     CDS             complement(14002..14400)
                     /locus_tag="WHT44_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533001.1"
                     /translation="MAKTNNKPETAETAAPSFEDIKAELDAVQAELAAARNDVEMLTT
                     ALEKAEDDKKALSAELAELKVQHTQRAADALADSRDVMLVSTGADGKEFWRGGLLFDG
                     GWREVKRAEVGEAVWKAICAEPMLQRKAVE"
BASE COUNT         2804 a         4192 c         3898 g         3520 t
ORIGIN      
        1 cccatctcta cagcccatct ctacagccat ctctacagcc atctctacag ccatctctac
       61 agccatctct acaacccatc tctacaaccc gtctctacaa cccgtctcta cagccatctc
      121 tacagcccgt ctctacagcc atctctacag cccgctagtc ttgcagtgcc tcgagcaagc
      181 cgtctcggcg gcggcggttt cggggtccgg ttgatgttct tccactctct ctttgcggtt
      241 cttcgttggt ttcaggtatt tcggggccta tcggggagct ggcggcgttt cccgaggcgg
      301 ctttgccgat ggccatccag tcgcaactgc cttcgtacca gttggtgata atggcggcgg
      361 cgtggttttt tgcgcggata aacagccagt tgtgttgcct tcgatcgccg gtaacgcgtt
      421 ccgaatggcg ttcggatacg aagcatttga cgttgccgtc ggcaaaggcg acggggaaga
      481 cgacttcgtt ttcgatgggg ccgccgcttc gtgtgaacct gtagctgccg gtctgtatca
      541 ttgtgccgtc ggggaatctg acaatatcca cgccgccgat ggtttggcgt gtgaattgcg
      601 cggcaacggc ggtgctgatt ttttcgtcca gcccttggat ttgggcggcg gtgtgggtgt
      661 ggtctcggtc ggctttgtct tgcaaacctt gcgccagcgt gcccgcgtcc agcgcgcctg
      721 cgttgatggt ttcgccgtgg gactttatcc aaaacacggc ttcgcccaag gtgtcggcgg
      781 ctttgatgca cagttttaaa accagggctt tggggcggtt ttcgtcgccg cctgtcgcca
      841 ttttgctgtc cgagcgcggg gttaaaaagc cgttgtcgtt taaattttcg tccgtccaag
      901 tcgatacgag cgcgcttctc tgcctttcgt tgcggtcttc gtaaccgagg gctgccgcgt
      961 ctgtgtggtt tgtccagtgt gaaaatactt tgtggacgtg ccgtttgatt tcgtcttcct
     1021 gcttcgttcc gactgccaag ctgttgcccg cgttgcggat aaagcggtct tccgcctgcg
     1081 ggacgttttg gatgctgccg tattttccgg tcagcagacg gtacagctcg ggataagcgg
     1141 tttcggttac gcgcgtgcgg atgtcgtcaa acgccagcca gccggtcggg atttggtcgg
     1201 acggaaacca cgcggtgatg ccgatgtcgg tacggcttaa atcgggcagg cggttgctgt
     1261 tgcccaaggc gcggtaaagg tcggggaagg tgttttgtgc aaaggtcgtg ccgttggctt
     1321 tgagatagcc tgcggggttt tgtacggctt tgggaaagga tacgatcgcg ccgacgggga
     1381 tgccgtccga attgagtttt ttccatgcgg accagttgtc gtttgcgctg ctcgtttggt
     1441 ggcgttcgta cacgtcggac gtgtaggcgg gataacccaa ctgcctgcac cagccgtgtt
     1501 tcgtgccggc gatgacttgg atatggcagg cggtgttttc aacgggcagg ttggagctgc
     1561 cgaccgccgt cggcagggaa tagatgccgt ctgttttgag ggtgttcaaa tcgccccgga
     1621 atgtttcgac tttgaaattg ccgatgccgt atccggacag tgtgttcggt ttgccttgga
     1681 tgtcttcggc aaaacgcggt ttgttggctt tgtggaagat gtcgtggttg ttgtaggaaa
     1741 tgcggttgtt tgcgccgtgc aggcggaggg tgtcgtcgcc gaagacgatg tgggcgttgt
     1801 cggcatccgc gccgatgtag gctttgtggc cgccgttcca gttcgtctgg ttggcggata
     1861 aatggatgcc gctttggaat tgggtttggg cggtaaaggt tttttggccg ttgacggttt
     1921 ggtcgccggt gataactaca gccagcctat ttagtgctgc cagtaattga ttaggctgcg
     1981 acttatccgg ctggataccg gccgcagtca gcacggccag cagctcggat tgcacttggt
     2041 tgagccacca cgcgggcagg atggtgccca attcggacac gccgtcgccg tcgtggaagg
     2101 tcttatcggg ggtttcgatg gggtgcataa atcgtccttt agcggtaggt aaatcggata
     2161 gcggtgtggg cgggcttgag gcggttgaac aggctctcga tcacggcatc gctgtaatcg
     2221 gtcagcctgt cgcccgccgc cgagataccg gcgcggaatc gggtaatgcg gttgttgccg
     2281 ccgcgcacgt taacgtgcca cacccacatg atttcctgcg gcgcaagacg gtcgccggcg
     2341 cggtttacac cggcgcggaa cggctgcggt tcgtcgattt ggatttgata gcccgccgcc
     2401 tcggccaaac gcacaaaata aggaatactc aagccgcctg tttcgtttag cttggccatg
     2461 acggccaaca cacggtgctg gcggtttttg cccgtaccgt ccaaacctaa tacgcgctcc
     2521 caatcggcca gcatttggcc ggcgctgcgc gggtcgacgg catcggcaac gctttgagcc
     2581 gattccgcca ccgcatccag cgctgcgccg tctatttctg cctgcgcccg cacacgcggg
     2641 gcattgcggg catacgacac ggggggcaac aggccccgca agatgtcttg atagctcatg
     2701 acggcatctc cgttacatta atcgcgcccg ctttaaacca ctcgatgcgg ttaaccgtat
     2761 cggcggcacg gttggccgtc ggcgcagtca gacggcggtc gatcacacca tccacattgc
     2821 tgatagcagc ctcgatttgc gacacagtca ggccgtcgcc ggggatcagg gtgtcgaaat
     2881 attcttttag ggccgtccgt atgcggcgct tggcctcgtc caagtcgata ccgtcgagct
     2941 tgacttgcac ggtaacaggc accgccgtta cggttggctt gagtaccagc gcattttttg
     3001 ccgttaccgg gcgcatctcg tcgatataag cctgtacgcg gcgcacagtt tcttccgacg
     3061 acacaccgtc ggcggaggta atggcaatat ccaccgtacc caagccgcgg cgcagcggat
     3121 aaacatatgc gctggttacg ccgtcaacac tcaacgccca gtttttatag tcgtaacggt
     3181 tgccgcctgc gggcggtcgg cggatgattt ccaacagacg cgccagcagt gaggcatcgc
     3241 tttctcggtc ggtgccgcct tgtacggtaa ggcggcattc ggtggccaca ccggcggggg
     3301 cggccatcag ttgcgcctcg ccgtcgccac attggcggcc gcgcccggct cgtcggcgat
     3361 tgccggtatt tccgccgtgc cgccgctgcc gataacggcg cgggcggtag tgcggtaaaa
     3421 acgctggccg atacgcactt gcaggtcgtc tgaaagcatg gattgcgcaa taccgcttac
     3481 ggtcagcgtg ccgctggccg tggtaggatt gcggcggctc aagccgcgca tggaggcatg
     3541 ccgctccaaa tactcgcggt cggcggtatc agggaaaatc tgccgcacaa tccagctttg
     3601 atgcgcatat tgcccttcgg cgcagctggc caaacggctg gcgtgcacat aatggtcgct
     3661 gtcggggctg atatcggcat ccggccacag gcttttggta tcgcgcagga tacgctcgcg
     3721 gatttgctca aatgtcggcg tttcaaacac gtttaaaacc tctttaaatc acgggcactt
     3781 cgtggcggta atcaaacccg cccgccgccg tatccatccg gatatgcagg atcaggcggc
     3841 cgtcgtgcgg ctgctctgcg cgcacggtaa tcttgtcggc acgcccgctc ttaacaatcg
     3901 gttgcagtgc ctcatcggca tattgctcgg ccaacaggct gacccgttgc aggtcttttt
     3961 cgcgctgcaa caaatgcagc agcgagccga gcgttttatc cgcccaccag ctgcccaacg
     4021 gtgtcatcaa gcggatatac acggcatttt gcagcgtatc gacggtgcgg ccggtatagt
     4081 cgccgatgct ggggtttagc tctttgtcca tgttggcatt ttgccgtttt cagacggcct
     4141 cacggagtac acgggcttca atcccacacg cacaaaaaaa gcagcccgaa ggctgccaaa
     4201 ggtctgcttg tctatgccgg ttccgccggt aaggttttgc cgccgatgct gtcggtatgc
     4261 gggtgctggc gcaacgatat attgccggcc accacgtcgc cgtcggtgtt aaagctgccg
     4321 cccgtttggt taacatcgcc gctaaaggtg gctccgtcgc cgccctcgac ggccatgccg
     4381 ccgttgccgt tgatttggcc ttgcgccgtc aacactgcac tggtctccac caacggagcg
     4441 ttaaatttgg catccgtggc cgcattaacc tcgtattgtt tgcagttaac ccggtacacg
     4501 tcgcaatcgg cctcaatgat tttgccttgc ttaatcacga tttttgcacc ctcatgatta
     4561 aaaatcgccg tctcgccggg cttaaggttt ttgatgcggt agctgccgtg ctggctgcac
     4621 acaatcacac cgtgcgaagt attgccgccc agcggtatca ctaccgcttc gctgccgtcg
     4681 ggcggatggc tggcaaagcc gtattcctgc aaatgttcaa ggtcttgcag ggtttcgtcg
     4741 gccaagccgc tcaactgcac gcgctgtatc ggctcggacg acaccaccag cgtgattttt
     4801 ccccgaaagg ccgcgcgcag ggtttcgccg atatttttag cagtttgtgc cgtttttttc
     4861 gccaatttac tcaaactcat tcaaacaccg ccgtttctgc ttgttttttg ccgccttttt
     4921 tgcccttatg gctcacgcct ttgcgtttgc ctttgcgctt gcgcgccgcc tcggcctttt
     4981 tggggtaagc gtcgggtgtc caaataccgt cctctttgag ccgcagctcg gtttgcgtac
     5041 catccatgcg ggatagcata aaccgccgcc ccatcagaaa aaacaccgca tcgataccgt
     5101 gctcgtcgtc gatcacatgc acacgcaggc caggttgcca caatacgccg tcgcgggttt
     5161 tatggccgcc cacggttatc gtgagtgtaa atccctccag ccgccagtcg gccagctgct
     5221 ttttagcctg cttttgcaat gcggccaaat tgtcggcatc ggacaccacc accgttttag
     5281 ggcggtgcag cgtcatcgtc gggtctttgt acacccactt taaatcgtgt ttggcgctgt
     5341 cgccgctgcg gccgtgcgat tgcgccaaaa aagtaacctc ggaaaagcgg ttgtcggtat
     5401 cccactcaat gtccatgcgc tcgatattgc agcggctgtc ggtgcggctc caacacaatg
     5461 tcgccaccgg cgggctgctg taatccgcac cgcccaccac caacgtgccg tccggctcca
     5521 gccacggatg cagcccgacc gagttggcaa tatgggttaa tgcctgccat acggtttcgc
     5581 ccggctcgat gtcgattttg cccaaagcgg ggttgttttc ggccttaagc accaccgctt
     5641 taatctgcgg ccacggcgcg gccagctttt tggctgcatc caataccgtc atgcccttta
     5701 cattgagctg cggcgcggag caatccacca aaaaaccggc caaatcacgc ccgctcaagc
     5761 tcaactcgcg gctgcccttg cttttgccgt ggcgctggct gccgatgatg cccgtcatca
     5821 cgatttgccc gtctatcact acctcgcagc tctctccgct taaatcgggt atggccgcct
     5881 ccggtcccaa cctgccgatg acaaaatcga agctgtcggc agggattaaa aagtcgctgt
     5941 cgatgtcgta gcgctcccag tggcggtgct ctttaccgcc cacgcgcacc gacacggcat
     6001 agccgtatga attattttgc atagctgttg accaaagtgc cgcgcttgat aaacgcgggg
     6061 tggtggatat ggggattgag ccgcaccagc tctgctgcgc gggctatatc gccgtaaaac
     6121 tcgtgggcga tttggtgtat cgtaccgtcg attggggctt ggcgcacaat cagcggcggc
     6181 ttttggttga tgaccgccgc aaccaacgca ttcagacggc ctgccgccgc gcgcagggat
     6241 tctgccgttt ggtaagcctc ggtatacacg gcgttggccg tcagcccacc agactcggca
     6301 gcagccgtct gcaccgcccg caaggcggca atctcggcct gcatacggcg gcgcatggcg
     6361 cggttaacct caatcaaatc gggcgcggtc atctcttcgc catgcgcctc gattaatgcc
     6421 gttgccaccg acaacagcga tgacgtggat aacaggcgca ccgcctgcgc taccggctgc
     6481 acctgtttgg cggttaaccg gttcaggcgg ttttgcaggc cgtctgaaaa gcggccggtc
     6541 agcaaattat cagggatagc ggcggcgcgg tcggcaacag ccgcagcccc gtcaaaccgc
     6601 tgtcgcggcg accaaccggc atggtgcatg gcattatcgg ccaaacccgc ctcacggcgt
     6661 atgccagtat ctaccatgac cgatatatcc gcaaacagct tggccgagcc gtttttaaac
     6721 gccgctgcac tgtatccgcc ccgatcggga aaggcaattt tgtccaaatc aaacaaacgg
     6781 cgcaccgcgc caaacgtacc cgatgccgca ctccaaatgc ccagcgcgct gccccataaa
     6841 gctgatacgc ccgcatccac cgccaacacc gcatcaacaa agccgatagc cgcctcgcgg
     6901 taggtgtcga tattagcaat caacgcctca agctcgacca aaaaggcgtt ttcaaaaaca
     6961 aagatttcct gcgcttcggc cgcctcgcgg aaagtaatat cgatgcccgc ataatccaca
     7021 taatcggcct catgtcggta actccatgat gccgcaatca tgttgtgcat ccgcccccaa
     7081 acagggtgca ccagcacgcc gccgcccggc tgctccagcg catccagcag ctttttcaga
     7141 cggcctgcat agcccttgcc ccaaaacacc gcattaatct gcacctgccg cccggtcatg
     7201 cccatgtctt caaggtcgat accctgcaca aacggccgcg catgctcggc caatgccttg
     7261 ccgttgctct cgtccaccac ctcaatatca aagccgacgc ccttgtaaga tgcgtcctgc
     7321 aataaggtat gccatccgct cattattgac ccgctccacg gccgaacatg gccacttgat
     7381 accgcgatac ctcattcgcg ataacacgtc cgtcgatatt caggctcaca ttggtattga
     7441 tggtctgact tgccgacgcc atcgcacttt gcacttgtgc caaacctgtt gtaactgcag
     7501 ccgtatcctg cgccaatgct gcctgatacg ccgccgtctg ctgctgaatg gctgcctgat
     7561 acgccgccgt ctgagttgaa aacaacggag acaacatctt atcggtaggc tgcgctgacg
     7621 gagcagccaa atactgaggg acaccacccc gcgcctgttc ccgcttcggc gcaggttcaa
     7681 atcggatacc gttgtttttc agacgattaa tcatttcagg agttgtcttg gtaccacgca
     7741 aaccctttgg caatgtgccg tcacccaaag actcggaata cagcaacata cctaacaaac
     7801 ccgcccgttt aactaacgct ggattattca tgagcccgct accggcagat tttccccatg
     7861 acagcaactt accgcctgcc gctgtggcac ctgctgccac gccgcctgcg gaagccttac
     7921 cccatcccaa cgctttacta cctacatctt tcagaaagcc tgcaccttta ccgccaccgg
     7981 cggttaataa cattgcggca gacgccgctg ttgccgccgt cgtcaatgct tgcagggcta
     8041 gagtggcatt agggtattcc gctgccagct ttttaaacga ggtttccata tccaccaatg
     8101 ttcccgtcag actttcccgc aacattgcca gcgattcctg ttgctcttgt tgcgccgctg
     8161 ccgacaacat tcgcgcctcg ttgttcttag caatttttcc gttatccaac gttacgccag
     8221 ccaacgaagc catatattca cggatttgcg tcatatccgt tgcagccagc aaaccttgtt
     8281 ttgcctgcaa atcaggcagc agttgcgcca aaagcgcgcc cttaagcata tttgcctgct
     8341 ccgccgccgt cttatcgcct gcagccgcgc gtttcttata atcttggtat tgcttatcct
     8401 ttactagcat ggcatcggca agacgggaca acacctgcac tgcgttttcg ccgttttgct
     8461 tgccttgcac aaccgagcct atccaatcga cacctttctt cgggtcattc ggatttgcca
     8521 tcttcttcag acgacctatc gtgtcaggcg acagagtttt actcaaaaga ttttgcacat
     8581 tagtcgccgc ttcggcagga ctgcccgatt tattcgccgc agattgtaag agtgagagca
     8641 ggtagtccaa accgccgaca ccattcatcc ctgcctgttg cgcggcagag agcaggctcg
     8701 gcagctcccg aaccatatcc cgcacctcga aagtgccgtc taaacccgat tgcaagacgt
     8761 gctcaagccc gagttgcagg tctttaccgc tcatgccgcc atctttcagg gttttaatca
     8821 gttttgccgt atcctcgcca ctgccttctg aggcaagtgc aaaagcatat gccgcctgcg
     8881 cttcattctt ggtttgggca aaattcagac cggtggtcat catgccgctg attaaatcca
     8941 aagccttatc gtgggtcccg ccatttttct cgacaagttc aagtgccaaa tccttgatct
     9001 gttgcgcacc ttcagttgca atccacgctg ccgatttact gttatcctca ataaatgcct
     9061 gtctggacac gcggttgatg ttctcatcaa gctgctttct gttgtccata gcaggcttaa
     9121 gcaccgtata cgctgccgcg ccaccagcca ccgcagctgc accgaatctt ccgatttttc
     9181 ccatcttgtc cgcaaatccc gtgccctgtt tcagttccgc gttcagctcg cgaattcggt
     9241 tacgcgtagc gaccgccgcc cgtgccaaat cattttgtga tgccttgccg ctgcgcgcca
     9301 aacggttaaa cgcagcctgt gtcagcatga tttcgcgttg aatctgtttt tctgagcgga
     9361 tgccggtcaa cgcataagta tggctttgcc gttcaagttg cgcccgcgat ttcgcggtac
     9421 gatctaattg tcgttgagta tcagccagta gccgtctcgc tccgtcatct cgggcggtta
     9481 acaccaacga cagtttcatg ttcccgtttg ccataaaaag cagcctttaa acgtattaac
     9541 tcaaggtcaa ttatcgttta aaggctgttt aagggaggcg gtatgggctt caaccccgtt
     9601 ttcgtgcttt atttgtttgt tgcgttataa tactagaatg ataaccattt tattcaaagg
     9661 gatattttat gaatatgaaa aaattgattt ccgcaatttg tgtttcaatt gttttatcag
     9721 cctgcaacca acaatcaaaa acggcacaag ccgaagaacc tgtccaaagt atccaggctg
     9781 ctgattgtac cgccccaatg gacatcacag ttgaacaata tctcatcaat ttggagcaag
     9841 catttaaaac tcagaacgtc tcaacaaaaa tccataataa aaatattgtc aagaccgatt
     9901 gtggttatga ccttactttg gtaatggatt ttggggcgat tgcgctcaaa ctggacgagc
     9961 agcaaaaaat tagagctatc tcagtaggct acattttaaa aaccgacgga gagaaaggac
    10021 aaaatctagt caataatgcc ataaatggat tacacagtat tcaggcagtt ctgtctttaa
    10081 ctaccacaga caaattgggc gaatcggaag caggaaaaca actttttaca gctttaaccg
    10141 aagtcgtcaa agaatccaat cagacaggag caacagcgca aaaagacgtt ccggcagatg
    10201 gtattttata tagcgttgtt tttgaaaaag aaacaaacac cattgcaata atcggcagaa
    10261 aacaacccta accgtcaaat aaaaaaaagg tcgtctgaaa ctcagacgac ctttttattt
    10321 tccctccggc tttctcagcc gtttcgacac aatcactccc tcttccttcg gcgtttttat
    10381 gcccaggctc gacaggatgt cttcaaacca tgccgacagc tcggcgtggc acatatcgcg
    10441 cactgcgtcc gcccctatcc ccgccttgcc atcaggatga ccgcctgacg gtagcttttg
    10501 tgtgccgtca ggtagctgcg ttttactgct ccgccgccga cggcgcatcg gggctcgccg
    10561 tagcggcgga cggttttagg cgcaaatcct cctgagccgc catcacggta tggtagtctt
    10621 cggtagagag gttttccatc agccactgcg ccgtcaggat gtcggatggg ataccttggg
    10681 cttcaatctg ctgcgaccag taggtcagcg tctctaggac gtcgcgttgc agcagttcgg
    10741 cttttgtggc gttttcgggc agctcgggca agtcatcaat cagggcgaac gccgccagtt
    10801 cgccgccgac ggtcagtggc ttgaggcgta attcagatac gactttgcca ttgtaggtca
    10861 gcccgtgggt cagtttgacg gtaatcacgc attactcctt gattttgtgc aaagcaatca
    10921 tctcaatgtc gatgcgtgcc tcgttatcga cttcatattg ctcgccggta tcgacggtaa
    10981 agcagtcgag gtaggaagtg cggcgatctt cgtcgttgat ggggtaaatc gtgattttcg
    11041 ccttggtgat gttgtcccag tcgatttccg taccgtcgat cggaatggcg gcggtaacgc
    11101 ttaatttgtg ttcggttacg ccgtcacaat aaccgttgac tcggccgttg cggttcatcg
    11161 ttttgaccgg cttgcggcct gtagtggttt gcggcttgat gctcacaatc tcgatatcgc
    11221 ggccgttcat ctccataatc accgcgtcgg cataggtagc gtcgctcatg ctttatcctt
    11281 ttttaaggtt tcagacggcc tttaaacctg ttttaaacgc cgtctgaaaa gggttacaaa
    11341 atcaaatcaa tgcgcccggc aaagacgtgc aggccgttga ccacatcggc ggggataatg
    11401 gcattaacac ggttggggtc gttttgcgca cgcgccacca ccagcttgcc tttgttggcc
    11461 tcggcgtttt cgatgatttc ggcttggtcg agcttaatca gcacgtccaa aatctcgctc
    11521 ttaaccttgg gcagcaggcg gtcgctcaat ttgtcgcgcg gaaaacgcag ggcaatgcgc
    11581 tctttaacgc tgcggcgcac ataatccagc gtgcggatgg tggtaatgtc gagtagtgcc
    11641 gggtcgtcgg tgttgttggc cgacttggta taggtggata cggcacgcat aatctgcacg
    11701 cggttgttga ccactgtgag cggggtcaag ccgttgtaca gcgcattgtt gcattctgca
    11761 aacagcggcc attgcgcatc aggtgtaacg gccagccctt tgatttccag cgtgttcagc
    11821 ggcttggcag ggtcttcttc aaaggccaac accgccgcat aacctgcggc gatgatgccg
    11881 tttggctcta ccgcaccttt gtaccaagca caggtcatgc ggccgtcgtt gatttcgccg
    11941 gtagcggtgg tggccgtgct caaggccgca ctcataccca atacgccgat acagccgcgc
    12001 tgctcgatgg cgttggatac gttggtaata tggttgctca aggctttggc gttggcgtca
    12061 tcgctaaacg ggctgcaaat aatgtgataa tgcttgcccg ccactttgga caaggccgtg
    12121 gcaatgtccg catttttggc accgccggta aaggcattgg cttgataagt taggccggta
    12181 ttgccggtgc tcacggttaa gccgctctcg ttgccgatct cgcctttgtg tttggccgtc
    12241 agcgtaacgg ctgcgccgct gccggatgcg gtaacggtta catcggcggc agtgatggcg
    12301 gttttcagac ggtctgccac tgtggcggcg gtctcgccgg tgttaacggc cgtgcttacc
    12361 tgcttgccgc caatcgtgat ttccaccacg cccggcgcgg tggccgtgcc ggaaagggta
    12421 acggttgcgg ttgcctgcac gcctgcgctg tggtcggcaa taccgataac ggtcaaatcc
    12481 aaataagggt tgttggcaaa tgcttggcgc accatcaaat gcgccagcga gccttgtccg
    12541 aacaaatcgg ccgcctcggc atcgctaaat agttgcaccg gctctaaggc gggctgtatg
    12601 cccgcggtca gcatgggtgc aaccatcaat accttttgcg gattttgcgg caaaccttgt
    12661 acggcattgc gggtgttaaa ttcaatatac tgcccgggca cgcggatgct gcccggaatc
    12721 gtgtcaaaat caatatgggg catcattact ctcccttagt attgcgggtt ttggtatttg
    12781 gggcggcatc ctcaaccacc accaaatcgc cgtcatcaat catgcggcgg taatacaggc
    12841 tgttgccgtc cacctccacc ggctcttggc cgatatattc gtgcgggttg tgttcggtcg
    12901 gcacacgcag gccatctgcc gccgttactt tgattttgct cattgcttat ccttaagggt
    12961 taggtttaca ggtatttcat cggcggattg cgggtcgtaa attttgccgt ccaacccctc
    13021 gaaatcaggc cacggctcgc tcaatgtacc ctgatacttg gtaaagatat ggttggggtc
    13081 gtcgggattg tcggtgcgtt cggggtagcg gtcattttcc aacccgcagg tgttaaagcg
    13141 gatggcatac tcaaccgcat atatgcttac tgcggcgttt tgcaccagca catgattggc
    13201 aatcgcgcgc accgctttgg gcaccaagcc gcggctatcg gcaaaaccga gccgctggcc
    13261 gtcaagcagg cggcgaacag cgcggattaa atcgttgctg ccgatttcgc ggctgtcgat
    13321 gccgccttgc cgctgcgcct gctcgttgcg cagattgcgg gccgccacca tcaccacaaa
    13381 ttcggcggta tcctgataac gtccgcatac gccgccggtg ctggcaggct caactttgct
    13441 gccgccatac gttacccaaa ccgcaggcag cgtatggatt tgccccgcca aatcgtcggc
    13501 ctcgccgttg tagcttttaa ccgtgcgcac catgcgaccc aagccccgtt tcagacggtc
    13561 tgttatcgct tgctcaatat cagtgatcac gcccgaacac cttattttta ccgtttacaa
    13621 acacaatacc gtcttcgccc gcggccactt cttcgccgct atcatccacg cccagctgca
    13681 cttcgccttt ggccatagca cgcagcaggt cgagcacgtc gattttgtag cggttgcgga
    13741 tttcgtcggt aatcaacacg ccctgagccg ccgtcagacg gtagcgggca atgtcgcagc
    13801 aaaggcgcac caagatgggc ggcagatcct caaaaggtcg tctgaaccgc cccagatacg
    13861 cgtcgatttc ggcagtggcg tccaccagcg cggtttgtgc gacctcgcgg tcaatcagcc
    13921 cctcgttgtt gcggtcggtg agctgcaaga cttccagctc accgaaacgc gcaaccatat
    13981 cctcaaccgt cgcgtatgcc attactcgac cgccttgcgt tgcagcatag gctcggcgca
    14041 gattgccttc cacaccgctt cgccgacttc ggcgcgcttc acttcgcgcc agccgccgtc
    14101 aaacagcagg ccgccgcgcc aaaattcttt gccgtctgcg ccggtactga cgagcatcac
    14161 atcgcggctg tccgccaaag cgtcggcggc acgttgcgta tgctgcactt tgagttcggc
    14221 aagttcggcg gacagtgcct ttttgtcgtc ttcggctttt tccaaggctg tggtcagcat
    14281 ttcgacatcg tttcgggcgg cggcaagctc tgcctgcacg gcgtccaatt cggctttgat
    14341 gtcttcaaac gacggggcgg cggtttcggc ggtttctggt ttgttgttgg tttttgccat
    14401 gatgactcct tgtt
//
LOCUS       DAWGRZ010000038        14009 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000038 DAWGRZ010000000
VERSION     DAWGRZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14009
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.038"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            52..1005
                     /locus_tag="WHT44_000789"
     CDS             52..1005
                     /locus_tag="WHT44_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ2533002.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHNVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1116..1439)
                     /locus_tag="WHT44_000790"
     CDS             complement(1116..1439)
                     /locus_tag="WHT44_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533003.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGGN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1612..2973)
                     /locus_tag="WHT44_000791"
     CDS             complement(1612..2973)
                     /locus_tag="WHT44_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ2533004.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGTGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAETIEVTTFRGGAKVHQNARGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAAHRLVMIGDAAERYQEDPVRILRAIRLSGKLGFELSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHLLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
     gene            complement(3270..4970)
                     /gene="recJ"
                     /locus_tag="WHT44_000792"
     CDS             complement(3270..4970)
                     /gene="recJ"
                     /locus_tag="WHT44_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ2533005.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIAQLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAARRLADAVGRKEKILIVADYDADGATACAVGMSGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLMALRAELRRRNYFSDGIKEP
                     NLGELLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAAQLNNLNIERREIEQSM
                     LQDALNDFPETLPSGQMTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTTFEEAVRE
                     MVCEDDLSQTFITDGSLPACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
     gene            complement(5039..5755)
                     /locus_tag="WHT44_000793"
     CDS             complement(5039..5755)
                     /locus_tag="WHT44_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533006.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTLMLWGMNNPVSETITRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTEGLCLRYDTDKPADIAKLKQLGFEAVKLDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEEHTDKSKLFANILYTPPFLILDAA
                     GAVLALPAAALGAVVDAARK"
     gene            complement(5802..6314)
                     /locus_tag="WHT44_000794"
     CDS             complement(5802..6314)
                     /locus_tag="WHT44_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ2533007.1"
                     /translation="MKMFEQEDEWISKTQMKKQMNDLQDLGMALTKLSNDTLKKIGLD
                     ADLYEAVTAYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQR
                     VEQARVRLLADDGALTQFMSDFPHADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELK
                     TVMENGDAEI"
     gene            6461..7792
                     /gene="pmbA"
                     /locus_tag="WHT44_000795"
     CDS             6461..7792
                     /gene="pmbA"
                     /locus_tag="WHT44_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ2533008.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKATGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRASTADFSEKALQDTVKAAIDIARHTAEDGCA
                     GLADACLMAKHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(8089..8292)
                     /locus_tag="WHT44_000796"
     CDS             complement(8089..8292)
                     /locus_tag="WHT44_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ2533009.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8535..8837
                     /gene="clpS"
                     /locus_tag="WHT44_000797"
     CDS             8535..8837
                     /gene="clpS"
                     /locus_tag="WHT44_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ2533010.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8839..11154
                     /gene="clpA"
                     /locus_tag="WHT44_000798"
     CDS             8839..11154
                     /gene="clpA"
                     /locus_tag="WHT44_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ2533011.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVHNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDEDEDEDEDDG
                     NYSSDGMDDDNGNRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRR
                     RKNNPLLVGEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDF
                     EARVKSVLKQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGA
                     TTYDEYRTIFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALE
                     AAAELSARYINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIP
                     EKTVSHDDKQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSG
                     PTGVGKTEVAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAI
                     NKQPHCVLLLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAES
                     LSRPSLGFTAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPVITKVVDKFLLQL
                     EHRLLDKKVEAEFTSALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSD
                     GGFVRIDWDAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(11193..13943)
                     /locus_tag="WHT44_000799"
                     /pseudo
     CDS             complement(11193..13943)
                     /locus_tag="WHT44_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT         3256 a         3997 c         3507 g         3249 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgccaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgc
      361 accaagcgcg gcagcatcgg cggcagaacg ccacggcaaa acggctatat ccgcgccctg
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgcc
      481 gttgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgc
      541 ccagccgtcg aagccggcga gaaactgggc ttcctgcccg gagacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt tgaccgtgta
      661 accaagctga ttgaaaaagg cctgattgaa atcgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 aacgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctttg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcaggcaga gaaaaacaga tacggaagcc aagctccgta
     1081 tccgtatccg tttctttatc cgtccaaccg atacatcaat cgaattttct gcatcggatt
     1141 gcctcatctt cgcaaaacat tgccgaagtt tcgcggttgc acgcctgacg cactttctgc
     1201 cgcgccacgc cttcattccg atcgcttgcc tcaaacgtct tctgaaaagg cgtaacgctg
     1261 catacataca tggtggaatt tgaaccgaaa aggtcgattt tgacggtatt gccgccgctt
     1321 tccaatacgg cgacacggtg ttccaactgc gccagacgtg cttccaaatc gccgattctg
     1381 tcggcaaatg caggcatggc ggcacacgcg gtcaaaatgc ccaaaataca ttttttcatc
     1441 aatttttcct tgttgcttga accctgccca cccggacatc ctttttccga gaggcagggt
     1501 tagactttat ttacggggat gaatcccctt acaaatcagg ataatgcata gaacggcact
     1561 ttatgcttca tcccatgtgt tgaaacttca ttgctccatg tctgttgacc cttattccca
     1621 atccgttccc acaaccttcg gcttgggttt gcgcctgcga cggcggcgtt ttttcgcctg
     1681 tccttcgttt ttttcgtgtc gggcggcggc ttcgtttttg gtcatctccg accgctgttc
     1741 cgtcgatgcc gtctgaaacg ccgtccacca ctcggcaagg gcgcggtccg cattgccggt
     1801 ttcggcgcgc aagagcagga aatcataggc ggcacggaaa cgcgcctgtg caaacagttt
     1861 gtgcggcctt gcgcctttgc ggttttcaaa ctgcggctgg aacatccaaa tttcgcgcat
     1921 cgtggcggaa aagcgttgcg gcacgcccca accgcgttcg acggtttcgc gcatcgtatt
     1981 gattgcatcg gacagggcgg gcgcgggttt caaaccctgt tgcagattgc ttttccaatg
     2041 gcgttccaac tcgggccaca tcagagccgc caatacaaaa ccgaccgaaa ccgatttgtc
     2101 ggcacgcagc cgctcatcgg tatttttcag ggcaagcacc gtcatttttc cggcgatgcc
     2161 gtctgaaacg cgcaaggcat tgagcagcag atggatgtcg tccggtatgt caaatccgtt
     2221 caaacgtttt agacactcgc gagcgtgccc tgaaaacagc aatttcataa tttcgtcgaa
     2281 cagcctcgct accggttcgt gcttcagacg gcatatcgat tcggcaatcg gtgcggcggt
     2341 ttcttccgac agctcaaagc ccaatttgcc cgacaggcgg atggcgcgca aaatcctgac
     2401 agggtcttcc tgatagcgtt cggcggcatc gccaatcata accagcctgt gggcggcaac
     2461 atcggcaatc ccgttgtgga aatccaaaat ctcttctttt tcaggatcgt aatacaaggc
     2521 attgcaggta aaatcgcgcc gcatcgcatc ttcttcgatg ctgccatagg tattgtcttt
     2581 cataatcctg ccgcgtgcat tctgatgtac tttcgcaccg ccgcgaaacg tcgttacttc
     2641 gatagtctct gcaccgttca tcacatggac aatctgaaaa cgcctgccga tgatgcggct
     2701 gcggcggaag agtttgtgca cctgttcggg cgtggcatcg gttgcgacat cgaaatcttt
     2761 gggttcgatg ccgagcagca ggtccctgac cgcgccgccg accacataag cctgaaaccc
     2821 cgtccctttc aggcggcgta tgacgttttc ggcggcaaag ctcaacattt cggcacggat
     2881 gttgtgtctt tcggcaggaa tgaccgtttt actttccgct tttttactgc tccgaccgga
     2941 aggcagcatc ttattcaacc attttttcag cataaatatt ctgacccgaa gagcggcatc
     3001 tccacaggca accgtgttca gacggcatca ggcgcggttt gcaagatgcc gtaaccataa
     3061 acaaaaggcg tagaaactac gccgtccgaa tcacgccgcc ctcgcggcgg caacgcgtca
     3121 ttataacaga ttgccctcca aaaaaatcag accctgcttt tgtggcagag tctgaaattt
     3181 gatgcgcctt ctccgattgg gaacattccc gtcgtacaaa caggcaaatg aaggaacaga
     3241 aatcacattc aaacagtgtt ccgccgcctc tacgcggctt cccagtaatc gatatacagc
     3301 tgcaattcga gattgttgcg ccattcgttg gcaacggggc ggtaaaccgt gcggatgtat
     3361 tcgggaatgt cttcgctgca acgccaaaac atcgcttcaa attcgcagcc gtctttttgc
     3421 agccagactt ttttgtgttt gccctccgcg cccaaaggtt gctggcggac gacgtggaac
     3481 tcgtcggtaa agctcggcgg cgcgaagccc tgcccccaaa cgtgacgggc aaggttttgc
     3541 gcctgttcca acgtgatgtc gcaggcgggc aggctgccgt cggtgatgaa ggtttgcgac
     3601 aaatcgtctt cgcacaccat ttcgcgcacg gcttcttcaa aggtcgtctg aaacgcggga
     3661 atgttgtgtt caagtatgct caaacccgcc gccatcgcgt gtccgccgaa tttcaaaatc
     3721 aaatcgggat ggcgtttgga caccaagtcc aaagcatcgc gcaggtgcaa attgggaatg
     3781 gaacgtcccg aaccgcgtac ttcgccgttg tcggcaggcg caaacacgat ggtcggacga
     3841 taaaaacggt ctttgaggcg gctggcgaca atgccgacca caccttgatg gaagtcgtcg
     3901 cgatacgcca ccaaagtcat ctgacctgaa ggcagggttt cggggaaatc attcagtgcg
     3961 tcttgcagca tagactgctc gatttcgcgg cgctcgatat tgaggttgtt taactgagcc
     4021 gccagttcct gagcttcgga atcatctcgc gccaacaggc aggcgatgcc gaccgacata
     4081 tcgtccagcc gtccggcggc gttgatgcgc gggcccaacg caaaacccat atcaaacggc
     4141 tgcgccttgc gccaatcgcg ccgcgccact tcaaacaagg cgcggatacc ggggcgcatt
     4201 ttgccggagc gcatccgttt caaaccttgc gacacgagga tgcggttgtt gtggtcgaga
     4261 gggacgacat cggcaaccgt gccgagtgcg accaaatcca aaagttcgcc caaattcggc
     4321 tctttgatgc cgtctgaaaa ataattgcgg cggcgcaatt cggcacgcaa cgccatcaat
     4381 acataaaaaa tcacgcccac gcccgccaag cttttgcttg gaaaaccgca gcctttttgg
     4441 ttcggattga cgatgatgca gtcgggcacg gtctcggccg gcaagtggtg gtcagtaacg
     4501 atgacatcca aacccagagc ctgcgccctc gccacgcctg cgatgctggc gataccgtta
     4561 tccaccgtaa tcagcaaatc cacgccctgc gcggcagcga tttcggcaag ttcgggcgtt
     4621 aagccgtagc cgtgttcaaa gcggttgggc acaaggaaat ccactttcgc ccccatcgcc
     4681 gccaaaccgc tcataccgac ggcacacgcc gtcgcaccgt cggcatcata gtcggcaaca
     4741 atcaggattt tttccttgcg cccaaccgca tccgccaaac ggcgggcggc ggcttcgcaa
     4801 ttcgtcaacg attgataagg caggagggaa gcgagtttgt cgtccaattc ggcaggactt
     4861 tgcacaccgc gcgaagcaca aagctgggcg attaaaggat cggcaccggc ggtgagcaaa
     4921 tgattaaaaa cgtcggtatt gacggatcgg gtttggattt tgactgacat aacgtggggt
     4981 tttactgtgc gtgtgtgctt aaaatgccgt ctgaaagctt ttcagacggc attgctgttc
     5041 atttgcgggc ggcatccacg accgcaccca gagccgccgc aggcaaggcc agtaccgcgc
     5101 ccgccgcatc cagtatcaaa aaggggggcg tatataagat atttgcaaac agcttggatt
     5161 tgtcggtatg ttcttcagta accgtgtaat aaatatcggc aggcacactt tgctcaaaat
     5221 ggtaatcggc gttcagtttt tgcggtgtgg cgtagtattt gcctttggcg gatacgcagc
     5281 gcgtgtaaat ggtccgattg tcgagtttga ccgcttcaaa cccgagctgt ttcagcttgg
     5341 cgatgtcggc aggcttgtcg gtatcgtagc gcaggcaaag gccttcggta ctgaaattct
     5401 ggctgccagg cgattcgagt ttgaccggca gggcttggtg gcgagcatag ctcggggtat
     5461 cctcaactat ttggaagggt ttgtccagcc ctgccttcaa aatgcccgtc agcttcgccg
     5521 aatcttcggg attgacgacg aaccagtatt ttccgcccat catcaccagg ctgccctttt
     5581 ccaattgggc attgtcttcg gcaaccacac cgaaggcgcg gatttggtct ttgtcaacgt
     5641 gtttgcgggt gattgtttcg ctgaccgggt tgttcattcc ccacaacatc aacgtacagc
     5701 cgttcagcat caaggttgcc gccaaaacgg cggcggtcgt tttacggaac aacattttat
     5761 cctccaatct aaataacggc ggatgtcgtc tgaaaatatg cctaaatttc cgcgtccccg
     5821 ttttccatca cggttttcaa ctcttgaaac agggcgcgga aattttttgg tggtttgttt
     5881 tgctcctgct cttttttggt attgcggatg agtgtcctca gcttgcccgc gtccgcatgc
     5941 ggaaaatccg acataaactg cgtcaacgcg ccgtcgtctg ccaacagccg tacgcgcgcc
     6001 tgttccacgc gttgcaaaaa ggcgttgtgc gccgcatcgt cgccgcgcag cttggcaaga
     6061 aacgcctcga tgggcgcggg atcggtatcg cgcatcagcc tgccgataaa ttgtgcctgg
     6121 cgtttgagcg cgccgttgga tgtgattttt ttataggcgg ttaccgcctc gtacaaatcc
     6181 gcatccaaac cgattttttt cagcgtatcg tttgagagct tggtcaacgc catacccaaa
     6241 tcctgcaaat cgttcatctg ctttttcatt tgggttttgc tgatccattc gtcttcttgt
     6301 tcaaacatct tgattctcga atttttccaa tagcgcattt taacagaatg ccgccgcgtt
     6361 cagacggcat tttgcaccgt ccgccctgca aacggcgcgc ttttgcccct gccgcccgaa
     6421 acggttaaaa tgccgcaaag catcatcaag aaagatttct atgctgttca accacaccgc
     6481 ttccgaactc ctcgacctct gccgccgcac gctcgacttg gcaaaagcga cgggcgcaac
     6541 cgccgccgaa gccgatttca gcgaatcatt gggacaaagc gtcagcgtgc ggctgggcga
     6601 aatcgaacaa atcgagttcc agcaggacaa gtcgctggac attaccgttt acgtcggcaa
     6661 acgcaaaggc cgcgccagta ctgccgactt ctccgaaaaa gccctgcaag acaccgtcaa
     6721 agccgccatc gacatcgccc gccacaccgc cgaagacggt tgcgccggac ttgccgatgc
     6781 ctgtctgatg gcaaaacaca tcggcgaccc cgacctttac cacgaatggg atttggatac
     6841 ggaagccgcc gtcggcttgg caaaacaatg cgaacaagcc gccctgaacg aggatgagcg
     6901 catcgaaaac tccgaaggcg cggcggtgca aaccggccat taccaatacg tttacggcaa
     6961 cacccacggt tttgccgcac accggcaagg cacgcaccac agcatttcct gcagcgtcgt
     7021 tgccgccgac gaaaacggca tgcagcgcga ctactggtac gattccgcct gccgccatcc
     7081 ggatatggac agccccgaaa ccatcggtca aaccgccgcc cgccgcactt tgcgccgact
     7141 cggcagccgc agcatcccga caggcagcta ccccgtcctc ttcgatacca ccgtttcggg
     7201 cggtcttatc ggacacctcg tcggcgcact ctccggcggc gcgctctacc gccaaagcag
     7261 tttcctgatc gacagcatcg gcaaaaaagt cctgcccgat ttcctcaacc tgcgcgaaga
     7321 accgcacatc ccccgctctt tccgcagcag ctattttgat gcggaaggcg ttgccaccgc
     7381 accgcgcttc gtgattcaaa acggcattgt cgaaggctat ttcctcagca gttacagcgc
     7441 gcgcaaactc ggtatgcaga ccacgggcaa cgccggcggc gcgcacaacc tgtatttgaa
     7501 ccatacgcac gaaacgcaat ccgacctgct gaaagaaatg gggacgggat tgttggtaac
     7561 cgaactgatg ggacagggcg cgaacaccat taccggtgac tactcgcgcg gcgcggcggg
     7621 tttttgggtg gaaaacggcg tgattgccta ccccgtccac gagattaccg tagccggacg
     7681 cttgcaggat atgtaccgcg acattgtcgg cgtggcggat gacgctttgc ggcgttcgtc
     7741 caacaaaatc ggctcgattc tgattgcggg gatgacggtt gccggaagct gaacgccctg
     7801 ccgatgccgt ctgaagacga aggcagcacg ttttgccccg tgcctacccg tttgccgttt
     7861 tatgccgcac cgccttttgc cgacaacatt gcacattttt tcatcagcac tgattttccc
     7921 ttatccgtca aaatactaag ccacttgtcc acaacagggt acacacaagt tcaggataag
     7981 gcgtgcgggg attggggttt ttccgccgcc ggcaaaacgg cagacaaaaa cggcgggaca
     8041 ttgcctgtcc cgccgttttc aaatatacat ttgaccgtac atcaggaatt aagcagcctg
     8101 aatgttggcg gcctgtttgc ctttagggcc ggtggttacg tcgaaagaga cgcgttggcc
     8161 ttctttcagg gttttgaaac cttccatatt gattgctgag aagtgagcga acaaatcttc
     8221 gccgccttca tcaggcgtga tgaaaccaaa acctttagcg tcgttaaacc attttacgat
     8281 accggttgcc attagaaact tcctatactc aaaaattaac aaaatcagca aaacaaggca
     8341 tacggcaaac cgagccgtct ggcgttcctt ccagcttgat atactcggcg gatgcccaat
     8401 cctgcttaac cgtcttttta catttgttaa gacaaaacgt caagctatgt tttcaaaaaa
     8461 gtgggaaaat aggcaaaagt accgaaaata caacaaaaat gccgtctgaa cgcggctttc
     8521 aaatgggaca cactatgacc gcgcaacacc aatccgacac gctcttgcac cgtctgaaca
     8581 ccctgccgcc gaaacgttac ggcgttttcc tattgaacga cgattacacc acgatggaat
     8641 ttgtcgtcga aatcctgacc gaaatcttta tgctcggaca agaacaggcg gtagcggtaa
     8701 tgctcttggt tcatcacgaa ggcaaaggcc tgtgcggcac ttacacgcgc gatattgccc
     8761 aaaccaaaca acaacaagtc atgcagcggg caaaagccga agggcatccg ctgcaatgta
     8821 ttgtcgagga gatttaatat gcttgcaccc gaattggaac agattttgca gcagctttac
     8881 cgcgaggcgc gtaaggctca ttatgaattt atcagcctcg agcatctgct tttggtactc
     8941 atcgaagaag atgcctccgt ccacaacgtc ctcaagctct gcggcgcgga tttgaaagtg
     9001 gtgtccgaac agctcgccgc cagcgttgcc gaaaacaccc ccctgattcc cgaacacctt
     9061 ttagacacgg tcgaaacccg gcccacgctc ggcttccaac gcgtgatgca acgggcgatg
     9121 gtgcataccc agtctgccgg aaaagccgca gtcgaaccgt tggacgtttt ggtcgcgctg
     9181 atgagcgaaa ccgacagcca caccgtctat ttcctcaagc tgcaatcggt tacgcgtttt
     9241 gaagttttgc gctgtattgc ccacggctct cccgatgaag atgaagatga agatgaagat
     9301 gaagatgaag acgatggcaa ctattcttca gacggcatgg acgacgataa tggaaaccgc
     9361 accaaaccgg gcaaaaaccc tttatcggcg tacaccgtca acctcaacgc cgaagtcaaa
     9421 gccggccgta tcgacccttt gattggtcgc aaacacgaaa tggaacggct ggtgcaaatc
     9481 ctatgccgcc gccgcaaaaa caatccgctt ttggtcggcg aagcgggcgt gggcaaaacc
     9541 gcgctggcgg aaggtttggc acatcaaatc gtcaacggcg gcattccaga cgcgcttaaa
     9601 gatgccgaag tgtacgcgct ggatatgggc tcgctgttgg cgggcacgaa ataccgcggc
     9661 gactttgaag cgcgggtcaa atccgtcttg aaacagctcg aaaaaatccc gcacgccatt
     9721 ttgtttatcg acgaaatcca caccatcatc ggcgcgggca gcaccagcgg cggcaccatg
     9781 gacgcgtcca acctgctcaa acccgcgctg gcaaaaggtt cgctgcgctg catcggcgcg
     9841 accacctacg acgaataccg caccattttc gacaaagacc atgccttaag ccgccgcttc
     9901 caaaaaatcg acgtggtcga acccaccgtt tccgaaaccg ttcaaatcct gcgcggcttg
     9961 aaaccgatgt ttgaagcctt ccaccaagtc cgctacactc aaggcgcact cgaagccgcc
    10021 gccgaactct ccgcacgcta catcaacgag cgtttcctgc ccgacaaagc catcgacgtg
    10081 atggacgaag caggcgcggc gcaacggatt ctgcccaaat ccaaacagaa aaaagtcatc
    10141 ggcaaagcgc aaatcgaaac cgtcatcgcc aaagtcgcgc ggattccaga aaaaaccgtg
    10201 tcgcacgacg acaaacaggt gctgcaattc ctcggccgcg atttgaaaaa catggtttac
    10261 ggtcaggaaa acgccatcga cgcgttggtt gctgccgtca aaatgtcgcg ttccggcctt
    10321 gccctgcccg acaaaccgat aggcagtttc ctcttctccg gtccgactgg cgtcggcaaa
    10381 accgaagtcg ccaaacagct tgcctactcg atgggcgtac cgctgcaacg ctttgatatg
    10441 tccgaatata tggaacgcca cgccgtatcg cgcctcatcg gcgcaccacc gggctacgtc
    10501 ggctttgaac aaggcggcct tttgaccgaa gccatcaaca aacagccgca ttgcgtattg
    10561 ctcttggacg aaatcgaaaa agcccacccc gacattttca acgtcctcct gcaagtcatg
    10621 gacgcaggca aactgaccga caacaacggc aagagtgccg atttccgcaa cgtcatccta
    10681 attatgacca ctaacgcagg tgcggagagt ctcagccgac ccagcctcgg ctttaccgcc
    10741 aaacgcgagc gcggcgacga aatgcaggct atcaacaagc tcttcacgcc cgagttccgc
    10801 aaccgcttgg atgcgattat cccgtttgcg cccttatccg aacccgtcat caccaaagtc
    10861 gtggacaaat tcctgctcca gctcgaacac cggctcctcg acaaaaaagt cgaagccgaa
    10921 ttcacgtcgg cattgcacaa atatctggcg gaaaaaggtt ttgacccgca aatgggcgcg
    10981 cgcccgatgc accgcctgat tcaggaaaaa atccgcaaac cgctcgccga cgaactcctg
    11041 ttcggcaaac tatccgacgg cggcttcgta cggatagact gggatgcggc aaaagaagaa
    11101 gccgtgttga agtttaagaa aagcaaggtc aaaataaaaa ccgcctccgc ataaaatcaa
    11161 aaatgccgtc tgaaatttca gacggcattt tgtcaaatat cggtccctat tccggcaaac
    11221 ttctccacaa acgccgcaat cttttgaaaa acactttgtt tcttcgtcag atattgcggg
    11281 ttgagcggac tcattttcgg cagggtttcg gtcagttccg tgccgttttc gctggcatag
    11341 ccgcgtttga gcgagctgat taaatagcgt ttggcggcgg tttcgttcag gccttcggcg
    11401 gcaatcaatc ctgccgcttc gtgccgcatc acctcttgcg cgtaggtgta gaaggtttcc
    11461 agtatggcgg gaacgtcggg tactttgtcc aaatccgtat cgttgatgaa atccacaatc
    11521 agaccctctt tggcgcggtg gccgatgctg gcgcggatga tgcggcggat ttcttccacc
    11581 agctccgctt tgcctttgat ttttttgtgg tgttcgaaaa ccagttgcag gatgtaatcc
    11641 agattgattt cctgtgattt gagcaaatcc acctcaaaaa ccacatcgtc ccagtcgatt
    11701 tttgattgct ctttctgttc gcctgctttt tggcggcgca gccagtcgcg gatgtcattg
    11761 taggcggaac ggtagtcctg caccgccctt tcagacggca tcggcacttg ccgcatttcc
    11821 tgcacgtctt catcgctcag gtagtatttt tcttgaaacg ccttcatcgc atcttcgtcc
    11881 gccgcgtcca cactctgcaa ctcgcgcagc gcggcaaatt catcgtagtt ctgcaatacg
    11941 ttttccgccc gcaggtattc gccgaagagt ttggcaaaat cttttttgtc tttttccgtt
    12001 tcgattttgt cgggatcggg gaaacgctcg cgcaattctt ttgccacatc cagataaccg
    12061 cgccgtgctt cgccggtctg gctgtcggta tagccgttca tgtattcttc gtaacttttt
    12121 tccagcacca cgtttttggt gtttttgtcg ccaaacaagg taatcgcatc aatggttgcc
    12181 tgctccaaat cgcggaagca gacaatattg ccgaaggttt tggtggcatc gtaaatgcgg
    12241 ttggtgcgcg aaaacgcctg catcaggccg tgatagcgca ggtttttatc gacgaacaac
    12301 gtgttcagcg tcggcgcgtc aaaacccgtc aaaaacatgc cgaccacaat cagcaaatct
    12361 atttcctgat ttttcacccg ttttgccaaa tctcggtagt agttttgaaa ggctttgctg
    12421 tccgtgccga aattggtttt gaaacaggcg ttgtaatcgt tgatggcagc ctgcaaaaat
    12481 tcttttgcgc tgctgtccat cgcttccggt tcaaaagtct catcgacaat ttcaccgacg
    12541 gcgttttgct cttcgttggc cgcaaaggaa aaaatggtgg ccactttcag cgggtgcaag
    12601 ctgcctgcct gttgtgtttt gaacgcttcg taatagcact tcgccgcatc cacgctgctg
    12661 acggcaaaca tcgcgttaaa gcctttgcca cccgcattca gccggtgcgt tttctgcctg
    12721 aactgattca ggatatattg cgtgatttcg cggatgcgtt cagggtgcag cagggctttg
    12781 tggttttcgg cggcactcag tttcttctcg tcctgttccg cttccacggc tttgaactgc
    12841 gggcgcacgt cgttgtaatc cactttgaat ttcaatactt tttcatcgcg gatggcatcg
    12901 gtaatcacat aagaatgcag ctcccgcccg aacacgcccg ccgtggtttc cgcgcccaaa
    12961 gcgttttcgg gaaaaatcgg cgtgccggta aagccgaact ggcagaattt tttaaatttc
    13021 tttttcaggt ttttttgcgc ttcgccgaat tgcgagcggt ggcattcgtc gaaaataaag
    13081 acaactcgct gatggtaaac cggcagatta tcttcgccct tcatcaggtt gttcagcttt
    13141 tggatggtgg taacgatgat tttgttgtcg tctttttcca aattgcgttt caagcctgcc
    13201 gtgctttccg aaccgttcac gctgtcgggc gaaaaacgtt ggtattcctt catcgtctga
    13261 tagtccaaat ccttcctgtc caccacgaag aaaaccttgt cgataaatgc cgattccgtc
    13321 gccagacgcg ccgccttaaa gctggtcagc gttttgccgc tgcccgtggt gtgccagaca
    13381 tagccgccgc tttccggttt gctccaattc ttcgcctgcg ccgagctgtt gattttccac
    13441 aaaatgcgtt cggcggcggc aatctgatac ggccgcataa tcagcagcgt atcattcgca
    13501 tcgaacacgc tgtaatgcag caaaacgccc agcaatacgc ttttctgcag gaacgtggcg
    13561 gtaaagtctt tcaaatcctt aatcggatga ttgtccgacc gcgcccaatt catcgtgaaa
    13621 tcgaagctgt ttttgtcgcg cttggtggtg ttggcgaaat agcgcgtgtc cgtgccgttg
    13681 gaaatcacga agatttgcag gaatttgaac agcgaatttt cgctgttgaa gctctctttg
    13741 ctgtaacggt gcacctgatt gaatgcctcg cgcaccgcca caccgcgctt tttcaattca
    13801 atctgcacca gcggcaggcc gtttaccaac acggtaacgt catagcggtt tgcatgcgtg
    13861 cccgtctgct caaactggtt gataacctgc acatggttgc gggcaaggtt tttcttgtcc
    13921 agcagataaa tgtttttcag acgacggcgg attcgcattt gaagtgcaac tttccctaac
    13981 agaaaaaggc cagtatgcgg tagcatacg
//
LOCUS       DAWGRZ010000039        13920 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000039 DAWGRZ010000000
VERSION     DAWGRZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13920
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.039"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(122..1447)
                     /locus_tag="WHT44_000800"
     CDS             complement(122..1447)
                     /locus_tag="WHT44_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ2533012.1"
                     /translation="MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELAGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            1658..2497
                     /locus_tag="WHT44_000801"
     CDS             1658..2497
                     /locus_tag="WHT44_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ2533013.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGETLVWLLYLIPVITWFLCGSRPSETLTRKE
                     ELK"
     gene            2494..3660
                     /locus_tag="WHT44_000802"
     CDS             2494..3660
                     /locus_tag="WHT44_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ2533014.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            3924..5189
                     /gene="efeB"
                     /locus_tag="WHT44_000803"
     CDS             3924..5189
                     /gene="efeB"
                     /locus_tag="WHT44_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ2533015.1"
                     /translation="MSKKQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGDITPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(5261..5590)
                     /locus_tag="WHT44_000804"
     CDS             complement(5261..5590)
                     /locus_tag="WHT44_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ2533016.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAVLQNGDTVILIKDLKVKGSSMVIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(5665..7035)
                     /gene="glmU"
                     /locus_tag="WHT44_000805"
     CDS             complement(5665..7035)
                     /gene="glmU"
                     /locus_tag="WHT44_000805"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ2533017.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHRIGGKPMVGRVIDTAA
                     ALNPQNICVVIGHGKEQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLSAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPNDPTGLGRIIRDSNGSVTAIVEEKDADAV
                     QKAVKEINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SHLAAGVNNKLQLTELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     CIFEGDIELGDNVEIGANCVIKNAKIGANSKIAPFSHLESCEVGENNRIGPYARLRPQ
                     ARLADDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGCKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNVEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(7090..7362)
                     /locus_tag="WHT44_000806"
     CDS             complement(7090..7362)
                     /locus_tag="WHT44_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ2533018.1"
                     /translation="MRKTFLFLTAAAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(7355..8068)
                     /locus_tag="WHT44_000807"
     CDS             complement(7355..8068)
                     /locus_tag="WHT44_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ2533019.1"
                     /translation="MQENPTVWLFDLDNTLHDADAGIFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMPETQNTLSR
                     LKGRKAVFSNGPSFYVRAVVNALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKSHALPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTPYPPKIRKKSHA"
     gene            8127..9128
                     /locus_tag="WHT44_000808"
     CDS             8127..9128
                     /locus_tag="WHT44_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ2533020.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEESAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPELAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            9222..10070
                     /locus_tag="WHT44_000809"
     CDS             9222..10070
                     /locus_tag="WHT44_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ2533021.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            10096..10581
                     /locus_tag="WHT44_000810"
     CDS             10096..10581
                     /locus_tag="WHT44_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ2533022.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(10683..12251)
                     /gene="msrAB"
                     /locus_tag="WHT44_000811"
     CDS             complement(10683..12251)
                     /gene="msrAB"
                     /locus_tag="WHT44_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ2533023.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKGKVK"
     gene            12397..13662
                     /gene="ftsY"
                     /locus_tag="WHT44_000812"
     CDS             12397..13662
                     /gene="ftsY"
                     /locus_tag="WHT44_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ2533024.1"
                     /translation="MFSFFRRKKKQETPALEEAQIQETAAKAESELAQIVENIKEDAE
                     SLAESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAVAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         2969 a         3550 c         4199 g         3202 t
ORIGIN      
        1 aatttgatcc actataattc cgtcaaataa gaaaggaatt ttgtgcctgc ggtatcgcaa
       61 aacttcgcct taatgcgccc gattgcctag ggatgggctt cagatggcat tgttttccgg
      121 tttacgggcg gtattcgggc ttcataccgt tgggtaggag ctgccagaca tatcccgtgg
      181 ttttctgttt gccggcaagt tcgccggctt cgtcgccgta tccccaaaaa taatccacgc
      241 gcaccgcgcc tttaatcgcg ctgccggtat cctgcgccat aatcaggcgg ttgagggctt
      301 tgcgggtaac cggatgggcg gtggcgacaa ataagggcgc acccaaggta atgtagtgcc
      361 ggtcgactgc gccggcatat tcccccatca gcggcgtgcc cagtgcgccg acagggccgt
      421 cattgctgct tccggcaagc tcgcggaaaa agatatagct ggggttttga cccaaaactt
      481 cggcgaggcg ttgcggattt tgccgcatat aagacttaat gccctgcatg gaggtttgtc
      541 cgagtttgag gtagccctta tccgccatat agcgtccgat ggaaacgtag ggatgttcgt
      601 ttttgtcggc atagccgatg cggatgtatt tgccggacgg ggttttcaga cggcccgagc
      661 cttggatgtg cataaaaaaa agttcgacag ggtcttcggc gtaaccgagt atcggggctt
      721 tgccgtcaag cgcgccgccg ttgatttggt tgcgcgtgtg gtaggggagg aagcggcttc
      781 cttcaaacct gcctttgatt gctgttgtgc gcgcggtgat ggggaatcgg gagaggtcgg
      841 cggtatgtgt gccgccggta ttgtcgattg tgccgctgtt ttttcccgtc tgcctgatgc
      901 ggacaagggc ttttccgctc cgcaaaccgg caggcagggg gacggagata aaatcgtcgg
      961 gaataccgta aatcgggaag cgggcttgtg ccgtccgcct gtcgtcgccc ttcagcaccg
     1021 gttcgtaata gccggtaacc gtaccggcaa ggcttccgtt gcctgcaacc tgccacggcg
     1081 tgaaatagcg ttcaaaaaac tgttttgcct gaaaggaatg gacgggggtt tgaaaggctt
     1141 gggcgcacac atcctgccag ccttggcggt ttttcaaatt ggcgcagccg aggcggaagg
     1201 attgcaggct tttggcgaaa tcctgcgccg cccagtgggg cagggacagg tgcggtacaa
     1261 cggtatagac ggccccgccg ccgccgaccg tcgttccggc ggggtcgggg atgccgaccg
     1321 gccggtccgg gccgttgatg acggatgtgt cgggttgcgg aaaggtttgg atgctcttgc
     1381 tttggcaggc ggcgaggatg gcggcggcga tgccgtacag ggcggcgcgg aataggtatt
     1441 ttttcataat ggacaatgtt gccggcagta ataagaaaga tggtttcggg cggcgttgcg
     1501 gcagccgtgg agaggggatt ttaacacagg gcgcagctgc agcctgcgga actttccgcc
     1561 gcgcggtact gcagataaaa ataacttgca tttgtattta caagcaatga aaatattccg
     1621 ataatatatt attcatcatc cttgttcgtt cgcgtttatg ctggtcgctt ttttaattat
     1681 gttgcgcgag ggtattgaag ccgcgctcat tgtcggcatc gttgccggtt ttctgaaaca
     1741 gtccggacat tccaaactga tgcctaaggt ctggttcggg gtcgtccttg cttctttgat
     1801 gtgtttgggg ctggggtacg gcatccattc ggcaacgggc gagattcccc agaagcagca
     1861 ggagttcgtc gtcggcatta tcggtttggt tgccgttgcc atgctgactt atatgatttt
     1921 atggatgaaa aaggcggcgc gttcgatgaa gcggcagctt caggattctg tgcaggcggc
     1981 tttgaaccgt ggcagcggtc aaggatgggc cttggtcggt atggcgtttc ttgccgtggc
     2041 gcgcgaaggt ctggagagtg tttttttcct gcttgccgta ttcaaacaga gcccgacgtg
     2101 gcagatgccg gccggcgcgg tagcgggggt tttagctgcc gccgtgattg gcgcgttgat
     2161 ttatcagggc gggatgcgcc tgaatctggc gaagtttttc cgttggacgg gggcgtttct
     2221 gattgtcgtt gccgccggcc tgcttgccgg ctcgctgcgc gcgctgcatg aggcaggtat
     2281 ttggaacgcg cttcaggaca ttgtgttcga ctcatcaaaa tatttgcacg aagacagtcc
     2341 gttgggcgtg ctgctcggcg gatttttcgg ctataccgac catccgacgc agggcgagac
     2401 cttggtttgg ctgctgtacc ttattcccgt cataacttgg tttttgtgcg gcagcaggcc
     2461 gtctgaaact ttaacccgta aagaggagct gaaatgagaa aattcaattt gaccgcattg
     2521 tccgtgatgc ttgccttagg tttgaccgcg tgccagccgc cggaggcgga gaaagctgcg
     2581 ccggcagcgt ccggtgaggc gcaaaccgcc aacgagggcg gttcggtcag tatcgccgtc
     2641 aacgacaatg cctgcgaacc gatggaactg accgtgccga gcggacaggt tgtgttcaat
     2701 attaaaaaca acagcggccg caagctcgaa tgggaaatcc tgaaaggcgt gatggtggtg
     2761 gacgagcgcg aaaacatcgc ccccggactt tccgataaaa tgaccgtcac cctgttgccg
     2821 ggcgaatacg aaatgacttg cggtcttttg accaatccgc gcggcaagct ggtggtaacc
     2881 gacagcggct ttaaagacac cgccaacgaa gcggatttgg aaaaactgtc ccaaccgctc
     2941 gccgactata aagcctacgt tcaaggcgag gttaaagagc tggtggcgaa aaccaaaact
     3001 tttaccgaag ccgtcaaagc aggcgacatt gaaaaggcga aatccctgtt tgccgacacc
     3061 cgcgtccatt acgaacgcat cgaaccgatt gccgagcttt tcagcgaact cgaccccgtc
     3121 atcgatgcgc gtgaagacga cttcaaagac ggcgcgaaag atgccggatt taccggcttt
     3181 caccgtatcg aatacgccct ttgggtggaa aaagacgtgt ccggcgtgaa ggaaattgca
     3241 gcgaaactga tgaccgatgt cgaagccctg caaaaagaaa tcgacgcatt ggcgtttcct
     3301 ccgggcaagg tggtcggcgg cgcgtccgaa ctgattgaag aagtggcggg cagtaaaatc
     3361 agcggcgaag aagaccggta cagccacacc gatttgagcg acttccaagc caatgtggac
     3421 ggatctaaaa aaatcgtcga tttgttccgt ccgctgatcg aggccaaaaa caaagccttg
     3481 ttggaaaaaa ccgataccaa cttcaaacag gtcaacgaaa ttctggcgaa ataccggact
     3541 aaagacggtt ttgaaaccta cgacaagctg ggcgaagccg accgcaaagc gttacaggcc
     3601 tctattaacg cgcttgccga agaccttgcc caacttcgcg gcatactcgg cttgaaataa
     3661 gccgcaagcg ttcagacggt atttggcggc agataccgtc tgaagtttta tagtggatta
     3721 acaaaaacca gtacggcatt gcctcgcctt gccttgccgt actatttata ctgtctgcgg
     3781 cttcgtcgcc ttgtcctgat ttttgttaat ccactatatc cgccatatat tgcagggcgg
     3841 gatttcaacc tgccgctatc ggttaatgga aaaacggcgt gcagggatac ccatcctgct
     3901 gcacggatat tgaaggaaac accatgagca aaaaacaacc cgcacaaccg accaggcgca
     3961 ctctttttaa aaccgcgatc gcagccggag cagtcggcgc aatcggaggt tatctcggcg
     4021 gcaaaaaaca gggcgaaacc gccgaacgca ccgccgaaag ccaacactcg ccccaagcct
     4081 atccctgcta cggcgaacat caggcaggca tcgttacgcc gcagcaggcg ttttcgatta
     4141 tgtgcgcctt cgacgtaacc gcgcaaagtg ccaagcagct ggaaaacctg ttccgcacgc
     4201 tgaccgcccg catcgagttt ctcacccaag gcggcgaata ccaagacggc gacgacaaac
     4261 ttccgccagc cggcagcggc attttgggca aagccttcaa ccccgacggg ttgaccgtta
     4321 ccgtgggggt gggcagcagc ctgtttgacg gccggttcgg actcaaagac aaaaaaccga
     4381 ttcatttgca ggaaatgcgc gacttctcca acgataagct gcaaaaaagc tggtgcgacg
     4441 gcgatttgag cctgcaaatc tgtgccttca cccccgaaac ctgccaagcc gccctgcgcg
     4501 acatcatcaa acacaccgtc caaaccgccg ttatccgttg gagtatcgac gggtggcagc
     4561 ccaaatccga acccggcgcg atggcggcgc gcaacctgtt gggcttcagg gacggcacgg
     4621 gcaaccccaa agtttccgat cccaaaactg ccgacgaggt tttgtggacg ggggtggccg
     4681 ccaacagcct cgacgaaccg gagtgggcga aaaacggcag ctatcaggca gtccgcctta
     4741 tccgccactt tgtcgagttt tgggacagga cgccgcttca agagcaaacc gacattttcg
     4801 ggcggcgcaa atacagcggt gcgccgatgg acggcaaaaa agaagccgac caaccggatt
     4861 ttgccaaaga ccccgagggt gatatcacgc ccaaagacag ccatatacgc ctggcgaatc
     4921 cgcgcgatcc cgaattcctc aaaaaacacc gcctcttccg ccgcgcctac agctattcgc
     4981 gcggactcgc ctcaagcgga cagcttgatg tcgggctggt gttcgtctgc tatcaggcaa
     5041 accttgccga cggattcatc ttcgtgcaaa acctcctcaa cggcgaaccg ctggaagaat
     5101 acatcagccc cttcggcggc ggctatttct tcgtcttgcc cggcgtggaa aaaggcggct
     5161 ttttggggca agggctgctg ggcgtataaa tccgccatat aaaaaacgcc gtccgaacct
     5221 tgccaaacgg gttcggacgg cgtttcttgt ttttgggcgg tcaggctttt ttgacgaatt
     5281 cggattttaa attcatcgcg ctgccgtcga ttttgcagcc gatgttgtga tcgccttctt
     5341 gcaggcgtat gcctttgact tttgtgcctt gtttgatcac catcgagctg ccttttacct
     5401 tgaggtcttt gatgaggatg acggtatcgc cgttttgcag cactgcgccg ttggcatcgc
     5461 gcacttgagc cgcaaggtcg gcggcggatt cggtttcatt ccattcatgg gcgcattcgg
     5521 ggcagatgta ttgtccgccg tcttcatagg tgtattcgga ggcgcattgc gggcatgggg
     5581 gtaatgacat ggtttgccgt ccttatcgga tgtttgtttt ggggtgccgt ctgaaacctg
     5641 aaaccggctt cagacggcat agctttattg tttgtctttt tcaggacgca cccagccttc
     5701 gatgacggtt tggcgggcgc gggcgagggc gagtttgttg tcttcgacat tgcgggtaat
     5761 cgtgctgccc gcgcctgtgg ttactttgtt gccgagggta acgggggcga ctaggacgca
     5821 gtttgaaccg atgcgcactt cgtcgccgat gacggttttg tgtttgtgca cgccgtcgta
     5881 gttggcaata atcgtaccgg cgccgaagtt ggttttgcag ccgacttcgg cgtcgccgat
     5941 gtaggtgagg tggttggctt tggtgccttt gccgatggcg gcgtttttga tttcgacgaa
     6001 gttgccgacg tgtacgtcgt ctgcaaggcg ggcttgcgga cgcaggcggg cgtacgggcc
     6061 gattcggttg ttttcgccga cttcgcagct ttcgaggtgg gagaaggggg cgattttgct
     6121 gtttgcgccg attttggcgt ttttgatgac gcagtttgcg ccgatttcga cgttgtcgcc
     6181 gagctcgatg tcgccttcaa agatacagtt cacatcaatc acgacgtctt gcccgtgttt
     6241 cagacggcct cgtaaatcga aacgtgccgg atcgcgcagg gttacgcctg ctttgagcaa
     6301 ttcttgcgcc tgttcggttt ggaagatgcg ttcgagttcg gtgagctgga gtttgttgtt
     6361 cacgccggcg gcgaggtggg aggcgcgcac ttggacggga tgaactttaa taccgtcggc
     6421 aacggctttg gcgatgaggt cggtcaggta gtattcgcct tgtgcattgt tgctggaaag
     6481 gctgttcagc cagttttcga gtttggcgtt gggcaggacg aggatgccgg tattgatttc
     6541 tttcacggct ttttggacgg cgtcggcgtc tttttcttcg acgatggcgg ttacgctgcc
     6601 gttgctgtcg cggatgatac gccccaagcc tgtcgggtcg ttgggaacgt cggtcaacag
     6661 cccgacttcg ttgcctgcgg cttcgagcag ggtttcgagg gtttcaacgt caattaaagg
     6721 aacgtcgccg tacaacacca gcgtgcggcc ttcggcggaa aggtggggca gggcggtttt
     6781 gacggcgtgg ccggtaccga gctgttcggt ttgttcaacc caaacgacat cgcgtttgac
     6841 ggtgtccaag acttgctctt tgccgtggcc gatgacgacg cagatgtttt gcggattcag
     6901 tgcggctgcg gtgtcgataa cgcgcccgac catgggcttg ccgccgatgc ggtgcagcac
     6961 ttttggcatt ttggaataca tgcgcgtgcc tttgccggcg gcgaggatga cgatgtttaa
     7021 agtgttttgc ggcatgacgg tttcctgtgc aatgccgtct gaagcggctt cagacggcat
     7081 agggtaggtt tatcggtttt gaaactttgg tttttgccag tgttggcgat gctcttcgtc
     7141 ggcgttgttg ccggtttggt tgggtaacac ggcatggcgt tcgggacggt attggttgta
     7201 gttcatattt ttcgagtagc tgccgtcttg gtaataaacg ggcgtgccgg cgggatattt
     7261 ttgacggacg gcggtcttgc cgttgccgtc ttgataagtt tcccacgcgc agcccgacaa
     7321 aagggcggcg gcagcggtca ggaagaggaa ggttttacgc atggcttttc tttcgtattt
     7381 tcggggggta gggggtattg taatgatttt ggcggtgttc tgacaaagtt tctgcatacc
     7441 gagccagttg cgccatatcg cttacggagg catcgataaa gggcagcgcg tgggattttg
     7501 caccgaaccg gacggttttc atacccagcg cctttgcctg atgcaggttg tccgcgctgt
     7561 cgtccaccat aatgcagcat tcgggcggta cgtccaacag gcggcagaca ttgagatacg
     7621 cttgcggatt gggtttgtac agcagcccga aatcatccgt gccgaaaagc gcgtcgaaac
     7681 ggttttccaa accgagtgcg ttgacaacgg cacggacgta aaacgacggg ccgttggaaa
     7741 aaaccgcctt gcgccctttt aggcggctca gggtgttttg tgtttcaggc atgccgtgca
     7801 gcctggtcag gattgcatcg atcggatggc tttcgcgcaa aaattcggcg atgtcgattt
     7861 cgggatggtg gatttgcagt ccggcgagcg ttgcgccgta gcggtgccaa tagtcttgac
     7921 gcaggtcgga cgcggcagat tcggagagtt tgaggcggcg tgccatatag cgtgtcatag
     7981 cgcggttgat gagtgtgaag atgcctgcgt cggcatcgtg cagcgtgttg tcgaggtcga
     8041 acagccacac ggtcgggttt tcttgcatgt tgaaccgtga aaatttgtta gaatgttatt
     8101 ttacagcgaa tagaggagga ctcggaatga aacggaaaat ttggctgctg ccgctgctgg
     8161 cggtttcggc atacctgcag gcgcagacgg aagtcaggct ggcggtgcat aagtcgttca
     8221 gcctgcccaa agggttgatt gcgcgcttcg agcgggcaaa cgatgcgaag gtgtcgatta
     8281 ttcaggcggg cggcgcgaac gaaatgctca acaaactgat tttgagccgc gccaacccga
     8341 ttgccgacgc ggtgtatggt ttggacaacg ccaatatcgg caaggcgcgg gaaatgggca
     8401 ttttggcggc ggcgcaaccc gaatccgccc ccgtcgcggt cgggctgcct tcggctttgg
     8461 cggtcgatta cggctatgtg tccatcaatt acgacaaaaa atggtttgaa ggcaaaaagc
     8521 tgcccctgcc gcaaaccctg caggatttga cccgccccga atataaaaac ctattggtcg
     8581 tgccgtcccc cgccacgtcg tccccggggc tgggcttcct gatggcgaac atcagcggtc
     8641 tgggcgaaga aagcgcgttc aaatggtggg cacagatgcg gcagaacggc gtgaaggtcg
     8701 ccaaaggctg gagcgaggcg tattacaccg acttttcgca caacggcggc gcgtatccgc
     8761 tggtggtcgg ttatgccgcc agcccggcgg cggaagtgta tttttccaaa ggcaaataca
     8821 gcgagccgcc gacgggcaac ctgtttttaa aaggcggcgt attccgccag gtcgaaggcg
     8881 cggcggtctt gaagggcgcg aaacagccgg aattggcggc aaaactggtg caatggctgc
     8941 aaagtcggga agtgcagcag gcggttccgt ccgaaatgtg ggtttacccc gccgtcaaaa
     9001 acacgcgcct gcccgacgtg ttccgcttcg cccaagcccc gacgcacacc accgcccccg
     9061 cgcagcgcga tattgatgcg aaccagcgcg gatgggtttc ccgttggatt agaacggttt
     9121 tgaaataaaa caaacatacc tccccgcagg gcttcatacg gcatttttac acctgtgccg
     9181 attacgccgc acggggcgga tgttcgatca agaggaaaac aatggacttc aaacaatttg
     9241 attttttaca cctgatcagt gtttccggtt gggagcatct ggctgaaaag gcgtgggcgt
     9301 tcgggctgaa ccttgccgcc gcgctgctta tttttttggt cggaaaatgg gcggcgaaac
     9361 gcattgtcgc tgtgatgagg gcggcgatga cgcgcgcgca ggtcgatgcc acgctgatta
     9421 gttttttgtg taatgttgcc aatatcggct tattgatttt ggtgattatt gccgcattgg
     9481 gcagattggg cgtttccaca acatccgtaa ccgccttaat cggcggcgcg ggtttggcgg
     9541 tggcgttgtc cctgaaagac cagctgtcca attttgccgc cggcgcactg attatcctgt
     9601 tccgcccgtt caaagtcggc gattttatcc gcgtcggcgg ttttgaagga tatgtccgag
     9661 agattaaaat ggtgcagact tctttgcgga cgaccgacaa cgaagaagtc gtgctgccca
     9721 acagcgtggt gatgggcaac agcatcgtca accgttccac actgccgctg tgccgcgccc
     9781 aagtgatagt cggcgtcgat tacaactgcg atttgaaagt ggcgaaagag gcggtgttga
     9841 aagccgccgt cgaacacccc ttgagcgttc aaaacgaaga gcggcaggcc gccgcctaca
     9901 tcaccgcctt gggcgacaat gccatcgaaa tcacattatg ggcttgggca aacgaagcag
     9961 accgctggac gctgcaatgc gacttgaacg aacaagtggt cgaaaacctc cgcaaagtca
    10021 atatcaacat cccgttcccg caacgcgaca tacacatcat caattcttaa acgccgtctg
    10081 aaagaggagt gggaaatgga cgcgctgcac accatcgccc gaaacctgac gaaaaaacgt
    10141 caaaccgtaa gctgtgccga atcctgtacg ggcggaatgc ttgccgccgc attcacaagc
    10201 gttgcaggca gttcgcaatg gttcgaccag agttttgtaa catacagcaa caaagccaaa
    10261 gaagaccgct tgggcgtgtt gcccgaaacc ctgctcgaac acggcgcggt cagccgccaa
    10321 accgtctatg agatggcgcg cggcgcgaaa gccgtggcgc aggcggatta cgccgtcggt
    10381 atttccggca tcgccggtcc gggcggcggc agcgaaagca aacccgtcgg cacggtttgg
    10441 ttcgggtttg cctttccggg cggaagttgc gaagcaatgc gccgttttga cggcaaccgc
    10501 gaatccgtcc gcgcgcaggc ggtcgccttc gcgttggaac ggttggcggg gctgattgaa
    10561 aacggcggcg atgctgtcta aacaaaatct ccgtctgaac aaaatcccca tcggataaaa
    10621 aatgccgtct gaaacgtttc gggtttcaga cggcattttg tcggggtagg cggcggtgcg
    10681 gcttatttca ctttaccttt caacgcgcca tagcctgccg cgtccatttg ttccagcggg
    10741 atgaatttca agctcgcgcc gttgatgcag tagcgcagtc cgcctttgtc gcgcgggccg
    10801 tcggggaaga cgtgtcccaa atgcgagtcg gcggcgtggc tgcgcacttc ggtgcggcgc
    10861 atgttgtagc tgaaatcatc gtgttcggta acggattttg catcaatcgg gcgcgtgaag
    10921 ctcggccagc cgcagccgga atcatatttg tcggcggagc tgaacaaagg ttcgccgctg
    10981 acaacgtcca cataaatgcc gggtttgaac aaatggtcgt attcgtggct gaaggcatat
    11041 tcggtcgcgc tgttttgggt aacttggtat tgctcttcgg tcagggtgcg tttgagttcg
    11101 gcgtcactcg gttttttata cgttgccgcg tcgaagcctt tgccttgcgg ggcggtcttg
    11161 gttttgcccg gcagcggttc gtcagctttg cggatgtcga tgtggcagta gccgttgggg
    11221 tttttaatca agtagtcctg atggtattcc tcggcatcgt agaagttttt cagcggctcg
    11281 ttttcaacaa cgaggggcag ttggtatttt tgctgctcgc gtttgagggc ggcggcgatg
    11341 acggcttttt cggcggggtc ggtgtagtac acgccgctgc ggtattgcgt accggtgtcg
    11401 ttgccctgtt tgttgaggct ggtcggatca acgacgcgga agaaatattg caggatgtcg
    11461 tctaggctga gtttgtcggc atcgtaggtc actttgacgg tttcggcgtg gcccgtatgg
    11521 cggtaggaca cgtcttcata gctcggattt ttcgtgttgc cgttggcgta gccggatacc
    11581 gcgtcaacca cgccgtcgat gcgttggaaa taggcttcca agccccagaa gcagccgccg
    11641 gcgaggtaaa tggtgcgcgt gttcatgatt tttgaatcct ttttctgagt gtcgggtttg
    11701 tagaacgaat gtttcaagct gcccaaatcg gcattcgggt cgcggattaa cgccaacgcc
    11761 tgcgcttcgt tgatgctgcc tttgacgatg cgctgcacgt cgccgtcttt accgattaac
    11821 gcccacgagg ggtaaacgct gatattcagg ctttgggcga tcgtgccgcc gttgtcggtt
    11881 acgacgggca gcttgggata attcaaaccg gcataccatt tttggaagtc gccgtctttt
    11941 ttctcgtgca aaaagcccgg ggaggcgacg gtaatcaggt tggcggagct gaattttgca
    12001 tcttgcgccc atttttcggt ctgtcccaat tcggacagac acaaaggaca ccagctcgcc
    12061 caaaatttaa tcagcgtcgg tttgtctttt ttcaagtaaa cactggcggg gcggttgtcc
    12121 gcagttttca aagtggataa agtgtgcggc acggtcgcgg ctccggcatc gacgattttg
    12181 ggcgaacaag cacccagcgc aagcaggcag ccgaacttgg cgcaaaggga aaagaaagta
    12241 cggtgtttca ttttgatgtt tcctgtgtgg acggtttgca tgattagacg tttgagatgc
    12301 cgaaacctta cagcccggat tttcagacaa ccttaccgcg taaaatacgc tacaatacgc
    12361 cctgtttcaa gtttctaaaa ttaaaaggaa aattcaatgt tcagcttctt ccgtcgcaag
    12421 aaaaaacagg aaacgccggc tctcgaggag gctcaaattc aggaaaccgc agcaaaagca
    12481 gaatctgaac ttgctcaaat agttgaaaat attaaagaag atgctgaatc tttagcagaa
    12541 agcgtcaaag ggcaggtcga atctgccgtt gaaaccgtca gcggtgcggt tgaacaggta
    12601 aaggaaaccg ttgccgagat gctgtctgaa gcagaggaag cggcggaaaa agcagcggaa
    12661 caagtcgaag cggcaaaaga agccgttgcc gaaaccgtcg gcgaggctgt cgggcaagtt
    12721 caagaagccg ttgcgacaac tgaagaacac aagctcggtt gggcggcgcg tttgaaacaa
    12781 ggcctgacca aatcgcgcga caaaatggcg aaatcgctgg cgggcgtgtt cggcggcgga
    12841 caaatcgacg aagatttata cgaagagctg gaaaccgtgc tgattaccag cgatatgggc
    12901 atggaagcca ccgaatacct gatgaaagac gtgcgcgacc gcgtcagcct caaagggctg
    12961 aaagacggca acgaattgcg cggcgcgttg aaagaagcct tgtacgacct gattaagcct
    13021 ctggagaaac ctttggtttt gcccgaaacc aaagagccgt ttgtcatcat gcttgccggc
    13081 atcaacggcg cgggcaaaac cacgtctatc ggtaaactcg ccaaatattt ccaagcgcag
    13141 ggcaaatccg tattgctggc ggcaggcgat actttccgtg ccgccgcgcg tgagcagctt
    13201 caagcttggg gcgagcgcaa caacgtaacc gtgatttcgc aaaccacggg cgattccgcc
    13261 gccgtgtgct tcgatgccgt ccaagccgcc aaagcgcgcg gcatcgacat tgtgctggcc
    13321 gacaccgccg gccgcctgcc cacgcagctt catttgatgg aagaaatcaa aaaagtgaaa
    13381 cgcgtgctgc aaaaagccat gcccgacgcg ccgcacgaaa tcatcgtcgt gcttgatgcc
    13441 aatatcgggc aaaacgccgt caaccaagtc aaagcctttg acgacgcatt ggggctgacc
    13501 ggtttaatcg ttaccaaact cgacggcacg gcaaaaggcg gcatcctcgc cgcgcttgcc
    13561 tccgaccgcc ccgttcccgt ccgctatatc ggcgtgggcg aaggcataga cgacctgcgc
    13621 ccgtttgacg cgcgcgcgtt tgtggacgca ctgctggatt gagccgaaat gccgtccgaa
    13681 aacagcagac cgatgccgtc attcccgcgc aggcgggaat ccagaccttg ggataacggc
    13741 aatattcaaa ggttatctga aagtccgaga ttctggattc ccactttcgt gggaatgacg
    13801 ggatgtaggt tcgtgggaat gacgtggtgc aggtttccgt atggatggat tcgtcattcc
    13861 cgcgcaggcg ggaatctaga acgtaaaatc taaagaaacc gtgttgtaac ggcagaccga
//
LOCUS       DAWGRZ010000040        13593 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000040 DAWGRZ010000000
VERSION     DAWGRZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13593
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.040"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(225..548)
                     /locus_tag="WHT44_000813"
     CDS             complement(225..548)
                     /locus_tag="WHT44_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ2533025.1"
                     /translation="MDIVQLGPAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(628..1284)
                     /locus_tag="WHT44_000814"
     CDS             complement(628..1284)
                     /locus_tag="WHT44_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ2533026.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDVEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDADPFGGFDF
                     "
     gene            complement(1387..1914)
                     /locus_tag="WHT44_000815"
     CDS             complement(1387..1914)
                     /locus_tag="WHT44_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ2533027.1"
                     /translation="MYATIGDVMHALHFSASDKAALYREVLPQIESVVADEADWVANL
                     ANTAAVLKEAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGET
                     VVVKDVNAHPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGEL
                     AKILEKRFEASRQAV"
     gene            2074..2847
                     /locus_tag="WHT44_000816"
     CDS             2074..2847
                     /locus_tag="WHT44_000816"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ2533028.1"
                     /translation="MYRQIGMWDQKWVIGNWKMNGRLQNNNALMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPNNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2854..3204
                     /gene="secG"
                     /locus_tag="WHT44_000817"
     CDS             2854..3204
                     /gene="secG"
                     /locus_tag="WHT44_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ2533029.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3218..3303
                     /locus_tag="WHT44_000818"
     tRNA            3218..3303
                     /locus_tag="WHT44_000818"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3252..3254,aa:Leu,seq:gag)
     gene            4113..4481
                     /locus_tag="WHT44_000819"
     CDS             4113..4481
                     /locus_tag="WHT44_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ2533030.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            4478..4789
                     /locus_tag="WHT44_000820"
     CDS             4478..4789
                     /locus_tag="WHT44_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ2533031.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(5172..6101)
                     /locus_tag="WHT44_000821"
     CDS             complement(5172..6101)
                     /locus_tag="WHT44_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ2533032.1"
                     /translation="MLRCRPKSVLDSDGIFLKFNFLRSKPKYEISFLPSFKRILCLSA
                     VISVLGACAVVADVYGHDSATMNAAAAKDYMKTVELNKSAGNVDTTSRTARRVQAVFR
                     RMLPYADAANNTSHKFDWKMTVFKNDELNAWAMPGGKMAFYTGIVDKLKLTDDEIAAI
                     MGHEMTHALHEHGKNKVGQQILTNTAAQIGTQIILDKKPDTNPELVGLGMDILGTYGL
                     TLPYSRSLEEEADEGGMMLMAQAGYHPAAAVRVWEKMNQENDQNGFIYAITSTHPTNN
                     ARIENLKRLLPTVMPVYEQSVRNKGRVNKKRRR"
     gene            complement(6124..6255)
                     /locus_tag="WHT44_000822"
     CDS             complement(6124..6255)
                     /locus_tag="WHT44_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HEZ2533033.1"
                     /translation="MRDFDKPAEEAAEYAAEGAVKFIEGKPAKKIIAVPGRLENIVV"
     gene            complement(6375..7178)
                     /locus_tag="WHT44_000823"
     CDS             complement(6375..7178)
                     /locus_tag="WHT44_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003743893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ2533034.1"
                     /translation="MNLFFDTELGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYHTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPNHIMCSINLAPLPESGKIFLIDNS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYEFENKLSIQ
                     FPQNNHIRDKIRQQLQILRDQNTIEFIGRGLYKKSINCAQLPRYFDFKS"
     gene            complement(7312..9942)
                     /locus_tag="WHT44_000824"
     CDS             complement(7312..9942)
                     /locus_tag="WHT44_000824"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ2533035.1"
                     /translation="MQEQYRPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     AAAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFATKYNLPKKQVIAVGDNAFDENQWQEWYGDKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     QCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVDPAAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPISAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGEAVKAFAGSQDGLSKELKDLRHKLHATTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGGEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            10212..10727
                     /locus_tag="WHT44_000825"
     CDS             10212..10727
                     /locus_tag="WHT44_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ2533036.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDESMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            10734..11177
                     /locus_tag="WHT44_000826"
     CDS             10734..11177
                     /locus_tag="WHT44_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ2533037.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDTVGDFIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            11346..13403
                     /gene="ppk1"
                     /locus_tag="WHT44_000827"
     CDS             11346..13403
                     /gene="ppk1"
                     /locus_tag="WHT44_000827"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ2533038.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDQNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKREHKRCPQRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELAQEGIHFYRRRNWTDTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPAVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGLITADEQITADVNILFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIVGRQLEHARVYYFHNNGTDDTFISSADWMGRNFFRRIET
                     ATPITAPELKKRVIHEGLTMALDDNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            13428..>13593
                     /locus_tag="WHT44_000828"
     CDS             13428..>13593
                     /locus_tag="WHT44_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533039.1"
                     /translation="MPSETFPFQTAWFYSNLNRADRSQKHTFAIPAQALPLYAPNPKP
                     PHSRRHYSGLN"
BASE COUNT         3336 a         3848 c         3329 g         3080 t
ORIGIN      
        1 gcctgcacat tgaaaatgcc gcccaagcaa actttcagtt tgcccgcctc gtcctagccc
       61 tctcccacgg gagaggggat tggggtgcag gctgccttta aggttcaggc aaattttaac
      121 ttcgttgata ccgcgcttta gcttcgcaga agctgcactt tcagacgacc ttttggaaca
      181 ccacaggtac acgcatttaa ggaatgccgt ctgaaatgcc tgcctcaata acgcatcatg
      241 ttgccgtcaa tctcggccgc ccatgcatcg atgccgccct gaaggttgta caggttttca
      301 aaacccgcgt ccgccaaata catcgccgta tgcaggctgc ggataccgtg gtggcaatac
      361 accacaagcg gcacatcatc cggcagctcg ttctgccgca gcggaatcag attcatcggg
      421 atatgcagcg catttggcag cgaacaaacc gccgtttctt catccgtacg cacgtccaac
      481 aaacaaaaca tccgcccttc gtccatccac gctttcaatt ccgcgggccc aagttgcaca
      541 atatccatcg caccccccaa aaaaaaccaa gcaaaatgcc gtctgaagcc ccaaacccgc
      601 tttcagacgg catgacctgt caacatctta aaaatcgaaa ccgccaaacg gatcggcatc
      661 cttatcatcc aaatgcgcca ccaaggtatc gaacagcacc ttctcttcaa acacatcgcc
      721 cctgcgcgta atcaaaagcg cgcgttgaac aggcttgcga cctacgataa ccaccatgcg
      781 tccgccatct ttcaactgtt ctttcaacac ttcaggcaca aggtttaccg caccgccgac
      841 ataaaccgca tcaaacggcg cacctgcgga aagttcggtc aacccgttgt tttgcacata
      901 atcgatattg tccaaaccca agccgtccaa caccgctttg gcgcggtttt gctgttcgac
      961 atcgatgtcg tccgacacca cacgaccagc caattttgcc aacagcgcgg tcgcatagcc
     1021 cgaacccgtg ccgatttcca aaaccgtatc gtttttcgtc agcttcaagc cctgcgccag
     1081 ccgcgccacg actttcggct cgagcatctt atgaccgttg gcaagcggca gcgccatatc
     1141 cgcatacgcc aaaccctgca agtcctcatc gacaaaaagc tcgcgcggaa tctccgccaa
     1201 agcgtccaac acatcaaaat ccaatacatc ccacggacgg atttgctgtt cgaccatatt
     1261 gaaccgcgct ttttcaaaat ccatttagtg ctccgtcaaa taattgttcc aacaatggca
     1321 ggcattataa aaccacactc ccgccgcgcc aacttcggca cgcgccgaca gcccgttttg
     1381 tcagtctcaa accgcctgac gcgaagcctc aaaccgcttc tccaaaatct tcgccagttc
     1441 gcccaaatac aaagcatccg tctcatcaaa ctgcgccaaa tgttcgctgt ccgcgtccaa
     1501 cacgccgata cagcggccgt ctgaaaacag cggcacgaca atctccgaac gtgacaaaga
     1561 cgaacaggca atatggtcgg gatgcgcgtt gacatcctta acaaccaccg tttcaccctt
     1621 cgcccaagcc tgaccgcaca ccccgcgacc gaacggaatc cgcgtacacg ccaaaggccc
     1681 ctgaaacggt gccaaaacca attcgtccga acgcgtatcg accaaataaa aacccaccca
     1741 aaaccaaccg aacgcctcct tcaaaaccgc cgccgtgttc gccaaattcg ccacccaatc
     1801 cgcctcgtca gccaccacag actcaatctg cggcaacacc tcccgataaa gcgcggcctt
     1861 gtccgaagcc gaaaaatgaa gcgcgtgcat cacatctcct atagttgcat acatatcagg
     1921 cggccattat aaaacaacct gcccgaaaca acattccgaa ccgcccccgc agccgcccgc
     1981 cccaagttgc gaacccgccg catatccact aaacttcaca ttgcaacgcg ccacacgcgg
     2041 cagacagaaa aaacacgaca cggagcaaaa aagatgtatc gccaaatcgg aatgtgggat
     2101 caaaaatggg tcatcggcaa ctggaaaatg aacggccggc tccaaaacaa caacgcactg
     2161 atgcaccgct tccgcatcct ccccaccgcc gaacgcgtcc tcatcggact cgccgccccg
     2221 accgtttacc tgctgcaact gcacaacgcc atgcaaatcg tcttaaacaa ccgcatcctc
     2281 acctgcgccc aagacgtgag ccgcttcccc aataacggcg cgtacaccgg cgaagtgtcc
     2341 gccgaaatgc tcgccgacac cggcacagac atcgtcctca tcggacactc cgaacgcagc
     2401 ctttatttcg gcgaaaaaaa cgaaatccaa cgccgcaaaa tggaaaacgt cctcaacgtc
     2461 ggactcatcc cgttattgtg cgtcggcgaa agcctcgaag agcgcgaagc cggcaaagaa
     2521 cacgaagtca tcgcccatca gctttccatc ctccaaggac tggacaccaa aaacatcgcc
     2581 gtcgcctacg aacccgtctg ggcaatcggc accggcaaag tcgccaccgt cgaacagatt
     2641 gccgatatgc acgcattcat ctacaaagaa atcttgtctt tgtgcggaag cgatgttaaa
     2701 atccgcgtcc tttacggcgg aagtgtgaaa gcggacaacg cggccgacat cttcgcagta
     2761 ccttatgtgg acggcgcact cgtcggcggc gcgtcattgt cgtacgactc ctttaccgcc
     2821 atcatcagtg ccgcacaaaa tgcgtagaaa aatatggaag ccttcaaaac cctaatttgg
     2881 attgttaata taatttccgc tttggccgtc atcgtgttag tattgctcca acacggcaaa
     2941 ggcgcggatg ccggcgcgac tttcggatcg ggaagcggca gcgcgcaagg cgtattcggc
     3001 tctgccggca acgctaactt cctcagccgc tcgaccgccg ttgcagcaac atttttcttt
     3061 gcaacctgca tggctatggt gtatattcac acccacacaa caaaacacgg tttggacttc
     3121 agcaacgtac aacaaactca gcaagcaccc aaacccgtaa gcaataccga accttctgcc
     3181 cctgttcctc agcagcagaa ataacagttt ttcaaatgcc gacatggtga aattggtaga
     3241 cacgctatct tgagggggta gtggccgtag gctgtgcgag ttcaaatctc gctgtcggca
     3301 ccaacacaca aaacgcttag aaattctaag cgtttttttc gtttaatcct gtctaatgcc
     3361 gtcaaccaat cccaatcaat acaaggcttt cagactggtt gctatcctat tgtcttcttc
     3421 ctgattgtat aattccgttt aattcagcct aattgaagta acctaaaatt tacggtatct
     3481 tttgcggtat ctgaaaaata cctcgaaaaa ataccgcaaa aataaagctg aacgaccacc
     3541 aaatcaggaa tgccaagcgg gaaatagctt gcggggaata ctgacgcacc gtagggaatg
     3601 agggggaaac cgtccaagat gcagagcggt tttttttggg tttttggaaa aaacctatac
     3661 tatagtggat taaatttaaa ccggtacggc gttgcctcgc cttgccgtac cggtttaaat
     3721 ttaatccact ataaaataag aaaaagataa aaaattggta acaaatgcgg taacaaatgg
     3781 taacgaatcg gtaacaactt ttggggtttt ccggtttttc accgtcttgg cagtgggagc
     3841 gtagcggaat gaaaagccaa aacgcacgga accgcgccta ttttgagcag gaatggcggt
     3901 taaaccgctt ggttatatac tggcatagga agacagcgaa acgcgctaaa aacgcaattt
     3961 gagccattaa aagcgattga ttaaaaaata atcaggttag ccgccggtgt ttcaggcggc
     4021 ataaacggag aaattgcagg gtataaaaag gcgacttgcc gccttgcctg tctctggtat
     4081 aattccaagt atcactaatc aacggctaca caatgcggat attcaaaaac caatggatag
     4141 tgaaatttgc caagaagcac aaaatcaacg attccgagct gctggaagcg gtagagcggg
     4201 cggataacgg gctgatagac gcagatttag gcggcggtgt gattaagcag cgcatagcaa
     4261 ggcaaggaca aggcagaagc ggcggctatc gcagcctgat attgttcaaa caggcagata
     4321 gggcattttt cgtttacgcc tttgccaaga acgacaggga aaacatttcg gataaagaac
     4381 tggacgttta ccgaaaagcc gccgcatatt atctgaaata cacgcgggca gagctggcgg
     4441 ctttgaaaga agacggcatt atcacggaga tagaatcatg aaatacaaaa gcgaggcatt
     4501 agccgccatt catgaaatga tggaaggggc ttacaacatc ggcgcaatcg acaaaaagac
     4561 tatgcgcggc tttgacaagt cctgcctgac cgaaatcaaa ccgttgagcg gcggggacat
     4621 caaggcaatc agggagaagg aggcactatc gcaagccgct ttcgccatct atctcaacgt
     4681 gggaaaaaat cacgtttcgg cttgggagcg gggcgttaaa aagccgagcg gcgcggcgtt
     4741 gaagctgctg accatcgtca aaaacaaggg catcgaagcc attgcgtagc cgacttggca
     4801 aacggcaaaa tcagcaagtt cacaatagac gcgctgctga atatgcctgc caagacaggc
     4861 aagaccgccg aactgaatat cagggcgtag ccgcataaat gcccgaccgc atcaaaccaa
     4921 gccgaaacgg cggcggtgca gacgacatag cccgacagca aggcacggcg cagacgggcg
     4981 cgaaacccga aacatcaccg accgcgaggt acggggattt tttgcgcccg ttgcaggggg
     5041 ggattggatt taagcggcgc gggcttgaag gcaaaacggg tggggcacag aactgtttaa
     5101 atgcagtctg aatctcaaac gatttcagac ggcattttga aacaatggct caaattctcg
     5161 atccccttcc cttaacgccg acgtttttta ttaacgcgcc ccttatttct gacactttgc
     5221 tcataaaccg gcataacggt cggcaacaac cgttttagat tttctatacg ggcattgttt
     5281 gtcggatgag tagaggtaat agcataaata aagccgtttt ggtcgttttc ctgattcatt
     5341 ttttcccaaa ccctgacagc ggccgccgga tgatagcctg cctgcgccat caacatcatt
     5401 cccccctcat cggcttcttc ttccaagctg cggctataag gcaaggtaag accgtacgtc
     5461 cccaaaatat ccatacccaa tccgaccaat tccggattag tatccggttt tttgtctaat
     5521 ataatctgcg tgcctatctg cgccgccgta ttggtcaaga tttgctgccc gaccttattt
     5581 ttaccgtgtt catgcagggc gtgcgtcatt tcatgcccca taatggcggc aatttcgtca
     5641 tcggtcagct tgagtttgtc gactatcccc gtataaaacg ccatttttcc accgggcatt
     5701 gcccacgcgt tcagctcatc gtttttgaaa accgtcattt tccagtcaaa cttatggctg
     5761 gtattatttg ccgcatcggc ataaggcagc atacgtcgaa atactgcctg caccctgcgg
     5821 gctgttctgg atgtggtatc gacattgccg gcagacttgt ttaactcaac cgttttcata
     5881 taatctttgg cagccgcagc gttcattgtg gcggaatcat gaccgtaaac atcagcaacg
     5941 accgcacaag cccccaatac cgagattact gccgacaggc agagtatccg tttaaaggaa
     6001 ggaaggaagg aaatttcata tttaggttta ctccttaaaa aattaaattt caaaaaaatg
     6061 ccgtctgaat ccaaaacgga tttcggacgg catcttaaca ttgtttaatg tttttaaaaa
     6121 gatttacacc acgatgttct ccagtctgcc cggtacggcg atgattttct tggcaggctt
     6181 gccttctatg aatttcaccg cgccttcagc ggcgtattcg gcagcttctt cagccggttt
     6241 gtcgaaatca cgcataaatt gccaataatt ctccaacttt tttacggctg ctgctgcctt
     6301 ttgcggcaat attgcgctga acttcaactg ttttcaaaat ggcagaagaa taaatatccc
     6361 ttgtgaattc agtatcatga tttgaaatca aaataccttg ggagttgggc gcaatttatt
     6421 gattttttgt aaagtccgcg accaatgaat tcgatcgtat tttggtcgcg cagaatttgc
     6481 aactgttggc ggattttgtc tctgatatgg ttgttttggg gaaattggat ggatagtttg
     6541 ttttcaaatt catacatttg cgacaatgtg aattcttcgg ggagttggtc gatacatttc
     6601 ataacagcca gaagccagcc tttgcgctcc gcattttggt tgcgtaaaaa caaattggat
     6661 tgccattttt tcagaacggt ttcgggttcg ataatgcggg aattgtctat taagaatatt
     6721 ttgccgcttt caggcaaagg ggcgagattg atagaacaca taatgtggtt cggccggttt
     6781 ttaatgcctt tatttctggg aataatcata tccggcgtga tgaaatgttt gggtacaagc
     6841 accaattgcc gtatggagta atccgctttt ttatatgcaa gaaagaaaaa gttggggttg
     6901 gtatctgacc ggatgcgctc caacatggtg tgatatgcac cgtcaggcac gctgttgcct
     6961 atggtttttt gatttttact ctttaattca tattgctcgt ggcaatttgg gcaaaagagg
     7021 tctgcaacag gtttgttatt ggcaaatctc tgcatcggct tgcttccgca acaggggcag
     7081 tagccgtttt tttccaacca agcctcgctc attacacgga ttttatgggt tgctttattt
     7141 tgttgctttc ccaattcggt atcgaaaaat aaattcatgt tttggatttt gagatttcag
     7201 ttattcgggg ttcgtcatgc agacaacaca atccacctta aaaaggccgt ctgaaaccct
     7261 gtttccaagt ttcagacggc ctttatccgt gtggctaaac cttaaaagcg gttagacgac
     7321 gatgttcacc agtctgcccg gtacgacgat gattttcttc gccggtttgc cttccatgaa
     7381 tttcaccgcg ccttcggtgg cgagtgcggc ggcttcgagg tcggctttgg atgcgtcggc
     7441 ggcaacagtg attttgccgc gcagtttgcc gttgacttga accatcactt cgatttcgga
     7501 tttgaccaag gcggcttcgt cgactgtcgg ccagcctgct tcccacagtt tcgcgccgtt
     7561 caattcgctc cacagggttt cgcagatgtg cggcacgatg ggccacaaca ggcgtacggc
     7621 ggtttccaat acttcttggg cgacggcgcg tccttgttcg ccgccggtgt cggttttgtc
     7681 gtattggttg agcaattcca tcacggcggc gatggcggtg ttgaactgct ggcggcggcc
     7741 gtagtcgtcg ctgactttgg cagtggtcgc gtgcagtttg tggcgcaggt ctttgagttc
     7801 tttagacaaa ccgtcttggc tgcctgcgaa cgctttgacc gcttcgcctt gcttcaagta
     7861 ttcgtaaacg gtacgccaca ggcggcgcag gaagcggtgt gcgccttcga cgccgctgtc
     7921 gctccattcg agggactgtt cgggcggtgc ggcgaacatc ataaacaggc gggcggtgtc
     7981 cgcgccgtag gcgttaatca gttcttgcgg atcgacgccg ttgtttttgg acttggacat
     8041 tttttccgtg ccgctgatga cgacgggcag cccgtcggct ttgaggacgg cggaaatggg
     8101 gcggcctttg tcgtcgaacg tcagctcgac atcggcgggg ttgatccaat ctttgccgcc
     8161 tttgtcgttt tcgcggtagt aggtttcgca aacgaccatg ccttgcgtca gcaggcgttc
     8221 aaacggttcg tcaacattga ctagaccttc gtcgcgcatc agtttggtga agaaacgcgc
     8281 gtacaagagg tgcaaaatcg cgtgttcgat gccgccgatg tattggtcga ccgcgcccca
     8341 gtatttcgcg gcggcaggat cgaccatgcc gtctgaaaat tttggcgaca tgtagcggaa
     8401 gaaataccag ctcgattcca tgaaggtgtc catggtgtcg gtttcgcgtt tcgccgcgcc
     8461 gccgcagcat gggcaggcag tttcgtaaaa ctcgggcatt tttgccagcg gcgaacccat
     8521 gccgtcgggt acgacgtttt caggcaaaac gaccggcaat tggtcggcag ggacgggtac
     8581 gtcgccgcat tgttcgcaat ggacgatggg aatcgggcag ccccagtagc gttggcgcga
     8641 aatgccccag tcgcgcaggc ggtattgggt tttcggttcg cccgcgcctt ggctttgcag
     8701 cttggcggcg acggcgtcga atgccgtctg aaaatccaag ccgtccaagt cgccgctgtt
     8761 gaccaatacg ccgttttctt tgtcgccgta ccattcttgc cattggtttt cgtcaaatgc
     8821 gttgtcgccg acggcaatga cttgtttttt cggcagattg tatttggtgg cgaactcaaa
     8881 atcgcgttcg tcgtgcgccg gaaccgccat caccgcgccg tcgccgtagc cccacaatac
     8941 atagttggca atccacactt ccagcttgtc gccgttgagc gggttgacga cgtagcggcc
     9001 ggtcggcacg ccttttttct ccatcgtcgc catatcggct tcggcaaccg aaccggcttt
     9061 gcattcggca ataaatgcct gcaattcggg tttgtcggcg gctgcggcgg ctgccagcgg
     9121 atgctcggcg gcaacggcaa cataagtcgc acccatcagc gtgtcggggc gggtggtata
     9181 aacttgcagg aatttcgcgt aatcgccttc caagccttgt ttgctgtcgt ctgaaacggc
     9241 gaagcgcacg gtcataccgc gcgatttgcc gatccagttg cgctgcatgg ttttgacttg
     9301 ttccggccag tgttccagct tgtccaagtc gttgagcagc tcttcggcgt aatccgtgat
     9361 tttgaagtaa tacatcggga tttcgcgttt ttcgatcaat gcgccggaac gccagccgcg
     9421 tccgtcgatg acttgctcgt tggcaaggac ggtttggtcg acagggtccc agtttaccgt
     9481 gccgtttttg cgataaacga tgcctttttc aaacagcttg gtaaacagcc attgttccca
     9541 gcggtagtat tcgggtttgc aggttgcggt ttcgcgcgcc cagtcaatcg caaaacctag
     9601 gcttttgagc tgggttttca tgtattcgat gttatcgtac gtccaagcgg caggggcgac
     9661 gttgtttttc atcgccgcgt tttccgccgg catgccgaac gcgtcccaac ccataggctg
     9721 catgacgttg aagccgttta aaagtttgaa gcggctcaat acatcgccga tggtgtagtt
     9781 gcgcacatgc cccatgtgca gcttgccgct gggatagggg aacatggaga ggcaataata
     9841 tttgggtttg gaagcgtctt cggagacgtt gaaaatacgg gcgtcgtccc attttttctg
     9901 cgccgcaggc tcaatggcgg cgggccggta ttgttcttgc atagtcattc tgttttcgct
     9961 taaaaacgtt ggaaaaataa agtcggcatc aattataaca ggttgccgga agcggcgaat
    10021 cggcagattg ccggcaggat gcgtaaattc gcacgcgcat tattccgtat gccgtacaaa
    10081 tacaccgcgt ttattgatac gcacgttttt tatgctaata ttacaaacca aaatcaaatg
    10141 tttaaaactc tcctgatgcg gctcttccga acaaaaggca gacgggcatc gggtaaaaga
    10201 ggattctgca tatgaaaatc aaacaaatcg tcaaaccggg cttggcagta ttggcggcgg
    10261 gcgttctgtc tgcctgcgca accaaaagca acgtcaaagc cgacggcacg accgacaatc
    10321 cggttttccc gaaaccctat tccgtaacgc tcgacaacaa gcgcggcaca ttcccgactt
    10381 atgacgaact ggatcagatg cgccccggcc tgaccaaaga cgacatctac aaaatcctgg
    10441 gccgcccgca ttacgacgaa agtatgtacg gcgtgcgcga atgggattac ctgttccact
    10501 tccatacccc gggcgtaggt atcgaccctg aaaacacttc cggcgtagaa gatgttacta
    10561 cctgccaata caaagtgatt ttcgataaag acaaatttgc ccgcagcttc tactggaacc
    10621 ccgtcttccc gaaagatgcc gcctgtccgc cgcccgcacc caaagccgag ccgcaagtca
    10681 tcatccgcga aatcgtgccg gcaaaaccga aacgtatccg ccaataatcc gacatgccgt
    10741 tccgcctgtt tttagggata ttatgcggcc tgtcaatggt tgcccccgta tatgcacagg
    10801 ggcagccgga tacggtcggc gactttatcc aaaagaaaaa agtcatcgtc gatacatcca
    10861 aagcggaact ctgtttcgct gacgaccgtc agtgccaccc cgtcctcatc ggtgttgcca
    10921 cgcccaaggg gacgttcggg ctgacgctga acagtaccga caagcccgga tacggcggcg
    10981 aagtcatcgg tttcaagcag gagggtgatt ttcttttcgc cctgcaccgc gtttggaatc
    11041 agataccgtc ggaaaggcgg aacgaacgca tcgcctcccc gtccgtgtcc gacaggatta
    11101 tgaccaacgg ctgcatcaac gtcagcgatg cggtgtacga aaaactgcgt cattattttg
    11161 tgttggaagt gatttgaaac agacggatac cgcacgcgcc ggtatctgtt ttcacattgc
    11221 cccgatgcct gaaacagact gtccgccacg tcatgccgtc tgaaaccggc gcagatgccg
    11281 ccaagccttc agacggcatt gcctgcccgc tccgaccgaa caacaaccat ctttgggaga
    11341 accttatgcc cgaacaaaac cgcatcctct gccgcgaact gagcttgctg gcattcaacc
    11401 gccgcgtgtt ggcgcaggcg gaagaccaaa acgtccccct tttggaacgc ctgcgcttcc
    11461 tgtgcatcgt ttcatccaac ctcgacgagt ttttcgaagt ccgtatggcg tggctgaagc
    11521 gcgaacacaa acgctgcccg cagcgcaggc tggacaacgg caaaatgccg tctgaaacca
    11581 tcgccgacgt taccgaagcg gcgcgctccc tgatacggca ccagtacgac ctgttcaaca
    11641 acgtccttca gcccgagctg gcacaagaag gcatccattt ttaccgccgc cgaaattgga
    11701 cagacacaca gaaaaaatgg attgaagact atttcgaccg cgaattgctg ccgatcctga
    11761 cccccatcgg actcgaccct tcccacccct tcccgcgccc gctgaacaaa tcgctcaact
    11821 tcgccgtcga actcgacggc acagacgcgt tcggcaggcc ttcggggatg gcgattgtgc
    11881 aggcaccacg catcctgccg cgcgttgttc ccctgccgtc cgaactgtgt ggcggcggac
    11941 acggcttcgt cttcctctcc tccatcctgc acgcccacgt cggaaaactc ttcccgggca
    12001 tgaacgtcaa aggctgccac cagttccgcc tgacgcgcga cagcgacttg accgttgacg
    12061 aagaagacct gcaaaacctc cgcgccgcca ttcaaaacga gttgcacgac cgcgaatacg
    12121 gcgacggcgt gcggctcgaa gtcgccgaca cctgtcccgc ctacatccgc gactttctgc
    12181 tcgcgcaatt caaactgacc gccgccgaac tctatcaggt caaaggcccg gtcaacctcg
    12241 tgcgcctcaa cgccgtcccc gacctagtca accgccccga tttgaaattt cccacacaca
    12301 cgccgggcag actgaaagcc ttgggcaaaa ccgcgtccat attcgatttg gtgcgccaat
    12361 cgcccatcct gctgcaccac ccctaccaat cgttcgaccc cgttgtcgaa atgatgcgcg
    12421 aagccgccgc cgaccccgcc gtgcttgccg tcaaaatgac gatttaccgc accggcacgc
    12481 gttccgaact cgtccgcgcc ctgatgaagg cggcactcgc cggcaaacaa gtaaccgtcg
    12541 tcgtcgaact gatggcgcgt tttgacgaag ccaacaacgt caactgggcg aagcagctcg
    12601 aagaggcggg cgcgcacgtc gtgtacggcg tgttcggcta caaagtccac gccaaaatgg
    12661 cactggtcat ccgccgcgaa gacggcgtgc tcaaacgtta cgcccatctc ggcacgggca
    12721 actaccacca aggcacatcg cgcatctaca ccgacttcgg cctcattacc gccgacgaac
    12781 aaatcaccgc cgatgtgaac atattgttta tggaaatcac aggtttgggc aaacccgggc
    12841 ggctgaacaa actctaccaa agtccgttta ccctgcacaa aatggttatc gaccgcatcg
    12901 cacgcgaaac cgaacacgca aaagccggca aaccggcgcg gattaccgcc aagatgaatt
    12961 cgctcatcga accgaccgtc atcgaagccc tgtatcgggc aagcgcggca ggcgtacaaa
    13021 tcgatttgat tgtgcgcggt atgtgcacct tgcgcccggg tgtaaaaggc ttgtccgaaa
    13081 acatccgcgt ccgctccatc gtcggcaggc agctcgaaca cgcgcgcgtg tattacttcc
    13141 ataacaacgg cacggacgat acctttatct ccagcgcgga ttggatgggg cgcaacttct
    13201 tccgccgcat cgaaaccgcc acgccgatta ccgcgcccga actcaaaaag cgcgttatac
    13261 atgaaggact gaccatggca ctggacgaca acacccacgc gtggctgatg cagcccgacg
    13321 gcggctatat ccgcgccgca cctgccgagg gcgaatccga agccgacctg caaaacgatt
    13381 tgtggacact gctcggaggc tgacccgcac cgccccaatc aaaaaccatg ccgtctgaaa
    13441 cctttccgtt tcagacggca tggttttaca gcaatctaaa cagggcggac cggagtcaaa
    13501 aacacacctt cgccattcct gcacaagcac ttcccctata cgctcccaac cccaagccgc
    13561 cgcattccag acggcattat agtggattaa att
//
LOCUS       DAWGRZ010000041        13387 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000041 DAWGRZ010000000
VERSION     DAWGRZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13387
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.041"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(111..539)
                     /locus_tag="WHT44_000829"
     CDS             complement(111..539)
                     /locus_tag="WHT44_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ2533040.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(604..2352)
                     /locus_tag="WHT44_000830"
     CDS             complement(604..2352)
                     /locus_tag="WHT44_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ2533041.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2679..4112
                     /gene="ccoN"
                     /locus_tag="WHT44_000831"
     CDS             2679..4112
                     /gene="ccoN"
                     /locus_tag="WHT44_000831"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ2533042.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4139..4750
                     /gene="ccoO"
                     /locus_tag="WHT44_000832"
     CDS             4139..4750
                     /gene="ccoO"
                     /locus_tag="WHT44_000832"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ2533043.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4755..4925
                     /locus_tag="WHT44_000833"
     CDS             4755..4925
                     /locus_tag="WHT44_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ2533044.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4949..6046
                     /gene="ccoP"
                     /locus_tag="WHT44_000834"
     CDS             4949..6046
                     /gene="ccoP"
                     /locus_tag="WHT44_000834"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ2533045.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAVSEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6086..6280
                     /locus_tag="WHT44_000835"
     CDS             6086..6280
                     /locus_tag="WHT44_000835"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ2533046.1"
                     /translation="MPGIPHVGIKADWADRIKKGKDTLHKHAIEGFNTMPAKGGRGDL
                     SDDEVKAAVDYMVNQSGGKF"
     gene            6482..7891
                     /locus_tag="WHT44_000836"
     CDS             6482..7891
                     /locus_tag="WHT44_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ2533047.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYTMDEIHSDMMLGAAGDYLLETAASL
                     TIIMVVSGLYLWWVKRRGIKAMLLPSKGRARSWWRNLHGTFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTSLLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7993..11202
                     /gene="recC"
                     /locus_tag="WHT44_000837"
     CDS             7993..11202
                     /gene="recC"
                     /locus_tag="WHT44_000837"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ2533048.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSALQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRRLG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSEMAGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPKGGNPMWQDVSAWYADV
                     NQTAMFGRFAAFLETLTDIVRIWRQPATVGEWVARCRDLLETLFQAEADDQKSVQNLE
                     NEWVKWQAETELAQFSGQLPPHTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNLIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAEIYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAEMRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLATAEAFAQKQDWEAALKKAQTAYHGSKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTLAAAEKKEEAGEA"
     gene            complement(11270..12838)
                     /gene="mtrF"
                     /locus_tag="WHT44_000838"
     CDS             complement(11270..12838)
                     /gene="mtrF"
                     /locus_tag="WHT44_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ2533049.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAVG
                     AYFGLSVPDPRPVGAKGRADDGLIYIVSLLNADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13170..13382
                     /locus_tag="WHT44_000839"
     CDS             13170..13382
                     /locus_tag="WHT44_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533050.1"
                     /translation="MKNEIQKNMDKYNPWHEDDFESYEDIARDVSLTTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
BASE COUNT         3347 a         3230 c         3554 g         3256 t
ORIGIN      
        1 cactgtaaaa acggagaagg acagaggacg gcagtaaggt ttgccgtcct ctttttcaag
       61 ttgcggtgga atgccgcttg aaggtgtggt ttaaacgttt cggcttgtaa tcagacgcgg
      121 gcgttgtcca gccgctctct gaagttttcc aaatttacgc ctgccgcttg ggcgcggcgg
      181 atgatttcgt caaccggcat accttctgcc gcccggcgga agccccacag gagggagcag
      241 cgcgtaccgg tccggcaata ggcaaggacg ggatattcgg cttgtccgat gagttggcgg
      301 aaggtttcga catcgtgttt ttggatgtcg cgtgcggtaa cgggttggtg atggaatcca
      361 gtaacgcctg cttgttccag ccactgtttg atttgggcga agtcgggttg cgattcttct
      421 tcgcggtcgg ggcggttgca gatgacggtt ttgatgccca gttgcgcgat ttgttccgca
      481 tcggctttgg tcagttgcgg agaaatatag aggtgttcgt caagtttcag aatggccatt
      541 ttgtttcctt tactttgcgg ttgtttttca gacggcattt tgtgtgcaat gccgtctgaa
      601 agtttaaccg aacaaccagt tgtccaacag gttccggtct gtgatatgta ggatggcagt
      661 atccgctgcc gctgcctgaa ccgtcaagac ggtttgacgc aatatgccgg cacggaagaa
      721 gtggatattg atttttgcat tgacgtggta tcgggcccat tgtgcggtaa agtcggtgca
      781 ggcataaccg tctaaagcaa tgattttgtc ttgcgggcac agtgccgcag attccgcgct
      841 gccgccgttg aagacatggg tcaggacgat gtggtcggtg ttttgtttga aacgtgcgcc
      901 aaaatcgcct gccgacggga cggggcagat gtgttctgcg tatccgccgc cgtgttgtcg
      961 gggaagcggc aggaaggtca gtcccacgcc tgcggttgcc aggcattcgg caagcggcaa
     1021 atcttcggta ctgtataacg ctttttggaa aaaatcttcc aaatccaagc cggtaatttc
     1081 ctgacagcgg atttgccagt gtttttccgg aatacccgag tgtgtgtccc tccactcccg
     1141 atagagtttg tccattaacg tatcgagaga atgtctgccg ttgcttcggt tgcgtattat
     1201 cagatcaagg cacaatgcgg caagcgcgcc tttctggtag tagctgacga tggcgttggg
     1261 gctgttttca tccggtttgt aaaatttgtt ccacgcggta aaactcgatt ccgccaaggt
     1321 ctgcctcaaa cggccgcggg tttgttgtac gcgcgtaatg ccttgtgcca gcaggtttaa
     1381 ataagattcg ggcgagatgg tgcggctgcg tgccaaaaac aaatcgtcgt aataggatgt
     1441 aataccttcg aatgcccata gttgttcggt atagttttct ttgtcgaggt cataagggac
     1501 gaacgcggca ggtttgatgg atttgacgtt ccacgcgtga aaatattcgt gggagaaaag
     1561 tccgagcaat gtggtgtagg tatcgtcggc atcggtcata ccgtacggcg gaaggctgtg
     1621 gcggtcggcg agcagggcgg tgctgtcggt gtgttccaaa ccgccgtaaa tatggtcgcc
     1681 gacgtggagc aggaacaaat atttttgaaa cggggcaggg gaggaaaaca ccgccagttc
     1741 tgtttcgcag atttttttga tatccgaaac cagcctgttg cggtcgaaat cgggatatat
     1801 gccgcttaag gcaattgtgt gcggaatgcc tgccgcctca aaatctaaaa attcaatcaa
     1861 gcccatctcg acaggtcggt caatcaattc ggcataagat gccgcctgaa agacaaacct
     1921 ccctgtttcc ggcagcgttg tggcaatacg ccattcggac ggaatacccg tcaattccaa
     1981 gcggtgcggc agcgtttccg ttccttcgac tttcaaaaac aggcacgatc cgtcaaaaaa
     2041 accgcgttct gtcgtcagga aagaacctcg aaccgacaaa tcgaatgcat atacggtgta
     2101 gcggatttgc cactcgccgc gtacggcggc ggcatgccag cggtttttgg caatttgttc
     2161 gagcggcatg gacgtgccgt tacaggatgc atggatagaa gtgatgtggc gggaaaaatc
     2221 ccgaatcaga tagctgcccg gaacccagtt tggcaggctt atttctatag gaagatcatc
     2281 atcttgtgtg aatgtcagca ggatgtgcca ctcgtggtcg agaggggacg gggttatttt
     2341 ataatgaatc ataactatca aatatatcgt taaacgggat ggattaaata cctttaaaat
     2401 gcagcttaac ctacatcaat aaagtgccac aaaaagggaa aattggaaac aaaagcaatg
     2461 gcggtcgggt tgtaatttat tataacaata tgatttatat gtaataaaaa acagattaag
     2521 caaagggctc gtcggcggtg cgaatgtaat tccgcttggc gtaataaggc agatatgtta
     2581 aatttagaaa aaattacgct tgcccggatt gcaccttaaa caggctgcgg aagcggcagg
     2641 ataaaaatct ttattcccat aaattcatgg agactctcat ggacacacaa acttacaact
     2701 acaaagtggt gcgccagttc gccatcatga ctgtagtttg ggggattgtg ggtatgttgg
     2761 tcggcgttat cgtcgccgcc cagctttttg ctcctgccct cgacttgtct aatatcggac
     2821 cttggttcca cttcggccgc ctgcgtccgc tgcacaccaa tgcggttatt ttcgcatttg
     2881 gcggttgcgg cctgatcggc acatcatact acgttgtcca acgtacttgt aatacccgtc
     2941 ttttcggcgg ttggctgccg gcatttacct tttggggctg gcaggcggta atcgttgccg
     3001 ccgtcgtcag cttccctatg ggttggaccc aaggtaagga atatgccgaa ctggaatggc
     3061 cgatcgatat tctgattact ttggtttggg tggcttacgc catcgtattc ttcggtacga
     3121 ttgccaaacg taagattaaa catatttacg ttgccaactg gttctacggc ggctttattt
     3181 tggcggtcgc acttttacac atcgtcaaca atatcagcat cccggccggt ttgatgaagt
     3241 cataccccgt ctattccggt gcgattgatg ctatggttca atggtggtac gggcataatg
     3301 ccgtgggctt cttcctgact gccggcttct tgggtatgat gtactatttc gtacccaaac
     3361 aagcagcccg ccccgtttac tcctaccgcc tgtccgtcgt tcacttctgg gcgttgattt
     3421 ttacctatat gtgggcgggt ccgcaccatc ttcactacac tgcgctgcct gactggacgc
     3481 aatctttggg tatggttctg tctttgattc tgttcgcacc ctcttggggc ggtatgatta
     3541 acggcatcat gaccttgtcc ggcgcgtggg acaaactgcg tacagacccg attcttaaat
     3601 tcctgattgt atccttgtcc ttctacggta tgtctacctt tgaaggcccg atgatgtcga
     3661 ttaaaacggt caatgcattg agccactata cggactggac cgtcgcgcac gttcatgcgg
     3721 gtgcgttggg ctgggtaggc tttgtaacca tcggttccgt ctattacatg attccccgtc
     3781 tgttcggcaa agaacagatg cacagcacca agctggtaga agcacatttt tggattgcga
     3841 ccatcggcgt ggttctgtat atcgctgcca tgtggattgc cggtgtgatg cagggtttga
     3901 tgtggagttc tttgaacgat gacggtacgc tgacctactc gtttgtcgaa tccgtaaaac
     3961 gcaccatgcc ttactacgtg atccgttttg caggtggttt attgtatctg agtggtatgt
     4021 gcattatggc gtacaacgtg taccgcacag ccatcggtgg taaagcagtc gatgccgaaa
     4081 tccctgcggt ttcccaaaca cagcaccact aaaatactaa gaaagatagg ctaccaaaat
     4141 gaaattacaa caattggctg aagaaaaaat cggcgttctg attgtgttca cgctgcttgt
     4201 agtcagtgtc ggtctgttga ttgaagttgt gcccttggcc tttaccaagg cggcaacaca
     4261 gccggcgccg ggcgtgaagc cttacaatgc cctgcaggtt gccggacgcg atatttacat
     4321 ccgtgagggc tgttacaact gccactcgca aatgattcgt ccgttccgtg cggaaaccga
     4381 gcgttacggt cattactctg ttgccggaga gtcggtttac gaccatccgt tccaatgggg
     4441 ttccaaacgt accggtcctg atttggcacg tgtgggcggt cgctattccg acgaatggca
     4501 ccgtatccac ctgctgaatc cccgtgatgt cgtgcctgag tccaatatgc cggcattccc
     4561 gtggcttgca cgcaataaag tcgatgtcga tgcaaccgtt gccaacatga aggctttgcg
     4621 taaagtaggt actccttaca gtgatgagga aattgcgaaa gcacctgagg ctttggcaaa
     4681 caaatccgag ctggatgctg tagtcgccta tctgcaagga ttgggtctgg ctttgaaaaa
     4741 cgtaaggtaa catcatggat attaacggta ttcgtgctct cttcacggta tggatcttta
     4801 tctgtttcct gttggtactc tatatcgtct tcaacaggcg gaataagaaa aactacgata
     4861 atgccgcaaa cagcattttt gatgaaaacc aagatgcgca agataagaaa agcgaaaacc
     4921 gttaatattg tgataacgga gcaaaacaat gaacacaaca tcccaattta ccagtaattt
     4981 ctggaatata tatattgcag ttattgtctt actgagcttt atcgctttgg cttggctgct
     5041 gctgtctcaa aatgttgtca aacgtccgaa gaagggcgaa gaagtacaaa ctacgggtca
     5101 tgagtgggac ggcattgccg aatacgacaa tccgctgccc cgctggtggt tttggctgtg
     5161 tgttttgacg tggctgttcg gtatcggtta tttggttatg tatccgggtg tcggcgacta
     5221 caaaggtctg ctgaaatgga ccagccataa ccaatatgaa aaagaggtca aaaaagccga
     5281 tgagcaatac ggcaaactgt atgccaagtt tgcggatatg ccgattgaaa aagtggcaaa
     5341 agaccctcaa gccaagcaaa tcgcccaaaa cctgtttaac acttattgta tccagtgcca
     5401 cggctctgat gctaaaggct ctaaaggttt tccgaatctg accgatagcg attggttgtg
     5461 gggcggtgat cccgataaaa tccacgagac catcgaaaaa ggccgtgttg cgactatgcc
     5521 tgcctggggt cctgctttgg gcgaagaagg cgtgaaagac gttgcccatt atgtgatgtc
     5581 cctttcaaaa cccgaaggtc agtatgatga ggaacgtgcc gcgcgcggac aagccttgtt
     5641 cagcggtccg cctgccaact gtttcacttg ccacggcgat aagggacaag gtatccaagg
     5701 tttgggtccg aacctgactg atgacgtgtg gttgtggggc ggtacgcaaa aatccattat
     5761 cgaaaccatt accaacggtc gcagcagcca aatgcccgct tggggacatt tcttggataa
     5821 agacaaactg catattatga ctgcttatgt atggggtctt tccgataaag acggtaaagc
     5881 tccggtgaaa aaagccgagc ctgcaccgac tcccgcaccg gcggcagaac ccgcagcctc
     5941 tgctcctgca gaagcagcac aagccgtgtc cgaagccaaa cctgccgcag cagaacctaa
     6001 agccgaggaa aaagctgcac ctgctgccaa agcggacggc aaataggttt atgaaaccgt
     6061 ttgtgccgcc tgccatggca atgcgattcc gggtattccc catgtaggca tcaaagccga
     6121 ttgggccgac cgcatcaaaa aaggcaagga cacgttgcac aaacacgcga tcgaaggttt
     6181 caatacgatg cccgcaaaag gcggtcgcgg cgatttgagc gatgatgagg ttaaagctgc
     6241 ggttgactat atggtcaacc agtcaggcgg caaattctaa cttgactgag tttccgatat
     6301 tatcggttat gaaaatccag attcagtatt gaatctggat tttttgtttt aacgggcatc
     6361 cggttttaaa cgcagactgc ctatttgttg taatgaattt tgactttagt cataaagaat
     6421 tagtctttat tgtttataat cgcgcatcat cacgatagcc ctattttcaa aaggacgatt
     6481 aatggataca caaatcaaaa ctgaagccga caatcagagc aaccggcgtt atctgaccgt
     6541 ttggcggtgg catttttatg ccggtctgct ggttatgcct tttcttaccc tgcttgccgt
     6601 tacgggtttg ggtatgctgc tgtttgccaa tattaccggt aaggagggcg agcggattca
     6661 tgttgtgccg caggcaacgg tacaacctct gtctgttcag gcggaagcgg cacgcagtgc
     6721 cgttaatccg gaaacttcgt ccgtcgttca gtatattgcg ccgcgtgccg atgatatggt
     6781 tgccgtgttc cgtgtcaaca atgagggcaa agcaacgatg gtcgcggtcg atccttatac
     6841 ggcaaaagtg gtcagtacca tgccgcgcaa tcagggttgg tattacacga tggatgaaat
     6901 ccacagcgat atgatgctcg gtgcggcagg cgattatctt ttggaaacgg cagcttcact
     6961 gaccattatt atggttgtca gcggcttgta cctttggtgg gtgaaacggc gcggcatcaa
     7021 ggcgatgctg ctgccgtcaa aaggcagggc gcgttcttgg tggcggaatc tgcacggcac
     7081 gtttggaact tgggtgtcgt tgattttgct gttgttctgc ctgtcgggta ttgcttgggc
     7141 gggtatttgg ggcggcaagt tcgtacaggc ttggagtcag ttccctgccg gtaaatgggg
     7201 tgtcgaaccg aaccccgttt cagtcgtgcc gacccacggc gaggtattga atgacggcaa
     7261 ggttaaggaa gtgccgtggg ttttggagct tacgcctatg cctgtttcag ggacgactgt
     7321 gggcaaagac ggcattaacc ctgacgagcc gatgacattg gaaaccgtcg accgctttgc
     7381 gcgggaaatc ggtttcaaag ggcgttatca gttgaatttg cccaaaggcg aggacggcgt
     7441 atggactttg tcgcaggatt ctatgagtta cgacatgatc agcccgtttg ccgaccgcac
     7501 ggtacatatc gaccagtaca gcggcaaaat ccttgccgac atccgttttg acgattacaa
     7561 cccgttcggc aaatttatgg cggcaagcat tgcgctgcat atggggactc tgggctggtg
     7621 gagcgtgttg gcgaacgtct tgttctgcct tgccgtcatt tttatcggta tcagcggctg
     7681 cgtgatgtgg tggaaacgcc gtccgaccgg agcggtgggc atcgttccgc cggcgcagaa
     7741 agtcaagctg ccggtttggt ggatgatggc attgccgcta ttggcaatcg cactgctctt
     7801 cccgacctca ctgcttgcca ttgccgtgat ttggctgttg gatacgctgc tgttgtcgcg
     7861 gattcctgtt ttgaggagat ggtttaaatg accaaatgcc gtctgaaagg ttcagacggc
     7921 attttgtttg aaggcggacg ggggaaaggc tatataatcc cgaatacttg accacagctt
     7981 ctgtttgaaa tcatgtttta tctgtatcaa tccaaccgtc ttgaaacgct ggcggcattg
     8041 tttgcccgca ttcaaaaagt caaaccgctg aaatcggctt tacagcccga acagattatt
     8101 gtgcagagtc aggggatgcg ccgctacctc aatacctgcc tcgcccgcga tttgggcgtg
     8161 gcggcgaatt tgtcgttcag cctgcccgcc ggcctgacgt ggaagctgat gaaaaaactg
     8221 attcccggta ttccggaact cagcccgttt gcgcccgaag tcatgcgctg gcggctgctg
     8281 gatttgttcc gcagcgaggc atttcggaat acggcagaat ttgaagatgt gaggaatgtg
     8341 ctgcaagact atctgggcag cggcgaatcg gcagattacc agcttgcggg acagcttgcg
     8401 gacatattcg accaatacct cgtgtaccgt cctcagtgga tagacgcttg gcagcagggc
     8461 aggcggctcg gtttgggcga cgacgaaatc tggcagtcca aactgtggcg ttacctcgac
     8521 gacggcaggc agagcgcgcc gcaccgtgtc gcgttgtggg aaaagctgtt ggaatctttg
     8581 agcagtgata agctgcccga gcgttatttc gtgttcggca tttccacgat ggcgccgatg
     8641 tatttgcaac ttttgcacaa gctgtccgaa cattgcgacg tgttcgtgtt cgcactcaat
     8701 ccgagcggga tgtactgggg caacgtcatc gaagcggcgc aaatcctcaa aggtggcggc
     8761 gatcccgatt taactcaggc agggcatccg ctgctcgcct cattgggcaa gcaggggcgg
     8821 gactttttcg actttttgaa cgaaatggaa atagaaggcg aaacgccggt atttgaggaa
     8881 ggcgggcgcg atacgctttt acacgccctg caaaccgata ttcaaaacct gaaaatgccg
     8941 tctgaaatgg cgggaagcgt caacacgggc gacggctcga tacgcatcgt ctccgcacac
     9001 agccctttgc gcgaattgca gatactcaaa gacaagctgt tgaaaattct gcatgaacat
     9061 cccgattggc agccgcacga tatcgccgta ttaaccccga atatcgaatc ctatacgcct
     9121 tttatcgaag ccgtgttcgg acaggcgcag cccggtgcgc aggcattgcc gtattccgtg
     9181 tcagacgtga aaatcagccg ccgccaaccg ctgtttcatg ctttgtcatg cctgttcgac
     9241 ttgttggaaa gccgatttga agtcgataaa gtgcttgtgc ttttagaaac cgcgcccgtg
     9301 ttgcgccgtt tcggactgac tgaggacgat ttaccgcttt tgcacgacat ggttgccgat
     9361 ttgaacgtcc actggggttt ggacggagaa atgcgcggcg gcacggatca gctgttcacc
     9421 tggaagcagg cggtagaacg catgatattg ggctggatgc tgcccaaagg cggcaatccg
     9481 atgtggcagg atgtcagcgc gtggtatgcc gacgtgaatc aaaccgccat gttcggacgt
     9541 tttgccgcct tcctcgaaac cctgacggat attgtacgga tatggcggca gcccgcaacg
     9601 gtcggcgaat gggtggcgcg ttgccgggat ttgcttgaaa cattgttcca agctgaagcc
     9661 gatgaccaaa agtcagtcca aaaccttgaa aacgaatggg tcaaatggca ggcggaaacc
     9721 gaattggcgc aattttccgg acagttgccg ccgcacaccg tcatccgcca tatccgacgc
     9781 tttctcgaca gcgaaagcga ggcaggcttt ttacgcggcg gcatcacctt ttgcagtatg
     9841 gtgccgatgc ggagcctgcc gttcaaagtc atctgcctgt tgggtttgaa cgacggagat
     9901 tttccccgta ataccaaagc cgccgtattc gacctgattg ccaaacatcc cgccaaagga
     9961 gaccgcgccc gccgcgatga cgaccgctac ctgttcctcg aagccctcat cagcgcgcgt
    10021 gaaatcctct acctgtccta catcgggcgc gacatccgca aagacgaaga gcttgccccg
    10081 tcttccctgt tgggcgaact catcgatacc gttgccgcta tggcgggcat cggtagccgc
    10141 caacttgcac aaaacctgat agaacagcat ccgctgcaag ccttctcgcg ccgatatttt
    10201 caagaaggcg gacgttcaga cggcatattc ggcacgcgta ccgactacgc cgccgcgctc
    10261 ggacaaacgc ccgaaccgcc gcaacccttt ttcgatcaac ccgtagaaaa cgccgaacct
    10321 gttgccgaaa tcggacagga cgaatttatc cgtttctggc gcaaccctgt caaagtatgg
    10381 cttcagcagc agcttgcgtg gagcgaaccc catatcggcg aagcctggga gcctgccgaa
    10441 cccttcgagc cgcaacacgc cgatcaaatc gccgaaatct acatcgaagc acggcgcgaa
    10501 ggacgggatt ttgcccaaac cgccgcccgc atcggggcgg aaagcctcct gccgtcggga
    10561 gagttgggca gactttggca gcaggacttc caaactgctg ccaaacaaat cgacacggcg
    10621 gttttaaaca gccccaaact gccaccgctt tcatatgcca taccgtcgga cgggcaaatc
    10681 ctgaaaggca gcttgggcaa tctgtaccgc tgcggacaag tgttttacgc ctacggcaaa
    10741 cccaacgcgc cgcaacgtat cgcttttctg ctggaacacc tgatattttg cgccgttatg
    10801 ccgtctgaag ccgaaatgcg gcaaaccttt atcgtccaat ccggagaaac cgaaatattg
    10861 gcggaaatcg cgcaagacag ggcattgcag ctattgtcgg aatggatggc gtttttcaat
    10921 atcgggcaaa accgcccgct gccgtttttt gccaaaacct cgcttgccac cgccgaagcg
    10981 tttgcccaaa aacaagattg ggaagccgcc ctgaaaaaag cccaaaccgc ctaccacggc
    11041 agcaaagtca gcaaagggca gaaagactat accgaagtcg ccctcgtgtt cggcaacgca
    11101 agccaaaacc cgctcgaaca gcccctgttt gaaaacctcg tccgtctgct tgccgacacg
    11161 cttgccgcag cggaaaaaaa ggaagaggcc ggagaagcct gacgggatag aaaggcaaat
    11221 gccgtctgaa agcgtttaag catttcagac ggcattttgt ttatcgtgtt taaggtgcgg
    11281 gatagaatgt gggcgcgccg ggaccgacgg gcaggcccaa aacaaatacc caaatgcaga
    11341 ataaggcaat ccacgcaatc aagaagaaag cggaatacgg caacatcata gaaatcagcg
    11401 tacccacgcc cgcatctttt ttgtatttga tcaccgtcgc cataatcagc ccgaaataac
    11461 tcatcatcgg cgtaataata ttggtaacgg aatcaccgat gcggtaagcg gcttgaatga
    11521 cttcgggcgc gtagccggcc aacatcagca tagggacgaa aatcggcgca gttaccgccc
    11581 attgcgcgga ggcggagcct atcatcagat tgataaaagc acaaattaaa ataaaaccga
    11641 taaacaacac gctgccgccc aagccgactt cttttaagaa cgtcgcccct ttaacggcaa
    11701 tatattgccc aatattcgtc caattaaaaa atgcgacaaa ctgtgcggca aaaaagatga
    11761 tgaccaaata aagccccaga gtactcatcg attcggccat cgcattaacg acttcctgtt
    11821 cgccgcgcaa acttcgggtt acccggccat aaacaatgcc cggcagtgca aacaacaaga
    11881 aaataaaaac aacaatcgat tttaaaaacg gcgaaccgga aaccaatcct gtttcaggat
    11941 gacgcaaaat accgtcggca gggacgatgc tccaagccaa tagggcggat aaggcaacaa
    12001 acaccacgcc agcccaaatt aatcctttat attccaaagg cgtgatttca ttggaatgcc
    12061 gaatgtcttt ttcttcttgt gacaaatctg attgataagg gcccaattgc ggttcgacga
    12121 ttttttcagt aacaaaataa ccaatcaaag caatcacaaa cgtactggct accataaaaa
    12181 accagttggc ttcagggcct acgacgtagt cgggatggat gatttgcgcc gcctgttggg
    12241 tgatgcctgc caagagcgga tcgattgtgc ctaagaacag attggccgaa taaccgcccg
    12301 aaacgccggc gaaagccgca gccagaccgg caagcggatg gcggccgagg gaatgaaaga
    12361 tgatggcgga caaagggatt aggacgacat agcccaattc agaagcggta ttagataaaa
    12421 tccctgtaaa aacaaccata aaagtagtga gtttgcgtgg cgattttgtg agcaataagc
    12481 gcattaatgc ggaaatcaag cccgattttt ccgcaatccc cacgcccaat aaagaaacca
    12541 acaccgttcc caacggcgcg aaaccggtga aatttttaac ggtatgcgtc aggattttga
    12601 tcaaaccgtc ggcattgagc aggctgacaa tgtaaatcaa accgtcatcg gcacgtcctt
    12661 tcgcaccaac agggcgcgga tcggggacgg atagtccgaa atacgcaccg acggcagagg
    12721 caatcagcaa taacacaatg aaaataataa aaagcgtaac cggatgcggc aacatattgc
    12781 ccagccattc gactgtgcgt aaaaatcgtc cgtcccgttg cgtatcggtt tgactcatct
    12841 gcttctcctt aaaaaaatat attgagtgca ttaaattttt gaatgtaaca tgaagtgttt
    12901 tgtaagcaaa ctaaaataat cgcatatgta tataaccgca atagattaaa acagaaaatg
    12961 cccacatcgt tattctcata aaggcggtaa tccgaaaact tgacgttaaa gccttatcag
    13021 gaatgactgg aactcaaaaa acggtttccc gttttcgcgg aaatggcggg attttcagtt
    13081 tttatttgca aaaatgctgg agaaaaatgc agtttacatt ttgaatggta ttgttattaa
    13141 ttgtaaataa atattggaag gtaaatgata tgaaaaatga aattcaaaaa aatatggaca
    13201 aatataatcc ctggcatgaa gatgattttg agtcgtatga agacattgcc agagatgtat
    13261 cgctgacgac agataaaacg ttcattgaac attatttgtt agaagtttat tcagaagaaa
    13321 acggacattt tgaccaagag aatgtccatg caatgataga agaaattaaa aatgcaattt
    13381 agtgaat
//
LOCUS       DAWGRZ010000042        13109 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000042 DAWGRZ010000000
VERSION     DAWGRZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13109
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.042"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            389..1105
                     /locus_tag="WHT44_000840"
     CDS             389..1105
                     /locus_tag="WHT44_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ2533051.1"
                     /translation="MTMHETTDRLFEIAKEQGVLKPADIAERLNISQQALKNWESRGI
                     AAKALPEVAKAFGVSETWLRTGEGSRTAPVLIDPDLPHEVKDIHRPMTWSSNDPLPDD
                     DYVFVPYLKESCFKGGVGTYEIPDYNGYRLPFGKSTLKRKGINPDNVFCCTLTGDSME
                     EKIAEDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYGDE
                     NAPLDSLTVIGRVFWWSVLD"
     gene            1208..1633
                     /locus_tag="WHT44_000841"
     CDS             1208..1633
                     /locus_tag="WHT44_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533052.1"
                     /translation="MNFLGVQGELKVHELHHRTATLCFEWLGEVSAPRRKENYKDLKP
                     NVLYDFDGSGKHFANPDARYLLPVGSSGLILKHIQIDDEDTLLRLWCARNIPMPHRLS
                     KIPMLRQYYLSKAWHEIYAINQHLRKTKLIVDVAYDPTD"
     gene            1877..2077
                     /locus_tag="WHT44_000842"
     CDS             1877..2077
                     /locus_tag="WHT44_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533053.1"
                     /translation="METITIPKENFDALLNLARTAVALNENFIPKINYGASTIPAAGF
                     AALNEFGIARSTVESLEILKIK"
     gene            2145..2504
                     /locus_tag="WHT44_000843"
     CDS             2145..2504
                     /locus_tag="WHT44_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533054.1"
                     /translation="METQEKKFVPASKQIPTVNSGWLDRELGEAMSEAVRACLAHGKQ
                     SEITVKLKIQPQNIQSGTVKISHDVATKLPKEKREGGIVFATPDGNIQADDPAQGKLK
                     LKQVSNTSSTLKMVRSN"
     gene            2529..3383
                     /locus_tag="WHT44_000844"
     CDS             2529..3383
                     /locus_tag="WHT44_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HEZ2533055.1"
                     /translation="METQENMIKTALQAAQKPFFEFAPNNTPLVFTPDQDGGWRYKSH
                     PELMQNPYRKCGKFLMHDTASLIKFVQKHKQDGTQIYIDADFKSGRIDVTAVINGHTQ
                     QAPGWLDFYAVYTPQHTTSASNWLNNNAHRMNQMEFSHFLTNNARNIVSKNPGNENSV
                     YPTAAEVLDFALNLEYTEKTTFKQGYREQDGRINFTFQSEDSGQTEKNLKMFERFGIE
                     FTPHQGGASYFVEALLKFRIDKNSGALVLWYELQQIDAVIEQAAKDISEAVQKAFPEI
                     DIYFGVIA"
     gene            3453..3668
                     /locus_tag="WHT44_000845"
     CDS             3453..3668
                     /locus_tag="WHT44_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533056.1"
                     /translation="MTITEEYSIDIRVTSEQGKNDYGYPTERYGCDIINTDGELLVGI
                     EPEYKTPFAAVRKALICLTKDNLKNAA"
     gene            3703..3963
                     /locus_tag="WHT44_000846"
     CDS             3703..3963
                     /locus_tag="WHT44_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533057.1"
                     /translation="MKYLIRTALLAVAAAGIYACQPQSEAAVQVKAENSLTAMRLAVA
                     DKQAEIDGLNAQIDAEIRQREAEELKDYRWIHGDAEVPELEK"
     gene            3960..4253
                     /locus_tag="WHT44_000847"
     CDS             3960..4253
                     /locus_tag="WHT44_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533058.1"
                     /translation="MNFQEENENIKNDIEINLLHSAEADSVEAMMDLAVYGLAAMVAK
                     HNKTRGEQLVLQAIAGHMRKLLAALPETESLVQTGKVYRRLEDLIFKTYLPEQ"
     gene            4256..4447
                     /locus_tag="WHT44_000848"
     CDS             4256..4447
                     /locus_tag="WHT44_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533059.1"
                     /translation="MNTNQDTIPFGGNLVICCSTGNGGDGRFSCLITDQIPDLNLVRS
                     GQAFPMFIYEYETREKRKA"
     gene            4447..4587
                     /locus_tag="WHT44_000849"
     CDS             4447..4587
                     /locus_tag="WHT44_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEZ2533060.1"
                     /translation="MDTLLKIIIALSFSGAEALAVWLFITAADAVLRRFQKPSHYRRV
                     KK"
     gene            4584..4793
                     /locus_tag="WHT44_000850"
     CDS             4584..4793
                     /locus_tag="WHT44_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEZ2533061.1"
                     /translation="MNINELGARIDRPTIRELIAYATCRNRPISNSTLLRMEKDGRIP
                     CRLKTPLTSPVWDTREVLEALGLQQ"
     gene            complement(4851..5768)
                     /locus_tag="WHT44_000851"
     CDS             complement(4851..5768)
                     /locus_tag="WHT44_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HEZ2533062.1"
                     /translation="MNVSVLNFGNTPVSFRQDGFLNATAIASHFGKLPKDYLKSEQTQ
                     QYISALAENLSVRRKILTEANQIVIVKRGGSEQGTWLHPKLAIHFARWLNPKFAVWCD
                     EQIEILLNGKISDGIKTVTPKPTRALPDGLTGEQIEAVKKLHNALTKSAPKEAQARIA
                     ITLWSAVKSKFGCSYKEVPAEQFPEVLSVMGRVAVENGVLYGEVLDREPLPAPQPALP
                     ISGNALYDLAVAVRYGAWAIQMGRDVSLPLKQLGCKQAVTMWTVWAETRSRLKAAANA
                     LEALNAHADAEHAAKIRPMLPEIRNLSSV"
     gene            complement(6637..6993)
                     /locus_tag="WHT44_000852"
     CDS             complement(6637..6993)
                     /locus_tag="WHT44_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEZ2533063.1"
                     /translation="MAERNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAI
                     LTVLNQREFVCAGVLGGALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQE
                     FEAVARKWKKKPLKTK"
     gene            complement(7541..7921)
                     /locus_tag="WHT44_000853"
     CDS             complement(7541..7921)
                     /locus_tag="WHT44_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ2533064.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKLV
                     TVVPLSSTEPVPLADYHHKMSGNPLPDKPHIQCWAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAIKTAVAKAFKLY"
     gene            complement(8034..9106)
                     /locus_tag="WHT44_000854"
                     /pseudo
     CDS             complement(8034..9106)
                     /locus_tag="WHT44_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(9297..9386)
                     /locus_tag="WHT44_000855"
     tRNA            complement(9297..9386)
                     /locus_tag="WHT44_000855"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9350..9352),aa:Ser,seq:cga)
     gene            complement(9462..9552)
                     /locus_tag="WHT44_000856"
     tRNA            complement(9462..9552)
                     /locus_tag="WHT44_000856"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9516..9518),aa:Ser,seq:tga)
     gene            complement(9635..10414)
                     /gene="yaaA"
                     /locus_tag="WHT44_000857"
     CDS             complement(9635..10414)
                     /gene="yaaA"
                     /locus_tag="WHT44_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ2533065.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWNTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QASSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            complement(10677..11864)
                     /gene="dapC"
                     /locus_tag="WHT44_000858"
     CDS             complement(10677..11864)
                     /gene="dapC"
                     /locus_tag="WHT44_000858"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ2533066.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTVDADNEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRMYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLALARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVS
                     LYR"
     gene            complement(11942..12394)
                     /gene="dut"
                     /locus_tag="WHT44_000859"
     CDS             complement(11942..12394)
                     /gene="dut"
                     /locus_tag="WHT44_000859"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ2533067.1"
                     /translation="MNIEVEMKVLDERMADVVPVYATEGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVVVPIVQAGFKRVEEFVGSSRGEGGFGSTGSH"
     gene            12594..12959
                     /locus_tag="WHT44_000860"
                     /pseudo
     CDS             12594..12959
                     /locus_tag="WHT44_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
BASE COUNT         3560 a         3366 c         3005 g         3178 t
ORIGIN      
        1 atattaaaaa ttatcgtcat caatgcggct tccccagccc gcggcgagct ttccacgaca
       61 ggcttttttt atttgggttg gtcgtctggt ttctttgagt tcaaaaacag gtcgggatac
      121 ttaagtttta ttcgtgcagg tatcccgcgc ttcgtccaat tgaaaacgca ttgagggcta
      181 ttccctgtta ttcgaccaac ttccgcgtaa ctgccgattg attgcaacag gcgtttgtct
      241 tcattgactc ttttatccat aaataaacca aatgtttaaa tctaatgcta atattaaaca
      301 ctatgtttag ataaaaatca agtcttgtgt aacaacattt tgtttaaata tgggagaata
      361 attaagccaa ccgcgaataa gattaaaaat gacaatgcac gagacaactg acagactttt
      421 tgagatagcc aaagagcagg gagttttaaa gccggctgac atagcagagc gtctgaatat
      481 cagccaacag gctttgaaaa actgggaaag tcgtggcata gcggcaaagg cgctgcctga
      541 agtagcaaaa gcattcggtg tatctgaaac atggctgaga acaggtgaag gcagccgaac
      601 cgcgcccgtc cttattgacc ccgacctacc ccacgaagtc aaagacatcc accgcccgat
      661 gacgtggagc agcaacgacc cgctgcccga cgatgattat gttttcgtcc cctacctcaa
      721 agagagctgc ttcaaaggcg gagtaggcac gtatgaaatc cccgattaca acggctaccg
      781 cctgccgttc ggcaaatcca cgcttaaacg caaaggcatc aatcccgaca acgtgttttg
      841 ctgcaccctg accggcgaca gtatggagga aaaaatcgca gaagacgcgg caattgccgt
      901 agatacgggc gaaaccgcca tacgcgacgg caaaatctac gccttcgccc aggacggtat
      961 gttccgcgtg aagtacctga tacggcagcc tggcaacagc gttctgatac gcagccacaa
     1021 cagcggtttc tatggcgacg aaaacgcccc cttggacagc ctgaccgtta tcggcagggt
     1081 attttggtgg agcgtgctgg attgaaaaat gttgctatta cgacgagata atattgatag
     1141 ggctttcaag attgtcaaaa ataggcgttt tgattcccct tggtggcctg gtgagtacga
     1201 tgccggtatg aatttccttg gcgtacaagg agagttgaag gttcacgagc tgcatcacag
     1261 aacagcaacc ctgtgctttg agtggcttgg cgaagtatct gcgccgcgca gaaaagagaa
     1321 ctacaaagac ctcaagccaa atgtattgta tgactttgat ggttccggta agcattttgc
     1381 caaccctgac gcaaggtatc tattgcccgt cggttcaagc ggcttaatct taaaacatat
     1441 ccaaatcgat gacgaagaca ctctgttgcg gctatggtgc gcacgaaata tcccaatgcc
     1501 acaccggcta tctaaaatcc cgatgcttcg gcagtattat ctaagcaagg catggcacga
     1561 aatttatgct atcaaccaac acctaaggaa aacgaagctg atagttgatg tggcatacga
     1621 ccccacagat taaacaaccc gcccgcatta tgcgggcttt tttcatgccc cgccgaacct
     1681 gaaaacaaca caaaaccgac aaagccgcct taataggcgg tttttttgcg cctgcgaaat
     1741 ctcaaaaata aattctttta ataatcaaaa tgtttataaa ccttaaacaa aaatcttaaa
     1801 cagattgttg catattatta aacgctatgt ttaaatacac ccatcgaaac aaacaacagc
     1861 cacatggagt accgacatgg aaaccatcac catccctaaa gaaaatttcg acgctttgct
     1921 aaatctcgca agaaccgccg tcgccctgaa cgaaaatttt attccaaaaa taaattacgg
     1981 agcatccacc ataccagcag ccggctttgc cgcacttaac gagtttggaa tagcccgctc
     2041 caccgtggaa tctttggaaa ttctcaaaat taaataacct cctcggtatt gtcatcccgt
     2101 gataccggca acctaactaa cctctcttta aaggaaaatc aaaaatggaa acccaagaaa
     2161 agaaatttgt tcctgcctcc aagcaaatac cgaccgtaaa tagcggctgg ttagaccgtg
     2221 aattaggcga agccatgagt gaagcggtgc gtgcctgcct tgctcacggt aaacagtcag
     2281 aaattactgt caagctcaaa atccaacccc aaaacatcca aagcggaacg gtaaaaatca
     2341 gccacgatgt agcaaccaaa ctgcccaaag aaaaacgcga aggcggcatc gtctttgcta
     2401 cacctgacgg caacatccaa gccgacgatc cggcacaagg aaaattgaaa ctcaaacaag
     2461 tttccaatac gtcaagcacg ttgaaaatgg tcagatccaa ctaactcaaa cacaaaagga
     2521 aatctaaaat ggaaacccaa gaaaacatga ttaaaaccgc cttacaagca gctcaaaaac
     2581 ctttttttga gtttgcaccg aacaatactc cgcttgtatt cacaccagac caagacggtg
     2641 gctggagata caaatcacac cccgaattga tgcaaaaccc gtaccgcaag tgcggcaaat
     2701 tcctcatgca cgataccgcc agtcttatta agtttgtaca aaaacacaaa caggacggca
     2761 cacaaatcta cattgatgcc gatttcaaat cagggcgtat tgatgtcacc gccgtcatca
     2821 atggccatac gcaacaagct ccgggctggc tcgattttta tgccgtatac accccgcaac
     2881 acactacatc ggcatccaac tggctgaaca acaatgcgca ccgcatgaat cagatggagt
     2941 tttcccattt cctgaccaac aatgcccgca acatcgtatc caaaaatccc ggtaacgaaa
     3001 attccgttta cccgaccgcc gccgaagtat tggatttcgc gctcaatctc gagtacaccg
     3061 aaaaaaccac cttcaaacaa ggctatcgtg aacaggacgg cagaatcaac tttaccttcc
     3121 aatccgaaga ctcagggcaa acagagaaaa acctcaaaat gtttgaacgt ttcggcatcg
     3181 agttcacccc gcatcaaggc ggtgcatcct actttgttga agccctgttg aaattccgca
     3241 tcgacaaaaa cagcggcgca ctcgtcctgt ggtacgaatt gcagcaaatc gatgctgtaa
     3301 tcgaacaagc ggcaaaagac atttccgaag ccgtacaaaa agccttccct gaaatcgaca
     3361 tctatttcgg cgtaatcgcc taaaaaaaag tcgtctgaaa tcgtgaacct tcagacgacc
     3421 tccacaaaaa cccaaatccg aaagacccca aaatgaccat cacagaagaa tactccatcg
     3481 acatccgagt aacctcagaa caggggaaaa acgactacgg ctaccccacc gaacgctacg
     3541 gatgcgacat catcaacaca gacggggagc tacttgtcgg catagagcca gagtacaaaa
     3601 ccccctttgc cgccgtcaga aaggcgctca tctgtctgac aaaagacaac ctcaaaaacg
     3661 ctgcctgata ttaagccgtt accccaataa tgaggaatca aaatgaaata cttgatccgc
     3721 accgccttac tcgcagtcgc agccgccggc atctacgcct gccaaccgca atccgaagcc
     3781 gcagtgcaag tcaaggctga aaacagcctg accgctatgc gcttagccgt cgccgacaaa
     3841 caggcagaga ttgacgggtt gaacgcccaa atcgacgccg aaatcagaca acgcgaagcc
     3901 gaagaattga aagactaccg atggatacac ggcgacgcgg aagtgccgga gctggaaaaa
     3961 tgaactttca ggaagaaaac gagaacatca agaatgacat agagattaat ctcttgcatt
     4021 ccgctgaagc cgacagcgtg gaggcaatga tggacttggc ggtttacggg ctggcggcga
     4081 tggttgccaa acacaacaaa acgcgcggcg agcagcttgt gttacaggcg attgccggtc
     4141 atatgcggaa gcttttggcc gcgcttcccg aaaccgagtc attggtgcag accgggaagg
     4201 tttaccgccg acttgaagat ttgattttta aaacgtattt gccggagcag taaagatgaa
     4261 cacgaatcaa gatacgattc catttggcgg caaccttgtg atttgctgtt cgacgggaaa
     4321 cggcggggac gggcggtttt cttgtctgat taccgatcag ataccggatt taaatcttgt
     4381 cagaagcggg caggcgttcc cgatgttcat ttatgagtac gaaactagag aaaaaaggaa
     4441 agcctgatgg acaccctatt aaaaatcatc atcgcgctgt cattttccgg cgctgaggca
     4501 ttggcggtat ggcttttcat cacggccgcc gatgccgtat tacgccgctt ccagaaaccg
     4561 agtcattaca ggagagtaaa aaaatgaaca tcaacgaact gggcgcaagg atagaccgcc
     4621 cgaccatccg cgaactgatt gcctacgcaa cctgccgcaa ccgccccatt tcaaactcga
     4681 cactgctgcg tatggaaaaa gacggacgga taccgtgccg tctgaaaacc ccactcacat
     4741 cccccgtatg ggacacccgc gaagttttgg aagccctggg gttacagcaa taaaaaaacg
     4801 cccgattagg gcgttttcac attcagacgg cttttgtatt ccctactgca tcaaaccgac
     4861 gacaggttgc ggatttcggg cagcatcggg cggatttttg ccgcgtgttc cgcgtcggcg
     4921 tgtgcgttta aggcttcgag ggcgtttgcg gcggctttga ggcggctgcg tgtttccgcc
     4981 cagaccgtcc acatcgttac cgcctgtttg cagccgagct gcttcagcgg cagggaaacg
     5041 tctctgccca tttggatggc ccacgcgccg tatctgacgg caacggcgag gtcgtacagg
     5101 gcgttgccgc tgatgggcag ggcaggttgc ggtgcgggca atggttcgcg gtcgaggact
     5161 tcgccgtaca gcacgccgtt ttcaactgcc acgcggccca tcacgcttaa aacttcgggg
     5221 aactgttcgg caggtacttc tttgtagctg catccgaact tgcttttgac ggcagaccaa
     5281 agggtaatgg cgatacgcgc ctgcgcttct ttgggtgcgg atttggtcag ggcgttgtgc
     5341 agttttttga cggcttcgat ttgttcgccg gtcaagccgt ccggaagggc gcgggtgggt
     5401 ttgggggtaa ctgtttttat gccgtctgaa attttgccgt taagtaaaat ttcaatctgc
     5461 tcatcgcacc aaaccgcaaa tttcggatta agccaacggg caaagtgaat agcgagtttg
     5521 ggatgcagcc atgtgccttg ctcactgcca ccacgcttca cgataactat ttgatttgct
     5581 tccgttagga tttttctcct aacgcttaaa ttctcagcaa gtgcagagat atattgttga
     5641 gtttgttcac tttttaggta gtctttaggt aacttgccaa agtgagatgc aatggcggtt
     5701 gcatttaaaa aaccgtcttg acggaaagat acaggggtgt taccaaaatt gagaacagat
     5761 acgttcatga tagtttccta gtttagtttc gaaagaccca aatgggtggt cgggaggttc
     5821 gaaaacctac tagaaaaggc cggcgttatt ccccttgcgg gtattgtatt ccgcaccctc
     5881 ccgaccatag gaaactagtt cggatatatc gaaacaaaca accaaacaag aaaacttatg
     5941 gacgtaaaaa attcacgctg acggggtaaa tgccgttcta gtagaggttt tcgacgcctc
     6001 gtgtttcgta atatatccaa tctttctatt ttctgcaagc ataaaaaagc cgccatgtgg
     6061 cggatagggt cgcggtcttc aacagcatgt aggatttagg aatgcccaaa ttgggcgcag
     6121 gggtgttgaa aacacgcaaa caggcgcgcc gccagcctta cgggtaggcg cacccccaca
     6181 taggagtaaa tcgatttgat acgtatagac gtaaaaaatt ccactctatc gtgttggata
     6241 tacgctgttt gtcaggtgtt ttcaagcacc gtgggaaata ttatatagag ttatggaaat
     6301 gtgtcaagag aattagccca aatgggcgga cacgcggtta agaacccaca taggagggcg
     6361 gacttattcc cctttcgggt cttgtattcg tcgcccacgc ggtcatagaa acttcctgct
     6421 atcgaaacaa acaacaagga aagaaaacta tagacatgaa aaaatcacat tgacggagtg
     6481 attgccgctg tagtgtggtt tctgacgcca cgaacaggaa tataaaacaa aaccccctgc
     6541 acatgcaagg ggtttcccaa aaaaccgtag gcggcaaact gaaaggccgt ctgaatttca
     6601 gacggcctat gttgcggcgg atgttaagct attaagctat ttggttttca gaggcttttt
     6661 cttccatttt cgggctacgg cttcaaattc ttgaaaggcc gtcggtgcgc tcctgatacg
     6721 gcgtatttca aaaataaagc tgcttaggtt gtcccagtca cgtaacagca tggagtattc
     6781 gaaatcttta tacattttct cgtgcagtgc tccgcctaat acgcccgcac agacaaattc
     6841 gcgctggttt aaaactgtca gtatggcttc acggtctttc ttcttgtccg gggtatccga
     6901 tgtataggtg gcgagtatgc atccgtctgt ttttgccagc ccgtttacta tggttatggc
     6961 ttcttgaagg gcggcattgt tacgttcggc catgatcagc gcgctttttg gcgttttctg
     7021 tattcctcat gatgccgtaa gcggcaacaa aaacgccgat aacagtcaat atcggcgttg
     7081 ctatctgtat taggttgtca gtcattgccg tcccagccgt ctgaaaggcg gaaatcacgg
     7141 ctttccgaat aaatatgtcc cattgtcatc cccttaagct gatgttgaaa ataaatcctt
     7201 gcgtcggaat atacggcatt gcttgactat tttcaagatt ctgtacctcg tgaaccggac
     7261 tatacaaaaa aaccgccttc gtgtaaagcg gttcttaaat cgcccgttaa ggcggacaca
     7321 acgatagcca aactggaaca ggaggcggca aaggcgggtt tgtccaaaac agccttgatt
     7381 gaaaggctga ttggggattt gtaggcgttt gcagatacga caaggggcgc attccgcgcc
     7441 ccatcacacg cacggacggc ttgtcatcgc ctgtcgcccg tgcgcggctg cccatatttc
     7501 agaacagtct tgcaagcccc tttaagggaa cggttttatt ctagtacagt ttgaatgcct
     7561 tggcaacggc tgttttaatc gcctgaaaat cctcttcact gattattgga atgcagcggt
     7621 cgcgcccttt gggtttgtat cggtctaatc gtgccaatcc gactgttgcc gtcatgtcgc
     7681 attttgccca acattggatg tgcggcttgt ccggtaaggg gtttccactc attttgtggt
     7741 ggtagtccgc caaagggaca ggttctgtgc tgcttaaggg tacgaccgtt accagtttgc
     7801 cgttgtgcct gtttcgcgct atgacgacga cagggcgttt cttgaccatt tccggttctt
     7861 cataaccgcg aaagtcgcac atgataaccg aacgttccct tggttggaat tttaaaggca
     7921 ttagccgctc ctgacgatga cgggctgcta ttatagcagt tcttacacaa aaaaccgcct
     7981 ttgtgtaaag cggttgcaaa aaagccttcc aataaaaatg cggaaacggt tttttatttg
     8041 cgtttccgca taatactatc caccttcaga acctcgcgca aataagtacg cgcaagcgag
     8101 cgcaaaccgt gcgccgtcgt atccagcccc gcccgctgca tcgccacgcg cggactttcc
     8161 ttattgatat gcccttgaaa attcacactt tcaaacacaa accgcccgtt cacattcaac
     8221 gcccgcgcct gatccaacac ccactgcatc gcccgcgaca gcggcacatc atgcccccgc
     8281 cgctcctttt tccgttcggc ggggatacgc caaacctccc cgtccaactc cgaccactcc
     8341 atcaacgccg cctcctgaat ccgcgtcatt gtcaacagca gccaatagat acaaagccgc
     8401 gttacaggat ggatacgcac cctgcccgca taaaccgcaa attcattgcg ctgctccaaa
     8461 aaatcaatca ggcgcggcaa ttcagacgga ctcaacgctg ccatattccc cgttttcgcc
     8521 cgttcaaaca cacccgaacc gatttgcgcg acagggttga tttccatcat tcccgaaccg
     8581 accgcaaacg caaacaccat cttcagactg tttttcgttt tgcgcaacgt atcaacgata
     8641 ccgcgcgcct ccatcacacg cagacagccg accacatccg ccgtcctgat ttgacgaata
     8701 tcaagattgc cgatagccgg aaaaacccac cgctcaaaat tccgcataac ctgtccggca
     8761 tacttttcag accgcccctt cgaccaacgc acaaaccaat cacgcgccac cttctcaaaa
     8821 gcaaaatccg cccgcacctt cttattgacg acattttccc cgtgcgcccg ttttcggcgc
     8881 acctcctccc gccattcccg cgcatcggcc agcgaaaaat caggataccg ccccagcgaa
     8941 attgtctgct gccttccatc ctgcacaaac gacagcttcc aactccgccc gcccgtcggg
     9001 tagacccaca aagccagccc gccccgtctg acaacttata cagcttatcg cgcggcttcg
     9061 catttttaac ctgatttgcc gacagcggcg taatgatttt cgccattatg gtaatttccc
     9121 atttatccaa aaattaccat acacattacc ataaaaaaat gcaataagaa gatgccgcaa
     9181 gattgcaaat gatttgcttt gatgcgttat gatagggcaa accgatgatt tttatatggt
     9241 aattgataca acttgaacaa aaaagaaccg ccccgaatca gggcggtttt gttttgtggc
     9301 ggaaacggtg ggattcgaac ccacggagga tttgcaccct cagcggattt cgagtccgct
     9361 gcattcagcc tctctgccac gtttccgata atgcagtaaa accaaataaa aatacaatat
     9421 ttgcggctat tgcattctta tttggtttta cgctcaaaaa ttggcggaag cggtgagatt
     9481 cgaactcacg gagggctatc aaccctcgac ggttttcaag accgttgcat taaaccactc
     9541 tgccacgctt ccgtcttctt gaagattagg aataataatg aaatttatac attttgccaa
     9601 gcactttttt cggaaaatac ttaatttatt gttttcactt tatttgttcc gaacgcataa
     9661 aaacccattc gctttcattt gaagccgcgt cattgaatgc gtagccttcg taattaaaat
     9721 ttttcagcat ttcagggtcg gtaatatgat gttctgccgc atagcgcacc attaatccac
     9781 gcgcgcgctt ggcatagaaa ctgatgattt tatatttacc gtttttttcg tctttaaata
     9841 ttggggtaat cagccgcgcc cgaagttttt tcgtgttgac ggacttgaaa tattcctgtg
     9901 aggcaaggtt gacaagcgta ttgctgcttg cttgggcaag cgtatcattt aaaaggttgg
     9961 taatgatgtc gccccaaaac tcatacaaat tcttgccgcg caaattggca aatgccgtcc
    10021 ccatttccaa acgatagggc tgtatcaggt ctaacgggcg aagaagaccg tacagaccgg
    10081 acagcaggcg gacatggttt tgcagatagc gtatctgtcc aatatccaat gtgtttgcat
    10141 ccataccttc gtaaacatcg ccgttgaaca taaagaccgc ctgtttggcg ttttccggcg
    10201 taaacggcgt gttccattct gcattgcgct gcgcgtttaa gagggcaatt ttgtcggaaa
    10261 cgtgcatcag ttcggcaatc tgttgcggcg caagctcgcg caactgctgc attagaatgt
    10321 cggactctgc cagcaggtcg ggttgggtaa actcgctgac aggggcaggg tctttttcat
    10381 taaggttctt cgcaggggaa aggacaaaaa acataatctg ctcggtattg tgtcaaacac
    10441 ggggatttta agtttaaacg tgtttgcggg caagccgatt aaaagtgaag tatagtggat
    10501 taaatttaaa ccagtacagc gttgcctcgc cttgccgtac tatctgtact gtcttcggct
    10561 tcgtcgcctt gtcctgattt ttgttaatcc actataaata ccgttatagc aaattcaacc
    10621 gtaaaatatt gaaaaatgcc gtctgaacaa aagttcagac ggcatttttt tattctttac
    10681 cgatataggg aaacaatggt ttccgcagct ttgacacaag ttgcgacatc ggcaaccaaa
    10741 gcgatacgca cataaccttc cccgggattg ccctgttcgg tatcccgcgc caaaaaacgt
    10801 ccgggcaata cttggatagc cgctttttgc cataaattgc gtgcaagtgc caaatcgtcg
    10861 ccatcaggga ctttcaacca gatgtaaaac gaggcatccg gtaatttaac gtcaaatacc
    10921 tgttgcaaaa tgggaataac gcgctcaaat ttttcctgat acatacggcg gttgtcgata
    10981 acgtgctgtt catcatccca agcggcaatg cttgcgcgct gcacgggaat actcattgca
    11041 ctgccgtgat aggttctgta aagcagaaag tttttaagca gttcggcatc gccggcgaca
    11101 aaaccggaac gcaggcccgg aacgttggaa cgcttggaca aactggtgaa cataagcagt
    11161 ttttgcctgc ttcgacccaa ctgtgcagcg gcttgcaggc agcccaaagg tttgttgccg
    11221 tcgaaataga tttcggaata gcattcatcc gaggcaataa tgaaaccata tttatcctgt
    11281 aaatcaaaaa cttctttcca gccgtccaaa tccagcacgc tgccgctggg gttgttgggc
    11341 gagcagacga acaccagttt ggtgcgtttc caaacctctt cggaaatact gcgccaatcg
    11401 gggttgaaag acggcgcggg gcaattggca aaatggattt caccgccgcc caaaagtgtc
    11461 gcaccttcgt aaatctgata aaagggattc gggctgacaa ttgcgggttt gatgccgtct
    11521 gaaacagggt tcaacacggt ttgaacaaaa gaaaacaacg cctccctact gcctaaaacc
    11581 ggcagaattt cattatccgc atccactgtc aagccatcgt aacggcgttt taaccagttt
    11641 gcacacgcct gacgcagttc aggcagaccg gccgtcagcg gatatttttc caactcgtgc
    11701 aatgaggcgg tcagcgcatc cgtaatgact ttcggtgtcg gatgtttcgg ttcgccaatg
    11761 tgcaggggga cggcttccag accttcgggc gcggaaatgc cctgcatcgc ttcacgcagt
    11821 cgggcaaagg gatatggttt gagttggttg agtaaggtat tcatgatttt ctccggttgt
    11881 cggtatatcg gaagatatgc cgtctgaacc tgacgtgtct ttcagacggc attctatatt
    11941 tttagtgaga acccgtactg ccgaagccgc cctcaccccg gctgcttccg acaaactcct
    12001 cgacacgttt gaagcccgcc tgcacgattg gcacgacaac catctgcgcg atacgctcaa
    12061 acggtttgac agtaaacggt tcgctgcttc tgttccataa cgacaccttc aattcccctt
    12121 gataatcgga gtcaatcaaa ccgaccaaat tgcccaaaac aatgccgtgt ttatgcccca
    12181 agccggaacg gggcagcaaa acggcggcat atgcgggatt cgccaaataa attgccaaac
    12241 ccgtcggcac aagaaacgtt tcacccggct gcaaaacgac ttcctcatcc aaacaggcgc
    12301 gcaaatctaa acctgcagaa ccctccgttg catagacagg gacaacatcc gccatccgtt
    12361 cgtccaatac tttcatttct acttcaatat tcatcgtctt gctcctgcat acggccggtt
    12421 tcaaagatgg gagattatac acaagccgtg tttacgctga aacacggcaa atcggttttg
    12481 ttttgaaatc cgttgccagt gtataattcc cgcccttccg gttatgaaga cgaacattat
    12541 gcacaccttc cccgcataac gaatttatac gcggcatcaa agaaagttcg cctatgctga
    12601 ttgggctgct gccttgggca ttaatactcg gtatgcaggg cggacaaaaa ggcatgagct
    12661 ggctggaaat gttgttgatg accagtatga acttcgccgg cggctccgag tttgccacgg
    12721 tcaacctgtg ggcggaacct ctgccgatac tgcttatcgc caccgtaacc tttatgatta
    12781 attctcggca tatcctgatg ggggcggcgc ttgccccgca cctgaaagga ataccgctga
    12841 aaaaagccgt gcccgcactg ttttttatgt gtgatgaaag ctgggcgatg gcattctccg
    12901 aaatccaaaa acggaaagca gccggtttgc ccgcattcaa tatgcctttt tatagtggat
    12961 taacaaaaac cagtacagcg ttgcctcgcc ttagctcaaa gaaaacgatt ctctaaggtg
    13021 ctgaagcacc aagtgaatcg attccgtact atctgtactg tctgcagctt cgccgccttg
    13081 tcctgatttt tgttaatcca ctatatgaa
//
LOCUS       DAWGRZ010000043        12231 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000043 DAWGRZ010000000
VERSION     DAWGRZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12231
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.043"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            243..980
                     /locus_tag="WHT44_000861"
     CDS             243..980
                     /locus_tag="WHT44_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ2533068.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLSNRTVAD
                     NVAFPLEIAGWPSEKIKARVKECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHAQSDIGRELIRED"
     gene            982..1668
                     /locus_tag="WHT44_000862"
     CDS             982..1668
                     /locus_tag="WHT44_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ2533069.1"
                     /translation="MADLTFQQAVSTIVGMKDEIFRALGETFVMVGLSTTFAVIFGTL
                     LGVLLFVTSSRQLHYNKLVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVCKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIIFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            1827..2690
                     /locus_tag="WHT44_000863"
     CDS             1827..2690
                     /locus_tag="WHT44_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533070.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAAKKE
                     IVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(2752..3321)
                     /locus_tag="WHT44_000864"
     CDS             complement(2752..3321)
                     /locus_tag="WHT44_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ2533071.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDVETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            3479..4339
                     /locus_tag="WHT44_000865"
     CDS             3479..4339
                     /locus_tag="WHT44_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ2533072.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEVKTANHKKGRIVLYFDTPETFGHL
                     LEQLGIDYRP"
     gene            complement(4535..4972)
                     /locus_tag="WHT44_000866"
     CDS             complement(4535..4972)
                     /locus_tag="WHT44_000866"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533073.1"
                     /translation="MFLRDLFALRGNNKKSTRNCSSLSVGLSAVWGICSINKRSSENI
                     FRRPFPNKGLATACRFKQSIALTFAFVRSASQTNCIGSGNADCADYTGNIALAHALTH
                     SCGKVDAYAFIVAVADFYGIWADAQGKRGTFLKFLVGNGYIID"
     gene            5181..5534
                     /locus_tag="WHT44_000867"
     CDS             5181..5534
                     /locus_tag="WHT44_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ2533074.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            5524..6390
                     /gene="atpB"
                     /locus_tag="WHT44_000868"
     CDS             5524..6390
                     /gene="atpB"
                     /locus_tag="WHT44_000868"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ2533075.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            6447..6683
                     /gene="atpE"
                     /locus_tag="WHT44_000869"
     CDS             6447..6683
                     /gene="atpE"
                     /locus_tag="WHT44_000869"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ2533076.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            6754..7224
                     /locus_tag="WHT44_000870"
     CDS             6754..7224
                     /locus_tag="WHT44_000870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ2533077.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            7229..7762
                     /locus_tag="WHT44_000871"
     CDS             7229..7762
                     /locus_tag="WHT44_000871"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ2533078.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELVQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            7773..9320
                     /locus_tag="WHT44_000872"
     CDS             7773..9320
                     /locus_tag="WHT44_000872"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ2533079.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTCGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            9345..10220
                     /gene="atpG"
                     /locus_tag="WHT44_000873"
     CDS             9345..10220
                     /gene="atpG"
                     /locus_tag="WHT44_000873"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ2533080.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCFGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            10258..11655
                     /gene="atpD"
                     /locus_tag="WHT44_000874"
     CDS             10258..11655
                     /gene="atpD"
                     /locus_tag="WHT44_000874"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ2533081.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRAIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            11666..12088
                     /locus_tag="WHT44_000875"
     CDS             11666..12088
                     /locus_tag="WHT44_000875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ2533082.1"
                     /translation="MSIMQVEVVSSEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDRARAEEAKKAA
                     EAGISQAKDDKALAEAHKVLAAAIAQLKTLDYIRSHKK"
BASE COUNT         2997 a         3129 c         3160 g         2945 t
ORIGIN      
        1 tcaattattt gccgttttgg tgcgaaccgc tgcctttgcc cgtttcagac ggcattgtcc
       61 gaaatggttg cccgcttcct gctttattga caaaaaaatg ctttcccgat aatatcctac
      121 gaaaattaac ctgccgattt gacacagctt gcgggcataa cagctaaagc gttccgacaa
      181 tttcagcttt atcttccgcg cccgttgtgt ccgacatcgg gctttgttgt atgggaaaga
      241 caatgattat tttggacaag gtttccaagc attaccaaac gcgcgacaag acccgttttg
      301 ccgccgtcga gccgaccagc ctcgaaatcc gcgacggcga aatcttcggg ctgatgggtt
      361 attcgggtgc aggcaaatcc accctgttgc gcctgattaa cctgttggaa cgccccgaca
      421 gcggcaaggt caacgtctgc ggacaagagc tgaccgcgct cgatgccgcc gcattgcgtc
      481 aggctcggca gaatatcggc atggtgtttc agcagtttaa tcttttgagc aaccgcaccg
      541 ttgccgacaa tgttgccttt cctttggaaa tcgccggatg gccgtctgaa aaaatcaaag
      601 cgcgcgttaa agaatgcctt gaaatcgtcg gcttgaccga acgcgccggc cactatcccg
      661 cccagctttc cggcgggcag aaacaacgtg tcggcatcgc ccgcgcactc gcgcccaaac
      721 cccaagtcat cctcgcagac gaacccactt ccgccctcga ccccgccacc acgcgcagcg
      781 tcttggaatg tttggaagac atcaacaaac gcttcaacgt aaccatcgtc atcgtaaccc
      841 acgaaatgag cgtcatccgc cgcctgtgcg accgcgccgc cctcttggat aaaggcaaag
      901 tcgtcgaaat cgtcgaagta cgcggcaacc aaatccacgc ccaatccgac atcgggcgcg
      961 aactgattcg ggaggactga tatggcagac ttaacattcc aacaagccgt ttccaccatc
     1021 gtcggcatga aagacgaaat cttccgcgcc ttgggcgaaa ccttcgtgat ggtcggcttg
     1081 tccaccacat tcgccgtcat cttcggcacg ctgctgggcg tgctgctctt cgtaacctcc
     1141 agccgccaac tgcattacaa caagctggtg aacttcctgc tcgacaacct cgtcaacctc
     1201 atgcgcgcct tccccttcgt catcctgatg attgcgatga tacccgccac acgcgccatc
     1261 gtcggcagca ccatcggtcc ggttgccgcc tcgctggtgt tgagcgtgtc gggattgttt
     1321 tattttgccc gactggtgga acaaaacctg cgcgaagtcc ccaaaggcgt aattgaagcc
     1381 gccgccgcga tgggtgcgcc gccgattgcc atcgtctgca aagtcctctt gaacgaagcg
     1441 cgcgcgggca tggtttccag cattaccgtg cttgccatcg ggcttttgtc atacagcgcg
     1501 gcggcaggga tgataggcgg cggcggcttg ggcgacctcg ccatccgcta cggctactac
     1561 cgctaccaaa ccgaagtcat catcttcatc gtcgccctcc tcgtgctgct ggtcatcctg
     1621 attcaaagca ccggcaacgc gttggcgcgg aaactcgaca aacgttgaac ccgaatgccg
     1681 tctgaacgcc aaaaccccca ccgctatccg aaaaatgcta taaaatcccc ctgttcgcgg
     1741 caaatgccgt ctgaacgccg aatccggacg gcaggactcc ctgcccgtca tttttgtttg
     1801 aaactgccac aacatcagga gaaaatatga aaaccttctt caaaaccctt tccgccgccg
     1861 cactcgcgct catcctcgcc gcctgcggcg gtcaaaaaga cagcgcgccc gccgcatccg
     1921 cttctgccgc cgccgacaac ggcgcggcga aaaaagaaat cgtcttcggc acgaccgtcg
     1981 gcgacttcgg cgatatggtc aaagaacaaa tccaagccga gctggagaaa aaaggctaca
     2041 ccgtcaaact ggtcgagttt accgactatg tacgcccgaa tctggcattg gctgagggcg
     2101 agttggacat caacgtcttc caacacaaac cctatcttga cgacttcaaa aaagaacaca
     2161 atctggacat caccgaagtc ttccaagtgc cgaccgcgcc tttgggactg tacccgggca
     2221 agctgaaatc gctggaagaa gtcaaagacg gcagcaccgt atccgcgccc aacgacccgt
     2281 ccaacttcgc ccgcgtcttg gtgatgctcg acgaactggg ttggatcaaa ctcaaagacg
     2341 gcatcaatcc gttgaccgca tccaaagcgg acatcgccga gaacctgaaa aacatcaaaa
     2401 tcgtcgagct tgaagccgcg caactgccgc gtagccgcgc cgacgtggat tttgccgtcg
     2461 tcaacggcaa ctacgccata agcagcggca tgaagctgac cgaagccctg ttccaagaac
     2521 cgagctttgc ctatgtcaac tggtctgccg tcaaaaccgc cgacaaagac agccaatggc
     2581 ttaaagacgt aaccgaggcc tataactccg acgcgttcaa agcctacgcg cacaaacgct
     2641 tcgagggcta caaatcccct gccgcatgga atgaaggcgc agccaaataa ggcagtcgta
     2701 taaaatgatg ccgtctgaac tgtatccgtg ttcagacggc atttttgtcc tttaatccgc
     2761 cattccctgc cattccgccg aatccggcgt atcgattccg aacagcgaca aagcgtgtgc
     2821 aacactgtgc gccactatgt cgtccgccgt ctgcggtttg cggtacatcg caggaacagg
     2881 gggaaacacc acgccgccca tttccgttac ccgcttcata ttgtccaaat gggcaaggtt
     2941 cagcggcgtt tcgcgcacca tcagcaccag ccgccgcctt tccttcaaaa ccacatccgc
     3001 cgcacgcgtc agcagattgt cgccgaagcc gtgcgcgaca gaggcaagcg tccgcatcga
     3061 acagggggcg accagcatcc catccgtttt aaacgtaccg ctggcaatgc acgccccgat
     3121 attgccgatc ggatgcacga agtccgccaa ggcatatacc tcgtctctcg cataagccgt
     3181 ttccgaagcg cgcgccatct ccgcaccttt cgataccaca aggtgcgttt cgacatcttg
     3241 cgcgcgcaaa agttccaaag ccttcacgcc gtattggaaa ccgctcgccc cgctgatgcc
     3301 gattatcaaa cgccgtacca tcatccgcct ttcccataaa accgcctgca acggcaaacc
     3361 ggctattata gtgaaaaaac agaaatccga taaacgcgga tacaaattgt cggcaacacc
     3421 caatatccga taaaataccc gatttaacat cctatctgaa taggcacggg agggcggtat
     3481 ggcaaaagta aaaggcggat tggggcgcgg cttggattcg ctgctcgcca acggcgcgga
     3541 caacagcagc ggcgaccgat tgaccacggt tgcggttaaa gatatccggc ccggccgcta
     3601 tcaggcgcgt gttcaaatcg atgacgaagc cttgcaggaa ctggcagatt cgattaaggc
     3661 gcaaggcgtg atacagcccg tcatcgtgcg cgaacacgga ctgtcccgat acgaactgat
     3721 tgcaggcgaa cgccgttggc gcgccgcaca gattgccggc ctgaccgaaa tccccgccgt
     3781 tatcaaaacc atcagcgacg aaaccgcatt ggcaatgggt ttgatcgaaa acctccagcg
     3841 cgaaaacctc aaccccatcg aagaagcaca aggcttgaaa cgccttgccg acgagttcgg
     3901 gctgacccac gaaaccatcg cccaagccgt cggtaaaagc cgaagcgcga tttccaacag
     3961 cctgcgcctt ttaagcctgc ccgaacccgt gcaggaaatg ctttaccaac gccgcctcga
     4021 aatggggcac gcccgcgcat tgctgaccct gcccgtcgtc gaacagctcg aattggcgca
     4081 aaaggccgtc aaaaacggct ggtcggtgcg cgaagtcgaa cgccgcagcc aggccgccct
     4141 tcaaaacaaa cgtcccgagc ccaaaaagac tgccgccgcc gacatcggcc gcctgaatga
     4201 tttgctgact gaaaaactgg gtgtcaacgc tgaagtcaaa accgccaacc acaaaaaagg
     4261 caggattgtc ctgtatttcg atacgcctga aacgttcggc cacctgctgg agcagttggg
     4321 catagattac cggccttaat tttgcgggat ataccgtctg aaatatagag aatagctttc
     4381 cagattttaa gtgggaaata taattctatt gacatttttt ctgcttcacg taagaatcgt
     4441 tttcctgttt tcatttttaa ttttcgagga aattatgaac acacgcatca tcgtttcggc
     4501 tgcgttcgtt gcgttggcat tagcaggttg cggctcaatc aataatgtaa ccgtttccga
     4561 ctagaaactt caggaacgtg ccgcgtttgc cttgggcgtc agcccaaatg ccgtaaaaat
     4621 cagcaaccgc aacaatgaag gcatacgcat caactttacc gcaactgtgg gtaagcgcgt
     4681 gagccaatgc tatgttacca gtgtaatcag cacaatcggc gttaccactt ccgatgcaat
     4741 ttgtttggga ggcggaacgc acaaaggcaa aagtcaatgc aatgctttgc ttaaagcggc
     4801 aggcagttgc taatccttta ttcggaaaag gtcgtctgaa aatattttca gacgaccttt
     4861 tatttattga gcaaattccc caaactgccg acaatccgac cgataaactg ctacaatttc
     4921 gcgttgattt tttattattt ccacgtaaag caaacaaatc acgtaagaac attaaaaatg
     4981 taaagaaatt gcggacaaac tccttaatat cctgtaatta tatgtaatgt gtgttagaat
     5041 gacacctgtc gccgttattc aggcggcgag cgcggcggat tgggatacgt tgtttcacaa
     5101 agattaactg tcgcttgacg taataaggct tctaaattat attagccgcg ccccaaatcc
     5161 gaggcggaaa cattcccttt atgaagcaga ttatcatcct acaatccgcc gtattgtcca
     5221 tatgcgccgc agttgccttt gctgtatggg gttttgccgg attcctttcg gcggtcggtg
     5281 gcggtttgtc ctacctgctt cccacgtttg ttgcagtttt acttttaaaa cttttcaggg
     5341 gaaacccctt cctgcaaagc aggatgttcg tcttcggaga gattttaaaa gtagtgctgt
     5401 cgctgttgtc catgcttgcc gtatttgcaa tatggcatca atcgctggtg ttcgccccgt
     5461 ttctgatggg gctgctcggt gtcagccatt tggttttttt agtattgttg agagtgaaag
     5521 attatggcag gtgaaaccat taccgctgcc gactacatca agcaccactt gcagagcttg
     5581 accagtttgt cggatgttac tcagggtcag ggactgaaaa acattgctga tttttcgttt
     5641 attaaccttg atgccgtctt ttttgccgtc ctgttgggcg taatcggcag ctttctgttg
     5701 tggcgcggtg caaaaaaagc gacggcaggc gttcccggac gtttccaggc cgcagtggaa
     5761 atcttgtttg agtttgtgga cgatatgtgt aagagcatca ttcacaacga aaagtctaga
     5821 aaagccgtcg cgccgttggg tctgacgctg tttgtctgga tttttctgat gaacgcgatg
     5881 gatatgctgc cggtcgattt gctgccgatg gtatggcagg gcattaccgg caaccatcac
     5941 gccctgctgc gcgtcgtacc gactgccgat ttgaacacca ctttggcact cgctgtcggc
     6001 gtgttgctga tttgtatcta ttacaacatc aaaatcaaag gattgggtgg ctggtttcac
     6061 gagttgttca gcgcgccgtt cggtgcaaaa ctcgcacctg cgaactttct gttgaatttg
     6121 gtcgagtttc tttctaaaac cgtatcccac ggtatgcggt tgttcggcaa tatgtatgcg
     6181 ggcgaattgg tattcttgtt gatagccttg cttggcggtg cttgggcggc ttccggcagc
     6241 gtcgaagtca tggatccgat tctgtttgta ttccacatta ttgccggttt ggcatgggcg
     6301 attttccata ttttggtgat taccctgcag gcgtttattt tcatggcgtt ggcgttcgtc
     6361 tatatcggac aggcacatga tgcgcactga ttttcctaag tagtttgttt ttgtagttta
     6421 acctttgttt ctttaaggag tttaaaatgg gtttgattgc tatcgcatgt ggtttgatcg
     6481 ttgcattggg tgcattgggt gcctctatcg gtatcgcaat ggtcggttct aaatatttgg
     6541 agtcttctgc ccgtcagccc gaactgatcg gtccgctgca aaccaaactg ttcctgattg
     6601 ccggtttgat tgatgccgca ttcttgattg gtgtcgccat tgcactgctg ttcgccttcg
     6661 ttaacccgtt tgcaggtgca taatcaggac ggattcgtcc gttccgtaca ggcgcagcct
     6721 gtttgattaa ccccagtacg aaggttaagt aacgtgaata tcaatgcaac attattcgct
     6781 caaatcatcg tctttttcgg tttggtatgg tttaccatga aattcgtgtg gccgccgatt
     6841 gcaaaagctt tggatgagcg tgccgcaaaa gtcgccgaag gcttggctgc cgccgagcgt
     6901 ggtaaaagcg attttgagca ggctgaaaaa aaggttgcag aactcttggc agaagggcgt
     6961 aatcaggttt ccgaaatggt tgccaacgcc gaaaaacgtg ccgccaaaat tgtcgaagaa
     7021 gccaaagaac aggcttcttc cgaggcggcg cgcattgcag ctcaggcaaa ggccgatgtg
     7081 gagcaggaat tgttccgcgc acgcgaatcc ctgcgcgagc aggttgccgt gttggctgtc
     7141 aaaggtgccg aatctatttt gcgcagcgaa gtcgatgctt ccaaacacgc aaaactgctc
     7201 gataccctga aacaggagct gtaatcttat ggcagagttc gcaacgattg ccagacctta
     7261 tgcaaaggca ttgttcggtc tggctcagga aaaaaaccaa attgagtctt ggttgggcgg
     7321 actggaaaaa cttgcggcgg ttgttcagga agggaaggtg gcttcattga ttgaccgtcc
     7381 tgaaacgaat gcttcagaaa aagcagatat cctcatcgat ttggtcggtt tgaaagacaa
     7441 ggagttgaaa aactttgtta tcgtcttggc cgggcagaaa cgtttgtcga tattgccgga
     7501 agtatatgct caatatcaag acttgacctt atcattcaac catatcaaat ctgccgtcat
     7561 ttacagtgcc tatccgttga ccgacaaaca ggtcggcgag ttggtgcaaa tgctgaataa
     7621 gcgtttcgac agcgagctga aaatctctgt cgaaatcgaa ccggagctga ttggcggcat
     7681 aaaagttgaa gtgggtgatc aggttttgga tttgtctgta caaggcaaat tgagtgcctt
     7741 gtacacgact atgacgaatt aggagagttt tcatgcagct taatcctgct gaaattagcg
     7801 atttgattaa agccaagatc gaaaatctgt ctgtaaatgc cgaagtgcgt acctgtggta
     7861 cggtaatttc ggtaactgac ggtatcgttc gcatccatgg tttgtcagat gcaatgcaag
     7921 gtgagatgct cgaattcccg ggtaacactt tcggcttggc gatgaacctg gagcgcgact
     7981 ccgtcggcgc cgtagtgttg ggcgagtacg aacacattaa agaaggcgac acggttacct
     8041 gtaccggtcg tatcttggaa gtgccggtcg gacgcgaact ggtcggacgc gtcgttgatg
     8101 cattgggtcg gcctatcgac ggcaaaggcc cgattaatac aactttgacc gcccctatcg
     8161 aaaaaatcgc accgggcgtg attgcgcgca aatcggttga ccagccgatg caaaccggtc
     8221 tgaaggcgat tgactctatg gttcctgtcg gtcgcggtca gcgtgagttg attattggcg
     8281 accgtcagac aggtaaaacg gccgtagcat tggatgccat cgtcaaccaa aaaggtacgg
     8341 gtgttatctg tatctatgtc gctatcggtc aaaaagcatc ttctattgcc aacgtggtcc
     8401 gcaaattgga agagcatggc gcgatggagc ataccatcgt ggttgctgca actgcatctg
     8461 aagcggcggc attgcaatat atcgcacctt actccggttg tacgatgggt gaattcttcc
     8521 gcgatcgtgg cgaagatgcc ttgattgttt atgacgattt gtccaaacag gctgtggctt
     8581 accgtcaaat ttccctgctt ttgcgccgtc cgcccggccg cgaagcctat cccggcgatg
     8641 tgttctacct gcactcccgt ctgttggaac gtgcggcacg tgtcaatgaa cacgaagtgg
     8701 agaaattgac caacggcgaa gtaaaaggca aaaccggttc tctgaccgcg ttgccgatta
     8761 tcgaaaccca agcgggcgac gtatctgctt tcgttccgac taacgtcatt tcgattaccg
     8821 acggtcagat tttcttggaa accgacctct tcaacgccgg tatccgtcct gcaatcaatg
     8881 ccggtatttc cgtatcccgc gtaggcggtg ctgcacaaac caaagtgatt aaaaagctgg
     8941 gtggcggtat ccgtttggcg ttggcacaat atcgtgaatt ggcggcgttc tcgcaatttg
     9001 catccgattt ggatgaagct acgcgcaaac aattggagca tggcgaagtc gtaaccgaac
     9061 tgatgaaaca gaaacagttc agtacgttga atacggctga aatggctttg accctttggg
     9121 caatcaacaa cggttcgtat tctgatgttc cggttgccaa agctctggct tttgaatctg
     9181 aatttttgag ctttgtccgt acccaacatc cggaagtttt ggaagccgtc aatgcttcag
     9241 gtgcaatgtc cgacgagagc gagaaaacgc ttgaagcagc catgaaatcc ttcaaatctt
     9301 cttacgccta tcaggcataa ggcttgaagt gaaaggagtc tgaaatggca gtaggaaaag
     9361 agattctcac caaaatccgc agtgttcaga atacccaaaa gatcactaaa gcgatgcaga
     9421 tggtgtcaac ctctaaaatg cggaagactc aggaacggat gcgtttggcg cgtccgtatg
     9481 ccgaaaaagt gcgtatggtg atgagccatc ttgcgcaaac caataccgat catggtattc
     9541 cgttactgga atctcatcgg gaaatcagac gtgtcggttt tattttgatt acgtctgata
     9601 agggtttgtg tggcggtttg aacgccaacg tgctgaaaaa gtttttggca caagttcaag
     9661 agtatcggaa tcaaggtatt gaagaggaaa tcgtatgctt tggcagtaaa ggtctgatgg
     9721 cgtgtcagag cattggtctg aatgtggttg ccagtgccgt aaatttgggc gataccccaa
     9781 aaatggaaat gctgctcgga cctttgacag aactcttcca acggtatgag aaacatgaaa
     9841 ttgacagaat ccatctggtg tattcgggtt ttgtcaatac catgcgtcaa gaaccgagaa
     9901 tggaagtatt gctgcctatc ggtgagaacg tgattggcga ttcagctccc aaatcaccgt
     9961 tcagctggga ataccgctac gaaccgactg cacttgcagt gttggaatat ctggttcgcc
    10021 gctatttaga gtctgtggtt tatcaggcgt tgagcgacaa tatggcatcc gaacaggcag
    10081 cccgcatggt tgccatgaaa gctgcaacag acaatgcagg caatgccatc aaagagttgc
    10141 gtttggtata taacaaatcg cgtcaagctg cgattaccac ggaattgtca gaaattgtag
    10201 caggtgcggc ggctgtctga tgctgtctga atcctgtacg gaaattagga tacgataatg
    10261 agccaaggca aaatcgtaca aattatcggt gcggttgttg acgtggaatt tccacgcgac
    10321 atgattccgc gcgtttacga cgctttgaaa ttagacgaaa acggtctgac tttggaagtc
    10381 caacagcttt tgggcgacgg cgtagtccgt gccattgcga tgggcagctc ggacggtttg
    10441 aaacgcggca tgactgtgag caataccggt gcgcccatta ctgtgccggt aggtaaaggt
    10501 acgttgggac gcattgtcga tgtattggga acgcctgttg acgaagcagg tccgatcgat
    10561 accgacaaga gccgtgccat ccaccaagct gcccctaagt ttgacgaact gtcttccaca
    10621 accgaattgc tcgaaacggg cattaaagtg attgacttgc tgtgtccgtt tgccaaaggc
    10681 ggtaaagtag gtctgttcgg cggtgccggt gtgggtaaaa ccgtgaacat gatggaattg
    10741 atcaacaaca tcgccaaagc gcacagcggc ttgtccgtgt tcgcaggcgt gggtgagcgt
    10801 acccgcgaag gtaacgactt ctaccacgag atgaaagatt ccaacgtatt ggataaagtg
    10861 gcaatggttt acggtcagat gaacgaacct ccgggcaacc gtttgcgcgt cgcattgacc
    10921 ggtttgacca tggcggaata cttccgtgac gaaaaagacg aaaacggcaa aggccgcgac
    10981 gtattgttct tcgtggacaa catctaccgt tacaccctgg ctggtaccga agtatccgca
    11041 ttgttgggtc gtatgccgtc tgcagtgggc taccaaccga cattggcaga agaaatgggt
    11101 cgtttgcagg agcgtattac ctctacccaa accggttcta ttacttccat ccaagccgta
    11161 tatgtacctg cggacgactt gaccgacccg tctccggcaa caacgttcgc ccacttggac
    11221 gcaaccgtcg tattgagccg cgatattgcc tctttgggta tttacccggc agtcgatccg
    11281 cttgattcta cttcgcgcca actggatccg atggtattgg gtcaagagca ctacgacgtg
    11341 gcgcgcggtg tgcagtccac cctgcaaaaa tacaaagaat tgcgcgacat catcgccatc
    11401 ttgggtatgg acgaattgtc tgatgaagac aaactgaccg taatgcgtgc gcgtaaaatc
    11461 caacgcttcc tgtcccaacc gttccacgtt gccgaagtgt ttacaggttc tccgggtaaa
    11521 tatgtcgccc tgcgcgatac cattgccggc ttcaaagcca tcttgaacgg cgaatacgat
    11581 catctgcccg agcaggcatt ctatatggtc ggcagcattg aagaagcagt agagaaagcg
    11641 aaaaccttaa actaaggagg tcggcatgag catcatgcaa gttgaggtgg taagtagcga
    11701 gcagaaaatc tattcaggcg aggcaacatt tatcgttgtt ccgactgtac agggcgaact
    11761 cggtatttat ccgcgacacg agccgattat gagtttggtg cggccggggg ctttgcgttt
    11821 gaccgttccg ggcgaggata aagaggtttt ggttgctgtt tccggcggtg ttttggaagt
    11881 acagcctgat aaagtaactg tcttggcgga tgttgccgtc cgcagtgcgg agatggatcg
    11941 ggcacgtgcg gaagaggcga aaaaagccgc agaagcgggc atttcccaag ctaaagacga
    12001 taaggctttg gcggaagcgc ataaagtatt ggctgctgcg attgcacagc tcaaaacttt
    12061 ggactatatc cgttcgcaca agaaataaac ttgtttggtt tggaaaagca cgaccgtttg
    12121 gtcgtgcttt ttattttccg gatttaaatt aaagactgtg tttggatatg gcgttggcag
    12181 gcatagcctg ctttaaggta ttcaataggg cagcctgccg cctaaccgaa a
//
LOCUS       DAWGRZ010000044        11797 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000044 DAWGRZ010000000
VERSION     DAWGRZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.044"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..801
                     /locus_tag="WHT44_000876"
     CDS             <1..801
                     /locus_tag="WHT44_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ2533083.1"
                     /translation="RTPNIKYIYQDNQGLSEARNTGIKNSNGKYIVFIDSDDFINCQI
                     LLDFLSKDDTDMPDVVFLNAVKYDKGSVSYFGEDYQPEKILNQSKVEVLKGLCRFRKF
                     PGSAWNKIIKRELIIREKLFFERGIYSEDIEWSMRLFNAATTFSYLDGCYYYYRQGRK
                     DSITGTVSEKSIKSLLYILEKNAEMEFNRDISSYLYSFLSYEYLVLLFIMTSKNIECD
                     ADIKRRAYHLRFMLLKSNKLIYKLIFPIITLLGVDITGRILKAIRGNI"
     gene            842..1372
                     /locus_tag="WHT44_000877"
     CDS             842..1372
                     /locus_tag="WHT44_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ2533084.1"
                     /translation="MNAIRTFQNRTPEIHETCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLAGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(1406..1522)
                     /locus_tag="WHT44_000878"
     CDS             complement(1406..1522)
                     /locus_tag="WHT44_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HEZ2533085.1"
                     /translation="MPSEPEKRLSDGIFACNSNADGQNQGASDTVIGCVPSA"
     gene            complement(1541..1728)
                     /locus_tag="WHT44_000879"
                     /pseudo
     CDS             complement(1541..1728)
                     /locus_tag="WHT44_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor"
     gene            complement(1747..3342)
                     /locus_tag="WHT44_000880"
     CDS             complement(1747..3342)
                     /locus_tag="WHT44_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ2533086.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMEIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LKIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3449..3844)
                     /gene="hisI"
                     /locus_tag="WHT44_000881"
     CDS             complement(3449..3844)
                     /gene="hisI"
                     /locus_tag="WHT44_000881"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ2533087.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRILMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRALRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(3875..4642)
                     /gene="hisF"
                     /locus_tag="WHT44_000882"
     CDS             complement(3875..4642)
                     /gene="hisF"
                     /locus_tag="WHT44_000882"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ2533088.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAANPENTRWEIFTHGGRNPTGLDAVEW
                     AVEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(4655..5392)
                     /gene="hisA"
                     /locus_tag="WHT44_000883"
     CDS             complement(4655..5392)
                     /gene="hisA"
                     /locus_tag="WHT44_000883"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ2533089.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQTVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5425..6063)
                     /gene="hisH"
                     /locus_tag="WHT44_000884"
     CDS             complement(5425..6063)
                     /gene="hisH"
                     /locus_tag="WHT44_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ2533090.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPDRVSR
                     ADKVIFPGQGAMPDCMAALKRDGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(6194..7696)
                     /gene="pta"
                     /locus_tag="WHT44_000885"
     CDS             complement(6194..7696)
                     /gene="pta"
                     /locus_tag="WHT44_000885"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ2533091.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRVNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRNANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7881..8939)
                     /gene="fbpC"
                     /locus_tag="WHT44_000886"
     CDS             complement(7881..8939)
                     /gene="fbpC"
                     /locus_tag="WHT44_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ2533092.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8960..10477)
                     /gene="fbpB"
                     /locus_tag="WHT44_000887"
     CDS             complement(8960..10477)
                     /gene="fbpB"
                     /locus_tag="WHT44_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ2533093.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPSF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10545..11540)
                     /gene="fbpA"
                     /locus_tag="WHT44_000888"
     CDS             complement(10545..11540)
                     /gene="fbpA"
                     /locus_tag="WHT44_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ2533094.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
BASE COUNT         2518 a         2786 c         3585 g         2908 t
ORIGIN      
        1 cggacaccaa atatcaaata tatatatcag gataaccaag ggttgtcgga ggcgagaaat
       61 accggaataa aaaattcaaa cggaaaatat atagtcttta ttgattcgga tgattttatt
      121 aactgtcaga ttttgctgga ttttcttagt aaagatgata ctgatatgcc ggatgtggtg
      181 tttttaaatg cggttaaata tgataaggga agtgtttcat attttggcga agattatcag
      241 cctgaaaaaa tactcaatca atccaaagtc gaagttttga aaggattatg ccgatttaga
      301 aaatttccgg gttcggcgtg gaataagatt ataaaaagag aattgattat tagagaaaaa
      361 ctgttttttg aaaggggaat ttattctgaa gatatcgaat ggtcaatgag gttatttaat
      421 gcggcaacaa ctttttctta tttggacggt tgttattact attatcggca gggaagaaaa
      481 gattctatta cgggaactgt ttcggaaaaa agtataaagt cattattata tattttggag
      541 aaaaatgcgg aaatggaatt taatagggat atatcgagtt atctttattc ttttctttcc
      601 tacgaatatc tcgttttgct ttttataatg acgagtaaaa atatagagtg tgatgctgat
      661 ataaaaagaa gggcgtatca tttaaggttt atgctgttaa agtccaataa attgatatat
      721 aagctgatat tcccgataat cacattactc ggggtcgata ttacaggcag gattttaaaa
      781 gcaatcaggg ggaatattta ataaatcctt taacaatata taccttaccg aaggaggaaa
      841 aatgaacgca atccgaactt tccaaaaccg cacgcccgaa atccacgaaa cctgtatgat
      901 agacgaagcc tgcgtcgtca ttggcgaagt gtcgcttgcc gaagatgttt ccgtgtggcc
      961 gtgcgccgtg ttgcgcggcg atgtgaacag catcaccgtc ggcgcgcgca gcaatataca
     1021 ggacggcagc gtcttgcacg tttcccacaa aaccgccgcc aaacccgaag gatcgccgct
     1081 ggttatcggc gaagacgtta ccgtggggca caaagtgatg ctgcacggct gccgtatcgg
     1141 caaccgcgtc ctggtcggca tggggacgac ggttctggac gatgccgtga ttgaggacga
     1201 agtgatgatc ggcgcgggca gcctcgttcc gccgcgcaaa cgcttggcgg gcggctatct
     1261 ttatgtcggt tcgccggtca gacaggtgcg cgtgctgacc gatgaggaaa aagccttttt
     1321 gaaatattcc gccgcgcatt atgtgaagct gtcgaaacag tacgggatgt gaaatcacat
     1381 cggcgttctt gcgtcagccc caaattcatg cggatgggac gcatccgata acggtatccg
     1441 atgcgccttg attttgaccg tctgcgtttg aattgcaggc aaaaatgccg tctgaaagcc
     1501 ttttttcggg ttcagacggc attttattgc cgattgtttt ttaaagtttg accgaatgtt
     1561 cgcgcgtttc gtggaacacg atgtccggcc agcgttcttg cgtcagccct aaattcatga
     1621 ggacgggatg cccgataacg gtatccgatg cgtcttgatt ttgatcggtg catttgagtt
     1681 gcaggcaaaa atgccgtctg aaagcctttt ttcgggttca gacggcattt tatcgccgat
     1741 tgctttttac agtttgaccg aatgttcgcg tgtttcgtgg aacacgatgt ccggccaacg
     1801 ttcttgcgtg agtcccaaat tcacgcggtt gggggcgagg taggcgaggt tgccgcctgc
     1861 gtcgatggcg aggttgcccg cgttggcttt ttcaaattca gccagttttt tcttgtcgtc
     1921 gcacgatacc cagcgcgccg accagatgga tgcgctgtcg aacacggctt ctacgccgta
     1981 ttcgttggcg aggcgcgagg taacgacttc aaactgcaac acgccgaccg cgcccaaaat
     2041 caaatccgcg ccgctcatcg gtttgaacac ctgcaccgcg ccttcttcgc cgagctgttg
     2101 caagcctttt tgcagttgtt tgattttcag cgggtttttg atgcgtacgc tgcggaacag
     2161 ttcgggtgcg aagaatggga tgccggtgaa cgccagttgt tcgccttcgg agaagctgtc
     2221 gccgatttgg atgttgccgt ggttcgggat gccgataatg tcgccggcgt aggcttcttc
     2281 aaccagctcg cggtcgtgcg acatgaaggt aaccacgctg gaggcggcga tttcgcggtt
     2341 gatacgcagg tgtttcatct tcatgccgcg ctcgaatttg ccggagcaga cgcgcaagaa
     2401 ggcaatacgg tcgcggtgtt tcgggtccat attggcttgg attttgaaga taaatccgga
     2461 aaacttcggc tcgtccggct cgaccatacg tacggtcgcg tcgcgcggtt tcggcgcggg
     2521 cgcccagtca atcaatgaat tgaggatttc ctgaataccg aagttgttaa tcgcagagcc
     2581 gaagaatacg ggcgtgagtt cgccggcgag gaattcgtcg agattaaact cgttggaagc
     2641 cgcctgcacc aattcgattt cgtcgcgcaa ctgctggatt tccaacggaa agcgttgttc
     2701 caattcagga ttatcgatgc ctttgatgat gtcgaactcg tgcggcaggc gttcgccgcc
     2761 agcttcaaag agataaattt catcgttcag gatgtggtac acgcccttga agtttttgcc
     2821 cataccgatc ggccaggtaa cgggcgcgca gcggattttt aaaatgtttt ccacttcgtc
     2881 caaaagttcc agggaatcgc gcacttcgcg gtcgtatttg ttcataaacg taacaatcgg
     2941 tgtatcgcgc aggcggcaga cgtttaagag cttgatggtt tgcgcttcca cgccttttgc
     3001 cgcgtcgatg accattaatg cgctgtccac ggcggttaaa acgcggtagg tgtcttcgga
     3061 gaagtcttgg tgtcccggcg tgtccaagag gttgacggtg tggtctttgt aatcgaactg
     3121 catcacactt gatgccacgg aaatgccgcg ctgcttctcg atttccatcc agtcggaagt
     3181 ggcgaatttg ccggttttct tgccttttac cgtacccgcg ctctgaatcg cgcccgaaaa
     3241 cagcaagagt ttttcagtca acgtggtttt acctgcgtca gggtgggaga tgatggcaaa
     3301 cgtgcggcgg cggcgcactt ggtcgaggat ttcttgggac atggttttct ttgcaaaaag
     3361 gttcaggccg cttttcagac ggcccggaca gtgtttgaga cggcgaaatt gtacaaaaaa
     3421 atgcctgata attcaatgtt ggaggcggtc agtgcgtgct gccgtaaatc tctttttcgt
     3481 ctttcaggac ggcatcggcg gtttcccacg cgctgccacg ccagactttg taaaagcagc
     3541 tttctcgccc ggtgtggcag gcgatgccgc cgttttgggc gatgagcatc acaatggcgt
     3601 cgccgtcgca gtcgaggcgc agtgcgcgga ctttttgcgt gtgtcccgac tcttcgccct
     3661 tcatccattg tttttggcgc gaacggctgt aatagtgggc aaagccggtt tcgacggttt
     3721 tttgcagggc ttcggcgttc atccacgcca ccattaaaat acgtttggtt tcggcatctt
     3781 gggcgatggc gcaaaccaaa cctttttcgt caaatttgac ggcttcaagc aggtttttat
     3841 ccatatttcc tttcagacgg catagtcgag gcggtcagag gcgcacttcg atgccggctt
     3901 cgcgcatagc gcgtttggct tcgcggatgg cgatttcccc gaaatggaaa atgccggcgg
     3961 caagtacggc atcggctttg ccttcggtta tgccttcaat caggtgccgg acattgccga
     4021 ccccgccgga ggcgatgacg gggatgtcga cggcttcggc aacggcgcgg gtcagcggca
     4081 ggttgaaacc ctgtttcgta ccgtccctgt ccataccggt gagcaggatt tcgcccgcgc
     4141 cgcgtttttg catttcgacc gcccattcca ccgcatccaa accggtcgga tttcgcccgc
     4201 cgtgggtaaa gatttcccag cgtgtgtttt cggggttggc ggctttggca tcgacggcgg
     4261 cgacgatggc ttgcgaaccg aaaaatccgg cggcttcgtc aattaaatcg ggacgggtaa
     4321 cggcggcggt gttgatgctg actttgtccg cgccggcatt gagcaggcgg cggatgtcgg
     4381 caacggtgcg tacgccgccg ccgacggtca gggggatgaa gacttgtccg gcaacctctt
     4441 cgatgatgtg caggatggtg tcgcggttgt cggatgaggc ggtgatgtcg aggaaggtca
     4501 attcgtccgc gccttcgccg ttgtagcgtt tggcggcttc gacggggtcg cccgcgtcgc
     4561 gcaaaccgat gaagttcacg cctttgacga cgcgcccgtc ttttacgtcg agacagggga
     4621 tgatgcgttt tgccagtgcc ataatcggat gcctttagtc gagggaatct gccagttgct
     4681 gcgcttgggc aaaatcgatg ctaccctcgt aaatcgcgcg gccggtaatc gcgcctgcta
     4741 cgccatgttt ttcggcggca cacagggcgc ggatgtcgtc caagccggtc agtccgccgg
     4801 aggcgatgac gggaatgcgg acggtttggg cgagtttgac cgtcgcgtcg atgttcacgc
     4861 cgctcatcat accgtcgcgc ccgatgtcgg tgtagatgat gctgttgacg ccgtcgtctt
     4921 caaagcgttt tgccaaatca attacatgat gcccggttac ggttgcccag ccgtcgatgg
     4981 cggccatacc gtctttggca tccagcccga caataatcct gccggggaag gctttgcacg
     5041 cctcgcgcac gaagtcgggg tttttgaccg ccgccgtgcc gataatcacg tcgtttaagc
     5101 ccaaatccaa atattgtccg atggttttca aatcgcgtat gccgccgccg agctgtacgg
     5161 ggatgtcttt ggcgacagcg gcaaggatgt ctttgatggc gggcaggttt tgcggaacgc
     5221 cggcaaacgc gccgttcaaa tctaccagat gcaggcggcg cgcgccttgt ttgaaccagt
     5281 gcagcgcggt ttcggcgggc gaatcggaaa agacggtcgc ctcttccatc agcccttgtt
     5341 tcaggcggac gcagcgtcct tctttcaaat cgatggcggg tatcagcagc ataatttttc
     5401 tccttgtgcg gggccgtgtc cggcttacca gtttaaaaag tttttcaaca tcgtcagccc
     5461 ggcatcgtgg cttttttcgg tgtgaaattg cgtggcgaat acgttgtctt tgccgacgat
     5521 gcaggcaaac ggggacgggt agtcgctttc gcccaatatg gtttcgggat tttcgggggc
     5581 gaaatagtag ctgtggacga agtaaaaacg cgtgtcttgg ggaatatctt taaacagcgg
     5641 gtggttttgg gtttggcgca cggtgttcca gcccatatgc gggactttca gacggcatcc
     5701 ctgcgggtcg cggaggtcgc gctcaaagcg tctgactttg ccgccgaacc agcccaagcc
     5761 gtcggtgttt ccttcttcac tgtggtcgaa taaaagttgc gcgccgacgc agattccgaa
     5821 aaacggtttg ttttttaagg catctttgac tgcctcgtcc aaaccgtctc gttttaatgc
     5881 cgccatacag tcgggcatcg cgccctgacc gggaaaaatg actttgtcgg cgcgggacac
     5941 gcggtcgggg tcgccgctta aaaagatttc ggtatttttt ccggcaagct gccccgccgt
     6001 ccggacggat ttcaatacgg aatgcaggtt gcccataccg taatcgataa tggcggtttg
     6061 catggcttcc tcctcttttt ttgcaatatg gctgcgattt taacaaacaa atgtgccgtg
     6121 ctgataaaaa tgccgtctga aaacgggagt ctgtcttcag acggcatagg gtttaaaccc
     6181 ggaaagccgt ttgtcagcct tccatttgtt ttgcctgaac ggcagtcagg gcgatggtaa
     6241 acacgatatc ttctaccagt gcgccgcggg agaggtcgtt gaccggttta cgcaggcctt
     6301 gcagcagcgg gccgacgctt aagacgttgg cgttgcgttg gacggcttta taggtgcagt
     6361 tgccggtgtt caggtcgggg aagaccaaaa cggttgcctg tcctgccacc gggctgcccg
     6421 gagctttgga tttgcccaca cccggcacgg ttgccgcatc atattgcagc gggccgtcga
     6481 tggcgaggtc ggggcgtttt tcccgggcaa gtttggttgc ttcgatgacg gtatcgacat
     6541 cggggccgct gccggagttg acggtggagt aggaaatcat cgccactttc gggtcgatgc
     6601 cgaaggcttt tgcggaatcg gcagactgga tggcgatgtc ggcaagctgt tgcgcggtcg
     6661 ggttcggatt aaccgcgcag tcgccgaaga cgaggacttg gttgggcagc agcataaaga
     6721 atacgctgga cacgaggctt gcgcccggtg cggttttaat cagttgcaaa gcggggcgga
     6781 tggtgttggc ggtggtgtga accgcaccgg ataccaaacc gtccacatca ttttgcgcca
     6841 tcatcatcgt accgagtacc acggtgtctt gcagttgctt gcgcgcgtct tcgggtgtca
     6901 ggcctttgga tttgcgcagt tcgcacatcg gctcgacgta ttgttcgacc aatgaggcgg
     6961 gatcgatgat ttccaaagag tcgggcaggc tgatgccgcg ttctttggca acggcttcga
     7021 cttcttcgcg tttggcaagc aggacgcagc gggcaatgcc tttttcgtgg cagatggcgg
     7081 cggcttggac ggtgcggggt tctgcgccct caggcaggac gatgcgtttg tcggcttggc
     7141 gggcgaagtc gatcaggttg tagcggaatt gcgccggcga caggcgtttt gcttcgcggc
     7201 ctgccaatac ggatacgtct ttcagcgcgt cgctcgaacc gaagaaggtc aggccggttt
     7261 tttcggctgc cgcttcggca acggaggctg ccgcgccgtc cacgacaaaa ccttccaata
     7321 cgcccggcgc ggcggcgaag aactgtttgg caaggttcac ccgatttgcc agttcgtcgg
     7381 catcggcgtt gtcggaacgg acggcgaaga cggctgccgc gtcaagggac aatgccagtt
     7441 cgacgttttt gcctgcgagg tagattttgt cggcatcggg cgcgatgcct tcgatgacga
     7501 ggttggcggc atcgagtgcg gcaactttgc cgaccagtgc gtcgaaccag tcgtcgcttt
     7561 tgccttgcgc gagcagggtt tcggcggttg cgtcaacggc ttggaaaatt tgtgcgtcca
     7621 gtgcttttgc aaaggcttgt gcggcggcgg aggcgtccag tccggcagat acgggtacga
     7681 tgagtacttt tgccatgata tatcctttcg tatgctgcgg tgtgcggcat atgtggttgg
     7741 aaggggcggc atataggcag aaacggctgc ctgcgtgccg tgcgtgccgt gtttggcttg
     7801 aggcgcgcag gttgaatata gcaaacaaat tctgtttcca acaagataaa tatccgcagg
     7861 cttgtggatg ctgccgcctt tcagagggta tttccgggga agaacagggc gggaccgtcc
     7921 aaatggagga cggcggaaat gccgtctgac agggtggggg cggaagggag gttgagcgtg
     7981 aggacggttt gtccgacccg gaggctgatt tcggtatgcc gcgctttggg cgtggttttg
     8041 agaaccacgg cgtgaatgga ggcggtgggt gcggaatggg ggtgaaggct gaactgttcc
     8101 ggacggatga gcagtgtgcc gcgtgtgcct gcgggtgcgc cgctttgaac gggcaggcgg
     8161 cccaatccgc aatcggcggt gccgtcggcg ttgagcgcgg cggggaacac gatgccttcg
     8221 ccgataaaca gggcggcatc aaggtcggca ggttgtcggt acaattcgtg agggcttgca
     8281 gtttggagga tgcgcccctg tttcatcacg gcaatccggt cggcgtattg cagggcttct
     8341 tcgcggtcgt ggctgacgaa aacggcagat ttgccgttgg cgcgcagggc ggcaatcatg
     8401 tcttcgcgaa tctggcggcg caactgttcg tccagcgcgc tgaagggttc gtccaacaaa
     8461 atcagttcgg gatcgggtgc gagggcgcgg gcgagggcga cgcgctgttg ctgtccgccc
     8521 gaaagttcgt gcggatagcg tccggcaagt tcggaaatgc cggtcaattc caacatagct
     8581 tcgatgcgct gccgctcttg cgccgtcttg cctttgccgt tgcccagccc gtaggcggtg
     8641 ttgcggtaaa cggtcaggtg ggggaacagc acaccttcct gtacgacata acccaaacgg
     8701 cgttcgcgga cggggaggtt ggtatttttc gagaagatgg ttctgccgga aagcgaaatt
     8761 tcgccaaaat cgggttgttc aaaaccggca aggcagcgta aaagggtggt tttgccgcag
     8821 ccggacgcgc cgacgataaa gaggatttcg cccgggtcga ggctgagcga aatgtcgttt
     8881 aaaactgggg tgttttgaaa acttttggac aggtgtccga tgtgcagggc ggcggtcatg
     8941 gcggtacttc ctcaagctgt tatttgaagg cgtatttctt cagcaggaat acggggatgc
     9001 cggaaaataa taccagcatc agcgcgtaag gggtggcggc ggcgtattgt gcgtccgatg
     9061 tgtattccca aacggcggtg gagagtgtgt ggacatcgtc ggtggtcagc agcagggtgg
     9121 cggtcagctc tttcatcagt ttgaggaaga cgagtgcgaa tgcggcggta atgccgggca
     9181 ggatggacgg cagtaccaac gtcctgaaaa taaagaagtg tccgcgcccc aatgttgcgc
     9241 cgacctgttc catccctttt gggagttgtt ccaaggaagt cctcagggtg gtttgcgcca
     9301 tcggcaggta aagcatgaaa taggcaagga tgacgacgat aaaggtttgg taaacggcag
     9361 gggtgtagtt gatgctgaaa taaaccaagg atagggcgat aaccaaaccg gggacggcgt
     9421 gcagtaaaaa cggcagcctg tctatccaaa cggttaaaaa attgcgatag cgaaccgatg
     9481 cccaaacaag gggcaaggca cataatatag tcaaaatcgc acctaaagcc gatacgctta
     9541 aggaacggat aaaggcatca aatacggata cgagcgcgaa tgtgccggaa gtgccgacca
     9601 tcatccaatg tatcaatacg ccaaagggga taataatgcc caaagtcaac aagctgctta
     9661 aaaaaacaat cgcgccaatc tgaccgggca gtttgagggt tttgacggga taaggacggg
     9721 caacgccttt gccgctgtgg taaatcttgg ctttgccgcg aaatatgctt tctccaaata
     9781 cgacgatgcc gcacaccgcc attaaaacag cggaaagcag ggcggcggta ttgttgttgt
     9841 aggacatttc gtattcttgg aaaatggcgg tggtaaaagt ggggtagttc aaaatggata
     9901 ccgcgccaaa ttcgaccagc atatgcaggg caatcagtaa cacgctgctg ccgatggcgg
     9961 gtttgagctg ggggaggatg gcggaaaaaa aggtttgcag gcggcttttg cccaaggaca
    10021 ggctgacttc ttcgtaagac aggctgatgc gtttgagtgc cgcctcgacg ggcaggtagg
    10081 cgagcgggaa cgaggacagg ctcataatca tcactgtccc ccaaaagcct tcgacacgga
    10141 aggtcaggct gatccaggtg aaacagctga caaatgaggg gatgcacaaa ggcagggtga
    10201 ttgccgtctg aaaaaaggtt ttgccgaaga agcggtaacg ttggaacaaa agggcgcagg
    10261 caatgcccaa aacaatggaa atcagggtaa cgcccgccat catcgtcaag gtgttggaga
    10321 gcaaatccca catacgcggg cggaacaaca gttcgacggc gcggttgatg ccgacctgcc
    10381 acgaacgcat agcgacatat aaaaaaggca gggtaagcgg tagggcaatc agtaggatga
    10441 ggccggtaag ccaaatgggt atttttttag gagacatagt gttttttatc ggcaaaacgg
    10501 gcggacagta taaatgtcca cccgtttgac aatccgaaaa cggcttattt cataccggct
    10561 tgctcaagca gccgggtggc gtgttctttt tcggaaacag tggtggcgga cacttggggt
    10621 gcttccaact tggcgatggg ttccaaattg aaagtggata ccacgtgcgg attcaaagga
    10681 tattcggcac ggacggcggt cagggcgcgc tgtccttcct tgctggcgag gaaggcgacg
    10741 aattttttcg cctcatcctt gttttgggag gattttaaca cggctgcgcc ggaataggta
    10801 acgagtgcgc cgggatctct gtggcggacg aaattcaggc gggtgtggac attttgtacg
    10861 cctttttcac gcgcaaaagc gtgccagtag tagttgttga tgagggcggc atcgatttcg
    10921 ccgttttcaa ccgcttgaag ggcgacggag tttttagcgt aaggcttgcc gtattctttc
    10981 agacctttga gccatttcaa tgcggccgct tcgcctttca gtttgacgat ggcgacaacc
    11041 tgttccaaga acgcgccgga agtgggggcg taaccgatgc ggtttttcca tttcggcgtg
    11101 gcgtaattca ggacggattt ttccaaatct ttttcagaca gtttgcgggt gtcgtaaacg
    11161 acgacgcgcg aacgtccgct cagtgccacc cagtcttttt tggcggcaac cggcacgccc
    11221 ttgccgcgtg tttcgttgat ggtggaggcg ggcaggggct ctaggaggtt ggcggcggaa
    11281 agggtggcga gtgccgggat ttgttcggaa tagaatacgt cggcggggct tcggctgcct
    11341 tcttctttga tttgaccggc aagctggtcg cctttggcgc tgttgagttt gactttgatg
    11401 ccggtagccc gggtaaaggc atctgcaacg gcttgtgctg cttctttgtg ttggccgttg
    11461 tacacggtaa tgtctgccag cgcgggggtt gcggcggtca gggctgcggc aagcagtgcg
    11521 tatcggatag atgttttcat atcgattttc tcctaatgaa tgagagtgta taccttgtta
    11581 agacataacg gtgtgtagtg tattccttct tttttataaa tgcaaataat tattttttaa
    11641 atttgttgtt gtccgatccg gttattgttt gttctgactt gtattttttc cgtgagtctc
    11701 gcccgtaagg cggaagtggc gggcaatgcg tggcggaatg tgggtaaagg cggcattttg
    11761 atttgtcgga atgcttgaga acccctctct ttaaaac
//
LOCUS       DAWGRZ010000045        11637 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000045 DAWGRZ010000000
VERSION     DAWGRZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11637
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.045"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(339..761)
                     /locus_tag="WHT44_000889"
     CDS             complement(339..761)
                     /locus_tag="WHT44_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ2533095.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGKAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(879..1550)
                     /locus_tag="WHT44_000890"
     CDS             complement(879..1550)
                     /locus_tag="WHT44_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ2533096.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDAVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            complement(1566..2192)
                     /locus_tag="WHT44_000891"
     CDS             complement(1566..2192)
                     /locus_tag="WHT44_000891"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ2533097.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIHNVRIAAVLSNSETAAGLQWA
                     AERGIPTDSLNHKNFTSRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCARYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGVVPILDGDTA
                     DDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2299..3117)
                     /locus_tag="WHT44_000892"
     CDS             complement(2299..3117)
                     /locus_tag="WHT44_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ2533098.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3376..3816)
                     /locus_tag="WHT44_000893"
     CDS             complement(3376..3816)
                     /locus_tag="WHT44_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ2533099.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSEPSVLLACGGNLPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEELADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3879..5285)
                     /locus_tag="WHT44_000894"
     CDS             complement(3879..5285)
                     /locus_tag="WHT44_000894"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ2533100.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     DFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAVVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEDYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5455..6570
                     /gene="lptF"
                     /locus_tag="WHT44_000895"
     CDS             5455..6570
                     /gene="lptF"
                     /locus_tag="WHT44_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ2533101.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6567..7637
                     /gene="lptG"
                     /locus_tag="WHT44_000896"
     CDS             6567..7637
                     /gene="lptG"
                     /locus_tag="WHT44_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ2533102.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELIPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7847..10432
                     /gene="acnB"
                     /locus_tag="WHT44_000897"
     CDS             7847..10432
                     /gene="acnB"
                     /locus_tag="WHT44_000897"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ2533103.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLASIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10574..11569
                     /locus_tag="WHT44_000898"
     CDS             10574..11569
                     /locus_tag="WHT44_000898"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ2533104.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEIFESPASIVFDQAENRMHTIKAVMVAALGD"
BASE COUNT         2676 a         3234 c         3026 g         2701 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactataatt caataaatta atatatggct taaaataacg
       61 ggattctcgc ctcccgcccg cccgcagaag caggcggata tcattttaaa acgcggcatt
      121 taaaatttga ccgaaaattg ttgacaatcc ggaatcaagt ctgcacaata ccccgacaag
      181 tccaagtatt ataaaggctg aataaagagg aaacagcagg cagatatatt cgggaggtgc
      241 agtccgaata tatctgcttt tttatgcgcc tccggattgc ctgccgcacc tttcccttca
      301 gacggtatca gccgtttccc cataatgccg cccgatgcct atttatctgc cccggcaatt
      361 tcaaaactgt gggtaatctt tgccgctttg cccaacataa tcgaagccga acagtatttt
      421 tcggcagaca tctgaacggc gcgctcaatg gccgattctt tcaaatcatg cccgaatact
      481 ttgaaatgga tgtggatttc ggtaaacacg cgcggcgcat cgtccgcccg tttcgccgta
      541 accgtcgcac ggcagtcagt cactttctga cgctgttttt cggcaatcat caccacatcg
      601 atgctcgaac agcccgccac gcccaacagc agcatttcca aagggctggg cccgcgctta
      661 gccttacctt ctgccgccga cccctccata acgacgctgt gcccgccttc cgtcgtgccg
      721 acaaaacaca tcccgtctat ccattttgat gtaacctgca tggtgtcatt cctgaaaata
      781 gcgttaaaac cgctttgcat atggcgttat tgtaaacaat ttcaagcggc ttatgcagaa
      841 atatggacaa aacggcaaaa aaacacttga aaaccgattt acggtttggc tgcctggccg
      901 ttgatctgca ccgatttgag tttcagcgta taggttttgc cgtcgtcggt atagccgatt
      961 tgtgccggaa tattgttcag ggacggtgcg aagaaataca ttaccgcatc gtcgccgcgc
     1021 cgcacccgat atttgacgac ttcggtttcc acgccgccta tgctgtattt tcctgtaccc
     1081 gccttattca aaccgccgac ggaataaagt tttttgccgt tggtgatttt cagccccggg
     1141 gggagtttcg cgtcatttgc cgccaactgc caggcaagcg tgaacaaatc catagccttg
     1201 gggctttgct cggttttgct ctcgcccgct ttgccgtaag ttacgctgcc gtcggcgaat
     1261 ttggcttccg catacagttt gcccctgcgt atgtctctat agtaggtagg gtgcagggta
     1321 ttgccgacaa ccgtaccgcc ggactcgaaa cggatattgt atagcggcac tttaatcgtc
     1381 gaaacgattt tgtaagcatt gccgctgcgt tcaaatgtca tcgtggcggg aatgccgtag
     1441 ctgccggaat agtgcagcac ggcggattgg ggcagccctg ccgcatacgc gcacggcagg
     1501 gcggcggaca aaatggcggc ggaaaatata tttttaaaag tcttcatcat ttgctcccgc
     1561 ccggtttacg ccgtcagaaa acgggcggca tcggcgtttt ccgaatttct gacgcggttt
     1621 ccctcaataa tcaggcggcc ggcggcaaaa tcggcaacgg ctttcggata aagtttatgc
     1681 tcgacagcca aaacccgtgc ggcaatatcg tctgccgtat cgccgtcgag tatcggcaca
     1741 accccttgcg atacaatcgg gccgcaatcc agttcggcag taacgaaatg gatggtgcag
     1801 ccggcaacgc ggcagcccgc ctccaaagcg cgttcgtgcg tatgaagtcc ggtaaacgag
     1861 ggaaggatgg acgggtgaat gttcatcagc ctgccttcgt aacgggcgca aaactcgggg
     1921 gtcagaatcc gcataaaacc tgccaaaacc accaagtcgg gttgatatgc gtcgattttc
     1981 tccatcatgg cggtatcgaa ggcaagccgg gatgtaaagt ttttatgatt caggctatcg
     2041 gtcgggatgc cgcgttcggc cgcccattgc aaaccggcag ccgtttcgct gttgctcaac
     2101 acggcggcaa tgcggacgtt gtgaatggcg gcattgacga ttgcctgcat attgctgccg
     2161 cgtccagaaa tcaggatgac gatgtttttc ataatggtgc gcttttgaaa gggatgccgt
     2221 ctgaaccgct gtttggtggt ttcagacggc atttgccgta aaaatgcccg aaaacctgtt
     2281 tcgggcatgg attcggactt aatttacttt tttgatgtcg acttgagccg gctgcttggc
     2341 gggcgcgttt tcgggtgcgc cgattttgac cagtttcaca tcaaatacca aagtggcgtt
     2401 cggaccgatt ttgtcgcccg caccctgttc gcggtaggca aggttggacg ggatgtagaa
     2461 cgtggcttcg ccgccttctt tcagaagctg tacgccttcg gtccaacccg gaatcacttg
     2521 gctcaaaggg aaggtgaccg ggccgccgtt ggctttgctg ctgtcgaata ccgtaccgtc
     2581 aatcaggcgg ccttcgtatt ccacggtaac gatgtcgtct ttggtcggct gtttgccttc
     2641 gccctgtttg gtgattttgt attgcaggcc ggaagcagtg gtcttcacgc cgtctttggc
     2701 ggcattttct ttcagaaagg cttcgccttt ttctttattg gccttcgcgt ccgccttgtg
     2761 tttttctacg gctttagcct gttgttcctg aaggaatttc atcatgactt cctgagcctg
     2821 ctcttcggtc attttgattt ctttgccgtc atacactgcc tgcatggctt cggtaaagac
     2881 tttcaaatcg atttccgcgc cctgttcctt catttgcttc agggagcgtc cgatgtccac
     2941 gcccatcgca tagcttgcct gctgcatcgt gctgccgatc gaagaggtgt cgccctgcgc
     3001 ggaagaagcg gcggcaggtt cggatgcaga tgcgggggcg gcttcttttt tgccgcaggc
     3061 ggaaagtgcc aaagcggcgg aaagggtcag tgcgctgatt ttgaaaatgg tgttcatgat
     3121 ggatcttcgc tgtcgataag gtcggaaaaa cgggattata gccgagtttg aatgtttcaa
     3181 cacacaggat gacacataaa gcgtcaatcg tgtgttgccc tgttttggaa gggattgaac
     3241 cttccaaaat aagttttgat tctaccgccc cgagggacag atgtccaagt ggcggggttc
     3301 aaccgataag gaaattttaa tcaaatagaa tcaagcctgt ttaaattttg taaatgcggc
     3361 atttcagacg gcattttatg ccttgccctc catgccgtga tgttcgatgg caaaaccgct
     3421 tcggcggtag gcggtaaagc gttcgcgcgc gtcggcgagc tcttccaagc tgttgccgac
     3481 gatttccaaa acgcgcgcgg gcaagaccgg agcggtgttc caaaaaccgt cggacaggtt
     3541 caaaacggtc atgccttcgg gaattcgggg cagattgccg ccgcaggcaa gcaggacgga
     3601 tggttcagac ggcatggctt cttccgtttc ccaaatttcg tgcggaatga aactctcggc
     3661 ctcgtattgc caaagcattt tgtccaattc ctgaagctgc ccgaacgaat cggaccacac
     3721 cagtatcctg ccgccgtccc gaatcgcacg ggcaatcagg cggcaggtga aaatcggaac
     3781 ttgggcaacg tgcgtgtaaa aggtggcttt cggcatattg tttgaacatt tggcaggata
     3841 atgccgtctg aaaggcttca gacggcattg tggcaaaatt aaagattccg cagatagttc
     3901 agcagcaagg gaacgggacg gccggtcgca cctttttccg caccggattt ccacgccgta
     3961 cccgcgatgt caaggtgtgc ccatggatag tcttcggtaa agtaggatag gaatgttgcg
     4021 gcggtaatcg tgcccgcgcc gggcgtgccg atgtttggaa tgtcggcaaa gttggatttg
     4081 agttggtctt tgtaggtctc aaagagcggc agttgccatg ctttgtcgtc cacgttgtag
     4141 gaagcggcaa gcaggctgtc gatcaaatcc tgattgttgc ccatcacgcc gctgacatcg
     4201 tgccccaagg caacaataca cgcgccggtc agggtggcga cgtcgatgac ggctttgggt
     4261 ttgaactgct cggcgtaagt gagcgcgtcg cacaaaatca gacggccttc ggcatcggtg
     4321 ttcaacactt cgatggtcag ccctttcata cttttcacga catcgcccgg tttgtttgcc
     4381 gcgccggaag gcatattttc acaagtggcg acgacggcaa tcaggttgat cggcagttgc
     4441 agtttgacgg cggcgcagaa ggtgctgatg acggttgccg cgccgcacat atcaaacttc
     4501 atttcgtcca tgttcaggcc gggtttgagg gagatgccgc cggtgtcgaa ggtaatgcct
     4561 ttgccgacca agacgacggg ggcggcttct ttgtcggctg caccgaaata gctcagttcg
     4621 accaaatagg gatcttcgac actgcctttg gcgaccgacc aaaacgaacc catgttttct
     4681 ttgatgtagt ctttttcgat gattttggcg tgcgcgccca gtttttcggc ttcggctttg
     4741 gcggtgcgcg ctaaaaattc gggcgtgcat tcgttgggcg cggcgttgcc caagtcgcgg
     4801 cagaggcttt gtccgtaaac ttgcgcttcg gcgacgcgca aggcttcttt aacgacggct
     4861 tcgtgtgcgc tgtggaacac ggcggtttca aatttggcgg gcttggcttc ttttttgtag
     4921 cggtcgaaac ggtaggcggc gttgccgaac gcaatcgcaa acgcttcggc tacggcggcg
     4981 gcttgcgctt catcgaaggc atggatgtcc acattgaccg tttcctgatt ttgcgcccat
     5041 ttggcggctt cggcggcggc cttgttcaat gcggcgcggc cggtgctttt cagacagcat
     5101 acggcaacag cctgcaaacc gttgcctgtc gggatttttg tgtcggcaaa atcttgacct
     5161 tcttcaagcg aagacaaaag ggcaagggcg gtcggatggt ttaattgcgc ctcttcggtg
     5221 cagacaaata actgtgcgcc tgcctgctgt tcctgcaaga tttcggtttt tgtgctaaat
     5281 tccacgttta ttctcctgat tgagacggtt gtcggtagtt ttcggacggc ctttcgctca
     5341 aaagaccgtc tgaagacggc tggcacgatt gtaccccatt tgaagcaccg tctgaaacct
     5401 tgcgcggaca atccgcctgc gccgaaccgc ttaccgcccc ctgaccgcga ttctatgatt
     5461 tatcaaagaa acctcatcaa agaactctct tttaccgccg tcggcatttt cgtcgtcctc
     5521 ttggcggtat tggtctccac gcaggcaatc aacctgctcg gccgtgccgc cgacgggcgt
     5581 gtcgccatcg atgccgtgtt ggcattggtc ggcttctggg tcatcggtat gacgccgctt
     5641 ttgctggtgt tgaccgcatt tatcagtacg ttgaccgtgt tgacccgcta ctggcgcgac
     5701 agcgaaatgt cggtctggct atcctgcgga ttggcattga aacaatggat acgcccggtg
     5761 atgcagtttg ccgtgccgtt tgccgttttg gttgccgtca tgcagctttg ggtgataccg
     5821 tgggcagagc tacgcagccg cgaatacgct gaaatcctga agcagaagca ggaattgtct
     5881 ttggtggagg caggcgggtt caacagtttg ggcaagcgca acggcagggt ttattttgtc
     5941 gaaaccttcg ataccgaatc cggcatcatg aaaaacctgt tcctgcgcga acaggacaaa
     6001 aacggcggcg acaacatcat cttcgccaaa gaaggtaact tctcgctgaa cgacaacaaa
     6061 cgcacgctcg aattgcgcca cggctaccgt tacagcggta cgcctggacg cgccgactac
     6121 aatcaggttt ccttccaaaa actcaacctg attatcagca ccacgccaaa actcatcgac
     6181 cccgtttccc accgccgtac cattccgacc gcccaactga ttggcagcag caacccgcaa
     6241 catcaggcgg aattgatgtg gcgcatctcg ctgaccgtca gcgtcctcct actctgcctg
     6301 cttgccgtgc cgctttccta tttcaacccg cgcagcggac atacctacaa tatcttgatt
     6361 gccatcggtt tgtttttaat ttaccaaaac gggctgaccc tgctttttga agccgtggaa
     6421 gacggcaaaa tccatttttg gctcggactg ctgcctatgc acatcatcat gttcgtcatc
     6481 gcaatcgtac ttctgcgcgt ccgcagcatg cccagccagc ccttctggca ggcggttggc
     6541 aaaagtctga cattgaaagg cggaaaatga acctgatttc acgttacatc atccgtcaaa
     6601 tggcggttat ggcggtttac gcgctccttg ccttcctcgc tttgtacagc ttttttgaaa
     6661 tcctgtacga aaccggcaac ctcggcaaag gcagttacgg catatgggaa atgctgggct
     6721 acaccgccct caaaatgccc gcccgcgcct acgaactgat tcccctcgcc gtccttatcg
     6781 gcggactggt ctccctcagc cagcttgccg ccggcagcga actgaccgtc atcaaagcca
     6841 gcggcatgag caccaaaaag ctgctgttga ttctgtcgca gttcggtttt atttttgcta
     6901 ttgccaccgt cgcgctcggc gaatgggttg cgcccacact gagccaaaaa gccgaaaaca
     6961 tcaaagccgc cgccatcaac ggcaaaatca gcaccggcaa taccggcctt tggctgaaag
     7021 aaaaaaacag cattatcaat gtgcgcgaaa tgttgcccga ccatacgctt ttgggcatca
     7081 aaatttgggc gcgcaacgat aaaaacgaat tggcagaggc agtggaagcc gattccgccg
     7141 ttttgaacag cgacggcagt tggcagttga aaaacatccg ccgcagcacg cttggcgaag
     7201 acaaagtcga ggtctctatt gcggctgaag aaaactggcc gatttccgtc aaacgcaacc
     7261 tgatggacgt attgctcgtc aaacccgacc aaatgtccgt cggcgaactg accacctaca
     7321 tccgccacct ccaaaacaac agccaaaaca cccgaatcta cgccatcgca tggtggcgca
     7381 aattggttta ccccgccgca gcctgggtga tggcgctcgt cgcctttgcc tttaccccgc
     7441 aaaccacccg ccacggcaat atgggcttaa aactcttcgg cggcatctgt ctcggattgc
     7501 tgttccacct tgccggacgg ctcttcgggt ttaccagcca actctacggc atcccgccct
     7561 tcctcgccgg cgcactacct accatagcct tcgccttgct cgccgtttgg ctgatacgca
     7621 aacaggaaaa acgttgaacc aatgccgtct gaacctctct tcagacggca tttgttttca
     7681 ttgacacatt cccacagaca gatagccgtt ccctattaca ttacctgtca taacagttcc
     7741 atttttgtta aaactagtct atgatagcgg tacaaatatt gtttacaata tttaacgcaa
     7801 atcatttgca acccgacaaa agaaaaacag aaaaaggaac aaagagatgt tagaagccta
     7861 tcgtaaagcc gccgccgagc gcgccgccct cggcattccc gccctccctt tgaacgcgca
     7921 gcaaaccgcc gatttggttg agctgctgaa aagcccgccc gcaggcgaag gcgagttctt
     7981 ggtcgaactg cttgcccacc gtgttccgcc cggtgtggac gatgccgcca aagtcaaagc
     8041 ctcattcctg gctgccgttg ccgaaggcag cgcgtccagc ccgctgatct cccccgaata
     8101 tgcgaccgaa ctcttaggta caatgctcgg cggttacaat attcacgcct taatcgaact
     8161 cttggacgac gacaaactcg cgtccattgc tgccaaaggc ttgaaacata cgcttctgat
     8221 gttcgattcc ttccacgacg ttcaagaaaa agccgaaaaa ggcaacaaat acgcgcaaga
     8281 agttttgcaa tcttgggcag atgccgaatg gttcgcctca cgcgccaaag ttcccgaaaa
     8341 aatcaccgtt accgttttca aagttgacgg cgaaaccaat acagacgacc tctcccccgc
     8401 gcccgacgcg tggagtcgtc ccgatattcc gctgcacgcg ctggccatgc tgaaaaaccc
     8461 gcgcgacggc atcacgcccg acaaaccggg cgaagtcggt ccgattaaat tgttggaaga
     8521 actcaaagcc aaaggccatc cggttgctta cgtcggcgac gtggtcggta ctggttcttc
     8581 acgcaaatcc gcgaccaact ccgtcatttg gcataccggc gaagacattc cgttcgtgcc
     8641 gaacaaacgc ttcggcggcg tatgtttggg cggcaaaatc gcgccgattt tcttcaatac
     8701 ccaagaagat tccggcgcgc tgccgattga agtcgatgta tctgctctaa aaatgggcga
     8761 tgtcgtcgat atcctgcctt atgaaggcaa aatcgtgaaa aacggcgaga ctgttgccga
     8821 gtttgaattg aaatcacaag tattgctgga cgaagtgcaa gccggcggcc gtatcaacct
     8881 gattatcggc cgaggtctga ccgccaaagc gcgcgaagcc ctgaaactgc ctgcctctac
     8941 tgcattccgc ctgccgcaag cgcctgccga aagcaaagcc ggtttcacct tggcgcaaaa
     9001 aatggtcggc cgcgcctgcg gtctgcccga aggacaaggc gtgcgcccgg gtacttactg
     9061 cgaaccgcgt atgacgacgg tcggctcgca agacacgacc ggcccgatga cccgcgacga
     9121 gttgaaagac ttggcttgtt tgggcttctc cgccgatatg gtgatgcagt ctttctgcca
     9181 caccgccgcc tatccgaaac ctgtcgatgt aaaaacccat aaagaactgc ccgcctttat
     9241 ttccacccgt ggcggcgtgt cactgcgtcc gggcgacggc gtcatccact cgtggctcaa
     9301 ccgcctgctg ctgcccgata ccgtcggcac cggcggcgac agccataccc gtttccccat
     9361 cggtatttcc ttccccgccg gctccggctt ggttgccttt gccgccgcaa cgggcgtaat
     9421 gccgctcgat atgcccgagt ctgtattggt acgcttcagc ggcaagctgc aaccgggcgt
     9481 aaccctgcgc gatttggtga acgccatccc gctgtacgca atcaaacaag gtttgctgac
     9541 cgttgccaaa gccggtaaga aaaacatctt ctccggccgc atcctcgaaa tcgaaggcct
     9601 gcctgatttg aaagtggaac aagcctttga attgaccgac gcatccgccg aacgctccgc
     9661 cgccggctgt accgtgaagc tcaacaaaga gccgattatc gagtacatga aatccaacgt
     9721 cgtgttgatg aaaaacatga ttgccaacgg ctatcaagac ccgcgcactt tggaacgccg
     9781 catcaaagct atggaaaaat ggctggcaaa tcccgagttg ctcgaagcgg ataaagatgc
     9841 cgaatacgcc gccgtgattg aaatcaacat ggacgacatc aaagagccga ttatcgcctg
     9901 cccgaacgac ccggacgacg tgtgcttcat gtccgaacgc tccggcacca aaatcgacga
     9961 agtattcatc ggttcgtgta tgaccaacat cggccacttc cgcgccgcct ccaaactttt
    10021 ggaaggcaag gcagacaccc ccgtccgcct gtggattgcg ccgccgacca aaatggacgc
    10081 gaaacaattg tccgacgaag gacactacgg cgtactcgga cgtgccggcg cgcgtatgga
    10141 aatgccgggt tgctccttat gtatgggtaa tcaggcgcaa gtacgcgaag gtgcgaccgt
    10201 tatgtccacc tccacccgca acttcccgaa ccgtttgggt aaaaacacct ttgtttacct
    10261 cggttcggcg gaattggcag cgatttgctc caaactgggt aaaatcccga ccgttgaaga
    10321 atatcaagcc aatatcggca tcatcaacga acagggcgat aaaatctacc gctatatgaa
    10381 cttcaacgaa atcgacagct acaacgaagt agccgagacc gtgaacgttt aatccccgtc
    10441 atccgtatga agtaagggat tgaccgcaat gccgtctgaa caaccttcag acggcattgc
    10501 aacattccgc taacccttct ttccgcaaac gctgcaaata cggcgttcac gcccccacat
    10561 aaaggaaacg acagtgaacc tgaaaaaccg ccattttctg aaacttttag acttcacgcc
    10621 ggaagaaatc accgcctacc tcgaccttgc cgccgaattg aaagccgcca aaaaagcagg
    10681 gcgcgagatt cagcggatga aagggaaaaa catcgccctg atttttgaaa aaacctctac
    10741 tcggacgcgc tgcgcgtttg aagtcgccgc gcgcgatcaa ggcgcgggag tgacttattt
    10801 agaaccgtcc gccagccaaa tcgggcataa ggaaagcatc aaagacaccg cccgcgtgtt
    10861 gggcaggatg tacgatgcca tcgaatatcg cggtttcggt caggaagttg ttgaagaatt
    10921 ggcgaaatac gcgggcgtac ccgtgttcaa cgggctgacc aacgagttcc atcccacaca
    10981 aatgcttgcc gacgcactga ctatgcgcga acacagcggc aaacctttga accaaaccgc
    11041 gtttgcctac gtcggcgacg cgcgttacaa catgggcaat tccctgctga ttttaggggc
    11101 aaaattgggg atggacgtgc gtatcggcgc accgcaaagc ctgtggccgt ctgaaggcat
    11161 tattgccgcc gcacacgccg ccgccaaaga aaccggcgca aaaattaccc tgaccgaaaa
    11221 cgcgcatgaa gccgtgaaga atgttgattt tattcatacc gatgtgtggg tcagcatggg
    11281 cgagccgaaa gaagtctggc aggaacgcat cgatttgctg aaagattacc gcgttacgcc
    11341 cgaactgatg gcggcatcgg gcaatccgca agtcaaattc atgcactgcc tgcccgcctt
    11401 ccacaaccgc gaaaccaaag tcggcgaatg gatttacgaa accttcgggc tgaacggtgt
    11461 ggaagttaca gaagaaatat tcgaaagccc cgccagcatc gtgttcgatc aggcggaaaa
    11521 ccgtatgcac acgattaaag cggtaatggt cgcggctctg ggcgactgac agaactgtgc
    11581 ctgtttaaat tcatccgcaa cacagatacc gtctgaacac gatgttcaga cggtatc
//
LOCUS       DAWGRZ010000046        11519 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000046 DAWGRZ010000000
VERSION     DAWGRZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11519
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.046"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            207..1049
                     /locus_tag="WHT44_000899"
     CDS             207..1049
                     /locus_tag="WHT44_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ2533105.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVIAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVAV"
     gene            1079..1543
                     /gene="nrdR"
                     /locus_tag="WHT44_000900"
     CDS             1079..1543
                     /gene="nrdR"
                     /locus_tag="WHT44_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ2533106.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPNCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSAALADMVLKELLRQDTEAAVRFAALHKRFDNPADFASWLAQAVKTGGKA"
     gene            1575..2684
                     /gene="ribD"
                     /locus_tag="WHT44_000901"
     CDS             1575..2684
                     /gene="ribD"
                     /locus_tag="WHT44_000901"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ2533107.1"
                     /translation="MFSDTDISMMENALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALVRAGVSRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPPNSHLVTDGQSPTYIATLERNEDRLHPYREHAHVRILMPSETADSK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGSGKDLFSLL
                     ENRAALSAPPLWTPVSSEILGHNIKTVFRKNGNAF"
     gene            2879..4300
                     /locus_tag="WHT44_000902"
     CDS             2879..4300
                     /locus_tag="WHT44_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ2533108.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYATADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRHAPFSPAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEENAPPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLAEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACAASFW
                     LFFAFKTESSCRLWQPLKRLPLYLHTLFCLTSSAAYTCFGTPANYPLFAGVWAAYLAG
                     CILRHRKDLHKLFHYLKKQGFPL"
     gene            4468..5709
                     /locus_tag="WHT44_000903"
     CDS             4468..5709
                     /locus_tag="WHT44_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HEZ2533109.1"
                     /translation="MSKFFKRLFDIVASASGLIFLSPVFLILIYLIRKNLGSPVFFFQ
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDGERLTPFGKKLRAASLDELPELWNILK
                     GEMSLVGPRPLLMQYLPLYDNFQNRRHEMKPGITGWAQVNGRNALSWDEKFACDVWYI
                     DHFSLCLDIKILLLTVKKVLIKEGISAQGEATMPPFTGKRKLAVVGAGGHGKVVADLA
                     AALGRYREIVFLDDRAQGSVNGFSVIGTTLLLENSLSPEQYDVAVAVGNNRIRRQIAE
                     KAAALGFALPVLVHPDATVSPSATVGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHD
                     CLLNAFVHISPGAHLSGNTHIGEESWIGTGACSRQQIRIGSRATIGAGAVVVRDVSDG
                     MTVAGNPAKPLPRKNPETSTA"
     gene            5842..7017
                     /locus_tag="WHT44_000904"
     CDS             5842..7017
                     /locus_tag="WHT44_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ2533110.1"
                     /translation="MLNTFLSPWPCFTQEEADAVSKVLLSNKVNYWTGNECREFEKEF
                     AAFAGTRYAVALANGTLALDVALKAMGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAALTPTTKAVIVVHLAGMPAEMDGIMALAKEHNLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYNHEHAPGFRWLHESFGTNWRMMEMQAVIGRIQLKRLPEWTARRRENAAKLAESLG
                     KFSSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVGELNARKVPCYQGSCSEV
                     YLEKAFDNTPWRPKERLTNAVELGDTSLMFLVHPTLTDDEIAFCKKHIEAVLTEAAR"
     gene            7065..8975
                     /gene="pglD"
                     /locus_tag="WHT44_000905"
     CDS             7065..8975
                     /gene="pglD"
                     /locus_tag="WHT44_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ2533111.1"
                     /translation="MNLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSDEW
                     FDFANWQSFLLTALLTITLFIRMGLYHAVTRFVSFRILTTALAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDNRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATTSGVRTFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPEITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPNTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TAHPRIMTADEAMLPWHELSVLLDRIRSACDRYDQQAIRTLLINAPTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            9031..10323
                     /locus_tag="WHT44_000906"
     CDS             9031..10323
                     /locus_tag="WHT44_000906"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ2533112.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFNLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDSEMARLD
                     ALAQEHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTR
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(10375..11211)
                     /locus_tag="WHT44_000907"
     CDS             complement(10375..11211)
                     /locus_tag="WHT44_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ2533113.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADKLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRKGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIQAGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RY"
BASE COUNT         2554 a         3682 c         2958 g         2325 t
ORIGIN      
        1 ctcaaaagtt acggaacgaa aaacaaccaa aaccggacaa gtcggattcc cgcctgcgcg
       61 ggaatgacgg aatcttaagt ttccgtcttt gttttctgtt ttctgttttc gagggaataa
      121 tggggaacaa gccgtatttc agacggcatt ttcagttcgg ggtataatcc gaatacttgc
      181 gaccatctga atcattggga caaaccatgt gtcaactgct gggcatgaac tgcaatacgc
      241 cgaccgatat tatgttttcc tttgaaggct tccgccgcag gggcggcatt accgaccacc
      301 atgccgacgg tttcggtatc ggctttttcg aaggcaaagg cgtgcgcctg ttccacgacg
      361 acaagccgag cgcaaattcc cccgtcgccg acctcgtgcg tgcctaccaa atcaaatcgg
      421 aaaacgtcat cgcacatatc cgcaaagcat cgcaaggaca aacctcgctg gcgaacaccc
      481 atccctttat gcgtgaaatg tggggcggct actggctgtt tgcccacaac ggacatttga
      541 ttgatttttt ccccgaacag ggcgaatttt tccaccccgt cggcacaacc gattccgaac
      601 gcgcgttctg ccacatcctc aaccgcctgc gcacccgctt tgccgcccgt cccgacgacg
      661 acacgctgtt tgacgcgatt gcggggctga cgcacgaaat ccgcaagttc gggctgttta
      721 actttatgct ttcagacggc attgccctgt ttgcccacgc cagcacgctg ctgcactaca
      781 tcgtccgcca agccccgttc ggcaaggcgc gcctgctcga cgacgacgtg atggtcgatt
      841 ttgccgaagt aaccacgccc tccgaccgcg tcgccgttat cgccaccctg ccactgaccc
      901 gcgacgaatc atggtcccaa cttgccgtgg acgaactggt catgttccgc gaaggcaaca
      961 tcgtccgaca cgaccgtccc gaaaaccccg tctatatgag tgccgaagaa ggtctggaaa
     1021 tcgcccgcgc cgccggcgtc gccgtctgaa cttcagacga cataggagga cgaacccgat
     1081 gaaatgcccg ttttgcgccc accccgacac ccgcgttgcc gattcgcgtc tgatggaaga
     1141 acgcaacgcc gtgcgccgcc gccgccactg ccccaactgc ggcaaacgct tcggcacgct
     1201 cgaaaccgcc gaactcaaaa tgcccgccgt catcggtccg gacaaaaaac gctcgccctt
     1261 taatgcacaa cgcctccgca acgacctgac cgccgccgcc cgaaaatccg ccctgacacc
     1321 cgaacagatc gacgaaaccg tccgcctgac ggaacacagg ctctacactt cgggtcagcg
     1381 cgacatcccc tctgccgcac ttgccgacat ggtgctaaaa gaactgctcc gacaagatac
     1441 ggaagccgcc gtccgtttcg ccgccctgca caaacgcttc gacaatccgg cagactttgc
     1501 ctcgtggctg gcgcaagccg tcaaaacagg cggcaaagcc tgattccccc aacccatact
     1561 gatacggtat ccctatgttt tcggacacag atatatccat gatggaaaac gccctccgac
     1621 ttgccgcttt ggggcgtttt tccacttcgc ccaatccgcg cgtcggctgc gttatcgcac
     1681 acggcagcca aattgtcggg caaggcttcc acgtcaaagc gggcgaaccc catgccgaag
     1741 tccacgccct gcgtcaggcg ggcgaaatgg cacaaggcgc gaccgccttt gttaccctcg
     1801 aaccgtgcag ccattacggg cgcacaccgc cctgtgccga agcactggtg cgggcgggcg
     1861 tgtcccgcgt cgttgccgcc atgcgcgacc ccaacccgct ggttgcaggc aaagggcttg
     1921 ccctgctcga agcagcaggc atcaagacgg aatgcggttt actcgaacat caggcaaggg
     1981 aactcaaccg aggcttcctg tcgcgcatcg aacgccgccg cccctttgtc cgcctcaaat
     2041 gcgccgtttc gctggacggc aaaaccgccc tttcagacgg cagcagcttt tggattaccg
     2101 gcgaagacgc gcgtgccgac gtacaggttt tgcgtgccga aagctgcgcg gtgctgaccg
     2161 gcatcggcac ggtgttggcg gacaatcccc ggctcaacgt ccgcgctttt ccaactttgc
     2221 gccaacccgc acgcatcgtt ttagacagcc gcctgcgcct gcccccgaac agccatttgg
     2281 ttaccgacgg acaatctccg acctacatcg ccacactcga acgcaacgaa gacagactgc
     2341 acccctatcg ggaacacgca cacgtccgca tcctgatgcc gtctgaaacg gcagacagca
     2401 aaatcgacct gcaccacctg atgcgcctcc ttgctgacga aggtttcggc gaaatcatgg
     2461 tcgaagcagg ctccgaactc acatccgcat ttttggcaga aaatctggca gacgaaatcg
     2521 tcctgtaccg ttcgcccaaa atcctcggca gcggcaaaga cctgttttcc ctgctcgaaa
     2581 accgcgccgc cctttccgca ccgcccttgt ggacacccgt ttcaagcgaa atcctcggac
     2641 acaacatcaa aaccgtgttc cgaaaaaacg gcaacgcctt ttaaagggtt tgcgccgttt
     2701 cactatataa taacgccgat aaaaaacggc cccgttcaga ccgccgcccg cccgaaaaaa
     2761 cgcaacccgg actgccgcac cccgccggca gccgcgacgg tctgaaagcc gtcaaattcc
     2821 gatcaagaaa ggcttcagac ggcacaggca gcatcccgcc gccgcccgga catcaaaaat
     2881 ggacacaaaa gaaatcctcg gctacgcggc aggctcgatc ggcagcgcgg ttttagccgt
     2941 catcatcctg ccgctgctgt cgtggtattt ccccgccgac gacatcgggc gcatcgtgct
     3001 gatgcagacg gcggcggggc tgacggtgtc ggtgttgtgc ctcgggctgg atcaggcata
     3061 cgtccgcgaa tactatgcca ccgccgacaa agacaccttg ttcaaaaccc tgttcctgcc
     3121 gccgctgctg tctgccgccg cgatagccgc cctgctgctt tcccgcccgt ccctgccgtc
     3181 tgaaatcctg ttttcactcg acgatgccgc cgccggcatc gggctggtgc tgtttgaact
     3241 gagcttcctg cccatccgct ttctcttact ggttttgcgt atggaaggac gcgcccttgc
     3301 cttttcgtcc gcgcaactcg tgcccaagct cgccatcctg ctgctgctgc cgctgacggt
     3361 cgggctgctg cactttccag cgaacaccgc cgtcctgacc gccgtttacg cgctggcaaa
     3421 ccttgccgcc gccgcctttt tgctgtttca aaaccgatgc cgtctgaagg ccgtccggca
     3481 cgcaccgttt tcgcccgccg tcctgcaccg ggggctgcgc tacggcatac cgatcgcact
     3541 gagcagcatc gcctattggg ggctggcatc cgccgaccgt ttgttcctga aaaaatatgc
     3601 cggcctggaa cagctcggcg tttattcgat gggtatttcg ttcggcgggg cggcattatt
     3661 gttccaaagc atcttttcaa cggtctggac accgtatatt ttccgcgcaa tcgaagaaaa
     3721 cgccccgccc gcccgcctct cggcaacggc agaatccgcc gccgccctgc ttgcctccgc
     3781 cctctgcctg accggcattt tctcgcccct tgcctccctc ctgctgccgg aaaactacgc
     3841 cgccgtccgg tttatcgtcg tatcgtgtat gctgccgccg ctgttttgca cgctggcgga
     3901 aatcagcggc atcggtttga acgtcgtccg caaaacgcgc ccgatcgcgc tcgccacctt
     3961 gggcgcgctg gcggcaaacc tgctgctgct ggggcttgcc gtgccgtccg gcggcgcgcg
     4021 cggcgcggcg gttgcctgtg ccgcctcatt ctggctgttt tttgccttca agaccgaaag
     4081 ctcctgccgc ctgtggcagc cgctcaaacg cctgccgctt tatctgcaca cattgttctg
     4141 cctgacctcc tcggcggcct acacctgctt cggcacgccg gcaaactatc ccctgtttgc
     4201 cggcgtatgg gcggcatatc tggcaggctg catcctgcgc caccggaaag atttgcacaa
     4261 actgtttcat tatttgaaaa aacaaggttt cccattatga aaatcgtttt gaccacatct
     4321 atggcaggct tgggcggcac gggcacgata tcatcgattg ccaaatggcg tgcaaaaacg
     4381 accccgttca gtccgacgaa atcgtccgcc gcttcaggcg cgacatttcc tatcggaaaa
     4441 tcgtcaacct gattgaaaga ttggcaaatg agtaaattct tcaaacgcct gtttgacatt
     4501 gttgcctccg cctcgggact gattttcctc tcgccagtat ttttgatttt gatatacctc
     4561 atccgcaaga atctaggttc gcccgtcttc ttctttcagg aacgccccgg aaaggacgga
     4621 aaacctttta aaatggtcaa attccgttcc atgcgcgacg cgcttgattc agacggcatt
     4681 ccgctgcccg acggagaacg cctgacaccg ttcggcaaaa aactgcgtgc cgccagtttg
     4741 gacgaactgc ctgaattatg gaatatctta aaaggcgaga tgagcctggt cggcccccgc
     4801 ccgctgctga tgcaatatct gccgctgtac gacaacttcc aaaaccgccg ccacgaaatg
     4861 aaacccggca ttaccggctg ggcgcaggtc aacgggcgca acgcgctttc gtgggacgaa
     4921 aaattcgcct gcgatgtttg gtatatcgac cacttcagcc tgtgcctcga catcaaaatc
     4981 ctactgctga cggttaaaaa agtattaatc aaggaaggga tttccgcaca gggcgaagcc
     5041 accatgcccc ctttcacagg aaaacgcaaa ctcgccgtcg tcggtgcggg cggacacgga
     5101 aaagtcgttg ccgaccttgc cgccgcactc ggccggtaca gggaaatcgt ttttctggac
     5161 gaccgcgcac aaggcagcgt caacggcttt tccgtcatcg gcacgacgct gctgcttgaa
     5221 aacagtttat cgcccgaaca atacgacgtc gccgtcgccg tcggcaacaa ccgcatccgc
     5281 cgccaaatcg ccgaaaaagc cgccgcgctc ggcttcgccc tgcccgttct ggttcatccg
     5341 gacgcgaccg tctcgccttc tgcaacagtc ggacaaggca gcgtcgttat ggcgaaagcc
     5401 gtcgtacagg caggcagcgt attgaaagac ggcgtgattg tgaacactgc cgccaccgtc
     5461 gatcacgact gcctgcttaa cgctttcgtc cacatcagcc caggcgcgca cctgtcgggc
     5521 aacacgcata tcggcgaaga aagctggata ggcacgggcg cgtgcagccg ccagcagatc
     5581 cgtatcggca gccgcgcaac cattggagcg ggcgcagtcg tcgtacgcga cgtttcagac
     5641 ggcatgaccg tcgcgggcaa tccggcaaag ccgctgccgc gcaaaaaccc cgagacctcg
     5701 acagcataag cgattaaaat acacccccgt acagaccgat tttgacaaca cctgcggcgc
     5761 gcgcccgatt cttcggaaca cgcccccctt cagacggcat agggtcggaa atgccgtctg
     5821 aaaaccgacg gacaaaccat catgctgaac actttccttt ccccgtggcc ctgcttcacc
     5881 caagaagaag ccgatgccgt ttccaaagtc ctgctgtcca acaaagtcaa ctactggacg
     5941 ggcaacgaat gccgcgaatt tgaaaaagaa tttgccgcct ttgccggcac gcggtacgcc
     6001 gtcgcccttg ccaacggcac gctggcactc gatgtcgcgc tcaaagcaat gggcataggc
     6061 gcgggcgacg atgtgattgt tacctcgcgc accttcctcg cttccgcgtc ctgcattgtg
     6121 aacgcgggcg caaaccccgt gtttgccgat gtggatttga acagccaaaa catcagcgcg
     6181 gaaaccgtca aagccgcgct gacaccgact accaaagccg tcatcgtcgt ccacctcgcc
     6241 ggtatgcccg ccgaaatgga cggcattatg gctttggcaa aagaacataa tctttgggta
     6301 atcgaagact gcgcccaagc gcacggcgca aaatacaaag gcaaatccgt cggctctatc
     6361 ggacacgtcg gcgcgtggtc gttctgccaa gacaaaatca tgaccaccgg cggcgaaggc
     6421 ggtatggtta cgaccaacga caaaaccctg tgggaaaaaa tgtggtcgta caaagaccac
     6481 ggcaaaagct acgatgccgt gtacaaccac gaacacgcgc ccggtttccg ctggctgcac
     6541 gaaagtttcg gcacaaactg gcgtatgatg gaaatgcagg cggtcatcgg acgcatccag
     6601 ctcaaacgcc tgcccgaatg gacggcgcgc cgccgagaaa acgccgccaa gctggcggaa
     6661 agtttgggca aattcagcag catccgcttg gttgaagtcg ccgactacat cggacacgcg
     6721 caatataagt tctacgcctt cgtcaaaccc gaacacctca aagacggctg gacgcgcgac
     6781 cgcatcgtcg gcgaactgaa cgcgcgcaaa gtcccctgct atcaaggcag ctgctccgaa
     6841 gtctatttgg aaaaagcctt cgacaacacg ccgtggcgac cgaaagagcg tttgacaaat
     6901 gctgtcgagt tgggcgacac cagcctgatg ttcttggtgc acccgacgct gaccgacgac
     6961 gaaattgcgt tttgcaaaaa acacatcgaa gccgtcttga ccgaagccgc acgataaccc
     7021 ttcagacggc atatgccgcc tgaaaacaca taccgcccca cgatatgaat ctggaaactc
     7081 tgatcgccct gccgcgcaac atcaagaaaa tctgtttcct catacacgat tttctgatga
     7141 ttttcattgc cttttggttc acccaaagcc taaaggccga ctactcggac gaatggttcg
     7201 attttgccaa ctggcagtct tttttgctga ctgccttgct gaccatcaca ttatttatcc
     7261 gaatggggct ttaccacgcc gttacacgct tcgtcagctt ccgcatcctc accaccgcac
     7321 tggcgggcag cctcgcctcc gccgtgttgt ttttcctcaa tacgctgata tttgaagaaa
     7381 ggctgcgcct cgccctgccg attgtctatt tcttactgct gtttgtttcc gtgaccggct
     7441 cgcgtatggt tttgcgcgga ctgttgtccg aacaccccaa aaaacagatg atccctgtca
     7501 tcatttacgg cgcgggacgg tcgggcagac aactgcttga ggccgtcaaa caaatgcgcg
     7561 aatattccgc cgccgccttt gtagacgacg accccaaact gtggcacacc gtcatctacg
     7621 accttgccgt ttaccagccc gatgccatcg ccttcctcat cgaacgctac ggcgtggaaa
     7681 aaatcctgct cgccatcccc ggcgcgaccc aggaacaacg ccgccgaatc atcaacaaac
     7741 tggaagccta tccgtgcgaa gtgttgacca ttcccggaat gaaagacctg atggacggaa
     7801 aaatcagcat cggcacgctc aaaaaaatct ctgtgtccga cctgctcggg cgtgattccg
     7861 tcgcgcccga caaccgcctg atgagtgccg acatcgaagg caaaaccgtc atggtaaccg
     7921 gcgcgggcgg ctccatcggt tcggaactct gccgccagat tatccgccgc cgccccgaaa
     7981 agctgctgct gttcgagtta tccgaattcg ccctgtacgc catcgaaaaa gaattgcgcg
     8041 aaacctgcat ccaaaaacgc ctcgacaccg aaatcctgcc ctttctcggt tcggtgcaaa
     8101 accgcacgct gctcgaacac gtcatgaccg ccttttccgt tgcgaccgtc tatcacgccg
     8161 ctgcctacaa acacgtcccc atggtcgagt tcaacaccgt cgaaggcata cgcaacaaca
     8221 tcttcggcac actcgagtgc gcgcttgccg ccacgacatc gggcgtaaga actttcgtcc
     8281 tcatctccac cgacaaagcc gtccgcccca ccaacaccat gggtgccagc aaacgcatgg
     8341 cggaactctg ccttcaggca ctcgccgccg aacccggaca aaaaacccgc ttcagcatgg
     8401 tacgtttcgg caatgtttta ggttcgtccg gctccgttgt cccgctgttt gaaaaacaga
     8461 ttgcagaagg cggcccgctt accctgaccc accccgaaat cacacgttat ttcatgacca
     8521 tacccgaagc cgcccaactc gtcatacagg caggcgcgat gggtacgggc ggcgacgtat
     8581 tcgtcctcga catgggtgaa tccgtcaaaa tcatcgacct tgcccgccaa atgattaccc
     8641 taagcggcct caaacccaac acacccgaac aacccgacgg cgacatcgaa atcctcatta
     8701 ccggactgcg tcccggcgaa aaactctacg aagagctgct catcggcgac aacgtccgca
     8761 aaaccgccca tccgcgcatt atgacggctg acgaggcgat gctgccgtgg cacgagctct
     8821 ctgtcctgct cgaccgcatc cgttcggcct gcgaccgtta cgaccagcag gcaatccgca
     8881 ccctgctcat caacgccccg accggctttg ccccgagcga cggcatctgc gacctgcttt
     8941 gggtacgaga aacacacaga aaaaatgccg tctgaacctt cagacggcat aacgtacaaa
     9001 ccaacctacc ttacacacac ggagtttgac atgcagttct cagcattcgg cgaaaaattc
     9061 acgcaacaca gcggcatcct ccaactgatg gacgacctcg gcgacgcgct caaaagcgac
     9121 aagcccgtca acatgctcgg cggcggcaat ccggcgcgca ttccggaaat cgatcgggcg
     9181 ttcgccgaca tattctccaa actggcggcg gaacacgctg tcgagaacat cggcaactac
     9241 tccaatcccc aaggcgatgc cgcgctgatt gacgcgctga ccgccttcct caaccgcgaa
     9301 tacggctgga acctgaccgc cgacaatatc gcgctgacca acggttcgca aaacgcgttt
     9361 ttctatttat tcaacctctt cggcggcaaa ttcaaccttt cagacggcac atccgcagaa
     9421 aaagccattt tgttgccgct cgcgcccgaa tacatcggct atgccgacgt gcatgtcgaa
     9481 gggcagcact tcgtttccgt caagcccaaa atcgaaaacg tcgaacacga aggcgaagca
     9541 ggcttcttca aataccgcgt ggactttgac gcactggaaa acctgcccga actcaaagcg
     9601 ggcaaaatcg gcgcgatttg ctgttcgcgc ccgaccaacc cgaccggcaa cgtgctgacc
     9661 gacagcgaaa tggcgcggct ggacgctttg gcgcaagaac acggcattcc gctgattatc
     9721 gacaacgcct acggaatgcc gttccccaac atcatttaca gcgacgtaac gctgaattgg
     9781 catgaaaaca tcatcctctg cttcagcctt tccaaagtcg gtctgccggg cgttcgcacc
     9841 ggcatcatcg tcgccgcgcc cgaagtcgtc aaagccgtca gcagcctgaa cgcgattgtg
     9901 aaccttgccc ccacgcgctt cggcgcggcc atcgccgccc cgctgctgga aagcggcgag
     9961 atgaaacggc ttgccgacca agtcatccgg ccgttttacc gcaatcaggc gcaaaccgcc
    10021 gtctcgctgc tcaagcgcga gctgggcgcg tacccgatga aaatccacaa acccgaaggc
    10081 gcgattttcc tgtggctctg gtttgaaaac ctgcccgttt cttcgcaaac cctgtacgaa
    10141 atgctcaaag ccgaaggtac gctgattatt ccgggcgagc atttcttcgt cggcatcgac
    10201 acgcgggatt acccgcacgc gggcgagtgc atccgcatga gcatcgcgca ggacgctcaa
    10261 acgctggaaa aaggcatcgc cgccatcggc aaaaccgtcc gaaaactgta cgacaacgtt
    10321 taaaccgcaa aaaatgccgt ctgaaaggtt ttcagacggc atttttatct gcattcaata
    10381 tcgggaaaaa tgcgcccaca ccggcctgca gttttccggc agttcgggac acgcgccgag
    10441 aatgccgccg ctaaagtcgt ttaaacggtg gaagtcgctg cccgcgctgg cgagcatacc
    10501 gaagcgttct gccaaaagcg cgtagttgag gcggtcgttt ttgcagcagt tgccgctgtg
    10561 gacttcgatg cctgcgccgc cgaggttttt aaattcttca aacaaattgc gcttggcggt
    10621 ggcggacaaa tcgtagcgca tggggtgggc gatgactgcc atgccgcccg ctccgttgac
    10681 ggcggagacg cagtcttcca gcgtcgccca ttcgtggcgg acggcgcagg atttgccgtc
    10741 gcccaagtat ttggtgaacg cctgctgctt gtttttgacg tgtcccgctt ggatgaggaa
    10801 ctcggcgacg tgggtgcggc tgaccatttc tttgtttgcc gccagtgcca gcgcgccgtc
    10861 gtatgcgccg ccgatgcctt tcttttcgag cttggcggcg atggcttcaa gacgtttcag
    10921 acggcctttc cgcacttgcg ccaacaggtt ttgcaggttt tcgtcctgct cgtcgaaatc
    10981 caaaccgaca acgtgtatgg tgcgcccgcg ccacgttacg gagatttcca caccgttaat
    11041 caggcgcaaa ccgagcttgt cggcttcggc gcgcgcttcg gcgatgccgc cggtgtggtc
    11101 gtggtcggtc aacgccagca gcgtgcagcc gttttgatgc gcgaggcgca cgacttcggc
    11161 gggggagagc ataccgtcgg aaacggtgga atggcagtgc aggtctatca tggggtgtta
    11221 tgtgtgttgt gaatgaaggt cgggggttaa tattatcggt tggtgtggat acagcggtga
    11281 tttcaacaaa caggtgtatg gcaaatgcaa aggaaaagtc cctatgccgt cattcccgcg
    11341 caggcgggaa tccagacctt gatttgtcaa aaatatttaa ggttaaccgc tatttcgaac
    11401 ttccggattc ccgcctgcgc gggaatgacg atatggacgt tttcagtttt aatctactat
    11461 aaaagactgt ctgaaaacgt ggttttatag tgaattaaat ttaaaccggt acagcgttg
//
LOCUS       DAWGRZ010000047        11417 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000047 DAWGRZ010000000
VERSION     DAWGRZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11417
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.047"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(12..1307)
                     /gene="tyrS"
                     /locus_tag="WHT44_000908"
     CDS             complement(12..1307)
                     /gene="tyrS"
                     /locus_tag="WHT44_000908"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ2533114.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     NQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLHQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLNGEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(1370..2987)
                     /locus_tag="WHT44_000909"
                     /pseudo
     CDS             complement(1370..2987)
                     /locus_tag="WHT44_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            complement(2941..3238)
                     /locus_tag="WHT44_000910"
                     /pseudo
     CDS             complement(2941..3238)
                     /locus_tag="WHT44_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            3373..3621
                     /locus_tag="WHT44_000911"
     CDS             3373..3621
                     /locus_tag="WHT44_000911"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533115.1"
                     /translation="MPSERSFRIGDRDIRNAGRLPVTAGQAVCFAGIGEGKSEMRVGV
                     YFDAAAFRRYGKRRKSSKSASDGISGKPPETCRIWVTR"
     gene            complement(3611..4702)
                     /gene="ychF"
                     /locus_tag="WHT44_000912"
     CDS             complement(3611..4702)
                     /gene="ychF"
                     /locus_tag="WHT44_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ2533116.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(4817..6493)
                     /locus_tag="WHT44_000913"
     CDS             complement(4817..6493)
                     /locus_tag="WHT44_000913"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ2533117.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKANGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLDALEKGLPNLLKHISNLKNVFGLPVVVALNRFVSDADAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIESQTNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEDF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDAEGVIHGLF"
     gene            6668..7099
                     /locus_tag="WHT44_000914"
     CDS             6668..7099
                     /locus_tag="WHT44_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ2533118.1"
                     /translation="MGSNAWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALLLFLTYTGKWQGVNGFTGRNWTFLILSGLATGASWLAYFKALQLGNASQVAPID
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            complement(7133..7828)
                     /locus_tag="WHT44_000915"
     CDS             complement(7133..7828)
                     /locus_tag="WHT44_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ2533119.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQTASSLPEHISAHLWLVENPPHNPDGDFSLLPDSSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(7844..8344)
                     /gene="hpaC"
                     /locus_tag="WHT44_000916"
     CDS             complement(7844..8344)
                     /gene="hpaC"
                     /locus_tag="WHT44_000916"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ2533120.1"
                     /translation="MADLQKNFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            complement(8459..8899)
                     /gene="nadR"
                     /locus_tag="WHT44_000917"
     CDS             complement(8459..8899)
                     /gene="nadR"
                     /locus_tag="WHT44_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ2533121.1"
                     /translation="MPTQSKHASINIGLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(9240..9749)
                     /locus_tag="WHT44_000918"
     CDS             complement(9240..9749)
                     /locus_tag="WHT44_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ2533122.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSSA
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            complement(9828..9903)
                     /locus_tag="WHT44_000919"
     tRNA            complement(9828..9903)
                     /locus_tag="WHT44_000919"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9868..9870),aa:Lys,seq:ttt)
     gene            10119..11198
                     /gene="apbC"
                     /locus_tag="WHT44_000920"
     CDS             10119..11198
                     /gene="apbC"
                     /locus_tag="WHT44_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ2533123.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPETGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIAKIYTDA
                     AFQIALSIADKGKDFSSRFPKIVVE"
BASE COUNT         2640 a         3140 c         3044 g         2593 t
ORIGIN      
        1 cggcaatcgg attatttcca aaccaaaagc gcgtggttgc gtttgccgcg ccgaaggata
       61 gtgtatttgc cgaaacgttt gtgttcgccg ttcagcaggc aggcatcgtc ggggcgttcg
      121 gcggcgtggt tggggttgtt ggcttcggca ggtttgccgt tgagcaaaac cgctttgctg
      181 ttcacaaagc cgcgcgcttc tttattggag gatgccaaac cggtttttac caaggcttcg
      241 acgacattga tgccgtctga aacttcaaat gcaggcaggc cgtcgagggc gagctgctcg
      301 aagtcgcttt cggtcaggct gctttggtct tcggcaaaca ggctttcgga aatgcgttgc
      361 gcggcggcaa gggcttcttc gccgtgaatc aggcgggtca tttcttcggc gaggatgcgt
      421 tgcgcttcgg gcttgctgcc gcttgccttg tctttggctt cgatggcatc gatttcttcg
      481 atggacagga aggtaaagta tttcaggaat ttatacacat cggcatcggc gactttcagc
      541 cagaattggt agaactgata gggcgaggtt tttttcgcgt tcagccatac cgcgccgcct
      601 tcggttttgc cgaatttggt accgtctgat ttggttacca aaggcagggt cagaccgaat
      661 acttgttttt ggtgcaggcg gcgggtcagg tcgataccgg cggtgatatt gccccattgg
      721 tcggagccgc cgatttccaa aaccgcgccg tggcgtttgt tcaactcggc gaagtcgtaa
      781 ccttgcagca gggaataggc gaactcggtg aaggaaatgc ctgcgccgtc gcggtcgatg
      841 cgctgtttga cggattcttt gttcagcatg gcgttgacgg agaaatgctt gccgatgtcg
      901 cgcaggaagt caaggcagtt catgctgccg aaccagtcgg cattgttcgc cataatggcg
      961 gcatttccgc cttcaaagct caagaaaggg gttaattggt tgcggatact ttccacccag
     1021 ccggcaacag tttcggcgga attcaagctg cgttcggcgg ctttgaagct ggggtcgccg
     1081 atcataccgg tcgcgccgcc caccaaagca atcggcgtat gccccgcctg ttggaagcgg
     1141 cgcaatgcca atacgggcag caggtgtccg atgtgcaggc tgtcggcggt cgggtcgaag
     1201 ccgcaataaa gggcaatttt ttgttcgttc aacaaagcgt ctaaggcttc gatgtcggtg
     1261 gtttgcgcga taaggccgcg cgattgcagg tcttggatga cgctcatcgg tctctttcaa
     1321 aaaaaattag cgtttttgca aaccgccgat tgtaacaaat ttaagcgaat caatggttat
     1381 ggcgcgtatc gagaaaccgt tgtttttcgg aaaaacgctt tgccaattcc gtgccgccgt
     1441 aagggttgat gtggtctttg tccgagtaaa ccggcaatcc gccgatttga aaatctgcgg
     1501 ggatataggc ggcggcatca ataatataga cgttggggta tttggctgcc aattccctga
     1561 tgcgtgcatt ggctttcagg gtgctttcgt cgtccgggcg cagggcttgg cggtaacccg
     1621 gtatgcgtga agacaagata taggcgcgct ggacgttgta agacgaggca aggttgtccg
     1681 ccatcaggta aacggcttgt ttttcggacg agagtttatg cagcatacgg tcgaattttt
     1741 ggaaaaaacc ggcatcatag gcaagggagc ggctgttttc gggcatttgg ctgccccagc
     1801 gcatcgccaa aaccactttt gaataccggg gcaggtgttc ttcggcatag cgataaacgg
     1861 cgcggcaggc tgcccagttt tggaacacac gggacgcgta gccttccaca taggcgcaag
     1921 cgtcggcgga aaccatagtg gcggaccatt tttctttttt gcccacggca tcgaagaatg
     1981 ttttgtaatg gtcggcgtgg gagtcgccca aaaccagcag ttccggctgt ttttccgtat
     2041 cccccccata ggcattgttt gccggtattg ttgtggcagg aggtgttgga acgcgtcagc
     2101 cccaagcggt cgtattgcgc cataaacggc agtctcatcg caaaaaacga gcccgccccc
     2161 aaaatgagca taggcaaggc ataaatccat aaaacggatt gtgcgaacga accttgccat
     2221 tttttaaacg gtttttcgat gcagtggtaa gaaaacaggg aaagcagcaa tatcaggacg
     2281 accgccgccg ccggcgaata aggcggcagg ttgtccgggc cgatatagcg cataaaggcc
     2341 aatatcggcc aatgccacag ataaagcgaa taggaaatca aaccggcggc aacagtgatt
     2401 ttcgattgga aaaatttttt aagcgggtgt tcgtaatgat tgaaataaat cagcgcggca
     2461 acagccagac agggaatcaa agcggcgggg cccgggaaat aggcggtttg ttccgaatag
     2521 gaaaacaggc aggttgacaa tatgcacacg gcaaacaatg cgccgacggc ggcacagcgt
     2581 ctgccgacgg caggttgccg gcagcgcatc cacacggcgg tcagcgatcc tatcagtaat
     2641 tcgcaggcgc gcaggtgggg caggtaatat ttatcgagcg cggaaggtat aaaggaggcg
     2701 gcaaggctta aggcacacag tgcggcaagg aagccgaact gtacgcgcag gcttttgcgg
     2761 gcgacaagca gcagcagtat cggaaagaca aagtaaaatt gttcttcgac cgacaaagac
     2821 cagatgtgca gcaggggctt ttcttcctgc gcgggatcga aataatcctt cccccttgca
     2881 aaatacaggt tagaggcgaa acccaaggcg gtcagcgcgg atttccacaa aagaaagaaa
     2941 tcatctttgg tgaataaaaa gaagccgcct gccagcgttg ccgccaatac ggcgaaaaat
     3001 gcgggcagaa tccgcttgat gcggcggata taaaatgcct tcagggaaaa cctccccccc
     3061 ccgacatttc gcggtgaaga atcgtcgtca tcaaaaagcc tgaaatcaca aagaatatat
     3121 cgacaccgag aaacccgccc ggcagccaat ccttttcgat atggaacacg atgacggaca
     3181 agacggcggc ggcgcgcaat gtgtcgatgt ccgggcggta gggtaaggct tggctcataa
     3241 tgtttttata gtggattaac aaaaaccagt acggcgttgc ctcgccttgc cgtactatct
     3301 atactgtctg cggtttcgtc gccttgtcct gatttaaagt taatccacta tactcgaaac
     3361 gcggcggcgc aaatgccgtc tgaaaggtca tttcgtatcg gggatcggga tattcggaat
     3421 gccggacggc ttcccgtaac ggcggggcag gcggtttgtt ttgcaggaat cggggagggc
     3481 aaatcggaaa tgcgggtggg agtttatttt gatgcggctg cattccggcg gtacgggaaa
     3541 cgccgaaaat catcaaaatc ggcttcagac ggcatttccg gcaagccgcc tgaaacctgc
     3601 cgcatttggg ttacacgtta aacaaaaagt gcatcacatc gccgtcttgc acgacatatt
     3661 ccttgccttc cacacgcatt ttgccggctt ctttggcttt ggcttcgccg ccgagcgaga
     3721 caaagtcgtc gtaagaaatg acttgggcgc ggatgaagcc gcgttcaaaa tccgtatgaa
     3781 tcacgccggc ggcttgcggc gcggtgtcgc ctttgtgtat cgtccacgcg cggacttctt
     3841 tcacaccggc ggtgaaatag gtttgcagcc ccaagaggtc gtaaccggca cgaatcaggc
     3901 ggttcaggcc cggttcttcc aagcccattt cggcgaggaa ctcggctttt tcgtcgtctt
     3961 ccaattcggc aatttcgctc tccatcgcgg cgcaaacggc gacgacgggg gcgttttctt
     4021 ttgccgccaa ttctttcagg cggtcgaggt gcggattgtt ttcaaaaccg tcttcggcga
     4081 cgttgcccac atacatcgcc ggtttggcgg tcagcaggaa cagcggtttg agcatcgcgc
     4141 gttcttccgc gtccaaaccg aaggaacgca cgggtttgcc ttcgtccaga tgcggcagca
     4201 gttttttgca caaatcgacc agcttttgcg cgtctttgtc gcctgagcgg gcgcgttttt
     4261 cttcgcggac gatggctttt tcgacacttg ccaggtcggc aagtgccaac tctgtgccga
     4321 tggtttcaat gtcggcaatc ggatcgacgc ggcctgcaac gtggacgatg ttgtcgtcgt
     4381 caaagcagcg cacgacattc acaatcgcat cggtttcgcg gatgttggca aggaactggt
     4441 tgcccaagcc ctcgcctttg ctcgcgcctg caaccaaacc ggcaatatcg acaaattcga
     4501 cgatggcagg ctgcattttt tgcggattga cgatttttgc caattcggcc atacgcggat
     4561 cggggacttc gacgatgccg acgttgggtt cgatggtaca gaaaggatag tttgccgctt
     4621 cgatacccga ttgggtcagc gcgttaaaaa gggtggattt gccgacgttg ggcaaaccga
     4681 cgatgccgca tttcaaactc atgttttttc ctgaaaatag agaaatttaa cggcggatta
     4741 tagcataccg ccgcccgcgt tccgaaaaaa tgccgtctga aacggcttca gacggcatcc
     4801 ggtttcagaa aaccgttcag aacaagccgt gaatcacgcc ttctgcgtcc acatcgattt
     4861 tctcggcagc cggaactttg ggcaggccgg gcattttcat catgttgccg cacagggcga
     4921 cgatgaaacc tgcgcctgcg gaaacggtga tgccgcgcac ggcgatgcgg aagtcttcgg
     4981 ggcagcccaa cagtttggcg ttgtcgctca aagagtattg ggttttcgcc atgcagatcg
     5041 gcattttgtc caagcccagt ttttccagtg aagcgatttc ggcagacgct tccgcgctga
     5101 aatcaacatc ttccgcgccg tacacttttt gggcaatcgc acggattttg tctttgatgc
     5161 ccaactcgac atcgtaggcg aaaccgaagt tattggtttg actttcaatg gcgttgacga
     5221 ctttgcgcgc caaatccgcg ccgcccgcac cacctttgcc ccacacttcg gtcagggaaa
     5281 cttcaacgcc gtgttcggca caggcttttt caatcatcgc caactcggca tcggcgtcgg
     5341 acacgaagcg gttgagcgca acgacgacgg gcagtccgaa tacgtttttc aggttggaaa
     5401 tgtgtttcag caggttgggc aaaccttttt ccaaagcgtc taaattttct tcgccgaggt
     5461 tggcgcgttc cacgccgccg ttatatttca acgcgcggac agtcgccacg acaacagccg
     5521 catcaggttt caaaccggca aggcggcatt tgatgtcgca gaatttttcc gcgcccaagt
     5581 ccgcgccgaa gcctgcttcg gttacggcgt aatcggcaag gtgtttcgcc agacgggttg
     5641 cggttacgga gttgcagccg tgggcgatgt tggcgaacgg gccgccgtgt acgaaggcgg
     5701 gcgtgccttc gatggtttgc accaagttgg gcttaatcgc atctttaagc aatgccgcca
     5761 tcgcgccatt cgctttcaaa tctttggcgt aaacggggct gccgtctttg gcgtaggcga
     5821 caaggatgtt gcccaaacgc tctttcaaat cgctgatgtc tttggcaaga cagaataccg
     5881 ccatcacttc ggaagcaacg gtaatatcga aaccgtcagg acgcatcacg ccgtcaacgg
     5941 gtttacccat gccgtcgatg atgttgcgca actggcggtc gttcatatcg accacgcgcc
     6001 gccacagcac gcgtttgggg tcgatgttca actcgttgcc ttggtagata tggttgtcga
     6061 gcatcgcggc aagcagatta tttgccgcac cgatggcgtg aaaatctccg gtgaagtgca
     6121 ggttgatgtc ttccatcggc aaaacttggg catagccgcc gcctgccgcg ccgcctttca
     6181 cgccgaacac cggccccaga gaaggttcgc gcagggcaat cacggcatct ttgccgatgt
     6241 ggcgcaacgc gtccgccaaa ccgatggtta cggtggtttt gccttcgccc gccggagtcg
     6301 ggttgatggc ggtaaccaaa atcagcctgc cctgtttttg cggcagtttg aacgcttcgg
     6361 caggattgat tttcgccttg taatgaccgt aaggctcaat gttgtcggca ttcagaccaa
     6421 gcttggcggc aatttcgcca atcgggcgca tggtggagga ttgggcgatt tcggcatcgg
     6481 ttttgaagct catgattttc ctttagaaat gaggagggac atgccgtctg aaagcatcag
     6541 gcgacaaaca ggtggattga aaataatatc aggcatatta taacgttatc cgcaccaaac
     6601 ccgcagtgaa atttttgacg cagcaacaaa aataccgttc atattgttca caatccaagg
     6661 agaaaacatg ggcagcaacg catggctgtt ttgggcattg gcatcggcag gcttcgcctc
     6721 attgaccgct attttcgcca aaatgggttt acagggtata gattccgatt tcgccacctt
     6781 tatccgcacc ttggtcatcc ttgccgcttt gttattgttt ttaacctaca ccggcaaatg
     6841 gcagggtgtg aacggcttta cggggcgcaa ctggacattc ctcatcctat ccggtcttgc
     6901 taccggcgca tcttggctcg cctattttaa agccctgcaa ctgggcaacg cctcgcaagt
     6961 cgcccccatc gacaaattca gcctggtctt ggtcgcgctg atggcggtgg ttttcttgga
     7021 cgaacgcccg aacacgcagg aatggatagg cttggggctg gtaacggcgg gcgtgttggt
     7081 gctggcgttg aaacgttaaa ccgaatccgc cataccgtct gaaaccgggt ttttacttcc
     7141 aagcccctgc aagggcttga gcctctttca gacggcatac cgtgccgaca tccagccaca
     7201 agcccgtatg cttctgaccg ctcacgcggt tttgccgcat ttcgccacgc aatacgggcg
     7261 cgagtttcgc cacactgccc gcttcgattc cgtcaaacat ttcaggacgg taaataccca
     7321 cgccgctgaa tgtcaatccg ttgccgccat ttacttccgg ccgcacgctg ctgtcgggca
     7381 gcagggaaaa atcgccgtcg gggttgtgcg gcggattttc caccagccac agatgggcgg
     7441 aaatatgttc cggcagggac gatgccgtct gaaacgcggc ggtaaaatcg atgtcggtca
     7501 gcacgtcgcc gttgaccacc aaaaacggct gcccacccaa cagcggcaat gcctgcgcga
     7561 tgccgcctgc cgtttccaaa ccgcctgcgg gttcgggcga ataggcgatg ttcacgccat
     7621 aagccgagcc gtcgcccaaa gcatcttcta tctgccgacc cagccaagcg tggttgatga
     7681 cgatttcggt aaaccccgcc tgcttcagac ggcataggtg ccaaccgatt agaggcttac
     7741 ccgccacatc gagcagcggc ttcggagtgg tatcggtcaa agggcgcata cgctcgccgc
     7801 gtcctgccgc cagtatcatc gctttcatat atctgtccga atatcagtct aaaaatctaa
     7861 actgccgtct gaaatacagc agcgcggggc gtttgcaccc gcagtttttg atttcgtcga
     7921 gcctgacgta aaacacaaaa tgcgtgccga tttcatgttt gccgacaata tgcccgtgca
     7981 ggtgcgccaa cgcgccctct atttcaagtt gtcccgtttt gccgcgatgc cagatgtggt
     8041 aggcaaaccg ctcttcgggc gacaggccgg tcagcccggc aaaatgttcg gcaacatcct
     8101 gatgttcgtc cgccagcgta ttgatgcaga ggctgccgtt ttccgacagg atcggaatga
     8161 ttcgcgcact ccggttgatg cacagcatca cggtcggcgg ctcgtcggta accggcgcga
     8221 ccgccgtcat tgtaatgccg taacgccctg ccgcaccgtc tgtcgtgatg acatgaacgc
     8281 ctgccgcgca agatgccatc gcatcacgga acgaagtttg aaaatttttc tgcaaatccg
     8341 ccatttttcc cctttaaact gtcccctata taagaatgct gcacacaagg catcccccat
     8401 gtgcagcagt tttgattcaa aaagccgtcg gtcggacgtt tccgcgcgtt acggcgtatt
     8461 acgagttcaa cgcatcctcg attttggcaa gttctgccaa caggtcttta agcagcagca
     8521 ttttctcgcg gcccagcact tcctcgatag cgtcgtagcg ttcgtccact tcttcgccga
     8581 tttcctcata cagcttctcg ccctcggcag tcagcttcag aaaaacacgt cgttggtcgt
     8641 tggaaggttt caggcggaca accaaacccg ctttttcaag gcgggtcagg ataccggtca
     8701 ggctggggcg caaaatgcac gcctgattcg ccaaatcttg aaagtccagc gtgccgtttt
     8761 ccgccaaaag acggataatc cgccattgct gatcggtaat attcgcctga ttcagaatag
     8821 gcctgaattg ggtcatcagg gcttcccttg cctgtatcag accgatattg atagacgcat
     8881 gttttgattg ggtaggcatt gtttaagtct ccaagttatc gaaaatcaaa ctttcaaacc
     8941 gtcgggaaag cctgtgggcg taaattttga tgcaaccgtt atataacaaa acgaacatat
     9001 agcaacaata cgctataaac cgcatcggac gactgggtat aaaagacttt aattccgata
     9061 atcctatcta aaaatatttt aatagttata tcttaatcta tttttcccac aatcacaaca
     9121 agggattaca tcggcaggcg cgtcggctct ttcccaaaaa acaaaagccg ccgcatccgc
     9181 cgcgcaaggc atatgccgct tgattctcta catagcggaa aatttaataa aaacaaaagt
     9241 taaccgaaaa catccgcctg aaaaattcgt gcgcgcaagc cccaataact gctgattccc
     9301 gtcgtatagt gaaccatttt cccatttttg accaaaacga cggcaggcgt tgcgacaatc
     9361 cgccaagacc ttgccaaacc cccgtcctca tcgttgacag tcggaaagcc caagccgcgt
     9421 tttgccatat acgccgccac ttccgccgaa ctgccggaac gtaccgccac gccgacgacc
     9481 ggcacgccgt ccgccgccaa atcatcgatt atcggcgact gataacggca cacgccgcac
     9541 cagctccccc aaaaatacac caaaaccgcc ttatctcggc taaactgtcc caaagtcagc
     9601 cgctgccccg acagcagggt caaaggccgc cctgccgcac cggccggctc ttcgggcttg
     9661 cgtatccaat ccaaaaacag cgacaccaat aaaaacacca atgccgtctg aacggcaaat
     9721 ttgatgcccg aaagcagttt ctttttcata cgctctctca aacggtacgc ccgcgcaacc
     9781 ggcaggcaaa caaaaagcca agtctcaaaa cttggcttcc ggttatctgg tgggtcgtga
     9841 gcgattcgaa cgctcgacca acggattaaa agtccgctgc tctaccgact gagctaacga
     9901 cccgataagc cgtgcattat acagcaccat cctacctcgt caagcaaatt ttacaggctt
     9961 aattgcagac cactgtttgc acgggatatt ttgacaacgg attttcacaa tccgccgcat
    10021 accgtgtaaa agttcgcaca aggaaaagca aaccgcccga aatcaatgta cactttccgc
    10081 ccgtttccct tcccaacctg cacacagaaa cacacattat gaacatacaa aacatccgca
    10141 ccctcctcga caccgtcgcc gttccgaata cggcacgcac gctcggcggc gaaaaggccg
    10201 tccgttcggt cgaacagcgt tcagacggca tccatatcgc cctgcatttc ggcttccccg
    10261 tcgcgcacat tgcctcagaa acagccgacc gcatacagga aatcctgatg cccgaaacag
    10321 gcgacacaca catccatctg tccatggaca ctgaaatcgg cacacacaaa gtccagcccg
    10381 gcgttaccac catcaaaggc gtgaaaaaca tcatcgccgt cgcatcggga aaaggcggcg
    10441 tgggcaaatc gacaaccacc gccaaccttg ccgccgcaat ggcgcgcatg ggcgcgcgcg
    10501 tcggcgtgct cgatgccgac ctttacggcc cgagccaacc gaccatgttg ggtgtggacg
    10561 accgcaaacc cgatcagaaa aaccaaaaac tcattcccgt cgaatcttca gacggcatac
    10621 aggtcatgtc tatcggcttt ctcgtcgata ccgaccaagc cgtcgtctgg cgcgggccga
    10681 tggtcagcca agccttgcag cagctgatgt tccaaagcga gtgggacgaa gtggactacc
    10741 tgtttatcga cctgcccccc ggcacgggcg acatccaact cacgctgtcc caacgcatcc
    10801 ccgtaaccgg atcggtcatc gtaaccacgc cgcaggacat cgccctgata gacgcgcgca
    10861 aagccgtgga tatgttccgc aaagtcaaca ttcccatttt gggcgtattg gaaaatatgt
    10921 ccgtccacat ctgctccaac tgcggacaca gcgaagcact gttcggcacg gacggcggca
    10981 aagatttggc ggcacgcctc aacgtccccc tgctcggaca gcttccccta agcctgcccg
    11041 tgcgcgaagc catggacggc ggcacaccag cgcaactgtt cgacgaacac cccgccatcg
    11101 ccaaaatcta caccgatgcc gcattccaaa tcgccctgag cattgccgac aaaggcaaag
    11161 acttcagcag ccgcttccct aaaattgtcg tcgaataaag ccgcgtccga aaccgcaaca
    11221 gcaatgccgt cccaagcccc gcgcctgccg gcgggcaaac ttgccggata aaacggtttt
    11281 tttgagattt tacgttccgg attcccgcct gcgcgggaat gacgaatttt aggtttctga
    11341 ttttggtttt ctgttttgta ggaatgatga aattttgagt tttaggaatt tattggaaaa
    11401 aacagaaacc gctccgc
//
LOCUS       DAWGRZ010000048        11403 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000048 DAWGRZ010000000
VERSION     DAWGRZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11403
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.048"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..79)
                     /locus_tag="WHT44_000921"
     CDS             complement(<1..79)
                     /locus_tag="WHT44_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ2533124.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="WHT44_000922"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="WHT44_000922"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ2533125.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMVGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="WHT44_000923"
     CDS             complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="WHT44_000923"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ2533126.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRTFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKESAAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGEKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(3693..4598)
                     /gene="xerC"
                     /locus_tag="WHT44_000924"
     CDS             complement(3693..4598)
                     /gene="xerC"
                     /locus_tag="WHT44_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ2533127.1"
                     /translation="MGLDGLAAYLDAYLENIVREGKSEHTVAAYRRDLQELLALLEEM
                     PSANPSNCTRGDFVQALRRLSGRGLGERTLARKLSSWRQYCVWLVKRGLMHADPTADI
                     KPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIHGLNA
                     DDVYLDEAWVHVIGKGRKQRQVPLVGKSVEALKNYLPLRQTASDGKALFTGRNGTRLS
                     QRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLSTTQI
                     YTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4782..5846
                     /locus_tag="WHT44_000925"
     CDS             4782..5846
                     /locus_tag="WHT44_000925"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ2533128.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVDAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVCFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            5866..6828
                     /locus_tag="WHT44_000926"
     CDS             5866..6828
                     /locus_tag="WHT44_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H-binding protein"
                     /protein_id="HEZ2533129.1"
                     /translation="MPSEPPFGRHLIFASLTCLIDAVCKKRYHNQNVYILSILRMTRS
                     KPVNRTAFCCLSLTTALILTACSSGGGGVAADIGAGLADALTAPLDHKDKGLQSLTLD
                     QSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLESGE
                     FQVYKQSHSALTAFQTEQIQDSEHSGKMVAKRQFRIGDIAGEHTSFDKLPEGGRATYR
                     GTAFGSDDAGGKLTYTIDFAAKQGNGKIEHLKSPELNVDLAAADIKPDGKRHAVISGS
                     VLYNQAEKGSYSLGIFGGKAQEVAGSAEVKTVNGIRHIGLAAKQ"
     gene            6975..7652
                     /gene="tsaB"
                     /locus_tag="WHT44_000927"
     CDS             6975..7652
                     /gene="tsaB"
                     /locus_tag="WHT44_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ2533130.1"
                     /translation="MQADFNRPVLAVDTGTSRLSLALRADGETRLFHQEVGSRQSELI
                     LPEIRTLFRDAGITAADLGAVVYAQGPGAFTGLRIGIGVAQGLATPFDTPLIGVPSLD
                     AAASLPPPQSCILAATDARMGEVFYAWFDTLNCHRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEEAPPFSGRPDMPTAADFLALAAKGGYPAVHAAHAGLLYVRNKIALTAKEQ
                     AERRARP"
     gene            7649..8089
                     /gene="rimI"
                     /locus_tag="WHT44_000928"
     CDS             7649..8089
                     /gene="rimI"
                     /locus_tag="WHT44_000928"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ2533131.1"
                     /translation="MNIRRAVCADCEELAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRQGIASALLEYWFTHLPEDTQRLLLE
                     VRAGNTAAQALYTAHGFSITGRRKNYYRTADGKTEDAVLMEKIC"
     gene            8083..8820
                     /locus_tag="WHT44_000929"
     CDS             8083..8820
                     /locus_tag="WHT44_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ2533132.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPAPSAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVPTRKPAPETETPLPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGILLDNILKAVGLDAAYVHKTCWVKTAAVGNPMP
                     SEQAVANALGQIARELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARAWQVLKQLKRALRQGGGS"
     gene            8882..9523
                     /gene="pyrE"
                     /locus_tag="WHT44_000930"
     CDS             8882..9523
                     /gene="pyrE"
                     /locus_tag="WHT44_000930"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ2533133.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9597..10031
                     /locus_tag="WHT44_000931"
     CDS             9597..10031
                     /locus_tag="WHT44_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533134.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10028..11338
                     /gene="argA"
                     /locus_tag="WHT44_000932"
     CDS             10028..11338
                     /gene="argA"
                     /locus_tag="WHT44_000932"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ2533135.1"
                     /translation="MSAPDLFVAHFREAVPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFYLDMLQTAASAAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
BASE COUNT         2551 a         3573 c         2995 g         2284 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccgaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccatacagc cggtttcata cacatctccc attaaagcca aacattatac agccgtcccg
      121 accgattaaa ttcatatttt aaaacaatat cctgcctcca aaacccacat cgtgctataa
      181 tccgcaccga ttttcagacg gcatcgtcgt gccgtctgaa attttttcat tccaacaaca
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gcaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgcgaa aaagcgcaag aaaaagtctt ctccgatttg gggcgcgtgc
      481 gtccgctcaa agaaaaaaac cccggcctca tcatcggcgt tgccggctgc gtcgcctcgc
      541 aagaaggcga aaacatcatc aaacgcgcgc cttatgtgga cgtggttttc ggcccgcaaa
      601 cgctgcaccg cctgccaaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg
      661 atatttcctt ccccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg
      721 gcgcggcatt tgtatcgatt atggaaggct gttccaaata ctgctccttc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cgctcaacga cgtattgacc gaaatcgcca
      841 accttgccca gcaaggcgtg aaagaaatca acctcttggg acaaaacgtc aacgcctatc
      901 gcggcgaaat ggacgacggc gaaatctgcg acttcgccac cctgctgcgc atcgtccacg
      961 aaatccccgg catcgaacgt atgcgcttca ccaccagcca cccgcgcgag tttaccgact
     1021 cgattatcga gtgctaccgc gacctgccca aactggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcaa tgaaacgcgg ctacaccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgcgccatcc gtcctgattt gtgcctgagc agcgatttca
     1201 tcgtcggctt ccccggcgag accgaacgcg agttcgagca aaccttgaaa ctggtgaaag
     1261 acatcgcctt cgacttgagc ttcgtgttta tttacagtcc gcgccccggc acgcctgccg
     1321 ccaacctgcc ggacgacacg ccgcacgaag aaaaagtgcg ccgcctcgaa gccttgaacg
     1381 aagtcatcga agccgaaacc gcgcgcatca accaaaccat ggtcggcacg gtacaacgct
     1441 gcctggtcga aggcatctcc aaaaaagacc ccgaccaact gcaagcccgt accgccaaca
     1501 accgcgtcgt caacttcacc ggcacgcccg acatgattaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacaccttc tccctgcgcg gcaaagttgt cgaagcctaa accctcacgc
     1621 cgaaaaaatg ccgtctgaag cgtttcagac ggcattttgc cttgtatcgg cagacgacgg
     1681 cgcggccggg cggcttaatt tgccgcatcc cgatccgaca gccacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa gcccaaatcg tctaaaagtt ttttcggatc gccgtgtccg
     1801 gttacggtat cggcaacgcc caaaagcaaa acgggtttgc agatgccgtg tttcgccaat
     1861 acttccagca ccgcgccgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg
     1921 cggtcgtggc ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacgaagcgc
     1981 atatcggcga cggtggcgtt cagtttttcg gcaaccgcca atgcgggggc gaccatactg
     2041 ccgaaggcaa tgaatgcggt tttctcacct tcgcggcgga taatgccctt gccgatttcc
     2101 acggtttcca tgccgtctga aaccggcgcg cccgtacccg tgccgcgcgg atagcggacg
     2161 gcggcgggcg cgtctgcctg atagcaggtc gaaagcaaca ggcggcattc gttttcatcg
     2221 ctcggcgcgg cgacaatcat gttcggcacg cagcgcaaaa agctcaaatc gtacagaccg
     2281 gcatgggtcg ggccgtccgc gccgacgatg cccgcgcggt cgacggcaaa caaaacgggt
     2341 aggttttgca gggcgatgtc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa
     2401 taaatcgcca cgacgggctt catcccttcg caagccaaac cgccggcaaa ggtaacggcg
     2461 tgctgctcgg cgatgccgac atcgaaatag cggtcgggga atcgttgttc aaactcaacc
     2521 aagccgctgc cctcgcgcat ggcgggggta atcgcaacca gtcgggaatc tgccgccgcc
     2581 cggtcgcaca gccatttgcc gaacacttgg gtataggtcg gtttggcggc gggcttgggt
     2641 tctttttcag acggcatttg cgccgcgctt tctttaggca ggttggcgac ggcgtggtat
     2701 ttgacggggt cgttttcggc gagtttgtag ccgttgccct ttttggtgat gacgtgcagc
     2761 aactgagggc ctttgcggct gcgcaagtct ttcaatacgt ccaccagatt ttcgacgttg
     2821 tgtccgtcca cggggccggt gtagcggaag ccgaagtttt caaacaaaga cagcgactgt
     2881 ttggcgtgtt cggcttcttc ggcaagggtt ttgattttgt gttcgacttt ttgggcaaac
     2941 tccatcgcgc cgggtatttt gtctaatacc ttgcccgttt gcgctttgac ggtactcaac
     3001 aggccgtgca tatcgcgcac gacgttgctg gcaaggtatt tcggcagcgc gccgacgttg
     3061 ggggaaatcg acatttcgtt gtcgttgagg acgaccagca aatccacatc catatcgcct
     3121 gcgcaattca aggcttcaaa cgcctgcccc gccgtcatcg cgccgtcgcc gatgatggcg
     3181 acgctgcggc ggtcgctgcc caagagtttg tctgccgccg ccatgcccaa cgccgcgccg
     3241 atggaggtgg aggaatgccc cacgccgaac gcgtcgtact cggactcgca acgtttcgga
     3301 aaacccgcca aaccgccata ttggcgcatg gtgtgcatct ggtttttcct gcctgtcagg
     3361 attttgtgcg gatagctttg gtgtccgaca tcccacacca gcttgtcttc gggcgtgtcg
     3421 tacacatagt gcagggcgat ggtcagttcg accgcgccca aattgctggc gaaatgcccg
     3481 ccggtctgcc cgacagattc cagcagaaag gtgcgcaact cgccggcaag gcgcggcagc
     3541 tgttttttgt ccagacggcg caaatcttgc gggctgtcaa tcaggtcgag tagggggctt
     3601 gggttcatgg tgtgtctttt ttatgtgtcg tccgggtgca acggtcaatt atatatcaag
     3661 agcgtgcggc tgacggctga ttttgccgta tgtcattcgt cctgccgctt ggcgcgcggg
     3721 tgggcttcgt catacaggcg ggcgatgtgg tcgaaatcga gcttggtata aatctgcgtg
     3781 gtcgaaaggc tgctgtgccc gagcagctcc tgcaccgccc tgatgtcgcg cgaagcctgc
     3841 aataggtgtc cggcgtagct gtggcgcatc atatgcggcg aaacgtgcct gccgtcgccg
     3901 ttttgcgccg cccattgcgc caaacgtttt tggatttggc gttggctcag gcgcgtgccg
     3961 ttcctgccgg taaacagggc tttgccgtcc gatgccgtct gacgcagcgg cagatagttt
     4021 ttcagggctt ccacgctttt gccgaccagc ggcacctgcc gctgcttgcg ccctttgccg
     4081 ataacgtgta cccacgcctc gtccaaatag acatcatctg cattcaagcc gtgtatctcg
     4141 ctcacgcgca aaccgctgcc gtacatcagt tcgaacaggg cgtggtcgcg caccgccagc
     4201 gggtcgccgc cgtccacggg caaatccagc atccggttca gccattcctg cggcagggct
     4261 ttgggtacgc gctcgggctg cttcggcggt ttgatgtcgg cggtcgggtc ggcgtgcatc
     4321 aggccgcgct ttaccagcca aacgcaatac tgccgccaag acgaaagctt gcgagccagc
     4381 gtccgttccc ccaaaccgcg gccggacagc cggcgtaatg cctgtacgaa gtcgccgcga
     4441 gtgcaatttg aagggtttgc agacggcatt tcttccagaa gggcaagcag ttcctgcaag
     4501 tcgcgccggt atgcggcaac cgtgtgctcc gatttaccct cgcgcacgat attttccaaa
     4561 taagcgtcca agtatgccgc aagtccgtcc aaacccattc ccacacctaa aataacatta
     4621 gaaacattat cataaatcgg aatatccgaa tcccgaaacg tcaaaacccg acaaacctgc
     4681 atactggcat cgttaatata aaatcaatga gctgtttatg gttttttgct gtaaaaaaca
     4741 ttataatccg ccttatttac ctattgccca aggagacaca aatggcactc gtatccatgc
     4801 gccaactgct tgatcatgct gccgaaaaca gctacggcct gccggcgttc aacgtcaaca
     4861 acctcgaaca gatgcgcgcc atcatggagg ctgcagacca agtcgacgcc cccgtcatcg
     4921 tacaggcgag tgccggtgcg cgcaaatatg cgggtgcgcc gtttttacgc cacctgattt
     4981 tggcggctgt cgaagaattt ccacacatcc ccgtcgtcat gcaccaagac cacggcgcat
     5041 cacccgacgt gtgccaacgc tccatccaac tgggcttctc ctctgtaatg atggacggct
     5101 cgctgatgga agacggcaaa accccttctt cttacgaata caacgtcaac gccacacgta
     5161 ccgtggttaa cttctcccac gcttgcggcg tatccgttga aggcgaaatc ggcgtattgg
     5221 gcaacctcga aaccggcgaa gcaggcgaag aagacggtgt aggcgcagtg ggcaaacttt
     5281 cccacgacca aatgctgacc agcgtcgaag atgccgtatg tttcgttaaa gataccggcg
     5341 ttgacgcatt ggctattgcc gtcggcacca gccacggcgc atacaaattc acccgtccgc
     5401 ccacaggcga tgtattacgt atcgaccgca tcaaagaaat ccaccaagcc ctgcccaata
     5461 cacacatcgt gatgcacggc tccagctccg ttccgcaaga atggctgaaa gtcatcaacg
     5521 aatacggcgg caatatcggc gaaacctacg gcgtgccggt tgaagaaatc gtcgaaggca
     5581 tcaaacacgg cgtgcgcaaa gtcaacatcg ataccgactt gcgccttgct tctaccggcg
     5641 cggtacgccg ctaccttgcc gaaaatccgt ccgactttga cccgcgcaaa tacctgagca
     5701 aaaccattga ggccatgaag caaatctgcc tcgaccgtta tcttgcgttt ggctgcgaag
     5761 gtcaggcagg caaaatcaaa cctgtttcgt tggaaaaaat ggcaagccgt tatgccaagg
     5821 gcgaattgaa ccaaatcgtc aaataacagg ttgcctgtaa acaaaatgcc gtctgaaccg
     5881 ccgttcggac gacatttgat ttttgcttct ttgacctgcc tcattgatgc ggtatgcaaa
     5941 aaaagatacc ataaccaaaa tgtttatata ttatctattc tgcgtatgac taggagtaaa
     6001 cctgtgaatc gaactgcctt ctgctgcctt tctctgacca ctgccctgat tctgaccgcc
     6061 tgcagcagcg gagggggtgg tgtcgccgcc gacatcggtg cggggcttgc cgatgcacta
     6121 accgcaccgc tcgaccataa agacaaaggt ttgcagtctt tgacgctgga tcagtccgtc
     6181 aggaaaaacg agaaactgaa gctggcggca caaggtgcgg aaaaaactta tggaaacggt
     6241 gacagcctca atacgggcaa attgaagaac gacaaggtca gccgtttcga ctttatccgc
     6301 caaatcgaag tggacgggca gctcattacc ttggagagtg gagagttcca agtatacaaa
     6361 caaagccatt ccgccttaac cgcctttcag accgagcaaa tacaagattc ggagcattcc
     6421 gggaagatgg ttgcgaaacg ccagttcaga atcggcgaca tagcgggcga acatacatct
     6481 tttgacaagc ttcccgaagg cggcagggcg acatatcgcg ggacggcgtt cggttcagac
     6541 gatgccggcg gaaaactgac ctacaccata gatttcgccg ccaagcaggg aaacggcaaa
     6601 atcgaacatt tgaaatcgcc agaactcaat gtcgacctgg ccgccgccga tatcaagccg
     6661 gatggaaaac gccatgccgt catcagcggt tccgtccttt acaaccaagc cgagaaaggc
     6721 agttactccc tcggtatctt tggcggaaaa gcccaggaag ttgccggcag cgcggaagtg
     6781 aaaaccgtaa acggcatacg ccatatcggc cttgccgcca agcaataacc attgtgaaaa
     6841 tgccgtccga acacgataat ttaccgttcg gacggcattt tgtattgcac cgtccgacgg
     6901 catgcccaag gggggaaatc cctattttca ggccaaccgc tatataatgc cgtctgaacc
     6961 aacgagagaa tgccatgcaa gctgatttta accgtcccgt cctggccgtc gataccggta
     7021 cttcccgttt gtcgctcgcg ctgcgtgccg acggcgaaac ccgtctgttc catcaggaag
     7081 tcggcagccg ccagtccgaa ctgattctgc cggaaatccg caccctattc cgcgatgcag
     7141 gcattaccgc cgccgatttg ggtgcggtcg tgtacgcaca gggtcccggc gcgtttaccg
     7201 gactgcgtat cggcatcggt gtagctcagg gtttggcaac gccgtttgat acccccttaa
     7261 tcggcgtacc ctcgctcgat gccgccgcct cgctgccgcc gccgcaaagc tgcatccttg
     7321 ccgctacgga cgctcgtatg ggcgaagtgt tttatgcatg gttcgatacg ctgaactgcc
     7381 accgtttgag cgattatcag gtcgggcggg cggcagacat ccggctgccg gagggatgcg
     7441 ccttttcaga cggcataggc agcgcgttcg cgctggaaga agctccgccg ttctcaggca
     7501 gaccggatat gccgactgcc gccgactttc tcgcattggc agccaagggc ggttatcctg
     7561 ccgtccatgc cgcacacgcc ggtttgctct acgtccgcaa caaaatcgcc ctgactgcca
     7621 aagaacaggc cgaacggaga gcgcgcccgt gaacatccgc cgtgccgttt gtgccgattg
     7681 tgaggagctg gccgcactcg atgccgtctg caacccgtcc gcatggacgc aacgccaatt
     7741 tgagtccgca ctggtttcgc cgtccgaaca ggttttcctt gcggaaaaag acggcgggat
     7801 tgccgccttt atcgtttggc agaacctgcc cgacgaatcc gaactgcacc tgattgccac
     7861 cgcgcccgaa tgccgccgcc aaggaattgc gtccgccctg ctcgaatatt ggttcacaca
     7921 tctgcccgaa gacacgcaac gcctgctgct cgaagtccgt gcaggcaaca ccgccgcaca
     7981 ggcactgtac acggcgcacg gcttcagcat tacgggcagg cggaaaaact attaccgtac
     8041 agccgacggt aaaaccgaag atgccgtctt aatggagaaa atatgttaag cgcgcgctac
     8101 ctccacctgc acgaagcttt gggtttgggt ccgatgtggc tgaaacaggc cgccgccgtc
     8161 ctgccgccca aaaacacacc cgcaccctcg gcacaggcac gtccccaaac cgtccgcgcc
     8221 gccccgatcc gcccttccca accccataac ggtcaggcgc ggctcgaaac gatgaaagcg
     8281 ttggaaaccg ccgccgtacc tacgcgcaaa cccgcgcctg aaaccgaaac gcctctgccc
     8341 ggcctttcag acggcatcgc ccccgttccc gccgcttcgg gcatcaccaa gcttgccgtc
     8401 gtcagccttt gcccaccgat cgaggatgcg gtttacgggc aactgttcca cggcaaagca
     8461 ggcatcctgc tcgacaacat actcaaagcc gtaggactgg atgccgccta tgtccacaaa
     8521 acctgttggg tgaaaaccgc cgccgtcggc aacccgatgc cgtctgaaca ggccgtcgcg
     8581 aatgcgctgg gtcaaatcgc ccgcgaactc gacggctgcc gcgccccggc tgtccttttc
     8641 ctcgggcagg cttttgtcca gcctgaacgg caaacgatga ttgaaacttt gtgcggcagc
     8701 cgtcccttct tcatcatcga ccatcccgcc cggcttttac gccaacccga actcaaagcc
     8761 cgcgcctggc aggtgttgaa acagttgaaa cgcgccttgc ggcaaggcgg cggcagttga
     8821 agcgcgccgc acggggcggt agaatcgcaa ctgcgtccca atatctgaca gaaagcacaa
     8881 aatgaccgat ttccgccaag atttcctcaa attctccctc gcccaaaatg ttttgaaatt
     8941 cggcgaattt accaccaagg caggacggcg gtcgccctat ttcttcaatg ccggcctctt
     9001 taacgacggc ttgtccacgc tgcaactggc aaaattttac gcacaatcca tcattgaaag
     9061 cggcatccga ttcgatatgc tgttcggtcc cgcctacaaa ggcattattt tggcggcggc
     9121 aaccgcgatg atgctggcgg aaaaaggcgt gaacgtcccg tttgcctaca accgcaaaga
     9181 agccaaagac cacggcgaag gcggcgtgtt ggtcggcgcg ccgcttaaag ggcgcgtgct
     9241 gattatcgac gacgtgattt ccgccggcac atccgtacgc gaatcgatca aactgattga
     9301 agcggagggt gcaacccccg ccggtgtcgc catcgcgctc gatcgcatgg aaaaaggcac
     9361 gggtgaattg agcgcggttc aggaagtgga aaaacaatac ggtctgcccg tcgcccccat
     9421 cgccagcctg aacgatttgt ttattctgtt gcaaaacaac cccgaattcg gacagttcct
     9481 cgaacccgtc cgagcctacc gtcggcagta cggcgtagaa taaaaacaaa gcatatgccg
     9541 tccgaaccgc cttacgcctc agacggcatc aaacctgaca cacacgagga aataccatgc
     9601 ccgcctgttt ctgcccccac tgcaaaaccc gtctctgggt caaagaaacc caactcaatg
     9661 tcgcccaagg cttcgtcgtc tgccaaaaat gcgaaggact gtttaaagcc aaagaccatc
     9721 tggcaagcac gaaagaaccc atattcaacg atttgcccga ggctgtttcg gatgtcaaac
     9781 tcgttcaccg tatcggcacg cgcgccatcg gcaagaaaca gatttcccgt gacgaaatcg
     9841 ccggcatcct caacggcggt acaacccagc ccgatattcc gcccgcaacc gccgccaccc
     9901 ctgctgccgc accgcaggtt accgtaccgc ccgccgcgcc cgcccgtcag gatgggttca
     9961 actggacgat tgcaaccctg tttgccctta tcgtcctcat tatgcagctt tcctacctcg
    10021 tcatcctatg agcgcgcccg acctctttgt cgcccacttc cgcgaagccg tcccctacat
    10081 ccgccaaatg cgcggcaaaa cgctggtcgc cggcatagac gaccgcctgc tcgaaggtga
    10141 taccttaaac aagctcgccg ccgacatcgg gctgttgtcg caactgggca tcaggctcgt
    10201 cctcatccac ggcgcgcgcc acttcctcga ccgccacgcc gccgctcaag gccgcacgcc
    10261 gcattattgc cggggcttgc gcgttaccga cgaaacctcg ctcgaacagg cgcagcagtt
    10321 tgccggcacc gtccgcagcc gttttgaagc cgcattgtgc ggcagcgttt ccgggttcgc
    10381 gcgcgcgcct tccgtcccgc tcgtatcggg caacttcctg accgcccgtc cgataggtgt
    10441 gattgacgga accgatatgg aatacgcggg cgttatccgc aaaaccgaca ccgccgccct
    10501 ccgtttccaa ctcgacgcgg gcaatatcgt ctggctgccg ccgctcggac attcctacag
    10561 cggcaagacc ttctatctcg atatgcttca aaccgccgcc tccgccgccg tctcgcttca
    10621 ggccgaaaaa ctcgtttacc tgaccctttc agacggcatt tcccgccccg acggcacgct
    10681 cgccgaaacc ctctcggcac aggaagcgca atcgctggcg gaacacgccg gcggcgaaac
    10741 gcgacggctg atttcgtccg ccgttgccgc gctcgaaggc ggcgtgcatc gcgtccaaat
    10801 cctcaacgga gccgccgacg gcagcctgct gcaagaactc ttcacccgca acggcatcgg
    10861 cacgtccatt gccaaagaag ccttcgtctc catccggcag gcgcacagcg gcgacatccc
    10921 gcacatcgcc gccctcatcc gcccgctgga agaacagggc atcctgctgc accgcagccg
    10981 cgaatacctc gaaaaccaca tttccgaatt ttccatcctc gaacacgacg gcaacctgta
    11041 cggttgcgcc gccctgaaaa cctttgccga agccgattgc ggcgaaatcg cctgccttgc
    11101 cgtctcgccg caggcacagg acggcggcta cggcgaacgc ctgcttgccc acattatcga
    11161 taaggcgcgc ggcataggca taagcaggct gttcgcactg tccacaaata ccggcgaatg
    11221 gtttgccgaa cgcggctttc agacggcatc ggaagacgag ttgcccgaaa cgcggcgcaa
    11281 agactaccgc agcaacggac ggaactcgca tattctggta cgtcgcctgc accgctgacc
    11341 gcaacggaaa gccgccgcag aaatgccgtc tgaaccccgt ttcagacggc atttccccga
    11401 tta
//
LOCUS       DAWGRZ010000049        11311 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000049 DAWGRZ010000000
VERSION     DAWGRZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11311
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.049"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            57..338
                     /locus_tag="WHT44_000933"
     CDS             57..338
                     /locus_tag="WHT44_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ2533136.1"
                     /translation="MIHEEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDEATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            complement(359..1585)
                     /locus_tag="WHT44_000934"
     CDS             complement(359..1585)
                     /locus_tag="WHT44_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ2533137.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(1600..2025)
                     /locus_tag="WHT44_000935"
     CDS             complement(1600..2025)
                     /locus_tag="WHT44_000935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533138.1"
                     /translation="MPSEHAAYADLTASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSAGGIAGAAVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            2130..2789
                     /gene="queC"
                     /locus_tag="WHT44_000936"
     CDS             2130..2789
                     /gene="queC"
                     /locus_tag="WHT44_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ2533139.1"
                     /translation="MSNQQALVIFSGGQDSTTCLIQAIQTYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            2817..3335
                     /locus_tag="WHT44_000937"
     CDS             2817..3335
                     /locus_tag="WHT44_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ2533140.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDNYREAVPALKAAWFG
                     DADKIHIDGWQIVEWADGYDIAVSETPKTKMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            3342..3764
                     /gene="queD"
                     /locus_tag="WHT44_000938"
     CDS             3342..3764
                     /gene="queD"
                     /locus_tag="WHT44_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ2533141.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGIIKGG
                     AKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNSRECQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYGRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            4033..4404
                     /locus_tag="WHT44_000939"
     CDS             4033..4404
                     /locus_tag="WHT44_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ2533142.1"
                     /translation="MKLLFILLVLFVAVEHFYIAWLEMTQIPSEKAAEIFKLPYEFME
                     QKQVQTLFSNQGLYNGFLGIGLVWSRFAAPDNAVYGATTLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAMLAAAAVLAV"
     gene            4401..5039
                     /locus_tag="WHT44_000940"
     CDS             4401..5039
                     /locus_tag="WHT44_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ2533143.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMGLSDILGRLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CLETNGLNPAPPQIDYVATSPKACYAAKYENSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDASVHWQLSVQTHKWAGIE"
     gene            complement(5374..5450)
                     /locus_tag="WHT44_000941"
     tRNA            complement(5374..5450)
                     /locus_tag="WHT44_000941"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5414..5416),aa:Pro,seq:cgg)
     gene            complement(5678..6763)
                     /gene="nagZ"
                     /locus_tag="WHT44_000942"
     CDS             complement(5678..6763)
                     /gene="nagZ"
                     /locus_tag="WHT44_000942"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ2533144.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAVGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMSTLGEIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHRNPEAVARLALAL
                     QKGLTKGGMKSCGKHFPGHGFVEGDSHLVLPEDWRSLSELETADLAPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELREDFRIPDNPTLAQRWQYMANTLGSAAAQAVMQTAD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            complement(6820..8340)
                     /locus_tag="WHT44_000943"
     CDS             complement(6820..8340)
                     /locus_tag="WHT44_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ2533145.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRTKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            complement(8542..10041)
                     /locus_tag="WHT44_000944"
     CDS             complement(8542..10041)
                     /locus_tag="WHT44_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ2533146.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFAQLVQSEGPAVVNIQAAPAPRTQNGSGNAENDSDPIADNDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLIMRRGNTLFIALNLQ"
     gene            complement(10179..10808)
                     /gene="nth"
                     /locus_tag="WHT44_000945"
     CDS             complement(10179..10808)
                     /gene="nth"
                     /locus_tag="WHT44_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ2533147.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            complement(10854..11267)
                     /locus_tag="WHT44_000946"
     CDS             complement(10854..11267)
                     /locus_tag="WHT44_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ2533148.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFTWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
BASE COUNT         2653 a         3013 c         3160 g         2485 t
ORIGIN      
        1 gttttttcga tacggaaccg gatagaggaa aaatcgaaca ttgcgcctgc cttgctatga
       61 ttcacgaaga aatctccgcc atgcctatgg gctatgaaac cttaatcggc gatatgggca
      121 gcgcactgtc aggcggacaa aaacaacgca tcgtattggc gcgggcctta tattgcgaac
      181 cgaaaatcct atttttagat gaagcgacca gccatttgga tattgccaat gaaaaagcag
      241 tcaatgcaaa cttgaatggc ttgtctatca taaaaattat ggcggcacac agaaaggaaa
      301 cggtggaatc agcagatagg aaaatgtctt taggataaaa atacagtttc aaaaatactc
      361 aagattgctg ctgttttttc gcctgagcgt caaactctgc cagcgtcatg ttcaaagtct
      421 gcaaacacgg catcattacc gcatcgacag cttggttcac atgatccctt tccacaggca
      481 acggacggta aacgaagagc ttgaagagtt cgttcaactc aatcgaatcc gcccccgttt
      541 tcaacaccca accctgtctg ccggaataga tgtagccgtg ccgcgccagc ttttccaaaa
      601 gctcgcccaa ctcgtcgtag cccatattga tatgccgtct gaactcctga acaggcaagg
      661 ctttgccttc tttttgcgcc gcatccagaa gcagcaggat tttcaacacg tcgtcaaacc
      721 gtccgcgcga gtcgaagccc ctgcggaacg cttctccctg ccagtaggag agtgaagaag
      781 tcagcaccgc gccgcccaag accagcgtcc acaacaggtt cagccacaac aggaaaaacg
      841 gcacggcggc aaacgcgccg taaatcgagc ggtagccgtc gaaattgccc atataccaag
      901 taaagaggga acgcgcggtt tccagacaga acgctgttgc caaagccccg acaaacgcct
      961 gccgcgcggg aacgaagcgg tttggcacga agcggtacag cccccacagc aaaagcgtca
     1021 tgaaggtcag cgtcgccgcc gttcgcaacg cgcccgacca ctgcggcgca cctgaggcaa
     1081 gcgcggcatc ctgtaccgag ccgaccataa aggaaatgcc cacgcccaaa gacagcggcc
     1141 cgaacgtcag taaagcccaa tagacgagaa actgcatcat ccacggacgc tgggaattga
     1201 cccgccagat gcggttgaac gtattgtcta tcgtccgaat cagcatcagc gaggtaacga
     1261 ccagcatcac gctgccgatt gccgtcagcc ggttcgcctg ctcgcggaac gcattgatat
     1321 agtcgaacac catgtccgcg ccctgcggca caatggtttg gttgacgaag gagacgaacg
     1381 aatccgacca gcggtcgaac acggggaaaa tcgaagcgac cgccaccatc acggtcagca
     1441 cggggacgag tgccagcagc gtcgtaaacg tcatgcttgc cgccgcctgc ggtacgcgtt
     1501 cttcatcaaa gcggcggacg acgaaccatg caaacgcaca gattttattg tctgccaaac
     1561 cttgcaaacg ttgtaaaaag gtcataattt cttgcccggt cagtaagttg ggcattgatg
     1621 cccgatgtta tagccaattt tgccgtcagg aacaaatgcc tgaactgcgg ctgtttcaga
     1681 cggcatcgga acaactgtta tgccgtctga agaccgaacc attttaacgg aatccgccca
     1741 tgaacccaaa tccccctcaa aatcctcgtc ctctactatt tccaaaacgg cagcacccgc
     1801 aatcccgccc gccgaatcgc tcgcggcatc gacagcgttg aaggttgcga agccgtattg
     1861 cgcaccgtcc ccaaagtctc cgccgtctgc gaagccgtca aaaaagatat tcccgacagc
     1921 ggctcccgtc ctgaccgccg aagaaaacaa tatcgccttc gcacaaagca aacgcttggc
     1981 ggaactcgcc gtcaagtcgg cataagccgc gtgttcagac ggcatggcgt tcagatgccg
     2041 tctgaacacg tttgcctgta taatccgcat ctttactgtc caacttcgcg gttcgcaaac
     2101 ctcccgcgtt accaaaacta ggattcgata tgtcaaacca acaagccttg gtcatctttt
     2161 cgggcggtca ggattcgacc acctgcctga ttcaggcaat ccaaacctac gggcgcgaaa
     2221 acgtccaagc cattactttc caatacgggc aacgccatgc cgtcgagctg gaacgtgccc
     2281 gctggattgc gcaggatttg ggcgtcaaac aaaccgtact cgacttgagc ctgatgcggc
     2341 agattacgca caatgccctg atggacgaca ccgccgccat cgaaactgcc gaaaacggcg
     2401 ttccgaatac ctttgtagac ggccgcaacg cgcttttcct gctctatgcc gcgatttacg
     2461 ccaaagggca ggggatacgg cacatcatcg cgggcgtgtg cgaaaccgac ttctccggct
     2521 atcccgactg ccgcgacgtg tttgtcaaat cgatgaacgt tacccttaat ttggcgatgg
     2581 actatgattt tcaaatccac acgccgctga tgtatctgac caaggcgcaa acgtgggcgt
     2641 tggcggacga aatgggcgtg ctggactata tccgcgagca aacccacacc tgctataacg
     2701 gcatcgtcgg cggctgccgc gaatgcccga gctgtatctt gcgcgaacgc gggctggcgg
     2761 aatatctgga aagtaaaaag gccgtctgaa cacgcgcaaa ccataaggaa tacgatatgc
     2821 ccaagctcca tatgttttac ctcggcggca atgccggcag gtcgaatatc gaagtgcacg
     2881 acatccaatt tgccgtgtgc gacaactacc gcgaggccgt ccccgcgctc aaagccgcgt
     2941 ggttcggcga tgcggacaaa atccacatcg acggctggca gattgtcgaa tgggcggacg
     3001 gttacgacat cgccgtatcc gaaacgccca aaacgaaaat gccgtctgaa cacgccccgc
     3061 gcctgtattt cgccaatgtc ggcggttatc gcgcgggtca gcttgccgag gcacacgctt
     3121 tcgggctgtt cgccgccgcc acgcctgccg aagccaaaca aaaagccctg caaaccctgt
     3181 tgaccgacag ctatgttcag cagcataaag acaacttaaa agacgtggac aacctgcttg
     3241 cgctcgaccg catcggcaat ttccatatcc gcctgacccc gaatccgcac ggcaaacccg
     3301 ccgaaatcgg ctttcaaggc tatttgccca tttgagaacc catgaaaatc accaaaatct
     3361 tcaccttcga ctcctcgcat atgctcgacg ggcatgacgg caaatgccaa aacctgcacg
     3421 gacataccta caaactcgaa atcaccgttt cagacggcat tatcaaaggc ggcgcgaaag
     3481 acggtatggt gatggacttt accgacttga aagccattgt caaacaacac attaccgacc
     3541 ccttcgacca cgccttcatc taccacggcg gcaacagccg cgaatgccaa atcgccgcgc
     3601 ttttggaggg ctggaacatg aaaaccctgc gcctgccctg ccgcaccact gccgaaaata
     3661 tggcggtcga aatgtacggc cgtctgaaaa acgcggggct gaacgtgtgc cgcgtgaaat
     3721 tgtgggaaac gccgacatcg tgtgcggagt atgaagggga gtagggaata tcttgaacgt
     3781 atcgatatag taaattccaa taagacatgc ccaaccgcgt cattcccgcg caggcgggaa
     3841 tccagacctt gatttatcag gaatatttaa aaattgcagc aattccaact ctctggattc
     3901 ccgcctgcgc ggaaaggacg gtttagagcg tccttatttg aatttaccgt aaaacggttt
     3961 tttctcctgt acggattccc cgttttttca gacgaccttc catatcaaat acacccatta
     4021 aaaggaatac ccatgaaact cctcttcatc ctcctagtcc tcttcgtcgc cgtcgaacat
     4081 ttctacatcg cctggcttga aatgacacag attcccagcg aaaaagcggc ggaaatattc
     4141 aagctgcctt atgaatttat ggaacaaaag caagtgcaga ccttgttcag taatcaaggg
     4201 ctgtataacg gctttctcgg catcgggctg gtgtggtcgc ggtttgccgc gccggacaac
     4261 gccgtttacg gcgcgacgac tctgtttctc ggtttcgtat tgattgccgc cgcgtggggc
     4321 gcgttttcgt ccggcaacaa aggcatactc gtcaaacaag gactgcccgc gatgctggcg
     4381 gcggcagcgg tgttggcggt atgaaaaaaa tcaatgttgc ccccgaaaat ccgcaatacc
     4441 gtatcgtcga aattttcgag agcctgcaag gcgaaggctg gaacacgggc atgcccgccg
     4501 ttttcgtccg cttgggcaaa tgcaatctgg cgtgcggctg gtgtgatacc gattatttga
     4561 cattcggtat gatgggcttg tccgatatct taggccgtct gaaaacctac gccgcccgca
     4621 acatcatcat caccggcggc gagccgacca tacagccgca tctcgatatg ctgctggaca
     4681 cgctcaaggc ggaaggctat ttcctctgtc tcgaaaccaa cggactcaat cccgcgccgc
     4741 cgcaaatcga ctacgtcgcc accagcccca aagcctgcta cgccgccaaa tatgaaaata
     4801 gctgtatcga aacagccgac gaagtgcgga ttgttgccga tggtgatgtc cttgcgttct
     4861 gcgaaaacat ggaacgcaaa atccgcgcac atcattacta cctttcgccc tgtgagcaag
     4921 acggtgcgat gaacatctac gacaccatcc gccaaatcgg tattttaaac agtcgccccg
     4981 acgcatccgt gcattggcag ttgagcgtgc agacgcacaa atgggcggga atagagtagt
     5041 ttaagcagtg taactcaaag ggacggcgta cggttttacc gatgtttgac atacggggaa
     5101 agtgtgccgc ttctgcgtgg aaatgccggc atttccaccg cccaatcagg acggagcctt
     5161 actgaataag atgctgccgt tgggtacaag ctcggcttcc taaattccga tggtcttttg
     5221 aaccttgccg atactctgtg ccagtgcgcg caaatggcag ggttagggaa aacgaaatgc
     5281 cgtctgaaac agcattctgt ttcagacggc atttttctgt tgccgccaaa aggaaaaacc
     5341 gcctcggcaa tggatgccga ggcggtttga atatggtcgg aatgagagga ttcgaacctc
     5401 cgcccccttc gtcccgaacg aagtgcgcta ccgggctgcg ctacattccg aattaagtaa
     5461 ggcgtgatta tagcgcaaaa agtgcggcgt gcctataccg ttttgccttt ttgccgcgtg
     5521 tcgggcggat ttaaaacgtt gtgtttgaat acagtgttga taatcatcat tatctttaag
     5581 taattcaata agataacttt ctacctgacc gaaaaaatca ttgcctttcc ctgacaaacg
     5641 gttgatgaaa tcggcagatt gttgaaacgc agccggttta aaaggcttcg ccgactttca
     5701 cgccgcccgc cgtgtcctgc ggcgaggcaa ggccggcaac aaaggcttgc gccgcttgga
     5761 aatccgccgt ctgcatcacg gcttgcgcgg cggcactgcc gagcgtgttg gccatatatt
     5821 gccaacgttg cgccaaagtg ggattgtcag gaatgcggaa atcttcgcgc agttcatcca
     5881 caaggtcggg acggttgcag acgaggacga tgtcgcaacc tgcctcaaag gaaatgcggg
     5941 cgcgttcttt gatgccgcct gccccgcacg cgccctccat agtcaaatcg tccgagaaaa
     6001 tcacgccttt gaacccgatg tcgcggcgca aaatttgttt gagccagatt tcggaaaacc
     6061 ctgcgggctt tgtgtccact tgtggataaa cgacgtgggc gggcataacc gccgccatac
     6121 cttcgcggct cataatgcgg aagggggcga ggtcggcggt ttcgagttcg gacaggctgc
     6181 gccagtcttc cggcaagacc agatggctgt ctccttcgac aaatccgtgt ccgggaaaat
     6241 gtttgccgca ggatttcata ccgccttttg tcaaaccttt ttgaagggcg agggcgaggc
     6301 gggcgaccgc ttcgggattg cggtggaaac tgcggttgcc gatgacgggg cagtttcccc
     6361 agtccaaatc taagacgggc gtgaaggaca aatcgatgcc gcaggcggaa agctcggttg
     6421 ccaaaacccg gccgacttgt ccggcggcgg tttcggcggc ggacgcgccg tctttgtccc
     6481 aaatctcgcc gagcgtactc attgcgggca ggcgggtgaa gccttcgatg aaacgttgca
     6541 ccctgccgcc ttcgtgatcg acggcgataa tgagttcggg tgtgcgcagg gctttgattt
     6601 cggcggtgag tgttttgagt tgttcgatgt tttggaagtt gcggcggaag aggatgatgc
     6661 cgcctacggc gggatcgagc aggcgttgct tttcctcttc ggtcaggcgg aaggcggcaa
     6721 tgtctgccat gacggggccg cgcggaatat gggggacggt cattgcggtt tgctccaaaa
     6781 agcttcagac ggcatatgcc gtctgaacag ggaaaggggt caggcgttgg cgcgtttttt
     6841 atctttcaac agaaaaatca gcaccgccaa tacaatgcct gtcgtgccaa agcccaacag
     6901 cgcggatttt gtcagaccca atgcgaggta gcccgatgcg gcggcggcgg caacggttaa
     6961 ggcgtaaggc agttgcgagg taacgtggtc gatgtggttg cagcgcgcgc cggtggacga
     7021 caggatggtc gtgtcggaaa tgggcgagca gtggtcgccg cataccgccc ccgccattac
     7081 tgcggacata cacgggataa tcagcgcggg ttcgactttg accgccatgg cggcggcaat
     7141 cggcagcata atgccgaacg tcccccagct tgtgcctgtg gcaaacgcca tcacgctggc
     7201 gagcaggaag aggatgacgg gcaggaagcc gggatggatg ttgcccgcaa ccagtgtgga
     7261 gaggtaatcg ccggtgtgca tttcgccgac aaccgtactg atgagccaag cgaggattaa
     7321 aatggcgatt gcgccgaaca tagatttcgc accctgccaa acggctttgg gatagtcggc
     7381 ggttttaatc gtgccgagcg tgcagagaac gacggcaagg acgccgcaag tgccgccgaa
     7441 taccagcgaa gtgtttacgt ccgtgttttc aaatgccccc aaaatgctga aggtttcgct
     7501 tgcctgcgcg ccggtgtaga tcatggcgga aaccgttgag gcgattaagg ccaaaacggg
     7561 aataatcagt gcgtaaacac gacctttggt agcgtctgaa acggcagttt catcgtgggc
     7621 ttcgttcaac gcggcttgtt cgaaacgtgc catcgagccg atgtcgaagg aaaaccatgc
     7681 gacgacgaac accataatca gggcaaacag tgcgtaatag ttcatcaggc tcatggcgac
     7741 aaacgtcccc atcggcgtgt attcggtgat tttgtaggta acgagcagtc cggcaagcgt
     7801 ggcgataatc gacgcgcccc agcttgaaac gggcatcagc acgcacatag gagcggcagt
     7861 ggagtcgagg atgtaggcga gtttggtgcg ggaaacttta aacttgtcgg taacggggcg
     7921 ggcaatcgca ccgacggcga gactgtggaa atagtcgtcg ataaaggtta cgaacacgag
     7981 gcaggcggtc agcattttcg cgccgcgccg gtttttaatg tgccgttttg cccagtcggc
     8041 aaacgcctga ttgctgccgg agtaggtcag cagggaagta aaaataccca aaagtatcag
     8101 gaaaaccaag atttttggtt tgcccagcga ccaatcgccg tctgaccaag ccaagccgac
     8161 gaccatgtct ttcaggtgtg tcagaccgtc gacggggttg ccgccgacca aaaaggcaac
     8221 gccgaccaga ataccgatgc ctaaagacag cagtacgcgg cgggtaatga cggcaagtgc
     8281 cagtgccaaa aagggtggca caaccgagaa aaatgaatgt gaatagtcga tcagctgcat
     8341 ggttatgggg gtgttaagcg tccggatggg agcgtatctg tccgcctccg gtttgggttt
     8401 tgttggcaaa atgggcggaa atattttttg tcgtaaaaaa tatttgttta aaatcaacca
     8461 actgattttt gtaaaatgcc cgttaatcgg tattgacggg cattttatca tttaaaaaat
     8521 attttggtta aattatgtgt gttattgcag gtttaatgcg ataaacagcg tgttgccacg
     8581 gcgcatgatc agcaggggga cgtttttgcc tgccttgtcc atagctttgc ggaaaccggc
     8641 ttcgtcattg acggggactt gcccgacggc aagaatttcg tcgccgcgcc tcaagcctgc
     8701 gcgttctgcc gcgtcggaaa cccgtacgac gacgaggtgt ccgccgctgc tgtcggtatg
     8761 tgtctgaagg gtaatgcctg cggattcgac cgagaacgta ccggattgct gttcggtgta
     8821 gggggcttca tctgttttgg atgatgcgcc gatatgctcg gcggcgttgc ccagcttgac
     8881 tttgattgtg atttcttcgc ctttgcgcca tacgccgagg ctgacttctt ttcccggcgt
     8941 aatggcgccg accataacgg gaaggtcgcc ggaagaacgt atttctccgc cgtcgaggct
     9001 gaggacgatg tcgcccgcct gcaggccggc acgttctgcg gggctgccgg gcaggatttt
     9061 ggcaatcagt gcgccgccgg ctttgtccaa accgaacgat tgtgccaaac cgtaggatac
     9121 ttcttgaata atcacgccca gttgtccgcg ttggactttg ccggtgtttt tcagctgttc
     9181 ggcgacattc atggcaacgt caatcgggat ggcgaaggaa atgcccatga atccgccgct
     9241 gcggctgtat atttgcgagt tgatgccgac gacctgtcct tttaagttga acagcgggcc
     9301 gccggagttg cccggattga tggcaacgtc ggtttggatg aagggtgtgt agctttcgtt
     9361 gggcaggctt ctgcctttgg cggacacgat gccggcggtc acgctgttgt cgaagccgaa
     9421 gggcgcgccg atggcggcga cccattcgcc cggtttcaaa tctttgggat tgccgatttt
     9481 gacgacgggc agctcttccg ttgcgtcgat tttcagaagg gcgacatcgg attggacatc
     9541 cgaaccgatg agtttggcgg tatattcgcg cttgtcgttg agcaggactt tgatactgcc
     9601 catgccggta acgacgtggg tattggtcag gatgtagccg tctttgctga tgatgaagcc
     9661 cgaaccgaag ttcaatccgc cgtcatctgc ttcttcttgg gggatttcgg gcatattcgg
     9721 gacgaggcgt ttgaaaaatt cgtagaacgg gtcgttgtcg gcaatcgggt cggaatcgtt
     9781 ttcggcattg ccgctgccgt tttgggtgcg cggggcgggg gctgcctgaa tattgacgac
     9841 tgccggacct tcactttgaa ccagttgggc aaagtcgggc agcagcatac tgacgctgcc
     9901 gtcgtctttg gtgtgttcga tgcgttctac gaaggatgct tcttttttgt ccgcaccgaa
     9961 aaagctgcct gccttgtcgc agcctgccag cgaggcggca cacagtgctg ccaaagcgag
    10021 gtattggtat tttttgaaca cgttttgtcc tttgtcggat gccggtaccg gctttaatgc
    10081 cgtctgaagc gcattttgtc ggcttcagac ggcataggtt gaaattctac aacgtccgtc
    10141 cgaattttca agcgtttcat tttgaagggc ggcggcggtc aggctttggc gggatattcg
    10201 cacaaatcgt tgatgatgca ggtttggcat tgcggtttga gtgccttgca ggtgtagcgt
    10261 ccgtgcaaaa tcagccagtg gtgcgcgtcc atcagaaatt ctttaggaat gaagcgcatc
    10321 agtttgtctt cgacttcgcg cacatctttc ccgggggcga ttttggttcg gttggatacg
    10381 cggaaaatat gcgtatcgac cgccatgacg ggatggccga acgccgtgtt caatacgacg
    10441 tttgccgttt tgcgccccac acccggcaat gattccaaag cctcgcggtc ttccggcact
    10501 tcgccgttgt atttttccag caggatgcgg caggtttgca taatgtgttt ggatttggtt
    10561 ttatacagcc cgatggtttt cgtgtattcc atcacgccgt ccaaacccaa atccagcatc
    10621 gcctgcggcg tatcggcaac gggaaacagc ttcgccgtcg ccttgtttac gccgacatcg
    10681 gtcgcctgcg ctgaaagcag aacggcaatt aaaagctcga aaggggagtt gaaattcagc
    10741 tcggtggtcg gatgggggtt ggcggcgcgg aagcgttcga agatttcttg gcggatgtgt
    10801 ctgttcattt ttttatacgg tgggtttgtg tgttcggcat tataacgtat ggttcaggcg
    10861 gcgtaatatt gcattcccca cagaatgaag gcgtaacgcg ccgttttgcc gataaccagc
    10921 atcagcccgc ttgtccacgg attcaaccgc agccagccgg cggcaagcgg cagtgcgtcg
    10981 ccgacgacgg gcagccaggt aaacgcaagc agccaaatac cgaaacgccg catcagattc
    11041 agtgtttttt cagacggcat ttttcgggag ggcagcaaac gccccatcca ataggaaacc
    11101 atactgccca atccgttggc aaggccggcg cacagcaacg cgccgtatgc gtgttcggga
    11161 aagcggtgga cgaacagggc aaaggcggct tcggatgtgc cgggcaggag ggtggcggaa
    11221 gtgaatgcgg aaaaggcgag ggcggcgtag gtgtaggagg gtatcattgc aaacagtctc
    11281 aaacaggtaa caatcggcga cggattgtac g
//
LOCUS       DAWGRZ010000050        11302 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000050 DAWGRZ010000000
VERSION     DAWGRZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.050"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(80..1126)
                     /gene="recA"
                     /locus_tag="WHT44_000947"
     CDS             complement(80..1126)
                     /gene="recA"
                     /locus_tag="WHT44_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ2533149.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEIADEIDAKIRALNGIEMHIT
                     EGTQDETDGERPEE"
     gene            1380..2144
                     /locus_tag="WHT44_000948"
     CDS             1380..2144
                     /locus_tag="WHT44_000948"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ2533150.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEILHHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRCAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKELAAKPIVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLENGAD"
     gene            2164..4179
                     /gene="rep"
                     /locus_tag="WHT44_000949"
     CDS             2164..4179
                     /gene="rep"
                     /locus_tag="WHT44_000949"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ2533151.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYQLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQTFMDMFVSYLAKAETSEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD"
     gene            complement(4247..5305)
                     /gene="dinB"
                     /locus_tag="WHT44_000950"
     CDS             complement(4247..5305)
                     /gene="dinB"
                     /locus_tag="WHT44_000950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ2533152.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYAGDVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVSPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
     gene            complement(5449..8538)
                     /locus_tag="WHT44_000951"
                     /pseudo
     CDS             complement(5449..8538)
                     /locus_tag="WHT44_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212999.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            8812..9591
                     /locus_tag="WHT44_000952"
     CDS             8812..9591
                     /locus_tag="WHT44_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ2533153.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALTEHPLVVEYGHSFRFLPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKFTPES
                     TRFMICGNPDMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(9801..11213)
                     /locus_tag="WHT44_000953"
     CDS             complement(9801..11213)
                     /locus_tag="WHT44_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ2533154.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVVGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRVGIGFSSPVLCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFP
                     ALGSLHAYKIKAELVGRYHSGCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVT
                     DNPPTGLFANKKAARRALSSWAETYGLCPAAAGILSDGYTEDEPCPVYVSDRCDKACV
                     RSDEQVLAFAHKLPVLDWGKMHEVEITETDPLTGEKVVLHGMGGALEMDDGLWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
BASE COUNT         2754 a         3164 c         2728 g         2656 t
ORIGIN      
        1 aactcaaatc aagccgttcg gaggcagctc aaaaaacggt acttcgcagc agaagtaccg
       61 tttatcggga tttcaggttt tattcttcgg ggcgttcgcc gtcggtttcg tcctgcgtcc
      121 cttcggtgat gtgcatttct atgccgttga gggcgcggat ttttgcgtcg atttcgtcgg
      181 caatttcggg attttccttc agccagacgc ggacgttgtc tttgccctga ccgattttcg
      241 cgccgttgta gctgtaccac gcgccggatt tgttgatgat gtcgtttttc acgccgatgt
      301 cgatcaattc gccttcccaa ctgatgcctt ctccgtagag gatgtcaaac tctgcctgac
      361 ggaacggcgg cgcgactttg tttttgatga ctttgacgcg ggtttcgttg cccaatactt
      421 cttcaccttt tttgatggat ccggtgcggc ggatgtcgag gcggacggaa gaatagaatt
      481 tcagcgcgtt gccgccggtc gtggtctcgg ggctgccgaa cattacgccg atcttcatcc
      541 ggatttggtt gatgaacaca accagcgtgt tggttttttt gatgtgtccg gtcagtttgc
      601 gcaaagcctg gctcatcagg cgcgcctgca gtccgacatg gctgtccccc atatcgcctt
      661 cgatttcggc tttggggacg agtgcggcta cggaatcgac gactaccata tctatgccgc
      721 ccgaacggac gagtgtgtcg cagatttcca aagcctgttc gccggtatcg ggctgggaca
      781 ggtaaagctc ttcgactttt acgccgagtt tgcgggcgta aacgggatcg aaggcgtgtt
      841 cggcatcgac aaaggcgcac acgccgccgt ttttctggca ttgggcgacg gcttcgaggc
      901 agagggtggt tttgccggag gattctgggc cgaagatttc gacgatgcgc ccgcgcggca
      961 gaccgccgac tccgagggcg aggtctaatc cgagcgatcc ggtggaaatg acttcgaggt
     1021 tttcttcctg ctggctgccg tccattttca tgatggcgcc tttgccgaaa cttttttcga
     1081 tttgcgccag tgcggcggca agtgctttgc ttttgtcgtc tgacattggg gttactccgg
     1141 aacaaatgcg gtatgtggga tgcggcgcaa cacgggctgc ggcgcgggat gtgtatcgtt
     1201 ttcccgatgt gcgggctatc ggtaatgctg cttcacgagg ttgccattat cgcatatttc
     1261 cttgcttgcc gatatgcgcc aggacgcggc ggcttgtgcc ggaatggaat ctggatgccg
     1321 tctaaaaggc ggccggcttt gttataatgg cggctgtttt ttctgtgtgt gcctgtttta
     1381 tgtgttcctg ccttgttgtc aaaaataccg ttatcggaag cggacgcacc aaaatcgccg
     1441 tgccgcttgt cgcccgcgat gccgccgaac tttccgccgt acttgagcaa atcaaaaata
     1501 tgcccttcga tattgcggag ttccgcgccg actttttgga atgcgcgggc agtatcggcg
     1561 aaatattgca ccacacgcag accgtccgcg acgcgctgcc cgacaagccg ctgctgttta
     1621 cgttcagacg gcatggcgaa ggcggctcgt tcccgtgttc ggacgattat tattttgaac
     1681 tgctcgacgc gctgatcgaa agccgcctgc ccgacatcat cgacatcgag ctgttttccg
     1741 gcgaaaccgc cgtccggtgc gccgtggcaa atgctcaaaa aaacggcatc gccgccctgc
     1801 tctgcaatca tgagtttcac cgcacgccgc cgcaagaaga aatcgtatgc cgtctgaaac
     1861 agatggagga ctgcggcgcg gacatctgca aaattgcggt gatgccgcaa agcgcggaag
     1921 atgtgctgac tttgctttcc gccacgctca aagcgaaaga gcttgccgcc aaaccgattg
     1981 ttacgatgtc gatggggcag acgggggcgg tcagccggct tgccggacag gtgttcggct
     2041 caagcatcac gttcggttcg ggaacgcaaa actccgcgcc ggggcaaatc ggcgtatccg
     2101 ccctccgtgc gacactcgac tgcctcgaaa acggcgcaga ctgatttcag acagcatcaa
     2161 aacatgatga aactcaatcc ccaacagctc gaagccgtcc gctacctcgg cggcccactg
     2221 ctcgtccttg ccggtgcagg cagcggcaaa accggcgtga ttactcaaaa aattaagcat
     2281 ttgattgtca atgtcggcta cctgccgcat accgttgccg caattacctt taccaacaaa
     2341 gccgctgcgg aaatgcagga gcgcgttgcc aaaatgctgc ccaaaccgca aacgcgcggg
     2401 ctgacgattt gcacgttcca ctctttgggc atgaagattc tgcgcgaaga ggcgaaccat
     2461 attggttaca aaaaaaactt ctccattctc gattctaccg acagcgcgaa aatcatcggc
     2521 gaactcttag gcggtacggg caaagaagcc gtattcaagg cgcagcacca gatttccttg
     2581 tggaaaaacg atttaaaaac gcctgaagat gtcgttcaga cggcatcgaa catttgggaa
     2641 caacaaaccg cacgcgtgta tgcgagctat caggaaacct tacaaagcta tcaggcagtg
     2701 gacttcgacg acttaatccg cctgcctgcc gtgctgttgc agcaaaacag cgaagtgcgc
     2761 aacaaatggc agcggcggct gcgttatctg ttggttgacg aatgccaaga tacgaatacc
     2821 tgccaattta cgttgatgaa gctgctgacc ggcgcggaag gtatgtttac cgccgtcggc
     2881 gacgacgacc agtccatcta cgcatggcgc ggtgcgaaca tggaaaacct gcgtaaaatg
     2941 caggaaaact atccgcagat gaaggtcatc aaactggagc aaaactaccg ctccaccgcg
     3001 cggattctca aaatcgccaa caaagtcatc gaaaacaacc ccaagctgtt taccaaaaaa
     3061 ctttggtcgc aattgggcga aggcgagccg gtcaaagtcg ttgcctgcca aaacgagcaa
     3121 cacgaagccg actgggtcgt cagccaaatc gtcaaacaaa aactcatcgg cggcgacaaa
     3181 acccaatatg ccgatttcgc cgtgttatac cggggaaagc atcaggcgag gattttcgag
     3241 gaagcattgc gcggcgcgcg catcccctac cagctctccg gcggacaaag ctttttcgac
     3301 aaagccgaaa tcaaagacgt gttgtcttat gtgcggctgc ttgccaaccc caacgacgat
     3361 cccgcctttc tgcgtgccgt taccacgccc aaacgcggca tcggcgatgt cacgctgggc
     3421 aagctcaaca cttacgcgca cgaacacgaa tgcagcctgt atgaagccgc gcaaaacgaa
     3481 gaagcccttg ccacgctgaa caataccaac cgccaacacc tgcaaacctt tatggatatg
     3541 ttcgtcagct acctcgccaa agccgaaacc agcgaagcgg gcgagttcat caacagcctg
     3601 ctcgaagaaa tcgactatga aaaccatttg atgcaaaacg aagaaggcaa agccggcgaa
     3661 atcaaatggc gcaacgtcgg cgatttggta tcatggtttg cgcgaaaagg cggggaagac
     3721 ggcaaaaaca tcatcgaact cgcccaaacc gtcgccttga tgacgctttt ggaaggaaaa
     3781 gacgaagaag aaaccgatgc cgtctcgcta tccacgctac acgccgccaa aggtttggag
     3841 tatccgtatg ttttccttgt cggttgcgaa gaaggcgttt tgccgcacaa cgacagtatc
     3901 gaagagggca acgtcgaaga agaacgccgc ctgatgtacg tcggcatcac ccgcgccaaa
     3961 cgccaactca cactgaccca ctgcgtcaaa cgcaaaaaac aaggcacatg gcagttcccc
     4021 gaacccagcc gattcataga cgaaatgccg caggaagatt tgaaaatcct ggggcgcaaa
     4081 ggcggcgaac cgattgtcag caaagaagaa ggcagacgca accttgccga tataatcgga
     4141 aggctcgaca acctaaaaaa aagcggcgcg gcggattaaa ccggagccgc aatgccgtct
     4201 gaaggcttca gacggcatat tttttggacg gcgcgcgtaa agcggtttac gcccacaaat
     4261 cctgctgctg gtttttcggc acaagatgcc ccacgccgat accgataagg cggaacgcgt
     4321 cttccgtctg cggcgagacg cgcgccatca acatttgcgc agcctgcagc agagctgcgc
     4381 agtcgggcaa tacggaggaa taagtcagtg tgcgcgtgat gatgcggaaa tcgtaggtct
     4441 tcagcttgag cgttacgctt tgggcttcga cgtttttgcg cgtgatttgc cgccacaagt
     4501 cttcggcaag atgggggagg tgtccggcag cctgctcgag cggcaggtct tcgggcaggg
     4561 taatttctgt ggagatttgg aggcgttcgc gttcggcttt gacggggcgt tcgtccgtac
     4621 cgcgcaccaa atcatagagg cggtatccgt agcgtccgaa atggtttaag agttcgccgc
     4681 gctcgaaacg gcgcaagtcg cccgccgtcc gcatacccag cgactgcatt tttttcagcg
     4741 ttaccttgcc cacgccgggg attttgccca aaggcagggt ttccaaaaat gccatgactt
     4801 tgtgcggcgg caacacaaac tgcccgttcg gcttgcgcca gtccgacgcg attttcgcca
     4861 gaaatttgtt cggcgcgatg cctgcggatg cagtcaaacc tgtttccgca aaaatggcgg
     4921 cacggatttc tttggcaacg tcgccggcgt aagggatgtt tttgaaatta cgggtaacgt
     4981 caagataggc ttcgtccagc gacaagggtt cgattaaatc ggtataacgc ctgaatacgg
     5041 cgtgaatctg cgcggaaacc tgacggtaca aatcgaaatg cggcggcaca tacaccgctt
     5101 gcggacacag ccttttcgcc gttgccaccg acatcgcgga atgcagcccg aactgccgtg
     5161 cctcatacga tgcggcgcaa atcaccgaac gcgcgccctc ccacgcgacg accaccggcc
     5221 gccctttcaa atgcggctgt tcgcgcagct ctaccgatgc gtagaatgcg tccatgtcga
     5281 tgtggataat tttgcgtgaa gacatcggct cttctgagga taaaagggat attctactgc
     5341 cggcatcggg caaattccaa atatacgccc cgatagacct gcctccataa aaatgccgtc
     5401 tgaaacatac cctgtttcag acggcatccg caaaactacg gttttcaatt aaaactgcca
     5461 atccagtttc atgctgacag tgcgcggctc tccgtagaag ttgtttgcgc cgcgcgtacg
     5521 gttgtagttg ttctcaaaat aagtgcgtcc gtttaagttc gtaccgatga ggctcaattt
     5581 ggcgtgtttg cccaattcgt aacggacgaa accgtctatc agcccgtagc cgccctgcct
     5641 gatgttatac agactgcttg tgccgctttg tgcggacacg ccgccgccga cggtcagccc
     5701 cgtattcggt atatggaagc tcgttccgaa acggaatatg tgcacgggtg tgaaattgct
     5761 gaagttgtac gggtctgcac tggaattttt ggcaaggcgt tcggcgttga cttcggcggc
     5821 gtttttgtag cggctcttgt tgtaggtgta acccgcaaag actttccaat cttcgttcaa
     5881 ctcacccgac aactcgaatt ccgcacccct gctgaccact ttgcctatcg gtttggcaac
     5941 ggtttggaac gacccctgct tgccgcctgc tccgggaaca tagccgaaat cgacgaccgt
     6001 gcggtttttc tgttcgaggt aaaacaatgc gaacgaagca ttcagccgtc cttgcaagaa
     6061 cgcgcctttc cagcctacct catagtttgt gccgaccaaa ggcggtaaaa cggttttggc
     6121 actgacatcg acattatcct gctgtttgaa gattttggta taacttccgt aaatactctg
     6181 ttgcggtgtc aagtcatagg taatgcctgc atagggcgtc aatttatgac cttgcatctt
     6241 ggccgtgtaa tggtcctgat ccgccctaat gctcgatgcc gtctgaaaat cgcttgccgg
     6301 ctgcccatag cggacaggca tatctttggt ttgcgaagtc tcatagcgcg tgtagtgcag
     6361 cccgcccaaa aggtgcagtc ggccggttac gttgaaacgc gtgctggcag tcagcgaatg
     6421 ggttttgttg gtgttgaggt atttggcgta gttatacagc gcaggaacat ggtcgtctgc
     6481 cactttgacg gttttccaaa ccggcaccgt accggaaaaa ccggtaaagg caggcgtgcc
     6541 gtcgggattg gtctcctgaa tcttgttgcc tttttcgtcc agctcatata catcgacata
     6601 taccggtgtc cggctgccgc tgtattcgtc atagtaatac acctgcttgc cttcggcatc
     6661 gagcttgggc tcggttttta ttttcttggc gttcctgcat tcttcggcat aaacggtacg
     6721 gttgcctttt tcatcgtacg cctgccaatc gggttcttta tgccccctga ccaaaggaga
     6781 cgacaaatcg ccgtccggct cctcctgaca acttcccgca tacacgccgt gcgttgcccc
     6841 cgtattcgga cgtactctgt agcggcgttc gtagatttct agatattccg aacgtatctt
     6901 ttcatcaccg taggcatagc cgacaaagaa atcatgctcc cgcccgaaca gcccatatgt
     6961 gccggtcagg tcaagtttaa ttccccattg gcggtcgtct ttggtatgcc gcaacggcat
     7021 ataactgtat cgtcggttgg cggtagcctt ccgattaaag gaagagtcat acaggctgtt
     7081 tggaaaacgt tgtgccgcat tattaaagat gccctccttc gcaagggctt tatcgacaaa
     7141 ttccgccttg tcggcatcaa cgcccggatc cccccaagaa ccttgacaga taaagtccag
     7201 cgcgaaaggg tcactcatac acttgtcaaa accggctttg cgttctgcgg cacggcggct
     7261 gcgatactgt tcgaaagcag tattatcgaa acggttttta acaaaatcgt ctttgcgctc
     7321 ccggtattcc ttggtggttt catcacgata tgctttcagt ttctccaatg ccttatcttt
     7381 cggctcgaac gggatgactt cgtttttttc agtcaaaaag cctaccgcat cctcacccga
     7441 caaacccgcc gcatattcgt ttttcagaaa aaactgcccc accttcgcat cggattcatt
     7501 cttggtataa gacacttcgg cattgagctg ccaaccgttg tcaaacacat gtttgaatcc
     7561 tgagaaaagg ttgtatttgt cggcacttaa ccgcgaccaa tcctccccca aataagtgtt
     7621 gcgcggcagt tgcaaaggcc ggttgcaggc aggcgttgaa ctgaacgggg cagttttctg
     7681 attttcacag ggcaaaataa tgcccgaaaa atcaggaacc tccctactct tctgatacat
     7741 gccgcccaaa gtaagcacac tgctgtcgcc cgcatcggct tcggcaatgc cgtaaaccat
     7801 atgtttcctg ccccaaactc ggtctttaaa cgatttttta tactcttccg cacccaccaa
     7861 ccttccgcgt aaggtattcg ccttattcag gctgcctgaa acatccaaca ctgcacgccg
     7921 gctgccgcga tggtcggcgg tcagctctcc ggtatgtttg aaagaagcgg taggtcactt
     7981 acggatcaaa ttgacggttc ctcccggctc tgaattggat tgggtcaacc ccgttgcacc
     8041 ccgtacaact tcaatatggt cataaaccgc caaatcggta ctcggagaca cgtcgatttt
     8101 cgccgtatat cccgaacggc ctgcaacatt gacggtcata ccgtcttcac caatctgatc
     8161 aatatagaaa ccgcgtgaca aaaaccgcgt ctgcaagcct gaatcgcgca caacgttgac
     8221 acccgtcgtg tttttcattg cctcttcaag cgtatgcacc gccttatcgt caaggcggct
     8281 gcgcgtgatg acgctgaccg actgcggcgt atccttgccc gcaatcctca tacctgtggc
     8341 ggtggacatc cgatctatcg tataagaacg ggtcttttcg gtcttgccca acaaagcatg
     8401 agagccgcgt acattgaccg tatccagact gacggtattg ccgtctgaaa caggcacaac
     8461 accgtctgca aaagaaccac cgtaagccga taacagcata acggtcagaa ttttaagtga
     8521 aaaatgattt tgattcatag agacctctgt aatatgcaag tgtgcaaatc gtccaaaggc
     8581 tctcacaact gttttgattt tttatattaa ttgaaaaaaa gtaattctca attaaattta
     8641 tagataggat tgtattccca ttttgacaaa aaacaaacta ctccttaccg tttatttcaa
     8701 aaaacgataa cattgtattg aaaaatatcc gaatttaaat acagaccgcc aatgcagaaa
     8761 aaaacaccca aattggctat aatcccgaca aacacactca aggacaacaa catggcagcc
     8821 tcgcccgaag caaaattcac cgaagaaaag attttgtggg tcaaacacca cacgccgaaa
     8881 ctcatcactt tcgccatcag ccgtcccgaa tcctaccgct ttaaagccgg acagttctcc
     8941 cgactcggtt tctacgaagg ggaaggtttc atttggcgtg cctattccat tgtttccgca
     9001 gaatatgccg acacgctcga atattttgcc gtactcatcc aagacggccc catgtctgcc
     9061 cgtttcgcca aaatgcaaca gggcgacacc atcctgctcg ataaaaatgc caccggcttc
     9121 ctgcttcccg aacgcttccc cgacggcaag gatttggtga tgctctgcac cggctccggc
     9181 atcgccccct tcctttccat tctcgaacaa cctgaaatcc ggcagcgttt cgataccgtc
     9241 aacctgatac attccgtatc ttttcccgaa gaattgattt tcaacgaccg gctcgccgca
     9301 ttgaccgaac atcccctggt agtcgaatac ggacactcgt tccgtttcct ccctgttacc
     9361 acccgtgccg ccaacccctc gggcttaagc ggaaaacgca ttccggaact cttaaaaaac
     9421 aacagcatcg aacaggcgct gcataccaag ttcaccccgg aatccacacg gtttatgatt
     9481 tgcggtaacc cggacatggt caaagacact ttccaaacgc tgcttgacat gggttacgcc
     9541 atgcaccgca accgcattcc cggtcaaatc atgatggaaa acggcttcta aaaaccaccc
     9601 tgcttgtccg atgccttcgg atggacggtc aaaccgacac ggcacgaaaa ccgcgtcggc
     9661 aaaaatgccg tctgaaaagt tcagacggca tcttcggaca cattacctgc aaacggtaac
     9721 acattccaac ccaaaccgat taggcaatca gtacgaacga ctgtttacat acttgccggc
     9781 tttcaccaac cggtatcgat ttaaccgatt tccttaatat ttttcctgtc cgttttaaac
     9841 ttcgccttaa acgcatccgg taaatcttta tcgaaatacc aaagcccgtc atccatttct
     9901 aatgcgccgc ccattccatg cagaacaact ttttcccccg ttaaaggatc ggtttcggta
     9961 atttccacct catgcatttt tccccaatcc aagacaggca gcttgtgtgc aaacgccaaa
    10021 acctgttcgt cagagcggac gcacgcctta tcacatcggt ctgaaacata tacgggacaa
    10081 ggctcatcct ctgtataacc gtccgacagg ataccggctg cggcaggaca caatccgtat
    10141 gtttccgccc acgacgacaa agcccgtctc gctgcctttt tattggcaaa taatccggta
    10201 ggcggattat ccgtcacacc gtttttcaaa gccgctgttt tcgcattcaa catgccgtct
    10261 gaaccttttt caaacctgac ggtcgtaaat gttttaagca gatttttggc agatacataa
    10321 caacccgaat gataacgtcc gaccaattcc gctttaatct tatatgcatg caggctgccc
    10381 aatgcaggaa aaaaacggac ttcctccgta ttgcaccaat caaacggggc ttttccggaa
    10441 tccaataaag ccgaaacctc gctatatacc cgttcaaacg taccgatgta atttactttt
    10501 cctccgccgt cgaaacaggc cagcaccccc ataccgtcag gcaaaccgta caactgttcc
    10561 ctcaaccgtt cgggcagcgc ggcaggcaac ggtttcggat tcatcaaacg gaaacactgc
    10621 ctgatccatg cctcgacccc gtgttccgac agactgtatt ccaaataatc gcacaatgcc
    10681 gatacatccg ccatcgcacg atgcctgtct tccacaacaa tccccaacct ttcgatgata
    10741 ctgtccaggc tgtgcttgta aaattgcgga tacagacacc gggacagctg cacactgcac
    10801 aaaacaggcg atgaaaatcc gatacccaca cgatgaaact catgctttaa aaacgtatag
    10861 tcgaaacggc tgttatgtgc aaccagcaca caacccttca ataccgaaaa caactcgccg
    10921 gcaatctctg caaaaacagg cgcatcggca accatgccgt ctgaaatccc cgtcagcccc
    10981 accacaaact gcggaatcgg tttttgagga ttaaccaacc actcatgcct caccaccctt
    11041 ccctgctcaa acttgaccaa agccacttcg gttaccctgt cttcatacag attgccgccc
    11101 gtcgattcca aatcaaccac ggcaacaggc attccaaacc gtaaaaatac cttttccagc
    11161 aagggccagc gagaagcaac aatcatttta ttctctttaa attcaaacaa caaaccaata
    11221 ttttacactt ttaaggcatt tcatccaaca aaacaattga cagaatccga tgattaccct
    11281 aaaattcgaa tctttcttgc ag
//
LOCUS       DAWGRZ010000051        10999 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000051 DAWGRZ010000000
VERSION     DAWGRZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10999
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.051"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(119..1336)
                     /locus_tag="WHT44_000954"
     CDS             complement(119..1336)
                     /locus_tag="WHT44_000954"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ2533155.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLATGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLTAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1536..2264)
                     /gene="rph"
                     /locus_tag="WHT44_000955"
     CDS             complement(1536..2264)
                     /gene="rph"
                     /locus_tag="WHT44_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ2533156.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMLREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2346..2810)
                     /locus_tag="WHT44_000956"
     CDS             complement(2346..2810)
                     /locus_tag="WHT44_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ2533157.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTAQELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3027..3803
                     /locus_tag="WHT44_000957"
     CDS             3027..3803
                     /locus_tag="WHT44_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ2533158.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            3941..4468
                     /locus_tag="WHT44_000958"
     CDS             3941..4468
                     /locus_tag="WHT44_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533159.1"
                     /translation="MEFVNNLVIFSFLLLMLIPIFFVVYGIYHKIRYRKICILRTSFI
                     LLVVILCSMYYIYCRYLDQQKVAYYCIDEQCISIVHLYKDYGINSPTYARIYAGKILF
                     RFQVRAKNYAELLMEDDISISKKILGNKFIIYGSLPVIYGNVDNIEVKEATGYIDRSS
                     TDYIVSRNLKFRHLY"
     gene            4822..5676
                     /locus_tag="WHT44_000959"
     CDS             4822..5676
                     /locus_tag="WHT44_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ2533160.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPKQGAAYVVVNFIALLELAKEGLVRIVQEDGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5717..6925
                     /gene="pncB"
                     /locus_tag="WHT44_000960"
     CDS             5717..6925
                     /gene="pncB"
                     /locus_tag="WHT44_000960"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ2533161.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIREDLEAELDALCQLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPGIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            7017..8735
                     /gene="argS"
                     /locus_tag="WHT44_000961"
     CDS             7017..8735
                     /gene="argS"
                     /locus_tag="WHT44_000961"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ2533162.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIADSPIVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVAEALADNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLAFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRIGRLKADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFIGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDANAPTVLTEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            9277..10695
                     /locus_tag="WHT44_000962"
     CDS             9277..10695
                     /locus_tag="WHT44_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ2533163.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLNFKIK"
BASE COUNT         2633 a         3101 c         2709 g         2556 t
ORIGIN      
        1 ttaatccact atattttttg ttccaaagtc aaaatatgcc gtccgaacat tcgggcggca
       61 gacaaaacgg cactgcccga taaaggcagt gccgttgtcc gtttcaaacc gtgaaacatc
      121 agcccaaatt aaaggcttta tgcaataccc tggttgccag ttccatgtat ttttcatcaa
      181 tcaatacgga aactttgatt tcggaggtgg aaatcatttg gatgttgata ccctcttcgg
      241 cgagcgtgcg gaagattttg gcggctacac cgacgtgcga acgcataccc aaaccgactg
      301 cggagacttt gcatacggtg tcgtcgccat caatagaagc cgcgccgata ctgtcttggc
      361 gttccgacag gatttccaaa gtctgcttgt aatcgccgcg cggtacggta aaggaaaaat
      421 cggttgtgcc ttcgctgccg acattttgga taatcatatc gacttcgatg ttggcatcgg
      481 caaccgcgcc taaaatctga taggcgacgc caggtttgtc gggtacgccg cgcacgttga
      541 tgcgggcttg gtttttatcg aatgcgatac cggttacggc agctctttcc atgttgtcgt
      601 cctcttcaaa ggtaattaag gtgccattgc cgccgtcttg caggctgctc agtacgcgca
      661 ggcgcacttt gtattttccg gcgaattcta ctgaacggat ttgcaaaact ttcgaaccga
      721 ggcttgccag ttcgatcatt tcttcaaatg taaccgtatc catgcggcgc gcttcgggta
      781 cgacgcgggg gtcggttgtg taaacgccgt ctacgtcggt atagatttgg cactcgtcgg
      841 ctttgagcgc ggcggcaagc gcgacggcgg aagtgtcgga accgccgcgt ccgagcgtgg
      901 aaatatcgcc ttcactgctg atgccttgga agccggcaac gatgacgact ttgccggcgg
      961 taaggtcggc acgcattttt tcgtcatcaa tgctttcgat gcgggctttg gtgtgggcgg
     1021 tatcggtttt gagggcgacc tgccagcctg tgtagctttt ggcatccacg ccgatgtctt
     1081 tcaatgccat cgccaaaagg ccgatggtta cttgttcgcc ggtagctaag acgacgtcca
     1141 gctcgcgcgg atcgggatgc tcttgcattt cgtgcgccag tgcgaccagt cggttggttt
     1201 cgccgctcat ggcggatacg acgactacga tgtcgtgtcc ttcggcgcgg gctttggcga
     1261 cacgtttggc tacgtttttg atgcgttcgg gcgagcctac tgatgtgccg ccgtatttat
     1321 gtacgattaa cgccatgttt cgtgctttct tgtgggggtt gtcgggcagc ttggtttgct
     1381 ggaaaaaggg ttattattac tattttttac atggaattca agaacggact gcgctttccc
     1441 gcctgccgtt tgacagcggt cagcgaaaaa cctgttcttt cagattgttg acaaaatgcc
     1501 gtctgaacgg ttttcagacg gcatccggac gacaatcagg cggcggacaa cgcattttgc
     1561 tggtgttgca gcagttcgcc tatgcctttt tgcgccagtg caaccagttt gcccaattcg
     1621 tccaaactga acggcgcgtc ttccgccgtc ccctgtattt cgatgatttt tcccgatgcg
     1681 gtcatgacga tattcacatc actgtcgcaa ccggagtctt cgggataatc caaatccaaa
     1741 agcggcacgc cgttcactac gcctactgac acagcggcaa cggcttcgcg gatggggttt
     1801 tcactcaaaa tgccgtctga aaccagtttg ccgacggcga tttgcagcgc gacaaacgca
     1861 ccggtaatcg aagccgtgcg cgtaccgccg tctgcctgaa tcacatcgca gtcaatcaag
     1921 atttgtcgtt caccgagttt ttccatatcc acgaccgcgc gcagggaacg cccgatcaaa
     1981 cgttggattt cttgtgtgcg cccggactgt ttgcccgccg aagcttcgcg gagcatccgg
     2041 gaagcagttg aggcaggcag catcccgtat tccgccgtta cccagccttg gtttttaccg
     2101 cgcagaaacg gcgggacgtt ttcatctatg gaagcggtac aaatcacttt ggtattgccg
     2161 cattcaataa ggcacgaacc gtccgtatgc ggcaggaaat gaggggtgat tttgatatcg
     2221 cgcaggctgt cggcggcgcg cgagatgcgg atgtaatcag gcatactgcc ctcccgttaa
     2281 aaacagataa attaaaaagc cttaaatatg aaaaatcaca tttaaggcct tcaaactgaa
     2341 aatttctacg cctcttcggc tttgctgcgg ataatcaaaa gcggcaggtg gctttggcgc
     2401 attaccgttt cggcaaaact gcccattaaa aggtgcatca gcccggtacg tccgtgcgta
     2461 cccaacacca gcaggtcggc accgttttca tcggcataat caaccaaatc ctgcgccatt
     2521 tcacgcgcac ccttattggc aaccagcagg tgtttgacgg tattttccac acccagttcc
     2581 tgggcggtgc gctcggcggc atccaaaact tcgttgcctt gcgcgacggc ggcggcttcg
     2641 tagctttcgt gttgcaaaaa ttcgggggcg agtgccatat attcggcagg attggcaacg
     2701 tgcaccaaag tcaggcgcgc accgttgacc ccggcaagct cggcggcatg tttcagggca
     2761 ttgatggacg tttcactgcc gtcaacggca acaaccaaat gtttgtacat atcgtattct
     2821 ccttttgcac cgcctcgcgg tgccctcttg tcggatgggc gcagggacag tttgcgctgt
     2881 ttcattatag acccgccgtc gggctttata caacagccga acagcccgac cgctttccag
     2941 tataatatgc cgcttccgtg cagtcaggca ttttttgccg gctttcgttc actttttgat
     3001 ttgacgcaat cttgcaggat tcgaccatgt ccgacaacgc tttgacctct tcgcgacgct
     3061 tcggcggcat cgccagactc tacggagact ctgccttggc gcacttttca caggcacacg
     3121 tctgcgtagt cggcgtgggc ggtgtcggct cgtgggcggt cgaggctttg gcgcggacgg
     3181 gcatcggacg tttgactttg attgatttgg acaacgttgc cgaatcgaat gtcaaccgcc
     3241 agctgcacgc cctgaccggc gacttcggca aagcaaaagt taccgccttg cgcgaacgca
     3301 ttacacaaat taatccgcaa tgcgaagtgt ttgaaattga agatttcgtt accgaagaca
     3361 atttgccgga atacttcgga aaaggttttg atttcgtcat cgacgcgatc gaccaagtgc
     3421 gcgtcaaagc agcaatggcg gcttattttg tggaacgcaa acaaccgttt gtcctcagcg
     3481 gcggcgcggg cggacaaaaa aatccggcgt taatccaaac cgccgatttg agccgcgtaa
     3541 cccacgaccc gctgcttgcc aacctgcgct acaccttgcg gaaacgctac ggattcagcc
     3601 gcgatacgaa agcaaatatg cgcgtgcctt gcgtgtattc gaccgaaaat atcgtgccgc
     3661 cgcagtctag ggaggcttgt tcggcagatg ccgctccgca aggcttgtcg tgcgccggct
     3721 acggtgcaag catgctcgtt accgcttcgt tcgggctata ttgcgcacag gcggcggtgg
     3781 aacacatcgc agacaaaaaa taagcaatgc cgtctgaaac aggattcaga cggcatttga
     3841 acaaactatg gttatgattt aagacaacaa aggatacgga taaaaaataa cataaaatat
     3901 atgattccta ataatatacc aagtatcgga gagctattta atggaattcg ttaataattt
     3961 agttattttt tcatttttat tactaatgct tattccgata ttttttgtag tatatggtat
     4021 ataccataag atacgttatc gcaaaatatg tatcctaaga acaagtttta tattattagt
     4081 ggtaatactt tgcagtatgt attacatata ttgccgttat cttgaccaac aaaaagtagc
     4141 ttattattgc atagatgaac aatgtatttc tattgttcat ctatacaaag attatggtat
     4201 aaactctccc acatatgcga gaatttacgc aggaaaaata ttgtttagat ttcaagtaag
     4261 agctaaaaat tacgctgaat tacttatgga agatgatata tcaattagta aaaaaatttt
     4321 ggggaataaa tttatcattt atgggtcgct acctgtaata tacggtaatg tagataatat
     4381 tgaagtaaaa gaagctactg gttatataga tagatccagt actgattata ttgtctcaag
     4441 aaacttaaaa ttcagacatt tatattaatt aagaggtttt agcaagagtg ccgtcaaaat
     4501 atagggcgca tcatcgaatt cgcgaaagac aaacgctacg atgaacgttt caaggatttg
     4561 aaaaaagaat ccataggcta tctgaaccgg catcccggtt tggtgtccga ctacctgaag
     4621 gcggcaatca agctgtcggt tcagaaaaac caacatcagc acgcctaaaa ccgtattcac
     4681 aacctgctcc ttttcaaaac atttgcattt aaaagccgtt ataatgccgt ctgaacatct
     4741 gcccgaccac attatacgtg aatgtcggca gattgttttc ttttgtaaac ttatattaaa
     4801 atccacttac cgattcacgc catgccgccc atccctgccc catctgcacc atccgagcac
     4861 actgtcgcat gggtattcgg ccaacccgtt accgatttgc cccaggattt gtttattccg
     4921 cccgatgcat tgaaagtcgt attgggcagc ttccaaggcc ctttggatct actgctgtat
     4981 ctgatccgca aacagaatat cgacgtactg gatattccga tggtgaagat taccgagcag
     5041 tatctgcact acatcgccca aatagaaacc tatcagtttg atttggcggc ggaatatctt
     5101 ttgatggcag caatgctgat tgaaatcaaa tcgcgcctgc tgctgccgcg taccgaaacc
     5161 gtcgaagacg aagaagccga cccgcgtgcc gagttggtgc gccgcctgct ggcttacgaa
     5221 cagatgaagc tggcggcgca gggtttggac gcgctgcccc gagccggacg ggatttcgcg
     5281 tgggcttacc tgccgctgga aattgccgtc gaagccaagc tgcccgaagt ctatattacc
     5341 gacttgacgc aagcgtggct gggtattttg tctcgggcaa aacacacgcg cagccacgaa
     5401 gtaatcaaag aaaccatctc cgtgcgcgcg caaatgacgg caatcctgcg ccgtttgaac
     5461 ggacacggaa tatgcaggtt tcacgacctg ttcaatccca aacagggcgc ggcttacgtg
     5521 gtcgtcaact tcatcgcact gttggagctt gccaaagaag gattggtcag aatcgtgcag
     5581 gaagacggtt tcggagaaat ccgaatcagc ctcaatcatg agggggcgca ttcagacggc
     5641 atttccggca cacgaggcgg gcgcgatgtg ttctaatacg ccccaagccg ccaccaaaaa
     5701 tcgggagaca cgccatatga ccggcatcat acattcgctg cttgacaccg acctctacaa
     5761 attcactatg ctgcaagtgg ttctgcacca gtttccgcag acgcacagcc tttacgaatt
     5821 ccgctgccgc aacgcctcga ccgtctatcc gcttgccgac atcagggaag acttggaagc
     5881 cgaactcgac gcgctctgcc aactacgctt cacccacgac gaactcggct atctgcgctc
     5941 cctgcgtttc attaaaagcg actttgtcga ttatctcgaa ctcttccagc tccaacgccg
     6001 ctttgtcgaa atcggcacag acgataaaga ccgtctgaac atccgcatcg aaggtccgat
     6061 gatacaggcg atgttttttg aaatcttcat cctcgccatt gtcaacgaac tttacttccg
     6121 ccgcctggaa acccctgcag tcatagaaga aggcgaacgc cggcttcaag ccaaagccgc
     6181 gcgcctcaaa gaaatcgccg ccgcacaaaa ccccgacgaa ccgcccttcc tgatttccga
     6241 cttcggcacg cgccgccgct acaagctcgc gtggcaggaa cacgtcatcc gcaccctgct
     6301 tgaagccgcc cccggcatcg tacgcggcac cagcaatgtc tttctcgcca aaaaactcgg
     6361 catcaccccc atcggcacca tggcgcacga gttcctgcag gcattccagg ccctcgacgt
     6421 acgcctgcgg aatttccaaa aggccgcgct cgaaagctgg gtgcacgaat accggggcga
     6481 tttgggcgtt gccctgaccg acgtggtcgg tatggatgcc ttcctgcgcg atttcgacct
     6541 ctatttcgcc aaacttttcg acgggctgcg ccacgacagc ggcgaccctt acgtttgggg
     6601 cgacaaagcc tacgcccact atcaaaagct caaaatcgac agccgcacca aaatgctgac
     6661 cttctccgac gggctggaca tcgaacgctc ttgggcattg caccaatatt tcaaagaccg
     6721 cttcaaaacc ggcttcggca tcggcaccaa cctcaccaac gatatggggc atacgccctt
     6781 gaatatcgtc ttgaaactgg tcgaatgcaa cgggcagtcc gtcgccaagc tgtccgactc
     6841 tccgggcaaa accatgacca acaacagcac cttcctcgcc tacctgcgcc aagtgttcga
     6901 cgtacccgaa cccgaaacgc cgtaaaccgg cagaaaaagc gcacaattcc tgtttctgcc
     6961 gcataaaatc ttttaaaata ccgcctgatt tgaatttaac cgaaagaccg aacttcatga
     7021 acctacatca aaccgtcgaa cacgaagccg ccgccgcctt tgccgccgca ggcatcgccg
     7081 acagccctat tgttttgcag ccgaccaaaa acgccgaaca cggcgatttc caaatcaacg
     7141 gcgtgatggg tgcggcgaaa aaagccaaac aaaacccgcg cgagttggcg caaaaggtcg
     7201 ccgaagcatt ggcggacaac gccgtgattg aaagcgcgga agtcgccggt ccgggcttca
     7261 tcaacctgcg cctgcgcccc gaatttctcg cgcaaaacat tcagacggcc ttgaacgacg
     7321 ctcgtttcgg cgtggcaaaa accgacaaac cgcaaaccgt cgttatcgac tattcttcgc
     7381 ccaatctggc gaaggaaatg cacgtcggcc acctgcgttc cagcatcatc ggcgacagca
     7441 tttcgcgcgt gttggcattt atgggcaata ccgttatccg tcaaaaccac gtcggcgact
     7501 ggggtacgca gttcggtatg ttggtcgctt atttggtcga gcagcaaaaa gacaatgccg
     7561 cgttcgagct ggcggatttg gagcagtttt accgcgccgc caaagtgcgc tttgacgaag
     7621 accctgcctt tgccgacacc gcacgcgaat acgttgtgaa gctgcaaggc ggcgatgaaa
     7681 ccgttttggc attgtggaaa cagtttgtcg atatttcgct ctcgcacgcc caagccgttt
     7741 acgacacgct gggcttgaag ctgcgtcctg aagacgtggc aggcgaatcg aaatacaacg
     7801 acgatttgca gcccgtggtc gatgatttgg ttcaaaaagg tctggcggtt gaggacgacg
     7861 gcgcgaaagt cgtgttcttg gacgaattta aaaacaaaga aggcgaaccc gccgcattta
     7921 tcgtgcaaaa acaaggcggc ggcttcctct acgcctccac cgatttggcg tgcctgcgct
     7981 accgcatagg ccgtctgaaa gccgaccgcc tgctgtacgt cgtcgaccac cgccaagccc
     8041 tgcacttcga acaacttttc accacttccc gcaaagcagg ctatctgccg gaaaacgtcg
     8101 gcgcggcatt tatcggcttc ggcaccatga tgggcaaaga cggcaagccg ttcaaaacgc
     8161 gcagcggcga caccgtgaaa ctggtcgatc tgctgaccga agccgtcgag cgcgccaccg
     8221 ctttggtgaa agaaaaaaat cccgaattgg gtgcggacga agccgctaaa atcggtaaaa
     8281 ccgtcggcat cggcgcagtc aaatacgccg acttgagcaa aaaccgcacc agcgactatg
     8341 tgttcgactg ggatgccatg ctctcgtttg aaggcaacac cgccccctat ctgcaatacg
     8401 cctacacccg cgtgcaaagc gtgttccgca aagcaggcga atgggatgca aatgcgccaa
     8461 ccgttttgac cgaaccgctg gaaaaacagc ttgccgccga gctgctgaaa tttgaagacg
     8521 tactgcaaag cgtggcggac acggcgtatc cgcactacct cgccgcctac ctctatcaaa
     8581 ttgcgaccct gttcagccgc ttctacgaag cctgtccgat actcaaagcc gaaggcgcaa
     8641 gccgcaacag ccgcctgcaa ctggcaaaac tcaccggcga cacgctgaaa caaggcttgg
     8701 atttgctggg catcgatgtg ttggacgtaa tgtaaaaccg caccgcccga ttgcggacaa
     8761 cagcctcgcc atccttatcc gaatctgaaa aaagcggcgc gatacaccgt atccgccgcc
     8821 cctcccaaaa tgcgaaacaa acaaacgcca agcaagcaag caagcaagca agcaagcaag
     8881 caagcaaaaa attataaccc ccttcctgcc gacgcacgca ctttccgcgc ggcgcattcc
     8941 ccttttcccg cccctcaaat ccgccttttc ttcaggcagg gtttcagccc gcctcttttc
     9001 cctgttttcc tttccccgac acgcgtgcgc tccccctgcc gcactgtgct gcactttcgc
     9061 gcccggacgg catcgttccg ccatccggtt ctctgtttta catacccctg tttcagaaag
     9121 aaatgcagat gtttcaacac acaggacgac acataaagca ccgccctatg tgttgccctg
     9181 atttggaagg ggttacgcct cccaaataaa gtctgatcct gccgccccga aggacagatg
     9241 tccgagtggc gaagtttcaa ccgaaaagga aatacgatga atattcacac cctgctctcc
     9301 aaacaatgga cgctgccgcc attcctgccg aaacggctgc tgctgtccct gctgatactg
     9361 cttgccccca atgcggtgtt ttgggttttg gcactgctga ccgccaccgc ccgcccgatt
     9421 gtcaatttgg actatcttcc cgccgcgctg ctgatcgccc tgccttggcg tttcgtcaaa
     9481 attgccggcg tattggcgtt ttggctggcg gttttgtttg acgggctgat gatggtgatc
     9541 caactcttcc cttttatgga tctcatcggc gccatcaacc tcgtcccctt catcctgacc
     9601 gcccccgccc cttatcagat aatgaccggg ctgttgctgc tgtatatgct ggcgatgccg
     9661 tttgtgttgc agaaagccgc cgccaaaacc gacttccggc acattgccgt ctgcgccgcc
     9721 gttgtggcgg cagccggcta tttcaccggc catttgagtt actacgaccg gggtcggatg
     9781 gccaatatct tcggcgcaaa caacttctac tacgccaaaa gtcaggcgat gctctacacc
     9841 gtcagccaga atgccgactt tattaccgcc ggcctggtcg atcccgtctt cctccccttg
     9901 ggcaatcaac agcgtgccgc cacgcatctg aacgagccga aatctcaaaa aatcctcttt
     9961 atcgtcgccg aatcttgggg gctgccggcc aatcccgaac ttcaaaacgc cacttttgcc
    10021 aaactgctgg cgcaaaaaga ccgtttttcg gtttgggaaa gcggcagttt tcccttcatc
    10081 ggcgcgacgg tcgaaggcga aatgcgcgaa ctgtgtgcct acggcggttt gcgcgggttc
    10141 gcactgcgcc gcgcgcccga cgaaaaattt gcccgctgcc tccccaaccg tttgaaacaa
    10201 gaaggttacg ccacctttgc gatgcacggc gcgggcagtt cgctttacga ccgcttcagc
    10261 tggtatccga gggcgggctt tcaagaaatc aaaaccgccg aaaacctgat cggtaaaaaa
    10321 acctgcgcca ttttcggcgg cgtgtgcgac agcgagctgt tcggcgaagt gtcggcattt
    10381 ttcaaaaaac acgacaaggg actgttttac tggatgacgc tgaccagcca cgccgactat
    10441 cccgaatccg acattttcaa ccacaggctc aaatgcaccg aatatggcct gcccgccgaa
    10501 accgacctct gccgcaattt cagcctgcac acccaattct tcgaccaact ggcggatttg
    10561 atccaacgcc ccgaaatgaa aggcacggaa gtcatcatcg tcggcgacca tccgccgccc
    10621 gtcggcaacc tcaatgaaac cttccgctac ctcaaacagg ggcacgtcgc ctggctgaac
    10681 ttcaaaatca aataacaaca atgccgtctg aacgcaccaa cagccttcag acggcatttt
    10741 gcagacagac cgacccttca agcccacttt tttcatcatc tccgataaat tgctttgtat
    10801 agtggattaa caaaaaccag tacggcgttg cctcgcctta gctcaaagag aacgattctc
    10861 taaggtactg aagcaccaag tgaatcggtt ccgtactatt tgtactgtct gcggcttcgt
    10921 cgccttgtcc tgatttttgt taatccgcca taaagaccgt cgggcatctg cagccgtcat
    10981 tcccgcgcag gcgggaatc
//
LOCUS       DAWGRZ010000052        10977 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000052 DAWGRZ010000000
VERSION     DAWGRZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10977
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.052"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(68..1624)
                     /gene="dnaA"
                     /locus_tag="WHT44_000963"
     CDS             complement(68..1624)
                     /gene="dnaA"
                     /locus_tag="WHT44_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ2533164.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     VSEGMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRQAAGSASRPESVA
                     VAKARTDVQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            1916..2050
                     /gene="rpmH"
                     /locus_tag="WHT44_000964"
     CDS             1916..2050
                     /gene="rpmH"
                     /locus_tag="WHT44_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ2533165.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            2053..2418
                     /locus_tag="WHT44_000965"
     CDS             2053..2418
                     /locus_tag="WHT44_000965"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ2533166.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLGH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRATAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            2483..2704
                     /gene="yidD"
                     /locus_tag="WHT44_000966"
     CDS             2483..2704
                     /gene="yidD"
                     /locus_tag="WHT44_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ2533167.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            2876..4513
                     /gene="yidC"
                     /locus_tag="WHT44_000967"
     CDS             2876..4513
                     /gene="yidC"
                     /locus_tag="WHT44_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ2533168.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGRQSVCAAGECNIDI
                     KRRNDKLYSTSVSVPLAAIQNGAKAEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(4594..5304)
                     /locus_tag="WHT44_000968"
                     /pseudo
     CDS             complement(4594..5304)
                     /locus_tag="WHT44_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356435.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            complement(5366..5956)
                     /gene="maf"
                     /locus_tag="WHT44_000969"
     CDS             complement(5366..5956)
                     /gene="maf"
                     /locus_tag="WHT44_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ2533169.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAIVLLNTVTGRMRRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCALKSEDL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            6104..6607
                     /locus_tag="WHT44_000970"
     CDS             6104..6607
                     /locus_tag="WHT44_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ2533170.1"
                     /translation="MSDPNLIDLEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            6641..6820
                     /gene="rpmF"
                     /locus_tag="WHT44_000971"
     CDS             6641..6820
                     /gene="rpmF"
                     /locus_tag="WHT44_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ2533171.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            6995..7522
                     /locus_tag="WHT44_000972"
     CDS             6995..7522
                     /locus_tag="WHT44_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ2533172.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLKELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            7611..8666
                     /gene="plsX"
                     /locus_tag="WHT44_000973"
     CDS             7611..8666
                     /gene="plsX"
                     /locus_tag="WHT44_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ2533173.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDEAPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENVGGL"
     gene            8802..9053
                     /locus_tag="WHT44_000974"
     CDS             8802..9053
                     /locus_tag="WHT44_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ2533174.1"
                     /translation="MKTLEKRMKALDKRIMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            9080..9298
                     /locus_tag="WHT44_000975"
                     /pseudo
     CDS             9080..9298
                     /locus_tag="WHT44_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9421..9624
                     /locus_tag="WHT44_000976"
     CDS             9421..9624
                     /locus_tag="WHT44_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533175.1"
                     /translation="MPSEAAWAFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PVSIMCTLSVLGNHLFFAYNSRI"
     gene            9651..10613
                     /locus_tag="WHT44_000977"
     CDS             9651..10613
                     /locus_tag="WHT44_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ2533176.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAAHRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDTPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            10660..>10977
                     /locus_tag="WHT44_000978"
     CDS             10660..>10977
                     /locus_tag="WHT44_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ2533177.1"
                     /translation="MKRQAFFKLMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRCAIRGQRRIPEHRLLLPALLGGWVGAYFGSMTFKHKTAKKRFVVLFRLTVSGNVLA
                     TLIL"
BASE COUNT         2807 a         3301 c         2563 g         2306 t
ORIGIN      
        1 ttaatccgct atatctttac ccttcggacg gcatgggcaa tatcatgtcg tctgaaaacg
       61 ttttccatca gttttgaatc agaatcagca gcttttcata atcctgagcc aattccggat
      121 cttcttcgcg cagtttcgcc actgccctga tgccgtgcat aacggtcgta tggtcgcgcc
      181 caccaaacga atcgccgata gacggcaggc tcaaagtagt cagttctttg gtcaggctca
      241 tcgccacctg gcgcggacgg gcaatgtttc gtgtccgttt cttaccgagc acatcgctga
      301 ttttgatgcg gtaatatttc gccaccgcat cgatgatgat gtcggcggta atgactttgt
      361 gcttctcggc aataatgtcc tgcaaagcgg tacgcgccaa atcgatgtcg atgacgggac
      421 ggttcataaa gcggctgctc gctccgacac gattaaacgc gccttcaagc tcgcgcacgt
      481 tggaacggat cagattggca atgaacagcg cggcttcgtc ttcgatactg atgcccgccg
      541 cttccgcctt tttctgcaaa atggcgatgc gcatttccaa ttcgggcggc tcgagttcca
      601 aagtcagtcc ccatgaaaaa cgggatttga ggcggtcgtc catgccttcg attttcgcag
      661 gcaacacatc gcaagtgagg atgagctgtt ttttctcgtt gtggaaatgg ttgtacagat
      721 agaaaaactc ttccatcgta cggtctttgc ctttgatgaa ctggatgtcg tcgataatca
      781 gcaggtcgta ttgcttgtat tgctgcttga acacgtcgta agtgttgttg cgaaccgcct
      841 tcataaagct gcggatatag tcatccgaat gcatatagcg cactttggca tcgggacggt
      901 ttttcagcag ctcgttgccg accgcctgca caaggtgggt tttgcccaaa cccgtgctgc
      961 catagaggaa gaacgggttg taactctgcc ccgggctttc cgcaatcgcc tgcgccgcag
     1021 ccgccgcaag gcggttgccc ttaccttcta ccaacgtatc aaacgtgtaa tccggagaca
     1081 ggttggtctg ctcgtaacgc gcctcttccg catcgcgctg cacgtccgtc cgtgctttgg
     1141 caactgccac cgattccggc cgggaagcag acccggcagc ctgacgcggc tcgtgcggca
     1201 ggtttttcat acgttccgcc aaaatatccg ccgccgtttt cgacgcagcg ggtttgacag
     1261 gctcttcaga cggcagctcg tccaacagaa cctcctgcac gggcattccc tccgacaccg
     1321 catgcaagga cggctcggca ggttcgacag caccttcaac cgccgccatc tcataacgca
     1381 cgccttctcc cggtttgaat acgaaggcgg aacggccggc agccaactct tccctcaccg
     1441 cttctatttt tccggcaaac tggctcttga gcatattgca ggcaaactgg ttcttgccgt
     1501 acaccaccca tacgccaccc tcctcaccaa cggtaagggg cgcaatccat tgcgcaaact
     1561 gcccttgagg caacatatcg tgaagacggc ggaggcacag cggccaaaac tctgctaatg
     1621 tcatggatag gctcgaatcg gtaaaaatga aatcgaaaac aaagaaaata taatattttc
     1681 aaaaagaaaa caaatctgtt gaacgcacat cggttcaaaa cgcgactgcc cgattatacc
     1741 gactcacgaa tattttatcc acaacccgtg caaaaattta tccacagaaa ggcggcggaa
     1801 atccgcaggc aatcgggcaa tcttcctgca aagtttctat attgattgac aaaagcggca
     1861 aattggagtg taattcacgg tttaattatc tacccattct attttaggaa acatcatgaa
     1921 acgcacttat caaccttccg ttaccaaacg caaacgcacc cacggcttcc tggtgcgctc
     1981 caaaacgcgc ggcggccgcg cagtattggc cgcacgccgt gccaaaggcc gcaaacgcct
     2041 ggcggtataa ttttggacta ccgcttcgga aggcagtacc gcttgttgaa aacggatgat
     2101 ttttcatccg tttttgcatt cagaaaccgc cgcagccgcg acctgctgca agtttcgcgc
     2161 tcaaacggca acgggctggg ccatccccgc atcggtctgg tggtcggcaa aaaaaccgcc
     2221 aaacgcgcca acgaacgaaa ttatatgaag cgcgttatcc gcgactggtt tagattgaac
     2281 aaaaaccggc tgccgccgca ggatttcgtc gtgcgcgtcc accgtaaatt cgacagggct
     2341 accgcaaaac aggcaagggc ggaactggca caactcatgt tcggcaaccc cgcaaccgga
     2401 tgcaggaaac aggcatgatc agaacggtac tctgcaggca aggttcagac ggcaacgggt
     2461 ttcccataca aggaacatcc cgatgaactt cctattgtcc aaactcctgc tgggactgat
     2521 acggttctac caatattgca tcagcccgct gattccgccg cgctgccgtt atacgccgac
     2581 ctgttcgcaa tacgcggtcg aagcggtcaa aaaatacggc gcattcaaag gcggccggct
     2641 cgccatcaag cgcattgcac gctgccaccc tttcggcgga cacggacacg accccgttcc
     2701 ctgacccgac gcaatattca aattgcacgc tttcctttta tttcccatcg gtttctatat
     2761 aatgccgtct gaagcttcgg gcaggcggca cgaccgccgg gtatgaagcc cgcccttatt
     2821 ccccgtctat cggaacacgc aacctgcggc atttccgacc attcaggaaa ctcttatgga
     2881 ttttaaaaga ctcacggcgt ttttcgccat cgcgctggtg attatgatcg gctgggaaaa
     2941 gatgttcccc actccgaagc ccgtccccgc gccccaacag gcagcacaac aacaggccgt
     3001 aaccgcttcc gccgaagccg cgctcgcgcc cgcaacgccg attaccgtaa cgaccgacac
     3061 ggttcaagcc gtcattgatg aaaaaagcgg cgacctgcgc cggctgaccc tgctcaaata
     3121 caaagcaacc ggcgacgaaa ataaaccgtt catcctgttt ggcgacggca aagaatacac
     3181 ctacgtcgcc caatccgaac ttttggacgc gcagggcaac aacattctaa aaggcatcgg
     3241 ctttagcgca ccgaaaaaac agtacagctt ggaaggcgac aaagttgaag tccgcctgag
     3301 cgcgcctgaa acacgcggtc tgaaaatcga caaagtttat actttcacca aaggcagcta
     3361 tctggtcaac gtccgcttcg acatcgccaa cggcagcggt caaaccgcca acctgagcgc
     3421 ggactaccgc atcgtccgcg accacagcga acccgagggt caaggttact ttacccactc
     3481 ttacgtcggc cctgttgttt atacccctga aggcaacttc caaaaagtca gcttttccga
     3541 cttggacgac gatgccaaat ccggcaaatc cgaggccgaa tacatccgca aaaccccgac
     3601 cggctggctc ggcatgattg aacaccactt catgtccacc tggattctcc aacctaaagg
     3661 cagacaaagc gtttgcgccg caggcgagtg caacatcgac atcaaacgcc gcaacgacaa
     3721 gctgtacagc accagcgtca gcgtgccttt agccgccatc caaaacggcg cgaaagccga
     3781 agcctccatc aacctctacg ccggcccgca gaccacatcc gtcatcgcaa acatcgccga
     3841 caacctgcaa ctggccaaag actacggcaa agtacactgg ttcgcctccc cgctcttctg
     3901 gctcctgaac caactgcaca acatcatcgg caactggggc tgggcgatta tcgttttaac
     3961 catcatcgtc aaagccgtac tgtatccatt gaccaacgcc tcttaccgct ctatggcgaa
     4021 aatgcgtgcc gccgcaccca aactgcaagc catcaaagag aaatacggcg acgaccgtat
     4081 ggcgcaacaa caggcgatga tgcagcttta cacagacgag aaaatcaacc cgctgggcgg
     4141 ctgcctgcct atgctgttgc aaatccccgt cttcatcgga ttgtattggg cattgttcgc
     4201 ctccgtagaa ttgcgccagg caccttggct gggttggatt accgacctca gccgcgccga
     4261 cccctactac atcctgccca tcattatggc ggcaacgatg ttcgcccaaa cttatctgaa
     4321 cccgccgccg accgacccga tgcaggcgaa aatgatgaaa atcatgccgt tggttttctc
     4381 cgtcatgttc ttcttcttcc ctgccggtct ggtattgtac tgggtagtca acaacctcct
     4441 gaccatcgcc cagcaatggc acatcaaccg cagcatcgaa aaacaacgcg cccaaggcga
     4501 agtcgtttcc taaatgccgc agcatgaaaa atgccgtctg aaacctgttc agacggcatt
     4561 tttattgccc accccctatc ggggcggaaa tcttcaaccc gcatacatca caaaaatcgt
     4621 cgggcgtttt ttcagattgg gcatttcttt tctttttcgc cactgcacga ttgtttgact
     4681 gatgatttcc tgtgtcggca aggtcaaatc cgtagccgtg cataaacgcg tttcaggatg
     4741 caggttttcc accgcatcgg caagcagcgc atcattgcgg taaggcgttt caataaaaat
     4801 ctacgtctcg ccgcactggc gcgaacgctg ttccaaagcc cgaaaagcct gaatccgctc
     4861 gtttttttca gacggcagat agcctttaaa cgcaaaactc tgcccgttcg cacccgaagc
     4921 catcaaagcc agcagcaggc tggaaggccc gaccagcgga cgcacttcaa aaccgtgttt
     4981 atgcgccaat gccaccaaat tcgcacccgg atcggccaca gccgggcaac ccgcctcact
     5041 gacaatgccc atactgcgcc cttcttgcaa aggtttcagc aattccggca aagtcttcaa
     5101 atccgtatgt tcattcaacg tttgcagatt cagctcgcgg ataggcgtag tcacgcccaa
     5161 atgtttcaaa tgcgcacgcg ccgttttttc cgcctccacg acaaaatccg tcagcccgac
     5221 aatcgcctgt tgttcatgcg gcaacaggca cggcgtgtca ggcgtaccca aaggcgtagg
     5281 aatcaaatac aaaacaggag acatcattcc ctcactcatc ggttaaaaat gccgtctgag
     5341 cctttcagac ggcataaacg ggcagttaca gaacctccac gccctcattt ttcaagaaat
     5401 cgaccagacg gaaaaccggc aaaccgatta aagcattcgg atcggtactc tcaatccttt
     5461 caatcagcaa tgcacccaaa tcctcactct tcagcgcaca cgaacaataa accgcatcag
     5521 gctcgcgctc caaatagcgg aggatatgca actcgtccaa ctgcctcatc acgaccaccg
     5581 tcttatcgat atgccgccgc atcctgcccg taaccgtatt caacagcacg atcgcgctgt
     5641 aaaactcaat ctccctgccg ctcaagtgca tcagcatctt ttgcgcattg gcaaggttca
     5701 tcggcttgcc ccactgcctg ccgtcgcacc acgccacctg gtccgcaccg acaatcaacg
     5761 cctctgggaa acgcccggtc aacgaccgcg ccttaccctc ggcaaggcgc aatgccgtct
     5821 gaggggcgga ttcccccaac atcggcgttt cgtcaaaatc gggggacgcc gcctgaaagg
     5881 caatgccgag cctttccatc tgttcgcggc ggaaaaccga actcgtaccc aaaatcaaag
     5941 gcagttccaa acccatccca tcctccttac cgttgaaaac acgcccgaag gggcagtaaa
     6001 atccagccat gcgccgaaac acggataccc gccttcggcg taccgcaaca tttttcttaa
     6061 aaatattgac gttagaacat ctaaattata tcatatcccg tttatgtcag accctaattt
     6121 gattgacttg gaaatttttg ccgccgaagg gcagaacctg caaggcagtt ttctgctgga
     6181 agaattggat gaacgcgtca gttcgcacga ttatcccgcc gacaggcaga ccaaaatatc
     6241 gtttacactg accggcggtc gcgaccggct gcaacgcctg ttcctcgacc tgaacgtcaa
     6301 agccgatatg cccctgattt gccagagatg tatcaaaccc atgccgttca tgcttgatga
     6361 aagcagccgt atcgtcctgt tttccaacga agagtccttg gacgaatcca tgcttgccga
     6421 cgaagaactc gaaggcatac tgattgaaaa agaactcgac gtgcgcacat tggtagaaga
     6481 ccaaatcctg atgtccctgc ccttttcgcc gcgacacgaa gactgcggcg acaatgggac
     6541 actggaagaa gtcaatcggg acaaacccaa cccctttgct gttttggcag gtttgaaaag
     6601 caattgatta ggacacagtt tatttatcta ggagcttgaa atggccgttc aacaaaacaa
     6661 aaaatcccct tccaaacgcg gtatgcaccg ttcgcacgac gcgctgaccg cgcctgcact
     6721 gtctgtcgac agcacaaccg gcgaagtaca ccgcccgcac cacatctccc ccaacggtat
     6781 gtaccgcggc cgcaaagtgg tcaaagccaa aggcgaataa tccctattcg actgactgaa
     6841 aaagccagaa cattgccatg caattactgg ctttttttgc attggacgca ccatccgtcc
     6901 aaactttcgc catacgtcaa cacacagggg caaagcgttc cgtataatac cccgtgaaaa
     6961 tattccaaaa gccccaacca ccaaggaaat tccgatgaaa cagaaaatct ggtacaccta
     7021 cgatgacatc caccgcgtca tcaaagcatt ggcagaaaaa atccggaacg ccgacatcaa
     7081 atacgatgcc atgattgcca tcggcggcgg cggctttatt ccggcacgta tgctgcgctg
     7141 ttttctggaa attccgattt atgccgtaac caccgcctat tacgacagcg acaacgaagg
     7201 acaggttacc gaagaagtca aaaaagtcca atggctcgac cccgttcccg aagccctgcg
     7261 gggcaaaaac gtactcgtcg tcgatgaagt ggacgacagc cgcgtaacca tggagttctg
     7321 cctgaaagaa ctgctcaagg aagacttcgg tacgatcgga gtcgccgtac tgcacgaaaa
     7381 aatcaaagcc aaagcaggca aaatccccga aggcattccc tatttcagcg gcatcaccgt
     7441 agaagactgg tggatcaact atccgtggga cgcactcgac atcgacgaac acaaccgcct
     7501 tgccgaggcc ggccgaggct gaccctttca gacggcatat tttccgaacc gatgccgtct
     7561 gaagcccgca cgacccctgc cgcagaccga aaacctaccg gagaaaccct atgattacat
     7621 tggccgtaga tgccatgggc ggcgaccaag gacttgccgt taccgtaccc ggcgcaaccg
     7681 cattcctcca agcacacccc gatgtccgcc tgattatgac cggcgacgaa acgcaactgc
     7741 gccaagccct gaccgcggca ggcgcaccga tggaacgcat cgacatctgc cataccaccc
     7801 aagtcgtcgg catggacgaa gccccgcaat ccgccctgaa aaacaaaaaa gactcctcca
     7861 tgcgcgtcgc catcaaccag gttaaagaag gcaaagccca agccgccgta tccgcaggca
     7921 acacgggtgc gctcatggca accgcacgtt tcgtcctcaa aaccattccc ggcatcgaac
     7981 gccccgccat cgccaaattc cttccttccg acaccgacca cgttaccctt gcactcgacc
     8041 ttggcgcgaa cgtcgactgc acgcccgaac agctcgccca atttgccgtt atcggcagcg
     8101 aactcgtcca cgcactccat cctcaaaaag gacagccgcg cgtcgggctg gtcaacgtcg
     8161 gcacggaaga catcaaaggt acagacaccg tcaaacaaac ctacaaactg ctgcaaaaca
     8221 gcaaactcaa ctttatcggc aacatcgaaa gcaacggcat cctctacggc gaagcagatg
     8281 tcgtcgtcgc cgacggcttt gtcggcaacg tcatgctcaa aaccatcgaa ggcgcggtca
     8341 aattcatgag cggagccatc cgccgcgaat tccaaagcaa cctgttcaac aaacttgccg
     8401 ccgttgccgc cctacccgcc ctcaaagggc tgaaaaacaa actcgacccg cgcaaattca
     8461 acggggccat cctgctcggg ctgcgcggca tcgtgattaa aagccacggc ggcacagacg
     8521 aaaccggttt ccgctatgcc ctcgaagaag cctaccacga agccaagtcc gccggccttt
     8581 ccaaaatcga acagggcgta gccgaacaac tcgccgcact cgaaactgcc aaagccgtcc
     8641 aaaacgaaaa tgtcggcggt ctgtaacaca cacgatgccg tctgaacgcc cccgcccctt
     8701 tcagacggca tccgcccgca ccaaacctgc gggcgcggac ggcgatgcgc ctgtccggca
     8761 cttcccaaat atcgccttgt aaaataagga gtatttgaaa aatgaagaca ttagaaaaac
     8821 ggatgaaagc tctagacaaa cggattatga agttcggaaa atcccttgaa ggcaggcttg
     8881 atgcccgtct gattgaatcc gcattggatt atattcatta ttcggaacgt tttttggctt
     8941 ttgaaatcct gtgtacttat atcgaagatt tcgatgtccg gctgacggaa caagaatccc
     9001 gggaaatttc ttttatcaac aaggaatttg agatagaaag cacgtccgat taaccaataa
     9061 agccaatggg ttgataaaca tgaaaacatc gacggtcgtt tttggcggat tttttatggc
     9121 agacaacgga gagcgaatcc aaatccccgt tttggaaaat cctgacatta gggaaatcaa
     9181 tcactttttt tccgtatcaa attttgagaa aaaaaccggc gtccttgttt tcagaatcat
     9241 ccccgagccg gaatttggca ataccgaatt aactgtctat tttaaaaaag gatattatag
     9301 tggattaaca aaaaccagta cggcgttgcc tcgccttgcc gtactggttt ttgttaatcc
     9361 actatatcag acgaaaacaa acacccgcgc caatagcctg acggcaaccc ggcaatcaaa
     9421 atgccgtctg aagcagcttg ggctttcaga cggcatttcc ttcgcttaaa acagcgtatc
     9481 ggcaaccccg ccctgcctgt ccacggcaat ctgcatctga aaaccatctg tatcccaaac
     9541 cacaccccca tccctgtttc catcatgtgc accctgtccg tattgggcaa tcatctgttt
     9601 ttcgcttaca atagccgaat ctgaaccaac tctctaaaaa ggccgttccc atgcagtatg
     9661 caaaaatttc cggcacaggc agctatcttc ccgccaaccg tgtcagcaat gacgaccttg
     9721 cccaaaaggt agatacctct gacgagtgga ttaccgcgcg cacgggcatc aaattccgcc
     9781 atattgcagc cgaaaacgaa aaaaccagcg atcttgccgc cgaagcggcg caccgcgcgc
     9841 tggatgcagc cggattagac agcggcgaaa tcgatttgat tatcgtggca acggcaacgc
     9901 cggatatgca gtttccgtct actgcgacca tcgtgcaaca aaaattgggc atcaccaacg
     9961 gctgccccgc gtttgacgta caggcggtgt gcgccggctt tatgtacgcg ctgaccacgg
    10021 caaacgccta cattaaaagc ggtatggcga aaaacgcgct ggtcatcggc gcggaaacct
    10081 tcagccgcat tgtagactgg aacgaccgca caacctgcgt attgttcggc gacggcgcgg
    10141 gcgcggtggt tttaagcgcg tcggacacgc cgggcatcat ccacagcaaa ctcaaggccg
    10201 acggcaatta tctgaaactc ttaaacgtcc ccgggcaaat cgcctgcggc aaagtttccg
    10261 gttcgccgta catttcgatg gacggtcccg gcgtgttcaa gtttgccgtc aaaatgctgt
    10321 ccaaaatcgc cgatgacgtt atcgaagaag caggttacac cgccgctcaa atcgactgga
    10381 ttgttcccca tcaggcaaac cgccgcatta tcgaatcgac cgcgaaacat ttaggtttga
    10441 gtatggacaa agtcgtcctg accgtccaag accacggcaa cacatccgcc gcatcgattc
    10501 cgctggcttt ggatacgggc atccgcagcg gacaaatcaa acgcggtcaa aacctgctgc
    10561 tcgaaggcat cggcggcggt ttcgcgtggg gcgcggtgct gttgcaatat tgaacccgat
    10621 gccgtctgaa acaggctttc agacggcatt tcccatatca tgaagcggca ggctttcttc
    10681 aaactgatgg cgtgtgcggc atttctgtct gccgtttcgc tgcgcctccc cgtattgggc
    10741 gcgtgttacg caatattgtc cctctatgcg tttgcacttt acggcatcga caaacggtgc
    10801 gccatacggg ggcaacgccg cattcccgaa caccgcctgc tgctgcctgc attgctcggc
    10861 ggctgggtgg gcgcgtattt cggcagcatg acattcaaac ataagacagc gaaaaagcgt
    10921 tttgttgtgc tgttccgtct gactgtttca ggtaatgtct tggcgaccct catcctg
//
LOCUS       DAWGRZ010000053        10891 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000053 DAWGRZ010000000
VERSION     DAWGRZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10891
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.053"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(69..452)
                     /locus_tag="WHT44_000979"
     CDS             complement(69..452)
                     /locus_tag="WHT44_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEZ2533178.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGREQELVLIW
                     RTEADIGNGSILQFVCNWGFPAAEKTCSVLKKIGAVHSAMLIHRAADALDKEIRRLQS
                     EGKNLKEMWDITSRQQNRLTAEQSG"
     gene            complement(510..1886)
                     /gene="argH"
                     /locus_tag="WHT44_000980"
     CDS             complement(510..1886)
                     /gene="argH"
                     /locus_tag="WHT44_000980"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ2533179.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRCRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(1905..2774)
                     /gene="galU"
                     /locus_tag="WHT44_000981"
     CDS             complement(1905..2774)
                     /gene="galU"
                     /locus_tag="WHT44_000981"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ2533180.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     NLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(2802..3401)
                     /gene="rdgB"
                     /locus_tag="WHT44_000982"
     CDS             complement(2802..3401)
                     /gene="rdgB"
                     /locus_tag="WHT44_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ2533181.1"
                     /translation="MSEKPEKIVLASGNAGKLEEFGNLFKPYSITVLPQSAFGIPECP
                     EPYPTFVENALAKARHAAKYSGLPALADDSGICAAALNGAPGIHSARYAGDNPKSDTA
                     NNLKLAAELVGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALAELLRKLAL"
     gene            complement(3394..3627)
                     /locus_tag="WHT44_000983"
     CDS             complement(3394..3627)
                     /locus_tag="WHT44_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533182.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGSLALYLRHKSLRAAQSATKENRYV"
     gene            complement(3735..4268)
                     /locus_tag="WHT44_000984"
     CDS             complement(3735..4268)
                     /locus_tag="WHT44_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ2533183.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(4369..4827)
                     /gene="nudB"
                     /locus_tag="WHT44_000985"
     CDS             complement(4369..4827)
                     /gene="nudB"
                     /locus_tag="WHT44_000985"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ2533184.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPEGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPIALQPEEHVSYGWFDMEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            5038..5114
                     /locus_tag="WHT44_000986"
     tRNA            5038..5114
                     /locus_tag="WHT44_000986"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5072..5074,aa:Pro,seq:ggg)
     gene            5272..7098
                     /gene="mafB"
                     /locus_tag="WHT44_000987"
     CDS             5272..7098
                     /gene="mafB"
                     /locus_tag="WHT44_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ2533185.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGKIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNINSNFSQHAQLEREINKLKSADEI
                     NFADGMGKFTDSMNDKAFSRLVKSVKENGFTNPVVEYVEINGKAYIVRGNNRVFAAEY
                     LGRIHELKFKKVDFPVPNTSWKNPTDVLNESGNVKRPRYRSK"
     gene            7101..7565
                     /locus_tag="WHT44_000988"
     CDS             7101..7565
                     /locus_tag="WHT44_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533186.1"
                     /translation="MAIWNYRYYLIPSAAIRNKFNANKDIILDEYRSNGFQNFNENKS
                     FENYFIDNDVILLSIINEAKKQLKLKESWDKDAIMFCDNFGNSLTVWPDDIECELDLR
                     FDYTKFIQKTIDWAIKYNCLLVIEKTGNVVSPNINNLMYEIKAYLESKPWPI"
     gene            7754..7936
                     /locus_tag="WHT44_000989"
                     /pseudo
     CDS             7754..7936
                     /locus_tag="WHT44_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223961.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
     gene            7948..8235
                     /locus_tag="WHT44_000990"
     CDS             7948..8235
                     /locus_tag="WHT44_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEZ2533187.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNQLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
     gene            8731..9987
                     /locus_tag="WHT44_000991"
     CDS             8731..9987
                     /locus_tag="WHT44_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ2533188.1"
                     /translation="MKSKSGVIASLTGAAVGGTPVDAQTGGAVGQNAVENNLYLTSEA
                     LKKDKQTARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDSRFKDLKKE
                     SLYYLNKHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGS
                     CAETFGLGCAAALVGVTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEY
                     VQFSIDLFSVGKSGGGIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQS
                     SQFKAHAQREWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGV
                     YQATVEIKKPDGSWEVKTKKGGKVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNK
                     WQGTSKSGIKIEGFTEPNRTAYPIYE"
     gene            10018..10437
                     /locus_tag="WHT44_000992"
     CDS             10018..10437
                     /locus_tag="WHT44_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533189.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            10628..10852
                     /locus_tag="WHT44_000993"
     CDS             10628..10852
                     /locus_tag="WHT44_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ2533190.1"
                     /translation="MRLATKDLAEAIRKGQVRKSSFNTEQLRAIEKGESKIPDYTWHH
                     HQDTGRMQLIREGLHHDTGHIGWEAMNKGR"
BASE COUNT         2849 a         2360 c         2919 g         2763 t
ORIGIN      
        1 gaacagggtt gctggaatag tattgccatc ccagcagata cagtttgtcg gggtcttgcc
       61 aatattgttc atccagactg ttcagcagtg aggcggtttt gttgtcgaga tgttatatcc
      121 cacatttctt tcaggttttt accttccgat tggaggcggc ggatttcttt gtccaatgcg
      181 tctgccgccc gatggatcag cattgcgctg tgtaccgcgc cgattttttt cagaacggaa
      241 caagtctttt cggcggcggg gaaaccccag ttgcagacaa attgcagtat gctgccgtta
      301 ccgatatcgg cttctgtccg ccaaatcaaa accagctcct gttcccgccc gtccatgctt
      361 tcgagtttgc cgtcatgctg ttcaaaaagt ttgtccaccg cctgccacat catctgttcg
      421 aagcggtcgg cttgggtatc cgtatcggtc atcgtgttct tgccttttaa aaatgccgtc
      481 tgaacatttc ttcagacggc atttgggggt taagccaaca tttcccgcca gcgtttcact
      541 tggaagcgga cttgttccgg cgcggtaccg cccaagtggt tgcgggcgtt taagctgcct
      601 tcgggtgtca gcacgccgta aacgtcgtcg gcaatcaaat cgctgaaacc ttgtaagact
      661 tcgagcggca gttcgctcaa atcgacgccc gcttggtcgg cgtggcgcac ggcttgggcg
      721 acgacttcgt gggcatcgcg gaaaggcatg ccttttttga ccagataatc cgccaagtcg
      781 gtggcggtag cgaagccctg catcacggcg gcgcgcatat tgtcgggttt gacggttacg
      841 ccgcgcatca tatcggcgta aatccgcaac gtgtcgataa gcgtgtcggc ggtgtcgaac
      901 aagggttctt tgtcttcctg attgtctttg ttgtacgcca agggttggga tttcatcagg
      961 gtaatcagac cgataaggtg tccgatgacg cggccggatt tgccgcgcac gagttcgggc
     1021 acgtcggggt ttttcttctg cggcatgatg gacgaacctg tgcagaaacg gtcggcgatg
     1081 tcgataaagc cgaaacgcgg gctcatccac aaaatcaatt cttcagacag gcggctcagg
     1141 tgaaccataa ccagcgaggc ggcggctgtg aactcaatgg cgaaatcgcg gtcggatacg
     1201 gcatcgagcg agttctggca gatttgttca aagcccaata gctcggcggt gatttcgcgc
     1261 tgaatcgggt aggtcgtccc ggcaagggcg gctgcgccga gcggcatacg gttgacgcgg
     1321 cagcggcagt ccgccatccg ttcgttatcg cgtccgagca tttcgacgta ggcgagcata
     1381 tggtgtccga agctgacggg ctgggcgact tgcaggtggg taaagcctgg catgacggtt
     1441 tcggcgtttt gttccgccaa atccagcaat gccgtctgaa ggctttgaat caggctttgt
     1501 ataacggtaa tctggtcgcg cagccacagg cggatgtcgg tggcgacttg gtcgttgcgg
     1561 ctgcggccgg tgtgcaggcg tttgcccgcg tcgccgattt tgtcggtcag gcggcgttcg
     1621 atgttcatat ggacatcttc caaatcggac gaccattcga ttttgccgct gcggatttct
     1681 tcgaggattt ccgccatacc ccggcggatg tccgccaaat cgccttcgtc caacacgccg
     1741 gtttctttca gcatttgcgc gtgtgccagc gagccttgga tgtcccattc ggcaagccgt
     1801 cggtcgaaac cgatggaggc ggtgtattgt ttgacgagtt cggaaacggg ttcgttgaaa
     1861 cgtccggacc aggttttgtc gtgcataagg attccttgat ggggttattc ggtgcggtat
     1921 ttttccaaaa gccggcggaa gggttcgccg gtttcgggat gtttcagacc gtaggcgacg
     1981 gtggcttcga ggtagcccag tttgctgccg cagtcgtagc gcgtaccttc aaaggggtgc
     2041 gccaggacaa attcgtgatc gagcagcttg gcgatgccgt ctgtaagctg gatttcgttg
     2101 cccgcgccgc gcggaagatt ggttaagagg tcgaaaatgc gcggggtgag gatgtagcgt
     2161 ccaacaacgg caaggttgga gggcgcgtct tcgggcttgg gtttttcgac aatgccggta
     2221 atgcgttgga actgtttgag ctgttcggtt tcgacgatgc cgtatgagcc ggtttgcgat
     2281 gcttcaacgg tttctacgcc caaaatgctg ttgccgctgc gcccgtacac ttcgaccatt
     2341 tgtttgagcg cgcctttggg ggcatcaatc aggtcgtcgg caaggataac ggcaaagggt
     2401 tcgtctccga tggcggcgcg ggcgcacaag acggcgtgtc ccaagcccag tgcttccgcc
     2461 tgacggatgt agaggcaggt aatgttcggc ggcaggatgt tgcggacgtg ttccaacaat
     2521 ttgtctttat ggcgcatttc caactcggtt tcgagttcgt atgccttgtc gaaatggtct
     2581 tcgatgctgc gtttgttgcg tccggtaaca aacaccattt ccgtgcagcc ggcttccacg
     2641 gcttcttcta cggcgtattg gatcagcggc ttgtcgacga tgggcagcat ttctttcggg
     2701 ctggccttgg tggcgggcag gaagcgggtt cccatccctg cgacggggaa aacggctttc
     2761 cgtatgggtt tcattctttt tcctttgtat tgttttgatg tttaaagggc gagtttgcgt
     2821 aagagttcgg caagtgcctg cgcgcggtgg ctttcgcggt ttttgacctc cgaatccaat
     2881 tcggcggcgg ttttgccgtg tccgggcaga taaaaatacg ggtcgtaacc gaaaccgttt
     2941 tcaccggcgg gaacgccgtt ccattgtccg tgccatacgc cttcggcaat aatcggacgc
     3001 gggtcgtctt tatggcggac aaaaaccaat acgcagacat agcagcagct tttgtctgcc
     3061 ttgccgacaa gttcggcggc aagtttcagg ttgttggcgg tatcggattt gggattgtcg
     3121 cccgcgtaac gtgcggaatg gatgcccggc gcgccgttta aggcggcggc acagatgccg
     3181 ctgtcgtcgg cgagtgcggg cagcccgctg tatttggcgg catggcgtgc ttttgccagc
     3241 gcgttttcga caaaggtggg atagggttcg gggcattcgg gtatgccgaa tgcggattgc
     3301 ggcaatacgg tgatgctgta aggtttgaat aagttgccga actcttcgag cttgccggca
     3361 ttgccgcttg ccaaaacgat tttttccggt ttttcagaca tagcggtttt cctttgtggc
     3421 ggattgggcg gcgcgtaggg atttgtgccg caggtagagg gcgaggctgc cgatttgtcc
     3481 gaacagtgcg ccgaatgcga aaagaaagga ggcgacggcg aaggctttgc tgccgacggt
     3541 cagcagataa gcacccaaga ggacgagcag cataaatgcc agtgtgaaga gggcaacaga
     3601 caacagatag atttttcggc ggttcatggc gttcggtcgg aaacggtatg ttcggattat
     3661 agccgattgg gacggtattc cctagagctt ggaaaaatgc cgtctgaaac ggcgttcaga
     3721 cggcatggcg ggcgttatgc ctgtttgttc caacgttcga tggattcttt gatgactttt
     3781 ttcgcttctt ccgcatcgcc ccacgattcg actttggtcg tacctgcttt tttcaggtct
     3841 ttgtaatggt tgaagtggaa ctcgatttgt ttgatgagct gttgcggcaa atcggacaaa
     3901 gttttgtagg cgttgccgtt gttgcggtcg tcggcaggaa cgcagacgat tttgtcgtcc
     3961 acttcgccgt cgtcaacgaa tttcatcacg ccgataacgc gcgcttccaa gaatacgccg
     4021 gttgccaaag gttgttcggt aacgagcagc acgtccaatt cgtcgccgtc ttcgtccaaa
     4081 gtttggggaa tgaagccgta gttggtcggt ttggcgaaga tggcgggttc gacgcggtcg
     4141 agttggaatg cggccagttt gcggttccat tcgattttgt ggttgctgcc ggcggggatt
     4201 tcgttgacaa cgttgatgat gccgccgtcc acgtcgccgg gggtcaggat ttggttgaag
     4261 tctgccattt ggtttccttt gtttgggaaa gtgtgaagtt tgaaagtata gcacaaacgt
     4321 ccggctgaaa atgcgcccga tgcctctgaa agggtgtacg ggcgcgtgtt accgtttgcc
     4381 caaaaacctg cccagttcca aaatcgcgcg cctgttggac ggggaaaata ctttttccgc
     4441 tgcttcttcc atatcgaacc aaccgtagga gacgtgttct tcgggttgca gggcgatggg
     4501 cgtatcacgc gggatttcgg cagagaagag gtgttcgcgg ttttcaaaca cgccttttgg
     4561 atagcggtgc cgccagtggt ggtagatttc gtaaaccgtg ctgtcgtgcc agtcttgaag
     4621 ctgcccgtcc gccagcagga tgccggtttc ttcccaaact tcgcgccttg ccgtttgggc
     4681 gacggtttcg cccggttcga ggctgccggt taccgactgc caaaatcctt ccggatgcgt
     4741 gcgttcgatg agcaggatgc cgccgtcccc gctataaagg acgaccagtg cggaaacggg
     4801 gtatttgagc ggttttgcca tcggcatctt tcggcgggct gcggtaatga aggggcggat
     4861 tatagcaaac gccgcacgtt atggcgttta tccttttccg tatccttttt tctgcacgga
     4921 tgggacgcgc cggtgtttgc cggtaaattt tccgttgtgt caaaaagata agggcggttg
     4981 tgattttaat gcttgccaaa gcgtcgggcg gaaactataa tccgaaactt atcgagtcgg
     5041 agtgtggcgc agtctggtag cgcacttgca tggggtgcaa ggggtcgaag gttcgaatcc
     5101 tttcactccg accaaaaatt ccgaaagccg ctttcaaaag cggctttttt gccgtccgta
     5161 tgattatgat gtagagtacg cggcgacaga cattcaaatg ccgtctgaaa accgttcaga
     5221 tggcatctct ttatcttagt ttcattccgt accatcttaa ggaacatcaa attgggcatt
     5281 tcccgcaaaa tatcccttat tctgtccata ctggcagtgt gcctgccgat gcatgcacac
     5341 gcctcagatt tggcaaacga ttcttttatc cggcaggttc tcgaccgtca gcatttcgaa
     5401 cccgacggga aataccacct attcggcagc aggggggaac ttgccgagcg cagcggccat
     5461 atcggattgg gaaaaataca aagccatcag ttgggcaacc tgatgattca acaggcggcc
     5521 attaaaggaa atatcggcta cattgtccgc ttttccgatc acgggcacga agtccattcc
     5581 cccttcgaca accatgcctc acattccgat tctgatgaag ccggtagtcc cgttgacgga
     5641 tttagccttt accgcatcca ttgggacgga tacgaacacc atcccgccga cggctatgac
     5701 gggccacagg gcggcggcta tcccgctccc aaaggcgcga gggatatata cagctacgac
     5761 ataaaaggcg ttgcccaaaa tatccgcctc aacctgaccg acaaccgcag caccggacaa
     5821 cggcttgccg accgtttcca caatgccggt agtatgctga cgcaaggagt aggcgacgga
     5881 ttcaaacgcg ccacccgata cagccccgag ctggacagat cgggcaatgc cgccgaagcc
     5941 ttcaacggca ctgcagatat cgttaaaaac atcatcggcg cggcaggaga aattgtcggc
     6001 gcaggcgatg ccgtgcaggg cataagcgaa ggctcaaaca ttgctgtcat gcacggcttg
     6061 ggtctgcttt ccaccgaaaa caagatggcg cgcatcaacg atttggcaga tatggcgcaa
     6121 ctcaaagact atgccgcagc agccatccgc gattgggcag tccaaaaccc caatgccgca
     6181 caaggcatag aagccgtcag caatatcttt atggcagcca tccccatcaa agggattgga
     6241 gctgttcggg gaaaatacgg cttgggcggc atcacggcac atcctatcaa gcggtcgcag
     6301 atgggcgcga tcgcattgcc gaaagggaaa tccgccgtca gcgacaattt tgccgatgcg
     6361 gcatacgcca aatacccgtc cccttaccat tcccgaaata tccgttcaaa cttggagcag
     6421 cgttacggca aagaaaacat cacctcctca accgtgccgc cgtcaaacgg caaaaatgtc
     6481 aaactggcag accaacgcca cccgaagaca ggcgtaccgt ttgacggtaa agggtttccg
     6541 aattttgaga agcacgtgaa atatgatacg aagctcgata ttcaagaatt atcggggggc
     6601 ggtataccta aggctaagcc tgtgtttgat gcgaaaccga gatgggaggt tgataggaag
     6661 cttaataaat tgacaactcg tgagcaggtg gagaaaaatg ttcaggaaat aaggaacggt
     6721 aatataaaca gtaactttag ccaacatgct caactagaga gggaaattaa taaactaaaa
     6781 tctgccgatg aaattaattt tgcagatgga atgggaaaat ttaccgatag catgaatgac
     6841 aaggctttta gtaggcttgt gaaatcagtt aaagagaatg gcttcacaaa tccagttgtg
     6901 gagtacgttg aaataaatgg aaaagcatat atcgtaagag gaaataatag ggtttttgct
     6961 gcagaatacc ttggcaggat acatgaatta aaatttaaaa aagttgactt tcctgttcct
     7021 aatactagtt ggaaaaatcc tactgatgtc ttgaatgaat caggtaatgt taagagacct
     7081 cgttatagga gtaaataaaa atggcaattt ggaattatcg gtactacctt atcccatcag
     7141 ctgctatcag aaataaattt aatgcaaata aagatatcat acttgatgag tatcgatcta
     7201 atggttttca gaattttaat gagaataaaa gttttgaaaa ttactttatc gataatgatg
     7261 ttatattatt atcaataata aatgaagcaa aaaaacagct taaattgaaa gaatcttggg
     7321 ataaagacgc aatcatgttt tgtgataatt ttggtaatag tcttaccgtt tggccagatg
     7381 atatagagtg cgaacttgat ttaagatttg attatactaa atttattcag aaaaccattg
     7441 attgggcaat aaaatataat tgtctacttg taatagaaaa aacaggaaat gtagtttccc
     7501 ctaatataaa taatctgatg tatgaaataa aagcatattt ggaaagcaag ccgtggccca
     7561 tatgaaacct aaactcaaca agtaggatgt gtgcggaacg cacgtatgcg gttctcaagg
     7621 tttgagctaa gaggccgtct gaaaacagaa aaactgtttc agacgacctt tcttttaacc
     7681 agttgccaca gcaaccggac aaaagcagcc tacctccaca tccatatagg caatacaggg
     7741 gagatatttt gtaaattcta cgaatatttt acctgctaaa cagggtagga tatggtatga
     7801 agcgaacatt ggcttaataa acactatgtc aagatcgaat caggctggta ctagattgtt
     7861 gtattccaat tatggattgc tatatataac aactgatcat tatatctctg caacacggtt
     7921 tgtagcttgg aaataggagt ataacttatg caattagaga ttatcggtag taaaatttat
     7981 acggaacaag attttcataa tcaaatttca aaaatatttt ctatacaaga ttattatggg
     8041 aacaatcttg atgctttatg ggatttatta agcacaaatg tagaacgacc gattactttg
     8101 gtatggaaag atgctatgtt ctcaaaaaat caattagaaa atatatttat tgaaatcgta
     8161 aatgttctag aaagagttaa gaaacaagat gaggattatg gattcgaaga aaaatttaat
     8221 tatattttag agtaagtaaa ccctaattac attacgtaca caggcttaaa actccccaga
     8281 gccaattaag caagccgtaa cccatataaa acttaaactc aacaagtagc atgtgtgcgg
     8341 aacgtacgca tgcggttctt aaagtttgag ctaagaggcc gtctaaaaac agaaaaaccg
     8401 tttcagacgg tctttgttta acgccaccga tccagcgggt tacaaagcgc agtcaatgcc
     8461 gctgcgcctt atgcctccga agcaataggc agaacatttg gacacggtga aaacaaaaac
     8521 gaaaccgccc aagccgtcgg acatttcctt ttaggagcag ctattgcccg cgtcaacggt
     8581 ggtaattttg ctgccggcgg ctcggcagca gttgcagctg aaaaggcggc ggaacatctt
     8641 gcccaacagt ataacgacgg taaaaccgca atcgatccgc aaacaggcga gttcaatgcc
     8701 aacctgctgc cggaacatat caaagaggaa atcaaatcaa agagcggggt gattgcatcg
     8761 ctgacgggcg cggccgtggg cggcacgccg gtagatgcgc aaaccggagg tgcggtcgga
     8821 cagaatgcgg tggaaaacaa cctctatctg acatcggaag ccttaaagaa ggacaagcag
     8881 acagctcgta aaatttattc cgtcataaaa gagcaagtca agcatgaatg cagttccaca
     8941 ggaagaatta ccgaatgtcg tcaaaatata ggacgcatta tcgaatttac ccaagacaaa
     9001 cgctttgaca gtaggtttaa ggacttaaaa aaagaatcct tatattacct aaataaacat
     9061 cctgatttag tagcctctta tttgaaggct gaatacgaaa agctggatag ggaagacaaa
     9121 agtatcctgc accgctacat ctcacccggg gctgaaatcg tttcgggcag tttgggggtt
     9181 gttctttcag gagtagccgg aggcggatct tgtgccgaga ctttcggctt aggctgtgcc
     9241 gccgctttgg ttggtgtaac gtcttcctac gatcatgtca ttactgggac gaaaaacttc
     9301 ggaaaaaaag ccagcgagca acgaccgacg attgcggttc aggccttgaa gcagttgggg
     9361 ctgtcggagc aggctgcgga atatgttcag ttctctatag atttgttcag tgtgggtaaa
     9421 tcggggggcg gtatacctaa ggctaagcct gtgtttgatg cgaaaccgag atgggaggtt
     9481 gataggaagc ttaataaatt gacaactcgt gagcaggtgg agaaaaatgt tcaggaaacg
     9541 agaagaagga gtcagagtag tcagtttaaa gcccatgcgc aacgagaatg ggaaaataaa
     9601 acagggttag attttaatca ttttataggt ggtgatatca ataagaaagg cacagtaaca
     9661 ggagggcata gtctaacccg tggtgatgta cgggtgatac aacaaacctc ggcacctgat
     9721 aaacatgggg tttatcaagc gacagtggaa attaaaaagc ctgatggaag ttgggaggtg
     9781 aaaacgaaaa aaggtgggaa agtgatgacc aagcacacca tgttcccaaa agattgggat
     9841 gaggctagaa ttagggctga agttacttcg gcttgggaaa gtagaataat gcttaaggat
     9901 aataaatggc agggtacaag taaatcgggt attaaaatag aaggatttac cgaacctaat
     9961 agaacagcat atcccattta tgaatagtaa tatttatgaa aaattaggag attaatgatg
    10021 aaaagaatta agtgcttttg tgataaattt ccatcaggag atacatttag aatgtgtatc
    10081 attctggatg actatgataa tagggttgat tattatgtag gaatatatga ttacattacg
    10141 tctaccttaa tgagcgatat ttactatcga tccacgattg atgagcattt caagattata
    10201 gaattaatag aaaataatcc aaatgaaatt tatgatgatg gcggtggtca acaattttgc
    10261 ctagaatttc atcatgataa ggtcattttt taccacaatg aatttgatga agaagatggt
    10321 tatccagtat taagctgttc gctgcatact tttaaaactg ctttaattgc ttggaatgct
    10381 tttttgcaat tgcctaaaag tattcattcg gtggtggaga ctgtgattga ggaataagca
    10441 taattagctt aatgaataga atcagcgata tagattggac tgcaaatcca cgcttatacg
    10501 ctgtgccatg attaagatgt tagaacttgt attgaataca agttctcata aacgaatggc
    10561 agtaagcatt tgatttagat aaaatccttg aattagaata atcaggtcta agagctcgac
    10621 aggacaaatg aggctggcaa ccaaggattt ggcggaagcc attaggaaag gacaggttcg
    10681 caaatcaagc tttaacacag aacaattaag ggcaattgaa aaaggagaat ctaaaatacc
    10741 ggattacact tggcatcatc atcaagatac aggaaggatg caattgattc gtgaaggctt
    10801 gcatcatgat accggccata ttggttggga agcaatgaac aaaggaaggt aactatgtgg
    10861 aaaatcataa aagaggatag tgatgattta g
//
LOCUS       DAWGRZ010000054        10737 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000054 DAWGRZ010000000
VERSION     DAWGRZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10737
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.054"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(13..216)
                     /locus_tag="WHT44_000994"
     CDS             complement(13..216)
                     /locus_tag="WHT44_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533191.1"
                     /translation="MDTTLKYKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKAKYDVQRQKLKEAGWSDDAV"
     gene            complement(194..565)
                     /locus_tag="WHT44_000995"
     CDS             complement(194..565)
                     /locus_tag="WHT44_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ2533192.1"
                     /translation="MAAYGVSDGIFTFTEQAVRIHRRFFGNFGYGGGRCYNNGLFLQG
                     NIMEATVYLEDNEYIALCDLLKLVGLAESGGQAKAFIAEGLVLRNGETETRKTAKIRG
                     GEVIEFDGARLEIADGYDPEV"
     gene            965..4399
                     /gene="dnaE"
                     /locus_tag="WHT44_000996"
     CDS             965..4399
                     /gene="dnaE"
                     /locus_tag="WHT44_000996"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ2533193.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPIGAADVRIGNPDAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNVHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARTAALKY
                     AAWFPDAFYMELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPGQFFIPPETMAERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDYLIKLSNEGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQSNRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLEKAMETEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIIDVGKIPLDDQVAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESITAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLDR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            4547..4696
                     /locus_tag="WHT44_000997"
     CDS             4547..4696
                     /locus_tag="WHT44_000997"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533194.1"
                     /translation="MHDTDSTKGKNGIFQSIGIKTALSLQKYQIDKPGKEIRPCRLKK
                     RPPVR"
     gene            complement(4833..5765)
                     /locus_tag="WHT44_000998"
     CDS             complement(4833..5765)
                     /locus_tag="WHT44_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ2533195.1"
                     /translation="MLSQLEYGIAKENRPNRQADRFAGSVLPLAASLTHKPNIMKPIP
                     TDTFQPAILPQAFETEIKSTCTGRIYRIQTATLGEIPSEGYPVLFVLDGEAFFPALFN
                     IMQSLMNNPVTRSNAPCLIVGIGYTTGSVRDLAQRAADYTPPLGDNATADERQQFGQA
                     DRFAAFIDSELTAFLESRYTLNRNETAVFGHSFGALFGLYSLLSHRRFRRHWLVSPSI
                     WWHNRRILDFMPSENRLNGIDVCLNIGALERGSDCKRREERDMAGQAEQMAAELDRHG
                     AAVFFREYPNADHGNVPFYSLTDCVEYLRKAWQR"
     gene            complement(5835..7949)
                     /locus_tag="WHT44_000999"
     CDS             complement(5835..7949)
                     /locus_tag="WHT44_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ2533196.1"
                     /translation="MSVFRINMTAATVLAALSSSVFAAQTEGLETVHIKGQRSYNAIA
                     TEKNGDYSSFAATVGTKIPASLREIPQSVSIITNQQVKDRNVDTFDQLARKTPGLRVL
                     SNDDGRSSVYARGYEYSEYNIDGLPAQMQSINGTLPNLFAFDRVEVMRGPSGLFDSSG
                     EMGGIVNLVRKRPTKAFQGHAAAGFGTHKQYKAEADVSGSLNSDGSVRGRVMAQTVGA
                     SPRPAEKNNRRETFYAAADWDINPDTVLGAGYLYQQRRLAPYNGLPADANNKLPSLPQ
                     HVFVGADWNKFKMHSHDVFADLKHYFGNGGYGKVGMRYSDRKADSNYTFAGSKLNNTG
                     QADVAGLGTDIKQKAFAVDASYSRPFALGNTANEFVIGADYNRLRSTNEQGRSTLSKS
                     VALDGFRALPYNGILQNARAGNKGFNHSVTEENLDETGLYAKTVFRPLEGLSLIAGGR
                     VGHHKIESGDGKTLHKASKTKFTSYAGAVYDIDGSNSLYASASQLYTPQTSIGTDGKL
                     LKPREGNQFEIGYKGSYMDDRLNTRVSFYRMKDKNAAAPLDSNNKKTRYAALGKRVME
                     GVETEISGAMTPKWQIHAGYSYLHSQIKTASNSRDEGIFLLMPKHSANLWTTYQVTSG
                     LTIGGGVNAMSGITSSAGIHAGGYATFDAMAAYRFTPKLKLQINADNIFNRHYYARVG
                     SESTFNIPGSERSLTANLRYSF"
     gene            8109..8768
                     /locus_tag="WHT44_001000"
     CDS             8109..8768
                     /locus_tag="WHT44_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ2533197.1"
                     /translation="MTTPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRTLLEMPSETAVADITRTYSAHYLNPNNRNMSLFPDALPCLDKLKAQ
                     GYWLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGICGELGLDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLSAPHLAVLDGLSELPGFLAQHY
                     A"
     gene            complement(8856..9824)
                     /gene="ispH"
                     /locus_tag="WHT44_001001"
                     /gene_synonym="lytB"
     CDS             complement(8856..9824)
                     /gene="ispH"
                     /locus_tag="WHT44_001001"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ2533198.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVRQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(9853..10350)
                     /locus_tag="WHT44_001002"
     CDS             complement(9853..10350)
                     /locus_tag="WHT44_001002"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ2533199.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKY"
     gene            complement(10556..10660)
                     /locus_tag="WHT44_001003"
                     /pseudo
     CDS             complement(10556..10660)
                     /locus_tag="WHT44_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         2445 a         3051 c         2825 g         2416 t
ORIGIN      
        1 agccgaaccg tttcagacgg catcgtccga ccaacccgct tctttcaatt tctgccgttg
       61 cacgtcgtat ttggctttat ccgcccagta aatcgtctga atgcacgcct cgccgcagtc
      121 gctgccgcag cattcccacg actcgggtcg gacgggttcg tctaaaagcg gctcgcccaa
      181 aagggcttcg gctttatact tcagggtcgt atccatcggc gatttccaag cgagcgccgt
      241 caaactcgat gacttcgccg ccgcgtattt tggcggtttt acgggtttcg gtttcgccgt
      301 tgcgcaacac cagcccttcg gcgataaacg ctttcgcctg tccgccgctt tcggcaagtc
      361 cgaccaattt caagaggtcg cacaaggcga tgtattcgtt gtcttcgaga tagacagtgg
      421 cttccataat gttcccttgc agaaagaggc cgttattgta gcacctgccg ccgccgtacc
      481 caaaattacc gaaaaaccgg cgatgtatcc gcaccgcctg ttccgtaaaa gtaaaaatgc
      541 cgtctgaaac cccatatgcc gccatccgtt caaagaaatc ctgcccaacg gcagactgca
      601 aatcctgttc cccgacgaat ccgcattgac gctgatgcac atcctcaaac gcgaactgcc
      661 cgatacaccg gcaatcggca tcaaaacgaa atcaaaaacc tgtttgaacg taaaattaat
      721 ttaactgctt gccatccgtc cgaataaggc atatagttct ttataactag tttgatagtc
      781 ctttatatct atcaatactc cttgggaagc ctccgccata cggcaggagg catttttttg
      841 ccgtagtaaa agctcaaaaa catttgcagg tcatgccgtc tgaacccgaa acggcattac
      901 ctacaccgcc atctaaagac aaccctgcta caatacgcct tttattgtcc acgccgattt
      961 tgccatgacc gagccgacct acattcccct gcgcctgcat accgaatttt cgattaccga
     1021 cggtatggtg cggattaaaa aactgattgc caaagcgcag gaatacggtt tgcctgcttt
     1081 gggcatcagc gatttgatga acgaattcgg tttggtgaaa ttttataaag cctgccgcag
     1141 cgcggggatt aagcctatcg gcgcggcgga tgtgcggata ggcaatccgg atgcgcccga
     1201 caagccgttc cgcgctatgc tgattatccg taacgatgcg ggctatctgc gcttgagcga
     1261 gcttctgacg gcggcttatg tcggcaaaga ccgcaatgtc catcatgcgg aactcaatcc
     1321 cgaatggctg gaaaacggcg acaacagcgg cttgatttgt ttgagcggcg cacattacgg
     1381 cgaagtgggc gtgaatctgt tgaacggcaa tgaagacgcg gcgcgtacgg cggcgttgaa
     1441 gtatgcggcg tggttccccg atgcgttcta tatggagctg caacgcctac ccgaacgccc
     1501 cgaatgggag gcttgcgttt cgggcagcgt gaagctggcg gaggaattgg gtttgccggt
     1561 ggtggcgacg catccgacac agtttatgag ccgcgacgat ttcaacgcgc acgaggcgcg
     1621 agtgtgtatc gcaggcggct gggtattgac ggacaagaaa cgtccgcgcg atttcacgcc
     1681 gggccagttt ttcattccgc cggaaaccat ggccgaacgt ttcgccgatt tgcctgaagc
     1741 cttggaaaac acggtagaaa ttgccaaacg ctgcaacctg cacatcacat tgggcaaaaa
     1801 cttcctgccc cttttcccca cgcccgacgg tttatcactc gatgactatc tcatcaaact
     1861 ctccaacgag ggtttgcagg aacgtatggt tcagctttat cccgacgagg cggagcgggc
     1921 ggcaaaaatg ccggaatatc aggaacgttt ggattttgag ctgaacatca tcatccaaat
     1981 gaaattcccc ggctatttcc ttatcgtaca agactttatc aactgggcga aaacacacgg
     2041 ctgtccggtc gggccgggcc gtggttcggg cgcgggttcg ctggtggcgt attcattgaa
     2101 gattaccgac cttgatccgc tcaaatacgc gctgctgttc gagcgtttcc taaaccccga
     2161 acgcgtttct atgcccgact tcgacgtgga cttttgccaa agcaaccgcg gccgcgtgat
     2221 tgaatatgtg cgcgagaaat acggcgcgga ggcggtcagc cagattgtta ccttcggcac
     2281 gatgtcgtcc aaagcggtca tccgcgacgt cgggcgcgtg ttagagctgc cgtttatgct
     2341 gtgcgacaaa ctgtccaagc tgattccgtt ggaagccaac aaacccctga gtttggaaaa
     2401 agccatggag accgagccac agattcagga attaatcgaa gcggaagaag cggacgaact
     2461 gattacgctg gcgaaaaagc tggaagattt aacgcgcggt ttgggtatgc acgcaggcgg
     2521 cgtgttgatt gcgccgggca agatttccga ttacagcccc gtgtatcagg cggacgaatc
     2581 cgcctcgccc gtatccatgt acgacaaggg cgacgtggaa gatgtgggtt tggtgaaatt
     2641 cgactttttg ggtctgcgca acctgaccat tatcgaaatg gcgcagaaca acatcaaaaa
     2701 cactaccggc gacatcatcg atgtcggcaa aatcccgctt gacgaccagg tcgcctacca
     2761 aatcttccgc gatgcgaaca ccaccgccgt cttccagttt gagtcgaccg gcatgaaaaa
     2821 aatgctgaaa acggcgcaca cgaccaagtt tgaagaactc atcgccttcg tatcgctcta
     2881 ccgccccggc ccgatggaca acattcccga cttcgtcgca cgtatgaagg ggcaagaatt
     2941 ccaatacatc catccgctac tcgaaggcat cctcgcgccg acctacggga ttatggtgta
     3001 tcaggaacaa gtgatgcagg cggcgcaaat tatcggcggc tactcgctcg gcggcgcgga
     3061 cctgctgcgt cgcgccatgg gtaagaaaaa acccgaagaa atggtgaaac accgcgaaat
     3121 cttcgccgaa ggcgcggcaa aacaaggcat ttcgcgcgaa aaatccgacg aaatcttcaa
     3181 ctacatggaa aaattcgccg gctacggttt caacaaatcc cacgccgccg cctacgccct
     3241 gatttcctac cagaccgcat ggcttaaagc gcactacccc gccgaattta tggcggcgac
     3301 catgtcgtcc gaattggaca acaccgacca gctcaagcat ttctacgacg actgccgcgc
     3361 caacggcatt gagttcctgc cgcccgacat caacgaatcc gactaccgct tcacgccgta
     3421 tccggacatg aaaatccgct acgcgctcgg cgcgattaaa ggcacgggcg aagccgccgt
     3481 cgaatccatc accgccgcgc ggcaaagcgg cggcaagttt accggtctgt tggacttctg
     3541 cgagcgcgtc ggcaaagaac acatgaaccg ccgcaccctc gaggccctga tacgcggcgg
     3601 cgcgttcgac agcatcgaac ccaaccgcgc catgctcttg gcgaacatcg acctcgctat
     3661 ggacaacgcc gaccaaaaag ccgccaacgc caatcagggc gggcttttcg acatgatgga
     3721 agacgccatc gaaccggtgc ggctcatcga cgcgccgatg tggagcgaat cggaaaaact
     3781 cgccgaagaa aaaaccgtca tcggctttta cctgtccggc cacccgttcg gcccgtatgc
     3841 ccaagaagtc cgccaaatcg caccgaccaa attagaccgt ctgaagccgc aagacagcgt
     3901 gcgcctcgcc ggattcgtta ccgccgtgcg tacgatgatg ggcaaacgcg gcaaaatcgc
     3961 cttcgtcagc ctcgaagatt tgagcggaca ggttgaaatc atggtcggcg gtcagacgtt
     4021 ggaaaactgc gccgactgcc tcaaagccga ccaagtgctg attatcgaat ccaaagtcag
     4081 ccgcgacgac tacggcggcg gcgacgggct gcgtattctg gcaaaccaag tcatgaccct
     4141 gcaaacggcg cgcgaacgct acgcccgcag cctcagcctc gccctcgccc cgcatcacga
     4201 catcggcgga ctggtacggc tgctcgccgc ccaccaactg cccgacacgc cgcgcatccc
     4261 gctgcaactg tcgtatgcca acgaaaaagc gtcgggcagg cttcaagtgc cgccgaaatg
     4321 gacggttaca ccgagctccg cattgttcgg cgaactggaa acattgctcg gcagccggtc
     4381 ggtgcgcgtc aactggtaac ccaaaatata aatgccgtct gaagcccaaa aaccggtttc
     4441 attcgtactt tattcgaatg attgaataaa agtaactgcc aagaaaaacg tattttttgg
     4501 ttatttcgcc agtctaaata gagcaaccgg gacgattgat atccgtgtgc atgacacaga
     4561 cagcaccaaa gggaaaaacg gcattttcca aagtatcggt atcaaaaccg ccctttcact
     4621 ccaaaaatac caaatcgaca aaccgggcaa agaaatcaga ccgtgccgcc tgaaaaaacg
     4681 cccacccgtc cgttaaatta acctatccct gtttcagacg gcctgaagca gggattttta
     4741 tatcaaaata aaatgagaaa gggagcaata acccttaggt agctcttgtt attttccgat
     4801 gcaaaacaaa gcagtcatat atttaattcc ccctacctct gccaagcctt cctcaaatat
     4861 tcgacgcaat cggtcagcga gtagaacggg acattgccgt ggtcggcatt cggatattcc
     4921 cggaaaaaga cggcggcccc gtgcctgtct aactctgccg ccatttgttc ggcctgccct
     4981 gccatatcgc gttcttccct gcgtttacaa tcgctacccc gttctagcgc gccgatgttg
     5041 aggcagacat cgatgccgtt tagccggttt tcagacggca taaagtcgag tatccgcctg
     5101 ttgtgccacc aaatcgatgg ggatacgagc caatgccgtc tgaaacggcg gtgggaaagc
     5161 agggaataca gtccgaacag tgcgccgaac gagtgtccga atacggcggt ttcattgcgg
     5221 ttgagggtgt agcggctttc taaaaaggcg gtcagctcgc tgtcgataaa ggcggcgaag
     5281 cggtctgcct gtccgaactg ctgccgttcg tctgctgtgg cgttgtctcc aagcggcggc
     5341 gtgtagtcgg cggcacgttg tgccaaatcg cgcacactgc ctgtcgtgta gccgataccg
     5401 acaatcaggc agggggcgtt gcttcgggta acggggttgt tcatcagcga ctgcatgatg
     5461 ttgaaaagtg cggggaaaaa ggcttcgccg tcgaggacaa agaggacggg atagccttca
     5521 gacggtattt cgccgagtgt tgccgtctga atccgataga ttcgccccgt gcaggtggat
     5581 ttgatttcgg tttcaaaggc ttggggcagt atggcaggtt ggaatgtgtc ggtcggtatg
     5641 ggtttcatga tgttcggctt gtgggtcaga ctggcggcaa gcggtaaaac cgaaccggca
     5701 aagcggtctg cctgccggtt cggtctattt tccttcgcaa tgccatactc cagttgtgag
     5761 agcatagggt atgccgcgca gcttgttgta gtttgatgat gctgcggctg ccgtttcaga
     5821 cggcatattg gtctttaaaa actgtaacgc aggtttgccg tcaggctgcg ctccgaaccg
     5881 ggaatgttaa aggtgctctc gctgccgacg cgggcgtagt aatggcggtt gaagatgttg
     5941 tcggcgttga tttgcagctt cagtttgggc gtgaagcggt atgccgccat cgcatcgaac
     6001 gtggcataac cgcctgcatg tatccctgca gatgaagtaa tgccgctcat cgcgttcacg
     6061 ccgccgccga tggtcagccc ggacgtaact tggtaagtcg tccacaggtt tgcgctgtgt
     6121 ttgggcatca gcaggaagat gccttcgtcg cgcgaattgg aggcggtttt gatttggctg
     6181 tgcaggtagc tgtaacctgc atggatttgc catttcggtg tcatcgcgcc gctgatttcg
     6241 gtctcaacac cttccatcac gcgtttgccc aatgcggcgt aacgggtttt tttgttgttt
     6301 gagtccagcg gtgcggcggc gtttttatcc ttcatgcggt agaacgaaac ccgggtattg
     6361 aggcggtcgt ccatgtagct gcctttgtag ccgatttcaa actggttgcc ttcgcgcggt
     6421 ttgagcagct tgccgtcggt gccgatgctg gtttgcggtg tgtagagttg ggaggcggaa
     6481 gcgtacaggc tgttgctgcc gtctatatcg taaaccgcgc cggcgtagct tgtaaatttg
     6541 gttttcgaag ctttatgcag ggttttgccg tcgcccgact cgattttgtg atgtcctaca
     6601 cgtccgcctg caatcaacga caaaccttcc agaggacgga acaccgtctt ggcatacaaa
     6661 ccggtttcgt cgaggttttc ttcggtaacg gagtgattga aacctttgtt tccggcgcgg
     6721 gcgttctgaa gtatgccgtt ataaggcaaa gcgcggaaac catctaaagc gacgcttttt
     6781 gacaaagtcg aacgcccttg ttcattagta ctgcgcaagc ggttgtagtc tgcaccaatc
     6841 acaaattcgt tggcggtgtt gcccaaggca aacggacggc tgtaacttgc gtcaaccgca
     6901 aaggcttttt gtttaatgtc cgtacccaaa cccgctacgt cggcttgtcc ggtattgttg
     6961 agtttgctgc ccgcaaacgt ataattggaa tcggctttcc gatcggaata gcgcataccg
     7021 actttgccgt agccgccgtt gccgaagtaa tgtttcaaat cggcgaacac gtcgtggctg
     7081 tgcattttaa atttgttcca atccgcgccg acaaatacgt gttgcggcag ggacggtaat
     7141 ttgttattgg catcggcagg caggccgttg tacggcgcga ggcggcgttg ctggtaaaga
     7201 tagcccgcgc ccaaaaccgt atcggggttg atgtcccaat ccgccgccgc gtagaaggtt
     7261 tcgcgccggt tgtttttctc ggcgggacgc ggagacgcgc cgacggtctg cgccatcacg
     7321 cggccgcgca cgctgccgtc tgaattgagg ctgcccgata cgtccgcctc ggctttatat
     7381 tgtttgtgcg taccgaaccc tgccgccgca tgaccttgga acgctttggt cgggcgtttg
     7441 cgcaccagat tcacgatgcc gcccatctcg ccgctgctgt cgaacagtcc gctcggcccg
     7501 cgcatcactt ccacgcggtc gaaggcgaac aggttgggca gcgtgccgtt gatactctgc
     7561 atctgcgcgg gcaggccgtc gatgttgtat tcgctgtatt cgtaaccgcg cgcgtaaacc
     7621 gaagagcgtc cgtcgtcgtt gctcaacacg cgcaggccgg gcgttttgcg tgccaactgg
     7681 tcaaacgtat caacattgcg gtctttgacc tgctggttgg taatgatgct gacggattgc
     7741 ggaatttcgc gcaaagaagc ggggattttt gtaccgacgg tggcggcaaa cgagctgtaa
     7801 tcgccgtttt tctcggtggc aatcgcgttg taagaacgct gacccttaat atggacggtt
     7861 tccaaacctt ccgtttgtgc ggcaaaaacc gaagacgaga gtgctgccaa aaccgtggcg
     7921 gcggtcatat tgatgcggaa aactgacata aactgtccca ttcacataaa tgataatggt
     7981 tctattttaa taaagcgcaa cgcggcttgt tcggaaaaac atatcgcgca gccgacaaat
     8041 tttgtcgaaa atgcgacacg tctgcgtttt ccgcataaaa tttgcttttt tactgcaacc
     8101 aacctgctat gaccacgccc aaactcatca tcttcgactg ggacggcacg cttgccgata
     8161 cgacccaacc catcatcgac accatgcgcc gcagcttcgc cgaatgcggt tttccgccgc
     8221 ccgaagcgga acgcgtccgc agcctgattg gctacagcct gcccgaaatc atccgcaccc
     8281 tgctcgaaat gccgtctgaa accgccgttg ccgacatcac acgcacttat tccgcacatt
     8341 acctcaatcc caacaaccgc aatatgtcct tatttcccga tgccctgccc tgtctggaca
     8401 agctcaaagc acaaggatac tggcttgccg tcgccacggg caaagggcgg gcgggtttgg
     8461 acaacgccat cagtcaaacc gccaccggcg gctattggct cgccaccgcc tgcgcggggg
     8521 aatatccctc caaaccctcg cccgaaatgg tattcggaat ctgcggcgaa ctgggactcg
     8581 acccgaaaga ggcattggtc gtcggcgata cggcgcacga cctgcatatg gcggcaaacg
     8641 caggcgcggc ggcagtcggc gtggccaccg gcgcacattc gcgcgaacag ctccttagcg
     8701 caccgcatct cgccgtattg gacggtttgt ccgaactgcc cggttttctt gcacaacatt
     8761 acgcctgatt ggtttccgca tccggcacac ggcaaaaatg ccgtctgaag cctgttcaga
     8821 cggcatttgt gttgcccaaa cattcaacgc ctgcgtcaac gtttgcacaa atcgggtttg
     8881 gtttcgccct cgcggcgcaa ctctttgggc aggacgaaca caatgctttc ttccgcaccc
     8941 tcgccttcgc gtacggtttc gtgcccccat ccgcgtatgg ttgccagtac ttcccgcacc
     9001 aacacttcgg gcgcggacgc gcctgccgtt acgccgactt tgtttttgcc ctcaaaccat
     9061 gcgcgttgca ggtagcctgc attatccacc atatacgcat cgattccgcg cgatgccgcc
     9121 acttcgcgca agcggttgct gttggacgaa ttgggcgaac cgaccacaat cacgatgtcg
     9181 cactgttctg ccaactcttt gacggcggtt tgccggttgg tcgtcgcata gcagatatct
     9241 tccttgtgcg gattgcggat attggggaaa cgcgcgttca gcgcggcgat gatgtctttg
     9301 gtttcatcga ccgagagcgt ggtttggctg acataggcga gtttgtcggg gtttctgact
     9361 tcgagttttg ccacatctcc gaccgtttcg accaaaagca ttttgcccgg cgcaagctgc
     9421 cccatcgttc cttcgacctc gacgtgcccc ttatgcccga tcatgatgat ttcacagtct
     9481 tgggcatcca gtcgggcgac ttccttatgc actttcgtca ccagcgggca agtcgcatca
     9541 aacacgcgga aaccgcgctc cgccgcttct tgccgcaccg ccttcgatac gccgtgtgcc
     9601 gaataaacca gtgtcgcgcc cggcggcact tccgccaagt cttcaataaa caccgcacct
     9661 ttttcacgca ggttgtccac gacgaatttg ttgtgaacga cttcgtggcg cacataaatc
     9721 ggcgcgccga actcttccaa agcacgttcg acaatactga ttgcccgatc cacaccagcg
     9781 cagaagccgc gcggattggc aaggatgatg gttttctcgt tcataagccc ggtatttcgt
     9841 tttcagacgg catcaatatt tttcttcttg ggttttacgg tggacgatgt tgtccaacac
     9901 cgccaacacc gcaccgacgc agataaagct gtcggcaata ttaaaggcgg gataaaacca
     9961 attttgccaa taaaacaata agaaatcgac gacatgaccg tgtatcaggc ggtcgatgac
    10021 attgcctaac gcaccgccga taatcattgc cgcacccgtt ttgccgaggg ttgcaaactc
    10081 atcgcgcaag atggcgcgta ccaaatacgc gctcaccgcc accgccagca ccaaaaaaaa
    10141 gtatttttgc cagccgccct gatcggcaag gaagctgaac gccgcacccg ggttgtacac
    10201 cagcgtcaga tcgaaaaagg aaggaatgac attgacgcgt tcccgatact gaaacgacga
    10261 cagcaccgcc cacttcgacc actggtccag cacgatggcg gcaagtgcca atacccaata
    10321 gcgcgtttta cttgaaacag atgaagacat atttttcaac agccggtaaa agagtaccat
    10381 tttacccgaa aacccccttt cctgtacccg aaacggcaaa tgccgtaatc ttaaaacccg
    10441 tcattcccga caacaccgta atctcgaaac ccgtcattcc cgcgtaggcg ggaatccaga
    10501 cctgtccgca cagaaactta tcggataaaa acagttgccc aaaccccgcg ttctatagtg
    10561 gattaaattc aaaccagtac ggcattgcct cgccttgccg tactatttgt actgtctgcg
    10621 gcttcgttgc cttgtcctga tttaaattta atccactata gattcccact tccgtgggaa
    10681 tgacggttca gttgcattcc gacaacaccg taatcttgaa atccgtcatt cccgcgc
//
LOCUS       DAWGRZ010000055        10483 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000055 DAWGRZ010000000
VERSION     DAWGRZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10483
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.055"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(278..1075)
                     /locus_tag="WHT44_001004"
     CDS             complement(278..1075)
                     /locus_tag="WHT44_001004"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ2533200.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSICCGWWSLPFWVFTV
                     FALQFFRDPAREIPLNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QTESEPESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
     gene            1230..4079
                     /gene="uvrA"
                     /locus_tag="WHT44_001005"
     CDS             1230..4079
                     /gene="uvrA"
                     /locus_tag="WHT44_001005"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ2533201.1"
                     /translation="MCNHHPQHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPTVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHQ
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAQCENSVTGQYLSGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPFD
                     DIHGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIAKGSPEQVAKVKGSYTGKYLKVVLR"
     gene            4113..5708
                     /locus_tag="WHT44_001006"
     CDS             4113..5708
                     /locus_tag="WHT44_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="funZ protein"
                     /protein_id="HEZ2533202.1"
                     /translation="MKPLKTLEFGFVDAANYRRRENKDLFNRIFVKGEYLDELCEPNI
                     SFLIGEKGTGKTAYAVYLTNNFYKNIHATTKFVRETDYSKFIQLKKARHLTVSDFTSI
                     WKVILYLLISNQIKCKENGILSSIFNKFKALDEAINEYYYGAFDPEIVQAITLIENSK
                     EAAEMIFGKFVKLGEEESQQITFTESKFQANLGFIERKFKDALSQLKLKDNHILFIDG
                     IDIRPSQIPFDEYHECVKGLANAIWMLNNDIFPSIKDSKGRMRVVLLIRPDIFDSLGL
                     QNQNTKLQDNSVFLDWRTDYKSYRSSKIFGVFDHLLRTQQEKQDSLEKGNSWDYYFPW
                     NAPNLHDEYKNLTSFISFLRKSYYRPRDILQMLTLLQKNKKSKEDYVVAEDFDNTSFQ
                     REYSIYLLGEIKDHLLFYYSQSDYQNFLKFFEFLNGKDRFKYSDFLKAFERLKKHLQT
                     TSVEIPKFMSTANEFLQFLFDLNVIAYLDNPEDETKPYIHWCFKDRNYANISPKIKTE
                     TEYLIFSGLSKALDVGTPFKNKQ"
     gene            complement(6015..6239)
                     /locus_tag="WHT44_001007"
     CDS             complement(6015..6239)
                     /locus_tag="WHT44_001007"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533203.1"
                     /translation="MPSETGNRVSDGILFVISKRGSLNIPTLFLPFWGSQRPLQNSPK
                     SPKFPKFPPRHLGDFGEFCKGLGLSVPVCA"
     gene            complement(6268..7158)
                     /gene="sucD"
                     /locus_tag="WHT44_001008"
     CDS             complement(6268..7158)
                     /gene="sucD"
                     /locus_tag="WHT44_001008"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ2533204.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVSKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(7169..8335)
                     /gene="sucC"
                     /locus_tag="WHT44_001009"
     CDS             complement(7169..8335)
                     /gene="sucC"
                     /locus_tag="WHT44_001009"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ2533205.1"
                     /translation="MNLHEYQAKELLASYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRITFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVQGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVNVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            complement(8434..8721)
                     /locus_tag="WHT44_001010"
     CDS             complement(8434..8721)
                     /locus_tag="WHT44_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533206.1"
                     /translation="MNKEIVGIFFIPAGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            complement(8846..10279)
                     /gene="lpdA"
                     /locus_tag="WHT44_001011"
     CDS             complement(8846..10279)
                     /gene="lpdA"
                     /locus_tag="WHT44_001011"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ2533207.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
BASE COUNT         2719 a         2660 c         2440 g         2664 t
ORIGIN      
        1 ctgtaacgtt aagttcccgg attcccactt tcgtgggaat gacgcggaaa ggtttctgtt
       61 ttttaagtgt taggtaacta ataagtcgtc attcccgcgc aggcgggaat ccagtccgtc
      121 cggtttcagt cgtttccgat aaatcgtcgt aacgttaagt ttctagattc ccactttcgt
      181 gggaatgacg gtgtgaacat tagcaaaaat tcaaaaaacc aaacttcctc attcgtgtga
      241 atgatggtac tgatattttc tatttcagtc cgcaaaatta atctttcaac acatcttgca
      301 cagccgcctg aatcttagcc gccgctgcct cgatttgccg ttgttcggca gatgggtttg
      361 ccgctgtttc aaccggagca gtttgtaaag caggctcaga ttcaggctca gattcagttt
      421 gcggcgcagt cagcggcaaa cgcgccaata cagtgctgac gccggttact ttatcgccaa
      481 tcgccacttg cgcctgcgca tcgacaggca gatacatatc cacgcgcgaa ccgaagcgga
      541 taaagccata acgttcgccg cgggacagtt tcgcacctgc ttgggtgtag cacaaaatac
      601 ggcgcgccac caaaccggcc acttgaacaa aagtaatttc acgacctgaa gccgtagtcg
      661 ccaacaccgc attacgttcg ttttccgtgc tggctttgtc caaatccgca ttcacgaatt
      721 tgcctttgtt atagaccact ttcgttaccg tacaatcggc aggcgatttt tgcgaatgca
      781 cgttgaacac gttcataaaa atactgattt tcaaagcatc gacatcacga tacggatcgc
      841 gtgcgcgttc gaccaccacg atacggccgt caaccgggct caacaccgct tcaggattta
      901 gcggaatctc acgcgcaggg tcgcggaaaa actgcaatgc aaatacggta aacacccaaa
      961 acggcaaaga ccaccagccg caacatatcg acaccagcag gctcaaagcc aaaccgccgc
     1021 caataatcgg ccagccctca cgggcgataa tcgggtgggg gtaaagacgg ttcatttgtc
     1081 atccttttgt tcaagcgggt taaaattgcc gacattatag cggaatctgc cgtactccgc
     1141 tggcaaatgc cgtctgaacg cggcttcaga cggcatccga gaaagtgcta caataccggt
     1201 tttcctttat ttttcaggcg gcatccatta tgtgcaacca tcatccccaa cattcgcacg
     1261 acaacgacac catccgcatc cgcggcgcgc gcacgcataa tttgaaaaac atcgatttag
     1321 acattccgcg ccacaaactc gtggtggtaa caggattgtc gggcagcggc aagtcgtcgc
     1381 tggcgtttga cacgctgtat gccgaagggc aacggcgtta tgtcgagagc ctttccgcct
     1441 atgcgcggca gtttttgcag atgatggaca aacccgatgt cgatttaatt gaaggtctgt
     1501 cgcctgcaat ttccatcgag caaaaatcca ccagccacaa cccacgctcc accgtcggta
     1561 cggtaactga aatccacgac tatctgcgtc ttttatacgc ccgtgtcggt accccgtatt
     1621 gtcccgaaca caagctgccg ctgtcgagcc aaaccgtatc gcagatggtc gatgccgtat
     1681 tgaaactgcc ggaagacacg cgtgtgatga ttcttgcccc caccgtgcgc gagcgtaagg
     1741 gcgagtttgt cgatttcttt gccgacttgc aggcgcaggg ttttgcgcgg gtgcgcgtgg
     1801 acggcgaggt ctatcagttg gacgaagtgc cgaagctgga aaaaaacatc aagcacaata
     1861 tcgacgtggt catcgaccgc gtgaaagtga aggcggacat caagcagcgg ctggcggaaa
     1921 gttttgaaac cgcgctgcgc cacggcaacg agcgtgcgct ggcgatggag atggacagcg
     1981 gcgaagaaca ttggttttcc gcacgtttcg cctgccccgt gtgttcgtac agcctgcccg
     2041 aattagagcc tcgccttttt tcgttcaaca acccgatggg ttcctgcccg acttgcgacg
     2101 gcttgggcaa caccaatttc ttcgaccccg aaaaagtggt cgcccatccc gaattgtcgc
     2161 tggcaacagg tgcgattgac ggttgggaca agcgcaacca attctatttc caaatgattc
     2221 aatcattggc gcggcattac ggtttcgatg tgcaggctgc ttgggaaaca ctacctgaaa
     2281 aagtgaaaaa agttgtgctg cacggctcag gcaaagaagt cattgatttc acttacctgt
     2341 ccgaacgcgg caccaccttc aaccgcagcc acgcctttga aggcatcatc cccaatctcg
     2401 aacgccgcta ccgcgaaacc gacagcgaaa ccgtgcgcga aaaactgcgc gaataccaaa
     2461 accaccgcgc ctgcccaagc tgcggcggcg cacgtttgcg caaagaagcc cgctacgttt
     2521 acgtcagcgg tgagccgttg cacgaagttt ccgcctggcc gctcaccaaa acccaccaat
     2581 tctttgaaac actggattta gacggcaaca aaaaacaaat cgccgaaaaa atcctcaaag
     2641 aaatcaccga acgcctcggc ttcctgatta acgtcgggct ggattacctg aatttaagcc
     2701 gctccgccga aaccctctcc ggcggcgaag cccagcgcat ccgcctcgcc agccaaatcg
     2761 gcagcggcct gaccggcgtg atgtatgtac tggacgaacc ctccatcggc ctgcaccagc
     2821 gtgacaatga ccgcctgctg gccaccctca aacgcctgcg cgatttgggc aacagcgtga
     2881 ttgtggtcga acacgacgaa gacgccatcc gcgaagccga tttcgtggtc gatatgggcc
     2941 ccggcgcagg cgaacacgga ggcaacgtac tgattgccga cacgcccgaa aacgtcgccc
     3001 aatgcgaaaa ctccgttacc ggacaatacc tcagcggcaa aaaatccatt gccgtgccgt
     3061 ctgaacgcac gcccgtcaat cccgaccgga tgctcgtcct caaaggcgca cgcggcaaca
     3121 atctcaaaaa cgttaccctt gagctgccgc tcggtttgat tacctgcatc accggcgtgt
     3181 ccggcagcgg caaatccacc ctgattaacg acaccctcgc caaaattacc gcccgcgaac
     3241 tcaaccgcgc ccaagaagaa cccgccccat tcgacgacat ccacggcctc gaacacctcg
     3301 acaaagtcat caacgtcgat cagtccccca tcggccgtac tcctcgctcc aaccccgcca
     3361 cctacaccgg cctgttcacc cctatccgcg aacttttcgc cggcgtcccc ctctctcgcg
     3421 aacgcggcta caacgtcggc agattctcct tcaatgtcaa aggcggccgc tgcgaagcct
     3481 gccaaggcga cggcgtgatt aaagtcgaaa tgcacttcct gcccgacgtg tacgtcccct
     3541 gcgaagtctg ccacggcaaa cgctacaacc gcgaaacgct cgaaatccaa tacaaaggca
     3601 aaaacatcag ccaagtcctc gacatgaccg tcgaagaagc ccgcgaattt ttcgacgccg
     3661 tccccaccgt atcgcgcaaa ctgcaaaccc tgatggacgt aggcctaggc tatatccgcc
     3721 tcggacagtc cgccaccacc ctctccggcg gcgaagccca acgcgtgaaa ctcgccttgg
     3781 aactctccaa acgcgacacc ggcagaacgc tctacatcct cgacgaaccc accaccggcc
     3841 tgcacttcgc cgacatcgcc ctgctgctgg aagtcatagg ccgtctgaaa ggcaaaggca
     3901 actcgatagt gattatcgag cataatctgg atgtgattaa aacagcggat tggattgtgg
     3961 acttggggcc ggaaggcgga gatggcgggg ggaggattat tgctaaaggt agtcctgaac
     4021 aggtggcgaa agttaagggg agttatactg ggaagtattt gaaggtggtt ttgaggtaag
     4081 tttaattcaa aattaaatag gagattatta agatgaaacc gttaaaaaca ttagaatttg
     4141 gatttgtgga tgctgcaaac tacagaagaa gagaaaataa agatttattt aaccgaatat
     4201 ttgtaaaagg agaatatttg gatgaattat gtgaaccaaa tatttcgttt ttaatcggag
     4261 aaaagggaac tggaaagaca gcatatgctg tttatttaac taataacttc tataaaaaca
     4321 tacatgccac tactaagttt gttcgtgaaa ccgattattc aaaatttatt cagctaaaga
     4381 aagcaagaca cttaactgtt tcagatttta caagtatttg gaaagtcatt ttatatctgt
     4441 tgatatcaaa tcaaatcaaa tgtaaagaaa acggaatatt atcttcaata tttaataaat
     4501 ttaaagcctt agatgaggct ataaatgaat attattatgg cgcttttgat ccggaaattg
     4561 tacaagcaat aactttaata gaaaattcaa aagaagctgc ggaaatgatt tttggaaaat
     4621 ttgttaaact aggtgaagag gaatcccaac aaataacttt tacagaaagt aaattccaag
     4681 caaatttagg ttttattgaa agaaaattta aagatgcttt atctcagtta aagctaaaag
     4741 ataatcatat tttgtttatt gatgggatag atattagacc atcacagatt ccatttgatg
     4801 aatatcatga gtgtgtaaaa ggtcttgcta acgccatatg gatgttaaat aatgatatct
     4861 tcccttccat taaagatagt aagggaagga tgagagttgt gttattgatt agacctgata
     4921 tctttgattc attaggttta caaaatcaaa ataccaaact tcaagataat tcagtatttt
     4981 tagactggag gacggattat aaatcttata gaagttcaaa gatttttggc gtttttgatc
     5041 atcttttgag aacccagcaa gaaaaacaag atagtttaga aaaaggcaac tcatgggatt
     5101 attattttcc atggaatgct cctaatttac atgatgagta taaaaattta acttcattta
     5161 ttagcttcct aagaaaatcg tattatcgac ctcgcgatat tcttcagatg cttactttgc
     5221 tacaaaaaaa taagaaaagt aaggaagatt atgtcgtagc agaagatttt gataatactt
     5281 cttttcaaag agaatactcg atatatttac ttggtgaaat caaagatcat cttttgtttt
     5341 attatagtca aagtgattat caaaatttcc tgaaattttt tgaattttta aacgggaaag
     5401 atagatttaa atatagtgat tttttaaaag catttgaacg tttgaaaaag cacttacaaa
     5461 caacatcagt ggaaatacct aaatttatga gtactgctaa tgagtttttg caatttttat
     5521 ttgacttgaa tgttattgct tatttagata acccagaaga tgaaacgaaa ccatatatcc
     5581 attggtgctt taaagataga aattatgcaa atatttctcc taaaataaaa actgaaactg
     5641 aatatttaat attttcagga ttatcaaaag cccttgatgt tggtactcca tttaagaaca
     5701 aacagtaact tgtatcccaa ccaaccgtgt gcgctacgca cacggttggt agctgacgtg
     5761 ttgcagtttc aacagagaaa gccgtagcct gcatacccaa ccgaccgcgt gcgtggctgt
     5821 gccacacgcc ctacctgtga attacacgga acttcaaagc caatacgaga gctttgcaaa
     5881 attcctttcc ctcccgacag ccgaaaccca aacacaggtt ttcggctgtt ttcgtttcaa
     5941 atatccacta attttacccc aataccccct taatcctccc aggatacccg ataatcaggc
     6001 atccgggccg ccttttaggc gcaaacaggc acacttagcc cgagaccttt gcaaaattcc
     6061 ccaaaatccc ctaaatgtct tggtgggaat ttagggaatt taggggattt tggggaattt
     6121 tgcaaaggtc tctgactgcc ccaaaagggc aaaaataatg tgggtatgtt gaggctgcct
     6181 ctttttgaaa tgacaaacaa aatgccgtct gaaacccgat ttccggtttc agacggcatt
     6241 tgatgttcaa tcagttgtca aacctgatta tgccaaacct tttgctttca acacttccag
     6301 catggtagtg cccaactcgg cagggctgcg ggtgtaagcg ataccggctt tttcgaaagc
     6361 ggcgaatttt tcttccgcag tacctttgcc gccggagata atcgcaccgg cgtgacccat
     6421 gcgtttgcct ttaggtgcgg taacaccggc gatatagccg acaacaggtt tgcttacgtt
     6481 ggattggatg tattcggctg cttcttcttc cgcagtaccg ccaatttcac cgatcatgat
     6541 gatggcgtcg gtatccgggt cttcttggaa aagtttcagt gcgtcgattt ggttcatacc
     6601 cggaatcggg tcgccgccga taccgataca ggttgattga cccaagccca gtttggtggt
     6661 ttgtgccacg gcttcgtaag tcaatgtacc ggaacgggaa atgatgccga tgcggccggg
     6721 agtatggatg tggcccggca taatgccgat tttgcactcg cccggagtaa tcacgcccgg
     6781 gcagttaggg ccgaccaaac gtgttccgtt accgttggtt tccaagtagc gtttggcttt
     6841 gagcatgtcc aaagtcggca cgccttcggt aatcacaacg accaagccta cgcctgaatc
     6901 aactgcttca acgatagaat ccaacacaaa cggagcagga acgtaaatca cggatgcatc
     6961 cgcgccggtt tctttaacgg cttctttcat ggtgttgaac acgggcaggt tcaggtgggt
     7021 ttgaccgcct ttgcccgggg taacgccgcc gacaacttta gtgccgtaag ccagagcttg
     7081 ttcggagtgg aaagtaccgt ttttaccggt gaaaccttga accaatactt tagtgtcttt
     7141 attaatcaat acgctcattc ttttctcctt aggcgtttac ggctgcaaca attttttcgg
     7201 ctgcgtcatt caggccgtct gcagaagtca gtttcagacc tgattcgttc aggattttcg
     7261 cgccgagttc ggcgttgttg ccttccaaac gaacaacgac aggaacgttg acgttgattt
     7321 ctttaacggc tgccacgatg gcttccgcaa tcatgtcgca acgtacgata ccgccgaaga
     7381 tgttgatcaa tacgccttga acggatttgt cttccagaat cagtttgaac gcttcaacca
     7441 cgcggtcttt ggttgcgccg ccgccaacgt ccaagaagtt ggcaggttgg ccgcctttca
     7501 gtttgatgat gtccatagtg gccatcgcca aaccggcacc gttcaccata cagccgatgt
     7561 tgccttccag ggcaacatag ttcaggtcaa attcagaagc tttcaactcg cgttcgtttt
     7621 cttgagattt gtcgcgcaat tcggcgattt tcggcaggcg gtagagcgcg ttgctgtcga
     7681 tgccgatttt gccgtccacg caggcgagcg cgccgttttc gcgaactgcc agcgggttga
     7741 cttcaaacag ggcgaagtca ttttcgacaa acgctttgta cgcaccggtc atcagtttga
     7801 cgaactcgtt gatttgtttg tctttcaagc ccagttggaa cgcaacttcg cgagcttggc
     7861 aaggttgcag gccgaccagc ggatcaacgg ttactttgaa gattttttca ggagtttcgg
     7921 cagcaacttt ttcgatttcc acgccgcctt cggtagaggc catgaatgta atgcggcggg
     7981 tagaacggtc aaccactgcg cccaagtaca gctcggtttg aaccggatac atgtcttcgc
     8041 aaaccaaaac actgttgaca ggttggccgt tggcatcggt ttggtaagtt accaagttgg
     8101 tgccaatcag gctttcagcc acttctttag cttcttcgcg gcttttaacg acttttacgc
     8161 cgcccgcttt accgcggccg ccggcgtgta cttgtgcttt gacaacagcg aatttgccgc
     8221 ccaatttgtc gtaagctgca gcggcttctt cgccgttgtg tgccaaaata ccgccttgta
     8281 cgggcaaacc gtagctagcc agcagttctt tagcctgata ctcgtgtaaa ttcatggatt
     8341 tctcctttag attagtggaa tgccccgagt ctgcctccaa atggcaagcc cgtgcgtttc
     8401 agactacctt tcggcggtcg gaaaatcgtc gtttcacttg ctgaaaggca accacatccg
     8461 cgccagcaga tacatggctg caccgcctcc cccgagaata aaaaagacga tgcctttttt
     8521 acgcgaattc ggacagagca gataaacggc aaggctgaaa ctgataaaca acaatgccac
     8581 gcgccaaacc aattctttat ctttgaaacg gttgagcgta taagtttcgg tcatcatcac
     8641 atacatctgc cacaatgcgg ccatacacat gctgatgatg cccgccggta taaagaaaat
     8701 accgactatt tctttgttca taatgaatgt aaatgctgtt tgtttgtctg acggaattat
     8761 aaatcagaaa acggagctct cggaacattc aatttgtcga aggccttaag gccgtctgaa
     8821 aaatttcaga cggccttaat gtctatcaac cgtgcaaagc gcgtttgtcg gccgccaatg
     8881 cagcttcgtg aaccacttcg gacaaggttg ggtgggcatg gataatgcgg gcgatgtctt
     8941 cgctgctggc gaagaattcg agcgcagtca cgccttcggt aaccaattcg ctgacaaccg
     9001 gaccaatcat gtgtacgccc aagatgcggt cggttttggc atctgccaac actttaaccg
     9061 tacctttggc tttgcccatt gccaatgcgc gaccattcgc accaaaacct gaagtacctt
     9121 ttttgtactc cacgccttcg gctttgagct gctcttcggt tttacccacc caagcgattt
     9181 caggatcggt gtaaatcacg aacggtacgt tgttgaagtc gatatgcggt ttttgaccgg
     9241 caatgcgttc ggcaacggca acgccttcgt cgctggcttt gtgtgccaac atcgggccgc
     9301 gaaccacgtc gccgattgcc catacgttag gcaggttggt acggcattcg ccatctactt
     9361 tgataaagcc gcgctcgtct ttttccaagc ctacggcttc cgcgttcagg cctttggtgt
     9421 ttggaatacg gccgatggca acgatcagtt tgtcgaatac ttcggttttg gcttcgccag
     9481 cagcagtttc gtaagcaacg gaaacacctt tgccttcaga cttgatgtcg ccgattttca
     9541 cgcccaattc gatgctcaga ccttgctctt tggtgaagta tttgaaggct tctttggcga
     9601 tttgttggtc ggcggcagcc aggaaagtcg gcgcggcttc aagaatggta acttccgcac
     9661 ccacgcggtt ccatacggaa cccatttcca aaccaatcac gccggaaccg atcacgccga
     9721 gtttggcagg tacttcggtc aggttcaatg caccttcgtt gtccaatacg ttcacattgt
     9781 cgatagcgac ttgtggcagc ggacgcggta cggaaccggt ggctacgatg acgtgtttgg
     9841 cttcgataac ggttttctcg cctttgttat cgacttcgat ttggtaagca tcgccatttt
     9901 taccggcaaa ggaagccgta ccgaacaggc tggttacttt atttttttgg aacaggaatt
     9961 tgacgccgcc ggtcagcttg gtcacgatgg catctttgcg ctcaatcatt ttggccgcgt
    10021 cgaattttac gtcgccgaca gtgataccgt gttcggcaaa ctcgtgttgc gcagcgtgga
    10081 aatgttcgct ggattgcaac agggctttag aagggataca gcctacgttc aagcaagtac
    10141 cgcccaatgc aggggcattg cctgctttgt taacgcctgc atcgacgcaa gcggttttga
    10201 aacccagttg cgcggcacgg atggcggcaa cgtatccacc cgggcctgca ccaatcacta
    10261 ctacatcata ttgagacatc gtgtcatcct ttgcttttaa cagtatctca aaataaaaac
    10321 gatacttttt gtttcttttt tattttgaat gactgcgttt tgatggtttt aagaaaaggc
    10381 cgtctgaaag tccatatctg ccgattcatt cgaatcaaat gtttcagacg gcctcaatcg
    10441 ttcgttgagc ctattgtagc acgatactac attacgctac atc
//
LOCUS       DAWGRZ010000056        10468 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000056 DAWGRZ010000000
VERSION     DAWGRZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10468
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.056"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(170..382)
                     /locus_tag="WHT44_001012"
     CDS             complement(170..382)
                     /locus_tag="WHT44_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ2533208.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
     gene            complement(394..675)
                     /locus_tag="WHT44_001013"
     CDS             complement(394..675)
                     /locus_tag="WHT44_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533209.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGRHYRHSENGITLYDFIFFDGKPADEARFAAILTETCRRAVKKICETK"
     gene            complement(713..976)
                     /locus_tag="WHT44_001014"
     CDS             complement(713..976)
                     /locus_tag="WHT44_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533210.1"
                     /translation="MADILPSDKRRQPPKTKGSTMNIIEQEMKHSSLRKIDAEIAKII
                     ADAHKINAESVKIAQESRWYPMIAATGLVTAIAAVLALIFKFA"
     gene            complement(996..1568)
                     /locus_tag="WHT44_001015"
     CDS             complement(996..1568)
                     /locus_tag="WHT44_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEZ2533211.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVL
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            1547..1858
                     /locus_tag="WHT44_001016"
     CDS             1547..1858
                     /locus_tag="WHT44_001016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533212.1"
                     /translation="MTSPVSPSIRAASIFPLARSISTACRTDINRLPLRLIASTKTAE
                     INLASSGLSSVLPASLRRLRVGLTLTASSLRYSVARLMPNSCACCLRYAERAPRSIAS
                     A"
     gene            complement(1880..2755)
                     /locus_tag="WHT44_001017"
     CDS             complement(1880..2755)
                     /locus_tag="WHT44_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ2533213.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELAEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHGISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2800..3090)
                     /locus_tag="WHT44_001018"
     CDS             complement(2800..3090)
                     /locus_tag="WHT44_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ2533214.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3102..3347)
                     /locus_tag="WHT44_001019"
     CDS             complement(3102..3347)
                     /locus_tag="WHT44_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ2533215.1"
                     /translation="MINHECINQPYGRILKFSRVQAKEMLTADELKENFAKNGQTLAQ
                     WARENGFKPRDVYLVIGGQRKGNYGKGHEIAKKLGLK"
     gene            complement(3340..3564)
                     /locus_tag="WHT44_001020"
     CDS             complement(3340..3564)
                     /locus_tag="WHT44_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ2533216.1"
                     /translation="MEKPLNFKPISYPQTRESAAGWFKRNGVCKAHWAKYFNLERTTV
                     EHLLRGKLKGNFGKSHEAAVLLGMKEKSDD"
     gene            3836..4207
                     /locus_tag="WHT44_001021"
     CDS             3836..4207
                     /locus_tag="WHT44_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ2533217.1"
                     /translation="MNNSSFFGNRLKEERKKLKMTQAEIAEKCGVSGRMWGDYERGIS
                     QPKAELFFQFEKVGIDVQYVMHGRRGETAVMPSETLNAEEQELLVLFREAAAADREMI
                     LMVARRAEKKAQTALGKVSNG"
     gene            4209..4832
                     /locus_tag="WHT44_001022"
     CDS             4209..4832
                     /locus_tag="WHT44_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533218.1"
                     /translation="MDQFELCQKEHVNPFALSKQYLLVVTFVKSSSKNFQAALLWARS
                     AKLFENLEIGKETIYCCAFDKTAEQAGMAGVFLNYIENWNGKQIYINGRIHSGSIYDL
                     LGVLDCYQKSQSCPNPKSHCCFVSDDIFLWHGSRPTFEISLDLTGKKKETSSAKKFVM
                     PCINFRHHRIEKETYLGNWNEQIAALAVKQNIDWCPSFDIENFRQYE"
     gene            4825..5019
                     /locus_tag="WHT44_001023"
     CDS             4825..5019
                     /locus_tag="WHT44_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ2533219.1"
                     /translation="MNNYYLYRNCSSDVLWVKRIQRQIDGSLLLISDNSTYPPMPLAL
                     AEHPDIQIIGQVVQVSKDLN"
     gene            5043..5606
                     /locus_tag="WHT44_001024"
     CDS             5043..5606
                     /locus_tag="WHT44_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533220.1"
                     /translation="MKILALLIAATCALSACGSQSEEQPASAQPQEQAQSELKTMPVS
                     YTDYQSAANKGLNDQKTGLTLPEHVVPIDNAEGKNLLHDFSDGLTILTVDTDKADKIT
                     AVRVVWNTDAMPQKAEKLSKAAAALIAATAPEDRTMLRDTGDQIEMAIDSHNAQKEPT
                     REWARGGIAYKVTVTNLPSVVLTAKAE"
     gene            5637..6584
                     /locus_tag="WHT44_001025"
     CDS             5637..6584
                     /locus_tag="WHT44_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533221.1"
                     /translation="MMIEKSISIVDGKEYSVFAVSHEFRYTFDEPILVADLISSLKAY
                     ETLTSSYLPAILNQLFDVKIQKIKVAVSEIERGSFLEKLIFNLFFKDEDAYNEFCLKI
                     RKFLGTENQDGSINMSKIIMFAMTTLLGVGAGYLLFKNPPQEKQAITNNIVTVINADS
                     SVALDGEHLVSVVKEVTGSSKQKTAENVAKVYAPASKNNGSITLGTDDVRIEPVAQQT
                     VATLPKDVDLRDTPLTEDYTDIDVQIRATDRDKNSGWYAVIDQIVPSRVRLELPEDID
                     LNRLANNATIRANVTVEFDLKQNGSRKPKKIILTSLSTD"
     gene            6669..7172
                     /locus_tag="WHT44_001026"
     CDS             6669..7172
                     /locus_tag="WHT44_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ2533222.1"
                     /translation="MSDKFNQFINRVLSHEGGYANHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAISIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGVIGAVSLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            7284..7616
                     /locus_tag="WHT44_001027"
                     /pseudo
     CDS             7284..7616
                     /locus_tag="WHT44_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7613..8023
                     /locus_tag="WHT44_001028"
     CDS             7613..8023
                     /locus_tag="WHT44_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ2533223.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAKQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMVALKKPDALIHGKEVRQKERNSSQG"
     gene            8056..8589
                     /locus_tag="WHT44_001029"
     CDS             8056..8589
                     /locus_tag="WHT44_001029"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ2533224.1"
                     /translation="MSFYYQLAVQSAEDKYADLKRHIHDIYRRHKGRYGYRRIAAAIR
                     HAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNILRRCFHAEKPNEKWV
                     TDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEILKGAPEKPGSSEKPI
                     LHSDQGWQYQMFFIKSS"
     gene            8613..8723
                     /locus_tag="WHT44_001030"
     CDS             8613..8723
                     /locus_tag="WHT44_001030"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ2533225.1"
                     /translation="MSRKGNCLDNAAMESFFGTLKSECFHTCKYDSVTES"
     gene            8730..8831
                     /locus_tag="WHT44_001031"
     CDS             8730..8831
                     /locus_tag="WHT44_001031"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ2533226.1"
                     /translation="MHEYIRYYNNDRIKLKLKGLSPVQYRTQSLKAA"
     gene            complement(9149..9511)
                     /gene="ssrA"
                     /locus_tag="WHT44_001032"
     tmRNA           complement(9149..9511)
                     /gene="ssrA"
                     /locus_tag="WHT44_001032"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(9574..10179)
                     /locus_tag="WHT44_001033"
     CDS             complement(9574..10179)
                     /locus_tag="WHT44_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ2533227.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QLSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
BASE COUNT         2552 a         2437 c         2655 g         2824 t
ORIGIN      
        1 gaaaacagct gaaaacctgt gtttgggttt cggctgtcgg gagggaaagg aattttgcaa
       61 aggtctcaga gtgagtttat tttggggcgg cggcaggtcg ggggcaagcg gcgtggggct
      121 tggttgtggt ttttaggttt ttgggggtaa aaaatgccgt ctgaactttt cagacggcgt
      181 ttgttttttc tatccaatcg aggaactgcc gccatttttc cagcggcata tcggcccgac
      241 gggtttcggt atcgggttcg gcttcccagc ggcctacctg tatgtagtgc ttcaccccga
      301 cgatttgcgc caactcggcc tgtgtcagtt tgcagcggtt gcgaagggtg cggaggttgt
      361 aaggcgtgta gccgagttcc atgtcgttgc ggtttatttt gtttcgcata tttttttgac
      421 tgcccggcgg caggtttcgg taaggatggc ggcaaatcgg gcttcgtctg ccggtttgcc
      481 gtcgaaaaaa ataaaatcgt aaagggtgat gccgttttcg gaatggcggt aatgcctgcc
      541 cgggcattcg ccgtctgtct gcctgtggat tttggcaatc aggcggggat agcggcaatg
      601 ggtaatgaaa cggcttgcgc cgtcttgccc gataatccat tcggggtagc ggttgaatag
      661 tgcggctctg ttcattttgt tcgtgggata aagcccctcg cggggcttgt ggtcaggcaa
      721 atttgaatat cagtgccaac acggcggcga tggcggtaac cagcccggtg gccgctatca
      781 tgggatacca gcgggactct tgggctattt ttacggattc ggcgttgatt ttgtgcgcgt
      841 cggcaatgat tttggcgatt tcggcatcta tttttctcag actggagtgt ttcatttctt
      901 gttcgattat gttcatcgta cttcctttcg tttttggcgg ttgccgccgc ttgtcggatg
      961 gtaggatgtc tgccatgtgt atatattgat accttttagg ttttattgca agtgttttgg
     1021 gcggcggctt cgtatgcttg gcggtggcgg cggctgtacc attcggcgag ttcgcgccgc
     1081 tcttggaggc ggtagctgtc ggcggtcagg tagtggtgca ggcttgagaa gccggcgcgt
     1141 tggagggcgg cgcggctgat gtggccgagg gcgaggtctg ttgtgagggt gtcttttccg
     1201 gcggtcaggc tgatgttggt gaagaggtgg cggaatccgt gcattgtgtg tttggatttg
     1261 ccgggggtgc tgccgtcata gcccagtcgt cggatggcgt tgtgggcgaa tttgatgctg
     1321 atgtggtcgg gatggggggc gggtttgcgc cgtgggcgga tgccggggaa aaggtggatg
     1381 ttgtcgccgg tctgtgtgtg cagctctcgg agtatttcta ccgcccagtc cgacagtagg
     1441 acggtaaagg ggtgtttggt cttcatgtcg gcggcgggga tgtgccataa ccgggcggtg
     1501 aggtcgatgt cttgccagcg ggcggaaagc agtgcggacg gacgggatga cttccccggt
     1561 ttccccatca atccgcgccg cctcgatttt tccgttggcg cgcagcattt ccaccgcctg
     1621 ccgcaccgac atcaaccgct tgccgttgcg cctcatcgcc tccaccaaaa ccgccgaaat
     1681 caatttggct tcttccggtt taagctccgt cttgcccgca tcgctgcgcc gtttgcgcgt
     1741 cggcttgacg ctgaccgcct ccagcttgcg gtatagcgtg gcaaggctga tgcccaattc
     1801 ctgcgcctgc tgcttaagat atgcagagcg tgcgccgcgt tccattgctt ccgcctgatt
     1861 ctcgactgcc ttaagacgct caatcattgc cggattcatc gccttctccc gtttcaccgc
     1921 ccaaccattc cggcacattg tctgtcggtg cttcagtcgg cagggcatag ctttcgcgca
     1981 gttgctcgca gtccaaaata atttgattga gcgtgccgac catctttgcc tgatggctga
     2041 tcccgtgtgc ctcactgtgc gcattaagtt ggtcgaacaa atctttcaga cggctcactt
     2101 gactgcggat accgacctca aggcttgtta actgcatcgc caactcgctg cctacgtctt
     2161 ccgccttcgg ctctctgaca acggtttgct tcttagccag cttctcggcc agctcatcaa
     2221 ttttggctgt tttggttttc atcacttcgt ctttggcggc gaggttttcg cggctttcgc
     2281 gcagggcgac gcgcagctcg cgcaccgtca ttcggtccac atcgtcaaag gtcatgccgt
     2341 tgacttcttc cccttcggcc aaacccacca gcgtaacgtc ttcttcgacc agcagctcaa
     2401 gcagcttcga cttgcccaaa tccatcagct tcggcgcggc tttctgcatt tgcggggtcg
     2461 caaagcggcg agtggctgac atcagacgtg atgtttctgc gatacccagc ccaaattggc
     2521 ttttcacaat ttccataaac cgtccgtgtt ccgtatgctc ttttaaaata atcagcgcac
     2581 gtcccagttc gaacatgcct tccatcgtct gccgtactgc ttgacgaccg cgttcaaccc
     2641 aacgctcttc gctataagtc tcgccgttac cccactgctc catcaccagc acactgcgca
     2701 tcgctgcctg attgcttact ttgtcggttg cgataatttc aatttctgta ttcatttttt
     2761 atctccaaag tttccgacgt cggaaacttt caaaatccgt taatccacat cgacccgctt
     2821 gccgatttcg gcaatcttgc tttgcagccg ttcatgctgc tgcctgaacc gctctgcgat
     2881 ttgcagggtt ttgatgccgt aggcgtagtt gccgttttca agtttgatga ccaatcccga
     2941 ggcaaccaaa tcatcaatat ccctgctgac ttgcgatggc gtcagcccca gtccgaccga
     3001 taaatcctta ttgctcagac cgataatcgg atgctcgtca agcgcgataa agaccctcaa
     3061 tagccgttgt acccttttac tttctgccat ccgcatcctc cttatttcag tcccagcttc
     3121 ttggcaattt cgtgcccctt gccgtaattg cctttgcgct gtccgccgat caccagatac
     3181 acatcgcgcg gcttaaagcc gttctctctt gcccactgcg ccagcgtctg gccgtttttg
     3241 gcaaaatttt ctttcaattc gtctgccgta agcatttctt tggcctgtac gcgggaaaat
     3301 ttcagtatcc gtccgtacgg ttggttaata cactcatgat taatcatcgc ttttctcctt
     3361 catccccagc aaaaccgccg cttcgtggct tttgccaaaa ttgcctttca gcttgccgcg
     3421 caagaggtgc tccaccgtgg tgcgctccag attgaaatat ttcgcccaat gcgccttgca
     3481 caccccgttg cgcttaaacc acccggccgc gctctcgcgc gtttgcggat aggaaatagg
     3541 cttgaaattc aaaggttttt ccatattatt tatgcctttc gtgtgatata atgtgtttgt
     3601 ttttaggtct ctttgcacga taaacatccg gtctgttgtg cagcagcagg gtcttggctt
     3661 gttttaaagt aaacaagctt tttctcgtag ctgggtgtat gtgatccatc atgagctgtc
     3721 gcgcatgagc cttcaaagtg ttcatttttt cgtcctttct cgtgatgatt tagggtgttt
     3781 gtgtttcgat gtggaaatta taggaagaat tcttcctatt ttgcaaggaa tatttatgaa
     3841 taactcttcc ttttttggca accgattgaa agaagaaaga aaaaaattaa aaatgactca
     3901 agctgaaatc gctgaaaaat gtggggtttc aggaagaatg tggggggatt atgaacgtgg
     3961 catcagccag ccaaaagcgg aacttttctt ccaatttgaa aaggtgggta tagacgttca
     4021 atacgtcatg cacggcagac gcggcgaaac agcggtcatg ccgtctgaaa ccctgaacgc
     4081 cgaagaacaa gaactgctgg tcttgttccg cgaggcggca gctgccgacc gtgaaatgat
     4141 tctgatggtt gcgcgcaggg cagagaaaaa agcccaaact gcgcttggta aagtgagtaa
     4201 tggataaaat ggatcaattc gaattgtgcc aaaaagaaca tgtcaatcca tttgccttgt
     4261 caaagcaata tctattggtt gtaacattcg tcaaatccag cagtaaaaat tttcaggcag
     4321 cattactttg ggcaagaagt gccaaattat ttgagaatct tgagattgga aaagaaacca
     4381 tctattgctg cgctttcgat aaaacagcag aacaggctgg gatggccggg gtatttttga
     4441 attatattga aaattggaat ggcaaacaga tttacatcaa tggccgaatc catagtggca
     4501 gtatttatga tttgttaggg gttttagact gctatcaaaa atcacagtcc tgtcccaacc
     4561 ctaaaagcca ctgttgcttt gtttcagacg acatttttct atggcatgga tcaagaccaa
     4621 cgtttgaaat cagtcttgat ctaactggaa agaaaaaaga aacatcctct gcaaagaaat
     4681 ttgtgatgcc ttgtattaat ttccgtcacc ataggattga aaaagaaacc tacttaggaa
     4741 attggaatga acaaattgcc gcattggcag taaaacaaaa tatagattgg tgtccaagtt
     4801 ttgatattga gaattttaga cagtatgaat aattactatc tatataggaa ttgcagcagc
     4861 gatgtgttat gggtcaaacg tatccaacgc caaatcgacg gcagcctact cttgatttct
     4921 gacaattcaa cctatccacc catgcccttg gcactggcgg aacaccccga tattcaaatc
     4981 atcgggcagg tagtgcaggt atcaaaagac ttgaactaga cacaatcaaa aagggaaata
     5041 gaatgaaaat actcgcttta ttaattgccg ctacctgtgc tttatctgcg tgtggcagcc
     5101 aatctgaaga acaaccggca tctgcacaac cccaagagca ggcacaatcc gaattaaaaa
     5161 ccatgccggt aagctatacc gactatcaat cagcagccaa taaagggctg aatgaccaaa
     5221 aaaccggtct gacccttcct gaacatgttg tccctatcga caatgcggaa ggaaagaatc
     5281 tgctgcatga cttttcagac ggcctcacaa tcttaaccgt tgataccgat aaagccgaca
     5341 aaattactgc tgtccgagta gtctggaata cagatgcaat gcctcaaaaa gcggaaaaac
     5401 tgtccaaagc tgccgcagcc ttgattgcgg caaccgctcc ggaagaccgc acaatgctgc
     5461 gtgataccgg cgaccaaatc gaaatggcga ttgacagcca taatgcgcaa aaagagccaa
     5521 cccgagaatg ggcgcgtggt gggattgctt ataaagtcac tgttaccaat ttaccgagcg
     5581 tggttttgac ggcaaaagct gagtaaatct attaagtaga aaaaatagaa agggaaatga
     5641 tgattgagaa aagtatttct attgtagatg gaaaggaata ctccgttttt gctgtatcac
     5701 acgagtttcg ttataccttt gatgagccta ttttagtcgc tgacttgatt agttctctaa
     5761 aagcttatga aacactgaca agtagttatc ttccagcaat tttgaatcag ctgtttgatg
     5821 tcaaaatcca aaaaatcaaa gtagctgtat ctgaaattga aagaggatct ttccttgaaa
     5881 aactgatttt caatttattc ttcaaagatg aagatgctta taatgaattt tgtcttaaaa
     5941 tacgaaaatt tctaggaaca gaaaatcagg acggaagtat taatatgtcc aaaatcatta
     6001 tgtttgcaat gactacactt ttaggggtag gtgctggtta tctcttgttt aaaaacccgc
     6061 cacaagagaa gcaggcaata accaacaaca tcgttaccgt cattaatgct gatagttctg
     6121 tcgcactgga tggtgaacat ttggtttcag tggtaaaaga agtaacagga agcagcaagc
     6181 aaaaaactgc agaaaatgtg gcaaaagtat atgctccagc aagtaaaaat aatggcagta
     6241 ttacccttgg gacagatgat gttcggattg aacctgttgc acaacaaact gtagcaactt
     6301 tgcctaaaga tgtggactta cgtgatacgc cattgactga agattacacc gatattgatg
     6361 tgcaaattcg tgctactgac cgtgataaaa attcagggtg gtatgcagtc atagaccaaa
     6421 ttgttccatc acgtgttcga ttagaactgc ctgaagatat tgatttgaat aggctggcta
     6481 acaatgctac tatccgtgca aatgtaacag ttgagtttga cttaaagcaa aatggctctc
     6541 gtaagcctaa aaaaatcatc ctcacatctc tctctactga ttaagtttta acccgtatta
     6601 aaggcttagt cagacggcct ttcctacaat ccctgtattg atttttaatt caatacaggg
     6661 atttttccat gtcagacaag ttcaaccaat tcatcaaccg cgtcctctct cacgagggtg
     6721 gttacgccaa ccatcccaaa gaccccggcg gcgaaaccaa ttggggcatc actaagcgca
     6781 ccgcacaggc aaacggctac aacggctcca tgcgtgccat gacgcgtgaa caggcaatca
     6841 gcatttaccg taaagcgttt tgggagcgtt accgcgccga ccaaatgccg gaagcggtcg
     6901 cgttccaatt ttttgatgcc tgcgtcaacc acggttacgg caatgccgcc cgtatgctgc
     6961 aacgcgccgc aggcgtaccg gacgacggcg ttatcggagc agtcagcctc aaagccatca
     7021 attcccttcc cgaaaacgac cttttattgc ggttcaacgc cgagcgtctg gtcttttata
     7081 ccaagctcgg tacgttcacc tctttcggca agggctgggt acgccgtgtg gcgcaaaacc
     7141 tgattcacgc gtctgcagat aacactgatt aaagggagat aaaccatgtc aaaaaagtca
     7201 ctcatcgccc taatgaccgc agccatgcag cccgatttca gccacagcga cctaggcatt
     7261 cgctacgcca tgccgactca gggatgttgg acgcaagccc accgcaagag cggggtagcc
     7321 gccgcgaaac gcgcagccaa aaaaaagcgt cacaaataac cgcctttttc cgatggctgg
     7381 gcggcttggt ctctaatccg gccacaggaa aaatcagcca taccaaacta tgggcaaacg
     7441 tcgccgcagc cgccatgact tggaagttcg tgcaggcggc ggacgcgccc gaatggctct
     7501 agtgggctta tggcgcattg gtcggcgggt atgcattaat caaacgcggc atcgcggcga
     7561 ttccgcagtt ggcagaaatc aaaaaatccg caaatcagga aggggggcgg caatgattga
     7621 atttgtccga gccaaaaaac ggctgctttg ggcatttgtg cttttgcttg tgtggacgtg
     7681 cggttaccga tacgccgccg acaaggccga agcgaaacaa accgccctga ttgccaccta
     7741 tcggcattct tctatggttg cggcggaaca atatgccttg cagcttaaaa aagcgcagga
     7801 cgaaaggcag cggtggtacg acttttccca aaaacaagga agaaagcccg tgaaaaaaca
     7861 gtatccgccg caaacgaaaa aagccggcta tctgaaaacc aaggaagaac tgcttgcgga
     7921 attggcttgc cttaaagcgg aaatggttgc cctaaaaaag cccgatgcct taatccatgg
     7981 gaaagaagtg cggcagaaag aacgcaactc gtcgcagggt taaggcaatg ccatccgttg
     8041 aactgctgtt ggagattgtc cttctattac caattggccg tccaatcggc agaagacaaa
     8101 tatgccgatt tgaaacggca tatccatgat atttatcgac gacataaggg aagatacggc
     8161 taccggagga ttgcggcagc catccgtcac gcaggaacac cggtcaatca caagaaagtc
     8221 agccgtctga tggcgaagac ggggctgaag gcagtgatac ggcggcgcaa ataccgctcg
     8281 ttcaaaggag aagtcggcaa aattgcgccg aatatcctgc gacgctgttt ccatgcagaa
     8341 aagccgaatg agaaatgggt aacggacgtt accgagttca atgtaggcgg agaaaagata
     8401 tacctttctc cgattatgga tttgtttaac ggggaaatcg tcagttaccg tattcaaatc
     8461 cgcccgactt tcgatttggc cggcgagata ctgaaaggtg cgccggagaa accgggatcg
     8521 tctgaaaagc cgatactgca ttcggatcaa ggttggcaat atcagatgtt ttttatcaaa
     8581 agcagttgaa aggcaacggt ctggttcaga gtatgtcccg caagggaaac tgcttggaca
     8641 atgcggcaat ggaaagtttc ttcggaacgt tgaaatcgga atgtttccat acgtgcaaat
     8701 atgattccgt taccgaatcg taagcggcac tgcacgaata tatccgttac tacaacaacg
     8761 atagaatcaa gttgaaatta aaaggactga gccctgttca gtacagaact cagtccctga
     8821 aagccgcttg attaaactgt ccgacttttt ggggtcagtt cggcttcggc atttttttat
     8881 ccgttttggg ggtaacttgt ttggaaagct gcaagcttat aaataaagga ttacatttaa
     8941 gttttgggta acctttttaa aaaaatgcgt gatgactttt gcatttttaa ggcgtttttt
     9001 ggggtaattc gtgaaaagtt accccaaaag ttaccccata aatggcgaaa actcaagcat
     9061 acgccagcat cctgcaacac aaaaaagcct tgaaactgtt gaagttcaag gcttttttgt
     9121 gttgcaggat gctgctgaaa atagggtatg gtggaggcgg ggggaatcga acccccgtcc
     9181 gaaagtcctc tacaaagcgt tctacatact tagttgtgtc tatttgaaaa tcttatttcc
     9241 atcatgccga ccaacaggcc ttttggaaac cagttacctt aagtcttatt tcctgccaag
     9301 taacccggta ggaaaccagt caatgtaaga tgacgttgcg gtggctttcg ccacacagcc
     9361 cattgaccga ctgctgcaac ggctagcctt aagcggctaa agcgtaagtt tcgtcgtttg
     9421 cgactatttg aattcagtgt tttacgggaa tctgagaccc cggtatgccc gcatctgctt
     9481 cgcaaccctc gtcgaaacca aggtcgcccc cagaaatggt ttgcaaatta tacggatatt
     9541 gtgcggtgct gccaagtctg tcggagaaat ttgtcagtct tgctgcctta atttgcgttt
     9601 gagcaggatg cggacgcagc cgtcgttgcc ttcccggggt tcggcgtagg cgagtacgtc
     9661 ggggtgttgc atcagccagt ttcgggtcat atttttcaga acgggtttgt agcctttgga
     9721 acctaatccg ctgccgtgga tgatttcgcc gcatacgccg cgtttttggg tgaatgcgat
     9781 gaattcgttg aggacttttt gggcttcttc ctgtgtgtag ccgtgcaggt cgacatcggt
     9841 aacgacggga tagtatccgt ttttcaggcg ttggatgtcg ttttttccct gtccgttttt
     9901 gctgaagctg gcgggcgggt cgttgtatgt gctgccgatg tagaagtagt tttcttcgtc
     9961 ggcgcggttg tctttgggac ggactttgat gggggttttg tcgggcggcg cataatattg
    10021 ctgccggttt tttaatgggg agagttgtcc gactgcttgt gaaaaatcga aatcctgttc
    10081 ttgtttttgc ttgttttttt cggctgcctg tttttctgcc gcttcttttt gggcttgttt
    10141 gcccagttgt ttgaggatgt tttggaagtc ggtattcata ttttttcctg ttatttgtcc
    10201 gatggctgtt tcgggcgggg ttttaatttg ccggaatgtt tgccaatcgg gggaggatga
    10261 ttttgttgcc tgcgtatgtt ttttgaaagt gtgattgtat atcaaaaaga aatgcggcaa
    10321 ccgtcggcag tgttgattgc cggaaatgcg gaccggtcga accgatatgc ccgaacgcct
    10381 gataaagttt taaaaacctg ccttgcgaag caggctgacg tgttttgcca atcttgaatt
    10441 gccggaaacg cgaaacacgg aaatctga
//
LOCUS       DAWGRZ010000057        10118 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000057 DAWGRZ010000000
VERSION     DAWGRZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10118
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.057"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            328..1032
                     /locus_tag="WHT44_001034"
     CDS             328..1032
                     /locus_tag="WHT44_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ2533228.1"
                     /translation="MLRLVLAASLSAVSFPAAAEALNYNIVEFSESAGVEVAQDTMSA
                     RFQVTAEGRDKNAVNAEFVKKFNKFIRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDELNRFIADIQADAALEYTDFHVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     PGVEEISISVNGTVQF"
     gene            complement(1096..1662)
                     /locus_tag="WHT44_001035"
     CDS             complement(1096..1662)
                     /locus_tag="WHT44_001035"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ2533229.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(1728..2144)
                     /locus_tag="WHT44_001036"
     CDS             complement(1728..2144)
                     /locus_tag="WHT44_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533230.1"
                     /translation="MLKHLAFLLPAMMFALPTSAAVLTSYQEPGCTYDGNVGKDGKPA
                     GKGTWRCQDGRNYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            complement(2206..3105)
                     /locus_tag="WHT44_001037"
     CDS             complement(2206..3105)
                     /locus_tag="WHT44_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ2533231.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            complement(3136..4593)
                     /gene="der"
                     /locus_tag="WHT44_001038"
     CDS             complement(3136..4593)
                     /gene="der"
                     /locus_tag="WHT44_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ2533232.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     EKFPEPEAEEADARHPVFAVIGRPNVGKSTLVNAILGEERVITFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(4743..5372)
                     /locus_tag="WHT44_001039"
     CDS             complement(4743..5372)
                     /locus_tag="WHT44_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ2533233.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RKVSQNQEAAAVLANIVEKAQSKAPQSEINAELTKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(5373..6668)
                     /gene="hisS"
                     /locus_tag="WHT44_001040"
     CDS             complement(5373..6668)
                     /gene="hisS"
                     /locus_tag="WHT44_001040"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ2533234.1"
                     /translation="MAQKIQSVKGMNDLLPVKQKDFKLTAAFWQAFEDTVGRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEDSQNHYVRFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTPTLQQWKNA"
     gene            complement(6762..7418)
                     /locus_tag="WHT44_001041"
     CDS             complement(6762..7418)
                     /locus_tag="WHT44_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ2533235.1"
                     /translation="MMKFKYVFLLACVVVSLSYRLNAAPMFNDNPVVYGKIKVQSWKE
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTEEEVLEKLDKEQMRASFEDMRRIMP
                     DLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGIDGNTVLLADPSLGHVSM
                     SRAQFLDAWQTREGNLAGKILAVIPKKAETISNKLFFTQHPKRQTEFTVGQIRQARAE
                     "
     gene            complement(7476..7667)
                     /locus_tag="WHT44_001042"
     CDS             complement(7476..7667)
                     /locus_tag="WHT44_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533236.1"
                     /translation="MLPTRSVSSLLLGEYLEFEENGTKIAAEIDSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
     gene            complement(7700..8236)
                     /locus_tag="WHT44_001043"
     CDS             complement(7700..8236)
                     /locus_tag="WHT44_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533237.1"
                     /translation="MKKIIDSKVRETNFNQAWRVIRAMQIDNISDEQVVIVRMSCYLK
                     KDEIFKNIERYGYQSSDIKKISPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKSGETLYSNFKSIKNQCKNYFFCGFDFDAPYFKSGV
                     SQVADYNFVPDAFSLFFI"
     gene            complement(8404..8604)
                     /locus_tag="WHT44_001044"
     CDS             complement(8404..8604)
                     /locus_tag="WHT44_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533238.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTRA
                     EVYINGRRVYHNEAEMASASLR"
     gene            complement(8719..9138)
                     /locus_tag="WHT44_001045"
     CDS             complement(8719..9138)
                     /locus_tag="WHT44_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533239.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGEWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMYSGLNLNQYGVASP"
     gene            complement(9196..9561)
                     /locus_tag="WHT44_001046"
     CDS             complement(9196..9561)
                     /locus_tag="WHT44_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533240.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(9543..10091)
                     /locus_tag="WHT44_001047"
     CDS             complement(9543..10091)
                     /locus_tag="WHT44_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533241.1"
                     /translation="MKKQITAAMMILTMIAAPAMANSMDNQIFENQVFHTRADAPMQL
                     AELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIP
                     GGVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPG
                     QGIGRHRPWESKSTDRSWKNRF"
BASE COUNT         2515 a         2402 c         2372 g         2829 t
ORIGIN      
        1 tgcaaaaccg taagggtcgg tcaggatgct tgctgtttct ttccgcaagt agcggaacat
       61 cggtgcaagc acggcggttt tgatgccttt cctctgcaat gccagtgcct gattttgaaa
      121 aaaatcccgt ccacatcctg ttcggattgc ggataccatg aggggatgac gaggacgtgc
      181 aagggttcgg gcatagtggg attccgtatc ggaaaggcgg ttattataag acagacgcag
      241 accgaatatt taaattgttg ccttacgcta atgcaatttg gcgcgccggt gtgttagatt
      301 ggcagtttta tcggtaagga ggcggatatg ttgcgtcttg ttttggcggc ttcgctgtcg
      361 gcggtatctt ttccggcagc ggctgaagca ttgaattaca atattgtcga attttccgaa
      421 tcggcgggtg tcgaggtggc tcaggataca atgtccgcac gtttccaagt gacggcggaa
      481 ggacgggaca aaaatgccgt caatgctgag tttgttaaaa aattcaacaa gttcatcaga
      541 aaatcgaaaa atggtagctt taaaaccgaa ttggtatcgc gcagtgcgat gccgcgctat
      601 caatatacca acggcagacg cattcaaaca ggctgggagg agcgtgcgga atttaaggtc
      661 gaaggtagag attttgatga gttaaaccgt tttattgccg atattcaagc agatgccgcg
      721 ttggaatata cggatttcca tgtgtcgcgc gaacgccgca acgaggtcat cgatcaggtc
      781 agcaaggatg ccgttttgcg tttcaaggcg cgtgccgaaa agttggcggg cgttttgggt
      841 gcgtccggtt ataaaatcgt caaattgaat ttgggacaca tcggcagcca tatcgcggga
      901 gggggagctg ctcaggcaaa aatgcttcgt gccatgccga tggcggcaag cgtcaatatg
      961 gagggtgcgg attccgccgc gcctggtgtg gaggaaatca gcatcagcgt caatgggacg
     1021 gttcagttct gatttgaggt gaacggcaaa tgccgtctga aacccgacga taagggttca
     1081 gacggcattt atatttcagg ctttgggcag ggtaacgccg gtttgcccca tatatttgcc
     1141 gttgcggtct ttgtatgagg tttcgcagat ttcgtcgctc tcgaagaaga ggacttgcgc
     1201 cacgccttcg cctgcgtaga ttttggcggg caggggggtg gtgttggaaa attcgagggt
     1261 aacgtagcct tcccattccg gttcgaacgg ggtaacgttg acgataatgc cgcagcgggc
     1321 gtaggtggat ttgcccaagc agacggtcag gacgttgcgc gggatgcgga aatattcgac
     1381 cgtgcgcgcc agtgcgaagg aattgggcgg gatgatgcag cagtcgtctt caacggtaac
     1441 gaagtttttc gggtcgaagt ttttgggatc gacgatggtg ctgttgatgt tggtgaagat
     1501 tttaaattcg tttgcgcagc ggatgtcgta gccgtagctg gacgtaccgt aggagatgat
     1561 gcgtttgccg tcggcttctt tgatttggtt cggctcgaaa gggtcgatca tgccgaattc
     1621 ttcgctcatt cggcgtatcc atttgtcgga cttgatgctc ataatgtttt ccttgtttct
     1681 tgcagtgttc ggacaaagca ttgggggatg ccgtctgaaa acggggctta tttgtttttg
     1741 ggcagtttca cttctttaat catgccgttt tcgcatttca taatgaagag ggtttcgccg
     1801 ttttgcgaga cggtaaatct gccgtgtgcc aagccttttt tgaacgtgcc cgagagtacc
     1861 atgttgcgga atttggtact gtcggaattg aacggttcga taaatatttc gcggttggcg
     1921 gcaacggtat aaacgccttg cccgtcgaat ttgccgtttt taaacgaacc ggtatagttg
     1981 cgcccgtctt ggcagcgcca tgtgcctttg ccggcgggtt taccgtcttt gccgacattg
     2041 ccgtcgtagg tgcagcctgg ttcttgatag gaagtcagga cggcggccga agtggggagg
     2101 gcgaacatca tggcgggcag taggaatgcg agatgtttaa gcataagggt tattccattg
     2161 gattttggtt gacggtattt tgtcgtgaaa aagccgtctg aaaaatcaat cttgccagcc
     2221 gcccaaatag gaaaccagtt cttccaacat ggtgctgacg gattccgcca tcagaatttg
     2281 cgaggcgaag gcaaggccgg cggcatcgtc gccgttgctt tcggcttctt cctgcaatac
     2341 gtcgaggtat tggatgcgct tgagtgtgaa gtcttgagtg aggataaagg caatttgttc
     2401 gcgccacacc aagcccagtt gggtaacggt tttaccgttt ttgacgtgtt gaaccacttc
     2461 gtcggcggtt aaatcttgtt tggatacttt gacgacggga acaatatcgc ccgtaccttt
     2521 gagttcgcaa tcgctgtcta attcaaaacc gccttcgcaa tgcccttgca acagccagcc
     2581 ggtcatcaag gaagagggcg attgcttggt attcggcagc gaggcttcca aaccgcccaa
     2641 agcttcgcgc agcttggtca ggatgttttc tgctttggcg gaagccgcgt tattgacgag
     2701 caggtagccg tggcgggtgt taaacaccgc ttctgtacgg ctgctgcggg taaacgctcg
     2761 gggcagcagg tcgtctgtaa tttgctcttt aagctcttgt ttttctttac ggccgacatt
     2821 gcgggcttca ttgttttgga tttccgctac cttctcttcc aaaatatcgc ggatgacgcc
     2881 ggcaggcagg actttttctt cttttttcag ggcgacgcgc aaggtaaagt cggcagggaa
     2941 aacgagttca ggggagaatg aaaccggtgc ggtaaagcct tcgctgaacc agtctaagcc
     3001 ttggcaatgg gtaaattcag cttcagcaag tttgtcggca agtacgtctg cctcaggcag
     3061 cttttctttg ttgagcggat aaaaactaat ctgcttgaac cacataatgt ttcctattgt
     3121 ttgaaatgtc gggaattatt tgctgaattg ttttttcaca ctgactttgg ttttcttctt
     3181 gaagcggttt ttctcttcct ttcggccttc gcgtttctca atacggttgc tcaggctgac
     3241 gcggcgcagc ggttttttct tcggtttgtc ttccgcattt tcatacgggt tttccgaaac
     3301 attgtattga attctgagcg gcgtgccttg cagattgaag gctttgcgga acgtttgggt
     3361 cagatagcgc gtatagctgt cggaaatcgc gtgcagcgaa ttgccgtgta ccacaattac
     3421 gggagggttc atgccgcctt ggtgggcata acgcattttc ggacgcacca agccggcacg
     3481 cggcggttgc tgacgctcga tcgcgctttg taatacgcgc gtgattttcg gcgtcggcat
     3541 cttaatcatc gccgcgttgt aggcagcctg aatgctgtca aacaaaccgt ctataccgcg
     3601 ctctttcaat gcggaaataa agtggaactt ggcaaaatcg aggaaataca gtttgcggtt
     3661 gatatcgcgt ttcacttgct cgcgacgttc ttcgctgatg ccgtcccatt tattgaccgc
     3721 caccaccaaa gcacggcctg cctccaaagc gaaacctgca atcgtcgcat cttggtcggc
     3781 gatgtcctgc tgcgcgtcca ataccaaaac agcgacgttt gccgcttcaa ccgcctgcat
     3841 cgctttgata acggagaatt tttccactgc ttcatccact ttgccgcgac ggcgcacacc
     3901 tgcggtatcg atgatggtaa acggtttgcc ttcgcgctcg aaatcgatat ggatactgtc
     3961 gcgcgtcgta cctgccatat cgaaggtgat gacgcgctct tcgccgagaa tggcgttaac
     4021 cagcgtagat ttgccgacgt ttggacgacc gataacggca aaaacaggat gtcttgcatc
     4081 ggcttcttcg gcttccggct cggggaattt ttccaaaata tcttcaatca gataatacac
     4141 accatcgccg tgtgcacctg aaataacata agggtcgccc aaagcaagtt cgtagaactc
     4201 ggcggcaagt acagccctat tgcccccctc gcctttattc acggccaaat aaacagggcg
     4261 cggactttgg cgcaaacggt cggcaataat cttgtcttgc ggtgttaaac cggtacggcc
     4321 gtccaccaaa aacacaactg catcagcttc atcgacagcc tgtaaggttt gttttgccat
     4381 ttcgtgcaaa atgccgctgt ccacaaccgg ctcgaaaccg ccggtatcga tgaccaaata
     4441 aggtttgctg ccgactttgc cgtgtccgta atggcggtcg cgcgtcagac cgggcaggtc
     4501 atgcacgagc gcgtctttgg tgcgcgtcaa acggttgaat aaagtagatt tgccgacgtt
     4561 ggggcgacca acaagcgcga tggttggttt catgattgag tctttctgtg tcaagtgccg
     4621 ttcgggagaa ctgaacacga gcaggtgtcc gttggacacg gcggatgggt tttacgggaa
     4681 ttgccgtagg atagtgttgt ctgaaatgcc gtctgaagag agggtggcat ttcagacggc
     4741 atttatttca gcgaatcaag tttcatttga accaattcgc gaccgacaga atcttgaggc
     4801 attttttcta aagcctgtcc gtagtttttt aaggcttcct ggctttttcc ctgtgcggca
     4861 tagacatcgc ctttggtttc catcagcagg ggggcgaagt ccgcttcaac cggcgtatcg
     4921 agcgcggcaa gcgcggcatc gtattttttt tgttgcaaca acacaacgcc cagacgctgc
     4981 gccgccaacg cttgaatcag gctgtctttt tggttggaca acacccattt caaatggcct
     5041 tcggcaacat cgtaacgctg cgcgtcaaat tcggttgccg ccgccatcag tgtggcttgg
     5101 gcggcggaaa tggaatgcgg gtagctttgt tggagtttgg tcaattcggc attgatttcg
     5161 ctttgcgggg ctttgctttg cgccttttct acgatgtttg ccagcaccgc cgccgcttcc
     5221 tgattttggg aaactttacg gttttggtaa accgtgtatc ccaagtagcc gagtgccgcc
     5281 aaaatcagca aggcaaacag ccatttgccc gtggttttcc aaaaatattt aaagttgtct
     5341 aactcttgtt gttcttcgag atgggctgcc atttatgcgt tcttccattg ttgtaaagta
     5401 ggggttaaat cctcggcggc gacagtttgc tgaccgtgtg cgccgttcat gtctttgagc
     5461 gtaaccgtac cgttcgccag ttcgtcttgc gcgacaatca gggcaaagcg tgcgccgctg
     5521 ttgtcggctt ttttcatttg cgctttcagg ctttgatagc cggaatgctg cattacattg
     5581 aaaccttgcg cgcgcaaggc ttgtgcgtat ttcatcacct gcaagtccgc cccttcgcct
     5641 tggtgcattg catagacatc aggcgcagcg ttcacttcca gagagccgta ttcgctcacc
     5701 aaaagcagca gccgctcgat acccattgca aagccgatag acggcgcagg tttgccgccg
     5761 agttcttcaa tcaagccatc gtaacggccg ccgccgcaca cagtcgcctg cgcgccgagt
     5821 ttgtcggtcg tccactcaaa aaccgtctga ttgtaataat ccaaaccgcg aaccaagcgc
     5881 ggattttcaa tatattggat acccaaacca tccaacatcg ccttgaagcg tacatagtgg
     5941 ttttgcgaat cctcgcccaa gtaatccacc aaacgcggcg ccgcgttgca gatttcctgc
     6001 aaatctgggt ttttcgtatc caaaacgcgc aaaggattgg ttttcagacg gcgtttgctg
     6061 tcttcatcca atttatcttc ataacgggtc agatattcaa ccaatgccgc acggtgtgcc
     6121 gcgcgttcct cacggttgcc caagctgttg atttccaaag tcaggtattc gcggataccc
     6181 aatttttccc ataagtcggc agacatcgcg atgatttccg catcaatatc cggcccttca
     6241 aaacccaaag cctcgatacc gacctgatgg aactgacgat aacggccttt ttgcggacgc
     6301 tcgcggcgga acatcggccc catataccac agcttttgcg ggctattgta cagaaggttg
     6361 tgttcgacca ccgcacgcag gcaggaagcc gtaccttcag gacgcaagct caaactcaaa
     6421 gaatcgtttg aatcggagaa ggtgtacatt tccttgccga ccacatcggt ttcctcgccg
     6481 atggagcgga caaacaaacc ggtttgctcg acaatcggcg tacggatttg ctgataaccg
     6541 taagcgcgtg tccagcggcc gaccgtatct tcaaacgcct gccaaaacgc agccgtcagt
     6601 ttgaaatctt tttgcttgac aggcagaagg tcgttcatgc ctttgacgga ttggattttt
     6661 tgtgccattt caagtaagaa tgcttaaatc aaattgcggg cgattatagc ggattttaaa
     6721 gggtttgtga ggttggaggt ggtttgcgga cggcatttga cttactctgc acgtgcttgc
     6781 ctgatttgtc cgactgtaaa ctccgtctgc cgttttgggt gttgtgtgaa aaacaattta
     6841 tttgaaattg tctcggcttt tttcggtatg acagccaaaa tcttacctgc caaatttccc
     6901 tcacgggttt gccaagcatc caaaaactgc gccctgctca ttgaaacatg ccccagcgac
     6961 gggtcggcaa gcaaaaccgt attgccgtct ataccgcgca ataccgaaaa atggtcgtct
     7021 ttgcggtatt tcagatacac gatgacgggg attttcaact gcgcgagctg ctcgaaagac
     7081 agggcatagc ccttcgcctc aaaacccaaa tcaggcataa tgcgccgcat atcctcaaac
     7141 gacgcgcgca tctgctcctt atccagtttt tccaacactt cttcttccgt cagcttttgc
     7201 ccgtaaaaat tgttcaaaag tgtcgccacc gaagccgccc cgcaggaaaa atccaaatcc
     7261 tgctttacaa tattgaaatc ccgcctttct ttccaactct gcactttgat ttttccgtaa
     7321 acaacaggat tatcgttaaa catcggtgca gcattcaaac gataagataa agaaacgaca
     7381 acacacgcca acagaaaaac atatttgaac ttcatcatat tgtccacata aagggcagcc
     7441 tgaaaatctt tcaggctgcc cttgtcaaat tattcctagc tttcggcttt tttggcaaac
     7501 caaacaatcc gattacccgc ataatacttt ccatttattg aaatccgaca agccgcgccc
     7561 aaaaaatgcc atgcactgtc gatttccgca gcaatctttg taccgttttc ttcaaattcc
     7621 aaatattcac ccaataataa acttgaaaca gaacgcgtgg gcagcaagtg cccaccctac
     7681 gcttattcaa ataatttgat taaataaaga ataaagaaaa tgcatcagga acaaaattat
     7741 aatctgccac ctgactcaca ccgcttttaa agtaaggggc atcaaaatca aaaccgcaaa
     7801 aaaaataatt tttgcattga tttttaatag atttaaaatt cgaatatagt gtttctccag
     7861 atttaaaaag tttatcttta tctatatatt tgatgctttc cctatccaaa ataaatattt
     7921 caaacattaa aaaatcatta catgaccaag ccaaatcata aatttgattc aaatgggtat
     7981 cataaagata aaaataataa ggtttgggaa caggtaaaat atttaatgga aaaggaggac
     8041 taattttctt aatatctgat gactgatatc catatctttc tatattctta aaaatttcat
     8101 ctttcttaag ataacaagac attctgacaa tcacaacctg ttcatcggat atattatcta
     8161 tttgcatggc gcgtataaca cgccatgcct gattaaaatt agtctccctt accttagaat
     8221 ctattatctt tttcaaagat gaaaatgccc cttatcttga actctccagt tagaacctgg
     8281 aagtttcgta cctcttaaat gtgtattaat atttcttata gtttcattaa aatgccacgc
     8341 gctgccgatt tcaacggtaa ttttcgtacc gctttcttca aattccaggt attcccccat
     8401 tagctaacgc aaagaagcag acgccatttc ggcttcgtta tgataaaccc gccttccgtt
     8461 gatatagact tccgcccttg tccgctccaa attccaaaaa ttccgtactg aaatttccat
     8521 atcccgatat tgtgcagacc atgttttttc gaaggttttc ataaaatttc ctatacctgt
     8581 ccaatcggca catatcaatt gcattattat agtggattaa caaaaatcag gacaaggcaa
     8641 cgaagccgca gacagtacaa atagtacaga accgatttac ttggtgtttc agcaccttag
     8701 agaatcgttc tctttgagct aaggtgaggc aacgccgtac tggtttaaat ttaatccgct
     8761 atacatctca atacgataaa tattccttaa gtcaaaatgc aagcctgacc gtaccttaac
     8821 tgtcaaaatt ttattatttt ttattgattt taaaacaatt tctgcaaaat tctcttcgct
     8881 ttctctcttt tttagaagca cataagaaaa aataaaactt ccccgattaa attcataaat
     8941 atgtttcaac cattcgcctc ctctttctgt aagacaagat tcagtttcat tcttccttat
     9001 tgtataaata tttccttcac aaaatctgaa ataaatccat aaatccatct tatccataat
     9061 taaagaaaaa gtttcacctc gagattttgt caacaattcg caaggttgcg atgttgcaat
     9121 caaatagccg aaagacattt tttacctcat acatggtcga aatcagtttc tgttagttca
     9181 gaatccattt tttcgtcaac aactgaatcc gcatttttga attaacgttt tcatcagctg
     9241 ccgtttatct aaaccggcag gttcagtttc aaaataagcc ttatatgaag actgtaagca
     9301 tttcagaaaa agatcatcag aagacatatc tgccgaatca aatacaactg ttttgatttt
     9361 ggtacttacc caaaaccctt tttgctcttt ttctactata cggaaattca gaatatttcc
     9421 aaccgaatca aaagcacggt aaacatcatc catcaaatcc tgcggctcta ttttcttttc
     9481 caattccgac aatccttgaa atatatccaa agacacatct tcaaatagaa aaaaaggagg
     9541 agttagaagc ggtttttcca tgatctgtcc gtagattttg attcccaagg gcgatgacga
     9601 ccaattccct gtccaggcaa agtcttgccc gtattatccg taactcgacg atgataatgg
     9661 ggaaattttc caataggatg acctgttcta ttaccgaaag gggctatccg catattattg
     9721 ccgattttaa tctcacgtcc atatttagca aaggaaacaa cctttcctgc ggcgcctaca
     9781 ccaccaggaa ttgcgcctaa tccgccagca atagcaacat ctctaacaga agctggtctg
     9841 cctgtcgttg cataactaaa accatgctgt gtccacatac caatggcagc accacccaag
     9901 atagccaatg gaagaaacgc cccctcagtc tccttcatct ccttctgaga aagctccgcc
     9961 aactgcatcg gcgcatctgc ccgcgtatgg aatacttggt tttcaaatat ctgattgtcc
    10021 atgctgtttg ccatcgccgg agcggcaatc attgtcagaa tcatcatcgc tgcggtaatt
    10081 tgttttttca taataactcc tttggattac aaggttgg
//
LOCUS       DAWGRZ010000058        10087 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000058 DAWGRZ010000000
VERSION     DAWGRZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.058"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(109..1782)
                     /gene="recN"
                     /locus_tag="WHT44_001048"
     CDS             complement(109..1782)
                     /gene="recN"
                     /locus_tag="WHT44_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ2533242.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSREQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRLLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(1966..2568)
                     /locus_tag="WHT44_001049"
     CDS             complement(1966..2568)
                     /locus_tag="WHT44_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ2533243.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLGLN
                     SLEELPPLTELGELVLPDLIEMPPTDEEEPETVPSDTLPN"
     gene            complement(2669..3610)
                     /gene="ylqF"
                     /locus_tag="WHT44_001050"
     CDS             complement(2669..3610)
                     /gene="ylqF"
                     /locus_tag="WHT44_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ2533244.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKIWLEHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEEGGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3691..4545)
                     /gene="rapZ"
                     /locus_tag="WHT44_001051"
     CDS             complement(3691..4545)
                     /gene="rapZ"
                     /locus_tag="WHT44_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ2533245.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            complement(4526..5488)
                     /locus_tag="WHT44_001052"
     CDS             complement(4526..5488)
                     /locus_tag="WHT44_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ2533246.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQHNEDRPD"
     gene            complement(5490..5939)
                     /gene="ptsN"
                     /locus_tag="WHT44_001053"
     CDS             complement(5490..5939)
                     /gene="ptsN"
                     /locus_tag="WHT44_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ2533247.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLIFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEVRAILTEE"
     gene            complement(6124..7014)
                     /gene="ubiA"
                     /locus_tag="WHT44_001054"
     CDS             complement(6124..7014)
                     /gene="ubiA"
                     /locus_tag="WHT44_001054"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ2533248.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            complement(7044..7520)
                     /locus_tag="WHT44_001055"
     CDS             complement(7044..7520)
                     /locus_tag="WHT44_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ2533249.1"
                     /translation="MEHLFGKWLPDLPAAISDGISLPMVRLLHTRSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDRIPVVEARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVAGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7535..8182)
                     /gene="bioD"
                     /locus_tag="WHT44_001056"
     CDS             complement(7535..8182)
                     /gene="bioD"
                     /locus_tag="WHT44_001056"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ2533250.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGIPMQEADKRRLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLVFNHIHDSRDAHIAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            complement(8179..9480)
                     /gene="bioA"
                     /locus_tag="WHT44_001057"
     CDS             complement(8179..9480)
                     /gene="bioA"
                     /locus_tag="WHT44_001057"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ2533251.1"
                     /translation="MPSEHQHISSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMAHVMFGGLTHEPAVELGKLLV
                     GILPQGLNRIFYADSGSISVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKSRFDDEWDGADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLHDLCDEFDIMLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            9697..9812
                     /locus_tag="WHT44_001058"
                     /pseudo
     CDS             9697..9812
                     /locus_tag="WHT44_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         2333 a         2747 c         2569 g         2438 t
ORIGIN      
        1 gcacaatcac ttgttcaagt gattaagaaa gcgtgataat acggatttat ctgagttctg
       61 tctattgttt tagactttta tttttagtta ttttttaact gattgatttt aaaataaact
      121 atttttcgac gcaagttgca gcaattctgc cgcatgttgc cgcgtcgtat cggtaatgac
      181 ttctccgccc aacatacggg caacctcttc gatccgttgg atttcatcca atatactgat
      241 ttcgctgacg gtttgctctc cctcgctgtg cttgcgcacc cgccagtggt tttctccgca
      301 ggatgcgact tggggaaggt gggtaacggc aagcacctga tgttttctgc ccaacgcacg
      361 taatgccttg ccgaccattt cagccactcc ccctccaata ccggtatcga cctcatcaaa
      421 aatcagggtg ggaacttggg tatattggct ggcaacaacc tgtaaggcaa ggctgatacg
      481 cgccaattcg ccgccggagg caactttatt cagcagacgg ggcggattgc ctttgttggc
      541 ggcaacttga aattgaacct gctccaaacc gtgtgccgtc ggcgacgaag gcaacaggac
      601 gatgtcgaaa cgcgcgcctt tcatggcaag gtgttgcata tgctcggtcg tttcgccgct
      661 caaacgctct gccgcctgat ggcgcatggc agaaaggatg tgggcagctt cctgatattc
      721 ggcaaaattg tgggcaacat tatgctcgag cgcgtccaaa tcggcggcag cttgcaggct
      781 ttgcaggcgt tcttcgattt ctgccaactt ggcaggcaac tcttcaggct cgatccggta
      841 tttccgcgcc atccccatca gctcgcccat gcgctgctct tgtgcggcaa gttcgttggg
      901 attgatgtcg ctgcgacctg ccacatcgcg catattggca ctgatttcgc ccaattcggc
      961 ttcgatgctt gccaacatat tcaggctctc ggcaaagcgc ggctcgatgt tttgcagatt
     1021 ggccaatagt ttttgacatt gatagatatg gcgttggatg ccgttgtcgc cgtcaatctt
     1081 gcttccgact tcttcggcag cctgcaacag ctcggcagaa tgggcaaggc tgtcgtggct
     1141 ttggctgagg gcttcccact cgccttgttt aatgtccaac tgattcaatt cgttaaactg
     1201 ccattccaga cgctcccgct cgataatgac ggcatcggcg tgttcctgcg cctcttggag
     1261 ggcttttttc gcattggccc aattttgata aagctgcctg acggtttccg cctgctccct
     1321 gctacccgca aatgcgtcca acaattcgcg ctgggcggct tcctgattaa gcgaatgatg
     1381 ggcgttttgc ccgtggatgt cgataagctg gctaccgacg gctttgagtt gcgccaaggt
     1441 agcggcctga ttgttgataa agctgcggct tttgcctttg gcatcgataa tgcggcggat
     1501 actgagttct tcttctccgt cgtttaaaag cccctgttca tacaattctg cttttaaaac
     1561 aggtaaatgg gaaatatcaa acaacgccga caactgcgct tcttttgcgc cgctgcggac
     1621 ttggctgtaa tcggctttat cgcccaacag cagaccaatc gcatccaaag taatggactt
     1681 gcccgcgcca gtttctccgg tcaatacggt aaagccgctt tgaaaatcca gattcagatt
     1741 ttcaacaatg acaaaatcac gcaaagaaag tgttagaagc atcgtgagga ttccttagca
     1801 attctaccga caaaagcaat atcaacttta gatatacgaa ttataactaa gattttttaa
     1861 attaaaaata agtttttcct taatttttct tagtgcttgt ttttatctgc ttatttgttg
     1921 caatgccgtc tgaagcaatg tgcgtttcag acggcatttg gaatttcagt tgggcagggt
     1981 atcggacggt acggtttccg gctcttcttc atccgtaggc ggcatttcta tcaaatcggg
     2041 caaaaccagt tcgcccagtt cggtcagcgg cggcagttct tccaagctgt tcaaacccaa
     2101 atcgctgagg aacgttgccg ttgtcgccca caatgcgggt tttcccaatg tgtcccgatg
     2161 tccgatgact tcaatccacc cccgatcctg caaggtctgc atcacgttct gcgacaccgc
     2221 cacgccgcgt atgccctcga tgtcgccgcg cgttacgggc tgctggtagg cgataatcgc
     2281 cagtgtttcc atcacggcgc gggagtagcg cggcgcacgc tgttcttgca ggctgcccag
     2341 ccgctcgaat gccgtctgaa caatctgaaa acgccagccc tcttgcgtat gcaccagttg
     2401 caacgcccta tcctgccaac gcgttttcaa ctgcgccaac acatcaatca gtttgtcttg
     2461 cgacaacggc ggcacacaca gttcgcgcat agatttttcg gtcagcggtt cggtttgggt
     2521 cagcagtgcg gcttcaatca gcgcgtcggg agaaattttg tcggtcatac gggtatccgt
     2581 gctgaaacgg catgggtttg gatatgccgt ctgaaatcgg ttggagtaga gagaagctgc
     2641 ctgaaaaata tttttcagac ggcattcttt atgcttccga aggcttctgc cctttacgct
     2701 ctgcttttct ggcttcgcgt atggctttga gttcggcttc tttctgacgg gcttttttga
     2761 gccaagtttc ccattggttc ggcgtttcga gggtgattct gccgattttg ccttcacgga
     2821 agtcggtgag gatgttttcg gcggcttttt ggtagttgat ccgtccgccg ctgaggactg
     2881 cgccgcgttt tttggctatc cattcgagcc aaacgttttc gtcccagtgg ctgctggggt
     2941 ctttgtcggc ttggtagcgt tcttgcaaca tagggaggta gtggcggcgg aggtagtcta
     3001 aaagttcgag ggcgacttct tcttcgtcca acgcgttgcg tccgactgcg ccgccggcgg
     3061 caaggttgta gccgccttct tcgacgatga ttttcggcca tagcattccg ggggtgtcgt
     3121 agagccagaa gtcatcggcg aggaagaggc gttgttcggc tttggtgatg ccgggttcgt
     3181 tgccggtttt ggcggatttt ttgcctatca tgccgttgat gagggtggac ttgccaacgt
     3241 tggggatgcc gcagatgagg acgcgcaggg gtttatctat gccttggcgg tggggaatca
     3301 tggcacgaca ggcttgggta attttgccgt gtgcgcctgt ttcggaggaa tcgagggcga
     3361 tggcgcaggt gtcggggcgg ctgttatagt gttcgagcca gattttggtg cgctcggggt
     3421 cggcaagatc ttgtttgttg aggattttaa gtttgggttt acctttggaa agctgggcaa
     3481 gcagggggtt ttcgctggag gcgggcatac gcgcgtccag catttcaatc accatatcaa
     3541 cgctttttgc acgctcggcg atggcttttt tcgccttgtt catatggccg ggaaaccatt
     3601 ggattgccat gtctgttctt tcttttcaat atttgaaatg ccgtctgaaa cggaggacgg
     3661 ggtttcagac ggcataatgg tttgacggaa ttagcggtct gacaggtttt gcgcctgtct
     3721 gtgccgtatc agcaattcat aacgcccttt caaccttcgg gcgagttttt cgacaatata
     3781 gaccgaacgg tgctgtcctc ccgtgcaacc gatggcgacg gtaacgtagc tcctgctttc
     3841 atcctccaaa cgcggtaacc aatgcgtaac aaacctttcg atgtcgtcaa ccatttcctg
     3901 cacaagcggc tgtccgtcca aataatccca aacgggcttg tccataccgg tgtaaggcct
     3961 caactcggga tcgtaatacg ggttgggcag gctgcgcata tcgaacataa aatccgcgtt
     4021 gttcggcaca ccgtatttga acccgaagga ctccaaaatc accagcagcc cggtacgttc
     4081 gaccttcagc cactgccgga ctgcatggcg gagctgttgg gcattcatct tggaagtgtc
     4141 gatacaatag gcgatttctt taagcgggaa cagccattcc cgttctttct ttaagctttc
     4201 caacaaggtc atatcctgat tgctcagagg atgtcctcgc ctggtttcgg aaaaccggcg
     4261 gaccaacacg ctttcttccg cctcgacaaa caaaacttca accctgtgcc ccagtccgcg
     4321 cagagaggca atctgttccc gcgcctgtcc gatgtcaatg ccggaacgca catcgacgct
     4381 gaccgccaat tcggtttcgt ccgcacgttc gatatgatac gacaccagcg cgggcaacat
     4441 ttccaaaggc aaattgtcca cgcagaaata acccgaatct tccatttggc gcagtgcgac
     4501 ggacttgccc gaaccggaca ggccgctaat caggacgatc ttcattgtgt tgttcgtttt
     4561 ctttaagttg cgtctgatgg cgttccaaaa attcgcgcgt actgtcctta ccgcgcaact
     4621 gcaaaatgta attgcgtacc gccgcctcaa ccaaaacggc gaggttgcgt ccgacggcga
     4681 cgggcagcgt aaccgaacgg acgttgacgt tgaggatgga ttcggtttcg gtgcggatgc
     4741 tcaaccggtc aagctgcttc atatactcgt cgtccgcctc gactaaattg ataatgagtt
     4801 gcaggatttt tttggggcgg atggaagttt cgccgaaaat atggcggata ttgagtatcc
     4861 ccaagccgcg cacttccaaa aaatcgcgca gcataggcga acaacgccct tccagcgttt
     4921 ccgggccgat gcggaacagc tcgaccgcat cgtcggcaat caggctgtgg ccgcgcgaaa
     4981 tcagttccaa tgccaattcg ctcttaccca ggccggaatg cccggtaatc agcacgccga
     5041 tttcaaacac atcgagaaat acgccgtgtt tgacggacga tgccgccaag gtgcgttgca
     5101 ggtaaatccg caacacgtcc atcagatagg ggctttcgag tttggaagtc agcagtggaa
     5161 tatcgttttt atgacaatag tcgcgcagtc ccggggaaac cggcaagccg tttgccacaa
     5221 taaccaaaga catagaaata tcgaacaggt cgccaaactg ataacccgtt tcccccgatt
     5281 cgaggcggtt cagatattcc gactctgcca aaccgaccac ttggatttgg ttgggatgaa
     5341 tgaaattcag gtgtccgact agggcgagga cgggcttgtc cgcctctacg ccgatacggt
     5401 tgtccgcacc cgaattgccg gcggcccaag cgagttgcag tttgtattgg ttgtcatcaa
     5461 acaggcggcg gacggagata ctgggcatat tattcttcag tcaggatggc acggacttct
     5521 tccgcagagg aaaccgtcat cagcgattct ctgatgcttt tttgggaaaa cttgccggcc
     5581 agtttggata agacttccaa atgctcgccg gttgcgtttt ccggaaccag caagataaaa
     5641 atcagggaaa ccggcttgcc gtccggtgcg tcaaatccga cgggttcgcg cgtgcggatg
     5701 aacgcgcccg tcgcctgctt cacgccggca tgacggccgt gcgggatggc aacgccctgc
     5761 cccaaaccgg tcgaaccgag tttttcacgg gcaaaaagac attcgaaaac atcagcatgg
     5821 gacaatgagg attcgcgttc caaaagcagg cctgcttcct caaacagcct ttttttactg
     5881 cctacctcca tatccaaaac aatatgggac aaaggcaaaa tttcgccgat aaggctcata
     5941 agcttctctt ttcagacatc gcaaaacaga aagattgtac cgactgccgg ggcaaatctc
     6001 aatcccgcat acggtacggg ctgacataac acagcgtttt aaaaacatat tttaacgctt
     6061 ttcggcacag atagaaatgc cgtccaaagc agtttacggc tcttcagacg gcattgccct
     6121 gccttatttc gcgaaaaacg tatgggcaaa aatggcggta aaccacaccc aaccaattct
     6181 gttgtttgcc aaaaacgttt caaaacagat ttgccggacg cggcttttga tggcggcata
     6241 ttggcggtat tgcagcagca ggacgatggg gattgccgtc caatatgccc atgccgcacc
     6301 gataaccgca cccaatactg ccatcagcag ggtaaagcct ccgtgacaca gcataacggc
     6361 ggcgatgtcg taacgcccga acgtgacggc ggaggttttg atgccgattt tcaaatcgtc
     6421 ttctttgtcc gccattgcat aaaccgtgtc atacgccaga gtccataaca cattggcggc
     6481 aaagagtatc cacgcttgag gcggcacgtt tccggcaacg gcggcaaacg ccatcgggat
     6541 accgaaggaa aaggcaagcc cgagatagag ttggggaatc ggaaaaaaac gtttggtaaa
     6601 cgggtaagtc agcgcaagaa acagcgcggg caggctcatc agccaagtca gatgattcag
     6661 cggaatcagg cacaatgcgg caagcaggca caaaaatgcc gtcagcagca gcgcttcttt
     6721 tttcttgacc ctgccctgtg cgaacggacg gttttttgta cgctcgacag caccgtcaaa
     6781 atcgcggtcg gcaaagtcgt tgatgacgca gccggcactg cgcattaaaa acgtgccgat
     6841 tgtaaacgcc gccaataccg ccaaatcggg aatgccgtct gaagccagcc acaatgccca
     6901 gtaggtcggc cacagtaaaa gcagcgtccc aatgggcttg tccgcccgca tcaggcgcag
     6961 gtacacatcc aaacggtcgg acaggcgtaa aaataaaggg gatttaggat tcatattgcc
     7021 gcgcagcttg aaaaaacggt attttatccg ataaaacgtt tcagttcggg cagaaaatac
     7081 tcggtcagca gcatttcctc gccgtgacgg gaaaaccgag aacgccgcgc ggcaaagtac
     7141 cgtccgcatc cttcgccggc aacggcaaac tcaaacgccg aacgcgcccc ttccaaatcg
     7201 gcttgaaaca gacgctcgcc caaaggacgc gtgccgcagt ccaaaatgtt ttgccaaaac
     7261 gccgaaccga tacggcattc gctccttgcc tcaacaacag ggatacggtc cagcttcaac
     7321 aaaacttcgc gcaccagcct ccctccgcat tccgtctcca attcgcccag tttcagcagt
     7381 tccaccgaaa atgtatgcgg caaggcgcgc aatgcggcgg tcagcgaccg ggtgtgcagc
     7441 agccgcacca tcggcaggct gatgccgtct gaaatggcgg cgggcaagtc gggcagccat
     7501 ttcccaaata ggtgttccat atttttccca atctttatac cgcgtctgtt tttgccaact
     7561 ccatccattc cgcttcggaa aaatcggctt tcagacggca tttcaagtag ctcaggctgt
     7621 cttgggcgat gtgtgcgtcg cggctgtcgt ggatgtggtt gaacacgagg ctgtgcaagc
     7681 gaatgccgta ttgtttgagc gcggcgaaac tgagtaaagt gtggttgata ctgccgagcc
     7741 gtccgctggt aacgaggatg acgggatagc cttgctgacg gatataatca atggttaaca
     7801 ggttttccgt cagcggaacc atcaatccgc ccgcgccttc gaccaaaacg acttcgtact
     7861 gcgccgccaa ttcttgtgtg gcggtgcgga ttttgtccaa gtccaaagcc ctgccatcca
     7921 gtcgggcggc gaggtgaggc gaagcgggat agctgaagat ttcgggcata gtcagccgcc
     7981 gtttgtcggc ttcctgcatc ggtatgccca taattttgcg gtggacggcg atgtcgtcgg
     8041 caatgttttg gcaaccggtt tgcacgggct tttgcgtaat cacgcttttg ccctgctgca
     8101 acaattgttt tgccaacacg ccggtggcga cggttttgcc gatgtccgtg tctatgccgc
     8161 tgacgaagta aacgcctttc atttgctgtg ttccttcaag atttgcacgg ttttgtcggc
     8221 aagtttggtc aaaacgccgt ctgaaatgat atagggcggc atcagataca ccagcctgcc
     8281 gaacgggcgc acccaaatgc cctgcgccac gcagtccgct tgaaaacgcg ccatatccac
     8341 gcctttttcc agctcgatca ccccgatggc acctaaaacg cgcacgtctt tcacgccgcg
     8401 aatgtcccac gcggctttca gacggccttt taagatgctt tcaatgcggc ggatatttgc
     8461 ctgccagtct tgagacaaaa gcagtttgac cgaagcgcag gcaacggcac acgccagcgg
     8521 gtttgccata aacgtcgggc cgtgcataaa cacgcccgct tcgccgcgcg aaatcgtttc
     8581 ggtaactttt tgcgaagtga ttgctgccgc cagcgtcata tagccgccgc tcaaaccctt
     8641 gccaatacac ataatatccg gcacgacctc cgcgtgttcg caggcaaaca tcttgcccgt
     8701 gcgcccgaat ccagtggcga tttcgtcaaa aatcagcatg atatcaaatt cgtcgcacaa
     8761 atcgtgcaat ccgcgaagat actgcggatg ataaaaatac atgccgcccg cgccctgcac
     8821 gaccggctct aaaataaagg cggcaatatc cgcatgatgc acttcaaata aggcgcggac
     8881 aggctgcaaa tccgccccgt cccattcatc gtcgaaacgg cttttcggat tatcgacaaa
     8941 ataacgctgc ggcaacgcgc tgccgaaaat atggtgcatc cccgtttccg gatcgcagac
     9001 ggacatcgcg ttccaagtat cgccgtgata cccgcggcgc accgtcgcga tattctgctt
     9061 cgccgtcaaa ccccgcgcct gctggtattg cactgccatc ttcagcgcaa cttccaccga
     9121 aatcgaaccc gaatccgcat aaaaaatacg gttcagcccc tgcggcaaaa tcccgaccaa
     9181 caacttgccc agctccaccg ctggctcgtg cgtcaaacca ccgaacatca cgtgcgccat
     9241 ttgtttcatc tgcgtctcaa ccgcctgatt caaaacagga tgattgtagc cgtgtatcgc
     9301 acaccaccag gaggacatcc cgtcaatcag ccgcgtgccg tccgccaatt cgataaacac
     9361 cccttctgca cgtttgacag gataaacggg cagcggatcg gtcatggaag tatagggatg
     9421 aagcagatgg gtacggtcga aatcaagcaa tgatgatatg tgttgatgtt cagacggcat
     9481 aagtttctct cttttcttct tactgtattc aaacgcaaaa cgcgtattct actccgacag
     9541 accgtttccc acacctctcc atccgtttcg ggcgcaaaac cgcgaaacaa atcgtccgca
     9601 gtataagcgc acaccgtttc gcattcccca agcccgattg gaatcagacg gcccaacgcc
     9661 caataccgtt tcacgcagcc tccgcccaac gcttcaatca gtcatagata tagtggatta
     9721 acaaaaatca ggacaaggca acgaagccgc agacagtaca aatagtacgg caaggcgagg
     9781 taacgccgta ctggtttaaa tttaatccac tataaaacgg caatccatac gatacagatc
     9841 atagcaacag ccatcgcaac agcgttagca aaatcagggg actccgacat aggcgcatag
     9901 cacctaccga tgcacggctc ctcattcggc tctatgaata ccatacccat cacaaaatcc
     9961 accgccaaaa ccaggcacgg cttcttatac ttatgataga tttccaccat cctgtcccat
    10021 atataccaaa cattcatacc gtatatcccg caggcaacaa attccgattg aaggttacag
    10081 ccctatt
//
LOCUS       DAWGRZ010000059         9887 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000059 DAWGRZ010000000
VERSION     DAWGRZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9887
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.059"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(246..974)
                     /gene="rmpM"
                     /locus_tag="WHT44_001059"
     CDS             complement(246..974)
                     /gene="rmpM"
                     /locus_tag="WHT44_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ2533252.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1338..2288
                     /locus_tag="WHT44_001060"
     CDS             1338..2288
                     /locus_tag="WHT44_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ2533253.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2401..3135)
                     /gene="fnr"
                     /locus_tag="WHT44_001061"
     CDS             complement(2401..3135)
                     /gene="fnr"
                     /locus_tag="WHT44_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ2533254.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3340..4761
                     /gene="hemN"
                     /locus_tag="WHT44_001062"
     CDS             3340..4761
                     /gene="hemN"
                     /locus_tag="WHT44_001062"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ2533255.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(5114..6688)
                     /locus_tag="WHT44_001063"
     CDS             complement(5114..6688)
                     /locus_tag="WHT44_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ2533256.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            6718..6911
                     /locus_tag="WHT44_001064"
                     /pseudo
     CDS             6718..6911
                     /locus_tag="WHT44_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7009..8109
                     /locus_tag="WHT44_001065"
     CDS             7009..8109
                     /locus_tag="WHT44_001065"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ2533257.1"
                     /translation="METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGR
                     LRRQLLDLQDTGADELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAERTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDNRE
                     TRAVLNIGGIANISVLPPDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVC
                     DAVSHAAADARQMYICGGGIRNPVLMADLAECFGTRVSLHSTADLNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            8229..9170
                     /gene="mafA"
                     /locus_tag="WHT44_001066"
     CDS             8229..9170
                     /gene="mafA"
                     /locus_tag="WHT44_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ2533258.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTKNSGRKGERSAGLSVNGTGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLITPKTAAYESQYQEQYALWTGPYKVSKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKKPDVGNEVIRRRKGG"
     gene            9174..>9887
                     /gene="mafB"
                     /locus_tag="WHT44_001067"
     CDS             9174..>9887
                     /gene="mafB"
                     /locus_tag="WHT44_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ2533259.1"
                     /translation="MKPLRRLTNLLAACAVAAAALIQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASEEKGNVDEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKL
                     DRWGNSMEFINGVAAGALNP"
BASE COUNT         2520 a         2936 c         2370 g         2061 t
ORIGIN      
        1 gcggcaatgg gacacaaacc tgaatctgaa tctgaatctg aatctgaatc tgaatctgaa
       61 tccgaatccg aacctgcacc ccgattctga cgatagccgt ttcagaaaac cacccaaaaa
      121 aacaaacccc gcaaaagcgg gggtttgttt cacatcatcg atgccgaccg acgtttcacg
      181 aatattgcaa cagtacctgg tacaaaatcc actaacctca tgcatcggca agatattgcc
      241 tagccttagt gttggtgatg attgtgtgcc ggcacaacct gacgggttac gatgctgcgg
      301 attttcacat ccacacggcg gtcaggttcg atacatgcaa tcagagcctc acgttttttg
      361 gctttagaga ctttcgcacc cagtttggca acttcggctt cacaaacttg agtcatttgc
      421 gcttgagatt cgcccaagcc gacagcagaa attctagaaa caggtacgcc gttgctgacc
      481 aggttgtttg ccactacgta tgcgcggcgt tcggacaggg cctgattgta tttgtcagaa
      541 cccataaagt cggtatggcc ttcgacgcgg acagattgga cattggttcg actcaggcgt
      601 tgcgccaata ctttcaggtt gtcttgagct tcggcgcgca atgaatcctt atcgaaaccg
      661 aacagggttt tggcagacag ggaaatggtt tcatcaacat attgcggagc ctgctccaca
      721 acgacgacag gcgcgggtgc gggttcgggt tctggctcgg gttcgggggc agcaaccgca
      781 tcgccgcatt ctacgcgacc ttggcttgct ttatcaaagt aggcgttttt ccagcattcg
      841 ccatagttgt tgcgtacgat ttcgttcgac tggccgctta cggtgtaacc ctgaacggac
      901 gcctcgcccg caacagcagt gccggaagcg agcaatgcaa cgaataatgc gcttaatttc
      961 agctgtttgg tcattttatt ccctcattaa atttgtacag cagatacaga agcaactgct
     1021 gaattgccaa catacaggcc gctgtagaaa catggcggca aatcggatat ttcagtctcc
     1081 actataaaga agcagggggc ggactgtcaa tactcaacgc ctgaaaacaa ccgtaaaaca
     1141 gcctgtttcc tgatagtcta tcatgccgaa aaatcaattc cgtgtcatta ctttaggggg
     1201 attttttcca tatcgcacga ttgccgttgc acaaaaacaa caaacacaac ggcaaagccc
     1261 cataccgtac cggcaagaaa atatgataaa ttataaacaa tgttcagcca cccgacacag
     1321 acccacaccg acccgccatg aaattacaac aattgaaata cgccttagaa gtttaccagc
     1381 acaacctgaa cgtttccgaa gcggccgaag ccttgtttac ttcgcaaccc ggcatctcca
     1441 aacaaatcaa attgctggaa gaagaaatcg gcattcagat ttttatccgc agcggcaagc
     1501 gcgtggtttc ggtctcgcag ccgggcaagg tggttttgga tattgcggaa cgtattttgc
     1561 gcgatgttca gaacattaaa aatatcggca gcgagtttac cggacaggac agcggttcgc
     1621 tgacggttgc cacgacgcat actcaggcac gctatgccct acccttgatt gttgccgatt
     1681 ttgtgaaacg ctatccgaaa gtcaacctga ccatcaaaca ggggagccct gccgccatcg
     1741 cacgtatggt tacctcaggc gaggcggact tggcgattgt tacggaacgg atagacgacc
     1801 atcccgaact gggaaaactt ccctgctatg actggactca tgcggttatc gtaccgaacg
     1861 accacccctt gctcgaatgc agaaaccccc tccgtattga agatttggcg aggtttccgc
     1921 tgattactta tgaatttgca ttcaatgcgg gcagcagcat cgcgcgggca ttttccaaag
     1981 cccgtttgga acaacccgat gtcgcattgg ctgcggcaga tacggacgta ttgaagactt
     2041 atgtgcgctt gggtttgggc gtgggactga tggcgaaaat ggcgtacaac ccggatacgg
     2101 acggcgattt gcagcttgtg gatgcggcac acctgttcga gccgtcgccg acgtggattg
     2161 ctttgcgcag cgatacttat ttgcgcggat atgcctacga ctttatccaa gcgttcgcgc
     2221 cgcacctgac acgcgagaag gtggatagga ttctgtacac gcccatcagc gaggattttt
     2281 cgatttaggc ggctgccggt tttcagacgg cactttgcgg cagatacaac aaacaggaca
     2341 gatgttttcg tctgccctgt gtttattgag aatgctgtct gaaatgttcg tacgggttaa
     2401 tcaaatggcg tgcgagcagc cggacaccat ttttttcaac acctgcagat tgaggatttt
     2461 gatgtgctta tgctcgacgg aaatcaatcc ttcctgatga aatttagata atgtgcggct
     2521 gacggtttca agtttcagcc cgagataact gccgatttct tcgcgggaca ttcttaagat
     2581 gaagtcgttg gcagcaaaac ctcgggaata aaggcgttgg gaaaggttca gcaggaaggc
     2641 ggcaatccgc tcttcggcgc gcatattgcc caacagcagc ataacacctt ggtcgcgcac
     2701 gatttcacgg ctcatcatgc ggaagaagtg cgtacgcagg ctggggatgt tttgccccag
     2761 ttcttcgatg tgggtaaacg gcagttcgca cacttcgctg tcttccaagg cgaccgcgtc
     2821 gcaactgtgc acatgggaac agatgccgtc catgccgatg agttcgcccg acataaagaa
     2881 acccgttacc tgatcgcggc cgtcctgact ggcgacggtt gttttgaaga agcccgaacg
     2941 gatggcaaag agcgaggtaa aggcttcgcc gacacagaac aggtattcgc cctttttcag
     3001 gcggcggctt tgacggatga cggcatcgag ttggctgagc tcgttgggca gcagcccgac
     3061 aggcaggcag agttcccgca aagaacagga agaacacagc gttttcatct gatgtgtagt
     3121 attatgcgaa gccataccgt acctttttgt gcgctttgcc ccatcatgat tatagtggat
     3181 taaatttaaa ccagtacggc gttgcctcgc cttgtcctta tttaaattta atccactata
     3241 tgtgcttatt gacacatatc aagacaggtt tatcatactg tggcattcta ccaaactatc
     3301 caaaatttga ctaacatcat aaaaccgcac acacacacca tgaaaatcat tcagatacag
     3361 aacaatcaca atgtaaacga tgaccgcccc gagtttgacc gcgcgctgat tgccagcctg
     3421 cccgccagcg gcccgcgcta cacttcctac cctaccgccg accgtttcca tgacggtttc
     3481 cgcgaaggcg aatatatcaa agctttacat ttgcgcggta tgggcgcgtt aaacaaaccg
     3541 ctttcccttt acattcacat tccgttctgc aacaccatct gctactactg cggctgcaac
     3601 aaaatcatca ccaaagacaa aagccgcgcc gatgcctaca tcgaatatct tgaaaaagaa
     3661 atggaactgc tcgctccaca tctgaacgga cggcaccagc ttgcccaact gcacttcggc
     3721 ggcggcacgc cgaccttttt gagcgacgaa cagatcgaac gtgtcttccg catgatacgc
     3781 aaacatttcg agttaatccc caccggcgaa tactccatcg aaatcgaccc gcgcaaagtc
     3841 agccgcgaca ccgtcctcat gctcggcaga ctcggcttca accgcatgag catcggcatt
     3901 caggatttcg accccaaagt gcaggcggcg gtcaaccgca tccaaagtta cgaagaaacc
     3961 aaagaagtca tcgatgcggc gcgcgaagcg gggttcaaat ccgtcagcgt cgatttgatt
     4021 tacggcctgc cgcaccagac ttcggaaagc atcaaaacca ccatcgatac cgttttgtcg
     4081 ctcgatcccg accgcctcgc cctttatcac tacgcccacc tgccgcacgt gttcaaaccg
     4141 caacgccgca tcgataccgc cgccgttccc gacagcgaag agaagctcga tatgctgcaa
     4201 tactgcgtcc aaaccctaac cgaacgcggc tacgtcttca tcggcatgga tcatttcgcc
     4261 aaacctgacg acgaactctc catcgccctc aaagaaggct tcctccagcg caacttccaa
     4321 ggctattcga cctacgcgga ttgcgatttg gtcgccatcg gcgtgtcgtc catcggcaaa
     4381 atcggcagca cctattccca aaacgaacgc gacatcgatg cctactatgc cgccatcgac
     4441 gaaggcagac tgcccatcat gcgcggctac cagctcaatc aggacgacat cctgcgccgc
     4501 aacatcattc aggatttgat gtgccgtttc gcgctcgact atcggattta cgaaagtatg
     4561 ttcggcatcc cgttcgaccg ctacttcaaa gacgaactgg cggatttgga aaaactcgcc
     4621 ggtttgggat tggtgcgcct gaacagccac gggctgaccg ttaccccgaa aggacgcttc
     4681 ctcatccgca acatcgccat ggtcttcgat taccacctgc gccataaaga aaccaaggcg
     4741 aaatattcgc aaacagtgtg attgtggcta acgtacaaat gccgtctgaa aggctttttc
     4801 agacggcatt ttgctgccgg caggataagt gttttcaaga acaggcggcg gcatatcata
     4861 acgttccgca cctttgtgtc cgaccgttcc gaaaccaaga tatagtggat taacaaaaac
     4921 cagtacagcg ttgcctcgcc ttgccgtact agctgtactg tctgcggctt cgtcgccttg
     4981 tcctgatttt tgttaatcac tataccaaac ggcatatccc gacagaacag attgtgcata
     5041 aggcacaagc ccgcacattc catcaacaaa atgccgtctg aacacgggtt cagacggcat
     5101 cagtatttta caatcagaat actgcctgta aaaccaagta gcagacaacc gacaatacgg
     5161 cggcggcagg cagggtaatg acccacgcca aaccgatggg cttcatcagt ttccagttgg
     5221 cattgcggtt gaccagaccg ataccgagta ccgcgccgac caagatatgc gtactggaca
     5281 cgggcagccc catcagcgac gcgcccatca cgacggaggc ggcggacagt tcggcggtaa
     5341 aacccgaagc aggatgcatt tccgccaaac tcgtaccgac ggttttaatc acctctttac
     5401 cgacaaacca caaaccgaca atcagcgcga tgccgaaagt cagcatcgca atcgggggga
     5461 cgacattttg cgcggcaacg ctgttggtac gcaaaacatc cataatcgcg gcaaacggac
     5521 cgatggcgtt ggcgatatcg ttcgcaccgt ggctgaatgc gaagccgcag gcggtaaaga
     5581 cctgcatcca tgaaaacatc tgaaaggtcg atttgcccaa gtctttacgc ttgaggcttt
     5641 tggcaaaaac aaacgtcccc atccacaccg ccgcgcctat cataaagatg gtcaggaagc
     5701 tgttgacgtt gctcatcccc aaatgcaggt ttttcaagcc cttgaaaatc agcatagcgg
     5761 aaatcatcat cgcgccgaac gaagcgataa agggaatcca agaatgcagt gccttgtagg
     5821 aatcgacatt gtttttacgg ttgtcgaacg cataaagacc gcggtaatac tccgattgca
     5881 gctcttgcgg atcgaattcg ggttcgtcgt aaatttgcgc gtcgtgcgcc attttggtgg
     5941 cgtactcgac tttttcggct tcggacaaac cctcgaaaaa caggcggtgc cgttctttat
     6001 aggccttttt ttcctgcttg atgcccttga gcgtgccttc cgcccaagcg ttgtaatcta
     6061 agacgttttt cttgacgcgc gaaaacagaa aataggacac cgcgccgccc aacacgggcg
     6121 acaataccca agaaacacca ataccgccca gcttgcccca acgtatcaaa tcgcccgatg
     6181 cggcatcgtt cattaccgcc atacataccg cgctgccgac aatgccgccg ataatggaat
     6241 gggtggtaga taccggaagc ccttttttcg aggcaaacaa cagccacaac gccgccgcca
     6301 aaagcgcgga catcataata aacacaaact gtatgggttc gaaatcaaca cccttcaaat
     6361 cgacgatgcc tttgcgtatg gtattggtta cctcgccgcc cgcgatgacc gcgccgctga
     6421 cctcaaatac cgccgcaatc agcaaagcct gcgggatggt cagcgtaccc gcaccgacgc
     6481 tggtgccgaa agaattggca acatcgttgc cgccgatgtt gaacgccata aacacgccga
     6541 acatcgtggc aataatgaat aaggcggcgt aattgtaatg ggtataaccc aagccccaat
     6601 aaataaaata gccgactgta cctaccagca tggcggcaaa gacggcgttg atggttttaa
     6661 catttgcgct catttggatt tgagccatgg caatcttcct ttaatgtggt ggaaatcatg
     6721 agtgttgccg acactgtact gctgcctccg gtcgaactgc cgtttcggga tatggcagac
     6781 gcatccttcc taaccgcatc cgaatacccc caaacctgac cgaatgcaaa actgctgccg
     6841 gaaacggttt gtatgacact tctactactg gatgttgcgg gcgcattata atatttttcc
     6901 atccgtcttg aaacatttat ttacacttta tttacactgc ggcggcaaat cggtatacga
     6961 gcgtcaatac acgttaaaat ggcgttttgc accagtttgg gagtgatgat ggaaacacag
     7021 ctttacatcg gcatcatgtc gggaaccagc atggacgggg cggatgccgt actgatacgg
     7081 atggacggcg gcaaatggct gggcgcggaa gggcacgcct ttacccccta ccccggcagg
     7141 ttacgccgcc aattgctgga tttgcaggac acaggcgcag acgaactgca ccgcagcagg
     7201 attttgtcgc aagaactcag ccgcctatat gcgcaaaccg ccgccgaact gctgtgcagt
     7261 caaaacctcg caccgtccga cattaccgcc ctcggctgcc acgggcaaac cgtccgacac
     7321 gcgccggaac acggttacag catacagctt gccgatttgc cgctgctggc ggaacggacg
     7381 cggattttta ccgtcggcga cttccgcagc cgcgaccttg cggccggcgg acaaggcgcg
     7441 ccactcgtcc ccgcctttca cgaagccctg ttccgcgaca acagggaaac acgcgcggta
     7501 ctgaacatcg gcgggattgc caacatcagc gtactccccc ccgacgcacc cgccttcggc
     7561 ttcgacacag ggccgggcaa tatgctgatg gacgcgtgga cgcaggcaca ctggcagctt
     7621 ccttacgaca aaaacggtgc aaaggcggca caaggcaaca tattgccgca actgctcgac
     7681 aggctgctcg cccacccgta tttcgcacaa ccccacccta aaagcacggg gcgcgaactg
     7741 tttgccctaa attggctcga aacctacctt gacggcggcg aaaaccgata cgacgtattg
     7801 cggacgcttt cccgttttac cgcgcaaacc gtttgcgacg ccgtctcaca cgcagcggca
     7861 gatgcccgtc aaatgtacat ttgcggcggc ggcatccgca atcctgtttt aatggcggat
     7921 ttggcagaat gtttcggcac acgcgtttcc ctgcacagca ccgccgacct gaacctcgat
     7981 ccgcaatggg tggaagccgc cgcatttgcg tggttggcgg cgtgttggat taatcgcatt
     8041 cccggtagtc cgcacaaagc aaccggcgca tccaaaccgt gtattctggg cgcgggatat
     8101 tattattgag cgcaaatgcc gtctgaaagc catttccgcc gctcagacgg cattttcgcc
     8161 ccttttgctt acaaaccctt aaaatccctt tacactcaaa atccgttcaa catcaaacaa
     8221 accccgctat gaaaaccctg ctcctcctca tccccctcgt cctcacagcc tgcggcacac
     8281 tgaccggcat acccgcccac ggcggcggca aacgctttgc cgtcgaacaa gaactcgtcg
     8341 ccgcatcgtc ccgcgccgcc gtcaaagaaa tggatttgtc cgccctaaaa ggacgcaaag
     8401 ccgcccttta cgtctccgtt atgggcgacc aaggttcggg caacataagc ggcggacgct
     8461 actctatcga cgcactgata cgcggcggct accacaacaa ccccgaaagt gccacccaat
     8521 acagctaccc cgcctacgac actaccgcca ccaccaaatc cgacgcgctc tccagcgtaa
     8581 ccacttccac atcgcttttg aacgcccccg ccgccgccct gacgaaaaac agcggacgca
     8641 aaggcgaacg ctccgccgga ctgtccgtca acggcacggg cgactaccgc aacgaaaccc
     8701 tgctcgccaa cccccgcgac gtttccttcc tgaccaacct catccaaacc gtcttctacc
     8761 tgcgcggcat cgaagtcgta ccgcccgaat acgccgacac cgacgtattc gtaaccgtcg
     8821 acgtattcgg caccgtccgc agccgtaccg aactgcacct ctacaacgcc gaaaccctta
     8881 aagcccaaac caagctcgaa tatttcgccg ttgaccgcga cagccggaaa ctgctgatta
     8941 cccctaaaac cgccgcctac gaatcccaat accaagaaca atacgccctt tggaccggcc
     9001 cttacaaagt cagcaaaacc gttaaagcct cagaccgcct gatggtcgat ttctccgaca
     9061 ttacccccta cggcgacaca accgcccaaa accgtcccga cttcaaacaa aacaacggta
     9121 aaaaacccga tgtcggcaac gaagtcatcc gccgccgcaa aggaggataa accgtgaaac
     9181 cgctgcgcag actgacaaac ctccttgccg cctgcgccgt agcggcggcc gcactcatac
     9241 agcccgccct cgcggcggac ttggcgcaag acccgttcat taccgataac gcccaacggc
     9301 agcactacga acccggcggc aaataccacc tcttcggcga cccgcgcggc agcgtttccg
     9361 accgcaccgg caaaatcaac gtcatccaag actataccca ccagatgggc aacctgctca
     9421 tccaacaggc aaacatcaac ggcacaatcg gctaccacac ccgcttttcc ggacacggac
     9481 acgaagaaca cgcccccttc gacaaccacg ccgccgacag cgcgagcgaa gaaaaaggca
     9541 acgttgacga aggctttacc gtataccggc tcaactggga aggacacgaa catcatcccg
     9601 ccgatgccta cgacggcccg aagggcggca attaccccaa acctacgggc gcacgagacg
     9661 aatacaccta tcacgtcaac ggcacagccc gcagtatcaa actcaatccg accgacaccc
     9721 gcagcatccg gcaacgcata tccgacaact acaacaacct cggcagcaat ttctccgacc
     9781 gcgccgatga agccaacaga aaaatgttcg agcacaatgc caagctcgac cgctggggca
     9841 acagcatgga gtttatcaac ggcgtcgccg ccggcgcgct caacccc
//
LOCUS       DAWGRZ010000060         9845 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000060 DAWGRZ010000000
VERSION     DAWGRZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9845
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.060"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(62..676)
                     /locus_tag="WHT44_001068"
     CDS             complement(62..676)
                     /locus_tag="WHT44_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ2533260.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIEDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
     gene            complement(713..2863)
                     /gene="dinG"
                     /locus_tag="WHT44_001069"
     CDS             complement(713..2863)
                     /gene="dinG"
                     /locus_tag="WHT44_001069"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ2533261.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYRLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSLGVSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVIGLNDALS
                     AARREKEQDGALLDRLTSEFGLFIARIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            2966..3190
                     /locus_tag="WHT44_001070"
     CDS             2966..3190
                     /locus_tag="WHT44_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533262.1"
                     /translation="MVFWLQRLKYPLLLWIADMLLYRLLGGAEIECGRCPVPPMTDWQ
                     HFLPAMGTVSAWVAVIWAYLMIESEKNGRY"
     gene            complement(3258..4559)
                     /locus_tag="WHT44_001071"
     CDS             complement(3258..4559)
                     /locus_tag="WHT44_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ2533263.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCAAVAEGLPI
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRTV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAYADFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPQPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAAAHKTDPDGT
                     FVRRHIPELAHLSADTVHTPWRFACSVDTHGYPAHPVAGIP"
     gene            complement(4549..5460)
                     /locus_tag="WHT44_001072"
     CDS             complement(4549..5460)
                     /locus_tag="WHT44_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ2533264.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            5568..5966
                     /locus_tag="WHT44_001073"
     CDS             5568..5966
                     /locus_tag="WHT44_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ2533265.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            6015..6620
                     /locus_tag="WHT44_001074"
     CDS             6015..6620
                     /locus_tag="WHT44_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ2533266.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVVGGIEGAAGEKTFEPVAERLKVFGA"
     gene            6806..8916
                     /locus_tag="WHT44_001075"
                     /pseudo
     CDS             6806..8916
                     /locus_tag="WHT44_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            9031..9726
                     /locus_tag="WHT44_001076"
     CDS             9031..9726
                     /locus_tag="WHT44_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ2533267.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
BASE COUNT         2128 a         2525 c         2893 g         2299 t
ORIGIN      
        1 cgttttgagg ccggggtcgg aataaccgtt ttttgatgat tttccctccc cggctgtgtc
       61 atcaaaaccc caattgcctt tccaaactct ccaccagatt gtcatccagt ttcaaagcct
      121 gcgacaggcg ggcgaggaag acggtttctt tccgcgacaa atcggcacag accaaccttg
      181 ccgccagata ggcctccgcc gccaacgcct catcgttgcc gacggcggcg gcgatgtctt
      241 cgatgcttgc gggaaggcgg tattcggcgg cgagccatgc ggcagtttcg gggtctgtgc
      301 cgctttcctg ttcgatagtc cggcgttcgg cttcgtctat catgccgtct gaagcggcgg
      361 cggctatcat ggtgcgcaat acggtacggc tgtatgtttc ttcagtttct ccggcaggtt
      421 ggaaatcgct ttgtgttacg gttgcccgcc ctttgttttg ctgccacatc tgatagcccc
      481 ggtaggcgag gtagcccaaa gcggcggtcg aaccgatttt ggtgatggtt ttgcggtttt
      541 taccgttcag cagcatggag gcgacaccgg caaccagcgc gcctccgccg aatgaattga
      601 gcgggctgtc ggagaatgtg ttgccttttt tttgaaccgt gcttaagact tggttgagca
      661 gtcgggtaaa gttcatgaat ttttcctttc tgttgcggga agggcggtat gtttacccta
      721 tccttttaaa cggcggcagg ccggccaata attgttggcc gtaccgctgt gttttgatgc
      781 ggttgtcgag gatggttacg cggccgtagt cttgttcggt gcggatgagg cggccgacgg
      841 cctggatgag tttgatgccg gcttcgggga cggtgatttc gatgaagggg ttgccgccgc
      901 gctgttctat ccagcggttt tgggtttttt cgatggggtt gtcgggcatg gcgaagggaa
      961 gtttggcgat gatgacttgc acgcaggcgg tgccgggcag gtcgagtcct tcggcaaagc
     1021 tgtcgagtcc gaagatgatg ctggctttgc cttcttctat ggcccggtgg tgtttttgca
     1081 ggaggacggc tttgggtaat tcgccttgta cgagcaagag cggcaggtag tctccgggca
     1141 ggcgcagggc gacatcctgc atttgtttgc gcgaggaaaa caagacgagc gtgccgatgg
     1201 cttcggtggg cgaaataagc ttgggcagcc attcgatgac ggcggcggtg tgggcttcgg
     1261 ggtctttggg gctggcgtat atggggggga tgtagagttc gccctgtttt tcaaagtcaa
     1321 aggggctttt gagggcgagg gtggtggttt cgggcagcca ttgcagcccg gtttggcgca
     1381 gcatcaggtt gaagttgccc aaggattgca gggtggcgga agtcaatacc gcgcctgccg
     1441 cacgccgcca caggctgttg gcaaggtggg atgcgctgct gatggggctg gcgttgaaaa
     1501 tgtagtcgtt tttgtcgtcg gcgcggcggg ttatccattt cgccaacggt tcttcaccct
     1561 cgagggggac agtggagagc aaatcccaaa ccgcgctgat ttgttcgata cgggcgataa
     1621 aaagaccgaa ctcgctggtc aggcggtcga ggagcgcgcc gtcctgttct ttttcgcggc
     1681 gtgcggcaga aagcgcatcg ttcagcccga taacgtgttt gagcaggctg cgcgcagcaa
     1741 tggccgtatt ggaaacggtg gtttcgaggc cttcggggat tttgccgtct tcccacagcc
     1801 aagtcggttc gctgttggtt cgtctgtcgt tttcagacac ccccagactt aaagacggct
     1861 cttccgccaa atggaattgc cattcatgca ggctgtcgag caaggatgcg gcggcttcgt
     1921 cggctaggtt ggcaagttcg gctttatcgg tcagcgcggc aattttgccg gtcagctgcg
     1981 gcagtttttc cagcgtccaa acggcaatat tccatgaatg ttcggcggca aaacggctga
     2041 gggctttttt gggcaggtgg tgcgcttcgt cgatgcaata gaaactgttt tcgggcgcag
     2101 gcagaatcac gccgccgccc atactgatgt cggcaagcag aagatcgtgg ttggcaacga
     2161 cgacatcgac ggtttccaag acatcgcgtg ctaggtaaaa cggacattcc ggacggttgg
     2221 gacaggcggt tttcaggcag ccgtggcggt cgttggtcac tttgagccaa atcgcgtcat
     2281 cgattttttc cggccaagtg tcgcggtcgc cgttgaaccg tcgggcggaa aattcgtcgg
     2341 cgatgtcgcg cagcagcttc aattcttcgg gcttgggttt gctgtcccac aagacggcgg
     2401 gggcttcaaa gccgagcagg ttttgctggg cattgctttg cgtcagtcga tagagtttgt
     2461 aggggcagag atagcggccg cgccctttgg caagtgcgaa ggtcagttcc aaaccgcttt
     2521 tttcgaccag aaacggcagg tcgcggtcta ccaactgctc ctgcaaggca accgtcgcgc
     2581 tgctcacaat cagccgcttg ccgcgtgttt gcgccatgat gccgccggcc aaaaggtagg
     2641 ccaacgattt gcccacgccg gtcggccctt cgatcacggc aatgctctcg ccttcgcgct
     2701 tgggcggctc gccgccttct tcgcgcgcca acgtccgcga aaaagcgttg gcaaccgccg
     2761 caatcatttc ccgctgcgaa gcacgcggac ggaaaccggg caggtttttg ccgatgtttt
     2821 ggtaatggtc gcggatggcg tttttttcta aatcggtgag catggcgttt tgtacggcgg
     2881 tagaagtggg cttattttaa cattgcacgg aagcggtaca atatcgttgt cggaatgggg
     2941 ggtgaggtga atcgtgcgga cgtggttggt tttttggttg cagcgtttga aatacccgtt
     3001 gttgctttgg attgcggata tgttgctgta ccggttgttg ggcggcgcgg aaatcgaatg
     3061 cggccgttgc cctgtgccgc cgatgacgga ttggcagcat tttttgccgg cgatgggaac
     3121 ggtgtcggct tgggtggcgg tgatttgggc atacctgatg attgaaagtg aaaaaaacgg
     3181 aagatattga gtcattcgga cgcaatgccg tctgaaacgg aagttcagac ggcatttgtt
     3241 ttaggttgcc gtaccgctta gggaataccg gcgacaggat gggcgggata gccgtgggta
     3301 tcgaccgaac aggcaaaccg ccaaggcgtg tggacggtgt cggcggacag gtgggcaagc
     3361 tcgggaatgt gccgtctgac aaaggtgccg tcggggtcgg ttttgtgtgc ggcggcggca
     3421 atgtcggggc aggtgtgccg tgaggcggca agccgccagt tgccttggtt gattgctgca
     3481 tcgaaatcgg tcagctgtcg ggcaaaccat atctcgcctt cgcggcgggg gaggtttaaa
     3541 acgtggcaga aaaaatccgc gctcaagcgt ctcagggcgg ggtggaggct gccggttttg
     3601 tgcaaacagc gcatcgcggc atcgataatc ggaatgccgg tccggccctg ctgccaaagc
     3661 gtcaggcgca gggtgtgttc aggattgccg tctgaagggt cgtcatccgt gtgctgcaag
     3721 gcaagttgaa ggaaaaaatc gcggcggatg atgttgtccg cccacgcgtt cagacggcgt
     3781 tcgaggcttt cccgcgcgag caggcgcggc gagatgcagc cggcactcaa atacgcgccc
     3841 atcagcgaag tgtgtttgcg cgaggggaaa tcctttaaaa cggagtagga atccgcctgt
     3901 tcgagaaacc gccgccactg ccgccaagcc gccgtttcgc cgctgttttg cggcaggaag
     3961 atgccgtctg aaagcgcggc aggctgcggg gcggaaaggt tttcggggaa gggttggcgg
     4021 tatgccgcga ataggtccgg accggcgggg ggctgcttgg aaaagcggtc gagccatact
     4081 tcgcggtagc ggtcgaaatc ggcatatgcc gtgccgccgt cgggtatcag gtcggttttg
     4141 ccgaaaacgg cgcggtcgtt gacgaaggtt aacgcgatgc cgtgtttgtc caattcgtgc
     4201 caaagggcgt tgtcggcgag tttgtcggca aaagtatggg attcgtcggc gatgacggtg
     4261 cggatattga ggcggacggc gagccggacg agctcggcag gagatgccgc cgtgtagagc
     4321 gggatgccgc gccctgcaag cccttgggcg agttcggcgg cggattggcg gtagaacgcg
     4381 gcgcggcgag ggttgtctgt ttcggcatcg tcaatccaaa tgccgataat gggcaaacct
     4441 tcggcaacgg cggcgcataa ggcggcgttg tcgcggatgc ggaggttttg gcggaaccag
     4501 acgagcgtgt gtgcggcgca cgtgtccgca taaagggggc gggcggtttc agacggcatt
     4561 tcggcagcct ttcctgctgg cgattttttc gttcagaaaa tcgatgaagc tgcggacttt
     4621 cgcgcttaag aatgccctgt ctgcataaac ggcattcagc cggtcggtcg ggacggcgta
     4681 tccgggcagc agcctcacca gcgtgccgca gcgcaaatcg tgttccgccg cccaaagcgg
     4741 ctgataaccg atgcacgcgc ccgccttaat catttcgcgc atcatcagcg tgttgtcggt
     4801 acggatgacg ggggtcagtt caagccggta ttttttgccg tccgatttgc gggtgaggtc
     4861 gagtttctgc tggttggtgt aggtcggcag gacggcgggc agccccgcca cttcttccgg
     4921 cgtttccggc acgccgttgc gcctcaggaa atcgggcgag gcgagcaggg caaattcgat
     4981 ttccgccagt gggcgcgcaa tcagcgacgg ggacagggtt tgggaaacgc gcaacgccaa
     5041 atccacgcct tcggcaatca aatcgacgtg gcggttgtcc aaaatcagtt ctaatgccac
     5101 ttcgggataa cgttcgcggt attccgccag ccagttgcat atctggctgc cggcaaacca
     5161 cagcggcatc gttacgcgca gcagcccctg cggtttttcc gtccccccgg cggctttttg
     5221 cgcggcatcg tcgagcgtgt cgagcgcgta actgcattgc cggtagtatt cttccccggc
     5281 ttcggtcagg ctgaggttgc ggctgttgcg gtgcaggagt ttggcttgga cggtgttttc
     5341 caagtggctg acgtgtttgc ttgccattgc ggtggagatg ccgagcgcgt cggcggcgcg
     5401 ggtgaagccg ccgctttgga cgacttggcg gaaaaccttg aggctgaaca gggtgtccat
     5461 attttcttgt gtggaaaagt tgtatcaata aaagcagtat atatttgaaa aggggaaaca
     5521 tctatactct accgcctgaa atgaagacaa atatcaaagg agcttttatg tccgattgct
     5581 gcaaccgtat ccaaccggtt ttgctttctg ttttgcgtat cgtaaccgcc tacctgtttt
     5641 tgttgcacgg tacgtcgaaa atcttcgcct tccccattga aatgggcagc ggttcgcccg
     5701 gcgggctgtt gctgcttgcc ggtattttag aaattgtcgg cggcattttg ctggtgttgg
     5761 gcctgtttgc gcgccctgcc gcgtttgttt tgtccggcca gatggcggtt gcctatttta
     5821 tggcgcacgc ttccggaaat gctttgttcc cgattgccaa cggcggcgag tccgcagtgc
     5881 tgttctgctt cgtattcctc tatatcgcgg cggcgggcgg cggagcatgg tcgctggaca
     5941 ggctgttttt caagcgtaaa gcctgaatcg gactgcctaa agtgtatttt gttgaatgtt
     6001 tttgaggaaa agaaatgacc cgtcaatctc tgcaacaggc tgccgaaagc cgccgttcca
     6061 tttattcgtt aaataaaaat ctgcccgtcg gcaaagatga agttgtccaa atcgtcgaac
     6121 acgccgtttt gcacacacct tcttcgttca attcccaatc tgcccgcgtg gtcgtgctgt
     6181 ttggcgaaga gcatgataag gtgtggcaat ttgtcgaaga cgcgctgcgt gccgtcgtgc
     6241 ctgccgacag ttttgaaccg accgcgcaaa aattgaacct gtttaaggcg ggtgcggcaa
     6301 ccattttgtt ttatgaagat caaaatgtcg tcaaaggttt gcaggagcag ttccctgctt
     6361 atgccgctaa cttccccgtt tgggcggatc aggcaaacgc gatggtgcag tatgccgttt
     6421 ggacgacact tgccgcggtc ggcgtaggtg caaacctgca acattacaat cccttgcccg
     6481 atgcggcgat tgccaaagcg tggaatatcc ccgaaaactg gttgttgcgc gcgcaaatgg
     6541 ttgtcggcgg tattgaaggg gcggcaggtg aaaagacctt tgaaccagtt gcagaacgtt
     6601 tgaaagtgtt cggcgcataa tttcgcggtc aaaaaaatgc cgtctgaacc ctgttcagac
     6661 ggcatttttc agtatcaggc ggcgagtttt ccgcattctg agacctttgt ttacaaatat
     6721 catgttcaat atagttaaaa gaaattattc tcatttcctc cgtgaggcaa tataattcgg
     6781 ttgttttgtt aaattgagta taaaaatgaa aatatcattt catttagctt tattacccac
     6841 gctgattatt gcttccttcc ctgttgctgc cgccgatacg caggacaatg gtgaacatta
     6901 caccgccact ctgcccaccg tttccgtggt cggacagtcc gacaccagcg tactcaaagg
     6961 ctacatcaac tacgacgaag ccgccgttac ccgcaacgga cagctcatca aagaaacgcc
     7021 gcaaaccatc gatacgctca atatccagaa aaacaaaaat tacggtacga acgatttgag
     7081 ttccatcctc gaaggcaatg ccggcatcga cgctgcctac gatatgcgcg gcgaaagcat
     7141 tttcctgcgc ggttttcaag ccgacgcatc cgatatttac cgcgacggcg tgcgcgaaag
     7201 cggacaagtg cgccgcagta ctgccaacat cgagcgcgtg gaaattctga aaggcccgtc
     7261 ttccgtgctt tacggccgca ccaacggcgg tggcgtcatc aacatggtca gcaaatacgc
     7321 caacttcaaa caaagccgca acatcggagc ggtttacggc tcaagggcaa accgcagcct
     7381 gaatatggac attaacgaag tgctgaacaa aaacgtcgcc atccgtctca ccggcgaagt
     7441 cgggcgcgcc aattcgttcc gcagcggcat agacagcaaa aatgtcatgg tttcgcccag
     7501 cattaccgtc aaactcgaca acggcttgaa gtggacgggg caatacacct acgacaatgt
     7561 ggagcgcacg cccgaccgca gtccgaccaa gtccgtgtac gaccgcttcg gactgcctta
     7621 ccgcatgggg ttcgcccacc ggaacgattt tgtcaaagac aagctgcaag tttggcgttc
     7681 cgaccttgaa tacgccttca acgacaaatg gcgtgcccaa tggcagctcg cccaccgcac
     7741 ggcggcgcag gattttgatc atttctatgc aggcagcgaa aatggcaact taatcaaacg
     7801 taactacgcc tggcagcaga ccgacaacaa aaccctgtcg tccaatttca cgctcaacgg
     7861 cgactacacc atcggccgtt ttgaaaacca cttgaccgta ggcatggatt acagccgcga
     7921 acaccgcaac ccgaccttag gttacagccg cgcctttact gcttccatcg atccatacga
     7981 ccgagcaagc tggccggctt cgggcagatt gcagcctatc ctcacccaaa accgccacaa
     8041 agccgactcc tacggcatct ttgtgcaaaa catcttctcc gccacgcccg atttgaaatt
     8101 cgtcctcggc ggccgttacg acaaatacac ctttaattcc gaaaacaaac tcaccggcag
     8161 cagccgccag tacagcggcc actcgttcag ccccaacatc ggcgcagtgt ggaacatcaa
     8221 ccccgttcac acactttacg cctcgtataa caaaggtttc gcgccttatg gcggacgcgg
     8281 cggctatttg agcatcgata cgtcatcttc tgccgtgttt aacgccgacc ccgagtacac
     8341 ccgccaatac gaaaccggcg tcaaaagcag ttggctggac aatcgtttgg acaccacatt
     8401 gtccgcctac caaatcgaac gcttcaatat ccgctaccgc cccgacgcgg aaaataatcc
     8461 ctacacttgg gcagtcggcg gcaaacaccg ttcgcgtggc gtggaattgt ccgccatcgg
     8521 gcaaatcatc cccaaaaaac tctatctgcg cggttcgttg ggcgtgatgc aggcgaaagt
     8581 cgttgaagac aaaaaaaatc ccgaccgagt gggcatccat ttgaataata ccagcaacgt
     8641 taccggcaac ctgtttttcc gttatacccg accgaaaacc tctacggcga aatcggcgta
     8701 accggtacag gcaaacgcta cggttacaac tcaagaaata aagaagtgac tacgcttcca
     8761 ggctttgccc gagttgatgc catgcttggc tggaaccata aaaatgttaa cgttaccttt
     8821 gccgcagcca atctgttcaa tcaaaaatat tggcgttcgg actctatgcc gggtaatccg
     8881 cgcggctata ctgcccgggt aaattaccgt ttctgatgaa atcaggcaaa ggctgaaata
     8941 aaactaaaca cattttttca ctcaaatcga acacgccttc aataaaatgc cataaaatcc
     9001 gcacattaat ctgacacaca agagatacct atgaaactga aaaccttagc tttgacttca
     9061 ttgaccctgt tggcattggc cgcttgtagc aaacaggctg aaaccagtgt tccggcagac
     9121 agcgcccaaa gcagctcatc tgctccggca gcccctgctg agttgaacga aggtgtgaac
     9181 tacactgtat tgtctacgcc tattccgcaa cagcaggccg gtaaaatcga agtattggaa
     9241 tttttcggct acttctgccc gcattgcgcc catcttgagc cggtcttgag cgagcacatc
     9301 aaaacgttta aagacgatac ctatatgcgc cgggagcatg tcgtgtgggg tgatgaaatg
     9361 aaacctttgg cacgtttggc ggccgcagtg gaaatggccg gtgaatcaga taaagccaac
     9421 agccatattt tcgatgcgat ggttaatcaa aaaatcaatc tggccgatac cgataccctg
     9481 aaaaaatggc tgtccgagca aacagcgttt gacggcaaaa aagtattggc tgcatttgag
     9541 gctcctgaaa gccaagcgcg tgcggctcaa atggaagagt tgaccaataa attccaaatc
     9601 agcggcacac cgactgtgat tgtcggcggc aaataccaag ttgaatttaa agactggcag
     9661 tccggtatga ccacgattga ccagttggtg gataaagtac gcgaagagca gaaaaagccg
     9721 caataagttg aggattgaat gagtaaaggc catctgaaaa taggatttca gacggccttt
     9781 tgtatttagg ctttatagaa gagatgattg cttaaagcct tatggtttta aatcagaata
     9841 tatag
//
LOCUS       DAWGRZ010000061         9793 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000061 DAWGRZ010000000
VERSION     DAWGRZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9793
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.061"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(9..1304)
                     /locus_tag="WHT44_001077"
     CDS             complement(9..1304)
                     /locus_tag="WHT44_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ2533268.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLGG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAVDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLTYTLGIARAAAEAGAQIFEQSPMTCIEP
                     HQNGWLVYTPEGSVECKNVVYAVNTYAGLNPIFRPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPERMTELVRQDMLKVFPQL
                     ADVKIEYSWGGECDITANLVPHFGRLAPNVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1430..2317)
                     /locus_tag="WHT44_001078"
     CDS             complement(1430..2317)
                     /locus_tag="WHT44_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ2533269.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2317..3282)
                     /locus_tag="WHT44_001079"
     CDS             complement(2317..3282)
                     /locus_tag="WHT44_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ2533270.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTTIDEDLGRLNIAVSYQNYADIFQNFWSTLNPFGDGENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3298..4422)
                     /gene="potA"
                     /locus_tag="WHT44_001080"
     CDS             complement(3298..4422)
                     /gene="potA"
                     /locus_tag="WHT44_001080"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ2533271.1"
                     /translation="MTATTASSAKPYLKIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLGNG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4617..4724)
                     /locus_tag="WHT44_001081"
                     /pseudo
     CDS             complement(4617..4724)
                     /locus_tag="WHT44_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4803..5072)
                     /gene="rpsO"
                     /locus_tag="WHT44_001082"
     CDS             complement(4803..5072)
                     /gene="rpsO"
                     /locus_tag="WHT44_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ2533272.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5295..6230)
                     /gene="secF"
                     /locus_tag="WHT44_001083"
     CDS             complement(5295..6230)
                     /gene="secF"
                     /locus_tag="WHT44_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ2533273.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6234..8087)
                     /gene="secD"
                     /locus_tag="WHT44_001084"
     CDS             complement(6234..8087)
                     /gene="secD"
                     /locus_tag="WHT44_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ2533274.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQFKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPEILLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEMAGGKE"
     gene            complement(8299..8565)
                     /gene="yajC"
                     /locus_tag="WHT44_001085"
     CDS             complement(8299..8565)
                     /gene="yajC"
                     /locus_tag="WHT44_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ2533275.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8693..>9793
                     /locus_tag="WHT44_001086"
     CDS             8693..>9793
                     /locus_tag="WHT44_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ2533276.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKISRL
                     DNDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIISVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2511 a         2611 c         2424 g         2247 t
ORIGIN      
        1 tgcgacgctt aacggctgtc tttccactga taatatttcg agccgaggaa agaaccgagt
       61 ttgcgcaaaa acggttgcag gataatattc ggctggcgca accgctcaaa cggcttcaga
      121 cggcattcgt cccctaaaat tgcttcggca accgccagac ctgcaatgcc tgttatcgcc
      181 atcccgtgtc cggaataacc ttgcgcataa aaaacattcg gggctaaacg tccgaaatgc
      241 gggacaaggt tggcggtaat gtcgcactcc ccgccccacg aatattcgat tttgacatcg
      301 gcaagctgcg gaaaaacttt aagcatatct tggcggacaa gctcggtcat acgctcagga
      361 ttgtcgataa actcgttatc cttaccgccg aaaagcagtc tgccgtccgc gctgaggcgg
      421 taataatcca aaatatggcg gttgtcgcat actgccatat tgttacggat aagccctttt
      481 gcgcgcgccc ccaagggttc ggtcgcaata ataaaggtgc tgacagcaat cgccttgcgt
      541 tccaaaggcc ggaatatcgg gttcaaacct gcataagtat tgacagcata gaccacattt
      601 ttgcactcga cgctgccttc gggcgtgtaa accagccaac cgttttgatg cggttcgatg
      661 cacgtcatcg gggattgctc gaaaatctgc gcaccggctt cggcagcggc acgagcgatg
      721 cccaaagtgt aagtgagcgg atgcaggtgt ccggataagg ggtcgaattg tgccccttgg
      781 tacatatcgc tgtcaagctg ctgtttcaac tcggctttat cccaaagttg ataatgactc
      841 gcaccgtaat gccgttgggc gtgttcatgc cactgctgca actcttccca atgctgcgga
      901 cggacggcaa ccgtggcata accgcgctgc caatcacaat cgacggcatg tttgcggacg
      961 cgttcgtcca ccagttcgac cgcctgcaaa gactgttgcc aaaaccattg cgcctgctcc
     1021 aagccgacct gtttttcaat ttcccccata ccgcaggcgt aatcgctgat aacctgcccg
     1081 ccactccgtc ccgacgcgcc gaaaccgata cgcgcggctt ccaacacaac cgtttcatgt
     1141 ccctgctccg ccaagggcaa tgcagtgcac aaaccaccca atccgccgcc gatgatacag
     1201 gtatcggttt tcagacggca ttgaagtttc ggataaacag tatgaggatt aaccgaactg
     1261 aaataataag aaggcagata ttcttgaaaa tcagggcgaa tcattgtgtt tgctttatca
     1321 ggtgtatttt cggacggaat gatacaggct gtcgggccat atcgtccaaa cagaaaatcg
     1381 gttgaagaaa acaggctgac ccagtcatgc ggtcagcctg ccttattaat taatttgctt
     1441 tctcggcagc caatttttcc tggcggtagg cttctgccgc ttctcggtca cgcttggttg
     1501 cctgcctcat catccaataa ttgacgatga tgaccaatgt tccgatgatg ccgattagga
     1561 tggtcgccaa gacattcatc tgaggatcga gacccaactt gattttggag aaaatcacct
     1621 gcggcaatgt ggatgaaccg gggccggaga ggaatgaggt aatcaccaaa tcatccaaag
     1681 acagggtaat gccgagcaga aagcctgaag cgatggcagg ggcaatcaaa ggcaaagtga
     1741 tgacaaaaaa gattttcagc gggcgcgcgc ccaaatccat tgcggcttct tcgagcgact
     1801 ggtcaagctc aaccagacgc gaacggataa caacggtaat gtacgccata cacagcgtcg
     1861 tatgtccgag gaagatggtg aaaaagccac gatcgaagta gagatgttgt aaccattcgc
     1921 tgccctgcaa aaatatctgt acctgaataa tcagcagcag catagacaga ccggtaatca
     1981 cgtcgggcat caccataggt gcggaaatca tgccagcgaa caaggtactg ccgcgaaaac
     2041 gtttaatccg cgccatcgca tagcctgcca gcgtgcccaa aacgacggcg gcaagcgaag
     2101 acacaacggc aatccgcagc gacagccaag cggcttccaa gatggtgtcg ttttccagca
     2161 atgcgccgta ccacttggtc gaaaagccgc cccaaacggt taccagcttg gattcgttaa
     2221 acgaatagat gaccaaaaca accagcggga tatacagaaa cgccagcgac agtgccaaca
     2281 tcagtttcaa gaaccaagat aatttggatt tctgcattat ttggctcctt cttccaattc
     2341 gcggttttca taatgctgaa acagggcaat cggcacgacc agcagcgcga ccatcacgac
     2401 ggcgacggcg gaagccagcg gccagttgtt ttgatcgaag aacgcctgcc acaagacttt
     2461 accaatcatc aggttttccg aaccgccgac cagctcggga atgacgaact cgccgacagc
     2521 agggacgaaa accagcatgg agcctgcaat aatgccggtt ttcgacaaag gcagggtaat
     2581 cgtcaagaac gatttgaccg gccccgcgcc caaatcggaa gccgcttcaa gcaggcggtt
     2641 gtcgagtttc accagttgcg tgtatagcgg cagaatcata aacggcagat aggcgtaaac
     2701 catcaccaaa ttgagcgaaa aggcattgta gaacaaatcc aaaggctcgc tgataatacc
     2761 cattttaatc aacaggttgt ttacaatgcc gttatgcccg agcagaccca tccacgcata
     2821 gacgcgcaac aggaacgatg tccaaaaggg cagcataatg gcaagcagca aaccattgcg
     2881 gacagaagga ttggcacgag aaatcgcata ggcggtcgga taaccgacca acagacaaat
     2941 taccgtcgta gtcagcgcag tcttaattga agaccaataa gtcatcagat agatattgct
     3001 gttttcaccg tcgccgaacg gattgagcgt actccaaaaa ttttggaaga tgtctgcata
     3061 attttggtag ctgacagcaa tattcagacg acccaaatcc tcatctatcg tcgttaaagg
     3121 agtaaacggc gggatggcga tttcttgttc ggcaaagctg attttcagca cgatggcgaa
     3181 cggaatcaga aacagcacca aaagccaaat atacggtacg gcaatcaccg cacgctgccc
     3241 cggacggcgg aacagtttgt ttttcagttt attaaggttc attgcattcc ccttaaatca
     3301 acggaacaac ggagtcggtt ggttttccgg ccagctgata tagacggttt cgtcccaagt
     3361 cggcggtgta atgttgcgca cataccagta aggggcgggg acttggcttt tgacgacgcg
     3421 cccgttgccg agcttgatat ggtaaatggc gaagctgccc aaataggcga tttcttttac
     3481 cgtgcctttc gcccagttgt agtcgcccaa atattcgggt ttttctttat ataaatcaat
     3541 atcctctggt cgaatactaa cccaaaggtc ctgctcgctc ggaccaccca aaccgtgatc
     3601 gatgcggacg tggttttcca aaccttcgca ttcgataacg gcatagtcgg catgatcttc
     3661 aatcaccaca ccgtcaaaga tgttggtttc gccgataaac tcggcagtga agcggctgtt
     3721 gggatagtcg tacacgtcgc tgggtgtgcc gacttgctgc aactgaccgt cagacataat
     3781 ggcgatgcgg gtcgccatcg tcatcgcctc ttcttggtcg tgcgtaacca taatacaggt
     3841 tacgccgact tgttccagcg tattgaccaa ctcaagctgg gtttgttggc gcagtttttt
     3901 gtccaatgca ccgaggggct catccagcag tagaattttc ggacgttttg ccagactgcg
     3961 tgccaaagca atgcgctgct gctgaccgcc ggacaattgg tgcggtttgc gtttagcaaa
     4021 tttggtcatc tgaaccaggc ggagcatttc ttcgacgcgc gcggcgattt cgcctttagg
     4081 cattttgtcc tgtttcagac cgaaggcaat gttttgttct acggtcatat gcggaaaaag
     4141 cgcgtaactt tggaacatca tattgatggg gcgatcatag ggtgcaagtt tggtaatatc
     4201 ctgaccatca aggataattt ttccctgatt gggactttcc atacccgcca gcatacgcag
     4261 cagtgtagat tttccgctgc cggaactgcc caaaagggcg aagatttcgt gttgataaat
     4321 gtccaagtcg atgttatcga cagcgtaatt gtcaccaaac tttttcacca aaccttggat
     4381 tttgagataa ggtttggctg aagacgcagt ggttgcggtc ataatggcaa tactccaata
     4441 aaaagacgag taccggcaaa acggattttc gaatgggtga taaaaagctg tttgattgct
     4501 ggcgggagtt aaacgtttga tgccgtctga aactcttgta aagcgcacgg gcagcatgaa
     4561 atggaacaag attccaaaga actttatatt atattagttt atgcggtttt cgggcaatat
     4621 agtggattaa atttaaacca gtacagcgtt gccttgcctt gccgtactat ttgtactgtc
     4681 tgcggcttcg tcgccttgtc ctgatttaaa tttaatccac tataaatatg gatttgagct
     4741 tgtcggaaag caacagaaaa gaaaacaccg cccatttttc tgggcggtgt cggaaagcgt
     4801 aattatttac gcagacccaa gcgggtaatc aacgcgcgat acgtatcggg ctgggtacgg
     4861 cgcaagtagg ccagcaggcg gcggcgttgg ctgaccattt tcaacaggcc gcgacggctg
     4921 tggtggtctt tggggttggc tttgaagtgg ggggtcaggt cgttgatgcg gaaagtcaac
     4981 agagcgactt gtacttcgga agagccggtg tcgccttctt tgcgttggaa atctttaacg
     5041 atttgtgctt tttgttctac ggtcagtgcc ataatgaaaa ctccaaaaat ataagaatcc
     5101 ccatagggat tcagacaagt ttgccaagcc tgaagacaac ggcaaactcc ctatgcccaa
     5161 gataggacaa cgtggcatta tgacacaatt cccgcatttc tgcacaatat tttaagaccg
     5221 tggaagtaac cgttaaaaat gccgtctgaa gcattgtctg cttgagacgg caatgttcag
     5281 acggcatatg cgcttcaaac cacgatttct tctttcttct tcggttcttt accgatattg
     5341 tcgcggctca aaccgaacat tagcagaagc gggctggcaa ccaatacgga agaataaatg
     5401 ccgaacacga tgccaatggt caacgccata gaaaagccgt gcaaggccgc accgccgaac
     5461 accagcatgg atacgaccat cgcctcggtc gaaccgtggg taatgatggt gcggctcatc
     5521 gttgcggtaa tcgcgttgtc gatgacttcc ggcacggcat gtccgcgcat cgccggcttg
     5581 cggaagtttt cacggatacg gtcgaagacg acgacggatt cgttcacaga atagcccaat
     5641 acggcaagga tacccgccaa gacggtcagc gaaaattccc attggaagaa ggcaaagcag
     5701 ccgagaataa tcacgatgtc gtgcatattg gcgataatgg cagatacggc aaaacgccat
     5761 tcaaaacgca tcgacaggta aataatgatg ccgataacga caaaacctaa agccatcaat
     5821 ccattactta ccaattcctc accgacttgc gggccgataa attcgacttg gcgcaaggta
     5881 acgtcgggac tgtctttttt cagcaaatcc ataacctgat tggacaactg tgcggaagta
     5941 acaccttctt tgttcggcag gcggatcatg atgtgtttgt tcgtacccaa tgcctgaacc
     6001 tgtacatcac ctattttcag cgtatcgagg cgttcgcgca tcttattgac atccgcaccc
     6061 tgctgatatt ggacttccat taccgtaccg ccggtaaatt cgacagagaa attcagacct
     6121 ctggtaacca aaaagaacac ggcagcgata aacgtaacca acgaaatgaa ggtcgtcagt
     6181 ttgccgtagc tcataaacgg aatatcgcgt ttgattttaa agagttccat agcttactcc
     6241 ttgcctcctg ccatttcggc tttcggcttc cacaccgaac caatggaaat attctgcaat
     6301 ttgcgtctgc gtccgtacca cagattgacc aacgcacgga atacgacgac ggatgaatac
     6361 atcgaagtca gaatacccaa acagtgtacg accgcaaaac cgcgtaccgg gccggaaccg
     6421 aataccaaaa gcgcgatacc ggcaatcagc gaagtcaggt tcgaatcgac aatggtcgcc
     6481 catgcgtgtt ggaaaccgag attgattgcc tgctgcggcg gcacgccggc acgcaattct
     6541 tcgcggatac gttcgttaat caagacgttg gagtcgattg ccatacccaa agtcaacgcc
     6601 agcgcggcca tacccggtaa cgtcaacgtt gcctgcatgg cagacaaaat accgattagg
     6661 aacagtatgt tggcactcaa tgcaatggta gaaaagaaac ccatcagacg atagtaaacc
     6721 accatgaatg cagcaacgat ggcaaaaccc cataaagtcg aatggaagcc tttttcgatg
     6781 ttctccttac ccaaagacgg accgatggta cgttcttcga caatctgcat cggtgcggca
     6841 agagaaccgg cacgcaacag caaagacgta tcattggctt cggctgtcgt catgcttccg
     6901 gaaatttcca cgcgtccgcc ggtaatggca gtacggataa ccggcgcggt tacaacctcg
     6961 gattttcctt ggtcgatcaa aaccatcgcc atgcgtttgc cgacatttgc ggcagtcagt
     7021 tcgccgaaaa tgctgccgcc cgcgctgtcc aagctcagac tgacggcagg tgcgcccatt
     7081 tggtcgaaac tcggttgcgc atcgttgatg ttgtcgcccg tcagctcgac ctgtttgctg
     7141 atcagcagaa tttcgggacg atctccgccg cttgaaagca gctcataacc gctcggcacg
     7201 ttgccttcca atgcctcgcg caacttggca ggatcgtcct ccaccatacg caattccaaa
     7261 gtcgcggtac ggccgatgat gtcttttgcc ttggcagtat cctgaacgcc cggaagctgc
     7321 acgacgatac ggtctgcacc ggactgctgg atgacgggct cggccacgcc caactcgttc
     7381 acacggttgt gcagggtagt gatgttctgt ttgaccgcat cggaacgcac tttattgacc
     7441 gcctcttccg aaagcgtcaa gacgatattg ctgccgtctg aattcagcgt tgcttcagga
     7501 aacagcttgc gcaactgcgg cagagccttt tgcacatcac ctgcatcctg caaagggacg
     7561 gtcaggctgt ttccagcctg acgcaccgtg ccgctgcgga ttttttcgcg gcgcagttcg
     7621 cggcggatgt cgcccgaata acgttcaaac gttttctgca tcgccgcttt catatcgacc
     7681 tgcatggtga aatgcacgcc gccgcgcagg tccaaaccca aaaacatcgg attggctttg
     7741 attttcgcca tccattcggg gctgtccgcc aacaggttga gcgcggtaat atacccttcg
     7801 cccaaagtgt tttcgatgac gtcgcgcgct ttaagctgcg tttctgtgtc tttgaaacgc
     7861 actttcagtg aattgtccac aacaaacatc ccgtcggtct gaatacctgc gtttttcagc
     7921 gcggcatcca ctttgaattg agtctgttcg ttgatgatga tggcttgtcg gttggtcgat
     7981 acctgcacgg cgggtgtttc gccgaatagg ttgggcagcg aatacactgc ggcaaccgca
     8041 atcgtgaaca caatcagcag atatttccat aaaggataac ggttcatcat tgttccttaa
     8101 tggttggaac cccacccttt cggtggtgtc ggaatcgggc tatttcagaa gaggcaaaaa
     8161 cccttcccag ccaggcaaga ccggaaagcg gcatcctgaa tatgccgccc tgcgtgtcgg
     8221 aacatggtca agccttcggt tggaattcaa aacaaagtgc cgcattcggg ctttccagat
     8281 gcggcttgtc ggcacaaatc aatcgacttt tgcggcaatc gcattgcgtt ccacttcgac
     8341 ctcgattttt gtaccctgtc cgatatccac ggtaaaaaac tgttcgccga ctctggttac
     8401 cttacccttg aaacctgccg ccaagaccac tttgtcgccg actttcaagg cggcaagcat
     8461 tgcctgatgc gctttgaatt tcttttgctg cggacgcatg atcaggaagt agaacaccac
     8521 cataatcaac actaaaggag caaattgtgc aacagcttga ttcataattt atccgttctt
     8581 tctaatatgg ttgaaaatcg agaggggtat ataataacat aagaccgtaa acaatatatc
     8641 gggtttgccg tccgtaccga ccgtataccg cagcctgccc gtccacaaac ccatgtcctt
     8701 gacccgccta atcttgaaat tctatgcact gttgcgcctt tttttgggca aaaacgcccg
     8761 caccgcatgg atttcgcatc ccgcctgtgc cgggcacgaa cccggcgcaa accatcccga
     8821 ttcgcccgac cgcatcctct gcatcgagca ggcattgcgc cgcgccggta tttggcagca
     8881 cctccaaacc atagaggcgg aagaaatcag cgatacgcgc ctcgcacttg tccactcgag
     8941 caaatatctg aaccgtttgg aatcttgcct gccccaaaaa ggcaagattt cccgcctgga
     9001 taacgacact gcaatcagca caggatcgct gtctgccgca cgctttgccg ccggttcggc
     9061 agttcaggca gtcgacatgg tcatgaaccg taaagcatgg catgcctttt gcgccgcccg
     9121 cccgcccgga caccatgcgg gcagcggcaa agccggcgga ttctgcctgc tgaacaacgt
     9181 tgccgccggc gtcatgcatg ccattgccga ataccgcctg aaacgcattg ccgtcatcga
     9241 tttcgatgtc cactacggcg acggtacggc agaaatattc aaagacgatc cgcgcatcct
     9301 gtttttcaac ctgtttgaaa ccgacctttt ccccttcccc gaaaacaacg atatgcccga
     9361 cggcggcaat atggtgcacc tgcccttgcc gccaggaacg ggcagccgca cattccgcga
     9421 agccgtccgc aggcagtggc taccccgact tgccgcattc aaacccgaac tggtgctgct
     9481 gtcggcagga ttcgacgcac accgtctaga cgaatcgggc aggctcaacc tgcacgaggc
     9541 ggattttgcc tggctgacac acaaaatcat tcagacggca tcgggctgcc ccggcaaaat
     9601 catatccgta ctcgaaggcg gctacaccct tgaacctctg gcacaatctg ctgccgagca
     9661 tatccgcgtg ctggcagggc tgggcaaatc cgatgccgca accgcctatc agaaaacact
     9721 ggacccaaca aaaaaacggt ttgccaaacc gaaaaccgga caggtgcgcc aacctaccca
     9781 atccgaccgt tac
//
LOCUS       DAWGRZ010000062         9771 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000062 DAWGRZ010000000
VERSION     DAWGRZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9771
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.062"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(313..546)
                     /locus_tag="WHT44_001087"
     CDS             complement(313..546)
                     /locus_tag="WHT44_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ2533277.1"
                     /translation="MKLTLMFREYCSLCHKMRDELKPFQDEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(712..912)
                     /locus_tag="WHT44_001088"
     CDS             complement(712..912)
                     /locus_tag="WHT44_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ2533278.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1084..1959)
                     /gene="xerD"
                     /locus_tag="WHT44_001089"
     CDS             complement(1084..1959)
                     /gene="xerD"
                     /locus_tag="WHT44_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ2533279.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2011..2451)
                     /locus_tag="WHT44_001090"
     CDS             complement(2011..2451)
                     /locus_tag="WHT44_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ2533280.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTLGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            2772..3710
                     /locus_tag="WHT44_001091"
     CDS             2772..3710
                     /locus_tag="WHT44_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ2533281.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKKLLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            3839..4132
                     /gene="hfq"
                     /locus_tag="WHT44_001092"
     CDS             3839..4132
                     /gene="hfq"
                     /locus_tag="WHT44_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ2533282.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            4217..4783
                     /locus_tag="WHT44_001093"
     CDS             4217..4783
                     /locus_tag="WHT44_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ2533283.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALNNTRCRLQEAGYSNVRLILDGHENLKQYIPKPLDAAIFNFGWLP
                     GGDKSLTTRTETSIAALSATLSLLKENGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYGFTNRKNSPPYLLVFEKLRQK"
     gene            4837..5211
                     /locus_tag="WHT44_001094"
     CDS             4837..5211
                     /locus_tag="WHT44_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ2533284.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRFFLLWKNPEKPLAGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            5221..5715
                     /gene="folK"
                     /locus_tag="WHT44_001095"
     CDS             5221..5715
                     /gene="folK"
                     /locus_tag="WHT44_001095"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ2533285.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDTRLTLPHPRAHERSFVIRPLAEILPDFVLGKHGKVAELSKRLGNQGIRL
                     LPDR"
     gene            complement(5841..6320)
                     /locus_tag="WHT44_001096"
     CDS             complement(5841..6320)
                     /locus_tag="WHT44_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ2533286.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDCKDNAYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVFQI"
     gene            complement(6540..7277)
                     /gene="ubiE"
                     /locus_tag="WHT44_001097"
     CDS             complement(6540..7277)
                     /gene="ubiE"
                     /locus_tag="WHT44_001097"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ2533287.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGRLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(7331..7690)
                     /locus_tag="WHT44_001098"
     CDS             complement(7331..7690)
                     /locus_tag="WHT44_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ2533288.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQIADLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEEK"
     gene            7810..9429
                     /gene="mnmC"
                     /locus_tag="WHT44_001099"
     CDS             7810..9429
                     /gene="mnmC"
                     /locus_tag="WHT44_001099"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ2533289.1"
                     /translation="MDNLVWDGIPDIRTLDQAIRKHAPPLNLIICLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQAMQCLQFDSINLIEHILPDVRFWLVPPSRTHHLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITSAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPAFVRTLLNHPLIGLHEDTPLTDISHDGEKWIASTPNGTFTATHIIYCTGANSPY
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALSESLFAANPNPQKHQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAIAAQILG
                     LPHPFSQRLRHALHPNRTIIRAIVRRKDLTP"
BASE COUNT         2602 a         2984 c         1965 g         2220 t
ORIGIN      
        1 ctaccgatta cacaaacaca tatcatcttt acacaatcat gcttccatca acagtaaaac
       61 atgatatgat tgccaacaat aacatctcac aataaatttt ctaattttta ttgaaaaaat
      121 caataaataa gaatcctccc aaccgacaaa taaaataaag aaagggttaa tatgcaacat
      181 cgaagaagac tagcaattta ccaagcatcc aaacgtgctt cctttaccgg caggtcatcc
      241 gccccgcaaa aacgtaaaga acgttgattt gaaaaaaatg ccgcctgaag tcctgcttca
      301 gacggcattt ttttaccgtt cgagaaactg tttcaacctg tcctcatcca aaaaccagtg
      361 acagatttcc tcatctcccg ccaacaaaac gggaaccagc tcattgtatt tttcttccaa
      421 aacaggattt tcatccacat cgaccacttc cagcccgaac ccgtattcat cctgaaaagg
      481 tttgagttcg tcgcgcattt tgtggcacaa gctgcaatat tcacgaaaca tcaaggtcaa
      541 tttcatccgc gtttccttat ctgtcaattt gcacacgcca aagccttaga cgcagcagaa
      601 tcatggtcta tttgggaaaa aacaatgttt tcgaggaaga tgatactcaa gtcctgccaa
      661 aaacagtaaa aatgccgtct gaacagttca gacggcattt cgaaaaccgt tttacgcttg
      721 aacgttgata cccgccgtaa ccgtcggttg cgcattgtgc gccgtcagaa acgaagcggc
      781 aagctccccg cagcagccgc agcagctcgc tacgcccaat tgcgactgca aatcgcccat
      841 tgtggtcgcg ccggcggcga tggtttcctt gatttgatgg tcggtaacgg cattgcagat
      901 gcagacaaac attttgtgct ccgtgtgttc tcaaactatc gtaccgatag cggcttttat
      961 tatcgtatgc gaatataaat aaaaacggtt cgcattgcaa ggtcggtata cacggtttgt
     1021 cttggtaatt ttttatcaag tttgcatttt cgattgattt cttattaaac caaagtagaa
     1081 gcctcagttt cgggaatggt gttccttcac tacaccctgc aaccatacgt tggcaacatg
     1141 ggtataaatc tgcgtcgtat tcaaatcggc atgtcccaac atatcctgaa ccacgcgcaa
     1201 atccaagccg tgccgcacca gatgcgtggc aaaggcgtgg cgcaggctgt gcgggctgat
     1261 gtgcccgatg cctgcctgac ttgcatattc tttgacaatc atccatgcca actgacggga
     1321 aatgcccgtc tttttctgac tgacaaacaa tgcgtcgcaa ttcctgcctt tcagcagaag
     1381 tgggcgtgcc tccgtataat agcgttccac ccaatacgcc gactcctgcc ccatcgggac
     1441 catcctctgc ttatcaccct ttcccagcgc ggtaatacag cccctgtcca aatccacatt
     1501 gccgaagttc agcccgaccg cctcgctgac gcgcaagccg gtcgcgtaca tcaattcgag
     1561 caaagccttg tcccgcaaac cgtgcggcgt gtcggtatcc ggggcggcaa gcagtcggga
     1621 aatctgctgc tcggtgatca gggtcggaat attcttgtcg attttgggcg gtttcagcaa
     1681 acgggtggga ttgtccgtcc ttatgccttc acgctccatc catatataca ggcgtttgca
     1741 tgccgataat gcgcgcgcct gcgaactccg ttgctctccg tcaacataaa ccgccgccgc
     1801 caaatccgct tcgtccgcat ccttcagcat tctgcccgat tgggacaggc ggcgggcgat
     1861 tttttccaaa tcgcgccggt aaccgtttaa agtattctga ctgagccgcc tgtccaacca
     1921 cagcgtttca agcagcctgt cgatcaaacc ttcttccata ccgttccaaa caaatgccgt
     1981 ctgaatcttc ttcagacggc atggttcaca ttatcgggaa agcgtttcca atacttcctg
     2041 cgcgtgaccc gccactttga ctttccgcca ttcatgggcg atttctccat ccttattcaa
     2101 gacgaacgta ctgcgctcga tacctaacga ctctttcccg tacagtttct tcaatttgat
     2161 gacatcaaac aggcggcaca ctgtttcatc cttgtcgctc aacagctcga accggaaacc
     2221 ctgcttggcg caaaaattct gatgcgcctt tacgccgtcg cgggaaatac cgaccacggt
     2281 ataacccaat gcctcaaact gttccaaacg cgcattgaaa tccaagcctt ccgtcgtaca
     2341 gcccagcgta ctgtctttcg gataaaaata cacgaccaaa ggcagatgtt ctgccgaatg
     2401 aaaatccgca ccgctgctcg aaggcagggt aaattcatat ttcacatcca tagtcctact
     2461 cccgatattc ccattattca aaacggcacg cagacgcacc gccgcaattg ccaaaccaac
     2521 cccgattcta ccgccccaaa ggacaaggat tcaaccgccg gaaacatcca aaccgacaca
     2581 cgacggcatg aaaaatatcc atgtcaaacc acaaaatatg ttccgattta aaaacagaat
     2641 gttataaaac ccaatcccca aaacacaaca agaccgcccg cctacgggca gtctcctgtc
     2701 agacgacata ctttacagat ggctgttttt tcaacaaaat aacgccaata ctcaaaaata
     2761 tggaatcaaa aatgtccatc catactctga aacgcctgcc ctcatcgctg ctgctcggtc
     2821 tctgcctttc cctgccgtca gcccaccttt ttgccgacaa cgacatttta gggcaatttt
     2881 tagaacagaa catgcttacc tcctccgatc cgatagaaat attcgccgaa agcacgatac
     2941 accccaccaa cacccaagcc attacaggcg gtctgattct ctcctcacag tctgccctgg
     3001 tcgtcaacaa caaaaccgga cagatactgt atcagaaaaa cgccgacagg attatgccca
     3061 tcgcctccat ttccaaactg atgagcgcga tggtcgtttt ggatgcaaac ttggacatga
     3121 acgaaaccgt taccattacg cccgacgaaa tcgaccgcat caaagggacc ggcagccgtc
     3181 ttgccatagg tacggcactt acacgcaaaa aactgctgca cctgagcctg atgagcagcg
     3241 aaaaccgcgc cacccatgca ttgggcagaa cctaccccgg cggcatgggc gcatttgtcg
     3301 ccgccatgaa ccgcaaagcc caaagcctcg gtatgtacgg cagccgcttt tacgaaccga
     3361 ccggactcaa cttccaaaac gtttctaccg ccaaagacct gagccttatg gtcaacgccg
     3421 ccgcccaata tccgcaaatc cgcaccaact cgacttccaa ctacgcctcg gtacagacca
     3481 aaaacgggca gcagaactac aaaaactcca atgccctggt cagagaaggc atgtggaaca
     3541 tcgaattgca gaaaaccggc tacatacgcg aagcaggcag gtctatggtt gtcaaagcca
     3601 acattcaaaa ccaacccgtt accatcgtat tgctgaactc gcccacatcc gccacacgcg
     3661 tcaacgacgc ccgcaaaatc gaatcgtgga tgctgcagca acgctcctga catacaaatg
     3721 cccggcggaa aaccgcccaa acacaaaaaa aattggtaat ttttcctatt caaggctaca
     3781 attcgacacg cacagggcgt ctgcccgatt tcacaatcat aataacggag ttaaaaatat
     3841 gacagctaaa ggacaaatgt tgcaagatcc cttcctgaac gccctgcgta aagagcacgt
     3901 tccggtttcg atttacttag ttaacggtat caaattacaa ggtcaggttg agtctttcga
     3961 tcaatacgtt gttctcctga gaaacacttc cgtcacccaa atggtttaca aacacgccat
     4021 ttccaccatc gtaccggcac gctccgtcaa cctacaacat gaaaacagac cccaagccgc
     4081 accgacttcg accctcgtcc aagtggaaac cgtccagcag cctgccgaat aatccgcacg
     4141 aagcatgacg tgtcatatct ttcaatacct taccggacaa cggtaaggta tttttatttt
     4201 cagacagcat ttaaaaatgt tattgcaaaa catccttcca ttcgcccatt gccttttgcg
     4261 gaaggcactt cccgaaggtg gcaatgcttt ggacggcacc gccggcaacg gacacgacac
     4321 ccttttcctc gcacaaaccg caggcatccg ggggaaagtg tgggcattcg acatccagcc
     4381 gcaagccctg aacaacaccc gatgccgtct gcaggaagca ggttacagca atgtacggct
     4441 catcttggac ggacatgaaa acctgaagca atatattcca aagccgctgg atgcagccat
     4501 tttcaatttc ggctggctgc ccggcgggga caaaagcctt accacccgca cggaaaccag
     4561 cattgccgcc ctttctgcca ccttatccct actgaaagaa aacggtatgc ttattgccgt
     4621 cctctatccg ggacacgaaa acggcaaaca ggaggcagaa gcaatcgaac aatgggcaaa
     4681 aaacctgcct caagaacagt ttgccgtttt gcgttacggc tttaccaacc ggaaaaacag
     4741 cccaccctat cttttggtat ttgaaaaact gcgtcaaaaa taactgtttg cggtaaaata
     4801 agccgattct aacccttatt ttgatacgag ccgaccatgc agtatctgat tgtcaaatac
     4861 agccaccaaa tcttcgttac catcaccatt ttggtattca acatccgttt tttcctactt
     4921 tggaaaaatc cagaaaagcc cttggcgggc ttttggaagg cactgcccca ccttaacgac
     4981 accatgctgc tgtttacggg attgtggctg atgaaaatta cccatttctc cccgttcaac
     5041 gcgccttggc tcggtacaaa aatcctgctt ctgctcgcct atatcgcatt gggtatgatg
     5101 atgatgcgcg cccgtccgcg ttcgaccaag ttctacaccg tttacctgct cgccatgtgt
     5161 tgcgtcgcct gcatcgttta ccttgccaaa accaaagtcc tgcctttctg aaacaccgtt
     5221 atgaacaaca gacattttgc cgtcatcgcc ctgggcagta atcttgaaaa ccctgcccaa
     5281 caggtacgcg ccgcattgga cacgctgtcg tcccatcctg acatccgtct taaacaggct
     5341 tcctcactgt atatgaccgc gcccgtcggt tacgacaatc agcccgattt tgtcaatgcc
     5401 gtctgcaccg tttccaccac tctggacggc attgccctgc ttgccgaact caaccgtatc
     5461 gaggctgatt tcggacgcga acgcagcttc cgcaacgcgc cgcgcacatt ggatttggac
     5521 attatcgact ttgacggcat ctccagcgac gacacccgac tcaccctgcc gcatccgcgc
     5581 gcgcacgaac gcagtttcgt catccgccct ttggcagaaa tcctccctga ttttgtttta
     5641 ggaaaacacg gaaaggttgc cgaattgtca aaacggctgg gcaatcaagg tatccgtctt
     5701 ttaccggaca ggtaattccg cacgcggatg ccgtctgaaa gcctttcaga cggcattttt
     5761 cctttgccgc caacacgcgt gcaaaaaaat cgccccttgg aaaagggggc gcaaaaggaa
     5821 cacaaaccac taccaaaact ttaaatctga aacactgcct gccgcatact gtatccgaca
     5881 ggatataaag ccctcactaa atcgtttcga gaaatccaaa cttcttcatc gccgacagaa
     5941 aatctgcctt tctccggtac cagctccaac agaaacggtt gaaccgccgt atgcagcctg
     6001 tccttacacc gccccagctt tctggacaac gcggacaggg cgcgtttgta ggcattatcc
     6061 ttgcagtcaa gctcccgcgc actgccgacc cagctcaaac gaaggttgcg gctttcatcc
     6121 tcaatcagca aaatgcctgt tttgacttcc cccacctcgg gctcgcatga aagcgcaata
     6181 taatatttgc cgtcgatgtt gacatacgcc gcttcatgca cctgacagtt gcagccggaa
     6241 gtggcaagca gcgaataaac agaatcccga tgcagtttca aaatatcaag ggaaatctgt
     6301 ttgaaaatac tttccggcat ttttccctcc tttcgtctgc tgcgttgttt actggattga
     6361 ctgcattata cagacatctc ccaatcttgc aaatgataat tgttcctatt ttttaaaaaa
     6421 tttataaatt attgatggat atgtgaatta attttaagat ttaaaatctc tttctcaaaa
     6481 aaacacaaaa atgccgtctg aaacgtgttc agacggcatt ggctgcacag ccagtccgtt
     6541 taaaatttca cgcccttatg cagcgcgacg atgcccgcac tcatattgtg ataatccacg
     6601 ctgtcgaagc ccgcatccag catcatctgt ttcaaagttt cctgatcggg gtgcatacgg
     6661 atggattcgg caagatactg gtaactctcc gcatctttcg caatcagcct gcccatgacc
     6721 ggcagcagct tgaacgaata gaaatcatac gcgccttcca aaggtttgta gattttggaa
     6781 aactccaaca ccagcaacgt gccgcccggt ttcaaaacac ggtacatctc tttcagcgcg
     6841 gcatctttat gcgtcatgtt ccgcaagccg aacgccacgg aaaccaagtt gaaataattg
     6901 tcggggaaag gcagtttttc cgcatcggca agcgatacgg gcaaaatcat gccttcgttc
     6961 aacagacggt cgcgcccgac ggtcagcata gaggaattaa tatcggtcag ccaaacctcg
     7021 ccttccttgc cgacccgttt cgcccaaccg cgcgacaaat cgcccgtacc gcccgcaatg
     7081 tccaacactt tatcgccttt tttcaggtgc gccgtgttga tggtgaaatg cttccacacc
     7141 ctgtgcagcc ctgccgacat cacatcgttc ataatgtcgt agtttttggc gacggagtgg
     7201 aacacttccg ccactttgcc ggctttttca tcttcgttga ccgtactgaa tccgaaatgc
     7261 gttttctgtc cgcccatatt ctaaccacct ctctgatacc tttgcccgtc agattaccat
     7321 ccgaccggtc ttatttctct tcaaaacgcg acgcgcccgc cgccgccaat ttgtccaaat
     7381 attcctgcca catcgcatcg tatccgtatg ccagtctgtg cagatacgcc caatcgtaaa
     7441 gaccgctgtc gtgcccgtct gaaaaactga ttttcagcgc gtactgtccg acaggctgca
     7501 aatccgcgat ttggacatcc gccttgccgg tctgcaaaac atcctgtccc acgccgtgtc
     7561 cgcgcacttc cgcactcggc gaatagacgc gtaaaaattc ggcaggcaga tttttaggtt
     7621 cgtccccata agtcaaaacc aacacggtac ggtttttcaa caaacggatt tcaacaggaa
     7681 tattattcaa aaaacacctc ggttcggcat acaatatccg gcaggcgcag gcgtgccttc
     7741 aaacggcgcg tattttaaca gctttatccc gataccgata ttcaaaccca cactttaaac
     7801 ggcattgata tggacaatct cgtatgggac ggcattcccg acatccgcac actcgaccaa
     7861 gccatccgca aacacgcacc cccgctcaac ctgattatct gcctccccga taatcagatt
     7921 cccgattttc aaaccgcaca agatgcttcg gacgcggaat gccgtctgaa gcaccgtttg
     7981 gatcaggcaa tgcagtgcct ccagttcgac agcatcaacc tcatcgaaca catcctgccc
     8041 gatgtccgct tctggctggt tcccccttca cgcacccacc acctgcacga acatttccac
     8101 cacatttcct ggcagaccga agccatcccg caaaccgaaa gcaagcccga caaaccctgg
     8161 tttgcacttc cacaaacatc cgaacggcaa aaaccggaac acatcctcgt tatcggcgcg
     8221 ggcatatccg gcgcggcaac cgcccacgcc ttagcatcac acggcatttc cgttaccgta
     8281 ttggaagccc gaaaagccgc ccaagccgcc agcggcaacc gccaagggct gctctacgcc
     8341 aaaatctcgc cgcacgacac cgaacagacc gaacttttgc ttgccggcta cggctacacc
     8401 aaacgcctgc tcggacacat cctgcccgaa tccgaaacct ggggcggcaa cggcatcatc
     8461 cacctcaatt acagccgcac cgaacaacaa cgcaatcacg aattgggttt gcaaaaacac
     8521 cataaccacc tctaccgcag catcacatct gcagaagccg aaaaaatcgc cggtatccca
     8581 ctgtccgtcc catacgacca cccttcatgc ggactctact ggcaacacgg cgtatggctc
     8641 aatccacccg cattcgtccg caccctcctc aaccatccgc tcattggact acacgaagac
     8701 acacccttga ccgacatttc ccacgacggg gaaaagtgga ttgcaagcac gccaaacggc
     8761 acatttaccg ccacacacat catctactgc accggtgcga acagccccta cctacccgaa
     8821 accaacctcg ccgccctgcc tctcaggcaa atacgcggac aaaccggcct cacaccgtcc
     8881 accccgtttt ccgaacaact gcgttgcgcc gtttcaggcg aaagctacat cagcccgtcg
     8941 tggcacggac tgcactgcta cggcgcgagt tttattccca acagcagcca taccggatgg
     9001 aacgaagccg aagaagcctc aaaccgccaa gcattggcac accttaaccc cgccctttcc
     9061 gaatcattgt ttgccgccaa cccaaacccc caaaaacacc aagggcacgc cgccatacgc
     9121 tgcgacagcc ccgaccacct tcccctagtc ggcgcactcg gcgacattgc cgccatgcgg
     9181 cagacctaca ccaaactcgc gctggacaaa aactaccgca tcgacacccc atgcccatac
     9241 ctgcctaatg cctacgtcaa caccgcgcac ggcacccgcg gactcgccac cgcccccatc
     9301 tgcgccgccg ccattgcagc ccaaatccta ggcttgcccc atcccttttc acaacgcctg
     9361 cgccacgccc tacaccccaa ccgcaccatc atccgcgcca tcgtcagaag gaaggatcta
     9421 accccttaag tctttgccgc atgccgtccc gaccacagaa aatcatattc aaataaacat
     9481 accaatcctg acggtaaaat atcgaaaaca cccataacgt tttcttccca aaataaggag
     9541 taaggaatgc ctaacaaatt gcctttgctg tccaagatga atgttccgta acattcgaag
     9601 acttccttaa ccctgccagc cgtttcggtt tctgtacccc ctgttagata aggaaggaat
     9661 gaaaaagccc tgttgattgt atgaagtaaa gggcgttaat ttttgttaat cttcccttct
     9721 caggaactca atatataagg atgttaattt ttatttccct tctcgaggac t
//
LOCUS       DAWGRZ010000063         9724 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000063 DAWGRZ010000000
VERSION     DAWGRZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9724
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.063"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(420..1343)
                     /locus_tag="WHT44_001100"
     CDS             complement(420..1343)
                     /locus_tag="WHT44_001100"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ2533290.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
     gene            complement(1901..2305)
                     /locus_tag="WHT44_001101"
     CDS             complement(1901..2305)
                     /locus_tag="WHT44_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ2533291.1"
                     /translation="MPSTCRDAASAVCTKTPEYYPNHGEWPKNNTSAGVASSSTIKGK
                     YVKEVTVANGVITATMLSSGVNKEIQGKKLSLWAKRQDGSVKWFCGQPVTRNAANAKA
                     GTDEVTAAAAANGKKIDTKHLPSTCRDASDAS"
     gene            complement(2624..2951)
                     /locus_tag="WHT44_001102"
                     /pseudo
     CDS             complement(2624..2951)
                     /locus_tag="WHT44_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(2980..3309)
                     /locus_tag="WHT44_001103"
     CDS             complement(2980..3309)
                     /locus_tag="WHT44_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ2533292.1"
                     /translation="MGGLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(3387..3605)
                     /locus_tag="WHT44_001104"
     CDS             complement(3387..3605)
                     /locus_tag="WHT44_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ2533293.1"
                     /translation="MLKTLTFAALHFSVAFSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YFHEKAWNLYEKNKTVKQTQPFQLHRCS"
     gene            3727..4680
                     /locus_tag="WHT44_001105"
     CDS             3727..4680
                     /locus_tag="WHT44_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ2533294.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHASAVREGYVSFEDAVRAADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTNEPPKNGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(4755..6812)
                     /gene="metG"
                     /locus_tag="WHT44_001106"
     CDS             complement(4755..6812)
                     /gene="metG"
                     /locus_tag="WHT44_001106"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ2533295.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHACH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGTKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVADSELLAKLTAQSEAIAECYESREYAKALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AAKFLNLEAITWANTRDTLGKHAINKYEHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EEGKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDTGAQPGD
                     KVG"
     gene            complement(6928..8766)
                     /gene="glmS"
                     /locus_tag="WHT44_001107"
     CDS             complement(6928..8766)
                     /gene="glmS"
                     /locus_tag="WHT44_001107"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ2533296.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGLYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKDAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPSDVEIASEYR
                     YRSVIADSDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGKLSPVVHTIPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            9048..9569
                     /locus_tag="WHT44_001108"
     CDS             9048..9569
                     /locus_tag="WHT44_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533297.1"
                     /translation="MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKE
                     KGNYFLNKIHVVTGKEESLLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYRDARNALPSNQTYQQHLAAIEQLKRRFEAEFDELEK
                     EIKCNGRSPALLL"
BASE COUNT         2113 a         2343 c         2802 g         2466 t
ORIGIN      
        1 aacagcaata ttcaaagatt atctgaaagt ccgggattct agattcccac tttcgtggga
       61 atgacggcat cagtctgccg tttacagcac ggtttcttta gattttacgt tctagattcc
      121 cgcctgcgcg ggaatgacga atccatccat acgaaaacct gcaccacgtc attcccacga
      181 acctacatcc cgtcattccc acaaaaacag aaacctcaaa tcccgtcatt cccgcgcagg
      241 cgggaatcca gacttgtcgg tgcggacgct tatcggataa aacggtttct tgagattccg
      301 cgtcctggat tcccactttc gcgggaatga cgaattttag gtttctgttt tggttttttg
      361 tccttgtagg aatgatgaaa atttaagttt taggaattta ccggaaaaaa tagaaagcgt
      421 tatccacaag ttctgatgtt cagctcgtga aatgcgtcgg gcaaatcatc gctgtcggca
      481 aattccaccc ggtcgtaagc cgtttcgtct gccaaaaccg cgcgcaagag tgcgttgttg
      541 atggcgtgtc ccgatttgta gccttcaaat gcgccgacaa tcggatgtcc gacgatatac
      601 aaatcaccga tggcatcaag gattttgtgg cgcacaaact catcgggata gcgcaagcct
      661 tcaggattca ggacatccgt gtcgtcaatc acgatggcgt tgttcaaatt gccgcccaaa
      721 cccagattgt gggcgcgcat catttccact tcgtgcataa agccgaaagt gcgcgcgcgc
      781 gcgatttcgt cgatgtagga tttgccggcg aaatcgattt caaaagtggg cgagctgcgg
      841 ttgaaaaccg gatggtcgaa ttcgatggtc agcgttacct taaagccgtc atacggcgta
      901 aagcgcaccc atttgcccgc ttctttgatt tcgacaggct tgaggatttt caaaaaacgc
      961 ttttgcgcct tttgatcgac cacgcccgca tcttgcaaaa ggtaaataaa cggcaggctg
     1021 gagccgtcca taatcgggat ttcgggcgcg ttcagctcaa tcagcgcatt gtcgatgccg
     1081 taggcggaca gcgcggacat aatgtgttcg atcgtgccga cgcgcacgcc tttgtcggta
     1141 acgatggtgg aggaaaggcg ggtatcgttg atcaaataag gggtcagctt gatttgttcg
     1201 cccatctcgc cgtccaaatc ggtacggcgg aaggaaatcc cgctgttttc aggcgcgggg
     1261 tgcagggtca gcgcgacgcg ttcgcccgaa tgcagcccga cgccggtaac gctgatggat
     1321 ttcgccaaag ttctttgcag cataaaccgc ttccttatca agggggtaag ttttggaata
     1381 atacgataaa accggaaaaa caggctatgt ttttccatag tatttgccaa tgtatccgtt
     1441 ttcaatacgt aagccgcata aaaatgtata gtggattaac aaaaatcaag acaaggcgac
     1501 gaacccaccc ccctcctgaa aaacgcaaaa aatgccgtcc gaaaaccttt cggacggcat
     1561 tttcgcgtaa accgtcattc ccacaaggac aaaaaaccaa aacagaaaac caaaaacagc
     1621 aacctaaaat tcgtcattcc cgcgcaggcg ggaatctagt ccgcttgttc ggtaaatgag
     1681 agggcggatt gcgcgcctgt cagataaacc acgtgtttaa acgggcggca atgaggtacg
     1741 cgcagagcct tgaagcgcaa tcgatatatt attttcagcc aaaacggacg cccccgcttg
     1801 ccttgcaaac ctttaaaaag gaagccaccc ggattaatcc gagtggccgt ggaaaatcac
     1861 ttaccgcttg atttatttaa aatttatggt ataatttacc ttagctggca tcacttgcgt
     1921 cgcggcaggt tgacggcagg tgcttggtgt caatcttctt accgttggcg gcggcggcgg
     1981 cggtgacttc gtcggtgccg gctttggcgt tggcggcgtt gcgcgtaacc ggctgtccgc
     2041 agaaccattt taccgaaccg tcttgacgct tggcccacag ggagagtttt ttgccttgga
     2101 tttctttgtt tacgccgctt gaaagcattg tggcggtaat gacgccgttt gcgactgtaa
     2161 cttccttaac atatttgcct ttgattgttg aagaagatgc cacgccggca gaagtgttgt
     2221 ttttcggcca ttcgccgtga ttcgggtaat actcgggtgt ttttgtgcaa acggcagatg
     2281 ctgcgtcgcg gcaggttgac ggcaggtgct tggtgttggt tttcttgttg ccggtgttgt
     2341 cggcggcgac ggtgtcgtcg gtgccggcgc gcgtaaccgg ctgtccgcag aaccatttta
     2401 ccgaaccgtt ttgacgcttg gcccacaggg agagtttttt gccttggatt tctttgttta
     2461 cgccgcttga aagcattgtg gcggtaacga cgccgtttgc gactgtaact tccttaacat
     2521 attttccttt gattttagag gaggatgcca cgccggcggc atcattaaat cccgtcattc
     2581 ccacgaaagt gggaatctag aactcaggac cggagaaacc tttttacccg ataagtttcc
     2641 gtgccgacag acctggattc ccgcctgcgc gggaatgacg aagtttttcg gccattcgcc
     2701 gtgattcggg caatactcgg gtgttttgtg caaacggcag atgctgcgtc gcggcaggtt
     2761 gacggcaggt gcttggtgtc aatcttctta ccgttggcgg cggcggcggc ggtaacgtcg
     2821 tcgttggcgg ctttggcgtt gtcgcgctca accggctgtc cgcagaacca ttttaccgaa
     2881 ccggcttgac gcttggccca cagggagagt tttctgcctt tgatttcttt gtttacgccg
     2941 cttgaagcca ttatgtcaga cggtattgcc cgggcagctt tattcgtaca ctttcagcag
     3001 ctcgacttca aatatcaaag tggcgtgcgg gggaatcacg ccgcccgcgc cgtgtgcgcc
     3061 gtagcccatt tccgaaggga tggtcagctt gcgtttgccg ccttccttca tgccgccgaa
     3121 gccttcgtcc cagcctttga tgacttgtcc gacaccgagc gtgatggtca gcggctggcg
     3181 gcggtcgagg ctggagtcga atttggttcc gttttccagc caacctgtgt aatgcacggt
     3241 aatctctttg cctttaactg cttcttttcc gaagccttct tgcaagtctt caataatcag
     3301 gccgcccata tttgtccttt cgttgcttgt tggtcaaaac ggcaagggta acataccgtc
     3361 cgtcgaagtc aaatgccgct caaacgtcag ctgcatcggt gcagctgaaa cggctgtgtt
     3421 tgtttgactg ttttattttt ttcgtaaagg ttccatgctt tttcatggaa atagaaaacg
     3481 acggtgttga ttaggggttc gaccagcgca actgctcccg atacgcctat actgcccgtc
     3541 agtacatagg ttacactgaa ggcgacgctg aaatgcagtg cggcaaaagt cagggtttta
     3601 agcatcatcc tctcccggat tggacattga cggagagatg ataaagatta tcataaggct
     3661 gcgcggttta aatttgctat ttgttgttag tgtagataaa tcgtttttta aataaggata
     3721 ggaattatga atcataaaaa gatcgttgtt ttggatgcgg atactttgcc cggccgggtt
     3781 tttcattttg attttccgca cgagcttgcg gtttacggta cgacaggtgc ggatgaaacg
     3841 gcagaacggg tgcgcgatgc acatattgtc attactaaca aagtgatgat ttctgccgat
     3901 attattgcgg ctaatccgca gttggagctg attgccgtca gtgcgaccgg cgtgaacaat
     3961 gtcgatattg gggcggcgaa ggcggccggt gttgcggtat gcaatgtccg cgcatacgga
     4021 aacgaatcgg ttgcggaaca cgcctttatg ctgatgattg cgttaatgcg gaatttgcct
     4081 gcctatcagc gtgatgttgc ggcaggattg tgggaaaagt cgccgttttt ctgccattac
     4141 ggcgcgccga ttcgggattt gaacggcaaa acgctggcgg ttttcggacg cggcaatatc
     4201 ggacggacgc ttgccggata cgcgcaggca ttcggtatgg gggtggtgtt tgccgaacac
     4261 aaacacgcgt ccgctgtgcg tgaaggctat gtttcctttg aagatgcggt acgggctgct
     4321 gatgtgttgt cgctgcactg tccgctaaac gcccaaactg aaaatatgat aggcgaaaac
     4381 gaattgcggc agatgaagcc tggcgcggtt ttaatcaatt gtgggcgcgg cgggctggtg
     4441 gatgaaaacg cgctgcttgc cgcactcaaa tacgggcaga tcggtggggc aggtgtcgat
     4501 gttttgacga atgagccgcc caaaaacggc aatcccttgc tgaatgcacg attacccaat
     4561 ctgattgtta cgccgcatac cgcgtgggca agtcgtgagg ctttggacag gctgtttgat
     4621 atattgttgg cgaacattca cgcctttgtg aaaggagagg cgcaaaaccg cgtggtttga
     4681 acctgtcggg attgcggaaa aaaatgccgt ctgaacgcct caagggttca gacggcattt
     4741 cttgagattc ccgtttaacc gactttgtcg cccggctgcg cgcctgtatc cacatccaag
     4801 agcttcagtt tcccgtctgc cgtggcggca ctcaaaatca tgccttcaga tacaccgaat
     4861 tttgccattt tgcgcggggc gaagttggcg acggcgatga ccatgcggcc gttcaattcg
     4921 gcagggttcg ggtaagacgc ggcgatgccg gagaagatga tgcgtttttc aaaaccgaaa
     4981 tcgaggtcga atttcaaaag tttggtgctg ccttcgacag cttcgcagtt caatactttg
     5041 gcaacgcgca tgtcgatttt cataaagtcg tcgaaactcg cctgttcggc gactttttcg
     5101 tatttgccct cttcggcggc aggtgcggct gcggcggcga tgctttgttt gttggcttcg
     5161 attaaatcgt ccacttgttt ttgctccact cgttgcatta aatgttcgta tttgttgatg
     5221 gcgtgtttgc ccaaggtatc gcgtgtattt gcccaagtga tggcttccaa attcaggaat
     5281 ttggcggcgt ttgcggcggt ttgcggcaag acgggggcga ggtaggcggt caacatggtg
     5341 aaggcgttga tgagttcgct gcatacttcg tgcaggcgtt cgtcttggcc ttcttgtttg
     5401 gcgagttccc acggcttgtt ggcatcaacg tattcgttga caatgtctgc caaggccatg
     5461 atgtcgcgca gggctttggc gtattcgcgg ctttcgtagc attcggcaat ggcttcgctt
     5521 tgcgcagtca gttttgccag caattcgctg tcggcaacat ctttcagacg gccttcaaag
     5581 cgtttggcga tgaaacctga ggcgcgggcg gcgatgttga cgtatttgcc gacgaggtcg
     5641 ctgtttacgc ggctgataaa gtcttgcagg ttcaaatcga tgtcttcgat tttgctgttg
     5701 agtttggcgg cgatgtagta gcgcatccac tcggggttca ggccttgttc cagataggat
     5761 ttggcggtaa taaacgtgcc gcgcgatttg gacatttttt gtccgtcgac ggtcaaaaag
     5821 ccgtgtgcgt acacgccggt cggggcgcgg tggccggaga aatgcagcat agcgggccag
     5881 aacagggcgt ggaaatagag aatatctttg ccgatgaagt ggtacatctc ggtttggctg
     5941 tcggctttga agtattcgtc aaaatcgacg ccgatgcggt cgcacaggtt tttaaacgac
     6001 gccatgtagc cgacgggcgc gtccagccag acgtagaagt atttgcccgg cgcgtcgggg
     6061 atttcaaaac cgaaatacgg cgcgtcgcgg gaaatatccc agtcggacag ggtggtttct
     6121 tcaccttcgc ccagccattc tttcattttg ttgagggctt cggcttgcag atggggcttg
     6181 ccgtcgtgcg ggttgttgcc ggaagtccat gctttgagga agtcggcgca ttcgcccagt
     6241 ttgaagaaga agtgttcgga ttcgcgcaat tcgggtttcg taccggaaac ggcggaatac
     6301 gggttaatca gttcggtcgg ggaataggtc gtgccgcaga cttcgcagtt gtcgccgtat
     6361 tggtcttggg cgtggcattt cgggcattcg cctttgacga agcggtcggg caggaacatt
     6421 tgtttttcgg ggtcgaaaag ctgctcgatg acgcggctct caatcttgcc gttggctttc
     6481 agcgcgcggt aaatgtcttg ggaaaactgt ttgttttcag gggaatgggt gctgtaataa
     6541 ttgtcgtaac cgatgaaaaa gccagtaaag tcggcgaggt gctcttcgcg cactttggca
     6601 atcatgtctt cgggcgcgat accttgtttt tgcgcggcaa gcattacggg cgtgccgtgg
     6661 gtgtcgtcgg cgcagcagta gtggcacgcg tggccgcgca gtttttgaaa gcgcacccaa
     6721 acgtcggttt ggatgtgttc gaccatgtgg ccgaggtgga tgctgccgtt ggcatagggc
     6781 agggcggagg taactaagat tttgcgtgtc atattgtgct ttgcaaacaa tgggtaaagg
     6841 cggattatac cgcaaatcaa acggggaaat gccgtctgaa gcctgaaaaa tcgggcttca
     6901 gacggcattt ttgccaaccg gcgggagtta ttcgacggtt acggatttcg ccaggttgcg
     6961 cggcttgtcc acatcggtac cgcgtgcgag ggcggtgtgg taggcgagga gctgcacggg
     7021 gatagtatgc acgacggggg acagtttgcc gacgtggcgc ggtgcgcgga taacgtgcac
     7081 accttcggtg gcattaaaat tgctgtcgag gtcggcaaag acgaaaagtt cgccgccgcg
     7141 cgcgccgact tcctgcatat tggctttgac tttgtccaac aggctgtcgt tgggtgcgat
     7201 gacgacgacg ggcatatttt cgtccaccag ggcaagcggc ccgtgcttca gttcgccggc
     7261 aggataggct tcggcgtgga tgtaggtgat ttccttcagc ttcaacgcac cttcgagggc
     7321 aatcgggtaa tggatgccgc gccctaaaaa cagcgcgctg gttttcttgg caaactgttg
     7381 cgcccatgcg gcaatttgag gttcgaggtt cagagcgtgc tgcacgctgc cgggaagctg
     7441 gcggagttct tcggtgtaac gcgcttcgtc ttcttcggaa accaaaccgc gcactttcgc
     7501 cagcgttacc gccaaaccga acagcgcaac cagttgcgtg gtaaacgctt tggtcgaggc
     7561 gacgccgatt tccgcaccgg cacgggtata aagcacgagg ctgctttcgc gcggcagggc
     7621 ggattccatc acgttgcaaa tggagaggct gtggcggtgt cccaaggatt tggcgtattt
     7681 caacgcctcc atcgtgtcca gcgtttcgcc ggattgggaa atggtaatga ccagttggtc
     7741 ggaatcagca atcacgctgc ggtatcggta ttcgctggcg atttcgacgt cggacgggat
     7801 ttttgcgatg gattccaacc aatatttggc ggtcagcgcg gcgtaatagg acgtgccgca
     7861 ggcaaggatt ttgacgctgc ggatgctttc aaacacgctt ttggcatctt tgccgaagtt
     7921 ttcggggatg aagccgccgt cgaggaaaac ctccgccgtg tctgcaatcg cgcggggctg
     7981 ctcgtggatt tctttttgca taaagtggct gtacagtccc agttccaaag aggcgagcga
     8041 gagttcggat accttgactt tgcgttcggc aggcaggccg tttttatcgg tcagcctttt
     8101 gatgccgtct gaagccagca gcgcgatgtc gccgtcttcg aggtacgcca cgcggcgcgt
     8161 aaaggcgatg acggcggata cgtccgaagc gataaaggtt tcatcgtcgc ccaaagcgac
     8221 caaaagcggg cagcccatac gcgccacaac taattcatca ggcttgtctt gggcaataac
     8281 cgcgatggcg tatgcgccgt ggaaacgttt gaccgcttct tgtaccgctt caaacagcct
     8341 gccgccgttt tgcgcgtatt cgtgattgat gctgtgtgcg atgacttcgg tatccgtttg
     8401 cgattcaaaa cggtatccca aaccttccaa acgtttgcgt tcgctttcaa agttttcgat
     8461 gatgccgttg tgtacgaccg caatcatacc gccgctgatg tgcgggtggg cgttcggctc
     8521 agtaacgccg ccgtgtgtcg cccaacgcgt atgtccgatg ccgatgccgc cgctgatgcc
     8581 tttttcgcgt gccgcgtcct ccataagctg cacgcgtccg acgcggcgca cacgtttgat
     8641 tttgccgtcg gtgttgacgg caatgcctga tgagtcataa ccccggtatt cgaggcgttt
     8701 gagaccgtcg gtcagaaaat cgacgacgtt gtgatgggcg cggatggcgc cgacgatacc
     8761 gcacataact gttccttagt atccggttga aaaaaaacag gcgcggacgg cttccgtgcc
     8821 gcaccttcct cttcggatta taaaccgcct cccgcgccgg aaaacagcaa aatgccgtct
     8881 gaaggcttgg gcttgctcaa aaaaaggagg gatttccctg tttatccagg atgggcgttc
     8941 agacggcatt acctgctgct ggtttatagt ttttgcaaat caacattgac aagctgaaaa
     9001 aaaaaaacaa tatactcgct cggtcttaat gttaacggag tatggaaatg aaacaaatgc
     9061 ttttagccgt cggcgtggtg gcggtgttgg cgggctgcgg caaggatgcc ggcggttacg
     9121 agggttattg gcgcgaaaag tcggacaaaa aagagggtat gattgccgtc aaaaaagaaa
     9181 aaggcaatta cttccttaat aaaatccacg tggttacagg caaggaagag tccttgcttt
     9241 tgtctgaaaa agacggcgcg ctttcgataa acacagggat aggggaaatc ccgatcaaac
     9301 tttccgacga cgggaaagag ctgtatgtcg aacgtaggca gtatgtcaaa accgatgcgg
     9361 cgatgaagga caaaatcatc gcccatcaga aaaagtgcgg acaaacagca caggcatacc
     9421 gcgacgcgcg aaatgcgttg ccgtcaaacc agacgtatca gcagcatctg gcggcgatcg
     9481 agcaattgaa acggcggttt gaagccgagt ttgacgaatt ggaaaaagaa atcaaatgca
     9541 acggcagaag cccggcattg ttgctttagt aggggacaac cgggaggatg ccgccgtccg
     9601 aatcggatgt gcggtttctg taccggtacg ggcgggcagg aatgtccgcc ttttttgttc
     9661 ggatgcgttt gaatacccgt ttgattccga ccgtttgcaa ggggtatttc cgttcgggcg
     9721 gaaa
//
LOCUS       DAWGRZ010000064         9470 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000064 DAWGRZ010000000
VERSION     DAWGRZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9470
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.064"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            89..298
                     /locus_tag="WHT44_001109"
     CDS             89..298
                     /locus_tag="WHT44_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ2533298.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            387..1601
                     /locus_tag="WHT44_001110"
     CDS             387..1601
                     /locus_tag="WHT44_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ2533299.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1688..4267)
                     /gene="clpB"
                     /locus_tag="WHT44_001111"
     CDS             complement(1688..4267)
                     /gene="clpB"
                     /locus_tag="WHT44_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ2533300.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDILPSRELQAVLNLMDKAATKRS
                     DAYIASELFLLALVQQNDATGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQDNIRNIAKIQLKGLEKRLEKQNLRLAV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            4528..5538
                     /gene="trpS"
                     /locus_tag="WHT44_001112"
     CDS             4528..5538
                     /gene="trpS"
                     /locus_tag="WHT44_001112"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ2533301.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNLDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDTPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFRELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAAKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5607..6152
                     /locus_tag="WHT44_001113"
     CDS             5607..6152
                     /locus_tag="WHT44_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533302.1"
                     /translation="MLKTSFAVLGGCLLLAACGKSENTAEQPQNAVQSAPKPVFKVKY
                     IDNTAIAGLDLGQSSEGKTNDGKKQISYPIKGLPEQNVIRLIGKHPGDLEAVSGKCME
                     TDDKDSPAGWAENGVCHTLFAKLVGNIAEDGGKLTDYLVSHAALQPYQAGKSGYAAVQ
                     NGRYVLEIDSEGAFYFRRRHY"
     gene            6472..6795
                     /locus_tag="WHT44_001114"
     CDS             6472..6795
                     /locus_tag="WHT44_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533303.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKM
                     KDAAK"
     gene            complement(7004..8512)
                     /gene="ppx"
                     /locus_tag="WHT44_001115"
     CDS             complement(7004..8512)
                     /gene="ppx"
                     /locus_tag="WHT44_001115"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ2533304.1"
                     /translation="MTTTPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(8629..9360)
                     /locus_tag="WHT44_001116"
     CDS             complement(8629..9360)
                     /locus_tag="WHT44_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ2533305.1"
                     /translation="MSREINAGRHHFLYGGISGGGVHYWDNKDFSEQSLRLSFGYKNR
                     SVTRSFGIVPFVEQNLLGGSRYNFVGGFNADFSQRLSERWRLTLNAGNMWKHYQEDRT
                     AARYDSHMPLAGATLMYSAPKDWLLYGGADWSHNITKEAEQASIRKGLRVGAVKTFDG
                     GLGLRANLRYTRRMFDAPGTIVYRFPRKDHEYQANLSLWHDKISWKGFTPQLNFRYLK
                     IDSNMKSFYTRKNMQIFMSVEKDFK"
BASE COUNT         2200 a         2444 c         2500 g         2326 t
ORIGIN      
        1 gatttgaatt tttcagaaaa tgaagcggac gtttgggcgg aggcaacttg tttgataaga
       61 tagcaatatt ttaaaacgga gaaagatcat gccttatgtc aatattaaag taaccggcgg
      121 caaggaagca ccgactgccg cgcaaaaagc ggaactgatc ggcggcgtaa ccgaattgct
      181 ggcacgcgtg ctgggcaaaa atcccgaaac aacggttgtc gtgattgacg aagtggatac
      241 cgataactgg ggaataggcg gcaaaagcgt cagcgaacgg cgcaaagagg gcaggtaaaa
      301 agcctgaaaa tctcggtttg atgctttaaa ttccgcgtga aaaagagtac attcccaccc
      361 attgcccaaa atttacggaa cacatcatgg ataaatttcc caagtctgca aagctcgatc
      421 acgtctgtta cgacatacgc gggccggttc acaaaaaagc ccttcagttg gaagaggagg
      481 gcaataaaat ccttaaactc aatatcggca accctgcgcc gttcggcttt gaagcccctg
      541 atgaaatctt ggtcgatgtc atccgcaacc tgccgacttc gcaaggctat tgcgattcta
      601 aagggctgta ttccgcccgc aaagccattg ttcactacta tcagaccaaa ggtttgcgcg
      661 atattacggt tgatgatgtc tatatcggca acggcgtgtc cgagctgatt acgatgtcta
      721 tgcaggcatt gctcaacgac ggcgacgaaa tcctgattcc cgcgcccgac tatcccttgt
      781 ggacggcggc ggcaacgctt gcgggcggta cggtacgcca ttatctgtgc gacgaagaaa
      841 acggctggtt ccccaacctt gccgatatgg aagccaaaat cacgcccaaa accaaagcca
      901 tcgtcgtcat caatcccaat aatccgacag gcgcggtgta cagcagggaa atcctgttgg
      961 aaatcgccga actggcgcgc aagcacggtt tgattatttt cgccgacgag atttacgaca
     1021 aaatccttta tgacggcgcg gttcaccacc acatcgccgc gcttgccccc gaccttttga
     1081 cggtaacgtt caacggtttg tccaaagcct accgtgtagc cggattccgc caaggctgga
     1141 tggtgctcaa cgggccgaaa catcatgcaa aaggttacat cgagggtttg gatatgctct
     1201 cgtctatgcg cctgtgtgcc aatacgccga tgcagcacgc gattcagacg gcattgggcg
     1261 gctatcagag catcaacgaa ttcattttgc ccggcggacg gcttttggaa cagcgcaaca
     1321 gggcgtggga actggtcaac cagattcccg gcgtatcctg cgtcaaaccg atgggcgcga
     1381 tgtatatgtt cccaaaaatc gataccgaaa tgtaccgtat ccgcgatgac atgaaattcg
     1441 tttacgattt gctggtgcgc gaaaaagtct tgctggtgca gggaacgggg tttaattgga
     1501 tcaagcccga ccatttccgc attgttacgc tgccttacgt ccatcagatt gaagaggcga
     1561 tgggcaggct ggcaagattc ctgcaaacct accgccaata aggggacggt ttgtctgccg
     1621 aggataaaaa atgccgtctg aaacggagat tcccgtttca gacggcattt tcaacagcag
     1681 gaacgaatca ggcaaatttc agtctgtcgc cgtcggcttc caccctgatt tcgctttcgg
     1741 gcgcatagtt tccggcaagc agggcttttg ccagcgggtt ttcgatttcc gactggatgg
     1801 cgcgtttgag cggacgtgcg ccgtaaatcg ggtcgaaacc ggctttggcg atgatgtcca
     1861 gtgcggcatc ggaaacagcc aggcgcaggt tttgtttttc caaacgtttt tccaagcctt
     1921 tgagctggat tttcgcaatg ttgcggatat tatcctgatc cagtccgtgg aacacgacca
     1981 cttcgtcgat gcggttgatc atttcggggc ggaaatgttc tttcacatcc tccatcacaa
     2041 cttctttcac cgcttcgtaa tcctgaatgc ccatttgttg gatatgttgg ctaccaatat
     2101 tggaagtcat cacgataacg gtatttttga agtccacggt gcgaccttgt ccgtcggtca
     2161 agcggccgtc atccaatact tgcagcagga tgttgaacac atcgggatgg gctttttcca
     2221 cttcgtccag cagaatcacg ctgtacggtt tgcggcgcac ttgttcggtc aggtagccgc
     2281 cttcttcgta gccgacatag cccggaggcg cgccgattaa gcgggcaacg gcgtgttttt
     2341 ccatatattc ggacatatcg atgcgaatca gatgatcttc gctgtcgaac agaaagcctg
     2401 ccagggcttt acacaactcg gttttaccca cgccggtcgg gcccaagaac aggaagctgc
     2461 cgtaaggctt gttcggatcg gcaagaccgg agcggctgcg gcggatagcg tcggacacgg
     2521 cacgcacggc ttcgtcctgt ccgaccacgc ggcggtgcaa tacttcttcc attttcagca
     2581 gtttgtcgcg ttcgccttcc atcattttgg atacgggaat gccggtcata cgggaaacca
     2641 cctctgcgat ttcctctgcg ccgacattat tacgcaagag tttgtttgcc ggttttgtgc
     2701 tgtccgtatc tgcccgttcg gcggctgcac gctgtttttc caaatgctcc aaatcttcat
     2761 acatcaattt tgaagccagt gccaaatcgc cttgccgttt tgcctgttcg attttaattt
     2821 tgacttcgtc aatttgtttc ttaatattag cagcaccgtc tgaaattgct ttttcggctt
     2881 tccagatttc gtctaaatcg gcgtattctt tttgcagacc gttgatttcc tcgtctatca
     2941 gttccaaacg ttttttgctg gcatcgtctt tttctttttc aacgtgcgcc ttttccatcc
     3001 gaagctgaat tagacggcgg tcgattttgt ccattgcttc cggcttggtt tctttttcca
     3061 tcttgacacg gctggcggct tcgtcaatca aatcaatcgc tttatcgggc aggaagcggt
     3121 cggtaatgta gcggtcgctc aactccgctg cggcaacgat agcagggtcg gtaatatcga
     3181 taccatggtg gatttcataa cgctcctgta aaccgcgcaa aatagcgatg gtgtcttcca
     3241 cgcttggctc gccaaccaat actttttgga agcggcgttc gagtgccgca tctttttcga
     3301 tgtattggcg gtattcgtcc aaagtggtcg cgccgataca gtgcaattcg ccacgtgcca
     3361 aagccggttt cagcatattg cccgcgtcca tcgcgccgtc ggttttgccc gcgccgacca
     3421 aagtatggat ttcatcaatg aaaatcagag tgttgccgtc gtctttcgcc aaatcgttca
     3481 acacgccttt caagcgttct tcaaattcgc cgcggtattt cgcgccggca atcaaagccg
     3541 ccaaatccaa aaccagcaag cgtttgttac gcagggattc aggtacttcg ccgttgacga
     3601 tacgttgcgc caagccttca acaatggcgg ttttacccac acccggctca ccaatcagca
     3661 cagggttgtt tttggtacgg cgttgcaata cctgaatcgc gcggcggatt tcgtcgtcac
     3721 gaccgataac ggggtcaagt ttgccgtcgc gggcgcgctg ggtcaggtca agcgtatatt
     3781 ttttcaaagc atcgcgttgg tcttcggcat tggcatcgtt cacgttttgt cctcctcgta
     3841 ctgcgtcaat cgcggcattg atgttttgtt cggtcgcgcc ggcttctttc aaaattttgc
     3901 cggtcgcatc gttctgctgt accaaggcaa gcaggaaaag ttcgctggca atataggcat
     3961 cgctgcgttt ggtggcagct ttgtccatca ggttcaacac cgcctgcaat tctcggctgg
     4021 gcagaatatc gccgccctga ccggacactt tcggcaggct gtttaaatgc tgctgcaaac
     4081 gctgtttcac ctgcggcacg ttcacgcccg catgagccaa gagcgcggcg gctccgctgt
     4141 tttggtcgtc aagcagggct tttaacacaa agcccgcttc cagatagctg ccgtccgcag
     4201 ccaacgccaa actctgagct tctgcaaggg cttgttggaa tttggcggtt aatttgtcgt
     4261 atcgcatttt tgtttccttt tcaaaatgtc cgctgtcgaa gcctatatgt gcataattgt
     4321 ggataactca agttctgttt tctgtttttc tatatttaat tcgatatatc attgaattta
     4381 aagtatataa aaatgtataa taatgtgtat aactatatct tcttaatatg gaaaagtctg
     4441 ttgtcggctg gatgtaggtg gcaaatcggg tataatcggc acatcttttt ccctttcaga
     4501 cggcattgat gccgcaagga catttttatg agcaaaaaac gagttctgac cggcgtaacc
     4561 accaccggca tcccgcatct gggcaactac gtcggcgcca tccgccccgc cgtccgcgcg
     4621 gcgcaaaacc tcgataccga atccttcctc ttcctcgccg attaccacgg tatcatcaaa
     4681 tgccacgagc cggagatgat tcaccaatcc acccaagccg ttgccgccac ttggcttgcc
     4741 tgcggactcg accccgagcg caccaccttc taccgccaaa gcgacactcc cgaagtgatg
     4801 gaattgaact ggattctgac ctgcatcact gccaagggtt tgatgaaccg cgcccatgcc
     4861 tacaaagccg ccgtgcaggc aaatgcagaa aacgggcagg aagaccctga tttcggtgtg
     4921 gaaatgggtt tgttcagtta tccgattctg atgactgccg atattctgat gttcaacgcc
     4981 aacgaagtgc ccgtcgggcg cgaccaaatc caacacgtcg aaatggcgcg cgacatcgcc
     5041 ggccgcttca accaccgctt ccgggaactc ttcaccctgc ccgaagtgaa aatcgatgaa
     5101 aacgtcgaac tcttggtcgg tttggacgga cgcaaaatgt ccaaatccta cggcaacacc
     5161 attccgcttt gggaaaacga caaaaaaacc caaaaatcgg tcaacaaaat catcaccaat
     5221 atgaaagagc cgggcgagcc gaaacagccc gacgaaagcc cattgtttga aatctacaaa
     5281 gccttctcca cgccgtctga aacggtggaa tttacgaaaa tgcttgccga cggcttggcg
     5341 tggggtgaag ccaaaaaact tttggcggcg aaaatcaacg ccgaactcgc cgaaccgcgc
     5401 gaacgctaca acgagctgac cgccgaccct tcgcaaatcg aagagatttt gcaggcaggc
     5461 gcggcgaaag cgcgtaaaga agcacgcgaa ttattggaca aagtacgcga tgcggtcggc
     5521 atccgcccgt tgaaatgaac ccgatgccgt ccgaacctcc gcctgccgcg tttcagacgg
     5581 cattttgaaa ccatcaggag tgtggaatgc tgaaaacatc ttttgccgta ttgggcggct
     5641 gcctgctgct tgccgcctgc ggcaaatccg aaaatacggc ggaacagccg caaaacgcgg
     5701 tacaaagcgc gccgaaaccg gttttcaaag tcaaatatat cgacaatacg gcgattgccg
     5761 gtttggattt gggacaaagc agcgaaggca aaaccaacga cggcaaaaaa caaatcagtt
     5821 atccgattaa aggcttgccg gaacaaaatg ttatccgact gatcggcaag catcccggcg
     5881 acttggaagc cgtcagcggc aaatgtatgg aaaccgatga taaggacagt ccggcaggtt
     5941 gggcagaaaa cggcgtgtgc cataccttgt ttgccaaact ggtgggcaat atcgccgaag
     6001 acggcggcaa actgacggat tacctagttt cgcatgccgc cctgcaaccc tatcaggcag
     6061 gcaaaagcgg ctatgccgcc gtgcagaacg gacgctatgt gctggaaatc gacagcgaag
     6121 gggcgtttta tttccgccgc cgccattatt gaggtattcg gacatcccgg aatatatttg
     6181 ggtttttcaa accctgcagg aaaaagtccg caccgtcggg aaactcaaaa ggagggggat
     6241 atgtgttaca attttccgaa ctgttttcat aaaatagttt tcggacgtgt ttcaatatgg
     6301 cattgatgcc gccttattgt tcgagaaaaa aacctttata tttaaatata atgggtttta
     6361 actaaacggg aaaccgtttt ctctccggtc gatgggcaaa atcagccgat tgatggaaca
     6421 cggtccgatt tttaagcaaa accttaatca acaactcaag aaggataaga catgaaaaaa
     6481 ttattgattg ccgcaatgat ggcggctgcc ttggcagctt gttcgcaaga agccaaacag
     6541 gaggttaagg aagcggttca agccgttgag tccgatgtta aagacactgc ggcttctgcc
     6601 gccgagtctg ccgcttctgc cgtcgaagaa gcgaaagacc aagtcaaaga tgctgcggct
     6661 gatgcaaagg caagtgccga ggaagctgta actgaagcca aagaagctgt aactgaagca
     6721 gctaaagata ctttgaacaa agctgccgac gcgactcagg aagcggcaga caaaatgaaa
     6781 gatgccgcca aataatttgt tgccttggca aaaatgatgg gatgccgcct gccggcaacc
     6841 caaacgaaac cgcctgaaga ttttcaggcg gtttttgggt tatgtgcccc ttgtttttta
     6901 cgcctgcatg accgttccga gcagatgtgc cgcgcatatc gggatgttgc ggacaggcaa
     6961 atgccgtctg gacagggttt ggacggcatt tgttccgcaa ggttcagacg gcctcgactt
     7021 tgaacggcat attgattttt tgccattgga cgctttcata ttccaatgcg tcggcaatca
     7081 ggggatggcg ttccagccat tccctgtcaa tacgcaggat gaagccgcag ctttccgtat
     7141 ccgtgcgcaa ctgcatattt ttcgggaaag acaggtcttg gcgcgaacgg cagaacagtg
     7201 cggcaaggcg cagggacaaa acggcatacc acaacatttc gttggtgccg atgatgccgc
     7261 tcattttttt catatcgccg cgatgaccga tgaccagttg ggcaagtatg gtctgttctt
     7321 tgcgtgagaa acccggcata tcggcgtttt cgaggatgta ggcggaatgc ttgtgatagc
     7381 cggtgtgggc gatgtccaaa ccgatttcgt gcagcgcggc ggcgcgtccg agatactgtt
     7441 gccacaaggc aagctcttga actgtaacgt ttttagcgtg gcagaggctg tccataaagg
     7501 tttgcgcggt ctcggcggtg cgtttcgcct gattgaggct gacgtggtag cggtgttgga
     7561 actcggcaac cgtttgtccg cgcatatctt cgtttaaacc gcgcccgatc aaatcgtaaa
     7621 acacgccgtc gcgcagggcg gcttcggtta cggtcatcct gtcgagtttc atttcctcaa
     7681 acgccgccat catcacggca agtccgccgg caaaaacttc gatgcgttcc ggtttcaggt
     7741 tttcaaattt ggcttttttg accgaaccgg cttcgatgat gcgttcggcg agggcgcgca
     7801 tgcctttgta ggtaatgtcc gcctcttggg gcatttcggc ggcaagcacg tcgcggatgg
     7861 attttgccga acccgatgtg ccgacggcga aatcccaacc ttcgcgcctc atatttttgc
     7921 tgatacgctg gatttcgttg cgggcggcgg aaatggcaga ttggaagtct ttggcggtga
     7981 ttttgttttg gaagaagcgc aggctgtagg ttacgcagcc caagggcagg ctttcggtaa
     8041 tgtcgggatt cagcgtcgag ccgatgacaa attctgtcga accgccgccg atgtcgataa
     8101 ccagcatttt gccgccgccc ggggggaggg tgtggatcac gccggtataa atcagccgcg
     8161 cctcttcgcg cccggcgatg atttcgatgg ggaaacccaa tgccgcttcg gctttgggaa
     8221 ggaaatctgc gatgtttttg gcaacgcgga atgtgttggt tgccacggcg cgtacctgtt
     8281 cagggcggaa gccgcgcagg cgttcgccga attttgccag acagtccaaa gcctgttctt
     8341 gggaagcggc actcagattt ttctgttcgt ccagtccggc ggcgaagcgc accatctgtt
     8401 tgaacgaatc gatgactttt aattgtccgt tgttgttttc gcaaatctgg aggcggaaac
     8461 tgttggaacc caaatcgacg gaggcgagga cgtttgcggg ggtggtggtc atggcggata
     8521 ccggtggggg aaaaacgcaa tgttactctg acggcgcagg cgttgacaat aaatgatgcg
     8581 gcggtttttg attctgccca cggatgttgc cgacggcatt ttttgcgctt atttgaaatc
     8641 cttttccacg ctcatgaaaa tctgcatgtt tttgcgtgtg taaaaacttt tcatattgct
     8701 gtcgattttc agatagcgga aattgagttg cggcgtaaag cccttccaag agattttgtc
     8761 atgccacaac gacaggtttg cctgatattc gtggtctttg cgcgggaagc ggtacacaat
     8821 ggtcccgggt gcgtcaaaca tcctgcgggt atagcgcagg tttgcccgca gacccaagcc
     8881 gccgtcgaac gttttgaccg cgccgacacg caaacccttg cggatggaag cctgttccgc
     8941 ctctttcgtt atgttgtgcg accagtccgc accgccgtaa agcagccagt ctttcggcgc
     9001 ggaatacatc agcgtcgcgc ccgccagcgg catatggctg tcgtatcggg cggcggtgcg
     9061 gtcttcctga taatgcttcc acatattgcc cgcgtttagt gtcaaccgcc agcgttcgct
     9121 caagcgttgg gagaaatcgg cattgaagcc gccgacgaaa ttgtatcggc tgccgcctaa
     9181 gaggttttgc tcgacaaacg gcacgatgcc gaacgagcgc gttaccgaac ggtttttata
     9241 gccgaacgac aggcgcaggc tctgttcgct gaaatctttg ttatcccaat aatgcacgcc
     9301 gccgccgctg atgccgccgt agaggaaatg atgcctgccc gcattgattt cgcgcgacac
     9361 gcctaagccc tagcgcaaac cgtgtgcctt ttgcggcagg ctgtcggcgg ttttcgtcca
     9421 gcttctgccc gcaaattcaa tcgttttttc ggacgaagcg ttgtttatag
//
LOCUS       DAWGRZ010000065         9323 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000065 DAWGRZ010000000
VERSION     DAWGRZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.065"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(91..1371)
                     /locus_tag="WHT44_001117"
     CDS             complement(91..1371)
                     /locus_tag="WHT44_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ2533306.1"
                     /translation="MENSLKEAALKFHEFPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKQFYAIEDNGQRVLADAVKGKDIFLGLSGANLLTPEIL
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            complement(1593..2213)
                     /locus_tag="WHT44_001118"
     CDS             complement(1593..2213)
                     /locus_tag="WHT44_001118"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ2533307.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEEVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLKPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRGVYLDRAAACPERYAVIDSNRNLDEVRNSIEKVLDGHFGC"
     gene            complement(2273..3268)
                     /gene="mltG"
                     /locus_tag="WHT44_001119"
     CDS             complement(2273..3268)
                     /gene="mltG"
                     /locus_tag="WHT44_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ2533308.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIGHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLQIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLVEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            3352..3924
                     /gene="ampD"
                     /locus_tag="WHT44_001120"
     CDS             3352..3924
                     /gene="ampD"
                     /locus_tag="WHT44_001120"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ2533309.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFGGREKCNAFSIGIELEGCDFEPFAEAQYRSLEALLDAICRHYPVTAVTGHQD
                     IAPGRKTDPGHFFDWRRIREKGFLVDRNAV"
     gene            4142..5428
                     /locus_tag="WHT44_001121"
     CDS             4142..5428
                     /locus_tag="WHT44_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ2533310.1"
                     /translation="MIYIVLFLAVVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGSVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            5576..8101
                     /gene="ligA"
                     /locus_tag="WHT44_001122"
     CDS             5576..8101
                     /gene="ligA"
                     /locus_tag="WHT44_001122"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ2533311.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHICRLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGAFDHAEMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMLKADFVALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQDGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEHMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTVVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMRETAVAVSDGIGHRQDDLFAET
                     PSANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESDQMPSEG
                     SSVGASGKHKKQPVKWAENILAGIEASKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPP
                     ARHAEPQRWIARLPGFKISENKAQALWELAGKNIEGLQTDKALPTDWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            8158..>9323
                     /locus_tag="WHT44_001123"
     CDS             8158..>9323
                     /locus_tag="WHT44_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ2533312.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGALEGAGFVHYETSAFAKPAMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLNDLLQ"
BASE COUNT         2179 a         2835 c         2364 g         1945 t
ORIGIN      
        1 ttgtcctgat ttttgttaat ccactatatc aatctgacag caaaatgccg cctgaaaaca
       61 gttcagacgg cattttaaac cgcaaacggc ttacagcttc cattcgctca gcttggcagc
      121 gtaagcttcc aaatctgcaa tcggacgggt tgccacaccg ctttccatcg ctgctttggc
      181 ggcagccgta gcaacgcgcg gcagcaggcg ggaatcgaac ggagtcggaa tcaggtattc
      241 cgcgccgaat tcaaatttct taccgtaagc ggcaaccact tcttcggtta cttcttccat
      301 cgccaaatcc gccaaagcat acacgcaggc gcgtttcatt tcttcgttga tggtcgtcgc
      361 gccgacatcc aacgcaccgc ggaagatgaa cgggaagcac aatacattgt tcacttggtt
      421 cgggaagtcg gagcggccgg taccgataac cacgtccgga cgggtttctt tcgccagcgg
      481 cggcaggatt tccggattcg ggttggccat agcgaacacg atgggttttt cgttcatggt
      541 gttcagtatt tcaggcgtca gcaggttcgc gccggagagg cccaagaaga tgtctttgcc
      601 tttgacggca tcggcaagca cgcgctggcc gttgtcttca atggcgtaga actgtttgga
      661 ctcgtccata cggtctttgt cttcgcgggt ttggtaaatc acgcctttgg agtcgcaaac
      721 ggtcacgttt tcgcgtttca agcccaaatc cagcaattgg ttcaagcagg caatcgcggc
      781 cgcacctgcg ccggaacaca ccaaagtcgc ttcttcgatt ttacggccgg taaaacgcag
      841 ggcgttcaat acggcggcgg cggtaatgat agccgtgccg tgctggtcgt cgtggaatac
      901 ggggattttg cagcgtttgc gtaattcgcg ttcgatgtag aaacactcgg gtgctttgat
      961 gtcttcgagg ttgatgccgc cgaaggtcgg ctctaaagcg gcgatgatgt ccacgagttt
     1021 ttgcgggtct ttttcatcga tttcgatgtc gaacacgtcc acaccggcga attttttgaa
     1081 caatacgcct ttgccttcca tcacgggttt gcccgccagc gcgccgatgt cgcccaagcc
     1141 caaaacggcc gtaccgttgg aaatgacagc gaccaagttg cctttggcgg tgtatttgta
     1201 ggcattttgc ggatcggcat ggatttccat acaaggagcg gctacgcccg gagagtacgc
     1261 caacgccaaa tctttgtcgg tcgccagaga tttggtcggg gtaacggaaa ttttgcccgg
     1321 cacggggaat tcgtggaact tgagggcggc ttctttcaat gagttttcca tgtgtcaatc
     1381 ctgttgcgag aaaaagattt gaatcggact gtccgaaacg ctttcgggtc atcatgttac
     1441 gggcggttcg ttaatattgc cgacgcatcc tgccttgtaa ccgagcgtaa ttctatcatg
     1501 ccgaaagttt ggcgcatacc gccattttca cctgcggcac tgccgtttga ttttcggcgc
     1561 gggcggatgc gcttgtttca atatttgccg catcagcagc cgaaatgtcc gtccaacact
     1621 ttttctatgc tgtttctgac ttcatccaag ttgcggttac tgtcgataac ggcgtaccgt
     1681 tcgggacagg cggcggctcg gtcgagataa acgccgcgca cacgcataaa gaaatccgcc
     1741 tgctcctgct cgaaacggtc tttctcgcgc gtctgcccga tacgcgccat cgacacttcg
     1801 agcggcacat ccagcagcag ggtcaaatcc ggcttcaaac cgccctgcac ccaatgttcc
     1861 aaaatttcaa tgtcttcaga cggcatcccc cgcccgccgc cctgataggc gaaggtcgcg
     1921 tcggtaaaac ggtcagacac gacgtgtatg ccgtctgaaa gcgcgggcag gatgacttcc
     1981 tcgatgtgct gcatacgcgc ggcgaacatc atcagggttt ccgcacgcaa accggctttg
     2041 gtttcagggt tgagcaggat ttcgcgcaag gcctcaccga ccggcgttcc gcccggctcg
     2101 cgcgtgaaca gcacgggcag ccccctccgt tcaaaccatg ccttgatgac ggcaaggttg
     2161 gtggatttgc cggcaccgtc tatgccgtcc aaagtgatga attgcggttt catgcttgca
     2221 gttccggtaa gggtatgccg tccgaaaaca caaacttttc agacggcatg gtttattttt
     2281 tcaaaatata tttgcggacg gcggcattgt gttcggtcaa atcatggctg aactggctca
     2341 agcccgtgcc gtccattttg gacacgaaat acaggtattt ttcgccggac ggatgggcgg
     2401 cggcatcgag tgccgccttg ccgggcagcg caatcggggt tggcggcaga ccgccgcgcg
     2461 tgtaggtgtt gtacggcgtg tcgcggcgca ggtcggcttt acggattttg cccttgtatg
     2521 ccgcacccat gccgtaaatc acggacgggt cggtttgcag gcgcataccg attttcaggc
     2581 ggttgacgaa gacggaagcg acatggtcgc ggtcggcttc atgccctgtt tccttttcga
     2641 ccaggctcgc cataatcagc atttcataag ggtttttata aggcagcccg tcctgcctgc
     2701 tttcccatgc ctcattcagg cggcgttgca tcgccttgta ggcggtttgg taaatctgca
     2761 aatcactgcc gcccgcatcg atttcgtagc tgtcggggaa aaactgccct tcaggattgc
     2821 cgctgaaggc atcgggcgca acttccgcca tcagtttttc attgctccag cctttggtgt
     2881 cgtgtccgat gtcgggcgtt gcgtcgatga ctttcctcat atgcgaaaaa cgcgaacctt
     2941 cgataatctg cacggtaacg gaatccggcc tgccgccgcg cattttctgc aagatatccc
     3001 aagcagacac ttccgaaggc aatctgtacg tccccgtatg cagcctgttg tgcacaccca
     3061 aaacgtaggc cgccgccgtc aaaacatgcc tgctgaacac gatgcggtct tcggcaagtt
     3121 tcctgccgac cgacgaaata ccctggtttt tggcaatttt gattcggtat gccctgccgt
     3181 tatccttagg aacaaaaagc agcgcggcga aaacggctgc cgacacggtc aaaaaaacgg
     3241 cagaccattt caacaatttt ctcaacatgg taggattccc agcattcgga atacgtctta
     3301 aaattcgggc gacagtataa cccaaattgc ccgccggaca ggaagaacat catggacaat
     3361 cacgccgaag cacactggca aaacggctgg cttcaaagca tacgccatac cccgtcgccc
     3421 aatttcagcc cgagggaaac gggggaaacg gtttccctga tcgtgttgca caacatttcg
     3481 ctgccgccgt tcgaatacgg cacggatgcg gtggaaaagc tgtttgccaa ccggctcgac
     3541 cccgacggac atccgttctt cagcctgata cacactttgc gcgtatccag ccatttctta
     3601 atcaaacgcg acggagaaac ggtacagttc gtttcctgcg acaatatggc ataccatgcc
     3661 ggcgtatctt cattcggcgg cagggaaaaa tgcaacgcat tttcaatcgg catcgaactg
     3721 gaaggctgcg atttcgaacc gtttgccgaa gcgcaatacc gttcgctcga agccttgttg
     3781 gacgcaatct gccgccacta ccccgtcacg gcggtaacgg ggcaccaaga catcgcgccc
     3841 ggccgcaaaa ccgaccccgg ccattttttc gactggcggc ggatacggga gaaagggttt
     3901 ctcgtggaca gaaatgccgt ctgaaaacca cagatttcag acggcatttc ggaaaatttc
     3961 cgcttatttc gacacactgc tgtcagaccc tgcaattttc gttataattc aaacgttata
     4021 atcggccgtt ccgtcagact gccggaaacg caagcccgat ttaaccgcag tgcaagcccg
     4081 atttaaccgc agtacccgca ctttgcgtat cctgcatccc gaaatttaac aagtgattgc
     4141 catgatttac atcgtactgt ttctagctgt cgtcctcgcc gttgtcgcct acaatatgta
     4201 tcaggaaaac caataccgca aaaaagtgcg cgaccagttc ggacactccg acaaagatgc
     4261 cctgctcaac agcaaaacca gccatgtccg cgacggcaaa ccgtccggcg ggtcagtcat
     4321 gatgccgaaa ccccaaccgg cggtcaaaaa aacggcaaaa ccccaagacc ccgccatgcg
     4381 caacctgcaa gaacaggatg ccgtctacat cgccaagcag aaacaggcaa aagcatcacc
     4441 gttcaaaacc gaaatcgaaa ccgccttgga agaaagcggc attatcggca actccgccca
     4501 caccgtttcc gaaccccaaa ccggacattc cgcaccgaaa cctgccgacg cgccggcaaa
     4561 acccgttccc gttccgcaaa cgccggcaaa accgctgatt acgctcaaag agctgtcgaa
     4621 ggtcgagctg ccctggtttg acgtgcgctt cgacttcatc tcctatatcg cgctgaccga
     4681 agccaaagaa ctgcacgcac tgccgcgcct ttccaaccgc tgccgctacc agattgtcgg
     4741 ctgcaccatg gacgaccatt tccagattgc cgaacccatc ccgggcatcc gctatcaggc
     4801 atttatcgtg ggtattcagg cagtcagccg caacggactt gcctcgcagg aagaactctc
     4861 cgcattcaac cgccaggtgg acgcattcgc acaaagcatg ggcggtcaga cgctgcacac
     4921 cgaccttgcc gcctttatcg aagtggcttc cgcactggac gcattctgcg cgcgcgtcga
     4981 ccagaccatc gccatccatt tggtttcccc gaccagcatc agcggcgtag aactgcgttc
     5041 cgccgtaacg ggcgtgggtt tcgttttgga agacgacggc gcgttccact ataccgacac
     5101 gtccggttcg accatgttct ccatctgctc gctcaacaac gagccgttta ccaacgccct
     5161 tttggacaac caatcctata aaggcttcag tatgctgctc gacatcccgc actctccggc
     5221 aggcgaaaaa accttcgacg atttgtttat ggatttggcg gtacgcctgt ccggccagtt
     5281 gaacctgaat ctggtcaacg acaaaatgga agaagtttcg acccaatggc tcaaagacgt
     5341 gcgcacttat gtattggctc gtcagtccga gatgctcaaa gtcggtatcg aaccgggcgg
     5401 caaaaccgca ttgcgcctgt tctcctaaaa tcgaatgcaa tcaaagcgga tatgtcgtta
     5461 cggcatatcc gctttttttc gggtaaaatt cagctgtttt caacctatca tttttcagac
     5521 gacctttgcc cgtttcatcc cgtatcagac aaaggtcgtc tgaaaacctt ctctcatgaa
     5581 tccaaactca aaacataaca ctaactttac caatcttctt aaaccgtctg atagtgatat
     5641 aaaacaattt gccgcacaac acatctgccg cctcaccgac ctcctcaacc gctacgccta
     5701 cgaatactac accctcgacg cgcccagcgt acccgatgcc gaatacgaca aattgttccg
     5761 cgaactcgaa gcgttggagt taaaccatcc cgagctcaaa ctgcccgaca gcccgaccca
     5821 gcgcgtcggc ggcgagcctt tggcgggatt tgccgaagta cgccacgaag tgccgatgct
     5881 gtcgctgacc aacgcttttt ccccgcaaga tgaaaacggc gcgttcgacc atgccgaaat
     5941 gtacgccttc gaccaacgcg tgcgcgacgg cttggacggc ggcaatcccg aatacgttat
     6001 cgaacccaaa ttcgacggcc tcgccatcag cctgctctac cgcgacggcg tattggtgca
     6061 ggcggcaacg cgcggcgacg gcacgacggg cgaagacgtt acccaaaaca tcaaaaccgt
     6121 atccaacatc cccttgcggc tgcacggcga aaatacgccc gaactcatcg aggtacgcgg
     6181 cgaagtgctg atgctcaaag ccgattttgt cgccctcaat aaaagacaag ccgaaaacgg
     6241 gcaaaaaccc tttgccaatc cgcgcaacgc cgccgccggc agcctacgcc aactcgattc
     6301 gcgcatcacc gcgcaacgca aactgcactt tttcccctac tccgtcgccc gccagcaaga
     6361 tggtttcgtc gcggaagaac acatccagga actcgcctat ttccaagcac tcggcttcag
     6421 tctgcccaat ggcaatttcg gctgtttcaa aaatatcgac gaagtattgg cgttttacga
     6481 acacatgcag caaaaacgcc ccgaactgcc ctacgaaatc gacggcatgg tggtcaaagt
     6541 caacagcttg gcgcaacaac gcgaactcgg cttcatctcc cgcgcgccgc gctgggcgat
     6601 tgcccacaag ttccctgccg aagaagccct gaccgtcgtc gaagcgattg acgtgcaaat
     6661 cggcaggacg ggtgcggtaa cgccggttgc ccgattacag cccgtattcg tcggtggcgt
     6721 aactgttacc aacgccaccc tgcacaatca ggacgaagta tcgcgcaaag acgttcgcgt
     6781 cggcgatacc gtcgtcgtac gccgtgccgg agacgtgatt cccgaagttg tgcgcgtgat
     6841 ttttgaacgc cgcccgatgc gggaaaccgc cgttgccgtt tcagacggca tcgggcatcg
     6901 gcaggacgat ttgtttgccg aaacaccgtc cgccaatcaa acccaatccg ttccgctcca
     6961 caagccctac cgcctgccga cccactgccc catctgccgc agcgaaatcg aacgcgaaga
     7021 aggcgaagcc gtcgcccgat gcagcggcgg tatgctttgt caggcacaac gcgcgcaagg
     7081 cttaatccac ttcgcctcgc gcaaagcgat ggacatcgac ggcttgggtg aaaaacaaat
     7141 cgaacagctt gtcgcgcaag acctcgtccg ccacttcgcc gatctctacc gcctcgacat
     7201 cccgaccttg caaaaaatga aggaaacagc ggacaaaacc gttgccgaat cggatcaaat
     7261 gccgtctgaa ggcagttcgg tcggcgcgtc gggcaaacat aaaaaacaac cagtcaagtg
     7321 ggcggaaaac atcctcgcag gcatagaagc cagcaaaacg cccgaactcg cccgcttcct
     7381 gttcgcgctc ggcatccgcc acgtcggcga acgcaccgcc aaaacgctgg cacaggcatt
     7441 cggcacattg gaacgcgtcc gccgcgcccc cgaacccgtc ctcgcctgcc tgcccgacat
     7501 cggtacggta gtggcgcgtt ccattgccca cttcttcgcc caagccgaac agcaggcgat
     7561 gatagacgaa ctgctcgccg caggcgttgc cccgcaaacc caagccgtta ccatcccgcc
     7621 cgcccgccac gccgaaccgc aacgctggat cgcccgcctg cccggtttca aaatcagcga
     7681 aaacaaagcc caagccttat gggaactcgc cggcaaaaac atagaagggc tgcaaaccga
     7741 caaagccctc cccaccgact ggcaggcgtg gcgcagcgaa ccacaaaacg ccgccctgct
     7801 cgaaaacctg aaaaccttct tcgcgcaaat gccgtctgaa gacgaggcgg cgcaaggttc
     7861 agacggcatc aataaagccg tggcgggcaa aaccttcgtg ttaaccggca ccctgcccac
     7921 cctcaaacgc gaccaagccc aatccctaat cgaagccgca ggcggcaaag tttccggcag
     7981 cgtgtccaaa aaaaccgatt acgtcgtcgc cggagaagcc gcgggcagca agctggaaaa
     8041 agccaatgcc ttgggcgttt ccgtcctcag cgaagccgaa ctgcttaccc tgctcggctg
     8101 aacccatgcc gtctgaaccg ccttcagacg gcattgccca cttttccgaa ccacaccatg
     8161 cacaccattt cctttccaaa ccgcacccga ctcaccgccc tgccgcccct gtcgctttac
     8221 atccacatcc cgtggtgcat caaaaaatgc ccgtattgcg acttcaattc ccacagcctg
     8281 aaaaacggat tgcccgaagc cgcctatatc gacgcgctgc tgaccgattt gcagcttgaa
     8341 ttgcccaata tttggggcag gccggtggaa acgatctttt tcggcggcgg tacgcccagc
     8401 ctgtttcagg cggaatcgat tgaccgtttg ttaagcggcg tgcgttcgct gttgcgcttg
     8461 cagccggaag cggaaattac cttggaagcc aatccgggga catttgaaat cgagaagttt
     8521 caaggattta aagacgcggg tattacgcgg ctttctatcg gcgtacagag tttcaacgac
     8581 gatatgctct caaggctggg gcgcgtccac aacggtaagg aagccttaac cgctatcgat
     8641 accgccttga aattatttga taaagtcaat atcgacttga tgtatgccct gccgaaccaa
     8701 accgttcaga cggcattgga cgacgtacaa accgctatcg ctacgggcgc aacccacatc
     8761 agcgcgtatc acctgacgat ggaaccgaac acgccgttcg gtcatacgcc gccgaaaggt
     8821 ttgccgcaag acgaagccgc cctcgacatc gaagacgcgg tacacggcgc gctggaaggc
     8881 gcgggttttg tccactacga aacatcggct tttgcgaaac cagccatgca gtgccgccac
     8941 aatttgaact actggcagtt cggcgattat ttaggcatag gcgcgggcgc gcacggcaaa
     9001 atttcctatc ccgaccgcat cgagcgcacc gtccgccgcc gccaccccaa cgactacctc
     9061 gccttaatgc aaagccaacc gagtgaagcc gtcgaacgca aaaccgtcgc tgccgaagat
     9121 ttgccgttcg aattcatgat gaacgccctg cgcctgaccg acggcgtacc cgccgcgatg
     9181 ttgcaggagc gcacgggcgt accgagtgcc aaaatcatgg cgcaaatcga aacggcaagg
     9241 caaaaaggcc tgctggaaac cgaccctgcc gtattccgcc cgaccgaaaa aggacgcttg
     9301 tttttaaacg atttgctgca gtg
//
LOCUS       DAWGRZ010000066         9174 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000066 DAWGRZ010000000
VERSION     DAWGRZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9174
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.066"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(38..763)
                     /locus_tag="WHT44_001124"
     CDS             complement(38..763)
                     /locus_tag="WHT44_001124"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ2533313.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSGDLDTGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGYAHSFECHYPDESGETRLAGGFYGVQIGSV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(833..1267)
                     /gene="fur"
                     /locus_tag="WHT44_001125"
     CDS             complement(833..1267)
                     /gene="fur"
                     /locus_tag="WHT44_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ2533314.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1486..1863
                     /locus_tag="WHT44_001126"
     CDS             1486..1863
                     /locus_tag="WHT44_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ2533315.1"
                     /translation="MNKTLILALSALLGLAACSAERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDVLQNAAEALKDRQNTDKP"
     gene            1873..2682
                     /gene="dapB"
                     /locus_tag="WHT44_001127"
     CDS             1873..2682
                     /gene="dapB"
                     /locus_tag="WHT44_001127"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ2533316.1"
                     /translation="MTPLKIAIAGVNGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIQAAGEKTGVVFAANFSVGVNLTFHLLDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKASSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2931..3341)
                     /locus_tag="WHT44_001128"
     CDS             complement(2931..3341)
                     /locus_tag="WHT44_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533317.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPTPNYIDWVKIKFKQFSYLKFIYGYATKSQDKDIDNILELGELKQDDEILDYGGT
                     LELIGGRCDLLTGFSIDIVCREIELEFLDQESFN"
     gene            complement(3343..3588)
                     /locus_tag="WHT44_001129"
     CDS             complement(3343..3588)
                     /locus_tag="WHT44_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533318.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNETELARMGLRL
                     KYNGPLKEEFKQLKFQKSSRTNLDSKNLKLIVFEKVK"
     gene            complement(3596..4750)
                     /gene="lpxB"
                     /locus_tag="WHT44_001130"
     CDS             complement(3596..4750)
                     /gene="lpxB"
                     /locus_tag="WHT44_001130"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ2533319.1"
                     /translation="MVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARFVGIGGE
                     LMKAEGFESLYDQERLAVRGFAEVVKRLPEILRIRRGLVRDLLSLKPDVFVGIDAPDF
                     NLGVAERLKRAGIPTLHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLYLDAGGR
                     AEFVGHPMAQLMPLEDDRETARKTLGVDAGIPVFALLPGSRVSEIDYMAPVFFQTALL
                     LLERYPAARFLLPAATAATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAADAVLVTS
                     GTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPELLQHDAV
                     PEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(4816..5808)
                     /locus_tag="WHT44_001131"
     CDS             complement(4816..5808)
                     /locus_tag="WHT44_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ2533320.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            6302..9061
                     /locus_tag="WHT44_001132"
     CDS             6302..9061
                     /locus_tag="WHT44_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ2533321.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAGRADEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETATVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
BASE COUNT         2298 a         2934 c         2171 g         1771 t
ORIGIN      
        1 ccaccatatt tgaacctgac cggagcataa gccgccatca aagccccttg cacgccactt
       61 cgcgccgccc gatttcctct ttcaacggca cggcgttcaa catccgcaga cgttcggcaa
      121 aatcagcaaa cggcagcagc tccgaaccga aacggcgcat atgttccgtg tcctgctggc
      181 agtctatcag ttccacgccc aaatccgcca aaaacggcac ggcgcaggca aacgcgattt
      241 tcgacgcatc cggttgcaac gcaaacatcg attcgccata aaacacgctg ccgatctgca
      301 cgccgtaaaa gccgcccgcc aacctcgttt caccgctttc atcgggataa tggcactcga
      361 aagaatgcgc gtaccccatt tcgtgcagct tcaaatatgc cgtctgaaac tcgggcgcaa
      421 tccaagttcc gtcctgattc gggcgcgccg ctgccgcaca atgcgcgacc acttccgcaa
      481 aacagccgtt gaccgcaacc cgatagctgc cgttgcgcag cgttttcgcc agcgagcgcg
      541 gaacgtgcag acgttcggga acaatcaccg cacgcggacc gaccgcatac caaaaaaacc
      601 aaccgtcccg agaaaaccac ggaaacacgc cgttccgata cgcctcaagc agccgccccg
      661 tatccaaatc gccgctcacg ccgaccagcc cgtcgcaccg ggccaaagca taggcaggat
      721 cgggaaaggc ataattgtca ggggcaagca gcggaatacg catggcagtt ctccaaaaac
      781 acagacggca tccaaaatca tgaatgccgt ctgaacaaca aaccgtccgg atttaacgtt
      841 tgcccttggc ctgacagtcg ctgcacacgc cgtacatata aagcgcgtga tcgacgatgc
      901 ggtagccgtt ttcttccgcg attttgtctt gcagggcttc gatttcggga ttgtggaatt
      961 ccgttacctc gccgcacttc acgcagacga tatggtcgtg gtggtcgccc ttgtccaact
     1021 cataaaccgc cttgcccgtt tcaaaatggt ggcgttgcag aatacccgcc tgctcaaact
     1081 gggtcagcac acggtaaatc gtcgccacac cgatttccac accctcttcc aacaaaatgc
     1141 ggtacacatc ttccgcactc aaatgctctt ccgcatgcgt ctcgaacaaa tccaaaatct
     1201 tcaaacgcgg gccggtaacc ttcagaccgc tgtctttcag ttgtgcaata ttgttgaatt
     1261 tttccataat attcaatacc cctgtaaaac aatagacgtt ataatacgca atttcggcct
     1321 gtttgcccac tatcgcacca tagcagtttg caatagtaaa accgcaccgg cggccgatgc
     1381 gcggacaggg cggataccgc ttaatcgtat gattatcgtt cgcttttata aaatattcaa
     1441 gcagtgttac actacccatc ccgatttgca cagaaaggca tttccgtgaa caaaaccctc
     1501 atcctcgccc tttccgccct cctcggcctt gccgcgtgca gtgccgaacg cgtttcactg
     1561 ttcccctcgt acaaactcaa aatcatacag ggcaacgaac tcgaaccgcg cgccgttgcc
     1621 gccctccgcc ccggcatgac caaagaccaa gtcctgctcc tgctcggcag ccccatactg
     1681 cgcgacgcat tccataccga ccgctgggac tataccttca acacctcccg caacggcatc
     1741 atcaaagaac gcagcaatct gaccgtctat tttgaaaacg gcgtactcgt ccgcaccgaa
     1801 ggcgacgtcc tgcaaaacgc tgccgaagcc ctcaaagacc gccaaaacac agacaaacca
     1861 taaggaacac acatgacacc gctcaaaatc gctatcgccg gcgtcaacgg ccgcatgggg
     1921 cgcgtattgg ttgaagccgt caacaaccat cccgacaccg tcctttccgg cgcgctcgaa
     1981 cactcgggct cagaagccct cgggctggac gcaggctacg ccgtcggact caaaaccggc
     2041 atcgccattt cagacgacgt tgacgccgtt ctcgcacaaa gcgacgtact catcgacttc
     2101 acccgccccg agccgaccct caaacacctg caaaaatgcg ttgaaaaaca agtcaacatc
     2161 atcatcggca caacaggctt cgacgatgcg ggcaaagccg ccatccaagc cgcaggcgaa
     2221 aaaacaggcg ttgtcttcgc cgccaacttc agcgtcggcg tcaacctcac cttccacctc
     2281 ctcgacaccg tcgcccgcgt attaaacgaa ggctacgaca tcgaaatcat tgaaggccac
     2341 caccgccaca aagtcgatgc ccccagcggc accgcgttac gcatgggtga agtcatcgcc
     2401 ggcgcgctcg gccgcgacct caaacaatgc gccgtttacg gccgtgaagg ccacaccggt
     2461 ccgcgcgatc cgtccaccat cggctttgcc accgtccgcg caggcgacat cgtcggcgac
     2521 cacaccgccc tcttcgccac cgacggcgaa cgcgtggaaa tcacccacaa agccagcagc
     2581 cgcatgacct ttgccgccgg tgccgtccgc gccgcagttt gggtaaacgg caaaacgggt
     2641 ttgtacgata tgcaggatgt actcgggctg aacaaccgtt aacccccata caaaataccg
     2701 tctgaaaaga tattgttcag acggcatttt gccgacaggc tccgtatcgg catatcaatg
     2761 tttcaacaca caggacgaca cataaagcgt cgccctatgt gttgccctga ttcggaaggg
     2821 gttacgcccc tcccaaataa agtctgattc taccgcccta aagggcgggg tttcaaccga
     2881 aaaggaaata cgataaagtt gcctgcgcag cataacaagt tacggctcgt ttaattgaaa
     2941 ctctcctgat ctaaaaattc taactctatt tcccggcaaa ctatatctat actaaaacca
     3001 gttagaagat cacatctacc gcctatcaat tccagcgtac ctccataatc caagatttca
     3061 tcatcctgct ttaattcgcc aagctccaat atattatcga tatctttatc ttggctcttt
     3121 gtagcgtatc catagataaa tttcagatag ctgaactgct tgaatttaat ttttacccag
     3181 tctatataat ttggtgtagg aatctcttga ggaaagtccg gataatccac attctggacg
     3241 atgatatgga attcatcgtt tttcaaagta tagtttaata gagtgccctc atatatatac
     3301 agaaattgat aatctttttt tggaatggta taagttgtca tattatttta ccttttcaaa
     3361 aacaatgagt ttaagatttt ttgagtccaa gttggttcta ctactcttct gaaatttcag
     3421 ttgcttaaac tcttccttta acggcccatt atattttaac cttaatccca ttcgggccag
     3481 ttctgtttca tttggaattc ctcttaaata cttattgttt gatgtgccat taatcacaat
     3541 ttctccccct ttttttgtaa tacgtgctgc ttcgggaaaa taatccatta accgctcaac
     3601 atcccgcctc ttccaaaacc gcgcgcgcgg ccaaatccgc cgtatctttt ttcaacagca
     3661 ggtgcaacgc cctgaaatcc tgttgcagcg cggcaacctt atcggggtgt tcgtaccagt
     3721 ccgccaacgc cgccgccagt ttttccggta cggcatcatg ttgaagcagc tccggcacag
     3781 cctccttacc caacaggata ttcggcaggc cgacatgcgg cactttgatt ttgcgtttca
     3841 cataagcata agtcagcggc gaaatcttgt agctgatgac catcggacgc ttgcacagag
     3901 ccacttccaa agttgccgta ccgctcgtta ccagcaccgc atccgccgcc ctgcacactg
     3961 tttcagactg tctgtcgatt accgtcagcg gcaatccggc aaactccggc cgctgcaaaa
     4021 cttccgccaa acgccgcttc gtcgccgccg ttgcggcagg cagcaggaag cgtgcggcag
     4081 gatagcgttc caacaacaat aatgccgtct gaaaaaacac cggcgccata tagtcgattt
     4141 cgctgacgcg gctgccgggc agcagggcga atacggggat gccggcatcc acgcccaaag
     4201 ttttccgcgc cgtttcacgg tcgtcttcca agggcataag ctgcgccatc ggatgaccga
     4261 caaactccgc acgtccgccc gcatcgagat aaagctgcgg ctccatcggg aacaggcaca
     4321 gcacgcggtt gacctgatgc acgattttgc ccacgcgttc ccgccgccac gcccacaccg
     4381 acggactgac ataatgcagg gtcggaatgc ccgcccgttt gagccgctcc gccacaccta
     4441 gattaaaatc gggcgcatcg atgccgacaa acacatcagg tttcaacgac agcaaatccc
     4501 gtaccagccc cctgcgtatc cgtaaaattt ccggcaggcg ttttaccacc tcggcaaagc
     4561 cgcgcaccgc cagtcgctcc tgatcataaa ggctctcgaa accttccgcc ttcatcagtt
     4621 cgccgccgat accgacaaaa cgcgcctgcg ggcaacgctt tcggatggcg cgtatcaggt
     4681 gcgcccccaa caggtcgccc gacgcttcgc cgacactgac ggcaatcaaa gggctttttt
     4741 tatcaaccat attcgttctt cttcacatac tttcacgtcc cgatgccgtc tgaaggcttc
     4801 agacggcatc ggggttcaca ttttcgtccc gttttccaac atcaccgcaa actgcgccaa
     4861 gtccggcggc agctccgcct tcaacaccag cggctcgccc gtgagcggat ggttcaagtg
     4921 cagctcggac gcgtgcaaaa acatccgctt caaacccaac ttctgcaaac gacggttcgc
     4981 ctgataatcg ccgtagcgtt cgtcgcccgc aatcggacag ccttgagatt gcaggtggac
     5041 gcggatttgg tgcgtgcgcc ccgttttcaa cgtcgcccgc accaaagtca ggtgcgacag
     5101 cccgacaccg tgcaaaatgc cgtctgaaaa acggcttaac acacggaaca ccgtatgcgc
     5161 cgactgcccg tccgcactga cgcgcaccat cttttcgcct tgtgcgccgg tatatttgaa
     5221 caggggcagt ttgacatgga aattgtcgtc cggcagtttg cccaccccca gcgcaaggta
     5281 gatttttttg gggtggtcgt tacggatggc ttcgtgaagt ttgacgagcg cgctgcgttt
     5341 cttcgccacc atcaacaagc cgctcgtatc cttgtccaaa cgatgaacca actccaaata
     5401 cttcgcctcc ggacgggcgc ggcgcaactg ttcgataacg ccgaaactca cgccgctgcc
     5461 gccgtggacg gcaacgccgg acggtttgtc gatgaccaaa agcgcatcgt cttcgtaaac
     5521 aacgtcaaac gcacgcgccg gtacggcggc acgcctttca gacggcattt ccttctccgc
     5581 cacgcgcaca ggcggaatcc gcaccgtatc cccctccgca atacggctgt cgggtttgca
     5641 gcgtttcttg ttcaaccgca cctcgccggc gcggataatg cggtggatat ggctcttggg
     5701 aacacccttg aggattttta tcagatagtt atcaaggcgt tgacccgcct catgttcggc
     5761 aacccctatc aagctgaccg aatctttgct tatttcgtgc gttttcatct ataatccgaa
     5821 catccgtttc agcaaaaagc gcgcccgccg cgcccccctg aaacggttta ctttaaaacg
     5881 gaattttata ttaaaaacgc actccgcaaa gcattttccc tgcgcctgtt cccagccggc
     5941 aggcgcagaa cgtaatcaag tttgaattga ttttgccgtt tcggcgcgga agtaagacgg
     6001 cagccgggcc ccaagtccca aacgcaaacc gcccaaaaca caggcatcac gataacaaga
     6061 agaagccggc ctccattttt ccgaccgcag cgttcggaac gcggcagaca aaccgccgca
     6121 tcgatcttta tagccttctg tatccgaccc ttccgcacac ggttctccgg aacgtgcatc
     6181 cctgcggatt ttcaactcaa aaactgatta ccggttttat ccgaaaacat cggaaaaccc
     6241 agccttacgg aatgccgaac cggacaggtg cgcccgaatg ccgcccgtcc acgaggtgat
     6301 catgaaaaga atgttattca acgcaacgca ggccgaagag ctgcgcgttg ccatcgtcga
     6361 cggacaaaac ctgctggatt tggatatcga aacgctgggc aaagaacagc gcaaaggcaa
     6421 tatctacaaa ggcatcatta cccgcatcga gccgtcgctg gaagcgtgtt tcgtcgatta
     6481 cggaaccgac cgccacggct tcttgccgtt taaagaagtc tcccgctcat acttccaaga
     6541 ctacgaaggc ggacgcgcgc gcatccaaga cgtgctcaaa gaaggcatgg aagtcatcgt
     6601 ccaagtcgaa aaagacgagc gcggcaacaa aggcgcggcg ctgaccacct tcatcagcct
     6661 cgccggacgc tatctggtat tgatgccgaa caacccgcgc ggcggcggcg tatcccgccg
     6721 tatcgaaggc gaagaacgtc aagaactcaa agccgctatg gcgcaactcg acattccgaa
     6781 cggcatgagc atcatcgccc gtaccgccgg catcggccgc agcgcggaag agttggaatg
     6841 ggatttgaac tatctcaaac aactctggca ggcgattgaa gaagcaggaa aagcgcatca
     6901 cgacccttac ctgctcttta tggaaagctc gctgctcatc cgcgccatcc gcgactattt
     6961 ccgccccgac atcggcgaaa ttctggtgga caatcaagaa gtttacgacc aagttgccga
     7021 gttcatgagc tacgtcatgc cgggcaatat aggccgtctg aaactctacg aagaccacac
     7081 gccgctgttt tcccgcttcc aaatcgaaca ccaaatcgaa agcgcgtttt cgcgcagcgt
     7141 cagcctgccc tccggcggcg cgatcgtcat cgaccatacc gaagccctcg tctccatcga
     7201 cgtgaactcc gcacgcgcca ctcgcggcgc agacattgaa gacaccgcgt tcaaaaccaa
     7261 tatggaagcc gccgaagaag tcgcccgaca aatgcgcctg cgcgacttgg gcggcttggt
     7321 cgtcatcgac ttcatcgaca tggaaaaccc caaacaccag cgcgatgtgg aaaacgtcct
     7381 gcgcgacgcg ctcaaaaaag accgcgcccg cgtacagatg ggcaaactct cccgtttcgg
     7441 acttttagaa ttgagccgcc aacgtttgaa accggctttg ggcgaaagca gccacgtcgc
     7501 ctgtccgcgc tgcgccggca ccggcgtgat tcggggcatc gaatccaccg ccctgcacgt
     7561 tttacgcatc attcaagaag aagcgatgaa ggacaacacc ggagaagtgc gcgcacaagt
     7621 gcccgtcgat gtcgccacct tcctgctgaa cgaaaaacgc gccgagctgt ttgcgatgga
     7681 agagcgtttg gatgtcaacg tcgtcctgat tccgaacatc cacctcgaaa atccgcacta
     7741 cgaaatcaac cgcatccgca ccgacgacgt agaagaagac ggcgaaccga gctacaaacg
     7801 cgtcgccgag cctgaagaag acgaatccgc caaaccgttc ggcggcgaaa aagccaaagc
     7861 cgcccgtccc gaacccgccg tcaaaggcgt gcgccacacc agccccgccc cgactgccgc
     7921 ccccgagaaa aaaacctcct ggtgggacag cttcaaagct tggctcaaac gcattttcgg
     7981 cggcagcgaa acccaagccg cgcccgctgc cgaaacctcc gaaaaacgca gcacggcaaa
     8041 ccgcagcggc agccgcgcca acaaccgccg ccaaaacccg cgccgcagca aacgcgaagg
     8101 cagcaaagta gaagtccgcg aagtggcagg caaaactgcc ggacaggaag cgcgtgccga
     8161 caaagccgaa acgcgcaaca acggcaaccg ccgccgcaac gaacgcggcg accgtgccgc
     8221 cggacgcgcc gacgaagcgg aaatccaaga ccgcaacata cagcctgccg caaccgttgc
     8281 agatgccgca ccgtccgaaa ccgaagtgca aaccggaaaa cgccgccgca acggcagccg
     8341 cagcgaacgc ggccaaaccg cgccggaaac cgccaccgtt gccgaaacaa ccgttcagac
     8401 agcggaaaac acgccgtccg aaccgcatac cgcagaagac aaaggcagca agcccaaatc
     8461 cgaacgcaac cgccgcgaac gcgacagccg agatgccaaa gaacgccgcg agcgcaacaa
     8521 tcagcgcgac cgccgtcaaa acggcaaaaa acgcaatatt ccgtctgccg ccaaaatcga
     8581 gcagtacctg aatattcacg acaccgccga caaagtccgt tccgccgccg cgcacgtttt
     8641 cggcgaaacc gacgcaaacg cgccgattac tgtcagcatt gccgatccgg ttgcagaaag
     8701 ggatcttccg acagcatctc ccgccgtttc aaacggcgac gcaccggttt atgatgcggc
     8761 ggaaaaaatc cgccgtgcca ccgccgccat cctgcccgaa ggcgcgacac cgaaagccga
     8821 agcacaggaa atgccgtctg aaaccgcaac ctttacggct gcggcggaac aggcacggga
     8881 aaccgcacaa accggcggac tcgtcctgat cgaaaccgac cctgccgcat tgaaggcatg
     8941 ggcggcacaa cccgaagtcc aagccggacg cggtttgcgc cgttccgaac agcccaagcc
     9001 gtctgaagtc gcaaccgtcc ctgccgaaga aatgatccaa gtcgaaaccc ggcaaggctg
     9061 aaccgacggc ggcaaaaaga ggttctgttc cgcagaacct cttttttaca tgggttcgga
     9121 tacctgcaat gccgtctgaa acttcgccat tcccgtgatt accgaaacat tccg
//
LOCUS       DAWGRZ010000067         9106 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000067 DAWGRZ010000000
VERSION     DAWGRZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9106
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.067"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(44..3088)
                     /locus_tag="WHT44_001133"
     CDS             complement(44..3088)
                     /locus_tag="WHT44_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEZ2533322.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSHMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFHRHAGQGKGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEA
                     RISRLIPESQTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDETADIHIEEPAAPDA
                     WVVEPPEVPKVPMTAIDIQPPPPVSEIYNRTYEPPSGFEQVQRSRIAETDHLADDVLN
                     GGWQEETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAVCPFENVPSERPSCRVSDT
                     EADEGAFPSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLLPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3253..3933
                     /locus_tag="WHT44_001134"
     CDS             3253..3933
                     /locus_tag="WHT44_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533323.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMMGTSAALWLAALLMLPYCLLFA
                     GSVGAYPPNALVLILPALTVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3962..4321
                     /locus_tag="WHT44_001135"
     CDS             3962..4321
                     /locus_tag="WHT44_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ2533324.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPASIPPATGNLFANWIGA
                     FLIGIFAETVNHPQWKLLLITGFLGSLTTLSGFSLETVTLLQLNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
     gene            complement(4426..4656)
                     /locus_tag="WHT44_001136"
     CDS             complement(4426..4656)
                     /locus_tag="WHT44_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533325.1"
                     /translation="MNIFVTNFCYWFISLLAISYIPSINAWHDELIDDINFGKRVMMV
                     TFFAFLGTVIERFFKKKPWWFYPAKAFSLLQT"
     gene            complement(4835..5371)
                     /locus_tag="WHT44_001137"
     CDS             complement(4835..5371)
                     /locus_tag="WHT44_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ2533326.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDEGKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYTAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(5405..5761)
                     /gene="folB"
                     /locus_tag="WHT44_001138"
     CDS             complement(5405..5761)
                     /gene="folB"
                     /locus_tag="WHT44_001138"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ2533327.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            5817..6419
                     /gene="plsY"
                     /locus_tag="WHT44_001139"
     CDS             5817..6419
                     /gene="plsY"
                     /locus_tag="WHT44_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ2533328.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPVAASFF
                     MPHVSWVWATVAIALLVLFRHKSNIVKLLEGRESKIGGSR"
     gene            6496..7341
                     /locus_tag="WHT44_001140"
     CDS             6496..7341
                     /locus_tag="WHT44_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ2533329.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPKLYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGSRYLL
                     DSKIR"
     gene            7587..8561
                     /locus_tag="WHT44_001141"
     CDS             7587..8561
                     /locus_tag="WHT44_001141"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ2533330.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANSAVAGLASEEEDTFVNAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKENPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPAKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVFMGSSEEVAYLDRLHAK"
     gene            complement(8704..8922)
                     /locus_tag="WHT44_001142"
     CDS             complement(8704..8922)
                     /locus_tag="WHT44_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ2533331.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         2096 a         2701 c         2111 g         2198 t
ORIGIN      
        1 ccgtctgaaa cagtcttcag acggcatttc catttgcaaa aaatcaagca ttgtccaagg
       61 ggacgagaat cgtacggttg ccgttgtgtt ccggtgcgga cacaatgcct tccgcctcca
      121 tctggtcaat cagacgcgcg gcgcggttgt agccgatacg caaggcgcgc tgtacgcccg
      181 aaatgctggc tttgcgcgtt ttcaggacaa cggatacggc ctcgtcgtac atcggatcgg
      241 tttcgtcgtc gccgctgcgc ccgatgccgg gcagctcttc gctgccgccg ccgctcaaaa
      301 tatcgtcaac atagtccggt tcgccaaact gtttcaaata ttcgaccacg cggtgcacct
      361 cttcatccga ggcaaacgcg ccgtgaacgc gctgcggata ggcagtaccc ggcagcagga
      421 acagcatatc gccctgaccg agcaggtttt ccgcgcccat ttggtcgaga atcgtgcggc
      481 tgtcgatttt gctggacact tggaacgcga tacgcgtcgg gatgttcgcc ttaatcagac
      541 ccgtgatgac atcgacgctg gggcgttgtg tggcaagaat caaatggatg cctgccgcgc
      601 gggctttttg ggcgaggcgg gcaatcagtt cttcgatttt cttgcctgcc gtcatcatca
      661 ggtcggcaaa ctcatcgacc acgaccacga taaacggcag tttttccaaa ggttcgggat
      721 cgtcgggcgt gaggctgaac ggattgccga ttttttctcc ccttgctgcg gcttcggcga
      781 ttttttgatt gaagcccgca agattacgca cgcccataaa gctcatcagg cggtagcgtt
      841 tttccatttc gttaacacac cagttcagcg cgtttgccgc cagcttcata tcggtaacga
      901 caggggcgag caggtgcggg atgccttcgt aaatgctcaa ttccagcatt ttcggatcga
      961 tcataatcat acgcacgtct tccggcgcgg ctttgaaaag catagacaga atcatcgcgt
     1021 tgacacccac cgatttgccc gaaccggtcg tgccggcaac caacaaatgc ggtgcttttc
     1081 ccaagtcggt tacgacgggc tgtccggtga tgtcctgacc gagcgcgagc gtcagcttgg
     1141 atttggattc ggcaaactcg ggcgaattga agatttcgct caggcgtatc atttggcgtt
     1201 tcgggttcgg aagttccaaa cccatgcagg ttttgccggg gatggtttcg acaacgcgga
     1261 tggaagccac gccgagcgaa cgcgccaaat ctttttccag attcagaacg gaattgccgc
     1321 gcacgccgac atcgggttcg atttcataac gcgtaattac ggggccggaa taagaatcga
     1381 caaccttgac cttgactttg aactccgcca atttttcttc gatggtgatg ctgttttcca
     1441 acagttcttc ttcggtttgc gtcgcctcgg gattgaacag cggaggcaga agcaggtcgg
     1501 ttgtcggcag gtgttcggat accgcaccgg tttcttcaga tgggaacgcc ccttcatccg
     1561 cttccgtatc cgatacccgg caggacgggc gttcagacgg cacattttca aacggacaaa
     1621 ccgcctgact gtcatgcccg aacgcttcgg tttccgacag atattgcccg cttgaccgct
     1681 ctgccgcacc ttcactgccg tcatccgcaa tagcggcggt ttcctcctgc caacctccat
     1741 tcaaaacatc atcggcaaga tggtcggtct cggcaatgcg gctgcgttgc acctgctcga
     1801 atcctgacgg cggttcatag gtacggttgt agatttccga tacgggaggc ggcggctgaa
     1861 tatcgattgc ggtcatggga actttcggca cttcgggtgg ttcgaccacc caagcatcgg
     1921 gcgcggcagg ttcttcaata tggatatcgg cagtttcatc ataaaccgga ccgccgtatc
     1981 ccacagacga aacggtttcc gtgaaaacat tttcggtttc agacggcatt tcgacatccc
     2041 gtttcccgac aaccgtctga ctttccggaa tcaggcgaga aattcgcgcc tccgcagaaa
     2101 ccgttgcctt gttcggttct ttcaaattga cggaaacgcg gcggcgggca tcgcggacgg
     2161 ccgtgccgtc tgaaacggac tgcccttggg aaacatccgg ggattttgcc tccgcctgcc
     2221 ctttcccctg ccctgcatga cggtggaacg gcgcattccg tgcattttcg gctttgactg
     2281 cagaaaaggc agacggatgc tgcttctcaa aatacggctc aaatccgtaa tccgcagact
     2341 cggaaaacgc ttctttgtcc gcatcgaaca tatgggaata cgtgcgttga agcacagggt
     2401 cgtccaaacc gataatctgc agcccttcca tcggggtatc ggacacgcgg actttgggga
     2461 ccgctgtttc gtttttctca aatgcatcga tatagcgttt tttcgtttcc cttaaagccg
     2521 cgctgtttaa agcacgcgtt gcttcttcca aagtgatttc tttaaaaacc ggacggacgg
     2581 gcgaaatttc gctttcagac ggcatcaacc cttcagcaat actccggtcg aatgggatgc
     2641 ggcggttgtc gattacggca gttgcaatgt cttccgtatc ggcagcctct tcctccgcag
     2701 cttctgcttc ttccgtttcc gcttcttccg tcccgtttcc actgtctgaa tatccgtcgg
     2761 atgcggtcgg aacatccgca cggtttgcag aagcaggcgg aacgggcctg tccggttcgg
     2821 attcgtgcgc cagatagttt cggcagaaac ggacgacacc gacaaacagc caatacactg
     2881 ccgttttgac ggcatggaaa agcatcaggg caagttcggg aaaatcgggc ataccgtctt
     2941 tgatttcagg cagctccgcc tgtttttccc ctttcttttc ctgccacgca gaaacctcgc
     3001 gcatccactc gcgttcggat tgtgcgcgga caaaaaacaa gccggcaagc gcaagcaaca
     3061 aaataacgat caaaactatc caaaacatat gccgtttccc gcaagacaaa cgattaaacg
     3121 ggtttatttt aacgtgtttc catcccaaaa aaaacaaccg gccggcaatt ctgccgaaac
     3181 cgattcggct ttcggtttgc ccccgccgac agtataatag ccgtcccttc ccttaatctt
     3241 tgcggcattg ccatgatttt ccaaacagtc tggttttcag atatggtact gtcggtttcg
     3301 tggattgtcc ttatcctgat actggcagct tccgcgccgt cggcattccg ctcgcttgcc
     3361 cgctaccgtt ccgcccttcc cctgtgtacc gtaatttttt ccgcagcatg gtgtctgaac
     3421 gcctctgccg gcggcggaca gcttgcccag atgaactacc acctgctcgc cgtcaatttg
     3481 gttgcactga tgatgggcac ttccgccgcc ctttggcttg ccgcgctgct gatgctgccc
     3541 tactgcctgc tgtttgccgg ttctgtcggc gcatatccgc cgaacgcttt ggtgctgata
     3601 ctgcccgcgc tgaccgtcaa ccgcctgtcg cgtatgctgg tcaaccgcct gccgcccaat
     3661 attttcatct ttattttcgt caacggcttt ctcgcttccg ccgccggcat tctgctgacc
     3721 gggctggtgc tgaccggtat tttggatgcc gcaaacgctt ttccgtccga aatattgtgg
     3781 acgaccgccc tgcccgtctt tattctgctg gcgtgggcgg aagccttcct cagcggcatt
     3841 tcaactgcca tatttgttgc actgaagccc cattggatta acactttcga cgacaaccgc
     3901 tatcttaagt ccgaccgcgg catatggcgg tagccttcag acggcataga caggcaaaac
     3961 catgctttcc aacatcatcc ccctttccat cggtgcggca ctcggtgcaa cggcgcgttg
     4021 gctgctcaac ctcgccgttc ccgcatcaat acctcccgcc acaggcaacc tgtttgccaa
     4081 ctggataggc gcttttctta tcggaatctt tgccgaaacc gtcaaccacc cgcaatggaa
     4141 gctgctgctg attaccggtt ttctcggcag cctgaccacg ctttccggat tttcgctgga
     4201 aaccgtaacc ctgctccaat tgaaccgtcc cgcttcagca cttgccaata ttttcctgca
     4261 cacggcaggt tcgctgctgc tgacctggct cgggctgaag ataggggcgg cagtcaaata
     4321 accgcaacaa attgcctccc tttgttttga cataatctat aaattagctt cgcttgttta
     4381 aatactggct gtgccaatta ccgttctcaa attcaaattg agcttttagg tctgtaacaa
     4441 agaaaaagcc ttggcaggat aaaaccacca aggctttttc ttaaaaaaac gctctattac
     4501 cgtgcctaaa aatgcaaaaa aagtaaccat cataaccctt ttgccaaaat taatatcatc
     4561 tattaattca tcatgccatg cattaataga tggtatataa gaaatagcca ataaactaat
     4621 aaaccaataa caaaaattag taacaaagat attcattatt aaccatttta aaaattatca
     4681 cgttctttta ggtaattatc atcccctttt ctctcttatt actaaagcac gaatgacttt
     4741 aaaggacgcg cccagataca agccgaaaag gcatatccca tccgccaaac aaaccgcccg
     4801 ccgatccaat ccggcgggca agtccaacat cctgtcaatc cttcatcaac cagtattgca
     4861 aaccgattaa tgtcttgccg tctttaattt catcgtttgc caatgcctga cggacttctt
     4921 ctttcgacat caatacggtt tccgtaatct cgtcttcgtc attggcaagc gtactgccca
     4981 aacgcacgcc ttccgcttcg aacagataca ttttttcgtt gcaaaaaccg accgccgtat
     5041 aaaaactgta aagcaggcgt acgctgtcgg cggtataagg tgtttcctcc gccaattctc
     5101 gcagcgcaca cgctgccata tcctcgcccg ccacatccag cttgcccgca ggaagttcca
     5161 atgtcgcctg atttgccgca taacgccact gccgcaccaa aaccactttc ccctcgtccg
     5221 taaccgccaa cacgcatgcc gcacccggat ggcggatgac gatacgctgc ccttcattgc
     5281 cgttgggcaa cctgacctta tccctgctga tactgacgaa accgccctcg taaatggttt
     5341 cgccgcctaa ttttacttcc ctcaaatcca cttcatatct cctttccgtt tcctattctg
     5401 ccgttcaatc ttcacgcttg ccgcgctcga tttccacgcc aacctcgcgc acgccttcca
     5461 aaatacccgg cttgacgatt ttcacgcgca cccacaccgc gccgaaatat cccaaaacca
     5521 aatcggcaat atattccgcc aacgcttcca aaagcaggaa atcctgttct ttcagatgtc
     5581 ggcgcagcgt ttcgcatacc tcggcataat gcaccgtatt ggcaatatcg tcgtccgaac
     5641 ccgctttctc gggaacaccg atgtccaaat cgacaatcag ggtctgcaac cgttcgcgtt
     5701 cccagccgta cacgccgata agcgtatctg ccttcatgcc gtgcaaaaag attttatcca
     5761 taatccgccc cgttttttgc taaaataacc gcaccattct aagtaaagta ccgcaaatgt
     5821 tcaacatacc ggctgttgct gtttcctatc tgatcggctc actttctttc gccgtcatcg
     5881 tgtccaagta ttacggcatg gacgacccgc gcacctacgg atcgggcaat ccgggggcaa
     5941 ccaatgtttt acgcagcggc aaaaaaaagg cggccgcgct gacgctcttg ggcgatgccg
     6001 ccaaaggttt agttgccgtt ttgcttgcac gcgtgcttca agaaccgctc ggtttatccg
     6061 acagcgcaat cgcggccgtc gcactcgccg cgctggtcgg gcatatgtgg ccggtgtttt
     6121 tcggatttaa aggcggcaaa ggcgtggcaa cggcattggg cgtgcttctg gcactctctc
     6181 ccgcaactgc cttggtctgc gcgttgattt ggcttgttat ggcattcggc ttcaaggtgt
     6241 cctcccttgc cgcattaacc gccacaatcg ccgcaccggt cgccgcatcc ttctttatgc
     6301 cgcacgtctc gtgggtttgg gcgaccgtcg ccattgcttt gctggtgttg ttccgccaca
     6361 aaagtaatat cgtcaagctg ctcgaaggca gagaaagcaa aatcggcggc agccgctgat
     6421 ttcccatcca tccctgttta tgccgtctga accccgcttc agacggcatc cgaatatccc
     6481 gccgttttac cgactatgct cagttacaga cacgcattcc acgccggcaa ccacgccgat
     6541 atgctcaaac atttcacctt gtttttagtg ctgcaatatt tcaaccgcaa agacaagccc
     6601 tactggtaca tcgacacgca cggcggcgcg ggtgtgtaca atctcgaagg cagcgaggcg
     6661 cagaaagtcg gcgaataccg acaaggcatc gccctgctcc gacaggcgca aaacctgcct
     6721 gccgaactct ctgactttgc cgcgcacata caaaaaatcc tgccctcgcc caaactttac
     6781 tgcggttctc cgtggctggc gcaatcgctg acccgcgtcg gcgacaaatt acgcctgttt
     6841 gagctgcacc ccaccgactt tgtccatctg caaaacaata tgggcgaagc aggattgggc
     6901 aaacgcggac aagtattacg cgaagacggt tacaaagggc tgatttccct gctgccgccc
     6961 ccgccgcgcc gcgccaccgt cctcatcgac ccgccctatg aggaaaaaca ggactaccgg
     7021 cgtgtaacgg aaacgctgaa agccgcttta aaacggtttg agtccggctg ttatctcatt
     7081 tggtatccct gcctcagccg cgaagaaagc cgcaagctgc ccgaagaatt gaaaaaactc
     7141 ctgcccgaca actaccttca cgccgaactt cacgtccacg cgccgaaagc cgacggtttc
     7201 ggtatgcacg gcagcggaat gttcgtcatc aatccgccct atctgttggc ggaacaattg
     7261 gcggcaaacc tccccgccct gacgcggctg ctggcgcaag acgaaggctc gcgctacctt
     7321 ttggacagca aaatccgctg accccgatgc cgtctgaaac ccttcagaca acatctcccg
     7381 tcaaataaac aaaaccggac gatataccgg cagtcggcac ttttaagaat aaatatcccc
     7441 tcatccgcct gccgcgttcc gacttgcccc atgccgtttt tttacactac aatcccctag
     7501 actatcctgc aaaacgcacc aaaggaatac gccacaataa cctgccgcct ttcagacggc
     7561 acaaaccttt ccgaaacgag caaactatgg acacactgac ccgattcctc cccgaacacc
     7621 tgcaacaaaa ccagctgccc gaagcactcg gcggcgtttt actgtccgtt gtttccgcct
     7681 gcaccgaaat caacgccaaa gtccgcctcg gcgcactggc cggcgtattg ggtatggcgg
     7741 gcacgggcaa tattcagggc gaagaccaaa aaaaactgga tgttatcgcc aacaacatca
     7801 tgattgacac actcaaagcc aattctgccg ttgccgggct ggcgagcgag gaagaagaca
     7861 ctttcgtaaa tgccggtgaa aacggacgct atctcgtcct attcgaccct ttagacggat
     7921 cgtccaatat tgatgtcaac atttccgtcg gtacgatttt ctccatcctt gaaaaacccg
     7981 aaggcgcatt ggcaaccgaa tcattccttc aaacgggcag acagcagctt gccgctggtt
     8041 acgttcttta cgggcctcaa acccagctcg tattcacatt cggacatggc gtatatatgt
     8101 tcacgctcaa tgccgaaaac gaatttgtgc tgaccaaaga aaacccgaaa gtacccgaaa
     8161 gtaccaaaga atttgccatc aatatgtcca accgccgcca ctggctgccc cccgtccaac
     8221 aatacatcga cgagctcttg gcgggcgaaa ccggtacgcg cggcaaaaac tacaatatgc
     8281 gctgggtagc cagtatggtt gccgaaatcc accgcatcct gatgcgcggc ggcgtgttca
     8341 tgtatccgca agacaaacgc gaccccgcta aacccggcaa actgcgcctg atgtacgaag
     8401 ccaaccctat gagcctgata ctcgaacagg cgggcgcatc ggcaagcaac gcatatcaag
     8461 ccatgctcga catacagccc gaaaacctgc accaacgcgt cgccgtcttc atgggcagca
     8521 gcgaagaagt cgcctatctc gaccgcctgc acgcaaaata gacagacagc cgttcacaca
     8581 agtacacggc ggcggcaatc cgataccgcc gccgttattt caaacagcac aagccttccg
     8641 gtcaaatgac aaaaatgccg tctgaacaca tttcagacgg catttcaaaa actcaaaccc
     8701 gaattatttt cccgctgctt tttgcaggat ggcgtacaac tcgtctttca gtttcaattt
     8761 ggcttttttc agctcatcga tggtttccgc accgctggta accggattgt tgaccagacc
     8821 ggtaatttta tcgtccagct cgttgtgttc gtcgaacaga cgggcgaagc gggaattttc
     8881 ctgtttcaat ttggaaatca aatcacggta ttctgggaac atatcgcttt cctctcttgg
     8941 tttacggtta aaaagtcaaa ttgaatcttt acattaatta taacaaagat aatattttta
     9001 tccagtgttt tatcggatac ggcaaaattt tcttttaaca ccgccgtccg gttgataaaa
     9061 acgttgacaa tatcacgctt tacgcataaa ttttcagcct ttccta
//
LOCUS       DAWGRZ010000068         9094 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000068 DAWGRZ010000000
VERSION     DAWGRZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9094
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.068"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(1..652)
                     /locus_tag="WHT44_001143"
                     /pseudo
     CDS             complement(1..652)
                     /locus_tag="WHT44_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222085.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
     gene            complement(857..1165)
                     /locus_tag="WHT44_001144"
     CDS             complement(857..1165)
                     /locus_tag="WHT44_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533332.1"
                     /translation="MDVFPELNIHCFYDDKNQLEAIEIFEPNKFFLEDISFNWLGKIN
                     DEIISYLYKSGINDFEIDDLGIRLLNIPVSTIINSIGKVECCYLDFSNRDTKFYVKQV
                     "
     gene            complement(1616..1939)
                     /locus_tag="WHT44_001145"
     CDS             complement(1616..1939)
                     /locus_tag="WHT44_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533333.1"
                     /translation="MSILEENVIDSASIENGILILTISDHLKWDNEHLFLLQEKINSY
                     IQYIESGQIFEDFGESSYETIEIQLIYKYKPNENYRKFLYRLEGALLKLKLRFSHGTI
                     SYFYS"
     gene            complement(1939..>9094)
                     /locus_tag="WHT44_001146"
     CDS             complement(1939..>9094)
                     /locus_tag="WHT44_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HEZ2533334.1"
                     /translation="ITLISTVEQAGIRNQGQWFASAGNVAVNAEGKLVNTGMIAATGE
                     NHAVSLHARNVHNSGTVASQDDANIHSQTLDNSGTVLSSGRLTVRNLGRLKNQNNGTI
                     QAARLDMSTGGLDNTGNITQTGSQALDLVSAGKFDNSGKIGVSDVPQTGLNPNPSVIP
                     QIPSTATGSGSSTVSVSKPGSNNPVSPTAPAKNYAVGRIQTTGAFDNAGSINAGGQID
                     IAAQNGLGNSGSLNAAKLRVSGDSFNNTVKGKLQAHDLAVNTQTAKNSGHLLTQTGKI
                     DNRELHNAGEIAANNLTLIHSGRLSNDKKGNIRAAHLQLDTAGLHNAGNILADSGTVT
                     TKNNLRNTGKVSVARLNTEGQTLDNTRGRIEAETVNIQSQQLTNQSGHITATEQLTIN
                     SRNVDNQNGKLLSANQAQLAVSDGLYNQHGEIATNRQLSIHDKNQNTLALNNADGTIQ
                     SAGNVSLQAKSLANNGTLTAGNKLDIALTDDFVVERDLTAGKQLNLSIKGRLKNTHTL
                     QAGHTLKLNAGNIDNQVTGKIIGGEQTDITSEQHVDNRGLINSDGLTHIGAGQTLTNT
                     GTGKIYGNHIALDAQILLNREETTEGSTKAGAIAARKRLDIGAKEIHNQEGALLSSEG
                     IFAVGNRLDEQHHAAGMADTFVNGSAGLEVQGDALMSVRNMQNINNHFKTETYLAKAE
                     KQVRDYTVLGQNTYYQAGKDGLFDNSQGQKDQTTATFHLKNGSRIEANQWHVRDYHIE
                     TYKERIIENRPAHITVGGDLTASGQNWLNKDSRIVVGGRIITDDLNQKEITNQSTTGK
                     GRTDAVGTQWDSVTKKGWYSGRKRQRRTERNHTPYHDTQLFTHDFDTPVSVIQQNAAS
                     PSFQPAASAIKLIDGVSTAAVNGQRIHTGNVVSLNNATVTLPNSSLYTTHPDNKGWLV
                     ETDPQFADYRRWLGSDYMLQQLQLDTNHLHKRLGDGYYEQKLVNEQIHQLTGYRRLDG
                     YRSDEEQFKALMDNGLTAAKTFGLTPSIALSAEQVARLTSDIVWMENQTVTLSDGSTQ
                     TVLVPKVYALARKGDLNTSGGLISAEQVLLKLQNGNLTNSGTIAGRQAVLIQARNINS
                     NGNIQADQIGLKAEKSINIDGGQVQAGRLLTAQAQNINLNGTTQTSGNERNGNTAIDR
                     MAGINVVGSHTEQVDNRTSDGILSLHASNDINLNAATVSNQVKDGTTQITAGNNLNLG
                     TIRTEHREAYGTLDDENHRHVRQSTEVGSSIRTQNGALLRAGNDLKIRQGELEAEEGK
                     TVLAAGRDVTISEGRQITELDTSVSGKSKGILSSTKTHDRYRFSHDEAVGSNIGGGKM
                     IVAAGQDINVRGSNLISDKGIVLKAGHDIDISTAHNRYTGNEYHESKKSGVMGTGGLG
                     FTIGNRKTTDDTDRTNIVHTGSIIGSLNGDTVTVAGNRYRQTGSTVSSPEGRNTVTAK
                     SIDVEFANNRYATDYAHTQEQKGLTVALNVPVVQAAQNFIQAAQNVGKSKNKRVNAMA
                     AANAAWQSYQATQQMQQFAPSSSAGQGQNNNQSPSISVSITYGEQKSRNEQKRHYTEA
                     AASQIIGKGQTTLAATGSGEQSNINITGSDVIGHAGTALIADNHIRLQSAKQDGSEQS
                     KNKSSGWNAGVAVKIGNGIRFGITAGGNIGKGKEQGGSTTHRHTHVGSTTGKTTIRSG
                     GDTTLKGVQLIGKGIQADTRNLHIESVQDTETYQSKQQNGNVQVTVGYGFSASGSYRQ
                     SKVKADHASVTGQSGIYAGEDGYQIKVRDNTDLKGGIITSSQSAEDKGKNLFQTATLT
                     ASDIQNHSRYEGRSFGIGGSFDLNGGWDGTVTDKQGRPTDRISPAAGYGSDGDSKNST
                     TRSGVNTHNIHITDEAGQLARTGRTAKETEARIYTGIDTETADQHSGHLKNSFDKDAV
                     AKEINLQREVTKEFGRNAAQAVAAVADKLGNTQSYERYQEARTLLEAELQNTDSEAEK
                     AAFRASLGQVNAYLAENQSRYDTWKEGGIGRSILHGAAGGLTTGSLGGILAGGGTSLA
                     APYLDKAAENLGPAGKAAVNALGGAAIGYATGGSGGAVVGANVDWNNRQLHPKEMALA
                     DKYAEALKREVEKREGRKISSQEAAMRIRRQILRWVDKGSQDGYTDQSVISLIGMKGE
                     DKALGYTWDYRDYGARNPQTYNDPKLFEEYRRQDKPEYRNLTWLHSGTKDTKIRQGER
                     KNEEFALNVAEGLTSLVNPNPRIKVPILAGIRNLKNIKPTVTGSDPLLAGAGNIRIPA
                     NGNVAKGDRIPDTALASKGIKHKNRKDQLEKNKKSGEDFEMEIYQKKVKQGFKPQRQI
                     TVKTKSGVKTRLDIISKEGGLDVCTECKASITAPLTKNQKKAFPEIERTGATVVGKGK
                     PGYPKGTKIEPTKVIIERKR"
BASE COUNT         1847 a         2072 c         2196 g         2979 t
ORIGIN      
        1 ccgtagccgg ctgccaggct tatcctgtcg gtaggtctgc cttgtttgtc ggtaaccgtg
       61 ccgtcccagc cgccgttcag gtcgaaactg ccgcctatgc cgaagctttt gccttcgtag
      121 cggctgtggt tttgaatgtc gctatgggtg agggtggccg tctgaaaaag gtttttgccc
      181 ttatcttctg cgctttggct agacgtgatg ataccgccct tgaggtctgt gttgtctctg
      241 actttgattt gatagccgtc ttctccggca taaataccgc tttgctcggt taccgaagca
      301 tggtcggctc ggattttgct ttggctgtaa tcgccactgg cactgaagcc ataacctacg
      361 gtcacttgtg cactggcgtt ttgttgtttg ctttgatagg tttcagtatc ttgaacactt
      421 tctatatgca ggttgcgcgt atctgcctgt atgcctttgc cgatgagctg cgcacctttg
      481 agggtggtat ccccgccgct tcgaatggta gttttaccgg ttgtgctgcc gacatgggtg
      541 tggcggtggg ttgcttttgt ctgattgctg tggtggcttg ttgaaagaaa ggctgtctga
      601 aacgtatttg ttgtttcaga cagcctcctg gctcaaacct tgaaaactac atatgtgcgt
      661 tccgcacatc ctacgtattg agtttaggtt tcacatgagc tacggcttgc tatgccgtct
      721 tttttccagg tgtggccgcg gctttggttt tcggcaaggt aggcgtttac ttggtcagat
      781 ggcggatttt ttgttcgtcg tagatgatgg agacgctgat accggagtaa gcgtagattg
      841 ggtcttgacc tcaaacctac acttgtttta cataaaattt cgtgtctcta tttgaaaaat
      901 ctaaataaca acattctact ttacctattg aattgattat agttgaaaca ggaatattaa
      961 gaagcctaat acccaaatca tcaatttcaa aatcattaat tccactctta taaagatagc
     1021 ttattatttc atcattaatt tttccaagcc aattaaaaga aatatcttct aaaaaaaact
     1081 tatttggttc aaatatctct atcgcttcaa gctgattttt atcatcataa aaacaatgga
     1141 tattcaattc gggaaaaaca tccataggaa cccgagagta tgatgactta taaatttctt
     1201 gtacatcaga actaaatatt gcacgaactt gttttcgatt cagatttgat gagataatat
     1261 ttctttctat tttttttgaa ctaaaataaa aaattgccat aatttttttg cctaaacaat
     1321 attaccattt tcgtaagatg catagaacaa accatgtctt atccattttg ttccatcggc
     1381 agacagataa cgactatatc taaattttat ttttcactct cataaaaaat tttctgcaat
     1441 attcaatata tttactttct taaccatagc gtaaattcct caggcttata tatttcagta
     1501 taagtatgac ttaaaggata tgacgccgcg tgttacgagt tgcttctttt gatttcaggg
     1561 tttatataag ttatggcttg cctgggctcg aagtgataaa gagagtattt acttttcaac
     1621 tataaaaata tgagatagtt ccatgggaaa accgtaattt aagttttaat aaagcacctt
     1681 ctaggcgata taaaaatttt ctataatttt catttggttt atatttatat ataagctgta
     1741 tttcaatagt ctcatagcta ctttctccaa aatcttcaaa aatttgacct gattcaatat
     1801 attgaatata tgaatttatt ttttcttgca acaaaaacaa atgctcgtta tcccatttta
     1861 agtgatcaga aatagttaat atgagtattc cgttttcaat agaggctgaa tcaattacat
     1921 tctcttccaa aatagacatt atcttttcct ttcaattata actttagtag gttcaatttt
     1981 ggtccccttt ggatagcccg gttttccctt accgaccact gttgctcccg ttctttcaat
     2041 ttcaggaaaa gcttttttct gatttttagt aagtggcgca gttattgaag ccttacactc
     2101 tgtacaaaca tcaagaccac cttctttcga aataatatca agcctagttt ttacaccact
     2161 ttttgtttta actgtaatct gtctttgcgg tttaaagcct tgtttaactt tcttctgata
     2221 aatttccatc tcaaaatcct caccagattt tttatttttt tccagttgat ctttacgatt
     2281 tttatgtttg attcccttgc tagccaatgc cgtatccgga atcctgtccc ccttcgcaac
     2341 attgccgttt gcagggatac ggatattccc cgcacccgcc aataagggat cgctgccggt
     2401 aactgtcggc ttgatgtttt tcaggttgcg gatgcctgca agaatcggga cttttatcct
     2461 cggattgggg ttgacaaggc tcgtcagtcc ttcggcaacg ttcagtgcaa actcttcgtt
     2521 tttccgctct ccctgcctga ttttggtgtc tttcgtcccg ctgtgcagcc aggtcaggtt
     2581 gcggtattcg ggtttgtcct gtcggcggta ttcctcaaac agcttcggat cgttgtaggt
     2641 ttgcggattt cttgcgccgt agtcgcggta gtcccaagta taacccaagg ctttgtcttc
     2701 gcctttcatt ccgataaggg atatgacgct ttggtcggta tagccgtctt gggaaccttt
     2761 gtccacccaa cgcagtatct gcctgcggat tctcattgcc gcttcttggc tgctgatttt
     2821 tctgccttcg cgtttttcaa cttcgcgctt gagggcttcg gcatatttgt cggccaacgc
     2881 catttctttc ggatgcagct gcctattgtt ccaatctaca ttcgcaccca ccacagcacc
     2941 accactacca ccagttgcat agccgatggc cgcaccgccc agtgcgttga ccgccgcttt
     3001 gcccgccgga ccgaggtttt ccgccgcttt gtccaaatac ggtgcggcaa gggaagtgcc
     3061 gccgccggcc agtatgccgc cgaggctgcc ggtcgtcagt ccgcctgccg ccccgtgcag
     3121 tatgctcctg cctatgccgc cttctttcca ggtgtcgtag cggctttggt tttcggcaag
     3181 ataggcgttt acttggccga gggatgcgcg gaaggcggct ttttcggctt cgctgtccgt
     3241 gttttgcagt tcggcctcca gcagggttcg ggcttcctga taccgttcgt aactttgggt
     3301 attgccgagt ttgtcggcaa cggccgctac ggcttgggcg gcgtttctgc cgaactcctt
     3361 cgttacttcc ctttgcaggt tgatctcttt ggcgaccgcg tctttgtcga agctgttttt
     3421 cagatggcct gagtgttgat ccgcagtttc ggtgtcgatg ccggtgtaga tacgcgcttc
     3481 ggtttctttt gcagtcctgc ctgttcgggc aagttgtccc gcttcgtcgg tgatgtgtat
     3541 gttgtgggtg ttgacgccgc tgcgggtggt gctgtttttg ctgtctccgt cgctgccgta
     3601 gccggctgcc gggcttatcc tgtcggtagg cctgccttgt ttgtcggtaa ccgtgccgtc
     3661 ccagccgccg ttcaggtcga aactgccgcc tatgccgaag cttctgcctt cgtagcggct
     3721 gtggttttga atgtcgctgg cagtaagggt ggccgtctga aaaaggtttt tgcccttatc
     3781 ttctgcgctt tggctagacg tgatgatacc gcccttgagg tctgtgttgt ctctgacttt
     3841 gatttgatag ccgtcttctc cggcataaat accgctttgc ccggttacgg aggcatggtc
     3901 tgctttgact ttgctttggc ggtaactgcc gcttgcactg aatccgtaac cgacagtaac
     3961 ttggacattg ccgttttgct gtttgctctg ataggtttca gtatcttgaa cactttctat
     4021 atgcaggttg cgcgtatctg cctgtatgcc tttgccgatg agctgcacac ctttgagggt
     4081 ggtatccccg ccgcttcgga tggtagtttt gccggttgtg ctgccgacat gggtgtggcg
     4141 gtgggtagta cttccccctt gctctttacc tttaccgata tttcctccgg cggtaattcc
     4201 aaacctgatg ccgttgccta ttttgacggc tacgcctgca ttccaaccac tgcttttgtt
     4261 tttgctttgc tcgctgccgt cctgtttggc agattggagt ctgatatggt tgtcggcaat
     4321 gagggcagta cctgcatggc cgatgacatc ggaacctgta atattgatat tggactgctc
     4381 cccacttcct gttgccgcaa gtgtggtttg ccctttgccg ataatttgac ttgctgccgc
     4441 ttcggtgtaa tgtctttttt gctcgttacg acttttctgt tcgccgtagg taatggacac
     4501 actgatactg gggctttgat tgttgttttg accttgtccc gcactgctgc ttggagcaaa
     4561 ttgttgcatt tgttgggttg cttgataact ctgccatgca gcattggctg cagccatggc
     4621 attaacgcgt ttatttttac ttttgcccac attttgggct gcttgtatga agttttgtgc
     4681 agcttggaca accgggacat tgagggcgac ggtaaggcct ttttgttcct gggtatgggc
     4741 gtagtcagtg gcataccggt tgtttgcgaa ctctacatct atgcttttgg ctgtgacggt
     4801 attgcgcccc tcggggctgg agacggtact gccggtttgt cggtagcggt ttcctgcaac
     4861 tgtaacggtg tctccattca ggctgcctat aatgctgcct gtatggacaa tattggtacg
     4921 atcagtgtca tcggtagttt tccggttacc gatagtaaag cccaatccgc cagtacccat
     4981 gacgcctgat tttttgctct cgtggtattc attgccggta tagcgattat gggcagtaga
     5041 aatatcgatg tcgtgtcctg cttttaaaac aatgccctta tcagaaataa ggttgctgcc
     5101 gcgtacattg atatcctgcc cggctgcaac aatcattttg ccgccgccga tgttgctgcc
     5161 gactgcttca tcatgactga agcggtagcg gtcgtgtgtt ttggtactgg aaaggatgcc
     5221 tttgcttttt ccgcttaccg aggtatccag ttcggttatt tggcgtcctt cgctgatagt
     5281 gacatcacgt cctgcggcaa ggacggtttt gccttcttcg gcctccagtt cgccttggcg
     5341 gatttttaag tcgttaccgg ctctaagcag tgcgccgttt tgcgtgcgga tactgctgcc
     5401 gacttcggta ctttggcgga catggcgatg gttctcgtca tctaatgtac cataggcttc
     5461 gcgatgttcg gtacggatgg tgccgaggtt gagattattg ccggcggtaa tttgggtagt
     5521 gccgtcttta acttggttag agacggtggc cgcattgagg ttgatatcgt tgctggcatg
     5581 cagggatagg atgccgtctg aagttctgtt atctacttgt tcagtatggc ttccgaccac
     5641 gttaatgccg gccatacgat cgatggcggt attgccgtta cgttcattac cggaagtttg
     5701 ggttgtaccg ttaaggttga tattttgcgc ttgggcagtc agcagtctgc ctgcttgtac
     5761 ctgcccgccg tcgatattga tacttttttc agcttttaag ccgatttggt cggcttgaat
     5821 gttaccgttg ctgttaatat tccgtgcctg gatgagtacg gcctgtcgcc ccgcaatggt
     5881 accgctgtta gtcaggttgc cgttttgcag tttaagtaag acttgttcgg cactaatcag
     5941 gccaccggag gtattgagat cacctttgcg cgccagggca tagactttag gaaccagtac
     6001 ggtttgagtc gaaccgtcag acagggtgac ggtttgattt tccatccaaa cgatatctga
     6061 agttaagcgg gcaacttgct ctgcactcaa ggcgatactt ggggtgagac cgaatgtttt
     6121 ggcagcagta aggccgttgt ccatcagagc tttgaattgt tcttcatcac tcctgtagcc
     6181 gtcgagtcgg cggtagcctg ttaactgatg gatttgttca ttaacaagtt tttgttcgta
     6241 gtagccgtcg ccaagccgtt tgtgtagatg attggtgtcc aattgcagtt gttgcaacat
     6301 gtagtcgctg cccaaccagc ggcggtagtc tgcaaattga ggatcggttt caaccaacca
     6361 gcctttattg tcaggatggg tggtatagag gctgctgtta ggcagagtaa cagtagcgtt
     6421 atttaacgag accacattac cggtatggat gcgctgacca ttgacggctg ccgtggatac
     6481 tccgtcaatc agtttgattg cagatgcggc gggttgaaag gaaggggagg cggcattctg
     6541 ttggatgacg gatacaggcg tgtcgaagtc gtgggtaaat agttgggtat catggtaagg
     6601 agtatggttt ctttcagtac ggcgttgtct ttttctaccg ctgtaccatc ctttttttgt
     6661 aactgaatcc cactgtgtgc cgacagcatc tgtgcgacct ttgcctgttg tactttgatt
     6721 ggtaatttct ttctggttta aatcatcagt gataatacgc ccgcctacta caatccggct
     6781 gtctttgttc agccaatttt gacctgaggc agtcaaatca ccgcccacag taatgtgtgc
     6841 cggccggttt tcgatgatgc gttctttata agtctcgatg tggtagtctc ggacatgcca
     6901 ttggttggcc tcaatacgag aaccattttt taaatggaac gtagcagtag tttggtcttt
     6961 ttgtccttgc gagttgtcga ataaaccgtc ttttcccgcc tgatagtagg tattttgccc
     7021 cagtacggtg tagtcgcgga cttgcttttc cgctttggct aagtatgtct ctgttttaaa
     7081 gtgattattg atattctgca tattccgaac ggacatcaat gcatcacctt gtacttccaa
     7141 accggcactg ccattaacaa aggtatcggc catgcctgcc gcatgatgtt gttcatccag
     7201 tcgattacct acggcaaaaa taccttcgct ggatagtagg gcaccttctt ggttatgaat
     7261 ctctttcgct ccaatatcca aacgtttcct tgcagctatt gcccccgctt tggtactgcc
     7321 ttccgtcgtt tcttcccggt taagcagtat ttgcgcgtcc agggcaatat ggttgccata
     7381 gattttgcct gtcccggtgt tggtcagggt ttgacctgca ccgatgtggg tcaaaccgtc
     7441 gctgttgatc aagcccctgt tgtcaacatg ctgttcggat gtgatgtccg tttgttctcc
     7501 accaataatt ttgcctgtaa cttggttatc tatattgccg gcattgagtt tgagcgtatg
     7561 gcctgcttgt agggtatggg tatttttcag acggcctttt atgcttagat ttaattgttt
     7621 gcctgcagtg aggtcgcgct ctacgacgaa atcgtccgtc aaagcaatat ccagtttgtt
     7681 accggctgtt aatgtgccat tgttggcgag tgatttggct tgtagcgata cattaccggc
     7741 agattgaatc gtgccatccg cattgtttaa cgccaaagtg ttttgatttt tatcgtgaat
     7801 agacaactgc cggttggtgg caatttcacc atgttggttg tataggccgt ctgaaacagc
     7861 taattgtgct tggtttgcag ataggagttt gccgttttgg ttgtctacat ttcgactatt
     7921 gatagtcagt tgttcggtag cagtaatatg gccgctttgg ttagtcagtt gctgactttg
     7981 gatgttaacc gtttcagcct ctatacgtcc gcgcgtatta tctagagtct gaccttcggt
     8041 attcagtcgt gcaacagaaa cttttcctgt attgcgaaga ttattcttgg tggtaacggt
     8101 tccactatcg gcaagaatgt tacctgcatt atgtaaaccg gcggtatcaa gctgtaaatg
     8161 tgcagctcga atattgcctt ttttatcatt gctcaagcgg cccgaatgaa tgagtgtcag
     8221 attgttggcg gcaatttctc cggcattatg cagttcacgg ttatcaatct tgccggtttg
     8281 agttaataag tgaccgctgt ttttagcagt ttgagtgtta acagccagat cgtgtgcctg
     8341 gagtttgcct tttaccgtat tgttaaatga atcgcctgat actcgtagtt tagccgcatt
     8401 cagactaccc gaatttccca aaccgttttg ggcggcaatg tcaatttgcc cacccgcatt
     8461 aattgatcct gcattgtcaa atgctcctgt tgtttgaatg cgtcctacgg cgtagttttt
     8521 tgcaggtgct gtaggtgaaa cgggattgtt tgaaccaggc ttagataccg agacagtgct
     8581 gctgcctgaa cctgttgcag tactcggaat ctgtggtatg actgatggat tgggattcaa
     8641 accggtctgt ggaacgtcac ttacaccaat cttgccactg ttatcgaatt tgccggcaga
     8701 taccaaatcc aatgcttgtg aacctgtttg agtaatatta cctgtgttat ccaaaccacc
     8761 tgttgacata tctaagcggg cagcctggat cgtaccgttg ttttggtttt tcagacggcc
     8821 taaattacga acagtcaatc gacctgagga taagaccgta cctgaattgt ccagcgtctg
     8881 gctgtgaata ttggcatcat cctgtgaggc aaccgtaccg ctattatgaa cattgcgggc
     8941 atgaagtgaa accgcatgat tttctcccgt cgctgcaatc atgcccgtgt tgaccagttt
     9001 accctcagca ttcactgcca cattgccggc tgaggcaaac cattgccctt gattacgaat
     9061 gcctgcttgc tcgaccgtac tgatcaaggt gatt
//
LOCUS       DAWGRZ010000069         9070 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000069 DAWGRZ010000000
VERSION     DAWGRZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.069"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            141..323
                     /locus_tag="WHT44_001147"
     CDS             141..323
                     /locus_tag="WHT44_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533335.1"
                     /translation="MPTPPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQN
                     QAWRDWAGNHSRKVGN"
     gene            complement(368..538)
                     /locus_tag="WHT44_001148"
     CDS             complement(368..538)
                     /locus_tag="WHT44_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ2533336.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            551..769
                     /locus_tag="WHT44_001149"
     CDS             551..769
                     /locus_tag="WHT44_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533337.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            766..1104
                     /locus_tag="WHT44_001150"
     CDS             766..1104
                     /locus_tag="WHT44_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533338.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            1116..1382
                     /locus_tag="WHT44_001151"
     CDS             1116..1382
                     /locus_tag="WHT44_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533339.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKADAKADSTNIAAALERIENKLEKVNEKLDRKADKS"
     gene            1379..1576
                     /locus_tag="WHT44_001152"
     CDS             1379..1576
                     /locus_tag="WHT44_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533340.1"
                     /translation="MSDPILDALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            1579..2085
                     /locus_tag="WHT44_001153"
     CDS             1579..2085
                     /locus_tag="WHT44_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ2533341.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     VEVEKKFG"
     gene            complement(2082..2672)
                     /locus_tag="WHT44_001154"
     CDS             complement(2082..2672)
                     /locus_tag="WHT44_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533342.1"
                     /translation="MNWLNRGIQEKILRVLSDNYPNCLKATKVYNSLFPSSSLPITSN
                     IPVDKQGLGLALTLGCEIQSQHLATLQSDEFQYFLKNIYYLEESGLIEITSIDSLHKN
                     FDCKINHKGIDFLTDDGGLSAILGVVTVKLHSDTIQALIAAKIDQAEISDSEKSWLKK
                     ELGKIKDTALSTLTENAINAIPAATLITLLKKFVGL"
     gene            2685..4307
                     /gene="terL"
                     /locus_tag="WHT44_001155"
     CDS             2685..4307
                     /gene="terL"
                     /locus_tag="WHT44_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ2533343.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTK
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTVLDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYQANKDEMERGAVTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRVTG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            4307..5875
                     /locus_tag="WHT44_001156"
     CDS             4307..5875
                     /locus_tag="WHT44_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ2533344.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     NGTTATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPHADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPVNRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            5862..7157
                     /locus_tag="WHT44_001157"
     CDS             5862..7157
                     /locus_tag="WHT44_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEZ2533345.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLSNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKETQELAGM
                     TRATVWLSDDTLVKQVDSREGQNFDDSYYAFLPDMLQNPEHVIRDNRELIFTARYKGS
                     ALWAVLKYIKEVDEIYLQSYRISNDKEIAKFMAKKKVLK"
     gene            7267..7682
                     /locus_tag="WHT44_001158"
                     /pseudo
     CDS             7267..7682
                     /locus_tag="WHT44_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
     gene            7913..8974
                     /locus_tag="WHT44_001159"
     CDS             7913..8974
                     /locus_tag="WHT44_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533346.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFDLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAKGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
BASE COUNT         2401 a         2133 c         2480 g         2056 t
ORIGIN      
        1 taaaaaggaa atcgaaaatg tccttactca agaccgtaaa aatgcaagcg gcggttgcat
       61 tgacggcttt ggctctcacg gcctgcagct ctacaaccgc gccctcggct acggaaatta
      121 aggttgtcga aaaggcagtc atgccgaccc cgcctgctgc attgatggtc gcgccggtac
      181 gcccgaatcc gccgaaagac ggcaagacgg ccacgctgtt ggaacacgcc gctgagtttg
      241 gtggctatgt ggcggagttg gaaaatcaaa atcaggcttg gcgcgactgg gcgggcaatc
      301 actcccgcaa agtcggaaac tgacaaaaaa gcccgcgtag ggcgcgggct gagggtgaaa
      361 gcggatttta tacctctttt acaggggtag cggcggtagt gcttttcagc aaatcgactg
      421 cgtgctgaca gttttgcttg ctggtgtagc cttcgccctg agcgatgatt tcatggttgg
      481 ctgctttcaa acgccaacgg tattcgcctt ttgcgtcttt atagatttca aaatacataa
      541 ggtttctcct atgaatgagt acacgttttc ttaccgcttt aacggcaagt cctggtcatt
      601 gagcatttgg gcggacaacc ctgaagaagc cagggcgaaa tttcgggctg cacgagaaaa
      661 tgcgcactat gacggcgaag ttgtagcaaa ggtttataca tttgtaaata tttcgtgggt
      721 taagaaattg tacaagcgga caaaatattt aatgggtatc aaagaatgac ctaccgtgaa
      781 ttagttgaac gtcagttggc tgtgcgccat gccgatttgg aattgggctt aagccgcgca
      841 cgcgagcaag agccgtttgt cattcatgtt tccgatctgt tggataaggc aggcattgag
      901 tacgcggtac gcatggataa ggattttcag acgacgtttc accttgaata tccaattacg
      961 aactatgaca cctttaaacg tgcggtttgg caaactttgg gggcgtatta ctgtgtttgt
     1021 aatgatggtg atggactgga gattgccagc aatcgccctg acggttacgc cgtccgtatc
     1081 gtattcggcg atgtgccggt ttaaaggggt tttaaatgga ctttgaattt ggtttcagaa
     1141 ccctgtggcc gattgcgacg gcggcatttt ggttttgggt caacggcatt tcaggccgcc
     1201 tgaaagaggc ggacaagcgt atcgacgacc ttaaagagga gttgcacgcg gtcaagctct
     1261 cttatcacac caaggcggac gccaaggcag acagcactaa tattgcggcg gccttggagc
     1321 gaattgaaaa caagttagaa aaagtaaacg aaaaactgga caggaaagca gacaaatcat
     1381 gagcgacccg attttggatg ccttggcgcg tattgaaaac aagactgatc aaacgctgaa
     1441 aaatcagaag gaaatgcagg cggaaattgc gcaaattcgc caagacacga aacgcacggc
     1501 cattacattc ggcgcactgg gcggcggcgt gattacggtc ggctgggaat tgcttaaagc
     1561 gaaaatggga ctgtaattat ggctcacccg caagaaatcc gtgaaaagtt acgccggctc
     1621 tatgtgagcg gcgagcaaac tttggaaacg gcggccttga tgtgcgaaat cccgcagacc
     1681 actgcgcgtg cgtggaaacg tgcggataag gaaaaaggcg acgactggga taagatgcgc
     1741 gccgcttaca ctttggccgg tggcggtatt gaggatttga gccgtgcgat gttggccggt
     1801 tttatggtgc agtacaacag cacgatgacg atgctgcagg attcgagtac cgaagatttg
     1861 ccaccatccg accgcgccaa gctgttggcc agcctggccg atgcgtttac gaaaaccgta
     1921 tcggccaatg cgcgtgtgat gccggaaacg tcaaaactgg cgacggcttt ggaattgatt
     1981 gagttcttga tggcgtttgt acaagaaaaa catcccaaac atttgcctgc ctttgtggag
     2041 gtattggagc cgtttggggt ggaagtggag aagaagtttg gttagaggcc aacaaatttt
     2101 tttaaaagag taatgagggt ggcggcaggg atagcattaa ttgcattttc cgtaagcgta
     2161 cttaatgcag tgtccttgat tttccctaat tcttttttca accagctttt ttctgaatca
     2221 gaaatctctg cttggtctat ttttgccgca attaaagcct gaatagtgtc gctgtgtagt
     2281 ttgactgtga caacaccaag aatggcggat aggccgccat catcggtaag gaagtctatg
     2341 cccttatgat taattttgca gtcaaaattt ttatgaagtg aatctatcga agtaatttca
     2401 attaaaccag attcttctaa gtaataaata ttttttaaaa aatattggaa ttcatctgat
     2461 tgtaaagttg ctaggtgttg actttgaatt tcacaaccaa gagtcaaagc caagcccaat
     2521 ccttgtttat caacagggat gttagaagta ataggtaaag aactactagg gaaaagagag
     2581 ttatacacct tggttgcttt taggcagttc gggtaattat cacttaaaac tcgtaagatt
     2641 ttttcctgaa tacctctatt taaccagttc ataaattatt cctcatgaaa acaaaagaat
     2701 tcctcaaatc ccttgccgaa ctggccgcca gtttgcgcca agtcatcgaa gcggaagtgg
     2761 acggcttcga tgcgtcgccc aaggctattg ctgcacgccg tgccaaggtg tttgacccgg
     2821 taggcggtta cgagtatttc gtgaatacct acttccccca ttatatccgc tcgcctgaga
     2881 aatccgaact gcatgcgttt ttattcagcc gtctgccgga gattatccgc tcccccaaag
     2941 gggaaaatga ggcggtgggt gcgccgcgtg gagagggtaa gtcgacgaag gttactcagt
     3001 tgtttacgct gtggtgtatt gtgaccggcc aaaaacatta tgctgttatt gtgatggaca
     3061 gtatcgacca ggcatatccg atgctggaag ccatcaaggc ggaacttgaa tttaacccgc
     3121 gcttgaaaac cgactttccg gaagtatgcg ggcagggccg tgtatggcag gccggtacga
     3181 ttgtgacggc caatgacgtt aaagtccaag tggccggtag cggtaaaaag ctgcgcggtt
     3241 tgcgtcacgg cccttaccgt cctgacttaa ctgttttgga cgatattgag aatgacgagc
     3301 aagtccgcaa ccccgaacag cgcgacaagc tcaatgcgtg gctgactaag accgtattgc
     3361 ctctgggcgg tgtcggtcag aaatacgatg tgatttatat cggcacgatt ttgcattacg
     3421 acagcgtact taaccgcact ttgaataacc cgttttggca cggtattaag tttaaggcga
     3481 tgaaacgctg gcctgaccgc atggatttgt gggacaggtg ggaggaactt ttccgaaacg
     3541 acggcgagac ggtggccgag gcgttttatc aggcaaacaa agacgagatg gagcgcggcg
     3601 cggtcacttc ttgggcggcg cgtggcgtac tcgcgctgat gaaaatccgt gcgcgtgacg
     3661 gccatgcgac gtttgattca gaatatcaaa acgatccggt cagtggcgaa gatgcgccgt
     3721 ttgccaagtc gatgaagttt tggaacgacc tgccgtccga tttggtgtat ttcggtgcgc
     3781 tcgacccgtc actcggaaag gccggggcga gccgtgaccc gtccgcgatt atcattggcg
     3841 gttatcaacg tgtaaccggc aaactgtatg tcgtggaggc tcagattaaa aaacgtctcc
     3901 ctgatttgat tattgaggac gttattcgat tgcaccgtca atatcgttgc aaactgtggt
     3961 ttgttgagac tgttcaattt caggaatttc tgaaagacga gctggtcaag cgcagcgcgg
     4021 cgcgtggaat acctgtccca gcgcgggcgg tcaaaccggt atcggacaag ctgttgcgga
     4081 tcgagacttt acagcctcac atggcgaacg gtttgattct gttgaatgag agccaacaaa
     4141 cgctgataca gcagttccgc cattttccaa aggctgatca tgatgatggt cctgatgccg
     4201 tgcatatgct ctggtcgggg gcggtggcca attgtgtgcc gatagaatgg caaagcccta
     4261 ccgataacga ttttgatgac gagataaaaa gtaaatggag ccgataatgg caaaaaagaa
     4321 caataaaact aaaatccaaa agcccgaagc tgcattgcag acggacgtgg ctcaaattac
     4381 ggcgaccggt cgggttatcg ccgagcatcc gtccaatttt attacgccgc aaaagatgcg
     4441 ggccctcttc gaggacgcag aaagcggcga catccgcgcc caacacgagc ttttcgcgga
     4501 cattgaggag cgcgacagcg acatcgcggc aaatatgggg acgcgcaaac gcgcgctgct
     4561 gacgctcaac tggcgcgtcg ccccgccgcg aaatgcgacg cccgaagaag aaaagctgtc
     4621 cgaccaagcc tacgaaatga tggacagcct gcctaccctc gaagacctga ttatggattt
     4681 gatggacgcg gtagggcacg gattttctgc gttggaggtc gagtgggtat tttcagacgg
     4741 cctttaccta ccccgaaact ttatccaccg cccgcaaagc tggttcaaat gggacaaaga
     4801 caacgggctg ctgctgcgta cccgcgaaaa tccggaaggc gaagcgttgt ggccgctggg
     4861 ctgggtcgtt catacccaaa aatcgcgcag cgtccagcag gcgcgcaacg ggcttttccg
     4921 cacgctttcc tggctgtata tgttcaaaca ctacgccgtc cacgattttg ccgagttttt
     4981 ggagctgtac ggcatgccca tccgtatcgg caaatacggc gcgggcgcaa ccaaagagga
     5041 aaaaaacacc ctgcttcgag cggtggcgga aatcggtcac aacgcggcag gcatcatgcc
     5101 agaaggtatg gaaatagagc tccacaacgc ggcaaacggt acgacggcaa ccagcaatcc
     5161 gtttttgcag atggccgact ggtgcgaaaa atcggcggcg cggctgattt tggggcaaac
     5221 gctgaccagc ggtgcggacg gaaaatccag caccaacgcg ctgggcaata tccacaacga
     5281 ggtacgccgc gatttgctgg tgtcggacgc aaaacaggtg gcgcaaacca tcacaagcca
     5341 aatcatcgga ccgttcctgc aaatcaacta tccccatgcc gacccaaacc gcgtgccgaa
     5401 atttgaattt gacacgcgcg agccgaaaga catcgcggtc tttgccgacg ctatcccgaa
     5461 actggtggat gtcggcgtac aaatccccga aagctgggtg cgcgacaaac tggtcattcc
     5521 agatgtgcag gagggtgagg ctgtgttggt gcggcaggta ccggacaatc cggtaaacag
     5581 aactgcattg gcggctttat ccgcccacac cgtaccatct aaggctacgg gcaggcatca
     5641 ggaaatattg gacggcgcgt tggatgacgc gctggttgag cccgatttca attctcagct
     5701 caacccgatg gtgcgtcagg cggttgccgc acttaatgct tgcaacagct acgaggaggc
     5761 agatgccgca ctgaatgcgc tttatccgaa tttggacaac gcgaaactgc gtacctatat
     5821 gcagcaggcc ttgtttatca gcgatatttt gggacaagac catgcccgcg cctgatttgg
     5881 gatttgcctt aagtctgccg ccaaaaaagg caatcgagtg gctggaaagt aaaaaggtta
     5941 cggcggagag ctaccgcaat ctgacagcct ccgaaattgc caaagtctat acgattgccc
     6001 gcatgaccga cttggatatg ctcaacgaca tcaaaacttc gatggttgaa tcggcaaaaa
     6061 gtggacagtc gtttgacgat tggcgaaaag gtatcttgaa tctgctcagc aacaagggct
     6121 ggctgcatcc gaacgggcat aacggtaagg atatcatcga cccagccacc ggcgaggtat
     6181 tcggttcgcc gcggaggttg gagacgattt accgtaccaa tatgcaaact gcctacaacg
     6241 ccggtcaata tcaaggatat atggcaaata ttgatgcacg accttattgg atgtatgacg
     6301 cggtaggcga cagccgcacc cgtccggcgc attcggcaat agacgggctg gtgtaccgct
     6361 acgacgaccc gttttgggca acgttttacc cgcccaacgg ctacaactgc cgctgctcgg
     6421 tcatcgcgct gtcggagcgg gatgtggaac gccaggggcg gattgttggg caaagcacgg
     6481 cggacaatct ggtcgagacc cataaaatct acaacaaaaa aggcgatact tatctgaccc
     6541 ttgcctataa agcaccggat ggcagtctgt acacgaccga tcgaggattt gattacaacg
     6601 ccggacgaat gaactaccgc cccgatttag acaagtacga ccgtgcgttg gcgcatcaat
     6661 ttgccaaagc ggaaatgggt ggtgcggatt ttaaaaccag ctttaaacag cttgaaaaag
     6721 agttttatga agtcaagcaa cgtttggata ttgatggcaa gcccgataaa gagcagaaaa
     6781 tcaaaatccg aaatgcgcta tcaagacagc ttaaatttgc tgcgggtgta ttgagcaagg
     6841 aaacgcaaga attggcaggt atgacacgag cgacggtgtg gctgtctgat gatacgttgg
     6901 ttaaacaggt agacagccgt gaggggcaga atttcgatga ctcctactat gcttttttgc
     6961 cggatatgct gcaaaaccct gaacatgtca tccgcgacaa tcgtgaattg attttcacag
     7021 ctcgctataa aggctcggca ttgtgggcag ttttaaaata tattaaggag gtggatgaga
     7081 tttatctaca gtcgtaccga atcagtaacg acaaagagat tgccaaattt atggcgaaga
     7141 agaaagtatt gaaatagacg ttgggcaagg ctcgaaatca cttgcacacg ctctcggacg
     7201 ccctaacggg caggctgcgg tatcgaggtt atcaccgctt ttccaacgtc tgtacggaat
     7261 aataccatga ttgatgtcaa aatagacaat atctttgtcg tcctaaacca aatcgagcgg
     7321 cttggcaacg ggatcgaaaa ccgctacctg ctgatgcgcc gactgtccga aaccatgcac
     7381 acggcggtca agctcaattt ccgctacgca ggccgtccga aatggttggg ctaaaatacc
     7441 gcgacggcaa gccgctttcg gattcgggtc gtctgaaaga cagtttttcc acactgtcag
     7501 acaacgatac agcccttgtc ggtacgaata tcgtctatgc cgccatccac aacttcggcg
     7561 gtatggcggg gcgcaaccgc aaagttcgga ttccgcaacg ggaatttttg acgctgacgg
     7621 acgacgacaa acaggctttg atggacgatg tgcaggatta tttttcgggt ctgataccgt
     7681 gaatttataa aaccctcaaa aacgcgcttt ttagcgcgtt tttttatgcg ggtaatacaa
     7741 acccctgccc aagatataaa aatcaatcct agacgcttct aaaaagcccc tgaaaacgat
     7801 taattgtgta tcgcgcggac aggttttaaa aaaatggcgg gagggtttga agcacgccta
     7861 ctctttgttg ttttttcaaa taggcaaaat gaccgtattg agagaggtac acatgtccaa
     7921 aaatgcacaa aaaaccctac ttgccgtgtg cagtttcgag gtgcagccaa aagacgggcg
     7981 aatccaactg ctgccatatg gcgaatttcg cgcagtagac ggtcgtccga ctgatgtccc
     8041 tgcgtggtat ctgaccgaag aaaacggtca tgatgtcgcg ttgttggcca acagctcgcg
     8101 caatcagttg gttgtcgatt atgaacacca gacgctctac aaagagaaaa acggacaacc
     8161 tgcacctgcc gccggttgga tgcgttggct ggagttcacg cctaaaggca tgtttgccga
     8221 agtggagtgg acggacaagg cggctgcggc aattgccgca aaagagtatc gctacatctc
     8281 tgctgtgttt tcctatgaca caaagggata tgtaagcaaa atttttcacg ccgcgctgac
     8341 aaatttcccc gcgttggacg gtatggacga ggtgctggcg gcagcgtcgg cgcaaatttt
     8401 aaaaccggaa acggagcaaa accctatgaa agagttgtta cagcaactgt tcgacctgcc
     8461 tgatgcgggc gaagaagaac tgaaggcggc attgtccgcg ctcgtggaag ccaagccgaa
     8521 agacgtggca ttgtctgccg acgtgttcgc gcagctggcg gaaaaagaca gccgcatcgc
     8581 ggcattgacg gcgcaaaccg ccaagcctga tttgactaaa tacgcgccta tctcagtggt
     8641 tcaagagctg caaagcaaag tcgccgcgct gactgccaag caggaagcag acaaaggcaa
     8701 cgaattgatt accgccgcgc tgacttcagg caaattgctg cctgctcaga aggagtgggc
     8761 aaaaggcgta ttgaaacagc cgggcggctt ggcatttttg accggcttta ttgaaaacgc
     8821 ccagccggtc gctgcactgg caggctcgca aacgggcggc aaagcacccg acgaacgcgt
     8881 cgccgcactg actgcggaag aggcagccgc agcaaaaatg ctgggcatgt ccggcgaaga
     8941 atttgtaaaa atcaaagaaa gcgaaggtaa gtaatggaca aatcagcgat tttgaccgca
     9001 atcacggcag cattccgcaa agaatttcaa acggccttgg attcggattt caagtgcaac
     9061 actaaggtac
//
LOCUS       DAWGRZ010000070         8951 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000070 DAWGRZ010000000
VERSION     DAWGRZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8951
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.070"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(175..867)
                     /locus_tag="WHT44_001160"
     CDS             complement(175..867)
                     /locus_tag="WHT44_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ2533347.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(975..2168)
                     /locus_tag="WHT44_001161"
     CDS             complement(975..2168)
                     /locus_tag="WHT44_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ2533348.1"
                     /translation="MSDQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="WHT44_001162"
     CDS             complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="WHT44_001162"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ2533349.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGQVKAVDGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3021..3950)
                     /locus_tag="WHT44_001163"
     CDS             complement(3021..3950)
                     /locus_tag="WHT44_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ2533350.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKL
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDYLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="WHT44_001164"
     CDS             complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="WHT44_001164"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ2533351.1"
                     /translation="MRIEITPISESALVYRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4778..6985
                     /gene="uvrD"
                     /locus_tag="WHT44_001165"
     CDS             4778..6985
                     /gene="uvrD"
                     /locus_tag="WHT44_001165"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ2533352.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTRRMIECYAEYDKICQREGVVDFAELMLRSYEMLQNNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLIAGNHAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FILDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVVAFVRLIEALRNQVGQLSLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFEILPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKPAYDSYGNTRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEEM"
     gene            7509..8048
                     /locus_tag="WHT44_001166"
     CDS             7509..8048
                     /locus_tag="WHT44_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ2533353.1"
                     /translation="MSQVFKDFDLSSVWKTNSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAENHVQICEVSGIGFEKEGSLCGAMGQK
                     LWLEEWEYPPIGVYFANDPSGGHAMFALDYRACGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(8289..8450)
                     /locus_tag="WHT44_001167"
     CDS             complement(8289..8450)
                     /locus_tag="WHT44_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533354.1"
                     /translation="MNIINYKGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNPKLV"
     gene            complement(8458..8661)
                     /locus_tag="WHT44_001168"
     CDS             complement(8458..8661)
                     /locus_tag="WHT44_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533355.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(8574..8933)
                     /locus_tag="WHT44_001169"
     CDS             complement(8574..8933)
                     /locus_tag="WHT44_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ2533356.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
BASE COUNT         2243 a         2464 c         2216 g         2028 t
ORIGIN      
        1 cctctttcag ttacagcagc ttgatccctt tcccttatcc aacgggggaa ggctaggata
       61 gggtggcttg caaatataca gaacaaggga caagagccac cctctctcca accctctccc
      121 tccgtacggg agggggtgga ttctcgcggg cgaagcccac gctacggtta gcctttaccc
      181 cagcacaaac aattcccgcc cgtgcgcctt cagccaactt ttagcattgt cggtatgcgg
      241 cgtcagcgtg ttcaccaaat gccaaaagcg cggactgtgg tcggggtggc ggaggtggca
      301 gagttcgtgg atgcagacat agtcggcgac gtattcgggc gtgccgatca gccgccagtt
      361 gaggcggatg ccggtgtgcg ggcggcatac gccccaaaag gttttggcgt tgctcaggtc
      421 tgtggcggtg ggcgtcagtc ctgtttcggc tgcgtgtttt tcaaggcggg gcagcaggta
      481 ttcgcgggcg cgttcgttca acaggcggcg caggtggtcg atttgtgcgg cggtttcttt
      541 tcggggaagc aggatttcag acgacgtgat acggatatgg ctttggctgt gggtatccag
      601 cttggtcttt attccccgat accaaatcca ctcgggtaag tttgggtggg aaacaggatg
      661 cacgggcgtt ttggcaagcg tgttccgcaa aatcgtttcg tttgccgcca gccagtttgc
      721 taacgcgtgg tcttgaaaaa agggtgggac gttgatgctg accgtctgca tattgacggg
      781 gcgcagaatc agatttttct tggcactgcg tttgagttcg atttcgatgc acaaaccgtc
      841 ggaaagagta taggtgaagc gtttcatagt tgtgaatagg tttcagaccg gatacatcgt
      901 ctgaaacagg aattttccat atcaggcggc aaacttcgga taatatacaa aatcaaacat
      961 ctgcgctaca aggttcagcc gaacaagccg ccgatatatt tgctgatggt gatggcgctg
     1021 agtactgcca tcaaaccgac cacaatcacg ccggaaacgg tgagccacag cgggtgtttg
     1081 tagtcgccga caattttggt tttgtaggcg gcaatcagaa tcagaccgag ggaaatcggt
     1141 aaaatcaggc cgtttaatgc gcctacgaac accagcacct gcgccggttt gccgatggtg
     1201 gaaaatacgg cggtggacac ggcgataaag gcaataatcc atttgttttt attgcgttcg
     1261 atagacgggc tgagaccgga gaagaacgac accgaagtat aagccgcacc aatcaccgaa
     1321 gtaatcgaag ccgcccaaat caccacgccg aaaatcagca ggccgatgta tcccgccgca
     1381 tattcaaacg gtgtggaagc agggttgtcg ggattgagct gtacgccttg gctgaccacg
     1441 cccaaaaccg ccaaaaacaa tacaatccgc ataatcgagg caatcaggat cgcccgcacc
     1501 gagctttggc tcacttccgg caacgccgat ttgcctttga tacctgcgtc cagcagacgg
     1561 tgcgcaccgg cgaaggtgat gtagccgccg accgtgccgc ccaccagtgt aacaatcgcc
     1621 attgcatcga gtttttccgg cataaaggta tgcacggcgg catctgccag cggcggattc
     1681 gcctgccatg ccacataaac cgtcagcgca atcattacga aacccatcac ttgggcgaat
     1741 ttgtccatca ctttgcctgc ttctttaaac agaaacacac cgatggcaat cacgccgctg
     1801 atcacggcac cggtttccgg tgacagtccg gtcagcaggt tcagacccaa gcctgcgccg
     1861 ccgacgttgc caatattgaa cgccaaaccg cccatcacaa tcagcacagc caagaaatag
     1921 cctgcgccgg gcaagacctg attggcaata tcctgcgcct gtttttcgga aacggcgaca
     1981 atccgccaaa tattgagctg cgccccgatg tcgagcagaa tcgagagcag aatcacaaag
     2041 ccgaaacttg ccgccagtgc ttgggtgaag gtggcggttt gggtcagaaa gcccgggccg
     2101 atggcggaag tcgccatcag gaatgcagcg ccgattaagg catttctgcg gtttttttga
     2161 tcagacataa tcgcttatcc tctataaaat tggttgttgc tgtgtttggg cgaaacctgc
     2221 ggttttagct acgcagaaac tcgctttgct cgttttggcg aaacctgcgg ttttcagacg
     2281 gcctatgaac tgtttttcaa gcagaaactt tgatgcctgc cgccagtagt tcctgccgga
     2341 ttttttcggc aaacaccacg gcgtgcggcc cgtctccgtg cagacagatg ctgtcggctt
     2401 gcacggcaac caggctgccg tccactgctt tgacctgccc gtcccgcacc atctgcaata
     2461 cttgggcgat ggcttcttcg tcgctgtcca cctgcgcatc ggggcggctg cggggaacca
     2521 gcgtaccgtc gggcatatag cggcggtcgg cgaatacttc ggaaatcaca cccaagcctg
     2581 cggcttttcc ggcttccaag agcaggctgc cggaaagtgc catcaatttc aatttcgggt
     2641 cgaaatccgc cacaattcgg gcaacggtat ccgccagcgc acggtttttc gccgcttgat
     2701 tgtacattgc gccgtgcggt ttgacataag ccatttccaa accctgatca cggcacaagg
     2761 cctgcaatgc gcccaactgg taattcagac acgcccgcaa atcggcttcg gacagattca
     2821 tttcggtacg gccgaagttt tcccgatcgg gatagccggg gtgtgctccg atgcgcacgc
     2881 cgttttgttg ggcatacgcc aatgccgccc gaatatcggc aatgctgccg gcgtgttggg
     2941 cgcaggcgat gttggccgaa gtaatcagct gcaacaaggc ttcgtcgctg ccgcagcctt
     3001 cggcgagatc ggcgtttaaa tcaacctgct tcatgggtga ttctccgtat ttggttcaga
     3061 taggcttggt ttttgcgccg cagggcggtg gcttctttca agccgattat tttgaatttg
     3121 actttgctgc cgaagcgcac ctgtgccagc ctgcccaaat cggcggcggc aacggtagcg
     3181 attttcggat aaccgccggt ggtttgcgca tcggccagca ggataatcgg tttgccgccg
     3241 ggcggcacct gcacggttcc tgcctgaaca gcgtgggaca gcatttccaa aggttgcgac
     3301 agggtcagcg gctgtccgtc gaagcggtag cccatgcggt tgctatcgct ttgcagcgtc
     3361 cacgtttccc gttccagatt cagacgccct ttttcactga aagcggcata ttccgacgaa
     3421 ggaacaaggt ggacggtatc ggtaaacggt atcggggcaa tgccgacttt ggacaattcc
     3481 tgcgcacctt tgccgatggg gagataatcg cctttttgca gcattctgcc ctgatggccg
     3541 ccgaaaccgg ctttcaggtc ggtgcttctc gaacccatca cttccggcac atcaaatccg
     3601 cccgccacgc acacatagcc gtacatgccc tgcacggcac gcaccagttt caaggtctgc
     3661 cctttgcggg cggtataacg ccaatacgaa tagaccggtt cgccgtccaa ttccgcctga
     3721 tacacggcac cggtgagaca aaacggcgta tcccgttcaa acaccagcat tatcccgccc
     3781 aaagcgattt cgattgcggc cgtgccttcg tcgttgccca ataaaatatt gcccgccgcc
     3841 aaagcaaccg tgtccatcgc accggcatga ccgatgccgt aacgccggtg tccgtagcgt
     3901 ccggtatcct gaatatgcgc cggtgcctgc actgccgaaa cgtgaatcat ggctcaatcc
     3961 tttctgcaac aaagcggact tggtcacccg ccgccagcag ggtcggcgga ttcaaatcgg
     4021 ctcggaacaa gggtaattcg gttctgccga taatctgcca gccgccgggc gaagcgaacg
     4081 gatacacacc ggtctgactg ccgccgatac cgaccgaacc ggcaggaacg gacgttctcg
     4141 gcacggcacg gcggggcgtg tgcaatgctt cgggcaagcc gcccagataa gggaaaccgg
     4201 gctggaagcc catcataaat acggtataag tttgcgccgt atggcggcgg acgatttcgg
     4261 aaataaccgt ctgatggaaa gcagcgactt ccgccaaatc cgggccgtat tcgccgccgt
     4321 agcagacggg aatttccacc agtttgccct gatggtctgt aacggcggtg tgttcccaca
     4381 catattgcaa ttcatcggca agcgtcgcca aatcggtatc gaaacgggta aacacggtca
     4441 gattgttcat gccgaccacc acttcctcaa tcctgtcgtg ctgcccgagc gcagcggcaa
     4501 acgcccacaa cttttgctgt ttgcccagtt cggaaggcgc attcagtcgg tagaccaaag
     4561 cggattcgct gattggtgtg atctctattc tcatttgttg ttcattttgg ttatgtttta
     4621 atgaatctat atgcaggggc ggcggtttgt caatatcttc tgtgctgcat catcaaaccg
     4681 tcgattggaa aagtgctgcc ctgccgctgc actttttcag acgaccttaa accgtttcta
     4741 ttaaaatagc gcattccact tttcagacgg catccttatg tttcccgacc aatccgcccc
     4801 caacctgctg caaggcttga atcccgaaca actctccgcc gtaacctggc cgccgcaatc
     4861 cgcacttgtg ctggcgggcg cgggcagcgg caaaacgcgc gtgctgacca cgcgcatcgc
     4921 atggctgttg caaagcggac aagccagcgt gcacagcatt atggcggtaa cgtttaccaa
     4981 caaagccgcc aaagaaatgc aaacccgttt gggcgcgatg attcccatca atgtccgcgc
     5041 catgtggctc ggcacgttcc acggtctctg ccaccgcttt ttgcgcctgc accaccgcga
     5101 cgccggtctg ccgtcttcct ttcaaatcct cgacggcggc gaccagcttt ccctcatcaa
     5161 acgcctgctc aaaagcctca acatcgccga agaaatcatc gcgccgcgtt cgctgcaagg
     5221 ctttatcaac gcgcaaaaag aatccggttt gcgcgcttcc gtgttgagcg cgcccgatcc
     5281 gcacacacgc cgcatgattg agtgctacgc cgaatacgac aaaatctgcc aacgcgaagg
     5341 cgtggtcgat tttgccgaac tcatgctccg cagctacgaa atgctgcaaa acaacgaaat
     5401 cctgcgccag cactaccaaa accgcttcaa ccacattctc gttgacgagt tccaagacac
     5461 caacaaactg caatatgctt ggctgaaact gattgccggc aaccacgcag cagtatttgc
     5521 cgtcggcgac gacgaccaaa gcatttaccg tttccgtggc gcaaacgtcg gcaacatgac
     5581 cgcgctgatg gaagaattcc acatcgacgc gcccgtcaaa ctcgaacaaa actaccgctc
     5641 cgtcggcaac atccttgccg ccgccaatgc cgtgattgaa aacaacgacg aacgactcgg
     5701 caaaaacctg cgcaccgacg ccgaagcagg cgacaaaatc cgctactact ccgcctttac
     5761 cgacctcgaa gaagcccggt tcatcttgga cgaaaccaaa gccctcgaac gcgaaggctg
     5821 ggatttggac gaaatcgccg tcctctaccg tagcaacgcc caatcccgcg ttatcgaaca
     5881 aagcctgttc cgcagcggca ttccctacaa aatctacggc ggcttgcgtt tttacgaacg
     5941 ccaagaaatc aaacacgcgc tcgcctacct gcgcctcgcc gtcaatcccg acgacgacaa
     6001 cgccctcttg cgtgtcatca acttcccacc gcgcggcatc ggtgcacgta ccgtcgaaaa
     6061 tcttcagacg gcctcaaacg aacaaggcat caccctctgg caagccgcct gcaacgccgg
     6121 cgcgaaagcc gccaaagtcg tcgccttcgt ccgcctgatt gaagccctgc gcaaccaagt
     6181 cggacaactg tccctgtccg aaatcatcgt cggcatcctc aaagacagtg gcttgaccga
     6241 acactaccgc acccaaaaag gcgacaacca agaccgtctc gacaaccttg acgaactcgt
     6301 caacgccgcc atcgaattca aacccgaaga cagcaacttc gaaatcctgc ctgaaaacat
     6361 ttcagacgac cccgccttcc ccattctcgc cttcctaagc aatgccgccc tcgaatccgg
     6421 tgaaaaccag gcaggcgcag gcgaaaaggc cgtccaactc atgaccgtcc acgccgccaa
     6481 aggcttggaa tttaacgccg tcttcctcac cggcatggaa gaaggccgct tccccagcga
     6541 aatgagcctt gccgaacgcg gcggcctcga agaagaacgc cgcctcatgt acgtcgccat
     6601 cacccgcgcc cgcaaacgcc tctacatcac catggcgcaa caacgcatgc tgcacggaca
     6661 aacccaattc ggcatcgtct cccgcttcgt cgaagagatc ccacccgaag tattgcacta
     6721 cctgtccgtc aaaaagcctg cctacgacag ttacggcaac acgcgccaaa ccgccgcatc
     6781 caaagataaa atcatcgacg actacaaaca gccccaaacc tacgcaggtt tccgtatcgg
     6841 acaaaacgtc cgccacgcca aattcggcac cggcgtgatt atcgatgccg cagataaagg
     6901 cgaatccgcc cgactgacca tcaatttcgg caaacagggc gtgaaagagt tggacaccaa
     6961 gtttgcgaaa ttggaagaga tgtaaatttg aaatgtaggt cggatattcg tatccgacct
     7021 acggcaaaaa ccttagcagg agagaataga aacccgtagc gtgggctttt tctatgaatc
     7081 aagcccaaaa tttcagacgg catttttagc cgtcattatc gtggatgaag cccacgctac
     7141 aatgtacaca cagagcaaat agagatgtgg gtcggatatt cgtatccgac aaaaacattt
     7201 gacgcgtcta ttgtttccga aacaccgctg ttggaaatgt cggatacaag aatctgactt
     7261 acggcaaaaa acgtagtaag gacaaagcaa aaggccgtct gaaaacggga agggcaattt
     7321 tgccgcaacc gccgccgtca ttcccgcgca ggcgggaatc cagacctttc ggcacggaaa
     7381 cttatcggat aaaaggtttc tttagattcc acgtcctaga ttcccgccgg aacataaatg
     7441 acggacggta aaagccgggt atgaataccc accctctgtt atcactgaga tcaataagga
     7501 agaacattat gtcccaagtt tttaaagatt ttgacttgtc ctccgtatgg aaaactaata
     7561 gttgggcaga tgaaaactac aaagaagccc cgtttacccc tgaaattttg gctgccgtag
     7621 aaagtgaact gggctataaa ttgccgcaaa gttttattga attgatggca gtacaaaacg
     7681 gcggaatatt tgtcaaaaac tgttttccga ccacgcagag aaattcgtgg gcggaaaatc
     7741 atgtgcaaat ttgcgaggta tcgggaatcg gttttgaaaa agaagggagt ttgtgcggcg
     7801 cgatggggca aaaactttgg ctggaagaat gggaataccc gcctatcggc gtgtattttg
     7861 ccaacgaccc gtcaggcggt catgccatgt ttgccttaga ctatcgggcg tgcggcaaag
     7921 acggcgagcc gaaagtggtg tttgtcgaac aagaatcgga ttttgaaatc gtcgaacttg
     7981 cccccgattt tgaaaccttt atccgcagct tgcggcatga agatgagttt attgacgaag
     8041 aaatataaaa cggtggttga aaaactgaaa tcatcaagag aaaacgggcg aaataacggg
     8101 taatcgcttg aatccgtaag gaaaacggtt tggtggaacg cgccatccaa gacctttgca
     8161 aaaaactgtc cccgacagca ttgacattat taacagaact tatcaatttt ggagctatct
     8221 caaatataat tcggttatcc tgttgtatcc attaaatcat atgcttcaat taattgttgt
     8281 tctagctctt ataccaattt tggattgcga attcctgaca caatctcaaa ttcttctgca
     8341 tctatgcaaa cacctgcata aatttcaata acaagggaac gcaataattg aagctcttct
     8401 cttgttaaag aaataataat gtcatcacct ttgtaattga ttatattcat aataatttta
     8461 tttttgtttg tcaaagtaag ttttgcctaa ggttggtcta aatgcagttc caccatcttt
     8521 tgaatttggg tctctgatta caattgctcc agacttatca tcccaaattg ctcttatgtg
     8581 tttggattgt aatcttcgaa ttcccaagaa aaaaatcgta ataagtttga aagtgtcaaa
     8641 tcccaagttt cttttgagca atattctaat attttatcaa tttcactttt aataatctta
     8701 tgatcaaact gttctaatat taatgcatta gaccaaaaaa aaccttcttt attacaatga
     8761 tgggaaatcc atttaggaga acaaatgcaa agtgaaaaaa tagatgagcc ttgttctcct
     8821 tcgattccga tatccaaatc tatccatcta tggaaattat ctggaatttc gggggtaaat
     8881 ttttcaaaat caatatcata taaatttatg ctttttaaat ccaatttaat cattagggct
     8941 gtcctagata a
//
LOCUS       DAWGRZ010000071         8929 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000071 DAWGRZ010000000
VERSION     DAWGRZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8929
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.071"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..177)
                     /locus_tag="WHT44_001170"
     CDS             complement(<1..177)
                     /locus_tag="WHT44_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ2533357.1"
                     /translation="MSKIICLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNDY
                     GLTVRTDGEGKGNMP"
     gene            complement(320..841)
                     /locus_tag="WHT44_001171"
     CDS             complement(320..841)
                     /locus_tag="WHT44_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533358.1"
                     /translation="MECHADVWHFILEKNMKKFYFVLLALGLAACGQEQSQKADAEQY
                     FFANKYQFADEKQAFYFERAARFRVLQQGLGGDFERFLKGEIPNQENLAKYRENITQA
                     VAYYADTNGDDDPYRVCKQAAQDAEILMKSMVTSGGGGTTDLDKESYQNYRKSMQECR
                     KTITEAEANLPKK"
     gene            complement(843..2015)
                     /locus_tag="WHT44_001172"
     CDS             complement(843..2015)
                     /locus_tag="WHT44_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ2533359.1"
                     /translation="MKQINQALQQKLVEFKEKSGMNQTQLARGIGTSPASISMYLNGT
                     YAEKGGNYETIEPKIEAFLEMQDSKAQREELVLGFVSTKTTRRIAEVMRDAHEGGETV
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIVRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSVALNLGESLPDDELFEIAKAALPDAVKHLLPDSVQALITVIGFNETLE
                     LVRLMGGTTYPLRQGYTKNSQSRVAYLEEIIGSEAAGRLVEAMAPCNLFIPRCETALY
                     ELRNRKIRSQFDRQTAGGTPAYEAVNDLALAHRLSDRHVWRILKQADKEAEQENLF"
     gene            2061..2318
                     /locus_tag="WHT44_001173"
     CDS             2061..2318
                     /locus_tag="WHT44_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ2533360.1"
                     /translation="MKEFPDLEKRLIMLEVAVQDLEDRSLADSFVLSWLLQRITRQEP
                     TSIEQVRSFLQAQAKTFEPDSVQREHLESLLELVESAQELA"
     gene            complement(2385..4370)
                     /locus_tag="WHT44_001174"
     CDS             complement(2385..4370)
                     /locus_tag="WHT44_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ2533361.1"
                     /translation="MKISASDIAKLGIPSLPTDRQGIEYHAKKNNWQHCFEQKGRGRP
                     KKLYEIASLPAEIRAAIMKRQSDELAEKMPKMLPKVRPGTAMSAQALAEAAKLLNEKQ
                     RSVADARCAVVAAVLGIKYEYDCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSRT
                     AKVGERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSAP
                     KLAHSYRWFVQWAEAENMPVNDVPNLSMVRRVWEKLPLIMQERGRKTGAAYKSLLPYV
                     KRDWGALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAES
                     CVAVSDAMRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRPGIRHETGIAGNPQ
                     GRGIIERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQ
                     KYLKKLPSWSRFIADVVKCIDEYNNRPHGELPRHPDGGHYTPKAYREMRLEQDGIAPD
                     MLSAQELATMFMPQEVRKVQRGWLDLFNNSYFSTELAEYHKDEVRVSYDLSDASAVNV
                     FDMDGKFITKAQANGNTREAFPTARIDQLAEKRRKGKIKRAENAIKLANAEVNPALEQ
                     AAVWDELGHLGGNDIEAEYAVLPKTGTDDFVLFEADR"
     gene            complement(4432..4674)
                     /locus_tag="WHT44_001175"
     CDS             complement(4432..4674)
                     /locus_tag="WHT44_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEZ2533362.1"
                     /translation="MTDWHRADIVARLKKAGWSVRALSIEANLAPNTLGKALDAPYLK
                     GERIIAAAIGVPAEEIWPSRFEKRNHKPTFPRSINR"
     gene            5184..5624
                     /locus_tag="WHT44_001176"
     CDS             5184..5624
                     /locus_tag="WHT44_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ2533363.1"
                     /translation="MGNPVDLSEFVFVPRYNVAAAAGYGAPVFGEEPLFCLAFRKYWI
                     DNYVTKQPEKLSVITVKGDSMEGVLNHEDSILINHAETDPRDGLYVLRIGNDLFVKRV
                     QRIPGKLLVTSENPRYAPFEIDLSNAQDDIAIIGRVEWYGRSID"
     gene            5873..6424
                     /locus_tag="WHT44_001177"
     CDS             5873..6424
                     /locus_tag="WHT44_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ2533364.1"
                     /translation="MSGININLKNGDSLLIRGPSGCGKTSLLRALAGLWPFGSSGKVS
                     RPPHQDILFLPQRPYTAQGSLRDAVCYPDIDKQHPELAEAMNTCRLGYLVDKLDKTDD
                     WQHKLSPGELQRVAFVRALLSKPKIVLLDEATAALDEPTEALLYRALKQKLPDSIIIS
                     IGHRSTLNAFHNMRLDVGNVACG"
     gene            6529..7197
                     /gene="hda"
                     /locus_tag="WHT44_001178"
     CDS             6529..7197
                     /gene="hda"
                     /locus_tag="WHT44_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ2533365.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            7194..7862
                     /locus_tag="WHT44_001179"
     CDS             7194..7862
                     /locus_tag="WHT44_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ2533366.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCHLFGITNIIGTQLETGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLQSYGKTYFYSDSKNDLPLLRLVSEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            8218..8325
                     /locus_tag="WHT44_001180"
                     /pseudo
     CDS             8218..8325
                     /locus_tag="WHT44_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         2279 a         2456 c         1845 g         2349 t
ORIGIN      
        1 gggcatattg cctttgcctt cgccgtcggt gcgtacggtc aggccgtaat cgttacgcag
       61 gattgaagcc acaatgttgc gcatatcctg cgccaagtcc gcctcacggt cgctgccgtt
      121 gactgcgccc gggtcggtgt tactgtgtcc ggcagtcaga caaataattt tgctcattaa
      181 agcctccctc aaaatcagat taaaatgcac tttcagaggc ttacattttc aaacggcatg
      241 gcttttgcag cgggcgaaac agtgtcagta ggcaacaaaa aagccgcctg ttgttcagac
      301 ggccttagaa tcgtttattt tattttttcg gcaaattggc ttcagcttcc gttattgttt
      361 tacggcattc ttgcattgat tttcggtaat tttgataact ttccttatct aaatcagttg
      421 taccgcctcc accgcttgtt accatactct tcatcaggat ttctgcatct tgcgcagcct
      481 gtttgcagac gcggtatggg tcatcatctc cattcgtgtc cgcataataa gcgactgctt
      541 gagtaatatt ttcacgatac tttgcaagat tttcttgatt aggtatttct ccttttaaaa
      601 acctctcaaa atccccgcca aggccttgtt gcaatacacg gaaacgggcg gcgcgttcaa
      661 aataaaaagc ctgtttctca tctgcaaatt gatatttatt ggcaaaaaaa tactgctccg
      721 catcagcttt ctgcgattgt tcttgcccac acgctgccaa acccaacgcc agcagcacaa
      781 aataaaactt tttcatattt ttctccaaaa taaaatgcca tacatctgca tggcattcca
      841 ttctaaaaca aattctcctg ctccgcttcc ttatccgcct gctttaaaat tcgccacaca
      901 tggcggtcgc ttaggcggtg tgccaaggcc aaatcgttaa cggcctcata agcaggggta
      961 ccgcctgccg tctgccggtc aaactgactg cggattttac ggtttcgcaa ctcatacaag
     1021 gccgtctcgc aacggggtat aaacagattg cacggagcca ttgcctccac cagccgaccg
     1081 gccgcctcac tgccgataat ctcttccaag tatgcaacac gggattgact gtttttcgta
     1141 taaccctgcc gcaaaggata agtcgtaccg cccatcaggc gcaccagctc cagcgtttca
     1201 ttaaacccga tgaccgtaat caacgcttgt acactatcag ggagcagatg cttgaccgca
     1261 tcaggcaaag ccgctttcgc aatctcaaag agttcgtcat ccggcaaaga ttcgcccaaa
     1321 ttcagcgcaa cagacacgcg gctgtaaagc tgtaccagtt cgccatgctt accgcgcagg
     1381 ttggccacca gtcggggcat accgctcaac accaagccgc agccagtctc gtcgtgcaga
     1441 cggcgtacaa tttcaagggc gcgtaaaggc aggttttccg cttcatcgac cacaatcaga
     1501 cggcccgaat cgcgcaggcg gtcagataca gactcaaaca aatcattcag gctgcccatc
     1561 gccgatacct tcgccgcagt cgccaacttg cgcatcaaga ccaaagccgt aaagctcgga
     1621 ttagcctcaa tcaagatggc cgcagggttt ttctcgcagt agtttttgac cgcctgagtc
     1681 ttgcccaatc ccgcctgacc gtagatcacc actgtttcgc cgccttcgtg cgcatcgcgc
     1741 atcacttctg caatacggcg ggtcgtctta gtcgatacaa aacccaacac cagctcttcg
     1801 cgttgcgctt tactgtcctg catctccaaa aacgcctcga ttttcggctc gatggtttca
     1861 taattgccgc ctttttccgc ataagtgccg ttcagataca tactgatgga tgccggcgaa
     1921 gtaccgatac cgcgtgccag ttgggtttgg ttcatgcctg atttttcttt aaattcaacc
     1981 agtttttgtt gcaatgcttg attaatttgt ttcattttta atatccttga gttttaaaca
     2041 acctttaaag gccgtctgaa atgaaagagt ttcccgattt agaaaaacgc ctgatcatgc
     2101 tggaagtcgc cgttcaagac ttggaagaca ggtcacttgc cgattctttc gtactttcct
     2161 ggctgctcca gcggattacc cgtcaagagc cgacttcgat tgagcaggtt cgcagtttcc
     2221 ttcaagcgca ggcaaaaacg tttgagcctg attccgttca gcgggaacac cttgaatctt
     2281 tgcttgagct cgttgaatcc gcccaagagc tcgcttgagt ttcaattttt caacaagctc
     2341 ggccgaaaac gctgcatttt gctgtttgtc catcatgttt tcctttatct atccgcctca
     2401 aacaacacaa aatcgtctgt gcccgttttc ggcaataccg catactccgc ctcgatgtcg
     2461 tttccgccca aatgtcccag ctcgtcccaa accgcagcct gttccagagc aggattgact
     2521 tccgcgtttg cgagcttgat tgcattttcc gcccgcttta ttttgccttt tcgacgtttt
     2581 tccgccagtt ggtcgatacg agccgtcggg aaagcctcgc gggtattgcc gttggcctgc
     2641 gccttagtaa taaacttgcc gtccatatca aacacattga ccgccgacgc atcgctcaaa
     2701 tcgtagctga cccgtacctc gtctttgtga tactccgcca gctcggttga gaaataagag
     2761 ttgttaaaca aatccagcca accgcgctgt acctttcgca cctcttgcgg cataaacatc
     2821 gtcgccagct cttgcgccga caacatatcc ggcgcgatac cgtcctgttc cagcctcatt
     2881 tcccgataag ccttaggcgt ataatgcccg ccgtcaggat gtcggggcag ctcgccgtgc
     2941 gggcggttgt tgtattcgtc gatacacttg accacatccg ctataaaacg cgaccagctc
     3001 ggcagttttt tcaaatattt ctgttgttcc tccgtcaaat ccttgccttt ttccaaagcg
     3061 ttaaacgcac tttccatctt gcggtacatc aggttcttcg tgctgctgtc catccctgca
     3121 cccgcaaacg tctcatactg gcgcgccatc tcaatcagat tgtctttcca ccatcgctca
     3181 atgatgccgc gcccttgcgg gttgcccgcg atacccgttt catggcggat acccggtcgg
     3241 gacgtaatac ccgtgatttc atggtctatc gtcttgccgg tttggccgcc gccgttatcc
     3301 gagtaataga taatcggcaa accaaaatgc ttgaccccga tacgcatagc gtccgatacc
     3361 gccacacaac tttcagcaag agagaccgaa aacccgacca caaaccgcgt acaaccatca
     3421 ataatcaccg tcacttccgg cttaaatggt ctgccatgta ccggatgcgc cacctttgcc
     3481 ttaaagctat ggccgtcgcc gatccaaaca tcgttcggct tcaaagcccc ccaatcacgt
     3541 ttcacataag gcagcagcga tttataagcc gcccccgttt tcctgccgcg ctcctgcata
     3601 atcaacggga gcttttccca aacgcgccgc accatactca agttaggcac atcattgacc
     3661 ggcatatttt ccgcttccgc ccactgcaca aaccagcggt agctgtgcgc cagctttggc
     3721 gcggacggaa tattgtgaaa ctgcataaac atcggcaacc atccgtaact ttcaatcggc
     3781 ttgaccgcct tcgtcgtctt cggagccaaa gcaaccaacc gctccgtcgc gttttccgct
     3841 ttcaaataag cagaaatcca gccgtctaaa gtacgttcgc cgaccttcgc cgtccggctg
     3901 cggtcattgg ccttttccaa gttcccaagc gtgaccgcgt ccaatttacc ttctgccagc
     3961 aagcccaaaa actgagccac cgcagccttg gcagagcaat cgtattcgta tttaatcccc
     4021 aataccgccg ctaccaccgc acatcgcgca tccgccaccg accgttgttt ctcgttcaac
     4081 agcttggccg cttcagccag tgcttgagcc gacatcgccg tccccggtct gactttgggc
     4141 agcattttcg gcatcttctc cgccagctcg tccgactgcc gtttcatgat ggctgctcgg
     4201 atttcggcag ggagggaagc gatttcatac agttttttag gacggcctct tcctttttgc
     4261 tcaaaacagt gttgccaatt atttttcttg gcatggtatt caatcccttg tctatcagtt
     4321 ggtaggctcg gaattcctaa tttcgcaata tcagatgcag atattttcat tttttccgct
     4381 ttctattttt gcttaaatgc ggtactcttt tggaactatt tagcaaaaca gttatctatt
     4441 tatagatctt gggaaggttg gcttatggtt tcgtttctca aaacgagatg gccagatttc
     4501 ttctgcgggt actccaatcg ctgctgcaat gattctttcg cctttcagat aaggagcatc
     4561 taaagctttc cctaatgtat ttggtgctaa attagcctcg atagataacg ctctcacaga
     4621 ccagcctgcc tttttaagac gagccacaat gtcagcacga tgccaatcag tcatgctttc
     4681 ttgctttttt tcattctcgc aattccttaa ttaagtaggt ttattaaccg ccgtcattta
     4741 gcaaatgagt gaattacatt tagcaaaaca gcttcatgaa actcttttgc taaaatattt
     4801 tctcaaaaac gtaattattt ttacaaaata ttgattattt gagtaatctt atttaagcaa
     4861 aagagtttct ttttttgctt aatgttttgc ttaaatgagt taaattagga ttactatgga
     4921 tactttttta gataggctca aatttctttg gcctcatggg gctaagccat ctgacatcta
     4981 taacaaaata gaaatgtcgg cttctggatt taatagagtt tggaaagagg gagctatccc
     5041 aacagccgat tacctagtaa aaattcagtt gtgatttaaa ctggctgctg accggaaaag
     5101 gcttgccgta tctcgataaa gccaggccgg aaaacgccgg agcattctct gtaagtagaa
     5161 attctgatgg aatcaccgac actatgggca atcctgtcga cctgtccgag tttgtattcg
     5221 tgccgcgcta caacgtagcc gcagcagcag ggtatggcgc accagtcttc ggcgaagaac
     5281 ctttattctg cttggctttc cggaaatatt ggattgacaa ctatgtgacc aaacagccgg
     5341 aaaaactgtc tgtaattacc gttaaaggcg attcaatgga gggcgtgctc aatcatgagg
     5401 attccattct catcaaccac gccgaaactg acccgcgtga cggcctatat gtcttgcgta
     5461 taggcaatga cctctttgtc aaacgtgtgc aacgtatacc gggcaaactg cttgtaacgt
     5521 ctgaaaatcc acgttacgca ccgtttgaaa tagatttgag caatgcccaa gacgacatcg
     5581 ccatcatagg ccgtgttgaa tggtatggcc ggagtattga ttaaatccat tttaaaaacc
     5641 tcttaaaaca gttttaaaaa ttccctaatc tcgtcagctt taaacagaaa accgcgcatt
     5701 ccggcgcggt tttgtgaaaa agctggcgta acttttccgg atacaaaaaa acgccgaaat
     5761 ccacgtcttc cgaagatttc agcgtttttt tactctattt gttccttgtg caaaaactaa
     5821 cagtccccca caggaaaacg tcgctctgtt ccgccacaac ggcgaaatcc tgttgagcgg
     5881 catcaacata aacctcaaaa acggcgattc cctgctgata cgcggcccga gcggttgcgg
     5941 caaaacctcg ctgttgcgcg cgctggcggg gctttggccg ttcggcagca gcggcaaagt
     6001 cagccgtccg ccgcatcaag acatcctctt cctgccgcaa cgcccgtaca cggcacaggg
     6061 cagcctgcgc gacgcggttt gttaccccga cattgacaag cagcatcccg agttagccga
     6121 agccatgaac acctgccgct tgggttatct ggttgataaa ttagataaaa ccgacgactg
     6181 gcaacacaaa ctctccccgg gcgaactgca acgcgtcgcc ttcgtccgcg ccctgctttc
     6241 caagcccaaa atcgtcctgc tcgacgaagc caccgccgct ttggacgaac cgaccgaggc
     6301 cctgctctac cgcgcactga aacaaaaact gcccgacagc atcatcatca gcatcggcca
     6361 ccgcagcacg ctcaacgcgt tccacaatat gcgcctcgat gtcggcaacg ttgcctgcgg
     6421 ttgaacgatg ccgtctgaag ccgttttcag acggcatcgt tcaaattgga aaggcgcgcg
     6481 aaaacaacgt acaataaacc cgtttaccac ccctcggaat gccgaaccgt gaaccagctt
     6541 attttcgact ttgccgcaca cgactatccg agtttcgaca aattcctcgg cacggaaaac
     6601 gcggaactgg tctatgtcct ccgacacaaa cacggacagt ttatctatgt ctggggtgaa
     6661 gaaggcgcgg gcaaaagcca tcttttgcag gcgtgggtcg cacaggcgct cgaagccggc
     6721 aaaaacgccg cctatatcga tgccgcctcc atgccgctga ccgatgccgc gttcgaggcg
     6781 gaatacctcg ccgtcgatca agtcgaaaaa ctgggcaacg aagaacaagc cctgcttttt
     6841 tccatcttca accgcttccg caacagcggc aaaggctttt tgctgctcgg ttcggaatac
     6901 acgccccagc agcttgtcat ccgcgaagat ttgcggacgc gtatggcgta ctgcctcgtt
     6961 tacgaagtca aacccttaac cgaccaagaa aaaatcgacg cgctcgccag tatggcggcg
     7021 gcacgccaag taaccgtcga ttccgaaatt ttcgaatacc tgctcaaaca ctggcggcgc
     7081 gatatggaca gcctgatgat gatgctcgac acgctggaca actacgccgt cacgatgggc
     7141 aaacgcatca ccctgccgct tttgcgccag cttttgaaac aacaggaaac ccaatgaaaa
     7201 acctcgccat cttcgacctc gacaacaccc tcatcaacac cgattccgac cacgcctggc
     7261 cgcaatacct catcaaaaaa ggcttggttg acgctgccga aaccgaagcg caaaacgaaa
     7321 aattctaccg cgactaccaa aacggctgcc tcgacatcga cgcattcctc aaattccacc
     7381 ttgccccgct cgctcgttac agcaaagaag agctggcaga gtttcaccgc gaatttatgg
     7441 cggaatacat catcccccac atctcgccta tgcagcgtat gctggtgcag agccaccaaa
     7501 tggcaggcga cgaaaccctt gtgatttcct caaccaacga gttcatcatc acccccgtct
     7561 gccacctttt cggcatcacc aacatcatcg gcacacaact cgaaaccggt tctgacggcc
     7621 gctacaccgg caattacatc ggcacgccca gcctcaaaga aggcaaaatc acccgcctga
     7681 accaatggct tgccgaacgc ggcgaaacgc tccaaagcta cggcaaaacc tatttttaca
     7741 gcgactccaa aaacgacctg ccgctgctgc gccttgtcag cgaacccgtc gccgtcaacc
     7801 ccgatgccga gctggaaaaa gaagccaaag aaaaaggctg gccggttttg aatttcaaat
     7861 gacgcaatgc cgtctgaagc ccgattcggc gttcagacgg cattgaggct gaaatccaaa
     7921 aaactcaaag ccacaggaat ttatccggaa aaacaaaaaa cacaggaatt catcgggaaa
     7981 aacaacaatc tttccaccgt cattcccacg aaagtgggaa tcttgatccg tccgcacgga
     8041 aacttatatt tcgtcatttg cacccgacat aattcaatac tttattttgc aataaattaa
     8101 cggatgctga caaattagat tggattaatt accctattat ccatattggg ttatcgggtt
     8161 gctaattgag cagagcgtag caaaccataa agtgagtgtt tccacccact ctgcatttat
     8221 agtggattaa atttaaatca ggacaaggcg acaaagccgc agacagtaca gatagtacgg
     8281 caaggcgagg caacgctgta ctggtttaaa tttaatccac tatactatga agtggttatt
     8341 aaatatgata atgagaccta ttaaatttag tatggtaaat acgttattat ttattgttat
     8401 atgtagttca ttttttgatc tgctcgttca attatgtaca attttatttc atagccaaaa
     8461 aatatacttt attacattat ttttattatt tatttttaat tttgttacaa aatctatcta
     8521 tatggcaatt atttatccta ttttatattt ttttacgata aaaaaatatt atccttactc
     8581 taggaaagtg ataattctat tatcattagc attatctata tattttagtt ttatggactt
     8641 ttactttttt tccatatatt cagataacct tagctatgaa acggagcctt tacatttata
     8701 catccctatt attattaatt ttttctcact tttagtttct aattttattt tatcttttat
     8761 caacaagtaa atgtatatac aaaatacaat ccaaaagatc ctaagcaaac caccaatccc
     8821 acaccaccct taccggttca aatccgcaca aaaacccaca tcccgtcatt cccacgaaag
     8881 tgggaatcta ggacgtatag tggattaaca aaaatcagga caaggcgac
//
LOCUS       DAWGRZ010000072         8911 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000072 DAWGRZ010000000
VERSION     DAWGRZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8911
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.072"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..164)
                     /locus_tag="WHT44_001181"
     CDS             complement(<1..164)
                     /locus_tag="WHT44_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEZ2533367.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIE"
     gene            403..1302
                     /gene="crgA"
                     /locus_tag="WHT44_001182"
     CDS             403..1302
                     /gene="crgA"
                     /locus_tag="WHT44_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ2533368.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLSVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIVCLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1527..4742)
                     /gene="carB"
                     /locus_tag="WHT44_001183"
     CDS             complement(1527..4742)
                     /gene="carB"
                     /locus_tag="WHT44_001183"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ2533369.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPANGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSEDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQVVHSAEELQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQVLGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4771..5412)
                     /locus_tag="WHT44_001184"
     CDS             complement(4771..5412)
                     /locus_tag="WHT44_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533370.1"
                     /translation="MSIPINFNNLKYLLNDMRNKNRIIEAFPFNYNQRQYAVILTRYK
                     PDEPRPDDYAQAKLEFFNLNSENSIFAYADFYEVHFKSATDFINFFKINVQAGAAKIR
                     EIFQSFSNLFADFIPTQTKKDLDIIYKKIVATRLEPNSPNTIYCYDVRRNGKDKAGKP
                     NRRSVENSEKAKILRPELYEKFKADSNYSFFFSDNPSDEKTDAEIIREVTNRQ"
     gene            complement(6094..6483)
                     /locus_tag="WHT44_001185"
     CDS             complement(6094..6483)
                     /locus_tag="WHT44_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ2533371.1"
                     /translation="MSTHEKLLTADNPVLHQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHAEQAVYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLAEILRVLQELEKQYAQ"
     gene            complement(6924..7217)
                     /locus_tag="WHT44_001186"
     CDS             complement(6924..7217)
                     /locus_tag="WHT44_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEZ2533372.1"
                     /translation="MEEVSFGSDRKALLFGSQKINLHGRGAEIQPNAQHAACGTADLC
                     LLTDTPLETVLQELSAHGIKPLSGIVARTGAMGKIQSVYLRDPDGNLLEISSY"
     gene            complement(7458..8591)
                     /gene="carA"
                     /locus_tag="WHT44_001187"
     CDS             complement(7458..8591)
                     /gene="carA"
                     /locus_tag="WHT44_001187"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ2533373.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWALGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAEDVLALNPDGVFLSNGPGDPEPCTYAIKAVQKLIESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         1993 a         2265 c         2356 g         2297 t
ORIGIN      
        1 ttttcgattt ctgcctcaac atcatagccg gcatcaatca cggtttcttg aacattgtgt
       61 ccgagcgcgg tcaaaacttc tttcgctttt ttgtgaagcg tacggtttaa cccgccgtga
      121 gaatgtccga acgccttgcc gccgtctaat aataaaatat tcatttgtta cctcgtttgt
      181 gaattgatga atgtattgtg ccgttttact ttataattta aaagtgcaaa aataagaaaa
      241 cacttttgcg tcaaatgaaa taatcagatg ttccaacaca cgggatggca cataaagcac
      301 cgccctgtgt gccgtcctga ttcggaaggg gttacacccc tcccaaataa agtctgatcc
      361 tgccgcccta aagggcgggg ttccaaccga aaaggaaaca cgatgaaaac caattcagaa
      421 gaactgaccg tatttgttca agtggtggaa agcggcagct tcagccgtgc ggcggagcag
      481 ttggcgatgg caaattctgc cgtaagccgc atcgtcaaac ggctggagga aaagttgggt
      541 gtgaacctgc tcaaccgcac cacgcggcaa ctcagtctga cggaagaagg cgcgcaatat
      601 ttccgccgcg cgcagagaat cctgcaagaa atggcagcgg cggaaaccga aatgctggca
      661 gtgcacgaaa taccgcaagg cgtgttgagc gtggattccg cgatgccgat ggtgctgcat
      721 ctgctggcgc cgctggcagc aaaattcaac gaacgctatc cgcatatccg actttcgctc
      781 gtttcttccg aaggctatat caatctgatt gaacgcaaag tcgatattgc cttacgggcc
      841 ggagaattgg acgattccgg gctgcgtgca cgccatctgt ttgacagccg cttccgcgta
      901 atcgccagtc ctgaatacct ggcaaaacac ggcacgccgc aatctacaga agagcttgcc
      961 ggccaccaat gtttaggctt caccgaaccc ggttctctaa atacatgggc ggttttagat
     1021 gcgcagggaa atccctataa gatttcaccg cactttaccg ccagcagcgg tgaaatctta
     1081 cgctcgttgt gcctttcagg ttgcggtatt gtttgcttat cagatttttt ggttgacaac
     1141 gacatcgctg aaggaaagtt aattcccctg ctcgccgaac aaacctccga taaaacacac
     1201 ccctttaatg ctgtttatta cagcgataaa gccgtcaatc tccgcttacg cgtatttttg
     1261 gattttttag tggaggaact gggaaacaat ctctgtggat aattctataa aaacctgtca
     1321 taaaactgtt tttactcact taataacttt gagaaaaaat aaataagata atgtatagtc
     1381 ggcggcaatg gcaatctata tggggagcgt gatagggctg ttccttatgt acggcttgtg
     1441 tgtggcaaca tcggctttcc aaatgacaaa atgccgtctg aaaccgcaaa acggcttcag
     1501 acggcatttt caacctgatt caggcatcag ttgcggtttt tcaaacgccc gtgcagttct
     1561 tgaacgctgt acacgcccag atggtctcgg cttttcgcgc cttcgctcat cgcttcgccg
     1621 ccggcggtgg tggtgtattg cggcacacgt tgctgcaatg cgctttggcg gatgatgtgg
     1681 ctgtcggaca cggattgcgg atcgctggaa acggtgttca cgaccagtgc gatttcgccg
     1741 tttttcagcg cgtcgccgat gtgcgggcgg ccttcgggta ctttgttgat ggtctgcaca
     1801 atcagcccgt gttcggtcag gtattgcgcc gtgccgcgcg tggcgcagat gccgtagcct
     1861 aaaacttgga agtttttagc ggttttaatg acgcgttctt tgtcttcttc gcgcacggag
     1921 aggaagattt tgccggtcgg gttgaggcgt tcgcccgcgc cgagttgggc tttgtagtag
     1981 gcttcgccaa agcttgcgcc cacgcccatg acttcgccgg tggagcgcat ttccggtccc
     2041 aaaatcgtat ccacgcccgg gaatttgatg aatgggaaca cggcttcttt aacggcatag
     2101 aaatcgggga caacttcttt ttccacgcct tgttctttca gggaaatgcc tgccatgcag
     2161 cgcgcgccga ctttggcgag cggcacgccg gtggctttgg agacgaaggg cacggtgcgg
     2221 ctggcgcgcg ggttcacttc caatacgaac actacgccgt cttgtacggc aaactgcacg
     2281 ttcatcagtc cgaccacgcc cagcgcgtac gccatcgctt tggtttggcg gcggatttcg
     2341 tcttggattt cttcgcttaa ggagtagggc ggcagcgagc agccggagtc gccggagtgg
     2401 atgcccgcct gttcgacgtg ctgcatgatg ccgccgataa ccacgtcttt gccgtctgaa
     2461 acgcagtcca catccacttc aatcgcgttg ttcaggaaga agtcgagcaa cacggggctg
     2521 tcttcggaaa cctgcacggc ttcgcgcatg tatttttgca gctcttcggc ggaatggacg
     2581 acctgcatgg cgcggccgcc gaggacgtaa gacgggcgca cgaccagcgg atagccgatt
     2641 tcttcggctt tgacgagcgc ttcttcttcg ttgtgggcga tgcggttggg cggttggcgc
     2701 aggcctaagt cgttcaacac tttttggaag cgttcgcggt cttcggcggc gtcgatgctg
     2761 tcggcggacg tgccgatgat gttcacgccg ttttcaacca gcgcgttggc gagtttgagc
     2821 ggggtttggc cgccgtaatg cacaatcacg ccccacgggt tttcggtgcg gacgatttcc
     2881 aacacgtctt ccagcgtcag cggctcgaaa tacaggcggt cgctggtgtc gaagtcggtg
     2941 gacacagttt cggggttgca gttgaccatg atggtttcaa agcccgattc gcgcagggcg
     3001 agcgcggcgt gaacgcagca gtagtcaaac tcgatgccct gaccgatgcg gttcgggccg
     3061 ccaccgagaa tcatcacttt tttgcggtcg gaaggacgag attcgcattc ttcttcgtaa
     3121 gtggagtaaa gataggcggt ttcggtggcg aactcggcgg cgcaggtatc gacgcgtttg
     3181 taaaccggat gcagcttcag cgcgtagcgg tgttcgcgaa cttctttttc gcttacgttc
     3241 aacaattgtg ccaaacgttt gtcggagaag cctttgcgtt tcagacgacg tagggcggcg
     3301 aaatccaaat cactcaaaat gccgtctgaa accgcttttt cttccttcat caagtcttcg
     3361 atttgcgcca agaaccaagg gtcgatggcg cagatttcgt ggatttcttc cagcgtgaag
     3421 cccgcgcgga acgcgtctgc cacaaacagc atacgttcgg ggccggggtt cgccagttcg
     3481 cggcggattt ccgctttgtc ttcactgcgc ggattgaagc cgcacaagcc tgtttccaag
     3541 ccgcgcaggg ctttttggaa actttcttga atcgtgcggc ccatcgccat cacttcgccc
     3601 accgatttca tctgcgtggt caggcggtcg tctgcggcag ggaatttttc aaacgcgaaa
     3661 cgcgggattt tggtaaccac atagtcgatg gaaggctcga acgacgcggg ggttttgccg
     3721 ccggtgatgt cgttgcgcaa ctcgtccagc gtaaagccga ccgccagctt cgccgccacc
     3781 ttcgcaatcg ggaaacccgt tgctttggaa gccaacgcgg aagaacggct cacgcgcggg
     3841 ttcatctcaa tcacaatcat ctcgccgttt gcagggttca ccgcaaactg cacgttcgag
     3901 ccgcccgtgt ccacgccgat ttcgcgcaat accgccagcg aagcattacg cataatttga
     3961 tattccttgt ccgtcagcgt ttgcgccggc gcaaccgtaa tcgagtcgcc tgtatgcacg
     4021 cccatcgggt cgaagttttc aatcgagcag atgatgatgc agttgtcgtt cttatcgcgc
     4081 accacttcca tctcgtactc tttccagccg agaacggact gctcaatcaa cagctcgtgc
     4141 gtgggcgacg catcgaaacc gcgttcgcaa atcgccaaaa actcgtcttt attgtaggca
     4201 atgccgccgc ccgaaccgcc catggtgaaa gaaggacgaa tcagggtagg gaagccgacc
     4261 tgttcttgcg ccgccaaagc ttcgttcatc gtgtggcaga caaaagattt cgggcaggag
     4321 aggccgattt tctccatcgc ctccttaaag cggccacggt cttctgcttt gtcgatggcg
     4381 tcttcggtcg cgccgatcag ctcgacattg tatttcgcca gcacgccgtt gcgcgccaaa
     4441 tccagcgcac agttcagcgc agtctgacca cccatggtag gcagaatcgc gtcaggacgc
     4501 tctttggcaa taattttttc caccgtctgc cacataatcg gctcgatgta ggtaacatcc
     4561 gccatttcgg ggtcggtcat aatcgtggcg gggttggaat tcaccaaaat gactttatag
     4621 ccttcttcac gcaaggcttt gcaggcctgt gcgcccgaat agtcaaattc gcaggcctga
     4681 ccgataacga tagggccggc gccgatgata aggatggatt ttaggtcggt acgtttgggc
     4741 atggtgggtc tttcttttga agaatttgga ttattgacga ttggtaactt ctctaattat
     4801 ttctgcatct gttttttcat cgcttggatt atctgaaaag aaaaaactgt aattactatc
     4861 ggctttaaat ttttcgtata gctctgggcg caaaattttt gctttttcac tattttccac
     4921 gctcctgcga ttaggcttgc cagccttatc tttcccattt ctacggacat catagcaata
     4981 aatagtgtta ggagaattag gttctaaacg agtagctaca atctttttat aaattatgtc
     5041 taaatctttt ttagtttgtg ttggaatgaa atctgcaaaa agattactaa aactctgaaa
     5101 aatttctctg attttcgcag caccagcctg aacattaatt ttaaaaaaat taataaaatc
     5161 agtagcactt ttaaaatgaa cttcataaaa atcagcatac gcaaatattg aattttcact
     5221 attcaaatta aaaaactcta attttgcttg tgcataatca tctggtctag gttcatcagg
     5281 tttataccta gtcaaaataa cggcgtattg cctttgatta taattaaaag gaaatgcttc
     5341 aattattcta tttttgtttc tcatatcatt aagcaaatac tttaaattat tgaaattaat
     5401 aggtattgac atatttaaac tcctgcgaat atgttgcaat aaacgtagca agcgtaggtt
     5461 gggttgaaaa cccaacacaa accgcttaat tgtctaaaat gttgggtttg ctaaaaccta
     5521 acttgtatat cgaaaccccc gtatcgtagc aagccgtagc ccgtatgtag ggtatgtgcg
     5581 gtacgtatgc acgcgttctc cattttccct gcaaccccaa cccaccgcgt gcgtgccttg
     5641 cggcacactc tctgccgatg agttcaaagg tcgtctgaaa aaacgttcgc ggcgtttgtt
     5701 ccacctgcct tgcgataaag gctgcctgaa aaattcccgc tgttgatatt tcgggcattc
     5761 tttctcaaac cgcctgcaac cgcaaaatcc gctgcaacct gccccctccc ccgtggggga
     5821 gggctgggga gatggaattc tccgaatttc ggcaaccttc cccaatgcct tcaccgccta
     5881 aatacaagcc ttgcggcttg ttgccctctc tctaacgctt tcccacaggg agagaggacg
     5941 gggcggctgt tggaattaag ggttctgcag gaaaaacagg ttgtgcgggc tgttttttgt
     6001 ttttcagacg gtcttctaaa cgctcaattc aagcagcctg taccctacct gtatcctata
     6061 attttcaaaa ctctaatcaa aattaaccgt ttgttattgc gcatactgct tttccaattc
     6121 ctgcaatacg cgcaggattt ccgccagtac atcgtttgtc tgctgcaaaa tttcgtgatt
     6181 ccaaaaacgc agcacggtaa agcccaggct gttgagatat gccgtccgcg cgtggtcgta
     6241 tacggcttgt tccgcgtgct gcccgccgtc tgcttcgaca atcagcttgg gcgttacgca
     6301 cataaaatca acaatataat tccccatcgg ctgctggcgg cggaatttat agccgttcag
     6361 acggcctgcc cgcaggtgct gccacaattt tgcttccgcc tcgctcattt cttggcgcat
     6421 ggctttggcg cgttgatgca ggacggggtt gtcggcggtt aaaagtttct cgtgcgtgct
     6481 cattctgttg tcgggttact gtttgtgtaa gccttacagt ttgttgccct ctctctgact
     6541 ctctcccatg ggagagagga cgtggtggcg gttgggatta agggttttgc aaggaaaaca
     6601 ggttgtgcag gctgcttttt atttttcaga tgacctgaaa caacagctgc aaatctaagt
     6661 tgcctttcaa accgcctgca accgcaaaat ctgctgcaac cttctccctc ccccgtgggg
     6721 gagggccggg gagagggcat tccccgaatt tcggcaacct tccccaacac cctaaccgcc
     6781 caaacacaag ccttgcggct tgttgccctc tctctaacgc tttcccacag ggagagagga
     6841 cggggcggct gttggggtta aagaggctgc aaggaaaaca gcttgtgcag gctgtttttt
     6901 attttgcata agccgtctga aaatcaataa ctgctgattt ccagcaggtt gccatcggga
     6961 tcgcgcaggt aaaccgattg gattttgccc attgcgcctg tgcgcgctac gatgccgctt
     7021 aaaggtttga tgccgtgtgc ggataattcc tgtaaaaccg tttccagtgg cgtatcggtc
     7081 agcaggcata aatccgctgt gccgcaggcg gcgtgttgcg cgttaggctg aatttccgca
     7141 ccgcgcccgt gtaggttgat tttctgactg ccaaacaaca aagctttacg gtcgctgcca
     7201 aatgaaactt cttccatgcc caaaacttgt gtgtatagtg aattaacaaa aatcaggaca
     7261 aggcggcgag ccgcagacag tacaaatagt acggcaaggc gagccaacgc tgtaccggtt
     7321 taaatttaat tcactataaa acgcgatggt tcggtcaatg tcggcaacag ttagtactag
     7381 atggtcgagt gcgctaatct tcatttccag ctttgttttt cagacggcag cagcatactg
     7441 ttgccgtctg aaaaccatta tgcccgtttt gccgctttca tattgccaat gaatttgtca
     7501 aacaaatagc cgacatcttg cggaccgggg ctggcttcgg ggtggccttg gaagcagaac
     7561 acaggtttgt cggtcagctc gatgccttgc aaagtgttgt caaacaagga tttgtgggta
     7621 atgcgtgcgt tagcgggcag ggtgtcggca tcaacggcaa aaccgtggtt ttggctggtg
     7681 atgacgactt tgccgctgtc caaatcttgc acagggtggt tcgcaccgtg gtggctgaag
     7741 cgcattttca gggttttcgc gccgatggcg aggctgatga gctggtgtcc caagcaaatg
     7801 ccaaaaatcg gtttgccgct ttctatcagt ttttgtacgg ctttgatggc gtaggtgcaa
     7861 ggctcggggt cgccggggcc gttggatagg aatacgccgt cagggttgag tgccaacacg
     7921 tcttccgcgc tcgtttgggc ggggacgacg gtcaggcggc agccgcgcga ggcgagcata
     7981 cgcaggatgt tggttttcac gccgaaatcg taggcgacga cgtggtaagg ctgttcgtca
     8041 ggggtaacga aacccttgcc caatgcccat tcgccttccg tccattcgta agtttccgtg
     8101 caggaaactt cttttgccaa atcttttccg accatgctgc cgaacgcggc gatgagttct
     8161 tgcgcttttt cgattgtggc atccgcaccg gtcagaatcg caccgccttg cgcgcctttt
     8221 tcgcgcaaca gcgtggtcag gcggcgggtg tcgatgtcgg cgatggcgac ggttttgttg
     8281 cgtaccagat agtcgtgcag gctttcggag gcgcggaagt tgctgtgcaa gagcggcagg
     8341 tcgcggataa tcaggccggc ggcataaacg ctgcggcttt cttcatcttc ggcgttggtg
     8401 ccggtgttgc cgatgtgtgg gtaggtgagg gtaacgattt gtttgcagta ggacgggtcg
     8461 gtcaggattt cctgatagcc ggtcatcgaa gtattgaaca cgacttcgcc ggaagtcgaa
     8521 ccttcgtaac cgattgatgt gccgtgaaat acgctgccgt cagcgaggac gaggagggcg
     8581 ggggtgctca tgatgggaat cctgttttta ataaaattct tgacaatgcc gtctgaaggg
     8641 acttcggcag ggggcatccg gtcggaaaaa tccggtcaaa aaaaaacacg ccgcgcaaga
     8701 tagacgcgta acgtgctttt cgggcaagtg cctataccct gaaagcagca tattttaaga
     8761 cgaaacatac ccgatttcaa gcgcaaacgc ggcaggcggg ctgatatgtt cgcgcgaaaa
     8821 cggaaatccg gggcataaaa ccgcccgctc cgcgcccact atgcccgtta cgcatttccc
     8881 cacgtccgcc cggcgaaact atgggaatac c
//
LOCUS       DAWGRZ010000073         8876 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000073 DAWGRZ010000000
VERSION     DAWGRZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8876
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.073"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(134..469)
                     /locus_tag="WHT44_001188"
     CDS             complement(134..469)
                     /locus_tag="WHT44_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ2533374.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAATKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            598..1503
                     /gene="mtrA"
                     /locus_tag="WHT44_001189"
     CDS             598..1503
                     /gene="mtrA"
                     /locus_tag="WHT44_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ2533375.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1565..2053)
                     /locus_tag="WHT44_001190"
     CDS             complement(1565..2053)
                     /locus_tag="WHT44_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ2533376.1"
                     /translation="MKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2200..3102
                     /locus_tag="WHT44_001191"
     CDS             2200..3102
                     /locus_tag="WHT44_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ2533377.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3143..4273)
                     /locus_tag="WHT44_001192"
     CDS             complement(3143..4273)
                     /locus_tag="WHT44_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ2533378.1"
                     /translation="MTKHLPLAVLTALLLAACGGSDKPPAEKPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4478..7102
                     /gene="alaS"
                     /locus_tag="WHT44_001193"
     CDS             4478..7102
                     /gene="alaS"
                     /locus_tag="WHT44_001193"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ2533379.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERIAAVMQHVHSNYEIDLFQDLLKAVARETGAPFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALVKGGKTLGGEI
                     IFKLYDTYGFPYDLTADICRERNIEPDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVNELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGRHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAILANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGEEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKPLTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAD
                     KLPAVLDSVKDWVGAKLV"
     gene            7363..7794
                     /locus_tag="WHT44_001194"
     CDS             7363..7794
                     /locus_tag="WHT44_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533380.1"
                     /translation="MSISLIGLHITIAIILFFTTNFMGKKSSIFGYYQLSFSEENHSP
                     AFNIFYRAFTPILFIVIFSWVVTSLEIPISLEKINYVVIYYFIIRLLSVFVFEKTHIV
                     NWFNQLTIPILSITLSFIVYNKMILPKSFLLPSSQEVATTF"
     gene            8094..8381
                     /locus_tag="WHT44_001195"
     CDS             8094..8381
                     /locus_tag="WHT44_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533381.1"
                     /translation="MQVTSAEYLSNEESIKKGGNILMEKYNEKYNESIDGNKTLYKSY
                     YESRRESIKNYNPDAKYINEIESIYMMLGEIYPNAPDFMSPHFEGDCSEGE"
     gene            8580..8666
                     /locus_tag="WHT44_001196"
                     /pseudo
     CDS             8580..8666
                     /locus_tag="WHT44_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
BASE COUNT         2309 a         2258 c         2089 g         2220 t
ORIGIN      
        1 ttcacgctta ccccttgttt ctcaaatgcc gtctgaaata agcggcttaa tatattgttt
       61 acagtattgg gaagcataac agacaaaatg ccgtctgaaa tattttcaga cggcatttct
      121 tatccgaaac ggattatttt tgcgtttcaa ccgcttccaa tgcacgcagg gcataagtgt
      181 aagcggcacc cgcattcagg gcaatggcgg ttgccaatgc acctgcgatt tcgctgtcgg
      241 tcgcaccggc tttggtggcg gcggcggcgt gaacactgat gcagctctca caacgtgtag
      301 taatggcaac ggcgatggca atcagttcgc gtgttttagc atcaagtgcc tctgcagctg
      361 ccgcttgttc caatgcgccg taggcctgca gcattttagg atgcgcctta cccagctcgc
      421 cgaacgattt tttaaccaat gcggtatgtt ctttccaatc tttaaacatt ttcttttcct
      481 ttctcttgcg tttaaccctg atacgcgctt gcgtatctgt tttcgatgtg cgtattattg
      541 caattattca gttgtgtttc tcgtttaatc atctcatttt atggttcaaa aagatttatg
      601 gacattctgg acaaactggt cgatttcgcc caattgacgg gcagtgtgga tgtgcagtgc
      661 cttttgggcg gacaatggtc ggtacggcat gaaaccttgc aacgcgaagg attggtacac
      721 attgttacat cgggcagcgg ctatctctgc atcgacggcg aaacttcccc gcgtccggtc
      781 agtacagggg atattgtatt tttcccgcgc ggcttgggtc atgtgttgag ccacgacgga
      841 aaatgcggag aaagtttaca accggatatg cggcagcacg gtgcgtttac ggtcaagcag
      901 tgcggcaacg gacaggatat gagcctgttt tgcgcccgtt tccgctacga cacccacgcc
      961 gatttgatga acgggctgcc tgaaaccgtt tttctgaaca ttgcccatcc gagtttacag
     1021 tatgtggttt caatgctgca actggaaagc aaaaaacctt tgacggggac ggtttccatg
     1081 gtcaacgcat tgtcgtccgt cctgctggtg cttatcctgc gcgcctatct cgaacaggat
     1141 aaggatgtcg aactctcggg cgtattgaaa ggttggcagg acaaacgttt gggacattta
     1201 atccaaaagg tgatagacaa accggaagac gaatggaatg tcgacaaaat ggtggcggct
     1261 gccaatatgt cgcgcgcgca actgatgcgc cgtttcaaaa gccgggtcgg actcagcccg
     1321 cacgcctttg tgaaccatat ccgcctgcaa aaaggcgcgt tgctgctgaa aaaaaacccg
     1381 gattcggttt tgtcggtcgc actgtcggta ggctttcagt cggaaacgca cttcggcaag
     1441 gcgttcaaac ggcaatatca cgtttcgccg ggtcaatacc ggaaagaagg cgggcaaaaa
     1501 taaatcgggg cttcaaacgc aaatgccgtc tgaaaaggct ttcatacagc atttgcgtac
     1561 cgcgtcattt caagggctgc atcttcatca cttccatcaa aaagttggta aatgcggggt
     1621 tgttgggttt gacatccata tttttccaac gctgctgcca gccgcgcaag gcattctgga
     1681 tatacagctt ggactgttcc gtattgattg cgccccgctg gctgtctatc gccgaacgca
     1741 ggtagatttc atacatactg tcatcgacgg cattgcgtcc gaccaggcgt tttctgaagt
     1801 tgttcagata ttgcgccgcc tgaaccttgg tcattttacc gatacccacc tgatagccca
     1861 agcgcgtcgc ttcatcgctg attttggcaa catccgtcca atgcgaagag gcaaggcgga
     1921 aaccttttgc aggtgcttcc gttttgacgg tattgatagg attcacgggg atttccgtca
     1981 atgtgggcac ataaatagac tggcagccgg aaagaactgc cgcaatggaa agagggataa
     2041 ggtatttttt catgccccca ttataatcaa gtttgccttg agaaaacaaa ttgttcggca
     2101 agaaaaataa aatttcggca tcagaagcag gcaaaaacac attccacaag ccttgccgca
     2161 aggtttacaa tccgaccgtc cttatcgcaa cgaccgttta tggataccgc aaaaaaagac
     2221 attttaggat cgggctggat gctggtggcg gcggcctgct ttaccattat gaacgtattg
     2281 attaaagagg catcggcaaa atttgccctc ggcagcggcg aattggtctt ttggcgcatg
     2341 ctgttttcaa ccgttgcgct cggggctgcc gccgtattgc gtcgggacac cttccgcacg
     2401 ccccattgga aaaaccactt aaaccgcagt atggtcggga cgggggcgat gctgctgctg
     2461 ttttacgcgg taacgcatct gcctttggcc actggcgtta ccctgagtta cacctcgtcg
     2521 atttttttgg cggtattttc cttcctgatt ttgaaagaac ggatttccgt ttacacgcag
     2581 gcggtgctgc tccttggttt tgccggcgtg gtattgctgc ttaatccctc gttccgcagc
     2641 ggtcaggaaa cggcggcact cgccgggctg gcgggcggcg cgatgtccgg ctgggcgtat
     2701 ttgaaagtgc gcgaactgtc tttggcgggc gaacccggct ggcgcgtcgt gttttacctt
     2761 tccgtgacag gtgtggcgat gtcgtcggtt tgggcgacgc tgaccggctg gcacaccctg
     2821 tcctttccat cggcagttta tctgtcgtgc atcggcgtgt ccgcgctgat tgcccaactg
     2881 tcgatgacgc gcgcctacaa agtcggcgac aaattcacgg ttgcctcgct ttcctatatg
     2941 accgtcgttt tttccgctct gtctgccgca ttttttctgg gcgaagagct tttctggcag
     3001 gaaatactcg gtatgtgcat catcatcctc agcggtattt tgagcagcat ccgccccact
     3061 gccttcaaac agcggctgca atccctgttc cgccaaagat aaaaaatgcc gtccgaacat
     3121 ccttcagacg gcatatcggg ctttatttcc ccgccttcac atcctgccac tggcgcacca
     3181 taaacttcaa tgccgccggc tggataggca ccatgataaa gctgtttttc aaatcctcct
     3241 cggttgggaa aatcgtattg tcgtttttaa attcgtcttc catcagctca cgcgcaggct
     3301 tgctcgaagg cgcgtaagta acgaaattgc cgtttttcgc cgacacttcc gggtcgagga
     3361 agtcgttgat gtatttgtgc gcgttggcga cgtttttcgc atctttcgga atcacgaaag
     3421 aatccaccca aatccccacg ccctctttgg gcatcatcac gcggattttt tccttgccgc
     3481 ccgcttcttc ggcacggcgt ttggcgatgt tcaaatcgcc gccgaaaccg attgttacgc
     3541 aggtatcgcc gcgcgccaaa tcatcgataa agccggacga agtaaagcgt ttgatattgg
     3601 ggcggttttt cttgagtagg gcggttgcct ccctgatgtc ttccgtattg ctgctgttcg
     3661 ggtttttacc caaatagttc aacaccatag gatagatttc cgccgcgctg tccaaatagc
     3721 tgatgccgca ttgcttgagt ttggacgtgt attcggggtc gaacaccaaa tcccactggt
     3781 tgtccggcag cttgtccgta cccaaagcct ttttcacgcg ttcggtattg atggcgaagg
     3841 tatttgtccc ccaataaaac ggcacggcgt attcgtggcc gggatcgacc ccgtccatca
     3901 gcctcatcat ttcggggttg aggtgtttat aattgggaat cagcgactta tcgattttct
     3961 gatacgcacc tgccttaatc tgcctgccca caaacgcatt ggacggcgcg acaatgtcgt
     4021 aaccggactt gcctgtcagc accttgcttt ccagcgtttc atcgctgtcg tacacatcat
     4081 aagtaacctt gatgccgttt ttcttttcaa aatcggcaac ggtttccgga tcgacatatt
     4141 ccgaccagtt gtaaattttc aatacgtttt ggttttccgc cggtgccggt ttttcggcag
     4201 gcggtttgtc cgaaccgccg cacgctgcaa gcagcaaagc agtcaggacg gccaggggca
     4261 gatgtttggt cattatcatt ccttgcatat cgggttggag aaagcggcca ttatagccga
     4321 tattggcaac agggcttcag acggcattca aaatcccgcc acactcttcc gaaaaccgcc
     4381 gcttccatag ctagaaacag ggatttgcgg taagataccg ccgttcgttt tccctgcttt
     4441 taccatgaca agacatttga gagacattga aaaaattatg aaaacctccg aactgcgcca
     4501 aaaattccta aaattttttg aaaccaaagg ccacaccgtc gtccgctctt ccagcctcgt
     4561 gccgcacgac gacccgaccc tgctgtttac caacgcgggc atgaaccagt ttaaagacgt
     4621 attcttaggt ttcgacaaac gcccgtacag ccgcgccacc accgcgcaaa aatgcgtacg
     4681 cgcaggcggc aaacacaacg acttggaaaa cgtcggctac accgcccgcc accacacctt
     4741 ctttgaaatg atgggcaact tctccttcgg cgactacttc aaacgcgacg ccatccactt
     4801 cgcttgggaa tttctgactt cccccgaatg gctcaacatc cctaaagaca aactgttggc
     4861 gaccgtttac gcggaagacg acgaagccta caacatctgg ttgaacgaaa tcggtatgcc
     4921 gtccgagcgc atcgtccgca tcggcgacaa caaaggcgcg aaatacgcat ccgacaactt
     4981 ctggcaaatg ggcgacaccg gcccttgcgg cccctgctcc gaaattttct acgaccacgg
     5041 cgaagaaatc tggggcggca ttcccggcag tcccgaagaa gacggcgacc gctggatcga
     5101 aatttggaac tgcgtattta tgcagttcaa ccgcgacgaa caaggcaata tgaacccgct
     5161 tcccaaacct tccgtcgata ccggtatggg cttggaacgc atagccgccg tcatgcagca
     5221 tgttcacagc aactacgaaa tcgacttgtt ccaagacctg ctcaaagccg ttgcccgcga
     5281 aaccggcgcg ccgttcagta tggaagaacc cagcctgaaa gtcatcgccg accacatccg
     5341 ctcctgctcg ttcctgattg cagacggcgt cttgccttcc aacgaaggcc gcggctacgt
     5401 attgcgccgc attatccgcc gcgccgtgcg ccacggttac aaactgggtc aaagcaaacc
     5461 gttcttccac aaactcgttg ccgatttggt caaagagatg ggcggtgcct accctgaatt
     5521 gaaagaaaaa caagcccaaa tcgaagaagc attgaaaaac gaagaaagcc gttttgccca
     5581 aacgctggaa accggtatgg ctttgttgga aaacgcgctg gtcaaaggcg gcaaaacact
     5641 cggcggcgaa atcatcttca aactctacga tacctacggt ttcccatacg acttgactgc
     5701 cgacatctgc cgcgaacgca atatcgaacc ggacgaagca ggcttcgagc gcgaaatgga
     5761 agcccaacgc gcacgcgcac gcgccgccca aagcttcaaa gccaacgccc aactgcctta
     5821 tgacggtcaa gacaccgagt ttaaaggtta tagcgaacgc caaaccgaat ccaaagtcct
     5881 cgccctctac aaagacggcg agcaagtcaa cgaattgaac gaaggcgaca gcggcgcagt
     5941 cgtcatcgac tttaccccgt tctatgcaga atccggcggc caagtcggcg atgtcggcta
     6001 tatcttctca ggcgaaaacc gctttgaagt acgcgatacc caaaaaatca aagcggccgt
     6061 attcggtcaa ttcggcgtac aaacttcagg ccgtctgaaa gtcggcgaca gcgttaccgc
     6121 caaagtggac gacgaaatcc gcaatgccaa tatgcgcaac cacagcgcaa cccacttgat
     6181 gcacaaagcc ctgcgcgatg tattgggcag acacgtcgaa caaaaaggct ctttggttac
     6241 cgccgaatcc acccgtttcg acatttccca tccccaagcg gtaactgccg aagaaattgc
     6301 cgaagtagaa cgccgcgtca acgaagccat tttggcgaac gttgccgtca atgcagccat
     6361 tatgagcatg gaagacgcgc aaaaaaccgg cgcgatgatg ctcttcggcg aaaaatacgg
     6421 cgaagaagtg cgcgtactgc aaatgggcgg tttctctacc gaattgtgcg gcggcacaca
     6481 cgtttcacgc accggcgaca tcggcctctt caaaatcatc agcgaaggcg gtattgccgc
     6541 aggcgtgcgc cgtatcgaag ccatcaccgg cctgaacgca ctcaaatggg cgcaagagca
     6601 agagcgtttg gtgaaagaca ttattgccga aaccaaagcc caaaccgaaa aagacgtact
     6661 ggcaaaaatc caagcaggcg cggcacacgc caaagcattg gaaaaagaat tggcacgcgc
     6721 caaagccgaa ctcgccgtcc acgcaggcgc caaactcttg gacgatgcaa aagacttggg
     6781 cgcagccaaa ctcgttgccg cccaaatcga agccgacgca gccgccctgc gcgaaatcgt
     6841 taccgattta accggtaaat ccgacaacgc cgtgattctt ttagcggcag taaacgacgg
     6901 caaagtctcc ctgtgcgccg gcgtatccaa accgttgacc ggcaaagtga aagcaggcga
     6961 tctggttaaa tttgcagccg aacaagtcgg cggcaaaggc ggcggcagac cagatttggc
     7021 gcaagccggc ggcacggatg ccgacaaatt gcccgccgtg ttggatagcg tgaaagactg
     7081 ggtcggcgcg aagctggttt gatgtgggaa aggcagcctg aaaggtttca ggctgccttt
     7141 tgtgcaaaga ggccgtctga aaggtctcgt ttgccgtagg ttgggtcgcg acccaacaaa
     7201 ttttgtgaag tataaaaatg ttggtcatga cccaacctac ctgccttttt gtacaaagag
     7261 gctatctgaa aggccttgtt tgccgtatgg tgggtcgcga cccagcagat ttttattagg
     7321 gtatgaccca agctacttgc tacgataaaa aaggattttt aaatgagcat tagccttatt
     7381 ggactacaca ttaccatagc aatcattttg ttttttacta caaattttat gggaaaaaaa
     7441 tcatctatat ttggctatta ccaactgtct tttagcgaag aaaatcactc tccggcattt
     7501 aatatttttt acagagcatt tacccctata ttatttatcg ttattttttc ttgggttgtt
     7561 actagtcttg aaattcccat ttctcttgaa aagataaact atgtagtaat ttattatttt
     7621 ataattagat tgttatctgt atttgttttt gagaaaacac acatagttaa ctggtttaat
     7681 caactaacaa tacccatact atccataaca ttatcattta tagtatataa caaaatgatt
     7741 ttgcccaaaa gttttctact tccatcctca caagaagtag ctactacttt ttgaatagcg
     7801 cttggtggtt acatatataa tatattaaat aatgaatcag ggcatttaaa atcttataaa
     7861 gaaagaagag taaattatgt aaaacacatg cacaaaaaat ttgaaagtta ttttggtaaa
     7921 attatagata aaataatcaa agaggatagt tataataatg atgatttttt aaccgataag
     7981 aaaaaagcac taatatattc agttttaatt tatgagaatt ttaatagggg actagtttat
     8041 agatattttg aaaaaaatta ttttgtactg gtagaataaa aacatttgga ataatgcaag
     8101 taacctcagc agagtacctt tccaatgagg aaagtataaa aaaaggcgga aatattctta
     8161 tggaaaaata caatgaaaaa tataatgaat ctattgatgg caataaaact ctctataaat
     8221 catattatga atcaagaaga gagagtatta aaaactacaa cccagatgca aaatacatta
     8281 atgaaattga atcaatttac atgatgcttg gagaaatcta tccaaatgca ccagacttca
     8341 tgtcaccaca ttttgagggg gactgctctg agggggaata aaatcattat ttattcttta
     8401 ttagttatta gcaggatttg tcgggcataa atgcccgacc tacaaattca attttttcaa
     8461 acctctgcca aatattttca tctttgcaag gctgtctgaa aacccaaacc ccattttcag
     8521 acggcctttt ttcgctaaaa tccccatacc gttcaatccg aaaacacagg agaatcatca
     8581 tggaagttac catctccgcc atcatcaatg gcgaatttgc cgaccaatac ggcaagcgcg
     8641 gtagtcagtt taatgaaaac gggatgctga tttaattcta tttcctttga aactaccaat
     8701 aacctgcctc catcataaaa ctaaaagcaa gccgtagcct gcattcccac aaaccgcgtg
     8761 cgttgccatg tcacacaccc tacctgcggg cgacgcaaac cttaagagac ctttgcaaaa
     8821 ttccccaaaa tcccctaaat tcccaccaag acatttaggg gatttctcat gagcac
//
LOCUS       DAWGRZ010000074         8815 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000074 DAWGRZ010000000
VERSION     DAWGRZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.074"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            179..1354
                     /locus_tag="WHT44_001197"
     CDS             179..1354
                     /locus_tag="WHT44_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ2533382.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(1565..2113)
                     /locus_tag="WHT44_001198"
     CDS             complement(1565..2113)
                     /locus_tag="WHT44_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ2533383.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNTESK"
     gene            complement(2116..3612)
                     /gene="ccoG"
                     /locus_tag="WHT44_001199"
     CDS             complement(2116..3612)
                     /gene="ccoG"
                     /locus_tag="WHT44_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ2533384.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            4140..6119
                     /gene="tkt"
                     /locus_tag="WHT44_001200"
     CDS             4140..6119
                     /gene="tkt"
                     /locus_tag="WHT44_001200"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ2533385.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQAALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYVISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQAAVLPEGLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            6268..7656
                     /gene="fumC"
                     /locus_tag="WHT44_001201"
     CDS             6268..7656
                     /gene="fumC"
                     /locus_tag="WHT44_001201"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ2533386.1"
                     /translation="MSTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLSGKLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIHLLGDACNSFNEHCAIGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(7721..8644)
                     /locus_tag="WHT44_001202"
     CDS             complement(7721..8644)
                     /locus_tag="WHT44_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ2533387.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALIVVGGAVTAAVGDRLFKRR"
BASE COUNT         2226 a         2519 c         2158 g         1912 t
ORIGIN      
        1 cactataata ttttctctcc cgattgaaac aggcgtaaca gaatatgccc gaagctccgg
       61 ctgctttctt gtttaccgcc gcgatattta gagtataata ccaaatttga gcaatagttc
      121 taaaacagtt agaaccattt ttcatgagcc tgactgattc gtacactcgg agaaactgat
      181 gcagaatatt tttgaccctt tggttattcg tggaaaatcc cttaccccca tcgtgcaagg
      241 cggtatgggg gtcggtgttt ccgcatcggg tttatccagc gcggtggcgc gtgaaaacgg
      301 tatcggaacg attgccagtg tggatttgcg ccaccttcac gaagacctac tcgccgaatc
      361 acaaatcaat ccgagtgaag agaaatatac atctttgaac tgtaccgcat tagacaggga
      421 aatccaaaaa gccaaaagcg cttcagaggg aaaaggactg attgcggtca acgtgatgaa
      481 ggcggtcaaa gaccacgccg cgtatgtccg ccaggcttgc gaatcagggg cggatgcggt
      541 tgtaatgggt gccggcctgc ctttagacct gccggaaatg accgagggct atcataaaga
      601 tgtcgcgctg ctgccgattc tgtccgaatc gcgcggtatt aatatcgtct tgaaacgttg
      661 gatgaaaaaa ggcatattgc ccgatgcgat tgtagtcgaa catcctgccc acgcggccgg
      721 acatttgggt gcatcaaccg ttgaaggcgt aaacgatgcc aagttcgact tcaaacgcgt
      781 gattgaggaa acgtttgaag ttttcaaaag tttagggctg gaaagcgaaa aaatcccgct
      841 tattcttgcg ggaggcatgg caaattttga aaaagtcaaa accgccctaa agaactgggg
      901 agcatccgcc gttcaaatcg gtacggcttt tgccgttacc gaagaaggag atgcacacct
      961 taacttcaaa aaaacgctcg ccggtgcgga aactgaaaaa gtagtcgaat ttatgtctgt
     1021 tgccggtttg ccggcgcgcg gtgtccgcac caaattccta gacagctaca tcaagcgtga
     1081 aagcaaactt cagacaaacg ccaaagccga cccgcgccgc tgtacccaag gtttaaactg
     1141 cctaaccagt tgcggtctgc gcgacgggct ttccaaagca ggacagttct gtattgatat
     1201 ccagcttgcc gccgcattcc gtggagaagt agataaaggc ctgttcttca gaggtaaaga
     1261 cccgctgccc ttcggcaatg ccatccgcac cgtccgcgag acgatacaat atctgctgac
     1321 ggggagcgaa cctgttgcaa cgctcggacg ctgacatcag gtaggatttg atttgcaaac
     1381 aaaatgccgt ctgaagggct ttcagacggc gtttttcagg ctgcgttccg gaatagtgtt
     1441 aaaaaataaa cgggatgaga tacatttatt ttcgtccgac aaatcaaacc atcgcgccga
     1501 atgatcaaat aatgcctgca cggcattaca tctggcaaag caatgcaatg aaaacacggc
     1561 ttttttattt gctttcagta ttattgaaaa gcttgtccat cggggtcaaa tcgaccgcat
     1621 tgccttggct ggtaatccat ttgttctcga cgcgccacac gcctgccgcg tcctccacgc
     1681 gcacatacca gtggttggtc ggcgaaaggg ttttgaacac cgcctcatat tccgccctgc
     1741 cgttctgcgc gctgccgacg ggcttgaggg cgacggtttg atcgtccgcc ttgcgggtcg
     1801 ggtgcatcag cagcaggttc aaaggctgtt tgccgtcaaa ctcgccgccg acaaacactt
     1861 ttgccgcatt catatcgggg gaaatgagaa cctgcacccc gatatgccgt ctgacggctt
     1921 cttcatcccg atgaagctgg atgtcgatat gtttgccgtc tttataataa tcgtccgtaa
     1981 ccaaatctgt cgcgtgctgc tgcgcgacaa aaaacatagc gacgctggcg atgacgacaa
     2041 aaatcggccc cgccatcaag atccacggcc agacgtgttt gtaccaaggt ttgattggag
     2101 tgttttgaga cacggttatt ctccgataaa ggttgcatct tcttccaaga cgaccggctt
     2161 gccgtcgggc gcgccgcttt cgcggtattg gaaggtaaat tcgatagggt ggctgccttt
     2221 gtccgcgtat tccggaatgg tggacacttg gacgggaagg gttaccgttt cgcgcggggc
     2281 aaccttgata ccgccttcgg gcagcccggt cagggcgatt tcgtcaaagc ctttgacact
     2341 tgcggtaatc agctgttctt tttcactttt gttgatgata cgcaggctgt atgcgttttc
     2401 cagccagcct ttggcgtttt cgcgcaccag tacgccacgg tctttcaaaa tatcgacctc
     2461 gaccattttg cgcgtggaca aaccggccag gaaggcaatg ataactaacg ccaacaccgc
     2521 gccgtaacct gccacgcgcg gtctgagcag ccgtttttta atgtcttttt cagaatattc
     2581 gtgttccagc gcgctttcgg tcgtataacg gattaatccg cgcggatagc ccattttgtc
     2641 cataatctca tcgcacgcgt cgatacaggc ggcgcagccg atacattggt attgcagacc
     2701 gttgcggatg tcgatgccga cggggcagac ttggacgcac atcgcacagt tgatgcagtc
     2761 gcccaaaccc gcctcttcct tattgaccgt tttcttgcgc gcgccgcgcg gttcgccgcg
     2821 ttccgcgtca taagaaacaa tcagcgtgtc cttgtcgaac atcgcgcttt ggaaacgtgc
     2881 atacggacac atatgcaggc atactttttc acgcataatg tgggcgaaga agaaggtcat
     2941 aaagccataa aacgctgcgg caaacatcgc gccgccacct gctgctccag tgaataaatc
     3001 gggaacgaac tggcggatag ggacaaacca gcctgcaaac gtgatgcccg tccacgcgca
     3061 gacaaggaaa atcagcaggt atttggtggc tttgatgcgg attttagtga aattccacgg
     3121 cgatttttcc agtttcagcc gtttgtttct atcgccttcg accaggttgt caatccacag
     3181 cataatttcg gtgtaaaccg tttgcgggca ggaatagccg caccacagtc gccctgcaat
     3241 cgtcgtccac caaaacagcc cgaaggcgca aatcatcagc agcaaggcaa ggtaaatcaa
     3301 atcgcccacc cccaacgaca atccgaaaat gaagaaatgc cgttcgggga tattgaaaac
     3361 gacggcctgc ctgccgctcc agttgaacca cggaatgacg taaaacacaa actgcgtcgc
     3421 caatacggcg gcgatacgca gtttggcgaa ccgtccttcc gcctttttgg gatggatgcg
     3481 ttcgccttcg ggatggattt gaatcacgct ggctcgcgga tcgaatgttt tttttgcttt
     3541 cggcgcggct tttgtttgtt cggacgtgcc gattccggat gccggactgc cggcttggtt
     3601 ttccgtggtc attctgcatt ccttagattt ttgattgatg gtttgcccgt taccgccgcc
     3661 gtttgctttt cagacgtcat ttttcttgtt ttttaaggcg ttgtgtttca agttttgaga
     3721 aaatccgttt ttcccaaaat atatttccgc tattgtacaa ctttatgcgc cgtccggatg
     3781 tatggggcgg atacatttcc catccgcatc aaaacgcctg gattttacct taccgcccga
     3841 acaaaatccg aatacggtta aaaaaaaaga ctaaaaaaac cgacaccccc atatcggcag
     3901 aaccgacggc gcaagctcat aaacaaacgc tatcgacaat ccggcacaca atctataact
     3961 ttttatttca aaaggaataa tggcaggctt cgcccgcaaa tcgaaaatcc ttccccgcct
     4021 gtcccctgcc gccgccttcc cacgcgtccg cccttttctt gaaagcataa gcgaatcggg
     4081 cgataatcaa cgctttccga ttatccactt atctgaaaca ccagcaagga aaatacaaaa
     4141 tgtctcaact ggcaaacgca atccgcttcc tctcggccga tgccgttcaa aaagccaatt
     4201 ccggccaccc cggcgcgcct atgggtatgg cggaaatggc ggaaacattg tggacgaaat
     4261 tcctcaatca caaccccgcc aaccccaaat tctacaaccg cgaccgcttc gtcctctcca
     4321 acggccacgc gtctatgctg ttgtacagcc tgctgcacct gaccggctac aacctaagca
     4381 ttgaagactt gaaaaacttc cgccaactgc acagcaaaac ccccggccat cccgaatacg
     4441 gctacaccga cggcgtggaa accacgaccg gcccgttggg gcaagggatt gccaacgcgg
     4501 tgggtatggc attggcagaa aaaatccttg ccgccgaatt taataaagac ggtttgaaca
     4561 tcgtcgatca ttacacctac gtctttatgg gcgacggctg tctgatggaa ggcgtatcgc
     4621 acgaagcctg ttcgctcgcc ggcaccttgg gcttgggcaa actgattgtt ttatatgatg
     4681 acaacaatat ttccattgat ggtaaagtgg acggctggtt taccgaaaac atcccgcaac
     4741 gctttgaaag ctacggctgg cacgtcgttc ccaatgtaaa cggtcatgac accgccgcca
     4801 ttcaagccgc catcgaagcc gcacgtgccg aaaccggcaa accgtccatc atctgctgca
     4861 aaaccttaat cggcaaaggc agtgccaaca aagaaggcag ccacaaaacc cacggcgcac
     4921 ctttgggcgc ggacgaaatc gaagccacgc gcaaacattt gggctggact taccccgcct
     4981 ttgaaatccc gcaagaaatt tacgatgcgt ggaatgccaa agaacaaggc gcgaaactgg
     5041 aagccgactg gaacgaactg ttcgcgcaat atcaagccaa atatcctgcc gaagccgcag
     5101 aatttgtgcg ccgtatggat aaaaagctgc cggacaattt cgatgaatac gttcaagccg
     5161 cattgaaaga agtgtgcgcc aaagccgaaa ccatcgccac ccgcaaagcc agccaaaaca
     5221 gcatcgaaat cttggcaaaa gagttgcctg aattggtagg cggttctgcc gacctgaccc
     5281 cgtccaatct gaccgactgg tcaaacagcg tctccgttac ccgcgacaaa ggcggcaact
     5341 acatccacta cggcgtgcgc gagttcggca tgggtgcgat tatgaacggt ttggtattgc
     5401 acggcggcgt aaaacccttc ggcgcgactt tcctgatgtt cagcgaatac gagcgcaatg
     5461 ccctgcgtat ggctgcgttg atgaaaatca accctgtatt tgtgtttacc cacgattcca
     5521 tcggtttggg cgaagacggc ccgacccatc aaccgattga gcaaaccgcc accctgcgcc
     5581 tgattccgaa tatggacgta tggcggccgt gcgacaccgc cgaatccttg gtggcttggg
     5641 cagaagccgt caaagccgcc gatcacccgt cctgcctgat tttcagccgt caaaacctga
     5701 aattccaagc gcgcagcgag caacaactga acgacatcaa acgcggcggc tacgtcatca
     5761 gcgaagccca aggcaacgcc caagccgtca tcattgccac cggctcagaa gtcgagctgg
     5821 ctttggaagc gcaaaaagcc ctcgccgcgc aaaacatcgc cgtgcgcgtc gtttccatgc
     5881 cgtccaccaa cgtattcgac cgccaagacg ccgcctatca agccgccgtc ctgcccgaag
     5941 gcctgccgcg catcgccgta gaagccggac acgccgacgg ctggtacaaa tatgtcggac
     6001 tgaacggcgc agtcgtcggc atcaaccgct tcggcgaatc cgcccctgcc gatttactct
     6061 tcaaagcatt cggctttacc gtggacaatg tggttgatac ggtgaaatcc gtgctgtaac
     6121 cccacaccta aacaaatgcc gtctgaaacc aattagggct tcagacggca tttttatatt
     6181 ctcgcggcca tgatgctttc tcatcccacc aatctccatt ataatatttg cgaatcactc
     6241 ttattcacat ttcaaaagga gaaacgcatg agcacccgta ccgaacacga cacgatgggc
     6301 aatgtcgaag tcccatccga agcctattgg ggcgcgcaga cccagcgcag ccgcaacaat
     6361 ttcaaaatcg gtggcgaaac cctgccgcag ccgttgattt atgctttggc attggtgaaa
     6421 aaagccgccg ctgccaccaa tgtttccctc ggtaggatta agcctgaaca ggcggatttg
     6481 attacgcagg cggcggatga tgtgttgagc ggcaagctcg acgggcagtt cccattggta
     6541 gtgtggcaga ccggttccgg cacgcagtcc aatatgaaca tgaacgaagt gctggcaaac
     6601 cgcgccaacg aaatcgccgg tacgggtttg gcggcttatc agcccgtcca tcccaacgac
     6661 catgtgaacc acgcgcaatc gaccaacgac gcattcccga ccgctatcca cgttgccgcc
     6721 gcgattgaaa tcaaccgcca cctcatcccc gccgtaaaag ccctgcgcga cacgttggac
     6781 aaaaaagccc aagctttcgc ccctatcgtc aaaatcggcc gcacccactt gcaagacgcg
     6841 acgccgctga ctttgggaca ggaattttcc ggctacgttt cccagcttga tcacggttta
     6901 ggccgtctga acgatgcgct taaagacttg tatgaacttg ctttgggcgg tacggcggtc
     6961 ggcacgggtt tgaacagcca tcccgaatac gccgaaaaag ccgccgccaa actcgccgaa
     7021 ttgtccggct tgccgtttgt cagcgcgccg aacaaatttg aagccctggg cggacgcgat
     7081 gccgccgttg ccgcttcggg cgcattgaaa acgctggcgg caagcctgaa caaaattgcc
     7141 aacgacatcc gttggctggc aagcggcccg cgttgcggtt tgggcgaaat caaaatcccc
     7201 gaaaacgagc cgggttcgtc cattatgccg ggcaaagtca acccgaccca atgcgaagca
     7261 atgacgatgg tgtgctgcca agtgttcggc aacgacgtta ccatcggtat ggcgggcgcg
     7321 tcgggcaatt tcgagctgaa cgtctatatg cccgttatcg cctacaacct cttgcaatcc
     7381 atccacctgt tgggcgacgc gtgcaacagc ttcaacgaac actgcgccat cggcatcgaa
     7441 cccgtgccgg aaaaaatcga ctatttcctg caccattccc tgatgctggt taccgcatta
     7501 aaccgtaaaa tcggttacga aaacgccgcc aaagtcgcca aaaccgccta caaaaacaac
     7561 aaatcgttgc gcgaaaccgc cgttgagttg ggcttgctga cgggcgaaga atttgacgaa
     7621 ctggtcgttc ctgccgatat ggttcatccg cgctaatcct tccctcaaat aaaatgccgt
     7681 ctgaaacctc gttcggacgg cattttccgt tgcctgcaaa ctagcggcgt ttgaacagcc
     7741 tgtcccccac cgccgccgta accgcacccc cgaccacgat cagtgcgcct gcataaccca
     7801 aaccgttcat atccggcgcg gcaaaagtat caggcatcac ataatgcccg agcaaagaaa
     7861 atattacggt aaacacgggg agcaaggttg ttaccgcgct gactttggaa gcctcccaat
     7921 gtttcaacgc ctcgccgaac gagccgtaac cgattaacgt attcaagcag caatacgcaa
     7981 aacaaaccca cgccaacgta ccgtccaaac ttccgatgtg tgccggttcg gcaaacggca
     8041 ggaacacggc ggcacttgcc gcataaatca acagcagaat ctgttgcggc ccgaattgcg
     8101 ccgacagcag cttttgcgcc acggcataac acacccatgc catactgcct gccgcacaca
     8161 gcaacacgcc cttcgcatac gcgcccaaac ccgacaactc gccgaattta tcgttaaaaa
     8221 acataagcaa accggcaagc agcaaaacca agccgatttt ctgagcggca gtcatccggt
     8281 ctttaaacac caacacaccg acaacaatca tcgtaaacgg cgaaatctgc cacaaaacct
     8341 gcgtcgtggt cggcgaaata taatgcagcc cttgggcaat cagcacaaag tttgccgaaa
     8401 tgcccgccac gccgagcagc agcagcctga atgagcacca agaaaaatcc cgccgcttcg
     8461 gcagccgccc gcccagtgcc agcaaaacaa acaataccgc cgccgccacg gtaaaacgca
     8521 cccacaccag cgtcggcgca tcgacaaact tcaatacctg ccgcacggca atcggcagcg
     8581 ttccccacgt catcgccgcc aaaagtgcca acgcgaagcc taggagcggc ctttggtttt
     8641 ccatcctgat tttcctattt ttaaacaacc gtattgccgg acgatgccgg tttgccgcat
     8701 cgggcaatga tggttcaagc gtttggcgtt tgattccaac cctttgattt caaacaaacc
     8761 ggctgaagct cggctattgc ttcgcgctat ttgaaaacac cgcctgaatt ttaaa
//
LOCUS       DAWGRZ010000075         8769 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000075 DAWGRZ010000000
VERSION     DAWGRZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8769
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.075"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(41..1132)
                     /locus_tag="WHT44_001203"
     CDS             complement(41..1132)
                     /locus_tag="WHT44_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ2533388.1"
                     /translation="MNAQTLIANVAEFVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGDGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNNYLNDGYDSLKTRLD
                     GAWAETERLAGLAWSGTPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            complement(1267..1815)
                     /locus_tag="WHT44_001204"
     CDS             complement(1267..1815)
                     /locus_tag="WHT44_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ2533389.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            complement(1970..2341)
                     /locus_tag="WHT44_001205"
     CDS             complement(1970..2341)
                     /locus_tag="WHT44_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ2533390.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            complement(2397..2504)
                     /locus_tag="WHT44_001206"
                     /pseudo
     CDS             complement(2397..2504)
                     /locus_tag="WHT44_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2636..4327)
                     /gene="dld"
                     /locus_tag="WHT44_001207"
     CDS             complement(2636..4327)
                     /gene="dld"
                     /locus_tag="WHT44_001207"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ2533391.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGNTPEEILTNLQGHHYQNKDITQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKVSFLPKHFSDKAMQFVSKFLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHGGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            complement(4494..4590)
                     /gene="ffs"
                     /locus_tag="WHT44_001208"
     ncRNA           complement(4494..4590)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WHT44_001208"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4851..8684
                     /locus_tag="WHT44_001209"
     CDS             4851..8684
                     /locus_tag="WHT44_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ2533392.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAIPLDANSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWNMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHESVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKTRWEWLLANLDTPLADYKARYGAA
                     VHAAPEAKNNESCFIAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLNGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSNLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLIEAFLYEEQT
                     RRGVSIKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQIGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKNVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPADYIVIEMAKYILGENWLDE
                     FVKKANNGGVEKVLL"
BASE COUNT         1931 a         2391 c         2430 g         2017 t
ORIGIN      
        1 gcgggaatga cgcggtgggg gtttcggttt ttccgcctgt ttattttgcg gcttcgattg
       61 ccgctatttc tttgcgtagg tgtttgatag cgggggttac gatggcaaca aacattgctt
      121 cgcggacgcg cctttgggcg ggactgcgca tcaggtagcc ttttgcgccg gaatgcaggg
      181 cggcggattg ggcggcggca agtgccagta cggcggcggc ttcgcgcagc ttgagggtag
      241 caaggttgtc gggcgtgccg ctccaagcca agccggcgag ccgttcggtt tctgcccacg
      301 cgccgtccag ccttgttttg aggctgtcgt agccgtcgtt gaggtagttg ttgacttcgg
      361 cgttgacgac gttggcgagg cggatgatgc cgaggctgcc gtcgattacg cccgcgccga
      421 tgccgatttg caggaggata aagcctgctt tgatgctttg gatgtagtcg gcaaactgtt
      481 cgggcgcggc gatgatgtct tcgtcgggga taaatacgtc tttgaaattc aggctgaagg
      541 tgcgcgtacc ttcgagggcg caaaattcgg ggcagttttg caggcttacg ccttcccatt
      601 gtccgcctgt gatgaacata acgtagccgt cgccgatttg ggcggtattc gcccagatgt
      661 ggtcttcacc gatgttggac acccacggca gcgcgccgtt gactgtgtag ccgccttcca
      721 cgcgttcggc ttggaggttg tgtttttcga tgtcggcaag gtgtttgacg gtattggaca
      781 tgcccgtacc cgccaatact ttgccttgca ggatgtcggc aaggtatttg tctttgacgg
      841 cccggttggg cgtttggtgc agataccacg cgcaagccgc ctgacaccac gcactgaaag
      901 aggttgcgcc gcattctttg ccgatttcgc gcaatacggc gatttgcgtt gccaaaccca
      961 agccgttgcc gccttcggct tctgtaccga ctgcgccgaa tccaccgatt gcgccgagtt
     1021 cgcgcataaa tgcttcgggg tagtatcctt tgcggtcgat gtcgtccact atgggtttga
     1081 gcttggtttt gacgaattcg gcaacgttgg caatcagggt ttgggcgttc atctttgttc
     1141 cttaaggttt gcggggaaat cgggggcgcg cctgatgcgc ggctgcttcc ctgccgctta
     1201 ggcggcttgt ttttgtttga gggggtgttt ttgaaaaacc gcccgatatt cgggcggttt
     1261 gccgtatcag gcgtaagcct gcaattcggg gttgatttcg gtttgtccga ggttgttgac
     1321 gtagttgcac agggttgcca aggctacgcc catcacgact tcgactgcct gctgctggtt
     1381 gtagcccgca tcgaaaaatg ctttgagttc ctcgtcggat accgcgcctt ttttcgccat
     1441 tacggcttgg gtgaaggcgg cgagcgcgcc gagtttggca tcgtcaaatt cgcctgctgc
     1501 caaagcgcgc gcggctttga cggattgttc ggacaggagt tttttcaggg ttgcgagttt
     1561 ggtgtgccct gccacgcaaa aaccgcattg gttggtacgg gcggcgatga tctggatgac
     1621 ttcgacttcg ccggcggtca ggctgttggc ggcgttgagc ttgccgactt cttggtaaaa
     1681 cgccaaggct tcgggggcgt ttgataatac gccgataagg ttggggataa agccgttgtt
     1741 ttgaagtacc gcctcgacgc gcgctttggc ggcttcgggg gcggtttcga gggtgtgtac
     1801 ggttaaacgt gccattttct ttccttgttc gcaaatattg ggtacgggcg catggtatgc
     1861 atttcggaac ggaataggaa agactgattg gttatgtgct gcaaacaaaa ggttataaga
     1921 aatgccgtct gaacattttt cagacggcat gatggaaaag aaaacgcgct tatcggcccc
     1981 cgcgcccgaa atattgcgcc aatgcggctt ggggattggc tgcctcaacc gtttcgggca
     2041 attcggtata gccgcgcgat aaaaggtggc gtgcataaag gctgttgccc tttgcgccga
     2101 accagacgga aaccgccaag cccaatacgt taaacgccga gtctgtggcg ggcgtgtcgc
     2161 cgtaaaccag ttgggcaaac acagccagca cgaatatcgc gcccgtcagc cccagcccca
     2221 aaccccacat tcttttgaaa cacgcccaca gcgtgccgaa caagagcccc ggccatgacc
     2281 agccttgttt gacggcttgg ggcggcaggg cgggatgggt gtagattttg tatggtttca
     2341 tcgtgtttcc ttttcggttg aaaccctgcc ctttgggaag gtaggatcag actttatagt
     2401 ggattaaatt taaaccagta cggcgttacc tcgccttgcc gtactatctg tactgtctgc
     2461 ggcttcgttg ccttgtcctg atttaaattt aatccactat atttgggagg cgcgcgcgcc
     2521 tgtgccggca tacggcttga aagcgattac ccgatgggga acttcaaacc cgacaatgcc
     2581 gtctgaacgg tgtcttgcct tcagacggca ttgcctgcct tcaaagcgga cgcgcttatt
     2641 ccgcccagtt tttctttttg ctggttttgc ctacgcccgg gttgaagctg ttggtcgggt
     2701 caagtttgcg gtaaaactgt ttgagcgcgg gcttggcttc gtacaaatgg ccgacgttgt
     2761 gttcggctgg atattgcgcg ccgcgttgat ccaagagatg cagcatttcg tgttccaatg
     2821 ccatgcagtc gttgcctttt ttgatgatgt aatcctgatg gaaaacgtgg cacatgaaat
     2881 gtccgtagta gagcttgtgg atgattttat tgtcgatttc cggcggcagt ttttcaaacc
     2941 agtcgcggtc gtcgcggcgc agggcgatgt caagcgcgac caagtcctcc acttcgtcgt
     3001 cgtgtacggc acggtagcgg atggcggctg aggcgacggc gaaacggtgc agcatcgcgg
     3061 cttgggtttc ttcggcgttg cactcgaaaa acgcgccgcc gtgatgtgca aaatactctt
     3121 tcaggaacgc gcgcgcctca tccacgcctt ttccgcccat tttcagaatc aggtggtgtt
     3181 cgtatttgtc gcggtaatcg cgcatggatt tgggcagatg gtcaggcagg aatttgctga
     3241 cgaactgcat tgccttgtcg gaaaaatgtt tgggcaggaa gctgactttt ttgccgaacc
     3301 tgtccacgcg tgccttcaaa tcaaataatt tcggcagttg gtgcgtaccg aattttttga
     3361 tgacgtaaaa cgtatctttg ccgtacacgt cggcaatgtc gaaagcgtgg cggtggatgt
     3421 attcgccgga aacgggcagg ctttcaaatt cgcccaaggc ggcgcggcgg atgtcggtca
     3481 gctcgttgat gtcgttcgtg ccgatgtaga acacggccgt ttgtttttct tgcggaaagg
     3541 tatccaagcg gacggcgaat accatcagct tgcccgcgca gcccgaggct tcgtaatggc
     3601 gcgctgggtc ggcattgaaa cgcgcggcgg tcggttcgtc cacttggcgg acatgttcgc
     3661 aataggcgtg gtcgtgtcct ttgcccgcgt cttgcgtgat gtctttgttt tgataatgat
     3721 gaccttgaag attggtcagg atttcttcgg gcgtgttgcc caagtctatg cccaagtggt
     3781 tgaccagttc caacctgcct tcttcgttga tttgggcgaa caacgccatt tcggtgtagg
     3841 ccgggccgcg ctgtaccaac gcgccgccag agttgttgca cacgccgccc aagacggacg
     3901 cgccgataca ggatgagccg ataaccgaat gcggttcgcg ccccaaaggt ttcagcagca
     3961 attcgagctg gttcagggtc gagccgggca ggcagacgac ttgttcgttg ttgttgatgg
     4021 tttggatgat gttcatccgc atggtgttca caatcacgat gtcgcggtcg taatcgttgc
     4081 cgtcgggggt cgagccgccc gtcaaaccgg tattcgccgc ctgcgtaatc acaatcacgt
     4141 ctgcttcgac gcacgcctgc agaattttcc acatttccag aatgcttccg gggcgaacca
     4201 ccgccaacgc cttaccctcg ccgaagcggt aaccttggcg gtattgttcg gttttcgcgg
     4261 ggtcggtgat gatgtatttt tcgcctacgg tttgggtcag tcttgacagt aattgtgatg
     4321 cgctcatggc agtttcctta aaattgtcgg caggtgcatt gcacattgga attgttttca
     4381 cattgtagtt atacgttatg gcaaagtaaa gaaaatgccg tctgaacggc tttcagacgg
     4441 catcggtgcg atacgggaac gccggaacat cgaagctccg gcgtttcaaa tagggcggcg
     4501 ggccaaaccc ccggcactgg cgcattggag tgggctgctg gcttccgccc ctgacccggt
     4561 gttccgattt gccatgcggg gagacccgcc tcagagaaac ggcattataa cgggttttct
     4621 gaaaaactca accgttttga tacggtcata cgccggaaac accacctaaa atttatattt
     4681 gataatattg tcaacaattt ctcaaagcgt tattttgttt ctataagggt atttcctgtt
     4741 tcggcattga aaagtatcaa aaattgaact acattatcgc cttttcaaac tcgcctgaaa
     4801 ccgacttttc agacggcatt caaataaaaa ctgccaaaca cggacacacc atgaccacga
     4861 ctaccgcccc tcagcgtatt cgggaaatcc cctacaacta tacttcctac accgaccgcg
     4921 aaatcgtcat ccgattattg ggcgacgaag cgtggcaaat cctgcaagac ctgcgcggtc
     4981 aacgcaaaac cgggcgttcg gcgcggatgc tgtttgaagt gttgggtgat atttgggtgg
     5041 tcgtgcgcaa tccgtatctg gtcgatgact tgctggagca cccaaaacgc cgcgccgcgc
     5101 tggtacgtga aatgcgccac cgcttaaatg aaatccgcaa acgccgcgac gataatcggc
     5161 aagtggatgt gttggttgcc gcagcagaaa aagcagtcga gcgttttgat agcagttttg
     5221 atgaaaccag ccaaaaacgg cggcagattt tggagcgttt gagcaaaatc accaagccgc
     5281 acaatattat gttcgacggg ctggcgcggg taacgcacgt taccgatgca accgactggc
     5341 gcgtggagta tccgtttgtc gtcgtcaatc ccgacacgga ggctgaaatc gcgcctttgg
     5401 tgcgcgcctt aatcgagctg gatttggtca ttattccgcg cggcggcggc acgggttata
     5461 ccggcggcgc gattcctttg gacgcaaaca gcgcagtcat caataccgaa aaactcgaca
     5521 agcatcgtgg tgttgaatac gttgagctgg caggcttgga cggcaagcat ccgattatcc
     5581 ggtgcggcgc gggcgtggtt acgcggcggg tggaagaaac cgcgcatcag gcaggtttgg
     5641 tgttcgccgt cgatccgact tctgccgacg cgtcatgcgt gggcggtaat gtggcgatga
     5701 acgcgggcgg caaaaaagcc gtgctgtggg ggacggcgtt ggacaacctc gcctactgga
     5761 acatggttaa ccctcaaggc gaatggctgc gtatcgagcg cgtgcgccac aatttcggca
     5821 aaatccacga cgaagaaacc gccgtgttcg acgttcacac gctggattca gacggcatca
     5881 atatcgttaa aaccgaacgc ttggaaatcc ccggccacaa attccgcaaa gtcggtttgg
     5941 gcaaagacgt taccgacaaa ttcttgagcg gcctgcccgg cgtgcaaaaa gaaggtacag
     6001 acggcatcat caccagcgtt gccttcgtgt tgcataaaat gccgaaatac acgcgcaccg
     6061 tgtgtatgga gtttttcggt acggtcgcca ccgccacgcc atctattgtc gaaatccgcg
     6121 actttttgct tgcccatgaa agcgtgcggc tggcgggttt ggaacatttg gactggcgtt
     6181 atgtccgcgc cgtcggctac gccaccaaag cggcgggcaa gggacgaccg aaaatggttt
     6241 tgctggcaga cgtggtttca gacgacgaag ccgccgtaga ggcagccgcc gaacacatct
     6301 gtgaactcgc acgcgcccgc gacggcgaag gctttatcgc cgtatcgccc gaagcccgca
     6361 aaaccttctg gctcgaccgc agccgcaccg ccgccatcgc caaacatacc aacgccttta
     6421 aaatcaacga agacgtggtc atcccgctcg aaaggctcgg cgagtattcg gacggcatcg
     6481 aacgcatcaa cattgagctt tccatccaaa acaagctcaa actctgtgcc gccttggagc
     6541 aatatctttc gggcaaactc cccatcgaca aaatgggcac tgacctgccg accgccgaac
     6601 tgttgggcga acgcggcaaa cacgccctgg cccacgtttc cgccgtcaaa acgcgttggg
     6661 aatggctgct cgccaatctt gacacgccgc ttgccgacta caaagcccgc tacggcgcag
     6721 ccgtccacgc cgcacccgaa gccaaaaaca atgaaagctg ctttattgcc ttccgcgatt
     6781 tccgcctgcg cgtgtctgtc aaagcggacg taatgaaacc gctttctgaa atcttcagcg
     6841 gcaaaaccga caccaaaatt atccaaggct tgggaaaaat ccacgcaaaa accgtacgca
     6901 gccgcgtctt tgtcgccctg catatgcacg ccggcgacgg taacgttcac accaatattc
     6961 cggttaactc agacgatgcc gaaatgcttc agacggcata ccgctcagtc gaacgcatta
     7021 tgaaaatcgc ccgttcgctt aacggcgtga tttccggcga acacggcatc ggcattacca
     7081 agctcgaatt tttaagcgac gaagaaatgc agccgttttg ggactacaaa aaccaagtcg
     7141 atccgaaaca caccttcaac cgtcacaaac tgatgaaagg ctcggactta cgcaacgcct
     7201 acacgccgtc cttcgagctg ttgggcgcgg aatcgctgat tatggaaaaa tcaaacctcg
     7261 gcacgattgc cgattccgtc aaagactgcc tgcgctgcgg caaatgcaaa cccgtctgct
     7321 ctactcacgt tccgcgtgcc aacctgctgt acagcccccg caacaaaatc ctcggcgtgg
     7381 gcttattgat cgaagccttc ttatacgaag aacaaacccg ccgcggcgtt tccatcaaac
     7441 actttgaaga actcatggac atcggcgacc actgcaccgt gtgccaccgc tgcgtcaaac
     7501 cctgccccgt caacatcgac ttcggcgacg ttaccgtagc cgtccgcaac tatcttgccg
     7561 attccggcca caaacgattt gcgcctgccg cagctatggg tatggcgttt ttgaacgcca
     7621 ccggcccgaa aaccatcaaa gcccttcgcg ccgccatgat acagatcggc ttcccagcgc
     7681 agaatttcgc ctacaaaatc ggcaaacttc ttccaatcgg cacgaaaaag caaaaagccg
     7741 aacccaaggc aaccgtcggc aaagccccga ttaaagaaca ggttatccat ttcatcaacc
     7801 gcccactgcc caaaaacgta cccgccaaaa caccgcgctc cttattgggc atcgaagacg
     7861 gcaaaagcat ccccatcatc cgcaaccccg ccgcgcccga agatgccgaa gccgtgttct
     7921 acttcccggg ttgcggctct gagcgtctgt tcagccaaat cggacttgcc gttcaagcca
     7981 tgctctggca cgtcggcgta caaaccgtcc tgccgcccgg ctatatgtgt tgcggctatc
     8041 cgcaagacgc aggcggcaat aaggcaaaag ccgaagaaat gagcaccaac aaccgcgtgg
     8101 ctttccaccg tatggcgaac accctcaact acctcgacat caaaaccgtc gtcgtcagtt
     8161 gcggcacttg ttacgaccag ctcgaaaaat accgctttga agaaatcttc cccggctgcc
     8221 gaatcatcga catccacgaa tacctgctcg aaaaaggcgt gaaactcgac ggcgtaaaag
     8281 gtcagcaata cctctaccac gacccctgcc atacccccat caaaaccatg aacgccaccc
     8341 aaatggccag cagcctgatg gggcagaaag tcgttttaag cgaccgctgc tgcggcgaat
     8401 ccggtatgtt tgccgtcaaa cggccagaca tcgccactca ggtcaagttc cgcaaacaag
     8461 aggaaatcga gaaaaacctc aaagagctgc cgcagggcga acccgtcaaa atgctgacct
     8521 cctgccccgc ctgcctgcaa ggcttgagcc gctacgccga cgacaacaat atgcctgccg
     8581 actacatcgt catcgaaatg gcgaaataca tcctcggcga aaactggctg gatgagtttg
     8641 taaaaaaagc caacaacggc ggtgtagaga aagtgttgct gtaacaacgg acacggaaat
     8701 gccgtctgaa cgccgaaagc cttcagacgg cattgtttga accaaatata gtggattaac
     8761 aaaaatcag
//
LOCUS       DAWGRZ010000076         8720 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000076 DAWGRZ010000000
VERSION     DAWGRZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8720
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.076"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            191..1726
                     /locus_tag="WHT44_001210"
     CDS             191..1726
                     /locus_tag="WHT44_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ2533393.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            1723..1815
                     /locus_tag="WHT44_001211"
     CDS             1723..1815
                     /locus_tag="WHT44_001211"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ2533394.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1851..3095)
                     /gene="lysA"
                     /locus_tag="WHT44_001212"
     CDS             complement(1851..3095)
                     /gene="lysA"
                     /locus_tag="WHT44_001212"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ2533395.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAALN
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRINPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYHYAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQDENVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGS
                     LLTRVEFVKYGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKDIATLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGNEYRLIRRR
                     ETLEQQMANELACLQAEHQNAV"
     gene            complement(3106..3276)
                     /locus_tag="WHT44_001213"
     CDS             complement(3106..3276)
                     /locus_tag="WHT44_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ2533396.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QSKPQSAPQTQK"
     gene            3347..3670
                     /gene="cyaY"
                     /locus_tag="WHT44_001214"
     CDS             3347..3670
                     /gene="cyaY"
                     /locus_tag="WHT44_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ2533397.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GAQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDGRDFYDVLNEALSAASGEAV
                     EIAEL"
     gene            3697..4713
                     /locus_tag="WHT44_001215"
     CDS             3697..4713
                     /locus_tag="WHT44_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEZ2533398.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAEPVTKAESHKVSVAIAVV
                     VIFGTLAIFTYPLFYTWSQHLINAHQFGIYVGSSVHEVAQVYAIGENIDPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSNGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            4739..5158
                     /locus_tag="WHT44_001216"
     CDS             4739..5158
                     /locus_tag="WHT44_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ2533399.1"
                     /translation="MAQLPLYLTSEIKDFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSRKST"
     gene            5180..5686
                     /gene="luxS"
                     /locus_tag="WHT44_001217"
     CDS             5180..5686
                     /gene="luxS"
                     /locus_tag="WHT44_001217"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ2533400.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPGKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            5832..8648
                     /gene="polA"
                     /locus_tag="WHT44_001218"
     CDS             5832..8648
                     /gene="polA"
                     /locus_tag="WHT44_001218"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ2533401.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSSETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLAGVMEHAS
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHAELSDGIESLRRTTPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEMPFEKQAEKATAPEKLDYQA
                     VTTEAQFAALLDKLSRADTIGIDTETTSLDAMNASLVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLGNPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPKDGRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTEEGRKVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSRWLSECEASPWDELLQSKLIMQVHDELVLEVVETELDFVKEKLPQIMAK
                     VDGGLLDVPLVAEVGVGENWEEAH"
BASE COUNT         2040 a         2257 c         2299 g         2124 t
ORIGIN      
        1 aaatttttcg ttttcaagcc ttcaccgctt gccatcagcg ttaaattttt ttacgataag
       61 cacatagatt gtaaacaatc ggccacaagc cggtttgttt tttcagaaga cattatccct
      121 gtcagacgct atttctatat atttcgccta taatggcttg tttttaataa ataattcaag
      181 aggtatcaac gtgtctgatt ccaagacgaa agaacgcgcc acattcggca cgcgccgcgc
      241 gtttatgatt gccgccatcg ggtccgccgt cggcttgggc aatatttggc gtttccccta
      301 tattgctttt gaaaacggcg gcggcgcgtt catcctgccc tatctggtcg cgcttctgac
      361 ggcgggcatc ccgctgctgc tgctcgatta tgccatcggc caccgttacc gtggttctgc
      421 gcccttggct ttccgccgcc tcggacgatg gtttgagccg gtcggctggt ggaacgtgat
      481 gaccaatatc gtcatctgca tctattacgc ggtaattatc ggttgggcgg caagctatac
      541 ctattattcg gtcaacgccg cctggggtgc ggatccgcag ggttttttct ttaaggactt
      601 cctgcaaatg gcgggcccgg aagccttggg tttggatttt gtcggcaaag tcgccggtcc
      661 tttggcgggc gtgtgggttt ttaccgccgc cattatggcg ttgggcgtgc aaaagggcgt
      721 ggcgcgcgcc tcgtcgttct ttatgccgct gcttttggtg atgtttttga ttatggtcgg
      781 catttcacta accctgccgg gtgcggcaaa gggcttggac gcattgttta cgcccgactg
      841 gtcgaaactc gccgattcca aggtctgggt ggcggcatac gggcagattt tcttttcgct
      901 ttccatctgc ttcggcatta tggttaccta ttcttcttat ttgaagaaaa aaaccgactt
      961 gggcggaacg gggctggtgg tcggttttgc caacagcagc tttgaactgc tcgcgggcat
     1021 cggcgtgttt gccgcattgg gctttatggc gcaggcgggc ggtaaggcgg tcaacgaggt
     1081 tgcctcaggc ggcatcggtt tggcgtttat cgcctttccg accattatca accaagcacc
     1141 gatgggctgg ctgatcggca tattgttttt cggttcgctg gtgttcgccg gcgttacgtc
     1201 gatgatttcc atccttgaag tgattgtggc ggcgattcag gacaagctga acatcgggcg
     1261 cgtcaacgcc acgctgctgg tctgcattcc gatgggcatt gtttccacgc tgctgttcgg
     1321 tacggcgacg gggctgccgg ttttggacgt gatggacaaa ttcgtcaaca cctacggcat
     1381 tgttgccgcc ggctttgttt atgttgccgc catcatcatc agcggcaggc tgccggaatt
     1441 acgcaagcac ctgaacgctt tgtcctccat ccgcatcggc ggcttgtgga cggtctgcgt
     1501 cgtggttacc gtcgtgatgc tcggctatat gctgtttaaa gataccagcg gcctgatgga
     1561 gaaaaattac gaaggttatc cggatggttt cctcagtatt ttcggctggg ggatgtcggc
     1621 ggcgttggtc gtgttcgggc tgctgctgtc gttgctgcct tggaaacacg gtcaggattt
     1681 caacgtcaaa gacgaacacg aacatgaaca aggagaagaa aaatgagtac ttccgccatt
     1741 gtgatgatga ttgtctcaat cgtgataatc tggggagggc tgctgctttc cctgttaagg
     1801 ctgccgaacg agtaagcctt tagagcgtta aaaatgccgt ctgaaccgct tcagacggca
     1861 ttttgatgtt cggcttgcag gcaggcgagt tcgtttgcca tttgctgttc caaggtttcg
     1921 cgccggcgga tgagtcggta ttcgttgccg tccaccaaca cctctgccgc acggttgcgc
     1981 gcgttgtaat tgctcgccat actggccccg tatgcgcccg cgctgcggat aagcagcaaa
     2041 tccccttctt cgcaggcgat ggtgcggtct ttgccgagga agtcgccggt ttcgcaaatc
     2101 ggaccgacga tgttggcggt cagcgtcgcg atgtctttgg tttcgaccgc ctcgatgtga
     2161 tgataggcat cataaagcgc cgggcgcatc aaatcgttca tcgccgcatc gaccatcaca
     2221 aagtttttct cttcgccgta tttgacaaac tcgacgcgtg tcagcagcga acctgcgttg
     2281 ccgaccaggc tgcggccggg ctcaagaatg agtttcagac ggcgtgtgcc gatcagtttt
     2341 tgaacggctt gggcatacgc gcccaaatca ggcacatttt cgtcttggta aacaatgccg
     2401 acgccgccgc ctaagtctaa atgttccaaa acaatgcctt cggcggcaag cgcgtcaacc
     2461 aaaatcaaaa tgcgctcgca ggcttcgacc agcgggctta agtcggtcag ttgcgaaccg
     2521 atgtggcagt cgatgccgat gattttcaaa ttgggctgtt gtgcggcata gtggtaggct
     2581 tcgagcgcgt cggcgtaggc gatgccgaat ttgttggctt tcagacctgt ggagatgtag
     2641 ggatgggttt ttgcatcgac atcggggttg atgcgcaggg agacgggcgc ggttttaccc
     2701 aaacgtgcgg caactttctg aatacggtcg atttcgggga tgctttccat attgaagcat
     2761 ttcacgcctg cattcagcgc gaactcgatt tccgcctcgc ttttgcctac gcctgaaaat
     2821 atggtttttg ccgcgtcgcc gcctgccgcc aaaacgcgtg ccaattcgcc gccggacaca
     2881 atgtcaaaac cgctgcccag cgaggcgaag tgtttgataa tgctcagatt gccgtttgcc
     2941 ttgacggcgt aacagacgag cgggttcaaa gcggcaaacg cggtttggta gtgttcaaat
     3001 gcttcggtca gcgcggattg gctgtacaca taaagcggtg tgccgaatgc ttcagcaagg
     3061 cgggggtagg ggacttgttc gcaaaatagg gtcatgtttt cgttttcatt tttgggtttg
     3121 tggagcggat tgcggtttgc tttgaagttg caaaccggtt tggattacgc cgaaacgcgc
     3181 cttgtcgcct tctttgggca ggtagaggtc gcctttgtaa ccgcaggccg agagcaggag
     3241 ggcggttgcc gccgcaaaaa atacgccgta tttcatcggt aaacttcctt cataagcgcg
     3301 aatgtggcaa gattcggcat cttaaacaaa aaacacgcaa aaagctatga tgaccgaaag
     3361 cgagtttatc cgcgcgagcg aagcattatt tgaacacatc gaagaccaaa tcgacgaaaa
     3421 cggctgggat ttcgactgcc ggtttgccgg aaacgtcctg accatcgaag ccggagacgg
     3481 cgcccaaatc atcgtcaacc gccacacgcc caatcaggaa ttgtggattg ccgcaaaaag
     3541 cggcggctac catttcgccg agcaaaacgg caaatggctg gcaacgcgcg acggacgcga
     3601 tttttatgac gttttaaacg aagccctgag cgcggcttcg ggcgaagcgg tggaaattgc
     3661 ggaattgtga tttgggtgtt atcacggaaa gaaaaaatga acacacgtcc cttttatttc
     3721 ggactgatat ttatcgcgat tatcgctata cttgctaact atttaggaaa cactgatttt
     3781 tcccatcatt atcatatcag tgctttaatt attgctatct tgctgggaat ggcaatcggc
     3841 aataccattt atccgcaatt ttcgacacaa gtggaaaaag gcgttttgtt tgccaaaggc
     3901 gcgcttcttc gcactggcat tgtgttgtat ggttttcgcc tcacttttgg cgatattgcc
     3961 gatgtaggat taaatgcggt tgtcactgat gcaatcatgc taatttcaac cttctttttt
     4021 accgcacttt taggcattcg ttatctaaaa atggataaac aattggttta tctcactggg
     4081 gcaggttgca gcatttgcgg tgcggcagca gtgatggcgg cagagcctgt tactaaagca
     4141 gaatcccata aagtttcagt ggcgattgcc gtagtggtca ttttcgggac gcttgctatt
     4201 tttacttacc ccttgttcta cacgtggtca caacatttaa ttaacgccca tcaattcggt
     4261 atttatgttg gttctagtgt acacgaagtg gctcaagtgt atgcgattgg ggaaaatatt
     4321 gatcctatcg tggcgaatac tgccgtcatt tccaaaatga tccgagtgat gatgctcgcc
     4381 ccctttttat taatgctttc ttggttatta acacgtagta atggagtatc agaaaataca
     4441 tcacacaaaa ttacaattcc ttggtttgct gtacttttta ttggtgttgc catttttaat
     4501 tcttttgatt tattaccaaa agaactcgtg aaattattcg ttgaaatcga ttctttctta
     4561 ttaatttcat caatggctgc gcttggctta acgacgcaag caagcgcaat caaaaaggca
     4621 ggattaaaac cgtttgtttt aggaatacta acttatttat ggctagtggt tggtggattt
     4681 ttagtgaact atggaatatc aaaattaata taaaattcac taaagagagc gttacccaat
     4741 ggcacaatta ccgctatatc tgacttctga aatcaaagac tttactgtcg gcacgcctaa
     4801 agttttagaa tcattttcca aacatatccc ttatggtgtc gtctttgaag acgacggcga
     4861 cacaggctac ttctatgccg cttcgcaaga cgggatttta gatgccttgc acatctataa
     4921 tgtcgaagat gtatccgaca aacatatccc caatcatgtc ttgattttat gggatgatgc
     4981 ctgcaccata gccgcattgt gtatcaacga ctacattcat gccgtctatg attttgtcga
     5041 acaggcagga tattgccgca acggcttccc tgaagcaggc ggcgaatggg tgaaagtcga
     5101 aaaccgcgtc ttggatgatg aattgctgga caaaatccta tcccgaaaat ctacataacc
     5161 ctcacaaaag gatacccaaa tgcccctact agacagtttc aaagtcgatc acacccgtat
     5221 gcatgccccc gccgtacgcg tggcgaaaac catgactacg cccaaaggcg acaccattac
     5281 cgtgtttgac ctgcgctttt gcgttcccaa caaagaaatc ctgcctggaa aaggcataca
     5341 cacgctggag catttgttcg caggttttat gcgcgaccac ttgaacggca acggcgtgga
     5401 aatcatcgac atttccccga tgggctgccg caccggtttt tatatgagtc ttatcggcac
     5461 gccttccgaa cagcaggtcg ccgatgcgtg gctggcttcg atgcaggatg ttttgaatgt
     5521 caaagaccaa agcaaaatcc ccgagttgaa cgaataccaa tgcggcactt atcaaatgca
     5581 ctcgctcgcc gaagcgcagc aaatcgcgca aaacgtgttg gcgcgcaaag tggcggtgaa
     5641 caaaaatgaa gagctgacgc tggatgaagg gctgctgaac gcctaatccg ccaaaaatgc
     5701 cgtctgaaca agggtttcag acggcatttg ccttttccgt tataatccgg ggttgtccgg
     5761 gggcgggttt taagccggca tcgtccttcc ctattttttt ctgtccctta tcggttttaa
     5821 gcgggttttt tatgtccaac agacctacac tcctcctcgt tgacggatcg tcctacctct
     5881 accgtgcgta tcacgcgatg gggcaaaacc tgaccgcccc cgacggcgcg ccgacgggtg
     5941 cgctgtatgg tgtattgaat atgttgcgcc gtttgcggtc ggaatatccg cacgattatt
     6001 gcgcggtggt ttttgatgcg aaaggcaaaa atttccgcca tcaaatgttt gaagaataca
     6061 aggcgacgcg cccgccgatg cccgacgatt tgcgcccgca ggcggaagca ctgccggatt
     6121 tagtgcgcct gacaggctgg ccggtattgg tgattgggca ggtggaggcg gacgatgtga
     6181 tcggcacgct ggcgaaacag ggggcggaac atggtttgcg agtcattgtt tcgaccggcg
     6241 ataaggacat ggcgcagttg gtggatgagc gcgttacgct ggtgaacacg atgagcagcg
     6301 aaacgctgga cattgaaggc gtgaaggcaa aattcggcgt gcgccccgac caaatccgcg
     6361 attatctcgc gctgatgggc gacaaggtgg acaacgtgcc gggcgtggaa aaatgcggcc
     6421 cgaaaacggc ggtgaaatgg ctggaagcct acggttcgct ggctggtgtg atggaacacg
     6481 cttcggaaat caagggcaaa gtgggcgaaa acctgcaagc cgcgctgccc caactgccgc
     6541 tgtcgtatga tttggtcacg attaaaaccg atgtggactt gcacgccgag ctttcagacg
     6601 gcatcgaaag cctgcgccgt actacgccga aatgggcgca gctggttgtc gatttcaaac
     6661 gctggggctt ccgcacctgg ctgaaagaag cggaatcaaa catgaatacc ggctcgaccg
     6721 atgatttgtt cggcagcgac agcatcggcg agcaggcggc tttgaatgcg gaaatgccgt
     6781 ttgaaaaaca agccgaaaaa gccaccgccc ccgaaaaact ggattatcaa gccgttacca
     6841 ccgaagcgca gtttgccgct ttgttggaca aactgtcgcg ggcggacaca atcggcatcg
     6901 atacggaaac cacgtcatta gacgcgatga acgcctcgct ggtcggcatc agcatcgctt
     6961 tccaagcagg cgaagcggtt tacatccccg taggacacag cctgaccgcc gcgcctgaac
     7021 agcttgattt acaagacgta ttaggccgtc tgaaaccgca tttgggaaac cccgccctaa
     7081 aaaaaatcgg gcaaaacctc aaatacgacc aacacgtttt cgccaactac ggcatcgccc
     7141 tgaacggcat tgccggcgac gccatgctcg cttcctacat catcgagagc catctcggac
     7201 acggcttgga cgaattgtcc gaacgctggc tcggcttgga aaccattacc tacgaatcgc
     7261 tgtgcggcaa aggcgcgaag caaatcggtt ttgccgatgt cgccatcggg caggcgaccg
     7321 aatacgccgc ccaagacgcc gatttcgccc tgcgcctcga agcgcacctg cgcgcgcaaa
     7381 tggacgaaaa acagcttgaa atgtatgaaa aaatggagct gcccgtcgcg caggtattgt
     7441 ttgaaatgga acgcaacggc gtgcaaatcg accgcgccga actcgcccgc caaagcgcgg
     7501 aactcggcgc cgagctgatg aagctcgaac aggaagccta tgccgccgca ggccagccgt
     7561 tcaacctcaa ttcgcccaaa cagctgcaag aaatcctgtt cgacaaaatg ggcatcccca
     7621 ccaaaggcct gaaaaaaacc gccaaaggcg gcatttccac caacgaagcc gtgctcgaac
     7681 agctcgcgcc cgactacccc ctgcctaaaa tcatcctgca aaaccgcagc ctggcgaagc
     7741 tcaaatccac ctacaccgac aaactacccg aaatgatttc ccccaaggac ggccgcgtgc
     7801 ataccaccta cgcccaagcc gtcgccatta ccggccgcct cgccagcaac aaccccaacc
     7861 tgcaaaatat ccccatccgt accgaagaag ggcgtaaagt ccgccgcgcc tttaccgcac
     7921 cgcaaggcag cgtcatcgtt tccgccgact attcccaaat cgagctgcgc attatggcgc
     7981 acctctccgg cgacaaaacc ctgattgccg cgttccaaaa cggcgaagac gtacaccgcc
     8041 gcaccgccgc cgaagtgttc ggcactgcgc ccgaaaacgt ctcgtccgag caacgccgct
     8101 atgccaaaac catcaacttc ggcttaattt acggtatggg gcaatacggt ttggcaaaat
     8161 cattgggcat cgacaacctt tccgccaaaa actttatcga ccgctacttc gcccgctacc
     8221 ccggcgtcgc cgaatacatg cagcgcacca aagaacaagc cgccgcccaa ggctacgtcg
     8281 aaaccctgtt cggcagaagg ctctacctgc ccgacatccg caacaaaaac gccaacgccc
     8341 gcgccggagc cgaacgcgct gccatcaacg cccccatgca gggcaccgcc tccgacctca
     8401 tcaaacgcgc catgatagac gtgtcccgct ggctttcaga gtgcgaagcc tccccgtggg
     8461 acgaactctt acaaagcaaa ctgattatgc aggtgcatga cgaactggtg ctggaagtcg
     8521 ttgaaaccga actggatttt gtcaaagaaa aactgccgca gattatggcg aaagtggacg
     8581 gcggattatt ggatgtaccg ctggtggctg aggttggcgt aggggagaat tgggaagagg
     8641 cacattgatt gaaaggtgtt atatgctatc tttatttaaa taaaatttaa tttttggtat
     8701 attttttcta aatgttccta
//
LOCUS       DAWGRZ010000077         8649 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000077 DAWGRZ010000000
VERSION     DAWGRZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8649
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.077"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(107..1030)
                     /gene="era"
                     /locus_tag="WHT44_001219"
     CDS             complement(107..1030)
                     /gene="era"
                     /locus_tag="WHT44_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ2533402.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELIKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(1078..1797)
                     /gene="rnc"
                     /locus_tag="WHT44_001220"
     CDS             complement(1078..1797)
                     /gene="rnc"
                     /locus_tag="WHT44_001220"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ2533403.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRRADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGYANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKRKKK"
     gene            1964..2287
                     /locus_tag="WHT44_001221"
     CDS             1964..2287
                     /locus_tag="WHT44_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ2533404.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            2335..2811
                     /locus_tag="WHT44_001222"
     CDS             2335..2811
                     /locus_tag="WHT44_001222"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ2533405.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            2889..3314
                     /gene="nusB"
                     /locus_tag="WHT44_001223"
     CDS             2889..3314
                     /gene="nusB"
                     /locus_tag="WHT44_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ2533406.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(3682..4716)
                     /gene="pyrC"
                     /locus_tag="WHT44_001224"
     CDS             complement(3682..4716)
                     /gene="pyrC"
                     /locus_tag="WHT44_001224"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ2533407.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDNATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENTDTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(4932..5078)
                     /locus_tag="WHT44_001225"
     CDS             complement(4932..5078)
                     /locus_tag="WHT44_001225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533408.1"
                     /translation="MPSEMRMSRPTALFLNIVCAACRFKNKPSGVQCRHFGRIAADLF
                     RNGC"
     gene            complement(5079..5303)
                     /locus_tag="WHT44_001226"
     CDS             complement(5079..5303)
                     /locus_tag="WHT44_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ2533409.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVQHK"
     gene            5412..6023
                     /locus_tag="WHT44_001227"
     CDS             5412..6023
                     /locus_tag="WHT44_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ2533410.1"
                     /translation="MRRTALLLLALAAGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSIKPHNK"
     gene            complement(6083..6955)
                     /locus_tag="WHT44_001228"
     CDS             complement(6083..6955)
                     /locus_tag="WHT44_001228"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ2533411.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEEGREEIAGNVKPTDPLKFKDSKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGIRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(6993..7778)
                     /locus_tag="WHT44_001229"
     CDS             complement(6993..7778)
                     /locus_tag="WHT44_001229"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ2533412.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAKAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSWIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            complement(8203..8295)
                     /locus_tag="WHT44_001230"
     tRNA            complement(8203..8295)
                     /locus_tag="WHT44_001230"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8259..8261),aa:Ser,seq:gct)
     gene            complement(8452..8649)
                     /locus_tag="WHT44_001231"
     CDS             complement(8452..8649)
                     /locus_tag="WHT44_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533413.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDAAL
                     ARFPEDAWFQEIKQDAQKHFA"
BASE COUNT         2101 a         2561 c         2052 g         1935 t
ORIGIN      
        1 tgcacagata atatcaaccc gtttttaaca aagatattcc cgattgaaac aaaatgccgt
       61 ctgaaacctg ttcggacggc atttgcgtaa agttcagcaa gaaaaactac aaacccagct
      121 cgcgcaggaa gcggatgtcg tccgcccaac cggatttgac tttgacccag accttcaaaa
      181 atactttggt atcaaacagt ttttccatat ccaaccgcgc ttcggtggaa attttcttca
      241 aacgttctcc gcctttaccg attaaaattg ccttttggct ttccttatcg accaaaacgg
      301 cgatatagat gcggttcaaa ccgtcttcct cttcaaactg ctccacttcg acgttcatcg
      361 cataaggcaa ttcctcgccc aaatagcgga acaatttttc acgcacgatt tccatcgcca
      421 aaaaacgcgc cgatttgtcc gtaaccatat cttcgggata catcggcacg ctttcgggca
      481 gatacggctt aatcagctcc aacaggttgg caatccgcaa tccgtgtttc gcgctgaccg
      541 cctccgccgc cgcaaattca aattcggcgc gcacttgggc aacaaacgcc tccagcgcgt
      601 aacggtcttt cgccttgtcc ttgtcgattt tgttgaccac taaaatgacc ggcgtgtgct
      661 tgggcagttg tttcaacacg acgcggtcgg catcggtaaa gcgcatcgcc tccacgacga
      721 aaaccaccac atccacgccg ccgagcgcct cggtaacatt ttgattcagc ctgtcgttga
      781 gcgcgttgcg gtggtcggtt tgaaagccgg gcgtatcgac aaacacgaac tgcgcggtat
      841 cgtcggtata aatccccgtt acgcggttgc gcgtcgtctg cgcctttttg ctggtaatac
      901 tgattttctg accgatgaga tggttcatca gcgttgattt gcccacgttc ggacggccga
      961 caatcgctac gaagccgcaa cggtatccgc cggcggcgcg ttcccctgca aggaaggttt
     1021 caatatccat atttgctttc gtatccatat gttcagacgg catattcacg cgccgcctca
     1081 tttctttttc ctcttcagcg gcagcttctc ttccagccat ttcaaagcct ctttcgccgc
     1141 ttcctgctcc gccgccttgc ggctcgtccc tttggcacgg cacacgaaac ccagttcgcc
     1201 caaatcgcag gaaatgacaa acatactgtc gttggcataa ccgatttgct cttcgatacg
     1261 gtatttcggc aaggcgaaac ggcgcgcctg caacgcctcc tgcaaagcag ttttgccgtc
     1321 ttttgcctga ttttgaaaat cggcgcgccg gacgcgatcg gcaaacaaat ggcgcaccac
     1381 cttttccgcc gtgttgaaat cggcatcgaa gctgacggca gcaaacatcg cctccatcgc
     1441 gtctgccagt atcgaaggcc gtctgaagcc gccgctcttc aactcgcccg cccccaagta
     1501 caggccgtcg ccgacattca tttccgccgc catttccgcc agcacgccct cattgaccag
     1561 acttgcccgc aaccgcgaca actcgccctc ggtcaacttc ggaaacgcgt caaacagcat
     1621 ccgcgccacc gtataattca aaatcgaatc accgacaaac tcgaaccgct cgttgtgctt
     1681 cgcatgatgg ctcctgtgcg tcaaagcctg ccgcaaaagc gaaatatcgc ggaacgcgta
     1741 gcccagtttc ttctgtatcg ccgcgtgtgc ctgctgtttc aaaacatcgt ctttcattac
     1801 cgtcttccgc aaaaaacagc ccgaatgcgt ttccatactc caaaaataca gaaacacctt
     1861 aaggctgcaa acggcgcaca ttgtaccacc aaaccgccgc cgtctgccga caacgcacga
     1921 atcgtggtaa aatccgtaag ttttccccca acgtattccc gtaatgaacg catttgcaca
     1981 agcactttcc tcggcactcg accgctgtat cgccaccaac accgtcgcca aacaaaaccg
     2041 gcccgtcggc ttcctctacc gcgaagcccc cgtttttgaa aacgacagcg gctggcgttt
     2101 cttcagcggc gacgagaccg acgaatacac cgacgatccg gacaacttca gcatcgtcag
     2161 cctcgccgac atcgccaaaa ccaaccctga aaccgccgca ctgctctccc aacccgaagg
     2221 cagcgcatgg gagcttgccg aagacggcac attccaaacc gtcgccgact ggcagccgca
     2281 agactgaaac cttcagacgg cattccgaaa cctccgaata ccaaaggaca cccaatgaac
     2341 accatcgccc ccaacctcga cggcaaacac ctccgcatcg gcatcgtaca ggcacgcttc
     2401 accaacgaaa tcggcagcga aatgctcaaa gtctgctgcc gcaccctcca agaattgggc
     2461 gtggcagacg aaaacattac cgtcgccacc gtacccggcg cgcttgaaat ccccatcgcg
     2521 ctgatgaact ttgcctcttc cgaaaagttt gacgcactga ttgccatcgg cgtcgtcatc
     2581 cgtggcgaaa cctaccattt cgagctggtt tccaacgaat ccggagcagg cgtcagccgc
     2641 gtcgcactcg actacaatat cccgattgcc aatgccgtcc taaccaccga aaacgacgcg
     2701 caggcaatcg aacggattga agaaaaagcc tcggatgccg ccaaagtcgc cgtcgaatgc
     2761 gccaacctcg tcaacctgct gctcgaagaa cagtttgaag acgaagaata acaaacaggg
     2821 cgtacaccaa tacacccttt ccgtttcaga cggcataccg tgccgcccaa ccgtttcaag
     2881 gaaaaatcat gaaaacagcc cgccgccgtt cccgcgagct tgccgtacaa gccgtttacc
     2941 aatcccttat caaccgcacc gccgcgcccg aaattgctaa aaacatccgc gaaatgtccg
     3001 actttgccaa ggcagacgaa gaattgttca acaaactttt cttcggcacg caaaccaatg
     3061 cggcagagta tatccgacaa atccgcccgc tacttgacag ggacgaaaaa gacctcaacc
     3121 ccatcgaacg cgccgttttg ctgaccgcct gccacgagct gtccgccatg cccgaaacgc
     3181 cctaccccgt cattatcaac gaagccatcg aagttaccaa aaccttcggc ggcacggacg
     3241 ggcacaaatt cgtcaacggc atcctcgaca aactcgccgc ccaaatccgc cccgacgagc
     3301 ccaaacgccg ttgatgcatc acaaatttaa agaaagttct atgtccttat ttctgctttc
     3361 tttgtttcgt cattcccacg caggtgggaa tttggttttt tgaattttgg gtatttcaaa
     3421 taaattctga aacttcaatc tcgtcattcc cgcgcaggcg ggaatccggt tttttgagtt
     3481 gctgccgttt ttgataaact ctggtcgcat cgaatttctg gattcctgcc ttcgtgggaa
     3541 tggcgaaatt tgaaaattgt gggaatttat cggaaataac cgaaacgtaa caaactcaaa
     3601 cgccgttgac gtatcaatca gtcagcaaca caaatgccgt ctgaaagccc tcccttcaga
     3661 cggcattttg tttccagccc atcaatactg caccgtccag ccgatttcgc cgcccgcgcg
     3721 catcgggaca agttcgccgt cgccgtaggg aacacttgcg ggaacggttt ggctttgttt
     3781 gacgagggtg atcgtgtcgg tattttcagg aatgccgtag aaccttgcgc cgttttttga
     3841 ggcgaaggct tcgagtttgt ccaacgcgcc tgctttttca aatacttcgg cgtaaagctc
     3901 gatagcggtc atcgcactga acataccggc gcagccgcag gcgttttctt tggcggattt
     3961 ggcgtgcggc gcggagtcgg tgccgaggaa gaatttatgc gccttctcgc cggtaacggc
     4021 ggcgaccaat gcctgacggt gggtttcgcg tttgagtacg ggcaggcaga aatgatgggg
     4081 gcgcacgccg ccgaccaaga ggtcgttgcg gttgagcagg aggtgttgcg gggtaacggt
     4141 ggcggctacg ttgtcgcctg cttccaaaac caggcgggcg gcttcggcgg tggtgatgtg
     4201 ttcgaacacg actttaagat tcggcacttg cgccaaaacg ggtttcatca cgcgcccgat
     4261 aaaggcggct tcgcggtcga agatgtcgat ttcggggtcg gttacttcgc cgtgaacgag
     4321 gaacaaaatg ccctgtttcg ccatttcttc caacacgggg atgagcttga acaggtcggt
     4381 tacgccggaa tcggaattgg tggtcgcgcc ggcagggtag agtttgaagg cgacgatgcc
     4441 ggcggctttg gcttcgcgta caagttcggg cgtggcgtta tcagtcaaat aaagcgtcat
     4501 caacggctca aacgcgctac cttcgggcaa cgccgccata atgcgcgctt tgtaggcaag
     4561 cgcgtcggct acactgacga caggcggttt gaggttgggc ataatgacgg cgcgccccat
     4621 ttggcgggcg gtataagggg caacggcttt gagcgcgtcg ccgtcgcgca ggtgcaggtg
     4681 catatcgtcg gggcggataa tggtcagggt ttgcatggtt gttccttttt aggttttggc
     4741 tgcgtttgaa tacccggtcg attccatccg tttgccgggg gaaagccggt tattccgtcc
     4801 ggtcggaaac cggttccatt ggatgaaagg cattttgtcc gactgattgg gttgcatcaa
     4861 cggaatcttc aaacacagcc tttagatttt gattgtgctt gggttttgct tctttgccga
     4921 cggcaaggcg gttaacagcc attccggaac aaatcggcgg cgatacgccc gaaatgccga
     4981 cactgcacgc cggacggttt gtttttgaaa cggcacgcgg cacatacgat attcaaaaac
     5041 aacgccgtcg ggcgggacat ccgcatttca gacggcattt atttgtgttg gacgaccagc
     5101 gtgaacacgc cgtcctgttc ggaagcatcc aacagcacat gaccggtttg gcggcaaaaa
     5161 gcctcaaaat cccccggtgc gccgccgtcg gttgccagaa cggtcaacac gtcgccctgc
     5221 tgcatttgcg ccaaagcctt tttcgcccgc aaaatcggca ggggacattt caatccggtt
     5281 acgtctaaag tttcgctgtt catcgtttgt accgtttaaa aaatgggaat cggggggaga
     5341 ttatacccca gttgcttgca gccccctttg ccaacccata aaataacgcc gttttcaatt
     5401 tggagaaact tatgcgccgc accgccctcc tgctgctcgc actcgccgcc ggcacgtccc
     5461 tcgctgccgg cttcagccaa aaagacacgc cgatcaacac ccgctaccgc gaaacgcccg
     5521 aagaggccgc cgcccgcgaa tttaaagaac ataccgccaa actgccgccg ctgcccgata
     5581 cgcactcgga cggctggttc gacatttatg tggatgaaaa ttacggcaaa cagccgaaaa
     5641 tcctgctcga cagcctgcaa attatgcccg cgcccgacgg cagcatccgc tacattctca
     5701 atatccgctc cgacaaaggc tacgacaacc tgactgccga aggcatattc tgcgcccgct
     5761 tctccatcag gttcggcaac ggcaaacctt cctcctataa agtgttcggc tacggcgaca
     5821 cggtcaaccg ccgctggata cagccccgaa acgccgaatg gaaaccgata ggcggtacat
     5881 taagccgcaa cgacgcactg cgcaccgtcc tgtatcaggc attctgcgag ggcggtgtac
     5941 ccgccgacac gaaaggtttg gtacaacgcc tgaaagaacg tgccggacgc tatgccccgt
     6001 cgataaagcc gcacaacaaa taaccagaca caaaaatacc gtctgaaaca tcggtttcag
     6061 acggtattcg attcatcagc catcaagcgg cggaaacttt gtcctgacgg cacaacagcg
     6121 taatcaaatc actgatgcgc cgcttcatat cgcggcggtc gacaatctgg tcgattgcgc
     6181 ctttttccag caaaaactcg gcgcgttgga agccttccgg cagcgtttcg cgcaccgtct
     6241 gctcaatcac gcgcggaccg gcaaagccga ttaatgcatt gggttcggca agcacgacat
     6301 cgcccaaaaa tgcgaagctg gcggatacgc cgcccatagt cggatcggtc aacaccgata
     6361 taaatggcag gcgtttttcc gtcagcaaat gcagcgcggc actggttttc gtcatctgca
     6421 tcagcgagtt tacaccctcc tgcatacgcg caccgccgga agccgccaca cagacgaacg
     6481 gacaattgtc ggcaaccgcc cgacggatac cttgtacgaa tcgttcgccc acaaccgaac
     6541 ccatcgaacc gccgataaag cggaattcaa acgcggcaac gacgacgggc agaccgttca
     6601 tcttgccttt cattaccacc aaagcatcat cttccccggt cagcttgcgt gccgcactca
     6661 aacgatccgg atattttttg ctgtctttaa acttcaaagg atcggtcggt ttgacatttc
     6721 cggcaatctc ctccctgcct tcctcatcca aaagcagatt caggcgttgt cgtgccgaca
     6781 acggattgtg gtggttgcat ttcggacaaa cctgattgtt ctgctgcaac tcggtcgaat
     6841 aaacggttgc cgaacaagac ggacatttgt gccacaaacc ctcgggaaca ttggaagaac
     6901 cgtctttgct gcggtttttg attttgggtg gcaggatttt atctaaccag ctcatggaca
     6961 actcctaaaa tattcaggat gaggaaccgc cgtcagcgca cggcatcctt caactctttg
     7021 accaaagcac cgacggccgc agcctcgttg cccgcattgt tttcaatctc tttcacaatc
     7081 cagctgccga caatgaccgc gtcggcaacc cggccgattt tgcgcgcgct ttccgcattg
     7141 ctgatgccga aaccgacacc gatgggaata tcgatatact gatgcaaata ctctatttta
     7201 cgcgaaacct catccgtatc caaacttgcc gcgcccgtta cgcccttgag cgaaacataa
     7261 tagacaaatc cgcctgccag ctcggcaatg gttttaatac ggtcttccgt cgtcgtcggc
     7321 gcaatcagga aaatacagtc gaccccgtta tccttcagct cgcgatagag cggatcgatg
     7381 gtttcgacag gggaatccac cgtcaacacg ccgtccacac ccgcctttgc ggcttcctga
     7441 gcaaactccc gataacccat cttatgtaca gggttcaaat atcccatcaa aacaaccggc
     7501 gtttgcgtgt cggtttcacg gaattttctg acgacatcca agacatcgcg cagcgaaatc
     7561 ccgtttgcca acgcccgctc cgccgcacgc tgaataaccg gcccatccgc catcggatcg
     7621 gaaaacggca cacccaactc caaaatatcc gcaccgtttg caaccatgcc gtgcatcaat
     7681 gccaaagttg tccgaatatc ggggtcgccc accgtaatat agggaatcaa tgcctttccg
     7741 ccatccaaag cggcaaaagc ctgccggatt ctgctcattt gtctcttcca tcctatcggc
     7801 aaatatgcca taaaacacaa tcagtaaatt tttcccagta taacatttgc ggtacggata
     7861 ggcgaatacg gcaaacagaa accacgcaaa tcagagccgc cgtttgaacg aggcggaaat
     7921 aaacggcaag catctcggat tatagtggat taacaaaaat caggacaagg cggcgaagcc
     7981 gcagacagta caaatagtac ggagccgatt cacttggtgc tccagcacct tagagaatcg
     8041 ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttaatcca ctataacaaa
     8101 attcagaatc aagacagggc aataccatac tggttttgca ccaacccgct ataaaattgc
     8161 ataaaaaata gacgctgtaa aacacagcgt ctatttttta tctggcggag taagagggat
     8221 tcgaaccctc gatgcaggtt gaccccacat gcttccttag cagggaagtg ccttcagcct
     8281 ctcagccatt actcctaagg aagctgtaac tataatcaac ttttccgata ccgtcaagga
     8341 aaaatggaaa gcaaatatta atcttttgaa ataaaataag atttaccctg attataccca
     8401 atcaggcact tcttttcttc ctgaatggaa atattcctga ctgccacatc ctcaagcaaa
     8461 atgcttttgt gcatcctgtt tgatttcctg aaaccacgcg tcttcaggaa aacgtgcaag
     8521 cgcggcatcc aatgttgaca gtaggttttg cgacagggct ttggtcaggt attcttcctc
     8581 gttggcttct gccgctttgt cgggctgttg tgccaaaccg tcggcacggg ctttgtgtcc
     8641 gtgtgccaa
//
LOCUS       DAWGRZ010000078         8621 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000078 DAWGRZ010000000
VERSION     DAWGRZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8621
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.078"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            106..354
                     /locus_tag="WHT44_001232"
     CDS             106..354
                     /locus_tag="WHT44_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEZ2533414.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLIGIEKP
                     ARNAGRLKKFSHMENTDISRILEDDNETAALFFEESAL"
     gene            351..737
                     /locus_tag="WHT44_001233"
     CDS             351..737
                     /locus_tag="WHT44_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEZ2533415.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGISARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(917..2272)
                     /gene="yegQ"
                     /locus_tag="WHT44_001234"
     CDS             complement(917..2272)
                     /gene="yegQ"
                     /locus_tag="WHT44_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ2533416.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVADMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLTGHSTAKQSQYVGHVTEIDENGWATVEVKNR
                     FAVSDSLEIIHPSGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMQGKEKALIARVLN
                     P"
     gene            2597..3385
                     /locus_tag="WHT44_001235"
     CDS             2597..3385
                     /locus_tag="WHT44_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ2533417.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAEAGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALKHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEREGLKTVRWHEEAVDLLPAGASKTDGIRSVVEALGWEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPSVDEDGVLRGLQDLGVI"
     gene            complement(3431..4030)
                     /locus_tag="WHT44_001236"
     CDS             complement(3431..4030)
                     /locus_tag="WHT44_001236"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ2533418.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKLGSRVLLVDDLIATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            4158..4775
                     /gene="gmk"
                     /locus_tag="WHT44_001237"
     CDS             4158..4775
                     /gene="gmk"
                     /locus_tag="WHT44_001237"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ2533419.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTGAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4834..5040
                     /locus_tag="WHT44_001238"
     CDS             4834..5040
                     /locus_tag="WHT44_001238"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ2533420.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            5132..7288
                     /locus_tag="WHT44_001239"
     CDS             5132..7288
                     /locus_tag="WHT44_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ2533421.1"
                     /translation="MPAPQPSAPYDPLTAEARALLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(7355..8539)
                     /gene="coaBC"
                     /locus_tag="WHT44_001240"
     CDS             complement(7355..8539)
                     /gene="coaBC"
                     /locus_tag="WHT44_001240"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ2533422.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILKGKKVLITAGATFEAIDPVRGITNISSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAENMHRAVHRLIDKQDAFISVAAVSD
                     YRVKNRSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMTYAR
                     EKRIKKKLPMIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMRIVERLAVYL
                     SK"
BASE COUNT         2145 a         1991 c         2324 g         2161 t
ORIGIN      
        1 caaggtgtga ataaaacggc ggatataatc aaatatatgt aatgagattc ccaaaatctt
       61 ggtaatgatg tatattagtc aagttagcta atcatgagaa gtgttatgtt tcaagcaaat
      121 attcatcagg caaaaaccaa tttgagtcaa ttgattcaaa gagcagaagc aggggaaatc
      181 gttattattg cgaaggcagg taagccttgc gtccaactaa tcggtattga aaaaccggca
      241 cgaaatgcag gaaggttgaa aaaattcagt catatggaaa atacggatat ttcacgtatt
      301 cttgaggatg acaatgaaac ggcagcttta ttttttgagg agtcggctct gtgagaaaga
      361 ttctgcttga tacccatgcg ttgctgtggt ggttgttgga tgacaagaaa ctggggatat
      421 ctgcacgcaa actgatagaa aatccgagaa atgcgatctt tgtcagtgca gcaagtattt
      481 gggaaatttc catcaagcag aacaaggggt tgttgaaatt accggaagag ttttttgatg
      541 tgcttcaaga agaggatttt gaaatgttgc ctataggtct gtttcatgca aaacaggctg
      601 gaagccttcc ggagatacac aaagaccctt tcgataggat gttgattgca caaacccaag
      661 cggaaggctt tgaactgatg actgtcgatg aatatattcc gcaatatgga attagagttg
      721 tcaacgcatc aagctgacaa atacaaaaac tgtgtacaca agaaagaccg ccgaggcggt
      781 cttttcggtt attgtcttcg cttaaaagcc agacttctgt cgtggactat aggtgtgtcc
      841 cgatctgaat ctgccgcaca ataaggcaag gggattaaaa atgccgtctg aaaaatgttt
      901 cagacggcat aatggcttag gggttcaaca cgcgtgcgat gagggctttt tctttaccct
      961 gcatattggg gattttgacc tgaatgccgt tgcccggggc aacatcgaca ggctggcctt
     1021 tgcgggtcat ttgttccaat ttgatggttt ggttgccgct cgggtggatg atttcgagtg
     1081 aatcgctgac ggcaaagcgg tttttgactt ccactgttgc ccagccgttt tcatcgattt
     1141 cggtaacgtg tccgacgtat tggctttgtt tggcggtgga atggccggtc aggtagtttt
     1201 gataatcctg agtttggtgg cgttcgagga agccgctggt gtagccgcgg ttggcgaggc
     1261 cttcgagttc gctcaacagg ctgtaatcaa acggacggcc tgcgacggca tcatcaatcg
     1321 ctttgcggta ggactgggcg acgcgtgcaa cataatagag cgatttggta cggccttcga
     1381 ctttgaggct gtccacgccg attttggcga gtttttcgac gacttcgata ccgcgaaggt
     1441 ctttggaatt catgatgtag gtgccgtgtt cgtcttccat aatcggcatc atttcgcccg
     1501 ggcggttgga ttcttcaatc aggaaaactt tgtcggcgta gggatggcgt ttttgaccgt
     1561 tgatgccttc aaagttttgg ttggcttctt cttgggcttt ttcaaagttg aaaccttgca
     1621 gaagctgggc atcgcctgca tcgctttccg tggcattgtg aaccttgtaa tcccaacggc
     1681 aggagttggt gcaggtgcct tggttggggt cgcggtggtt gaaatagccc gacaataggc
     1741 aacggcctga ataagcgatg cacaatgcgc cgtggatgaa gacttcgagt tcgatgtcgg
     1801 ggcattcttg gcggatttcg gcgatttctt ccatactcaa ttcgcgcgac agaataatgc
     1861 gttcgacgcc gatgttttgc cagaatttca cgccccaata gttggtggtg ttcgcctgta
     1921 cggacagatg gatcggcatt tccggccatt tttcgcgcac ggtcataatc aaacccggat
     1981 ccgccataat cagcgcgtcg ggtttcatgg caatcagcgg ctccatgtcg gcaacgaagg
     2041 ttttgagttt ggaattgtgc ggcagggtgt tgacggttaa aaagaatttt ttgttgcgct
     2101 cgtgcgcttc tttaatgcct tgttctaaaa catcaagttt ggcaaattcg ttgttgcggg
     2161 cgcgcagtga gtaacgcggg ctgccggcgt aaacggcgtc tgcgccgtag tcgtaggcgg
     2221 cgcgcattct ttccaatccg ccggcgggca ataagagttc gggtgctttc atcgtgtttc
     2281 cttttcggtt gaaaccccgc cctttagggc agtagaatca gactttattt gggaggggcg
     2341 taaccccttc cgaatcaggg caacatatag ggcgacgctt tatgtgtcgt cctgtgtgtt
     2401 gaaacatgat ttgtatgctt tgtaaaaatg tttcagacgg cattgctgca aatgccgtct
     2461 gaaaagggtt tgaggatggg cggattatcc gcctttttgt attttcggtc aattttgacg
     2521 ggaaatttat aaggatttat caagtatttg cctgcttggg atataatgcg gtagtttgaa
     2581 tgcgagagag aatgtgatga atcctaaaat cgtgtttttc gacattgacg atacgctgta
     2641 ccgaaaatat acggatactt tgcgcccttc cgtgaaaacg gcggtggcgg ctttgcgcgg
     2701 caaaggtata ttgacggcgt tggcaacggg gcggtctttg gcgacgattc ccgaaaaggt
     2761 cagggatatg atggcggaag cgggaatgga tgccgtggta acgataaacg gacagtttgc
     2821 gctgctgcac ggaaaaaccg tgcgcgaagt accgatggat gccggtttga tgggcagggt
     2881 ttgcgcgcat ttggatggct tgggcatgga ttatgcgttt gtcggcggag aggggatcgc
     2941 tgtgtccgcg ctgtcggaat gcgtgtgccg cgccttgaag catatcgcca gcgatttttt
     3001 tgccgataag gattattttt caagcaaacc ggtgtatcag atgctggtgt ttgcggagga
     3061 aaacgaaatg ccgctttggt cggatattgt ggaacgggaa ggcttgaaaa cggtgcgctg
     3121 gcacgaggaa gcggtcgatc tgctgcctgc gggtgcgtcg aaaacggacg gcatcagaag
     3181 cgtggttgaa gcattgggat gggaaatggc agacgtgatg gcgttcggcg acggtttgaa
     3241 cgatgtggaa atgctgtcag aagtcgggtt cggcgtggca atgggcaacg gggaacaggc
     3301 ggcgaaagaa gcggcgaaat atgtttgccc cagcgttgat gaagacggcg tgttgagggg
     3361 cttgcaagat ttgggcgtga tttgaacgca tcataacaac cctgccggtt tcagacggca
     3421 gggtcggttt tcagcccttc atacagcctt cgttttgaag cagggtaaat aagggcgcgc
     3481 cgcttgcacg gatattcttg ccgccttgaa ggtcggtaaa ttccaaaatg gcggcggctt
     3541 cgacaatttc tccgccgagt ttgcggatca gttccagtcc ggcaagcatc gtgccgcccg
     3601 tggcaatcaa atcatcgacc agcagcacgc gcgaaccgag tttgacggca tcggtgtgga
     3661 tttccaccgc agcttccccg tattcgagcg cgtagctttg cgatacggtt tcaaaaggca
     3721 gcttgccttt tttgcggatg gggacgaaac cgacgttgag ctggtaggcg agtgccgcgc
     3781 cgataatgaa gccgcgcgcg tccaaaccgg caacgatgtc gattttctga tccatatagc
     3841 ggtaaaccaa taaatcaacc aaaaggcgga agtattccgc gctttgaagg acgggcgtga
     3901 tgtcgtggaa taagatgcct ttttgcggcc agttttcgat tttgcggatt ttgtcggcaa
     3961 gcgcgccgac actcatagct tcgggatgaa ccagcattgc gtgttccaag tttgatgttt
     4021 ttaacgccat attgtagccg tcagcctgtc gcttgccaaa tcaatcaaaa caatagcggc
     4081 aaaatatggg catcggcaaa taggaaactg ggtgtccatt gtgatacaat cggctggaaa
     4141 atatacaggt aaaaaatatg tccgcttatc gaaaaggcaa catctttatc atttcggccg
     4201 cttccggcac gggcaaaacc acgctggtgt cgcggctgtt ggcaaaccat aacggtttgc
     4261 gcgtttccgt gtcgcacacg acgcgcccgc cgcgtgaagg cgaagcaaac ggcgtacatt
     4321 atcactttgt ttccaaagaa gagtttgagt cgcttatcgc gcaggaagct tttttggaat
     4381 acgccgacgt atttggcaac tattacggca caggcgcgga gggtgtgaat gcgttggcgg
     4441 cggcaggcta tgacgtgatt ttggaaatcg acgttcaggg cgcggcgcag gttcgcgacg
     4501 cgctgcccga agccgtcggc atctttatcc tgccgccttc tttcgacgta cttgccgcgc
     4561 gcctcaacgg acgcgggacg gacagtcggg aagttatcca aaggaggctg tcgaaggcaa
     4621 ggcatgaaat cgagcagtcc gtattgtttg actttgtcgt ggtcaatgac gacttggcgc
     4681 gagcggagga ggatttgcgc catattgtga atgcctgccg tctgaaaagg tcgcggcaac
     4741 tggggtttat tgcagatttg ttggaaaatt cctagaaaac ggcgaaaata cccggtttcc
     4801 caatttaaat atttttgaaa gaaagcaaat aatatggcac gtattaccac cgaagactgt
     4861 accggaaaaa tttccaacca ttttgacctg acattggtag cggctcgccg cgcccgccag
     4921 cttgagaacg gcaacacgcc gcttgtggac gatgtccgca ataacaaacc gaccgttacc
     4981 gccttaaggg aaatcgccgc cggacatatc ggtacagaac tgttgacgcg caataaataa
     5041 attctgccgg aaacgcacgc cggaacactt tgccgccgtg cagtccgacg gtttgaaatg
     5101 aaaaacacat acataccgaa agccgtctga aatgcccgcc ccccaacctt ccgcccctta
     5161 cgaccccctg accgccgaag cgcgtgccct gcttttccat accgcctcct acctcaagcc
     5221 cgaggaacag gcggagcttg aaaaagctgt cgcctatgcg tttcgcgccc acgacgggca
     5281 aacccgcaaa agcggggagc cctacatcac gcatccgatt gccgttgcga cgcagctcgc
     5341 cctttggcat atggacatac agggtctttg tgcaggcgtg atgcacgacg tattggaaga
     5401 tacgggcgtg acaaaagggg aaatggcggc ggtgttcggc aatacgattg ccgagatggt
     5461 ggacggtctg tccaagcttg aaaaactcaa atttgaagat catgcggagc atcaggcgga
     5521 gagtttccgc aaactgattt tggcaatgac caaagatgtg cgcgtgattg tcgtcaaact
     5581 tgccgaccgc ctgcacaata tgcggacgct cggttcgatg cgcccggaca aacgccgccg
     5641 gattgcaagg gaaacccttg aaatctatgc acagattgcc aaccgtatag gtttgaataa
     5701 cgcatatcaa gagcttcagg atttatcgtt ccaaaacctg catcccaacc gctacgagac
     5761 tttaaaaaaa gcgatggaca agagccggaa gaaccggcag gacgttgtcg gcaaagtctt
     5821 gcgcgcattc ggccagcggc tggtaggcgc gaatatagag gccaaaatca aaggcaggga
     5881 aaaaaacctg tacggcatcc atcagaaaat gatggcgaaa aagctgcgct ttgccgaggt
     5941 tatggatatt tacggtttcc gcgtcattgt caacagcatt ccagcctgtt atgccgcact
     6001 cggcgcattg cacaccctct atcagcccaa gcccgggcgg ttcaaagact atatcgccat
     6061 tccgaaaagc aacgggtatc aaagtctgca tacgactttg gtcggccctt acggcttgcc
     6121 gattgaagtt cagatacgta ccaaggaaat ggatgctgtt gccgaaggtg gaatcgccgg
     6181 acattggagc tataaatcat attctaagac ggtcgatcag gcggtgcttc acacaaaccg
     6241 gtggctgaaa aatatcttag atttgcaggc aagcagtgcc aatgccattg agtttctcga
     6301 acacgtcaaa gtcgatttgt ttccgaacga aatctacatc cttacgccaa aaggaaaaat
     6361 cctaactttg cccaaagggg caacgcctgt cgattttgct tatgcggtgc ataccgatat
     6421 cgggcacaaa accgttgccg cacgtatcaa caatatcatg atgccgttgc gtacgaagct
     6481 caaaaccggt gattctgttg aaattatcac atccgaacac gccaaaccca atcccgcgtg
     6541 gttgaatttc gccgtgtcag gcagggcgcg cagcgccata cgccaatata ttaaaaacct
     6601 taaccggcac gatgcggtcg ttttgggaga gagcctctta caaaaagccc tgtccagttt
     6661 gctgcccaaa gatgtcctgc tttcagacgg catcaaggaa aaatatcttg ccgatctcaa
     6721 cgacaagcag acatcgtttg aagaagtgct gtacaacgta gggatggggc ataccctgcc
     6781 tgtttatgtc gccatgcaca ttgccgagtt ggcaggggag catttcggca gcgaggtcag
     6841 gctcagttcc attaaagtcg atgggcagga aagcgggcat attcattttg cagagtgctg
     6901 ccaccctgtt cccggcgatt ccatccgttt gctgttggtt aagggaaaag gcatgattat
     6961 ccatagggat acctgcccga cgttgttgaa gtccgatccc gaacagcagc tggatgcaga
     7021 ctgggaaaat atgaacgggc agaactaccg tgtcgggctt caagtccaat cggaagacag
     7081 ccacggcctg ttggcattaa tggcgcaagc gatttccgat tccggtgcag acattgagtc
     7141 ggtcgaaaca ccgtctaaat cccagtcggg aacggaaggt tttgtcgaat tcaaattctt
     7201 attgaaagtc aagaatctga atcaattgaa tcagattatt caaaatctgc atagtattcc
     7261 atatatccgc aaagtcatca gaagttgaag caggtaaaac gggttgcctt ttgattaaca
     7321 ggcaacccgt tttatggttt atccgttcaa ttgcttattt gctcaaatat acggcaagcc
     7381 tttcaacaat ccgcattgcc gcttcatctt tacttgtttc gggaaaagac agttccccgt
     7441 cgtccccgat aatggtaatc cggttggtcg gtttgcccat tgcgattgaa acatcattgg
     7501 caacgatcat cggtagcttt ttcttaatac gtttttcccg cgcatatgtc attacattct
     7561 ccgtttcagc ggcaaaaccg atgcagaacg gcgggttcgg taatgaggca atagaagcca
     7621 aaatatcggg gttctcatcc aattcgatgg ataacggttt ggcattttta tcttttttga
     7681 atttttgagt actcctattc ttaaccctat agtctgagac ggcggcaaca gaaataaaag
     7741 catcttgttt gtcgattaaa cgatgcactg cgcgatgcat attttcggca ctgacggctt
     7801 gaaccgtatc ggatatgccg aaaggcagcg cggtttgaag ctgtccgtga atcaggctga
     7861 cttctgcacc ggcggcacgg cacgcccgcg ccaaagccac gcccattttc ccgctggaga
     7921 tatttgtgat gcctcggaca gggtcaatgg cttcaaatgt cgcacctgcg gtaatcaaga
     7981 cttttttgcc ctttaaaatt ttcggtgtcc ataaatccgg aagcagatcc agcaattcgg
     8041 caggttccgg catccttccc ataccatttt ctccgcaagc ctgttcgccc aagcccggca
     8101 tatagacagt aatgccgtct gaaaccagtt gtgcgatatt ccgttggttg gcagggttga
     8161 gccacatttc cacattcatc gcgggcgcga tggcaagcgg acatttccgt gcggctgcca
     8221 gactggtcag taggttatct gccacgccgt tacagatttt tgccacggta ttcatacttg
     8281 ccggcgcaat cagaaaaaca tccgcattcc gggtcaggtt gatatgttcc ataccgtttg
     8341 aaccgttgcc gccgtgcgtg tcggtcagga caggattgcc gcttaaagcc tgaaaagtca
     8401 gcggagaaac aaattcagtt gccgagcggc tcataaccac cgtaaccgaa tgcccctgtt
     8461 ttttcagcag tcgcaccaac tcgcaagact tatacgccgc aatactgccc gttacaccta
     8521 aaagaatatg tttgcccatt ttgacaattt ctaattacga agaaaaaaca gcagccattc
     8581 tataacaaag tacggataca ttagataccg tttggataca a
//
LOCUS       DAWGRZ010000079         8598 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000079 DAWGRZ010000000
VERSION     DAWGRZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.079"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(391..825)
                     /locus_tag="WHT44_001241"
     CDS             complement(391..825)
                     /locus_tag="WHT44_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ2533423.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WHT44_001242"
     CDS             complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WHT44_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ2533424.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1556..2398)
                     /locus_tag="WHT44_001243"
     CDS             complement(1556..2398)
                     /locus_tag="WHT44_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ2533425.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPVPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPGNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2653..3291
                     /locus_tag="WHT44_001244"
     CDS             2653..3291
                     /locus_tag="WHT44_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ2533426.1"
                     /translation="MFQNFDLGVFLLAVLPVLLSITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3743..4543)
                     /locus_tag="WHT44_001245"
     CDS             complement(3743..4543)
                     /locus_tag="WHT44_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ2533427.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(4679..5182)
                     /locus_tag="WHT44_001246"
     CDS             complement(4679..5182)
                     /locus_tag="WHT44_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533428.1"
                     /translation="MDAFDFFMQGNFKKIATEQGLDRKYFIFQYIVCNLSAISSFCLM
                     KIIHTYTILYILIAVIGIINGFLLRSKVLSIVKNKQKFLSDMFPLEMPFFFVEYKIPI
                     LARFLNRGMKWLYLPSFFCLVAICFSIYILNEEGGWMPYFACYWFGLCYPVSTYYVFL
                     SRDYKHI"
     gene            complement(5519..6160)
                     /locus_tag="WHT44_001247"
     CDS             complement(5519..6160)
                     /locus_tag="WHT44_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ2533429.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMTMSEKSGVPLYFDRAL"
     gene            6303..8516
                     /locus_tag="WHT44_001248"
     CDS             6303..8516
                     /locus_tag="WHT44_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ2533430.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKNLIGTAWLLA
                     QEHYPADAATPYGEPLPDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQKPE
                     KYREIALLLDEKRTERLEYIENFLNILRGELKKYNVHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIIGFVTR
                     ERGISVHRKTCPSFQHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
BASE COUNT         2264 a         2339 c         1870 g         2125 t
ORIGIN      
        1 ggtatagcgg attaacaaaa accagtacgg cgttgcctcg ccttaactca aagagaacga
       61 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctacggc
      121 ttcgttgcct tgtcctgatt tttgttaatc cactataaaa attagaaatg cacattttca
      181 ttattctcgc gcaggcagga ctccagactt acccatttca gtaatgtttg aaaataaaag
      241 aaaaatcaga tgtttgtatt cccgcctgcg cagaaatgga gacggtgctc tgtcgtctca
      301 tttttgtttt aatcaactat atatagctga ttaaacataa gaaatgccgt ctgaaagact
      361 ttcagacggc attcgttcaa gcgtcgaact ttattgcgcc ttggtttcgg ttacaaaacc
      421 gattttggtg attcctgcct gacgggcggc ttctaaagct ttgtttacat aatcgtattc
      481 caccgccttg tctgccgcaa tcgccacaat cacgttttca ttctgctcct tggcggcttt
      541 cagacggctt tccacttccc cgatttccac tttgcttgca gaatccccgc cgacataata
      601 gccgccgttc gcatcaatcg tcaggcgcag ggggtcttta ggctgtttgt cctgcttgtt
      661 tgtctgctcg gacgcggtcg gcagttccaa agggatggaa tgcgtcagca ccggcatagt
      721 aatcataaac acaatcagca acaccagcat cacgtccacc aacggcgtaa cgttgatgtc
      781 ggacatcgga gaatcgtcgc cggaattcat cgaaccaaat gccataatca gctatccttt
      841 tgattaagca ggcggacgtg caaatcgtgc gccatcgcat ccaaatcctg ggtcagtatt
      901 tttgtgccgc gattgaggaa gttgtatgcc aacaccgccg gaatcgccac gaacaaaccc
      961 gccgccgtcg ccaccagtgc ctcgccaatc gggccggcaa ccgccgcaat actcatctgc
     1021 ccgctttgcc cgatattgat cagggcgtgg taaatccccc aaaccgtgcc gaacagcccg
     1081 ataaacggcg cggtcgcgcc gatggaggca agcgcggtca tcccgtaatc aaaccggcgc
     1141 ataatctgcg ccatactgtt gcggatttga atgaccaaat actcgttcaa cggcaaagcc
     1201 tgcgccagtt cggacgcttc gtttcggcgg tagttgcggt aagactgcaa tgcctcttgc
     1261 gccagtttgg acaaaggcgc atcgacggcg cgcacttttt cgaccgcgtc gttcagcgac
     1321 aaagtatcgc gcatatgccg tttgacggcg gcattccctt tgcgcgcccg atacagcttg
     1381 atgcagcgca agacaaccaa acaccacgtt acgatactca tcaacagcat caacacaaac
     1441 acaccaatca ggacgggatc gcccgattca aacactaatt tcaaattcat aatgattcca
     1501 acactgaaaa aaccaatcaa acatccaagc tgccgcaaac cgctgcggca accgcctaat
     1561 tcaattcaaa cttgacgggg actttaaact ccgtccaggc attggcttga aaatgcccgt
     1621 tttgcgccgc cttgcgtgcc gcattgtcca accgggaaaa accactgctt ttcacgattt
     1681 taacggactc aacatgaccg cccggagaaa ccaaaacgct caaaacaacc gtaccctgct
     1741 cgtcattctc catagaaagc gtgggataag ccgggcgcgg aatgctgccg ttggcgcgta
     1801 aaggattgcc tttgctgctg ccggctcctt ccccgtgttc gcctttgaca ccgccgctac
     1861 ctttaccgct gccttctccg cgccccgttc cgtctccttt ggtaccagtt cccttatctt
     1921 ccccattgcc ctgctcgctg tctgctttgg cagaagcatt gccgggatgt tcggcaggtt
     1981 tttcagacgg cttctcgacc ggtttttccg ccggtttcgg gacaggcttc gcttccggct
     2041 taggctctgg tttcggtttt tcttcgggtt tcggcttttc ttcaggtttc ggctcttcct
     2101 taggctgctg aatatccgca tccgcctttt tcgtaaccac cggcttcaaa accggcttgg
     2161 gcggctcgac aggtttgggc ggctcgggca cgggttgcgg ttcgggcgca gcaggcgcgc
     2221 ctgcaccttc gggggcgccg tcccctccgc caaaatcgcc caaatcgaca aattcaataa
     2281 cattgcctga ctctatcacg ggcagcttgt gcgcctgcca gagcaatgcc accattgcca
     2341 aatgcagcag tgcgacggaa aacacgactg cgggggttaa aattcgttct ttatccataa
     2401 ttcgggcata ataatagcaa caattcctat ttgcaaccta tttttacaat ttttggtcat
     2461 atgaatgtct gttccgttca caggcaaacc gtgtttaaac gctgtattac agcaaatcat
     2521 cagataacgg gccggcagaa aaaatgattc cgtctgattt cttattccaa taaaatcagg
     2581 ttagatgata tattgccgct tctgtctgtc agccgtttcg ggctgcacac cacatctgtt
     2641 caaaggaaaa ccatgtttca aaattttgat ttgggcgtgt ttctgcttgc cgtcctgccc
     2701 gtgctgctct ccattaccgt cagggaggtg gcgcgcggct atacggcgcg ctactgggga
     2761 gacaacactg ccgaacaata cggcaggctg acactgaacc ccctgcccca tatcgatttg
     2821 gtcggcacaa tcatcgtacc gctgcttact ttgatgttca cgcccttcct gttcggctgg
     2881 gcgcgtccga ttcctatcga ttcgcgcaac ttccgcaacc cgcgccttgc ctggcgttgc
     2941 gttgccgcgt ccggcccgct gtcgaatcta gcgatggctg ttctgtgggg cgtggttttg
     3001 gtgctgactc cgtatgtcgg cggggcgtat cagatgccgt tggctcaaat ggcaaactac
     3061 ggtattctga tcaatgcgat tctgttcgcg ctcaacatca tccccatcct gccttgggac
     3121 ggcggcattt tcatcgacac cttcctgtcg gcgaaatatt cgcaagcgtt ccgcaaaatc
     3181 gaaccttatg ggacgtggat tatcctactg ctgatgctga ccggggtttt gggtgcgttt
     3241 attgcaccga ttgtgcggct ggtgattgcg tttgtgcaga tgttcgtctg actggctttc
     3301 agacggcata aacgctccag aaaacgcggc aggacatatt gccctgccgc gttttcctgt
     3361 agtgtaatct tatttttttc atcattatta gaaccaggtt gcatgataat acctttcatt
     3421 aactgaaaca ctgattaaga aactccagtc tgtctaatga tgaggttttc acatcgccaa
     3481 aacttgccaa tcaaatgctg gatttattgc cgtctgagat ttggtcaaat ccaaaggcga
     3541 cattcttaga ccctgtgtgt aaatcagggg tatttttgcg tgaaatcgtc aaacgcttgg
     3601 atgaaggctt gaccaatcaa ataccagata aacaaactcg cattaaccac attttaaaaa
     3661 atcaagtttt tggaagtact gccacgtatg taggtagctt tgaccgatat ttgcataaaa
     3721 actcctttgc tggtgaaagg aattattttg ccaattttaa aatatttctg gcaccaaata
     3781 gtacaatgac aaagacaatc atgccaatga ttaaatcagg atagctagaa tgagtcaata
     3841 acgtcaatgc tcccgccgct atcacaccga tattgatgat aatgtcattg gatgtaaaaa
     3901 tcatgctggc tttgatatgg atttctttat tttgattttt gctcagtaga tataagcaca
     3961 gccagtttgc aatcaatgcc aaaaatgccg tgccaatcat cagttgataa ttgggcagct
     4021 gctcagcacc gataaaacgc ctaatcactt ctatcacccc aaataacgcc aatattatct
     4081 gcgttatccc cgccaaaaat gccacacgtt ttttatacgc cagcgtcata ccaatggctg
     4141 atagcgccaa tatatagaca aagctgtccg ccagcatatc tagactatca gcaatcagcc
     4201 ccatagaatt agcaaaaata ccaaccgaac actctatgat aaaaaacaca aagttaatca
     4261 tgagcacttg atataataat cttttttcta agtgctcatc aggcttgtta aacactatct
     4321 tatcaacaat cacttcggtg gaaatgatat gactatcaaa attaagcggt tcaagtactt
     4381 gtaaaatcgt tgtatcttga ttatcgtgat agacggttaa gcaccgccca gcaatatcaa
     4441 actgtaattc ataaatatca gacacatctt ttaaacgcat gcgaatgagc tgttcttcgg
     4501 acgggcagtc cattttggta atgttaaaaa tggtcttttt catctattta gttccttgtt
     4561 ttgatcaggt tggctcaaat aaatctgtgt ttatattgct gcttggtaat ttttggatgg
     4621 tttgagtaaa ttgattaggt taaaatttac ctttggaagt accgccacgc ataatagttt
     4681 agatatgttt ataatctctg gataaaaaaa cgtaataagt gcttactgga taacaaagtc
     4741 caaaccaata gcaggcaaaa taaggcatcc accccccttc ttcattaagg atatatattg
     4801 agaaacaaat cgcaactaaa cagaaaaaac ttgggagata aagccatttc attcccctat
     4861 tcaagaatct agccaagata ggtattttgt attctacaaa aaagaaaggc atttccaagg
     4921 gaaacatgtc agataaaaac ttttgtttat tttttactat agatagaacc ttgcttctca
     4981 agagaaagcc attaataata ccgatgacag ctattaatat atagagaata gtataagtat
     5041 gaataatctt cattagacaa aaagaagaaa tggcagataa attacatacg atatattgga
     5101 atataaaata tttacggtct aaaccttgtt cagttgcaat ttttttaaaa ttgccttgca
     5161 taaaaaaatc aaaggcgtcc attaaactat ctttcacatt agaaatttaa aagctaaata
     5221 atacgacaaa caatgtgaag tactattcat ggtttattta aaaaataata ctattctgaa
     5281 cattatttag atacagaaat taacaaatta gaactaaaca agcttttaaa tactttaatt
     5341 ttattggaaa gctataaaag gaactataac tttacacact agtcacttct ttttaagagg
     5401 caaaagggat tgggaaggtc gtcttggaga taagcactgg tatttcggcc aatggtaaat
     5461 agagtttacc tcaaataggg tagaacctcc ttcatctgtc agttaataac agccactttt
     5521 acaatgccct gtcaaaataa agcggcacgc ccgatttttc actcatcgtc atcaaataac
     5581 ccatcacctt ttggggccat tcgatgccgc gcaccacggt cagattcctc aaaacgggga
     5641 aaaccaaaat atcctccata ccgattccgc cgttgatgcc gtctgaagca ccgtccatca
     5701 aattttccaa ctcttgcaaa tctgcgttta tccgttcgag gtattgggcg gttttattca
     5761 aattggcgga aaagctgccg atgcttttct cttttttgtc tgtaaaatat ttcaccgctt
     5821 ccggcgttgc aaattcaggc agcccgattt tgatcacgcg cggctgcacc agtttgtcgt
     5881 tgtatccgcc caccttgtcc agccacgccc gtatctcggg gcggacttcg tctttcagac
     5941 ggtcttcgcg gtcgaaatgc cgcacaatgt ccaaactctc gcccataaac gaaccgtctt
     6001 ctttttgcag gacgggcact tgtttcgcac cgatcatacc gatcggcgtt gcctcgtcgt
     6061 cgtttgccag cacggcttct tcaacgtccg cgccaaacag cccggcagcc atccgcgcac
     6121 gcacgcaaaa cgggcaatgg tcgtaaatat acagtttcat caaaatattc ctcgtcaacc
     6181 tgtcggtacc gactacctta acaccccgcg ccgcccgaaa caagtttatc ttcccgccta
     6241 tgcaccgtaa ataaataagc tgttacaata aactcgtttt tatcggaacg gaagacccca
     6301 tcatgaccgc catcagcccg attcaagaca cgcaaagcgc gactctgcaa gaattgcgcg
     6361 aatggttcga cagctactgc gccgctctgc cggacaacga taaaaacctc atcggtaccg
     6421 catggttgct ggcgcaggaa cattaccccg ccgatgccgc cacgccgtat ggcgagccgc
     6481 tgcccgacca cttcctcggc gcggcgcaaa tggttcatga actcgacctg ctccccgatg
     6541 ccgtcgccgc caccctgctt gccgacatcg gacgctacgt ccccgactgg aacctattgg
     6601 tttccgaacg ctgcaacagt accgtcgccg agctggtcaa aggtgtggac gaagtgcaga
     6661 aactcaccca cttcgcccgg gtggacagcc tcgccacgcc ggaagaacgc gcccagcagg
     6721 cagaaactat gcggaaaatg ctgctggcga tggttaccga catccgcgtc gtgttaatca
     6781 aactggcgat gcgtacgcgc accctgcaat ttttaagcaa cgcccccgac agccccgaaa
     6841 aacgcgccgt cgccaaagaa accctcgaca tcttcgcccc gctcgccaac cgtttgggcg
     6901 tgtggcagct caaatggcag ctcgaagatt tgggcttccg ccatcaaaag cccgaaaaat
     6961 accgcgaaat cgcgctgctt ttggacgaaa aacgcaccga acgcctcgaa tacatcgaaa
     7021 acttcctcaa catcctgcgc ggtgaactca agaaatacaa tgtccatttc gaagtcgccg
     7081 gccgcccgaa acacatctac tccatttaca aaaaaatggt gaagaaaaaa ctcagcttcg
     7141 acggcctctt tgacatccgc gccgtgcgaa ttctggttga taccgtcccc gagtgttaca
     7201 ccacgctggg tatcgtccac agcctctggc agcccattcc cggcgagttc gacgactaca
     7261 tcgccaatcc caaaggcaac ggctataaaa gtttgcacac cgtcatcgtc ggcccggaag
     7321 acaaaggcgt ggaagtacaa atccgcacct tcgatatgca ccaattcaac gaattcggtg
     7381 tcgccgccca ctggcgttac aaagagggcg gcaagggcga ttccgcctac gaacagaaaa
     7441 tcgcctggtt gcgccaactc ttggactggc gcgaaaacat ggcggaaagc ggcaaggaag
     7501 acctcgccgc cgccttcaaa accgagcttt tcaacgacac gatttatgtt ttgaccccgc
     7561 acggcaaagt cctctccctg cccacgggcg cgacccccat cgacttcgcc tacgccctgc
     7621 acagcagcat cggcgaccgt tgccgcggtg cgaaagtcga agggcagatt gtgccgctgt
     7681 ccaccccgct cgaaaacgga cagcgcgtcg aaatcattac cgccaaagaa gggcatcctt
     7741 ccgtcaactg gctttacgaa ggctgggtca aatccaacaa ggcaatcggc aaaatccgcg
     7801 cctacatccg ccagcaaaac gccgacaccg tgcgcgaaga aggccgcgtc caactcgaca
     7861 aacagcttgc caaactcacg cccaaaccca acctgcaaga gcttgccgaa aatctcggct
     7921 acaaaaagcc agaagacctc tacaccgccg tcggacaagg cgaaatttcc aaccgcgcca
     7981 tccaaaaagc ctgcggcacg ctgaacgaac cgccgcccgt acccgtcagc gaaaccacca
     8041 tcgtcaaaca gtccaaaatc aaaaaaggcg gcaaaaacgg cgtgctcatc gacggcgaag
     8101 acggtctgat gaccacgctt gccaaatgct gcaaacccgc gccgcccgac gatattatcg
     8161 gcttcgttac ccgcgagcgc ggcatttcag tgcaccgcaa aacctgcccg tctttccaac
     8221 acctcgccga acacgcgccc gaaaaagtgc tggacgcaag ctgggcggca ttgcaggaag
     8281 gacaagtatt cgccgtcgat atcgaaatcc gcgcccaaga ccgctccggg cttttgcgcg
     8341 acgtatccga cgcgctcgcc cgccacaaac tcaacgttac cgccgtgcaa acccagtccc
     8401 gcgacttgga agccagcatg aggttcacgc tcgaagtcaa acaagtcaac gacctcccgc
     8461 gcgtcctcgc cagcctcggc gacgtcaaag gcgtattgag cgttacccgg ctttaaatac
     8521 aaaaatgccg tctgaaagcc gaataacgct tcagacggca ttttgattgc cggggtttgc
     8581 tattttttgt tgcatagt
//
LOCUS       DAWGRZ010000080         8568 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000080 DAWGRZ010000000
VERSION     DAWGRZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8568
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.080"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(94..990)
                     /gene="argB"
                     /locus_tag="WHT44_001249"
     CDS             complement(94..990)
                     /gene="argB"
                     /locus_tag="WHT44_001249"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ2533431.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRLPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1160..2290
                     /locus_tag="WHT44_001250"
     CDS             1160..2290
                     /locus_tag="WHT44_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ2533432.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFIEGLLGRLLDPQDPTARALLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTVEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRIAALEAQFKNALLNASPDFQDEYGYEKDAPGEANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNVIGDLR"
     gene            2361..3212
                     /locus_tag="WHT44_001251"
     CDS             2361..3212
                     /locus_tag="WHT44_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ2533433.1"
                     /translation="MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIRLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFTVIWLFSKKQ
                     RSTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3295..3957)
                     /locus_tag="WHT44_001252"
     CDS             complement(3295..3957)
                     /locus_tag="WHT44_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ2533434.1"
                     /translation="MDSRKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEENGAIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            complement(4052..5845)
                     /locus_tag="WHT44_001253"
     CDS             complement(4052..5845)
                     /locus_tag="WHT44_001253"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ2533435.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAEAQGRPLDLNAFLQKNESLERTEMTQTNRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            6090..7199
                     /gene="proB"
                     /locus_tag="WHT44_001254"
     CDS             6090..7199
                     /gene="proB"
                     /locus_tag="WHT44_001254"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ2533436.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITCQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDALAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            7212..8474
                     /locus_tag="WHT44_001255"
     CDS             7212..8474
                     /locus_tag="WHT44_001255"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ2533437.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAATADI
                     LAANRQDLEAAAGNIPESMTDRLLLDGKRICAMADGIRAVAALPNPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGRESSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPIRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVW
                     HIETHSTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         1919 a         2430 c         2248 g         1971 t
ORIGIN      
        1 tcaggaatga cggtttggaa attgcccgaa accccaaaaa cagaaaccag acaaacaggt
       61 tttccgccaa agccggcatt ttccgacttt gcttcaggca tcttccccac cgcccaaaat
      121 catcgaaccg ataccggcat cggtaaagat ttccagcaaa agcgcgttgg gcaacctgcc
      181 gtcgatgata tgcgtggctt tcacaccgtt gacggcggct tcgaccgcag aagcgatttt
      241 cggcagcata ccgccataca gcgtgccgtc ggcaatcagt tcatcaatcc gtttcggcgt
      301 gagtttggtc agcagattgc ccgttttgtc catcacaccg gcgatattcg tcatcatcaa
      361 gagtttttcg gcgttcaatt cttccgccaa tttgcctgct accaaatcgg cgttgatgtt
      421 gaacgcttcg cctttttcac ctacgccgac gggggcgacg acgggaatgc agccacgttc
      481 tatcagccct ttaaccaaac cggtatcgat gctttccacc gtaccgacct gtccgatgtc
      541 cacgccattc tgttcgggcg tatcgatcaa aagtttcttc gccttaatga aatggtcgtc
      601 gcgtccgctt acgccgaccg cgtgtccgcc atatgtgtta atcatcgaca cgatttcttt
      661 attgacatgc ccgcccaaca ccatttcgac aatatccatc gcctctttgt cggtaacgcg
      721 cattccttgg acaaactcac cctttttgcc gactttttca agcatcgcat tgatctgcgg
      781 cccgccgccg tgaacgatga cgggatgaat gccgaccagc ttcagcagca cgacatcgcg
      841 ggcaaaccct tctttcaagg caggttcggt catcgcgttg ccgccgtatt tgatgacggc
      901 gaccgaaccg gaaaaccggc ggatgtaagg cagcgcttcg gcaaggatac gcgccttgtc
      961 ggcggcggaa ataatgtttt cagactccat aacggctccc tattgttgac aatttcggct
     1021 tatgttaacg ccaaacgccg tctgaaacaa tcttcagacg gcatccggca gactttcggg
     1081 caggttgaag ccttgcgata cgggcggtaa aatcgcggtt tatccggttc gagaaaccgg
     1141 cgacagcaag gactttccca tgattaaaat cagcacccgc ttcgatgccg gctcggtcgt
     1201 cgtcaaagac ctgaccgatc cttccaacat ccgcctcgcc ctgcgtcccg acaacgcctc
     1261 cgattttgca caatggttct acttccggct gcaaggcgcg gcatatcaaa actgcatcat
     1321 gcattttgaa aacgcggcag aagccgccta cccgaaaggc tgggaaggct atcaggcgtg
     1381 tgcctcatac gaccgccgca actggttccg cgtaccgact tcctacgaaa acggcgtact
     1441 gaccgtcaat catactccgc tgtccaacag cgtgtattac gcctatttcg aaccttactc
     1501 cgaagaacag catttaaacc tcctcggcga cgcgcaaggc agcggcctgt gccgcatcga
     1561 cgacttgggc agcaccgtgc aagggcgcga catcaatctg ctgaccatag gcaatcaggt
     1621 cgaaagcgat ttgaaaatct ggattaccgc ccgccagcat ccgggagaaa ccatggcgga
     1681 atggtttatc gaggggctgc tcggcagatt actcgacccg caagacccta ccgcccgcgc
     1741 cttgctcgac cgcgccacgt tctatatcgt ccccaatatg aaccccgacg gttcggcact
     1801 gggcaacctt cgtaccaacg ccgccggcgc gaacctcaac cgcgagtggg aaaacccgac
     1861 ggtagaaaaa agcccggaag tgttcttcgt gcgcgaaaaa atgctggaaa ccggagtgga
     1921 tttgtttttg gacatccacg gcgacgaagg gctgcccttt gtctttgtcg caggtacgga
     1981 aggcgtgccg aactacaatc cgcgcatcgc cgcgttggaa gcgcagttta aaaatgccct
     2041 tttaaacgcc agccccgatt tccaagacga atacggttac gaaaaagatg cgccgggcga
     2101 agcgaatatg accttggcga ccaactgggt cggcaaccgt ttcaactgcc ttgcctatac
     2161 gctggaaatg ccgttcaaag acaatgccaa tctgcccgac gacgacttcg gctggaacgg
     2221 tcagcgttct ttacggctgg gcgaagccgc gttgtccgct atcctcaacg tcatcggcga
     2281 tttgcgctga aacccttatt gcagaaatgc cgtctgaagc atcatgcctt tcagacggca
     2341 tttgaaaacg aaagcccgtt atgattaccc atccccaatt cgatcccgtc cttatcagta
     2401 tcggcccgct tgccgtccgc tggtatgccc taagctacat cctcggattt attcttttta
     2461 cctttctcgg cagaaggcgc atcgcgcaag gcttgtccgt ttttaccaaa gaatcgctcg
     2521 acgacttcct gacatggggc attttgggcg taattttggg cgggcgtttg ggttacgtcc
     2581 tgttttacaa gttttccgac tacctcgccc atccgcttga tattttcaag gtatgggaag
     2641 gcggaatgtc gttccacggc ggctttttgg gtgtagttat tgccatacgg ttgttcggcc
     2701 gcaaacacgg catcggcttc ctcaaactga tggatacggt cgcaccgctc gttccgctgg
     2761 gtctcgcttc gggacgtatc ggcaacttca tcaacggcga actttgggga cgcgttaccg
     2821 acatcaacgc attttgggca atgggcttcc cgcaggcgcg ttacgaagat gccgaagccg
     2881 ccgcgcacaa tccgctttgg gcagaatggc tgcaacaata cggtatgctg ccgcgtcatc
     2941 cctcgcagct ttatcagttt gcacttgaag gcatctgcct gttcaccgtc atttggctgt
     3001 tctctaaaaa acagcggtcg accggacaag tcgcctcgct cttcctcggc ggctacggca
     3061 tattccgctt cattgccgaa ttcgcacgcc aacccgacga ctatctcggg ctgctgacct
     3121 tggggctgtc gatggggcaa tggttgagcg tcccgatgat tgttttgggt atcgtcggct
     3181 ttgtccggtt cggcatgaaa aaacagcact gaccgcctgt gaaaaatacc gtctgaaaca
     3241 tggaaatccg gtttcagacg gtatttttac gtttggttca tggaaacaaa cgcttcaatt
     3301 cttaccgcaa cacgccttgt attttctgcc gctgccgcac ggacagggat cgttcctgcc
     3361 tgttttttcg ccttccctgc ggacggtttg cggtttgttg atgactgcct gccagtagcg
     3421 gtagatatcc gccaatgcat aaggcaactc ggattccagt tccgccagct cgccttctgt
     3481 gaattgcaga cggatagcgc cgttttcctc ttcgtcgtaa ataccgccca atgccatgat
     3541 gggataaaac aactcttcaa acgcttcatc atcgacggct tcaaaccaat cggtcggcac
     3601 aatatccaaa ccgtaaagat aagcattgca ccatgtgtaa aaatcgctgc cgccgtcttc
     3661 gttttcatac agccacaaat cgggcagttt tttatccgac atcgcggcgg ttgtttccat
     3721 cgccattgcc aaaaccagcc gttcgatttc ggaacgttcg gcggcggtaa attgcgattc
     3781 gtcgcccaac acttcgggca gccagtcgag cggtgtcaat ttgtccggcc cgctcaacag
     3841 cgccgtcata aaaccttgaa cctcgtcgca acgcatcgtg ttgccttgtt cgcttttggc
     3901 atccaacaat tcgctcaacc gccgtttgga tgcttcggta aattttcggg aatccatcat
     3961 tttccttttc aaatgggttt tgcgccctat tatcgccgca atgccgtctg aagccttttt
     4021 atgcttcaga cggcatcgtc tgttttcacg ctcaaatcgt accgctgccc tgcgctttcg
     4081 gtttggcggc actaaattcc agcttgctca aagcggaaag gtaggctttg gcggtggcga
     4141 ccaaaacgtc ggtatccgcg ccctgaccgt tgacgacgcg gttgccgcgc gccagacgga
     4201 cgctggtttc gccctggctt tccgtacctt gcgtgacggc gttgacggaa taaatctgca
     4261 aagccgcgcc gctttgcgcc acgctttcaa tcgctttgaa aatcgcatcc acggggccgg
     4321 aaccggttgc ggaagcgcgt ttttcttcac ctttgatgct gaacacgatg tcggcgcgcg
     4381 gttcttctcc ggtttcggtg ctgatttttt gggagatgaa tttgtagctc tcggcattca
     4441 tgctgcccat ttcgtcggat accagtgcgt gcaggtcttc atcgaagatt tcgcgttttt
     4501 tgtcggcgag ttctttgaag cgtgcaaatg ccgcgttcag tgcctcttcg ctttccaact
     4561 cgatgcccaa atccgccagc ttggttttga aggcgttgcg gccggacaat ttgcccaagc
     4621 tcaaacggtt tgttgcccag ccgaccgatt cggcggacat aatctcgtaa gtttcgcggt
     4681 gtttcagcac cccgtcctga tggatgcccg attcatgcga aaaggcattg gcaccgacaa
     4741 tggctttgtt gggctgcacg ggatagcccg taatggtgga caccagtttg gacgaaggca
     4801 cgatttgcgt ggtatcgatg ccggtttcca agccgaacaa gtcgtggcgc actttcaacg
     4861 ccatcacgat ttcttcaacc gaagcattgc ctgcacgttc gcccaagccg ttgacagtac
     4921 attccacctg acgcgcgccg ccttttaatg cggcaagcga attggcaacc gccaagccca
     4981 aatcgttgtg gcagtgtgcc gaccaaacga ctttgccgcc gttgggcgtt ttggcaatca
     5041 gttcgcggaa aaattcttcg gttttatacg ggatggaata gccgacggta tcgggaatat
     5101 tgatggtggt cgcgcccgct tcaatcaccg cgccgcagat ttcggcaagg aaatcgattt
     5161 ccgaacgcaa cgcgtcttcg caggaaaatt ccacatcgtc ggtgtattca cgagcgattt
     5221 tcaccgcttt gaccgccgcc tcaatcacct gcttcggctt catcttcaat ttgtactcca
     5281 tatggatggg gctggtggcg atgaaggtgt ggatgcgttt tttcggcgcg ggcgcaacgg
     5341 cctcacccgc ctgacggatg tcccgctcga tggcgcggga caatgaacag accgttgatt
     5401 tggtaatggt tttcgcaatc gcattgaccg cctcgaaatc gcccgggctg gcagcggcaa
     5461 aacccgcttc gatgatgtcc acacccaatt tttccagctg gcgggcgacg cggatttttt
     5521 cctctttggt catagcggcg ccgggcgatt gttcgccgtc gcgcagggtg gtgtcgaaaa
     5581 tgataacgcg gttggtctgt gtcatttctg ttctctccag agattcgttt ttttgtaaaa
     5641 aagcattcaa atccagcggc ctgccttgcg cttccgccaa cgcggtcagc ttttgcagtt
     5701 gcgccagagg cagcgagccg cgcgtgcgcc atttatagat ggcggcggtc gtcgcggcat
     5761 tttcgggatc gtgctgtttc aacgcttcgg caagcgcgtt cacgccgccg aaataagcaa
     5821 ccaagcggtc tatatctaat tgcatggtag tccttgtcta aaataaatca gcctacagca
     5881 gaaatatgga caggattata cgcattttgg acaaaacggc aatataaatt tgaaaaatct
     5941 caataagccg ccatagattt tattaatttt atacattttg tccattttat tgcagacgcg
     6001 gcgtgattgt tgacaattgc ggcagaacag ggcaaattcg gcggcacaag gtggcaacga
     6061 gacaggacaa accaacaagg acggaaacaa tgaaatacaa aagaatcgta tttaaagtcg
     6121 gcacatcttc gattacccat tcggacggca gtctctcgcg cggcaaaatc caaaccatca
     6181 cctgccagct tgccgcattg catcatgcgg gacacgagct ggtcttggtg tcttccggcg
     6241 cggttgcggc agggttcggt gcgctgggtt tcaaaaaacg tccggtcaaa atcgccgaca
     6301 aacaggcttc cgccgccgtc gggcaggggc tgctgatgga agaatatacg gcaaacctgt
     6361 cttcagacgg catcgtgtcc gcgcaaatcc tgctcagccg cgccgacttt gccgacaaac
     6421 gccgctacca aaatgccggc ggcgcacttt ccgtgctgct gcaacgccgc gccgtcccca
     6481 tcatcaatga aaacgatacg gtttcggttg aggaattgaa aatcggcgac aacgacacat
     6541 tgagtgcgca agtggcggcg atgatacagg cagacctctt ggtgctgctg accgacatag
     6601 acggtcttta cacgggcaac ccgaacagca atcccgatgc cgtacggctg gacaaaatcg
     6661 aacacatcaa ccatgaaatc atcgaaatgg cgggcggctc gggttcggca aacggcacgg
     6721 gcggtatgct gaccaaaatc aaagcggcaa ccatcgccgc cgaatccggc gtaccggtgt
     6781 atatctgttc ctcgctcaaa cccgatgcac ttgccgaagc tgccgaacat caggcggacg
     6841 gctcgttttt cgtcccccgt gccaaaggtt tgcggacgca gaagcaatgg ctggcgttct
     6901 attccgaaag ccggggcagc gtttatgtgg acgaaggtgc ggaacacgct ttgtccgaac
     6961 aggggaaaag cctgctgatg tcgggcattg ccggaatcga agggcatttt tcccgtatgg
     7021 acaccgtaac cgtgtacagc aaggcaacca aacagcccct gggcaaaggg cgcgtcctgt
     7081 tcggctctgc cgccgccgaa gacctgctca aatcgcgtaa ggcgaaaggc gtgttcatcc
     7141 atcgggacga ctggatttcc atcacgcccg aaatacgcct gcttctgacc gaattttaga
     7201 aaaggaaacc catgtcaaac acacaaaaac agcttgccct tgccaaagcg gcaaaaaaat
     7261 ccgtcaacac ggcggatacg gaagaaaaaa accgcgcgct gcttgcgatg gcggacagct
     7321 tggaagcggc gacggcagat attttggcgg caaaccgtca agacttggaa gccgcggcag
     7381 gcaacattcc cgaaagcatg accgaccgcc ttttgttgga cggcaaacgc atttgcgcga
     7441 tggcggacgg catcagggcg gttgccgcgc tgcccaaccc cgtgggcgaa atactggaaa
     7501 cctcgacttt gcccaacggc ttggaaatcg tcaaaaagcg cgtggcgatg ggcgttatcg
     7561 gcattattta cgaaagccgc ccgaacgtta cttccgatgc ggcggctttg gcactgaaaa
     7621 gcggcagcgc ggtcgtactc cgcagcggca aagatgcatt ccaatccgca cgcgccatcg
     7681 ttgccgccct gaaaacgggg ttggcgcaaa cccgcatcga ccccgacgcg ctccaactga
     7741 ttgaagacac cgggcgcgaa agcagttacg aaatgatgag ggcgaaagat tatctagacc
     7801 tgctgattcc gcgcggcggg gcggggctga tacgggcggt ggttgaaaat gccgtcgtgc
     7861 cggtcattga aacgggaacg ggcattgtcc acatttatat cgacaaggac gcggattggg
     7921 acaaggcgct ccgtatcgtg tacaacgcca aaaccagccg tccgtccgtg tgcaactcga
     7981 tggaagtgct gctggtacat gaagacattg ctgccgactt cctgcccaag ctcgaacggc
     8041 tgttggttcg cgaccgcata gaagccggac tgccgcccat ccgcttccgt ttggatccgc
     8101 aggcggcgcg gcatatcggc ggcgaagcgg cgggtgcaga cgatttcgat accgagtttt
     8161 tagactacat cctcgctgtg aaaaccgtcg cttcggtcga agaggcggtc tggcacatcg
     8221 aaacccacag cacacaccat tcagacggca tcgttaccga aaaccgccac gctgcggact
     8281 atttcacgac tcatatcgat tctgccgccg tgtatgtcaa cgcgtccacg cgctttaccg
     8341 acggcggcga attcggcttg ggttgcgaaa tgggcatctc cacgcaaaaa ctccacgccc
     8401 ggggcccgat gggtttgaaa gagctgacga gttacaaata catcgtacaa ggcacgggac
     8461 aggtcaggga ataatccgaa ttcaaatgtc tgatatagtg gattaacttt aaaccggtac
     8521 ggcgttgcct cgccttagct caaagagaac gattctctaa ggtgctga
//
LOCUS       DAWGRZ010000081         8567 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000081 DAWGRZ010000000
VERSION     DAWGRZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8567
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.081"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(242..3094)
                     /gene="gcvP"
                     /locus_tag="WHT44_001256"
     CDS             complement(242..3094)
                     /gene="gcvP"
                     /locus_tag="WHT44_001256"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ2533438.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPDALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSGRFFVDERVYPQTLDVMKTRAKYFGFELVVGDFVKADEGEYFGALFQYVGKDG
                     DVQDLQDVISRLKAKGTIVVVATDIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDAFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGIKVVHEVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSAYEDLVDLYRAFTGKDAFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLADMENSLKSITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQYRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHALTDTHSTSAGQT
                     AVAAAAYGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKALGEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVGRGEWPKDDNPLVNAPHTAADITGNWAHPYSREEAVFPL
                     PFVREHKFWPSVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3125..3484)
                     /locus_tag="WHT44_001257"
     CDS             complement(3125..3484)
                     /locus_tag="WHT44_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533439.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNIVFPLPAYPLAWLVPAGFMVLGGGFPLSLPLVALLVLTCCILAHCPPLS
                     RLLCYPCPNHPMSKNSA"
     gene            3432..3662
                     /locus_tag="WHT44_001258"
     CDS             3432..3662
                     /locus_tag="WHT44_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ2533440.1"
                     /translation="MISACPGFIHSRLGRSVMPSEPAGRLFQKTPYRCNFMRLYGVGF
                     ALQTAYASRSSLVSQSVHDYINNLGADKPLFI"
     gene            complement(3655..4296)
                     /locus_tag="WHT44_001259"
     CDS             complement(3655..4296)
                     /locus_tag="WHT44_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ2533441.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPEEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4286..4837)
                     /locus_tag="WHT44_001260"
     CDS             complement(4286..4837)
                     /locus_tag="WHT44_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ2533442.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFDEADIDRLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDTHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRKTLKYHPKWLDAV"
     gene            complement(4841..5359)
                     /locus_tag="WHT44_001261"
     CDS             complement(4841..5359)
                     /locus_tag="WHT44_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ2533443.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5359..6999)
                     /gene="pqiB"
                     /locus_tag="WHT44_001262"
     CDS             complement(5359..6999)
                     /gene="pqiB"
                     /locus_tag="WHT44_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ2533444.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATQGGGLDDLQVKLADLLDKFGKLPLDKTVSGLN
                     ASLAELKSTLKSINTLSNNPQTQNIPNELNQTLKELRTTLQGVSPQSPIYGDVQNTLQ
                     SLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(6992..8452)
                     /locus_tag="WHT44_001263"
     CDS             complement(6992..8452)
                     /locus_tag="WHT44_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ2533445.1"
                     /translation="MGSAVGGQPLEKIFLPDTKGLFILPNLPVLHPDWVYRLFSFIMP
                     PLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRADVPQLDRGEAAFCPR
                     CGYKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPGAASVLSLPEMMRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGDN
                     AVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLSISSAFLTAAVILYFPA
                     NILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIA
                     SARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKHD"
BASE COUNT         1913 a         2205 c         2419 g         2030 t
ORIGIN      
        1 ttgtttgtcc gtgtggaaat gacatcaaag ggtgtgaata ggcttttcat ctttgaaatc
       61 tgtctgcccg atcggatgaa taaaaagtag gatgataaaa aagctgatgt atggatactg
      121 gtttaaccca tttgttttaa agttatagtc gagatacgtg tatcggtgca accagtctgc
      181 cgtcgacgaa aatgccgtct gaaagtgttt cagacggcat ttgtcttcgg ggtttcaacg
      241 gtcagtcttc ataattttcc atcggtgggc agctgcacac gagattgcgg tcgccgtaca
      301 cgtcgtccac acggttgacg ctcggccaga atttgtgttc gcggacgaac ggcaatggga
      361 agacggcttc ttcgcgggaa tacggatgcg cccagttgcc ggttatatct gcggcggtgt
      421 gcggcgcgtt gaccagtggg ttgtcgtctt tcggccattc gccgcgcccg actttcagca
      481 cttcctgttt gatttgtttc agggcggcga tgaagcggtc gagttcggct ttgctttcgc
      541 tctcggtcgg ctcgatcatc agcgtaccgg caacggggaa ggaaaccgtc ggggcgtgga
      601 agccgtagtc catcaggcgt ttggcgatgt cggtttcggt aatgccgctt tcggctttga
      661 gcggacgcaa gtcgacgata cattcgtgcg cgacgcggcc gtttttgccg gtgtagagaa
      721 tcggataatc ctcgcccaag gctttggcga cgtagttggc gttgagcaat gcccagcgcg
      781 ttgcctgttc catgccttgt ttgcccatca tggtcaggta catccaagta atcggcagga
      841 tggatgcaga accatatgct gcggcagcca cggcggtttg cccggcactt gtgctgtgcg
      901 tgtcggtcaa agcgtgtccc ggtgcaaacg gggcaaggtg ggctttcaag ccaatcggac
      961 ccatgcccgg gccgccgccg ccgtgaggga tacagaaggt tttgtgcagg ttcatgtgca
     1021 acacgtccgc gccgacttcg gcaggctgca tgatgccgat ttgagcgttg aggttggcgc
     1081 cgtccatata aacctgtccg ccgttttcat ggatgatgcg gcagatgtcg cggatgcctt
     1141 cttcatacac gccgtgggtg gacggatagg taatcatgat ggcggacaaa gcgtcgcggt
     1201 attgctcggc tttggctttc aaatcgtcga tgttgacgtt gccgtgttcg tcggtatcga
     1261 cgacgacgac tttcaagccc agcatggccg cagtcgcagg gttggtaccg tgggcggatt
     1321 tgggaatcag gcagatgttg cggtgcgctt cgccttgggc ttcctgatag cggcggatgg
     1381 caagcatacc gctgtattcg ccctgcgcgc cggagttggg ctggaaggaa attgcatcaa
     1441 aaccggtgat ggatttcagg ctgttttcca tatcggcgag caattcgcgg tagccggcgg
     1501 tttgcgcttc aggagcgtaa ggatggatgt cggagaactc cgcccaagta atcggcaaca
     1561 tttccgcagt cgcgttgagt ttcatggtgc agctgcccaa tgaaatcatg ctgcggttca
     1621 tcgccaaatc gcggtcttcg agttttttca gatagcgcaa catttcgtgt tcggtgtggt
     1681 aacggttgaa cacaggatgt tgcagaatgt catcttgacg cagcaattcg gcgttcagac
     1741 ggcctttgac atcatcggca aatgcgaacg catccttgcc ggtaaacgcg cggtacaaat
     1801 caaccaaatc ttcgtatgcc gatgtttcat ggaatgcagc cgcaacttga gtatcgttga
     1861 cgcggcgcag gttgtaaccc gactctaaag cagcggcaaa cacttggtct gctttctctt
     1921 tgttgccgaa atcgacggta acggtatcga agaagacttc gtgaaccact ttgatgccgt
     1981 ctgaaaccag tgcatcggca aaggcagaag ccagcgcgtg aatgcggttg gcaatgcgtt
     2041 tcacaccttc aggaccgtgg taaacggcat acataccggc caagttcgcc agcaatgcct
     2101 gcgcggtaca aatattggat gtcgcttttt cgcggcggat gtgttgctcg cgggtggaca
     2161 acgccatgcg caaggcaggt ttgcccgatg cgtctttgga tacaccgata atacggcccg
     2221 gggcggaacg tttgaacgcg tctttaaacg cgaaataagc ggcatgcggc ccgccgaagc
     2281 ccatcggtac gccgaagcgt tgggtgttgc ccaacgcaat gtccgcgccc aattcagccg
     2341 gagacttcag caaaaccaaa ctcatgatgt cggtggcaac gaccacgatc gtgccttttg
     2401 ctttcagacg gctgataaca tcctgcaaat cttgtacgtc gccgtctttg ccgacgtatt
     2461 ggaacagcgc gccgaagtat tcgccttcat ccgctttgac aaaatcgccg accaccagtt
     2521 caaagccgaa atatttggcg cgggttttca tcacgtccaa agtttgcgga tacacgcgct
     2581 cgtccacaaa gaaacgcccg gatttcacct tgcccacgcg gtgcgccatc gccatcgctt
     2641 cggcggcggc ggtcgcttcg tccagcaaag acgcgcccgc cacagggaaa ccggtcaaat
     2701 cgatacacac ttgttggaag ttcaacaaag cttccaaacg accctgggca atttccgcct
     2761 gatacggcgt gtaggcggta taccagcccg gattttccaa tacgttgcgc aaaatcacat
     2821 tcggcacgcg ggtcgggtaa tagcctaaac cgatatagga cttgttgatc acgtttttcg
     2881 acgcaatgcc tttcaatttt gccaacgcgt ccgcttcggt cagggcatcg ggcaaatcga
     2941 gttcggacgg catacggatg ctttgcggca cggtattgcc gacaaaatcg tccatacttt
     3001 tctcgccgac agcggcaagc aacgccgctt cgtcgccgaa actcaaatgc cgcgcggcaa
     3061 attcgttggg gttgaacagt tcggacagtt tcatttctac tccttcgggt ttgtagggct
     3121 gccatcaggc agaattttta gacatcggat gattcgggca agggtagcac aaaagacggg
     3181 ataatggcgg acaatgcgcc agaatgcagc aggtcaggac aagcagcgcc acaagcggca
     3241 gcgataaggg aaagccgccg cccaaaacca tgaaacctgc aggcaccagc caagccaatg
     3301 gatacgccgg aagcggaaaa acaatattga gcagaaccaa aatcacgaca aacagcggca
     3361 gacccgccat caggttggca cgcctgtatg ttgcgtacgg gtcggatgct tcttcaggtt
     3421 cgcaatattc catgatttca gcctgtccgg gctttatcca cagcaggctc ggacggtcgg
     3481 tcatgccgtc tgaaccggca ggccgtcttt ttcaaaaaac gccataccgc tgcaacttca
     3541 tgcggctgta tggcgtaggc tttgcacttc agacggcata tgcgtcccga agcagtttgg
     3601 tgtcccagtc ggttcatgat tatataaaca acttgggtgc ggataaacct ttgtttatct
     3661 aaacccctgt tttctcaaaa attgattcaa atcttcggca aatttccgat agcctttgcc
     3721 gttggcgtgg atttggtcgg atttcagatt attatcgccc aaaatttccg cccacgcgcc
     3781 gccgaacagc ggaatgccgt attcctcgga caaatcctca tacagcggat gatcgctcaa
     3841 atgcccgaac aacgcgccca aggtaatgtg cggcacgccg acgaggacgg cggggatgtt
     3901 ttccttctgc acggtttcga tgattttcgc gatattggcg cgggtctgct cctcgggaac
     3961 tttgcgcaga aagtcgttgc cgcctatgcc gacaatcaca agcttgggtt tgcgtgccaa
     4021 cagcgcgggc aggcgcgaca gggcttgggc ggatgtatcg cccgatacgc cgccgttgac
     4081 aatattccaa cccgtcagtt tttgcagttg cgcggggtag gattcgccgg ggtttgcgcc
     4141 gtagccgaag gtaagcgaat cgcccaaggc aagtacggtg cttccttcgg gaattttggc
     4201 gtgggttcgg gcggattttc tgccgcaggc ggtaaggagc aacgcgcctg cgccgaggag
     4261 gaaggttctt ctgttcattg gtttttcaga cggcatcgag ccatttcggg tggtatttca
     4321 gggttttacg gtaaaccgga cagctttcgg cgtgcgcgcc tttgaggtag ccggtactca
     4381 tcagaaattc gccgacgatt tcgccgccga caaatttgaa atgtttttta aagagtttga
     4441 cccattcgtc tttgcttcgc ggatggtgcg tgtcgagcca gttcttgaac gagccgtatt
     4501 cttgttgcaa cgcttggatt tgccgtgcat tgaaaatggc ggcatcgatt ttcaggcggt
     4561 tgcggacaat gcccgcatcg gcaagtaacc gatcaatatc ggcttcgtca aatgcggcga
     4621 ccgtatcgat gtcgaaccct tcaaatgctg tctgaaacgc ctgccgcttc ttcagcatca
     4681 gcgtccagct caatcctgcc tgattgattt ccaacaccag ccgttcaaac aattcattgt
     4741 cgtcctcaat cggaaaaccg tattgcgtgt cgtggtaatg tttgttcggg ttgtcggtgt
     4801 tttcgggaag tgaggcggca aattcgcaat agttcacggt ttactcgacc atctgttgcg
     4861 ccgcctgttt cagtccctgt tcgagtgcgg cggtcatcgc ggcgtagccg tcaccctgct
     4921 gttcggtttc gatatggaag ggtctgttcg taccgtcggg taggacggcg tagccgctga
     4981 tgagggtttt gcccgtgtag ctgccttgga atgcgtcaat atagaccgtc catttttcgg
     5041 tactgccgct gcgtgaggca ggaacaaaga tgcgtgtgct gtccaaacgg ttgaatgcat
     5101 tgctcaacgc cgcttcgagc atatcgtcca aggtgtccgc ccaaacgtgg ttttgtgcgg
     5161 tgttgaggcg gtaggggtcg gtttgataga ccagtccgcc gcgtttgagc ggttcggcaa
     5221 ggcggacttc gacggcggtt tcgccgcctt gcgttgcagg gcggatgtag cggctgtcgg
     5281 gcaacacgaa atattgtgtg ctttgcacag taccgcaggc ggcaagcgtc agggcggcgg
     5341 cgatggggaa aaggcgcatt atcggcttcc tttcgggata gggtctttgc tgctgctgtt
     5401 gaaaatcagc gcgttgggtt tttctttcaa agtgttaatg acgggttgaa cgtcttttaa
     5461 agttttgtcc aaactttgca gcgtattttg tacgtcgccg tagataggcg attgcggcga
     5521 tacgccttgc agggttgtgc gcaactcttt cagggtttgg ttcagctcgt tcggaatgtt
     5581 ttgcgtttgc ggattattgc ttagcgtgtt aatggatttc agcgtggatt tgagttcggc
     5641 gagcgaagcg tttaaacctg aaacggtttt gtctaaaggc agtttgccga atttgtccag
     5701 caaatccgcc aatttgacct gtaagtcgtc caaaccgccg ccctgggtcg cgataacggt
     5761 atcgcctgca taaacggtat gcggtcgcag cttgggcgaa gacgaaggct gatcgttcaa
     5821 ctcaatcatt ttgctgccgg tcagcaggtt gttgctggag atggtggcgg tcaggccttt
     5881 gtttaaagcc gtctgaaatt gttgtttcca atgctctttg ctttgttcgt cggcattgat
     5941 ttccaaacgg gaaggctcga tgcggatgcg tacgggtatc cagccgtttt caaataaatg
     6001 caggctgtcg ttgcggtcga aataagggac atcggaaacc acgccgacat tcagcccttt
     6061 gtactcgacg ggcgaaccga cggtcaggcc gcgcacggat tgtttgaaaa acgcggtgta
     6121 gtacagcgag cggtcgtcag gcaggttggc gacttcgctg cggctgtcgt aaagcgtgaa
     6181 gctgtcttcg cttttgacgt ttttactgtt tttggttttc ggcgaatcaa atgaaatcgc
     6241 gcccgacagc agggcaggca gaggggcgga attgagtttg atgccgctgc ctgtggtttc
     6301 gatattgatg ccgctttcca gccaaaaacg gctggcggaa tgaatcagtt tgtcgttggg
     6361 gctttggatg aagatggtgt aatgcacgct ttggtcggac gggtcgaaat gcgcgctttc
     6421 gacttgcccg accataaagt tctcatacaa aacagggctg ttgacgttga ggatgcggtc
     6481 gtttttacca atcaaattca agcgcagccc gctttgcccg atggcggtaa cgggcggaat
     6541 gtcctgcacc tggaacacgt cttttgcctc gtcgcttttg ccgggtgtaa aggcgatgta
     6601 cgaacccgaa agcagcgtac ccaaaccggt tacgccgctt tggtcgatac gcggcttgac
     6661 cacccaaaac tgtgtgtcgc tgcggatgag gccggatacg tccgcattga gttgggcggt
     6721 tacttccacg cctttttggt cgtcgcgcag tttgattcgg gtaacgcgtc cgacatcgat
     6781 gctcaatact ttgatgaccg tattgttgac ctcaatgcct tccgcgctgt ccatcaagag
     6841 cgtaaccaca ggccccctgt tgcggatttc cttaacccaa agccagccgc cggcaatcag
     6901 cgcgatcagc ggaaccagcc agacggcaga gaggaaggtg ttgtttttgc ggacgcgtgc
     6961 ttgggcgtgt ccgtttggag gagggctgtt gtcagtcatg tttttccgtt tcattgaaag
     7021 caatgccgtc tgaagcgcgt ttgtcccaaa gcaggcgcgg gtcgaaataa taggcggaca
     7081 gcatcgtcag aatcacgacc aggcagaaat agactgccgc actgcccgga atgacgcgcg
     7141 cggcataagt gtggaacgaa cacatcaaaa taataatcac aaaaatatca atcatcgacc
     7201 agcggccgac cgcttcggtg atgcggtaga ggtgcgacaa tttctttgca cccgttggca
     7261 aagcgaagcg ggcggacgca atcaaaaccg acattgccgc aatcttcagt accggcacca
     7321 aaatactcgc gctgaaaata accgccgcaa tcagcctgtc gccctcgtcc cacatataag
     7381 cgatgccgtt aaggatggta ttgacctccg tggcggcagg attggacgaa atcataatcg
     7441 gcaggatatt ggcagggaaa tacaaaataa ccgccgccgt cagaaacgcc gacgaaatac
     7501 tcagactttt cggccgtcgg cggtacagtt ccgcaccgca cacgccgcag ggggattcgg
     7561 cactgtcgcg gaaatacagg cagcggctgc aacaggtttt accttccgat gccgtctgaa
     7621 ccgcattatc ccccgtcagc cgcccgattt gaaaatacac ccaatgctgg ggaaccgata
     7681 ccgaagtccg aatcagcata accgacagcg cgaacatcag ataaaacgcc gatccgaagc
     7741 gaacctctgc cacagacgag agcttgatat acgccaccaa agtggaaaca aaaaacacat
     7801 ccaccatcat cgcctgtctc aagcgcacca tcacacgcgt tgccaaacgc agcgcaggat
     7861 acgcctgttt ccgtatcagc gcggcataga catacaggca cagcagcaga aacagaaccg
     7921 gcgcgccgaa ggtcagcaca aacatcactt cggccaaaaa accataatcc tgaaacacca
     7981 tcaggcgcat catctcgggc agcgaaagga cggatgccgc acccggtatc ccgacctcga
     8041 tatacgtcat accgtaagca aacgccatca aaatcagcga agccgccgca taagcgggcg
     8101 gggcggaaaa aggatgcctg cccaccctga agagcttata gccgcagcgc gggcagaacg
     8161 ccgcctcccc cctgtccaat tgcggcacat ccgcgcggca gccgcattcc gggcaatcta
     8221 ccgtatgcgg cggcagggct tcgtcgcggc agttgccgcg aaggctgtgg gggatatttt
     8281 cggcaaacgg cttcatcggg aaagtggcaa atataagtgg cggcattata aaggaaaaaa
     8341 ggcgatacac ccaatcagga tgcaaaaccg gcaggttcgg cagtatgaac aaacccttgg
     8401 tgtccggcaa aaatattttt tcgagcggct gtccgccgac tgccgaaccc atttgataaa
     8461 atcccgattc tgtaaagaat agtaaaatac ctgcttaaaa acatcacgca aatgatattt
     8521 tttccaaaaa tatgtgataa taaatttcgt ttatgattct ttattat
//
LOCUS       DAWGRZ010000082         8431 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000082 DAWGRZ010000000
VERSION     DAWGRZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8431
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.082"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            31..168
                     /locus_tag="WHT44_001264"
     CDS             31..168
                     /locus_tag="WHT44_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-like fold family protein"
                     /protein_id="HEZ2533446.1"
                     /translation="MHFHKANEFLGMTRLPTFLCNDVVKNPQVEKYLADYQAHLEKVF
                     G"
     gene            complement(376..1761)
                     /gene="pntB"
                     /locus_tag="WHT44_001265"
     CDS             complement(376..1761)
                     /gene="pntB"
                     /locus_tag="WHT44_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ2533447.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIAPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TLSSGSQEIGEYREVKAADIAEMLKGANNVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1841..2224)
                     /locus_tag="WHT44_001266"
     CDS             complement(1841..2224)
                     /locus_tag="WHT44_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ2533448.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRGFLAHTQ
                     GAAARAHAAQQNGFEAFAPFAAAVLTAHATGNAAQSTINTLACLFILFRLAFIWCYIA
                     DKAAMRSLMWAGGFACTVGLFVAAA"
     gene            complement(2228..3769)
                     /locus_tag="WHT44_001267"
     CDS             complement(2228..3769)
                     /locus_tag="WHT44_001267"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ2533449.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWVCPLIYKVNAPSEQELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAVPAA
                     KPEPKPVPLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLN
                     MFKKG"
     gene            4059..4892
                     /gene="serB"
                     /locus_tag="WHT44_001268"
     CDS             4059..4892
                     /gene="serB"
                     /locus_tag="WHT44_001268"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ2533450.1"
                     /translation="MPHALVLQFPSAAALPSDFPLRLPEPDCADEKRMRFIVEEGFSL
                     SEKDAALLGSRQIDHAVLPDMDFDELGLIVSDMDSTLITIECVDEIAAGVGLKNKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDERVLADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAVGDGANDIPMLKEAGIGVAYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            4956..6179
                     /locus_tag="WHT44_001269"
     CDS             4956..6179
                     /locus_tag="WHT44_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ2533451.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGADGVY
                     TSFREGVAQASGMRRVAVLTLCGAVAGSGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGKRLGLDEGEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLTV
                     NTSLLWFSAVIGPILGVAAVFFQRTAQKFPFIKRDNIKIIPLAVCMFALIGVISVWFP
                     EILGNGKAGNQLTFGGLTDWQHSLGLTAVKWLVVLMALAVGAYGGLITPSMMLGSTIA
                     FAAATAWNSVFPEMSSESAAIVGAAVFLGVSLKMPLTAIAFILELTYAPVALLMPLCT
                     GMAGAVWVAKKMGFK"
     gene            6343..7923
                     /gene="purH"
                     /locus_tag="WHT44_001270"
     CDS             6343..7923
                     /gene="purH"
                     /locus_tag="WHT44_001270"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ2533452.1"
                     /translation="MSSIKRALISLSDKTGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGTPEIAGFPGRFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLAAYKQLQGKELSYNNIADADAAWEAVKSF
                     DVPACVIVKHANPCGVAIASNTLDAYKLAYATDTTSAFGGIIAFNREVDGATVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTGIRHFRH"
BASE COUNT         1905 a         2284 c         2347 g         1895 t
ORIGIN      
        1 tttgaaggca aaggcgttga tgttttgtat atgcacttcc acaaagccaa cgagtttttg
       61 ggtatgaccc gcctgccgac attcttatgt aacgatgtgg ttaaaaatcc gcaagtggaa
      121 aaatacttgg cagattatca ggcacacttg gaaaaagtgt tcggctaatt aaaaatccat
      181 cttcaacacg gagatggatt ttgtttgttt cgttgatttt gtgtcagttt cagatgtagg
      241 tgcttattcg gacgggccgt ctgaaaatgt ttgccccaat gcaaaaaaat cactgcaaac
      301 cttcataaac ggggtttgca gtgatttttt caaatcaaac agattgaaaa cctgcgccga
      361 attgttcaga cggcattatt ttttcagttc ggacagaatg tcatctacgg ttttcttcgc
      421 atctccgaaa cacatcacgc tgttttcgtt gaagaacagt gggttttgta cacctgcgta
      481 gccggtattc atcgagcgtt tgaagacgac gacttctttt gccttccaca cttccaacac
      541 gggcataccc gcaatcgggc tgttcgggtc ggtttgggcg gcggggttga cggtgtcgtt
      601 cgcaccgatg accaagacca catcggtttc ggggaagtcg tcgttgattt cgtccatttc
      661 caaaacgatg tcgtagggga ctttggcttc ggcgagcagt acgttcatat gaccgggcag
      721 gcggccggcg acggggtgga tgccgaagcg tacttcggtg ccgtttttac gtaaaagctc
      781 ggtgatttcg gcaacggggt attgcgcttg tgcgactgcc ataccgtagc ccggggtaat
      841 gatgacattg tttgcgcctt tcagcatttc ggcaatatcg gcagctttga cttctcggta
      901 ttcccctatc tcttggctgc cggaagataa tgtgccgctg tcgctgccga aaccaccggc
      961 aattaccgag acaaacgagc ggttcatggc tttgcacata atgtaggaca gaatcgcgcc
     1021 gcttgagccg accagcgcgc cggtaacgat gagcaggtcg ttggagagca tgaagcctgc
     1081 cgctgcggcc gcccagccgg agtaggagtt gagcatggac acgaccacgg gcatatctgc
     1141 gccgccgatg gaggcaacca agtgccagcc gaatgcgagg gcaatcaggg tcataatcag
     1201 caggatgaag ccgctgccgt caatgccgac aaatacgagc agcaacacaa acgatacggc
     1261 aagtgccagt gcgttgagct tgtgtttggc gggcagttgc agcgggctgc tgctgatttt
     1321 gccgttgagt ttgccgaatg cgaccagcga gccggtaaag gttaccgcgc cgatgaagat
     1381 gcctaaatac acttcgacca gatggatggt gtgcatatcg tgcgaaacgt tgcccggcgc
     1441 gatatagctg ttgaagccga ccaaaaccgc cgctaggccg acgaagctgt gcagcagggc
     1501 aatcagttcg ggcatttcgg tcatttccac ctttttggct ttgtagatgc cgattgccgc
     1561 gccgatgagc atggcgatga tgatccagcc cagtccgtgg gtattgtcgg aaaaaacagt
     1621 tacaaaaagg gcgaccgcca taccggcgat accggaatag cagccctgtt tggcggtttc
     1681 ctgtttggac agccccgcca gtgagaagat gaataaaatt gcggcaacga tatacgccgc
     1741 tgttacgagt cctgaagaca tagaaattct ccgattttcg atgatttgtt ttcaatgccg
     1801 tctgaaaaat tgacgttcgt gttttcagac ggcatctgtt tcaagcagcc gcgacaaaca
     1861 gcccgacggt gcaggcaaat ccgcctgccc acatcagtga gcgcatagcg gctttgtcgg
     1921 cgatatagca ccagataaag gcgaggcgga acaggatgaa caggcaggca agcgtgttga
     1981 tggtcgattg cgccgcattg ccggttgcgt gtgccgtcaa aacggcggcg gcaaacggtg
     2041 caaaggcttc aaaaccgttt tgctgtgcgg cgtgggcacg ggcggctgcg ccttgcgtgt
     2101 gcgccagaaa accgcgcgga ttgtggttgt ctttaaaccg gaatccgccc gcttttttgg
     2161 catacgccgc acaaaaaagc ggcaataggc aggcaatcag aatacaccaa taggcgaaag
     2221 tcatggctta ccctttctta aacatattca gcatacgccg tgttaccgca aagccgccga
     2281 agatgttgat gccggcaatc aggatggcaa caaacgacag cagcgaaacg aagccgttgc
     2341 cctgaccgat ttgcagcagc gcgccgacga cgatgatgcc ggagatggcg ttggttaccg
     2401 acatcagcgg tgtgtgcagc gagtggctga cgttccagac gacgtagtag ccgatgacgc
     2461 aggcgagaac gaacacgata aagtggttca ggaatgctgc gggtgcgacc gcgccgaccc
     2521 acagtaccaa gacggcggcg atgacggcgg gcgcgagttt tttccacagg ggaacgggtt
     2581 ttggctcggg cttggcggca ggcacggctt tttcagacgg cgtttgctgc ggctgggcgg
     2641 aaacttgaat cggcggaggc gggaaggtga tttcgccgtc gtgggtaacg gtcatgttgc
     2701 ggataatcac gtcttcgaag tccaacgtga tttcgccgtc tttgttcggg cttaacagct
     2761 tggtcaggtt gaccaagttg gtggcgtaaa gctgggaaga ctgtccggca aggcggtttg
     2821 ccatgtcggt gtagccgatg attttcacgc cgttgccggt tacggacaat tcgcccgggc
     2881 gggtgagttc gcagttgccg cccgtcgccg ccgccaaatc gacgatgacg gagccggatt
     2941 tcatgctttc caccatttct ttggtaatca gcttgggcgc gggtttgccc ggaatggcgg
     3001 cggtggtgat gatgatgtcc acttctttcg cctgctcggc aaagagcttc atctcggctg
     3061 cgataaattc gtcgctcatc actttggcgt agccgtctcc gctgccgccc gattcttgtg
     3121 ggaagtcgag tttcaggaac ttgccgccca tcgattcgat ttgttccgcc acttccaagc
     3181 gggtatcgaa cgcgcgtacc actgcgccga gcgagtttgc cgtaccgatc gccgccaaac
     3241 ctgccacacc tgcaccaatc accaaaacct gcgcgggcgg cactttgccg gcggcggtaa
     3301 tttgaccggt gaagaaacgg ccgaaggcgt tggcggcttc aattacggcg cggtagccgc
     3361 tgatgtttgc catcgaagac aaagcgtcca aagcctgcgc gcgcgaaatg cggggcacca
     3421 tatccatcgc cagcgcgttc actttcttgg cgcgcaaggc ttcgaccaaa gcctcgtttt
     3481 ggcgcggcca caggaagctg acgatggttt gaccttcgtt caaaagcggc agttcctgtt
     3541 cggacggcgc gttgacctta taaatcaaag ggcagaccca aaccgccgct ttgtcggcaa
     3601 cggttgcgcc tgctgtttgg taagcggcat cgtccaaact tgccgccaaa cctgcaccgc
     3661 tttcgacaac ggtttcaaag cccagtttgc ccagcagggc gacggtggcg ggcgtacagg
     3721 cgacgcgggt ttcgccggat aatgactcgc gtgggatacc gattttcatc tctgaatcct
     3781 ttttcgggtt gtttatatgt atcgtgggtt aaatttaaat cggggcgggg cggagcaacg
     3841 ccgtaccggt ttaaagccga ctcacttcaa atgttaatat attttagata atccccttat
     3901 aacgaatttt catcaggctg gcaatagttg cggcattttc ccgtgttgtc cgacacatac
     3961 tgccgtttgt gtttgaaatc gttttccgat tgcagtcccg cccgccgtaa cattctttgc
     4021 cgtttcacta tataatccgc attttttgag ccgccgttat gccgcacgcc ctcgtcctcc
     4081 aatttccctc cgccgcagcc ctgccttccg acttcccctt acgcctgccc gaacctgatt
     4141 gcgccgatga aaagcgtatg cgttttatcg ttgaagaagg gttttcttta agcgaaaaag
     4201 acgcggcgtt gcttggcagc cgtcaaatcg accacgccgt gttgccggat atggatttcg
     4261 acgaactcgg tttgattgtc agcgatatgg attcgacgct gattaccatc gaatgcgtcg
     4321 atgaaattgc ggcaggcgtg ggtttaaaaa acaaagtagc ggaaattacc gagcgttcga
     4381 tgcgcggcga actcgatttc gaacagtctt tacgcagccg cgtcgcgctg ttggcgggat
     4441 tggacgaacg ggttttggcg gacgtttatg aaaacgtttt gaagctctcg cccggtgcgg
     4501 aatttttgtt ggacgaatgc aaaaggcacg atgtgaaatt cctgctggtg tcgggcggct
     4561 tcacgttttt taccgaaagg ctgcaacaac gcctcggctt cgaataccaa cacgccaatg
     4621 ttttggaaat tgaaaacggc aggctgaccg gccgtctgaa aggcagaatc atcgacgcgc
     4681 aggcaaaggc agatttgttg cgcgaatacc gcagccgcct cggattgcag ccgcatcagg
     4741 tgttggcggt gggcgacggt gcgaacgata ttccgatgct caaagaagcg ggcataggcg
     4801 tggcttaccg tgccaaaccg aaagcgcggg ccgccgccga tgcctgtatc aacttcggcg
     4861 gtttggagcg tgtacgcggc ctgttcggat aggcggatag gaaacggatg tcgtccgaaa
     4921 ggttttcaga cggcatttga acggcaggaa cgacagtggg acgcagaaaa ctttggtttg
     4981 ccctggcagc agcgggcgtt atcggcggtt tggtcggcat tgtgctgacg gaactgatgc
     5041 acttcataca gcatacggca tacggttatg gcgcggacgg cgtgtacact tcgttccgcg
     5101 aaggcgtggc acaggcttcc ggtatgcggc gcgttgccgt gctgacgctg tgcggcgcgg
     5161 tcgcaggcag cggctggtgg ttgctgaaac gtttcggcaa gccgcaaatc gaaatcaaag
     5221 ccgccttgaa acagccgttg caggggctgc cgtttctgac gacggttttc catgttctgc
     5281 tgcaaatcat aacggtcgga ctcggttcgc cgctcggacg cgaagtcgcc ccgcgcgaaa
     5341 tgaccgccgc gtttgctttt gccggcggca aacgcttggg tttggatgaa ggcgaaatgc
     5401 ggctactgat tgcttgcgct tcgggtgcgg gtttggcggc cgtgtataac gtgccgctcg
     5461 cctccacact tttcattctc gaagccatgc tgggcgtgtg gacgcagcaa gccgtcgccg
     5521 ctgcattgtt aacttcagtc atcgccaccg ccgtcgcgcg catcggcttg ggcgacgtgc
     5581 agcaatatca tccggccaac cttaccgtca atacttcatt actttggttt tccgccgtca
     5641 tcggcccgat actgggcgta gccgccgtct ttttccagcg taccgcccaa aagttcccct
     5701 ttatcaagcg cgacaatatc aaaattattc ccttggccgt ctgtatgttt gcactcatcg
     5761 gcgtgatttc cgtttggttt cccgaaattt tgggcaatgg caaagcaggc aatcaactga
     5821 cctttggcgg attgaccgat tggcaacaca gccttgggct gaccgccgtc aaatggctgg
     5881 tcgtcttaat ggcgcttgcc gtcggcgcat acggcggtct gattaccccg tccatgatgc
     5941 tcggcagtac catcgccttt gctgctgcca ccgcgtggaa cagtgttttt cctgaaatgt
     6001 cctctgaaag cgcagccatt gtcggcgccg cagttttcct cggtgtttcc cttaaaatgc
     6061 ccctgaccgc catagccttt attttggagc tcacctacgc ccctgttgcc ttgctcatgc
     6121 cattatgtac aggcatggca ggtgcagtat gggtggcaaa gaaaatggga tttaaatagg
     6181 caaaagcaaa aggccgtctg aaaccaagtt tcagacggcc ttttacaata aaattgttaa
     6241 caatatttgc aaaaacctac tgccaaaaat gcgaaactgg gggataatac cgccctgaaa
     6301 attcatccca tactgattaa accttcaaca aaggaaatcc aaatgtcttc catcaaacgc
     6361 gccctgatca gcctatccga caagacaggc gcagtcgaat ttgcccaaac cctgcacaaa
     6421 ctcggtgtcg aaattctttc taccggcggt acagcaaaac tcttggctga tgcaggcgtt
     6481 cccgttatcg aagttgccga ctataccggt tttcccgaaa tgctcgacgg ccgcgtgaaa
     6541 accctgcatc cgaaaatcca cggcggtatt ctcggtcgtc gcgatttgga cgaacacgtc
     6601 gccaagatgg aagaacacgg catcggcaat atcgacctcg tgtgcgtcaa cctctacccc
     6661 ttcgctgcca ccatcgccaa accaaactgc acgctggaag acgcgattga aaacatcgac
     6721 atcggcggcc cgaccatggt gcgctctgcc gcgaaaaact ggaaacacgt cgccatcgtt
     6781 accgacaccg ccgatttccc ggccatagct gccgaactcg aagccaacaa cggcgcattg
     6841 agcgacaaaa cccgtttcaa cctctcgcgc aaagcattca gccataccgc ccaatacgac
     6901 ggtatgattt ccaattacct gacctcgctt tcagacgacg tcttgagcgg cacgcccgaa
     6961 atcgccggat tccccggccg gttcaatcaa agctggatta aagtgcaaga catgcgctac
     7021 ggcgaaaacc cgcatcagcg cgccgcgttc taccgcgata ttgaccccgc cgcaggcagc
     7081 ctcgctgcat acaaacaact gcaaggcaaa gaattgtctt acaacaacat cgccgatgcc
     7141 gatgccgcat gggaagccgt caaatccttc gacgtgcccg cctgcgtgat tgtgaaacac
     7201 gccaatccgt gcggcgtagc catcgcctcc aataccttgg atgcctacaa actcgcctac
     7261 gccaccgaca ccaccagcgc gttcggcggc atcatcgctt tcaaccgcga agttgacggc
     7321 gcaaccgtca aacaaattac cgacaaccag tttatggaag tcctcatggc gcctaagttc
     7381 accgccgaag ccctcgaaat cgccgccgcc aagaaaaacg tgcgcgtatt ggaagtgccg
     7441 cttgaggcag gcgcaaaccg cttcgaactc aaacgcgtcg gcggcggact gttggtgcaa
     7501 acgcccgaca tccaccgcat cagccgcgcc gatttgaaag tcgtctccaa acgccaaccg
     7561 accgagcagg aatggaacga tttgctgttc gtctggaacg tcgccaaata cgtcaaatcc
     7621 aacgccatcg tattcggcaa aggcggtcaa acctacggca tcggcgcagg ccaaatgagc
     7681 cgcgtggaca gcacccgcat cgccgcccgc aaagcgcaag atgccggtct cgacctcaac
     7741 ggcgcgtgtg ccgcatccga tgccttcttc cccttccgcg acggcgtgga cgtgattgcc
     7801 gaacagggca tcaaagccat catccatccg gcaggctcga tgcgcgatca ggaagttttc
     7861 gacgcagccg acgaacacgg catcgccatg gtcgtaaccg gcatccgcca tttccgccat
     7921 tgatgcagat aaacaaggta atgccgtctg aagggctttc agacggtatt ttgcgctatt
     7981 ttgcgaaggt agggatgacg gttcgggtat tcctgacagg gtggattttc aaggtgttgt
     8041 atagggtgta ggaggattcg taaaaggtgg gatgcagggt gtgcttcagc ccgctgcatc
     8101 aaaaattttt ggagaaccgg cgggagtcgg cggttttggt ttcggcgggg acggtggaaa
     8161 tgggtaacat tgacggaatc gacggaagcg gtggactgaa gcccaccctt gtatattgga
     8221 atcaccgtat catagcaaca aaccgcccag ccgccacccg cgcccaccca aggcacacaa
     8281 ccgttgcgta gctcagggag cggcagggca acccatcgac acaaccggac agttgccgga
     8341 caacacaacc gaatgcaagg caggtttatg atgagtaccc aataccatta cgcaggtata
     8401 gtgaattaaa tctaaaccag tacagcgttg g
//
LOCUS       DAWGRZ010000083         8364 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000083 DAWGRZ010000000
VERSION     DAWGRZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.083"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(457..732)
                     /locus_tag="WHT44_001271"
     CDS             complement(457..732)
                     /locus_tag="WHT44_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ2533453.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(729..2012)
                     /locus_tag="WHT44_001272"
     CDS             complement(729..2012)
                     /locus_tag="WHT44_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HEZ2533454.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAVYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWLEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGPFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(2168..3586)
                     /gene="glnA"
                     /locus_tag="WHT44_001273"
     CDS             complement(2168..3586)
                     /gene="glnA"
                     /locus_tag="WHT44_001273"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ2533455.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDATVVLTCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGETAYFGPEPEFFVFDGIEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPTVKGGYAPVAPIDCGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            3670..3855
                     /locus_tag="WHT44_001274"
     CDS             3670..3855
                     /locus_tag="WHT44_001274"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533456.1"
                     /translation="MPSETGFASNFKPENIVSDGKIVNNADKPAHRIRIFPDRYRKRC
                     RLKAVSDGIVSICTPPL"
     gene            3886..4695
                     /gene="aroE"
                     /locus_tag="WHT44_001275"
     CDS             3886..4695
                     /gene="aroE"
                     /locus_tag="WHT44_001275"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ2533457.1"
                     /translation="MTALPRYAVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIPLKNGKLRGD
                     NTDGIGLTNDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELAQLFGIEAVPMADVNGGFDIIINGTSGGLNGQIPDIPPDIFQNCALAYDMVYGCA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAL"
     gene            4698..5399
                     /gene="mtgA"
                     /locus_tag="WHT44_001276"
     CDS             4698..5399
                     /gene="mtgA"
                     /locus_tag="WHT44_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ2533458.1"
                     /translation="MFRIIKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWMPYKRISTNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LKRMGSAELPESDTD"
     gene            complement(5602..5677)
                     /locus_tag="WHT44_001277"
     tRNA            complement(5602..5677)
                     /locus_tag="WHT44_001277"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5642..5644),aa:Phe,seq:gaa)
     gene            complement(5868..6602)
                     /gene="lptB"
                     /locus_tag="WHT44_001278"
     CDS             complement(5868..6602)
                     /gene="lptB"
                     /locus_tag="WHT44_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ2533459.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVIGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVTLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRSPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(6676..7206)
                     /gene="lptA"
                     /locus_tag="WHT44_001279"
     CDS             complement(6676..7206)
                     /gene="lptA"
                     /locus_tag="WHT44_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ2533460.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(7187..7768)
                     /gene="lptC"
                     /locus_tag="WHT44_001280"
     CDS             complement(7187..7768)
                     /gene="lptC"
                     /locus_tag="WHT44_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ2533461.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(7765..8301)
                     /locus_tag="WHT44_001281"
     CDS             complement(7765..8301)
                     /locus_tag="WHT44_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ2533462.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQA
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPGAHWFTRQHAAYITEHAGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
BASE COUNT         1952 a         2276 c         2144 g         1992 t
ORIGIN      
        1 cttacaatat aaaacaaaca aatatagtgg attaacaaaa accagtacgg cgttgtctcg
       61 ccttgccgta ctatctgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc
      121 cactataagt tgacatattg tgcgatgtcg agggctgtac cgtattttcc catcgtattt
      181 tcccgttctt tatatgagcg gcaggcatcc tcataaaaga agggataccg ataaggaaaa
      241 ataccccggc ataaaaaata tactgattaa tcaatacaaa tccaacaaac agttcatcgt
      301 attcttttct gttggcgtat aaaaaatact gatatatgcc ttcactttca tagggcgacg
      361 ctttatgtac cgtcctgtgt gttgaaacgt cggttttcct taccgcctgc cccgcttttc
      421 cctgattgct gccaactgcc gtccgaacct gaaccgtcag gtttcagacg gcattttttc
      481 tgcctgctcc cacaattgcc gtttttgtgc ggcggtcagt tttttgacgg cgatttcctg
      541 cctgagtgcc gttcctttat ccatcccgcc tgcaacgata cgcatcgcca ccggtttgaa
      601 tacgcgggta tatttcgcgc ctttaccggt tgtgtgggcg gcaagccgct gttgcggatt
      661 cgggctgatg ccgcaataga acgcgctgtt ttcacacaat atcaggtaaa cgctccagtt
      721 tgacgcgttc atctgcctgc atcctgagtt ttctccccgt tccaaggcgc aactttcagc
      781 agcagcagca tacccggtag ggcgagtgcg aaacacagtt ggaaaaacgg tacataaccg
      841 agccattcaa tcagataacc ggcaaaggaa ttgatgaccg tgcgcgggac ggcggacagg
      901 ctggtaaaca gcgcaagctg cgttgcggta aatgcgggat tggtttcacg cgccatatac
      961 gatacgaacg ccgccgtccc caaccccacg ccgaccgctt ccgcgccgat aactgccgcc
     1021 agcatcagcc tctcgcctgt gccgaccgtg tcgaaaggtc cgaaccctgc cagccataca
     1081 aaccccaaaa cggttacagc ctgcaccgcg ccgaatagcc acaaggcttt gtttacgccg
     1141 attttcagca tccacacacc gcccaagata cctgccgcca ctgccggcca cagtcctgca
     1201 tttttcgcaa tcaaaccgat gtcggtcttg ctgaaaccca tatccagata aaacggcgtt
     1261 gccaacgcgg ttgccatact gtcgccgagt ttgtaaagga agataaacag cagcacgcac
     1321 accgccgaag cgatgccctt gcgcgtaaaa aattctttaa acggctctac cacggtctgc
     1381 ttcaacgttt taggaacggc aggaggcaac acgggttcgc gcgcaagaaa cagcgtcatc
     1441 agaaggccgg gcagcataaa taatgaagtg ataacaaata cttctaacca cggcatcctg
     1501 tctgccaaca ccaaactcaa tgaaccggga atcagggcgg caatccggta ggcgttcaca
     1561 tgaaccgagt tgcccaaacc caattcttcg tctgacaaaa tctcgcgcct gaacgcatcc
     1621 aatacaatat cctgactggc ggaaaaaaaa gcgacaagca ccgacaagcc ggcaatcagc
     1681 ggcagatgat tacgggggtt taaaaaggca tagaccgcca aagccgccag caaccctgcc
     1741 tgcgtcagca gcatccaccc gcgccgccgt cccaaaacgg gcagcctgac cgcgtccatc
     1801 agcggcgacc acaaaaattt ccaagtaaac ggcagaccga ttaacgccat cagcccgatg
     1861 ctcttcaaat ccacctgctc gctgcgcaac cacgccggaa tcaggttaat cagaaagtac
     1921 agcggcagcc ccgaggtaaa ccccgtaaaa atacagacaa gcatattgcg ggaaaagatt
     1981 tgcccgataa aacctgattt cgatgcagtc atcgttcgtg cagcccaaaa aaaacaaaag
     2041 cctattgtag caaatatcgc ccgcctgctg aaaaatgccg ccccgcgccg ccggtttgcc
     2101 gttcaaatgc aaaatgccgt ctgaaactgt gttcagacgg cattgctttt ctgaaaacca
     2161 gacgtgctta caggctgtaa tacatttcaa attccagcgg gtgcggcgcc atgcggatgc
     2221 ggcgtacgtc ttcttctttg aaagcgatgt agctgtcgat ccagtctttg ctgaacacgc
     2281 cgccgcgcag gaggaattcg tggtcggctt tgagggcggc cagtgcttct tccaaagaag
     2341 cgcaaacggt cggcaccaat gcatcttctt ccggcggcag atcgtacagg tttttatcgg
     2401 cagggtcgcc cggatggatt ttgttttgaa tgccgtccaa acccgccatc aacagggcgg
     2461 caaatgccaa atacgggttg gcggtcggat cggggaaacg cgcttcgatg cggcgcgcct
     2521 tgctgctgtt cacggacgga atgcggatgg aagcggaacg gtttttggcg gagtatgcca
     2581 gtttggtcgg cgcttcaaag tgcggcacga ggcgtttgta ggagttggtg gacggattgg
     2641 taatcgcgtt caaggctttg gcgtgtttga tgatgccgcc gatgtagtag agcgcggtgt
     2701 cgctcaagcc ggcatagccg tcgcctgcga acaggttttg accgtctttc caaatggatt
     2761 ggtgaacgtg cataccgctg ccgttgtcgc ccataatggg tttgggcatg aaagtggcgg
     2821 ttttgccgaa gttgtgggca acgttttgaa tcacatattt catgtcttgg gtttggtcgg
     2881 cgcgtttgac caaagtagca aagcgcgtgc cgatttccat ttggctgccg gtgccgactt
     2941 cgctgtggtg cacttccact tcaataccga gttcttccaa aatgtttacc atcgccgaac
     3001 gcaaatcctg accgcagtca atcggtgcaa caggtgcgta accgcctttg acggtcgggc
     3061 ggtggccggt gttttgaccg tccatatgca gaccgcttgc ccacgcgccg ctttcggacg
     3121 tgatttcgta acgggttttg tgcatatcgg tttcaaattc tatgccgtcg aatacgaaaa
     3181 actcgggttc gggaccgaaa taggcggtct cgccgatgcc ggaagatttc aaataggctt
     3241 cggctcggcg ggcgatggag cgcgggtcgc ggtcgtaacc ctgaccgtcg gcgggatcga
     3301 taacgtcgca agtcaacaca acagtcgcat catcataaaa aggatcgacg aaggctgtag
     3361 acgcatcggg gcgcaactgc atatcggaag cctgaatgcc tttccagccg ccgatagacg
     3421 aaccgtcaaa cgcctgaccg ttttcgaacc actcttcggg gtcttccaac acgatgcgcg
     3481 caggcacggt aaagtggtgc tgcttgcctt tggtatcggt aaagcgcaaa tcgacaaagc
     3541 gggcttcgct ttcttcaatc aattttacgg cgtttttaat agacattttt cagctcctgg
     3601 aaaagggaaa tagcaaaata tgcggaacgc ggcggcttga cgcaaagtcg gcacgatttt
     3661 accacgccga tgccgtctga aaccggtttt gcgtcaaatt tcaagccgga aaatatagtt
     3721 tcagacggca agattgtcaa caatgcggac aagccggcgc accgcatccg gatttttccc
     3781 gaccgataca ggaaacgatg ccgtctgaag gcggtttcag acggcatcgt ttccatctgt
     3841 acgccgccgt tataaaatag ccttcccgtc ttttggaaca cgaccatgac cgccctcccc
     3901 cgctacgccg ttttcggcaa ccccgtcgcc cacagcaaat cgccgcaaat tcatcaacaa
     3961 tttgcccttc aggaaggcgt tgacattgaa tacgaacgca tttgcgccga catcggcggt
     4021 ttcgcgcagg cggtttcgac attttttgaa acaggcggtt gcggggcaaa cgttaccgta
     4081 ccgttcaaac aggaagcgtt tcatctggcg gacgagcatt ccgaacgcgc attggcggca
     4141 ggtgcggtca atacgctgat tccgttgaaa aacggcaagc tgcgtggcga caacaccgac
     4201 ggtatcggtt tgaccaacga catcacgcag gtcaaaaata ttgccatcga gggcaaaacc
     4261 attttgcttt tgggcgcagg cggcgcggtg cgcggcgtga ttcctgtttt gaaagaacac
     4321 cgtcctgccc gtatcgtcat tgccaaccgt acccgcgcca aagccgagga attggcgcag
     4381 cttttcggca ttgaagccgt cccgatggcg gacgtgaacg gcggttttga tatcatcatc
     4441 aacggcacgt cgggcggtct aaacggtcag attcccgata ttccgcccga tatttttcaa
     4501 aactgcgcgc ttgcctacga tatggtgtac ggctgcgcgg caaaaccgtt tttagatttt
     4561 gcacgacaat cgggtgcgaa aaaaactgcc gacggactgg gtatgctagt cggtcaagcg
     4621 gcggcttcct acgccctctg gcgcggattt acgcccgata tccgccccgt tatcgaatac
     4681 atgaaagccc tataaacatg ttccgcatca tcaaatggct gattgccctg cccgtcggca
     4741 tctttatctt tttcaatgcc tatgtgtacg gcaacatcat tacctaccgc gccgtcgcgc
     4801 cccatcggac tgcctttatg tcgatgcgga tgaagcagtt tgaacaggaa ggtcgcgatg
     4861 tcgcactgga ttaccgctgg atgccctaca aacgcatttc caccaacctg aaaaaagccc
     4921 tgattgcttc cgaagatgcc cgtttcgccg ggcacggcgg cttcgattgg ggcggcattc
     4981 aaaacgccat caggcgcaac cggaacagcg gcaaagtgaa ggcgggcggc tcgaccatca
     5041 gccagcagct tgccaaaaac ctgtttttaa acgaaagccg cagctatatc cgcaaaggcg
     5101 aagaagcggc gattaccgcg atgatggaag ccgttaccga caaagacagg atttttgaac
     5161 tgtatttaaa ctcaatcgaa tggcactacg gcgttttcgg cgcggaagcc gcgtcccggt
     5221 atttttatca aatacccgcc gccaagctga ccaaacagca ggcggcaaaa ctgacggcgc
     5281 gcgtccccgc cccgctctac tacgccgacc atccgaaaag caaacggctc cgcaacaaaa
     5341 ccaatatcgt gctcaaacgc atgggttcgg cagagttgcc tgaaagcgac acggactgag
     5401 tgccgccgca atataaaatg ccgtctgaaa tatcattcag acggcatatg ctttctgcct
     5461 ttaccctctt ctccaaaacg caacggggcg gtcattatca tttttgccct tctacataac
     5521 ggtcagacac aaaacgtgtc cgacacggac aacttcccaa aaacggcagg caaaaaaaat
     5581 aaccgcttca aggcggtttt ttggtggcca gaggcggaat cgaaccgccg acacacggat
     5641 tttcaatccg ttgctctacc aactgagcta tctggccttt gtctgtctcg ttaagagatg
     5701 cgtattaaac caaattacgg gacgacatgc aagcatatcg ggcaatattc tttgaaaaca
     5761 ggtttaagtc aatgttttaa atttgaatta atttatgtaa aaacaacata aatccgtatt
     5821 tttgccgcct gcccgacgat attaggtcgc ccgtctgaaa aatattttca atatttgaag
     5881 ttcttaccca gataaacaga acgaacctgt tcgtttccga ccaaatcatc aggttttccc
     5941 gatgccaaca ccgtgccgtc tgaaataatg taggcccgat cgcagatgct gagggtttcg
     6001 cgtacgttgt ggtcggtaat cagtacgccg ataccgcgcg atttgaggaa accgatgatt
     6061 ttctggatgt cgatgacggc aatcggatcg acgccggcaa aaggttcgtc caacaaaata
     6121 aaatgcggtt tcatggcgag tacgcgggcg atttcgacgc gccgccgttc gccgccggac
     6181 agcgacggcg cggggctgcg gcgtaagtgt ccgatattga ggtcggcgag cagtttttcg
     6241 atttccctgt cgatttgatt tttatctttg gttctgattt ccaagatggc gcggatgttt
     6301 tgttcgacgg tcattttgcg gaatatcgag gcttcctgcg gcaggtagcc gacaccgagg
     6361 cgggcgcgtt cgtgtatggg caggtggcgc aattcttgtc cgtctagggt tacgctgcct
     6421 gcgtcggcgg cgatgagtcc gacaatcatg tagaagctgg tggttttacc cgcaccgttg
     6481 ggcccgagca gtccgatgac ttcgccgctt tcgatttcga gggagaagct tttaacgact
     6541 tggcgttttt tgaaactttt ttgcaggttt tgaacaacaa ggcggctgac gtttgcactc
     6601 ataaatatct cttcaatatc ttcggtcggc atatgccgtc cgaaccgggt ttatatttca
     6661 gacggcattt tgggattatt cggatttttg cgtactcgaa ggctggataa cgacgctgac
     6721 cctgccggat ttggaagcgg attttgcgcc ggattttgtg ctgccgctga tggtatagac
     6781 ttcggttttg gtgttgtatg taatcaccgc accttcggcg acatcgccgc cgcgctgtac
     6841 tttggcatta ccggttaaga ctacggtgct gcctgcagat gaataagcaa cgttgtttgc
     6901 ctgtccgcgc accgtgcctt tgccgccgtc caatgtctgg ctgaagcgga ctggcgaacc
     6961 ttccgccctc acggattcgc cgcctttgcc gccgcgtgta acattgacgc gggcggcgga
     7021 aatattgagc gtaccctgtc tgatgacgac gtttccgctg aatgtggtgc tttggttggc
     7081 ttgatcgagc gaaccttggt cggcctcaat ctgaataggc tgcctgctgt cgctttgaag
     7141 ggcaaaagcg ggggacgccg aaaaaaatgc tattaaaaca aatagcttac atatcttttg
     7201 tatcataaat cgtggctttc actttagatg agaagttcaa catgcctgtt ttgtggtcgt
     7261 aagtcatgcc gcccgcctga ccgtgcgatg caccatattg gaaactgaca ggcgtatcgg
     7321 tttgggcata ttgagattcg gtatcgacgt gcagcttttc ggcttcaact ttacccgcct
     7381 gccgtttgcc gtcggcggtt ttggtcagca caacgttgtt tttaaaaaga acctgtttgt
     7441 tttcggtatg gtaaacggct tcgtcgctgc cgacttcgta caacaacctg ccttcttgga
     7501 agaacacgag atgcggcgaa tcaaaatgga tgtcgctgct ttccggaaac tgtttcgcgc
     7561 ccttcgcgct caaatgttct ttcaagtatc cctgttcgtc aaaccgcctg ccgtccaagc
     7621 cgtccattgt gtattgcggt tcgtcgggat tgagcctgac ttcttcaatc tcgacttcgc
     7681 tgatacgacc caaccatgcc gacaggctgc ccaaggcaac cgccaatatc aatgggaacg
     7741 caattccgta ccgccatctt actttcattt gatgtactcg ttcaaagccg cgcccaaagt
     7801 cccttgcgcc tgcataatca ggtcgcacac ttcgcgcacc gcgcctgcgc cgcccgcgtg
     7861 ttccgtgata taggcggcgt gttgccgcgt aaaccaatgc gcgccgggga cggcaaccgg
     7921 caatccgcag cgcaccatta ccggcaaatc gaccacgtcg tcgccgacaa aggcgcactc
     7981 ggcttcttcc acgcccgcct gcgcgcgcaa ttcttcatag gcggcacgtt tgtcgctgat
     8041 acctttgaaa tagtaattta tgcccaactg tttgacgcgg atgccgacgg agggcgcgtc
     8101 ccggcccgtg ataatcgcag tctgcacgcc gcttgcctga agcattttca gaccgtgtcc
     8161 gtccagtgtg tgaaacgatt tgatttcttc gccgttatcg cggataaaga tgcgcccgtc
     8221 ggtcaaaacg ccgtccacat ccaggatcaa cagtttgatt ttggcggcgc gcgcctgtaa
     8281 ttcgggagaa attgcctgca tgggatttcc ttgttttcag acggcatatc ttaccacaaa
     8341 tgccgtctga aacggctgca acgc
//
LOCUS       DAWGRZ010000084         8333 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000084 DAWGRZ010000000
VERSION     DAWGRZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.084"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            70..2184
                     /locus_tag="WHT44_001282"
     CDS             70..2184
                     /locus_tag="WHT44_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ2533463.1"
                     /translation="MFLFSDGLQSINNNNRRKRIVKNAYIPSRGIRKIPHLSTLLPEF
                     HICKDGKEAEAVVGWGLRPTTHKARAFAAEHQLPFIALEDGFLRSLGLGVAGYPPYSI
                     VYDDIGIYYDTTRPSRLEQLILAADTMPSETLAQAQQAMDFILQHHLSKYNHAPELSD
                     DHPLRSPSKPETVLIIDQTFGDMAIQYGGADASTFELMFQTALNENPQADIWVKTHPD
                     VLCGKKQGYLTQLAQQHRVHLLAEDINPISLLQNVDKVYCVTSQMGFEALLCGKPLTT
                     FGLPWYAGWGVSDDRHPEINRLVQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSL
                     FDVIDYLATVKRKNDKLRGELYCVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVK
                     LSDDARILAWGNGKEAIVRFAEQHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMSI
                     YFNAETPSRLEYILQNQNFDDQDFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTV
                     ILVPGQVEDDASIRYGSPQIYRNLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISP
                     EDAARYADQTAEQADILTCLQYADEIHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLT
                     QDLLPIPRRSRRLELWQLIAGTLIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNN
                     GLHRGCFAKKLGKIKQLYRSFK"
     gene            2321..3580
                     /locus_tag="WHT44_001283"
     CDS             2321..3580
                     /locus_tag="WHT44_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide modification protein
                     LipB"
                     /protein_id="HEZ2533464.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSNSSTPVI"
     gene            3947..5353
                     /locus_tag="WHT44_001284"
     CDS             3947..5353
                     /locus_tag="WHT44_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ2533465.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQSKLAQYALTGELSERDPFQAARYAKAAAEKNHPEALKIMGD
                     LYRYGLGIKADNHIAQDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            5571..6785
                     /gene="gltS"
                     /locus_tag="WHT44_001285"
     CDS             5571..6785
                     /gene="gltS"
                     /locus_tag="WHT44_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ2533466.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     IAAIVLFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGVSHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            6884..7900
                     /locus_tag="WHT44_001286"
     CDS             6884..7900
                     /locus_tag="WHT44_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ2533467.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            7911..8108
                     /locus_tag="WHT44_001287"
     CDS             7911..8108
                     /locus_tag="WHT44_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533468.1"
                     /translation="MNTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAV
                     RLGENGFTEEQMENIANLFGR"
     gene            8126..8275
                     /locus_tag="WHT44_001288"
     CDS             8126..8275
                     /locus_tag="WHT44_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533469.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVAYADVSTHRTIQKTS
                     PYVPS"
BASE COUNT         2142 a         2420 c         1927 g         1844 t
ORIGIN      
        1 aaagacttgg caggcaaagt attgtctgaa gcggtaacgt tttaaaaata attcaggccg
       61 tctgaaagaa tgttcctctt ttcagacggc ctacaatcca ttaataacaa taatcgacga
      121 aaacgcattg tgaaaaacgc ctacatcccc tctcgcggca tccgcaaaat cccccatctc
      181 tccaccctat tgcctgaatt tcatatctgc aaagacggga aagaagcaga ggctgttgtc
      241 ggctggggtt tgcgcccgac gacacacaaa gcgcgtgctt ttgccgctga acaccagctt
      301 ccctttattg ctttggaaga cggcttttta cgatcgctcg gactgggtgt cgccggttat
      361 ccgccctact ctatcgtcta tgacgacatc ggcatctact acgacaccac acgtccttcg
      421 cgtttggaac aactgattct tgccgccgat accatgccgt ctgaaacctt ggctcaggcg
      481 cagcaggcga tggatttcat cctgcaacac cacctgtcca aatacaacca cgcgcccgaa
      541 ctttcagacg accatccttt acgttcccca tccaaacccg aaaccgtcct catcatcgac
      601 caaaccttcg gcgatatggc catccaatat ggcggcgcag acgcctctac gtttgaactg
      661 atgtttcaga cggccttaaa tgaaaacccg caagccgata tctgggtaaa aacccatccc
      721 gatgttttgt gcggcaaaaa acaaggctat ctgacccaac tggcgcagca acaccgcgtc
      781 catcttttgg cagaagacat caatccgatt tctttgttgc aaaacgttga taaagtttat
      841 tgcgttacct cgcaaatggg ttttgaggcg cttttgtgcg gcaaaccgct gaccactttc
      901 ggcctgccgt ggtatgccgg atggggtgta agcgacgacc gccatcctga aatcaaccgc
      961 cttgttcaaa cccaacgccg cgccacccgc aacttgctgc agctcttcgc cgcagcctat
     1021 ctgcaataca gccgctacct caaccccaat accggcgaag caggcagcct ctttgatgtc
     1081 atcgactatc tggcgacggt caaacgtaaa aacgacaaat tgcgtggcga gttatattgc
     1141 gtcggtatgt ctttgtggaa acgcgcggtt gccaaaccgt tctttaacgt accctcttgc
     1201 cgtctgaaat ttatctcttc cacccaaaaa ctggcaaggg tcaaactgtc cgacgatgca
     1261 cgcatcctgg cttggggcaa cggcaaagag gccatcgtcc gctttgccga acaacaccac
     1321 atccccctgc tgcgcatgga agacggcttt atccgctcgg tcggactcgg ctccaactta
     1381 gtgccgccgc tgtcgctcgt taccgacgat atgagcattt atttcaatgc cgaaaccccg
     1441 tcccgtcttg aatacatcct acaaaaccaa aacttcgacg atcaagactt tcagacggcc
     1501 ttgaagctgc aaaaaatgct gaccgaaaac cacatcagta aatacaacgt cggcagctca
     1561 gacttcaccg ccccgtcaac cgacaaaacc gtgatcctcg ttcccggcca ggttgaagat
     1621 gatgcgtcta tccgctacgg ttcgccccaa atctaccgca atctggattt gctccgtacc
     1681 gtacgcgaac gaaaccccaa tgcctatatc atctacaaac cgcatcccga tgtagtcagc
     1741 ggtaaccgca tcggccatat ttcccctgaa gatgctgcac gatatgccga ccaaaccgcc
     1801 gaacaagccg acatcctgac ctgtctccaa tacgcagacg aaatacatac catgacttcg
     1861 ctgaccggtt ttgaagcctt gttgcgcggc aaaaaagtca gctgctacgg cctgcctttt
     1921 tacgcaggct gggggcttac ccaagatctg ctccccatcc cgcgccgtag ccgcagactt
     1981 gagctttggc agctgattgc cggcacgctc atccactatc ccgactacat ccaccccgaa
     2041 acccatcagg ccataaatgc agaaaccgca gcccaaatcc tgatacgaca aaaaaatatg
     2101 caaaaaaaca acaacggatt acatcgcggg tgctttgcca aaaaattagg taaaatcaaa
     2161 caactatatc gatctttcaa ataaatacca tcaaagttaa cgatgcgtca taaacttgcc
     2221 tctattgcgg catcattgcc tttgcatcgt taattctctt ggcgtatgct tgaaagttca
     2281 acctaaaact attacataaa aaacaaaacc acattgcaac atgaaacaga ccgtcctcaa
     2341 aaataacctg caaaacctgc ttgaaagcgc agaaaatatc ctgctgcttc aaggccctgt
     2401 cggcgatttt tttctgcgcc ttgccgactg gctgactgca aacggcaaaa ccgtacataa
     2461 attcaacttt aatgcaggcg acgactattt ttatccgccc actcaagcgc ataccgttgt
     2521 ttttaacgac aactacgatg cctttcctga gtttttgcaa gaatacatca ctcaacatca
     2581 catccaggcc gttgtctgct ttggcgacac acgcccttat cacgtcattg caaaacgcat
     2641 tgcaaacgaa aaccaagcca gtttctgggc gtttgaagaa ggctatttcc gcccctacta
     2701 catcacctta gaaaaagacg gcgtcaacgc attttccccg ttgccgcgcc gtgccgactt
     2761 ttttcttgaa caattcccta agcttgccca gcaagaatat aaagcgccaa cgccggtaca
     2821 cggcggtttt acgcccatgg caaaaaacgc tatccgttac tatatcgagt tgttccgcaa
     2881 tccacgcaaa taccccgact acatccacca ccgcgcaccc aatgccggcc attacctcaa
     2941 accgtggtcg ctctccatcc tcaagcgttt gaactactat attgaagaca tccaaatcgc
     3001 caaacgtgtg gaagcaggca aatacggcaa gttttttatt gttcccttac aggtattcaa
     3061 cgacagccaa gtccgtatcc attgcgactt tcccagcgtc cgcagcttcc tgctccatgt
     3121 tttgagttca tttgccgagc acgcgcctgc cgataccaac atcatcatca agcatcatcc
     3181 gatggaccgc ggttttatcg actactggcg cgacattaaa cgctttatca aagaacaccc
     3241 cgaactcaaa ggccgtgtga tttatgtcca tgatgtcccc ctgcccgttt tcctgcgcca
     3301 cggtctcggc atggtcacca tcaacagcac cagcggcctg tccggactga ttcacaatat
     3361 gccagttaag gttctcggcc gtgcctatta tgatattccc ggcattactg accaaaatac
     3421 cttggcagaa ttttggaatc atccgacacc gcctgacaaa gagctgttcc atgcctaccg
     3481 aatgtaccac ctcaacgtga cccaaattaa cggcaacttc tacagtcagg tgtttttccc
     3541 caacaaaaaa acctccaact cttccacacc agtaatctga cttagcgaag gaagttcagg
     3601 ccgtctgaaa acatttcaga cggcctgaaa caatcaatac cttagctact gccatgtaaa
     3661 taaaacacaa aaatctgcat ttatcattaa caataaatta caaaaacagt ataatgaccg
     3721 agctgccatg agcgcatacc gactcaacct gagccctttg taacacacaa aatatggata
     3781 tatccctagg caaaacaata taacaagcca aacatcctaa agataagccg gcaaggcaat
     3841 acactctata aaactatgcc gagcaaaatt tttacaaagc cctcaaccgg tatcgccgcc
     3901 catatgccgc agcatccgtc ttccacttta tatccgcccg caaaccatga ccgccgctcc
     3961 tgatatcctc taccggcaag ccgccgccct tttggaacaa tccaataccg cccaagccct
     4021 gcccctgttg caacaggcgg cagagcaagg ttatgcggaa gctgctttcg tattgggcaa
     4081 ccatctgctg caaaacggcc aaccggagca ggcactttca tggttggaag ccgccgcggc
     4141 ccaacgccat cccaaagcac tcttctccct gctgcaacaa cgcgaacaca acggcacccc
     4201 gaccggacag cttctcaacg actatgcctg gctgggtgag caggggcact cagaagccca
     4261 attaatcctc atgcgttacc acgcgcaacg caacgatcca caatcgctct actgggcgga
     4321 acttgctgcc gcccgatatg ccgcacctgc gtattaccat ctggcacgcc atcatcaacg
     4381 ccaaggcgac gttgaaacag ccatcgaaca atacgaaaaa gcggcagcac tcggcgtaac
     4441 tgccgcctgc tggcaacttg gtcaaatcta cttctacggt acaggtgtca gccccaacca
     4501 cgcacaagcc gaacactatc tcgaaccagc cgcacaagcc ggccacatcg ccgcacaaac
     4561 gctgctggct gaccttcttg ccgcccaacg caaacctgaa gccttggaat ggtatcgtcg
     4621 tgccgccgat aaggaacaag cggaagcaca gtctaagctg gcccaatacg ccctgaccgg
     4681 cgaactttcc gaacgcgatc cgttccaagc ggcacgatat gccaaagccg ctgccgagaa
     4741 aaaccatcct gaagccctga aaatcatggg cgacctctac cgctacggtc tcggtatcaa
     4801 agccgacaac catatcgcgc aagattacta ccaccgtgcc gccgcgctgg gttctgccgc
     4861 cgcagcacaa aaactcatca gcgacgccgc gctgtaccat ccgcaacaat acgaacaaat
     4921 caaaactgcc gccctgcaac aacaacaaac cgaaaccatc taccgtttgg cggaagcaca
     4981 agcctgcgcc atcggccgtc ccgccgacta caatgccgcg cgaaaaaatt acatggaagc
     5041 tgccgggttc caccataaaa acgcagcggc agccttaggc cgcatctacc attacggcct
     5101 cggtacggcg caagatcctc gggcggctgc acactggtac gccattgctg ccgaacaaaa
     5161 ccacccttcc gcccaatacc acctcgcctg tttttactat cacgggcaag gtgtcggctg
     5221 tcatgttccg accgcctgct actggctgca ggccgccatc ggcaacggcc acacttcggc
     5281 cgaatcatta atatccctat tagaacaatg gcgacgcgaa gcacaccatg ccatcggaca
     5341 aaaggccgtc tgaaaagatt tacactcgca ttttttgaca atctttaact attcccctaa
     5401 tatttgccag ttatttttca cggacacgcc attgttttca tttctttctg aaaacacctt
     5461 gtccgcgcat caataccatg acactcggcg gataacgcca agcgttgaaa cacactacat
     5521 ccggaacaaa aacggatgct cggaaaaata tttctaggag gtgaaacaac atggaatggg
     5581 aattcaacag ttattacaca ctgattgccg ccacgctcgt gttgctggtt ggtaaatttc
     5641 tggttcaaaa aatcaaattc ttacgagact tcaatattcc cgagccggta gccggcggtt
     5701 tgattgccgc tatcgtcctg ttcgccctgc acgaggcgta cggcgtgagc ttcaaatttg
     5761 agaaaccgct gcaaaatgcg tttatgctga tttttttcac gtccatcggc ttgagcgcgg
     5821 atttttcccg tttgaaggcg ggcggtttgc cgctggtggt ttttaccgcg attgtgggcg
     5881 gatttatctt ggtgcaaaac tttgtcgggg tcggactggc tacggctttg ggtttggatc
     5941 cgctcatcgg tctgattacc ggttcggtgt cgctgacggg cggacacggt acgtcaggtg
     6001 cgtggggacc taattttgaa acgcaatacg gcttggtcgg cgcaaccggt ttgggtattg
     6061 catcggctac tttcgggctg gtgttcggcg gcctgatcgg cgggccggtt gcgcgccgcc
     6121 tgatcaacaa aatgggccgc aaaccggttg aaaacaaaaa acaggatcag gacgacaacg
     6181 cggacgacgt gttcgagcag gcaaaacgca cccgcctgat tacggcggaa tctgccgttg
     6241 aaacgcttgc catgtttgcc gcgtgtttgg cgtttgccga gattatggac ggcttcgaca
     6301 aagaatatct gttcgacctg cccaaattcg tgtggtgtct gtttggcggc gtggtcatcc
     6361 gcaacatcct cactgccgca ttcaaggtca atatgttcga ccgcgccatc gatgtgttcg
     6421 gcaatgcttc gctttcgctt ttcttggcaa tggcgttgct gaatttgaaa ctgtgggagc
     6481 tgaccggttt ggcggggcct gtaaccgtga ttcttgccgt acaaaccgtg gtgatggttt
     6541 tgtacgcgac ttttgttacc tatgtcttta tggggcgcga ctatgatgcg gcagtattgg
     6601 ctgccggcca ttgcggtttc ggcttgggtg caacgccgac ggcggtggca aatatgcagt
     6661 ccgtcacgca tactttcggc gtgtcgcata aggcgttttt gattgtgcct atggtcggcg
     6721 cgttcttcgt cgatttgatt aatgccgcga ttctcaccgg ttttgtgaat ttctttaaag
     6781 gctgattttc cgcctttccg acaaagcacc tgcaaggttt accgcctgca ggtgcttttg
     6841 ctatgatagc cgctatcggt ctgcaccgtt tggaaggaac atcatgtatc ggaaactcat
     6901 tgcgctgccg tttgccctgc tgcttgccgc ttgcggcagg gaagaaccgc ccaaggcatt
     6961 ggaatgcgcc aaccccgccg tgttgcaagg catacgcggc aatattcagg aaacgctcac
     7021 gcaggaagcg cgttctttcg cgcgcgaaga cggcaggcag tttgtcgatg ccgacaaaat
     7081 tatcgccgcc gcctacggtt tggcgttttc tttggaacac gcttcggaaa cgcaggaagg
     7141 cgggcgcacg ttctgtatcg ccgatttgaa cattaccgtg ccgtctgaaa cgcttgccga
     7201 tgccaaggca aacagccccc tgttgtacgg ggaaactgct ttgtcggata ttgtgcggca
     7261 gaagacgggc ggcaatgtcg agtttaaaga cggcgtattg acggcagccg tccgcttcct
     7321 gcccgtcaaa gacggtcaga cggcatttgt cgacaacacg gtcggtatgg cggcgcaaac
     7381 gctgtctgcc gcgctgctgc cttacggcgt gaagagcatc gtgatgatag acggcaaggc
     7441 ggtgaaaaaa gaagacgcgg tcaggatttt gagcggaaaa gcccgtgaag aagaaccgtc
     7501 caaacccacg cccgaagaca ttttggaaca caatgccgcc ggcggcgatg cgggcgtacc
     7561 ccaagccgca gaaggcgcgc ccgaaccgga aatcctgcat cctgacgacg gcgagcgtgc
     7621 cgataccgtt accgtatcac ggggcgaagt ggaagaggcg cgcgtacaaa accagcgtgc
     7681 ggaatccgaa attaccaaac tttggggagg actcgatacc gacgtgcaaa aagagttggt
     7741 cggcgaacaa cgcaagtggg cgcaggaaaa aatcagcaac tgccgacaag ccgccgcgca
     7801 ggcagaccgg caggaatacg ccgaatacct caagctgcaa tgcgacacgc ggatgacgcg
     7861 cgaacggata cagtatcttc gcggctattc catcgattag gggcaaaccg atgaataccg
     7921 tcccaaaaag caggattccc gtcaaaccgc tgcccgaaaa aaccacagac gaagccaaag
     7981 tcgaaaaatg gcggcagctc ggtgcggaac acggtttgtc gggcgaatgg gcagttgccg
     8041 tcagattggg cgaaaacggt tttaccgaag aacagatgga aaatatcgcc aacctgttcg
     8101 gcagataaag agaaaattga cggaaatgcc gtctgaaacc ctgttatcgg tttcagacgg
     8161 cattttgacc aatacggtac gcaggcgcaa aacagccggc ttttcctgtg ttgcctatgc
     8221 tgatgtttca acacacagga cgatacaaaa aacgtcgccc tatgtgccgt cctgattcgg
     8281 aagggttacg ctccttccaa atatagtgga ttaacaaaaa ccggtacggc gtt
//
LOCUS       DAWGRZ010000085         8246 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000085 DAWGRZ010000000
VERSION     DAWGRZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8246
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.085"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(147..1079)
                     /gene="cysK"
                     /locus_tag="WHT44_001289"
     CDS             complement(147..1079)
                     /gene="cysK"
                     /locus_tag="WHT44_001289"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ2533470.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1273..1878)
                     /locus_tag="WHT44_001290"
     CDS             complement(1273..1878)
                     /locus_tag="WHT44_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533471.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(1875..2726)
                     /gene="dapF"
                     /locus_tag="WHT44_001291"
     CDS             complement(1875..2726)
                     /gene="dapF"
                     /locus_tag="WHT44_001291"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ2533472.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAEWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQVVGRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVVLPGGTLYIEWACGGDVMMTGPAEAVF
                     EGELAYS"
     gene            2863..3159
                     /locus_tag="WHT44_001292"
     CDS             2863..3159
                     /locus_tag="WHT44_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533473.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3173..3841
                     /locus_tag="WHT44_001293"
     CDS             3173..3841
                     /locus_tag="WHT44_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ2533474.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(3914..6037)
                     /gene="pnp"
                     /locus_tag="WHT44_001294"
     CDS             complement(3914..6037)
                     /gene="pnp"
                     /locus_tag="WHT44_001294"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ2533475.1"
                     /translation="MMFDKHVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVLVA
                     VTTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRP
                     LFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNG
                     VYVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAIN
                     AINEFADEVNPELWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWS
                     AVKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTS
                     VLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEV
                     GRMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCL
                     SLLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQ
                     MDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKI
                     REVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGK
                     VYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEV
                     DDRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6271..7134)
                     /locus_tag="WHT44_001295"
     CDS             complement(6271..7134)
                     /locus_tag="WHT44_001295"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ2533476.1"
                     /translation="MSEIGLVKIYFGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRAKAVRLYTEAAEYAKSRGIIICDTKFEFGLDENGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7333..8196
                     /gene="rfbD"
                     /locus_tag="WHT44_001296"
     CDS             7333..8196
                     /gene="rfbD"
                     /locus_tag="WHT44_001296"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ2533477.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     VFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFIR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSATIIRLLQHSNPVRGIYHYAGSKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         1984 a         2387 c         1904 g         1971 t
ORIGIN      
        1 atttccctca agcgatgcgg cgacaccaac acctgattaa aacacaaccg gcgttttcgg
       61 catatatgca aaaacgcatt ttccggcacg gcgttacacc gataccgaaa aatgcgtctg
      121 aatgtttcga caatccgatt aaagcattat gccaaatctg caaaaagtgg cgtagagaga
      181 tagcgttcgc cataagaagg cagcagcacg actatcagct tgccttcgtt ttcaggctgt
      241 ttggcaagct gcaacgcact ccaaaccgcc gcaccggaag aaatacccac caaaatgcct
      301 tctttttccg ctattgcgcg ggcggtttca aaagccgctt cgttcgacac tttggtaatg
      361 ctgtcgtaaa ttttggtatt caaaacggtc ggaataaagc ctgcgccgat gccttgaatc
      421 gggtgcgggc ctttttcgcc gccgctcaat acgggtgaag cttcaggctc gacggcaacc
      481 actttaactt cgggtttgta ttttttcaac acttcgccca cgccggtaat cgtaccgccc
      541 gtgccgacgc cggcaacgaa gacatcgact ttgccgtccg tatcccgcca aatttcctcg
      601 gcggttgttt tgcggtggac ttcggggttt gcctcattgt cgaactggcg cggcataaaa
      661 taagtgtcgg gatgcgcgtc caccaaggat ttcgctttgg caatcgcgcc cgccatacct
      721 tcggcggcag gggtcagaat cagctccgca ccaaacgcgc gcaacagcat tttgcgctcc
      781 ttactcatgc tttccggcat ggtaatcgcc agcttgtagc cgcgtgcggc acataccatt
      841 gccaaaccga cacccgtatt gccgctggtt gcttcgacaa tgacggtgtt tttgttgatt
      901 ttgcccgctt tttcggcacc ctcaatcatt gcttcggcaa tgcggtcttt gacgctgctg
      961 cccggattga aaaattccag tttcacggca acctctgcct tcaaaccttc ggtcagacgg
     1021 ttcagtttga ccaaaggcgt gttgccgatt agttcggtaa tgctgtttgc aattttcatc
     1081 ttctgctcct gaataataag gtttcgacag taaggcgcaa tccccatagg catcctgtcc
     1141 gattgcaggg gatttgcgcc gaaacataaa caaatttcgg atatggcttt atcctaaacc
     1201 tatgccgcct aaaaaaccaa tatccaaaat ttctttcttt ataacttttt gttccgatac
     1261 ggccgcaccg tattatcttc caatttctga aagcagcagc accagtaccg agacctgcat
     1321 caacgctgcc gtcaacccgc cgagtcgcag ctgcgagccg ccgaacgttg acggcggctg
     1381 ccggtctata ccgcagcggt ggacataggc aaccgccatc agcacaaact gcaacacgaa
     1441 ccaataaacc ggcttcagcg cataaagttt gagcagtgcc gcaaacacaa acacttgaac
     1501 cgttcccgaa aagcaataat gcaccaacgc acatattccg acaaaagcaa gcgtcatcga
     1561 tacattactg acggcaaaaa gcccgagcag cggatgttcg gggtctgcct gccatccgtt
     1621 tccatctgtt ttccggttcc aatgcccgat gaaaaaaaat atgctgccca aagtcaggta
     1681 aaaatggggg atgaacaagg gcgcggcgcg ggtcattccc catatgccgg gcatcagctt
     1741 tgcccccaaa accgatatgc ccagccacag cataatactc gcccaaaacc attgcaacat
     1801 tcctgcgcgg aatatcagaa acagaaaaac cgccgcatac agtaaggcag acaaaaaatc
     1861 caaatgcagc aaaatcatga atacgccaac tcaccttcaa acaccgcttc cgcagggccg
     1921 gtcatcatca catcgccgcc gcaggcccat tcgatatata aagtcccgcc cggcaaaacc
     1981 acctctaccg ttttcccttc atccaacagc cccagacgga tacccgccac cacagccgca
     2041 cacgcgcccg taccgcaagc ttgggtttcg cccacgccgc gctcgaacac gcgcaaacga
     2101 atcgcggttc ggccgacaac ctgcataaag ccgacattga cgcgttcggg aaactgcctg
     2161 tgcggttcga taagcgaacc ggtttcgcgc accggcgcgc attccacgtc atcgaccaca
     2221 atcaccgcat gggggttgcc catattgacg cagctgacag gctgaatgcc ggattcgaga
     2281 tgcaccccgt aaatacaggc atcatccccc tcgcccgatt cggggacaaa cggtatttca
     2341 gacggcataa acttcggttt gcccatattg accgtaacca taccgttatc ggacaatttc
     2401 ggaaaaataa cgccatttgc cgtttcaaca caaatttctt tcttatcggt caaacccttg
     2461 tctgcaacaa aacgggcaaa acaacgcgct ccgttgccgc attgcccgac ctcgctgccg
     2521 tcggcattga aaatacggta acggaaatcc acgccttcgg tttccgaacg cccgaccacc
     2581 aaaagctggt cgaagcccac gccccggaag cggtccgccc attccgcaat cggcgcgtcc
     2641 tcgggggtaa aatcctgact gaccgcgtca atcaccataa aatcgttgcc caaaccgtgc
     2701 attttggtaa atttcaaagt gttcatattg ttccgaaatc ccgtatcgtc tgttgcttcc
     2761 gaatccgaaa tcccccgcac cgaagggaaa tgcttccatc tcccgccgcc tgaggattaa
     2821 aatcagccat tatactgttt gttacccctt acaagaaaca ccatgttccg catattactg
     2881 ttgcttttcc cttgtgcaat ccttgccgcc tgcacccgaa acataccggc aaacccggcc
     2941 gtttcaaatt gcgcgcatct tcaaaaccgc accatcctgt attttgacgc acacggcaaa
     3001 cccgccgacg gcgcagtaaa gggcggattt tacaaaatta tcgaaataca gggtgcaaac
     3061 tgcatcctta ttcaagactt taacagcacg ggcaatgccc tcggcggatc ctatgtgccg
     3121 gactgccgca aatcatccct tttcccgaaa aatatttgac ttcaaactgc ctatgaacca
     3181 cgacatcact ttcctcaccc tgttcctact cggtttcttc ggcggaacgc actgcatcgg
     3241 tatgtgcggc ggattaagca gcgcgtttgc gctccaactc cccccgcata tcaaccgctt
     3301 ttggctgatc ctgctgctta acacaggacg ggtaagcagc tatacggcaa tcggcctgat
     3361 actcggatta atcggacagg tcggcgtttc actcgaccaa acccgcgtcc tgcagaatat
     3421 tttatacacg gccgccaacc tcctgctgct ctttttaggc ttatacttga gcggtatttc
     3481 ttccttggcg gcaaaaatcg agaaaatcgg caaaccgata tggcggaacc tgaacccgat
     3541 actcaaccgg ctgttaccca taaaatccat acccgcctgc cttgcggtcg gaatattatg
     3601 gggctggctg ccgtgcggac tggtttacag cgcgtcgctt tacgcgctgg gaagcggtag
     3661 tgcggcaacg ggcgggttat atatgcttgc ctttgcactg ggtacgctgc ccaatctttt
     3721 agcaatcggc attttttccc tgcaactgaa aaaaatcatg caaaaccgat atatccgcct
     3781 gtgtacggga ttatccgtat cattatgggc attatggaaa cttgccgtcc tgtggctgta
     3841 agccccattc attcccgata cccgtaaaaa ataccgtctg aaacctgttc agacggcact
     3901 ttcaccctaa gcgttactcg gcggcatttt cctcacgggc aggcgcgtcc agcagggctt
     3961 tgatggacag acggacacgg cctctgtcgt ccacttccaa tgctttcacg ttcaccacct
     4021 gaccgacttg caggtagtcg ccgacattgc gtacgcgctc gtgggcgatt tggctgatgt
     4081 gtaccaaacc gtctttgccc ggcatcacgc tgacaatcgc gccgacattg ttatcgagga
     4141 ttttcaccac agtgccttcg tacactttgc ccacttccac ttcggcagta atctgctcga
     4201 tgcgtttttt cgccgcatcg ccggcttctt gagtggttgc ggcaatggta atcgtaccgt
     4261 cttcggcaat attgatttcc gtaccggttt cagcggtaat cgaacggatg gtttcaccgc
     4321 ccttaccgat aacttcgcgg attttgtctt ggttgatttt catcgtgaac aagcgtggcg
     4381 cgtgtgcgga cagctcttgc gggcccgcaa cggcggcttt catctgatcc aagatgtgca
     4441 gacgcgcttc tttggcctgt gccaaagcga tttgcataat ttctttggta atgccttgga
     4501 ttttgatgtc catttgcagc gcggtaacgc cttcggtcgt accggccact ttaaagtcca
     4561 tatcgcccaa gtggtcttcg tcgcccaaaa tgtcggtcag gacggcaaat ttgttgcctt
     4621 ccagaatcag acccatcgcg ataccggcaa cgtgtgcttt caaaggcacg ccggcagaca
     4681 gcaggctcag gcagccgccg cagacggaag ccatagagga agagccgttg gattcggtaa
     4741 tttcggagac cacgcgcatg gtgtagctga aatcttcagg tttcggcaat acggccaaca
     4801 atgcacgttt agccaaacgg ccgtgaccga tttcacggcg tttcggtgcg cccatgcggc
     4861 ccacttcgcc ggtagagtac ggcggaaagt tgtagtgcag cataaagcgg tcggtgtatt
     4921 cgccggacag cgcgtcgatg atttgctcgt cgcgcgaagt acccaaagtt gcaacggcca
     4981 aagcttgggt ttcgccacgg gtaaacaatg cagaaccgtg cgtgcgcggc aatacgctgg
     5041 tttggatgtt cagcggacgg acggtgcggg tgtcgcggcc gtcgatgcgc ggttggccat
     5101 ccaaaatttg gctgcggacg acatcggctt ccaagtgttt gaaaatgcct ttgatttcgt
     5161 tggctgccaa agtgtcggtt tcttcggtaa tcaaggcttc ttttaccgca ctccaagctt
     5221 cgtccaattt ggcagaacgc gcttgttttt gacggatttt gaacgcttct ttaatggttt
     5281 cgccggcaat cccgcggact ttggcaacca gttcctcatt ggtttcaggt gctttccaat
     5341 cccaaagttc cggattgact tcgtcggcaa attcattgat tgcattgatg gcaacctgca
     5401 tttgatcgtg gccgtaaacc accgcgccca gcatcacgtc ttcgggcagg attttggctt
     5461 cggattccac catcaacacg gcttttgaag taccggcgac caccaagtcc aattgcgatt
     5521 tcgccaattc ggctttagtc ggattcaaaa cgtacacgcc gtttacataa ccgacgcgtg
     5581 ccgcgccgat cgggccggca aacggtacgc cgctcaacac cagcgcggca gatgcaccca
     5641 acattgcagg aatatcagaa tcgatttcag gatcgacgga cacgaccatc gctacgattt
     5701 ggatgtcgtg gtagaaacct tcagggaaca gcggacgaat cggacggtcg atcagacggc
     5761 tggtcaggat ttctttttcg ctttgtttgc cttcgcgttt gaagaaaccg ccgggaattt
     5821 tgcctgcggc gtaagtgcgt tccaaataat cgacggtcag ggggaagaag tcttgacctt
     5881 ctttcacttc tttgttggtg gtaacggcaa ccaaaacaac ggtgtcgccc atagagactt
     5941 taacggcagc ggcggcttgg cgggcaattt cgccggtttc caaagtaacg gtctgattac
     6001 cgtattggaa ggtcttaacg tgtttgtcga acatcattgt tcctttcaaa ataccgcact
     6061 gctaaaacac taataatgca cactaaaatc cgaatgtgca tagttagggt ttcagaccgt
     6121 gcggcaggtt ataaacaagc tttcagacgg catttcggac gctgaaagca attgcggatt
     6181 ataaaggcaa ccatccttaa aatccagtat tgatacaata aaaaggccgt ctgaaccata
     6241 tttctttcag acggccttca aacttaaaaa tcaatcctga gtcagcaaag tcaatgcttc
     6301 ccgatacttc tcgacagttt tctgaatcac atcggcaggc actttcggcg caggggcttt
     6361 tttgttccaa ccgctttgtt ccagccagtc gcggacgaat tgtttgtcaa aagacggcgg
     6421 attggtgccg actttgtatt ggtcggcagg ccaaaaacgg ctcgaatcgg gagtcaatac
     6481 ctcatccatc agcgtcagcg taccgttttc atccaaaccg aattcaaatt tggtatcgca
     6541 aataataata ccgcgcgatt tggcatattc cgccgcttcg gtgtaaagcc gaaccgcctt
     6601 ggcgcgcact tcttccgcca attctttgcc gataatgcgt ccgcattctt caaagctgat
     6661 gttttcatcg tgatcgccga ctgcggcttt ggttgagggc gtaaaaatca cttcaggcag
     6721 ttgttgcgct tcctgcatac cttcaggcag ttgaataccg caaaccgagc cggttttttg
     6781 ataatctttc caaccgctgc ctgccagata accacgcaca atcgcctcta ctttcaccgg
     6841 agtgagcttt ttagccacga cggcgcgttt ctctaaagct ttggcttcgt tttcaggcaa
     6901 aacatcgtaa accgtttgac cggtaaagtg gttgggcata atatgcgcca gttttttaaa
     6961 ccaaaaattg gaaatctgcg tcagaatctc ccctttgctc ggaatcgggt cgtccaaaat
     7021 cacatcaaac gcggacaggc ggtcggaagc gaccatcagc atacgtttat cgtcgatttc
     7081 atataaatcg cgcacttttc caaaatagat ctttaccaaa ccaatctcac tcatttcgcc
     7141 ccccctgaaa atatcttgaa aataccgacc cgacacccga caggtttgaa tcacaaaccg
     7201 atattctagc cgaagtcggc gcaaaacaat acccatggca caaaaagcca acccgtcaac
     7261 cgtcggcaaa attttggcac tataataccg acagcaagtc ctacaataca cttttaccaa
     7321 aggaaatacc tcatgagaat cctattgaca ggctcgaaaa gccaactggc acgctgcctg
     7381 cgcgaccgtc ttccggaaga ctgggaaacc attgcgacgg attccgcatc cctagacatt
     7441 accgatgccg atgccgtctg caacatggtc aaaagtttcc aacccgacgc cattgtcaac
     7501 acggctgcct atactgccgt cgacaaggcg gaaggcgatg cggcagcggc atttgccgtc
     7561 aatgcttccg ccgtttacaa ccttgccttg gcagcacatc gcgcccatgc ccgattcatc
     7621 cacatctcaa ccgactatgt ctttgacggt aaagggaaaa gaccctatca ggaaagcgac
     7681 tttaccaatc cttccaatgt atacggacaa tccaaaaccg caggcgagct gctcgcactg
     7741 tctgccaatc ccgacagcct tatcctgcgg acttcttggc tgtttagcga atacggggac
     7801 aactttatcc gcacgatgct gaaccttgcg cgggaacgtt ccccgctgtc cgccgtccac
     7861 aaccaaatcg gctgcccgac ctatgccggc gacttgtccg ccaccatcat ccgcctgttg
     7921 cagcactcca atcccgttcg cggcatttac cactacgccg gcagcaaatc cgtatcctgg
     7981 tacgaatttg cccaacatat tttccaagcg gcatcgcaac agcagacatc cttccccgtt
     8041 cccgaattga ctgccgtttc agacaaggaa tatccgaccg ccgcccccag gcccgcatac
     8101 agcattttgg actgccgcaa aatcgaaaac gacttcggca tcaaaccgtc agactggcaa
     8161 aaagcccttg cacaggtcgt ttccaagctg ctctgatgcc gcccgcccct ctgtttccgc
     8221 cgtcaagcac cgccttggcg gttttc
//
LOCUS       DAWGRZ010000086         8217 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000086 DAWGRZ010000000
VERSION     DAWGRZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.086"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            284..1453
                     /gene="prpF"
                     /locus_tag="WHT44_001297"
     CDS             284..1453
                     /gene="prpF"
                     /locus_tag="WHT44_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ2533478.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFSIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            1867..3066
                     /locus_tag="WHT44_001298"
     CDS             1867..3066
                     /locus_tag="WHT44_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ2533479.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVGCGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            3425..4045
                     /locus_tag="WHT44_001299"
     CDS             3425..4045
                     /locus_tag="WHT44_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ2533480.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKVDNVQFRQLKAATPGPYTFILQ
                     ATKDVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRQGCGDTAVFGL"
     gene            complement(4172..4615)
                     /locus_tag="WHT44_001300"
     CDS             complement(4172..4615)
                     /locus_tag="WHT44_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ2533481.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            4789..5157
                     /locus_tag="WHT44_001301"
     CDS             4789..5157
                     /locus_tag="WHT44_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ2533482.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEETKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            5202..6356
                     /locus_tag="WHT44_001302"
     CDS             5202..6356
                     /locus_tag="WHT44_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ2533483.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVLAWLSAVFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(6413..7285)
                     /locus_tag="WHT44_001303"
     CDS             complement(6413..7285)
                     /locus_tag="WHT44_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ2533484.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDQASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHHCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVENLWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            7370..8182
                     /locus_tag="WHT44_001304"
     CDS             7370..8182
                     /locus_tag="WHT44_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ2533485.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSCEAGKVMNTLL
                     VYGRDGVRTGLYHKMHLFGFSGLGERYAEADTIRAGGDVPHLSAEGVPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGVVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT         1792 a         2232 c         2386 g         1807 t
ORIGIN      
        1 atttaatcca ctatactgta aatcgtcatt cccgcgcagg cgggaatcca gaaagtggaa
       61 ttgaggaaac ctttttatcc gatgagtttc tgtgcggata aatctggatt cccgcctgcg
      121 cgggaatgac ggggtttaat aatctgccgt atcacaacac agtagccgta gattggggcg
      181 aaccccgaca gtttgcggaa tcaaacggct ttggtcggag tggcagccta atccactata
      241 aaaatcgtgg gcagagccca cgctacataa ggagaatcta gaaatgccgc aaattaaaat
      301 tcccgccgtt tactaccgtg gcggtacatc aaaaggcgtg tttttcaaac gttccgacct
      361 gcccgaggcg gcgcgggaag cgggaagcgc acgcgacaaa atcctcttgc gcgtactcgg
      421 cagcccggat ccctacggca agcagataga cggtttgggc aacgccagct cgtccaccag
      481 caaggcggtg attttggaca agtccgaacg cgccgatcac gatgtcgatt accttttcgg
      541 gcaagtttcc atcgacaaac cttttgtcga ttggagcggc aactgcggca acctcaccgc
      601 tgccgtgggc gcattctcca tcgaacaggg cttggtcgat aaaggcaaga ttccttcaga
      661 cggcatctgc acggtcaaaa tctggcagaa aaacatcggc aaaaccatta ttgcccatgt
      721 accgatgcaa aacggcgcag ttttggaaac aggcgatttt gagctcgacg gcgtaacgtt
      781 cccggcagcc gaagtacaaa tcgaatttct tgatccagcc gacggcgaag gcagtatgtt
      841 cccaaccggc aatttggtcg atgaaattga tgtgccgaat ataggccgtt tgaaagccac
      901 gctcatcaac gcgggcattc cgaccgtttt cttgaatgcc gccgacttgg gctacacagg
      961 caaagagttg caagacgaca tcaacaacga tgccgcggct ttggaaaaat tcgagaaaat
     1021 ccgcgcttac ggtgcgctga aaatgggtct gatcagcgac gtatccgaag ctgccgctcg
     1081 cgcgcacacg ccgaaagtcg ccttcgtcgc gcccgccgcc gattacaccg cctccagtgg
     1141 caaaaccgtg aacgccgccg acatcgattt gctggtacgc gccctgagca tgggcaaact
     1201 gcaccacgcg atgatgggta ccgcctctgt tgccattgcg accgccgccg ccgtacccgg
     1261 tacgctggtc aaccttgccg caggcggcgg aacgcgtaaa gaagtgcgct tcgggcatcc
     1321 ttccggcaca ttgcgcgtcg gtgcagccgc cgaatgtcag gacggacaat ggacggccac
     1381 caaagcggtc atgagccgta gcgcacgcgt gatgatggaa ggttgggtca gggtgcctga
     1441 ggattgtttt taaattgacg tagcatgggt ttgcccgcga gccataaaaa ggtcgtctga
     1501 aaaacaagta aacatcaaat cactgaccat tcctttccct tgccctgtgg cggaaggcgg
     1561 caaatcacaa ggaagaacac ggaaaccccg ataaaagaca gcttcccgta ttaccgtcat
     1621 tcccgcgcag gcgggaatcc agacctgtca atatggagga ttggcagggg aaaacaggtt
     1681 tcgtgagttc tacattctgg attcccgcca cagcctgtcc tcgcgtaggc ggggacggaa
     1741 taacgataga aaatgcggca tacgctttgc ccaaagaggc cgtctgaaac accttgcgcc
     1801 tgatgtctgc ctttttcaga cgaccccaca ccaaaaaaac aaccacaaac tacaaggaga
     1861 aacatcatgt ccgaccaact catcctcgtt ctgaactgcg gcagttcatc gctcaaaggc
     1921 gccgttatcg accgaaaaag cggcagcgtc gtcctaagct gcctcggcga acgcctgacc
     1981 acgcccgaag ccgtcattac gttcaacaaa gacggcaaca aacgccaagt tcccctgagc
     2041 ggccgaaatt gccacgccgg cgcggtgggt atgcttttga acgaactgga aaaacacggt
     2101 ctgcacgacc gcatcaaagc catcggccac cgcatcgccc acggcggcga aaaatacagc
     2161 gagtctgttt tgatcgacca ggccgtaatg gacgaactca atgcctgcat tccgcttgcg
     2221 ccgctgcaca accccgccaa catcagcggc atccttgccg cacaggaaca tttccccggt
     2281 ctgcccaatg tcggcgtgat ggatacttcg ttccaccaaa ccatgccgga gcgtgcctac
     2341 acttatgccg tgccgcgcga gttgcgtaaa aaatacgctt tccgccgcta cggtttccac
     2401 ggcaccagta tgcgttacgt tgcccctgaa gccgcacgca tcttgggcaa acctctggaa
     2461 gacatccgca tgattattgc ccacttaggc aacggcgcat ccattaccgc catcaaaaac
     2521 ggcaaatccg tcgataccag tatgggtttc acgccgatcg aaggtttggt aatgggtaca
     2581 cgttgcggcg acatcgatcc gggcgtatac agctatctga cttcccacgc cgggatggat
     2641 gttgcccaag tggatgaaat gctgaacaaa aaatcaggtt tgctcggtat ttccgaactt
     2701 tccaacgact gccgcaccct cgaaatcgcc gccgacgaag gccacgaagg cgcgcgcctc
     2761 gccctcgaag tcatgaccta ccgcctcgcc aaatacatcg cttcgatggc tgtgggctgc
     2821 ggcggcgttg acgcactcgt gttcaccggc ggtatcggcg aaaactcgcg taatatccgt
     2881 gccaaaaccg tttcctatct tgatttcttg ggtctgcaca tcgacaccaa agccaatatg
     2941 gaaaaacgct acggcaattc gggcattatc agcccgaccg attcttctcc ggctgttttg
     3001 gttgtcccga ccaatgaaga actgatgatt gcctgcgaca ctgccgaact tgccggcatc
     3061 ttgtagccaa aaaagggacg agtccgcaaa aatgccgtct gaaaccccaa acgcccgatt
     3121 aggctgatga ggattttaga cggcattgtt catttttttg ttatcttgca tttttgtgcg
     3181 gacggtggaa tttcatcctg taaacataaa tatttgtcgg aaaacagaaa ccctccgccg
     3241 ccatttctac gaaagcagga aaccagcaac gcaaagcgac agggatttgt tggaaatgac
     3301 cgaaaccgaa cgaaccggat tcccgcctgc gcgggaatga cgggattttc tgtttttgtg
     3361 gaaatgacgg gattttgaat ttcgggcgta caatacggaa aacatgacga taaggaaaca
     3421 aaccatggca cagtttttcg ctattcatcc cgacaatccc caagaacgcc tcatcaagca
     3481 ggcggttgaa atcgtcaata aaggcggcgt ggtcgtttat ccgaccgatt cctgttatgc
     3541 cttgggctgc aaactcggcg ataaggcggc gatggaacgc atactctcca tccgcaaaat
     3601 cgatttgaaa caccacctga ccctgatgtg cgcagatttg agcgagttgg gcacatacgc
     3661 caaagtcgac aacgtacagt ttcgtcagct taaagccgcc acacccgggc cttatacttt
     3721 tattttacag gcgacgaagg atgtgccggc gcgcacgctg cacccgaaac gcaaaaccat
     3781 cgggctgcgt attcccgata atgccattgc acaagccctg ctgggggaat tgggcgagcc
     3841 gcttttaagc tgcaccctga tgctgcccga agacggcgaa ccattgaccg atccttatga
     3901 aatccgcgag cgtttggaac acgccgtcga tttggtgatt gacggcggct ggtgcggaac
     3961 cgagccgacc accgtcgtcg atatgaccga cggcacggaa ttggtgcgcc aaggttgcgg
     4021 cgatacggcg gtgttcggtt tgtagggaaa ccgatgccgt ctgaagcatc ggctgttcag
     4081 acggcattgc gcgccttgcc ggcggcagtc cgaaatgccg gcgcgtatcg cgctcggtcg
     4141 gaatatccgt ttgaaacggc attttgatgc attactgcac cgcaatcgga attctcggtt
     4201 cgtagagcag gtcgtaggtc ggcttgttga gcaggtcttg gagcgtgaaa ccgtccagat
     4261 acgtgaaaaa cgacttcatc gcgccgccga gtatgcccgt cagccggcag gacggtgtaa
     4321 tcaggcattc gttgttctcg cccatgcact cgaccagctg catcggttcg aggtggcgga
     4381 caaccgagcc gatgttgatg cggtcgggcg gtgcggcaag ccgcagaccg ccgccttttc
     4441 cgcgcacact gtggaggaag ccgcctttga ccagcgcggt aacgaccttc atcagatggc
     4501 ttttggaaat gccgtaggtt acggcgatgg tactgatgtt gaccagcgca tcgtcgttga
     4561 tggcagtgta gataaggacg cgcagcccgt agtccgtatg ttgtgtcaaa tacatgattt
     4621 tctcggtatg gattgttatt cttatcggta cggtttaagg ttcacggaca ataccttaat
     4681 ggttgaaacc ctgtccgtcg gggcggtaga atgcagcctg tctgcggcgg tatgccgtct
     4741 gaaacatccg cgctaccgtt tgagaatttg ttattgtaac tcaaaatcat gaaaccgttg
     4801 aaacgacatc ccgcccttat cgggctttcg cgtgaccacc accattcgct ttccctgtgc
     4861 gtgcgtctgt tgcggacgcc ggaagaaagg catcgggacg aactcgaacc gcatttttcc
     4921 gaattggaaa cccattttcg cgaagaagaa accaagtttg ccccaatttg gcagaatgtc
     4981 gcccccgaat tgaaacaacg tttcgagaaa gaccacgccc gactgcggca gatgatggca
     5041 agccccgaat acggtaacgc ggcgtggaat accgcttttg ccacaaccct gcgcgaccac
     5101 gcgcgctttg aagaacgcga gctgtttccc gccgccgaac cgtttttgcc ggcatgattc
     5161 cgttttgcgg taaatatatt aatgataaac aaggaacaca catgaaattt accaagcacc
     5221 ccgtctgggc aatggcgttc cgcccatttt attcgctggc ggctctgtac ggcgcattgt
     5281 ccgtattgct gtggggtttc ggctacacgg gaacgcacga gctgtccggt ttctattggc
     5341 acgcgcatga gatgatttgg ggttatgccg gactggtcgt catcgccttc ctgctgaccg
     5401 ccgtcgccac ttggacgggg cagccgccca cgcggggcgg cgttctggtc ggcttgacta
     5461 tcttttggct ggctgcgcgg attgccgcct ttatcccggg ttggggtgcg tcggcaagcg
     5521 gcatactcgg tacgctgttt ttctggtacg gcgcggtgtg catggctttg cccgttatcc
     5581 gttcgcagaa tcaacgcaac tatgttgccg tgttcgcgct gttcgtcttg ggcggcacgc
     5641 atgcggcgtt ccacgtccag ctgcacaacg gcaacctagg cggactcttg agcggattgc
     5701 agtcgggctt ggtgatggtg tcgggtttta tcggtctgat tggtacgcgg attatttcgt
     5761 tttttacgtc caaacgcttg aatgtgccgc agattcccag tccgaaatgg gtggcgcagg
     5821 cttcgctgtg gctgcccatg ctgactgcca tgctgatggc gcacggtgtg ttggcttggc
     5881 tgtctgccgt ttttgccttt gcggcaggtg tgatttttac cgtgcaggtg taccgctggt
     5941 ggtataaacc cgtgttgaaa gagccgatgc tgtggattct gtttgccggc tatctgttta
     6001 ccggattggg gctgattgcg gtcggcgcgt cttatttcaa acccgctttc ctcaatctgg
     6061 gtgtgcatct gatcggggtc ggcggtatcg gcgtgctgac tttgggcatg atggcgcgta
     6121 ccgcgcttgg tcatacgggc aatccgattt atccgccgcc caaagccgtt cccgttgcgt
     6181 tttggctgat gatggcggca accgccgtcc gtatggttgc cgtattttct tccggcactg
     6241 cctacacgca cagcatccgc acctcttcgg ttttgtttgc actcgcgctt ttggtgtatg
     6301 cgtggaagta tattccttgg ctgattcgtc cgcgttcgga cggcaggccc ggttgagaca
     6361 aaccgccgca gatttcgggt ctgggcggtt tgcttttcag acggcagggc ggtcagttgc
     6421 cgtccagcca gcggtcgcgt gtggttttgg cttcttcaaa atagcggtac agggcttcgc
     6481 ggtcgtcggt ggtcaggatg tttgccaaaa cgtccaactg tttgcccaag ccttgaacca
     6541 gttgcagcag gctgtctttg ttggcaaggc agatgtccgc ccacacggcg ggatgaccgg
     6601 aggcgatgcg ggtgaagtcc cgaaagcccg tggcggcgaa tttcagatat tcctgtccgt
     6661 cggggtggtc gagaatctgg tggacatagg cgaaggcggt caggtggggc atatgggaga
     6721 cggcggcgaa aaccgcgtcg tggcgttgcg cgtccatcgt ataaatttcc gcaccgaccg
     6781 cgtgccacag gttttctacc aaggcaatgc cgtctgaatg ttcgccgccg tgtggcgtga
     6841 tgatgagttt tctgtggcgg aacagcccga actgcgcggc ttgcgcaccg cttctgtccg
     6901 aaccggcaat tgggtgggcg gcgatgcagt ggtgcaggcg gtcgggcaga cagcggcgga
     6961 aggcttcgat gaccgaagat ttggtgctgc cgacatcgga aatccaagtg tgttccggca
     7021 aaacggggcg cagcgcggtc aaaatggcgg gaacggtggc gacgggcgtg gcaatcagta
     7081 ccaagtccgc accgccgatg ctgtccgcgt cgatggcaac ggaagcctgg tcaatcacgc
     7141 cgcgttccaa tgcacgttcg aggttgtcgc ggtcggtgtc gataccggta acggtgcgga
     7201 cgagtccctg ccttttgagg tcgagaacga acgaaccgcc gatcagccct acaccgatga
     7261 gggcaatatg gttcaaaatg ggcatttgtg taaacggttt tcgcaaagta ccgtcatggt
     7321 agcctatcgg cggaatatgc cgcaaggtcg gcaggaaaaa ggagaagaaa tggacaaaat
     7381 cagagttgcc gccgtgcaga tggtgtcggg cgtgtcgccg gaaaccaacg tcgccgccat
     7441 gaaacgcctg gtcgcacggg cggcggagca gggtgcggat tgggtgctgc tgcccgaata
     7501 ttgggtgctg atgggcgcaa acgataccga caaactcgcg cttgccgagc ctttgggcgg
     7561 cggacgcttt cagacggcat tgagcgaaac ggcgaaagaa tgcggcgtgg tgctgttcgg
     7621 cgggactgtg ccgctgcaaa gctgcgaggc gggtaaagtg atgaatacgc tgttggtgta
     7681 cggacgggac ggcgtaagga cggggctgta ccacaaaatg cacctcttcg gtttttccgg
     7741 tttgggcgaa cgctatgccg aagccgatac catccgcgcg ggcggggatg tgccgcactt
     7801 gtcggcagaa ggcgtgccgg tggcggcggg catttgttac gatgtccgct ttcccgaatt
     7861 tttccgacgc cagttgccgt ttgacgtatt gatgctgccc gctgcgttta cgcacacgac
     7921 gggcaaggcg cattgggagc tgctgctgcg cgcgcgtgcc gtcgaaaacc aatgttacgt
     7981 cgtggcggcg gcacagggcg gtttgcacga aaacggacgg cgcacgttcg gacacagcat
     8041 gattgtcgat ccgtggggcg acgtgttgga cgtattgccc gagggcgaag gcgttgttac
     8101 ggcagacatc gatgccaacc gcctgaacag cgtccgcaac cgcctgcccg ccttgaaata
     8161 ccgggttttg gatgccgtct gaaggttcag acggcatcgg tgccggggaa tcagaag
//
LOCUS       DAWGRZ010000087         8128 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000087 DAWGRZ010000000
VERSION     DAWGRZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8128
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.087"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            201..413
                     /locus_tag="WHT44_001305"
     CDS             201..413
                     /locus_tag="WHT44_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ2533486.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            561..2144
                     /locus_tag="WHT44_001306"
     CDS             561..2144
                     /locus_tag="WHT44_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ2533487.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESGAFSQTAI
                     NNLEARARLMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTIDESFGKFDYIIVHGIWSWVPDAVKDKIFSICWNNLT
                     KHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLDGGGKLPAIQKILNHNFYYIAHEYMEAFNDPIYVNGFIEWANRHRLAYIGDTNLH
                     VSFVSWMAEHTRERILALAGDDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETINFDENDILLSGIRDVMKTGEAFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTNYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2226..3323
                     /locus_tag="WHT44_001307"
     CDS             2226..3323
                     /locus_tag="WHT44_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ2533488.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFSWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            3437..4552
                     /locus_tag="WHT44_001308"
     CDS             3437..4552
                     /locus_tag="WHT44_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ2533489.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNALP
                     VCGQSLLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4677..5132
                     /locus_tag="WHT44_001309"
     CDS             4677..5132
                     /locus_tag="WHT44_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ2533490.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5203..5679
                     /locus_tag="WHT44_001310"
     CDS             5203..5679
                     /locus_tag="WHT44_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ2533491.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQQ"
     gene            5724..6017
                     /locus_tag="WHT44_001311"
     CDS             5724..6017
                     /locus_tag="WHT44_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEZ2533492.1"
                     /translation="MKPSLLLQSRKKEILAVFGKYPLICNPRVFGSVSRGDDTENSDI
                     DLLVDAKTGTTLLDLGGLQEELQKLLGIKVDLLTPDDISAHFRDKVLTEAVAL"
     gene            6014..6286
                     /locus_tag="WHT44_001312"
     CDS             6014..6286
                     /locus_tag="WHT44_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEZ2533493.1"
                     /translation="MKMQKELSVYLKQILQAAQYIRLYTDKMDYGQFSADTKTVQAVV
                     FNLFLIGENATHILKSYPEFAEETKYLNWIGMRGYCAIGLPMATLK"
     gene            6469..7386
                     /locus_tag="WHT44_001313"
     CDS             6469..7386
                     /locus_tag="WHT44_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ2533494.1"
                     /translation="MKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEFGEI
                     GIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAHAAS
                     VIDELKTKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLVASKTDLCT
                     PEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIQWLDIPVIEKSRYRLKALPDNTMGFQS
                     QGFTFPAGRDFDGEKLTNFFNDLPNMTEGLVRAKGVFQVLGTWVWLNWVDGQWGANQV
                     SWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            7451..8008
                     /locus_tag="WHT44_001314"
     CDS             7451..8008
                     /locus_tag="WHT44_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ2533495.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDDTVFHRVIDGFVIQGGGLTEDLAQKASDKAVANESGNGLKN
                     TAGTIAMARTTAPDSATSQFFINLADNASLDYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
BASE COUNT         2162 a         1946 c         2103 g         1917 t
ORIGIN      
        1 tgcgccgatt gccaaggcaa tgatgctcaa ccagggtttt tgcgaagcgt tcattgttgt
       61 tgtcctgatg ggaattgccg ccattatgcg caagcgtccg tattttttca aaccgccgca
      121 ccgtttccgc gtttgcccaa gttcacgccc cgacttacaa taacgcccgt cagaatgcag
      181 taccaacgaa aggaaaaatc atggaaaccc ttatcctcga catcggcggc atgagctgcg
      241 gcggctgcgt caaaagcgtt acccggatat tggaaggcgt aaaaggcgtg gcaagcgtcg
      301 aagtcagcct tgaaaacaaa agtgcgaccg tcggatacga ccccgcgcaa accgatgcag
      361 gggcgttgat tgaagccgtt gaagacggcg gctatgatgc cgcgttgaaa taaagcggca
      421 aaaatgccgt ctgaagcctt cgcgcctccc agatgccgtc tgaagtacgc tccgcgcctt
      481 cagatggcat ttgatttagt tgaacgggta ggatgaattg gttaaacttg cgggcaatag
      541 tgccttcaga caaacaaaat atgccgaaca agaagaaaaa agccgccttt gcgcgtgctg
      601 caacggagaa cggtgtgccg gacgtgtcca acatcaaaaa ttcatatgat gatttgatgt
      661 acgaatccgg cgcattctct caaaccgcca tcaataattt ggaggcgcgc gcccgcctga
      721 tgggtttgca gcccgcgccc gccgccaatg ccaaggtctt ggagctgggc tgttcgatgg
      781 gcggaaacat catcacgcag gcactttatt acccggatgc ggaatttgtc ggtatcgacc
      841 tgtccggcag gcaggttgca cagggcaacg ccatcattga aaaaatgggc ttgaaaaatg
      901 tgcgtctgga agaaaaagat attttgacca tcgatgagtc attcgggaag tttgactata
      961 tcatcgtcca cggcatttgg tcgtgggtgc ctgacgcagt taaagacaaa attttttcga
     1021 tttgctggaa caacctgacc aaacacggca ttgcctatat ttcatacaat gtttaccccg
     1081 gctggaaacg gcaggagcag ttgcgcgaaa ttatgtactt tgccggcagg gatgtgcttg
     1141 aagaaccctt ggaagcgcgg acgcggaaag gcttggacgc gctcaaggcg ctggcggaaa
     1201 ttttggaaaa cgacaagggc ttggacggcg gcggcaaact tccggcgatt caaaaaatat
     1261 tgaatcataa tttttattac atcgcacacg aatatatgga ggcgtttaac gacccgattt
     1321 acgtcaacgg cttcatcgaa tgggctaacc gccacaggct cgcatatatc ggggatacca
     1381 atttgcacgt gtcttttgtt tcttggatgg cggagcatac gcgggagcgg attctggcat
     1441 tggcagggga cgattatatt gccaaagaat tttacagtga tattttatcc gaccgccaat
     1501 tccgccgttc gcttttatgc cgtgaggaag tcggggatac tgtcaggcgt gatgagtcgg
     1561 ttgccgtcga agtgatagaa agtttgaatt tccgcccggc aagaggggaa acaatcaact
     1621 ttgatgaaaa cgatatcctg ctttcgggca tacgcgatgt gatgaaaacc ggagaggcgt
     1681 ttaaaacgga agatgttgcg gaaaatctcg cccgccgatt tcccggtttg gaatttgacc
     1741 gcatgaaaat caattcccag cttttattgc aaaccattct cgggcgtttt tctgtttcat
     1801 cagacaatgc gggcaaacca ttttttgaag accataaaac ctatgtgccg gcgcgcttta
     1861 caaactatgc cgccgccttt gtggaacacg gcgcggaagc gtttgtccgg cctgccaacc
     1921 gttacaacga aagcaccccc tcattcggat acgggcattt gtacattatg cgccaattgt
     1981 cgcggccgac gagcaaacag gcattgattg aaacggttgc cgaaaacttg aacatcgtca
     2041 gcaccacgcc cgacggtttg acattccatc cgcctgccga agtgtatgtg gaagaaatat
     2101 tggcagactt ggcagacagg cattttctcg tttcggcgga ttgacgggca agtcccggcg
     2161 gcaatccgtc cgatatattt tcagacagga aagacacaca ctgcgaaaag cggtgcacga
     2221 cacatatgaa ccagacattt accttgcccg atacgcgccc gtatccccaa aatccgatta
     2281 aaaaccacct gctgctcaat gcctaccagt tggcgcataa ttcttcccag gcttcgcgca
     2341 aactctcgtc cggccaactt caaaccgaaa tcaggggaat gcttgagcaa aaccactata
     2401 tcaacctttc cctcgcgctg acgatgtcgc ccgatgccgg aacttatgcc gcgctgcttt
     2461 ccagtgtgaa cgcggtactc gattgcgaga aagaaggcga agtgcagtgg ttcgccctgc
     2521 cggtcgtgct ggtgtccggc tgcaaaaaag aacgtgcaat cgagatgaag ctgccgacgg
     2581 aggcattgtt tgcctgcctg caaaactatc cgcacctgcg cgcgttgacg caagagacgc
     2641 aatggctgcc ttatcttgtg cattcttccg atttgagcgc ggtcgcgccg gatgagtggt
     2701 ggcgtgccaa acaaaatacc gaagcggcgg cgcaacactt gcgccgtttc gccccgcgcc
     2761 ctttgctgtt gcccgaaggg cagtccgtcc acgtcgttta cgcgctgggt ttcggcagcg
     2821 gcaaagttca gacggcattg ggtcagaacc ttcttcaggc aggtctgccc ctgatgcagg
     2881 tatggcagga aaatcttgca tcggaaggcg ttacgctgtt tgccaatccg ctttccccgg
     2941 attctccggt acgcgcgctt tcagacggca gccacacgcg ccaacgtatg gcgatggatg
     3001 tgtttgcggc aaacgcgata cgcgccgtcc gtatgcagag tccgcgcgtc ggcgtggtcg
     3061 ctgcggcaaa ggcgggcgga cagattttat tcgggtttaa tgcgaccgac ggcgcgtttg
     3121 aagtcgtgcc gcaggtgttt tcgtggcagc tttcctttac cgacaacatc gccgtcatcc
     3181 agcaaaattt cctcgacctg atggcggagt gccgcgtgga acacgtttac ctgttgcaca
     3241 atcccttggg cgaacaggaa agcatcccga gctatgcgga agcattgaaa cgggaagggc
     3301 acaatccgtt tttcagcgca tagtgatttg tagtagtgat aggctttctc atttatataa
     3361 taaatcaaaa taaagagcac aacacttttt cattctgtgt tgtgccttga gtgaaacgaa
     3421 aggatgaatt ataaatatga agattgtaat tgcaccggat tcgtttaagg aaagcttgac
     3481 agctcaacag gtagctgaag caataaaaag aggcttccaa caatcgatag cagatgtgga
     3541 atgtctcctc tgtcctgtcg gtgatggggg agaaggcact gtagatgcta tccgacattc
     3601 tcttgaccta gaagaaaaat gtctccaagt gacaggacct tttggacaaa aagaagtcat
     3661 gcgctatttt caaaaagaac aactagccct attcgaagtt gctgatttgg ttggtcttgg
     3721 aaaaatcccg ctagagaaac gaaatccatt acaaatccaa actcgtggta ttggagaatt
     3781 gattcgccac ctcattagtc aagaaattaa agaaatctat attggcgttg gcggtacggc
     3841 cagtaatgat ggaggtattg ggattgctgc tggtttaggt tatcaatttt atgatgagga
     3901 tggaaatgcc ttacccgttt gcggtcaatc cttactaaac ctagcttctg tttcaacaga
     3961 aaatcgctat gaaattcctg aagatgttca cattcgtatt ttagcagatg ttgtgagtcc
     4021 cttatgtgga caccaaggtg cgacctatac gtttggcaag caaaaagggt tggattctac
     4081 tatgtttgag gccgtagatc aggcaataca agatttttat gaaaaagtct cccctgcaac
     4141 attaaaactt aaaggagcag gagctggtgg aggcatcgct ggtggtttgt gcgcctttgc
     4201 tcaggcaagt atcgtatctg gaattgacac ctgcctggac ttgattgact ttgataagaa
     4261 agtgtcagat gttgacttgg ttatcgttgg tgaaggaaga ctagatcgtc aaagtttagc
     4321 agggaaagcg cctattggtg tagcaaaaag aacccctgtc ggagttcctg ttgtcgctat
     4381 ttgtggcagt ctcgttgaag atttgccttc cctgccattt gaaaatatcc aagctgcctt
     4441 ttctattttg gagaaaagtg aacctttaga agatagtttg aaaaatgcca gcctctattt
     4501 ggagcatacg gcttctaata ttggacactt attaaatatg cctaagattt agccaaacca
     4561 tttcttccag atggatgttt tggctggttc tgccttatgc ggttacgatt aaccgaccga
     4621 tgccgtctga aagggtttcc gcttcagacg gcatcgcgcc ggaacaggga ggggatatga
     4681 aaaaaccgaa aatccttttt gtctgcctcg gcaacatctg ccgttcgccg atggcggaat
     4741 acattttgcg ccgccgcgcc gccgaagcgg gcattcccct tgaagcggac agcgcgggga
     4801 catcgggctg gcacgacggc gaagatatgc accgcgagac ggcaaagata ttgaaaaaac
     4861 acggtatcga tgcttcaggc tttaccagcc gcaaaatccg ccaaagcgat gcggcggcgt
     4921 ttgactgcat catcgcgatg gacggcaaga atttgtccga attggaaaga accttcggca
     4981 ggcggccgga aaaaatattc aagctgaccg acctgatacc cgaaagcggt tacgaccatg
     5041 tccccgatcc gtggtatacg ggcgattttg aagaaacctt caggcttgcg gatgcgggct
     5101 gtcgggcatt gttggaaaag atttccaaat aaagcaattg aatacaaata taaaaccgcc
     5161 tcctgccgta tcggttgttc agacggcata aacagagaat ttatgaaaac aaatttcaaa
     5221 cagaaaatta tcgaacaggc acgcagcgaa ggcttgcagg taaccgcttt gcgcgagcag
     5281 gtattagata ttgtcttgca gcaaagcggc gtgattaaag cctacaacgt cttgtcgcag
     5341 atgcagcagc aaagcgaggg cgtgcttgca ccgcctaccg cctaccgcgc ccttgatttt
     5401 tgggcggagc agggcgtttt gcacaaagtg gcggcggtca acggctatat tttgtgcagc
     5461 cacgcgcagc acgagtgcga cgaccattgc cacgaccacg aagaagccga agcgcaccac
     5521 agcgcgttta ttttggtctg caccgaatgt ggcacggcgg acgagcaaac cctgagccac
     5581 gagtgggcgg cactgcgcgc aggcgttgcc gaaagcggct ttgcgctgaa agaagaacac
     5641 gttgttttaa ccgggatttg taaaaaatgt cagcagtaat cggtggtttg cattgataat
     5701 aagcctttcg aaaggagcag ataatgaaac cgtctctttt gttgcaaagc agaaagaaag
     5761 aaatattggc agttttcggg aaatatccat tgatttgcaa tccaagggta ttcggttcgg
     5821 tttctcgcgg agatgatacg gaaaacagcg acattgattt gttggtggat gcaaaaacag
     5881 ggacaacatt attggatttg ggcgggctgc aagaggaatt gcagaaactc ttgggcataa
     5941 aggtcgattt gctgacaccc gatgacatct cggcccactt tagagataag gtgttgactg
     6001 aggctgtcgc attatgaaaa tgcagaaaga attgtcggtc tatttgaaac agatattgca
     6061 ggcagcgcaa tatatccggc tatataccga caaaatggat tatgggcaat tttctgccga
     6121 cacaaagact gtgcaggcag tcgtttttaa tttgttcctt atcggtgaaa acgcaaccca
     6181 tatccttaaa tcgtatccgg aatttgccga ggaaaccaaa tatttgaatt ggatagggat
     6241 gagggggtat tgcgcaatag gattgcccat ggctactttg aaatgaacct gtctgtcgtt
     6301 tgggagacgg ttttgaacgt cataccggcg atgtcagccg atatatcgaa tttgctggaa
     6361 caattgtcaa ttgatgaaga acatgataac agctgccatc acttttaaca ttatttaatc
     6421 atttaaaaca ccctatcttc aaattttaaa gtgggacatc acatagaaat gaaaaaaacc
     6481 aaagtccacc tgatttcagg ttttctggga acaggcaaaa ccaccgcgct caaaagcctg
     6541 atggcgcaaa aagacccgaa cgaaaaatgg gtcatcatcg tcaacgagtt cggcgaaatc
     6601 ggcattgacg gcgcggtatt gagcgacaac ggcatccctg tggcagaaat cgccggcggc
     6661 tgtttgtgtt gcaccgccgg cccgcaaatg ggcgtaaccg tgcagaaaat gctgcgcgac
     6721 gccaagcccg accgcctgat gattgaagca agcggactgg cgcacgccgc cagtgtcatt
     6781 gatgaactga aaaccaaacc gctggacagc cttttggaaa tcggcgcagt ctttaccgtc
     6841 gtcgatccgc gccagttcat caaccccgat tacgcgcagc aggcattgta taaagaccaa
     6901 atcggcatct gcgacgtatt ggttgccagc aaaaccgatt tatgcacccc cgaacagctt
     6961 gccgaatttc acgacaaagc cgcaaaactg ttcccgccca aggctaaagt ggtcgaagtt
     7021 caaaacgcac aactcgatat ccaatggctt gacatccccg tcatcgaaaa atcacgctac
     7081 cgcctcaaag ccctgccgga caacacgatg ggcttccagt cgcaaggttt cacattcccc
     7141 gccggacgcg atttcgacgg tgaaaaattg accaacttct tcaatgattt gcccaatatg
     7201 accgaaggac tcgtccgtgc caaaggcgtg ttccaagtat tgggaacgtg ggtgtggctc
     7261 aactgggtgg acgggcaatg gggtgcgaac caagtgtctt ggcggcgcga ctcgcgtttt
     7321 gaattgattg ccaaatcgtt tgatgcggat ttaatcgaac aaaaactcaa agacgcattg
     7381 gaatagcgtt aacgagcctt cagacggcat aggaaatgaa aatgctgtct gaaggtctga
     7441 ggaggacggg atgaaaccca aattcaaaac cgttttaacc gcgctgcttt tggcggtttc
     7501 cctgccgtct atggcggcaa cccatgtttt gatggaaacc gatatgggca atatccgttt
     7561 ggttttggac gaatccaaag cccccaaaac cgttgctaat ttcgtgcgct atgcccgaaa
     7621 aggcttttac gacgacaccg tttttcaccg cgttatcgac ggttttgtta tccagggcgg
     7681 tggattgacc gaggacttgg cacaaaaggc aagcgataag gccgttgcca acgaatccgg
     7741 caacggcttg aaaaacaccg ccggcaccat cgccatggcg cggacgacag cccccgattc
     7801 cgccaccagc caattcttta tcaatctggc ggacaacgct tcgctcgact acaaaaacgg
     7861 acaatacggc tataccgttt tcggcagggt cgaaagcggc atgaacaccg tttccaaaat
     7921 cgcccgcgtc aaaaccgcca cgcgcggctt ttatcaaaac gtacccgtac agcccgtcaa
     7981 aatccgtcgc gttgttgtcg ggcagtaaca cgcagacaga cgttcagacg gcgtcgcccg
     8041 tttcccaaaa aacgccgttt aaagtaaaaa aatattttaa aacagacagt tgatattgac
     8101 aaattcaaac cgaggatttt aaaatgct
//
LOCUS       DAWGRZ010000088         8118 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000088 DAWGRZ010000000
VERSION     DAWGRZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8118
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.088"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(108..686)
                     /gene="grpE"
                     /locus_tag="WHT44_001315"
     CDS             complement(108..686)
                     /gene="grpE"
                     /locus_tag="WHT44_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ2533496.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            870..1688
                     /gene="cysE"
                     /locus_tag="WHT44_001316"
     CDS             870..1688
                     /gene="cysE"
                     /locus_tag="WHT44_001316"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ2533497.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
     gene            complement(2045..3556)
                     /gene="ubiB"
                     /locus_tag="WHT44_001317"
     CDS             complement(2045..3556)
                     /gene="ubiB"
                     /locus_tag="WHT44_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ2533498.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGEQVAVKVLRPNLLPVIEQ
                     DLSLMRFGAGWVERLFADGKRLKPREVVAEFDKYLHDELDLMREAANASQLGRNFQNS
                     DMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
     gene            complement(3591..4055)
                     /locus_tag="WHT44_001318"
     CDS             complement(3591..4055)
                     /locus_tag="WHT44_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ2533499.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLKENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPEPPSRNIKLLVFGLLIAFLGYLLGKIF"
     gene            complement(4052..4198)
                     /locus_tag="WHT44_001319"
     CDS             complement(4052..4198)
                     /locus_tag="WHT44_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533500.1"
                     /translation="MPSERSDGIFTFRKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            complement(4215..4553)
                     /gene="erpA"
                     /locus_tag="WHT44_001320"
     CDS             complement(4215..4553)
                     /gene="erpA"
                     /locus_tag="WHT44_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ2533501.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(4685..5371)
                     /locus_tag="WHT44_001321"
     CDS             complement(4685..5371)
                     /locus_tag="WHT44_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ2533502.1"
                     /translation="METFKDRLVFLWKSEARQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            5559..5831
                     /locus_tag="WHT44_001322"
     CDS             5559..5831
                     /locus_tag="WHT44_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533503.1"
                     /translation="MKKTGKYLIYTAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLSEQEMLAGIVYE"
     gene            6039..7967
                     /gene="dnaK"
                     /locus_tag="WHT44_001323"
     CDS             6039..7967
                     /gene="dnaK"
                     /locus_tag="WHT44_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ2533504.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDHIIAEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLITRSIEPCKIALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKAFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKTEALGAASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
BASE COUNT         2229 a         2248 c         1799 g         1842 t
ORIGIN      
        1 cgtctgaagc ccgacaggcg ggttcagacg gcattgccgc tttacttcga tcggtttata
       61 cgcaaaacgc gcttcaggaa ataaatcaga ttattcccca gacgccttca ggcttccttc
      121 tgcgccaccg taaccatagc cgggcgcaac acgcggtcgg acagcgtata acccttcttc
      181 atcacaccca ccacggtatt cggctcctgt tcgcttgcca ccgcctgcat cgcctgatgg
      241 atattcggat cgagcttatc gcccgcttta gggttgattt ccttgatttg cgtagcatca
      301 aatgctttct gcaactcgtt caaagtcatc tgcacgccca ttttcagcgc atcgaaattg
      361 ccgctctgat ccaaaagcgc catttccaga taatccttga ccggcagcat ttccacggca
      421 aacttctgtc cggcgaactt gtgcgtatcc gcaatttcct gctggtggcg gcggcgcagg
      481 ttttgctcgt ttgccaaagc gcgcagctgc tcgtctttca actgcgcttc cagctcggca
      541 atccgcgcct gcaaatcctc ataagccggc tctgcggcag cctgttcctg cacaccgtcc
      601 gcatttccta ctgtctcgac ggtttccacc gcctccacat tttcaaccgc ttcttcactg
      661 ttttgctgct gtgtctgttc gctcatatcg tatcccttaa aacaaattgg aaatcaaaat
      721 ccagttatta cccgcgcaag ataaggacat tttcaagaaa cttcaagcaa aagcagagaa
      781 tttcaaattc attttcaaat cccctaccga aaaaataata tagacggtaa gatactgccg
      841 accatatcga ttttcaaacg tcatcactca tgaaaaaaga ccatttaaac acaaccggtt
      901 tcgacctctg gcacaccatc cgcgaagaaa ccgcggccgc ggtctccgcc gaaccgatgc
      961 tggcaagttt tttgcaccaa accgtgttgc gccacgagtc cctcggctcc gtccttgcct
     1021 accacctttc cagcaaactc ggcagcccga ttatggacgt gcgcgcgctg tttgaaatct
     1081 accagcaggc gttgggcagc gacacccaaa tcggcaaatg cgtcgaagca gacttaaaag
     1141 ccatctacga acgcgatccc gcctgcgacg aatattcgct gccgctttta tatttcaaag
     1201 gcttccacgc gattcaggca caccgcatca accatcggct gtatctcgac gggcgcaaaa
     1261 cgctggcgta tttcctgcaa aaccgtatgt ccgaagtatt cggcgtggac atccatcccg
     1321 ccgcccgttt cggatacggg ctgatgctcg accacgccac cggctttgtt gccggcgaaa
     1381 ccgccgtgtt gggcaacaat atttcgattt tgcacggcgt aacgctcggc ggttcgggca
     1441 aagaaggcgg cgaccgccac cctaaaatcg gcgacggcgt gatgatcggc gcaaacgcct
     1501 cgatattggg caatatccgc atcggcagca atgccaaaat cggggcgggc agcgtcgtgg
     1561 tttcagacgt gccgccgtcc atcacggttg tcggcgtacc cgccaaaccc gtggcgcgat
     1621 cgctcaaaac accgtcggcg gatatggatc aaaatatcca gtttaccgaa atcgacttta
     1681 tgatttgaat atccgcacca atgccgtctg aaacgccaaa cgggcttcag acgacattgc
     1741 ccgcttcaag cctgcgcctt gatatgccgc cgatgtcggc acttgccagt cggacaaaat
     1801 gccttttatt caatacaccc gattcccacg caaccggaaa tatagtggat taacaaaaat
     1861 caggacaagg cgacgaagcc gcagacagta cggatagtac ggaatcgatt cacttggtgc
     1921 ttcagcacct tagagaatcg ttctctttga gctaaggcga gacaacgccg tactggtttt
     1981 tgttaatcca ctataaccga tttccttata aacgggcgga gtcgggcgat gattcggaca
     2041 aagcctattt caaaagcaaa atcagcagca aaacaaccgc aatcacagcc agccacaggc
     2101 tttgccgctg ctgcactttg accaaatgga tataggcatc acgcatttcc tgctggcggt
     2161 tttcatcaat caacgcactg attttgcgcg gcaatgaagg gatgatttgc gcccagtcgg
     2221 gggcttcgtt tttgaggttg cgccaaaggg ctttagggcc gacctgcccg ttcatccatt
     2281 tcaccaaaaa cggtttggcg gttttccaca agtccaaatc gggatcaagc tgccgtccca
     2341 agccttcgat gttgagcagc gttttttgca gcaataccag ctgcggctgg atttcgacat
     2401 tgaagcggcg gctgacttca aacaggcgca tcagcaccaa gccgaaggaa atctgcgaaa
     2461 tcggtttgtt gaacactggt tcgcacacgg cgcggacagc cgcttccaac tcttccgcgc
     2521 gcgtgtcggc gggcacccag cccgattcga tgtgggcggt ggcgacgcgc cggtaatcgc
     2581 ggttgaagaa ggcgaggaag ttgatggcga gataacgttt gtcgtaatcg gtcagcgtgc
     2641 cgacgatgcc gaaatcgagg gcgatgtagc ggttgtcggc ggcaaccaaa atattgccgg
     2701 ggtgcatatc cgcgtggaaa aagccgtcgc ggaagacttg cgtgaagaag atttccacgc
     2761 cgtaatcggc gagtttgtgc aaatcgatgc cgtctgcttt gagtttggcg atgtcggaaa
     2821 ccggcgtgcc gtccatccat tcgatggtca gcacgtcgct ggtgcagtag tcgtaaaaca
     2881 ccttcggcac aatcagcata tcgctgtttt ggaaattgcg tccgagctgg ctggcattgg
     2941 cggcttcgcg catcaagtcc aactcgtcgt gcagatattt gtcgaactcc gccaccactt
     3001 cgcgcggctt cagacgcttg ccgtcggcaa acagacgctc gacccagcct gcaccaaagc
     3061 gcatcagcga caaatcctgt tcgatcacgg gcaaaaggtt ggggcgcaaa actttaaccg
     3121 ccacttgttc gcccgaatgc aggcgggctt tgtgtacttg ggcgatggac gcgctggcga
     3181 tgggctcggt ttcaaattcc gcatacagct tttcgatgga ctgacccaac gatttttcga
     3241 tttgttcacg cgaaagccgc gcgtcaaaag gcggcacttt gtcttgcagc tttgccagtt
     3301 cgacggcgta atcgtgcgga atcaaatcgg ggcgtgtgga caaaacctgc ccgaacttga
     3361 tgaaaatcgg ccccaggctt tccaaggcaa ggcgcagacg gacagcaggc ggttcgtttt
     3421 tcaatttgga cgactgcggc atcattttca gcagagcgca tatccaaccg ctacccatca
     3481 gcgaagcaca cagacctgcc agccgatagc ggtaaaaagt cccgacaata accgtcaggc
     3541 gtttcaacca tttcatatat aaaatcccga atacagcatt tatcggacaa tcaaaaaatc
     3601 ttaccgagca gatagcccaa aaaggcaatc agcagcccga aaaccagcag tttgatattg
     3661 cgggacggcg gttcgggttg cgaaggtgcg gaaatatcat cttcccgccc gccgtgaagt
     3721 tcaagatatt cgggcatttc cccatccgcg ccttctcctt cgccctcccc ttcctccggc
     3781 ggcacgaccg tttccaactc catatatctc cgggtaataa tatccaaacc caaatagctg
     3841 aaataatcat gcaactcttc ccgtttcgcc caagtgcatt tttcctcaac cagcacttga
     3901 ggtttggaat cgagcgattc gatcaatcct tccgcctttt cctcgccgag attctcctta
     3961 aggcactccc ggataagccc gcgatccact tggggcgggt aagaaacata tacatcgtac
     4021 aaatcgtttc ggtaatcttt actcatctca atcataaggg aacaaatacg cctgataccg
     4081 ttccgcccgt tcctttcggg aaacccgatt catcttcaaa caatcggatt atatgtgaat
     4141 tcatcccgat aatatatttt ctaaaagtaa aaatgccgtc tgaacgttca gacggcatca
     4201 cacaaaccaa gcggtcatac ggaaaacgac gatccgcaac cgcaggttgt ttccgcattc
     4261 ggattgcgga tgacgaattg cgaaccctgc aaactttccg tatagtcgat ttccgcaccg
     4321 accagatatt gatagctcat cggatcgacc aaaaagacca aaccgttttt ctcaatttca
     4381 aaatcgtcgt cgtttttgat ttcgtcaaaa gtaaatccgt actggaaacc cgaacagccg
     4441 ccgccattga caaaaacccg caatttcaaa tcgggattgt tttcttcggc aatcaaatcg
     4501 gcaactttgg cacagcagct gtcagtaaaa ataatagggc tttcatccga catggtgtta
     4561 ttccttaata taatattttc tgcattctct ctcaaacccc cttcaaaggc aagcaggctt
     4621 cgcctttgaa aaaggaattg ttccaataga taagtttgac cgcctgcaat ttcaagcccc
     4681 aaactcaaat cgtcctgccg aaccactcga cacgcccgat aatctccaca tcatcggtca
     4741 aatcgttcaa attgatttca aaagcaggat aagcctcgtt tgcagaaatc acattgataa
     4801 tcccgcccgg tacaatctgt aaacgtttaa ccagcagatt ttcattaatc cgcaacacat
     4861 acagaccgtc cctcggcgta ttttcaccat gattgaccaa aatcgaatcg ccgtcattca
     4921 aaaccccctc catcgaatcc cccttgacgg aaattacaga caggtttttc gtatcgcggg
     4981 taacataatt ctcaatccaa tgccgtctga acgccattgt aaataccggt tcctcatgat
     5041 cgacaaactg cccgtatccc gcagccgccc gaatatcata tctcggcacg aagacaaact
     5101 cgtccgtatc gacttcattg cccaaagtat cgtaagcaag ggattttttt ggggcttcat
     5161 ccggaaacgg attaccctcc ccggtcagca gccaatcgat actacacccc ttcaactgct
     5221 tgattttttt caatgtttca gactttggca gaccgccttc attccatatc ctgctgaagc
     5281 ccgcaatcgt catttcaata tcggatgcga tttttgcctg cctcgcttcg cttttccata
     5341 aaaaaaccag tctgtcttta aaggtttcca taaatatcct tccactcccc ttcattcaat
     5401 catctaaagt aaacatgaaa tatattttac cttagctatc caaaaaacgg aacaattatg
     5461 ataaactaaa tttaaattat gatttttctt acttaataca taatttttca gatttttcca
     5521 taggtttaat cctgaattga cagaaaaagg atagaaaaat gaagaaaacc ggcaaatatc
     5581 ttatctatac tgcggcattt accgcattct gctttgcctt ccaagaaaac cgttctgaag
     5641 ccaaacagcc cgacatcact ttatccgcat ccctgtgcga acaattcaac atactgaacg
     5701 ccaaagatat ggatgcagaa caagtctccc tttccaaaga atgcgacatc atcgagtctt
     5761 cacacgactg ggaaaaagag tacggcaact tgagcgaaca ggaaatgctc gccggcatcg
     5821 tctatgaata aacctgcctg ccatttgaaa cattatgctt gaatgcattg gagccaaatg
     5881 tattaaatca aatataaaac caatatattc ataaagttat atacttatag ccatgctgta
     5941 gcttgaaaca gcccgccgcc cgccctattt acagcccatc gggacaaaat gttccaacat
     6001 atttctcaaa ataagcaaaa tcaaatagga ttatccacat ggcaaaagta atcggtatcg
     6061 acttaggtac aaccaactct tgtttggcca tttccgaaaa cggtcaaacc aaagtgatcg
     6121 aaaacgcaga aggcgcacgc accacgccgt ccgttatcgc ttatttggac ggcggcgaaa
     6181 tcctcgtcgg tgcgcctgcc aaacgccaag cggtaaccaa cgccaaaaac actatttacg
     6241 ccgccaaacg tttgatcggc cacaaatttg aagacaaaga agtccaacgc gacatcgaat
     6301 ctatgccttt cgaaatcatc aaagccaaca acggcgacgc atgggtaaaa gcacaaggca
     6361 aagagctgtc tcctcctcaa atttccgcag aagtcctgcg taaaatgaaa gaagccgccg
     6421 aagcttactt gggcgaaaaa gtaaccgaag ccgtgattac cgtccctgcc tacttcaacg
     6481 acagccaacg tcaagccacc aaagacgcag gccgtatcgc cggtttggac gtgaaacgca
     6541 tcatcaacga gccgaccgca gccgctttgg cattcggtat ggacaaaggc gacaacaaag
     6601 accgcaaagt agccgtatat gacttgggcg gcggtacttt cgatatttcc atcatcgaaa
     6661 tcgccaacct cgacggcgac aaacaattcg aagtattggc aaccaacggc gataccttct
     6721 tgggcggtga agacttcgac caacgcctca tcgaccacat catcgccgag ttcaaaaaag
     6781 aacaaggcat tgatttgaaa caagacgtga tggctctaca acgcctgaaa gaagctgccg
     6841 aaaaagccaa aatcgaattg tccagcggcc agcaaaccga aattaacctg ccgtacatca
     6901 ccatggacgc aaccggcccg aaacacttgg cgatgaaaat tacccgcgcc aaattcgaaa
     6961 gcctggttga agacctgatt acccgctcta tcgaaccttg caaaattgca ttgaaagatg
     7021 ccggcttgag caccggcgac atcgacgacg taatcttggt cggcgggcag tcccgtatgc
     7081 cgaaagtaca agaagccgtt aaagccttct tcggcaaaga accgcgcaaa gacgtgaacc
     7141 ctgacgaagc cgttgccgta ggcgcagcga tccaaggcga agtattgagc ggcggccgca
     7201 gcgacgtatt gctactggac gtaactcctc tgtctttggg tatcgaaacc atgggcggcg
     7261 tgatgaccaa actgattcag aagaacacca ccatcccgac caaagcgtcg caagtgttct
     7321 ctaccgccga agacaaccaa agcgcagtaa ccatccacgt actgcaaggc gaacgcgaac
     7381 gcgcttctgc caacaaatct ttgggtcagt tcaacttggg cgacatcgca cctgcaccgc
     7441 gcggtatgcc gcaaatcgaa gtaaccttcg acatcgacgc caacggcatc ctgcacgttt
     7501 ccgccaaaga caaaggcacc ggtaaagcag ccaacatcac catccaaggt tcttcaggtt
     7561 tgagcgaaga agaaatcgaa cgcatggtga aagatgccga agccaatgcc gaggaagata
     7621 aaaaactgac tgaattggtc gcttcccgca accaagccga agccctgatt cactctgtga
     7681 aaaaatcttt ggccgactac ggcgacaaac tcgacgcagc cgagaaagaa aaaatcgaag
     7741 ccgcgctgaa agaagccgaa gaagcagtta aaggcgacga caaagccgct atcgatgcca
     7801 aaaccgaggc gctgggcgca gccagccaaa aactggggga aatggtttac gctcaagcac
     7861 aagctgaagc ccaagcaggc gaaagcgaac aagccaatgc ttctgcaaag aaagacgatg
     7921 atgtcgtaga tgccgacttt gaagaagtaa aagacgacaa aaaataatta ataccgtctg
     7981 aaaaaagcgc gaaccgtttg attcgcgctt ttttcaattg agataaaaga ccatagcata
     8041 acagaggttc caagttcatc taccgtaata ttcccaaacc caagttccaa ctgttgtatc
     8101 ggttctctgg aaattttt
//
LOCUS       DAWGRZ010000089         8042 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000089 DAWGRZ010000000
VERSION     DAWGRZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.089"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(68..814)
                     /locus_tag="WHT44_001324"
     CDS             complement(68..814)
                     /locus_tag="WHT44_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ2533505.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(993..1664)
                     /locus_tag="WHT44_001325"
     CDS             complement(993..1664)
                     /locus_tag="WHT44_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ2533506.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WHT44_001326"
     CDS             complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WHT44_001326"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ2533507.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSGELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFICIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2481..2963)
                     /locus_tag="WHT44_001327"
     CDS             complement(2481..2963)
                     /locus_tag="WHT44_001327"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ2533508.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(2995..3684)
                     /locus_tag="WHT44_001328"
     CDS             complement(2995..3684)
                     /locus_tag="WHT44_001328"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ2533509.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAIPIADTLKCADGGNISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGVRPLLIQGDVRNLKLTQPQD
                     AYIVRLLLDAV"
     gene            complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WHT44_001329"
     CDS             complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WHT44_001329"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ2533510.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVVHTETKRSGKLKVIRADENELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            complement(4571..5881)
                     /locus_tag="WHT44_001330"
     CDS             complement(4571..5881)
                     /locus_tag="WHT44_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ2533511.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="WHT44_001331"
     CDS             complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="WHT44_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ2533512.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6056..6355)
                     /locus_tag="WHT44_001332"
     CDS             complement(6056..6355)
                     /locus_tag="WHT44_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533513.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6706..7902
                     /locus_tag="WHT44_001333"
     CDS             6706..7902
                     /locus_tag="WHT44_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ2533514.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
BASE COUNT         1900 a         2405 c         1965 g         1772 t
ORIGIN      
        1 ataaatttgc cacaaaaaag ctgcctcaaa tgaatacccg ggcagctttt tgttgatatg
       61 actccaatca gcggtgttgc ggattgtaac gtttttccaa acgcaggaat atccagccta
      121 agaaagtcgt catcaacaga taaatcaggg cgacggtgta aagcggttct tcataaaccg
      181 aataccggcc cgtaatcgta ttctgaacat acgccaactc cgccacagca atgaccgaca
      241 gcagcgagct gtctttcaag agcgtgatga actcgctcgc caaaggcggc agcatgcggc
      301 gcaatgcctg cggcagaatc acatagcgca tcgcctgcgg ataggtcagc cccaaagaac
      361 gcgccgcctc catctgtcct ttgtctatag actggatgcc cgcgcggaaa atctcacaga
      421 tatacgcccc cgagttggcg atcagtgcca aagaaccggc aatcagcggc ccgtatccgc
      481 gacgcagcgc gattgccgcc tcgccgctga ccaaaatgcc gtctgaagga tggacgaaaa
      541 acggaaacca cacatacgcc caaatcacaa tctgcacaaa cagcggcgta ccccggaaca
      601 gcgtaacata cagcagcgaa actttacgca acgcccacgc cagcacgcgc atcggcgcac
      661 cggctttttc caagtgaatc aggcgcgcca acgccaacaa cagacccaat accgaaccgc
      721 ccgccgttgc cacgaccgtc agccccaagg tcgtcagtgc gccgtaaaga aacatccagc
      781 ggtattcgta aataatgtca aaacgaaaat ccataaaccg tccgtatcaa aaaccggcgg
      841 aactgccgcc gttgcaaaat aatccgccat tttaccgtaa aaaccgccgc ctgaactttt
      901 ttatcgcggc agacggcggt tgcgcgtctc cgcaaaaatg cagggcgcgc ggttttcaga
      961 cggcatttgc cgttcaaagc cgtgcggtgt ctttaccaaa tgcccaacca ttcgcccacg
     1021 gcatccatcc aatccttatt gcccccgccg ctcctgcctg ctcggcggta cgcccacggc
     1081 gcttgcggat ttttagcttt ccacaatcct ttgcgttccc tttccgcctg aatttgagcg
     1141 tcggcataat cggcaaaatc cgccttatcc tgctgttctt tagcataact tttataatgc
     1201 cacgccgccc cgtcctgcac ctgcatcagg ttcaaatcgg ttttgccgac agaaacctgc
     1261 gccacttcgc gctggtagcg gtcggtatcg aacacgcgca cgctgacttt cctgccttcc
     1321 gccgccgcgc gcaggttgtc gcgcgaacgc gtgccgtaag cctgtttcat ctccggcgcg
     1381 tcgatatacg ccatccggat tttgtgtttc gcgccgtcgc cgtcgataac gtgaagggtg
     1441 tcgccgtcat agactttgga caccgtgcct gtgtagcggt ggccggattt cgccgatgct
     1501 cggcggcggg cgggcgcgtc ggaacccgcg tcccctgccg cgccgagtac gtcgagtacg
     1561 gcaaccgccg tccgcaccgc ctcgctgccg taccccgtat aacccaacgc acccaaaagc
     1621 gacagggcga cgggaagcca tttcatgatt tttttaatct gcatattttt caaatgccga
     1681 tgccgtctga acatatcgga atcggatttc agacggcatc ttaacgtcag gattaccctt
     1741 ggcagggata gatgactttc gcaccctctt ccgtccccaa aatcaacaca tcggcggcat
     1801 cgcgggcgaa tatgccgttt tcgagcacgc cggtgatttt gttgatttcg tcttccatcg
     1861 tcagcggctg atcgatattc aagccgtgga catcgacgat ttggttgccg taaaacgtgg
     1921 tgtagccgat acgcagttcg ggctgtccgc ccatagcgag cagtttgcgc gaaacaagag
     1981 agcgcgcgct ttcgacgact tccacaggca gagggaattt gcccaaacgt gaaacatatt
     2041 tgctttcatc cgcaatgcag atgaattttt cggacgcgct ggcgacgatt ttttcgttga
     2101 ggtgcgcgcc gccaccgcct ttaatcattt gcagggcgtg gttcacttca tccgcaccgt
     2161 cgatatagac cgccaacccc gatacttcgt tcaaagaaac gacgggaata tcgtactggg
     2221 caagcagttc gccggatttt ttggaagtag ataccgcgcc tttgattttt ttgccgctct
     2281 tacccaaggc ttcgatgaaa aagttgatgg tcgagccggt accgatgccg atatattcat
     2341 tttcgggtac gaattcgact gctttttcgg cggcgatgcg cttgagttcg tcttgtgtcg
     2401 tcatattttt gtcctttggg aaaccgtatc aacaaacagc cgccatctta acattttttt
     2461 gcacgtcctg cccgccgcgt tcaaatgcgt accagcaata ccgccgcctg cgcctctatg
     2521 ccttccatcc gcccgagata gccgagtttt tcgttggttt tgcctttgat gttgacgcac
     2581 gaaatgtcta tgcccaaatc ggcggcgatg ttggcacgca tttgcggaat gtgcggcgcg
     2641 agtttgggtt tctgtgcaat cacggtcgta tcgacattga ccgcctgcca accctgcgcc
     2701 tgaacgcttt gatacgccgc acgcaaaagg acgcggctgt ccgcatcttt gaactctgcg
     2761 gcggtgtcgg ggaaatggct gccgatatcg cccaaacctg ccgcaccgag cagcgcgtcg
     2821 gtaacggcgt gcagcagcgc atcggcatcg gagtgtccga gcagcccttt ttcaaatggg
     2881 atttcaactc cgccaagtat cagctttctg ccttcggtca gttggtggac atcgtagccc
     2941 tgtccgatac ggatgttcgt catcgtttgt gttcctgatg ttttgaattg aagttcagac
     3001 ggcatcgagc agcagcctga cgatgtatgc gtcctgcggc tgcgtcagtt tcaaattgcg
     3061 cacgtcgccc tgtatcagta gcggacgcac acccaatttt tccacggcgg acgcttcatc
     3121 ggtaatgccg tccaagtttt ccgcagccaa tgcgcggtgc agcagcccgg cgcggaaaag
     3181 ctgcggcgtt tgcgcctgcc aaaggctcgt ccgctcgacg gttgcactaa tgttcccacc
     3241 gtccgcgcac ttgagcgtat cggcaatggg aattgccaaa atcccgcctt cggcggcgtt
     3301 gcccgcctgt tctatcaacc gcgtcaaagc ttcagacggc aggcagcaac gcgcggcatc
     3361 gtgtaccaga atattgtcgg tttccgccgc caaaccggtt tccaacagtt ttgccacacc
     3421 gttgcggacg gtttcggcgc gggtctgtcc gccgtttttc cacacccgaa cctgtggaaa
     3481 tgccgtctga accttatcgg caaacgtgtc ttcgggcgag acgacaacga cggtcaaatc
     3541 gacggcctca tgccgttcaa aaatcccaat cgtatgttct aaaacggttt tgcttccgat
     3601 ttcgacatat tgcttgggtt tgtccgcacc gaaacgcgcc ccgatgccgg cggcgggaat
     3661 cagcgcgata tttttgcgct tcatgcgtcc gtcccgccgt tttcagacgg cacggcttcc
     3721 ttgcgccaga tacaggcttc gcccaagccg tccaaatatt gcccgtgcgc cgccaactcg
     3781 ttttcgtccg ccctgatgac tttcagtttg ccgctgcgtt tggtttcggt atgcaccacg
     3841 ggtttggttt ccattttttc ctctgcggcc gcacccatca ggtcgaactg ccgccgcgtc
     3901 atagcaagat agacttcgcc caaaagttcg cagtcgatca atgcgccgtg caggacgcgc
     3961 ttgctgcggt cgacggaaaa acggttgcac aaggcatcca ggctggcttt ctgcccgggg
     4021 aacatttcgc gcgccatcgc cagggtatcg gtaacggtac agccgagttc ctcaacggtc
     4081 ggcaacccca tccggcggaa ctccatattg aggaagccca cgtcgaattt ggcattgtgg
     4141 ataatcagtt ccgcaccgcg caggaaatcg gcaatctgcc tgccgacctc tgcaaacggc
     4201 ggcgcgtttt tcccttccaa aacctgtatc gtcaagccgt ggacgcgtgc cgcctcttcg
     4261 ggcatatcgc gctcggggtg gacatagagg tgcaggtttt tgtcggtcat ttggcggttg
     4321 accatttcca aaccggcaaa ctcgaccaag cggtcgccgc cgtcggcata cagaccggtg
     4381 gtttcggtat cgaggatgat ttggcgtgtc gtcatatcgg tgtctttctt ctatcttcgt
     4441 aaattgctta ttttttaagc aatgtatttt tctgttttca tttcaatgca caaacccact
     4501 tattcacagt gtgttcacaa cattgggcag gcggattgtg tattttgggg acaatttttt
     4561 cagacggcat tcaaggtttt ttcctgattg ccgccgcgcc taaaaaccgc ctttcgcgct
     4621 taatcaaaaa taccgacaac ggaatattgc ccaaagcgac aatcagatac aacaaggaaa
     4681 tgctgtcaaa caaaaacagc aacaccgcgc tcaaaacggc agcggaaacc ataaaaatac
     4741 cgttaacgat attgttggcg gcaacggcgc gggcgcggaa agtctcgcta ctggcggttt
     4801 gcagccaggt atagagcgga acggagaaaa atccgccgaa aaagccgatc agcgtcatca
     4861 ccgccatcac gggatatgcc catccttgcg ataaaaacca aaaaatgccg ttcagccctt
     4921 caaaacggtg tccgtgcgtc agccacacca aaaccaagcc gcaaaccgtc aaacccaacg
     4981 caccaaccgt tacccaagcc aacatcaggc gttccctgct gaacttggca cacagtaccg
     5041 aaccggcggc aataccgatg gaaaacagag caagcatcag gttgaaaaca ttgtcgttgc
     5101 cgcccagatg gatttgggta aaggtcggca gttgcgtggt ataaaccgcg ccgacaaacc
     5161 aaaaccacga aataccgata atggcggtaa aaacgggctt gtgccgcacc gtttcacgca
     5221 gcagggattt tgtgccacgg acaatattcc actcaatttg tgtatcggca gccttggcgg
     5281 gtacggacgg cataaacagg ctgccgaccg tgcctccgac ggcgaccagc aaaaccagta
     5341 tcccgacaat ataaggcggt acacctgcca ccgccgttcc caaaatctga ccgaacagga
     5401 tggcgacaaa cgtacccgat tcaatcaggc tgttgcccat catcaactct ttgtcgtcga
     5461 gataatcggg caggatggcg tatttcagcg gcccgaacag cgtcgattgc gcgcccatgc
     5521 aaaacagaca cgccaaaagc agcggggcag accggatata aaacccgtat gccgccaccg
     5581 ccataatgat catttccagc accttgaccc aacgcgccaa aacggccttg tcgaatttgt
     5641 tacccaactg ccccgacagc gaggaaaaca ggaaatacgg caaaataaac agcaacgcgc
     5701 ccaagttcaa catctgtccg gcaggcagga agccgttttg ccccaaaccg taaaacccaa
     5761 tcatcacaaa cagcgcggtt ttgaacacat tgtcgttgaa cgcgccgaga aactgcgtag
     5821 cgaaaagagg tgcgaaacgg cggcttttaa ccagtcccaa accgcctttt ttagcgtaca
     5881 tcgttttccc tctctttttc aatcagttta cttgtcgaat catcatccat caggatgcgg
     5941 tgcgccggcc cttccaagtc gtcaaactgc ccgtttttgc ccgaccacca aaaaaaccag
     6001 ccgatgacaa acgccaaaat aatgctgatg ggcaccaata taaacatgct ttccatcaca
     6061 tattccctgt caaatcgttc aaaacaaaag tctgccccga cacggtcaga tattcgttac
     6121 gcaaagttcc gacgggagct tcgtcaaaaa acagctcgat acggtctttg accacgcgcc
     6181 aatattgggg gatttccgtc tgaccgaacg gcgacaggac atgattttcc attccgcctt
     6241 caagtttgac ggcaaaacgc ccgctttgcg gccgtgcttc cgattcgtcg tcggcaagca
     6301 ggatgaaaaa gcctatatgc cgtcccgatt ggtcatgaat actgaaataa tgcataaatt
     6361 tcccacccgc cttttttcag acgacaccaa ctaaaaacag ggcgaatgta ccagtttgga
     6421 cgggaagaat gcaaagaaat tctccctccc ccagccgaaa acaccggcaa accgcatatc
     6481 cccctttttt ccgtcaaaat gcctgacttc cgccattttc acgcaaacgc ccgattaagc
     6541 caagcaattg caaagatttt ttgctagaat agcctgcttc ttttatcaac cttttcagac
     6601 ggccccacta ctttcccgcc caggaaggca aaacggattc ggcacgaatc cggttagtat
     6661 ccgtgtccga ttccaatgcc gtctgaaact ttccggagta agaaaatgtc ccaaaaattg
     6721 atcttggttt tgaactgcgg cagctcgtcc ctcaaaggcg cggtcctgga taacggcagc
     6781 ggcgaagtcc tgctcagctg ccttgccgaa aaactcaacc tgcccgatgc ctacatcaca
     6841 ttcaaagtaa acggcgaaaa acacaaagtc gatctgtccg cacatcccga ccacaccggc
     6901 gcggtcgaag ccctgatgga agaactcaaa gcccacggcc tcgacagccg catcggcgcc
     6961 atcggccacc gcgtcgtcag cggcggcgaa ctgtacagcg aatccatcct cgttgacgac
     7021 gaagtcattg ccggcatcga aaaatgcatc ccgctcgccc ccctgcacaa ccccgcccac
     7081 ctcttgggcc tgcgtgccgc gcaaagcatt ttcaaaggcc tgcccaacgt cgtcgtattc
     7141 gatacctcct tccaccaaac catgcccgaa gtcgcctaca aatacgccgt tccgcaggag
     7201 ttgtatgaaa aatacggcct gcgccgttac ggcgcgcacg gtaccagcta ccgcttcgtc
     7261 gccgacgaaa ccgcgcgctt cctcggcaaa gacaaaaaag acctgcgtat ggtcattgcc
     7321 cacttgggca acggcgcgtc cattaccgcc gtcgccaacg gcgaatcgcg cgacaccagt
     7381 atgggcctga ccccgctgga agggctggta atgggtacgc gcagcggcga catcgatcct
     7441 tccgtattcg gcttcctcgc cgaaaacgcc aatatgacca tcgcccaaat cactgaaatg
     7501 ctgaacaaaa aatccggtct gctcggcatt tccggcctgt ccaacgactg ccgcaccatt
     7561 gaagaagaag ccgccaaggg gcataaaggc gcgaaattgg ccttggatat gtttatctac
     7621 cgccttgcca aatacatcgg cagtatggcg gttgccgcag gcggtttgga cgcactggtc
     7681 tttaccggcg gcatcggcga aaactccgac atcatccgcg aacgcgtgat cggctacttg
     7741 ggcttcctcg gtctgaacat cgaccaagaa gccaacctga aagcccgctt cggcaacgcc
     7801 ggcgtgatta ccactgccga cagcaaagcc gttgccgtgg tcattccgac caacgaagag
     7861 ctgatgattg cccacgacac tgcccgtttg agcggtctgt aaggttttat ccgcacacga
     7921 actgcctccg gaaatggagg cagttttttt atccggcttt ccatgcttaa acagcactgc
     7981 ctcttttcag acattgacgg ttgcagccgc ttacctgaac cttatagtgg attaaattta
     8041 aa
//
LOCUS       DAWGRZ010000090         7906 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000090 DAWGRZ010000000
VERSION     DAWGRZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.090"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(51..2093)
                     /gene="recG"
                     /locus_tag="WHT44_001334"
     CDS             complement(51..2093)
                     /gene="recG"
                     /locus_tag="WHT44_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ2533515.1"
                     /translation="MMSPETRKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(2179..2622)
                     /gene="secB"
                     /locus_tag="WHT44_001335"
     CDS             complement(2179..2622)
                     /gene="secB"
                     /locus_tag="WHT44_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ2533516.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVEMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDADLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(2643..2900)
                     /gene="grxC"
                     /locus_tag="WHT44_001336"
     CDS             complement(2643..2900)
                     /gene="grxC"
                     /locus_tag="WHT44_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ2533517.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            3113..4606
                     /gene="rng"
                     /locus_tag="WHT44_001337"
     CDS             3113..4606
                     /gene="rng"
                     /locus_tag="WHT44_001337"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ2533518.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(4641..5891)
                     /locus_tag="WHT44_001338"
     CDS             complement(4641..5891)
                     /locus_tag="WHT44_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ2533519.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLQADDDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALPLQAER
                     LNNMPLPPDAARQPAVLQQSIRRNKHLLEQLLALARSQSDEIPLTKTTFGLQSRFRQV
                     LQELMPLALEKCQDIGVAVGGDVEVSADETEIYTLIKTFADNAVRYTPPEGRIDLGFT
                     DEGKYLAVWVEDNGKGIPESERARVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGYL
                     ELSDSRRFGRGLLIRALLDKETLK"
     gene            complement(5887..6062)
                     /locus_tag="WHT44_001339"
                     /pseudo
     CDS             complement(5887..6062)
                     /locus_tag="WHT44_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6285..7598)
                     /locus_tag="WHT44_001340"
     CDS             complement(6285..7598)
                     /locus_tag="WHT44_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ2533520.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
BASE COUNT         1858 a         2341 c         2058 g         1649 t
ORIGIN      
        1 aaatcagatt tccgttacac ttttttccaa tatttcagac ggcattttgc tcacacgccc
       61 aaataccctt ccctgccgga aagccacctt gccaaatgcg cttcgacgat ttcggggttt
      121 tgttcaatca gcatcggggc ggtttcgcgc gcttgttcca agaggtgcag gtcttcttcg
      181 agcttggcga aacgcagcat aggcacgccg ctttggcgcg cgccgagaaa ttcgccgggg
      241 ccgcggatgt tgaggtcttg gcgggcgatt tcaaagccgt cggtgtgttc gtagatgact
      301 ttcagccgcg ctttggcgag ttcgcccaag ggttcggcaa acaggaggac gcacacgctt
      361 tctgccgcgc cgcgcccgac ccgaccgcgt aattggtgca gctgcgccaa gcccatgcgc
      421 tcggcgtgtt cgatgaccat cagggcggca ttgggcacat ctacgccgac ttcgatgacg
      481 gtggtggcga ccaagacgtt cagccccccc gaagaaaacc gcgccatcac ttcggccttt
      541 tcggcggcct tcatgcgccc gtgtaccagt ccgatattga gttcgggcaa tgccgtctga
      601 agccgggcga gggtttcggc ggcggtttgc agttgcaggg tttcgctttc ttcaatcaat
      661 gggcagaccc aatacgcctg ccgccctttt cggcaagtgc cgaggacgaa gccttcgact
      721 tcggcgcggc ggacgttgtt gacgaggcgc gttttaatcg gtgtgcgccc gggcggcaat
      781 tcgtcgatga cggacacgtc caaatcggcg aaaaaactca tcgcaagcgt gcgcgggatg
      841 ggcgtggcgg acatcatcag ctgatggact tcgcgccctt tgtttttgag ggcgaggcgt
      901 tgggcaacgc cgaaacggtg ctgttcgtcc acaatggtca agcccaaatt gtgaaacgcc
      961 acgccgtctg aaaacagggc gtgcgtgccg acggcgattt tgacgctgcc gtcggcgagt
     1021 ttggctttgg cttcgtcttt ggctttttta cgcaaactgc caaaaaggcg gacaacttca
     1081 atgcccaaag gttcgagcca ttgtttaaat ttaataaaat gttgttcggc aaggatttca
     1141 gtgggcgcca ttacagccac ctgcgcaccg gattcgatag ccgtcaaagc agacaaagca
     1201 gccacaatgg ttttgccgct gccgacatcg ccctgcagca ggcggtgcat cgggtaggtt
     1261 tgcgccatat cgcggcagat ttcggaaaca actttttctt gcgcatcggt cagggcaaac
     1321 ggcagggctt ggcgcagggc ttgggtcaat gtgccgtcgc cgcccaatgc cgccgccgtg
     1381 ccgccgatac gcttctgtcg cgccaagcgc atcgaaagct gttgcgccaa aagttcatcg
     1441 aatttgagcc gttgccatgc aggcagcgtg ccgtctgaaa gctgatgaat cgtgaaactc
     1501 ggcggcggcg aatgcaaaag acgcaggctt tcggcgaggt gtggcagctt cagacggcac
     1561 agcagggcat cgggcagcgt gtcgtgcagc ggcgtaacgt ccaacgccgt ctgaataata
     1621 cggcgcaaag tgggctggtt caaaccgttt acggtcgggt aaaccggcgt gaggctttcc
     1681 gccaaaccgc cgccctcggc atcgcggatt ttgggatgaa tcatctcgtc gccgtaaaag
     1741 ccgtgtttga tttcgcccac ggcgcggatg cgtttgccga ccgccgtctg tttctgatgg
     1801 ctggcgtaaa agtggatgaa gcgcagaaaa aggacgctgc cggagccgtc ggcgatttgg
     1861 acaatcagct gcttgcgcgg tttgaacgtt acttcctgat ggataacctc cccctcgacc
     1921 tgacacggca cgccaatcgg cgcgtcctta atcggcataa tgtgcgtctc gtcctcgtaa
     1981 cgcagcggca ggtgcaacac caaatcccac gcggtatgga ggttgagttt gtcgagcttc
     2041 ttggcggaaa catcggtgat tttgagctgt tttcgggttt cgggcgacat cataggcaga
     2101 ttcctttgga cgcgcctatt ttatccgaaa acaaaaatgc cgtctgaaac ggattcagac
     2161 ggcatcgaca ggcaggaatc aagccccggc ggcttcggct tcctgctgtt gttggtaaat
     2221 cgcctcaaaa ttaatcggcg cgagcaggac gggcgggaag ccggcgcgcg ttaccgtacc
     2281 ggaaacggct tcgcgcgcgt aagggaagag gatgttcgga cacgccacgc cgagcagcag
     2341 gtcggcatct tcttcgggga tgttttccaa acggaaaata ccgctttggg ttacttcgtt
     2401 caaaaacatc gtgcgctcgt tatccaattt ggcggttacg gtaacggtta catccacgtt
     2461 gtagtagccg tcttccagct tttggctgcc ggtggaaacg cgcatctcca cttcgggctc
     2521 gccctgttcc aaaaagattt gcggcgcgtg cggcacttcc aaagacaagt ctttgacata
     2581 cagtcgctcg atgctgaata cgggttgcag ttcttcgctc attttgtttt cctagttggg
     2641 ggttaagggt tcagcagtcc gtccagcccg ccttcctgct ggaggcggta gaggtcggta
     2701 aatccgccga cgtgcgtttc gccgatgaaa atctgcggca cgctgcgctg tcccgaaagc
     2761 tgctgcattt cggcaaaggc ttcggggctt gcatcgacac ggatttcgtc gatatgtccg
     2821 acacctgccg cgtgcagcag ccttttcgcc atcgcgcagt aggggcaaaa cggacctgtg
     2881 tacatggtaa cggtctgcat attgggtttc cgaaagtttt gcaatgataa tcaatatagg
     2941 ggcatttccc ctgtttggca agtgcggaac agatgcacgt tcaaacggca tgtgcggaat
     3001 gtgtcaaagt ttctttttta aagtatgata gacattgtga aaaatatttt tgcacccgcg
     3061 ctgcgcggcg gaacggatgc aaaatatttt tattacattt tcaggaaaaa ccatgttgtc
     3121 aggactcccc atccccaaag acatcgcgcg cccgcccgaa acgatattgg tcaacatcac
     3181 gccgcaggaa acgcgcgtag cggtgttgga ggaaaacaat atctgcgagc tgcacatcga
     3241 gcgcaacagc gaacacagcc tagtcggcaa tatctatctg ggcgtggtgc gccgcgtgct
     3301 gcctgggatg cagagcgcgt ttatcgacat cggcttggaa cgcgcggcgt ttttacacat
     3361 cgtcgatgtc ctcgaacaac gccgcaaccc cgaagaaacc cagcgcatcg aacatatgct
     3421 gtttgaaggg cagtctgttt tggtgcaggt catcaaagac ccgatcaaca ccaaaggcgc
     3481 gcggctttcc acccaaatct cgctggcggg gcgtttcctc gtccatcttc cgcaagaaga
     3541 ccacatcggc gtgtcccaac gcatcgaaga cgatgccgaa cgcagcagcc tgcgcgaacg
     3601 cctcgacaag ctcctgccgg aaaatgcctg ccggggctac atcatccgca ccaacgccga
     3661 aaacgccacc gacgaacagc tccagtccga catcgactac ctgaccaaag tgtgggaaca
     3721 catccaagaa caggcgaaaa tccggccgcc cgaaaccctg ctttatcagg atttgccttt
     3781 aagcctgcgc gtgttgcgcg atatggtcgg ctgcgacacg caaaaaatcc tcgtcgattc
     3841 caccgtaaac cacgggcgca tgacgcgttt tgccgaacaa tacgtccacg gcgcattggg
     3901 caggatagag ctgttcaaag gcgaacgccc gctgtttgaa acccacaacg tcgaacagga
     3961 aatcagccgc gccctgcaac cgcgcgtcaa cctcaacttc ggcagctacc tgattatcga
     4021 atccaccgaa gccatgacca cgatagacgt gaacaccggc ggcttcgtcg gcgcacgcaa
     4081 cttcgacgaa accatcttcc gcaccaacct cgaagcctgc cacaccatcg cccgcgaatt
     4141 gaggctacgc aacctcggcg gcatcatcat catcgacttc atcgatatgg cacaggaaag
     4201 ccaccgcgaa gccgtgttgc aggagcttgc caaagccctc gccttcgacc gtacccgcgt
     4261 taccctgcac ggttttacca gcctagggct ggtcgagctg acgcgcaaac gctcgcgcga
     4321 aaacttaaac caagtcctct gcgaaccctg cccttcctgc caaggcagag gccgtctgaa
     4381 aacgccgcaa accgtatgct acgaaatcca gcgcgaaatc gtccgcgaag cgcgccgtta
     4441 cgatgccgaa agtttccgca tcctcgccgc ccccaacgtc atcgatttgt ttttggacga
     4501 agaatcgcaa tccttggcaa tgctgataga tttcatcggc aaaccgattt ctctggcggt
     4561 cgaaaccgct tacacgcagg aacaatacga catcgttttg atgtaaaaaa tgccgtctga
     4621 agccttcaga cggcatctgt ctatttcagg gtttccttgt ccaacaacgc gcgtatcagc
     4681 agaccgcgtc cgaaacgtcg gctgtcggac aattccaaat atccgccgta ttttttggca
     4741 agcgtgtcgg cgatggacag acccagcccc gtcccctgct gctccgttcc caaaatacgg
     4801 taaaacggat cgaggacacg ggcgcgttcg gattcgggaa tgcctttccc gttatcttcc
     4861 acccacacgg caagatattt cccttcgtcc gtgaaaccca aatctatcct gccttcgggc
     4921 ggcgtataac gtaccgcgtt gtcggcaaag gttttaatca gcgtatagat ttccgtttcg
     4981 tcggcagaca cttcgacatc gcctccgacc gccacgccga tgtcctgaca tttttccaaa
     5041 gccagcggca tcagttcctg caacacttgg cggaaacggc tttgcagacc gaatgtcgtt
     5101 ttcgtcagag ggatttcatc cgactgcgaa cgcgccaatg ccaaaagctg ttcgagcagg
     5161 tgtttgttac gccgtatgct ttgctgcaaa acggcaggct gccgcgccgc atcgggtggg
     5221 agcggcatat tgttgagccg ttccgcctga aggggaaggg cggtcatcgg cgtacgcaat
     5281 tcgtgtgccg cgtcggcgac aaaccgctga cggtggcgga tgtcttcatc cgcacgtttc
     5341 aaaagcaggt tgatggcggt tacgaaacct ctgatttcac tgggaatatt gtccacactc
     5401 aaagcagaca ggtcattgat tcggcgttgt tcgagacttt gcgacaattt gcggacgggg
     5461 cgcatggctt tgtgcgtaat ccacacggtc agcaaaatca tcagcggcag tgccgccaac
     5521 aggggcaaca cgctttgccg tgccgcatcc gccgccaaat cttcacggta ttcgttttcc
     5581 tgcataacgg caatccgtcc ctgctcggtc gtgcggatat agacgcggta ataatcgtcg
     5641 tcatcgtccg cctgaagcgt gtgcagaccg tccgccagat gcgcaggcag gctgacaaca
     5701 gggtcttcct gctgcggcat ctgtaccaaa atacgcgtat cgccgtcgcc ctcgggcaaa
     5761 gtttcgggtt tggaatcggg ggcgacgtac aatgccgcct gacggagcag gtcgtcctgc
     5821 aacgcttccg tttcgtggaa ggtttcgtag taggaaaaca tacctgcaag cattgccagc
     5881 ggaacaaaca tccaaaccgt cccgccccgg caaacccaaa tccagcagca tcaagtcata
     5941 aggctgggca gcggcagccg cgcgccgttt ttgacccaat ccaccgcata gccgccgtct
     6001 ttcaaacttg ccgacaccgc ctccgcaatc atcgcatcgt cttccaccag caaaacacgc
     6061 atcaactttc ccttcaaaat aaaccgtgcc tattctaaca ccccaaaatt agccgcaatt
     6121 tagcggtctt tacgcttgcc ggtatttttc aaaactgcag cacaaaaaaa ccgcgccggc
     6181 aactgccttc agacggcatt ggggcgcgat tgcaacacac gggcaggcag gcagagcctg
     6241 cgacagacca caggaacgat tcaggcttca gacggcttcg ccgtttacgg cagaggcacg
     6301 attcctgccg ctatcgaact ggccaatatc gccagcgaca aaccccacgc ccagaaaaac
     6361 gaataacgga tgtgtttgcc catcgacaat ttcgccaaac ccaagcccat ccacaaagcc
     6421 ggcgaaagcg gcgtaacaaa agtgccgacg atactgccga tcaacatcgc ataacctgct
     6481 gcttcgggcg ccacgcccgc ctgcgaggta atctgctcca caatcggaaa cagtccgaaa
     6541 taataagcgt ccgtactcaa aaccaactca agcggaatgc ccaacacacc gatggcaata
     6601 tgcagataag gcagcagcgc gtccggcagg atatgcacaa tgtctttgga aatcgcgtcc
     6661 aacatccccg cacccttcaa aatccccaaa aacgtacctg ccgccaaaat aatggacgcc
     6721 atcatcaccg cgccgccggc gtgggcataa atccgctcca tctgttcctg cgggctgcgg
     6781 taattcaaaa gcaacgccgc cgttgcagcc agcataaata cataacccgg tgggaagatg
     6841 cccgaaaaaa gcaggctcat cgccgccaaa aacagcagga cattccacca aaacagtttc
     6901 ggacgcgcca atttttgttc ttcttccgac aaaggcaccg gctttatcaa atccgccacg
     6961 gcgggcaacg cgcccaactc ccggacaatc cgcctttttt cacgcacacc caaaagcagg
     7021 gacagcgcaa ggataaacac cacaccgata atttgcaccg tcaacaaagg tttatacaat
     7081 tcgcccacat ctgcgcccaa cacgcttgca acccgcccgg tcggcccgcc ccacggcaga
     7141 aggttaatca atcccgcact ggaagtcagc agcaaaaaca gcaggtaagg attcatatgc
     7201 agacgcttgt aaagcggcaa aagggcgggg acgaccaata aaaacgtcgt cgcacccgcc
     7261 ccgtccaact gcgccaccac cgacaccaag accgtcccca cactcactgc cacgatatta
     7321 ccccgagtca gcttaatcaa accgcctatc atcggacgga acagccccac atcgttcatg
     7381 attccaaaaa acaaaatgga aaacataaac ataatcacaa tctgcatcac cgatttggtg
     7441 ccgcccgaat aaaattcttt taattgggat acatcaaacc ccgccagcaa cgccccaaac
     7501 agcggcacca agattaatgc gatgatgggc gacacttttt ccgtcagcag cagccatacg
     7561 atgaccccga taatcagcag tccgataaac gtcagcatca tttctccttt attttatttt
     7621 aaacagaaaa ccgaccgtgc aggcaaaacc gcccacagac gcgggataag ccctgcattc
     7681 tactttttta ttttgaaaca agtcaatcgg tcatttcctc ccatttacgc ctgccgccat
     7741 tcctgcatcc gtccgtcatt tcacagcggc aaccgatacg gaacaaccgg taaatcggta
     7801 tcgggacggc gcgggggcat tcatcccggt gcgccgattc aaacgaaacc gcccctatca
     7861 ttgcggagcg cggggcgtgc cgtacacgcg ggattttata gtggat
//
LOCUS       DAWGRZ010000091         7874 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000091 DAWGRZ010000000
VERSION     DAWGRZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.091"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(60..135)
                     /locus_tag="WHT44_001341"
     tRNA            complement(60..135)
                     /locus_tag="WHT44_001341"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(99..101),aa:Glu,seq:ttc)
     gene            complement(855..2267)
                     /gene="trkA"
                     /locus_tag="WHT44_001342"
     CDS             complement(855..2267)
                     /gene="trkA"
                     /locus_tag="WHT44_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ2533521.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(2365..3888)
                     /locus_tag="WHT44_001343"
     CDS             complement(2365..3888)
                     /locus_tag="WHT44_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ2533522.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMNVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPRKWQAKIGIIPVES"
     gene            complement(4195..5001)
                     /locus_tag="WHT44_001344"
     CDS             complement(4195..5001)
                     /locus_tag="WHT44_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ2533523.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDTAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKVSSSEDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPAKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            complement(5053..5304)
                     /locus_tag="WHT44_001345"
     CDS             complement(5053..5304)
                     /locus_tag="WHT44_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533524.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            5404..6165
                     /locus_tag="WHT44_001346"
     CDS             5404..6165
                     /locus_tag="WHT44_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533525.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRARVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRIRFDTKKDSVEFETGQIESGENADEAESGTNEPAVSENGENDAEETLMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            6310..7479
                     /gene="metZ"
                     /locus_tag="WHT44_001347"
     CDS             6310..7479
                     /gene="metZ"
                     /locus_tag="WHT44_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ2533526.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSAGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVAKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELLAQVAMYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLSDHPQAALIRKQQNGGGIVIGFEVADQEAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            7566..7772
                     /locus_tag="WHT44_001348"
     CDS             7566..7772
                     /locus_tag="WHT44_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ2533527.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT         1712 a         1928 c         2292 g         1942 t
ORIGIN      
        1 aattgcggga atgacgactc ataataaaaa acctgcttga aacaagcagg tttttgaatt
       61 ggcacgccca cggggattcg aaccccggtt gccgccgtga aagggcagtg tcctaggcct
      121 ctagacgatg ggcgcgtgga aacggacatt atacatactt atagaaattt agcaatacga
      181 tgccgtctaa acaaatgtca ggggggcgtt tgtctgatcg catcacaata taaaaacata
      241 taaaaaacat tggaatatca agcaattcat ttttttgcgt ggtaatgccg acaacggaag
      301 gcagctttat tttgaggcgc aaaggtcttc gatgcacacg atgcgctaga cttggcaggt
      361 caatatgggc gtgcgcagta tgttctgatg cggcggattc gcctgaaaac aagccggaac
      421 ggtcagatgc gttccggatt gtttttttgt cgggggggtg ccgctatatc aaaacaggcg
      481 ggcgatgccg tctgaaactt cgtcattccc gcgaaagcgg gaatctagaa cttttaaatt
      541 ttcagacggc ttttgaatat tgccgttgcc cgacgttctg gattcccgca acttttcgtc
      601 attcccgcga aagcgggaat ctagaacctc taaattttca gataaccttt gaatattgcc
      661 gctatcccaa ggtctggatt cccgcctgcg cgggaatgac gggtctttta taacctttga
      721 atattgccgc tatcccaagg tctggatgcc cgcttacgcg ggaatgacgg ggagggcgga
      781 tgccgtctga agcccaccta ccattatttc cgcttccata ccgcttggcg gcaatatgtc
      841 cggaatgggg cggtttatcc gaaaaagccc attttgacct ggatgagttt ttccagttcg
      901 ttcaggatgc gccggcgcga gacgaaaaag atgatgtggt cgccgtcttg gatgacggtt
      961 tcggtatggt gtcccataat ggtttcgccg gttccggcgc ggacgacggc ggcaatgtgg
     1021 cagccttcgg gccatttgat gccgctgatg cgcctgccga tgatggcgga agtttttttg
     1081 tcgccgtgtg cgacgacttc gatggcttcc gccgtgccgc gccggatggg gtggacggca
     1141 acgatgtcgc cgcgccggat gtgggcgagt atcgagccga tggtgatgag gtggggggag
     1201 acgacgatgt cgattttgtt gccttcgagc aaatcgacgt agcttgagcg gttgacgatg
     1261 ccgatgacgc gcttcgcgcc gaggtttttc gccaaaaggg cggacataat gttgctttcg
     1321 tcgtcgttgg tcagggcgca gaatacgtcg atttcgtcga tgtattcgtt gtcgagcagg
     1381 gtttcgtcgg ttgccgaacc ttgcaggacg agggtgttgt cgaggttttc ggctatccat
     1441 tcggcacggc gcggccggca ttcgatgatt ttgacgttgt atgcgtgttc gagctgcttg
     1501 gcgagacggt agccgatgtt gccgccgccg gcaatcatga tgcggcgggt gctggtttct
     1561 ttggggcgca attcgggtat gaccgcgccg atgttttcgg cggcggcggc aaataggatt
     1621 tcgtcgcctt cgatgatgac ggtttgcggc gcggggacga tgaggcggtt gttgcggtaa
     1681 acggcgcaga tttggcagtc ggccccgtcg ggcaaatctt gggcgatgtc ggcaatgctg
     1741 cgtccgacaa gcagtccgcc gcgccgcgcc tgtatgatga ccatccgcac gcggtcgtct
     1801 gcaaaacgta aaacctgcaa tgcgcccggg cagtctatca ggccggcaag ctgttcggta
     1861 acgagctgtt cggggctgat ggtttcggtt atgccgaata tggaaaggct gccgttttcg
     1921 ttgttttcga gcttggggct gaggtattcg aggtattcgc tggaacggac gcgcgcgatg
     1981 cggccgggga tgttgaacag gtcggcggca actttgcagg cgacgatgtt ggtttcgtcg
     2041 ctgcgggaga gcgcgagcag caagtcggca tcttccgcgc cggcgcgttc taatgtgaag
     2101 ggggatgcgc cgttgccgaa aacggtttgg acatcgaggc ggctgcctgt ttcctgcaat
     2161 gctttttcgt cgatgtcgat aacggttacg tcgttgttgg gtatggcggc aaggttttgt
     2221 gcgacggtag aacctacctg tccgttgcct aaaatgagga ttttcacggt gtgggtcgcc
     2281 gagtgatgaa atggtggatt gtaccgcaac cgggggcgga aatgccgtct gaaggcagat
     2341 tttgtgttca gacggcatgg cgcctcaaga ttcgacgggg atgatgccga ttttcgcctg
     2401 ccatttgcgc ggggcggtgg cgtggatgga ttcgcctttg ctatctacgg cgacggttac
     2461 gggcatgtct ttgacttcaa attcgtaaat ggcttccatg cccaattcgg ggaacgccaa
     2521 gactttggaa gatttgatgg cttttgccac gagatacgcc gcgccgccga ctgccatgag
     2581 gtacacggct ttgttgtcgg cgatggcttc gcaggtggcc acgccgcgct cggatttgcc
     2641 gatcatgccc aagaggtcgg tttgttcgag catttggcgg gtgaatttgt ccatgcgggt
     2701 ggctgtggtc ggacctgccg gaccgacgac ttcatcgccg accggatcga cggggccgac
     2761 gtagtaaatc aggcggttgg tgaaatcgac gggcaattct tcgcctttgt tgagcatatc
     2821 gacgaggcgt ttgtgtgcgg catcgcggcc ggtgaggatt ttgccgttca acagcaatac
     2881 gtcgccggtt ttccagcttg ccacttcttc tttggtcagc ttgtcgacat cgacgcgttt
     2941 gccgttgtcg gggctgtaag tcaaatcggg ccagtcttcg acgcgcggcg gcgtgagttc
     3001 gacagggcct gagccgtcca attcaaattc gacgtggcgg gtggcggcgc agttcggaat
     3061 catggcaatc ggtttggagg cggcgtgggt cggataatcg aggattttca cgtccaacac
     3121 ggtggtcagt ccgcccaagc cttgtgcgcc gatgcccagc gcgttgactt tttcaaagag
     3181 ttcgaggcgc agggcttcgg tggtggacaa ttccgcgccg gacgcggcct tttcctgcaa
     3241 ttcttgaatg tcgatgtggc tcatcaggga ctcttttgcc atcagcacgg ctttttcggg
     3301 cgtgccgccg atgccgatac ccaagatgcc gggaggacac cagcccgcgc ccatggtcgg
     3361 gatggttttc aatacccaat cgacgatgtt gtcggaagga ttgagcatgg cgagtttgga
     3421 tttgttttca gagccgccgc cttttgccgc gcaggttact tcgactttac cgcccggcac
     3481 gatgctcata tggatgacgg cgggggtgtt gtctttggtg ttttggcgtt tgccggccgg
     3541 atcggcgagg acggaagcgc gcagcgtatt gccttcccaa gtgtaggcgc ggcgtacgcc
     3601 ttcgttaacc atctcttcca cgctcatgtc cgcatcccat tggacgttca taccgacttt
     3661 gaggaagacg gttgcgatac ctgtgtcttg gcagatgggg cggttgtttt ccgcacacat
     3721 acggctgttg accaaaatct gcgtcatcgc gtctttggcg gcaggatttt cttccttctg
     3781 ccacgcctta taaagcgcgt cgatgtagtc tttgggatga tagtagctga tgaattggaa
     3841 ggcatcgcaa atgctttgga taaagtcttc ctgtttgatg acggtcatga ttgtgttcct
     3901 tttcaaggtt gtggcggtaa gtgtctgctg ctgttttaaa caaccttttt gaaggctgtt
     3961 ttttatcagg ttgaaatagt gccagcgttt gtacggtaaa gacaatacat aattctaatc
     4021 tcttgattat caatttgttt taataaaata ctgttttact cggattttga tatttgtaat
     4081 ctttgcctgt tgctttgcgg tagggagaaa tcagcaggca gttcattgta ggccgattga
     4141 tgaacaatgc cgtctgaaag ggcttcagac ggcattgtgt tcggacgggc gggcttattt
     4201 tgcggcctgt ccgtcttctt ttgcaaaata tttggcgtaa atcttgtcgt attcgccgct
     4261 ttcgcgtact ttttccaacg catcgttcag cattttgacg gttgcttcgt cgcctttgcg
     4321 taccgcgatg ccgtagtgtt cggtggtgaa gtcgggcagg gtaacgaagt ccatcccttt
     4381 ggccggattg tttttcacat aattggcgat gaccgcgctg tcgctgacca cggaatccaa
     4441 gccgccgttt tccagttctt tgataatcag gggaacgttt tcaaagcgcg cgattttcgg
     4501 attgtcgttg cccaagagtt tggatacgga gaaatcgccc gtgtagccgg ttaccacgcc
     4561 gactttgttc atgtttttca aatcttcgga agaagatact tttttgcctt tcggaacgag
     4621 gacgacttgg gtgatttcaa aatacgggtc gctgaagtcc atagactgtt tgcggtcgtc
     4681 ggtaatggtt acgcccgaca tcacaacgtc cgcatcgccg ttgtttaagg cggggaaaag
     4741 gctgtcccac ggctggtgtt tgaattcgat tttaaaattg cccgccttcg ccatcgcgtt
     4801 catcaaatcc acatcgaaac cttcgacatt gcctttcgag tctaaagatt caaagggggc
     4861 aaactcggcg ttggaagcca cgcggtacac tttgtcgggg ttggcggcag gcgcggcggt
     4921 atctttgccc tgaccgccgc aggcagacag cgcgagcgcg gaacaggcaa gggcggcggc
     4981 aatccatttt ttcatattca tcaaaattcc tttcggtaat caaaaagcct aaaaaatcag
     5041 tttaacataa aattaacgtt ctttttcacg gaaaaacgca taagtaacgt cgtggaacaa
     5101 atccgtatgc gggctgtcct catagctcaa ttctgccaag gcgtcctcaa aagcaagctg
     5161 aatcgactcg accgcgattt cggcggtttc ggcgggaagg gcgcggatta aggctttgaa
     5221 tgcggtggac aggacgtggt tctgcatacg cagcacgtcg ttcgcctctt ccagatattc
     5281 caaacgctgt tcgatgctgc ccatgatgta tcctcgcgaa tctgttaaaa tgcgccatta
     5341 tatcaaaccg cgctttgggc gcgggtttgt ctgcttgcaa agattaagaa ccggaagccg
     5401 aaaatggatg cgaataaaga gccgaattgg gaaaaacgtt tggaaaacct gcgtcagacg
     5461 cgccgtatga aggcagtgcc gacttacgcg ccgctgtccc gcccctctga aaccggaacg
     5521 gacgcgcctg tgccgaaact ggacgatgag agccgagccc gtgtgttgaa ggcgtatctg
     5581 aaaaaatggc aggaggaaca caatgccgcc gttgccgatt gcggcgagga agtcgaaacc
     5641 gacccgatga tcgtccttca ggaaaactgg ctcaacgcgc aggcttccct gcaaatgtcg
     5701 gtttcggaaa aacacatcga atcgcggcgg agaatccggt ttgataccaa aaaagacagt
     5761 gtcgaatttg aaaccggaca gattgaaagc ggggaaaacg ccgacgaggc agaatccggc
     5821 acgaatgagc cggcggtttc ggaaaacggg gaaaacgatg cggaagaaac gttgatgccc
     5881 gtccaaatca atatcctgaa cccgaaagcc gtcaaccggc gcgaagtgtt ctgcctgtcc
     5941 gaacaggagc tgaccgaacg gctgatcaaa cgcctgcgcc cgcatctgac cgataccgtc
     6001 aacggcatca tccgaacggc ggtgcaaaag cagatggcgg ttttcaccta tcagctccag
     6061 cagatgctgc acgagcaggc gggggcggta gtcgaagacg tgttggaaca cgatgtccgc
     6121 aaaatcctca acgacatcaa gtacgagtta aaatacaaac gctgattgcc atgccgtctg
     6181 aaagccgttt cagacggcat ttgcattctc atccgacttg aaagcatcat agaacaggcg
     6241 caaaatatta acaaattaaa caacgggcgc acgctccgtt gccgaacgga caaaaccgag
     6301 gaatcaccga tgagcaaaaa actccatccg caaacgctcg ccatacgcgg cggcaaagaa
     6361 caaaccgaat accgcgaaca caatcaggca ttgtttctga ccagcagctt catgtgggac
     6421 aacgcccaac acgctgccga tttgttttca aaaaaaatca aagggttcac ttatacccgt
     6481 accgccaacc cgaccacagc cgcctttgaa aaacgcatcg ccgctttgga aggtgcggaa
     6541 cgcgcggtcg ccacttcggc gggtatgtct gcgattcagg cggcgttttt cacctttttg
     6601 caggcgggcg accatgtgat ttccagccgc agcctgttcg gcacgaccgt cggctttatc
     6661 aataacatcg ttgccaaatt cggcatcgaa gtgagccacg tgtcgccgac tgatataaac
     6721 gaatggaaag ccgccgtcaa agccaatacc aaactgctgt ttttggaaac gccgtccaac
     6781 cccttaggcg aagtggccga cttggaagcc ttggcggaat tggcgcacgg catcggcgcg
     6841 ctcttggtgg tggacaacag cctgctgtcg cccgtcggct cgcagccttt gaaacacggt
     6901 gcggatattt ccgtttcctc cgcgaccaaa gccatagacg gacacgggcg tgtgatgggc
     6961 ggcgtgttgg cgggttcgga agagttgttg gcgcaggtcg ccatgtattg caactcttgc
     7021 gggctggcga tgtcgccgtt taatgcgtgg cagttgttga gcggcgtgga aaccctgtcg
     7081 ctgcgtatgg aaaaacagtt cgacaacgcc ctgaaaatcg cacaatggct gcaagcgcag
     7141 ccgcaggttc aagccgtgta ctacaccgga ttgtccgacc atcctcaggc ggcgcttatc
     7201 cgcaaacagc aaaacggcgg cggcatcgtc atcggctttg aagttgccga tcaagaagcg
     7261 gcgtggaaag ttgtggacgg cgtggaactc ttttcccgca ccgccaacct cggtgacgtg
     7321 cgctcgacca ttacccaccc gtggacaacc acgcacggca ggatgcagcc cgaagaaaaa
     7381 ctcgccgcca acatccgccc cggattggtg cgcctgtcgg tcggtttgga atatgttggc
     7441 gatttgattg acgatttgaa acaggcactc gcacgctgat tgcgtgtttc agacggcatt
     7501 ggacagcaga tgccgtctga aacccttatc ttatatggca tacacatcaa caataggaaa
     7561 aaaatatgga tgaagcggtt tttgccgatt gggcgttgaa aatctgcctg accggcttga
     7621 ttattttttt gggttttatc gtttggaatt tgggcaagga gtccaaagca ggcaaattcg
     7681 gcatcgccat tttgtttttg gttttgggct tgggcgtgtt cggttttgta ttcaaagaac
     7741 tgctgattaa gtttttggta ttgccgaaat aggctgccgc ctgtcaaaat gctgtctgaa
     7801 atcataaaaa gtaatctgca ccctaaaagt ttgatttaac cgccaactga ttaaggtgcg
     7861 gatttttatg acaa
//
LOCUS       DAWGRZ010000092         7848 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000092 DAWGRZ010000000
VERSION     DAWGRZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7848
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.092"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            91..1320
                     /gene="sstT"
                     /locus_tag="WHT44_001349"
     CDS             91..1320
                     /gene="sstT"
                     /locus_tag="WHT44_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ2533528.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPFPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATINMAGAAITITVLAMAAAHTQGITVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGIDNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            1575..3020
                     /locus_tag="WHT44_001350"
     CDS             1575..3020
                     /locus_tag="WHT44_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ2533529.1"
                     /translation="MFKRSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPVVSE
                     KETEAKEDAPQAGSQGQGAPSAQGGQDMAAVSEENTGNGGAATADNPKNEDEAQNDMP
                     QNTAGTDSLTPNHTPASNMPAGNMENQAPDAGESAQPENKPDMANAADGIQGDDPSAD
                     GENAGNTAAQGTNQAENNQTAGSQNPASSTNPNATNGGGDFGRTNVGNSVVIDGPSQN
                     ITLTHCKGDPCNGDNLLDEEAPSKSEFEKLNESERIEKYKKDGKDKFVGLVATTVKME
                     GINKYIIFYTDKPPTRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNIFAP
                     EGNYRYLTYGAEKLSGGSYALSVQGEPAKGEMLAGTAVYNGEVLHFHTENGRPYPTRG
                     RFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGRFYGP
                     VGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(3096..3311)
                     /locus_tag="WHT44_001351"
     CDS             complement(3096..3311)
                     /locus_tag="WHT44_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533530.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(3304..3519)
                     /locus_tag="WHT44_001352"
     CDS             complement(3304..3519)
                     /locus_tag="WHT44_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ2533531.1"
                     /translation="MKEYKVVIYQESQLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(3594..4937)
                     /gene="argG"
                     /locus_tag="WHT44_001353"
     CDS             complement(3594..4937)
                     /gene="argG"
                     /locus_tag="WHT44_001353"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ2533532.1"
                     /translation="MSQNHTILQSLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEVSPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKK"
     gene            5250..6050
                     /locus_tag="WHT44_001354"
     CDS             5250..6050
                     /locus_tag="WHT44_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ2533533.1"
                     /translation="MNAFNLIIIGDEILHGSSQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGMPTFVTGGIGATPDDHTRQAAAAALDLPVVRHSEAAKFIEG
                     ITQKRGEPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYADRFNQTERGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            6090..7076
                     /locus_tag="WHT44_001355"
     CDS             6090..7076
                     /locus_tag="WHT44_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ2533534.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            7733..7848
                     /locus_tag="WHT44_001356"
                     /pseudo
     CDS             7733..7848
                     /locus_tag="WHT44_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         1905 a         1982 c         2184 g         1777 t
ORIGIN      
        1 aatacagccg gaaaataaaa aaaaacacag agaatgagtt ttcttttaaa atacaacatt
       61 ttttaacata tcacaacata aggaagaatt atggcgttcg gcaaatcctt atttcatgcg
      121 atcggccgcg tcagcctggt caggcagatt gccgccggtt tggcgttggg catcgtaatc
      181 ggttcggttt ccccgcaact gggcttggcg gcaggcttgt tcggcagcct gtttgtcggt
      241 gcgctcaaag cggtcgcgcc ggttttggta tttattttgg tggcggccac aatcgcgcag
      301 caccaaaaag gcaacaaggc gcatatcagg ccgattatcg tcctttacct catcggtacg
      361 ttttccgcag ccctgaccgc cgtcatcgcc ggtatggttt tcccgacgca cattgttttg
      421 gcgggcgcgg gcgatgtgtc cgccgcgccg ccttccggca ttgtggaagt attgaaatcg
      481 ctgctgatga acctggtcgc caacccgatt aacgcgattg ccaatgccaa ctatatcggc
      541 attttggctt gggctttggt tttgggcgcg gcgttgcgga atcacggttc ggacgttacg
      601 cggcaggtcg ttgccgattt ggcggaagcg gtttccaccg tcgtgaaatg gattatccgt
      661 tttgcccctt tgggcatttt cgggctggtg tcgtccacaa tcgcggagac gggtttcggc
      721 gcgctggcgg ggtacgcgaa gctgcttgca gtgctgttgg gctgtatggc gtttatcgcg
      781 ctggcggtca atcccgccat cgtgtggtgg aaaatccgcc gcaacccttt cccgctggtg
      841 tttacctgcc tgcgtgaaag tggcgtttat gccttcttta cccgttcttc cgccgccaat
      901 atccctgtga acatggcatt ggcgaaaaag ctgggtttgc acgaagacac ttactccatc
      961 tccattccat tgggtgcaac catcaatatg gccggtgcgg caatcaccat taccgttttg
     1021 gcgatggctg cggcgcacac gcaaggcatt acggttgact tcgccactgc cctgctgctg
     1081 agcctggtgg ctaccgttag cgcgtgcggt gcatccggtg tggccggtgg ctcgttgctg
     1141 ctgattcctt tggcgtgcag cctgttcggt atcgataacg atgtggccat gcaggtggtt
     1201 gccgtcggct tcattatcgg cgtgattcag gattcggcgg aaactgcgct gaactcttcg
     1261 accgacgttt tgtttaccgc tgccgccgat ttgggccgac agaggaaccg ggcggaataa
     1321 accaagctat gccgtctgaa gcccgtttgg cgttcagacg gcatattttt acaattttat
     1381 aggcatacgg cggaaacaat atttattgaa tatgtttcaa cacacagggc ggcacataaa
     1441 gcacagccct atgtgtcgcc ctgatttgga aaggttgcgc tcttcccaaa taaagctgat
     1501 tccaccgccc cgaaggacgg atgtccaagt ggcggggttt aaaccaaaaa ggaaatacga
     1561 tgaaggagat gatgatgttt aaacgcagcg taatcgcaat ggcttgtatt tttgcccttt
     1621 cagcctgcgg gggcggcggt ggcggatcgc ccgatgtcaa gtcggcggac acgctgtcaa
     1681 aacctgccgc ccctgttgtt tctgaaaaag agacagaggc aaaggaagat gcgccacagg
     1741 caggttctca aggacagggc gcgccatccg cacaaggcgg tcaagatatg gcggcggttt
     1801 cggaagaaaa tacaggcaat ggcggtgcgg caacagcgga taatcccaaa aatgaagacg
     1861 aggcgcaaaa tgatatgccg caaaataccg ccggtacaga tagtttgaca ccgaatcaca
     1921 ccccggcttc gaatatgccg gccggaaata tggaaaacca agcaccggat gccggggaat
     1981 cggcacaacc ggaaaacaaa ccggatatgg caaatgcggc ggacggaata cagggggacg
     2041 atccgtcggc agacggggaa aatgccggca atacggctgc ccaaggtaca aatcaagccg
     2101 aaaacaatca aaccgccggt tctcaaaatc ctgcctcttc aaccaatcct aacgccacga
     2161 atggcggcgg cgattttgga aggacgaacg tgggcaattc tgttgtgatt gacggaccgt
     2221 cgcaaaatat aacgttgacc cactgtaaag gcgatccttg taatggtgat aatttattgg
     2281 atgaagaagc accgtcaaaa tcagaatttg aaaaattaaa tgagtctgaa cgaattgaga
     2341 aatataagaa agatggaaaa gataaatttg tcggtttggt tgctacaaca gtcaaaatgg
     2401 aaggaattaa caaatatatc atcttttata cggacaaacc ccctactcgt tctgcacggt
     2461 cgaggaggtc gcttccggcc gagatgccgc tgattcccgt caatcaggcg gatacgctga
     2521 ttgtcgatgg ggaagcggtc agcctgacgg ggcattccgg caatatcttc gcgcccgaag
     2581 ggaattaccg gtatctgact tacggggcag aaaaattgtc cggcggatcg tatgccctca
     2641 gtgtgcaagg cgaaccggca aaaggcgaaa tgcttgctgg cacggccgtg tacaacggcg
     2701 aagtgctgca ttttcatacg gaaaacggcc gtccgtaccc gactagaggc aggtttgccg
     2761 caaaagtcga tttcggcagc aaatctgtgg acggcattat cgacagcggc gatgatttgc
     2821 atatgggtac gcaaaaattc aaagccgcca tcgatggaaa cggctttaag gggacttgga
     2881 cggaaaatgg cggcggggat gtttccggaa ggttttacgg cccggtcggc gaggaagtgg
     2941 cgggaaaata cagctatcgc ccgacagatg cggaaaaggg cggattcggc gtgtttgccg
     3001 gcaaaaaaga gcaggattga tgctgatgct gtctgaagcg ggggcgttca gacggcattg
     3061 tgatgtttgg gttgcgggtc cggttcaagt ccgcatcagt tgataaaccc gtcgtctttc
     3121 attttctttt cggaaaaata ctggccgaat accagcaagg cggcgaggat gatgacaaac
     3181 agccccgcca tagtcagtgt gattttgaag aacaattccg cttcgagaaa agaaaaccaa
     3241 agttgcacaa tcagcagcag cacccagccg gcaaacagtc cgagacaggc ataaacgcca
     3301 agcttaaaca cggtcccgct ccaaaatgac gacgtaggcc tcgcgtttga aaaacaacag
     3361 catacggcgc aaatcttttt ccatcgttac aacccgccag ccttcggggg tttgtttgtt
     3421 gaggaacgcg ctgaaattga cggggttgac ctttgccgcg ccgaaaaaca ggctggacaa
     3481 ctggctttcc tgataaatga cgactttgta ttctttcata ggattccttt taacataagg
     3541 gatcatcttt tcagacgacc ccttaagttg ctaatgatgc aaaacagcaa accttatttc
     3601 ttattgccca actgcggcaa taccgagcct tcgcccagcg agagcaaacc gctttgcgag
     3661 tagataccca gtttggcgcg ggtgtcggtg atgtcaaggt tgcgcatcgt gagctgtccg
     3721 atgcggtcta gcggggtaaa cgccgcacct tcgacttttt ccatactcag gcgttcgggt
     3781 tggtaggtca gattgggcga ttcggtgttc agaatcgagt agtcgttgcc gcgccgcagt
     3841 tcgagggtaa cttcgccggt aacggcttta gcgacccagc gttgggcggt ttcgcgcaac
     3901 atcagggctt ggctgtcgaa ccagcggcct tggtagagca aacgtccgag gcgcaggccg
     3961 ttgatgcggt attgttcgat ggtgtcttcg ttgtggatgc cggtgaccaa gcgttcgtag
     4021 gcgatgtgga acaacgccat tcccggggct tcgtagatgc cgcgcgattt ggcttcgatg
     4081 atgcggtttt cgatttggtc gctcataccc agaccgtggc ggccgccgat gcggttggct
     4141 tcgaggaaga gttcgacggg atcggcgtat tctttgccgt ttagtgcaac cggcacgcct
     4201 tcttcaaagc gtacgctgac ttcttccggg ctgacttcga cgttttcgtc ccaaaaggca
     4261 acgcccataa tgggtttgac gattttgatg cccgagttca aaaattccaa gtctttggct
     4321 tcgtgggtgg cacccaacat attggaatcg gtggagtagg ctttttccac cgacattttg
     4381 tagttgaagc cgttggcaat cagaaattcg ctcatttcgt gacggccgcc gagttcgtcg
     4441 ataaattgct gatcgagcca gggtttgtag attttcagcg cgggattggt gagcaaaccg
     4501 tagcggtaga aacgctcgat gtcgttgcct ttgtaggtgc tgccgtcgcc ccaaatattc
     4561 acatcgtctt ctttcattgc ggaaacaagc atagtgccgg ttacggcgcg gcccagaggc
     4621 gtggtgttga aataggcgat gccgccggtg gaaacgtgaa acgcgccgca ttggatggcg
     4681 gcgatgcctt cgtgtgccaa ctgcgcgcgg cagtcgatta agcgggcgtt ttccgcaccg
     4741 tattccatcg cttttttggg aatggcgttg tagtcgtctt cgtcgggctg gccgaggttg
     4801 gcagtgtagg cataaggcag cgcgcctttg agtttcatcc acaacagcgc ggcagaggta
     4861 tcaagaccgc cggagaaggc gatgccgact ttctgaccga cggggaggga ttgcagaatg
     4921 gtatggtttt ggctcataag ggtatccttc ggacttgaaa aaaggagttc attatcttat
     4981 aaatataacc cattgtcagc cgttaaggct gtctgaaaag aagctttgcc cgtttcgctg
     5041 aaaatctttt cagacggtct ttacgccatc ctcaacctga ctccctcccc tgcgggggag
     5101 ggttggggag agggtacaga cagcaggcct taagcaaacg ctgcctgaaa aacgcaagct
     5161 cgaccctctc cctaaccctc tcccacggga gagggaacaa atcacagcac catcgacatt
     5221 ttcagacggc atcggtaaag aaaccatcta tgaacgcgtt caacctcatc atcatcggcg
     5281 acgaaatctt acacggcagc agccaagaca agcatttcgc ctttttcaaa tccctgctcg
     5341 aatccaaagg gctgaagctc aatcaggtgc aatacctgcc cgacgaaccc gatttgctgg
     5401 tcaggcaact gcgccgcagc ttttcagacg gcatgccaac tttcgttacc ggcggtatcg
     5461 gcgccacgcc cgacgaccac acccgccaag ccgcagccgc tgctttggat ttgcccgtcg
     5521 tccgccattc tgaagccgcc aagtttatcg aaggcataac ccagaaacgc ggcgagccgc
     5581 ttgattcgcc ggaacacgcc caacgcctga aaatggcaga ttttcccgaa ggcgcggaat
     5641 tggtgcccaa tccgtttaac aacatcgccg gattttctat ccgcgagcat tatttcttcc
     5701 ccggctttcc tgtgatggcg cacccgatgg cggaatgggt attggaaact tattacgccg
     5761 accgcttcaa ccaaaccgaa cgcggcagcc gcagcgtgta tgtgtttgag cagcccgaat
     5821 cgcgcattac gccgcttatg gagcatatcg agcaaactta tcccggcgtg cgttcgtaca
     5881 gcctgcccag cgtcggttgg acgcattcag acggcacgca ggtcaaaccg cacattgagt
     5941 tcggcatcaa agccgaagga gaagccgtca accttttgga cgcggcgtgg gcggaagtgt
     6001 tgcacagctt ggacggattg ggagcagagc tgaaaaatcg ggtaaactga aaaccgtttg
     6061 cccaaatcgg acaatattta aggaaaacca tgcaataccg aatcagaaga gaaaacgaag
     6121 cacccgaagc aaaaaatgcg ggggaaacct tatgggagcg cgacatcatg cgcgaagtgc
     6181 tgttgtccgc ctatcaggac aggcgcaggg agcggatgtg gaaaaacatc tggcgcgccg
     6241 tcagcaccct gattctggtt gccctgattg caggcatttt ccgaaaagac gaagccgcat
     6301 tgcagttggc gggcaacacg ccgcataccg cagtcgtcaa tctgtacggc gaaatcggca
     6361 acggtgtaga agaccaggtc aaaaaactca aagacggtat ggaggccgcc tacaaaaacc
     6421 cgcaggcaaa agccatcgtc atccgcgcca acagccccgg cggttcgccc gtcgtgtcca
     6481 acaccgcttt tgaagaaata cgccgtctga aggcgcagca ccccggcatt cccgtttatc
     6541 tcgtggcgga agatatgtgc gcgtccggct gctactacat cgcggcggcg gcggacaaaa
     6601 tctatgccga cccgtccagc atcgtcggca gcatcggcgt tatcggcagc agtttcgacg
     6661 cgaccggcct gatggaaaaa atcggcgtga aacgcagggt taaaattgca ggcagcaaca
     6721 aaggcatggg cgatccgttt tcgcccgaaa cgcccgaaca gtcaaaaatc tgggaagaaa
     6781 tgctgaccgg catacacggc gagttcatca aagccgtcaa aaccggacgc ggcggccgcc
     6841 tcaaattccg acaatatccc gacgtgttca gcggtcgcgt gtacacaggc gcggatgcgc
     6901 tgaaagtcgg gctggtggac ggactcggca atatttacag cgtggcgcga gacgtggtca
     6961 aagcccccga tgtggtggat tacactccga aggacgattt cggcagaatc ttgggcagac
     7021 gcttcggcgc agaactcaaa gcaagcgtgc gcgaagccct gcaggccgtc cggtagcgtt
     7081 gtttacagta tttctaaggg aataccaaaa taaaaatata cacaaaaaca ctcacctccc
     7141 cagtatgctg ccactaagtc agccgcgtca ctatttaccg tgcacttaaa gccgtaaaag
     7201 gcaggttgtc cccgggaggt tttgtgcatc ctgataccga tcggttgttg ttgctcaaag
     7261 gacagaaggc cgctgataaa cgagattacc tgtttgtcgc tattgacgat ttttatactc
     7321 tgccattttg ccggacaaaa ccgcagacag tgctgccaag tttctgaccg aacatctgtc
     7381 cgacccctgc ttgtacctga ttgagtacgt ttactctgac aatgataggt aatataaaga
     7441 gccgtccaac atgctttcgg tgcagcttgt tatgataatg ggattggttg gaggcttgcc
     7501 cgatttgctt gtccgcagac caacggtaag gcggagcggg ttatccgtac cttgatggag
     7561 atgtggcatg aggaacagtc gtttgacagt ccggaacatc ggcaaaagga gttgtgctgc
     7621 tttattaatt taggggctgc cctagatcac tagggaaatt caaattaagt tagaattatc
     7681 cctatgagag aaagtcgtct aagacggtat aaacaaaata aactcatttt gttatcgtgg
     7741 ctatgatgta ttggatgcgg gcgaatttag ccatttccgt atcaatcaca gcacacattt
     7801 tgccgaacga caaaaccata ttaatggaac tgagaacttt tggaatca
//
LOCUS       DAWGRZ010000093         7799 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000093 DAWGRZ010000000
VERSION     DAWGRZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.093"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(453..2489)
                     /locus_tag="WHT44_001357"
     CDS             complement(453..2489)
                     /locus_tag="WHT44_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ2533535.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAV"
     gene            complement(2577..3449)
                     /locus_tag="WHT44_001358"
     CDS             complement(2577..3449)
                     /locus_tag="WHT44_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ2533536.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNHPAGSETL"
     gene            3719..6109
                     /gene="gyrB"
                     /locus_tag="WHT44_001359"
     CDS             3719..6109
                     /gene="gyrB"
                     /locus_tag="WHT44_001359"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ2533537.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKI
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINNYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPNEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVATLITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIISDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            6206..7591
                     /locus_tag="WHT44_001360"
     CDS             6206..7591
                     /locus_tag="WHT44_001360"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEZ2533538.1"
                     /translation="MISIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNSLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPSGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(7662..7760)
                     /locus_tag="WHT44_001361"
     CDS             complement(7662..7760)
                     /locus_tag="WHT44_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ2533539.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
BASE COUNT         1782 a         2162 c         2194 g         1661 t
ORIGIN      
        1 ccgtctgaag cgttgtttga ccgtttcaga cggcattttt atcaaattca aaaatattcc
       61 gtcattcccg cgaaagcggg aatctagaac gtaaaatcta aagaaaccgt tttatccgat
      121 aagtttccgc accgacagac ctggattccc gcctgcgcgg gaatgatggg atttctgttt
      181 ttgatttttt tgtttttggg gaatgacggg atttgagatt gcgggcattt atcgggtaaa
      241 acggaaatta aacgttgcga aaatttatcc ggaatcacag caacttttcc gcgtcattcc
      301 cacgaaagtg ggaatctaga acgtaaaatc taaagaaacc gtttttcccg ataagtttct
      361 gcaccgaccg gtctggattc ccgcctgcgc gggaatgacg gtgcggatgt ttttctatcg
      421 aatccgccat attttttact tcaaccctgc cgtcagaccg cgttgtcgaa accgctgtgg
      481 cgcaagagtg cgtctatgct cggttcgcgg ccgcggaagg ctttgaagga ttctgccgcg
      541 ctgcgcgatc cgccgacggc gaggatttcc tgccaaaagc gtttgcctgt ggcggcgaca
      601 tcgtcgcttt cttcaaaggc ggcgtatgcg tccgcgctca atacttccgc ccacgcgtag
      661 ctgtaatagc ctgcggaata gccgcctgcg aagatgtggc cgaagctcaa ggcgaagcgg
      721 ttgtattcgg gcggctggat gacggcgact tttttgcgca cgctgtctaa aacctgttgc
      781 cagtttttca gacggccttc gtcgtcttcg ctgtaaatca tcatatcaaa gagggcgaac
      841 tccatttgcc ggacgaggaa catgccgcgt tggaagtttt tggcggcgag cattttgtcg
      901 aagagttctt tcggcagggg aacgccggtt tcttcgtggg ctgacatttg tgccaagaca
      961 ttgtattccc aaacgaaatt ttccataaac tggctgggca gttcgaccgc gtcccattct
     1021 acgccgttga tgccggatac gcccagttcg tccacttggg taagcaggtg gtgcagcccg
     1081 tgtccggttt cgtggaagag gatgaggatt tcgtcgtggc tcaggcgggc ttccctgccg
     1141 ccgacgggtg gggcgaagtt gcagacgagg taggcggtgg gcagttgcag cgtgccgtct
     1201 gaaaaacggc ggcggccttt gtagtcgttc atccacgcgc cgccgcgttt gccttcgcgt
     1261 gcgtacaaat ccatataaac gccgcctatg gtttcgccgt tttgttgcaa ttcaaaatag
     1321 cgcacgtctt tgtgccagac ggggacggtt ttttcggtaa atccgatgcc gtagagtttt
     1381 ttgatttggg cgaacagtcc gtttaatact ttgccgacgg ggaagtattt tttgacttcg
     1441 gtttcgctga acgcgtattt ggcttcgcgc agtttttcgc tggcgtagcc caagtcccac
     1501 ggttgcaaat cggcgaggtt caggctttcg cgggcgaagg ctttgacttc ggcgaggtct
     1561 ttttcggcgt agggtttggc gcggcgggcg aggtcgtgca ggaagtttaa aacttgttcg
     1621 ggcgtgtccg ccattttggt tgccagcgac aattcggcgt agtttttgaa gccgagcagt
     1681 ttggcggttt gcagggcgtt tgcgagcgtg cggtcgatgt tggcggtgtt gtcgaatttg
     1741 ccgtcgtctg aaagttcgct ggcgcgggta acgtaggcgc ggtagatttg ttcgcgcagt
     1801 tcgcggttgt cggcgtattg gatgacggcg aggtagtgtg gaatctgcaa gccgattttg
     1861 tagcctgttt tgctttcgct ttgcgcggcg gcggcaaaca tggcgagcgc gtcttcggga
     1921 atgccggcaa gcggtgcggc atcgtcaaag taaatgccga acgcgtcggt cgcgtctagg
     1981 acgttttggg agaatttggc ggaaagttgc gcgccttcgg tttgcagttt tgccagttct
     2041 gcctgctgtt cgggcggcag ttccgcgccg ctgaggacga aatcgcgcag atcgtggttg
     2101 agtttggttt tttgtgcggg ggagagggtg tcgaattcgg gggaattttt gatggttttg
     2161 aagcggttgt acagctcgat gtcttgtccg atttcggtga agaagacggt gatttcgggc
     2221 atcagttcgt tatagacggc gcgcagttcg ggcgtgtcgg cgacggagtt gaggtgcgac
     2281 accacgcccc aaatcctgcc gacgcgttcg gtgatgccgg tcaggggttc gacagtgttt
     2341 gcccagccgg tgtgcgtttg ggctttgatg gcggcgattt gttcgcgcgc ttcggcgatg
     2401 gcggtttgca gggcgggttt gatgtcttcg gttttgattt gatcaaaacg gggttcttcg
     2461 cccaaatgga gcagtgcgtt gtcagtcata agatgggttt cctttgcgtg ttgtgtttca
     2521 gacagcatat cggcaatatg ccgtctgaag ccggaaatgg ggtcggtcgg ggaaagttaa
     2581 agtgtttcgg agccggcggg gtgatttaac aggcggtatt gtacgcgcaa aaattcgtcc
     2641 ataaccgcct gttggatttc cagccgcttt gaaacggggg aagcgaagcg gacgatgatg
     2701 cggtatgcct tgtcatcgta cggcacgcgg gtaacgcgcg gtctggcggc gggcgtgata
     2761 aacagttttt ccgcctgcac gttttccaaa tgccgttgga tggcggggat gtagggcgcg
     2821 cacaagggct cgagtacggc tttcagacgg catacggctt catccgaatc caaatggatg
     2881 ggaacgggga tttcgaccgt atggatgaca tagtcgccca aaatattgtc gcggcgcacg
     2941 gggtggctca acaacaggct gttggggaaa gaaacggtgg ttcccgcaag ctgtccgacc
     3001 aaggggttcg gaccgacctg catcatcagc gtgttcaaca ggttgatgtc gaccacgcgc
     3061 ccgcgcaggc cgttgatttc gatatagtcg ccgaccgagt attgctgggt ggcagacctt
     3121 aaaatactgc ccgacagaca cataatcagt tccttcgtcg ccacgacgac cgccgccgcc
     3181 accgcaaaca tcgacaaagc cagcgtttgg atttgcgccg accagataaa tgccagcgaa
     3241 aacagcacca aaagcagcgt tatattgcgg ctggcaacca aaaaccgccg cttgctttcg
     3301 atgccgaaat ccggatgccg tttgaagtgg atattcaaca gaagggcgcg cgccagcagc
     3361 aaagccgcaa ccgccgccac ggattcgacc gcctccgcac gtatcgggac ggcaccgagc
     3421 caagtgtcca acatattcca tatttccatt tccgcgcccc tgtccgaaaa gtatagggtg
     3481 tagattttag tggcaaaaaa acgcttttgc cactttacgg gacaatcccc gacctgatgc
     3541 cggaggcaaa cgtaccgttt catcttttcc cggcggcaca ctgatgcgga taacgctgaa
     3601 ttaggcggca aaatcggcta taatacacaa ttatatatgt cgtcccgccc gctttttcta
     3661 caaggtgcgg acttgttttg gcggcatcgc aaaatctttt caaaatccgg caaaaaatat
     3721 gactgaacaa aaacacgaag aatacggcgc cgacagcatt caggtgctcg aaggcttgga
     3781 agcggtacgc aaacgccccg gcatgtacat cggcgacacg caggacggca gcggtctgca
     3841 ccacatggtg ttcgaagtat tggacaacgc cattgacgaa gcactcgccg gacattgcga
     3901 caaaatcacg gtaacgatac acgccgacca ttccgtcagc gtcgccgaca acgggcgcgg
     3961 tatgcccacc ggcatccacc cgaaagaagg acgctccgcc gccgaagtca tcatgaccgt
     4021 attgcacgcg ggcggtaaat tcgacaacaa cagctacaaa atctccggcg gcctgcacgg
     4081 cgtgggcgtg tccgtcgtca acgcgctgtc cgactgggta acgctgacca tctaccgcga
     4141 cggcaaagaa cacttcgtcc gcttcgtgcg cggcgaaacc gaagagccgc tgaaaattgt
     4201 cggcgattcc gataaaaaag gcacgaccgt gcgcttcctc gccagtacgg aaaccttcgg
     4261 caacgtcgaa tacagcttcg acatccttgc caaacgcatc cgcgaacttt ccttcctgaa
     4321 caacggcgtg gacatcgaat tgaccgacga gcgcgacggc aaacacgaaa gcttcgccct
     4381 ttccggcggc gtggcgggtt tcgtgcaata catgaaccgc aaaaaaacgc cgttgcacga
     4441 aaaaatcttc tacgcgttcg gcgaaaaaga cggcatgagc gtcgaatgcg cgatgcaatg
     4501 gaatgacagc tatcaagaaa gcgtgcagtg tttcaccaac aacatcccgc aacgtgatgg
     4561 cggtactcac ctgaccgcac tgcgccaagt gatgacccgc accatcaaca actatatcga
     4621 agccaacgaa gtcgccaaaa aagccaaagt ggaaaccgca ggcgacgata tgcgcgaggg
     4681 tttgacctgc gtgttgtccg tcaaactgcc cgaccccaaa ttctcgtccc aaaccaaaga
     4741 caaactggtt tccggcgaaa tcggccccgt tgtcaacgaa gtcatcagcc aagccctgac
     4801 cgacttcctc gaagaaaatc cgaacgaagc caaaatcatc accggcaaaa tcgtcgatgc
     4861 cgcccgcgcg cgcgaagccg cccgcaaagc acgcgaaatc acccgccgca aaggcgtgat
     4921 ggacggcttg ggactgcccg gcaaactcgc cgactgccaa gaaaaagacc ctgccctgtc
     4981 cgaactctac ctcgtcgagg gcgactccgc aggcggttcc gccatgcaag gccgcgaccg
     5041 caaattccaa gcgattttgc cgctcaaagg taaaattttg aacgtcgaaa aagcacgttt
     5101 tgaaaaaatg ctggccagcc aagaagtcgc cacgctgatt accgctttgg gcgcgggcat
     5161 cggcaaagaa gaattcaatg ccgaaaaact gcgttaccac cgcatcatca tcatgaccga
     5221 tgccgacgtg gacggcgcgc acatccgcac cctgctcctg accttcttct accgccaaat
     5281 gcccgagctg gtcgagcgcg gctacatcta tatcgcccag ccgcctttgt ataaagcgaa
     5341 atacggcaaa caggaacgtt acctcaagga cgagttggaa aaagaccaat ggctgctcgg
     5401 tcttgccttg gaaaaagcca aaatcatttc agacggccgc accatcgaag gcgcagaact
     5461 tgccgacacc gccaaacaat tcctgttggc aaaaaccgtc atcgaacagg aaagccgctt
     5521 cgtagacgaa ctcgtcctgc gcgccatgct acacgcgtcg cccattgatt tgacgtcgtc
     5581 tgaaaacgcc gataaagccg ttgccgaact ttccggtctg cttgacgaaa aagaagtcgc
     5641 cctcgaacgc atcgaaggtc atgaaggaca ccggttcatc aaaatcacgc gcaagctgca
     5701 cggcaacgtc atggtcagct acatcgaacc caagttcctc aacagcaaag cctaccaaac
     5761 cctcacccaa accgccgccg cgctcaaagg catggtcggc gagggtgcca agctttacaa
     5821 aggcgaaaac gggtacgacg cggacagctt tgaaaccgct ttggacatct tgatgagcgt
     5881 tgcccaaaaa ggtatgtcca tccaacgata caaaggcttg ggcgagatga accccgagca
     5941 gctgtgggaa accacgatgg atcccgccgt gcgccgcctg ttgaaagtgc gcatcgaaga
     6001 tgccattgcc gccgacgaag tgttcgttac gctgatgggc gacgaggtcg agccgcgccg
     6061 tgcctttatc gaaaacaacg cgctgattgc ccaaaatatc gacgcataag tgccgtttta
     6121 aaaaaggaga cgggcatcgt gccgcgtctc ctttttggtt ggtcaaacgg aacctgtgcc
     6181 gtctgaaaaa ccgtcggagc aaaatatgat cagcattttc gatattttca aaatcggtat
     6241 cgggccttcc agttcgcata cggtcggccc gatgaaggca gccgccgcct ttgcggcagg
     6301 tttggatgca caggctgttc gcatcgtcat cgacatttac ggctcgctcg cactgaccgg
     6361 atacggacac ggtacatttg acgcgctgat gctcggtttg gaaggcagcc tgccgcacga
     6421 catcccgctt gccggcattc ccgaacgcct cgaacgcatc cgcacgcagc acatcctccg
     6481 gctcaacggg caagaaatcc gcttcatccc cgaccgcgac ctgaacatac tcggcaatca
     6541 agtgctgccc aaacacccca acagcctgcg ttttaccgcc tatgcttcag acggcacggt
     6601 attgaatgaa caggtttatt attcggtcgg cggcggcttt gtcgttaccg aagaagattt
     6661 tgaccggcag gcggaaacgg aaaaagccgt tccctatccc tataccagtt gcgccgaact
     6721 gcttgcccga tgccgtctga accggctcga catctccgaa gtcgtgttgg caaacgaagc
     6781 cgcgcttgcc ggatgcggcg aagccgaaat ccgccgccgc gccgctgccg ttgccgaggt
     6841 tatggaaggc tgcatcaaac gcggcttggg tgcggacggc gaactgccca gcggattgaa
     6901 cgtccgccgc cgcgccccgc agcttgccgc caagctcaaa gtcctgcgcg aaaccgaaat
     6961 cgtcaacacc cagctctggc cgatggtgta cgccatggcg gtcaacgaag aaaacgccgc
     7021 cggcggacgc gtcgttaccg caccgaccaa cggcgcggca ggcatcattc ccgccgtatt
     7081 gcactatttc cgcaagttca atccgcacgc cacacaggaa cgcgtcgaaa acttcctgct
     7141 caccgcaggc gcaatcggca tcctctacaa gaccaacgcc tccatttccg gtgcggatgt
     7201 cggctgtcag ggcgaagtcg gcgtagcgtg ttcgatggcg gcgggcgcat acgccgaagt
     7261 catcggcggc acgcccaaac aagtggaaaa cgccgccgaa atggcgatgg aacaccattt
     7321 ggggctgact tgcgaccccg tcggcggact ggtgcaaatc ccctgcatcg agcgcaacgg
     7381 catcgccgcc gaaaaagccc tcaaactcgg cacgctcgcg cttttggaag acggcacgga
     7441 caaaaaagtc tcgctcgacg aagtcatccg aaccatgctg caaaccggcc gggatatgaa
     7501 ggcgacctac aaagaaacct cgcttgccgg actcgccgcc acgctccgga aaaaagccgt
     7561 ccccgtatcc gtgcgcgtgg tcgagtgctg aggatggaca gaaacgaaaa tgccgtctga
     7621 aaacagtttt ttagacggca tttcatttat ttcttgcggc tttaccgttt cagtgccagc
     7681 gtcaacacgc ccgccgtaac cagccccaag cctatccatt cctgcgtgtt cgggcgttcg
     7741 tctaagaaaa ccaccgccat caggaatgca aaccggcgtt gtttgcagca tccggcaag
//
LOCUS       DAWGRZ010000094         7676 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000094 DAWGRZ010000000
VERSION     DAWGRZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7676
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.094"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(9..425)
                     /gene="dksA"
                     /locus_tag="WHT44_001362"
     CDS             complement(9..425)
                     /gene="dksA"
                     /locus_tag="WHT44_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ2533540.1"
                     /translation="MAKLTEQDILNWSGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(572..1363)
                     /locus_tag="WHT44_001363"
     CDS             complement(572..1363)
                     /locus_tag="WHT44_001363"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ2533541.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGYRIYIANRGAEKRERLEKEL
                     GVETSATLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVGTLSRYLGG
                     TRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLDDEEKMHGI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1412..1819)
                     /locus_tag="WHT44_001364"
     CDS             complement(1412..1819)
                     /locus_tag="WHT44_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ2533542.1"
                     /translation="MEIRAIKYTAMAALLAFTVAGCRLAGWYECSSLTGWCKPRKPAA
                     IDFWDIGGESPPSLGDYEIPLSDGNRSVRANEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQGGFDCLEKQGLRRNGLSERVRW"
     gene            complement(1819..2505)
                     /locus_tag="WHT44_001365"
     CDS             complement(1819..2505)
                     /locus_tag="WHT44_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ2533543.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQAGREPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAVKHGVAPEEAVALAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2625..3668
                     /locus_tag="WHT44_001366"
     CDS             2625..3668
                     /locus_tag="WHT44_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ2533544.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNIGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3831..5057
                     /locus_tag="WHT44_001367"
     CDS             3831..5057
                     /locus_tag="WHT44_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ2533545.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5343..7493
                     /gene="yccS"
                     /locus_tag="WHT44_001368"
     CDS             5343..7493
                     /gene="yccS"
                     /locus_tag="WHT44_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ2533546.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIITTVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAILLFQIVLPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDSPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETSSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1889 a         2567 c         1667 g         1553 t
ORIGIN      
        1 tgccgccatc agtcggcaaa ctgttttttc atcctctctc ggcgttcttg ggactcaaca
       61 gataaagtgg ctgtcgggcg tgccagcagc cgcttcaaac cgataggctc tcccgtatcg
      121 gcacagaatc cataatcccc ttcatcaata ttgcggatgg tcgcctgtat tttactgaga
      181 agttttcgtt cccgatcgcg ggtacggagt tccaatgcgt actcttcttc ctgtgtggca
      241 cggtcggcag gatcgggggc tgattcgtgt tcttggagat gccctgtcgt agcggaagca
      301 ttttcgatga gttcgtcttg catttttacc agcaattcgc ggaaaaaagc caaatggtcg
      361 tcattcatat aatcgtcttc cggcccgctc caattcaaaa tatcttgttc tgtcagcttt
      421 gccataatgt atatcctctc ggttcaccgc aaaaaacaaa gcaccacaaa gtggctttat
      481 tcctttgatt tttataaggc ttattgccta aggcgttttt gaaaacgcgc atcataacac
      541 gttttggatt ggttttttta ttttctttac attattgata ttgccgttcc atttcctgcg
      601 aacggcgcac acaggcacaa acgccctcgc ttatggcttc ggcgacacga tgccgcctga
      661 aagcttccac ggcttcgtgg gttgtcccgc cttttgacgt tacatttttt tgaagcttct
      721 cgaaatcttc acccgtctgc tcggcaaggg caaccgctcc tttaaacgtt gccagactga
      781 gcgcgcgtgc ttctgccata tcaaaccctt gtcggatggc ggcattttgc aatgcgtcca
      841 gcagataaaa cacataagcc ggtccgctgc cgctgatgcc ggtaatgccg tgcatttttt
      901 cctcatcatc caaccaaaca gtcaaaccga ctgatttcat gattcgatcg gcaatcctgc
      961 ggtctgtttc cgatacttcc gcttcggcat acataccaga tacgcccagc ccgatttttc
     1021 cgggtgtatt cggcataacc cggacaatgc ggcgtgttcc cccgaggtaa cggctgagcg
     1081 taccgaccga caatccggct gcgacagaaa gcaccaatgc gccgttggtg cggatatttt
     1141 tgcacgcagc ttccatatcc tgcggtttga cggcaaggat taaaacatcg tcggaatgaa
     1201 gctccggcag ggttgccgaa gtttcgaccc ccaactcttt ttccaaacgt tcgcgttttt
     1261 ccgcaccccg attggctata tagatgcggt aaccgccttg tttgaccaat ccgcccgcaa
     1321 cggcagccgc catattgccg ccgccgagaa aataaacatt cattatttct accctattca
     1381 aaattacctt actaaattca ttcccaattt tttaccatcg gacgcgctcg gacagaccgt
     1441 tgcgccgcaa cccctgcttt tccaagcagt caaatcctcc ctgtttgaac gtctcaatca
     1501 aaggtttgcc gtcacgcgta cgccaatcca gcccgcaggc ttcaaacttc cctattttcc
     1561 tgtaaaagta agattgttgt gcggattcat attcgtttgc cctgacggaa cgattgccgt
     1621 ctgaaagcgg tatctcgtag tcccctaaag acggcggact ctcgccgcca atatcccaaa
     1681 aatcgatggc agccggtttt ctcggcttac accagccggt gagggacgaa cactcatacc
     1741 accccgccag ccggcagcct gcaaccgtaa atgcaagcaa cgcagccatt gccgtatatt
     1801 ttattgcccg aatttccatc agcccctttt cccgaaaatc gcgctgccga tacggacgtg
     1861 tgtcgcaccg cactcaatgg cggcaggcat atcgtccgac atccccatag acagcacgtc
     1921 tgccttaacg ccagccgcat tgaggtcggc aagcagtttc cgcatcgttt gaaattgcac
     1981 cttcaactcc gtttcactgc tgttggcttt ggcaacacac atcagtccac gtacgacgat
     2041 attcggcagc ttcgccactt ccacagcaag cgcgactgct tcttcgggcg cgacaccgtg
     2101 cttcaccgcc tcgcccgcaa tgttcacctc gatacacacc tgcaaaggcg gcattgagga
     2161 aggacgttgc ccgctcagcc ggacggcggt tttcagacgg catacggtat gcacccaatg
     2221 cgcgcgttcg gcgacaaact tggttttgtt ggactgcaca tcgccgatga cgtgccacac
     2281 gatgtcggtc aaatccgcca actcttccgt tttgccgtac cactcctgaa tatagttctc
     2341 gccgaaatca cgctgtccgg cggcgtaaac ttcgcggatg ccgtctgaag ggaaagtctt
     2401 accgacggca atcaggctga cggaatgcgg ctccctgccc gcctgcagaa ccaattttcc
     2461 gatacggtcg gacacctcac aataacgttc ttgcaacacc gtcatagatt atcccctaat
     2521 taaaaatgat taaacagttg aaacccctcc agtcagggcg gtacaatcaa ggttgttaga
     2581 accatttcaa ccaatcgaaa cattatacta aacagagccg cattatgcag attaccgact
     2641 tactcgcctt cggcgctaaa aacaaagcat ccgaccttca cctgagttcg ggcatatccc
     2701 ctatgattcg ggttcacggc gatatgcggc gcatcaacct tcccgaaatg agcgcggaag
     2761 aggtcggtaa tatggtaact tcggtgatga acgaccacca gcggaaaatc taccagcaaa
     2821 acttggaagt cgacttctcg ttcgaactgc ccaacgtcgc ccgattccgc gtcaacgcct
     2881 tcaacatcgg ccgcggtccc gccgccgtat tccgcaccat tcccagcacc gtcttatcgc
     2941 tggaagaatt gaaagccccg agcattttcc aaaaaatcgc agaatcgccg cgcggcatgg
     3001 ttttggttac cggccctacc ggttcgggca aatcgaccac gcttgccgcg atgatcaact
     3061 acatcaacga aacccagccg gcacacatcc tgaccatcga agacccgatt gaattcgtcc
     3121 accaaagcaa aaaatccctg attaaccagc gcgagctgca ccagcacacc ctcagcttcg
     3181 ccaacgcgct gcgttccgca ttgcgcgaag accccgacgt tatccttgtc ggcgagatgc
     3241 gcgacccaga aaccatcggc ttggcactga ccgccgccga aaccggacac ttggttttcg
     3301 gcacgctgca cacgaccggc gcagcaaaaa ccgtcgaccg tattgtggac gtattcccgg
     3361 cgggagaaaa agaaatggtg cgctctatgc tgtccgaatc gctgaccgcc gtcatctccc
     3421 aaaacctgct gaaaacgcac gacggcaacg gccgtgtcgc ctcgcacgaa atcctgattg
     3481 ccaaccccgc cgtccgcaac ctcatccgcg aaaacaaaat cacgcagatt aactccgtcc
     3541 tgcaaaccgg gcaggcgagc ggtatgcaga caatggacca atcgctgcaa tcgctggtgc
     3601 gccaagggct gatcgcaccg gaagtcgcac gcagacgcgc gcaaaacagc gaaagtatga
     3661 gtttctgaca cacaaccgct ttccggccat accggcggga aaacaaggcg caaacacgcg
     3721 gggcgggacg cagcatcccg cccggctacc tttccgaaca agaagcgtcc gccttcctgt
     3781 tgaaacctgc cgccgcaaac tgcaaggctt aaaccgaaaa gaagctaacg atgaataccg
     3841 ataacctgca cgacatcttg gacgaaatgg ttcaagtgta ttctcaaaaa aaacaaagcc
     3901 gatccgaaac cccggccgaa atcggcgcac acttccaccc gctgctcgac cgcctgtgcg
     3961 aaaccgcaga agcacaaaac gcgtccgaca tccttatcag caaaggattc ccgccctcgt
     4021 tgaaaatcaa cagcgcatta accccgcagc cgcaaaaggc gctgacgggc gaggaaaccg
     4081 ccgccatcgc cgcatcgacg atgaacgccg aacaatcgga aatattccgg cgcgacggcg
     4141 aaatcaacta ctccgtccag tcgcgcagcg gcacgcgcta ccgcgccaac gcctaccaca
     4201 gccaaggcag cgcaggtttg gttttgcggc gcatcaacca cgtcatcccg caaatgcagg
     4261 aattgggcct gcccgaaaaa ctcaaagacc tcgccgtcgc accgcgcggg ctgctgatta
     4321 tcgtcgggcc taccggttcg ggcaaatcca ccacgatggc gactatgctc gaacaccgca
     4381 acaaaaccct gcccagccat atcgttacca tcgaagaccc gattgaattt atctacaaac
     4441 cgcgccgctg catctttacc cagcgcgaaa tcggcgtcga caccataaac tggcagacgg
     4501 cggtacaaaa cgctatgcgc caatcccccg acgtggtctg catcggcgaa gtccgcagca
     4561 gggaaagtat ggaatacgcg atgcagctcg cccaaaccgg ccacctgtgc atttttacgc
     4621 tccacgccaa caccgcgccg cagtcgctcg aacgcatact caacttctac cccaaagaac
     4681 agcacaacca aatactgatc gacatcgccc tcaacctgac cggcatcatc tgccaacgcc
     4741 tcgccctcaa acaagacaaa acgggcagga cggcggttgt cgacttgctc atcaacacgc
     4801 ccgccatcca agacttcatc ctgaagggcg acctgatgaa catcagtaaa atcatggaaa
     4861 ccgccaaaac cgacggaatg cagacgatgg atcaaaacct tttcgaactg taccgtcacg
     4921 gcatcatcag ttacgaagaa gccctgcgcc agtccgtttc cgccaacaac ctgcgattgc
     4981 acatccaact gcacaaagaa ggcaaaacgc ccgaactcct ttacgacagg gtcaacggtc
     5041 tcaacctcat ttcctgatcc gcaaaaccca atgccgtctg aaaaccgcat ccccgttttc
     5101 agacggcatg attttatccg ccccattcat gtgctacact ttatagtgga ttaaatttat
     5161 agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagcacaa atagtacgga
     5221 accgattcac ttggtgcttg agcaccttag agaatcgttc tctttcagct aaggcgaggc
     5281 aacgccgtac tggtttttgt taatccacta tattcacttc ataacaataa accggtaaaa
     5341 ccatgaaaac cccactcctc aagcctctgc tcattacctc gcttcccgtt ttcgccagtg
     5401 tttttaccgc cgcctccatc gtctggcagc taggcgaacc caagctcgcc atgcccttcg
     5461 tactcggcat catcgccggc ggccttgtcg atttggacaa ccgcctgacc ggacggctga
     5521 aaaacatcat caccaccgtc gccctgttca ccctctcctc gctcacggca caaagcaccc
     5581 tcggcacagg gctgcccttc atcctcgcca tgaccctgat gaccttcggc ttcaccattt
     5641 taggcgcggt cgggctcaaa taccgcacct tcgccttcgg tgcactcgcc gtcgccacct
     5701 acaccacact tacctacacc cccgaaacct actggctgac caaccccttc atgattttat
     5761 gcggcaccgt actgtacagc accgccatcc tcctgttcca aatcgtcctg ccccaccgcc
     5821 ccgtccaaga aagcgtcgcc aacgcctacg acgcactcgg cggctacctc gaagccaaag
     5881 ccgacttctt cgaccccgat gaggcagcct ggataggcaa ccgccacatc gacctcgcca
     5941 tgagcaacac cggcgtcatc accgccttca accaatgccg ttccgccctg ttttaccgcc
     6001 ttcgcggcaa acaccgccac ccgcgcaccg ccaaaatgct gcgttactac tttgccgccc
     6061 aagacataca cgaacgcatc agctccgccc acgtcgatta tcaggaaatg tccgaaaaat
     6121 tcaaaaacac cgacatcatc ttccgcatcc accgcctgct cgaaatgcag ggacaagcct
     6181 gccgcaacac cgcccaagcc ctgcgcgcaa gcaaagacta cgtttacagc aaacgcctcg
     6241 gccgcgccat cgaaggctgc cgccaatcgc tgcgcctcct ttcagacagc aacgacagtc
     6301 ccgacatccg ccacctgcgc cgccttctcg acaacctcgg cagcgtcgac cagcagttcc
     6361 gccaactcca gcacaacggc ctgcaggcag aaaacgaccg catgggcgac acccgcatcg
     6421 ccgccctcga aaccagcagc ctcaaaaaca cctggcaggc aatccgtccg cagctaaacc
     6481 tcgaatcagg cgtattccgc catgccgtcc gcctgtccct cgtcgttgcc gccgcctgca
     6541 ccatcgtcga agccctcaac ctcaacctcg gctactggat actactgacc gcccttttcg
     6601 tctgccaacc caactacacc gccaccaaaa gccgcgtccg ccagcgcatc gccggcaccg
     6661 tactcggcgt aatcgtcggc tcgctcgtcc cctacttcac cccgtctgtc gaaaccaaac
     6721 tctggattgt catcgccagt accaccctct ttttcatgac ccgcacctac aaatacagtt
     6781 tctccacctt cttcattacc attcaagccc tgaccagcct ctccctcgca ggtttggacg
     6841 tatacgccgc catgcccgta cgcatcatcg acaccattat cggcgcatcc cttgcctggg
     6901 cggcagtcag ctacctgtgg ccagactgga aatacctcac gctcgaacgc accgccgccc
     6961 ttgccgtatg cagcaacggt gcctatctcg aaaaaatcac cgaacgcctc aaaagcggcg
     7021 aaaccggcga cgacgtcgaa taccgcgcca cccgccgccg cgcccacgaa cacaccgccg
     7081 ccctcagcag caccctttcc gacatgagca gcgaacccgc aaaattcgcc gacagcctgc
     7141 aacccggctt taccctgctc aaaaccggct acgccctgac cggctacatc tccgccctcg
     7201 gcgcataccg cagcgaaatg cacgaagaat gcagccccga ctttaccgca cagttccacc
     7261 tcgccgccga acacaccgcc cacatcttcc aacacctgcc cgaaaccgaa cccgacgact
     7321 ttcagacagc actggataca ctgcgcggcg aactcgacac cctccgcacc cacagcagcg
     7381 gaacacaaag ccacatcctc ctccaacagc tccaactcat cgcccgacag ctcgaaccct
     7441 actaccgcgc ctaccgccaa attccgcaca ggcagcccca aaatgcagcc tgaaaaagtt
     7501 tcggcatttt gtaagagagg acagattgtc agacaggtta caagatagtg gataaagctt
     7561 tgcgcagggt aaatgcgtag caactgaacc gtcattccca cgaacctaca tcccgtcatt
     7621 cccacgaaaa cagaaaacca aaaacagaaa cctaaaatcc cgtcatttcc acgaca
//
LOCUS       DAWGRZ010000095         7660 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000095 DAWGRZ010000000
VERSION     DAWGRZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.095"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            318..452
                     /locus_tag="WHT44_001369"
                     /pseudo
     CDS             318..452
                     /locus_tag="WHT44_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
     gene            407..622
                     /locus_tag="WHT44_001370"
     CDS             407..622
                     /locus_tag="WHT44_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ2533547.1"
                     /translation="MFLFCIAMLVIGIPMILVENVIGRRKGVNALDAFGGPMNGKPIA
                     KIWKLVGWMGCSARSASWLITWYSAAG"
     gene            825..1633
                     /locus_tag="WHT44_001371"
                     /pseudo
     CDS             825..1633
                     /locus_tag="WHT44_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            1877..2533
                     /locus_tag="WHT44_001372"
     CDS             1877..2533
                     /locus_tag="WHT44_001372"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ2533548.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYREV
                     "
     gene            2689..4374
                     /gene="rpsA"
                     /locus_tag="WHT44_001373"
     CDS             2689..4374
                     /gene="rpsA"
                     /locus_tag="WHT44_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ2533549.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGDILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            4385..4699
                     /locus_tag="WHT44_001374"
     CDS             4385..4699
                     /locus_tag="WHT44_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ2533550.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(5050..5457)
                     /locus_tag="WHT44_001375"
     CDS             complement(5050..5457)
                     /locus_tag="WHT44_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ2533551.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            5605..6714
                     /locus_tag="WHT44_001376"
     CDS             5605..6714
                     /locus_tag="WHT44_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ2533552.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFDLMHEGKSIRAVIHY"
     gene            6723..7550
                     /gene="fghA"
                     /locus_tag="WHT44_001377"
     CDS             6723..7550
                     /gene="fghA"
                     /locus_tag="WHT44_001377"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ2533553.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVAPDTSPRGEQVPNDDAYDLGQSAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
BASE COUNT         2043 a         1866 c         1837 g         1914 t
ORIGIN      
        1 aattccctct ctttcatcat tatttaaatg ctatcattaa tataattaac aaaatttcat
       61 ttccatacac cgatgtagaa gaataacaaa ttaatctatt tataaactcc gattcttcct
      121 catcgggtta tactcatcag aaatactcac tgaaatatat gccttttaca tggaaaatca
      181 ctagacagtt gaaaagcaat cagtcagttc ctgttaaaaa acgcgtgtag aatttccgct
      241 ttatctcaat cggacacggt ttcaatatgt cttccccttc aaataccaat cgtcaaacct
      301 ggtccagccg attaacctat atcctgaccg ttgccggcgc gactgtcggt ttcggcgcga
      361 cgtggcgttt cccgtatttg gtcggtgaaa acggtggtgc gcgtatgtgt ttttattctg
      421 tatcgcgatg ctggttatcg gcatcccgat gattttggtg gaaaatgtca tcggacggcg
      481 caaaggcgtg aacgcgctgg atgcgttcgg cggcccgatg aacggcaaac ccattgccaa
      541 aatttggaaa ctggtcggct ggatgggctg ctcggcgcgt tcggcatcat ggcttattac
      601 atggtactcg gcggctgggt aatcagctat atcgttaata ttattggagg aaatttgaat
      661 atttccagcc ccgtcgacgg tgtggttaca aaaggcttct ttgccgaaca cattgaaaca
      721 gcccttggga aattgcgttt tatacgctgc tttttgtcgc cgtgaaccaa tggattttgg
      781 tcaaaggcgt tatcggcggc attgaaaaag cggcaaaata gctgatgccg ctgctgtttt
      841 tgttcctaat cgcgatggtc gtccgcaacg ttacccttcc gggcgcaatg gaaggggttg
      901 ctttctatct gaaacctgat ttcagcaaga ttaccgccga actgttcgtc ttcgttttgg
      961 ggcaggtatt ttttgccctg agcttgggtt tcggcgtgat gattaccttg tccagctatt
     1021 tggataaaaa cgaaaatctg gttcagacgg cagttatcac ggcaattacc aataccatca
     1081 tcgccatact tgcgggcttt atgattttcc cgtcgctctt cagcttcggc gttgcccccg
     1141 attccggccc gactttggtg ttccaaagct tgccgattgt gttctcacat atgtgggcgg
     1201 gatctgtgtt cgccgtgatt ttcttctcgc tgctcctgat tgccgcgttg acaacttcgc
     1261 tgaccattta tgaagtgttg attacgacca ttcaggaaaa aaccaaaatc cgccgtaccg
     1321 ccgcgattac gattgtattg gctgccatct tcattttcgg caacatcccg tccattctga
     1381 gctatggtcc gtggaaagac gttccgtgtt cggcaaaaat attttcgatg ccttcgacta
     1441 catcagcggc aacatcttgt ttatgctgac cgcgctcggt tccgcgctgt ttgccggttt
     1501 tgtgatgaag gacgaagcga aggacgaatt gctttataaa ggcaaccata cgacggtcaa
     1561 tatttggttt gcttatgtga aatatcttgt gccgctggtg attctgctga ttttcgtcag
     1621 caacctgttc taatccgcag caatcgatgc cgtctgaagg tcatacctct ttcagacagc
     1681 attgccttga tgcccgccac aatacccgca acgccgaaat ccgaaccggc ttgccggctt
     1741 ctgtcggatg tttccgggca ggcggcgttc cgtctgctta gacaatcgtc ctttaaaaca
     1801 ggtagaatcc gccccaacgg gaaacacatc cttcagacgg caaaacccat accccaaacc
     1861 atcaggaatc ccccttatga acagacaaaa agtcatcgcc atcgacggcc cgggcgcatc
     1921 gggcaaaggc acggttgccg cccgcgttgc cgccgcattg ggatacgatt atctcgatac
     1981 cggcgcactc taccgcctga ctgccctata tgcacaaaaa caaggcgtgg gatggcacga
     2041 tgaagaaaac gtttccgaac tggcaaaaaa actgcccgcc gtattttcag gcagccgcat
     2101 cctgctcggc ggcgaagacg tttcagacgg catccggaca gaagccatcg gcatgggcgc
     2161 atccgcagtc gcacagttgc ctaaagtccg cgccgccctg ctgcaacgcc aacgcgattt
     2221 tctgaccgaa aaaggactgg ttgccgacgg acgggacacc ggatcggtcg tcttccccca
     2281 agccgaactt aaaatcttcc tgacggcaga atccaaaatc cgtgccgaac gccgcgccaa
     2341 acaaatcggc atcccctgcg aaggtttggc attcgagcgc atcctgtccg acatcgaagc
     2401 cagagacgag gcagaccgaa accgcaaagt tgcccccctg aaacaacagc ccgatgccct
     2461 gcttttggac acaagccgcc tgactataga agaaactgta aaaaaagtgc ttgattggta
     2521 tcgtgaagtt taaattttca ggtataatcg cacaaattac gtttcagacg gcataaaaat
     2581 cccccatatg ccgtctgaaa ccttatgtac ccgtctgccc tgccaaggac ggcagatatc
     2641 caccaaccca ccccgcaccc cttggcggtg taccgaaaag agttatatat gtctatggaa
     2701 aattttgctc agctgttgga agaaagcttt accctgcaag aaatgaaccc gggtgaggtg
     2761 attaccgctg aagtagtggc aatcgaccaa aacttcgtta ccgtaaacgc aggtctgaaa
     2821 tcagaatccc tgattgatgt agctgaattc aaaaacgctc aaggcgaaat tgaagttaaa
     2881 gtcggcgact tcgttaccgt taccatcgaa tccgtcgaaa acggcttcgg cgaaaccaaa
     2941 ctgtcccgcg aaaaagccaa acgtgcagcc gattggattg ccctggaaga agccatggaa
     3001 aacggcgaca tcctgtccgg catcatcaac ggaaaagtca aaggcggcct gaccgttatg
     3061 attagcagca tccgcgcatt cctgccgggt tctttggtcg acgtacgtcc tgtaaaagac
     3121 acttctcact tcgaaggcaa agagatcgaa ttcaaagtga tcaaactgga caaaaaacgc
     3181 aacaacgtcg ttgtttcccg ccgcgccgtt ctggaagcca ctttgggtga agaacgcaaa
     3241 gccctgctgg aaaacctgca agaaggctcc gtcatcaaag gcatcgttaa aaacattacc
     3301 gattacggtg cattcgttga cttgggcggc atcgacggtc tgttgcacat caccgatttg
     3361 gcatggcggc gcgtgaaaca cccgagtgaa gtcttggaag tcggtcagga agttgaagcc
     3421 aaagtattga aattcgacca agaaaaacaa cgcgtttcct tgggtatgaa acaactgggc
     3481 gaagatcctt ggagcggtct gacccgccgt tatcctcaag gcacccgcct gttcggcaaa
     3541 gtatccaacc tgaccgacta cggcgcattc gtcgaaatcg aacaaggcat cgaaggtttg
     3601 gtacacgtct ccgaaatgga ctggaccaac aaaaacgtac acccgagcaa agtcgtacaa
     3661 ctgggcgacg aagtcgaagt catgattttg gaaatcgacg aaggccgccg ccgtatctct
     3721 ttgggtatga aacaatgcca agccaatcct tgggaagaat ttgccgccaa ccacaacaaa
     3781 ggcgacaaaa tctccggcgc ggttaaatcc attaccgatt tcggcgtatt cgtcggcctg
     3841 cccggcggca tcgacggttt ggttcacctg tccgacctgt cctggaccga atccggcgaa
     3901 gaagccgtac gcaaatacaa aaaaggcgaa gaagtcgaag ccgtcgtatt ggcaatcgac
     3961 gtggaaaaag aacgcatctc cttgggtatc aaacaactgg aaggcgatcc gttcggcaac
     4021 ttcatcagcg tgaacgacaa aggttctttg gttaaaggtt ccgtgaaatc tgttgacgcc
     4081 aaaggtgctg ttatcgccct gtctgacgaa gtagaaggct acctgcctgc ttccgaattt
     4141 gcagccgacc gcgttgaaga tttgaccacc aaactgaaag aaggcgacga agttgaagcc
     4201 gtcatcgtta ccgttgaccg caaaaaccgc agcatcaaac tttccgttaa agccaaagat
     4261 gccaaagaaa gccgcgaagc actgaactcc gtcaatgccg ccgccaatgc gaatgccggc
     4321 accaccagct tgggcgacct gctgaaagcc aaactctccg gcgaacaaga ataaggttgc
     4381 agacatgaca aagtctgagt taatggttcg tttggcagaa gtgtttgccg ccaaaaacgg
     4441 cacgcatctt ctggcaaaag acgtagagta cagcgtaaaa gtcttggttg acaccatgac
     4501 tagatcgctt gcccgaggtc aacgcatcga aatccgcggt ttcggcagct tcgatttgaa
     4561 ccatcgtcct gcccgcatcg gtcgcaatcc caaaaccggc gagcgtgtgg aagtacctga
     4621 aaaacatgta ccccacttca agcccggtaa agaattgcgc gagcgggtcg acttggcttt
     4681 aaaagaaaat gccaattaaa ccttagcatc aaaacgccgc tgttacgcgg cgttttttct
     4741 gtggtttaac ttcatccgtt gcttcaatac cttgagccaa gcaagcaacg gattagagcg
     4801 tggatttttt tatagtggat taacaaaaac cagtacggcg ttgcctcgcc ttagctcaaa
     4861 gagaacaatt ctctaaggtg ctgaagcacc aagtgaatcg gttccgtact atttacactg
     4921 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatatcatt gcttacaatc
     4981 cgctttttaa acaacaaatt tttgatttct attacgaaca ggacaaaaat cctgcttatt
     5041 gcactaaaac taagccgttt caggaatttg cggcaaattt acagctttta ccgagcctaa
     5101 tgctttcgct ttttggtaaa acgccaattt gtattcaagc aaatctaaat agcgttttaa
     5161 ttcggcaatt tgacacttca cattttctat ttgattttca aacaaggaaa ggcgttcttc
     5221 aatggtatcg tcgccaatga cggtacattc cgcaaagcgt ttgatgtctt ttaagctcat
     5281 tcccgtattt ttcaagcatt gcaataagcc caaccattgc aaatcgttat cggtaaaaca
     5341 gcggttaccg tattcatcac gtccgatatt gggcaacaaa ccttctttgt cgtaaaaacg
     5401 tagggtgtgg gcggagatgc ctattttttc ggcggctttg gcagtagtat aagtcatttt
     5461 ccctccttct aaacaaaaac agtaaaaaac acttgcttta gagttaactc taaagtgtaa
     5521 actgttgcta tgttgctcag gcaaggcaac tttgtcaatg aattaagagg aaagacaatg
     5581 gaaatgaaac aagccgattc aaccatcaaa tctcgtgcgg cggtggcatt cgcccctaac
     5641 caacccttac aaattgtgga aatcgacgta gaaatgccgc gtaaaggcga ggtgttaatc
     5701 cgcaataccc acactggcgt gtgccatact gatgcgttta cgttatcagg aagcgatcct
     5761 gaaggcgtat tccctgtggt gcttggacac gaaggtgcgg gtgtggtcgt tgctgtgggc
     5821 gaaggtgtgt caagcgtaaa accgggtgat cacgtgattc cgctttacac cgccgaatgt
     5881 ggcgaatgtg agttttgttg ttcaggtaaa accaacttgt gcgtctcagt gcgtgataca
     5941 caaggtaaag gcttaatgcc ggacggcacg acgcgttttt cttatcaagg tcagccaatc
     6001 tatcactata tgggctgttc gactttcagt gaatactccg ttgttgccga agtttcactg
     6061 gcgaaaatca accctgaagc caaccatgaa caagtatgtt tgctcggctg cggcgttacc
     6121 acaggtattg gtgcggtaca taatacggca aaagtgcaag aaggcgactc tgttgccgtg
     6181 tttggtttgg gggcgattgg tttggcggtg gtgcaaggtg cgcgtcaagc caaagccggc
     6241 cgcattatcg ccattgatac caatccatca aaatttgagt tggcaaaaca gttcggtgca
     6301 acggattgtt tgaacccgaa cgattacgat aaaccgatca aagatgtgtt gttagacatt
     6361 aataaatggg gcattgacca tacctttgaa tgtatcggca atgtaaacgt aatgcgtcag
     6421 gcattagaaa gtgcacatcg tggttggggt caatccatta tcatcggcgt agcaggtgca
     6481 ggacaagaaa tttcaacgcg tccgttccag ttggtaacag gtcgtgtttg gaaaggttca
     6541 gcatttggcg gtgtgaaagg tcgctctgaa ctgccgaaaa tggtggaaga ttcaatgaaa
     6601 ggcgacattg agttagaacc gtttgtaacc cacacaatga cactcgatca aatcaataaa
     6661 gcctttgact taatgcacga aggtaaatcg atccgcgccg ttattcacta ctaaggtatg
     6721 cgatgaaact gattgaacaa catcaaattt ttggtggttc gcaacaagtt tgggcgcatc
     6781 atgcccaaac gctgcaatgc gaaatgaaat ttgccgtcta tttgccaaat aatccagaaa
     6841 atcgaccgct tggtgtgatt tattggcttt ccggcttgac gtgtacggaa caaaatttca
     6901 ttaccaaatc aggctttcag cgttatgcgg cagaacatca agtaattgtg gtggcccccg
     6961 ataccagccc tcgcggagag caagtgccga acgatgatgc ttacgattta ggacagagtg
     7021 caggctttta tttgaatgcg accgaacagc cttgggcggc gaattatcaa atgtatgatt
     7081 acattttgaa cgagctgccc cgtctgattg agaaacactt tcctaccaac ggcaaacgtt
     7141 ccattatggg acattcaatg ggcggacacg gcgcattggt attggcgctg cggaatcagg
     7201 aacgttatca aagtgtttct gccttttcgc ctattttatc gccaagcctc gtgccgtggg
     7261 gagaaaaagc ctttactgct tatttaggga aagaccgtga aaaatggcag caatatgatg
     7321 ctaactcact cattcaacaa ggctataaag tgcaaggtat gcgcatcgat caaggcttgg
     7381 aagatgagtt tttgccgaca caattgcgta ccgaagattt tatcgaaacc tgccgtgcgg
     7441 caaaccagcc ggtcgatgtg cgtttccata aaggctacga tcacagctat tacttcatcg
     7501 ccagttttat tggcgagcat attgcttatc acgccgcgtt tttgaagtaa accaaagagc
     7561 gttcagtgtt caaagcagtt ttgggatagc cggcacgagg gcggtaagaa gtgccggcat
     7621 aaacgtatgc cgtctgagcc gaaaggagcc gactctacgg
//
LOCUS       DAWGRZ010000096         7648 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000096 DAWGRZ010000000
VERSION     DAWGRZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.096"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(30..1232)
                     /gene="trpB"
                     /locus_tag="WHT44_001378"
     CDS             complement(30..1232)
                     /gene="trpB"
                     /locus_tag="WHT44_001378"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ2533554.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALQELADAYKAAKNDPE
                     FWEAFRHDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(1315..2004)
                     /locus_tag="WHT44_001379"
     CDS             complement(1315..2004)
                     /locus_tag="WHT44_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ2533555.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDPVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLHERGIASCPTVLKAVRQHGNASDKFLKVVETVDLAAVLAKIPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
     gene            2201..2980
                     /gene="rsmA"
                     /locus_tag="WHT44_001380"
     CDS             2201..2980
                     /gene="rsmA"
                     /locus_tag="WHT44_001380"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ2533556.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDIVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKVV"
     gene            3153..3881
                     /locus_tag="WHT44_001381"
     CDS             3153..3881
                     /locus_tag="WHT44_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ2533557.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQGFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            3954..4313
                     /locus_tag="WHT44_001382"
     CDS             3954..4313
                     /locus_tag="WHT44_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533558.1"
                     /translation="MYFVDRTAVVLKPTARFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     NMEEAELNITPVFDNMM"
     gene            4318..4764
                     /locus_tag="WHT44_001383"
     CDS             4318..4764
                     /locus_tag="WHT44_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ2533559.1"
                     /translation="MRAFQTALRPSRILNILTVSLHAASLAVCLTWFYGRMMWFGLAA
                     LVASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKDKTAKAAALSGSSMVTPYALF
                     LQWDTGGKTVRQCIMPDMTDKESYRKLKVWVLWRQPKKTAETDTSD"
     gene            4795..6069
                     /gene="folC"
                     /locus_tag="WHT44_001384"
     CDS             4795..6069
                     /gene="folC"
                     /locus_tag="WHT44_001384"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ2533560.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERTSEVKKRMKLEPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKIGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNALAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPKSLLSHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDDPARNRNALPFPALRGTYQLSNAACALTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLETVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            6082..7080
                     /gene="ftsN"
                     /locus_tag="WHT44_001385"
     CDS             6082..7080
                     /gene="ftsN"
                     /locus_tag="WHT44_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ2533561.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINERLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            7073..7570
                     /locus_tag="WHT44_001386"
     CDS             7073..7570
                     /locus_tag="WHT44_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ2533562.1"
                     /translation="MNSLPIADLLVSAVIAACIVLSAMRGVIAEAGSMAAWVVSFFFA
                     KLFAASFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTSAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
BASE COUNT         1932 a         2165 c         1973 g         1578 t
ORIGIN      
        1 tgttaatcca ctatatcaga cggacgaggt tacagtttaa tccctttgag cttcgcgacg
       61 gtattgatgt ctttgtcgcc acgacctgag aggttgacca aaatcacttg gtctttaccc
      121 attttcggcg catttttcac cgcccaagca acggcgtggc tggattcgag cgcggggatg
      181 atgccctcga agcggcagag caagtcaaag gcttcgagtg cttcgtcgtc tttggcaaca
      241 gtgtattcga cgcgcttgat gtcgtgcaga tggctgtgtt ccgggccgat gccggggtaa
      301 tccaagcctg cggaaacaga gtgcgtaccc aaaacctgac cgttttcgtc ctgcatcaga
      361 tagctgcgga aaccgtgcaa tacgccaatc ggtgcgcccg aagtaatcgg cgcggcgtga
      421 tcgggggtgt tcacgcccaa accgccagcc tccacgccga cgaggcgcac gttttcttcg
      481 ccgatatacg ggtggaacaa accgatggcg ttcgatccgc cgcccacgca ggcaacggca
      541 acgtcgggct gtctgccgat ggcttcctgc atctgcgctt tagcttcgtt gccaatcacg
      601 cattggaaat cgcgcaccat ttcgggatac ggcgcggggc cggcggcggt accgatgatg
      661 tagaacgtgt cgtccacgcg ggcgacccat tcgcgcatgg cttcgttcat cgcgtctttc
      721 agcgtgcggc tgccgctttc tacaccgacc acgttcgcac ccaataattt catacggaac
      781 acgttgggca tttggcgttg gatgtcgtcc gcgcccatat acacgtcgca agtcataccg
      841 aagcgtgcgg caacggtggc actcgccacg ccgtgctgac ccgcgccggt ttcggcgatg
      901 acgcgttttt tacccatgcg ttttgccaac agtgcctgac cgatggtgtt gttgactttg
      961 tgcgcgccgg tgtggttcaa gtcttcgcgc ttcaaccaga tttgcgcgcc gcccagatgt
     1021 tcggacaacc gcgcggcgtg gtaaacgggg ctgggcctgc cgacataatg tttcaaatca
     1081 tggcggaacg cttcccaaaa ttcaggatcg tttttcgctg ccttataggc atccgccagc
     1141 tcttgcaagg cgggaatcag ggtttcggag acataaagcc cgccgtgttc gccgaaaaag
     1201 cccttctcgt cgggcgcgtg gtagttcttc atcggattct tccttgtgct tcaaattttc
     1261 agacggcatt tccgtttttg caaacatgcc gtctgaacgg gaaattgtcg gcaattataa
     1321 ctgtttttca cacaagcccg ccatttcaaa aaacgcccga tacgccgccg cttttttcgc
     1381 caaactcaaa ggataggcgc gcgaagtcga aggcaggcgc gtcagcgtca aatcccgtcc
     1441 ggcaaacgga aacggcacgg tttcgcccgt tttcggcatt ttgataccgc cgccctgaat
     1501 atcgagcagg atttccgtcg ccttgccgcc tgtcgtacaa atatggcggc actcgggtat
     1561 ttttgccaac accgccgcca aatcgacggt ttcaactacc tttaaaaact tgtcggacgc
     1621 attgccgtgc tgacgtaccg ccttcaaaac ggtcggacag gacgcaatcc cccgttcgtg
     1681 caaaaacgcc ttgattttct cggcatcaaa cgctttttca gacaaccttt ggaaatgcgc
     1741 cgcatcatta aaaaacacca gcccataaac gcgccacata tcgttttgga aattcggata
     1801 atgaaactgc atcgcgcgtt tgtcttcctt gggcggaaac gtccccatca tcatgacagc
     1861 agccttcggc ggcaacacgg gatcgaaggg atgggtttcg atttcaagca gattttcaga
     1921 cacgttcaat cctccaacag gcaaaacggc agacagccgg tttttgcacc aaaccgccaa
     1981 acacggcaag ccttcaaaca gcattatcac gccctgccgt tttcggttac aatgccgaca
     2041 tcaaacggat actgtaaaca catttatgtt tcaacacaca ggacgacaca taaagcaccg
     2101 ccctatgtgc catcctgatt tggaaagagt tacgcccctc ccaaatagag tctgattcta
     2161 ccgccctaaa gggcggggtt tcaaccgaaa aggaaacacg atgaaagaac acaaagcccg
     2221 caagcgtttc gggcagaatt ttttgcagga cacgcggatt atcagcgata ttgtcaacgc
     2281 cgtgcgcccg caggcggatg atatcgtgat tgaaatcggc ccgggtttgg cggcgattac
     2341 cgaacctttg gcgaaaaagc tgaaccgcct gcacgttgtc gaaatcgacc gcgacatcgt
     2401 atgccgtctg aaaacgctgc cgtttgcgga taaactggtg attcacgaag gcgatgtatt
     2461 gcagtttgat ttcaacggca tcgcaggcaa aaagaaaatc gtcggcaacc tcccgtacaa
     2521 catttccaca ccgcttttgt tcaagctggc ggaggtggcg gacgatgtcg tcgatatgca
     2581 ctttatgctg caaaaagaag tggtcgagcg tatggttgcc gcgccgaaaa gcaacgacta
     2641 cggccgcttg ggcgtgatgc tgcaatattt tttcgatatg gaaatgctga ttgacgtacc
     2701 gcccgaatcg ttcgaccctg cgccgaaagt ggattccgcc gtcgtccgta tgattccggt
     2761 gaaacaccgc atcggcaagg cggacgattt cgagcatttt gccaaactcg tgaaactcgc
     2821 cttccaccaa cgccgcaaaa ccatacgcaa caatctgaaa gagcttgcag gcgacgatga
     2881 tttgcaggca gtcggcatca atccgcaaga ccgcgccgaa catatcgcgc cagagaaata
     2941 cgtggcgttg agcaattatc tggcgggcaa ggtcgtctga aaagggagta agccgatgac
     3001 gaaatacctg cttttacccc tgatactgcc gcttgcagcc tgcctgacca atccgaacga
     3061 ccgttttttt gacggcagac gctacacgct tcatcatatg gagctcccgt aacgacaacc
     3121 tgcaacggca aatgccgtct gaaagaaaag acatgatcaa attcaaaaac gtacacaaac
     3181 atttcaaaga cctgcacgtc atcaacggcg taaacttgga aatcaaaaaa ggcgaagtgg
     3241 tcgtcgtctg cgggccttcg ggcagcggca aatccacact catccgcact gtcaaccagc
     3301 ttgaaagcat cgaaagcggc gagatttggg tggacggtgt caacgttgcc gaccccaaaa
     3361 ccgatttgaa caaaatccgc gaagaagtcg gcttcgtatt ccaaggcttc aacctctacc
     3421 cgcacctgac cgtgttggaa aacattaccc tcgcaccgat gaaggttaag gggcaaaatg
     3481 cggaacaggc ggagaaaaag gcaatggagc ttttggaacg cgtcggactg gcacacaaaa
     3541 aagacgcctt cccttcccaa ctttccggcg gccagcagca acgcgtggcg attgcacgcg
     3601 gtttggcgat ggaaccgcgc gtaatgttgt ttgacgaacc gacctccgca ctcgaccccg
     3661 aaatggtcgg cgaagtgttg aaagtgatga aagacttggc agaaagcggc atgacgatga
     3721 tgtgcgtaac ccacgaaatg gggtttgccc gcgaagttgc cgaccgcgtg attttcgtcg
     3781 ataaagggca aatcctcgaa gacgaaacgc ccgaagcgtt ttttaccaac ccgaaacacg
     3841 aacgcgccaa gcagttcctg caacaggtca tgacccactg atgctatacc gtctgaaaac
     3901 cgaacgttca gacagcatat cccgacaccg caagatgatt gagaaagaaa accatgtatt
     3961 ttgttgaccg caccgccgtc gtcctcaagc cgaccgcgcg ctttttggaa tggctcaaaa
     4021 gcaccgacga aaatatgccc gacctgacca tagagcagct tcgtgccaac tgttccgtct
     4081 tcctcgttcc gcagttcgac gaaccggaag ccgtcgtttc ctacttcgac gaacgctacc
     4141 ggcagatttt cgaggcggag ctggcaggtt gggacatcga taaggataaa tggccgcaag
     4201 atatggggct gaaggcgttt tgggaatttt tcgacatcga aatccacgat atggtgttga
     4261 acatggaaga agcggaattg aacattaccc ccgtgttcga caacatgatg taaggctgtg
     4321 cgcgcctttc agacggcatt gaggccgtcc cgcatcctga acatcctgac tgtatcgctt
     4381 cacgccgcct cccttgccgt ctgcctgact tggttttacg gccggatgat gtggttcggt
     4441 ttggcggctt tggtggcaag ttacgcatac tcgctcagga taacgaatct gaaacaccgc
     4501 catgccataa ccgccattac catagaccgc gacggacagg cggaaatcgt atccggcaaa
     4561 gacaaaacgg caaaggcggc ggcactgtcg ggcagcagta tggttacgcc ttatgcctta
     4621 ttcttacaat gggacaccgg cggcaaaacc gtccgccaat gcatcatgcc cgacatgacg
     4681 gacaaagaat cctaccgcaa gctgaaagtt tgggtacttt ggcggcagcc gaaaaaaacc
     4741 gccgaaaccg atacatcaga ctgatttcag acggcatttg aaaacgaaac actcatgaaa
     4801 acactacaag actggctctc gcatttggaa accgcccaca gcggcggttt gatcgacatg
     4861 ggtttggagc gcacgagcga agtgaaaaaa cgcatgaagc tcgagccgca atgccccgtc
     4921 gtcgttgtcg cgggaaccaa cggcaaaggt tcggtctgtg cctacctgac acagatttac
     4981 aaacaggcgg gctacaaaat cggtacgctg accagcccgc atttattgcg ttacaacgaa
     5041 cgcatcgcca tcaacgccga acccgtttcg gacgatacga ttattgcctc tttcgagcgc
     5101 atcgaagccg cgcgcggcga aatatcactg acttattttg aattcaatgc cttggcggct
     5161 gtcgacatct tcatgcgcga acaggttgac gtgatgatat tggaagtcgg cttgggcggc
     5221 cgcttggacg cggtcaacgc gtttgacggc gattgcgcgg tggttaccag cgtggatttg
     5281 gaccatcagg catttttggg cgatacggtc gagcaggtcg gctttgaaaa agcaggcgtg
     5341 ttccgtagcg gcaaacccgc catctgcggt caaaaccccg cgcccaaatc gttgctttca
     5401 cacgccgaag ccataggcgc gaaactgctg atggtgcagc gcgatttcga gtttcacgcg
     5461 atggaaaaca tccaatggaa ctaccgcttc cggccgcaac attcagacga ccccgcgcgc
     5521 aaccgcaatg ccctgccctt ccccgcattg cgcggcacat accagctttc caatgccgcc
     5581 tgcgcgctga ccgtattgga atgcttgaac gacaaattgc cggtggacat cggtgccatc
     5641 aagcgcggtt tgttgctggt tgaaaatccc ggacgcttcc aagtcctgcc cggccggccg
     5701 ctgaccgttt tggatgtcgg acacaatccc cacgccgccc gcgccctgcg ccgcagcctg
     5761 attaatttgg cgtttgcgca aaaacgcacc gccgtgttca gcatgttgtc cgacaaagac
     5821 atagacggcg tgttggaaac cgttaaagac cagtttgacg agtggtatat cgcaccgttg
     5881 gatgtaccgc gcggcatgac gctcgagagc ctgcaaaaca aactgcacga acacagcatc
     5941 gaaaacatac aaacattcgc cgccgtccgc gaagcatacc gggcggcagc ctcaaaagcg
     6001 ggcgagaacg acagaatcgt cgttttcggc tcatttcata ccgttgccga tgtcatgtcg
     6061 gtactttaaa aggaaacttc tatgtccgaa aacaaacaaa acgaagtcct gagcggttac
     6121 gaacaactca aacggcgcaa ccgccgccgc ctcgtaacgg caagttgcct ggttgccgcc
     6181 tcctgcatcc tgctggcagc cgccctcagt tccggccctg ccgaacagac tgccggcgaa
     6241 acaagcggcg tagaaaacaa agcggcaggt gcggcacaaa cccctgcctt gaaatccgcc
     6301 gccgacaaac cgcaggactt ggcaggcgaa gacaagcctt ctgccgccga cagcgaaatc
     6361 agcgagcctg aaaacgtagg cgcgccgctg gtgctgatta acgagcgcct cgaagacagc
     6421 aacatcaaag gtttggaagc atccgagaaa ctgcaacagg cagaaaccgc caaaaccgca
     6481 ccgaagcagg caaaacaacg cgctgccgaa aaagtgccgg caactgccga cagtacggat
     6541 acggtagcgg ttgaaaaacc gaaacgcact gccgaaacaa aaccgcaaaa agcggaacgc
     6601 actgccaaag ccaagcccaa agccaaagaa accaaaaccg ccgaaaaagt tgccgacaaa
     6661 ccgaaaactg ccgccgaaaa aaccaaaccg gatacggcaa aatccgacag cgcggtaaaa
     6721 gaagcgaaaa aagccgacaa ggctgaaagc aaaaaaacag ccgaaaaaga ccgttcggac
     6781 ggcaaaaaac acgaaacggc acaaaaaacc gacaaagcgg acaagaccaa aaccgccgag
     6841 aaggaaaaat ccggtaaaaa agccgccatt caggcaggtt atgccgaaaa agaacgcgcc
     6901 ttaagcctcc agcgcaaaat gaaggcggcg ggtatcgatt cgaccatcac cgaaattatg
     6961 accgacaacg gcaaagttta ccgcgtcaaa tcaagcaact ataaaaacgc aagggatgcc
     7021 gaacgcgatt tgaacaaatt gcgcgtacac ggtatcgccg gtcaggtaac gaatgaatag
     7081 cctccccatt gccgacctcc tcgtctccgc cgtcatcgcc gcctgcatcg tgctatccgc
     7141 gatgcgcggc gtgattgcgg aggcaggctc aatggcggca tgggtggttt ccttcttttt
     7201 cgccaaactc tttgccgcct ccttcgccga cctcgccttt gcctcgttcc aaccccgcct
     7261 gtttgcattg gctctgtcgt tcatttccct gttcgtcatt gcctgtctga tccagaaaat
     7321 gctccgttcg ctgctgacca gcgcagtttc ggcggtcggt ttgggctttg ccaaccgcat
     7381 tttgggcggc gtattcggtg cattgaaagg cgttttgatt gttaccctgc tggtcatgct
     7441 tgcttcaaaa accgacctgc ccgataccga agaatggcgg caatcttaca cactgccgtt
     7501 tttcgtatcg ctttccgaag ccgtgttgaa ccatagcggc ggcacggcgg aaactccgga
     7561 agacgattga ttgccatacg gatatttcag acggcatcct cttttatgcc gtctgaaatg
     7621 ttttttcctt tcctcagcca tatcacag
//
LOCUS       DAWGRZ010000097         7619 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000097 DAWGRZ010000000
VERSION     DAWGRZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.097"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(100..1017)
                     /locus_tag="WHT44_001387"
     CDS             complement(100..1017)
                     /locus_tag="WHT44_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ2533563.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFADGA
                     HQVNRLTDALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            1190..1990
                     /locus_tag="WHT44_001388"
     CDS             1190..1990
                     /locus_tag="WHT44_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ2533564.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            2038..2814
                     /gene="mlaE"
                     /locus_tag="WHT44_001389"
     CDS             2038..2814
                     /gene="mlaE"
                     /locus_tag="WHT44_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ2533565.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            2865..3359
                     /gene="mlaD"
                     /locus_tag="WHT44_001390"
     CDS             2865..3359
                     /gene="mlaD"
                     /locus_tag="WHT44_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ2533566.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            3396..3986
                     /locus_tag="WHT44_001391"
     CDS             3396..3986
                     /locus_tag="WHT44_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ2533567.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVSQIRQNATQVLSILKN
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGGK"
     gene            4037..4315
                     /locus_tag="WHT44_001392"
     CDS             4037..4315
                     /locus_tag="WHT44_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ2533568.1"
                     /translation="MHTELKNGILHIGGDITVKTLTAAAFTRFRQQCRLKDTCAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            4312..5139
                     /locus_tag="WHT44_001393"
     CDS             4312..5139
                     /locus_tag="WHT44_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ2533569.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            5264..5626
                     /locus_tag="WHT44_001394"
     CDS             5264..5626
                     /locus_tag="WHT44_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ2533570.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            5623..6009
                     /locus_tag="WHT44_001395"
     CDS             5623..6009
                     /locus_tag="WHT44_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ2533571.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            6006..6494
                     /locus_tag="WHT44_001396"
     CDS             6006..6494
                     /locus_tag="WHT44_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ2533572.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(6575..6742)
                     /locus_tag="WHT44_001397"
     CDS             complement(6575..6742)
                     /locus_tag="WHT44_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ2533573.1"
                     /translation="MEDDVWPGHRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(6709..7089)
                     /locus_tag="WHT44_001398"
     CDS             complement(6709..7089)
                     /locus_tag="WHT44_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ2533574.1"
                     /translation="MGSKFFFLLLRFAGSGLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMEPECLFYSNNHKFDRS
                     KNALRATRKSVRLRWRTMSGRGTG"
     gene            7099..7225
                     /locus_tag="WHT44_001399"
                     /pseudo
     CDS             7099..7225
                     /locus_tag="WHT44_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7327..7542
                     /gene="rpmE"
                     /locus_tag="WHT44_001400"
     CDS             7327..7542
                     /gene="rpmE"
                     /locus_tag="WHT44_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEZ2533575.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT         1785 a         2206 c         1930 g         1698 t
ORIGIN      
        1 ttagagaatc gttctctttg agctaaggcg aggcaacgcc gtactggttt ttgttaatcc
       61 actatacacg cggatttcgg gtttgaaatc aaatgcggct taccggtttt cttttttggt
      121 ttccgcaggc gtgctgccga aatattgcct gaatgcctga gtaaagctgg aaacgtggcg
      181 gtagccgcat aaatatgcgg tttcgcctat gctttttccc ccgttttgca acagatagag
      241 cgcgtgctgc atttgtttgt ggtgcagcca ttcgcttgcc gtaatgccga aatggtcgcg
      301 catacggcgt tgcagcgtcc tttcactgat gttcagcgcg tctgtcagcc ggttgacttg
      361 gtgtgcgccg tcggcaaacg cggcatttag ggtgcggctg aagtcttcag acggcatagc
      421 gtctgcttcc gccgtttgcc ccgccgccgg cccgatgccg tctgaaaccg tgtcccacaa
      481 gtccgacagc agccgcaaca cgtccgcctc gcggcgcaat gtttcgccca aatgcccctt
      541 tgggacggct ttcaggcagg atgccgccaa gccgcgcagg ttcgggggca aatcccatat
      601 cctgaccggt tcgcggtaaa gcaggggtgc gaaacgcgcg tattccggac gcagcagcca
      661 ttgttccata cctttaatgg tcattttgac cgttttgccg cctcggtaaa gatagcggct
      721 gaacaggact tcttccccga cagcaattag gacaatcttg ccgccgtccg catcgatttg
      781 gaagcggcag cggttgatgc cgaagtccaa actgccttcc agcaagagca cgaacgacac
      841 ataaggttcg gcgaggcggc tgctgcaaaa atcacaccgt gccgttactg tgccgccgtg
      901 gatggaaatg ccgttggaca aagtatcgaa acggtagctg ccctccacat agctgccgcc
      961 gccgcttttg actagattga cgaaccggct gctgttcaaa tgttctgctt tttccatagt
     1021 tttctgataa aggttatcat ttgaaagata acatttttcg ccacagcaaa caatccgccg
     1081 ccgacaacgg cattttcaca acatttaccg tccgtatgcc gtctgaaaat tgcccgtctt
     1141 cccgataagc cttaaaatac acgccgtcaa acctaccgtc cgtccgtaca tgagcccatc
     1201 cccctttatc gaaatgaaag acgtcgcctt cgcgtatggc gaccgcccga ttctgaagaa
     1261 catcaatttc agcattccgc aaggcaattt tgctgccgta atgggcggtt cgggcagcgg
     1321 caaaaccacg ctgatgcgac tgattacagg acagattcgt ccgcagtccg ggcaggtttt
     1381 gattgaagga cgggatttgg cgggcttttc ggctgacgag ttgtatgaac accgccgccg
     1441 tatgggcgta ttgttccaac acggcgcgct gtttactgat ttgtcggtat tcgacaatat
     1501 cgcttttccg atgcgcgaac tgacgcgcct gccggaagcg gtgattcggg atttggtttt
     1561 gttgaaattg aacgcggtcg gtctgcgcgg tgtggaaaac ctgatgccgt ccgagttgtc
     1621 cggcgggatg tcgcgccgcg tcgcgcttgc ccgcacgatt gcgctcgacc ctgaaattat
     1681 gttgtacgac gagccgttta ccggcctcga tccgatttcc ttgggcgtga ttgcccactt
     1741 gatcagccgc gtcaacaagg ctttgcgttc gaccagtatt atggtaacgc acgacattga
     1801 aaaatctttg gaaatcgtcg atcaggtgat tttcttggcg cacggcgaaa ttatgttctc
     1861 cggctcgccg caggaaatgc gcgaactgga ttcgccttgg gtgcgccagt ttgtcggcgg
     1921 gctggcagac ggccccgtag cataccgtta tccggcgcaa acgtcgttgc agcaggacct
     1981 gttgggataa atgagttgat gccgtctgaa accgttgaac tttacacata gaaatttatg
     2041 aactttatcc gttccgtcgg ggcgaaaacc ctcggcctta ttcaatccct cggcagtatc
     2101 acgctgtttc tgctgaacat tctggcgaag tccggtacgg ctttcgtccg tccgcgcctg
     2161 agcgtgcgcc aagtgtattt cgccggtgtg ctgtcggtgt tgattgttgc cgtttcgggg
     2221 ctgtttgtcg gcatggtctt gggtttacag ggctatacgc agttgtcgaa attcaaatcc
     2281 gccgatattt tgggctatat ggtcgcggct tcgctattgc gcgagctggg tccggtattg
     2341 gcagcgattt tgtttgccag cagcgcgggc ggtgcgatga ccagcgaaat cggtctgatg
     2401 aaaacgaccg aacagctcga agcgatgaat gtgatggcgg taaaccctgt cgcccgcgtg
     2461 gttgcgccgc gcttttgggc gggcgtgttt tccatgccgc ttttggcttc gattttcaac
     2521 gtggcgggca ttttcggtgc gtatttggtt ggtgtaacct ggctgggctt ggacagcggt
     2581 attttctggt cgcaaatgca gaacaacatc acgatacatt acgatgtaat caacggtctg
     2641 atcaaatccg ccgcgttcgg cgtggcggta acgctgattg ccgtgcatca gggcttccat
     2701 tgcgtcccga cttcggaagg cattttgcgc gccagcacgc gcacggtggt ttcgtccgcc
     2761 ctgacgattt tggcggtcga ttttatattg accgcgtgga tgtttacaga ttgacaggcc
     2821 gtctgaagac gaaacgcgga acatcggata ttcaaggaac tttaatgaaa aagaacatat
     2881 tggaattttg ggtcggactg ttcgtcctga ttggcgcggc ggcggttgcc tttctcgctt
     2941 tccgcgtggc cggcggtgcg gcgttcggcg gttcggacaa aacttacgcc gtttatgccg
     3001 atttcggcga catcggcggt ttgaaggtca atgcccccgt caaatccgca ggcgtattgg
     3061 tcgggcgcgt cggcgctatc ggacttgacc cgaaatccta tcaggcgagg gtgcgcctcg
     3121 atttggacgg caagtatcag ttcagcagcg acgtttccgc gcaaatcctg acttcgggac
     3181 ttttgggcga gcagtacatc gggctgcagc agggcggcga cacggaaaac cttgctgccg
     3241 gcgacaccat ctccgtaacc agttctgcaa tggttctgga aaaccttatc ggcaaattca
     3301 tgacgagttt tgccgagaaa aatgccgacg gcggcaatgc ggaaaaagcc gccgaataaa
     3361 cgtatgccat catattcaaa ctaggacact taatcatgaa aaaatcctcc ctcatcagcg
     3421 cattgggcat cggtattttg agcatcggca tggcatttgc cgcccctgcc gacgcggtaa
     3481 gccaaatccg tcaaaacgcc actcaagtat tgagcatctt aaaaaacggc gatgccaaca
     3541 ccgctcgcca aaaagccgaa gcctatgcga ttccctattt cgatttccaa cgtatgaccg
     3601 cattggcggt cggcaaccct tggcgcaccg cgtccgacgc gcaaaaacaa gcgttggcca
     3661 aagaatttca aaccctgctg atccgcacct attccggcac gatgctgaaa ttaaaaaacg
     3721 ccaacgtcaa cgtcaaagac aatcccatcg tcaataaagg cggcaaagaa atcatcgtcc
     3781 gcgccgaagt cggcgtaccc gggcaaaaac ccgtcaacat ggacttcacc acctaccaaa
     3841 gcggcggtaa ataccgtacc tacaacgtcg ccatcgaagg cgcgagcctg gttaccgtgt
     3901 accgcaacca attcggcgaa attatcaaag cgaaaggcgt ggacggactg attgccgagt
     3961 tgaaagccaa aaacggcggc aaataaccga caaaccatac cgtccgaggg cggtatgggt
     4021 tttccgagca aacgctatgc atacagaact caaaaacgga atattgcaca tcggcggcga
     4081 catcaccgtc aaaaccctga ccgccgccgc ttttacccgc ttcagacagc aatgtcgtct
     4141 gaaagacacc tgcgcggttg atttgagcgg cgtcggacgc gccgattccg cctgcgtgtc
     4201 gctgctgctc gaagtgctgc gcggttgcaa aggcagcgtc aggctgaccg gcattcccga
     4261 atccgtgcgc gcgctgtccg aactgtacga aatcaaagac tggctgaaat catgaaaaaa
     4321 accgcctatg ccttcctcct gctgatcggg ttcgcttccg cccctgcatt tgccgaaacc
     4381 cgccccgccg acccttatga aggctacaac cgcgccgttt tcaaattcaa cgaccaagcc
     4441 gaccgctaca ttttcgcccc tgccgcgcgc ggctaccgca aagttgcgcc gaaacccgtc
     4501 cgcgccggcg tgtccaattt ttttaacaac ctgtgcgacg tggtcagctt cggcagcaat
     4561 atcttgcgct tggacatcaa acgcgcaagc gaagaccttg tccgcgtcgg catcaacacc
     4621 actttcggtt tgggcgggct tatcgacatc gccggcgcgg gcggcattcc cgacaataaa
     4681 aacaccttgg gcgacacgtt tgcctcgtgg ggatggaaaa acagcaatta tttcgtgttg
     4741 cccgtcttag ggccgtccac cgtccgcgac gcgctcggca cgggtattac ctccgtttat
     4801 tcgcccaaga atatcgtctt ccgcacccct gtcggacgct ggggcacgac tgccgtatcc
     4861 gccgtcagta cgcgcgaagg cctgctcgat ttgaccgaca gtctggacga agccgccatc
     4921 gacaaataca gctacacgcg cgacctctat atgaaagtcc gtgcgcggca gaccggtgca
     4981 acacctgccg aaggtacgga agataacatc gacatcgacg aattggtcga aagtgccgaa
     5041 accggcgcgg cggaaactgc cgttcaagaa gattccgtat ccgaaacaca ggcagaagca
     5101 gcaggggaag ccgaaacgca acctggaaca caaccttaaa gccatgccgt ctgaaaatcc
     5161 gtgttcggac ggcattccct tctgtcggaa cggacagtcc gaaaccacag attgggctat
     5221 aatctctttt ttgcgcatac cgcgcgacag caaaggaaaa accatgtacg aagtcaaccg
     5281 cagcgtattc gtcctcatcc ctttagaacc tttttggaac tggctgcaaa ccctgccggg
     5341 caaccacctc gacggactga ctcttgaaga catccaagcc gacgccaatt cctaccttgt
     5401 ccgcccgtgc gaaaccgccg acgaagtatg ggacgaaatc gaagcccgtt ttgaagacat
     5461 tttcgccgcc gaacttgccg actggtgcga agacgaacgc gagtggcctg cgctcgatgc
     5521 cgacattttc aacgaatggt tcgacatcca gctttctacc gtcatcaccg acctcgaaca
     5581 cgaaccgctt gcccgcgaag ccttccaacc catcaacctg aactgatgaa gctgaccgtc
     5641 cgcaactacc atctcgacgg ctacggccat gtcaacaatg cgcgctacct cgaatttttc
     5701 gaagaagcgc gctgggcgtt ttttgaagaa cgcggactgc tgcacgagtt ggcaggcctc
     5761 atactgattg tcgcccgcat cgacatccga tacagccgcc ccgccgtcga aggcgacgta
     5821 ttgcagtttt catgccgcct gaaaaccccc ggcacgcgcc gtattgtgct gacccagacg
     5881 attacactgc caaacggcaa aaccgccgca gaagccgaca tcaccctgat gcccgtccac
     5941 gccgccacac aacgcaccgt cagcctgccc gccacccttg cccgcgcatt ggaagccttg
     6001 tccgaatgaa aaaaatactc accgccgccg tcgtcgcact gatcggcatc ctccttgcca
     6061 tcgtcctcat ccccgacagc aaaaccgcgc ccgccttctc cctgcccgac ctgcacggaa
     6121 aaaccgtttc caacgccgac ctgcaaggca aagtaaccct gattaatttt tggtttccct
     6181 cctgtccggg ttgtgtgagc gaaatgccca aaatcattaa aacggcaaat gactataaaa
     6241 acaaaaactt ccaagtactt gccgtcgccc agcccatcga tccgatagaa agcgtccgcc
     6301 aatatgtcaa agactacggt ttgccgttta ccgtcatgta tgatgcggac aaagctgtcg
     6361 gacaggcgtt cggcacacag gtttatccga cttccgtcct tatcggcaaa aaaggcgaaa
     6421 tcctcaaaac ctacgtcggc gaacccgatt tcggcaaact ctaccaagaa atcgataccg
     6481 cgctggcgca atagcgatgc cgtctgaagc gcaaaaaaca gaaacgatgt tcctgttttt
     6541 tcagatcatc cgccccgaca cgttcagacg gcattcaacc ttccggcaga ttctttttca
     6601 ccactgccgg attgcctgcc gccaaagagt agggcggaat gtcttttgta accaccgcgc
     6661 ctgcgcccac gaccgaaccg cgtccgacgg ttacgcccgc cattacaatc accctgtgcc
     6721 ccggccagac atcgtcctcc aacgtaatcg gacggatttc cgtgtagccc tcaaagcgtt
     6781 ttttgaacgg tcaaacttgt ggttatttga ataaaacaga cattccggct ccatcatcac
     6841 atttttgccg accaccagcc cacggcagat ttcacagttt gccccgatgc ccgagccgtc
     6901 gcccaaaacc gtatccggaa acacatacgc cccgcgttcg atattgaccc cgcgtccgat
     6961 atgcggagaa acccgccgcg ccaaaaaacc gcgcacccgt ctgccgacga tgccgatgcc
     7021 gcgcatatgt gacggcggca accccgaacc ggcaaaacgc agcagcagga aaaagaattt
     7081 tgatcccatc aaacctccct caaaagaact ttttatagtg gattaacaaa aattaggaca
     7141 aggcaacgaa gccgcagaca gtacagatag tacggcaagg cgaggcaacg ctgtactggt
     7201 ttttgttaat ccactataca ttttatcgaa tatactcttg acgtaacaac agaaaatagc
     7261 gagaatgtcg gacttgtctg tagcataggt acatccccta tgcccgcttt tttgaaagga
     7321 aatcaaatga aacaaggtat tcacccgaac taccacgaag tcaacgttac ctgctcttgc
     7381 ggcaacaaat tcgcaaccaa atccgcgatg gaaaaagaaa acttcaatat cgaggtttgc
     7441 tccctgtgcc acccgttcta taccggcacc caaaaaatcg ttgataccac cggccgcgtg
     7501 gacaaattca acaacaaatt cggcaacctg ttcaaacgct gattgccgct ttgcaagaat
     7561 taagccctgc catcatttcg atggcagggc tttttaatat ttggcacact gacttctta
//
LOCUS       DAWGRZ010000098         7585 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000098 DAWGRZ010000000
VERSION     DAWGRZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.098"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(53..361)
                     /locus_tag="WHT44_001401"
     CDS             complement(53..361)
                     /locus_tag="WHT44_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533576.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDALKDLLADLDKELC
                     "
     gene            complement(345..647)
                     /locus_tag="WHT44_001402"
     CDS             complement(345..647)
                     /locus_tag="WHT44_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ2533577.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(721..3084)
                     /locus_tag="WHT44_001403"
     CDS             complement(721..3084)
                     /locus_tag="WHT44_001403"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ2533578.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGEVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNARATTPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLDRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     CVFEIARVFSKGSDGQFVQNERIGGLWYGAVMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRVVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3109..3759)
                     /locus_tag="WHT44_001404"
     CDS             complement(3109..3759)
                     /locus_tag="WHT44_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ2533579.1"
                     /translation="MITISNEDNMILMSRYPDKYFDLAIVDPPYGILNKTKRGGDYKF
                     NMNEYSQWDIKPDQTYFNELFRVSKNQIIWGGNYFGELWLRSEYNKGFIIWDKNQPET
                     LNNFSMAEMAWSSFDRPSKIFRFSVRKNRNKTHPTQKPVELYQWLLKMYAKQGDKILD
                     THLGSGTLAIACCIAQFDLTACEINSDYYQQSIEKIKNNLPEARISFGHPGYCIIE"
     gene            complement(3756..5225)
                     /locus_tag="WHT44_001405"
     CDS             complement(3756..5225)
                     /locus_tag="WHT44_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ2533580.1"
                     /translation="MTTARWVIDKHLQNFHILCKFAGILKTNSFISVEDKAKLSEKLE
                     KLDLYHRRNTGKSLDATTHKIKELSFYMFGYRDVCGQVTQKFLFSPLGNLFLKHLDNN
                     EYIQKIFLTMLWAIPFPHPYIKTDESIQLYPMRLIFKLLSDERLDCKLFSYEYIYLIS
                     FVKSADQNSYEKLVQDILVLRTCAEVKIKHQLTAENSRSHAYVNAAHEWESYFSKTLT
                     DAGVLQKTDGKIICRLKHGKTETYRKVTSSEFSIPKQLQEFVKKLQSAYSFSEMPLNL
                     NDSDRLKIDVIKEIYSFYPKELLEEIGELKDEAAYELLHLPRLIEQYADNNNGTEAYL
                     FEDVLEMGFNMFYNVEAKKIGGPGNTDLECLYITQKRKFAVEAKSTKNKLSGINSGRL
                     EDHKNKIKAIYTIVVTPRYVPAVLSDIRNCPIVIIRANTFAEFLYNCLINRSSIPEID
                     YRYFDEIIIKNLGKDISSEISNLTMQQFASNTTMEAYST"
     gene            complement(5228..6286)
                     /locus_tag="WHT44_001406"
     CDS             complement(5228..6286)
                     /locus_tag="WHT44_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ2533581.1"
                     /translation="MMIGASLFSSAGIAETYLHNAGIKIIAANELVPERANLYKALYP
                     ESKMIIGDILHEEVFQNLIQSVPNRLDFLIASPPCQGMSVAGKNRNIQEMANDKRNHL
                     IFRVIEFILLKRPTFVLIENVPFFLKIKLPYKGTLQTVEVILQDLFGCEYYIQTHIFD
                     SADYGVAQHRKRAIIRMNKHSTIWGMPEKVTKTISVRDAISFLPSIESGQKSNVKWHF
                     ARTHAPEHIIWLKNTPTGRSAFDNIEHYPKKKNGEKIKSYNTTYRRMEWDAPAPTITI
                     RNDAISSQLNVHPGRSMPDGTYSDARVLTPLELMLLTSLPIDWNIPDDTSELLIRQCI
                     GESIPPLLIKKIVERIGK"
     gene            complement(6469..7461)
                     /gene="pheS"
                     /locus_tag="WHT44_001407"
     CDS             complement(6469..7461)
                     /gene="pheS"
                     /locus_tag="WHT44_001407"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ2533582.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKREALNEVKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFAK"
BASE COUNT         2068 a         1808 c         1495 g         2214 t
ORIGIN      
        1 attttttagc gtgtaaaaca aaaccggctg catacctgca accggcctca aatcagcaca
       61 attccttatc caaatccgcc aacaggtctt tcagagcgtc tccgatttcc tgaagcgtaa
      121 ccggacggtt gccgtccgaa cccgattccg caccttctgc atacggtgca aacgtgctct
      181 gcaatttcaa cagtttcacc acatccaaac gggtgtagcg ttcgccgccg taaccgacaa
      241 ccacaccgtg atcatgctgc cattgcgcaa aaccataggg gctgatttgc aacagtccgc
      301 acaactcgtc cagcgtgaaa tagcgttttg cgggaacctt caaatcaacg ttgtttgtca
      361 tagtagtgtt ccaccatgct tttgagtttc tggctggcat ggaaagttac cacgcggcgg
      421 gcggtaatcg gcacttcctc gccggtttta ggattacgac ccgggcgttg cggcttatcg
      481 cgcaactgga aatttccgaa accggaaatt ttgatttctt cgccgcttgc caaagtgctg
      541 cggatttctt caaaaaagag ttcgacgatt tctttggcat cgtttttggt gacgttgctg
      601 actttgtcta ccaaaatatc ggccagttct gctttagtga gagtcatatg attaccttct
      661 gttgtaacag ttgtggatta ttaccaattt ttatttaaaa ttcaagcgaa tatttatttt
      721 ttaactgcga agccgcgccc ctgccgccgt tgccgcgcca atcagttttc cgataagcgg
      781 ctcgactgcc tcatccgtca gcgtgttttc catatcctgc aaaatcactt tgaccgccac
      841 gctcttcatc ccttcgggca gtcccgtgcc gcgatacacg tcaaacacgc tgatttcctg
      901 taccaacttg tttgccgcgc ctttcaagac aagcagcaaa tcatcatggc tcatagcttc
      961 cggcatcaca aacgccaaat cgcggcgcac cggctggaat ttcgatacga cccgatagcg
     1021 cgttttcccg cattccaaca cggccgccat atcgatttca aataccagcg gcgcttgcgg
     1081 caggtcgtat ttttgcagcc atttcggatg cagttcgccg acaaagccga tgactttgcc
     1141 gtctgaaacg atattggcgg cacgtccggg atgcagggcg ggatgtccgg ttttaacgaa
     1201 ctcgactgct ttgtttttca acagattttc cacgtccgcc ttgatgtcgt aaaaatccgc
     1261 attgcgcgtt ttcccgcccc attgttccgg catgaccgcg ccgtaccaca atccgccgat
     1321 gcgttcgttt tggacaaact ggccgtctga acctttgctg aacacgcggg cgatttcaaa
     1381 cacgcacacg cggttttgtt tgcggttcag attgttttgc agaatttcca ccaagccgcc
     1441 gatgagcgtg gaacgcatca cggcatactg cgccgccagc gggttttgca ggcggatggg
     1501 gtcggcgttg gcggcaaaat cttgttccca ctgctcgtca acgaaggcat agctgaccac
     1561 ttcgcggtaa ccgcgagccg ccatttcgtt gtaaacggca aaacgcgggc ggcgtgtttc
     1621 gggcagttcc agcattttca gacggcctga cgtgtaatcg tcggggatgt tttcatagcc
     1681 gtaaacgcgt ccgatttctt caatcaaatc agcctcaatt tcgatgtcaa aacggaagct
     1741 cggcgcggta acgcggaagc cttccgccgt tttctcgggc tgcaggccca agtgttgcaa
     1801 aatggtttcc acctgttcgg caggaatgtc cacgcccaac acggttttca gacggtccaa
     1861 acgcaatcca acctgcttcg cttcaggcaa ttcgccttgc gcttccacca tctcgcctgc
     1921 cgcaccaccg caaatctgca acaccaattc ggtagcacgt tcaatggcat ccgcctgcaa
     1981 acggtaatcc acgccgcgct cgaagcggaa cgacgaatcc gaaccgaaac cgtattggcg
     2041 cgatttgccg gcgatgattt cgggcgcaaa ccaagccgct tccagcacga tattttgcgt
     2101 gccgtctgaa accgcgctcg ccgcgccgcc cattaagccc gccaaactca acacgccttt
     2161 ttcgtccgcc acgaccagcg tgttttcaga cagggaaacg gttttctcgt tcaggcattc
     2221 cagcgtttcc ccttcgcgcg cgcggcggat gtgcaggctg ccggaaagtt tgtcggcatc
     2281 aaaaacgtgc atcggctgac cgatttccag catcacataa ttgccgatgt ccaccagcgc
     2341 ggaaatactg cggatgccgc tgcgctccaa acgttgtttc atccaatccg gcgtagtagc
     2401 gcgcgcgttc acgttttcaa tcacacggct gataaaacgg ccgcaatcgg caggcgcgtt
     2461 aatctgcacg ggctgttttc gactgcccgt gatcggcgcg gtatggattt cgggctgcct
     2521 gaacgcgcac cccgtcaatg cggacacttc gcgcgcaatg cctttgatgc tcaagcagtc
     2581 ggcgcggtta ggcgtaattt tcaacgtaaa cagcgtatcg tccaaatcca agtattcgcg
     2641 gatattggta ccgacgggcg catcttcagg cagaatgtgc aggccgttca caccgtcgtc
     2701 gggcagaccg agttcgtcgg tggaacacaa catcccgtcc gacacctcgc cgcgcatttt
     2761 ggtcggcttg attttgaaat tacccggcaa aacggcaccc ggcagcgaac acggcacttt
     2821 gatgcccgct ttcacattcg gcgcaccgca cacaatctgc accaacccgc ccgtacccgc
     2881 atcaactcgg gtaacgttca aacggtctgc atccggatgt ttttcaacgg atttcacttc
     2941 ggcaatcacc acgcccgcaa acgcaggcgc ggcagtttca gcctcttcca cttccaagcc
     3001 ggacatcgtt aacagatgtt ccagcttatc ggaggaaagt tcggtatcgg cttgggtttt
     3061 cagccatgag taggagaatt gcatggttaa tttctctata tttttaagtt attcaataat
     3121 acaataacct ggatgcccaa aactgattct agcttcaggt aaattatttt ttattttctc
     3181 aatcgattgt tggtaataat cggaattgat ttcacaagct gtcaaatcaa actgtgcaat
     3241 gcagcatgca atagcaagag ttccacttcc taaatgtgta tctaaaatct tatcaccctg
     3301 ctttgcatac atttttaaca accactgata taattcgact ggtttttgtg ttgggtgagt
     3361 tttattacga tttttccgca cactaaaccg gaaaatttta gatggcctat cgaatgacga
     3421 ccaagccatt tccgccatag aaaaattatt taatgtctct ggttgattct tatcccaaat
     3481 aataaatcct ttattatatt cacttctcaa ccataactcg ccaaaataat tcccacccca
     3541 aataatttga ttttttgaca cgcgaaataa ttcattaaag taagtttggt ctggcttaat
     3601 atcccattgt gagtattcat tcatattgaa tttataatca ccaccacgtt tagttttatt
     3661 caaaatccca taaggaggat ctacaattgc caaatcaaaa tacttgtcag gataccgaga
     3721 cattaagatc atgttatctt catttgaaat agttatcatg tactatacgc ttccattgtg
     3781 gtgttacttg caaactgttg catagtcaaa ttggaaattt ctgaactaat atcttttcca
     3841 agatttttaa taataatttc atcaaaatac cgataatcaa tctctggaat actggagcga
     3901 ttaatcaaac aattatataa aaattcagca aatgtattgg cacgaataat tacaattggg
     3961 caattacgaa tatcggataa tacggcaggg acataacgtg gtgtgacaac aattgtgtaa
     4021 atggccttaa ttttattttt atgatcttcc aatcttcctg aattaatacc tgataactta
     4081 tttttagttg attttgcctc cactgcaaat tttctctttt gcgtaatata taagcactct
     4141 aaatccgtat tacctggtcc accaattttt ttagcttcta cgttataaaa catattgaac
     4201 cccatttcta gaacatcttc aaatagatat gcctctgttc cattattatt atctgcatat
     4261 tgttcaatca acctaggtaa gtgcaataat tcatatgctg cttcatcctt aagctcacca
     4321 atttcctcca ataactcttt tggatagaag ctataaattt ccttaatgac atcaattttc
     4381 aaacgatcac tatcgttcag atttaatggc atttctgaaa acgaataagc actttgcaat
     4441 tttttcacaa attcctgaag ttgcttagga atcgaaaact cacttgatgt tactttacga
     4501 tatgtttcgg tcttaccatg ctttagacgg caaataattt taccatctgt tttttgcaaa
     4561 acacctgcat cagtcaatgt ttttgaaaaa taagattccc actcatgtgc tgcatttaca
     4621 taagcatgac tacgactatt ttccgcagtt aattgatgtt taatttttac ttcagcacat
     4681 gttcgtaaca ccaaaatgtc ttgtactaat ttttcatagc tattctgatc agcagatttc
     4741 acaaatgaaa ttaaatagat atattcataa gaaaatagtt tacaatccaa tctttcatca
     4801 gataacaact taaatattag tctcatggga tataattgaa tactttcatc agtcttaatg
     4861 tacggatgag gaaatggtat cgcccacaac atagtaagaa aaattttttg aatatattca
     4921 ttattatcca agtgtttcaa aaataaatta cccaatggac tgaacaggaa tttctgtgta
     4981 acttgcccac acacatcacg ataaccaaac atatagaatg ataattcttt tattttatga
     5041 gtagtagcat ccaatgattt acctgtattt cgtctatggt ataaatctag tttttccaat
     5101 ttttcagata acttagcttt atcctctaca gatataaaag aatttgtttt caaaatacct
     5161 gcaaatttac ataaaatatg aaaattctgt aaatgtttat ctattaccca gcgcgcagtt
     5221 gtcatatcta ttttcctatt ctctctacaa tttttttaat taacaatgga ggaatagatt
     5281 caccaataca ttgccgaatt aataattctg atgtatcgtc gggaatattc caatcaatag
     5341 gtaatgatgt taaaagcatt aactctaatg gtgttaaaac tcttgcatct gaatatgttc
     5401 catcaggcat agaccgtcca ggatgaacat ttaattgtga actgatagcg tcattacgaa
     5461 tagtaatagt tggggcagga gcatcccact ccatacggcg ataagttgta ttataacttt
     5521 taattttttc accatttttt ttctttggat aatgttctat attatcaaaa gcagatcgtc
     5581 ctgttggcgt attttttagc catataatgt gctccggagc atgtgtacgt gcaaaatgcc
     5641 atttcacatt agacttttgt ccagactcaa tactaggcaa aaaactaata gcatcacgaa
     5701 cagaaatggt ttttgtaact ttttccggca ttccccaaat agttgaatgt ttattcatac
     5761 gaataatagc tcgtttacga tgttgtgcaa caccataatc ggcagaatca aaaatatgag
     5821 tttgaatata atattcgcaa ccaaataaat cttgcaaaat tacttctact gtttgtaatg
     5881 tccccttata aggtaactta atttttaaaa aaaatggaac attttcaatc aaaacaaaag
     5941 ttgggcgttt taataaaata aattcgatta ctctgaatat taaatgatta cgtttatcat
     6001 tagccatctc ttgaatgtta cgatttttcc ctgcaacact catgccttga caaggaggag
     6061 aagcaattaa aaaatctaat cgattcggca cgctctgtat taaattttga aacacttcct
     6121 catgaagtat atcacctata atcattttac tttcgggata tagagcttta tataaattag
     6181 cacgttctgg caccaattca tttgcagcta taatcttaat acccgcatta tgcaagtagg
     6241 tttctgcaat ccctgcactc gaaaacaaag aagcccctat catcatagaa ccaaacccaa
     6301 attaactata taatctctac aaagcaataa attatgcctc tttaaacaaa attaggaatg
     6361 taatttcaca acaaaatatc aaatgtttta tatcttacat ttgaattttc ctaagcagcc
     6421 tgaaaaccaa gagtaggctg cttttccata ttcaggcagt ctgcacgatc atttcgcaaa
     6481 ctgcttcaaa aagttcaaat cattatcgaa gaacaggcgc aagtcgttca cgttgtaacg
     6541 cagcatagcg aagcggtcga gaccaatacc aaaggcgaaa ccggtatatt tttcagggtc
     6601 gatattgacg tttttcaaca cgttaggatg taccataccg caaccgccta cttccagcca
     6661 tttgccgttt tcgcccataa tgtcgatttc ggcagacggt tcggtgaacg ggaagaaaga
     6721 cgggcggaaa cgtacttgca aatcatcgcg ttcaaagaag cgacggataa aatccgtgaa
     6781 cactgctttt aagtcggcaa aagttacgcc ctcttctacc cacaaacctt ccgcctgatg
     6841 gaacataggc gagtgcgtgg catcgctgtc cacacggtaa acgcggccgg gggcgataat
     6901 gcggatgggc ggctcttttt tatcgagcat atagcggatt tgaatcgggg aagtgtgcgt
     6961 acgcaaaaca tcgccgtttt caacgtaaaa cgtatcctgc atcgcacggg caggatggtt
     7021 tgcagggatg ttcagggctt ggaaattgtg aaaatcgtct tcgatttcag gcccgtccgc
     7081 cacttcgaaa cccattccgt gaaagagttc gaccacacgt tgcaaggtca gggttacggg
     7141 atgcaggctg ccgccttcct gagcgcgtcc gggcagggta atatcgaggg cttcggcggc
     7201 aagtcgggct tgcagcttga cttcgttgag ggcttcgcgt ttggcattaa aagccgtctg
     7261 aaaccggttt ttgcattcat tgatatgcgc acctatggtt ttgcgctctt caggcgacat
     7321 ttgccccaaa gttttcagaa gtccggtcaa ctcgccggtt ttaccaagat aacgggcttt
     7381 gatttgttct agagcgttga agtcttgcgc agcttctact gcggcaatgc cttctgcaac
     7441 gatgcggttt acattttcca taatatcaag tctgtcaatt aatgatatac cacctgaaac
     7501 aacgaggccg cctgtatagc ctgacatatc agactcttca gacgacctta ttttcttcac
     7561 ggtcaatcca ttggataaat tttct
//
LOCUS       DAWGRZ010000099         7527 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000099 DAWGRZ010000000
VERSION     DAWGRZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.099"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            15..420
                     /locus_tag="WHT44_001408"
                     /pseudo
     CDS             15..420
                     /locus_tag="WHT44_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215060.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(455..1330)
                     /gene="rsmI"
                     /locus_tag="WHT44_001409"
     CDS             complement(455..1330)
                     /gene="rsmI"
                     /locus_tag="WHT44_001409"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ2533583.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREAGFKVVPVVGASAVMAALSVAGVEGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            1376..1723
                     /locus_tag="WHT44_001410"
     CDS             1376..1723
                     /locus_tag="WHT44_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ2533584.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGVAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            1728..2321
                     /locus_tag="WHT44_001411"
     CDS             1728..2321
                     /locus_tag="WHT44_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ2533585.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            2385..2993
                     /locus_tag="WHT44_001412"
     CDS             2385..2993
                     /locus_tag="WHT44_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ2533586.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3001..3633
                     /locus_tag="WHT44_001413"
     CDS             3001..3633
                     /locus_tag="WHT44_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533587.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     AAIGALAIGIVSTFNPNGDKTLQAEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            3673..4452
                     /gene="map"
                     /locus_tag="WHT44_001414"
     CDS             3673..4452
                     /gene="map"
                     /locus_tag="WHT44_001414"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ2533588.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4607..4906
                     /locus_tag="WHT44_001415"
     CDS             4607..4906
                     /locus_tag="WHT44_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ2533589.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5144..5518
                     /locus_tag="WHT44_001416"
     CDS             5144..5518
                     /locus_tag="WHT44_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ2533590.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTDNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5706..7172)
                     /locus_tag="WHT44_001417"
     CDS             complement(5706..7172)
                     /locus_tag="WHT44_001417"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ2533591.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEDRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1927 a         2264 c         1851 g         1485 t
ORIGIN      
        1 aagaggcgga atgggtgggc agcctgttcg gcctgcacaa atctttcggt ttccttacgc
       61 tgacggtgat tacattgcgc atcgtgtggg cggttgccaa ccgcgccaag cgtccgcaaa
      121 gcgactgcaa ggctgcggcg gcaggccacg gcattctgta tctgctcatg cttgctgttc
      181 ccgttatcgg catgatccgc caatacggca gcggccgcgg cccgttgaaa gtgttcggcg
      241 ttgaagtgat gcaagggttc gccggaaaaa atcgagtgga tggcaaactt gggcaacacg
      301 ttccacggca atttgggctt gctgctgttt gccgccgtcg ccggacacgt cgccatggtc
      361 gtcgcccacc gtgttcaggg tagagatgtt ctgtgccgca tgacgggtcg tgtccgctga
      421 ttccgttcac actatggtgc cggctcgtcc ggcactattt gtttttccaa gacagagcca
      481 gatcgtacaa agctttcttt ccctcgcccg tgattttggc agcaagctcc gccgcctgtt
      541 tggtcggcag ctcggctgtg aggattttca tgatgttttg cgcggactcg gacaagcctt
      601 cgtgtttttc atcctgcgcc ggataaagca ccaacaccat ctcgccgcgc gattggttgc
      661 cgtcggcaga caatgccgtc tgaatttccc caaccgtgcc gcttaagaac gtttcaaacg
      721 ttttcgtaat ttcgcgcgcc agcattaatc ggcgttcggg gaacagttcc gccatatcgg
      781 caagcgtcgc accgatgcgg tgcggcgttt caaacatgac gataggaaac gccgcccgca
      841 cccatttggc aaacagtttc ctgcgttctc ccgatttcgg cggtacaaaa ccgttgaaat
      901 aaaaatcgga tccttccaca ccggccacgc tcaaagccgc catcaccgcg cttgcgccca
      961 cgacgggaac gactttaaac ccggcctcac gcacgcggcg ggcgagtttc gcgcccgggt
     1021 cgcacacggc cggcgtaccc gcatcggaaa cctgtgccac aaccatgccg tctgaaagat
     1081 agccgacaat cttgtccgcc atctgccgtt cgttgtgttc gcgcacactg acgagtttgc
     1141 cctgaatgcc gtacgcgctc aaaagctgtg cggtaacgcg cgtgtcttcg gcacagatga
     1201 tgtccgcctt ttgcaatacc gccaaagcgc gcagggtaat gtccgccaaa ttgccgatgg
     1261 gcgtggcaac cacgtataat gtccctccga cgacgctgtc ggaggctttc tgcaaatgtt
     1321 tctgaaacat aagaatgccg tctgaaaaac aaacattata aaggttaaac cgattatgcg
     1381 cctaaaccac aaacagggcg aggcagggga agatgccgcg cttgccttcc tccaatccca
     1441 aggctgcacg ctgcttgccc gcaactggca ctgcgcctac ggcgaaatcg acctgattgt
     1501 caaaaacggc ggcatgattc tgtttgttga agtaaaatac cgcaaaaatc ggcaattcgg
     1561 cggtgtcgca tacagcattt ccccatccaa attattgaaa ctgcaacgaa gtgtagagta
     1621 ttatctgcaa cagaacaggt tgacaaacgt accgtgccgc ctcgatgcgg tacttatcga
     1681 aggcagccgc ccgcccgagt ggatacagaa tattacaggt tgacgatatg acgacattac
     1741 aagaacgcgt tgccgcccat tttgccgaaa gcatccgtgc caagcaggaa gccggaaaag
     1801 tattggtcga gccgaccgta caggctgccg agctgatgct gcaatgcctg atgaatgacg
     1861 gcaaaatcct ggcctgcggc aacggcggtt cggctgccga cgcgcaacac ttcgccgccg
     1921 aaatgaccgg ccgttttgaa aaagaacgca tggaactcgc cgctgtcgcg ctgacaacag
     1981 acacttccgc gctgacagcc atcggcaacg actacggttt cgaccacgta ttcagcaaac
     2041 aggtgcgcgc gctcggacgt gcaggcgatg tattggtcgg catttccacc tccggcaatt
     2101 ccgccaacgt catcgaagcc gtcaaagccg cacacgaacg cgatatgcac gtcatcgcct
     2161 tgaccggccg cgacggcggc aaaatcgccg ccatactcaa agacaccgac gttttgctca
     2221 acgttcccca tccgcgcacc gcccgtattc aagaaaacca catcctgctg atacacgcca
     2281 tgtgcgactg tatcgactcc gtactgctgg aaggaatgta acccttttca gacggcatgg
     2341 cgcaaagcaa tgccgtctga aacgcccaag aaaggaagca cccgatgaaa cccaaaccgc
     2401 acaccgtccg caccctgatt gccgccattt tcagccttgc ccttagcggc tgcgtcagcg
     2461 cagtaatcgg aagcgccgcc gtcggcgcga aatccgccgt cgaccgccga accaccggcg
     2521 cgcaaaccga cgacaacgtt atggcgttgc gtatcgaaac caccgcccgt tcctatctgc
     2581 gccaaaacaa ccaaaccaaa ggctacacgc cccaaatctc cgtcgtcggc tacaaccgcc
     2641 acctgctgct gctcggacaa gtcgccaccg aaggcgaaaa acagttcgtc ggtcagattg
     2701 cacgttccga acaggccgcc gaaggcgtgt acaactatat taccgtcgcc tccctgccgc
     2761 gcactgccgg cgacatcgcc ggcgacactt ggaacacatc caaagtccgc gccacgctgt
     2821 tgggcatcag ccccgccaca caggcgcgcg tcaaaatcgt tacctacggc aacgtaacct
     2881 acgttatggg catcctcacc cccgaagaac aggcgcagat tacccaaaaa gtcagcacca
     2941 ccgtcggcgt acaaaaagtc atcaccctct accaaaacta cgtccaacgc tgactcggca
     3001 atgccgtctg aaccgccttc agacggcatt gcccgacacc ccaaaagcac aatcaaaatg
     3061 gcaaaaaaac cgaacaaacc cttcaggctg acccccaaac tcctgatacg cgccgtattg
     3121 ctcatctgta tcgccgccat cggcgcattg gcaataggca tcgtcagcac attcaacccg
     3181 aacggcgaca aaacccttca agccgaaccg caacacaccg acagcccccg cgaaaccgaa
     3241 ttctggctgc caaacggcgt agtcggacaa gatgccgccc aacccgaaca ccaccacgcc
     3301 gcctcatccg aacccgcaca gccggacggc acagacgaaa gcggcagcgg actgccgtcc
     3361 cctgccgcac ccaagaaaaa ccgggtcaaa ccgcaacctg ccgacacagc tcaaaccgac
     3421 aggcagccgg acgacgccgg aacacaagct gaaaacacac tcaaagaaac ccccgtactg
     3481 cccacaaacg tcccccgtcc cgaaccccga aaagaaacac ccgaaaaaca ggcgcagccc
     3541 aaagaaacgc ccaaagaaaa ccataccaaa ccggacaccc cgaaaaacac gccgcccaaa
     3601 ccccataaag aaattctcga caacctcttc tgacccggca cggcaggcac acccgcaatc
     3661 caaggaagca ttatgaacgg catcatcatc aaaacccccg aagaaatcga aaaaatgcgc
     3721 gagctgggca aactcgtcgc cgaagccctc gactacatcg gacaattcgt caaacccggc
     3781 gtaaccaccg acgaaatcga caaactcgtt tacgactacc acgtcaacgt ccaaggcggc
     3841 tatcccgccc ccctgcacta cggcaacccg ccctacccca aatcctgctg cacctccgtc
     3901 aaccacgtca tctgccacgg cattcccgac gacaagccgc tcaaagaagg cgacattatc
     3961 aacatcgacc tcaccatcaa aaaagacggc ttccacggcg actccagccg tatgtttacc
     4021 gtcggcaaag tctcccccat cgcccaacgc ctgatcgacg taacccacgc ctccatgatg
     4081 gcgggcatag aagccgtcaa acccggcgcg acactgggcg acgtaggtta cgcctgccaa
     4141 caggttgccg aaaacgccgg ctattccgtc gtacaggaat tctgcggaca cggcatcggg
     4201 cgcggtttcc acgaagcccc gcaagtgttg cactacggaa aaaaaggaca gggccccgtt
     4261 ctaaaaccgg gtatgatttt taccgtcgaa ccgatgatca accaaggcaa acgccacctg
     4321 cgtatcctca acgacggctg gacggtggtt accaaagacc gctccctctc cgcccaatgg
     4381 gaacacgaag tcttggtgac cgaaaccggc tacgaaatcc tcaccgtcag ccccgcctcc
     4441 ggcaaaccct gaaaccggac gtatccgccc cataaaaaca aacaatgccg tctgaaagaa
     4501 acggcagata tgatatataa tataaaaaca ggcttgaccc ggcacattac gaaaacaaag
     4561 caaatcggaa tttgccccgc aaccagacaa acttaaagga agttttatga aaatatttga
     4621 aaatatagaa gatgttaaag ccatccgtaa aaagaccggg ctgaaccaga tagacttctg
     4681 gggcaaggtc ggcgttaccc agtccggagg atcgcgctac gaaaccggcc gcaaaatgcc
     4741 caaacccgta cgcgaactgc tccgcctcgt ccatatcgaa tgcatcgatt tggcgaaagt
     4801 caacaaaaaa gatatggaaa tcgccgccct gttgaaaaaa caccatcccg acctgtatgc
     4861 cgagttgtcc aaacagacca agtccgaaag aaaaaaacaa agttaaaccg caacctccgg
     4921 atgcccgaca gtttttcatt tccgaaaaac gcaaacaatg ccgtctgaaa caccggacag
     4981 gtcgccgtat cccgcctgcc gcccctgcct caaaccgccg aaccgcccga acccgccttt
     5041 ttacaaactt tatccaattt cctgtttatt tcgggatacg ccgacattag aatgtcaaac
     5101 agctcgaaac gggcaaactc cacatccatc caaaggaata aaaatgaaac ttctgaccac
     5161 cgcaatcctg tcttccgcaa tcgcgctcag cagtatggct gccgccgctg gcacggacaa
     5221 ccccactgtt gcaaaaaaaa ccgtcagcta cgtctgccag caaggtaaaa aagtcaaagt
     5281 aacctacggc ttcaacaaac agggtctgac cacatacgct tccgccgtca tcaacggcaa
     5341 acgcgtgcaa atgcctgtca atttggacaa atccgacaat gtggaaacat tctacggcaa
     5401 agaaggcggt tatgttttgg gtaccggcgt gatggatggc aaatcctacc gcaaacagcc
     5461 cattatgatt accgcacctg acaaccaaat cgtcttcaaa gactgttccc cacgttaatc
     5521 aggcaacaaa aaacagcgtt ttcagaaatg aaaacgctgt ttttttgacc gttccattat
     5581 tcacaaaagg gaaaaaacga ttacctgccc cgtgtatcaa aacctgccct gccggatgaa
     5641 gggcataacc ggcagggacg gcgtcaacac catatggggg tacggctttt cttgaaagat
     5701 tcggcttaaa tatccaatac tttcgcggta taggcgataa tttcatccgc cctttcaggg
     5761 ttttcgttca acttgatgcc gtaacccggt accagctctt tcagacggtc ttcccaagac
     5821 ggggcgcgct cggggaagca ttggtgcatc agccggatca tcagcggcac agcggtcgat
     5881 gcgcccggcg acgcgcccag caatgcggcg agtgagccgt cggcgtgggc gacaatctcc
     5941 gtaccaaact ggagcacgcc gcctttttcg gagtcttttt taatgatttg gacgcgttgc
     6001 cctgcggtga tgagttccca gtcgtcgggg tttgcctcgg ggtagtattc cagcagggag
     6061 gcgaagcgtt cttctttggt tttacgcaat tcgcccagca ggtatttggt cagcggcata
     6121 ttcgcccagc cggcgcacag cataggatag aggttgtcca tatggatgga cagcggcaaa
     6181 tccataagcg agccttgctt gaggaagttg gaacggaagc ctgcgtaagg gccgaacata
     6241 aggtggcgtt tgccgtccac gttgcgtgtg tcgaggtgcg ggacggacat cggcggcgcg
     6301 ccgacggaag cctgcccgta cactttggcg ttgtgttgtt cggcggtttc ggggttgctg
     6361 ttgcggaaga acaggccgga cacggggaag ccgccgtagc ctttgccttc ggggatgccg
     6421 gatttttgca gcagggtcag cgcgccgccg cccgcgccga ggaagaggaa gcgggtacgg
     6481 agggtgagct gcccgtcggg gttgcgggta tcggcggttt tgagcaccca cgcgccgtcg
     6541 gattcgcgtt tgatgtcttc gacgtggcgg ttgaactcgg tttttacgcc cttgccctgc
     6601 aaatatttca ccatttggcg cgtcagccgt ccgaaatcga catccgtacc ttcggcggag
     6661 tagttggcgg cgacgggttg gttttcgtcc cggccgcgca tcatcagcgg agcccaatcg
     6721 gaaattttgt tccgatcggt ggaaaattcc atattttcaa aaagtttttg ggttttaaac
     6781 gcgtcataac gtttttgaag ataagaacaa tggtcttcat tcatcaccaa agacatatgc
     6841 ggcacggcat tgatgaagga attgtcttcc aacttgcctt ccgcgaccag cgtcgcccaa
     6901 aactggcggc tgacatgaaa ctgttcggca atattgaggg cgcgcgccgg atcgataatc
     6961 ccatttgcac ccaacggcgc atagttcaat tcgcacagcg cggaatgccc cgtgccggcg
     7021 ttgttccacg cgtttgacga ttccaacgcc acatcttcca agcgttcaat cagggtgatt
     7081 tcccaagacg gttcgagttc tttgagcaaa acgcccaaag tcgcgctcat aatgccgccg
     7141 cccaccaaga caacgtctgt cgcttcagcc atggtttact cctaaaaaac aggcatcttc
     7201 tgcccttatg gttatttgcc gtactacaaa cgcctgaatc gcaaaagcag ggaaaaccgg
     7261 caatggtgtg tgtccgagta tgctgtttcg gggttggaat gcgttgcaag catggcttcc
     7321 gacaccgctt caggggcttg taatatgtta tcgtgaatgt agtggatttt actgggaaat
     7381 gcaaagtttt tctgtcgccc gccaagtcgg gaaactgcga aatgaaaaat aaaaatagtt
     7441 atttatctat atatatcaaa tttttaatag ataaaaaatc aaaattgttt atatattaat
     7501 ttttaaaaga ttgtcagcat attgcgt
//
LOCUS       DAWGRZ010000100         7501 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000100 DAWGRZ010000000
VERSION     DAWGRZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7501
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.100"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            125..1069
                     /gene="tehA"
                     /locus_tag="WHT44_001418"
     CDS             125..1069
                     /gene="tehA"
                     /locus_tag="WHT44_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HEZ2533592.1"
                     /translation="MSASKKFPIPLSYFSIALGLFALGLSWRYGASVGLLPALAAESL
                     LAAASVVWLLLVAAYLIKMFAYRNDFLSDLRDLVQCCFISAIPITAMLEGLALKPYQA
                     GAAAVLIYVGVAGQLAFSMYRAAGLWRGLHSLEATTPIIYLPTVATNFVSASSLAALG
                     HHDYAALFFGAGMFSWLSLEASILGRLRTAAPVGTAARGVVGIQLAPAFVGCGAYFAV
                     GGKVDGFALALIGYGCLQLLFLLRLTRWFWEGGFTMSFWGFSFGFAAMAGCGLHLAAS
                     GVLSGLGLTLATAGSAGVALLLVGTLHRIATGRFLVRS"
     gene            complement(1142..1825)
                     /locus_tag="WHT44_001419"
     CDS             complement(1142..1825)
                     /locus_tag="WHT44_001419"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ2533593.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            1973..4276
                     /gene="parC"
                     /locus_tag="WHT44_001420"
     CDS             1973..4276
                     /gene="parC"
                     /locus_tag="WHT44_001420"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ2533594.1"
                     /translation="MNTQAHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPPHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLSDENEKRKLIVKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDCLKQTLEGRTV
                     LPVVILDSSGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SSGYGFITKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSELEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGRLLPISGSLKQLSSPK"
     gene            4296..5813
                     /locus_tag="WHT44_001421"
     CDS             4296..5813
                     /locus_tag="WHT44_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEZ2533595.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYVWLAIACWLIFFSIIHPHWQWQSLKMPRFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVVCSCLLSYGRYPLLYSSYAAILLIFNAIADGDIGKYPLISDAR
                     TASATFILVAASYLSAIFTSLSVKYIDRAGKLAYDSHIAYHRIKGLSQTVLERVQEAV
                     VVINAEGLAVLFNRKAKDLFPALEIGRRAGLSDSAAELWDQASPHTFEYVLGTPGLNA
                     GIRAVPVNKGSDKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIRHA
                     NDLLRENMEAGAADPFNAKLCKIIDGNICRIDKMLEDISSLNKRNKTERETIGLIPFW
                     EEFKQEFLLGHPDAADCIRPDIQGGSPTAYFDPAHLRQIMWNLANNAWRHSRKQPGSI
                     SVTIRPAQKNTVCILFADRPKCRNTCSNPFTPRRKTAPASGCMSPANWRTPISAI"
     gene            5865..6332
                     /locus_tag="WHT44_001422"
     CDS             5865..6332
                     /locus_tag="WHT44_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ2533596.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            6436..7188
                     /locus_tag="WHT44_001423"
     CDS             6436..7188
                     /locus_tag="WHT44_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ2533597.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQNTAAHINLHFGNAAEQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQDPAYQYTGKSTRFDVYLPYGTDKG"
BASE COUNT         1674 a         2260 c         1978 g         1589 t
ORIGIN      
        1 tcacttggtg cttcagcacc ttagagaatc gttctcttcg agctaaggcg aggcaacgtc
       61 gtactggttt ttgttaattc actatatctg tgcgttacga cggcatgccg tctgaagggt
      121 gtttatgtct gcatctaaga aatttccgat tcctttgagc tatttcagca tcgcgctggg
      181 cttgtttgcc ttggggctgt cgtggcgtta cggcgcgtct gtcgggctgc tgcccgcctt
      241 ggccgccgaa tcgctgcttg cggcggcttc ggtcgtctgg ctcttgctgg tggcggcata
      301 cctgatcaaa atgtttgcgt accgaaacga ttttttgtct gatttacgcg acttggtgca
      361 atgctgcttc atcagcgcga ttccgattac cgctatgctg gagggactcg cgctgaagcc
      421 ctatcaggca ggcgcggcgg cagtcctgat ttatgtcggc gttgccggac agttggcttt
      481 ttcgatgtat cgggcggccg gtctgtggcg cggcctgcat tccttggagg cgacgacgcc
      541 gattatttat ctgcctacgg ttgcgacaaa ctttgtcagc gcgtcatctc tggcggcgtt
      601 ggggcatcat gattatgcag ctttgttttt cggcgcgggt atgttttcct ggctgagctt
      661 ggaagcctcc atcttgggca ggctgcgcac ggcggcaccg gtcggcacgg cggcgcgcgg
      721 cgtggtcggc atccagcttg cgcccgcctt tgtcggctgc ggcgcgtatt ttgccgtcgg
      781 cggtaaagtc gacggttttg cgttggcatt aatcggctac ggctgcctgc agcttttgtt
      841 cttgctgcgc ctgacccgct ggttttggga aggtggtttt acgatgagct tttggggatt
      901 ttcattcggt ttcgcggcaa tggcaggatg cggtctgcat ctggcggctt ccggcgtatt
      961 gtcgggcttg gggctgacgc ttgccaccgc cggatcggca ggcgtggcgc tgctgcttgt
     1021 cggtacgctg caccggatag cgacggggcg tttcttggta cgcagctgat gcgttttgcc
     1081 gccttgtcaa aaatgccgtc tgaaacgctg ggattcagac ggcatttttt atttcacacc
     1141 cttacaggta gaatttttcg atgactttca aattgtcgtc caatttgtac accaacggct
     1201 gaccggtcgg gatttccaag cccataatgt cttcgtcgga aatgccctcg atgtgttttg
     1261 ccagcgcgcg cagggagttg ccgtgcgccg ccaccaagac gcgtttgccg ctcaaaatcg
     1321 cgggggcgat ttggtcttcc caaaacggca atacgcgctc cagcgttact ttcaggtttt
     1381 cgccgtcggg tacgacatcg gcaggcagat gggcatagcg gcggtctttg tgtgcggaaa
     1441 actcatcgtc tttgtccaaa agcggcggca gggtgtcgta gctgcgccgc cagatgcgga
     1501 cttgctcgtc gccgtattgt tcggcggttt gttttttgtc caggccttgc agttggccgt
     1561 agtggcgttc gttcagccgc cacgttttga tttgcggtac gaacagttgg tcggattctt
     1621 ccaaaacgat gttgcaggtc ttaatcgcgc gggtcaggac ggatgtgaag gcgatgtcga
     1681 actcatagcc gttttctttc agtttcttgc cggcggcggc agcctcggca agcccctgct
     1741 cgctcagctt cacgtcgcgc cagcctgtaa acaggttttt cgcgttccat tcgctttgtc
     1801 cgtggcggat aaataccagt tccatatcgt ctccaatgtg tgaaagtggg aaagccttat
     1861 ttataacata ttttcacatt tcccgtattt gattcagatt cagacacgcg cccactatgg
     1921 tttgccgttt tgatttacaa taatgtcctt tgctttacat tccgcataca caatgaatac
     1981 gcaagcgcac gccccacata ccgattccaa tacgctgatg ctcggccgat acgccgaacg
     2041 cgcctatctc gaatacgcca tgagcgtggt caaaggccgc gcgctgcctg aagtttcaga
     2101 cggccagaag cccgtgcagc ggcgcatttt gtttgccatg cgcgatatgg gtttgacggc
     2161 gggggcgaag ccggtgaaat cggcgcgcgt ggtcggcgag attttgggta aataccaccc
     2221 gcacggcgac agttccgcct atgaggcgat ggtgcggatg gcgcaggatt ttaccttgcg
     2281 ctatccctta atcgacggca tcggcaactt cggctcgcgc gacggcgacg gggcggcggc
     2341 gatgcgttac accgaagcgc ggctgacgcc gattgcggaa ttgctgttgt ccgaaatcaa
     2401 tcaggggacg gtggattttg tgccgaacta cgacggcgcg tttgacgaac cgctgcacct
     2461 gcccgcccgc ctgcctatgg tgttgctcaa cggcgcgtca ggcattgcgg tgggcatggc
     2521 gaccgagatt ccgccgcaca atttgaacga agtgacgcag gcggcgattg cgttgttgaa
     2581 aaagccgacg ctggaaaccg ccgacctgat gcaatatatt cctgcccccg attttgccgg
     2641 cggcggtcaa atcatcacgc cggcggacga attgcgccgg atttatgaaa ccggcaaggg
     2701 cagcgtgcgc gtgcgtgcgc gttatgaaat cgaaaaattg gcgcgcggac agtggcgcgt
     2761 catcgtaacc gagctgccgc cgaacgccaa ttccgccaaa atccttgccg aaatcgaaga
     2821 gcaaaccaac ccgaaaccga aagcgggtaa gaaacagctc aaccaagacc agctcaatac
     2881 caaaaagctg atgctggatt taatcgaccg cgtgcgcgac gagtccgacg gcgaacatcc
     2941 cgtgcgactg gtattcgagc cgaaatccag ccgcatcgat accgatacct tcatcaacac
     3001 gctgatggcg caaacttcgc tggaaggcaa tgtgtcgatg aacttggtga tgatgggttt
     3061 ggacaaccgc cccgcgcaga aaaacctgaa aacgattttg caggaatggc tggatttccg
     3121 caccgtaacc gtaacacgcc gtctgaaatt ccgtttgaac caagtggaaa aacggctgca
     3181 catcctcgaa ggccgtctga aagtctttct gcacatcgac gaagtgatta aagtcatccg
     3241 cgaatcagac gacccgaaag ccgatttgat ggcggcgttc gggctgaccg aaatccaagc
     3301 cgaagacatt ttggaaatcc gcctgcgcca gttggcgcgt ttggagggtt tcaaactcga
     3361 aaaagaattg aacgagttgc gcgaggaaca aggccgtctg aacatccttt tgagcgacga
     3421 aaacgaaaaa cgcaagctga ttgtcaaaga gatgcaggcg gatatgaaac aatacggcga
     3481 cgcgcgacgc acgctggtgg aagaggccgg acgcgccgtg ctgacgcaga ccaccgccga
     3541 cgaacccatc acgctgatcc tgtcggaaaa aggctggata cgcagccgcg ccggacacaa
     3601 tctcgatttg agccaaaccg cgttcaaaga aggcgactgc ctcaaacaaa ccctcgaagg
     3661 cagaacggtt ttacccgtcg tcatcctcga ttcatcgggc agaacctaca cgctcgatgc
     3721 cgccgaaatc cccggagggc gcggcgacgg cgtaccggtt tcctccttaa tcgagctgca
     3781 aaacggcgcg aaacccgttg cgatgttgac aggattgccg gaacaacatt atttattatc
     3841 aagcagcagc ggctatggct tcatcaccaa gctgggcgat atggtcgggc gcgtgaaagc
     3901 gggcaaagtg gtgatgaccg cagacagcgg cgaaaccgtt ttgccgccgg ttgccgtcta
     3961 tgcctcctcg ttcatcaacc ccgactgcaa aatcattgcc gccaccagtc aaaaccgcgc
     4021 cctcgccttc cccatcggcg aattgaaaat tatggcgaaa ggcaaagggc tgcaaatcat
     4081 cggattaaac gccggcgaat cgatgacgca taccgccgtt tcttccgagc tggaaatcct
     4141 gattgaaagc gaaggcaggc gcggcgcggc gcacaaagac cgcatcccca tctccctgct
     4201 tgaggcaaaa cgcggcaaaa aaggcagact attgcccata tcgggcagcc tgaaacagct
     4261 ttcttcccct aaataaaccc ggttccgcac atattatggt gatttccaac ccccgcgaac
     4321 ttgaaaaact caaagaccgg attcccaatc tgatcaacat catccgcgtc gccatcgttt
     4381 ttccgctgat gattatgcac atcctcgggc tggaaaccgg cagccgtgcg aacctgcacg
     4441 cttcgtggac ggcgtgggcg ttttatgttt ggctcgccat tgcctgctgg ctgattttct
     4501 tttccattat ccatccgcat tggcaatggc agtcgctgaa aatgccgcgt ttcagcgcgg
     4561 tagcggacat cacgatgatc ggcgtgctga cctacctgtt cggcggcatc gattccggct
     4621 tcggcatcct gatcctgccc ttcgtcgtct gctcctgcct gctcagctac gggcgctacc
     4681 ccctgctcta ttccagctac gccgccatcc tgctgatatt caacgccatt gccgacggcg
     4741 atatcggcaa atacccgctc atatcggatg cccgaaccgc ctcggcaacc ttcatccttg
     4801 tcgccgcctc ctatctttcc gccatcttca cctcactgtc ggtcaaatac atcgaccgtg
     4861 ccggaaaact cgcctacgac agccatatcg cctaccaccg catcaaaggc ttgagccaaa
     4921 ccgtactcga acgcgttcag gaagctgtcg tcgtcatcaa tgccgaaggg ctggcggtgc
     4981 tgttcaaccg gaaggcgaaa gaccttttcc ccgcgctcga aatcggacgg cgcgccggtc
     5041 tgtccgattc tgccgccgaa ctgtgggatc aagcctctcc gcacactttc gaatacgtcc
     5101 tcggcacacc cggcctgaac gccggcatcc gcgccgttcc ggtcaacaaa gggtcggaca
     5161 agctgctcat cctctacatc cgcccgcaaa gcgaaattca ggcagaagcc ctgtccgtca
     5221 aacttgccgc gctcggacaa ctgaccgcca acctcgccca cgaaatccgc aacccgatgt
     5281 ccgccatccg ccacgccaac gacctgctgc gcgaaaatat ggaagcgggg gcggcagatc
     5341 cgttcaacgc caaattgtgc aaaatcatcg acggcaacat ctgccgcatc gacaaaatgc
     5401 tcgaagacat ttcctcgctc aacaagcgca acaaaaccga acgcgaaacc atcggcctga
     5461 taccgttttg ggaagaattc aaacaagagt tcctgctcgg ccatcccgat gccgccgact
     5521 gcatccgtcc ggacattcaa ggcggcagcc cgaccgccta tttcgatccc gcccacctgc
     5581 ggcaaattat gtggaacctc gccaacaacg cgtggcggca cagccgcaaa cagcccggct
     5641 cgatttccgt caccatccgc cccgcgcaaa aaaacaccgt ctgtatcctc tttgccgacc
     5701 gcccgaagtg caggaacacc tgttcgaacc cttttacacc acggcggaaa acggcaccgg
     5761 cctcgggctg tatgtcgccc gcgaactggc gcacgccaat ttcggcgatt tgacctacct
     5821 accggaagcc aaatgtttcg aactcacatt accggaaaaa accaatgact gaactgcaac
     5881 accccgtcct cgtcgtcgat gacgaaaccg acattctcga cctgatggaa atgaccctga
     5941 tgaaaatggg cttgcgcgtc cataccgcgt caggcgttgc cgaagccaaa aacaagctcg
     6001 acagccaacg ctattcgctc gtcctgaccg atatgcgtat gccggacggc tcggggctgg
     6061 aagtcgtcca acacatcaac agccgcctgc tcgatacgcc ggttgccgtc atcaccgcct
     6121 tcggcaacgc cgatcaggca caggaagcgt tgcgttgcgg cgcgttcgac cccgatacca
     6181 tgcagataca ggactatctc gaccaaatcg aacgcgacat catcgaacaa accctcaaac
     6241 aaaccgaagg caaccgcacg caggccgcca aacgcttggg catcagcttc cgttccatgc
     6301 gctaccgtat ggaacgcctc aacatcggct gacgacaaaa cggcatccgc accatctccg
     6361 cccacccgaa aaaatgccgt ctgaaacggc acgggaaagc gggttcgccc cacgcccgaa
     6421 cggacacaaa acaccatgac cgacatcctt attgacaaca ccgccaccga aaccgtccgc
     6481 accctgatac gggcattccc ccttgtgccc gtttcccaac cgcccgaaca aggcagttac
     6541 ctccttgccg aacacgatac cgtcagcctc aggcttgtcg gggaaaaaag cagcgtcatc
     6601 gtcgattttg cctccggcgc ggcacaatac cggcgcacaa aaggcggggg cgaactcatc
     6661 gccaaagccg tcaaccacac cgcgcacccc accgtttggg acgcaaccgc aggattgggg
     6721 cgcgacagct tcgtcctcgc ctcgctcggg ctggccgtta ccgccttcga gcaacatccc
     6781 gccgtcgcct gcctgctttc agacggcatc cgccgcgccc tcctcaatcc cgaaacgcaa
     6841 aacaccgccg cgcacatcaa cctccatttc ggcaacgccg ccgaacaaat gcccgcactt
     6901 gtccaaacac aaggcaaacc cgacatcgtc tatctcgacc ccatgtatcc cgaacgccgc
     6961 aaaagtgccg ccgttaaaaa agaaatgacc tacttccacc ggctcgtcgg cgaagcgcaa
     7021 gatgaagcgg cactcctgca taccgcacgc caaacagcaa aaaaacgcgt cgtcgtcaaa
     7081 cgcccccgcc tcggcgaaca ccttgccgga caagaccctg cctaccaata cacaggcaaa
     7141 agcacccgct tcgacgttta cctgccctac gggacggaca agggataacg cccataaaac
     7201 aagacaccga aaaatttgcc gttcttatgc aaacgagaaa ccggtttttg cgtttcgact
     7261 gttttggata agtcatcaca ccttaaagtt tgtcattccc acagaagtgg gaatccgatt
     7321 cattcagttt tatagtggtt taaatttaaa ccactatagt tgttttcgag tttcaggcaa
     7381 cttccaaacc gtcattccca cggaagtggg aatctagaaa tgaaaggcaa caggaattta
     7441 tcgtaaatga ctgaaaccga acggactaga ttcccgccta cgcgggaatg acggggcggg
     7501 c
//
LOCUS       DAWGRZ010000101         7469 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000101 DAWGRZ010000000
VERSION     DAWGRZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.101"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(16..954)
                     /locus_tag="WHT44_001424"
     CDS             complement(16..954)
                     /locus_tag="WHT44_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533598.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGMLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVGRLRIWANSADTEEVRQKRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIAEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            1156..2268
                     /gene="nadA"
                     /locus_tag="WHT44_001425"
     CDS             1156..2268
                     /gene="nadA"
                     /locus_tag="WHT44_001425"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ2533599.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETADRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYICRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPEAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKQFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKKKDVFNGMGPA"
     gene            2426..2755
                     /locus_tag="WHT44_001426"
     CDS             2426..2755
                     /locus_tag="WHT44_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEZ2533600.1"
                     /translation="MHWLFLTVAILSEVCGSSMLKLSGGFSKLWPSIGVVVSFSVCFW
                     ALSMTLKTMPLATAYAIWAGVGLVLTALVSVVFFGEKADFIGIVSIGLILLGVVLLNT
                     MSHMSGH"
     gene            2804..4312
                     /gene="nadB"
                     /locus_tag="WHT44_001427"
     CDS             2804..4312
                     /gene="nadB"
                     /locus_tag="WHT44_001427"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ2533601.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRAILSQGKPAIEWLLAQGVAFDR
                     NHNGLHLTREGGHTCRRIAHVADYTGEAVMQSLIAQIRRRPNIRVCERQMALDIQTES
                     GAACGLTVLDCRTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VGNLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQAGERFMPHYDRRAELAPR
                     DIVARAIAAEIAKQTQDFVSLDISHQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLCAQA
                     IADGQAFQAEPFQRPSESLSAEAGIFSDDLQNTFSRPVLQTFNQRHLGILRNDTGLRR
                     AIAQLQLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            complement(4490..5155)
                     /gene="pgmB"
                     /locus_tag="WHT44_001428"
     CDS             complement(4490..5155)
                     /gene="pgmB"
                     /locus_tag="WHT44_001428"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ2533602.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGISIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVSEAEFAELTRRKNDNYVEMIQAVKPEDVYPGILPLLEA
                     LRANGKKIALASASKNGPFLLERMGLTHFFDAIADPAAVAHSKPAPDIFLAAAEGVDA
                     DIRQCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSGTAELTYAYLQSVWEQ
                     SGR"
     gene            complement(5168..7426)
                     /locus_tag="WHT44_001429"
     CDS             complement(5168..7426)
                     /locus_tag="WHT44_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ2533603.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINAFNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFNVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGSQTDWQVSNSFESEVGSTPETFEKRVIVTTSRDYQSLEAVKAAGRAL
                     SEKIAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRNGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGTLSFAPFLPGAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLDGSHAAALEK"
BASE COUNT         1536 a         2041 c         2190 g         1702 t
ORIGIN      
        1 tcgtctgaaa acgggctaac ggctgcccag cggcagtccg atgtccgaaa tcagcctgtc
       61 gggcaggacg tcgtcgcgga agcggcaaag ctgcgcggga cggcctttgc tgcccgatac
      121 gccggtatcc gacggctcga tgaggttttg ctgctgaatc tggcggcgga agttttgctt
      181 gtgcagcaat ctgccgctga tggcttcgac gctgttttgc agttgcagca gcgtgaattc
      241 gggcggcatc agttcaaaaa tcacggggcg gtatttgatt ttggcgcgca ggcgagacag
      301 ggcggtcgcc agtacgcggc ggtggtcgtg gcgcatgggc tgccccgtaa gcgcgaagtc
      361 gaagtttgcc tgcggctcgg cggcttccgc tatcaggccg ctttcataca gcatttcata
      421 gcgttgcaaa acgtattctt ccgaccagtt ttccggttcg accccccagc acaaatgaat
      481 gcgcttgagc cgcttttggc gcacttcctc cgtgtccgcc gagtttgccc aaatgcgcag
      541 gcggccgacg acggcgtcgc gctgcccgcc gtcggtgcgc aagtcttccc acgggaaata
      601 gccgtagcag tcctgccatt tcgcatccgg gtgcaggatg ctgtcggctg cctcgcgcac
      661 cagccccaaa tagctgacgt acagcacggg catgccgtgt tcgttgcggc ggtgggtatc
      721 gacaaaggtg taaagctgtt ccacatagcc cataggctgc gaagtctgct tggcgaccca
      781 cagttttacg cctgcctgca aggaattgcg caggggggag agcgggccgt tgggcaggag
      841 catgccttgg gcgacggtca atacccgcag gccgccgtcg gtaacggcaa tcaatacggg
      901 aaccagctcg acgatgcttt gcggcggggc ttcggcttcg gggtaggcat ccatgctcgg
      961 ctttctgcgg gtgggaaagg ctttattgta aaccaaagcg gtttgtcgga acgaagcggg
     1021 tcggttttac ggtattgcaa acctgttttc agacgacctt gcatattata ctcaagttga
     1081 gcataatgta aaaacagttt gaaaaacaga cggcaaacct tcatgccgtc tgaaaaacag
     1141 ctacaaggaa acatcatgca aaccgccgcc cgccgctcgt tcgactacga tatgccactc
     1201 atccaaacgc cgacttccgc ctgccaaatc cgtcaggcgt gggcgaaggt tgccgacacg
     1261 cccgaccgcg agacggcaga tcgtctgaaa gacgaaatca aggctttgct gaaggagaaa
     1321 aacgcggtct tggtggcgca ttattacgtt gatccgctga ttcaggattt ggctttggag
     1381 acgggcggat gcgtgggcga ttcgctggaa atggcgcgct tcggtgcgga acacgaagcc
     1441 ggtacgctgg tggtggctgg tgtgcgcttc atgggcgaga gcgcgaaaat cctctgccct
     1501 gaaaaaacgg tgctgatgcc tgatttggag gcggaatgtt ctttggattt gggttgcccg
     1561 gaagaagcgt tttcagcgtt ttgcgaccaa caccccgacc gcacggtggt ggtgtacgcc
     1621 aacacttccg ccgccgtgaa agcgcgtgcc gattgggtgg taacgtcttc ggtggcgtta
     1681 gaaatcgtat cgtatttgaa atcacgcggc gagaagctga tttggggacc cgaccgccac
     1741 ctcggcgact acatctgccg cgaaacgggc gcggatatgc tgttgtggca gggttcgtgc
     1801 atcgttcaca acgaattcaa agggcaagag ctggcggcgt tgaaggcgga acaccccgaa
     1861 gcggtggtgc tggttcatcc tgaatcgccg caaagcgtca tcgaactggg cgacgtggtc
     1921 ggctcgacca gcaaactgct caaagccgcc gtatcgcgtc ctgaaaaaaa attcatcgtg
     1981 gcgaccgatt tgggcatcct gcacgaaatg caaaagcagg cgcccgacaa acaatttatc
     2041 gccgcgccga cggcgggcaa cggtggaagc tgtaaaagct gcgcgttctg cccgtggatg
     2101 gcgatgaatt cgctgggcgg catcaaatac gccctgacaa gcggacgtaa cgaaatcctg
     2161 ttggacagga agctgggcga agccgccaaa ctgcctttgc agcgtatgct cgactttgcg
     2221 gcagggctga agaaaaaaga tgtgttcaac ggcatgggcc ccgcctgatt tgtcgttcgt
     2281 aatgggtgca gcctgaaaat accgcccgcc tgctttccca agacgggatt tgcacacaaa
     2341 aaatctttaa caacaagact gtttttttat tttattgcat tgatataatt atactttctt
     2401 tcatttatca ggtaaaaaca tgcaaatgca ctggctcttt ctgactgtag caattttaag
     2461 cgaagtctgc ggttcttcca tgctcaaact gagtggcggg tttagcaaac tgtggccttc
     2521 tattggcgtg gtagtcagct tttcggtgtg tttttgggcc ttgtctatga cactgaaaac
     2581 catgccgctg gctacagcat acgccatttg ggcaggcgtg ggactggttt taacggcttt
     2641 agtcagcgtg gtgtttttcg gtgagaaagc tgatttcatt gggattgtca gcatcggttt
     2701 gattttgctg ggcgtggtgc ttctcaatac catgtcgcat atgtcggggc attgattaat
     2761 ccactatacc gtttcagacg gcctctcaaa caaggacaac accatgcaaa ccgattgcga
     2821 cgtattgatt gccggaaacg ggctggcggc actgacgctc gccctgtcgc tgcctgaatc
     2881 gttccgcatc gttattttgt gcaaaaaccg gctggacgac accgccagcc gtcatgcgca
     2941 aggcgggatt gcggcggcgt ggtcgggaga ggacgacatc gaaaaacacg ttgccgatac
     3001 tttggaagcg ggcgcgggtt tgtgtgatga agccgccgtc cgcgccatcc tgtcgcaggg
     3061 caaaccggca atcgaatggc tgctggcgca gggcgtggcg ttcgaccgga atcataacgg
     3121 cctgcacctg acgcgtgaag gcgggcatac ctgccgacga atcgcccacg tcgccgacta
     3181 cacgggcgaa gccgtcatgc agagcctgat tgcccaaata cgccgccgcc cgaacatccg
     3241 cgtttgcgag cggcagatgg cgttggacat tcaaaccgaa tcaggcgcgg cgtgcggact
     3301 gaccgtcctc gactgccgaa cacaagaaac ctaccgcatc cgcgcccgcc ataccgtact
     3361 cgcaggcggc ggcttgggac agatttacgc cgccaccacc acgccgcccg aatgcacggg
     3421 cgacgccatc gccatggcga tacgcgcagg ctgcgcagtt ggaaacctcg aatttatcca
     3481 attccacccc acaggcttgg caagaccgtc tgaaaacgga cgcaccttcc tcatttccga
     3541 agccgtgcgc ggcgaaggcg gcatcctgac caaccaagcg ggcgaacgat ttatgccgca
     3601 ttacgaccgc cgcgccgaac tcgcaccgcg cgacatcgtt gcccgcgcca tcgcagccga
     3661 aatcgccaaa caaacgcaag acttcgtctc gctcgacatc agccatcaac ccgcagcgtt
     3721 cgtccgccgg catttcccat ccatccatcg acactgttta tcccaatgcg gcctagacat
     3781 cacgcgccaa gccatccccg tccgccccgt gcaacactac acctgcggcg gcatccaaac
     3841 cgacccctgc ggcagaacct ccctgccgca gctctacgcc ttgggcgaaa ccgcctgcac
     3901 tgggctgcac ggagccaacc gccttgccag caactccctg ctcgaatgcg tcgtgaccgc
     3961 aaggctctgc gcccaagcca tcgcagacgg acaagcattc caagccgaac cattccaaag
     4021 gccgtctgaa agcctctccg ccgaagcagg catcttttca gacgaccttc aaaacacatt
     4081 cagccgcccc gtcctgcaaa cgttcaacca acgccatcta ggcatcctac gcaacgatac
     4141 cggcctgcgc cgcgccatcg cccaactgca gctttggaag caaaaccaag ccgaaccgca
     4201 caccgcgtcc gaatacgaaa accgcaacct gctcgaatgc agcctcgccg tcgcccaagc
     4261 cgcatacagg cggagacaga acatcggcgc gcattttaat agtgattgtt aaggttggct
     4321 ggttagaaaa aaaatcgttc aaataaattt gaatagcatg gttaagagat tggcatttag
     4381 tttttagggt tgcgtaagtt gggttatcat tctgttctaa aagcaaaaag gccgtctgaa
     4441 acactgttca gacggtcttt tgcttccttg acactttatc tgacgcgttt tacctgcccg
     4501 actgttccca cacgctttgc aggtaggcgt aggtcagctc ggcggtgccg gagaccagcg
     4561 cgatgtcgct gcccaagtct tcggctttgc ccacgccgat gggcaaggcg ccggcggctt
     4621 tgatggcggc gacgccggcg gcggcgtctt caatgccgat gcattggcgg atgtccgcat
     4681 ctacgccctc ggctgctgcg aggaagatgt cgggggcggg tttggaatgt gcgacggcgg
     4741 cagggtcggc aatggcgtcg aagaagtggg tcagccccat gcgttccagc aggaacgggc
     4801 cgtttttact ggcggacgca agggcgattt ttttgccgtt tgccctcaat gcttccagca
     4861 ggggcaaaat gccgggatac acgtcttcgg gtttgactgc ctgaatcatc tcgacgtagt
     4921 tgtcgttttt acggcgggtc agttcggcga actcggcttc gctgacggtt ttgccgccgt
     4981 gcgcgaggat gcgtttgagc gaatcgtcgc gcgacacgcc tttgagctgc tcgttaaact
     5041 tgcggtcaat gctgatgccc agttcttcgg cgagcttttt ccatgcgcgg tagtggtatt
     5101 cggcggtgtc ggtgatgaca ccgtcgagat cgaagaggac tgcggtaaac gtcattttgc
     5161 accctcctta tttttccaac gcggcggcgt ggctgccgtc gagcgtgatg tctttgccat
     5221 acacctgcaa atcgagcggc tcgcctttga gcagggtgaa gacgacgttt tctttgccga
     5281 cggcgacttt aatcagacgg cctcggtagt tgatatggaa ggcgtagcct gtccatgcgc
     5341 ccggcaggaa cggtgcgaag ctgagtgtgc cgccccaggt tttcatttgg gcgaaacctt
     5401 ggacgatggc gagccacgag ccggtcatag aagtaatgtg caggccgtct tcggtgtcgt
     5461 tgttgtagtt gtccaagtcc aggcgtgcgg tgcgccggta catttccacg gctttttctt
     5521 ctttgcccag ttcggcggcg agaatagagt ggatacacgg cgacaacgag ctttcatgca
     5581 cggtcatcgg ttcgtagaag tcgaagttgc ggcgtttttc gtcgatattg aaacggtcgc
     5641 cgaagaagta gatgccttgc aagacgtccg cctgtttgat gaagggcgaa cgcaggattt
     5701 tgtcccacga ccatttctgg ttgagcggca aatcgtcggg cgaaagcgcg gacactgggc
     5761 ggatgtcttt atcgaggaag ccgtcgtgct gcacgaatac gccgagttct tcgtcatgcg
     5821 gacggtacat attcgcgctg atgtccgccc atttttccaa ctcggcggca cgcacgttca
     5881 aatccggacg cgggtatttc gccaaggctt cgcgggtgta gtctaatacc catgcggcga
     5941 gggtgttggt gtaccagttg ttgttgatgt tgttttcgta ttcgttcgga ccggttacgc
     6001 cgtgaatcat gtatttgccg ttgcgtttgg agaagtggac gcggtccgcc cagaagcggg
     6061 acacttcgac caagacttcc aagccttctt tagcaagata gccctcgtcg ccggtgtagt
     6121 tggtgtagtt atagatggcg taaggaatcg cgccgttgcg gtggatttcc tcgaaggtga
     6181 tttcccattc gttgtggcac tcgatgcccg taaacgttac catcggatag agtgcgcccg
     6241 ccaagccctg ttcgcgcgcg ttgtgttgtg cctgcggcag ttggttgcgg cggtattgca
     6301 gcaggttgcg ggtaacttcg ggttctgcca gtgcgaggta gagcggtacg gcgtaggctt
     6361 cggtatccca ataggtcgca ccgccgtatt tttcgccggt aaagcctttc gggccgatgt
     6421 tcagtcgcgc gtcttcgccg tagtaggtgg agaacagttg gaacaggttg aagcggatgc
     6481 cctgctgtgc ttcgtcgctg ccttcgatga ccacgtcggc gatttcccaa cggtgcagcc
     6541 agcctgcttt gtgcgcgtcc agcaaggttt caaacgcaac gcctgcaatt ttttccgaca
     6601 aggcgcggcc tgcggctttc actgcttcca agctctgata atcgcggctg gtggtaacaa
     6661 tcacgcgttt ttcaaaggtt tcgggtgtgc tgccgacttc ggattcaaaa gaattggaga
     6721 cctgccagtc ggtttggctg ccgccgaggg ctttgaagct gccggcaaag gtttgctcgg
     6781 cgttgacgat gaattgttcc acgccgaagg gattggcgac ggtttgggcg gcaatgtagg
     6841 agagactgtc tgaaacgcct ttgtccaata cctgccagaa tttttcttcg tagttggagt
     6901 cttcgttttt cacgtcggca tcgatgatgg aatcgatgcg gacttggtgg gttttaccgt
     6961 caacggatac ggcttcccag cggatgaccg ccagctcttt ttgtgcgaca gacaggaatt
     7021 tgcacacatt gaaacgcaca ccgaatacgg tgaacgagcg gcgcaacacg ccgtgctgca
     7081 tatcgagttc gacggagaag ccagcaacgt cgtttttcgc caagtccact tcctgcccgt
     7141 cgacaaagat tttgactttg ctgaaattga acgcgttgat ggctttgccg aaatatttgg
     7201 gatagccgtt tttccaccag ccgacgcggg ttttgtcggg gaaccacacg ccggcgatgt
     7261 aggtgcctaa gtggctgtcg gcggaatagg tttcctcaaa gctgccgcgc atacccatat
     7321 agccgttgcc caagctggtc aggctctctt gcagccgttt gtgttctttt tccagttttg
     7381 ccgaacgcag cgtccaaggg ctgatttcca tgattcttgt gtacatttat gaagctcctg
     7441 tttggattga tttgagggaa tggtgaaat
//
LOCUS       DAWGRZ010000102         7339 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000102 DAWGRZ010000000
VERSION     DAWGRZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.102"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            66..260
                     /locus_tag="WHT44_001430"
     CDS             66..260
                     /locus_tag="WHT44_001430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533604.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKPYACTCNNPTDTA
                     VFSKPFASFTHPPRDAAATI"
     gene            431..955
                     /gene="nspA"
                     /locus_tag="WHT44_001431"
     CDS             431..955
                     /gene="nspA"
                     /locus_tag="WHT44_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ2533605.1"
                     /translation="MKKALATLIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSIGLGVLTGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSAGVRVKF"
     gene            1091..1480
                     /locus_tag="WHT44_001432"
     CDS             1091..1480
                     /locus_tag="WHT44_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ2533606.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            1558..2388
                     /locus_tag="WHT44_001433"
     CDS             1558..2388
                     /locus_tag="WHT44_001433"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ2533607.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRITKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLKGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            2403..3860
                     /locus_tag="WHT44_001434"
     CDS             2403..3860
                     /locus_tag="WHT44_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ2533608.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(3901..4290)
                     /locus_tag="WHT44_001435"
     CDS             complement(3901..4290)
                     /locus_tag="WHT44_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533609.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDIPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLF"
     gene            complement(4472..4873)
                     /locus_tag="WHT44_001436"
     CDS             complement(4472..4873)
                     /locus_tag="WHT44_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533610.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(5290..5619)
                     /locus_tag="WHT44_001437"
     CDS             complement(5290..5619)
                     /locus_tag="WHT44_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533611.1"
                     /translation="MTDKSKTEKLISSDDKQSVIDGILDMVFNSKAYEVPWISEKLME
                     LSKNKDLDIAGLSLTCFGHLARLHSNIGDYDKVIPLLHSKQDDPELQGRAEDALEDIS
                     LFLSENH"
     gene            complement(5645..6352)
                     /locus_tag="WHT44_001438"
     CDS             complement(5645..6352)
                     /locus_tag="WHT44_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ2533612.1"
                     /translation="MVKTADGYKAIARIRTGDRVFAKDEASGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNNQTLISNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITF
                     KQQPLKAYNLTVADWHTYFVKGSQAETEGVWVHNDCPYDKGNQRYKDASYHGKNDNSV
                     KSRAPTNGQAALDNSVQVKSTSPRRVGVDKANNEIVVLNKTQTFNNGSAEYHGHVRSW
                     QDLHTDQKNALKKAGLD"
     gene            complement(6487..6804)
                     /locus_tag="WHT44_001439"
     CDS             complement(6487..6804)
                     /locus_tag="WHT44_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533613.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            complement(6807..>7339)
                     /gene="mafB"
                     /locus_tag="WHT44_001440"
     CDS             complement(6807..>7339)
                     /gene="mafB"
                     /locus_tag="WHT44_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ2533614.1"
                     /translation="DQWMQENPNAAETVEALVNVLPFAKVKNLTKAAKPGKAAVSGDF
                     SDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGAQGKHISGHRNYIEGKST
                     LNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSDGKSGLSTNFGTIHSGKN
                     GVHIVPANPKTIKKVQ"
BASE COUNT         1820 a         2025 c         1512 g         1982 t
ORIGIN      
        1 cgcaaacaaa tcggcggccg cccgggaaaa cccgccccga acgcgtccgg aaaatatgct
       61 tatcgatgga aaacgcagcc gcatcccccg ccgggcgttt cagacggcac agccgccgcc
      121 ggaaatgtcc gacgcttaag gcacagacgc acacaaaacc gtatgcctgc acctgcaaca
      181 atccgacaga taccgctgtt ttttccaaac cgtttgcaag tttcacccat ccgccgcgtg
      241 atgccgccgc caccatttaa aggcaacgcg cgggttaacg gctttgccgt cggcaaagca
      301 gccggatacc gctacgtatc ttgaagtatt gaaaatatta cgatgcaaaa agaaaattta
      361 agtataatac agcaggattc tttaacggat tcttaacaat ttttctaact gaccataaag
      421 gaaccaaaat atgaaaaaag cacttgccac actgattgcc ctcgctctcc cggccgccgc
      481 actggcggaa ggcgcatccg gcttttacgt ccaagccgat gccgcacacg caaaagcctc
      541 aagctcttta ggttctgcca aaggcttcag cccgcgcatc tccgcaggct accgcatcaa
      601 cgacctccgc ttcgccgtcg attacacgcg ctacaaaaac tataaagccc catccaccga
      661 tttcaaactt tacagcatcg gcgcgtccgc catttacgac ttcgacaccc aatcgcccgt
      721 caaaccgtat ctcggcgcgc gcttgagcct caaccgcgcc tccgtcgact tgggcggcag
      781 cgacagcttc agccaaacct ccatcggcct cggcgtattg acgggcgtaa gctatgccgt
      841 taccccgaat gtcgatttgg atgccggcta ccgctacaac tacatcggca aagtcaacac
      901 tgtcaaaaac gtccgttccg gcgaactgtc cgccggtgtg cgcgtcaaat tctgatatgc
      961 gccttattct gcaaaccgcc gagccttcgg cggttttgtt ttctgccacc gcaactacac
     1021 aagccggcgg ttttgtacga taatcccgaa tgctgcggct tctgccgccc tattttttga
     1081 ggaatccgaa atgtccaaaa ccatcatcca caccgacaaa gcccccgccg ccatcggcgc
     1141 gtacagccaa gccgtccgag caggcgacac cgtttacatg agcggtcaaa tccccctcga
     1201 tcccgccacg atgaccgtcg tcggcaacgg cgatttccgc gccgaagcgc gccaagtgtt
     1261 ccaaaacctg caagccgtcg ccgaagcggc aggcggcacg ctggccgaca tcgtcaaact
     1321 caacgcctac ctgaccgact tgggcaattt tgccgtcttc aacgaagtca tggcggaatt
     1381 tatcgccgag cccttccccg cccgcgccgc cgtcggcgtt gcctcgctgc ccaaaggcgt
     1441 gcaggtcgaa gccgaagccg tcctcgtttt gaacgcataa ttccacgttc ggactgcatg
     1501 atatttcaaa accatgccgt ccgaaccgca acactttccc acttcacaac atctgcaatg
     1561 gcacattacg caatcggcga catacaaggc tgtttcgacg aactgaccgc gctgctcggc
     1621 aaaatcggtt tcaaccacgg cacggacacc ctctggctga cgggcgacat cgtcaaccgc
     1681 ggcccgaaat ccctcgaaac gctgcaattc tgcatccggc acgaaaacag cgtgcaaatc
     1741 gtcctcggca accacgacct gcacctgctc gctgtcggct gcggcgaagg tgcgcccaaa
     1801 cgcagcgaca caatcgaacc catactcaaa caccccgacg gaaaaaaaat gctcgactgg
     1861 ctgcgtgcgc aaccgctgtt gatacgcgag ggcagccgcg tgatggtaca cgccggcatc
     1921 ctgccgcaat ggcgcataac caaagccgaa tcgctcgcta gagaagccga agccgaactg
     1981 cgcggcaaaa aatacgtcaa attcttctcc aaaatgtacg gcaacaaacc ggccgcgtgg
     2041 gacgaaggtt tgaaaggcta tgcccgcctg cgcttcatcg tcaacgcctt cacgcggatg
     2101 cgcgccctga cctttaaaaa cgaactggat ttcgactaca aatccacagt gaaaaaaatg
     2161 ccgccttacc tgcgcccgtg gttcaaagcc cccgaccggc aaaacctcga ccgcaccatc
     2221 atcttcggac actggtcctc gctgggctac acgaatgccg acaacgtcat ctcgctggac
     2281 accggcgcgc tgtggggcgg acagctgacc gccgtcaacc tcgaaacgga agaaattacc
     2341 caagtccaag ccgccggcgg catagactgg aaaagcttcg caaaataacg gaccgcccca
     2401 ctatgcacaa aatcctgccc atcgcccacg tcctctcccg actgggtatg ctgttttcct
     2461 ttatcctgct gatacccgcc gccctctcct acgccttttc ggacggcgcg tacaccgcct
     2521 tcgccaccac cgcgaccgtt accctttccg gctcgtgcat cgtccggctc gccaccctcc
     2581 ggttcaggcg cgaactgcgc ccgcgcgacg gctttaccct cgtcctgatg ttgtggctgg
     2641 cgtttgccgc tatggctgcg atgccgatgt acctgtattt cccgaatatg ggctttaccg
     2701 acgcattttt tgaatcgatg tcgggactga ccaccaccgg cgcgaccgtc atcccccacg
     2761 tcgacgggct cgccccctcc gtcaactttt ggcggcatat gctcaactgg ctgggcggga
     2821 tgggcatcat cgtccttgcc gtcgccatcc tgcctatgct cggcgtaggc ggcacgcagt
     2881 tattcaaagc cgaaatcccc ggcattgaca aagaaagcaa aatgtcgccg cgcatttccc
     2941 aagtggcgaa aaaactttgg ttcggctaca ccctgatcac catcctcgcg gcagcctgcc
     3001 tgcattttgc cgggatgggc tggttcgatg ccgtctgtca cgcgatggct accctctcgc
     3061 tgggcgggtt ttccacccac gatgccagca tagcttatta caactccccc ctcatcgaag
     3121 cggtcatcat cgttttcacc attgtcggcg gcatcaattt tgccaaccat ttcgccgccc
     3181 tcaacagccg cagcctcaaa acctattgga aagacgaaga atgccggacg atgctgctgc
     3241 tgctctccgg cagcatcctt gccgccgccc tgtacctgtg gcacaccggc tattacgccg
     3301 gttttaccga atccctgcgc tacaccgcct tcaacttcgt ctccatcgga ctggcaaacg
     3361 ggctggccaa caccgacttc gcacaatggc cgctcctgat ttccctgtgg atgtttttcc
     3421 tcgccaacat cctcgccaac tccggctcga ccggcggcgg catcaaaacc atacgcgccc
     3481 tcgtcctgtt caaattcagc ctgcgcgaaa tgatggtgct gctgcacccc aaagccgtcc
     3541 gcaccgtcaa aatcagcggc aaggccatac ccgaccgcct cgcgctgacc gttatgtcct
     3601 tcatcttcat ctactttatg accgtcgtcc tcttcagctt cctgctgatg gcgagcggta
     3661 tggaatttac caccgccttc accgccgtca tcgcctgcat caccaacgcc ggccccggcc
     3721 tgggcgaagt cgggcccgcc ggcaattacg ccggtttgga cgtgatgcag aaatggatct
     3781 gcgttaccgc catgctcttg ggcaggctgg aaatcttcac cgtcttcatc ctcttcaccc
     3841 ccgcctactg gaagaaataa aaaatgccgt ctgaaaactt ttcagacggc atattgtctt
     3901 ttaaaaaagc ggaagctcta caaaaggaaa gaaatgctga aacctctgat aaatttcagg
     3961 atatttctct aaggctttta atgctttttc ttttgaaata ggcggatcat agacatctat
     4021 cccccttaag aaggcaatgc ccgttaatgc atttttttga tcatttgaaa tgtaaccata
     4081 attaacgact ccaattacat cataaatttc ccctatactc ttatcaaaat caatcaaatc
     4141 aaaaatatac aatggaattg tatcaatagg aatatctctg acaactatat ccaaccataa
     4201 tttaaattca gaaagattaa tagcatcata gaacatacag actattgcaa atcctaaatc
     4261 ttcacaattt tctttactaa ttttccacat aattatttac ctttatccat tgcttcccaa
     4321 ccgatacatc cggtatttta gcttctcctt ttccgtcaaa cactcctttg gttacctgct
     4381 ttgtgtcaaa aataccgtct gaaagccgaa tatcgtttca gacggtattt tgattgttgg
     4441 gtttttaatc agagctttgt ttgataattt gttattttga ataaaataca ggttctgtcg
     4501 agctgataac ttctattaca gaatcgctaa aaaagaaaat ataagcctta taatatttct
     4561 tttcaaaaaa atcaaaactt tcttctttca accagtccat ataactatca tctattatct
     4621 cgaaaaaata atttatagca ggatttctat cccaatcatt tttttcttta ttttcaaata
     4681 aatattctga aaattttact ttataagatt catccgttat ttgaaaatga attatattgt
     4741 caataaatat ttcaatattt aattcataat ttcggtcgga tgccaaaatg ttcacatctt
     4801 gttcggtaac acaaattttg tccacataaa atctgtgatt attgttagaa aattgtaatg
     4861 gtttaatctt cacaatcgtt ttccatatct aatttttgtt ttcttattgc cctgccggat
     4921 aaagcaggtc tgcctccgta ctgtctatcc tcacatttac tttcctgcca tcggctttta
     4981 tctccccggt tttaccgttt ttgccaactt tgcggctttg gcggcggcaa tgttgaaggc
     5041 ggcttcgacg gtttcggcag cattggggtt gacccgtact catccgcaaa cggcaacacg
     5101 acggacggtg tttgcttttc agccgtctgc cgttttgacc aaggtgctgc cgtggaagga
     5161 gcaagtgtag gatttttgaa gaactcagtt ctcaatatcc tatttcaaat gaaagaaatg
     5221 ccgtctgaaa attaaacacg gtttcagacg gcattttgat tgttgggttt tcaacccgac
     5281 ctacggttcc taatgatttt cagataaaaa taaagaaata tcttctaacg catcttcagc
     5341 cctaccttga agctctggat catcttgctt tgaatgtagt aaaggaataa ctttatcgta
     5401 atcaccgata tttgaatgta gcctggcgag atgtccgaaa caggttagcg ataatccggc
     5461 aatatccaag tctttatttt tcgataattc catcaatttc tcagaaatcc acggtacttc
     5521 atatgctttg gaattaaata ccatatcaag aatgccatct ataacacttt gtttatcatc
     5581 agaagaaatc aacttttctg ttttactttt atcagtcatt ttttaatttt tccttttgaa
     5641 ttaactaatc caatcctgct ttttttaaag catttttctg atcggtatgc aaatcttgcc
     5701 aacttctgac atgcccgtga tattccgcag aaccgttatt aaaagtttga gttttgttta
     5761 atacaacgat ttcattattg gctttatcaa ccccaactct tcgaggagaa gttgatttaa
     5821 cttgaacgga attatcaaga gctgcttgtc cgtttgttgg tgctctactt ttcacagaat
     5881 tatcattttt gccatgataa gaagcgtctt tatatcgttg gttgccttta tcatacggac
     5941 aatcattatg aacccaaacc ccttccgttt ccgcctgact gcccttgacg aagtaggtat
     6001 gccaatcggc gacggtcaga ttgtaggctt tgagcggctg ctgtttgaag gtaatgtttt
     6061 gaaccgtctg ttttgcgccg ctttcggaaa gcagggtgtc gccttttttc agacgacctg
     6121 cctgtatcca ttttccttga ctgtaaaacg ggtggatttt attggaaatc agggtttggt
     6181 tgttgccgat gccgtctgaa atttcaatgt aaacggtttc ttgatacgga ttgccgtatc
     6241 gggcggtaac gggtttgtat cccgtttttc cgcttgcctc gtccttggcg aagacgcggt
     6301 cgccggttcg gatacgggca atggctttgt agccgtctgc cgttttgacc aaggtgctgc
     6361 cgtggaagga gcaggtgtag gatttttgaa gaactcagtt ctcaatatcc tatttcaaat
     6421 gaaggaaatg ccgtctgaaa attaaacacg gtttcagaag acattgtgat tgttggtttt
     6481 tttaacctac gcttgcgaaa ttaattcctt tctcctgaat actgcaccat ttatttggtt
     6541 taactccata gaagaatgat tagaataaat acattcaaag tcagcttcat caaaatattc
     6601 tccacacata gaccataact ctgtaatcaa atattccata tcttcatggt cttctgtgat
     6661 ggcttctctt gataaataat ttttgatttt taaaaacctt cttccttcct cgtaatctac
     6721 ggtaattaac ctgatatctg agtgtatcat gcccagcatt gctatattca taccgactcg
     6781 cagccagctt ggtaatatat tcataactat tgcacctttt taatggtttt agggttagcc
     6841 ggaacaatgt gaactccatt tttacctgaa tgaatcgtac caaaattggt acttaatcct
     6901 gatttcccat cgctgcctat aggataccca aaatcaacaa caggatttcc tcttgctcct
     6961 ttagaaataa ctggataagc acctgaatgt attccgttca acaattcttg aggattaatg
     7021 ttttgattta aagtactctt accttcaatg tagtttctat gtcctgaaat atgtttccct
     7081 tgagctccat catgaatttt agtcccaata gattttgctt taaaatcaac attgcgggtt
     7141 tgaaacattt ctccatctac agactgagat aatcttgaag cagtgttatg cttgtaggag
     7201 tctgagaaat ccccactaac cgcagccttc cccggttttg ccgcctttgt caggtttttg
     7261 actttggcaa acggcaggac gttgaccagg gcttcgacgg tttcggcggc attggggttt
     7321 tcctgcatcc actggtcaa
//
LOCUS       DAWGRZ010000103         7302 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000103 DAWGRZ010000000
VERSION     DAWGRZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.103"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(146..2749)
                     /gene="pepN"
                     /locus_tag="WHT44_001441"
     CDS             complement(146..2749)
                     /gene="pepN"
                     /locus_tag="WHT44_001441"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ2533615.1"
                     /translation="MSKTVHYLKDYQTPAYHILKTDLHFDINEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIAGVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEFSDGRHWVKWEDPFSKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNIFELTVKQTVPPTPDMTDKQPMMIPVKVGLLNRNGEAVAFDYQGKRATEAVLLLT
                     EAEQTFLLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLATLSDGVELPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGA
                     ENIDPLRYHQAREALLDTLAVHFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVRTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2880..3674)
                     /locus_tag="WHT44_001442"
     CDS             complement(2880..3674)
                     /locus_tag="WHT44_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ2533616.1"
                     /translation="MTTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHTGRLKEIRNYCETDAANTYL
                     MYLRFCLVSGRLDADEYEMEIKRMRNYLSAQAGEKPHWEEFVRAWE"
     gene            complement(4421..5290)
                     /locus_tag="WHT44_001443"
     CDS             complement(4421..5290)
                     /locus_tag="WHT44_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ2533617.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIADLTGLLAYLLVKPRRRIGEIN
                     LAKCFSEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDIPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWKSFPGEDAKADAQRMNRFIEDRVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5341..5877)
                     /gene="ruvC"
                     /locus_tag="WHT44_001444"
     CDS             complement(5341..5877)
                     /gene="ruvC"
                     /locus_tag="WHT44_001444"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ2533618.1"
                     /translation="MAASVRILGIDPGSRVTGFGVIDVRGRDHFYVASGCIKTPADAP
                     LADRIAVIVRHIGEVVTVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQPDAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(5880..6119)
                     /locus_tag="WHT44_001445"
     CDS             complement(5880..6119)
                     /locus_tag="WHT44_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ2533619.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6149..7159)
                     /gene="dusB"
                     /locus_tag="WHT44_001446"
     CDS             complement(6149..7159)
                     /gene="dusB"
                     /locus_tag="WHT44_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ2533620.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFADEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVRAAGVPVTLKTRLGWHDDHQNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGDITSPQKAQAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECAAAILNHIRAIHAFYGDTAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAEKTDSWACAYRPN
                     AF"
BASE COUNT         1608 a         1931 c         1934 g         1829 t
ORIGIN      
        1 gtcgccttgt cctgattttt gttaatccac tataattaat atgactttgc ggccgttttg
       61 ccattgcgta ataaaacgat ggggaagtga tgataaaacg tgtgtgtaac tatatcagac
      121 ggcattgttt ttctgtttga cggcctcaat ccaaaatttt gccgacgatt tcgcccacgt
      181 ctttcgacaa tccttcctgc gcccgaatgc gctgcaatgc ttgtttcacc aagtttttgc
      241 ggtgcggctc gagcttgttg cagaggttga acgcctgcac taagcgggcg gcgacctgcg
      301 ggttgaagcg gtcgatttcg atgactttgt cggcgatgaa gcggtagccg ctgccgtctt
      361 ctgcgtggaa atgcgggacg ttgcggctga agctgccgat gagcgaacgg gctttgttgg
      421 ggttttcgag gctgaatttc ggatgctgca aggcggttcg aacctgttgc agggtgtcgc
      481 tgcggcggct tgagccgacg agggcaaaat atttgtccat caccagcgcg tcgtctgaaa
      541 acttgtcggc aaactgcgcc agcaggcggt tgcgcgtatc gctttcgttg ccgttgacgg
      601 cggacaggat gccccattcg tgggtcatgt tttgcgccat ttcgccgtat ttttcggcaa
      661 cggtttcgat gtgcgcgggg tcggcgcgca ggacaaaggc gcggcagacg ttgcgcagcg
      721 tgcgccagcc ggcggcttcg gggctgtatt cgtagctttg gttttcctgc ttcgccgcct
      781 gacggttcaa ttcgtgccat ttcggcagga agtggacggc aagcgtatcc aacaaggctt
      841 cgcgcgcctg atggtagcgc agcgggtcga tgttttctgc gccgtcccac agctcggctt
      901 cggatggcac gcccaaaagc agggctttga aggcgttgtc taagaggtcg tctgaaatga
      961 ctttttcgac ggcggcaagc agtttttcgt gtttcggcag ctcaacgccg tctgaaagcg
     1021 tggcaaggtt ggcggcgacg gcgcggcggt agagcgtttg ggcggcttcc cagcgcgtga
     1081 aggcgtcgct gtcatgggcg agcaggagca gcaggtcgtc gtcgctgtac ggatagttca
     1141 gatgcaccgg cgcgctgaac ccgcgcagca gcgagggaac gacggcttcg gttacgcctt
     1201 cgagcaggaa ggtctgttcg gcttcggtca gcagcaacac ggcttcggtc gcgcgtttgc
     1261 cctgatagtc gaatgccacc gcttcgccgt tgcggttcag cagcccgacc ttgacgggaa
     1321 tcatcatcgg ctgtttatcc gtcatatcgg gcgtgggcgg cacggtttgt ttgacggtca
     1381 actcgaaaat attgtttttc agacgacctt ccgcttccaa aacgggcgtg cccgcctggc
     1441 tgtaccacaa ggcgaactgg tcgagattga tgccgttcgc gtccgccatc gccgcgcgga
     1501 aatcgtcgca ggtaacggcc tgtccgtcgt ggcgttggaa atagagcttc atgcctttct
     1561 ggaagccctc ttcgccgagc agggtgtgat acatccgcac tacttccgcg cctttttcat
     1621 aaacggtcat ggtgtagaaa ttgttcatct cctcatagct ggcggggcgc accggatggg
     1681 cggtcgggcc tgcgtcttcg gggaactggt gctggcgcag caggcggatg ttttcgatgc
     1741 ggcgcacggc gcggctggcg cggtcgccgg aaaattcttg gtcgcggaac acggtcagcc
     1801 cttccttcag cgaaagctgg aaccagtcgc ggcaggttac gcggttgccc gtccagttgt
     1861 ggaaatactc gtgtccgacc acggattcga tgccttcgaa atcggtatcg gtggcggtgc
     1921 ggctgtcggc aaggacgaac ttggtgttaa agatgttcaa acccttgttt tccatcgcgc
     1981 ccatattgaa atcgcccacg gcgacgacca tgaaaatatc caagtcgtat tccaaaccga
     2041 agcgcgtttc gtcccatttc atcgcgtttt tcaacgattc cacggcaaag ccgaccttgg
     2101 gcttgtccgc ttcggtggtg taaaactcga ttttgacgtt tctgccgctc atggtggtga
     2161 aatagtcttc cgttaccgcc aaatcgcccg cgaccaaagc aaacagatag ctcggtttgg
     2221 aaaacgggtc ttcccatttc acccaatggc ggccgtctga aaactcgccg ccgtcgattt
     2281 tgttgccgtt ggaaagcaaa acgggatagc gttttttgtc ggcgacgatg gtggtggtga
     2341 acttggacat cacatccgga cggtcgatgt aaaatgtgat tttgcggaag ccctccggct
     2401 cgcactgggt aaacaaattg ccgccggaag catacagccc catcagcgat ttgttttccg
     2461 ccggcaggat ttcggtttcc acttcgacgg tgaagcgttc ggacggcacg cccgcaatcg
     2521 tcagcgtctc tccttccaac acataatccg ccgccgcccc gttgattttg acggacaaga
     2581 gtttcgccga accgtccaac accagcggct cccctaccct ctgcggctca accgtcaaac
     2641 gcgacttcac gacggtttgc ggttcattaa tatcaaaatg taaatcggtt ttgagaatat
     2701 ggtaggcggg cgtttgatag tctttgagat aatgcacggt tttgctcatt tttttctttc
     2761 aatgttattt tgtttgactg gaaaaggctt cagacggcac gggcgcatcc cgcgtatgcc
     2821 gtctgaagcc gcagcggcgg cacgggcgcg ccgccggaca accggtttga attcaatctt
     2881 tattcccacg cgcggacaaa ctcttcccaa tgcggctttt ccccggcttg tgcggacagg
     2941 taattccgca tccgtttgat ttccatttcg tattcgtccg catccagcct gccgctgacc
     3001 agacagaaac gcaggtacat cagataagtg tttgccgcgt cggtttcgca ataattgcgg
     3061 atttccttca gcctgcccgt atggaatgcc tcccaaacct tgctgccgtc catacccagc
     3121 ttgcccggaa aaccgcacag tttcgccata tcgtccagcg gcacgtttgc cctcggctgg
     3181 taaagcgcga gcaaatccat caaatcgcag tggcgttggt gataacggct gatgtagttg
     3241 ttccacttga aatcgcggct gtcgccgaaa tcgccgtcgc ccatatccca atagcgcgcg
     3301 gcgttgatgc cgtatatcag ggagcggtaa tgcagtacgg gcagatcgaa accgccgccg
     3361 ttccaactga ccagttgcgg cgtatgtttt tcaatcaatt cgaaaaattt agcaatgacc
     3421 acttcctcgc cgtcatccat ctcgccgatg gtgccgacat gtactttatc ctgcccccaa
     3481 cgcatgcagc acgaaatcgc cacaacctga tgaagatgat gctgcataaa atcgccgccc
     3541 gtctgagcac ggcgtttttg ctgggcaaac agcaccactt catcgtcggg cagcgaggac
     3601 ggcagctcgt acaatgttcg gataccctgc acatcgggta cggtttcaat atcgaaagcc
     3661 aaaatcgtgg tcatgacagc accttgtatt taaaacggat gcacctattg tgtcattaaa
     3721 aggcggataa aaaaagaggc aaccccccac aggattgccc caatacctca aatcagagat
     3781 ttacgcttca caaacaatac aggctttcgc ctgcggcttt acccgcgtag ctcaactcta
     3841 cgccggcaaa ctttcgtttc accgtttccg atgaaacccc gaccaatcgc aagactgacc
     3901 ggaaaatcct ttcagacggc atttcctgcc tgtcgtgtaa ttccatgtag cgaaatgtac
     3961 gccattttct acgctttgcc aagcattttt tacaatataa atgtcaaaac attaatttta
     4021 taaaattgct gaaaatatta aatatatgga tttttatttt ttatatttca ataaatataa
     4081 atttaatttt gatttatatt taaatttaag cataaaatgt caaatattaa agtaaacatg
     4141 aaaggcatat attaaatatt tatttataac gctatgtttt taaagaaaat taattttaat
     4201 atattaacta gattgtctgc atatattcat aggtttgcgg tatttcttcc aaaacctgct
     4261 tcgaatttcc cgaccaagtc ttaaaaatat tgtttttgag atacttaaat agcagcgatt
     4321 atcaaatgaa atctgttcat ataatctgcc attttgcatt taaaaaaaca atcaggagtt
     4381 tcgactcgaa acgcctgata tgttttgtaa ttttacgtag tcagtaaaaa tcggggctgc
     4441 cttccggacg ggttttaaaa cgcttgtgca gccaaaaata ttgttccgga tgttcgcgca
     4501 ccctgtcttc gataaaacgg ttcatgcgct gcgcgtcggc tttcgcgtct tcacccggaa
     4561 aagatttcca agcagggtag aaatgcaatg taaccgtatt gtctgcctcg cggacgggaa
     4621 tggcgggtat cacttttgca tttgcaagcg cggcaatgcg gctcaatccg gtaatcgttg
     4681 ccgtctgaat accgaaaaaa tccacaaaaa ccgaatcgtt gcgtccgaaa tcctgatcgg
     4741 gcagatacag aaacggcgcg ctgcttttgc ggaactgttt gacgagggcg cgcagccctt
     4801 cggtgcgccc gataaggaag acgttgtgat agcggttgcg gcctttcaaa atctgttcgt
     4861 ccaatatctt gtttttttga tgggaataca tactgatcag cgggatatcc tgattaagcg
     4921 cgtacaccgc catctcgaac gcggtgaagt gcggatacag gatgatgact ttttcccccg
     4981 ccgccagcgc gtcgtccaaa taatgcttat tgcggtagcg caccagcgat ttcaaacgtc
     5041 cggcaggcgc gtaccaatat aaaccgtatt ccaacatcag tttcgccatg tgtttgaaat
     5101 gctgtttcaa cacggtttta cgcttttcct cactccattc ggaaaaacat tttgccaaat
     5161 tgatttcgcc gatacggcgg cgcggtttga ccagaaggta ggcaagcaaa cccgtcaggt
     5221 cggcaatctt gtgcagcagc gcaaacggca gaaactgcaa aacatacagt acaaaaaata
     5281 taaatttcat ctcgatacac attttctttt cagacggcaa aatacaaatg ccgtctgaaa
     5341 ctattgaaac ctgccgcgct tgacctgcat ccccgaagga ttgagtttgg cggcaagccc
     5401 gtggttgcgt aaggcgtggg tcagcgcgac ggcaagaccg tccgccgcat ccggctgggg
     5461 cgttcccgaa agtcccaaca tctgcaccac catatgctgc acctgttctt ttgccgcctt
     5521 gcccttgccg actaccgcct gtttgacctg caaggccgtg tattccgaaa cgggcagctt
     5581 atggctgacc aatgccgcca atgccgcgcc cctagcctga ccgagcatca gcgtcgatgc
     5641 cggattgacg ttgacgaaca cctgttccac tgccgcctgt tgaggcttgt aaacggtaac
     5701 gacttcgccg atgtgccgga cgatgacggc aatcctgtct gccagaggcg catcggcagg
     5761 cgttttgatg cagccggagg cgacgtaaaa atgatcccgc cccctgacat cgatgacacc
     5821 gaaacccgtt acgcgactgc ccgggtcgat gcctaagata cggacgcttg cagccatatt
     5881 cacaacaaac cgtgttgaat cagcttctta cgcagggtat tgcggttcag ccccagcatc
     5941 acggatgctt tggactggtt gccgccgcat tgctccatca cgcacaccag cagcggtttt
     6001 tccacctgat gcaataccat atcgtacacg ccgcaaggtt cggtaccgtt caggtctttg
     6061 aaatattgtt ctaaattttg tctgatgcat tgggaaatat cgggaagggt atggggcatg
     6121 attgcacttt caaaggataa tcaagtgttc agaaggcatt tgggcggtag gcgcacgccc
     6181 aactgtcggt tttttcggca agtctttcaa gataacctgc aagcatgtcg tattgcgccg
     6241 ccgcactgtc caagcggttg atttcacgac gtgtctgttc gccgtcgggc atttcgtcga
     6301 tgtaccagcc tatgtgtttg cgtgcgatgc gcacaccggc ggtgtcgccg taaaacgcgt
     6361 gtatggcgcg gatgtggttc aaaatagcgg cggcgcattc tgccaaactc aaggcaggcg
     6421 gcaaaacacc gtgttcggca taatgtttca aatcgcggaa gaaccacggc ctgccttgcg
     6481 cgccgcgccc tatcataatg ccgtcggcgg cggtttgttt gaggacggct tgggcttttt
     6541 gcggcgaagt aatgtcgccg ttgacccaga ccgggatgtt cagacggcat ttggtttcgg
     6601 cgatgagttc gtaacgcgct tcgcctttgt acatttgcgt acgcgtgcgt ccgtggacgg
     6661 caagggcggc gatgccgcaa tcttcggcga ttttggcgat gacgggcagg ttttgatggt
     6721 cgtcgtgcca acccaaacgg gttttgaggg taacgggtac gcctgccgca cggacgacgg
     6781 cttccaaaat ggcggcaacc agcggctcgt tctgcatcag cgcgctaccg gcttggacat
     6841 tgcagacttt tttagcggga cagcccatgt tgatgtcgat aagctgcgcc ccaaggctga
     6901 cgttgtaacg cgcggcatcc gccatctgct gcggatcgct tccggcaatc tgcacggcaa
     6961 caatgccgcc ttcatcggca aaatcgctgc ggtgcaaggt ttttctagta tttctgagcg
     7021 tcgggtcgct ggtcagcatt tcgcacaccg cccaacctgc gccaaaatct cggcaaagtc
     7081 ggcggaacgg tttgtcggta atgcccgcca tcggcgcaag tgcgatgggg ttgtcgataa
     7141 aatagccgcc gatgtgcata atggatccgc gtttcaaaaa agtacgccat tgtacatttt
     7201 ttaagcagga tttccaatct ccggacgcgc ccgcgattgg gtcggacacc gttttatggc
     7261 ataatccgca cacagattcc ctgccccgcc actcacaggc gg
//
LOCUS       DAWGRZ010000104         7290 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000104 DAWGRZ010000000
VERSION     DAWGRZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.104"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(128..940)
                     /locus_tag="WHT44_001447"
     CDS             complement(128..940)
                     /locus_tag="WHT44_001447"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ2533621.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRNNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            1142..1603
                     /gene="tsaE"
                     /locus_tag="WHT44_001448"
     CDS             1142..1603
                     /gene="tsaE"
                     /locus_tag="WHT44_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ2533622.1"
                     /translation="MSDLPSISRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGDGRKCLLTAHTERGRESLPL"
     gene            1600..2850
                     /locus_tag="WHT44_001449"
     CDS             1600..2850
                     /locus_tag="WHT44_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ2533623.1"
                     /translation="MIKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPTHAQVFALPPVGGFKNRLVVDLYPHGMDADDPMMALLNGS
                     LNKTLRGSPEADLAQNTTPQPGRGRNGRRPVIMLDPGHGGEDPGAISPGGLQEKHVVL
                     SIARETKNQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(2908..4050)
                     /locus_tag="WHT44_001450"
     CDS             complement(2908..4050)
                     /locus_tag="WHT44_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ2533624.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGL
                     EQVYAANLHSRTASRILLRLTKGTYRNERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLNERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRA
                     PGMMRRFGFEKLQNFDKTLWSDLRRRAEAQTRPVRAPIAGSDNDRRIVQTALDNARRA
                     GVDDIVSFSVADAQSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WLAAMFTGDREMPKFMCLSPKRKIPLYNGNIDCRLFLIDMVEGSNR"
     gene            complement(4099..4521)
                     /locus_tag="WHT44_001451"
     CDS             complement(4099..4521)
                     /locus_tag="WHT44_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533625.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(4514..5323)
                     /locus_tag="WHT44_001452"
     CDS             complement(4514..5323)
                     /locus_tag="WHT44_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ2533626.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWTGKPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMVVQTREQWRRAQEKGADAVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(5405..5782)
                     /locus_tag="WHT44_001453"
     CDS             complement(5405..5782)
                     /locus_tag="WHT44_001453"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ2533627.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(5982..6749)
                     /locus_tag="WHT44_001454"
     CDS             complement(5982..6749)
                     /locus_tag="WHT44_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ2533628.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(6759..>7290)
                     /locus_tag="WHT44_001455"
     CDS             complement(6759..>7290)
                     /locus_tag="WHT44_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ2533629.1"
                     /translation="DITFSEDVGECPLHSDSSFSENPESYLVMYVVKSANDGGNSLFL
                     SSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPASFDKKQGVRWGNILSVNTQMIRFRS
                     DCIYKGIEENRNKVSKEMVLALDYLINVIKNASDIQEFSAQDDGLIIIDNVNGLHART
                     DYTDKNRHYIRARITV"
BASE COUNT         1746 a         2002 c         1800 g         1742 t
ORIGIN      
        1 agaacgtaaa atctaaagaa accgttttat ccgataagtt tccgcaccga caggtctaga
       61 ttcccacttt cgtgggaatg acgggatttt aggtttctgt aatctcgggc agctttccgt
      121 catcgtttta acccaaagac accatttcaa tctgctccat cgtcctgccc agaaaacgct
      181 cgccgatggt tctgaatttc aaaggaatat cgctgacgta aaaacggtag tcgggattgt
      241 tgttgtcggt attgagcaat ccttcctgag caaggacgcg tgcggtttct tcggccgttg
      301 taattgcaga atcaaccaac gcgacattgc ccgcctccct gccgattaag ggcttgagca
      361 agggaaagtg cgtgcagccc aacaccagcg tatcgatgcc gtctgcaagc aatggtttga
      421 ggtattcgca tacggtcagg cgggtaactt cgtgttccag ccagccctct tccaccaaag
      481 ggacgagcag cggcgcggcc tgcgtgcgga cgagcgtgtc ggggttgttc ctatggatgg
      541 cgcgcgcata agcattgctg ttgactgtcg tattggtggc gataatgccg attttattgt
      601 tgcgcgtcgt tgccagcgcg gctttcgcgc cggcggaaat cacgtccaaa acgggcatat
      661 tgccggtttt ttgacggatt ttctgccccg ccaccgccgc aatcgtattg cacgcgataa
      721 ccatcgcctt gacatcgtgt tccaataaaa aatcgacaat ctgcatcgag aaattttcga
      781 tggtcgcctt agatttcgtc ccgtaaggca cgcgcgccgt gtcgccgaaa taaatgatgt
      841 tctccatcgg cagccgttcc atcagcgctc gcacattggt caaaccgccg attcccgagt
      901 caaaaacgcc gatgggtcgc tgcctgccga tattttccat tctttttcca atccgtccta
      961 aacatacaat ccgcgtattg tacacgcgcc gctttttctt gacagccgtt gccgtctgaa
     1021 agcagaacgc ggatttggct gttataatgc tcggacaaga agcatccgcc cctcgggtgc
     1081 aaagtttgca aaacctgcaa aactgcccat aataccctag accttacaac aaaccgtttc
     1141 catgtctgat ttaccctcta tctcccgatt ccttgccgac gaagccgcca cactcgattt
     1201 gggcgcggcg tggtcttccc gtttaaacgc accgctggtc atttatctcg aaggggattt
     1261 gggtgcgggc aaaaccacgc tgacacgcgg catcctgcgc ggattgggtc atcagggcgc
     1321 agtcaaaagt ccgacctacg ccatcgtcga atcttatccg ctggaacgct tcaccctgca
     1381 ccatttcgac ctctaccgct tctcgttccc cgaagaatgg gaagacgcgg ggcttgacga
     1441 actgtttgcc gcaaacagcg tctgcctgat cgaatggccg caacagggcg gggaatttac
     1501 gccgcccgcc gacatcaccg caacattgac acacgacggc gacggcagaa aatgcctgct
     1561 gaccgcccat accgaacgag gacgcgaaag cctgccgcta tgatcaaact gacacgaaga
     1621 caaatcatcc gccgcaccgc cggcacactg ttcgccctaa gccccatcgc atccgccgtt
     1681 gccaaaacgg tacgcgcccc gcaattcacc gccgcacgga tatggccgtc gcacacctac
     1741 acccgcctga cgctggaaag caccgccgcg ctcaaatacc agcacttcac gctcgacaac
     1801 ccgggcaggc tggtcgtcga catacaaaac gcgaacatca ataccgtatt gcacggactg
     1861 tctcagaaag tcatggcaga cgaccccttt atccgcagca tacgcgcggg tcagaacacg
     1921 ccgaccaccg tccgcctcgt catcgatttg aaacagccca cccacgcaca agtcttcgcg
     1981 cttccgcccg tcggcggctt taagaaccgc ctcgtcgtcg acctctatcc gcacgggatg
     2041 gatgccgacg atccgatgat ggcactgctc aacggtagcc tgaataaaac cctgcgcggc
     2101 tctcccgaag ccgacctcgc ccaaaacacc acgccccaac ccgggcgcgg cagaaacggg
     2161 cgcagacccg tcatcatgct cgatccggga cacggcggtg aagaccccgg cgccatcagc
     2221 ccgggcggtc tacaggaaaa acatgtcgtc ctatccattg ccagggaaac caaaaatcag
     2281 ttggaagcat taggttacaa tgtatttatg acgcgcaacg aagacgtgtt catcccattg
     2341 ggcgtgcgtg tcgccaaagg gcgagcacgg cgggcggacg tatttgtctc catccacgcc
     2401 gatgccttca ccagcccctc cgcgcgcggc acgggggttt atatgttaaa caccaaaggc
     2461 gcaaccagct ctgccgccaa attcttggaa cagacgcaaa acaatgccga cgcggtcggc
     2521 ggcgtaccga ccagcggcaa ccgcaatgtc gataccgccc tgctcgacat gacccaaacc
     2581 gccacgctgc gcgacagccg caaactcggc aaactggtgc ttgaagaatt gggcaggctc
     2641 aaccatctgc acaaaggcag ggtggacgaa gccaatttcg ccgttttgcg cgcacccgat
     2701 atgccgtcta tcctggtcga aaccgccttc ctgtccaatc ctgccgaaga gaagctgctg
     2761 ggcagcgaat ccttccgccg gcagtgcgcc caatccattg cctcgggtgt ccaacgctac
     2821 atcaatacat ccgtattgaa gcggggttga ttccgaaaaa taaatgccgt ctgaacattt
     2881 ttcagacggc atttttgtac actttcctca acggttcgat ccttccacca tatcaatcag
     2941 gaacaggcgg cagtcgatgt tgccgttata aagcgggatt ttccgcttgg gcgacaggca
     3001 catgaatttg ggcatttccc tatcgccggt aaacattgcc gccaaccagc ctgcgtaatg
     3061 ttttttcaac cacgtcccca actgcggata cagtgcctgc aaggcgcgga cttcctcaag
     3121 gcgcacgccg tagggcggat tggacaccat aatgccgttt tcgccgttcg gtcggacgga
     3181 ctgcgcgtcg gcaacgctga aggaaacgat gtcgtccacc ccggcgcggc gtgcgttgtc
     3241 caatgccgtc tgaacgatgc ggcggtcgtt gtcgctgcct gcaatcgggg cgcggacggg
     3301 gcgggtttgc gcttcggcgc ggcgccgcaa atccgaccac agcgttttat cgaaattttg
     3361 cagtttttca aaaccgaaac ggcgcatcat acccggcgcg cggcgggcgg caatccaagc
     3421 ggcttcgata gcaatcgtgc cgctgccgca aaacgggtct tgaaacggct gcgtgccgtc
     3481 gtagcctgcc gagagcagca gtccggcggc aaggttttcg cgcagcgggg cttcgccggt
     3541 atccaggcgg tagccgcgtt tgaacagggc ttcgcccgaa gtgtcaataa agatttcgac
     3601 attgcgttcg ttcaaaaagg cgtggatgcg gacatcgggc gcggctttgt ccacgctcgg
     3661 acgtgcgtcg taaatgtcgc ggaaagcgtc gcagacggca tctttgacgg tcagtccgac
     3721 aaattggatg ctcttaacgt tggcacgctt tgcctcgact ttgactttga acgtctgctg
     3781 taaagtaaac caattaaacc agttgatatt tttggcgagt ttgtagatgt cgcgctcatt
     3841 gcggtatgtc cctttggtca ggcgcagcag gatacggctg gcagtacgcg aatgcaggtt
     3901 ggcggcgtaa acctgttcca atccgccccg gcaggaaacg ccgccgtcaa acacttgtac
     3961 atcggtacag ccgaggcttt cgagttcttg agataaaacg gtctccaagc cgcgcgggca
     4021 ggtggcgaaa agtgtatata aagtattcat aacgtgtcct ttccggacgg atgttccggc
     4081 aatggcggat tgaaaggttt attcggcggt aaatgccgtc tgaaatgtcc gactgccgat
     4141 tgtaatgtcc gccgtaaaat cgcgcctgcc ttcgacacag atgggcaggc ggatgcgtac
     4201 tgcctgccaa gtccccgacg gttgccgctc gaacatatag cggttgaaac ccatatccat
     4261 atttttcata ctgaagctga tgctgacctg ttccgtgccg gcgggcgcgt gttcgatata
     4321 aatatcaaac ggttttttgg ttgaaacggc ggcggcgcgg acgcggcttc cgtccggcag
     4381 cgtgcaaccc tcggtcaaat cgcattgcgc cgccacagct tgcggctgct gcgcctgcca
     4441 ccattgcaac aaaacgagct tgacggcagc aaaggcaatc agcagcagtg cggcaagcag
     4501 taatttacgg tttttattca tgattcttcc ctattgtttc atcccttacc accacgtgcg
     4561 cccccaatcc gagccatagt tttccataac gtgcaaccgt ctgtccgttt gctgcaagta
     4621 cgagcggcac ggatacgccc tgccgcaggg cttctgccgc ctcttgtgcc tgaacatcat
     4681 cgcacacgcg ccaaacgacc gcatccgcgc ccttttcctg cgcccgccgc cattgttcgc
     4741 gggtctgcac caccatccac acgctgtcgg cgtgttcggg tctgtcctgc cattgcgccg
     4801 cctccatcaa aacgttcgca ccgcttccct ctgccgaacc caaaggcagg acgcggtacg
     4861 cgcccatact gttttctccg tgcaaacccg ttttcaggct gccgtacaaa cggcgcggga
     4921 cggacagcga acgcaaaagc gcgccgttgg cgggcagcat gggggcaacg gtaaaatcac
     4981 ccgccttctg ccaagaccat tcctgccctt cgcgggattg cggtttgccc gtccattggt
     5041 cggggttgac ccataggaat ttcaggcaga cgcgggcgtg ttcgtaggaa tggattttgg
     5101 tcaaccaagg cgtggcggcg aggatgcgga tgccgagttc ttcttcaaac tcgcgttgca
     5161 gggcttggaa gtcggtttcg cccgcttcga ccttgccgcc ggcaaattcc caatatccgg
     5221 catagggttt gccttcgggg cgcgagctga gcaggtagtt gccgtctgaa tcgagcagga
     5281 tgccggcaac gacgcggata aggggtcggg tgtcttgaat catggcgggt acgcggttgg
     5341 tcggtcggag tgcaaatgag ggacgggcaa tgccgtcaac gggtttgccg catttcagac
     5401 ggcattattt ttcggcaacg acaaacgcca atacggtgtc ttcttcgtcg ctcatgctga
     5461 ggctgacgcg gctgatgcct tgttcctcca gccatttgga cagggcgggg ccgtagaaaa
     5521 attcgggctt gcccaatgcg tcatgcccga tgccgatgtt gcggaaggaa accgcgccgc
     5581 gtatgcccgt gccgacggct ttggcaaagg cttctttggc ggcaaagcgt ttggcgaggt
     5641 agttgacggg tttgcccgct tgcggaaatt caagcagctc ttccggagtg aggatgcgcc
     5701 cggcaaacgc ctgtccgaat tttttgttta agcggatgat gcgcttgagg gaaacaatgt
     5761 ctgtgccgat gccgtaaatc atatttgcgc tccttcgccc ttggttgcgg gtagtgatga
     5821 atgtgatgat gaatgccgtc aatttcttgg ggcgttaaaa tggcgtgaat atccactttt
     5881 ggggttttgt cggtaacaat tttcactgta accttttgca tttgaaattc taaaacatcg
     5941 tgccaattat ttaataagga gttaaggcat ttaataatga attaaagttt gattaaaccc
     6001 gaatgcccga ttttcagagc caataccaat aaaacaataa aaattaacat tttaaaaaaa
     6061 tttgggctaa tcttagtcct taaccgaatt ccaacataca atccaataac agacaatacg
     6121 gacagtaaaa atattaaacc gtattcactc ttatttaata accaatactg gtctcttagc
     6181 atatatattt gaacaatttt cgccaaaaga tagcatagat tgcttgattt tacgatacga
     6241 tttttatttt ctgtttcgct aagcaaaaat attaacaata tgggagacat ggcattggtt
     6301 gaaccgccga tgatgcctgc caaaaaccca aaaagaacca tattcttatt attggcaact
     6361 acttgaatat tttttgcttt tgcacataca tttaaaatac cattgacaga ataatacaat
     6421 gtaatgattg ccatcagtaa aagcagccaa gacactggaa gtatcaaaag caacttcacc
     6481 cccaaaatgc tgccaacgac gctgccgata gcaagcaatt tataggtttt taaataataa
     6541 acaatctctt gccaaaaacc ctttttgtta ttgctgcata gaaccaacaa gctcattaac
     6601 aggcttggta atgccaccaa ggcaacaacc ttagacaatg gcatgataaa agccaatgcg
     6661 gttgtaccga gcatcggaaa tcccatgcct gtaattccgt gcagtattgc ggcagcaaca
     6721 aaaacgatag attgcattat ttcttgcata accgtccttt atacagtaat tcttgctcta
     6781 atataatgcc tgtttttatc cgtataatca gttctggcat gcaagccatt gacattgtca
     6841 ataataatca aaccatcatc ttgtgcagaa aattcttgaa tatcactcgc attttttata
     6901 acatttataa gataatcaag tgcaagtacc atttcctttg atactttatt tctattttct
     6961 tcaatacctt tatagataca atcacttcta aatctaatca tttgagtatt gaccgataag
     7021 atattacccc atctcacacc ttgtttttta tcaaatgatg ctggtgtttt aaatggatat
     7081 aaattgcccg ttaatgtttt taagtgtttt ttaccggttt ctgttttaga taactgattg
     7141 acaatatctg atgaacttaa aaatagggaa ttacctccat cattggctga ttttactaca
     7201 tacataacca aataactttc cgggttttca ctaaaagatg aatcactatg aagtggacat
     7261 tctccaacat cttccgagaa agttatatca
//
LOCUS       DAWGRZ010000105         7236 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000105 DAWGRZ010000000
VERSION     DAWGRZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7236
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.105"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(76..831)
                     /locus_tag="WHT44_001456"
     CDS             complement(76..831)
                     /locus_tag="WHT44_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ2533630.1"
                     /translation="MKYVRLFFLGAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(956..2128)
                     /gene="nirK"
                     /locus_tag="WHT44_001457"
     CDS             complement(956..2128)
                     /gene="nirK"
                     /locus_tag="WHT44_001457"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HEZ2533631.1"
                     /translation="MKRQALAAMIASLFALAACGGEPAAQAPAETPAAAAEAASSAAQ
                     TAAETPSGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMTMEDGVEYRYWT
                     FDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTST
                     FSFKALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKG
                     KKGAQGLQPFDMDKAVAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLV
                     SSFHVIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRA
                     FNKGALGQLKVEGAENPEIMTQKLSDTAYAGNGAAPAASAPAASAPAASASEKSVY"
     gene            2499..4754
                     /locus_tag="WHT44_001458"
     CDS             2499..4754
                     /locus_tag="WHT44_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ2533632.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQTTGGMEVGSILGHGAYQAPDWTADWLHRELVAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRVKEDGSVVISDTRVKAIESILPYYHGVY
                     SDDPKFQTTREHFAMKNNTLPSQEAREKLFNFFFWTSWSASTNRPDENFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGAIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLVFIGGAICVAI
                     QATKIVFGRDK"
     gene            4933..5466
                     /locus_tag="WHT44_001459"
     CDS             4933..5466
                     /locus_tag="WHT44_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ2533633.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5529..6713)
                     /locus_tag="WHT44_001460"
     CDS             complement(5529..6713)
                     /locus_tag="WHT44_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ2533634.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNVGFTVTNQQAGSRRF
                     QRFAGTDTQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(6753..7157)
                     /locus_tag="WHT44_001461"
     CDS             complement(6753..7157)
                     /locus_tag="WHT44_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ2533635.1"
                     /translation="MTAQPCPICTAQNEDVLLQTPNLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         1619 a         1770 c         2024 g         1823 t
ORIGIN      
        1 ggattaacaa aaaccagtac ggcaaggcga ggcaacgccg tactggtttt tgttaatcca
       61 ctataattga aggggttatc ggcttgtgca acggaagccc aagttgtgca agacatattt
      121 ggattgcagg ctggtacgga tgccgtagcg gaggaaggcg gcatagttgg acgagtcgct
      181 cgacccgata gacgcgccgc tgcaaaacat ttgcgcgttg gcattgccgg aagaaagcag
      241 gctgctgttg aaatcttccg tccattccca aatcagcccg tgcatatcat aaacgcccca
      301 gtagttcggg cggcctttgc cgacatcgtg caggcctttc cgtccgccgt cggcatacca
      361 atcgagaata gtgcggttgt agccgggttc gtttgagccg tttttctgcg tggcggaagc
      421 aagtccggca aattcccatt cgtcaatggt cggcaggcgt ttgccttgtg cggcgcaata
      481 ggcgttggcg gcaaaccagg aaacattggt taccggttgt tttaattcgc ccgccttcgg
      541 cgcatagctg cggctgccgt ttttcatcca atgcttcagg taagcgggtt ctgcctgttt
      601 ggaaccgatc ctgccttttt gccattgggg gtggctgttg acaaattcgg caaactcggc
      661 attggtaacg ggatatttat ccagtttgaa cggtttgact ttaatcaggc cggtatcttt
      721 tttcagataa agcgggcggt agctgccgcc ttcgatttga accatttcgg cagccgccgc
      781 ttgagtgccg gcgagtgccg cgccgaggaa aaataaccgg acatacttca taaagcctcc
      841 tgacaggcgg ttaaaatcaa tcttccgaaa ggaaagattg gttgttaaaa aaccaccgcc
      901 gtgcgtaatg aagtacagcg gcagtggttc gtcccgctaa tgacgggtat ccaatttaat
      961 aaacgctttt ttcggatgca gaggctgccg gggcagaagc tgcgggagcg gaagcagcag
     1021 gagctgcacc gttaccggcg taagcggtat cactcaattt ttgagtcatg atttcagggt
     1081 tttctgcacc ttctactttc aattgaccca gtgcgccttt gttgaatgcg cggaagatag
     1141 agtggtcaac caaagtgtag ctgcccggga tgtcgacttt gaattcgacg atggcagagc
     1201 cgccggcagg aacgatggtg ctttgtacgt tttcgttaat cagtttgccg ccttcaacat
     1261 aaactttgtc gaagatttcg ccgatgacgt ggaaggaaga taccaagttc ggaccgccgt
     1321 taccaacgta catacgtaca gtttcgcctg ctttggcttt cagcgcgtta tcgccggcga
     1381 tagcacctac gtgaccgttg aatacgacgt attcaggctg ttcggcaacg gctttgtcca
     1441 tatcgaacgg ttgcagacct tgcgcgcctt ttttgccttt ggtgtagaag tcgccttgga
     1501 cgatgtagaa ctctttatcc actttcggca ggccttcttt aggctcgacc aaaatcagac
     1561 cgtacatacc gttggcgatg tgcataccga ccggtgcgac ggcgcagtgg tagatgtaca
     1621 gacccggttg cagggctttg aagctgaatg tggaagtacg gcccggagcg gtaaaggttg
     1681 cggccgcgcc gccgccctgg ccggtagccg cgtggaagtc gacgttgtgc ggaacggtag
     1741 aagaaggatt gttggaaaat tccacttcaa ccgtatcgcc ttcgcgtacg cggatcatac
     1801 ggcccggaac gtcgccgtca aatgtccagt agcggtattc cacaccgtct tccatggtca
     1861 tggttttttc gacggtttcc atttttacgc ggactttggc ggggtagtcg cggtcgattg
     1921 caggaggcac ttcgggagcg tgggtggtaa ccgcatcgat aacgggcagt tcgccggacg
     1981 gtgtttcggc ggcggtttgt gcggcggagc ttgcggcttc ggcggcagcg gcaggggttt
     2041 cggcaggggc ttgcgcggca ggttcgccgc cgcaggcggc taatgcgaat aaggaagcaa
     2101 tcattgcagc taaggcttgg cgtttcataa tattttcctt ttgtaagaaa agtagaggga
     2161 ttggttaaga acttggtgtg attatgcgtt tgccccattg ggggcatttt gatttaagtc
     2221 aaataattca taaaatatga acttaaaatg atacttctaa tactttatag ctactaaaaa
     2281 ttacaataaa taggctgatt tgcttaaggg cttcggcaaa tcaggcaaag gcagggtagt
     2341 tgccgactaa agaataatga tagttattat catatatgta tttgttttat atgtgagttt
     2401 ctttgaacaa atgtttattt gcgcggtaaa ccgtgctaca atctttaaca ttcatatttt
     2461 gtgaatttta atccactata ttaaaaggag ctctcaaaat gggacagtac aagaagcttt
     2521 ggtacttgct gtttgccgtt ctggcggtat gctttaccat tcttggctat atgggcagcg
     2581 aggtttacaa aaaagccccg ccttaccccg aacaggtcgt ttccgcatcc ggcaaggtct
     2641 tgatgacgaa agacgatatt ttggcaggtc agtctgcatg gcagactacc ggcggtatgg
     2701 aagtcggttc gattttgggt cacggtgcat atcaggctcc ggactggacg gcggactggc
     2761 tgcatcgtga gttggttgcc tggttggatt tgaccgcgca acagacttac ggcaaaaaat
     2821 tcgacgaggt ttcccccgag gaacaagccg tcttgaaaac acgcctggcc gacgaatacc
     2881 gcaatcaaag ccgcgtgaag gaagacggca gcgtcgtcat cagcgatacg cgcgtgaaag
     2941 ccatcgaaag catcctgcct tactaccacg gtgtgtacag cgacgatcct aagttccaaa
     3001 ccactcgcga acactttgca atgaaaaaca atacattgcc aagccaagaa gcgcgtgaaa
     3061 aactgttcaa cttcttcttc tggacttctt ggtctgcttc gaccaaccgt cctgacgaga
     3121 attttactta caccaacaac tggccgcacg aacctttgat caacaacgta ccgaccactg
     3181 aaaactatat gtggtcattc accagcgttg ttcttctgct gatgggcatc ggtttgctga
     3241 tgtggggtta ttccttcctg accaaacacg aggaagtgga agtgccgtct gaagacccga
     3301 tttccaaaat ccaactgacc ccttcgcaaa aagcattggg caaatacgtc ttcctgacgg
     3361 tcgccctgtt tgtggtacaa gtattgctgg gcggactgac cgcgcactac accgtcgaag
     3421 gccagggttt ctacggcatt gacgaggcgt tgggctttga aatgtccgac tggttcccct
     3481 acgccctgac ccgtacttgg cacatccaat ccgccatctt ctggattgca accggctttt
     3541 tgacggcagg tttgttcctc gcgccgattg tcaacggcgg taaagatccc aagttccaac
     3601 gtgccggcgt aaacttcctc tatatcgccc tcttcatcgt ggtcggcggt tcttacgcgg
     3661 gcaacttctt cgcgttgacg cacatccttc ccccggaatt taacttctgg ttcggacacc
     3721 aaggttacga atacctcgat ttgggacgtt tctggcaact ccttttgatg gtcggtctgc
     3781 tgttatggct gttcctgatg ttgcgttgca cggtttccgc ctttaaagaa aaaggcgtgg
     3841 acaaaaacct gttggctatc ttcgtagctt ctatggtcgg tgtgggcgta ttctacgcac
     3901 caggcctgtt ctatggcgaa aaatccccga ttgccgttat ggaatactgg cgttggtggg
     3961 tggttcacct gtgggtagaa ggcttcttcg aagtatttgc cactgccgcc tttgcctttg
     4021 tcttctacaa tatgggcttt gtccgccgca gtaccgccac tgcctccact ttggccgctg
     4081 ccgccatctt tatgttgggc ggcgttccgg gcacgctgca ccacctgtac ttctccggct
     4141 ctacctccgc ctctatggca atcggtgcct gcttctccgc tttggaagtc gtgccgctgg
     4201 tgttgctggg ccgtgaagca tacgaacact ggtcttacca acacctgtcc gactgggcga
     4261 aacgcctgcg ttggccgctg atgtgcttcg tggcagtcgc cttttggaat atgatcggtg
     4321 ccggcgtatt cggcttcctg atcaatccgc cgatttccct gttctatatc caaggtttga
     4381 acacttctgc cgttcacgcg cacgccgcct tgtttggtgt gtacggtttc ttggcattgg
     4441 gcttcgtatt gctggttgcc cgttatttga aaccgaacgc gcagtttgac gacaaactga
     4501 tgacttgggg cttctggctg ctcaacggcg gcttggtcgg tatgattgcc atcagcctgc
     4561 tgcctgtcgg cgcgattcag gcatacgcct ccatcacgca cggtctgtgg tatgcccgca
     4621 gcgaagagtt cctgcaaatg gaaatcctcg ataccttgcg ttgggtacgt acggcagctg
     4681 acttggtctt tatcggcggt gcaatctgtg tcgccatcca agccactaaa atcgtattcg
     4741 gccgcgacaa ataagttttg cggtatgaaa tgagcaactg cacccgtttt cgggtgcagt
     4801 tttttattgc ggattgggac gggaatggaa aaggatgtgg attttttatt ttaatcggct
     4861 atacaattgc tgctttcatg ccgtctgaag cacgtttcag atggcatcgg tttacatcag
     4921 ataaggataa aaatgggtat ttacgatttt caaatgaaag atgcagaagg caatgcggtt
     4981 gatttgtcag gctatcgcgg caaggttctg ctgattgtca acacggcaac gcgttgcggt
     5041 ctgaccccgc aatacgaggc tttgcagaag ctgtatgcac aatataccgc agaaggcttg
     5101 gagattttgg attttccgtg caaccagttc cgcgaacagg ctcccgaaag cagcggagaa
     5161 attgcccaag tgtgtatgat gaagtttggc acgaagttta agattttcga caaaatcgaa
     5221 gtcaatggag caaataccgc gcccttgtat gcctacctga aatccgtcaa accgcaagat
     5281 aaaggcaatc atctgtttaa agatttcgtg ttgaagctcg cagctttggg agagaagcgc
     5341 gacgaaggcg acatcaaatg gaactttacc aaattcctcg tcaaccgcga cggcgaagtg
     5401 gtcgagcgtt ttgcgcctag tgttacgccc gaagaaatcg aagccgatat tcgggcattg
     5461 ctgtaatctg ccgccgtctt gggaaatgcc gtctgaaggc ttcagacggc atttgtccgt
     5521 ttgcgggttt attccgaaat atcggcaaca ccgctttcgg ggaagatggg caaatccggc
     5581 ggtgggagca gggtatccaa agtcagggcg gacgcatcga gtgtggggta gccttcaaac
     5641 gcgaccgaat aaccgctgtt tccgttcata cggattcggg cgtgcgcgcc tagagggaaa
     5701 gtgattttgt cggcaatgtg tccgaacggg aagcccgtca gcacggggat tttcgccgtg
     5761 cgcgaaacat ggttggcaac ggcagaaaaa tcataagacg gatcatagac atctcgaatt
     5821 ttttccatac ggaaattgcc gaacacgatg gcgcgctgtt tcttcaaaat acccgaaaga
     5881 tacagcgtat tgagcatacg ttcgatgcgg tagggctgtt cgccgacatc ttcgaggaac
     5941 aaaatgccgc cgtcgatgtc gggcatataa ggcgtgccgg cgagcgaggc gaggacgctt
     6001 aagttgccgc cccacaatat gccttcggtt tcgacatcgg cgcgttggat ataaggaaca
     6061 tcaacggtca ggcggttttg ggttgcaccc ttgataaagg catccatcgt aaacgcaccg
     6121 ggggcgggtt tgccaaaatc gctataagcc atcgggccgg caaaactcat catattgcct
     6181 tttgccaaca atgccagctg gacggcgcat acgtcgctga atccgaaaaa gagcgtgccg
     6241 tgttcgcgca tccttgcgcc gagcgaagca aaatcgatat gcggcagaat ccgcgccgca
     6301 ccgtaaccgc cgcgcaaacc catcagcact ttaggcgtgg cgacgcggcc ggaagcgacc
     6361 tcttggaaat cggcggcacg ttgcgtgtcc gtgccggcaa accgttggaa acggcggctg
     6421 cccgcctgtt ggttggttac ggtaaaaccg acattgtaaa ggcgggttaa ggctgtgttg
     6481 acgcggttgg tgtcttcggc aaatcctgac gaagcgacaa cgcgcagaag gttgccgtcc
     6541 gaactttgac ggcgtggcgt ttggagaggc acggttcggg ctttcacaac ggaatgggaa
     6601 gagggaaggg gcggaacgga tgtggcggat gtgccgcaag cctgaagcag ccccgcgccg
     6661 gcagcggcgg tgcaggtttt cagaaaacgg cggcgggagg tcggttcggt cataatttcc
     6721 ctttcatacg gtttcagacg gcatcggcgg gcttaaagca gctttttaag ctgttccgtc
     6781 caatcttgcg gcagggtcat accgtgtttc cggacggggt ttgcccaaat cggcgcgggg
     6841 aaagacgcat cgttttcaaa gcgggcgata atatgccaat gcaggtgcgg cacgacattg
     6901 cccaagctgg cgaggttgat ttttgccggc cggaacactt ggcgcatagc ggcttcgact
     6961 ttgtacacca tttccatcaa ttcgccgcgt tccgctgccg aaaggtcggt catttcggca
     7021 atatgcttac gccaaatgac gcggcagaat gcaggcgaac cgctgtcgtt atggacggcg
     7081 atgacgcgga ggttgggggt ttgcagcaaa acgtcttcat tttgcgccgt gcagatgggg
     7141 cagggttggg cggtcatttt tccttattcc tgaaagtgtt taaaaaacgg tagggtaatt
     7201 tctgatatag cggattaaca aaaatcagga caaggc
//
LOCUS       DAWGRZ010000106         7204 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000106 DAWGRZ010000000
VERSION     DAWGRZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.106"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(64..148)
                     /locus_tag="WHT44_001462"
     tRNA            complement(64..148)
                     /locus_tag="WHT44_001462"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Leu,seq:caa)
     gene            425..1078
                     /locus_tag="WHT44_001463"
     CDS             425..1078
                     /locus_tag="WHT44_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ2533636.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQVASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFNLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            1157..1810
                     /locus_tag="WHT44_001464"
     CDS             1157..1810
                     /locus_tag="WHT44_001464"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ2533637.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            1914..2813
                     /locus_tag="WHT44_001465"
     CDS             1914..2813
                     /locus_tag="WHT44_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ2533638.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            2810..4099
                     /gene="hisD"
                     /locus_tag="WHT44_001466"
     CDS             2810..4099
                     /gene="hisD"
                     /locus_tag="WHT44_001466"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ2533639.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            4145..5242
                     /locus_tag="WHT44_001467"
     CDS             4145..5242
                     /locus_tag="WHT44_001467"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ2533640.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGRIPNLIVLRTL
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALRHYGIISANIDSLK
                     NERERMFAELGKICRLNTFSSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            5239..6156
                     /gene="hisB"
                     /locus_tag="WHT44_001468"
     CDS             5239..6156
                     /gene="hisB"
                     /locus_tag="WHT44_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ2533641.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            6251..7120
                     /locus_tag="WHT44_001469"
     CDS             6251..7120
                     /locus_tag="WHT44_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ2533642.1"
                     /translation="MSANEYAQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
BASE COUNT         1882 a         2119 c         1722 g         1481 t
ORIGIN      
        1 ttagttttat ctatttcatt aaacagcaat agacaaaaaa aataaccgct ctaaaagcgg
       61 ttgtggtgcc cagggtcgga ctcgaaccga cacaccttgc ggcgggggat tttgagtccc
      121 ctgcgtctac caatttcgcc acctgggctg gtgaagaagt cgtcattata atggcttttg
      181 aaattctgta aacctttttt ttgaaattat tttatctgtt tttattttat ttttgatttt
      241 aaatagaatt tttattattt taatcttact gttctttccg ctccaaagat tctgtatgat
      301 tcggcaattc ctgccgtgca gacaacgtaa aaaaatacta cattaaatct gccaaacgcg
      361 ttaagatgga aatattcaaa ttccgtacga atcaggtttt gctatttatt cttgggagat
      421 tgtcatgttt tccgtaccgc gttccttttt gccgggcgtt ttcgtacttg ccgcgcttgc
      481 cgcctgcaaa cctcaagaca acagtgcggc gcaagtcgct tcttcaagtg catccgcgtc
      541 ggctgcggaa aatgcggcaa agccgcaaac gcgcggtacg gatatgcgta aggaagacat
      601 cggcggcgat ttcacgctga ccgacggcga aggcaagcct ttcaacctga gcgatttgaa
      661 aggcaaggtc gtgattctgt ctttcggctt tacgcactgt cccgatgtct gcccgacaga
      721 gcttttgacg tacagcgaca cgttgaagca gttgggcggg caggctaagg acgtgaaagt
      781 ggtgttcgtc agcatcgatc cggaacgcga cacgcctgaa atcatcggca agtatgccaa
      841 acagttcaat ccggacttta tcggtctgac ggcaacgggc ggccaaaacc tgccggtcat
      901 caagcagcaa taccgcgtgg tttctgccaa agtcaatcaa aaagacgaca gcgaaaacta
      961 tttggtcgac cactcttccg gtgcgtatct catcgacaaa aacggtgagg ttgccatttt
     1021 ctcgccttac ggaagcgagc cggaaacgat tgctgccgat gtaaggaccc tgctctgata
     1081 aaaccgtatg ccgtctgcac cgtcggcgcc tattcagacg gcattattgt ttcaaccgac
     1141 aaaggacatc cacaccatgc aggataatgc tttgaccatc gccttatcca aggggcgcat
     1201 ttttgaggag acgctgccgc tgcttgccgc tgccggcatt gttccgactg aagagcctga
     1261 aaaatcgcgc aagctgatta tcgggacgaa ccatgaaaac atccgccttg tcattgtccg
     1321 cgcaaccgat gtgccgactt atgtccgcta cggcgcggcg gacttcggca ttgcgggcaa
     1381 agacgtgctg atcgaacacg gcggcacggg gctttaccgg cctttggatt tggagattgc
     1441 caagtgccgc atgatggttg ctgtgcgtaa agggtttgat tacgaagcag cttcgcaacc
     1501 cggatgccgt ctgaagattg ccacaaagta tcctgaaatc gcggcatctc attttgccgg
     1561 caagggtgtc catgtggaca ttatcaaact gtacggctcg atggaacttg cgccgctggt
     1621 cggcttgagc gatgcgattg tggacttggt ttcgacgggc aacaccttga aggcaaacgg
     1681 cttggaagca gtcgaacaca tcgtcgacat ttccagccgc ctggtggtca acaaggctgc
     1741 tttgaaaacg aaatacgcgc tgctggagcc gattattcag gcgttcggcg gcgcagtgaa
     1801 ggcgaagtaa gcatccattt gaataaagat gcgttttcag acgaccctat ccgttcccgc
     1861 cgacaggtcg tctgaaaata tcaccggcag taaactgtat aggagaagtt aaaatggttg
     1921 caaaaataaa aaaattctca gattcaaccc tttccgtttt gaataacggc gagcgtcggt
     1981 tttatgtcta ttgtctgacc gacctgaaaa aagacaaaat cctctacatc ggcaaaggct
     2041 gcggtaatcg tatcttcgag catgaatggg ttgctagtcg ttcacaagat ccagtctccg
     2101 gcgagattat cgatcggaaa ctcaaagcca tctccaaatg caagaaactc ggtcgctata
     2161 tcatcagcta tcatctgact gaagtcgaag cactcgccgc cgaatctgcc ttaattcatt
     2221 ttgttaaatc tgtcttgggt aaaaaactca aaaataaaat tgccgggcat ggtccgggtg
     2281 gtattagcgt agaagaacta gatcgccgct ttggattctc ttctctccca cttaacgaga
     2341 ttaaccccga cgggctgatt ctcgccatca aaatccacaa tgctttcgat ttagatactg
     2401 acgaagaatt agactacctt ttcgacaacc aagacgatgc caacctcaaa tcgcgtacgt
     2461 tgggcaactg ggttatcggt aaagatgttg cttcaaaagt gaaatacgtt atcggcgttc
     2521 acaccggtct gcaaaacgct gttgtcagtg catacgaagt ggacggtttt gaaacaatgg
     2581 ttgaggaaac caaaaacggt agaaaacaat cccgttaccg tttccgcact acctctcgta
     2641 gcgaagaggt attagccaaa ctcggtctgc aacaaaaatg cctgcccgaa ttgaagtttg
     2701 gtagcggggg agaaaaagcg tatatcagac ccaaaacaga gacagaaact gaacaagaga
     2761 atattcagac gacccccaat ccaaaaataa aaaaggaaaa aaccaaatca tgaaaaaact
     2821 caacacccaa tcgcccgatt tccaagccgg actcaaagcc ctgctggctt ttgaaaccgc
     2881 gcaaaacccc gaaaccgaac gcatcgtcgc cgacatttgc gccgacgtgc aaaagcgcgg
     2941 cgatgcggct ttgattgaat acaccaacaa attcgatcag acaaacgcta aaagcatcga
     3001 tgatttaata ctcacgcaag ccgatttgaa cgcggcgttc gagcgcattc cgaacgacgt
     3061 tcagacggca ttgcagaccg ccgcccgccg tgtcgaaagc taccaccaac gccaaaaaat
     3121 ggaatcgtgg agctacaccg atgaagacgg cacgctgttg ggacaacaaa tcacaccgct
     3181 tgaccgcgtc ggcatttacg tccccggcgg caaggcggcg tatccgagtt ccgtcatcat
     3241 gaacgccatg cccgcccacg tcgcaggtgt gaaagaaatc atcatggtcg tgccgacacc
     3301 aaaaggcgaa cgcaacgaca tcgtacttgc cgccgcatac gtcgccggcg taaccaaagt
     3361 cttcaccgtc ggcggcgcgc aggcggttgc cgccctcgcc tacggcacgg aaaccatccc
     3421 ccaagtcgat aaaatcaccg gtccgggcaa cgccttcgtc gccgccgcca aacgccgcgt
     3481 gttcggcgtg gtcggcatcg acatggtggc ggggccgtct gaaatcctgg tcatcgccga
     3541 cggcacgaca cctgccgatt gggtggcgat ggatttgttc agccaggccg aacacgacga
     3601 aattgcccaa gccatcctca tcggcacgtc gcaagcgtat ctcgacgaag tagaagccgc
     3661 tatggaccgc ctgatcgaaa ctatgccgcg ccgcgacatc atcgaagcct cgctcggcaa
     3721 caggggcgcg atgatactcg ccaaagactt ggacgaagcc tgcgaaatcg ccaactacat
     3781 ttcccccgaa cacttggaac tgtcagtcga aaacccgcag gaatgggcga aaaaaatccg
     3841 ccacgccggt gcgattttca tgggacgcta caccggcgaa agcctcggcg actactgcgc
     3901 cggtccaaac catgtgttgc ccaccagccg aaccgcccgc ttttcctcgc ctttggggac
     3961 atatgatttc caaaaacgct ccagcctgat tcaggtttcg gaacagggcg cgcaaaaatt
     4021 aggcgaaacc gccagcgtgc tggcacacgg cgaaagcctg accgcccacg cccgcgcggc
     4081 agagttccgt atgaaataat gccgaaacgg cgtacaggca tattccaacc attaaggaaa
     4141 cacgatgaaa tccgtccgct ccttcatccg cgacgacata caagctatgt cggcatatca
     4201 gattgccgac gttccgcccg gctttgccaa actcgattcg atggaaagtc ccgtccaccc
     4261 ttttgccgga catgaaacgc tgttgcagga atggcaggca cggcttgccg ccgcgcccat
     4321 ccatctttac cccaatccct ccggcagcgg tttacaggaa gcattacgtt cggcgttcga
     4381 cattcccgac tgcgccgaca tcgcgctggg caacggttcg gacgaactga tacagttcat
     4441 cacgatgctg accgccaaac cgggcgcggc aatgttggca gccgaaccca gtttcgtcat
     4501 gtaccgccac aacgccgcgc tgtacggcat ggattatgtc ggcgttccac tgaacggaga
     4561 tttcaccctc aacctgcccg ccgtcctcga agccgtcagg aaacaccgcc ctgccctgac
     4621 ctttatcgcc taccccaaca accccaccgg cgtatgcttc acgcgtgccg aaatcgaagc
     4681 cgtcatcgaa gcttcagacg gcatcgtcgt cgtcgatgaa gcctacggcg cattcaacgg
     4741 cgacagcttc ctgccgcagg caggcaggat tcccaacctg atagtcttac gcaccctcag
     4801 caaaatcggt tttgccggac tgcgtatcgg ttatgcggca ggctgccccg aagtcatcgg
     4861 cgaactgcaa aaaatcctgc cgccctacaa tatgaaccaa ttgagcctga ccactgccaa
     4921 actcgccctg cggcactacg gcattatctc tgccaacatc gacagcctga aaaacgaacg
     4981 cgaacggatg ttcgccgaat tgggcaaaat atgccgtctg aacacctttt caagtcaggc
     5041 aaacttcatt accatacgcg tacccgatgc cgatttgttg tttgacacgc tcaaacaaaa
     5101 ccgcatcttg gttaaaaaac tgcatggcgc gcacccgctt ttggaacact gcctgcgcat
     5161 taccgtaggc agccccgcac aaaacgatgc cgttctcaac atcattcgcc aactttactg
     5221 ccaaccaacg gatttcctat gaatttgact aaaacacaac gccaactgca caactttctg
     5281 accctcgccc aagaagcagg ttcgctgtcc aagctcgcca aactctgcgg ctaccgtacc
     5341 cccgtcgcac tctacaaact caaacaacgc cttgaaaagc aggcagaaga cccagatgca
     5401 cgcggcatcc gtcccagcct gatggcaaaa ctcgaaaaac acaccggcaa acccaaaggc
     5461 tggctcgaca gaaaacaccg cgaacgcact gtccccgaaa ccgccgcaga aagcaccgga
     5521 actgccgaaa cccaaattgc cgaaaccgca tctgctgccg gctgccgcag cgttaccgtc
     5581 aaccgcaata cctgcgaaac ccaaatcacc gtctccatca acctcgacgg cagcggcaaa
     5641 agcaggctgg ataccggcgt acccttcctc gaacacatga tcgatcaaat cgcccgccac
     5701 ggcatgattg acatcgacat cagctgcaaa ggcgacctgc acatcgacga ccaccacacc
     5761 gccgaagaca tcggcatcac actcggacaa gcaatccggc aggcactcgg cgacaaaaaa
     5821 ggcatccgcc gttacggaca ttcctacgtc ccgctcgacg aagccctcag ccgcgtcgtc
     5881 atcgaccttt ccggccgccc cggactcgtg tacaacatcg aatttacccg cgcactaatc
     5941 ggacgtttcg atgtcgattt gtttgaagaa tttttccacg gcatcgtcaa ccacagtatg
     6001 atgaccctgc acatcgacaa cctcagcggc aaaaacgccc accatcaggc ggaaaccgta
     6061 ttcaaagcct tcgggcgcgc cctgcgtatg gcagtcgaac acgacccgcg catggcagga
     6121 cagaccccct cgaccaaagg cacgctgacc gcataaaaaa ccataccgtc tgaaacaccc
     6181 gcaggctttt cagacggtat cggaacagat aagattacac tacactacaa acagaaaagg
     6241 agtaaacatc atgtccgcaa acgaatacgc acaaatcggc tggataggct tagggcaaat
     6301 gggtctgcct atggtaacgc ggctcttgga cggcggcatc gaagtcggcg tatacaaccg
     6361 ctcgcccgac aaaactgccc ccatctccgc caaaggcgca aaagtttacg gcaacaccgc
     6421 cgaactcgtc cgcgactatc ccgtcatttt cctgatggtt tccgactatg ccgccgtgtg
     6481 cgacatcctg aacggagtcc gcgacggatt ggccggcaaa atcatcgtca acatgagcac
     6541 catctccccg accgaaaacc tcgccgtcaa agcacttgtc gaagccgcag gcggacagtt
     6601 tgccgaagca cccgtttccg gatcggtcgg gcccgccacc aacggcacgc tgctgattct
     6661 gttcggcggc agcgaagccg ttttaaaccc gctgcaaaaa atattttccc tcgtcggcaa
     6721 aaaaaccttc catttcggcg atgtcggcaa aggttcgggc gcgaaactcg tcttgaactc
     6781 gctcttgggc attttcggcg aagcgtacag cgaagcgatg ctgatggcgc ggcagttcgg
     6841 catcgatacc gacaccatcg tcgaagccat cggcggctcg gcaatggact cgcccatgtt
     6901 ccaaaccaaa aaatccctgt gggcaaaccg cgaattcccg cccgccttcg ccctcaaaca
     6961 cgcctccaaa gacctcaacc tcgccgtcaa agagcttgaa caggcaggca acaccctgcc
     7021 cgccgtcgaa accgttgctg ccagctaccg caaagcagtc gaagccggct acggcgaaca
     7081 ggacgtttcc ggcgtttacc tgaaactggc agaacactga ttgccttttc caaacacaat
     7141 gccgtctgaa catatttcag acggcatttt tatcacccca cgcttaaaat cagtcccgat
     7201 tatg
//
LOCUS       DAWGRZ010000107         7185 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000107 DAWGRZ010000000
VERSION     DAWGRZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.107"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(120..1034)
                     /locus_tag="WHT44_001470"
     CDS             complement(120..1034)
                     /locus_tag="WHT44_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ2533643.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WHT44_001471"
     CDS             1290..2759
                     /locus_tag="WHT44_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ2533644.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="WHT44_001472"
     CDS             3011..3382
                     /locus_tag="WHT44_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533645.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGNDVMQMLAPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /locus_tag="WHT44_001473"
     CDS             3455..4864
                     /locus_tag="WHT44_001473"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ2533646.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDENIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPA
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGEMVVCLETALAAKFDATIREAVGDAAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4910..5704
                     /locus_tag="WHT44_001474"
     CDS             4910..5704
                     /locus_tag="WHT44_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533647.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGTKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSAGIAVPELLAVRK
                     NALMFGNLEGIPLDAQIRQEAEAGKSDAWLAGLEAIARVHKKQQFLSQAFARNMMWDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5892..6668
                     /locus_tag="WHT44_001475"
     CDS             5892..6668
                     /locus_tag="WHT44_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ2533648.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            6744..6850
                     /locus_tag="WHT44_001476"
                     /pseudo
     CDS             6744..6850
                     /locus_tag="WHT44_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(6921..7064)
                     /locus_tag="WHT44_001477"
     CDS             complement(6921..7064)
                     /locus_tag="WHT44_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533649.1"
                     /translation="MENNKVKSDYAAKQESLGKVDENKNETMKIPPQVGTHMLGFSFI
                     FKC"
BASE COUNT         1751 a         1780 c         1897 g         1757 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcagaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta tcaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc agcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttatggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccgga ggcggtaaca cattcggcag cttagacggt ggcacaggca tgggcggcag
     3121 catcgtcaaa atggcggttg ggagccaatg ccgtgcggaa ttggacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gagtgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacccgaacg gatgaccggc aacgatgtga tgcagatgct
     3301 ggctccgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatgc cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcccccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgcgaaacc ttggacaaat ggcgcggttt
     3601 agcttatccc gaattggcgt ttgaaattat gcgcctgttt gttacggata ttccggagga
     3661 cgatttgcgc gacattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttgtcca acggcccgac
     3781 gctggcgttc aaagatatgg caatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcgaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaagggcgt gaacgtattt atgttgtcgc ccgacggtaa
     3961 aatgagcgcg ttccaacgcg cgcaaatgta cagcctgcaa gacgagaata tccacaatat
     4021 cgccgtgaag ggaatgtttg acgactgtca ggatattgtg aaggcggtgc agaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac ggtcaactcg atcaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgcaggcta tttcaacgcc acttcaagca atgacgaaac
     4201 cgtcagcttc tgcgtaccga gcggcaactt cggcaatgtt tgcgcgggac acattgccaa
     4261 acaaatgggc ctgcctatcc gccgcctgat tgtcgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggtg cataccgccc gcgcaacagc gcgcatactt atgtaacctc
     4381 cagcccgtct atggacattt ccaaagcgtc caacttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctgcggaaa tcaatacgct gtgggcggaa gtggctgcag gcaaaggctt
     4501 tgacctgcga tttgccttgg acaaagtcgg tggcaaatac ggttttacct ctggcaaatc
     4561 cacccacgcc gaccgcctcg ccaccatcaa gcaggtttac gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggtgaaat
     4681 ggtggtttgt ttggaaaccg cgttggcggc gaaattcgat gcgaccatac gcgaagccgt
     4741 cggcgatgcc gccattccgc gccctgccgc gctggaaggt ttggaaaacc tgccgcagcg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatattcc gtacaggggc attccgtccg aacttaagat tgaaagaaaa tgaccttacc
     4921 tatgcaggaa acccgttttt ccatactgct agacgaattg gcggcaaaac aggaggcagc
     4981 catcgccccc cacctgcttg ccgacggaac caaggtatgg atacgcaaag caggcaggca
     5041 caatgcacga tggcgttacg cgctgctcgg tatggttgcc cgatatttga aactgggggt
     5101 gttgaagccg gttcccagcc tcggcggcga gcctgccatt gcaaccgaat caaaacgcct
     5161 gtacgaattg cgctcggcgg ggatagccgt tcccgaattg ctcgccgtca ggaaaaatgc
     5221 ccttatgttc ggcaatttgg aaggcatccc gctcgatgcg caaatccgcc aagaagccga
     5281 ggctggaaag tcggacgctt ggcttgccgg tttggaagcc attgcacgcg tgcataaaaa
     5341 acagcagttc ctcagtcagg cgtttgcacg aaatatgatg tgggacggaa aaaacatcag
     5401 ctttttggat tttgaagacg atccttccga agtcctgacc atagcgcaat gccaagcccg
     5461 cgattggctc tgctacattc attctaccgc gctaatattg aaaaacggcg gactgctgga
     5521 agctgctgcg gaaaaatggg gcggcgtatt gtcggatcag cctgccgaaa tacaaaagct
     5581 gattgccgcc accgtcaaac cgatcctccc gatacgcagg ctggaacacc cccgctgggg
     5641 acgggacgcg ctcaggctgg cagcctcgat ttccctgatt tcccttgccg atatgccgcc
     5701 ataagccgcc gcccgcccca cgatgccgtc tgaagccctt cagacggcat ataaccaacg
     5761 cgcatcaagc attgtcgcaa ccatccgttt cgtttagaat tttgacttta tcccgaattg
     5821 ggcagatatt ctcattctga cacacgtccc aaataaacac tccccatttc ttttaccgac
     5881 aggaaaccca aatggcagca ttcaataccc aaaaagtatt gtccgtacac cactggacag
     5941 acgcatattt tacctttacc tgcacccgcg acgaatcgtt gcgctttgaa aacggccaat
     6001 tcgttatggt cgggctgatg gtggacggca agccactgat gcgcgcatac agcgtcgcct
     6061 ccgccaactg ggaagaacac ctcgaatttt tcagcattaa agtccaagac ggcccgctga
     6121 ccagccgcct gcaacacctc aaagtcggcg acgacgtgtt aatcagcaaa aaaccgaccg
     6181 gaaccttggt tgccggcgac ctgaatccgg gcaaacacct ttacctgttg agcaccggta
     6241 ccggcatcgc ccctttcttg agcatcacca aagatcccga aatttacgaa cagtttgaaa
     6301 aaatcatcct cgtacacggc gtgcgctaca aaaaagattt ggcgtactac gaccgcttta
     6361 ccaaagaatt gcccgaacac gaatacctcg gtgacttggt taaagaaaaa ctgatttact
     6421 acccgattgt ttcgcgcgaa gaattcgaac accacggccg cctgaccgac ctgatggtga
     6481 gcggcaaact gtttgaagac atcggcctgc ccaaaattaa tcctcaagac gaccgcgcca
     6541 tgctgtgcgg cagccccgcg atgctgaaag acacctgcaa agttttggac gatttcggac
     6601 tgaccgtctc tccgaaaaca ggcgtacgcg gcgattacct gattgagcgc gcatttgtgg
     6661 accaataatc tgcttgaaaa tgccgtctga acggcatctg cgataaaaaa ccccgaatcc
     6721 tgccaatcag gattcggggt ttttatagtg aattaaattt aaattaggac aaggcggcga
     6781 gccgcagaca gtacaaatag tacggcaagg cgagccaaca ctgtaccggt ttaaatttaa
     6841 ttcactatat agtgggtaaa aattagaaat gcacatttcc gtcattcacg cgcaagtggg
     6901 aatccagacc tatccgttaa ttagcatttg aaaataaaag aaaaaccaag catatgggtt
     6961 cccacttgcg ggggaatctt catcgtctca tttttgtttt catcaacttt tcctaaactt
     7021 tcctgtttgg ctgcataatc agacttgact ttgttatttt ccatattgag cttctcttaa
     7081 tttgaaaaaa ttaacataaa catttaaaaa taaaacaaaa ttatataata aaaaaaataa
     7141 tgagaatata aaggataaaa agagagttaa gttatttaat cggac
//
LOCUS       DAWGRZ010000108         7161 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000108 DAWGRZ010000000
VERSION     DAWGRZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.108"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            267..488
                     /locus_tag="WHT44_001478"
     CDS             267..488
                     /locus_tag="WHT44_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533650.1"
                     /translation="MAVWRDYITLESHDLAEGYTYFFNENTDDCYVLKEDFINNERYE
                     KTELYSQKDKVILFPKFGEYDLVLNPDII"
     gene            675..947
                     /locus_tag="WHT44_001479"
     CDS             675..947
                     /locus_tag="WHT44_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533651.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNDMD"
     gene            1043..1909
                     /locus_tag="WHT44_001480"
                     /pseudo
     CDS             1043..1909
                     /locus_tag="WHT44_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            1928..2191
                     /locus_tag="WHT44_001481"
     CDS             1928..2191
                     /locus_tag="WHT44_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533652.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            2298..4220
                     /locus_tag="WHT44_001482"
     CDS             2298..4220
                     /locus_tag="WHT44_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ2533653.1"
                     /translation="MQLLAAEGIHQHQLNVQKSTRFIGIKVGKSNYSKNELNETKLPV
                     RVIAQTAKTRSGWDTVLEGTEFKTTLSGADIQAGVGEKARADAKIILKGIVNRIQTEE
                     KLESNSTVWQKQAGSGSTVETLKLPSFEGPALPKLTAPGGYIADIPKGNLKTEIEKLA
                     KQPEYAYLKQLQTVKDVNWNQVQLAYDKWDYKQEGLTGAGAAIIALAVTVVTSGAGTG
                     AVLGLNGAAAAATDAAFASLASQASVSFINNKGNIGNTLKELGRSSTVKNLMVAVATA
                     GVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNLEANILAALV
                     NTAHGEAASKIKQLDQHYIAHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEILGETLL
                     DGRDPGSLNVKDRAKIIAKAKLAAGAVAALSKGDVSTAANAAAVAVENNSLNDIQDRL
                     LSGNYALCMSAGGAESFCESYRPLGLPHFVSVSGEMKLPNKFGNRMVNGKLIINTRNG
                     NVYFSVGKIWSTVKSTKSNISGVSVGWVLNVSPNDYLKEASMNDFRNSNQNKAYAEMI
                     SQTLVGESVGGSLCLTRACFSVSSTISKSKSPFKDSKIIGEIGLGSGVAAGVEKTIYI
                     GNIKDIDKFISANIKK"
     gene            4229..4651
                     /locus_tag="WHT44_001483"
     CDS             4229..4651
                     /locus_tag="WHT44_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533654.1"
                     /translation="MKYMISFLKKTFELMSWVLVILIIGTFYDYYQIRQYAELEKKSI
                     SNILLYAQKEKFRLESKDKYMRGGYTKYKFIFSEYSNTTFLNFINDLKKDNYLPLDGY
                     GHGFLCRKGESISINIYPEINKFILVWGYPENLCADSN"
     gene            4688..5089
                     /locus_tag="WHT44_001484"
     CDS             4688..5089
                     /locus_tag="WHT44_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ2533655.1"
                     /translation="MRKLNNHDVHKRYQDRLEEDVEFTINYELPLSCLWSTIKDFSSD
                     FEEKTEAFFILFKELLRRGHLKLQRDGQIIGHTPEEWEQIFREVWPEYEIEPNPLPGY
                     APFDIGMWLTVEAPAYAVWIDPEDGSEYWAG"
     gene            5212..5832
                     /locus_tag="WHT44_001485"
     CDS             5212..5832
                     /locus_tag="WHT44_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533656.1"
                     /translation="MLGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAATVATAQGYV
                     SKTKIKIGQIELRVTAATDKQLLKAIGEGRDTTGKMTEQLFDSVAKQNGFRVLSGGKY
                     GGNNGFDHVWQAADGSVVLIVESKQIRNGTVQLNPNGAGGYTQMSEDWIRQVLDQLPD
                     GSPAKAAVFKANKNGTLKTAIAGVDRQTGKAVILPVKVPSKANIRR"
     gene            5835..6770
                     /locus_tag="WHT44_001486"
     CDS             5835..6770
                     /locus_tag="WHT44_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ2533657.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCLDSIDYVDERKGVP
                     LAAMQHIFMDVRAAASHAYLFEHDLKKFKQYAYVAGKLGVLLSVNSTDPEPFFFPCDM
                     LNIQNPMFLMLMSDSPQLREFLVRNIDNIANDTEAFINRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDQEIAPRDLIVYDPLPADEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
BASE COUNT         2268 a         1341 c         1662 g         1890 t
ORIGIN      
        1 tgaagaaaga tattttttat tgtgagcagt ggtcttatgg ttataagaaa cttcataagc
       61 ctttttctga gaaacaagct gaggaaaaac atcttaaagg ggagttatat actgccgtaa
      121 taggttcggc gacacaacct gaatatgtaa ttaccttgcg agaggaagta ggtttttttt
      181 cggtacattt tttcgataaa tttggaaggg attatttaac ccatcaattt caaaaatatt
      241 ccaattcgaa ttattatttt ctttctatgg ctgtatggag agattatata actttggaat
      301 ctcatgactt agcagaagga tatacttatt tcttcaatga aaatacggat gattgctatg
      361 ttttgaaaga ggattttatt aataatgagc gatatgaaaa aacagaatta tattcccaaa
      421 aagataaggt aattctattt ccaaagtttg gcgaatatga tttggtgtta aatccggaca
      481 ttatttaatt gagttttaag gccgtctgaa aaaatttcag acggctttta ttattgggtt
      541 tggaatctga ggataaagct gataaaaacc aggaaattat caggttgcta tatacgtatt
      601 gttgtacaga ctaaaggcag caatcaaatc actactgctt acccacaaaa ataaatcgat
      661 tatatggagt aatcatgaat aagagaatga aaatgtgtcc tgcttgtcaa caaggctatc
      721 tctaccattc gaaacctaaa tatcttcatg atgaaattat tctgtgtgat gaatgcgatg
      781 cagtatggct caaaggtatg aatatatttt atggagaata tgaaaaagat ttttattctt
      841 atgttccttt catggaatcc caaggtataa cgagtgaatg tatttgggaa ggagatttgt
      901 ttgatcatcc atattatgaa gatgaaaact caaatgatat ggattgatgg aaattttaag
      961 cctgcgtagg tacgattagc catcaaacgg cgtaatcata cgcaagatta tcaacagaga
     1021 gggctggcag cgatatacca cccacaagat tgcccatgcc atagcgggct gtgcggcagc
     1081 ggcggcgaat aagggcaagt gtcaggacgg cgcgattggt gcggtcgtgg gggagattgt
     1141 cggggaggct ttggttaaga ataccgattt cagcggtatg actgcttctg aaattgaaaa
     1201 agctaaagcg aatattactg cgtatgcaaa attggtagcc ggagcgactg taggtgttac
     1261 aggaggcaat gttgatgtgg cggcaaatgc ttccgaaaca gctgttaaaa ataatgcatt
     1321 agatattatt tgggatattg gcaacctcgt atgggacggc ggtaaatgga tttacgccaa
     1381 atctattggc gataagcaga tggctcgaga agcggcgatt gattttggtg tggatgccgc
     1441 cgcagctgcc gttccctttg ttccggcagg tgcgactaaa atcagccgag gcggggctta
     1501 tgttctgaag gcgggagacg aagcagttga tacggctaaa gccatacagg aaattcagaa
     1561 gcagaccgga atcaagctta cttatgataa ggttaataag gtttggacaa caccggcggg
     1621 gttagattat gggttagatg ctaagcatgg taataggatt aaacatgttt tagcccatac
     1681 aattccaaat ccaaacaaac ctgttcattc tgtttttaat gtgtcccgta aagaagtttt
     1741 gcctttggtt gatgaagctt ggagaatgaa aggaaatcct ttgccaaatg attcatccgt
     1801 atatcttgta gatatgaaga aacctattgg aacaaaagga gaaacaaaag tgcggattgt
     1861 tgtgcaaaaa ggaacaaata aaatcatttc tgcatatcct cagaaataat taagaaagga
     1921 atctcttatg gataaagaaa ttaaaatttg cccaagatgt gagcaaggct acctttatca
     1981 tgcaaagcct aaatatttct ctggggaggt cattttatgc gatgaatgtt atgctatgtg
     2041 gcttggggat atgaaaattt tttacggaca atatggaaaa gatttttatg attatcatga
     2101 gtttatgaaa gataaaggca tagaagaaat aaatatgtgg gaaggagagc tttttgatca
     2161 cccatattat gaggatgaaa aatttaaata attgattttc tgttccccga atttgggaaa
     2221 tacgatgata ttttaaaccc aaatattatt taaagtagca ataggccgtc tgaatatccg
     2281 tttttcagac ggcctcaatg caactgctgg cagccgaagg cattcaccaa caccaattga
     2341 atgttcagaa aagtacccgt ttcatcggca tcaaagtggg taaaagcaat tacagcaaaa
     2401 acgagctgaa cgaaaccaaa ctgcccgtac gcgttatcgc ccaaacagcc aaaacccgtt
     2461 ccggctggga taccgtactc gaaggcaccg aattcaaaac caccctttcc ggagccgaca
     2521 tacaggcagg ggtgggtgaa aaagcccgag ccgatgcgaa aattatccta aaaggcatcg
     2581 ttaaccgcat ccaaaccgaa gaaaagctgg aatccaactc gaccgtatgg caaaagcagg
     2641 ccggaagcgg cagcacggtt gaaacgctga agctaccgag ctttgaaggg ccggcactgc
     2701 ctaagctgac cgctcccggc ggctatatcg ccgacatccc caaaggcaac ctcaaaaccg
     2761 aaatcgaaaa gctggccaaa cagcccgaat atgcctatct gaaacagctt cagacggtca
     2821 aggacgtgaa ctggaaccaa gtacagctcg cttacgacaa atgggactat aaacaggaag
     2881 gcctaaccgg agccggagcc gcaattatcg cactggccgt taccgtggtc acctcaggcg
     2941 caggaaccgg agccgtattg ggattaaacg gtgcggccgc cgccgcaacc gatgcagcat
     3001 ttgcctcttt ggccagccag gcttccgtat cgttcatcaa caacaaaggc aatatcggta
     3061 acaccctgaa agagctgggc agaagcagca cggtgaaaaa tctgatggtt gccgtcgcta
     3121 ccgcaggcgt agccgacaaa atcggtgctt cggcactgaa caatgtcagc gataagcagt
     3181 ggatcaacaa cctgaccgtc aacctggcca atgcgggcag tgccgcactg attaataccg
     3241 ctgtcaacgg cggcagcctg aaagacaatc tggaagcgaa tatccttgcg gctttggtga
     3301 atactgcgca tggagaggca gcaagtaaaa tcaaacagtt ggatcagcac tacattgccc
     3361 ataagattgc ccatgccata gcgggctgtg cggcagcggc ggcgaataag ggcaagtgtc
     3421 aagatggtgc gatcggtgcg gcggtcggtg aaatccttgg cgaaacccta ctggacggca
     3481 gagaccctgg cagcctgaat gtgaaggaca gggcaaaaat cattgctaag gcgaagctgg
     3541 cagcaggggc ggttgcggcg ttgagtaagg gggatgtgag tacggcggcg aatgcggctg
     3601 ctgtggcggt agagaataat tctttaaatg atatacagga tcgtttgttg agtggaaatt
     3661 atgctttatg tatgagtgca ggaggagcag aaagcttttg tgagtcttat cgaccactgg
     3721 gcttgccaca ctttgtaagt gtttcaggag aaatgaaatt acctaataaa ttcgggaatc
     3781 gtatggttaa tggaaaatta attattaaca ctagaaatgg caatgtatat ttctctgtag
     3841 gtaaaatatg gagtactgta aaatcaacaa aatcaaatat aagtggggta tctgtcggtt
     3901 gggttttaaa tgtttcccct aatgattatt taaaagaagc atctatgaat gatttcagaa
     3961 atagtaatca aaataaagcc tatgcagaaa tgatttccca gactttggta ggtgagagtg
     4021 ttggtggtag tctttgtctg acaagagcct gcttttcggt aagttcaaca atatctaaat
     4081 ctaaatctcc ttttaaagat tcaaaaatta ttggggaaat cggtttggga agtggtgttg
     4141 ctgcaggagt agaaaaaaca atatacatag gtaacataaa agatattgat aaatttatta
     4201 gtgcaaacat aaaaaaatag gagttagtat gaaatatatg attagttttc taaaaaaaac
     4261 atttgaatta atgagttggg tgttagtcat actaataatt gggacatttt atgactatta
     4321 tcaaataagg caatatgctg aattagaaaa gaaatctata tcaaatatct tgctatatgc
     4381 ccaaaaagaa aaatttcgct tagagagtaa agataaatac atgcgaggag gatatacaaa
     4441 atataaattt attttttcag aatatagtaa tactactttt ttaaatttca taaatgacct
     4501 gaaaaaagat aattatttac cacttgacgg ctatggacat ggttttctat gtagaaaagg
     4561 agagtctata tcaatcaata tataccctga aattaataaa tttattttag tatggggata
     4621 ccctgaaaat ctttgcgctg attctaatta agtaagcaag aataggttat agggaaataa
     4681 aattcaaatg agaaaattga ataatcacga tgttcataaa cggtatcaag atcgtttaga
     4741 agaggatgta gagttcacta tcaactatga gcttcctttg agttgtttgt ggtcaaccat
     4801 caaagacttt tccagcgatt ttgaggaaaa aactgaagcg ttctttattc ttttcaaaga
     4861 gctgctgcgc agaggtcatc tgaaactgca acgcgacggg caaattatcg ggcatacgcc
     4921 cgaagaatgg gaacaaatat ttagggaagt atggcctgaa tatgaaatcg aacccaatcc
     4981 acttcccggc tatgccccat ttgatattgg aatgtggctt acggtcgagg ctcctgccta
     5041 cgccgtatgg atagatcccg aagacggtag cgaatactgg gcgggataaa ataccaatgt
     5101 ttggaataaa tcccgtctga aaaacagctt tttcagacag gatttattcc aattatcggt
     5161 gatatacaga gttttgtaca agcacagacc gctgccgatc acctgtttgc tttgctgggt
     5221 gtggttccgg gtatcggtga atcgatacag gcctataaag tagcgaaagc ggcaaaaaat
     5281 ttacaaggca tgaaaaaagc cttggacaag gcagcaaccg ttgccactgc acagggctat
     5341 gtcagcaaaa ccaaaatcaa aatcggtcaa attgaattaa gggttactgc agcaactgac
     5401 aaacaattgc tgaaagctat tggcgaagga agggacacga caggtaaaat gaccgagcag
     5461 ttatttgact ctgtagctaa acaaaatggc ttcagagtgc tttcgggcgg caaatacggc
     5521 ggaaataacg gttttgatca tgtatggcag gctgccgatg gtagtgttgt tttgattgta
     5581 gaaagtaagc agattaggaa cggtacggta cagctgaatc cgaatggtgc gggtggatat
     5641 acgcaaatga gtgaggattg gattagacaa gttttagatc aattacccga tggtagtccc
     5701 gctaaagctg ctgtcttcaa agcaaataag aacggcacat taaaaacagc aatagcaggc
     5761 gttgatcgtc aaacaggtaa ggccgttatt cttcctgtca aagttccttc taaagccaat
     5821 ataaggagat aacaatgggg cacaatatga tgaccaccca aaaatggtat gagcatatta
     5881 ctaatgtaat cataggcaat actgctaatt tcaatagcgg ttgccttgac tctatagatt
     5941 atgtagatga aagaaaaggc gttccgcttg cagctatgca acatattttc atggacgtta
     6001 gagctgcagc ttcccatgcc tatctatttg aacatgatct taagaaattc aagcaatatg
     6061 cttatgttgc aggaaagctg ggggttttgc tgagtgtaaa ttctacagac cctgaaccct
     6121 tcttctttcc ctgtgacatg ctcaacattc aaaatccgat gtttctgatg ctgatgagcg
     6181 acagcccaca gctgcgcgag tttctggtgc gcaatatcga caacatcgcc aacgatacag
     6241 aagcctttat aaaccgctac gacctcaacc gtcatatgat ttacaatact ctgctgatgg
     6301 tagagggtaa gcagcttgat cggttgaaac aacgtagcga gaaagtcttg gcgcatccca
     6361 cccctagcaa atggctgcaa aagcggttgt acgattaccg cttcttcctc gctttcgccg
     6421 aacaggatgc cgaggcgatg aaggccgcct tagagccgct tttcgataaa aaaaccgcgc
     6481 gtatggctgc caaagaaaca ttgtcctatt tcgatttcta cctgcagccg caaatcgtta
     6541 cctacgccaa aatcgcatcc atgcacggtt tcgatttggg catagatcaa gaaatcgcac
     6601 cgagggattt gattgtttac gatccgctgc cggcagacga atatcaagac atcttcgatt
     6661 ttatgaaaca gtatgacttg tcttacccgt atgaatatct gcaggattgg atagattact
     6721 atacgttcaa aaccgataag ctggtatttg gtaacgcgaa gcgagagtga gccgtaaaac
     6781 tctgagctcc tgttttatag attacaactt taggccgtct taaagctgaa agattttcga
     6841 aagctataaa ttgaagccct tccatagtac atagatctgt gttgtggcgg ggctttacca
     6901 cgctgattgc cggagaagaa ctcaacctgc tggcaaaaca aggcatgaga tctttgcaat
     6961 aacatgagtt gagacctttg caaaaaagcc cttccccgac atccgaaacc caaacacagg
     7021 atttcggctg ttttcgtatc aaatacctcc taattttacc caaatatccc cttaatcctc
     7081 cccggatacc ccataataga gcatccgctc cgccttttag gcggtagcga gcgtacttag
     7141 cctgttggcg gctttcaaca g
//
LOCUS       DAWGRZ010000109         7113 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000109 DAWGRZ010000000
VERSION     DAWGRZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7113
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.109"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(150..590)
                     /locus_tag="WHT44_001487"
     CDS             complement(150..590)
                     /locus_tag="WHT44_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533658.1"
                     /translation="MIKETLMRPIFLSFVLFPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
     gene            complement(831..1010)
                     /locus_tag="WHT44_001488"
     CDS             complement(831..1010)
                     /locus_tag="WHT44_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ2533659.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            complement(1016..1603)
                     /locus_tag="WHT44_001489"
     CDS             complement(1016..1603)
                     /locus_tag="WHT44_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ2533660.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTQEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            complement(1625..2371)
                     /locus_tag="WHT44_001490"
     CDS             complement(1625..2371)
                     /locus_tag="WHT44_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ2533661.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIYS
                     FIDRCYTETLQYFDREEWGRLKEGFVRDACAQTPYFQEIPGEFLQYCQSLPLLDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGFSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEDNSPGQNHLSA"
     gene            complement(2361..3203)
                     /locus_tag="WHT44_001491"
     CDS             complement(2361..3203)
                     /locus_tag="WHT44_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ2533662.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLENVDAERVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFSELEAEVAKIADYQTRAGKEC
                     RRAA"
     gene            complement(3319..3627)
                     /locus_tag="WHT44_001492"
     CDS             complement(3319..3627)
                     /locus_tag="WHT44_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533663.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            complement(3700..4149)
                     /locus_tag="WHT44_001493"
     CDS             complement(3700..4149)
                     /locus_tag="WHT44_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ2533664.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPRCRLKQD"
     gene            4494..5387
                     /locus_tag="WHT44_001494"
     CDS             4494..5387
                     /locus_tag="WHT44_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ2533665.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAVRKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            5490..6593
                     /gene="prfB"
                     /locus_tag="WHT44_001495"
     CDS             5490..6593
                     /gene="prfB"
                     /locus_tag="WHT44_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ2533666.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFRIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            6672..7031
                     /gene="rnpB"
                     /locus_tag="WHT44_001496"
     ncRNA           6672..7031
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WHT44_001496"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1797 a         1624 c         1886 g         1806 t
ORIGIN      
        1 gatggaagcc ggttttaaaa aataatgtgg ataactttgt ggataatttt cagggaatgg
       61 caaaatgccg tctgaaatgc tgatatttca gtatttcaga cggcattttt atgataaggt
      121 ttgtgggtaa gtttgtgtat aagttgcaat cagagttttt ttccgcatac ggtttcatat
      181 tgtttttcgg ttaatgtccc ggacaggatg ctcatcgggc ggagggaaga ggctgtgtag
      241 ttttgtgtgg aaatttccgt gttttttgta tcaaacaact gtagcgaact taagcggtat
      301 gttttgttgt tgcagtggat ttcccactcg gcaatggcag tcttgtatgc gggggtgttg
      361 gcaaaacgtt cttgttttag attggtaaca acttttttat cttggaaaat catcagattt
      421 ccgttttttc tcacgctgtc tttattgata tatgtatgaa tattgccgtt tgagattgtg
      481 ccgatatttt cccatcgggc agacttgtcc ggtgtgctgc aggcggttat caaaataggg
      541 aataaaacga aagataggaa gattgggcgc attagggttt ccttaatcat tgaaatctgc
      601 tcattcagaa agaacgggtt gggcgggagg ggtagcgccg tctgtgaaat aaggcaaata
      661 aagcaaatat ggcaaattga tgcagccttc gtattttgcg tatttataca ttccgtatgg
      721 ggaaatatgc tgcttttgaa gtgtgtatta tggatgaact gcagataaca gtaaatcggt
      781 ttgatttgtt tccgtcagct tatgccgtct gaagcctttt cagacggcat ttattttgaa
      841 gttctggttg tttttgccag tgatcttttg atggtttgaa gttgtctttt atattcacgg
      901 caatacggac agaacagcag gtgtgtgtat atggaaatct tctcgcccgg ggtggtttcc
      961 cggtcctgat gtttggaaag aagcagggcg atatcgcggc attttttcat aacgtttact
     1021 tcgggttttc ttggttgaac catttgattt gcaggcattg gcgcaatgat tctcgggcgc
     1081 ggtgcataat ggtgtggtag ttggacgtgc tgataccgca catttgttgt atttcgtcgg
     1141 atgaaaaacc gagtatttcc ttcagggtaa atacccgtgc ggtgttttca ggcaggttgt
     1201 ataggcagct ttgcagaatt ttttggaatt cgttgttgtt taatgatttt tccggagtgt
     1261 tccaatgttg cggctgccct tcctgcgtcc aatgcccgtt ttgggaaaaa tggctttcaa
     1321 atgcttcatc cagtagctcg tcatccagtg tggtaaagac tttcctctgc cttccgattt
     1381 gacgtaatgc gtcaataatt ttgtttttca atatggcaaa aagccagctg ttgacaagtg
     1441 ccctgccttg aaaactgtcg cctgcgctgt atgcggacag caatgtttcc tgcactaaat
     1501 cttcagccaa atcagggtgg tcgggcaact gaagccgcgc aaaatgcaga agcagtttac
     1561 gcgactctat taattcggca tcggtcaggt cgggtagcgg catagtatcg ggaacaaggt
     1621 aattttatgc ggatagatgg ttttggcccg gggaattgtc ttccatattt tcggatatgg
     1681 cggacaagga ggggatgata atcctttgtt cagtccagcc tgaccatttc ccaagcaaaa
     1741 tatttttcca attatcgtcc tcaggcataa attcgacaag ggtttgggcg agggtgtcaa
     1801 acgaaagcgc ggagaacccc attatttcta gcagcatcat atcgaagccg tccaatgttt
     1861 ggtacatcac atcatcttcg gcgtttcgcc atattaacaa ggctgtttcc gcttcatgca
     1921 aatcatcggt aacatcatat cgatattgcc ggataaaggc cgcaggggaa ggtgtgtatt
     1981 tgctgtcatt tgaataatga atgtcgggaa tatccggaat ttgagcaact tctgccagca
     2041 attgggtata ttcaaaatcc atcagtgcca aaatgccgtc taaaagcggc aggctttggc
     2101 aatattggag gaactcgccg gggatttctt gaaaataggg cgtttgggcg cacgcgtcgc
     2161 ggacgaaacc ttctttcaga cggccccatt cttcgcggtc aaagtattgc agcgtttcgg
     2221 tataacaacg gtcgataaag ctgtagatat tgttgcgtat caggcggata tagacgttca
     2281 gtcggtcttg cggcagaccg tctgcggctt cgcccccgcg tatggcttgg gcgaaacggt
     2341 gctggtattg ggcggaggtt tcaggctgca cggcggcatt cctttccggc acgcgtttga
     2401 taatcggcga ttttggcgac ttcggcttcg agttcggaaa aaggcgggaa attaaaatcg
     2461 cgttccaaca gggtgggcgg aatcgtcggc agcttggcat aggcaagttc gagcaagtcc
     2521 caaacagtcg gcaaaactgc cgcgccatgt gtatcaatca acaattccgg cgtttccacg
     2581 tcatgtccgg caatatggat atagcacacg cgctctgcat ccacattttc caaaaaagcc
     2641 tccggcgaca gcagaccgtg attgacggcg ttgacgtaga tattgttcac atccagatga
     2701 atgccgcaat cggcctcacg tgcgacggcg ttgaggaact cgacctcgtt catctcggca
     2761 agcggggaat gcagatagta ggacgtgttt tccacggcga tgcggcagcc caaacggtct
     2821 tgcacttcgc ggatacgccg cgccgtatga tgcaccattt cctcggtaaa gggcagcggc
     2881 aacaaatcgt aaagatgacc gccgtcgtgg cagtagctca aatggtcgga gaaaaacgtg
     2941 caatcgtaac ggcgcatcat ttctttgatg ccgtctatca aatcagtatc cagcggtgct
     3001 tgcccgccca gcgacataga caatccgtgc aacgccagcg gcagccgttc cgccacacgg
     3061 tcaaactgtt tgcgcgccca gccgcccatt ttcagccagt tttccggtgc ggcttcgata
     3121 aagcatatcg ggctgttttc ggaaagcgag agaaagtctt ccgccaagtc gcggcggtag
     3181 cccaagcctg cgtgttgaat catattttta cccggttcgg atggcacaaa agaatgatgt
     3241 gcttgttaaa aaatctttgt ttgccgttaa aaaagccgca ttttactgaa aaacgcggct
     3301 tggtttgaag gggtgggatt atttagaacc gcatttgcct tcgccgcatt tgccttcggc
     3361 agatttggcc ttggctttag atgctttggt gtgtttgtgg gtttttttta cggtcgcacc
     3421 gcatttgccc tcgccgcatt tgccttcgcc tgctttagaa ccagccgcgc cgcacgaacc
     3481 ttcggcagat ttggacgcgc cgcaagagcc atgggcggat ttatgaacgc ctgttgcgtt
     3541 gcttgccggt ttgttggcag caactgcacc ggcggccaaa gacagggata aagcaccggc
     3601 gagagcggca gcaatgtttt tgttcatgtc atttcctttt caaaatatca agttaacgag
     3661 ataaggttgg aatgtttaaa gtcagattcc acgactgaat caatcttgtt tcagacggca
     3721 tcgggggcaa aaccgttttt tcagcagcgt atccagcgac catccgcccg caccctggaa
     3781 aagcagcggg attaatacca cgatataaat taaagccatt ttataaccgt tgtcgcagac
     3841 attgtaaccc gaaccggcgt gaaccgcagc ccaagcgacg gcggtaacga ccatcagccc
     3901 caatgccgac agacgcgttg ccaaacccaa aagcaacaat acgggcagca aaagctccgc
     3961 atacatggcg agattccagt ttaacgcgtc cggcagcaag ttgaacggga atggaaactg
     4021 atcgttgatt tcggaaaacc aattctcccc gttccatttt tgcaaacccg attccaaaaa
     4081 ttcatatgcg gcaaacagac gcaaaaccag catagcggat gccgtctgcc aagttgttcc
     4141 gattttcata agtgttcctg ttttggttct gttgataccc cattagacgg attttgtcgg
     4201 aaaaactgac acccggcagc caatgattta acaagttaat gaatataaaa agaaagtatt
     4261 ttgatggaaa gaaagggaaa aaggcgggat atttggataa cggcggtgtt gtgagataat
     4321 tcccgccttg gatagtatgc cttcaaatat aatttccgta aaggaaaaaa aaaagaaata
     4381 ttaattttga tgatgactta acaaagcccc catttgcttg gctgtcaaag gggggtgtta
     4441 agaaaagcaa tccctctttt tgacggggtg cgatatataa aggaatggaa aatatggtaa
     4501 cgttttcaaa aatcagaccg cttttggcaa tcgccgccgc cgcgttgctt gccgcctgcg
     4561 gcacggcggg aaataatgct gtccgcaagc cggtgcaaac cgccaaaccc gccgcagtgg
     4621 tcggtttggc actcggtggc ggcgcatcta aaggatttgc ccatgtaggt attattaagg
     4681 ttttgaaaga aaacggtatt cctgtgaagg tggttaccgg cacatcggca ggttcgattg
     4741 tcggcagcct ttttgcatcg ggtatgtcgc ccgaccgcct cgaattggaa gccgaaattt
     4801 taggcaaaac cgatttggtc gatttaacct tgtccaccag tggttttatc aaaggcgaaa
     4861 agctgcaaaa ttacatcaac cgaaaagtcg gcggcaggca gattcagcag tttcccatca
     4921 aatttgccgc cgttgctact gattttgaaa ccggcaaggc cgtcgctttc aatcagggga
     4981 atgccgggca ggctgtgcgc gcttccgccg ccattcccaa tgtgttccaa cccgttatca
     5041 tcggcaggca tacatatgtt gacggcggtc tgtcgcagcc cgtgcccgtc agtgccgccc
     5101 ggcggcaggg ggcgaatttc gtgattgccg tcgatatttc cgcccgtccg ggcaaaaaca
     5161 tcagccaagg tttcttctct tatctcgatc agacgctgaa cgtaatgagc gtttctgcgt
     5221 tgcaaaatga gttggggcag gcggatgtgg ttatcaaacc gcaggttttg gatttgggtg
     5281 cagtcggcgg attcgatcag aaaaaacgcg ccatccggtt gggtgaggag gcagcacgtg
     5341 ccgcattgcc tgaaatcaaa cgcaaactgg cggcataccg ttattgacgc tatgccgtct
     5401 gaacgtgttt ccagaaggct ttatccatag aaaaaaatac ccgaatcccg tacaatacgc
     5461 ccctaaaatt ttgaaagaac aaaagaatca tggaagccga agtaatcaac cagctcaaca
     5521 ataccctgaa cgatttggaa aagcgcagcg aagacatccg agtttatatg gattatcagg
     5581 gtaagaaaga ccgattggaa gaagttatcg gtctttccga agaccccgaa ctttggaacg
     5641 acccgaaacg cgcccaagaa atcggcaaag agcgcaaaat cctcgaaggc atcgtgttga
     5701 cgctcgacaa catcgcttcg ggcatcgaag acaaccgcat gctgattgaa atgaccgtcg
     5761 aagaaaacga cgaagaaggt tttgccgccg tgcaggaaga cgtagcgggg ctggaaaaac
     5821 agatggcgga tttggagttc aaacggatgt tcaaccagcc cgccgacccg aacaactgct
     5881 ttatcgacat caccgcaggc gcgggcggta cggaagcgga agactgggcg ggtatgctgt
     5941 tccgtatgta cagccgctac gccgagcgca aaggcttcag aatcgaaatc cttgaagaag
     6001 acgacggcga aatcgcaggc atcaaccgcg ccaccatccg tgtggaaggc gaatacgctt
     6061 atggtttgct gcgtaccgaa accggcgttc accgcttggt gcgttactcg ccgtttgact
     6121 cgaacaacaa acgccatacc tcgtttgcct ccgtgttcgt ttaccctgaa atcgacgatt
     6181 ccatcgaaat cgaaatcaac cccgccgatt tgcgcatcga cacctatcgc gcatcgggtg
     6241 cgggcggtca gcacatcaat aaaaccgact ccgccgtgcg cattacccac gagccgacgg
     6301 ggattgtggt gcaatgtcaa aacgaccgtt cgcaacacgc caacaaagcc gctgcgatgg
     6361 aaatgttgaa gtccaaactg tatgaattgg aaatgcgcaa acgcaatgaa gagaaacagg
     6421 cgttggaaga aggtaaatcc gatgtaggtt ggggcagcca aatccgttcg tatgttttgg
     6481 attcctcacg tatcaaagac ttgcgtacag gctacgaagt cggcaacacc aaagccgtat
     6541 tggatggcga cttggacggc ttcatcgaag ccagcctgaa acagggcgtg tagtttaggc
     6601 tgtatgccgt ctgaaacctg taccgtttca gacggcattt ttgtgtttcg tgttataatc
     6661 cgcgccgcac acgggacggg cagacagtcg ccgcgtatcg cgtaaggcat acggggagga
     6721 aagtccgggc tccgcagggt agaatgccgg ttaacggccg ggcgcggtaa cgcgacggaa
     6781 agtggaacag aaagcaaaac cgccgatggc tgctttggca gcacaggtaa gggtgaaaag
     6841 gtgcggtaag ggcgcaccgc gcatttggta acaatatgcg gcaggccaaa ccccattcgg
     6901 agcaagacca aacagaacgc aatgacgctg cccgccgagc gttcgggtag gttgcttgag
     6961 cataccggta acggtatgcc tagaggaatg actgtccgag acagaacccg gcttaccgcc
     7021 tgtccggtgt gcatccgaca cgcaaatgtc gtctgaaacc tgaaatatgg ttcagacggc
     7081 atttttattt aggttgtgtt tttatagtgg att
//
LOCUS       DAWGRZ010000110         7079 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000110 DAWGRZ010000000
VERSION     DAWGRZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.110"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(268..2958)
                     /gene="glnE"
                     /locus_tag="WHT44_001497"
     CDS             complement(268..2958)
                     /gene="glnE"
                     /locus_tag="WHT44_001497"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ2533667.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWGEIRAEENEEELARQLRELRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKALVRPFVFRKYLDYGAYEAMRKLHRQISS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAELGIML
                     SEHVETLLAAYRFLRDVEHRLQYWDDQQTQTLPTSPEQRQLLAESMGFDSYSAFSDGL
                     NVHRNKVNQLFNEILSEPEEQTQDNSEWQWAWQDKPDEEGRRCRLKAHGFDAETVAAR
                     LDQIRHGHKYRHLSAHAQPRFDAVVPLFVQAAAAQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DDLKACGGDTEAQMDTLRRFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTSEIQTAFDRIRTEILTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNISADCGLIDKTLAGQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWREVFGEEAATV"
     gene            complement(3156..3368)
                     /locus_tag="WHT44_001498"
     CDS             complement(3156..3368)
                     /locus_tag="WHT44_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533668.1"
                     /translation="MGFWNGVAKAAKAVGEGMIEAGNEHKALKMEYAEKSSEELHEIV
                     KSDGFFKNSTREKSAAYAILKERGEV"
     gene            complement(3501..3974)
                     /locus_tag="WHT44_001499"
     CDS             complement(3501..3974)
                     /locus_tag="WHT44_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ2533669.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(4137..5144)
                     /locus_tag="WHT44_001500"
     CDS             complement(4137..5144)
                     /locus_tag="WHT44_001500"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ2533670.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTDNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPNPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVHGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGEDSADKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            5376..5612
                     /gene="acpP"
                     /locus_tag="WHT44_001501"
     CDS             5376..5612
                     /gene="acpP"
                     /locus_tag="WHT44_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ2533671.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            5768..7015
                     /gene="fabF"
                     /locus_tag="WHT44_001502"
     CDS             5768..7015
                     /gene="fabF"
                     /locus_tag="WHT44_001502"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ2533672.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLTGKSGIGAITRFDTS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPGIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGDSLRMIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
BASE COUNT         1599 a         1888 c         1920 g         1672 t
ORIGIN      
        1 aaaagtagaa tgcgctattt taatggaaac ggcggggttt taaacggttc ttatttgttt
       61 gttttgattt ttgcttggat aaaaaatccc atcattccca caaaaacaga agcctgaaat
      121 cccgtcattc ccgcgaaaga ggaatccggt ttttgggttt cagccattcc cgataaatcg
      181 ctttagctct gccgcaacct taaaacggca cggtacgaaa ataccgtctg aaacccagat
      241 tgtcaggctt cagacggcat tttttgttca gacggttgcc gcttcttcgc cgaacacttc
      301 ccgccacaat ttcctgacat tgccgtaatg tgccaacagt tcgccggtta cttcggtttt
      361 tgccgcgtcg cgcagtttgg tgttgtgctg ctgccggcgg tagaagcgat aggcggtgcg
      421 gctttgtccg gcgagggttt tgtcaatcaa accgcagtcg gcggagatgt ttaagagggc
      481 gatgttgccg tagttgtcca agagttgcgg atactggcgg gcatgggcaa gtatcagata
      541 ttggacgata aattcgacat cgaccacgcc accgcgcgcg tatttgacgt tgctgtcggc
      601 aggcgggtgg gtggggaaca ttttttcgcg catttcgatg atttcgcctg ccaaggcggt
      661 ttggtcgcgt tcggcggtga ggatttcggt gcggatgcgg tcgaaggccg tctgaatttc
      721 ggacgtgccg cagatgaagc gggcgcgggt aagggattgg tgttcccacg tccaggcgtt
      781 ttcgcgctgg tatttttcaa aggcggcgat gctgtgggcg aggaaaccgg cgtcgccatt
      841 agggcgcagg cgcaggtcgg tttcgtagag gctgcctgcg ccagtggcgg cggaaagcca
      901 gttggtcagg cggcgggcga ggcggctgta cacgtcgcct gcgtcggggt gggggtcgtc
      961 gtagagatag acgaggtcga ggtcggaggc gtagccgagt tctttaccgc cgagtttgcc
     1021 gtagccgacg acggcgaatt gcggtgtgtc gcggtgtttt ttgggcatgt ccgcccatgc
     1081 gcacagcagg gcggcggcga ggatggtgtc ggcgagggcg gagagttggt cggagaggga
     1141 ttctaccgtc cacagtccgg cgaggtcttg gacggcgaga cggaagactt gggcgtgctg
     1201 gaagcggcgc agggtgtcca tttgcgcttc agtatcgccg ccgcaggctt tgaggtcgtc
     1261 tgaaagggcg gcggcgagcg cctgccaatc aaacgcggta tccaaaagct gcgcgctgat
     1321 gagttcgtcc aacaaaatcg gatatttgtt cagatacgcc gccacccaag aactttggcc
     1381 cataatctgc gccagttgcg ccaaggtttg cggatgttcg ttgaggaagg cgagataggc
     1441 ggatcggcgg ctgatgtttt cgagaaaatc caacagccgc atcaatgtat cggtcgggtt
     1501 gctttgcgct gccgccgcct gtacgaacag cggcacaacc gcatcgaaac gcggctgggc
     1561 gtgtgcggaa agatggcggt atttatggcc gtggcggatt tggtcgagcc ttgcggcgac
     1621 ggtttcggca tcgaacccgt gcgccttcag acggcatcgc cgcccttctt cgtcgggttt
     1681 gtcctgccat gcccattgcc attcgctgtt gtcttgcgtt tgctcttcgg gttcgctcaa
     1741 aatttcgttg aacaactgat tgactttgtt ccgatgaaca ttgagaccgt ctgaaaaagc
     1801 ggaataactg tcgaaaccca tgctttcggc gagcagttgc cgctgttcgg gcgaggtcgg
     1861 cagggtttgg gtttgctggt catcccagta ttgcaggcgg tgttcaacat cgcgcaggaa
     1921 gcggtaggcg gcaagcaggg tttcgacgtg ttcagacagc atgatgccca gctcggcaag
     1981 cttcttcagc gtttcctgcg tgcctttcag ttgcagcgcg cgcatttgtc cgccgcgtat
     2041 catctggaaa atctgggcga taaattcgac ttcgcggatg ccgcccgcgc cgagtttgat
     2101 gttgtccgcc atgccttttt tgctgacttc gctgctgatt tggcggtgca gcttacgcat
     2161 cgcctcatac gcgccgtaat ccagatattt gcggaacaca aaggggcgca ccagtgcttt
     2221 gatgtcgttc ggatacggcg taaccacgcg acctttgcac cacgcgtagc gttcccattc
     2281 tcgcccctgt gtaatcaaat attgctccag cgcggtttcg ctcaatacca acgcgcccga
     2341 atcgccgtcc ggccgcagcc gcatatcgac gcggaacacc tgcccatcgg cggtaatgtc
     2401 gttcagcagc gcaatcagtt tctgcccgac tttggtgaaa aattcctgat tgccccgttc
     2461 gcgcctgccg tcggtgtcgc ctgattcggg atagacgaaa atcaaatcga tgtcggaaga
     2521 cacgttcaac tcatagccgc ccgccttgcc catcgccacc acgctcaaat gctgcggcga
     2581 tttggtataa cgcccgatcg gcgtgccgta catgtcccga taataggcgt aggcaaaatc
     2641 cagcgcggta ttgacggcaa aatcggcaaa cagcgtaatc gtgcgggtta cttcgttcaa
     2701 atcgctgata cggttgatat cgcgcacgat aatctgcgac accacataac ggcgcaactc
     2761 gcgcaactgc cgcgccaatt cttcctcgtt ttcttccgcg cggatttcgc cccagtcggc
     2821 aaaggcttgg aaatccgctt cggtcaaaac cttgtcgagc ataggcagga atatttcggg
     2881 cttgagtttg ccgttgtcga gctggcgggc gaggaagagg gaatggcggc gggcggtgtc
     2941 gaggcggttg tcggacattt cggattccgt ttggaaggat gacgggaatg agatagtgga
     3001 ttaactttaa atcaggacaa ggcgagggga tgccgtaccg gtttaaagtc aagccactat
     3061 atcatagcaa ccttcgatgt cgtctgaaac cttgccccga tattcggacg gatgcctacc
     3121 aataaaaaac agcggcaaat gccgccgttt cctgttcaca cctcgccacg ctcttttaaa
     3181 atagcataag ccgcactttt ctcccgtgtg gaatttttaa aaaaaccatc actcttgacg
     3241 atttcatgca gctcctcact tgatttctcc gcatattcca ttttcaacgc cttatgctca
     3301 ttgccggctt caatcattcc ctctcccact gcttttgctg cttttgccac accattccaa
     3361 aaacccataa tcatgtcctc caatcgtttc aaataaggtc gttgtttctg tcaagaaaca
     3421 acgacagttt ataaactgtc ggaaaacaaa atgccgtctg aaggccggat aatgcttcag
     3481 acggcatcgg attgccggtt ttaaccctgc cctccgccgt tgttcaaata gtgttcccag
     3541 tgagaatata accgggcaat attttcttca taggtgtggg cagaaggaat ataaccgtct
     3601 ttgggcggca tatacctgac aaatgattct atttcttccg gataatcaaa atctgcataa
     3661 attttttcaa cttcccccaa aggatcctca aattcttctt ttctatggaa tacatcgctt
     3721 aagaggacgt acagccaatt tttaggggaa taatccctca tttccgaagc caaatcttcc
     3781 agaacaggct ttaattcaag aatattggat ttgttgatta aagatagttt gaacacacgt
     3841 tcatcatgat ctgaaagcgt catttttcgg gcataagctg ccacatcagc ccaacccaag
     3901 tatttattcc cataccccca cagaatatcc ttccaagaaa gacggacttt ttgcgcggtt
     3961 aaatcaaggt tcatttttta ctccgattgc ctgctccgtg ttcacgcatc ggtttgcctt
     4021 atttcaaaca gggcggcgcg taagggattt tcaaggggcg tcaggcggaa aaaggaaatg
     4081 aaaatgccgt ctgaacggca aaacgtgcgt tcagacggca ttttgggcgg atcgcgtcat
     4141 cgcgccaaag ccttcaaaca ttctttgacc aatgccggac ctttgtatat caatccgctg
     4201 tacacttgga cggcggtcgc gcccaagcgg attttatctg ccgagtcctc gccttccata
     4261 atgccgccta cgccgataat cggcagcttg ccgtctatgt ggtctgccaa cagcttcaac
     4321 acccgattac ttttttcatg aacgggcagc ccgctcaaac cgccctgctc gcctgcgagc
     4381 ggatggctgc cgagacttga tttgtcgatg gtggtattgg tagcgatgat gccgtccatt
     4441 tcgacggatt tgacaacgtg ggcgatgtct tcgatttgtg cttcatccaa atcgggggcg
     4501 attttgacgg cgagcgggac gtatttcccg tgtacagagg caagctgtgc ctgtttgttt
     4561 ttcaaagcct caagcaatgc gctcaactcg tcgccacctt gcagcgcgcg gaggttttta
     4621 gtgttgggcg aggaaatatt gacggtaatg taacttgcgt gtgcgtaggc tttttcaagg
     4681 cagattaaat aatcatcggc agcgttttcg atgggtgtaa ccgcgttttt accgatgttg
     4741 atgcccaata cgccactgaa tttacttttt tcgatgttgc gtatcatggt gtcgataccg
     4801 tggttgttga aacccatgcg gttgatgatg ccttggtgtt cgggaacgcg aaagaggcgc
     4861 ggctgcgggt tgccgggctg cgggttgggc gttaccgtgc cgatttcgat gaaaccaaag
     4921 ccgagcgcgc ccaatgcgtc gatgtattcg ccgtttttgt cgagtccggc ggcaagtccg
     4981 acagggttgg gcaaatccat acccatcaat tttacaggtt tggtacggtt gtcggttaca
     5041 ggaatcaaac ccaatttata aaccgtgtag agcgcgtcga gcgtgaagtg gtgggctttt
     5101 tcggcatcga gtgcaaacag gatgcgacgg gcaagtggat acatgacagg gctttctgct
     5161 tcagatggaa atgccgccat tttaaccgaa atcccgccaa acatacggca cacaggtcaa
     5221 acttgaacga caaaacccgc cgcacccaaa aatgtacaaa tcggcaaata ttgatataat
     5281 agtcattaga tagaaaactc tattaaaagt cggtacaata ccaccgattt atttccctta
     5341 aacaacctgc cgcaaggcat aaaggaacga ctgatatgtc aaacatcgaa caacaagtta
     5401 aaaaaattgt tgctgaacaa ctgggcgtaa acgaagccga cgtgaaaaac gaatcttcct
     5461 tccaagacga cttgggcgcg gattctttgg acaccgtgga gctggttatg gctttggaag
     5521 aagcattcgg ctgcgaaatc cccgacgaag atgccgaaaa aatcaccacc gtccaactgg
     5581 ctatcgacta catcaatgcc cacaacggct aaccggtcgt cgcccgacac aacagcctct
     5641 gctgcaccgc gcaatagagg cttttccctt atatggcaaa ctgtttgaac ccctgccgca
     5701 aattacagac ggcatctgca cgacagccgc atatgccccc attccgacaa ccaacagcga
     5761 ggttatcatg agtcagagaa gagtagtcat tacaggctta ggtcaggttt cccctgtcgg
     5821 caacactgtc gcagaggctt gggacaccct gctcaccggc aaaagcggca tcggcgcgat
     5881 tacccgcttt gacacatccg acatcaacag ccgtgtcgcc ggcgaggtgc gcggtttcga
     5941 catcggacaa tacatcagcg cgaaagaagc gcgccggatg gacgtattca tccactacgg
     6001 cattgccgcc gcattgcagg caatcgccga ttcgggtttg gacgatgtgg aaaacctcga
     6061 caaagaccgc atcggcgtga acatcggttc cggcatcggc ggactgcccg gcatcgaggt
     6121 caccggcaaa gccgtaatcg aaggcggcgc gcgcaaaatc aaccctttct ttatccccgg
     6181 ttctctgatt aatctgattt ccggacacgt taccatcctc aaaggctacc gcggcccgag
     6241 ctacgggatg gtttccgcct gcaccaccgg cgcgcacgcc atcggcgatt ccctccgtat
     6301 gattaaatac ggcgacgcgg acataatggt tgccggcggc gcggaaggcg caatcagcac
     6361 tttgggcgtg ggcggttttg ccgcgatgaa agccctctcc acccgcaacg acgaccccgc
     6421 caccgcttcc cgtccgtggg acaaaggccg cgacggcttc gttatcggcg aaggcgcggg
     6481 catattggtg ttggaagaat tggaacacgc caaaaaacgc ggcgcgaaaa tctacgccga
     6541 aatcgtcggc ttcggcatga gttccgatgc ttaccatatc accgcgccga acgaagaagg
     6601 ccccgccctt gccgttaccc gcgcgctgaa agatgccggc atcaatcccg aagacgtgga
     6661 ttacgtcaac gcgcacggca cgtccacccc cttgggcgat gccaacgaaa ccaaagccct
     6721 caaacgcgcg ttcggcgaac acgcctacaa aaccgtcgtc agctcgacca aatccatgac
     6781 cggccacctg ctcggcgcgg cgggcggcgt ggaggccgtg tacagcattt tggcgataca
     6841 cgacggcaaa atcccgccga ccatcaacat ttttgaacaa gacgttgaag ccggctgcga
     6901 tttggactac tgcgccaacg aagcgcgcga cgcggaaatc gacgttgcca tttccaactc
     6961 cttcggcttc ggcggcacca acggcacgct ggtcttcaaa cgcttcaaag gctgattccg
     7021 caaagccgcc gccgacatcg aaatgccgtc tgaaaccgtt tcagacggca tttttatag
//
LOCUS       DAWGRZ010000111         7061 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000111 DAWGRZ010000000
VERSION     DAWGRZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.111"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(98..1015)
                     /gene="thrB"
                     /locus_tag="WHT44_001503"
     CDS             complement(98..1015)
                     /gene="thrB"
                     /locus_tag="WHT44_001503"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ2533673.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYNAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLG"
     gene            complement(1052..1780)
                     /gene="ubiG"
                     /locus_tag="WHT44_001504"
     CDS             complement(1052..1780)
                     /gene="ubiG"
                     /locus_tag="WHT44_001504"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ2533674.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1932..3173
                     /locus_tag="WHT44_001505"
     CDS             1932..3173
                     /locus_tag="WHT44_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ2533675.1"
                     /translation="MPNKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSAAGGDVSLTVGQLVFFGILAFCVWASARLVDRFTGVLIGGMVLTF
                     IWAAGGLIADAKPSVLFDTQAPAGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSISGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            3323..4374
                     /locus_tag="WHT44_001506"
                     /pseudo
     CDS             3323..4374
                     /locus_tag="WHT44_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            4622..5185
                     /gene="gmhB"
                     /locus_tag="WHT44_001507"
     CDS             4622..5185
                     /gene="gmhB"
                     /locus_tag="WHT44_001507"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ2533676.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENTAPQAD"
     gene            5214..5957
                     /locus_tag="WHT44_001508"
     CDS             5214..5957
                     /locus_tag="WHT44_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ2533677.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVGI
                     LNWSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLVRKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            5954..6646
                     /locus_tag="WHT44_001509"
     CDS             5954..6646
                     /locus_tag="WHT44_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ2533678.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGV
                     CRKTTGIRFNWRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPKAKQW
                     LKIHGRELFALG"
BASE COUNT         1663 a         2325 c         1671 g         1402 t
ORIGIN      
        1 aaaatacccg gctatcgtct cattttcgtt ttaatcagcc ataaaaatgc cgtctgaaac
       61 cctttcagac ggcatttctg tcaaacgccg gacgcactca acccaaactc aacagcaggt
      121 tgcggaacgc gttcgggtct ttgataaacg tcatctcgcc cgcctgcgga aaatgaaaat
      181 ccaacaggcg cgacacccaa aaacggatgc agccggcacg ttgggcggtc gggaaatacg
      241 ccttttcttc ggcactcaag gggcgcacgc cctcataacc gccgataaac gcctttttca
      301 acgcctcatc caacttattg tccgccgtcc ttgcccaatc gttgaccgca atcgccaagt
      361 catacataaa attgccccgg caggcgtaat agaaatcgat gaagcccgat acctgaccgc
      421 cgtcaagcaa cacattgtct ttaaacagat cggcatggat gatgcccgaa ggcagatgat
      481 tgccgagatt gtccttcaac gcatcgattt cggaacacag cagtgcggca tcgtcttgcg
      541 acaggacggg cagcagccgg gcgcacgcct ccgtccacca cgcattgtaa cgcgggtttt
      601 ccatttccaa agggaaatcg gcggcggcaa ggtgcatttt cgccaacatc gcaccggtat
      661 gaaaacactg ctcagccgtc ggcagcgcgg tatccgaacc tttcaggcag gcaaccaggc
      721 aggcaggctt acccgccaaa acggaatcaa gccggccgtc tttgcgcgca accggcgcgg
      781 caaccgccac gcccttcata ctcaaatgcc ggttaagctc cagaaaaaac ggcagctctt
      841 cctgtttcaa cacttcaaac acggtcagca cataacgtcc cgaagtcgtc gtcagaaaat
      901 aattgctgtt ggtaatcccc tgcgcgatgc cctgcaggga aacaaattcc cccaaatcgt
      961 aaccgctcag gaagccgcgc atttcatcat cggaaacact ggtatagaca gacatatcaa
     1021 ctcacttgct caaaaacggg caaacccgcc gtcagaacgc cggacggcag gcaaacatat
     1081 aattcacatc ggtcgaatcg cacagggcat aagtttgcga caacacatgg taagtcatac
     1141 ccttcgtatc cgccacatcc aagcctgcct gacggcacat tcgcgccagc tcggcaggtg
     1201 cgatgaattt tttccagtcg tgcgtgcctt tggggacaaa cttcaacaga tattccgccg
     1261 ccacaatcag atgcaggtac gatttcgggt ttttattgat ggtggaaaaa aacaccatgc
     1321 cgtccggttt gaccagattg gcacaagcac gcacgatggc ggcgggatcg gggacgtgtt
     1381 ccatcatttc catgcacgtt accacatcga acgagtgcgg ttccgcctcg gcaaggtctt
     1441 ccacgcggat acattcgtat tcgatatcgg cgacattgtt caaagccgcg tgcaggcggg
     1501 cggtttccaa cgactgctcc gccatgtcga tgccctttac aaacgccgcg ccgcgccgcg
     1561 ccatactttc cgccaagatg ccgccgccgc agcccacgtc caaaacccgt ttgccgcaca
     1621 aatccgcgtg tccgtcgata taatccagcc gcagcggatt gatgtcgtgc aaggttttga
     1681 actcgcccga cttgtcccac catttgtcgg caatccggct gaatttggcg atttccccct
     1741 catcgacatt atattttttg tcggacattt tccctcccat ctgacgaacc gcccactcca
     1801 aaacccaaga ttatactatg gaacacaatg ccgtctgaaa gccttttcgg gcggcgcggc
     1861 gcaaatacct tacaaatcct tacactttac ggcataatgg cggctcgctt tttctggcag
     1921 aaagacaaaa tatgcccaac aaaacccctt cactgttcgg cggcgcgatg attatcgccg
     1981 gcacggtcat cggcgcaggc atgctcgcca acccgaccgc cacatccggc gtatggttta
     2041 ccggctcgct ggccgtgttg ctgtacacct ggttttctat gctttccagc ggcctgatga
     2101 ttttggaagt caacacccat tatccgcacg gcgcaagttt cgacacgatg gtcaaagacc
     2161 tgctcggacg cggctggaac atcatcaacg gcatcgccgt cgccttcgtt ttatacctgc
     2221 ttacttacgc ttatatcttc gtcggcggcg acctgaccgc caaaggctta ggcagcgcgg
     2281 caggcggcga cgtttcactc accgtcggac aactcgtctt cttcggcatc ctcgcctttt
     2341 gcgtatgggc atccgcacgc ttggtcgacc gcttcaccgg cgtccttatc ggcggcatgg
     2401 tattgacctt tatttgggcg gccggcgggc tgattgccga tgccaagccg tccgtcctct
     2461 tcgataccca agcccccgcc ggcacaaact actggattta cgccgccacc gccctgcccg
     2521 tctgcctcgc ttccttcggc ttccacggca acgtctccag cctgctcaaa tactttaaag
     2581 gcgacgcgcc caaagtggct aaatccatct ggacgggcac actgattgcg ctggtaattt
     2641 acgtcctctg gcaaaccgcc atccaaggca acctgccgcg caacgagttc gcccccgtca
     2701 tcgccgccga agggcaagtc tccgtcctca tcgaaaccct gtccaaattc gcccaaaccg
     2761 gcaatatgga caaaatattg tccctgtttt cctatatggc gatcgccacc tcgtttttag
     2821 gcgtaacgct cggactcttc gactacatcg ccgacatctt caaatggaac gacagcatct
     2881 ccggccgcac caaaaccgcc gcgctgacct tcctgccgcc cctgatttcc tgcctgctct
     2941 tccccaccgg cttcgttacc gccatcggct acgtcggcct ggcggcaacc gtctggacag
     3001 gcatcatccc cgccatgctg ctctaccgtt cgcgcaaaaa attcggcgca ggcaaaacct
     3061 ataaagttta cggcggcttg tggctgatgg tttgggtctt ccttttcggc atcgtcaaca
     3121 tcgccgcaca ggtattgagc caaatggaac tcgtccccgt atttaaagga taaaggcaaa
     3181 atgccgtctg aagcccgccg gcggcttcag acggcattgc cgcaacaaac ggcaaccgta
     3241 ttccggcaca cagcgcatta ccctgcccct cacgcacaaa tcccgccccg acaaaccggg
     3301 acgcaaccat aaaggaacaa tgatgaagct caaaatagac attgcaacca acaacttcaa
     3361 acacggcggc ggcacggaac gctacacatt ggatttggta aagggtctga acagacaaaa
     3421 catcacaccg gccgtttatg cgacgaaatt tgatcacagc attcctgaat acgccctaat
     3481 cgaaccccat cttgtcgatc aacaccggac gctgaaaaaa ctacgctcat tcctcttttc
     3541 aagccggctc gctcaaacca gaaaaaacag tgccgccaaa ctgattgcct gccaccacgc
     3601 cgattacgcc gacctcctca tctgcggcgg cacacacttg ggctacctgc accatatggc
     3661 gcaaaaaccg aacctgctcg accgcctcgc catacgccgc aaccgcagca actacgccac
     3721 cgccaaactg attatggcgc attcccatat gatgcggcgc gaactggtcg gactgtacgg
     3781 cgttccccct gaaagaatcc aagtcgcccc ccccccgcag atacggaacg cttctttcca
     3841 caacccggag aaactgccga cctgcgcgcc aaatacggct ttgccgacca tgaaaccgtt
     3901 ttcctgttcc catcgaccgg ccacacgcgc aaaggtctgg aactgcttgc cgactttttc
     3961 gaacatacca gcctgcccgt caagctcgcc gttgtcggct ccccgcttcc ccgccctatg
     4021 aaaaacgtcg tcggactggg cttctgcacc gatatgcccg aactctaccg cgccgccgac
     4081 tttaccatta tggcttccct gtacgaaccc ttcgggctgg tcggcgtcga atccgtccta
     4141 tgcggcacac gcgtcgtcct ctccgaaaac atggcatgta cagaggtcat gaacgaagaa
     4201 gccggcttct ttttctcacg ccaaaacccg gaaaccctgg cgcaagccgt tgcccaagcc
     4261 gtcagcctta aaaaacaggg cggacaccgc ctgtccgacc cgatgcgggc actgaactac
     4321 aacccggctt tagacaaaca catcgggctg attcttgaaa tgcttgccgc ctgaccgcgt
     4381 ccccaaacgg cattgccccg caacttccgc gccgagactt ttgcagcgga aaatacgtcc
     4441 ggcagaaaat ccgccgttgc aggagcaggc aggaaaacat cggcaaccgc ccccgaaacg
     4501 ccgtacccgc gcattgcaag cggttgccgg aacaggcgcg ttatcgcgcg gcacaggcgc
     4561 atttccaccg atatttcagt ataatgccac ccccgacctg ccccaatcca aaggaaacgc
     4621 gatgaaactc atcattctcg accgcgacgg cgtcatcaat caggaccgcg acgacttcgt
     4681 caaatccgtt gacgagtgga tacctgtcga aggcagcatg gatgcggtgg catttctgac
     4741 gcaggcaggc tacaccgtcg ccgttgccac caaccaatcc ggcatcgggc gcaaatattt
     4801 taccgttcaa aaccttaccg aaatgcacgc caaaatgcac cgcctcgtcc gtcaggcagg
     4861 cggcgaaatc aacggcatct ggttctgccc gcacaccgat gccgacaact gcaactgccg
     4921 caagcccaaa ccgggtatga ttgaagacat catcggacgc ttcaacgccc aagcctcgga
     4981 aacctggctg gtcggcgaca gcctgcgcga tttgcaggca atcgatgccg tcggcggcaa
     5041 acccgcgctg gttctgaccg gaaaaggcaa aaaaacgctc tcccaacacg gacacgaatt
     5101 gcccgaacac acacaggttt tcgataccct gctcgatttc tcacaataca tcatgcagga
     5161 aaacaccgca ccgcaagccg actgaacata ccgcattccg acaaggcaaa accatgctca
     5221 tcatccgcaa cctgatttac tggctgatac tctgttccac cctgattttc ctctttccct
     5281 ttatgctgct cgcctcgcct ttccgggacg gggcgcacaa gatggcgcgg gtctgggtcg
     5341 gcattctcaa ctggtcgctc aaacacatcg tcgggctcaa ataccgcatc atcggcgcgg
     5401 aaaacatccc cgaccgcccc gccgtcatct gcgccaaaca ccaaagcggc tgggaaacgc
     5461 tcgcccttca ggacattttt ccgccgcagg tttacgttgc caaacgcgag ttgttcaaaa
     5521 tccccttttt cggctggggc ttgaaactgg tcaaaaccat aggcatagac cgcaacaacc
     5581 gccgcgaagc caacgagcag ctcataaaac aggggttggt gcgcaaaaac gaaggctatt
     5641 ggattaccat tttccccgaa ggcacgcgcc ttgcgcccgg aaaacgcggc aaatacaaac
     5701 tcggcggcgc gcgcatggcg aaaatgtttg agatggacat cgtccccgtc gccctcaaca
     5761 gcggcgaatt ttggccgaaa aactcctttc tgaaatatcc gggggaaatc accgtcgtca
     5821 tctgtccgac catcccgcac gcaagcggca gcgaagccga attgatggaa aaatgcgaac
     5881 atctcatcga aacgcaacaa ccgcttattt ccggcgcagg cccgtttgcc gccaaaatgc
     5941 cgtctgaaac cgcatgaccg cctttgtcca caccctttca gacggcatgg aactgaccgt
     6001 cgaaatcaag cgccgtgcca agaaaaacct gattatccgc cccgccggca cacataccgt
     6061 ccgcatcagc gtcccaccct gcttctccgt ctccgctcta aaccgctggc tgtatgaaaa
     6121 cgaagccgtc ctgcggcaaa cactggcgaa aacaccgccg ccgcaaactg ccgaaaaccg
     6181 gctgcccgaa tccatcctct tccacggcag acagcttgcc ctcaccgccc atcaagacac
     6241 gcaaatcctg ctgatgccgt ctgaaatccg tgttcccgaa ggcgcacccg aaaaacagct
     6301 tgcgctgctg cgggactttt tggaacggca ggcgcacagt tacctgattc cccgcctcga
     6361 acgccacgcc cgcaccacac aactgttccc cgcctcctcc tcgctgacct ctgccaaaac
     6421 cttctggggc gtgtgccgca aaaccacagg catacgcttc aactggcggc tggtcggcgc
     6481 accggaatac gttgccgact atgtctgcat acacgaactc tgccacctcg cccatcccga
     6541 ccacagcccc gccttttggg aactgacccg ccgcttcgcc ccctacacgc ccaaagcgaa
     6601 acagtggctc aaaatccacg gcagggaact tttcgcctta ggctgacgcg gaccggaccg
     6661 acccgccgct ttcagacggc atccgtgccg gaacaggcac gcgcccgccc gattcaaacc
     6721 gcgatgacgc tttgccgccg gttcggggca ggatggcggc acacacgccg tctgccgcgt
     6781 ttcatttcac accgctcttc cgaaacccga aacccgcccg gtccgacgtg cggtatgaaa
     6841 cgcttaagct gacgcgaagt cttttactga tttgcccgcg aaaatgccgt ctgaaaggtt
     6901 ttcggacggc atttttttgc gtttcccagg atggcggcgg attcgtaaaa ggcggtcagg
     6961 gtggattgta ggatgggttg agacctgccg aatccgccgc atctgccaaa tctaccgccg
     7021 tcattcctac gaaagtggga atctagaacg cggggttaag a
//
LOCUS       DAWGRZ010000112         7049 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000112 DAWGRZ010000000
VERSION     DAWGRZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7049
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.112"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            142..576
                     /locus_tag="WHT44_001510"
     CDS             142..576
                     /locus_tag="WHT44_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ2533679.1"
                     /translation="MNFALSVIMLTLASFLPVPPAGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(624..1967)
                     /locus_tag="WHT44_001511"
     CDS             complement(624..1967)
                     /locus_tag="WHT44_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ2533680.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1972..2625)
                     /locus_tag="WHT44_001512"
     CDS             complement(1972..2625)
                     /locus_tag="WHT44_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ2533681.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2776..3066
                     /gene="gatC"
                     /locus_tag="WHT44_001513"
     CDS             2776..3066
                     /gene="gatC"
                     /locus_tag="WHT44_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ2533682.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAGAPEVRNRLYIVPQVIEE"
     gene            3129..4574
                     /gene="gatA"
                     /locus_tag="WHT44_001514"
     CDS             3129..4574
                     /gene="gatA"
                     /locus_tag="WHT44_001514"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ2533683.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQTGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4571..5503
                     /locus_tag="WHT44_001515"
     CDS             4571..5503
                     /locus_tag="WHT44_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ2533684.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDNMVAEMKDSLKTEARGISDGIQTEKPPRRTA"
     gene            5546..6976
                     /gene="gatB"
                     /locus_tag="WHT44_001516"
     CDS             5546..6976
                     /gene="gatB"
                     /locus_tag="WHT44_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ2533685.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINQKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKGQEEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAHDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKLTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEATIAEIIEKHGLQQMTDTGEIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
BASE COUNT         1834 a         2119 c         1760 g         1336 t
ORIGIN      
        1 ttttgggaat ctgttttacc ccaatatata aagcaccata ttaaggcgga gtgtcttccc
       61 cactttgacc cgaacccgga aaagacaccg cccaagccaa tcctgatgct gccccgacag
      121 ccaaccatta aggaaatcct aatgaacttt gctttatccg tcattatgtt gaccctcgcc
      181 tctttcctgc ccgtcccgcc tgccggagcc gccgtcttta cttggaagga cggcggcggc
      241 aacagctatt cggatgtacc gaaacagctt catcccgacc aaagccaaat cttaaacctg
      301 cggacgcgcc aaaccaaacc ggcggtcaaa cccgcccaag ccgacgcagg gaagcgcaca
      361 gacggcgcgg cacaggaaaa caatcccgac actgccgaga aaaaccggca gcttgaggaa
      421 gaaaagaaaa gaattgccga aaccgaacgg cagaacaaag aagaaaactg ccggatttca
      481 aaaatgaacc tgaaggcggt gggaaattca aatgcaaaaa acaaggatga tttgattcgg
      541 aaatacaata acgccgtaaa caaatactgc cgttaatcgg ctctagcgca aacccgatgc
      601 cgtctgaagc ggcacggggt ttgtcatttc tgccagtagg ttttgacgtt gacgaactcg
      661 tacagcccga attcggacaa ttcgcgcccg taaccggaat ctttgactcc gccgaaaggc
      721 aggcgcaaat cgctgctggt atggcggttg ataaacaccg atcccgcctg tattttttcg
      781 gcaaaccgcc aagcgcgttc ggtatcggcg gtataaatgc aggcaccgag cccgaacggg
      841 gaatcattgg caagggcgat ggcatgttct tcgttttcgg cgcgcaaaat cagggcggcc
      901 ggcccgaata cttcttctct ccagacgcgg caggcaggat ttaccctgtc taaaaccgtc
      961 gcgggataaa accagcctcg cccttgtggg atttttccgc cggtcaggca taccgcgccg
     1021 tttgaaacgg catcttcaac ctgcccgtga accctgtccc gcaaatcttc gcggtgcagc
     1081 ggtgcaagcg tagtatcggg atgtttgggg tcgcccattt tcaatttagc gcattcggca
     1141 agaaacagcg tgataaaacg atcggctgcg gcttcggtta cgatgatgcg cttggcggcg
     1201 ttacacgatt gccccgcatc gcggaaacgg gaataacagg cttctgcggc ggcacgctcc
     1261 aaatcagcat cgggcatcac gataaaggcg ttgctaccgc cgagctccaa cacggttttc
     1321 ttaaggtttg cgcccgcgtg tgccgcaagg atgcgccccg tatgcgttga accggtaaac
     1381 gccattgcat cggtatcttc aaccgccttg agcgtgcccg cctcatccag ccacacgcct
     1441 gccagaggaa tgccgtctga agccaaatcg aacagtgcct gactgacgcg tgccacgctg
     1501 ggcgcgggtt tgacggcgca cgcgttgccc gcgcacatag cgggaacggc gaaacgcaat
     1561 acctgccaga cgggatagtt ccaaggcatg acggcaaaca ccacgcccaa aggctcgaag
     1621 cgcacctgac tcaaactcgc ctgcgtcgcg atggttttgt gggcaagcag ttcgggggca
     1681 aggcgggcgt aatagcgtat cagttcgata gacttgccga tttccgcacg gcattcgtgc
     1741 aagcagcgtc cgacttcctc acacaccatt tccgcaaaac gctctttctc cgcctccaaa
     1801 cggtcggcaa atttttgcag gcgcgcggca cgttcggtta cgcccagttg cgcgaacgcc
     1861 ccgccgcgca ttttcaaatc cgccagccgc cgttcaaact ccgcataatc ttgagcgggg
     1921 cggcggtaaa gcgtttcgcc cgtaaataca ttgacactgt gaaacatcga atcaacctgc
     1981 cagttgcggg aatatcgttt tcagtcccga cacaataatc tccaccgata ccgccgccag
     2041 catcataccc ataatgcggt ttaaaatcgt cagccccgtc gcgcccagca ggcggctgac
     2101 cttcccggca acgattaaaa tggcataaca aatcgcactg accaccaaac cggccgcgat
     2161 aatcaacgcg atgtcgccgt atgttttagc cgccgaagcg taaataatca cggtcgaaat
     2221 accgcccggg ccgatggtga tcggtatggc gatgggcacg acggcaatcg ctccggcatt
     2281 gcgggcgggg cgcgcctgcc ccgtttccgg ctgcgcgccg agattctgct tggcgggatt
     2341 gtcgttgccg ttcatcatcg aaatggcgat cagcagcacc aaaatcccgc cgccgacctg
     2401 aaacgaaccg acgctgatgc ccaaaacctt cagcagcgta ccgccgatca gcgcaaatac
     2461 cgcaatcacg gcaaacacgg caacggcggc cgtccgcgcg accttcctgc gctccttcgt
     2521 gctgtgcccg ttggtcaggt caaggtaaag cgacaacgcg ctaaacggat taatcagcac
     2581 caaaaaagcc acaatcagct tgccgatttc catgcccaat cccattattt ccccctcctt
     2641 caaacccgtg cggcaggcat ccgatgctgc aaattgccgc cgcaacggat ttttccgtta
     2701 taattaaaaa ttcaagcaat acgccccatc atacccgaac gacggtatct ttaccatcag
     2761 acaaggatgc ttttcatggc actgacactt gccgacgtag acaaaatcgc ccgactctcc
     2821 cgactgcacc tgactgcgga agaaaaagaa aaatcgcttc aagaattaaa cgacattttc
     2881 actatggtcg aacagatgca aaccattaac acagacggca tcgaaccgat ggcgcacccg
     2941 cacgaggccg ccctgcgcct gcgcgaagac gaagtaaccg aaaccgaccg cgccgccgaa
     3001 tatcaggcgg gtgctccgga agtacgcaac cgtctgtaca tcgtaccgca agttatcgaa
     3061 gaataatccg aatatgcttc agacggcatc agcaataccg cccgaagccc tttaaggatg
     3121 gaagatttat gacccaatac acattgaaac aggcaagcgt cctgttgcag tccaaacaga
     3181 tttccgccgt cgaactggca agcgcatacc ttgccgccat cgccgaaaaa aatcccgccc
     3241 tcaacggcta tatcaccatc gaccaagata aaacccttgc agaagcccgt gccgccgacg
     3301 aacgtatcgc gcagggcaac gcctccgcgc ttaccggcgt acccgtcgcc tacaaggata
     3361 ttttctgcca aaccggctgg cgcagcgcgt gcgcttccaa aatgctcgac aacttcatct
     3421 ccccctacac cgccaccgtc gtccaaaacc tgctcgacga aggtatggta acgctcggcc
     3481 gcaccaatat ggatgagttc gctatgggtt cgaccaatga aaactcattc tacggtgcag
     3541 ccaaaaaccc atggaatctt gagcacgtcc ccggcggttc gtcaggcggt tccgccgccg
     3601 tcgttgccgc gcgcctcgcc cctgccgcgc tcggttcgga caccggcggc tctatccgcc
     3661 aacccgcatc gcactgcggc attaccggca tcaaacccac atacggcacg gtttcccgct
     3721 tcggtatggt cgcctacgcc tccagcttcg atcaaaccgg cccgatggcg caaactgccg
     3781 aagactgcgc gattctgtta aacgcgatgg caggtttcga ccccaaagac tccaccagcc
     3841 tcgagcgcga aaaagaagac tacacccgcg atttgaacca accgctcaaa ggtttgaaaa
     3901 tcggcctgcc caaagaatat ttcggcgaag gcaacagcgc cgatgttctg acggcattgc
     3961 aaaacaccat tgatttgctg aaagcccaag gcgcggaatt gattgaagtt tccctgccgc
     4021 aaaccaagct gtccatcccc gcctactacg tcctcgcctc cgcagaagcc agcaccaacc
     4081 tttcacgtta cgacggcgta cgttacggac accgtgccgc ccaattcgcc gatttggaag
     4141 aaatgtacgg caaaacccgc gccgaaggtt tcggcagcga agtcaaacgc cgcatcatga
     4201 tcggcactta tgtactgtcg cacggctact acgatgccta ctatctcaaa gcccaaaaac
     4261 tgcgccgcct cgttgccgat gattttcaga cggcatttgc acggtgcgac ctcatcctcg
     4321 cgccgaccgc acccactgca gccccaaaaa tcggagcgga tgcttcgccg gttgaaacct
     4381 acttgagcga tatctacacc atcgccgtca acctcgccgg actgcccgca ttgaccctgc
     4441 ccgcaggctt cagcggcggc ggactgcccg tcggcgttca gcttgtcggc aactacttcg
     4501 ccgaagccaa aatcctcggt gcggcgcatc aaatccaact caacagcgat tggcacggca
     4561 aacgacccga atgaagcaga accgcacctt taccttcccc gattttcgca ccgtttacag
     4621 ctatgcgcct ttatatcggc tgcaacattt aaaatacaca ttgcgaaaat ttttcggaaa
     4681 aaaagaaatt tacgccttcg agcagtttgt caacgcatcc cctatccgtc aggggctgtt
     4741 cctccactgc ccgcaaaatg cctatccgct gctgcgcgaa tttgttgaca ggcgttttaa
     4801 ctgcaaacgc cgtttagatg cgatgacggc agattttctc atggcggaaa aactgttcgg
     4861 cacagacatc ctgcaccaaa tggaagacta ccgcttccat ttggtcttgg cgcacctttc
     4921 agacggcatc agcttgtggc tcaaccgcaa cgacaactgc gtcgaagaag gcgcgtggtc
     4981 tttatctttg cgcgacgaag caggcaaccg gctgtatatg gcgactttcg cctttgtcgg
     5041 cacacacctg ctgacagcct ccgtacaagg gccggcgggt gaagaagcca aagacaccgt
     5101 ccgccgcata accaaacaac tccacggctt gcgtccccaa caactgatgg taaccgccct
     5161 gcaatatttc gccgccgtac tcggcttgga cggcgcaatg ggcattgcac aaaaacatca
     5221 ggtcaaactg cgctggaaac ttaaaaagcg cgtcaaaatg aattacgacg cattctggca
     5281 ggaatacggc gcaagtttgg aacgggacgg ctactggcat ctcccccaaa cccccgcccg
     5341 caaagacctt gccgacatcg aaagcaaaaa gcgttcgatg taccgcaagc gttatgaaat
     5401 gctggacaat atggttgcag agatgaaaga cagtctgaaa acagaagcac gcggcatttc
     5461 agacggcatc caaacggaaa aaccgccccg ccggacagcc tgacgcgaag actatcgaat
     5521 tgatatttta gagaaagaag ctcttatgac ctgggaaacc gtaatcggct tggaaatcca
     5581 cgtccaattg aacaccaaat ccaaaatctt cagcggcgca tcgaccgcat tcggcgcaga
     5641 acccaacgcg cacgccagcg tagtggaatg cgcgctgccg ggcgttttgc ctgtgatgaa
     5701 ccgtgaagtc gttgaaaaag ccatcaaatt gggtttggct ttagatgcga aaatcaatca
     5761 gaaaaacgtg ttcgaccgca aaaactactt ctatcccgac ttaccaaaag gttatcaaat
     5821 cagccagttg gacttaccga ttgtcgaaca cggcaaattg gaaatcgtag tcggcgacga
     5881 tgtgaaaacc atcaacgtaa cccgtgcgca catggaagaa gacgcaggca agtccgtgca
     5941 tgaaggcttg aacggcgcaa ccggtatcga cctgaaccgc gccggcacgc cgctgttgga
     6001 agtggtatcc gaacctgaaa tgcgttccgc cgccgaagcc gttgcctacg ccaaggcctt
     6061 gcacagcttg gtaacctggc tggacatttg cgacggcaat atggcggaag gctcgttccg
     6121 cgtcgatgcc aacgtatccg tgcgcccgaa aggtcaagaa gagttcggca cgcgccgcga
     6181 gattaaaaac ctcaattcct tccgtttctt ggagcaggcg attaattacg aagcggaagc
     6241 gcaaatcgag attttggaag acggcggcaa agtacagcag gcaaccatgc tgtttgatcc
     6301 cgaaaaaggc gaaacccgcg taatgcgcct gaaagaagat gcgcacgact accgctactt
     6361 ccccgaccct gatttgctgc ccgttatcat ttcagacgcc caaatgcaaa aagccaaagc
     6421 agaaatgccc gagctgccga aagaaatggc agcgcgtttc gtggcggatt acggcgtgtc
     6481 cgaatacgac gcgcgcctgc tgaccgcaag ccgtgcgcag gctgcctatt ttgaagaagc
     6541 cgccaaagaa agcggacaag gcaagctgac tgccaactgg atgaacggcg aacttgccgc
     6601 cgcgctgaac aaagaaggca tggaacttgc cgacagcccg attaccgccc cgcgcctcgc
     6661 cgcgctggtt ggcaaaatcg ccgacggcac attaagcagc aagttagcga aaaaagcctt
     6721 tgaagccatg tgggcagaac ccgaagccac cattgccgaa atcattgaaa aacacggttt
     6781 gcaacagatg accgacaccg gcgagattga agccatggtg gacgaagtgc tggcaaacaa
     6841 cgccaaagcc gtggaacagt ttaaatccgg caacgaaaaa gccctgaatg cgattgtggg
     6901 acaagtgatg aaggccagca aaggcaaagc caaccccgcg caggttcaag agctgattaa
     6961 agccaaactg gcttaatccg ttatcacaca ggtcgtctga aagcaaagtt ccaacgaagg
     7021 taaaacagga aataagcttt cagacggcc
//
LOCUS       DAWGRZ010000113         6872 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000113 DAWGRZ010000000
VERSION     DAWGRZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.113"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(166..477)
                     /locus_tag="WHT44_001517"
     CDS             complement(166..477)
                     /locus_tag="WHT44_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ2533686.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
     gene            1006..3285
                     /gene="nrdA"
                     /locus_tag="WHT44_001518"
     CDS             1006..3285
                     /gene="nrdA"
                     /locus_tag="WHT44_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ2533687.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKEARLGYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRAEIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            3343..4356
                     /gene="dcm"
                     /locus_tag="WHT44_001519"
     CDS             3343..4356
                     /gene="dcm"
                     /locus_tag="WHT44_001519"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ2533688.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            4349..5536
                     /locus_tag="WHT44_001520"
     CDS             4349..5536
                     /locus_tag="WHT44_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction enzyme"
                     /protein_id="HEZ2533689.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            5597..6730
                     /gene="nrdB"
                     /locus_tag="WHT44_001521"
     CDS             5597..6730
                     /gene="nrdB"
                     /locus_tag="WHT44_001521"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ2533690.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
BASE COUNT         2033 a         1451 c         1532 g         1856 t
ORIGIN      
        1 tctgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac tatattaagg
       61 ctgcggcaac gggaggacct caaacgaaat ggcgtaagga tagtttgttg tatctatgga
      121 aaatgccgtc tgaacctgtg ttcagacggc atattttata tgggattaac ggcggaaact
      181 gccgccgtcg aaatcatcga aagcctcata agccgcccgg cattggattt tctgttccgc
      241 ataaggcagg gagcggaatg ctttgcgcgc ctttttgttt tcgcgcaatt cctgcacgtt
      301 gccttgcgat cggtaccacg cggcacggcg ttccaaatat ttcctgcatt cggggtgcag
      361 catctgcttg tttgccttgc ggatatgttt ggtatcgcgg tgggcgggtt ctgacgtacc
      421 ggcagcggca gttgtcgata ccagtcccaa tattgcagac aacagtatgg caagcatctt
      481 tttgacagac atgacgatcc tttcagtttg tcattctttc cagtatagga tggaaggatg
      541 gaacgggcaa attgtcggca gtttcagact cattcagccg acaaaacttg ccgtttgata
      601 tgcagattca aataacacat taatctcaat aataaattcc aatcggtaag aaaatggaat
      661 ttgtcggcgg cggcgcggca aaatcatact ttgcaaaatt taacaatttg caggggcaga
      721 aaacaggaag ctttcctttt tcgtcggaaa atccttattt caccgccttg tagccggagc
      781 cggtcaaaag gcaaaaaatt tacccgtttt ttatcggtaa agaattatca gataaaacaa
      841 atattatagg aaaaatacga caggcgggtt ttatcgcgca ttgcctgaaa ctgaaaaata
      901 caaccgttgt caagactgga gaaaatgcca aaaatccact atattgtctg ccttaattta
      961 tttgaaaaga ctgtgtcttg aatatcaaga gtggaagagg aagcgatgaa tacaccgact
     1021 gatttgaaag taaccaaacg agacggaaga ttagaagcca ttgatttgga taagattcac
     1081 cgtgtcgtca cttgggcggc ggacggattg gaaaatgttt ccgtgtcgca ggtcgagttg
     1141 aaatcgcaca tccagttcta caacggcatc cgcaccgacg acatccacga aaccatcatc
     1201 aaagccgctg ccgatttaat ttcggaagat accccggact accaatacct tgccgcgcgt
     1261 ttggcgattt tccatcttcg taaaatagcc tacggcgagt acgagccgcc gcacctttac
     1321 gaccacgtta aaaaacttac cgatgccgga aaatacgaca ggcatatcct tgaggattac
     1381 agccgcgaag aatttgacga actgaacgcc tatatcgacc acgaacgcga tatgtccttt
     1441 tcctatgccg ctgtcaaaca gctcgaaggc aaatatctgg tacagaaccg cgttacccgc
     1501 caaatttacg aaacgccgca gtttttatat gttttggtgg cgatgtgcct tttcagcaaa
     1561 tacccgaaag aggcgcgctt gggttacgtc aaacggtttt acgatgccgt ttctacattt
     1621 aaagtatcgc tgccgactcc gattatgagc ggcgtgcgta cgcctacgcg ccagttctca
     1681 agctgtgtgc tgattgaatg cgacgatagt ttggattcca tcaatgccac taccagcgcg
     1741 attgtgaaat acgtttccca gcgtgcgggc atcggcatca atgccggacg tatccgcggt
     1801 ttggacagcg aaatccgggg cggcgaagcg cggcataccg gctgcattcc cttctttaaa
     1861 atgtttcagg cggcggtcaa atcctgttcg caaggcggcg tgcgcggcgg cgcggcaacc
     1921 ttgttctacc ccttgtggca tatcgaagcc gaaagcctgc tggtgttgaa aaacaaccgc
     1981 ggtgtggaag acaaccgtat ccgtcagctt gattacggcg tgcaaatcaa ccgcctgctg
     2041 tacacccgcc tgattaaggg cggcaacatt acgctgtttt cgcccaacga ggttccggga
     2101 ttgtacgaag cgttttttgc cgaccaagac gaatttgagc ggctctatac gaaatacgag
     2161 caagaccctg atatccgcaa gcgcatcatt ccggctgccg acctgttttc cacgctgatg
     2221 caggagcgtg ccggaaccgg gcgcatctac attcaaaacg tcgatcactg caatacgcac
     2281 agcccgttcg atccgcgcgt cgcgcctgtt catcagtcca acttgtgtat ggaaatcgcc
     2341 ctgccgacca aaccgctgga caatatcaac gatccgaacg gcgaaatcgc cctgtgtaca
     2401 ctgtctgcct ttaacttggg cgcattaaac agcttggacg aattggaagg gcttgccgac
     2461 ttgaccgttc gtgcgctcga tgcactttta gattatcagg gatatccggt agaagccgcg
     2521 cgtacctcta ctatgggccg ccgttcgctc ggcatcggcg tgattaacta tgcttattat
     2581 ctggcgaaaa acggtgtccg ctacagcgac ggttccgcgc tcggtctgac ccaccgtacc
     2641 tttgaagcca tacagtatta cctgctcaaa gcatcggcaa accttgccaa agaatacggt
     2701 gcgtgcacgc tctttaacca aaccgtttat tcgcaaggca aactgcccat cgacacttac
     2761 aaaaaagatt tggatgccgt ctgcggcgag cctttgcatt acgactggga aagcctgcgt
     2821 gccgaaatcg tcaaatacgg cctgcgcaac tctactctga ccgcgctcat gccgtctgaa
     2881 accagctctc aaatcgccaa cgccaccaac ggcatcgagc cgccgcgcgg attggtaacg
     2941 gtcaaagcat cgaaagacgg cattttgaaa caagtcgtgc cggagtttga aaccctgaaa
     3001 aatgcctatg aaaccctgtg gcagcttccc ggcaacgaag gctacctgaa acttgtcggc
     3061 gtgatgcaaa aattcgtcga tcaatcgatt tccgccaata ccgcctacga cccgggcaaa
     3121 ttcgaaggcg gcaaagtttc tatgaaacaa atgctcaaag acctgctgac cgcctacaaa
     3181 tacggcgtca aaaccctgta ctaccataac acccgcgacg gtgcggacga tacgcagacc
     3241 gatattcagg atgacggctg cgcgggcggg gcttgtaaga tttgatgaaa gggggagttt
     3301 tcagatggcc tttagattaa taaatcatct gaaatataaa atatgaaaaa taaaatacaa
     3361 aatcaattag ataatatagt taccttaaat aatgagaaat ttacttttat tgatttattt
     3421 gcgggaatag gtggttttcg cattgcaatg gagaatgttg gtgggcgatg tgtattttct
     3481 agtgaatggg atgataaagc ccgtcaaacc taccaagtaa attttaatga tattccttat
     3541 ggagatatta cattaaagga aaccaaagca gctattccaa gtaagtttga tgtattaaca
     3601 gcaggattcc cgtgtcagcc attctctata gctggtgttt caaagaaaaa aagcctagga
     3661 cgagaaacag gcttcttaga taaggcgcag ggaactctat tttttgatgt tgctgaaatt
     3721 attggaaaac atcgacctaa aatttttctt ttagagaatg tgaaaaacct tgtttcgcat
     3781 gacaaaggaa atacatttaa agtaattaaa gggactttag aagagcttga ctatcagata
     3841 ttttatcaag ttatgaatgc aaaatattat gttcctcaaa atagggagcg tatttttatt
     3901 gtaggttttg atagacaata ttttaataag gaaataaatt tcaattttcc ttccccacca
     3961 gaatcacaac caaaattaaa gcaaattttg gaagatgatg tagataattc ttttactctt
     4021 tctgataact tatggcttta ccttcaaaat tacgctaaaa aacataaggc aaagggtaat
     4081 ggatttggtt ttggattagt tgatttagat ggaatatcac gaactctatc tgcacgatat
     4141 tacaaagatg gttccgaaat actcatccct cagaaaggaa aaaatcctag gaagctgaca
     4201 cctagggagt gctcgcgttt aatgggattt cctaaagatt ttgttattga tgcagtatcc
     4261 aagacagctg catacaagca gtttggcaat tcaattgctg taccgttggt tcaagctatt
     4321 gctaaacaaa ttataaatga gttaaaaaat gaatgacttg gcagctcaga caatacaagt
     4381 agtaaataaa tccgatgact tagtagcttc agcaatacag acagcaaata aatcaattgc
     4441 agtatactgt cgttatattc gtcccaatga tgttggtact actggtagtc accaatcagg
     4501 attttatatt caaaaacatt tttcagacga cctctttgat gtagtttgcc aaaagggaac
     4561 aaataaaact atttcaatca aaattaattg gcaagatgga agcgtcacta atagcaattt
     4621 taaatattac ggccaaggca cgagaaatga ggcgagaatt acgggttttg gcaagaattt
     4681 tgaattttta agtgataaat atagtggttc cttattggta ttgtgcagag cttgttataa
     4741 agatttatta ttccatgctt ttgttttatc ttcagatgaa gatattgaaa tatttatcgc
     4801 agaaactaac attttaccag gaagtttata tttacctaaa aaacaagaag tagaggataa
     4861 cctaacaaaa ttattttcac tatttcctaa cttccccaaa actgaagaaa tggcagtttt
     4921 ggcaagggaa aatattgcat taaataaaac taacgttagt aatgtgttaa aacaatgggt
     4981 ttcaaaggaa tatgaattat tttccatttt tgaaaaacgt gaatttgaaa ttattaaatc
     5041 aaaaattact gatttggata gcttcattaa ttttgcacat tcttttacaa atcgcagaaa
     5101 agcaagagca ggaaaatctt tagagttaca tttatcaaga atatttgacg aattttcctt
     5161 aaaatttgag acgcaagcga agacagaagg gaaaaagaaa ccagattttt tatttcctgg
     5221 aagtgaagag taccatgcga tggatgaatc agggaatttc atatttcaaa ctgaaaagct
     5281 gacaatgcta ggttcaaaga ctacttgtaa ggatcgctgg cgccaagttt taaatgaagc
     5341 agatcgaatt cctcataagc atttatttac tctacaagaa ggtatatctg atactcaaat
     5401 tcaggaaatg agtgatgaga atttaacctt agttgtccct aaagaatccg taaaaacatt
     5461 tggaactttt gggaaaaccc atgttttaac tttagaaaat tttattaaat atataaagtc
     5521 tcagcaggtt agttaaaact tacagatcat ttttaattaa tatagagaat agattatgtc
     5581 ctgcgaacac ttaactatgt catacagcac ctttcccaaa accaaaaacg acgcgctgaa
     5641 tgagccgatg ttttttggtc agccggttaa tgttgcccgt tatgaccagc agaaatacga
     5701 agtatttgaa aaactgattg aaaaacaatt gtctttcttc tggcggcctg aagaaatcga
     5761 tgtctcgcgc gaccgtatcg actacgccaa tctgcccgaa cacgaaaaac atattttcat
     5821 cagcaatctg aaataccaaa cactgctcga ttccatccaa gggcgcagtc cgaatgttgc
     5881 cttgctgcct ttggtttcga ttcccgagtt ggaaacgtgg attgaaacgt ggagcttcag
     5941 cgaaaccata cactcgcgca gctataccca catcatccgc aatattgtga atgatccgtc
     6001 ggtcgtgttt gatgatattg tcgaaaacga atacattacc gcccgcgccg aagacattgc
     6061 ctgctattac gatgacttaa tcgaatacac ccagtattac aacctgttgg gcgaaggggt
     6121 gcacaatgtc ggcggcaaac ccgttaccgt gtctttgcgc gggttgaaga aaaaactcta
     6181 tctctgcctg atgtgcgtca acgtgttgga agccatccgt ttctacgttt cattcgcctg
     6241 ctcgtttgct tttgccgagc gcgagttgat ggaaggcaac gccaaaatca tcaaactgat
     6301 tgcccgcgac gaagccctgc acctgaccgg cacgcagcat atgcttaatc tgatgcgttc
     6361 tggtgttgat gattctgaaa tggcagaaat tgccgccgag ttgcaggacg aatgtttcca
     6421 actcttcaaa aaagcggcgg aacaggaaaa agaatgggcg gcatatttgt ttaaagacgg
     6481 ttcgatgatt ggtttgaaca aagaaatctt atcccaatac gtcgaatata ttaccaatct
     6541 gcgtatgcag gcggtggggt tgccggccgg atttgaaggc gcaaatcaaa acccgattcc
     6601 gtggattaat gcgtggctgt cgtccgacaa cgtacaggtc gcgccgcagg aagtggaaat
     6661 atcctcttat ttgatcggtc agatagattc tgaagtgaat acggatgatt tgggcgattt
     6721 tgagttgtaa atgttttttg aaaaaatgcc gtctgaagcc ggatgtttca gacggcattt
     6781 gtatgttgta tgatgggaaa aacagcgggt aggatcggtt attgttcctt ttatagtgga
     6841 ttatagtgga ttatagtgga ttaacaaaaa tc
//
LOCUS       DAWGRZ010000114         6854 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000114 DAWGRZ010000000
VERSION     DAWGRZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.114"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(110..469)
                     /gene="rplT"
                     /locus_tag="WHT44_001522"
     CDS             complement(110..469)
                     /gene="rplT"
                     /locus_tag="WHT44_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ2533691.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGTRENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(482..679)
                     /gene="rpmI"
                     /locus_tag="WHT44_001523"
     CDS             complement(482..679)
                     /gene="rpmI"
                     /locus_tag="WHT44_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ2533692.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(826..1347)
                     /gene="infC"
                     /locus_tag="WHT44_001524"
     CDS             complement(826..1347)
                     /gene="infC"
                     /locus_tag="WHT44_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ2533693.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(1365..3278)
                     /gene="thrS"
                     /locus_tag="WHT44_001525"
     CDS             complement(1365..3278)
                     /gene="thrS"
                     /locus_tag="WHT44_001525"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ2533694.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKATVAGRVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLIRIYKQFGFHDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVRDALGRSWQCGTLQLDFVLPERLNAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQLVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(3331..3407)
                     /locus_tag="WHT44_001526"
     tRNA            complement(3331..3407)
                     /locus_tag="WHT44_001526"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3371..3373),aa:Val,seq:gac)
     gene            3586..4701
                     /gene="tgt"
                     /locus_tag="WHT44_001527"
     CDS             3586..4701
                     /gene="tgt"
                     /locus_tag="WHT44_001527"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ2533695.1"
                     /translation="MLKFTLHKKDGLARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVIGQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRTGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQARFHENRARGTD"
     gene            4711..5793
                     /locus_tag="WHT44_001528"
     CDS             4711..5793
                     /locus_tag="WHT44_001528"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ2533696.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFFPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAKRMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHHDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGSWR"
     gene            6089..6490
                     /locus_tag="WHT44_001529"
     CDS             6089..6490
                     /locus_tag="WHT44_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ2533697.1"
                     /translation="MNTTRLPTALVLGCFCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKSKKN"
     gene            complement(6553..6843)
                     /locus_tag="WHT44_001530"
     CDS             complement(6553..6843)
                     /locus_tag="WHT44_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533698.1"
                     /translation="MAKYFTETGISVREHFDFFGEFVVSPAARSGDLALTYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVEGGRIDIGGFMLTAKEVDGGGNIGSMGLKVLR"
BASE COUNT         1743 a         1848 c         1591 g         1672 t
ORIGIN      
        1 aacgcaaatt tatttaaaaa aatagacttt aaagaaagaa aatcaatcac atagaatttc
       61 tgcaaacaaa gaaaaaagga agccgcagct tccttttcaa ttttttggat taagcagcca
      121 aagcagcttt ggctttttca accaattgtg caaaagcggc tttatcgaac acggccaaat
      181 cagccaatac tttgcggtcg atttcaatag aggcgcgttt cagaccgttc ataaatttgc
      241 tgtaagacaa cccgttttca cgcgtacctg cattgatacg gacaatccac aattgacgga
      301 attggcgttt gcgttggcgg cggtcacggt acgcgtattg accggctttc attaccgcct
      361 gcttggcaac gcggtaaacg tttttacgac ggccgcggta gcctttggct aacgcgaaga
      421 ttttttggtg acgggcacga gcggtaacac cgcgttttac gcgtggcata ttctaaactc
      481 cttaagcgta gggtaacatt ttagcaacag aagccaaatc gcgatcattt accatagagg
      541 taccgcgcag ttggcgtttg tttttggtgg tctttttagt caagatgtgg cgtttgaacg
      601 catgagcgcg tttcacaccg ccgttaccca gtactttaaa gcgttttttc gcgctagact
      661 tggttttcat tttaggcatg ggaaaactcc attcgttatc ggataaggca ttagggggtt
      721 ttaaaacatc ggtttcaaac acttgaaccc acaatgccac ggttttttgc cgcaattgaa
      781 aacttactcc gaagcggcaa tcggagtaag cggagaatta tagctttatt ttttcttcgg
      841 cgcaatcatc atcaccattt ggcgaccttc cattttggga aaggactcga tttgcgccac
      901 ttcagccaaa tcttctttta cacgttccaa aagttgcgcg ccgagttgct ggtgagccat
      961 ttcacggccg cggaaacgca atgtcacttt gactttatcg ccgtcggcaa ggaagcggtt
     1021 aatgttgcgc atcttgattt gataatcgcc ctcatccgta cccggacgga atttgatttc
     1081 cttgatttgc acctgctttt ggtttttctt ggcttcgtcg cgtttcttgg cctgctggta
     1141 tttgtattta ccgtaatcca tcagtttgca cacaggcggt ttagcagttg gggaaatctc
     1201 taccaaatcg acatcctgcc cttcggccat agccaaagct tcacgaactg aaacgacacc
     1261 aagctgttcg cctgactcac tgattaaacg cacttctttg gcggtaattt cgccgttgat
     1321 tcgtgcttcg cgttcttgag cgatgatgaa tactcctata aaaattaatg attgacgagg
     1381 gcatcagtga tttcttgctg caattgcgca atgaaatcat ccaaatccaa agaacccaaa
     1441 tcttctgctt tgcggcgtac cgccactttg ttttcctgct tctccttatc gccgacaacg
     1501 atttgataag ggaaacggta ttggctgttg tcgcggattt tgtaaccgat tttttcgtta
     1561 cgcaaatcca actcggcgcg gaatcctgcc gcctgcaatt tggcagccac ttcccgacaa
     1621 taatctgcct gattttcggt aatattcata attaccaatt gaaccggagc caaccataac
     1681 gggaatgagc ctgcatggtt ctcaatcaga atgccgataa accgctccaa agaacctaaa
     1741 atggcgcgat gcaacataac aggacgcgca cggtcgttgt tttcagttac atattcggca
     1801 ttcaaacgct ccggcaatac gaaatccagt tgtaatgtac cgcattgcca agaacgaccc
     1861 aaggcatctc tgacatgata ttcgatttta ggcccgtaaa acgcaccctc gcccggcaat
     1921 tcgccccatt ccacgccgca ggcagtcaat gcctcgcgca aaccctgctc tgccttatcc
     1981 cacacgtcat ctgaacctgc gcgtttttca gggcgaagag aaagcttgac ggatacatca
     2041 tggaaaccga actgtttgta gatgcgaatc aacaattcat tgaacgcacg agcctcgctg
     2101 acgatttgat cttcggtaca gaaaatatgc gcatcatcct gcacaaaacc gcgaacccgc
     2161 atcagaccgt gcagcgcacc gctcggctca ttgcggtggc aagaaccgaa ttccgccaaa
     2221 cgcatcggca aatctcgata cgaacgcaaa ccgttgttaa aaatttgaac atgacccgga
     2281 cagttcatcg gtttaaccgc atattcgcgt ttttccgaac tggttacgaa catattatct
     2341 ttgtagttgt cccaatggcc ggatttttcc caaaaggttt tatccatgat ttgaggcgtt
     2401 ttgacctctt tataaccggc ggcgttcagc tctttacgca tatgctgttc aatcacttgc
     2461 cacaaagccc agcctttagg atgccaaaac accatgcccg gcgcttcgtc ttgcaggtgg
     2521 aacagatcca attgcttgcc aagtttgcgg tggtcgcgct tttcggcttc ttcgatacgt
     2581 tgaatatagg cttttaattc gtcttttgtc gcccaagccg taccgtatat gcgttgcagc
     2641 atttcattat tgctgtcgcc gcgccagtat gcgcccgcca acttggtcag tttgaagttt
     2701 ttcaggaaac gggtattcgg aacgtgcggg ccgcggcaca tatcgacata ttcctggtga
     2761 tgatacatcc ccatcgcttc cacttcgggc atatcgtcaa tcaggcgcag tttgtattct
     2821 tcgccgcgct cttgaaaaat tttaatcgcc tccgcacgcg gagtcatgat tttgaccaca
     2881 tcatagtctt gggcaatcaa ttctttcata cgcgcttcaa tggcggcaac atcttccggt
     2941 gtaaacggtt tttccgtggc gatgtcgtaa taaaagccct cttcaatgac ggggccgata
     3001 accatttttg cattaggata gagttgcttg acggcatgcc cgacaagatg cgcgcaggaa
     3061 tggcggatga tttcgatgcc ttcctgatct ttcggagtaa tgatttgaac agcagaatct
     3121 tcaacaatcg ggtcgcacgc atcgaccaat ttgccgttta ccctgcctgc caccgtcgcc
     3181 ttcgccaaac cggcaccgat agacgcagca atttgagcca cggtaacggg ggattcgtat
     3241 tggcggactg agccgtccgg caaggtaata ttcaacatca aacgctccga aagattaaaa
     3301 aataacggca taaatgccgt tatgggaatt tggtaggcac gattggattc gaaccaacga
     3361 cccccaccat gtcaaggtgg tgctctaacc aactgagcta cgtgccttca aagaattttt
     3421 gcattttatc gggtcgtttt aatttttgca agaggtatgt gcgtttcttt atgcaacatt
     3481 gcggcaagat ttggggatgt cgtctgaaaa accgtgggaa atcatttata atgccgtctt
     3541 ttaaaatata tttcgacccc tgtcgcccaa ctgacattca atcttatgct taaatttacc
     3601 ttacacaaaa aagacggtct cgcccgacgc ggcacgctgg agttgaacca cggcaaaatt
     3661 gaaacgccgg tatttatgcc tgtcggtact tacggttcgg ttaaggcgat gaacccgcaa
     3721 aacctgcacg atatcaaggc acaaattatt ttgggcaaca cttatcattt gtggctgcgt
     3781 ccgggtttgg aagttatcgg gcaattcggc ggcctgcacg gttttatcgg ctgggacaaa
     3841 ccgattctga ccgactcggg cggttttcag gttttttctt tgtcggatat gcgcaagctg
     3901 acggaagaag gctgtacgtt caaaagcccg attaacggcg acaagctgtt tttatcgcct
     3961 gaaatttcca tgaaaatcca aacggttttg aactcggata tcgcgatgca gttggacgaa
     4021 tgcacgccgg gcgaggcgac gcgcgaacag gcgcgaaaat cgctgcaaat gagcctgcgc
     4081 tgggcggaac ggagcaaaaa agcctttgaa gatttgaaaa acccgaacgc gctgttcggc
     4141 atcgtgcaag gcgcgatgta tgaggatttg cgcgaagaat cgctgcgcgg tttggaacag
     4201 tttgattttc ccggccttgc cgtcggcggt ttgtccgtcg gcgaacccaa gcccgaaatg
     4261 taccgtatgc tgcgcgccgt cggtccgatt ctgccggaac acaagccgca ttacctgatg
     4321 ggcgtaggca cgccggaaga cctcgtgtac ggcgtggcac acggcatcga tatgttcgac
     4381 tgcgtgatgc ccacccgcaa tgcacgcaac ggctggctgt ttacccgttt cggcgatttg
     4441 aaaatcaaaa acgccaaaca caagctcgac aagcgtccga tagacgaaag ctgcacctgc
     4501 tacgcctgcc aaaacttcag ccgcgcctac ctgcaccatc tgcaccgcac cggcgaaatc
     4561 ttgggcgcac agttgaacac catccacaat ctgcatttct accaagtcat tatggcggaa
     4621 atgcgcgaag ccgtcgaaca aggcaaattt gccgactggc aggcgcgatt ccacgaaaac
     4681 cgcgcacgcg gcacagactg attccctgca atgccgtctg aagccgcttc agacggcatt
     4741 ttgccgcttt accctacccg cactattttt cagaaaccga tcatgcttcc attctttcca
     4801 gaaccctccc tttcctatac gcaacaaaac cgtaccgccg ttttgctgtt gaacctcggc
     4861 acgcccgacg cgccgaccgc gcaggcggta cgaccttatc tgaagtcctt tctgaccgac
     4921 cggcgcgtcg tcgaactgcc caaatggctg tggtatccca ttctgcatgg tctggtactg
     4981 acgctccgcc ccaaaaaaag cgcgcacgcc tatgaaaaaa tctggtttaa ggaaggttcg
     5041 cccttagaag tttacacagc acgccaagct gccgcgcttg ccaaacgtat gcccgacctc
     5101 atcgtccgcc acgccatgac ctacggcaat ccttcggtgg cagacgtatt gtcggaactg
     5161 aaggcgcaag gcgcgggcag actgctggtc atcccgatgt acccgcaata tgccgcttcc
     5221 agcagcggag cggcagtaga taaggtgtgc gaacaattac tgctgcaacg gaaccaaatg
     5281 agcgtccgca ccgtttcgcg cttttatgac gataccggct acattgatgc gatgaaaaac
     5341 cacatcctcc gatattgggc ggaacacggg cgcgggaaaa aactgatgtt gagttttcac
     5401 ggcgtaccgc agaagcacca cgacctcggc gacccctatc ccgacgagtg ccgccacacc
     5461 gccaaactgc ttgccgaagc actggaactg accgaagacc aatatgtcgt atcgttccaa
     5521 agccaattcg gcagggcaaa atgggttaca ccgagcacgc aggatttgtt cggcaaactg
     5581 cccaaacagg gggtaaccga gctggacgta ttttgcccgg gctttttggc agactgtttg
     5641 gaaaccatgg aagaaatcgc cctgatgggg cgggaacagt tttatgaagc aggcggcaaa
     5701 agctaccgct acatcccctg cctcaacgac aaccccgact ggatagatgc actcgtcgca
     5761 cttgccgaag aaaaccttgg cagctggcgt tgaaactgtt tgacccatgt ctaaaatgcc
     5821 gtctgaacgc cgtgttcaga cggcattttc ccgctacttt agcgtatgat ggcatctatg
     5881 ctggacaaca acatcaagtg caaccttata gtggattaaa tttaaaccag tacggcgttg
     5941 cctcgccttg ccgtactatt tgtactgtct gcggcttcgt cgccttatcc tgatttttgt
     6001 taatccacta tactactgat tcggcaaaaa aatctttcac ccgctacgca aaatcaacaa
     6061 caaggtttca aacatgaagg aaacaccaat gaacacaacc cgactgccga ccgccctcgt
     6121 cttgggctgc ttctgcgccg ccgcttctgc cgccgacaac agcatcatga caaaagggca
     6181 aaaagtgtac gaatccaact gcgtcgcctg ccacggcaaa aagggcgaag gccgcggaac
     6241 catgtttccg ccgctctacc gctccgactt catcatgaaa aaaccgcagg tgctgctgca
     6301 cagcatggtc aaaggcatca acggtacaat caaagtcaac ggcaaaacct acaacggatt
     6361 catgcccgca accgccatca gcgatgcgga cattgccgcc gtcgccactt atatcatgaa
     6421 cgcctttgac aacggcggcg gaagcgttac cgaaaaagac gtaaaacagg caaaaagcaa
     6481 aaaaaactaa acagacaaaa tgccgtctga aaccggcaat ccggcttcag acggcattca
     6541 aatcaaacct ttctaacgca gcactttcag ccccatagac ccgatattgc cgccaccgtc
     6601 aacctccttt gcggtcagca taaagccgcc gatgtcaata cggccgccct cgaccggctc
     6661 ctgactatcg gaacgcttat cgaaaagctc ggcaaggctc aaaccctctt cgcccgcttc
     6721 cagcctcaaa ccgtaagtaa gtgccaaatc acccgaacgt gctgccggcg aaacgacaaa
     6781 ctcaccgaag aaatcaaaat gctcacggac gctaataccc gtctcggtaa agtatttcgc
     6841 cattttatag tgaa
//
LOCUS       DAWGRZ010000115         6841 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000115 DAWGRZ010000000
VERSION     DAWGRZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.115"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(76..1422)
                     /gene="mnmE"
                     /locus_tag="WHT44_001531"
     CDS             complement(76..1422)
                     /gene="mnmE"
                     /locus_tag="WHT44_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ2533699.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPKP
                     RTATYADFTDTDGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1555..1710
                     /locus_tag="WHT44_001532"
     CDS             1555..1710
                     /locus_tag="WHT44_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533700.1"
                     /translation="MKKRNTYALLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1707..2138
                     /locus_tag="WHT44_001533"
     CDS             1707..2138
                     /locus_tag="WHT44_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ2533701.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARCFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISAILIL
                     LFAVFLVWSGYPATAASLAGGTVFALAGAFVIGRSRDQGKN"
     gene            2272..6645
                     /gene="app"
                     /locus_tag="WHT44_001534"
     CDS             2272..6645
                     /gene="app"
                     /locus_tag="WHT44_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ2533702.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGRNPDQHRFTYKIVKRNNYKAGTKGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGRKYIDQNNYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPRQNGKYSFND
                     DNNGTGKINAKHEHNSLPNRLKTRTVQLFNVSLSETAREPVYHAAGGVNSYRPRLNNG
                     ENISFIDEGKGELILTSNINQGAGGLYFQGDFTVSPENNETWQGAGVHISEDSTVTWK
                     VNGVANDRLSKIGKGTLHVQAKGENQGSISVGDGTVILDQQADDKGKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDIATTGNNNSLDSKKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLNDHWSQKEGIPRGEIVWDNDWINRTFKA
                     ENFQIKGGQAVVSRNVAKVKGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEK
                     TITDDKVIASLTKTDISGNVDLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNG
                     NLSLVGNAQATFNQATLNGNTSASGNASFNLSDHAVQNGSLTLSGNAKANVSHSALNG
                     NVSLADKAVFHFESSRFTGQISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSATDAPRRRSRRSRRSLLSVTPPTSVESRFNTLTVNGKLNGQGTFR
                     FMSELFGYRSDKLKLAESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLNFT
                     LQNEHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALI
                     AAGRDAVEKTESVAEPARQAGGENVGIMQAEEEKKRVQADKDTALAKQREAETRPATT
                     AFPRARRARRDLPQLQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFA
                     EDRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENT
                     FDDGIGNSARLAHGAVFGQYGIDRFYIGISAGAGFSSGSLSDGIGGKIRRRVLHYGIQ
                     ARYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPA
                     QHISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAA
                     AAKGPQLEAQHSAGIKLGYRW"
BASE COUNT         1832 a         1966 c         1668 g         1375 t
ORIGIN      
        1 caaacggatc ggattcccgt ttttatggga taacggaatg ttcagccgga caccaccacg
       61 atgctttcga tccgtttatt ttccgataca aaacctcgaa aaaatcacgc cgagcaggtc
      121 gtccgccgta aactcgcccg tgatttcgcc gcatgcgacc tgcgccaagc gcaagtgttc
      181 ggcaaacagc tcgatttgat ggttgccgca caatgccgcc agcgacaatt cttcctgcgc
      241 tgctttgagt gcgttgacgt gccgcgtccg cgccaaaaac aacccttcgc tttcgccctg
      301 ccaaccggcc tcgcgcaaca acgtccgttt cagcgcgtcc aagccgtcgc cggttttcgc
      361 cgacaacgcg atgacggttt ccgcgcccgt accgaacccg cctgccgcgt gtgcgtgcaa
      421 atcggatttg ctgtggattt cgatgcgttt caactccggc ggcaacgcgt ccaaaatcgc
      481 ccgtgtcttt tcattcaaac cctcgcgcgg atcgaccaac accagcgcga catcggcttc
      541 ggatacggct ttgcggctgc gttcgatgcc gatacgctcg accacgtcgt ccgtctcgcg
      601 caaacctgcc gtatcgacaa tatgcaccgg cacgccgtca atcaggatac gttccctgac
      661 cgcgtcgcgc gtcgttccgg caatatcggt aacaatcgcc acttcgtcgc ccgccaacgc
      721 gttcagcagg ctggacttgc ccacattcgg cgcgccgacc aatacgacat tcagaccttc
      781 gcgcaaaatc gcgccctgct gcgcgttggc aagcacatca tccacggcgc ggcgcaagcc
      841 gtccagtttg ccgcgtgcgt ctgccgcttc gagaaaatca atgtcttcct cgggaaaatc
      901 taacgtcgct tcgaccagca tccgcaaggt aatcaagtct tcgaccagac cgtgtatccg
      961 ccgcgaaaaa tcgcccttga gcgagcgcaa agccagacgc gccgccgaac ggctggatgc
     1021 gtcaatcaaa tccgccacgc cttccgcctg tgccaagtcc agtttgtcgt tcaaaaacgc
     1081 acgcttcgta aactcgcccg gttcggcaag gcgcgcgccc aattccaaac agcggttcag
     1141 cagcatatcc atcaccaccg gcccgccgtg tccctgaagc tcgatgacat cttcacccgt
     1201 aaaacttgcc ggtgcggcaa aaaacagcaa aagcccgctg tcgattgcct gtccgtccgt
     1261 gtccgtaaaa tcagcatagg ttgcggtacg cggcttgggc gttttcccgc acaaagcctg
     1321 cgccatcggc agcaggtttt tccccgatat gcgtatcacg cccacgccgc cgcgccctgg
     1381 tgcggtagcg actgccgcaa tcgttggaac gttatccgac ataaaacccc cgaaaattca
     1441 aaacagccgc gattatagca aatgccgtct gaagtccgac ggtttggctt tcagacggta
     1501 taaaaccgca aaaatgcttg ataaatccgt ccgcctgacc taatatataa ccatatgaaa
     1561 aaacgaaaca catacgccct cctgctcggt ataggctcgc tgctgggtct gttccatccc
     1621 gcaaaaaccg ccatccgccc caatcccgcc gacgatctca aaaacatcgg cggcgatttt
     1681 caacgcgcca tagagaaagc gcgaaaatga ccgaaaacgc acaggacaag gcgcggcagg
     1741 ctgtcgaaac cgtcgtcaaa tccccggagc ttgtcgagca aatcctgtcc gacgagtacg
     1801 tgcaaataat gatagcccgg tgtttccatt cgggaccgtt gccgccgccg tccgacttgg
     1861 cgcaatacaa cgacattatc agcaacgggg cagaccgcat tatggcaatg gcggaaaaag
     1921 aacaagccgt ccggcacgaa accatacggc aagaccaaac cttcaacagg cgcgggcaac
     1981 tgtacggctt catcagcgcc atcctgatac tgctttttgc cgtcttcctc gtatggagcg
     2041 gctaccccgc aaccgccgcc tcccttgccg gcggcacagt gtttgccttg gcgggtgctt
     2101 tcgtgattgg aagaagccga gaccaaggca aaaattaatg acaaatccta gggcgtgctt
     2161 catatccgcc cgaacgccga accgcacata taggcacatc ccgcgcgccg cccggaagcg
     2221 gaagccgcgc cctcccaaac aaacccgaat cccgtcagat aaggaaaaat aatgaaaaca
     2281 accgacaaac ggacaaccga aacacaccgc aaagccccga aaaccggccg catccgcttc
     2341 tcgcctgctt acttagccat atgcctgtcg ttcggcattc ttccccaagc ctgggcggga
     2401 cacacttatt tcggcatcaa ctaccaatac tatcgcgact ttgccgaaaa taaaggcaag
     2461 tttgcagtcg gggcgaaaga tattgaggtt tacaacaaaa aaggggagtt ggtcggcaaa
     2521 tcaatgacaa aagccccgat gattgatttt tctgtggtgt cgcgtaacgg cgtggcggca
     2581 ttggtgggcg atcaatatat tgtgagcgtg gcacataacg gcggctataa caacgttgat
     2641 tttggtgcgg aaggaagaaa tcccgatcaa catcgtttta cttataaaat tgtgaaacgg
     2701 aataattata aagcagggac taaaggccat ccttatggcg gcgattatca tatgccgcgt
     2761 ttgcataaat ttgtcacaga tgcagaacct gttgaaatga ccagttatat ggatgggcgg
     2821 aaatatatcg atcaaaataa ttaccctgac cgtgttcgta ttggggcagg caggcaatat
     2881 tggcgatctg atgaagatga gcccaataac cgcgaaagtt catatcatat tgcaagtgcg
     2941 tattcttggc tcgttggtgg caataccttt gcacaaaatg gatcaggtgg tggcacagtc
     3001 aacttaggta gtgaaaaaat taaacatagc ccatatggtt ttttaccaac aggaggctca
     3061 tttggcgaca gtggctcacc aatgtttatc tatgatgccc aaaagcaaaa gtggttaatt
     3121 aatggggtat tgcaaacggg caacccctat ataggaaaaa gcaatggctt ccagctggtt
     3181 cgtaaagatt ggttctatga tgaaatcttt gctggagata cccattcagt attctacgaa
     3241 ccacgtcaaa atgggaaata ctcttttaac gacgataata atggcacagg aaaaatcaat
     3301 gccaaacatg aacacaattc tctgcctaat agattaaaaa cacgaaccgt tcaattgttt
     3361 aatgtttctt tatccgagac agcaagagaa cctgtttatc atgctgcagg tggtgtcaac
     3421 agttatcgac ccagactgaa taatggagaa aatatttcct ttattgacga aggaaaaggc
     3481 gaattgatac ttaccagcaa catcaatcaa ggtgctggag gattatattt ccaaggagat
     3541 tttacggtct cgcctgaaaa taacgaaact tggcaaggcg cgggcgttca tatcagtgaa
     3601 gacagtaccg ttacttggaa agtaaacggc gtggcaaacg accgcctgtc caaaatcggc
     3661 aaaggcacgc tgcacgttca agccaaaggg gaaaaccaag gctcgatcag cgtgggcgac
     3721 ggtacagtca ttttggatca gcaggcagac gataaaggca aaaaacaagc ctttagtgaa
     3781 atcggcttgg tcagcggcag gggtacggtg caactgaatg ccgataatca gttcaacccc
     3841 gacaaactct atttcggctt tcgcggcgga cgtttggatt taaacgggca ttcgctttcg
     3901 ttccaccgta ttcaaaatac cgatgaaggg gcgatgattg tcaaccacaa tcaagacaaa
     3961 gaatccaccg ttaccattac aggcaataaa gatattgcta caaccggcaa taacaacagc
     4021 ttggatagca aaaaagaaat tgcctacaac ggttggtttg gcgagaaaga tacgaccaaa
     4081 acgaacgggc ggctcaacct tgtttaccag cccgccgcag aagaccgcac cctgctgctt
     4141 tccggcggaa caaatttaaa cggcaacatc acgcaaacaa acggcaaact gtttttcagc
     4201 ggcagaccaa caccgcacgc ctacaatcat ttaaacgacc attggtcgca aaaagagggc
     4261 attcctcgcg gggaaatcgt gtgggacaac gactggatca accgcacatt taaagcggaa
     4321 aacttccaaa ttaaaggcgg acaggcggtg gtttcccgca atgttgccaa agtgaaaggc
     4381 gattggcatt tgagcaatca cgcccaagca gtttttggtg tcgcaccgca tcaaagccac
     4441 acaatctgta cacgttcgga ctggacgggt ctgacaaatt gtgtcgaaaa aaccattacc
     4501 gacgataaag tgattgcttc attgactaag accgacatca gcggcaatgt cgatcttgcc
     4561 gatcacgctc atttaaatct cacagggctt gccacactca acggcaatct tagtgcaaat
     4621 ggcgatacac gttatacagt cagccacaac gccacccaaa acggcaacct tagcctcgtg
     4681 ggcaatgccc aagcaacatt taatcaagcc acattaaacg gcaacacatc ggcttcgggc
     4741 aatgcttcat ttaatctaag cgaccacgcc gtacaaaacg gcagtctgac gctttccggc
     4801 aacgctaagg caaacgtaag ccattccgca ctcaacggta atgtctccct agccgataag
     4861 gcagtattcc attttgaaag cagccgcttt accggacaaa tcagcggcgg caaggatacg
     4921 gcattacact taaaagacag cgaatggacg ctgccgtcag gcacggaatt aggcaattta
     4981 aaccttgaca acgccaccat tacactcaat tccgcctatc gccacgatgc ggcaggggcg
     5041 caaaccggca gtgcgacaga tgcgccgcgc cgccgttcgc gccgttcgcg ccgttcccta
     5101 ttatccgtta caccgccaac ttcggtagaa tcccgtttca acacgctgac ggtaaacggc
     5161 aaattgaacg gtcagggaac attccgcttt atgtcggaac tcttcggcta ccgcagcgac
     5221 aaattgaagc tggcggaaag ttccgaaggc acttacacct tggcggtcaa caataccggc
     5281 aacgaacctg caagcctcga acaattgacg gtagtggaag gaaaagacaa caaaccgctg
     5341 tccgaaaacc ttaatttcac cctgcaaaac gaacacgtcg atgccggcgc gtggcgttac
     5401 caactcatcc gcaaagacgg cgagttccgc ctgcataatc cggtcaaaga acaagagctt
     5461 tccgacaaac tcggcaaggc agaagccaaa aaacaggcgg aaaaagacaa cgcgcaaagc
     5521 cttgacgcgc tgattgcggc cgggcgcgat gccgtcgaaa agacagaaag cgttgccgaa
     5581 ccggcccggc aggcaggcgg ggaaaatgtc ggcattatgc aggcggagga agagaaaaaa
     5641 cgggtgcagg cggataaaga caccgccttg gcgaaacagc gcgaagcgga aacccggccg
     5701 gctaccaccg ccttcccccg cgcccgccgc gcccgccggg atttgccgca actgcaaccc
     5761 caaccgcagc cccaaccgca gcgcgacctg atcagccgtt atgccaatag cggtttgagt
     5821 gaattttccg ccacgctcaa cagcgttttc gccgtacagg acgaattaga ccgcgtattt
     5881 gccgaagacc gccgcaacgc cgtttggaca agcggcatcc gggacaccaa acactaccgt
     5941 tcgcaagatt tccgcgccta ccgccaacaa accgacctgc gccaaatcgg tatgcagaaa
     6001 aacctcggca gcgggcgcgt cggcatcctg ttttcgcaca accggaccga aaacaccttc
     6061 gacgacggca tcggcaactc ggcacggctt gcccacggcg ccgttttcgg gcaatacggc
     6121 atcgacaggt tctacatcgg catcagcgcg ggcgcgggtt ttagcagcgg cagcctttca
     6181 gacggcatcg gaggcaaaat ccgccgccgc gtgctgcatt acggcattca ggcacgatac
     6241 cgcgccggtt tcggcggatt cggcatcgaa ccgcacatcg gcgcaacgcg ctatttcgtc
     6301 caaaaagcgg attaccgcta cgaaaacgtc aatatcgcca cccccggcct tgcattcaac
     6361 cgctaccgcg cgggcattaa ggcagattat tcattcaaac cggcgcaaca catttccatc
     6421 acgccttatt tgagcctgtc ctataccgat gccgcttcgg gcaaagtccg aacacgcgtc
     6481 aataccgccg tattggctca ggatttcggc aaaacccgca gtgcggaatg gggcgtaaac
     6541 gccgaaatca aaggtttcac gctgtccctc cacgctgccg ccgccaaagg cccgcaactg
     6601 gaagcgcaac acagcgcggg catcaaatta ggctaccgct ggtaaccgcc ggatatgccg
     6661 aaaggggtct gacgatgcca ccgtgcgctg tcaaaccctt tttctgccgc cgccttgtgc
     6721 ctgctccatt gtctgataac cgtcaaattg atttttagcc catgtttggt gggtcgggga
     6781 aatctatatc ttcgtccgtg ccgaaatagt ctgagacctt tgcaaaattc ctttccctcc
     6841 c
//
LOCUS       DAWGRZ010000116         6725 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000116 DAWGRZ010000000
VERSION     DAWGRZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6725
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.116"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(63..1355)
                     /locus_tag="WHT44_001535"
     CDS             complement(63..1355)
                     /locus_tag="WHT44_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ2533703.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIALTERTERVRPQRV
                     EQNLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLMYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAEGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            1432..2400
                     /locus_tag="WHT44_001536"
     CDS             1432..2400
                     /locus_tag="WHT44_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533704.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSETVALATPVWQKMGMHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPCAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDGTNGGAQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRIGKIKRLDIATDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            complement(2601..4067)
                     /locus_tag="WHT44_001537"
     CDS             complement(2601..4067)
                     /locus_tag="WHT44_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ2533705.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFDNRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(4309..6390)
                     /locus_tag="WHT44_001538"
                     /pseudo
     CDS             complement(4309..6390)
                     /locus_tag="WHT44_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1433 a         2007 c         1718 g         1567 t
ORIGIN      
        1 tgccgtctga aaacctgttt gcaggttttc agacggcatt gttttcgtcg ccggttcaaa
       61 cttcaatccg attgcgacac ggtaaccggt atgccgcgca agcgcgcaag cagcgcgtcc
      121 gccttctgtt tctgcgcggc aaacgccgcc ttgtccgcct gcgtcaattc cggtgtcggc
      181 aatgcgaccg aaacaggatt gacgggctgc ccgttgatgc gcgcctcgta atgcaggtgc
      241 ggcccggtcg aacgcccggt cgaaccgaca aaaccgatga cctcgccgcc gcgcacattg
      301 ccttccgcct gcgaaaacgc gctcaagtgc gcgtacagcg tttccacacc gttggcgtgg
      361 cgtatcatca ccgcgttgcc gtatccgccc ttccggcctt taaaggtaat cacgccgtcg
      421 gcggaagccc tgaccggcgt tccctgcggt gcggcataat cgatgcccgt gtgcagccgc
      481 caagtgtgca ggatggggtg catacggtag ccgaacggcg aagaaatgcg cgtatagacc
      541 agcggctcga tgttgaagcc gcctttttcc tgcaacaccc tgccgtcttc atcataataa
      601 ttgccgcccc ctccgccttc cttgtccgaa cggtaataga acgcctgatg ccttgtgccg
      661 cccttaacga cttccgccgc caaaatatcg cccgccgcca cctgctgccc gtggaaatac
      721 aggctgtcgt acatcaggcg cacggcatcg ccttccttca aaccgtcaag gctgaagcgg
      781 ccggcgaaaa tcccgcttaa ggattcgcgg atttcgacgg gcacttccgc ccgcgccagc
      841 gaaccgcgcg ccgacgtttt gaccacgacc gaacgcagcg tcggcaaaac cttcatatcc
      901 gcctcagaag ccgaccgccg ccatatgccg cctttctttt ccaaagcgac cagattgcgc
      961 tcgccgtctt cgtcggtaaa aaactgcact tcgcgcgcgc cgccgtcgcc gccgaccaaa
     1021 acatgaaccg actggtcggc acgcaaatgc cgcaaatcgg cttcgccgcc atatttttcc
     1081 gtgattcggg caatctcgtc ccgcgccata cccgaacgcg ccagcacgtc cgccagcgag
     1141 tcgcccggct gcaccgcctc ctgcacccaa tatgccgtct gaacgccgct gccgccccaa
     1201 gacagcggcg gcagattttg ttccacgcgc tgcggtctga cgcgttccgt cctctctgtc
     1261 aaagcaatgt atgccgccga caccaaaata atcgaaacgg caagcgcgcg cagcgcgtat
     1321 ttccgatgtt ttgccgaaag tgggaagaca gccatattgt tcctttttga cggcgtttgc
     1381 gccgaatcct ctataatatc cgcccgatcc gcatttgtca gacatcaaac gatgaaactc
     1441 accctcgccc tcccctcgct caatttggat gaagacgaaa tccgaatacc tctatgcctg
     1501 cccgccttca acaaaatcct gcaatacggc tcgctgcacc gccaaagctg cacggcttcg
     1561 gcattttacg cgcgttattt gtggtgcggc agactggcgg aacgcgcggc gcaaagcctg
     1621 aatatgccgt ctgaaaccgt cgcgctcgcc acgcccgtct ggcaaaagat ggggatgcat
     1681 caggcaaatg tgctgacggc ggaatatttg gatgtcggta cggatgaagc ggaacggctc
     1741 tgccgcgatc tgtcggcgtt ttacggcgat attccgtggc gtttcgttcc cgtcctgccc
     1801 gaattatggc tggtttccct tccttgcgcg taccgctggg gcgcgaagcc cgttttggac
     1861 ttgggcggat tgttgggcgc ggacgatcag ccggacggag aagatgcgtt ggaatggctg
     1921 cgcgtccaaa ccgaaatcca aatgtggctg gcggcgcacc ccgtcaacca taaccgcaaa
     1981 aaacgcgggc tgcccgagtt gaacggctta tggctttggg acggcacaaa cggcggcgcg
     2041 caaggcggca cgcttttcgc cgatacggtt tggagccgtt tccaccccga ccgccgcgca
     2101 ttgcccgaca acttccgcgc ctatgaagaa acggcggcac acctgcccga cacgcaccat
     2161 atcctgttta tggacgattt gcgcctgacc gccctgacag gggacaggga gcggtatgcg
     2221 gcaatattgc agcagtggga agaacgctgg ttcgcaccgc tttacgaagc cgtccgcatc
     2281 ggcaaaatca aacggctgga catcgccacg gacggacaac acggcggtac gctgaccttc
     2341 aaacccgccg acaggtggaa attttggcgc ggcgcgaaaa cctttgacgg aatctggtga
     2401 acccgcccgc cgcaaccgcg acaatgccgt ctgaagcccg aatcgggttt cagacggcat
     2461 tttacctcgc atcgccgtta tgccggaaaa accggaatgc gtttccgcac acgcggcggc
     2521 acgcaaagca ccgcccgaaa gccgccgatg cccgccgtga taccgccgcc ggcagggttt
     2581 cctccgaaaa ggaacagcaa tcagaacgtt ttattaaact cgacaaacgc ccgatttttc
     2641 tcgtattcgt tgaacacatc gttactccgc gtttcgcggt gcgacaacgt caggcgcggc
     2701 gtgatgcctt tgaaatgcaa tgcccggtgc caaaggctca aggatgtgtt caattcttta
     2761 tccctgcgcc tttccccttt aaaaccgctg aaaaagccgg gtttttcata atgccgtttc
     2821 gccgcgccga ggcgcaacag cgaagacagg ccgctgccgc cccattcctg cccccaggca
     2881 aagcgcaggc cgtaacggtt gaaattgtcg ccccggtcgg cggggttgcg ctcgcggtaa
     2941 aaatccaaac cgcccatcca atattggcgc gcattccggt aaaacaccag cgaattggaa
     3001 atttgcaaat gggtattgtc ggaacgcgcc cggcgcgtat tcttcaaacg cccccactcc
     3061 gccgaagaca acgtttgcca tttcggggtt tgccaacggt tgaaataaag gcgtgcgccg
     3121 ttggtgtaag aataagcgtc gttgccgtag gtgcggcgtt cgtggaacac tgccagcccg
     3181 gcatctttgc gccggtcggc aaaaccgatg ccgccggaaa cgcctgccgt catatcgttg
     3241 aatttcttat tccccggata aaccctgccg gacacgtcgc cgcccgccgt cgtgtaccag
     3301 ccgtttttca gcgaccattt tttctccgcg ccgagccggt aattgaccgc cgtgccgtcc
     3361 acctgtttcg ggaaagtcca tttgccgtac tgctgccgtt tcggggcttg gttgatattg
     3421 tgttcgcggg tgacgctgaa gccgccattt accttccacg catcgcgttc gcgcaatgcc
     3481 ttgcggtaca gctcgacctg ctccatcagc tgcggcggca ggttttccgc cttcaggcgg
     3541 tcgaactggt ctgccgccgc ctcgttctgc ctgttgtcaa acaatgccgc cgccaaacgc
     3601 atacggacgg cgggcgcgtc gggttgggcg gcaatcaatt cccggtaatg ggaaatcgcc
     3661 tccttcaccc taccgtctgc ctgcgccaaa atcccttgtg cataaagtgc caacatctta
     3721 tcctgctgcg cctgttgtag gtaaatcggc aaaataacgc ggataccggc aatattgttt
     3781 gagaccactg cggaatacat cgcgcgggac aacaattcgg gatttttcag cagggtttct
     3841 ccgtcaatct gcaaaacttt tcctttttcc cgcacctgcc ccggtacctt ctccctgtcg
     3901 attggtttga cctccgcttc atgaagcctg aactcgggac ggcttctcaa atccggttcg
     3961 cgcggtgttt cttcggcata tgccgcgctt gccaataaag gcagcagcag catccatctg
     4021 tttcgtgcag gcataaatgt cttcccacaa aaataaaaaa taaccattat caatcggcgg
     4081 attgtatcag ttttttatgc cgctgttcca aaccctgccg cccgatgccg cgacaatgcc
     4141 gtctgaagcc agaatcgggt ttcaaacggc ggcaaaaaac cggcggtttt ccgccggttt
     4201 catcatgatg ccccgccctt cggggcggca gggttcggat tggtttgcgg ggcggcgcgc
     4261 ggggccgtgc cggtgtgccg cctgtgcgtc aaaacatttc ccgacgcatc aaaacagcca
     4321 tttgagcgac aatgcggctt ctttgtcgcc gtccgtcctt ttgccatatc cgatgcgtgc
     4381 ggacatatgg cctttccaac cggcttcaat accgaaccgc ccttcgagtg ccgtcctgcc
     4441 tgccagcgtc tgtttttcgc cgtccatttc cacgccgaaa gattttgacc tgtgcaaaac
     4501 attaaaagcg gcaaaaggct gaagattgac accgttacgc aaagcaaaac gggtttttgc
     4561 ccgaatgccg gcgcggcttt gccactgacc gctgccgagc agtccgaccg ccgtcccctc
     4621 gctgtcggta aagccgccgt ttacgcccaa gtaggtaaac tgcgcctgcg gttgtaggta
     4681 aaaccgcaca ttattgcctt ttccgacaat gccttccgcc acaagcgcgt tgtagccgcc
     4741 ttcgacagaa gccgtccaac ctttggtttt gtagcgttcc gcacggtttt catcattgat
     4801 gcggtgtttg aaacgttggt attgcaacca gccgtccaaa tacgcacccg tttgtttatc
     4861 gcgcaactga tgccacgcag cataaacacc cccgccataa ccatacaaat cactgcctgc
     4921 cgcaccgcct ttgccgttga ctgatgcgtg ctggccggcc ctgccgccca tcacgccgat
     4981 tgccagtcgg ctgccttcat tttgccgtac aaacacctcg ccgccgattt gcacgccttt
     5041 gcgccacccg tccgcagccg cgccgccccg tatattttga tgcgaccggc cgccgatgaa
     5101 gcgcagccac aatttttgac gggacggatc ggcggcatac acgtcgtcgc ccctgtcggc
     5161 ggcacgcagc ccaaacaaag tatttgccgc ataagcctgt tgggcatata aaaccgcttc
     5221 cgcctcgggg atagccaaac gctgcacgcc caagcaaaat ccgccttcgc attgcttgag
     5281 caataattcg tacagtccct gccggtaatt ttgtttaaac gcaaacgaac cggaatccgc
     5341 cgtttttgcc gcaatcagtt tgcgcccgtc gagcgttttc agattttccg gccggcgttc
     5401 caaaaccacg ccggattgtc cgctgacttt gtcttcaaag gtcagggcgg gttcgtccga
     5461 ctcccgcacg tcgtagccga acaggaaatc cgcgccgccc gcataatcct tatggacggt
     5521 cagtttttca tctttcgtgt tcaggcggat gcgggcatta tcggaagttt ccagcgtatg
     5581 gatgtcggaa tggcggcgcg gttcccaaag ggaatctttc agcgtcatct tggtcagttc
     5641 caacacctgt ccggcgacgt gggatttgtt ttcgattttg acgtgtaatt ttttattgaa
     5701 gatgttgttg agactagtct gtgatgtatt actatgagga gtgagtacgg aatcttctac
     5761 caatttatta attaaatcgg gcatcgtctg tttgagcgta ctaaattttg ttccaaactg
     5821 tgctatatac gcctcctcag tccgtttttt attttctgcc caagcttccg gtcttgtttt
     5881 gtataaatcc tgtaattgac ttttaaccgc ctgctttaaa tcagccccat acttctctgt
     5941 aatatatttt ttgacaacgg gcatattatc gacggcgagg atgccgctat tgtaattttt
     6001 accttggata ttgatttcgt catacccata ttcggatgaa ttttgatcgg ttttaccgct
     6061 ggaaaaataa tgggaagtgc cgggtttcgg cttgtctgaa aatgaaaaat cggcatcgtc
     6121 gccgattttg gtagaacgag tatttgaacc aagctgagct ctgaacatac cgaagcaatc
     6181 aaaagatatg ctgcacgggc cgcgccaaac gtgggaattt ctgtcttccg ccgcttctaa
     6241 aataaatttg tttttaatct cggcttcttt gacgggatcg tacgcatccg aacctcaaac
     6301 atatggcgtg caaaacagcg ataaaacaga taatgttaat agattaaccc gaccgcttgc
     6361 ttgcttgctt gcttgcttgc ttgcttgctt tcagtgtcat gagaaatttt cctttgtcaa
     6421 gtgttaaaat tataatgatt atatactaca ttatactaca tcgcaatgaa aaatcaggaa
     6481 aagcaaaaaa ccctccgccg tcattcccgc gaaagcggga atctagaatt ttaatgccgc
     6541 aagaatttat caaggatggt cggggtttaa aggtctggat tcccgcctgc gcgggaatga
     6601 cggtttagaa atcacccgaa acaagaaaaa gaaccgaaac cgaacaagcc ggattcccgc
     6661 ctgcgcggga atgacgggat ttcaggttac tgtttttgat tttctgtttt gtggaaataa
     6721 cggga
//
LOCUS       DAWGRZ010000117         6609 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000117 DAWGRZ010000000
VERSION     DAWGRZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.117"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            353..2134
                     /locus_tag="WHT44_001539"
     CDS             353..2134
                     /locus_tag="WHT44_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ2533706.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHSVLFADYGFGLPLTGVESERGGNLALHWFANCADIDAESWLARHSDGLPAGIST
                     PQPSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDAEGQSAWTLCFSPELFLKIGSDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGTVCVPEPFKVSRFGSVWQMTSTIQAQALPHTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLEAEARGLYTGSIGYLNPCSGGLGFEGTFNV
                     VIRTLSLTPLSDGIYQGVYGVGSGIVIDSDPAAEYRECGWKARFLNELRPDFGIFETL
                     RAENGRCTLLDRHLCRLKTSAQALNLPLPDGCENQIKQYIADLPDGAFRVKALLASDG
                     ISLSRAVLNRLTDKQRVIISPAVLPAQNYLRRFKTTCRALFDQALQTAETQGAFDSLF
                     FNSDGILLEGGRSNVFIKHRGQWLTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETH
                     ITQKTLQEAEEIRLSNALRGVFAAALA"
     gene            complement(2193..3710)
                     /locus_tag="WHT44_001540"
     CDS             complement(2193..3710)
                     /locus_tag="WHT44_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ2533707.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTPLADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGIAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(4406..6040)
                     /gene="groL"
                     /locus_tag="WHT44_001541"
     CDS             complement(4406..6040)
                     /gene="groL"
                     /locus_tag="WHT44_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ2533708.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEDKPAVPDMGGMGGMGGMM"
     gene            complement(6133..6423)
                     /gene="groES"
                     /locus_tag="WHT44_001542"
     CDS             complement(6133..6423)
                     /gene="groES"
                     /locus_tag="WHT44_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ2533709.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGSRRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
BASE COUNT         1499 a         1999 c         1564 g         1547 t
ORIGIN      
        1 cgcagacagt acagatagta cgaaaccgat tcactcggtg cttcagcacc ttagagaatc
       61 gttctcttcg agctaaggcg aggcaccgct gtactggttt ttgttaatcc actatacttg
      121 gagctggtct tgcttttcgc ctaattctac gttttcaaac ggttgcagct ggtggtctgc
      181 cataaaggtc tccttattgt atttcaggtt ggaaatcgga atttgttttc acaattttac
      241 accttcgccc ccgctttctc tacataaaat tacattttgc cgatatttgc cgaattgtct
      301 gaaaatatgt gtaataaggg gcgtataatc aaaacatttg ccccggattg ccatgcctta
      361 tttcgccctg tttgacgatg ccgtaagcgg ccgcgcaaaa cgctatcaaa atcatgtgga
      421 aagccgtttt ttccgtcccg aagaactcga tgctttggac ggcgcgctgc aatcgggctg
      481 gcaaaaaggg ctgcattcgg tgttgtttgc agactacgga ttcggtttgc cgctgacggg
      541 ggttgagtcc gaacgcggcg gcaatcttgc cctgcactgg tttgccaact gcgccgacat
      601 cgatgccgaa agctggcttg cccgacactc agacggcctc cccgccggca tttccacgcc
      661 gcaaccctcc gtatccgaaa ccgattacct cgaccgcatc cgccaaatcc acgaagccat
      721 ccggcgcggc gacacctatc aaatcaacta caccacccgc ctgcacctgc aagcctacgg
      781 caatcccgtc agcctctacc gccgcctgcg ccagcccgtc ccctatgccg tcttgtccca
      841 cctgcccgat gcggaggggc aatccgcgtg gacgctgtgt ttctcgcccg aactcttcct
      901 caaaatcggt tcggacggca ccatcagcac cgaaccgatg aaaggcaccg cgccgatttt
      961 gggcgacgga caagacgaac gccgcgccgc cgagttgcaa gcagacccga aaaaccgcgc
     1021 cgaaaacgtg atgattgtcg atttgctgcg taacgatctc ggcaaaatcg cccaaaccgg
     1081 cacagtatgc gtacccgaac cgtttaaagt atcgcgtttc ggcagcgttt ggcagatgac
     1141 cagcaccatc caagcccaag ccttgccgca cacctcgttc gccgacatcc tccgcgccgc
     1201 cttcccctgc ggcagcatca ccggcgcgcc caaaaaaatg agtatgcaga ttatcgaatc
     1261 gctcgaagcc gaagcgcgcg gactttatac gggcagcatc ggctatttga acccgtgttc
     1321 cggcggcttg gggtttgaag gcacgttcaa cgtcgttatc cgcaccttgt cgctcacgcc
     1381 gctttcagac ggcatttatc aaggcgtgta cggtgtcggt tccggcatcg tcatcgacag
     1441 cgaccccgcc gccgaatatc gcgaatgcgg ctggaaagcc cgtttcctca acgaattgcg
     1501 ccccgacttc ggcatttttg aaaccctgcg cgcggaaaac ggacgctgca ccctgctcga
     1561 ccgccaccta tgccgtctga aaacctccgc ccaagccctc aacctgcccc tgcccgacgg
     1621 ctgcgaaaat caaatcaaac aatacattgc cgacttgccc gatggcgcgt tccgcgtcaa
     1681 agccctgctc gcttcagacg gcatcagcct gtcccgcgcc gttttaaacc gtctgaccga
     1741 caaacaacgc gtcatcattt cgcccgccgt cctgcccgca caaaactacc tgcgccgctt
     1801 caaaaccacc tgccgcgccc tcttcgacca agcgttgcaa accgccgaaa cacaaggcgc
     1861 gttcgacagc ctgtttttca attcagacgg catcctgctc gaaggcggca gaagcaacgt
     1921 cttcatcaaa catcgcggac aatggctcac accctcttta gatttagaca ttttaaacgg
     1981 cataatgcgc caagccgtat tggacgaacc gcaaaaatat ttgcaaacaa atcaagtaat
     2041 cgaaacacac atcacacaaa aaacactgca agaagccgaa gaaatccgcc tctccaacgc
     2101 cttgcgcggc gtatttgccg ccgcccttgc ctgaacgcgc aaaaatgccg tccgaacctg
     2161 tttccaaagt tcggacggca ttatcccacc attcaaaacc gccaatccgc cgacacaaac
     2221 acctcgctgt tgcggcgttt cgcatacggc acattacttt ccgtcctgcc gaaacgataa
     2281 ttcaacgccg gcacgatacc tttgtacgac aacttgtcgt ggctcaaagc cagcgagaca
     2341 ttccattcgc ggttgcgttg cgcctctgtc gagaaagccg caatgccctt atagttgcgg
     2401 cgggcataag acgcggaaac ccgactgttc aaaccgccca actgccgcca ctcctgcgcc
     2461 caaccggcat aaacaccgtt gcgccggtag gcggcattat tgaccgcgcc gcccaccgtt
     2521 tcgcgtttcg gcacaaaccg cacaaactgc cagccgccga acacagttgc cgattcgccc
     2581 aaacgttttg ccgacgaaac ataaaacccg tcctgcctgc cgttattgta ttccgcccta
     2641 tcctgttcgc ggtagcgttg gcggtaatgt tccagcgcga ccgaaaattg ccatcccggg
     2701 tttgggcggt aagtatggga cagctgcacg ccgactccgt gcgccagcat atacggcggc
     2761 aggcggcggt tgtttacccg ttttgttttc gcatcaaagc cgtcgctgcc cgacaactgc
     2821 acctgataaa acggcaaaat ccccgccgtc tgccgtgcat ttttatactg ccaacccaaa
     2881 tacgccctgc cgaacccgtc atcataagct gattttttac tgaaataata gctcgtgccg
     2941 ccgatattgg aacggaacaa caaataatga ttatctgcca acggcgtcag cttttccgcc
     3001 tcgatttcat aattcaaccc tgccgcccgc tccgcccggc tgacactgca tatctgccgg
     3061 cctccgtttt gccggcaata ttgcggcgcg gcattattgg catttctatt gaccgccgga
     3121 ctgatgccgc ccgaaaaacg ccagcccgtc agcccctccg tttttttccg aaaacgcccc
     3181 acattttcca aaaccggtgc cggcaaatcc aattttgccg cctccgcaaa atgcctttct
     3241 gccgacttca gccggaaatc gtcaaactcc gccgccgcca aatccagcaa aatccgctcg
     3301 tctgccgcat tttccccgtg cagttcccga taccgcgcca ccgcctccgc cggccttccc
     3361 gccaatttcg ccagcaaagc ccgcgccctg ccgtacaaaa ccgcgtcata atccggcagc
     3421 ttggcataca aatccgccaa cgaagcgatt aaatccgcct gattgccgtt gagcgcgtcg
     3481 cgcaaactat gttccaacat tttcggatgc gccaacaaaa aatccccgtc aaccacgcgc
     3541 ggggcatcat tttcaacttt ccaatctgat tccgcccact tatccgacac cgaccgctgc
     3601 acctgcaaca atgccttgtc atccaaaatc gcgggcgcat ccgccccata ggcggcagaa
     3661 acacctgccg cacaccaaac aaccaaaaag ccgtatctga aatacaacat accctgtcat
     3721 ttacctttct ggcaaacacg ccgccgaagc acgtcaaacc atccgaaaaa caggcagaaa
     3781 cccgtgaaaa ccggctttgc cgcctgaaag caggcaaaca aaaaccgccg cccgattttc
     3841 aaagggcgga tttcacattt atagtggatt aacaaaaatc aggacaaggc gacgaagccg
     3901 cagacagtac aaatagtacg gaaccgattc actcgtgctt aagcacctta gagaatcgtt
     3961 ctctttgagc tgaggcgagg caacgccgta ctggtttttg ttaatccact ataacagcaa
     4021 ccctgtcgcc gtcattcccg caaaagcggg aatgacgaag ctatccgcac agaaacctgc
     4081 accacgtcat tcccacgaaa gtgggaatcc agaacgtaaa atctgaagaa accgttttat
     4141 ccgatacgtt tccgcaccga cagacctaga ttcccgcttt cgcgggaatg acggcggaaa
     4201 ggttgctgtt tttccgataa attcctgccg ctcttcgttt ttgggatggc gggaaataaa
     4261 acaaaagcgc gcgtatcaaa aaacaaaaat gcaaagaacg gcgttggcaa ttttaaggtt
     4321 tgcgccttag ccgcacttat tcgcaaaaaa aaccgcacgg cgttgaccgt gcggtttttt
     4381 atctgaaagc ttcagacggc attgcttaca tcatgccgcc cataccaccc atgccgccca
     4441 tatcaggcac agccggtttg tcttcgggga tttcagcgat catgcaatca gtggtcagca
     4501 tcaagccggc gatagatgcg gcgtgttgca gcgcagaacg ggttactttg gcggggtcga
     4561 gtacgcccat ttcgatcata tcgccgtatt cgccgctgcc agcgttgtaa ccgtagttgc
     4621 ctttgccttc caatactttg ttcacaacca cgctgggttc gccgcctgcg ttggcaacga
     4681 tttggcgcag cggagactca acggcgcgca agacgatttg tacgcctgcg tcttggtcgg
     4741 cattgccggt gtgcaggttt tccaaagcag cacgggcacg caacagggct acgccgccgc
     4801 ctgcaaccac gccttcttca acggctgcgc gggtagcgtg cagcgcgtct tccacgcggt
     4861 cttttttctc tttcatttcg acttcggtcg cggcaccgac tttgatgact gccacgccgc
     4921 ctgccaattt agccacgcgc tcttgcagtt tttctttgtc gtaatcgctg gttgcggttt
     4981 cgatttgttg gcggatttcg gcaacacgcg cttcgatttg ggctgcgtcg ccaaagccgt
     5041 cgatgatggt ggtgttttct ttaccgattt cgatgcgttt ggcttgaccc aagtcgtcca
     5101 aagtcgcttt ttccaaagac agaccgactt cttcggaaat caccacgccg ccggtcagga
     5161 tggcgatgtc ttgcaacatc gctttgcggc ggtcgccgaa gccaggggct ttgacggcaa
     5221 cggttttcag gatgcctcgg atgttgttca cgaccaaagt cgccaaggct tcgccttcta
     5281 cgtcttcagc gataatcaac agcggacggc tggcttttgc cacttgttcc aaaacaggca
     5341 gcaggtcgcg gatgttgctg atttttttgt cgaacaacaa tacaaacgga ttgtccaaag
     5401 cagcgatttg tttttccgca tcgttgatga agtaaggaga caggtagccg cggtcgaact
     5461 gcataccttc aactacgtcc agctcgtttt ccaaagactt gccgtcttca acggtaatca
     5521 cgccttcttt gccgactttt tccatcgctt cggcgataat cgcgccgact tgttcgtcgg
     5581 agttggcgga aatagagccg acttgggcga tttctttaga agtgtcgcaa ggtttggcga
     5641 tgtttttcag ttcgtcaacc aaagcggcga cggctttatc gataccgcgt ttcaggtcgg
     5701 tcggattcat acctgcggta acatatttca taccttcggc aacgatggat tgcgccagta
     5761 cggtggcggt agtcgtaccg tcgcctgcca cgtcgttggt tttggacgca acttctttca
     5821 ccatttgcgc gcccatattt tcaaacttgt ctttcagttc gatttctttg gcgacggtta
     5881 cgccgtcttt ggtgatgtgc gggccgccga atgcgcggtc aacgactacg ttgcgacctt
     5941 tggggcccaa ggttacgcgg acggcgtttg ccagaatgtt cacgccgttt accatttttt
     6001 gacggacttc attgccgaac tgtacgtctt ttgctgccat ttcaattctc caaaaatcat
     6061 taaaactgtc tgataaaacc gtttatgccg tctgaaggcg gtttgccgtt tcagacggca
     6121 tcgtgtccgt atttattttt caacgatgcc gaaaatatct tcttcgcgca ttaccaacag
     6181 ctcttcgccg tcggctttta cggtttggcc gctgtatttg ccgaagatga ttttgtcgcc
     6241 gactttgaca tccagcggac ggcggctgcc gtctttaccg attttgcccg cgcccacggc
     6301 gatgacttcg cccatatcgg gtttttcggc ggccgcaccc ggcaaaacga tgcccgatgc
     6361 ggttttttct tcagcttcca agcgtttgac gacaacgcgg tcgtgtaaag gacggatggt
     6421 catatttatg ctccgataaa atagtttgaa aacaatcatc tgcccgaacg gttcaggcag
     6481 attgaagtgg aaaccggaca gccgtcaagc agctgcccgt ataagtcggc aaaattaggg
     6541 tgtgtgcggg taaattcaag tgaggcggaa aaaatttatt tccgccgttt tttatagtgg
     6601 actaaattt
//
LOCUS       DAWGRZ010000118         6465 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000118 DAWGRZ010000000
VERSION     DAWGRZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.118"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(90..1886)
                     /locus_tag="WHT44_001543"
     CDS             complement(90..1886)
                     /locus_tag="WHT44_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ2533710.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLAGSGIVLQKSGQVPPYNEWLAAS
                     LPENAAVGIPSDMVSLTGKRTLAQSLAAKNIRIEHPDNLLNQVWTNRPALPAETVFIH
                     DPDYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGIAVEPYAQVADKLAQIGGVLLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPSAEQKSDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            1942..2283
                     /locus_tag="WHT44_001544"
     CDS             1942..2283
                     /locus_tag="WHT44_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533711.1"
                     /translation="MPSEKHNHLIKSPACCKTDGLIGWCVFARKKRCKVVCCKILGIK
                     CRFMPFFGSNMENIVKFEHEVLIVCVGRYLDYVTIRVFIKISLPGGILFLKTVGFGFV
                     WGQSRPNWSNR"
     gene            2344..3597
                     /locus_tag="WHT44_001545"
     CDS             2344..3597
                     /locus_tag="WHT44_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ2533712.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQSDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3752..5263
                     /gene="lysS"
                     /locus_tag="WHT44_001546"
     CDS             3752..5263
                     /gene="lysS"
                     /locus_tag="WHT44_001546"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ2533713.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVVQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(5340..5528)
                     /locus_tag="WHT44_001547"
     CDS             complement(5340..5528)
                     /locus_tag="WHT44_001547"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ2533714.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAK
                     SEQRLTALSEWVGQLETE"
     gene            complement(5532..6409)
                     /locus_tag="WHT44_001548"
                     /pseudo
     CDS             complement(5532..6409)
                     /locus_tag="WHT44_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
BASE COUNT         1403 a         1671 c         1853 g         1538 t
ORIGIN      
        1 cttgcggcaa gatgaaaacg gtgcgggatg ttttgggaaa ccaatgccgt ctgaagggct
       61 ttcagacggc attttttgcg ccgtgctgtt taacgcgcca gcggttcggt gcgtttgatc
      121 agccacgctt ttgccgcgcc ttcggtcagc ggctcgaggc ggcggcggac ttcggcgtgg
      181 tagcggttga cccagtcgat ttcgccgtcg gtcatgaggg cggtgtccat caggcgggtg
      241 tcgatggggc agagggtcag ggtttcaaaa cagaggaagc tgccgaattc ggtttcttga
      301 ggggcggcga cggcttggtt ggcggcaagg ttttcaatgc ggatgcccca ttttcccggg
      361 cggtagagtc cgggttcgat ggaggtaacc atgccttttt tcatggcggt ttcgggcgtg
      421 gcgggggcgg cgaaggcgat gcgctgcggg ccttcgtgga cgttgaggaa atagcctacg
      481 ccgtgtccgg tgccgtggcc gtagtcgcat tgcgcctgcc acaggggttt gcggcaaatc
      541 gcatcaatca gcggcgaggg gatgttttcg gggaacacgg cttcggcaag cgcgatatgg
      601 gctttgagaa cgagggtgtt gtcgcttttt tgttcggcac tcggcgtgcc gacggggacg
      661 acgcgggtga tgtcggtcgt gccgcctttg tattgcgcgc cggagtcgat gagcaaaagc
      721 ccgttgccgc tgatggtgct gtggctttcg ggtgtcgcgc tgtaatgcgg cagtgcgccg
      781 ttggcgttga agcctgcgat ggtgtcgaaa ctcaatgaaa tgaagcctgg gcgcacgctg
      841 cggtggcgat aaagcatggt gtccacgtcg atttcggtca ggctgccgcc gttgccgatg
      901 atgtcttcaa actcggcgaa gaaaccgcac aacgccgcgc cgtcgtgttc catcgcttcg
      961 cggatgcggg cgatgtcggc ttcggatttg caggatttga acagcgtgga tgggttgatt
     1021 ccctcgataa ggcgcacgct ttcgggcagg cgcacaagcg tgctgacggc ggttttgttc
     1081 ggctcgatga gcagcacgcc gccgatttgc gcgagtttgt cggcaacttg ggcgtaaggt
     1141 tcgaccgcga tgccggcggt ttgcagcgcg gcggcggctt cggcgttcag acggcatcgg
     1201 tcggtaaaca ggacggcgtt gtctttgcca atcagcagga aggacacgaa aacgggattg
     1261 aaaggcacgt cgctgccgcg caggttggtc agccaggcga tgtcgtcaag cgaggaaacc
     1321 aagtggtaat ccgcgccttt ttccgccatc acggcgcgca cgcgggcgag tttttcggcg
     1381 gcggtttcag aaacatagtc ggggtcgtgg atgaacaccg tttcggcggg gagggcgggg
     1441 cggtttgtcc acacttgatt cagtaaatta tccgggtgtt cgatgcggat gtttttggcg
     1501 gcgagtgatt gcgccaaagt gcgtttgccg gtgagcgaga ccatatcgga agggatgccg
     1561 acggcggcgt tttcgggcag gcttgccgcg agccattcgt tgtacggcgg cacttgcccg
     1621 cttttttgca gcacaatgcc gctgcccgca agctgtttgg cggcttgttc ccaatagcgg
     1681 ctgtccaccc acacgcccgc ttcatcggtg gtcaggacaa acgtgccgac cgagccggta
     1741 aagcccgata attcgcggcg cgcctgccaa tgctcgggca ggtattcgga caggtggggg
     1801 tcggcggaag ggatgacgag tgcatccaag ccttgcgcct tcatggcttc gcgtaatgcg
     1861 gacagataat tcgatacggt attcataaca gttcctccaa tcgggttttg cggcttcaga
     1921 tggcatggac ggaaatctga aatgccgtct gaaaaacata accatcttat caaatcgcca
     1981 gcctgctgca aaacggacgg gctgatcgga tggtgcgtgt ttgcgcgaaa aaagcgatgt
     2041 aaggttgttt gttgtaaaat tttaggcata aaatgtcgtt ttatgccttt ttttggaagt
     2101 aatatggaaa atatagtcaa gtttgagcat gaagttctaa tagtgtgtgt tggcaggtat
     2161 ttggattacg ttacaatacg tgttttcatc aaaatttccc taccgggcgg tatcctgttt
     2221 ttgaagacgg taggatttgg gtttgtttgg gggcaatccc gcccaaattg gagcaataga
     2281 tagggctgtg tccgaatatg cggctctgtg tgttgaaaca cagttaaaaa tacggaagac
     2341 tttatgtccg aacaacatat ttcgacttgg aaaagtaaaa tcaacgcatt gggtccgggg
     2401 atcatgatgg cttcggcggc ggtcggcggt tcgcacctga ttgcctcgac gcaggcgggc
     2461 gcgctttacg gctggcagat cgcgctcatc atcatcctga ccaacctctt caaatacccg
     2521 tttttccgct tcagcgcgca ttacacgctg gacacgggca agagcctgat tgaaggttat
     2581 gccgagaaaa gccgcgttta tttgtgggta ttcctgattt tgtgcatcct ctccgccacg
     2641 attaacgcgg gcgcggtcgc cattgtaacc gccgccatcg tcaaaatggc gattccctcg
     2701 ctgatgtttg atgccggcac ggttgccgcc ttgattatgg catcctgcct gattattttg
     2761 gtgagcggac gttaccgcgc tttggatcgc gtttccaaaa tcatcatcgt tactttgagt
     2821 atcgccacgc ttgccgccgc cggcatcgct atgtcgcgcg gtatgcagat gcagtccgat
     2881 tttatcgagc cgacaccgtg gacgcttgcc ggtttgggct tcctgatcgc gctgatgggc
     2941 tggatgcccg cgccgattga aatttccgcc atcaattctt tgtgggtaac cgaaaaacaa
     3001 cgcatcaatc cttccgaata ccgcgacggg atttttgatt tcaacgtcgg ttatatcgcc
     3061 agtgcggttt tggctttggt tttccttgca ctgggcgcgt ttgtgcaata cggcaacggc
     3121 gaagcagtgc agatggcggg cggcaaatat atcgggcaat tgatcaatat gtacgccgtt
     3181 accatcggcg gctggtcgcg cccgctggtg gcgtttatcg cgtttgcctg tatgtacggc
     3241 acgacgatta ccgtcgtgga cggctatgcc cgtgccattg ccgaacccgt gcgcctgctg
     3301 cgcggaaaag acaaaacggg caacgccgaa ttctttgcct ggaatatttg ggtggcgggc
     3361 agcggtttgg cggtgatttt ctggtttgac ggcgtaatgg cgaatctgct caaatttgcg
     3421 atgattgccg cttttgtgtc cgcccctgtg tttgcctggc tgaattaccg tttggttaaa
     3481 ggtgatgaaa aacacaaact cacatcaggt atgaatgccc ttgcattggc aggcttgatt
     3541 tatctgaccg gttttaccgt tttgttctta ttgaatttgg cgggaatgtt caaatgataa
     3601 cgatgccgtc tgaaaccgca aaccgctttc agacggcatt gtcgcgttta tggaaaaaaa
     3661 tgccgacatc gcgtaaaata tgcgcaaatt ttgtgattcg gtcaggtcgt ctgaaacaga
     3721 ttgcgcctga tttatttttt cggaaaacct tatgagcgaa caaaaccatc cgcaaaccga
     3781 gccgcagttg gacgaaaacc aaatcatcgc cctgcgccgc gaaaaactgc acaacatccg
     3841 ccaacagcgc aacgcctatc ccaacgactt caaacgcgac agcttcgccg ccgatttgca
     3901 cgcccaatac ggcgaaatcg gcaaagaaga actcgatccg caaggcattc ccgtcaaagt
     3961 ggccggccgc atgatgctga agcgtcaaat gggcaaggcg agttttgcca ccattcaaga
     4021 cgtgtccggg caaatccagc tttatctgaa caacaaaggc gtgagccaag aagttttgga
     4081 cgacttcaac cattgggatt tgggcgacat cgtcggcgcg gaaggcactt tgttcaaaac
     4141 caaccacggc gaactgaccg tacgcgtgtc cggcatccgc ctgctgtcca aatccctacg
     4201 cccgctgccc gacaaacaca aaggtttgag cgatcaggaa accaaatacc gccaacgcta
     4261 tgttgatttg attgccaatg aagaatcgcg caataccttt atcaaacgca gccaaatcat
     4321 ccaatccgtg cgtaatttta tggtgggcga gcattatctc gaagtcgaaa ccccgatgat
     4381 gcacccgatt cccggcggcg cgacggcaaa acccttcgtt acccatcaca atgccttaga
     4441 tattccgctt tacctgcgta tcgcgcctga gctgtatttg aaacgcctgg ttgtcggtgg
     4501 tttggaacgc gtgtttgaaa tcaaccgcag cttccgcaac gaaggcatgt ccgtgcgcca
     4561 caaccccgaa ttcaccatga tcgaattcta cgaagccttc tccgactacg aacgcatgat
     4621 gcagatggcg gaagacatca tccgcaacgc atcgcgcacg gtaaacggca cggcaaacat
     4681 cacttacaac ggcaaagaag tcgatttgga aagcccgttt gaacgcctga ccattctcga
     4741 agccatcaaa aaatacaatc cgcactacac cgacgagcag ttgaacgatg cggaatggct
     4801 gaaaaaagaa atcgtcaaac acggcgaaag cctgccgccg tccccgggca tcggcagcct
     4861 gcaactcgcg ctgtttgaag gttgcgccga gggcaagctg tggaatccga ccttcatcgt
     4921 cgattacccg gtcgaagttt caccgttggc gcgcgcttcg gataccaaac aaggtctgac
     4981 cgaacgtttc gaattgttcg ttgtcggccg cgaactggca aacggctatt ccgagttgaa
     5041 cgaccccgaa gaccaagccg aacgcttcaa agcgcaagtg gtgcaaaaag acgcgggcga
     5101 cgacgaagcc atgcactacg atgccgacta catccgcgcg atggaattcg gtttgccgcc
     5161 gaccggcggt tgcggcatcg gtatcgaccg cttggtaatg ctgctgaccg attcgcaaac
     5221 catccgcgat gtgattctgt ttccgcagat gcgccccgag taatcataaa aacagttgaa
     5281 atggcaatgc cgtctgaacc cgattggatt cagacggcat tttgtatggc ggtacggatt
     5341 tattcggttt ccaactgacc gacccattcg gacaaggcag tcaggcgttg ttcggatttc
     5401 gccacgaacg ggttttcggt atcccacgcg tagcctgcca aaatcgaaga aagcatacgg
     5461 ctgatttccg ggctgcggtc gggcgcgtac acgacgtttt ggaagcggaa atcataccaa
     5521 ccgtccacac atcaggggcg gtaatccgaa tcaacacgcc ttcttgcctg tgcggcggac
     5581 ataaaccgtt ttttgactcc agcaccacac ctaaaatcgt gctgccttcg ggcaggtcaa
     5641 aggcttcaga cggcctgccc gtctgataaa tcagcatcct gcccgcaata tcgataacaa
     5701 ggtgcgcgac gaccagccgc ttgtcggaca gttcaatcag cgcgatgtct tcgtccgcag
     5761 ggcacatacg cggctcgacc accagcaagt ctcccttttc gataaccggc gacatggacg
     5821 tgtcgggcat ggcaacggca aacgtgtact gcgagggggc gaccaccgaa gcaaacaggt
     5881 cgaaactgcc cgtgagtttg cctgtacggg cgaaagccgc cgcctgacgg tctgacaaca
     5941 aaggacgggg tgtgagcctc atcgacagtg ctttgccatc ggcatcgctg tggttgtgcg
     6001 tatgcgtatg atggttgttg atggtcgtat cgccttggac atcgttaagg actatgctgt
     6061 ttgaacctgc cacggatttg gtcatctcat tgtaggcttg cgtcgtgatg cgctcttgct
     6121 tggtcatacc tgttacagcc tgtttcagca tttctaacgc tatgtgattc ttcctaaggg
     6181 tcgctatacc ggcttttgca gcttgctcat taccacaaga ggggcaaccc cagctacttc
     6241 ctatgaacat attggagtac ctcgaacagg tttggtttcc atgtttaggg cagttgatta
     6301 cacacggctt ggctgtaccc tcaaactcga ccaacttaat gccacgatga ccgaaacgtt
     6361 cctcaacctc agcagcagct tcttcaaatg tttttgacct tcttgccata ttgacctccg
     6421 gattattgat tattttggct atgctttaac gagggattgg aatga
//
LOCUS       DAWGRZ010000119         6457 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000119 DAWGRZ010000000
VERSION     DAWGRZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.119"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            284..2131
                     /locus_tag="WHT44_001549"
     CDS             284..2131
                     /locus_tag="WHT44_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ2533715.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVLIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKAYPLAKLGNTQAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGMPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGVWYVR
                     DRAGIDARLGAEFLAEGRKIPGSAVDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            2186..6355
                     /locus_tag="WHT44_001550"
     CDS             2186..6355
                     /locus_tag="WHT44_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ2533716.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGAAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIANAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNGKGK"
BASE COUNT         1509 a         2074 c         1704 g         1170 t
ORIGIN      
        1 ttgcgttgat ttgccctatg cagtagtgcc ggacaggctt tgctttatca ttcggcgcga
       61 cggtttaatt tattgaacga aaataaattt atttaatcct gcctattttc cgacactatt
      121 ccgaaacgca gcctgttttc catatgcgga ttagaaacaa aataccttaa aacaagcaga
      181 tacatttccg gcgggccgca acctccgaaa taccggcggc agtatgccgt ctgaagcgtc
      241 ccgccccgtc cgaacagtgt taaaatcgaa agccgccaca ccgatgcacg acacccgtac
      301 catgatgatc aaaccgaccg ccctgctcct gccggcttta tttttctttc cgcacgcata
      361 cgcgcctgcc gccgaccttt ccgaaaacaa ggcggcgggt ttcgcattgt tcaaaaacaa
      421 aagccccgac accgaatcag tcaaattaaa acccaaattc cccgtcctca tcgacacgca
      481 ggacagtgaa atcaaagata tggtcgaaga acacctgccg ctcatcacgc agcagcagga
      541 agaagtattg gacaaggaac agacgggctt cctcgccgaa gaagcgccgg acaacgttaa
      601 aacgatgctc cgcagcaaag gctatttcag cagcaaagtc agcctgacgg aaaaagacgg
      661 agcttatacg gtacacatca caccgggccc gcgcaccaaa atcgccaacg tcggcgtcgc
      721 catcctcggc gacatccttt cagacggcaa cctcgccgaa tactaccgca acgcgctgga
      781 aaactggcag cagccggtag gcagcgattt cgatcaggac agttgggaaa acagcaaaac
      841 ttccgtcctc ggcgcggtaa cgcgcaaagc ctacccgctt gccaagctcg gcaatacgca
      901 ggcggccgtc aaccccgata ccgccaccgc cgatttgaac gtcgtcgtgg acagcggccg
      961 ccccatcgcc ttcggcgact ttgaaatcac cggcacacag cgttaccccg aacaaatcgt
     1021 ctccggcctt gcgcgtttcc agcccggtat gccgtacgac ctcgacctgc tgctcgactt
     1081 ccaacaggcg ctcgaacaaa acgggcatta ttccggcgcg tccgtacaag ccgacttcga
     1141 ccgcctccaa ggcgaccgcg tccccgtcaa agtcagcgta accgaggtca aacgccacaa
     1201 actcgaaacc ggcatccgcc tcgattcgga atacggtttg ggcggcaaaa tcgcctacga
     1261 ctattacaac ctcttcaaca aaggctatat cggttcggtc gtctgggata tggacaaata
     1321 cgaaaccacg cttgccgccg gcatcagcca gccgcgcaac tatcggggca actactggac
     1381 aagcaacgtt tcctacaacc gttcgaccac ccaaaacctc gaaaaacgcg ccttctccgg
     1441 cggcgtctgg tatgtgcgcg accgcgcggg catcgatgcc aggctggggg cggaatttct
     1501 cgcagaaggc cggaaaatcc ccggctcggc tgtcgatttg ggcaacagcc acgccacgat
     1561 gctgaccgcc tcttggaaac gccagctgct caacaacgtg ctgcatcccg aaaacggcca
     1621 ttacctcgac ggcaaaatcg gtacgacttt gggcacattc ctgtcctcca ccgcgctgat
     1681 ccgcacctct gcccgtgcag gttatttctt cacgcccgaa aacaaaaaac tcggcacgtt
     1741 catcatacgc ggacaagcgg gttacaccgt tgcccgcgac aatgccgacg ttccttcagg
     1801 gctgatgttc cgcagcggcg gcgcgtcttc cgtgcgcggt tacgaactcg acagcatcgg
     1861 acttgccggc ccgaacggat cggtcctgcc cgaacgcgcc ctcctggtgg gcagcctgga
     1921 ataccaactg ccgtttacgc gcaccctttc cggcgcggtg ttccacgata tgggcgatgc
     1981 cgccgccaat ttcaaacgta tgaagctgaa acacggttcg ggactgggcg tgcgctggtt
     2041 cagcccgctt gcgccgtttt ccttcgacat cgcctacggg cacagcgata agaaaatccg
     2101 ctggcacatc agcttgggaa cgcgcttcta aaccgatatg gccacttcag acggcattgc
     2161 agcaaaccat tttgaaacag acattatgac cgataccgca ccgacagata ccgatccgac
     2221 cgaaaacggc acgcgcaaaa tgccgtctga acaccgccct accccgccgg caaaaaaacg
     2281 ccgcccgttg ctgaagctgt cggcggcact gctgtctgtc ctgattttgg cagtatgttt
     2341 cctcggctgg ctcgccggta cggaagcagg tttgcgcttc gggctgtacc aaatcccgtc
     2401 ttggttcggc gtaaacattt cctcccaaaa cctcaaaggc acgctgctcg acggcttcga
     2461 cggcgacaac tggtcgatag aaaccgaggg ggcagacctt aaaatcagcc gcttccgctt
     2521 cgcgtggaaa ccgtccgaac tgatgcgccg cagcctgcac attaccgaaa tttccgccgg
     2581 cgacatcgcc atcgttacca aaccgactcc gcctaaagaa gaacgcccgc cgctcagcct
     2641 tcccgacagc atagacctgc ctgccgccgt ctatctcgac cgcttcgaga cgggcaaaat
     2701 cagcatgggc aaagcctttg acaaacaaac cgtctatctc gaacggctgg atgcttcata
     2761 ccgttacgac cgcaaaggac accgccttga cctgaaggcc gccgacacgc cgtggagcag
     2821 ttcgtcgggg gcggcctcgg tcggcttgaa aaaaccgttt gccctcgata ccgccattta
     2881 caccaaaggc ggactcgaag gcaaaaccat acacagtacg gctcggctga gcggcagcct
     2941 gaaggatgtg cgcgccgaac tggcgatcga cggcggcaat atccgcctct cgggaaaatc
     3001 cgtcatccac ccgtttgccg aatcattgga taaaacattg gaagaagtac tggtcaaagg
     3061 gttcaacatc aatccggccg ccttcgtgcc ttccctgccc gatgccggac tgaatttcga
     3121 cctgaccgcc atcccgtcgt tttcagacgg catcgcgctg gaaggttcgc tcgatttgga
     3181 aaacaccaaa gccggctttg ccgaccgcaa cggcatcccc gtccgtcagg ttttaggcgg
     3241 ctttgtcatc cggcaggacg gcacggtgca tatcggcaat acgtccgccg ccctgctcgg
     3301 acggggcggc atcaggctgt cgggcaaaat cgacaccgaa aaagacatcc tcgatttaaa
     3361 tataggcatc aactccgtcg gcgcggaaga cgtactgcaa accgcgttca aaggcaggtt
     3421 ggacggcagc atcggcatcg gtggcacgac cgcctcgccc aaaatctctt ggcaactcgg
     3481 catcggcacg gcgcgcacgg acggcagcct cgccattgca agcgacccag caaacggaca
     3541 gcggaaactg gtgctcgaca ccgtcaacat cgccgccggg caaggcagcc tgaccgcgca
     3601 aggctatctc gagctgttta aagaccgcct gctcaagctg gacatccgtt cccgcgcatt
     3661 cgacccttcg cgcatcgatc cgcaacttcc ggcaggcaat atcaacggct caataaacct
     3721 tgccggcgaa ctggcaaaag agaaattcac aggcaaaatg cggtttttac ccggcacgtt
     3781 caacggcgta ccgattgccg gcagtgccga cattgtttac gagtcccgcc accttccgcg
     3841 tgccgccgtc gatttgcggc tggggcggaa cattattaaa acagacggcg gcttcggcaa
     3901 aaaaggcgac cggcttaacc tcaatatcac cgcacccgat ttatcccgtt tcggtttcgg
     3961 actcgcgggg tctttaaatg tacgcggaca cctttccggt gatttggacg gcggcatccg
     4021 aacctttgaa accgaccttt ccggcgcggc gcgcaacctg cacatcggca aggcggcaga
     4081 catccgttcg ctcgatttca cgctcaaagg ttcgcccgac acaagccgcc cgatacgcgc
     4141 cgacatcaaa ggcagccgcc tttcgctgtc gggcggagcg gcggttgtcg ataccgccga
     4201 cctgatgctg gacggcacgg gcgtgcagca ccgcatccgc acacacgccg ccatgacgct
     4261 ggatggcaaa ccgttcaaat tcgatttgga cgcttcaggc ggcatcaaca gggaacttac
     4321 ccgatggaaa ggcagcatcg gcatcctcga catcggcggc gcattcaacc tcaagctgca
     4381 aaaccgtatg acgctcgaag ccggtgcgga acgcgtggcg gcaagtgcgg caaattggca
     4441 ggcaatgggc ggcagcctca acctgcaaca cttttcttgg gataaaaaaa ccggcatatc
     4501 ggcaaaaggc ggcgcacacg gtctgcatat cgccgagttg cacaatttct tcaaaccgcc
     4561 cttcgaacac aatctggttt taaacggcga ctgggatgtc gcctacgggc gcaacgcgcg
     4621 cggctacctc aatatcagcc ggcaaagcgg cgatgccgta ttgcccggcg ggcaggcttt
     4681 gggtttgaac gcattttccc tgaaaacgcg ctttcaaaac gaccgcatcg gaatcctgct
     4741 tgacggcggc gcgcgtttcg ggcggattaa cgccgatttg ggcatcgcca acgccttcgg
     4801 cggcaatatg gcaaatgcac cgctcggcgg caggattacc gcctcccttc ccgacttggg
     4861 cgcattgaag ccctttctgc ccgccgccgc gcaaaacatt accggcagcc tgaatgccgc
     4921 cgcgcaaatc ggcggacggg taggctctcc gtccgtcaat gccgccgtca acggcagcag
     4981 caactacggg aaaatcaacg gcaacatcac cgtcgggcaa agccgctctt tcgataccgc
     5041 gcctttgggc ggcaggctca acctgaccgt tgccgatgcc gaagtattcc gcaacttcct
     5101 accggtcgga caaaccgtca aaggcagcct gaatgccgcc gtaaccctcg gcggcagcat
     5161 cgccgatccg cacttgggcg gcagcatcaa cggcgacaaa ctctattacc gcaaccaaac
     5221 ccaaggcatc atcttggaca acggctcgct gcgttcgcat atcgcgggca ggaaatgggt
     5281 aatcgacagc ctgaaattcc ggcacgaagg gacggcggaa ctctccggta cggtcggtat
     5341 ggaaaacagc ggacccgatg tcgatatcgg cgcggtgttc gacaaatacc gcatcctgtc
     5401 ccgccccaac cgccgcctga cggtttccgg caacacccgc ctgcgctatt cgccgcaaaa
     5461 aggcatatcc gttaccggga tgattaaaac ggatcagggg ctgttcggtt cgcaaaaatc
     5521 ctcgatgccg tccgtcggcg acgatgtcgt cgtattaggc gaagtcaaaa aagaggcggc
     5581 ggcaccgctc cccgtcaata tgaacctgac tttagacctc aatgacggca tccgcttcgc
     5641 cggctacggc gcggacgtta ccataggcgg caaactgacc ctgaccgccc aatcgggcgg
     5701 aagcgtacgg ggcgtgggca cggtccgcgt catcaaaggg cgttataagg catacgggca
     5761 ggatttggac attaccaaag gcacggtctc ctttgtcggc ccgctcaacg atcccaacct
     5821 caacatccgc gccgaacgcc gcctttcccc cgtcggtgcg ggcgtggaaa tattgggcag
     5881 cctcaacagc ccgcgcatta cgctgacggc aaacgaaccg atgagtgaaa aagacaagct
     5941 ctcttggctc atcctcaacc gcgccggcag cggcagcagc ggcgacaatg ccgccctgtc
     6001 tgcagccgca ggtgcgctgc ttgccgggca aatcaacgac cgcatcgggc tggtggatga
     6061 tttgggcttt accagcaagc gcagccgcaa cgcgcaaacc ggcgaactca accccgccga
     6121 acaggtgctg accgtcggca aacaactgac cggcaaactc tacatcggct acgaatacag
     6181 catctccagc gcggaacagt ccgtcaaact gatttaccgg ctgacccgcg ccatacaggc
     6241 ggttgcccgt atcggcagcc gttcgtcggg cggcgagctg acatacacca tacgtttcga
     6301 ccgcttctcc ggttcggaca aaaaagactc cgccggaaac ggcaaaggaa aataagcggt
     6361 tttcagacgg cgcgccgcca aaccggacat ttgaaaacct gcttttccac cgtccgccgc
     6421 cgccgtccgc ctgcaaggga acagaatcga tatagtg
//
LOCUS       DAWGRZ010000120         6440 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000120 DAWGRZ010000000
VERSION     DAWGRZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.120"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            143..1684
                     /locus_tag="WHT44_001551"
     CDS             143..1684
                     /locus_tag="WHT44_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ2533717.1"
                     /translation="MDGRRWVVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVYAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLAAFALGIFLLLDGGEGGK
                     QTETL"
     gene            1713..2312
                     /locus_tag="WHT44_001552"
     CDS             1713..2312
                     /locus_tag="WHT44_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ2533718.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            2309..2914
                     /gene="nadD"
                     /locus_tag="WHT44_001553"
     CDS             2309..2914
                     /gene="nadD"
                     /locus_tag="WHT44_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ2533719.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            2973..3359
                     /gene="rsfS"
                     /locus_tag="WHT44_001554"
     CDS             2973..3359
                     /gene="rsfS"
                     /locus_tag="WHT44_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ2533720.1"
                     /translation="MNEQELQDLQKMVGVAVNALGDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     CDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            3413..3883
                     /gene="rlmH"
                     /locus_tag="WHT44_001555"
     CDS             3413..3883
                     /gene="rlmH"
                     /locus_tag="WHT44_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ2533721.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVFLTEQLYRAVSILHNHPYHRE"
     gene            3956..4222
                     /locus_tag="WHT44_001556"
     CDS             3956..4222
                     /locus_tag="WHT44_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ2533722.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            4379..5062
                     /locus_tag="WHT44_001557"
     CDS             4379..5062
                     /locus_tag="WHT44_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ2533723.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPASAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            5186..5698
                     /gene="coaD"
                     /locus_tag="WHT44_001558"
     CDS             5186..5698
                     /gene="coaD"
                     /locus_tag="WHT44_001558"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ2533724.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            5730..6386
                     /locus_tag="WHT44_001559"
     CDS             5730..6386
                     /locus_tag="WHT44_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ2533725.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNKESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRCGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFDYQNRQIEIVAPLKNEWQTENIHRALEEFCMENKID
                     "
BASE COUNT         1398 a         1696 c         1862 g         1484 t
ORIGIN      
        1 cgccttgtcc tgatttttgt taatccacta tatttcagac ggcgggagga aggcggatac
       61 ggtatgtagt cggattgaaa tatacggtat gtgggggctt ttgggtacaa tcgcaacatc
      121 agtgtttcaa gtggggaagg cgatggatgg acggcgttgg gtggtatggg gtgcttttgc
      181 cctgctgcct tcggcttttt tggcggtaat ggtcgttgcg cctttgtggg cggtggcggc
      241 gtatgacggt ttggcgtggc gcgcggtgct gtcggatgcc tatatgctca aacgtttggc
      301 gtggacggta tttcaggcag cggcaacctg tgtgctggtg ctgcctttgg gcgtgcctgt
      361 cgcgtgggtg ctggcgcggc tggcgtttcc ggggcgggct ttggtgctgc gcctgctgat
      421 gctgcctttt gtgatgccca cgttggtggc gggcgtgggc gtgctggccc tgttcggggc
      481 ggacgggctg ttgtggcgcg gcaggcagga tacgccgtat ctgttgttgt acggcaatgt
      541 gtttttcaac cttcctgtgt tggtcagggc ggcgtatcag gggtttgtgc aagtgcctgc
      601 ggcacggctt cagacggcac ggacgttggg cgcgggggcg tggcggcggt tttgggacat
      661 tgaaatgccc gttttgcgcc cgtggcttgc cggcggcgtg tgccttgtct ttctgtattg
      721 tttttccggg ttcgggctgg cgctgctgct gggcggcagc cgttatgcca cggtcgaagt
      781 ggaaatttac cagttggtca tgttcgaact cgatatggcg gttgcttcgg tgctggtgtg
      841 gctggtgttg ggggtaacgg cggcggcagg gttgctgtat gcgtggttcg gcaggcgcgc
      901 ggtttcggat aaggcggttt cccctgtgat gccgtcgccg ccgcagtcgg tcggggaata
      961 tgtgctgctg gcgtttgcgg cggcggtgtt gtctgtgtgc tgcctgtttc ctttgttggc
     1021 aattgttgtg aaagcgtggt cggccggcga atcgtggcgt gtgttaatgg aaagtgaaac
     1081 gtggcaggcg gtgtggaata ctttgcgctt ctcggcggcg gcggtgtatg cggcggcggt
     1141 tttgggtgtg gtgtatgcgg cggcggcgcg gcggtcggcg tggatgcgcg ggctgatgtt
     1201 tttgccgttt atggtgtcgc cggtttgtgt ttcggcgggc gtgctgctgc tttatccgca
     1261 gtggacggct tcgttgccgt tgctgctggc gatgtatgcg ctgctggcgt atccgtttgt
     1321 ggcaaaagat gttttatcag cctgggatgc actgccgccg gattacggca gggcggcggc
     1381 gggtttgggt gcaaacggct ttcagacggc atgccgcatc acgttccccc tcttgaaacc
     1441 ggcgttgcgg cgcggtctga ctttggcggc ggcaacctgc gtgggcgaat ttgcggcgac
     1501 attgtttctg tcgcgtccgg aatggcagac gctgacgact ttgatttatg cctatttggg
     1561 acgcgcgggt gaggataatt acgcgcgggc gatggtgctg acattgctgt tggcggcgtt
     1621 cgcgctgggt attttcctgc tgttggacgg cggcgaaggc ggaaaacaga cggaaacgtt
     1681 ataatgtgaa ccctttttca gaggacggca aaatgagcga gttggataac atccttgccc
     1741 ataaccggca gtttgtcgag tcgggcgaat atgaaaaata ctttaccgac aaataccccg
     1801 aacgcgggct ggcagttttg tcctgtatgg atgcgcggat tatcgggctg ctgcccgacg
     1861 cgttgggttt gaaaaacggc gatgccaagc tgattaaaaa tgccggcgcg ctggttacgc
     1921 acccgtgggg ttcggtgatg cggagccttt tggttgccgt gtttgaactg aaggtcagag
     1981 agattatggt catcgcccat cacgattgcg gtatgcaggg gctgaatgcc gaagaattcc
     2041 tcgggcgcgt ccgggaaagc cggattcccg aagaccgtat cgaaaccctg cgttatgccg
     2101 gtatcgacct cgacggctgg ctgaccggtt tcgacaacgt cgaagacagc gtgcgccaca
     2161 cggtggacct tatccgtaac catccgctga tgccgcgcca tatcgccgtt cacggactgg
     2221 tcatccatcc cgttaccggc aaactgacgc tggttgtgga cggcagtgtt tcagacggca
     2281 tggacttatc ggaaggaatg gaaacatcat gaagaaaatc ggattgttcg gcggtacttt
     2341 cgacccgata cacaacggac atcttcatat cgcccgtgcc tttgccgacg aaatcggctt
     2401 ggacgcggtt gttttcctgc cgacaggcgg cccgtatcac aaagacgcag cctccgcttc
     2461 cgccgccgac cgccttgcca tggtcgaatt ggcgacggca gaagacgcgc gttttgccgt
     2521 cagcgattgc gacattgtcc gagaaggtgc aacctatact tttgataccg tccaaatctt
     2581 ccgccagcag ttcccatccg cgcaactctg gtggctgatg ggcagcgaca gcctgatgaa
     2641 gctgcacaca tggaaaaaat ggcagatgct cgtgcgcgaa accaatatcg ccgtcgccat
     2701 gaggcagggc gacagcctgc accaaacccc gcgcgaactg cacgcgtggc tgggcaagtc
     2761 ccttcaggac ggcagcgtcc gcatcttgtc cgccccgatg cataatgtgt cgtcaacgga
     2821 aatccgccgc aaccttgccg gccaaggcgt ttcagacggc atcccgcctg ccgccgcacg
     2881 ctacatccgc gaacacggtt tgtatgaaaa ataaagtcaa atcagtaaga aacggctata
     2941 atgccgtctg aaaacatccc gttaggaaaa taatgaacga acaagaactg caagacctgc
     3001 aaaaaatggt cggggtcgcc gtcaacgccc tcggagacat caaagccaaa gacatttccg
     3061 ttctcgaaac ccaagacaaa acttcgctgt ttgccagaat gattatcgcc agcggcgaca
     3121 gtacgcgcca agtcaaagca ctggccaaca acgttgccgt cgatttgaaa gaagccggtt
     3181 ttgaaatcct cagtaccgaa ggcgacagcg gcgaatggac gctggttgat gcaggagacc
     3241 tcgtcgtcca cgtcatgctc cctgccgtgc gcgacttctg cgacatcgac accctctggg
     3301 gcggcgagaa accgagtttc cacgccggaa tgcagaagcc gtggcacgcg gcagactgat
     3361 tcccgatgcc gtctgaacct tcagacggca ttttcctgta agggagaaag cattgaacat
     3421 caccgttttg gcagtcggca ccaaaatgcc gcgctgggtt gatgaggccg tcgccgaata
     3481 cgccaaacgc ttcggacgcg acgtcgccta cgcactcaaa gaaatcaaac ccgaaaaacg
     3541 cggcgcgggc gtgaatgccg cccaaggtat ggcggcggaa gaaaaacgca tccttgaagc
     3601 cattccgcaa ggcgcgttcc tcgtcgttct tgacgaacgc ggcaaagcac cgacctccgt
     3661 cgagctggcg gaacacctca aaagctggcg gcaaaacggc gaacacgtct gcttcgtcat
     3721 cggcggcgcg gacggcatga ccgaccgcct caaacagcag gcacgcatga tgatgcgcct
     3781 gtccagcctg accctgccgc acggcatggt gcgcgtcttt ctgaccgagc agctctaccg
     3841 cgccgtttcc atcctgcaca accatcccta tcatcgggaa taagaaggct tttgtccgta
     3901 tccctaatcg gttaaaatca ccccgttatt tctgaacaca gcaaaggaat ccgctatggc
     3961 acgcatggta ttctgcgtca agctcaacaa agaagccgaa ggcatgaaat ttccgccgct
     4021 tcccaacgaa ttgggcaagc gcatttttga aaacgtatcg caagaagcgt gggcggcgtg
     4081 gacgcgccac caaacgatgc tgattaacga aaaccgttta agcctcgccg atccgcgcgc
     4141 gcgcgaatac ctggctcagc agatggagca gtatttcttc ggcgacggcg cggatgccgt
     4201 tcagggatac gttccgcaat aacggttttc cgttttgaac acaggctgtc cgaaactgct
     4261 tcagacggcc tttaaaatac gccggacaac tttatcttca cgcaaccgga caaattttga
     4321 cattgggcaa tgttaaaatc ccccattatt cccaaccgcg ctttcaggag cagatgatat
     4381 gcaacacgac gtttacgact acaccgcgca tacggtttct aaaaacaccg tcctgcagaa
     4441 aacctaccgc ctgctcggat tttcatttat tccggcttcc gcaggcgcgg cacttgccgc
     4501 caatgccggt ttcaattttt acgccgcctt cggttcgcgc tggatagggt ttgccgtcgt
     4561 gttggcgttt ttctacggta tgatccactt catcgagaaa aaccgttaca gcaataccgg
     4621 cgttaccctg ctgatggtat tcacattcgg tatgggcgta ttgatcggcc ccgtgctgca
     4681 atacgcgctc catattgccg acggtgcgaa aatcgtcggc attgccgccg cgatgaccgc
     4741 cgccgtcttt ttaacgatgt ccgccttggc gcgccgaacc cggctcgata tgaacgcgct
     4801 cggacgcttc ctgaccgtag gtgcggtcat tctgatggtc gccgtggttg ccaatctgtt
     4861 tttgggtatt cccgcactcg ccctgaccat ttccgccggt tttgtcttgt tcagttcctt
     4921 aatgattatg tggcaggtac gcaccgtcat cgacggcggc gaagacagcc acatcagcgc
     4981 ggcactgaca ctgtttatct cgctttacaa catcttcagc agcctgctga acatcctgtt
     5041 gtctttaaac ggcgaggatt gatgccggta aaacgccgtc cgactatgcc gtctgaaaat
     5101 gcttcagacg gcattttact ttgggcatac aatacggatg cacacgcacc aacacacagc
     5161 cgactatgaa gccgggagga atcaaatgtt accgaataca ccgcgccgcg ccgtttatgc
     5221 cggcagtttc gatccgccca cattggggca tctgtggatg atacggcagg cgcaatctat
     5281 gtttgacgaa ctcatcgtcg ccatcggcat taaccccgac aaacgcagca cctataccgt
     5341 cgctgaaagg caggatatgt tgtgcgatat tactaaaatg tttcccaacg tcagaaccga
     5401 tgtatttgaa aaccgatttc tggtgcatta cgcccgtgag gtagatgcag gattcatcgt
     5461 gcgcggcatc cgttctgctt cggattacga atacgaacgt tccatgcgcc atatcaacag
     5521 cgacctcgcc cccgaaatat ccaccgtatt cctcatgccg ccgcgcgaaa tcgccgaagt
     5581 gtcgtccact atggtcaaag gactggtcgg gccggaaggc tggacggaaa cgatccaccg
     5641 ctacgtgccg caagctgtgt acgaaaaaat ccttgccgaa catcaacacg aaaattgaac
     5701 ccttctattt ttgcaaggca tatatggata tgttggaaat tttgttccgc catcaagact
     5761 tcgtcgccat caacaaaccg ggcggcatat ccgtccacca agacagcggc gagaccggac
     5821 tggcccgaac actcgccata cagttgggcg tagagcgtgt gtggctgctg caccgattgg
     5881 acaaacagac cagcggcatt ttactgtttg ccctcaacaa agaaagcgcg tccgccctta
     5941 gcggacagtt tgccggcaaa agcatcaaaa aaacctattt ggcactgtcc gaccgcaagc
     6001 cgtccaaaaa gcagggttgg attaaaggcg gaatggaaaa atccagatgt ggaatgtgga
     6061 agttgacgcg caatacagaa aatatcgccg ttacccgatt ccacagcatc agtatcgccg
     6121 aaaaactgcg gctgttcatc ctagaaccgc atacgggcaa aacgcaccaa ttgagagtgg
     6181 cgatgaaaag tttgggcagt ccgattctgg gcgacagctt atacggcgga acagaatccg
     6241 aaaccatgtt tctgtatgca tggaaaatac aatttgacta ccaaaaccga caaatcgaaa
     6301 ttgttgcacc cttaaaaaac gaatggcaga ctgaaaatat ccatcgggca ctggaagaat
     6361 tctgtatgga aaataaaatt gactgatata aattaaattt ataaacaaat agataaatat
     6421 ttttaaaaaa atttcttcag
//
LOCUS       DAWGRZ010000121         6275 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000121 DAWGRZ010000000
VERSION     DAWGRZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.121"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            80..1915
                     /gene="edd"
                     /locus_tag="WHT44_001560"
     CDS             80..1915
                     /gene="edd"
                     /locus_tag="WHT44_001560"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ2533726.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYASMPKSIKIEMLQDTVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVSGELNVLINETE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
     gene            2096..2734
                     /locus_tag="WHT44_001561"
     CDS             2096..2734
                     /locus_tag="WHT44_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ2533727.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAIDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPEYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            2749..3045
                     /locus_tag="WHT44_001562"
     CDS             2749..3045
                     /locus_tag="WHT44_001562"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533728.1"
                     /translation="MPSEKPFPFQTAFCRLRAQSAYTAALIRHTAPKMPIRLPHNKNN
                     VQFIRQQPDSPYYAAEKMPSETHSDGIHLKKTTDSTPINPLPKPLSSLGFRRRV"
     gene            complement(2993..4033)
                     /locus_tag="WHT44_001563"
     CDS             complement(2993..4033)
                     /locus_tag="WHT44_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEZ2533729.1"
                     /translation="MKAMVYHGANDIRFEEKPRPQIIDPTDAVVKIVKTTICGTDLGI
                     WKGKNPEVADGRILGHEGIGIVEEVGEAVKNIKVGDKVIISCVSKCCTCDNCKTQLYS
                     HCRNGGWILGYMIDGTQAEYVRTPYADNSLVPLPDNVNEEIALLLSDALPTAHEIGVQ
                     YGDVKPGDTVFIAGAGPVGMSALLTAQLYSPAAIIVCDMDENRLKLAKELGATHTINP
                     ASGEVSKQVFAIVGEDGVDCAIEAVGIPATWNMCQDIVKPGGHIAVVGVHGQSVDFKL
                     EKLWIKKLAITTGLVNANTTEMLMKAISSSSVDYTKMLTHHFKFSELEKAYDVFKHAA
                     ENQVMKVVLEAD"
     gene            4196..5236
                     /gene="mutY"
                     /locus_tag="WHT44_001564"
     CDS             4196..5236
                     /gene="mutY"
                     /locus_tag="WHT44_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ2533730.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIIRNRNGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            5653..6213
                     /locus_tag="WHT44_001565"
     CDS             5653..6213
                     /locus_tag="WHT44_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ2533731.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
BASE COUNT         1622 a         1747 c         1505 g         1401 t
ORIGIN      
        1 accgtctgaa tacccgatac aaaaatcaga aacgcacaaa caaatcccca ataccccccg
       61 ttccgacagg agaccgaccg tgaacactac tcctatccac tccaaactcg ccgaaatcac
      121 cgggcgcatt attgaacgca gccgtccgac gcgtgaaaaa tatctggcga aaatccgtag
      181 tgccaaacag atgggacgct tagagcgcaa ccaactcggc tgcagcaatt tggcgcacgg
      241 ctacgcctcc atgcccaaaa gcatcaaaat cgaaatgctt caggacaccg ttcccaactt
      301 aggcatcatc accgcctaca acgacatggt ttccgcacac cagccgttta aagacttccc
      361 tgaccaaatc aaagacgaag cgcagaaaaa cggcgcaacc gcgcaagttg ccggcggtac
      421 gcccgccatg tgtgacggta ttacccaagg ctatgccggc atggaattgt cgctgttctc
      481 ccgcgacgta attgccatga gtactgctgt cggtctgtct catcaaatgt ttgacggcag
      541 cctgtttatg ggcgtgtgcg acaaaatcgt tcccggtttg atgatcggtg cgttgtcttt
      601 cggccatatt ccgggtatct tcgtccccgc aggcccgatg tccagcggta tcggcaacaa
      661 agaaaaagcc cgcacccgcc agcttttcgc cgaaggcaag gtcggacgca acgaactttt
      721 gaaaagcgaa atgggttctt accacagccc gggcacctgc actttctacg gcacggcaaa
      781 ctccaaccaa atgatgatgg aaatgatggg cgtgcacctg cctgccgccg ccttcgtcca
      841 cccttacacc gacctgcgtg aagccctgac ccgctatgcc gccggacacc tcgcgcgcgg
      901 catcaaaaac ggcacgatta aacctttggg cgaaatgttg accgaaaaat cctttatcaa
      961 cgccttgatt ggcctgatgg caaccggcgg ttcgaccaac cacaccatgc acctcgtcgc
     1021 tatggcgcgt gcggccggcg tgattttgaa ctgggacgac ttcgacgaaa tttcctccat
     1081 catcccgctg ctcatccgcg tttatccgaa cggcaaggcc gacgtgaatc actttaccgc
     1141 agcgggcgga ctgcctttcg ttatccgcga attgctgaat gcaggcctgt tgcacgacga
     1201 tgtcgatacc gtcgtcggac acggtatgcg ccactacacc aaagagcctt tccttatcga
     1261 cggcaaactc gaatggcgcg aagcccccga aaccagcggc aacgacgaca tcctgcgcaa
     1321 agctgacaac ccgttctccc ccgacggcgg tctgcgcctg atgaaaggca acatcggacg
     1381 cggcgtgatt aaagtgtccg ccgtgcgcga aggctgccgc attattgaag cgcctgccat
     1441 cgtgttcaac gaccaacgcg aagtgttggc tgcgtttgaa cgcggcgagt tggaacgcga
     1501 ttttgtgtgc gtcgtccgct accaaggccc gcgtgccaac ggtatgcccg aattgcacaa
     1561 actgaccccg cctttgggca tcctgcaaga ccgcggcttc aaagtggcgc tgctgaccga
     1621 cggccgtatg tccggcgcgt ccggcaaagt tccagcctcc atccacatga cacccgaagc
     1681 cctgatgggc ggcaacatcg ccaaaatccg taccggcgac ctgatccgct tcgactccgt
     1741 tagcggcgaa ctcaacgtcc tgattaacga aaccgaatgg aatgcccgcg aagtcgaaag
     1801 catcgacttg ggcgcgaacc aacaaggctg cggccgcgaa ctcttcgcca acttccgcag
     1861 catgaccagc agcgcggaaa ccggtgccat gagtttcggc ggcgaatttg cctgatgcgc
     1921 gtttcagacg gccttttcag accgaaggcc gtctgaaaaa ttattcaagc gttttaagat
     1981 agacgtaggt tggattctcg aatccgacac agccgtccaa gatgtcggtt tcttgaatcc
     2041 gacctacaac ctgtcccatc ttaataaaat accccattcc acccggagaa ccgaaatgtc
     2101 caaactgacc ccccgcgaaa ttttgaccgc cggcgcagtt gtgccggtaa tggcgattga
     2161 cgacttaagc accgccatcg atttgtccca cgcccttgtc gaaggcggca tccctaccct
     2221 cgaaatcacc ctgcgcaccc ctgtcggcct cgatgccatc cgcctgattg ccaaagaagt
     2281 gcccaacgcc atcgtcggcg caggtacggt aaccaatccc gaacagctca aagccgtcga
     2341 agacgcaggc gcggttttcg ccatcagccc ggggctgcat gaatccctcg ccaaagccgg
     2401 ccacaacagc ggcatccccc tgattcccgg tgttgccacc ccgggcgaaa tccaactggc
     2461 tttggaacac ggcatcgaca ccctcaaact cttccccgcc gaagtcgtcg gcggcaaagc
     2521 catgctcaaa gccctgtacg gcccttacgc cgatgttcgc ttctgcccga caggcggcat
     2581 cagcctcgcc accgcgcccg agtacttggc actgcccaac gtcctgtgcg tcggcggctc
     2641 ttggctgaca ccgaaagaag ccgtgaaaaa caaagactgg gacaccatca cccgcctcgc
     2701 caaagaagcg gcggcgttga aacccaaagc ctgattcgca tcgtaaaaat gccgtctgaa
     2761 aaacctttcc cgtttcagac ggcattttgc cgattgaggg cacagtcggc atacacggca
     2821 gcactgatca gacataccgc ccctaaaatg cccatccgcc ttccgcataa taaaaataac
     2881 gttcagttca ttcgacagca gccggacagc ccatactacg cggctgaaaa aatgccgtct
     2941 gaaacgcatt cagacggcat ccacttaaaa aaaacaactg attcaacgcc gattaatccg
     3001 cttccaaaac cactttcatc acttggtttt cggcggcgtg tttgaacacg tcgtaggctt
     3061 tttccaattc actgaatttg aaatgatggg tcagcatttt ggtgtaatcg acggagctgc
     3121 tggaaatcgc cttcatcagc atttcggtgg tattggcgtt taccagaccg gtagtgatgg
     3181 caagcttttt aatccagagt ttttccagtt tgaaatcaac ggattgacca tgtacaccaa
     3241 ccacagcgat atggccgccg ggtttcacaa tgtcttggca catattccat gtagcaggga
     3301 taccgacggc ttcgatggcg caatccacgc cgtcttcgcc gacgatggca aagacttgtt
     3361 tggatacttc gccggaagca gggttaatgg tatgggtcgc acccaattct ttcgccagtt
     3421 tcaaacggtt ttcgtccata tcgcaaacga tgatggcggc gggactgtac agttgggcgg
     3481 tcaacagggc ggacataccg acagggcctg ccccagcgat gaatacggtg tcgccgggtt
     3541 tgacatcgcc gtattgcacg ccgatttcgt gggcggtcgg caaagcgtcg ctcaacaaca
     3601 gggcgatttc ttcgttgaca ttatcgggca gcggaacgag gctgttgtcg gcataaggcg
     3661 tacggacgta ttcggcctga gtaccgtcaa tcatgtaacc caaaatccaa ccgccgttac
     3721 ggcagtgtga atagagttgg gttttgcagt tgtcgcaagt gcaacatttg ctgacgcatg
     3781 aaataatgac tttatcgccg actttgatgt tttttacagc ctcgccgact tcttctacaa
     3841 taccgatgcc ctcatgaccg agaatacggc cgtcggcaac ttcggggttt ttgcctttcc
     3901 aaatacccaa atcggtaccg caaatcgtgg ttttgacgat tttcaccacc gcatcggtcg
     3961 gatcgataat ctgcggacgg ggtttttctt caaaacggat gtcgtttgcg ccgtgataaa
     4021 ccattgcttt catgctgata ctccttgctt gttgataaat aatttcaata ccgcaataaa
     4081 gtttctttat atgagttata tgcccctaca aaaaataagt caataagaat tattttcaca
     4141 atgttataca ataacatacc gttttaaata taaataaaac caccgattga tattaatgaa
     4201 cacacccatc cccttctccg aacggctcat ccgctggcaa aaacaacacg gtcgccacca
     4261 cctcccttgg caggtcaaaa acccttattg cgtctggctt tccgaaatca tgctccagca
     4321 aacgcaagtc gccaccgtgt tggactacta tccgcgcttc ttagaaaaat tcccgaccgt
     4381 tcagacgctt gccgccgcgc cgcaagacga agtgttgtcg ttgtgggcgg gcttgggcta
     4441 ttacagccgc gcgcgcaacc tgcacaaagc cgcgcaacaa gtcgtcaggc aattcggcgg
     4501 cacgtttccg tcggagcgca aagacttgga aaccctctgc ggcgtaggca gaagcaccgc
     4561 cgccgccatt tgcgccttct ccttcaaccg ccgcgaaacc attttggacg gcaacgtcaa
     4621 acgcgtactc tgccgcgtgt tcgcccgcga cggcaatccg caggacaaaa aatttgaaaa
     4681 ctcgctctgg acacttgccg aaagcctgct gccgtctgaa aacgccgata tgcctgccta
     4741 tacacaaggt ttgatggatt tgggcgcgac cgtgtgcaaa cggacgaaac ccttgtgcca
     4801 ccaatgcccg atggcggaca tctgcgaagc gaaaaagcaa aaccgcaccg ccgagctgcc
     4861 gcgcaaaaaa accgccgccg aagtaccgac cctgccgctt tactggctga ttatccgcaa
     4921 ccggaacggc gcgattttgc tggaaaaacg ccccgccaaa ggcatttggg gcgggctgta
     4981 ttgcgtgccg tgttttgaaa gtttgaacgg gctttccgac tttgccgcca aattctccct
     5041 gaccatggca gatatggacg aacaaaccgc cctgacccac cggctgacgc accggttgct
     5101 gatgattacg ccgtttgaag gacaaatgcc gtctgaaagc ccttcagacg gcatttggat
     5161 aaagccggcg catttgaaag attacggttt gcccaagcct ttggaaattt atttaaacgg
     5221 taataggtta gaataaacaa aataaaccca ttgaactgtt gtttgcaggt atcgcagcaa
     5281 gaacaaccga tgaatttggg tcgtatttta ggcggcggga taatgttcaa atgggacatt
     5341 tggaacggta gaagtcggca atttaaaaag gatttaaaaa gcaaagaagg tcaaaaacat
     5401 gaacacaaac ttaaatgaca aagacaaagc catggatacc gcaatcaggt ttcagaaaag
     5461 gatgaggatt ccgaaatttt tctttttaat tctctgaatc acaatggttt tggcatttat
     5521 ccaagacgtg ataacgggtt ctaattttct gcaaataaca attaatgtaa aattttcgta
     5581 aaaatttatc ggcttttaaa acaaaattga ctaaaatagt cgcgagtttt tactgcaata
     5641 aaggagattg caatgaatat gaaaacctta ttagcactag cggttagtgc agtatgttca
     5701 gttggtgttg cgcaagcaca cgagcataat acgataccta aaggtgcttc tattgaagtg
     5761 aaagtgcaac aacttgatcc agtaaacggt aacaaagatg tgggtacagt gactattact
     5821 gaatctaact atggtcttgt gtttacccct gatttacaag gattaagcga aggcttacat
     5881 ggtttccaca tccatgaaaa cccaagctgt gagccaaaag aaaaagaagg taaattgaca
     5941 gctggtttag gcgcaggcgg tcactgggat cctaaaggtg caaaacaaca tggttaccca
     6001 tggcaagatg atgcacactt aggtgattta cctgcattaa ctgtattgca tgatggcaca
     6061 gcaacaaatc ctgttttagc accacgtctt aaacatttag atgatgttcg cggtcactct
     6121 attatgatcc acacgggtgg tgataatcac tccgatcatc cagctccact tggcggtggc
     6181 ggcccacgta tggcatgtgg cgtgattaaa taattcgatt gttcgaaacg aaaagtgcgg
     6241 taaattttga ccgcactttt ttgctagata tttag
//
LOCUS       DAWGRZ010000122         6262 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000122 DAWGRZ010000000
VERSION     DAWGRZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.122"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(142..2022)
                     /locus_tag="WHT44_001566"
     CDS             complement(142..2022)
                     /locus_tag="WHT44_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEZ2533732.1"
                     /translation="MNIFYEESGQFKVAAIVQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFSAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGMKRVGIHIAAPS
                     LAIKQGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGTYRPSISIYFDVDNE
                     FNVGAPTCKIEAVNIATNLRIQAIEPHFNAETGLDEAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDEEGKVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
     gene            2178..2810
                     /locus_tag="WHT44_001567"
     CDS             2178..2810
                     /locus_tag="WHT44_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ2533733.1"
                     /translation="MGNTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFAFRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAASLFAISDDEDEIVAYLSEHGLQTA"
     gene            2882..4252
                     /gene="purB"
                     /locus_tag="WHT44_001568"
     CDS             2882..4252
                     /gene="purB"
                     /locus_tag="WHT44_001568"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ2533734.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIGKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMSNAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIESLEIPVEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            4332..6200
                     /locus_tag="WHT44_001569"
     CDS             4332..6200
                     /locus_tag="WHT44_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ2533735.1"
                     /translation="MQTVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLCHISIILFLILTASSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFPARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLIKDIPNV
                     HWVDAQKYLPKNTVEIYGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSHGGALQ"
BASE COUNT         1350 a         1634 c         1702 g         1576 t
ORIGIN      
        1 gattgcgggt atttatcggg aacggcggct tggaagttca ttgaaacgga aaaacaacgg
       61 aaacccaaaa aaccggattc ccgactgtgg gaatgatgag attcaggttt ctgtttttgc
      121 cggagtttgc cgtatcgggc ttcagacggc attgcctgcc gttgtacccg cgggtgcgac
      181 tgccttgatg tagttgagcg agacaaactg cttctcggca tccaattcgg tgattttgaa
      241 caatgcctgt gatttgggca gtgcgtcaaa cggaataccg gtcgcgcgcg tgaccagcgg
      301 caggccttcg atgcggacga ggtcttcttt gaggatggtc gcggtcagct cgcttgtacc
      361 ttgctgttgc aggtacacaa ggctccagta ggcttccatc tgccgttgga aatcggcgta
      421 ggcggtatag gcggcatcaa agtcgcgcag tgcggcgaaa agctcggcat cgctgttttg
      481 atacagcggc tcggcagtgt cgtctatcag gctgatcagc tgcttttggt tgatgtagtc
      541 ggcggcgcgg cgcagcggcg aggtaaacca gccgtaatgc tgcacgccca tgccgatatg
      601 cggctcggat ttggtgctca tgcgtacttt tccggtgggt tggacgcgga agaggccggg
      661 caggtcgttg tcatggagca tttgtgccca agtgctgttg gcaagaatca tcatctcgct
      721 gaccagcgta tcgatgggtg agccgcgttc gcggcggacg acggatacct tgccttcctc
      781 atccaattcg atgctgtaat cgtattgcgg cgcgcggtcg ggttcgtatt tgccgcgcgc
      841 tttttgcagg gcggtggcga attgatagaa ccaaatcagg tcttgatggt gggcgaacat
      901 catttcgccg gcttcgtcca agccggtttc ggcgttgaaa tgcggctcga tggcttggat
      961 acgcaggttt gtggcgatgt tgaccgcttc gattttgcag gtcggcgcgc cgacgttgaa
     1021 ctcgttgtcc acatcgaaat aaatgctgat ggaagggcgg tatgtacctg catcaaggct
     1081 gaacgcggca atccagtttt cgggcaacat cgtgattttg ccgccgggaa aataaaccgt
     1141 gcttaagcgt tccatgatgt ttttttccat tttgtcgcct tgcttaatgg caagcgacgg
     1201 cgcggcgatg tggataccga cgcgtttcat gccattgccc aagtcggtca ggctcaaggc
     1261 atcatcgact tcggtggtcg actcgtcgtc aatggaaaag gcggtaacgt cggccttggg
     1321 caggtcgggc atttcaggaa gcggaaggtc ggggaagcct gttcctttag ggaaatattt
     1381 gatttcaaac ccgtcttgca ggtattgggg aatggacgta atgccgcccg tttttttcgc
     1441 caattcgtag gcagaggttt tcagcgcgtc ggcggctttg gtaaaggctt tgtaggtcag
     1501 cgactgcttg tcgggcgcgt gcaggatggt tttcaaatcc gccgcgattt cagacggcat
     1561 ctcgccgcgt ttcaaggctt ccgcccaagc gtcgatttgc gcgtcttgct gttttttgcg
     1621 ttcgatggcg gcaagtgctt gttttaaagt ttcttcgggc gcggctttga acacgccttt
     1681 ggcttttttg tagaaataca tcggcgcggc gtacagcgca atcaaggtcg ccgccagctc
     1741 ggttttggtc ggcgcatggc cgtaatattc ttcggcaatc gcctcggcgg aaaattcttc
     1801 ttcgccgcat acttcccaca ataaatcggt gtcgatgtcc gccgcctgtg cctgcgcgtt
     1861 ttccaaaaac gccgccatat cgccgtcaaa ctcggcaaag acattgtttg ccttcacttt
     1921 ggtgcgttta ccgtgttggg tatcgacttg gtaggtggcg tcgttttttt ggacgatggc
     1981 ggcgactttg aattggccgg actcttcgta aaaaatattc atttttcgga tttttctgtg
     2041 gaaactcaag cgggcgattt tagcagatta ccgaaaatgc cgtctgaaaa aaggttgggg
     2101 gagggttggc gcgtctttgc ggtgcttgcg ttacgttttt gacggggtac aatcgccatt
     2161 tttgaacgga ggacggaatg gggaatacaa accgtgtgcc ggagcaggca cgttatgatg
     2221 ccgaacgcag gcaggcagac gaagcattgg cgggcgtgtt tccggcagtc agtatcttcg
     2281 gcagcgcgcg cacgccgcag aatcatgcgg attatgcgtt cgccttccgt ctggcgcggc
     2341 ggctgtcgga ttcgggcatt gccgtcattt cgggcggcgg gccggggatt atggaggcgg
     2401 caaacaaggg cgcgtttgca gggaagtcgg tttcggtggg gctgaacatc gttttgccgc
     2461 acgagcagaa accgaatccg tatcaggaca tcgccttgcg gttttcccgt tttgccgaac
     2521 gcaaggcggt gtttttccgc tattcccaag catatgtcgt gatgccgggc ggcttcggga
     2581 cgctggacga attgtttgaa atcctgacct tggtacagac gggcaaagtg ccgccgcgtc
     2641 cgattgtttt ggtcggaaag gcgttttggt cgggcttggc ggagtggata aacgcgcagc
     2701 ttttggcgcg cggtctgatt tccgaagggg cagcctcttt gttcgccata tcggacgatg
     2761 aagacgaaat cgttgcgtat ctgtcggaac acgggcttca gacggcatag cgtcctgaga
     2821 gtgatgtata attgcaaaca atttaacaat ttttgatgtc tttcccgaac aggatgccga
     2881 aatgatcaac cccatcgcct cgctttcccc tttagacggc cgttatgccc aatccgttga
     2941 agcattgcgc ccgatttttt ccgaatacgg cctgatgaag gcgcgcgtca aagtcgaatt
     3001 aaactggctc aaagccctcg ccgccgagcc gaagattgcc gaagtgccgc ccttcagtgc
     3061 cgaaacgctt gccgaaatcg acacggtgat tgaaaacttt tcattggaag acgcggccgc
     3121 cgtcaaagcc atcgaagcca ccaccaatca cgatgtcaaa gccatcgaat attggctgaa
     3181 aaaacgtttt gccgaagtgc ccgaagtcgc cgccgtgagt gagttcatcc acttcgcctg
     3241 caccagcgaa gacatcaaca acctgtccca cgctttaatg ctgcaagaag cgcgtgaggc
     3301 tgttttgctg cccaaactgg cagaaatcat cggcaaactc accgctatgg cgcacgacct
     3361 tgccgccgtc ccgatgatga gccgcaccca cggccagccc gccacgccga ccactttggg
     3421 caaagaaacc gccaatgtcg tgtaccgcct gcaacgccag tttaaaaacc tgcaagcgca
     3481 agaattcctc ggcaaaatca acggcgcggt cggcaactac aacgcccata tggttgctta
     3541 tcccgatgtg gattgggaaa cccactgccg caactttgtc gaaatcagcc tcggtctgac
     3601 cttcaacccc tacaccatcc aaatcgaacc gcacgactat atggcggaat tcttccaaac
     3661 cctcagccgc atcaacacga ttctcatcga ctttaaccgc gacgtttggg gttatatttc
     3721 attgggttac ttcaaacaaa aagtcaaagc gggcgaagtc ggttcttcca ccatgccgca
     3781 caaagtcaac cccatcgact ttgaaaactc cgaaggaaac ctcggtatgt ctaacgccgt
     3841 attgggcttt ttgtccgaaa aactgccgat ttcccgctgg cagcgcgacc tgaccgacag
     3901 caccgtattg cgcaatatgg gcgtaggcgt gggctatgcc gtattgggtt tcgccgccca
     3961 cctgcgcggt ctgaacaagc tcgaacccaa ccccgccgcg cttgccgccg atttggatgc
     4021 cacttgggag ctgctcgccg agccgattca aaccgtaatg cgccgttacg gtgtcgccaa
     4081 tccttacgaa aaactgaaag acctgacgcg cggcaaaggc ggcatcacgc ccgaagtgct
     4141 gaaaggcttt atcgaatcgt tggaaattcc ggtagaagcc aaagcaaaat tgcttgagct
     4201 gactcctgca ctgtatgtgg gcaaggctga agcgttggcg aaacggattt gagcgtttac
     4261 tgaaaccgat gccgtctgaa cgcgcgttca gacggcattt ttaaaatacc ggatatacag
     4321 gggcgatatt tatgcaaact gtccgataca ggcctgaaat tgacggatta cgggctgtcg
     4381 ccgtcctttc cgtcattatt ttccacctga ataaccgttg gctgcccgga ggattcctgg
     4441 gggtggacat tttctttgtc atctcgggat tcctcattac cggcatcatt ctttctgaaa
     4501 tacagaacgg ttctttttct ttccgggatt tttatacccg caggattaag cggatttacc
     4561 ctgcctttat tgcggccgtg tcgctggctt cggtgattgc ctctcaaatc ttcctttacg
     4621 aagatttcaa ccaaatgcgg aaaaccgtgg agctttctgc ggttttcttg tccaatattt
     4681 atctggggtt tcagcagggg tatttcgatt tgagtgccga cgagaacccc gtactgcata
     4741 tctggtcttt ggcggtagag gaacagtatt acctcctgta tcctcttttg ctgatatttt
     4801 gctgcaaaaa aacaaaatcg ctacgggtgt tgtgccacat cagcatcatc ctgtttttga
     4861 ttttgactgc ctcatcgttt ttgccaagcg ggttttatac cgacatcctc aaccaaccca
     4921 atacttatta cctttcgaca ctgaggtttc ccgagctgtt ggcaggttcg ctgctggcgg
     4981 tttacgggca aacgcaaaac ggcagacggc aaacagcaaa tggaaaacgg cagttgcttt
     5041 catcactctg cttcggcgca ttgcttgcct gcctgttcgt gattgacaaa cacaatccgt
     5101 ttatcccggg aatgaccctg ctccttccct gcctgctgac ggcgctgctt atccggagta
     5161 tgcaatacgg gacacttccg acccgcatcc tgtcggcaag ccccatcgta tttgtcggca
     5221 aaatctctta ttccctatac ctgtaccatt ggatttttat tgccttcgcc cattacatta
     5281 caggcgacaa acagctcgga ctgcctgccg tatcggcggt tgccgcgttg acggccggat
     5341 tttccctgtt gagttattat ttgattgaac agccgcttag aaaacggaag atgaccttca
     5401 aaaaggcatt tttctgcctc tatctcgccc cgtccctgat acttgtcggt tacaacctgt
     5461 acgcaagggg gatattgaaa caggaacacc tccgcccgtt gcccggcgcg ccccttgctg
     5521 cagaaaatca ttttccggaa accgtcctga ccctcggcga ctcgcacgcc ggacacctgc
     5581 ggggttttct ggattatgtc ggcagccggg aagggtggaa agccaaaatc ctgtccctcg
     5641 attcggagtg tttggtttgg gtagatgaga agctggcaga caacccgtta tgtcgaaaat
     5701 accgggatga agttgaaaaa gccgaagccg ttttcattgc ccaattctat gatttgagga
     5761 tgggcggcca gcctgtgccg agatttgaag cgcaatcctt cctaataccc gggttcccag
     5821 cccgattcag ggaaaccgtc aaaaggatag ccgccgtcaa acccgtctat gtttttgcaa
     5881 acaacacatc aatcagccgt tcgcccctga gggaggaaaa attgaaaaga tttgccgcaa
     5941 accaatatct ccgccccatt caggctatgg gcgacatcgg caagagcaat caggcggtct
     6001 ttgatttgat taaagatatt cccaatgtgc attgggtgga cgcacaaaaa tacctgccca
     6061 aaaacacggt cgaaatatac ggccgctatc tttacggcga ccaagaccac ctgacctatt
     6121 tcggttctta ttatatgggg cgggaattcc acaaacacga acgcctgctt aaatcttccc
     6181 acggcggcgc attgcagtag cctgccttct tgtcggatat tgcctttggc agcctatgcc
     6241 gctgtttgcc gttcggggcg gc
//
LOCUS       DAWGRZ010000123         6237 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000123 DAWGRZ010000000
VERSION     DAWGRZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.123"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            175..1233
                     /gene="alr"
                     /locus_tag="WHT44_001570"
     CDS             175..1233
                     /gene="alr"
                     /locus_tag="WHT44_001570"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ2533736.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLIRHWKKTVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGGDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(1297..2652)
                     /locus_tag="WHT44_001571"
     CDS             complement(1297..2652)
                     /locus_tag="WHT44_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ2533737.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISSDINVLNQ
                     NIYNKITTVGKDLVTTAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETVKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(2667..2939)
                     /locus_tag="WHT44_001572"
     CDS             complement(2667..2939)
                     /locus_tag="WHT44_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ2533738.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENQINILNISQQLMDDFFT
                     MIILVDTSKCSKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(3065..3802)
                     /locus_tag="WHT44_001573"
     CDS             complement(3065..3802)
                     /locus_tag="WHT44_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ2533739.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVVAGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHQRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(4083..4982)
                     /gene="prmB"
                     /locus_tag="WHT44_001574"
     CDS             complement(4083..4982)
                     /gene="prmB"
                     /locus_tag="WHT44_001574"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ2533740.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            5127..5720
                     /locus_tag="WHT44_001575"
     CDS             5127..5720
                     /locus_tag="WHT44_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533741.1"
                     /translation="MADGMGNLIGWEKTGLVVGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     KNSGHELKVTWQGVKHAASKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDDHKHYDHFILKDE"
     gene            5832..>6237
                     /locus_tag="WHT44_001576"
     CDS             5832..>6237
                     /locus_tag="WHT44_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Caudovirus prohead protease"
                     /protein_id="HEZ2533742.1"
                     /translation="MTKLYAEIAKMETQDDDTVKVWGYASSEEIDSDGEVIAAAAMKA
                     AIPDYMKFGAGREMHGSNAAGTAIEINVEDDGRTFFVAHIVDPVAVTKVKTGVYKGFS
                     IGGSVTAHDELNKSQITGLKLTEISLVDRPANP"
BASE COUNT         1693 a         1568 c         1452 g         1524 t
ORIGIN      
        1 tttgagaaag cgattatatc aggaaaagca aaccgccttc ctacctgaaa actgctgctt
       61 cggcttgaag acacaaggtt ctttaatatt ttaaaagcct tgccgttgga ttataatccc
      121 ccaaccgatt tcttaatttt gctaataaac acttgcttgg taaggaatga atttatgcgc
      181 cctttgaacg tgcagatcag gttgggcaac cttaggcaca attatcggat tttgaaggaa
      241 atgcacggag gcaaactgtt ggcggtagtg aaggccgacg catacggaca cggtgcggtc
      301 agatgtgctt tcgcgctggc agacttggca gacggctttg ccgtggcgac aatcgatgaa
      361 ggaatcaggc tgcgggagag cggcattacc catccgattg tccttttgga aggcgtattt
      421 gaagcatcgg aatacgaagc ggtcgaacaa tactcgcttt ggccggcagt cggaaaccaa
      481 tggcagcttg aggctttgct gatccgccat tggaaaaaaa ccgtcaaagt ctggttgaaa
      541 atggattcgg ggatgcaccg taccggtttt ttccctcatg attacgcttc ggcatatgcg
      601 gcattgaagc agtcggaata tgtggacagt attgtcaaat tctcgcattt ctcctgtgcg
      661 gacgaacccg aaagcggtat gacggaaata cagatggaag cattcgattt gggtacggaa
      721 gggctggaag gcgaagaaag ccttgccaac tccgccgcta ttttgaatgt tcccgaagca
      781 cgcagggact gggggcgcgc cggtctggcg ttatacggca tttccccgtt cggaggaggc
      841 gatgacaggc tgaagcccgt gatgaggctt tcaacccgta ttttcggcga acgcgtttta
      901 cagccgcact cccctatcgg ttatggcgca acattttata ccagcaaatc tacgcgcgtc
      961 ggcctgattg cctgcggtta tgcggacggt tatccgcgcc gcgccccaag caattccccc
     1021 gtcgctgtcg acggcaaatt gacccgggtc atcggcaggg tctctatgga tatgatgacc
     1081 atcgagctgg atgcttcgca agaaggtttg ggacacgagg tcgaactgtg gggcgatacg
     1141 gtcaacatca ataccgttgc cgaagcggcc ggaaccatcc cttacgaatt gatgtgcaat
     1201 atcaaacgtg caaaattcac ttatatcgag taatcaagtc caaacgaaaa tgccgtctga
     1261 agcctttcag acggcatttc cccatcaaaa ccgcaatcag tttttcatcg attgaaccgg
     1321 agccggaatt ctgccgcctc ggttgacgaa tacttcgcac gaaccttctt tgaccggcat
     1381 cacaggcgcg tagcccaaca agccgccgaa ctcgacgctg tcgccgacgg ttttaccggt
     1441 taccggaata atgcgcacgg cagtggtttt gctgttgatc atgccgatgg cggcttcgtc
     1501 ggcaatgatg ccggaaatgg tgtgcgcggg cgtgtcgccg ggaacggcaa tcatatccaa
     1561 gccgaccgaa caaacggcgg tcatggcttc gagtttgtcc agcgtcagca cgcctgcttc
     1621 ggcggcggca atcatacctt cgtcttcgga aacggggata aacgcgccac tcaaaccccc
     1681 gaccgcgctg gaagccatca tgccgccttt tttcacggca tcgttcagca atgccaaagc
     1741 tgctgttgtg ccgtgcgtac cgcagacgct caagcccatt tcttcaagaa tgcgtgccac
     1801 tgagtcgccg acggcggggg tcggcgccag cgacaagtcg agaataccaa acgggatatt
     1861 cagcattttt gaggcttcgc ggccgatgag ttcgcccacg cgggtaattt tgaaagcagt
     1921 tttcttcact acttccgcaa cttcggtcaa tgtcgttgca tctgaatttt ccaacgcggc
     1981 ttttacgaca cctgggccgg atacgccgac attgataacg gcatccgctt cgcccgaacc
     2041 atgaaacgcg cccgccataa acgggttgtc ttccaccgcg ttgcagaaca cgacaatttt
     2101 agcgcagccg aaaccttcgg gcgtgatttc cgccgtgcgt ttgacggttt cgcccgccag
     2161 cttgaccgca tccatattga taccggcacg cgtactgccg atattgatgg agctgcacac
     2221 aatatcggta gtcttcatcg cttcgggaat ggagcggatt aacacctcat ccgaaggcga
     2281 catccctttt tgcaccaacg cggaaaaacc gccgataaaa gacacaccga tggctttggc
     2341 agctttatcc aaagtttgcg ccacgctgac gtaagaatca gcatgggtgg ccgccgcgat
     2401 ttgggcaatc ggcgtaacgg aaatgcgctg attcacaatc ggtacgccgt atttggcaga
     2461 cagatatttt gccgtagtga ccaagtcttt gccgactgtg gtaattttat tgtaaatatt
     2521 ttggttcaac acattgatat cgctgctgat gcagtcgtgc aaatcaatgc cgatggtaat
     2581 ggtgcggaca tcaaaattct ggtcggcaac cattttgacg gtttctaaaa tttcgccgga
     2641 ttggatactc atcacattcc tccaactcaa atgcggtgca tcgcttggaa gatttcttcg
     2701 ttttgcatac ggatatcaag cgcgagtttt ttgctctctt ccgcaaacaa atccaaaacc
     2761 tcttgacgcg atttgctgca ttttgaagtg tccaccaaga taatcatagt aaaaaaatcg
     2821 tccatcagct gttggctgat gttgagaata ttgatttggt tttccgccaa aattttggaa
     2881 acatcgtaca cgatgccgac gcggtcttta ccgatgacgg tgatgactga attgttcaca
     2941 ggcttactcc ttgcagatat ccgttaaagt ccgaaattat accaccgttg gattttgaag
     3001 aaatattgtc aacaatatat acatacaaaa tgccgtctga aactatttca gacagcatca
     3061 agattcaggg ttcgattaaa taaccatcct tatcccactg ggttttcctg accaacttgt
     3121 catcctgata aacagcttcg ctctttttag aaccatcttc ataccactcc aaaaccaccc
     3181 cgttgcgttg atggtggcgg atagacagtt ccgagagtaa tcggccgctt tcatcccaag
     3241 tcagaatttt ggcaggctca tcgttgacca taaccatttc cgtcttgata ctgccgtcgg
     3301 cataccattg cttccatacg ccgtttgcct tattttgctt aaactggatt tcgctttcct
     3361 tgccgccgtt acggtaatag cggtatcccg taccctcact caagccattt ttataaggca
     3421 taacggcaga ttttttaccg ttcggatacc agttgaccca ctccccgtcc ggcttaccct
     3481 tgctgaagcc ccccgccatt tttttctgac cattaaaatg ccacaaaatc aacataccgt
     3541 tttgcagggt aggcacaaaa gatttgattt gcgttgaagc aacgatataa ggttcagaat
     3601 atttcttcat cgacggataa taaaaatcct gcgcgtgcgc aatacccgcc accacactat
     3661 attgcctgat ataagcggca gaagacatcg tcgccgtcag ctttccgttc tgattaaaat
     3721 aaacagaata ggtctgcgcc ggcaaagcgg ccgaaaaacc caacaggaca gttgaaaata
     3781 caatccgaga taattttttc attgcaatag cgatataaaa acaaggctgt gttttagtaa
     3841 tctgttgatt tcaattattt gcaagggaaa agacaattat tttccggtta ggaataaacc
     3901 tattctattg aatatattga agccaagtac gcctatcaac actatattaa aacactgcca
     3961 aaaacaatta acttataaac aatatggtaa ggatttctct gccaagcatc aaacccgaga
     4021 caacgtatcg taaaaatgcc gtctgaaaac aaatcgtctt cagacggcat ttccccttca
     4081 actcactctt cacccaataa ctgctcgcgc gtcaagagga aaacaaaacc gtcgcccccg
     4141 ctggtttcca accaagtaaa aggcaactcc ggatacgctg cttccaatac atccctgtta
     4201 tgcccgattt ccaccagcaa tacacctttg ggattcagaa actttgccgc attcagaaga
     4261 atctgcctgg tggcatccaa cccgtccgcc ccgctgccca atgccaattc cggttcgtgc
     4321 aaatactctt caggcaataa ctcaaccgat tccgcatcca cataaggagg attggaaaca
     4381 atcaaatcat aagtgccttc caatccttca aacaaatccg tatgaataag ccggatgcgt
     4441 tcttccaaac cataatcttc gacattaatc cctgccactt ccaaagcatc caagctcaca
     4501 tcaaccgcat caatttgggc atcaggataa tgatgcgcca tctgaatggc aaggcaaccg
     4561 cttccggtgc aaagatccaa agcattatgc accaactcat cgtattctat ccaaggacga
     4621 agtccgtcac ccaacaattc ataaataaaa gaacgaggta tgattacgcg ctcatccaca
     4681 tagaaatcaa actctccctg ccatgcctgg tgtgtcaaat aagcggctgg aatgtgttcg
     4741 acagcacgac gctcaataac cgccagcact tcctcttttt cagcttccaa gagttttgca
     4801 tcaagatatg gggcaagcat atccaaaggc aaattcaaag tatgcagaat caaataagct
     4861 gcttcatcat gcgcattatc tgttccatga ccaaaaaaga gccctgcctc attaaaacgg
     4921 ctgactgcaa aacgtaaaat atcgcggata gtcgtcaatt cttgtgctgc ctgattaaac
     4981 ataatatgaa ccattctgcg tatagatact tttaattata acagaaacaa caagcaaacc
     5041 ttttcatatc gccaaataac cacccaatct acccatacaa ctacataaat gcccgcgcga
     5101 aaaccatcgc ccgaacggaa acgacaatgg ccgacggtat gggcaatctg attggctggg
     5161 aaaaaacggg gcttgttgtc ggtaagcagt ggataaccgc aaaagacgac aaggtgtccg
     5221 atgtctgcaa tgccaacggc gagatgggcg taatcgggct ttacgagcct ttctcacacg
     5281 gcgcattgac gatacccggt catccgaact gccgatgcga ggttgtttcc gtatcgggtg
     5341 gcgaattggg ggaatttgcc gaaaaaaagg agcttcgtaa agcggctatg cagtatgcgc
     5401 gggataactt tatcggcaaa agctatgtca ataaaaacag cgggcatgaa ctgaaggtaa
     5461 cttggcaagg tgtgaaacac gctgcgtcaa aggcaaatca ggcggaatta tccatcatga
     5521 caaaacttga tgacttattg cgctacgcaa aatatgaggg ttcttattcg gataggaaag
     5581 gtcatcctaa tattattgca gcacataagt atcgtgccgt tgccaaggtt gggaatgagt
     5641 ctttaaatat cggtgtgatt gtaagggaat ttccagacga ccataaacat tacgaccatt
     5701 tcatcttgaa ggatgaataa agcccttttg cagtgtcgtt ctggagcgga tagcgttaag
     5761 gcaagtacac ttccagcctt gaaaaagggc tttaaattca gcatgccatt tatacaggca
     5821 ggagtaaacc catgacaaag ttatacgcag aaatcgccaa gatggagacg caggacgacg
     5881 acacggtcaa ggtttggggt tacgcttcaa gcgaggaaat cgattcggac ggcgaagtca
     5941 tcgcggcggc agctatgaag gcggcgattc ccgattatat gaagtttggc gcggggcgcg
     6001 agatgcacgg ctcaaacgct gcgggaacgg caattgaaat caacgtggaa gatgacggca
     6061 gaaccttttt cgtggcgcat atcgtcgatc ccgttgccgt gacgaaggtc aaaacaggcg
     6121 tttacaaggg cttttccatc ggcggcagcg ttaccgccca cgatgagttg aacaagtcgc
     6181 aaatcacggg tttgaagctg acggaaatca gcttggttga ccgacccgcc aatcccg
//
LOCUS       DAWGRZ010000124         6185 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000124 DAWGRZ010000000
VERSION     DAWGRZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.124"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(263..1306)
                     /locus_tag="WHT44_001577"
     CDS             complement(263..1306)
                     /locus_tag="WHT44_001577"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ2533743.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPDLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEAGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGSDPETVLRDYYRDSPFVDI
                     LPTGSAPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     EETHGLDAIPLLP"
     gene            complement(1303..1584)
                     /locus_tag="WHT44_001578"
                     /pseudo
     CDS             complement(1303..1584)
                     /locus_tag="WHT44_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1839..2993
                     /locus_tag="WHT44_001579"
     CDS             1839..2993
                     /locus_tag="WHT44_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ2533744.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLESASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDPRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQGKYEEVRKLIYQLIELDFLEIL"
     gene            3222..3575
                     /locus_tag="WHT44_001580"
     CDS             3222..3575
                     /locus_tag="WHT44_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533745.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDLVAI
                     PAAAAAVYYLPKNAYGAAGANGVYNVTRNWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            3636..4352
                     /locus_tag="WHT44_001581"
     CDS             3636..4352
                     /locus_tag="WHT44_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ2533746.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHGNETRSLKATVERLENQELH
                     ISQQIDGQKRRIRLAEEMLQKYRFLSANDAVPKQEMMNVKAELLEQKAKLDAYRREEV
                     GLLQEIRTQNLTLASLPQAA"
     gene            4417..4860
                     /locus_tag="WHT44_001582"
     CDS             4417..4860
                     /locus_tag="WHT44_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ2533747.1"
                     /translation="MRSEQIIRAGRSGYIAIPNVEVGQQVDPSKLLLSIVPERTELYA
                     HLYIPSSAAGFIKPKDKVVLRYQAYPYQKFGLASGSVVSVAKTALGRQELSGLGMVSS
                     DLAKSNEPVYLVKIKPDKSTITAYGEEKPLQIGMTLEADILHEKR"
     gene            complement(5109..5216)
                     /locus_tag="WHT44_001583"
                     /pseudo
     CDS             complement(5109..5216)
                     /locus_tag="WHT44_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            5226..5528
                     /locus_tag="WHT44_001584"
     CDS             5226..5528
                     /locus_tag="WHT44_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533748.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            5812..6051
                     /locus_tag="WHT44_001585"
     CDS             5812..6051
                     /locus_tag="WHT44_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ2533749.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTNL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGEFCKGLIL"
BASE COUNT         1695 a         1359 c         1524 g         1607 t
ORIGIN      
        1 gcgggaatct agaaatgaaa aaccacggtg ttatcggcaa tgactgaaac cggagaaatg
       61 acggaatcga agcgtgtgga aatgtgtcgg aaattgctga agctggataa actagattcc
      121 cgcttttgcg ggaatgacgg tattttagat ttttgttttc gtgtgaatgg atgggtcaaa
      181 agccgtccga acccaaaaaa acgtcgcttt cagacggcat caaaaaccgc aggcacgatg
      241 cctgcggttt gctgtttgcg cttcagggga gcagggggat tgcgtccaag ccgtgtgttt
      301 cctccagtcc gaacataatg ttcatatttt ggactgcctg acccgccgcg cctttgacga
      361 ggttgtcgat gacggaaagg acgacccaca catcggattg cgccgcctgt tggatgctga
      421 tgcggcagag gtttgcgccg cgcacgctgc gggtttcggg ggcggaaccg gtcggcagga
      481 tgtccacgaa cgggctgtcg cggtagtagt cgcgcaggac ggtttcgggg tcgctgccgt
      541 ctgaaaggtg gaggtaaacg gtggcgtgca taccgcgtat cattggcgcg aggtgcggcg
      601 tgaacacgaa tccttcggcg atgccgtcct gaagcccggc gatggtctgc ctgatttcgg
      661 gcaggtggcg gtgtccggct atgccgtagg ctttgaagtt gtcgccggct tcgcacaaca
      721 gcgaaccgac attgcctttc ctgcccgcgc cggacacacc ggatttgcag tcggcaatca
      781 gcggcatacc gggcttcaga cggcattgcc gcaacagcgg cacgagcggt agggatacgc
      841 aggtcgggta gcagccgggg ttggcgacga ggcgcgcctg tgcgacggct tcgcggttga
      901 gttcgctcaa tccgtacacg gcttgggaaa cgaggtcggg ggcggcgtgg gtcatgccgt
      961 accagtgttc ccaggtcgga atgtcccgta tgcggaagtc ggcggaaagg tcgatgacgc
     1021 gcacgccctg ttcaatcagg cgcggcgcgt ctttcatggc gatgccgttg ggcgtggcga
     1081 agaagacgat gtcgcattgt tccaaacctg cctcgtcggg cgtttggaag gcgaggccgt
     1141 acacgccgcg caaactcgga aagtaatcgg caactgcggt tcccgcttcg ctgcggctgg
     1201 ttacggcggc gacttcgaca tcgggatggg cggcaagcag gcgcagcagt tccacgccgg
     1261 tgtagcccgt cgcgccgaca atgccgactt tgattttttt actcatggtt tttcctttgt
     1321 gtggttggcg ggtatgccgt ccgaacgctg tccgacgggg ttttgattgg cggcggaagg
     1381 acggtacaca aagcgtttga aaagcgtgtc taatttaatg gcatccgcct ctgtttttaa
     1441 aacctagccc tgccgtccgg aataaacata accgtaggcg cgaaagccgg caggatattt
     1501 cggtgcctgc cccaaaccca aaccgtatgc ggcgaaagcg gtggtcgtgg agaaggaaag
     1561 caggaggagg acgagggttt tcataaggtt ccaaaccgaa cggggcgatg aagcccgatt
     1621 atagcaaaag gtcgtctgaa aaccgttttc agacgacctt ttatttaatt acaataaaaa
     1681 tgttaacaac aaaaaacaaa ccgctttttt ccgtttggag acaaattttt aactaaaatt
     1741 gtcaacaatt ccttgacgca cacacaaaaa aaaactttaa tatttgtaaa tatatgtaac
     1801 attataaatt ttaaatattt tctgagtaag gggaagtaat gagcatccat ctcgacttcg
     1861 gcattagtcc taaaacgttc cgacagactt atctgtatca aaagcccaag ctctttaaag
     1921 gagcggttcg gaatctcgaa tccgcatctt ggaaagatat caacgagata taccaacgag
     1981 cagacccaac cgcaccgctg tttcatctgc gtaaaaaagg cgcaatcgtt cctaaagaag
     2041 aatacgtcga aagtttcgac gatttgggca aaactcgcta ccgttttatt aaatccgtta
     2101 tctacgaaca tatgaagaat ggtgcgtcgt tagtctataa ccatattaac aacgagccgt
     2161 tttcagacca tatcgcccgt caagtcgccc gctttgccgg cgcacatact attgttagtg
     2221 gatatcttgc ttttggcagc gacgaatctt ataaaaacca ttgggacccc cgcgatgtgt
     2281 atgccatcca gcttttcggc aagaaacgtt ggcaacttac tgcccctgat ttccctatgc
     2341 cattgtatat gcaacagact aaagatactg atatttccat tcctgaacat atcgatatgg
     2401 atattatcct tgaagcaggt gatgtcctct acatcccacg cggttggtgg cacagaccta
     2461 tcccgctcgg ctgtgaaacc ttccacttcg ctgtcggtac ctttccaccc aacggctata
     2521 attacctcga gtggctaatg aagaaatttc ccaccataga aagtctgcgc cacagtttct
     2581 cagactggga gcaagatagg acgcgtatca acgatactgc cgcacaaatt gctgccatga
     2641 ttgccgaccc cgtcaattat gaagccttca gtgaagactt tctcggcaaa gaacgtaccg
     2701 ataccgcttt tcatctcgaa cagttcgcga atcccaacgc tactccgctt tcagacgacg
     2761 tcaggttgag attaaatgcc aataatttgg atacgttgga aaagggatat ttgattggga
     2821 atgggatgaa gataagcgta gatgagttgg ggaaaaaagt gttagaacac atcggtaaga
     2881 atgaaccgtt attgttgaaa aatctactgg ttaacttcaa tcagggaaaa tatgaagaag
     2941 ttaggaagtt gatttatcag ttgatagagt tagattttct ggaaattttg tgagggattc
     3001 tatgaaaaac tggaagcaat ttacattttt cgtaatatta gtaatagctt gttatcaagt
     3061 gctatatttt ttatcagata tgtttcttct cgactacatt aacaaatata gttggaattt
     3121 gaattttatt cagggtactt tgaatttttt ttcaatatat ctgccatatg tttttgttag
     3181 ccgaattttt aggaatacca accaagaaaa ggagtataaa aatgattgaa cttcaacttc
     3241 atgaattgaa gctggtttca gggggaggtc ctgtaacaga caatatagct ggaaatgtag
     3301 ctaatgctgc cacaaccaaa ggaggtccca catgggggga tttggttgca atacctgctg
     3361 ccgcagcggc agtttattat ctgccgaaaa atgcttacgg tgctgctggt gcaaatggtg
     3421 tatacaatgt gactcgtaat tgggtaaatg atgccgttaa tgcacctcct tataacggaa
     3481 gaccaatctt tgagattgaa catggattaa ctgctcccgc aacaaaagca gataaatcag
     3541 gaaacggcta cactgacggt acagattact gctgatattt ctcatcgtcc agacaatctc
     3601 taggggtcgt ctgaaacttt cttaacttca attttatgaa tagacccaag caacccttct
     3661 tccgtcccga agtcgccgtt gcccgccaaa ccagcctgac gggtaaagtg attctgacac
     3721 gaccgttgtc attttcccta tggacgacat ttgcatcgat atctgcgtta ttgattatcc
     3781 tgtttttgat atttggtaac tatacgcgaa agacaacagt ggagggacaa attttacctg
     3841 catcgggcgt aatcagggtg tatgcaccgg atacggggac aattacagcg aaattcgtgg
     3901 aagatggaga aaaggttaag gctggcgaca agctatttgc gctttcgacc tcacgtttcg
     3961 gcgcaggagg tagcgtgcag cagcagttga aaacggaggc agttttgaag aaaacgttgg
     4021 cagaacagga actgggtcgt ctgaagctga tacacgggaa tgaaacgcgc agccttaaag
     4081 caactgtcga acgtttggaa aaccaggaac tccatatttc gcaacagata gacggtcaga
     4141 aaaggcgcat tagacttgcg gaagaaatgt tgcagaaata tcgtttccta tccgccaatg
     4201 atgcagtgcc aaaacaagaa atgatgaatg tcaaggcaga gcttttagag cagaaagcca
     4261 aacttgatgc ctaccgccga gaagaagtcg ggctgcttca ggaaatccgc acgcagaatc
     4321 tgacattggc cagcctcccc caagcggcat gagacagaac aaagctggct tgaacgcacc
     4381 attgccgata tttctcaaga agttttggat tttgaaatgc gctctgaaca aatcatccgt
     4441 gcaggacggt cgggttatat agcaataccg aacgtcgaag tcggacagca ggttgatcct
     4501 tccaaactgc tcttgagcat tgttcccgaa cgtaccgagc tatatgccca tctatatatc
     4561 cccagcagtg cagcaggctt tatcaagccg aaagacaagg ttgtcctacg ttatcaggca
     4621 tatccctatc aaaaattcgg gcttgcttcc ggcagtgtcg tatcagtagc aaaaacggca
     4681 ctgggcagac aggaattgtc gggattgggc atggtatcct ccgatttggc gaagagcaac
     4741 gaacctgttt atcttgtgaa aataaaaccc gacaaatcaa ccatcactgc atacggtgag
     4801 gaaaaaccgc tgcaaatcgg catgacgttg gaagcagaca tcctacacga gaaacggtga
     4861 ttgtacgaat gggtattgga gctgatttat agtatgtcgg gcaaactgta agacttgagg
     4921 ctgtaaagat aaagaagttg tctgaaacct tttcagacga ccagaagaca atatataaaa
     4981 acacaaactt caaaactgcc tgaaaccatc cccttttaca ggcactccgt ttgtaaaagc
     5041 aaggataggg catctgccct tttgattgtt tgattaacga tacaaggagt ttcaaaatga
     5101 gagttttata gtggattaaa tttaaaccag tacagcgttg cctcgccttg ccgtaccatt
     5161 tgtactgtct gcggcttcgt cgccttgtcc tgatttaaat ttaatccact atatgtgttc
     5221 atgaaatgac ttgggtcgga ggctcaggta atgcgcaaca aagctcatat tattgcgaaa
     5281 tttgcgaatc tgcagggctt aacgatacgg gaaatcctga taaatcttta ggattgccaa
     5341 acaatacgtt cagtaatccg cctggttggg gagctacaat cggagcttta gcaggtagcc
     5401 gcatagggat gcctgaattt ggtacgtttg cgagccatgc cattgaaaat ttcgactggt
     5461 catggtatcg acgttatagg gaaattgccg aaacgattga acgagaatat tcaggcggtt
     5521 tgccttaata gttgaggagg tcatgatgtt tgccaaacat tatcaattca tcgcactcgg
     5581 catcatgctg cttctttata tgttgattct ctatacgacc gatttttcca atctgacgta
     5641 ttggatgctg ttttttatct gttttattac aggaaaaata ttagctcgtt tgttagagaa
     5701 aagctttaaa taaaatagca gctagtcgca aaaggtcgtc tgaaaccttt tcaggcggcc
     5761 tttctaaaat acatccaact tcctaatccc tatttttcaa aaaggaaatc tatgccccat
     5821 ctgcaaaacc tgtctttggg cttaaagaaa aagctgcctg ttatcctgca aacagaaata
     5881 tcagaatgcg gcttggcatg tctggcggct gtggcgggat ttcatggttt ccatacgaat
     5941 ttacgcgcac tgcgttcaaa atactgtccg agacctttgc aaaattcccc aaaatcccct
     6001 aaatgtcttg gtgggaattt tggggaattt tgcaaaggtc tcattctata actgtaaata
     6061 cttttaaatt tatgacaaaa tagtaaatat tgctaaaata atattgatgt catgaaattt
     6121 tttcctgctc catgtctgtt ggttatcctg gctgtcatac cccttaaaac cttagctgcc
     6181 gatga
//
LOCUS       DAWGRZ010000125         6170 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000125 DAWGRZ010000000
VERSION     DAWGRZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.125"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            61..444
                     /locus_tag="WHT44_001586"
     CDS             61..444
                     /locus_tag="WHT44_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ2533750.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKLA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            737..1210
                     /locus_tag="WHT44_001587"
     CDS             737..1210
                     /locus_tag="WHT44_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ2533751.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVEGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMPAMNHGHHHGEAHQH"
     gene            1336..2439
                     /gene="mnmA"
                     /locus_tag="WHT44_001588"
     CDS             1336..2439
                     /gene="mnmA"
                     /locus_tag="WHT44_001588"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ2533752.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFKLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDDETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            2510..4180
                     /locus_tag="WHT44_001589"
     CDS             2510..4180
                     /locus_tag="WHT44_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ2533753.1"
                     /translation="MEKIWLDSYEKGVSAEIDITQYNSVSDVFRQSVEKFADQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPVALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFVIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSELRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            4292..5926
                     /locus_tag="WHT44_001590"
     CDS             4292..5926
                     /locus_tag="WHT44_001590"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ2533754.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSENIEK
                     NKGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESTDLGGTMRLGAQEVELK
                     AGSLAAKIYGSGHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
     gene            5941..6143
                     /locus_tag="WHT44_001591"
                     /pseudo
     CDS             5941..6143
                     /locus_tag="WHT44_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246312.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1621 a         1658 c         1553 g         1338 t
ORIGIN      
        1 ggcaacgccg tacaggtttt tgttaatcca ctatattgag aacattttga aaggataccg
       61 atggaacctt cctcctacgc ggcagaaaaa aaaggcaaag gcggcatcag gcgcgtcatt
      121 aacgcattcg gctattcgat agacggcatc gccgccgcct accgttacga agcggcattc
      181 cgtcaggttt tgtggctgaa cgtactgctg gtgtgcgcgg catttttttg ggtttccgaa
      241 accgccgtcc gcctgccgtt gattatcgcg tcttttgtgt cggtcattgt cgaactgttc
      301 aacaccgccg tcgaagctgc cgtcgatcat acttcgaccg aaaaacacga gctggccaaa
      361 ctcgccaaag acgcaggttc tgccgcacaa ttgttcgcga tgctgatgtt ggcggcggtt
      421 tggctgtctg ccctgttcgg gtaaaacgct tgcagcagga tttgtaatcc tttaggatta
      481 gtatgtggta tttattattc acttaatcta catcaaattt ccaggcagta tttgtatgta
      541 tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg
      601 aaccgatcca cttggtgctt cagcacctta gagaatcgtt ctctttgagc taaggcgagg
      661 caacgctgta ctggtttttg ttaatccgct ataagattaa gcacattccc cgtctgatat
      721 tataaggagc agaaagatga aaaaattatt ggcggccgtg atgatggcag gtttggcagg
      781 cgcggtttcc gccgccggag tccacgttga ggacggctgg gcgcgcacca ccgtcgaagg
      841 tatgaaaata ggcggcgcgt tcatgaaaat ccacaacgac gaagccaaac aagacttttt
      901 gctcggcgga agcagccccg ttgccgaccg cgtcgaagtg catacccaca tcaacgacaa
      961 cggcgtgatg cggatgcgcg aagtcgaagg cggcgtgcct ttggaagcga aatccgttac
     1021 cgaactcaaa cccggcagct atcatgtgat gtttatgggt ttgaaaaaac aattaaaaga
     1081 gggcgataaa attcccgtta ccctgaaatt taaaaacgcc aaagcgcaaa ccgtccaact
     1141 ggaagtcaaa atcgcgccga tgccggcaat gaaccacggt catcaccacg gcgaagcgca
     1201 tcagcactaa tctgctgaaa atgtttgaaa tgccgtctga aaaagcccgg gctttcagac
     1261 ggcattttta tgcccgcctt taaaatgtgt taaaatccgc ctttaaaaac cgccgtttcc
     1321 aaccatattc tacgcatgaa tacgacagca aacccttcca atatcatcgt cgggctctcc
     1381 ggcggtgtcg attcttccgt aaccgccgcc ctgctcaagc agcagggtta tcaagtgcgc
     1441 ggtgtgttca tgcagaactg ggaagacgac gacaacgacg aatattgcag catcaaacag
     1501 gattcgttcg atgccatcgc cgttgccgat attatcggca tcgatatcga catcgttaat
     1561 ttcgccgcgc aatataaaga caaagtcttt gcttattttc ttcaggaata cagtgcgggg
     1621 cgcacgccga atccggatgt gttgtgcaac gccgaaatca aattcaaatg ctttttggac
     1681 tacgccgtag ggcagggcgc ggataccatc gccaccggcc actatgcgcg caaagaagtt
     1741 cgcaacggcg tgcattacct gctcaaaggt ttggatcgaa acaaagacca aagctatttc
     1801 ctctaccgcc tcaagccttt ccaactcgag cgcgcgattt ttccgttggg cggtttggaa
     1861 aaacccgaag tgcgccgcct tgccgccgag ttcaaactgc cgaccgccgc taaaaaagac
     1921 agcacgggca tctgtttcat cggcgaacgc ccgtttcgcg agtttctgca gaaatacctg
     1981 ccgaccgaca atggtaaaat ggttacgccc gaagggaaaa ccgtcggcga acacgtcggg
     2041 ctgatgttct acacgctcgg acagcgcaaa ggtttgggca ttggcggcgc gggcgaaccg
     2101 tggtttgttg cggctaaaga tttgacgaaa aacgaactga ttgtcgtgca aggacacgac
     2161 catccgctgc tttatacccg cagccttgtg atgaacgatt tgagtttcac gctgcccgaa
     2221 cgcccgaagg caggacgcta tacctgcaaa acgcgttacc gtatggcgga cgcgccttgt
     2281 gagttgcgct atttggatga tgaaaccgcc gagctggtgt ttgacgaacc gcaatgggcg
     2341 gttacgccgg gtcagtccgc cgtgctgtac gacggcgata tctgtttggg cggcggtatc
     2401 atccaaacga ccgacaaacc cgtcatcatc acacgataaa ggtaatgccg tctgaaacgg
     2461 ttttcagacg gcattgttcc gctcaattcc actttaaaga ccgataccta tggaaaaaat
     2521 ctggttagac agctacgaga agggcgtcag tgccgaaatc gacatcacgc aatacaattc
     2581 cgtcagcgac gtattccgcc aaagcgtgga aaaatttgcc gaccaacctg cttttcaaaa
     2641 catgggtaag acgctgactt atgccgaaac cggcaaactg gcaacagatt ttgcttctta
     2701 tctgcaaaac gtccttaaat tgcctcgagg agagcgcgtg gcaataatgt tgcccaatgt
     2761 actgcaatat ccggttgccc ttttcggcat tttgcaggca ggtttggtag cggtgaacac
     2821 caatccgctc tataccccgc gcgagctgga gcatcagttg aaagacagcg gcgcgaccgc
     2881 catcatcgtt ttggaaaatt tcgccaacac gctggagctg gtgctgccgc gcacgcagat
     2941 caaacacgtc atcgtagcct ccgtcggcga aatgttcggg ctgcttaaag gttcgctgat
     3001 caatttcgtc atccggaaaa tcaagaaaat ggtgcccgaa taccgtattc gggaaaccgt
     3061 ttcctttcag acggcattga aagaaggggc gaagcacgtt ttccaacctg tcgcattaaa
     3121 ccgcgaagat accgcgctgt tgcaatacac gggcggcacg acaggtgttg ccaaaggggc
     3181 ggtgctgagt cacggcaaca tctgcgccaa tatgcttcag gcaaaagaat ggattaaaaa
     3241 ccagttgcgc gagggcaaag aaaccgttat cgccgccttg ccgctgtacc atatttttgc
     3301 cttaaccgtg aatctgatga tttttgccaa tgccggctcg aaaatcatcc tgattaccaa
     3361 cccgcgcgat atgaaaggct ttatcggaga actgaaaaaa cagcgggtta atgtatttat
     3421 cggcgtgaac acgctgttta acgcgatggt taaccgtcct gattttgccg aagtcgattt
     3481 ttcagaattg cggctgactt tgggcggcgg tatggcgacc caaaaagccg ttgccgaaaa
     3541 atggaaaaaa atcaccggca cgcccatcgt cgaagcctac ggtctgaccg aagccagccc
     3601 cggcgtgtgc tgcaacccct taaacatcga atcatacagc ggcagcatcg gtttgcccgt
     3661 ctcgtccacc gaagtcgaac tgcgcgacgc aaacggcaaa gaagtccccg tcgggcagcc
     3721 gggcgaattg tgggtaaaag gccctcaagt gatgcaaggc tactggaacc gccccgaaga
     3781 aaccgccaaa gccatagacg cgcgcggctt cttggaaacc ggcgatattg ccgtcatgga
     3841 cgaaaaaggc tggttgaagc tggtcgatcg taagaaagac ctcgtcgttg tttccggatt
     3901 caatgtttat ccgaacgaaa tcgaggaagt catcgcgcat cacggcaaag tcatggaagt
     3961 cgcctgtatc ggcgttcccg acgaaaaaac cggcgaggca ctcaaagtat tcgtcgtcaa
     4021 aaaagacccg tctttgacca aagaagaact tatcgctttt tgccgcaccg aattaaccgc
     4081 atataaagta ccgaaaaata tcgaattccg cgacgagttg cccaagtcca acgtcggcaa
     4141 aatcctgcgc cgcgagttgc ggcaaagtac cgggaaataa agaaaagatg ccgtctgaaa
     4201 acagccgtcc atcgttcaga cggcatccgt ccgtttgcca gaaccgcgcg cttcacgtta
     4261 aaatcacgca ttccaatacg ggtatttcat tatgaccaaa ttcattttcg tcaccggcgg
     4321 cgttgtctcc tcactgggta aaggtatcgc cgccgcttct attgccgcca tcctcgaatc
     4381 gcgcggcttg aacgttacca tgctcaagct cgatccttat atcaacgtcg atcccggcac
     4441 gatgagcccg ttccaacacg gcgaagtgtt cgtaaccgac gacggcgcgg aaaccgacct
     4501 cgacttggga cactacgaac gctttatcga ttccacgatg acccgccgca acagcttcag
     4561 cacgggacag gtgtacgaaa acgttatcgc caaagaacga cggggcgact acctcggcgg
     4621 tacggttcaa gtcattccgc acattaccga cgaaatcaaa cgacgcatcc acgaaggcgc
     4681 ggcaggttac gatgtggcga ttgtcgaaat tggcggcacg gtcggcgaca tcgaatcgct
     4741 gccgtttttg gaagccatcc gccagatgcg aagccagttg gggcgcaaca acaccttgtt
     4801 tgcccacttg agctacgtcc cctacatcgc cgccgcaggc gaaatcaaaa ccaagccgac
     4861 ccagcacacc gttaaagaaa tgttgagcat cggtttgcaa cccgacatcc tgatttgccg
     4921 tatggacagg acaatgcctg cggacgaacg ccgcaaaatc gccttgttct gcaacgtgga
     4981 agaacgcgcg attgtcggca gctacgatgt ggacagcatc tacgaatgcc ccgaaatgct
     5041 gcacgaccaa ggcatcgaca acatcattac cgagcagttg cagcttaatg tgcagcaggc
     5101 ggatttgacc gcgtggaaaa aaatcgtcca cgccatccaa aacccgaaac acaccgtcaa
     5161 aatcgccatg gtcggcaaat acgtcgattt gaccgagtcc tacaaatcgc tgatcgaagc
     5221 cttgaaacac gcaggcattc acaccgaaac cgacgtgcag attacctttg tcgacagcga
     5281 aaacatcgag aaaaacaagg gcgacgtttc catgcttaaa gacatggacg ccatcctcgt
     5341 tcccggcggt ttcggttcgc gcggtgtgga aggcaaaatc gccgccgtgc gctacgcccg
     5401 cgaaaacaac gtgccatact tgggcatctg cctcggtatg cagattgcgc tgatcgaata
     5461 cgcccgcgac gtggcaggct tgaaaggcgc gaattccacc gagttcgacc tcaaatgcgc
     5521 cgcgccagtc gttgccctga ttgacgaatg gcaaactgcc gacggcagcg tcgaaacccg
     5581 cgacgaatcc accgatttgg gcggcacgat gcgtttgggc gcgcaagaag tcgaattgaa
     5641 agcaggcagc ctcgccgcca aaatctacgg cagcggacat atccgcgaac gccaccgcca
     5701 ccgctacgaa gtcaacaaca actacgttcc tacactggaa caggcaggtt tggtcatcgg
     5761 cggtgtatct gccggacgcg aacgcttggt cgaaaccatc gaactgccga accatccttg
     5821 gttcttcgcc tgccagttcc accccgagtt tacctccaat ccgcgcaaag ggcatccttt
     5881 gttcaccgcg tttgtcaaag ctgcgttgaa caataaaaaa gcctgataaa gcgttacttg
     5941 atgatcaaaa tgccgtctga aagcctgaat gaagactttc agacggcatt ttcgcaaatc
     6001 ggggattata aaagccatca atttaatttt tatggtttta tctaatattt accaactgaa
     6061 tattgttaag cggggcgtga aactcaagtt gagacctttg caaaattccc caaaatcccc
     6121 taaattccca ccaagacatt taggagattt ctcatgagca ccttcttcca
//
LOCUS       DAWGRZ010000126         6138 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000126 DAWGRZ010000000
VERSION     DAWGRZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.126"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            143..1540
                     /gene="aspA"
                     /locus_tag="WHT44_001592"
     CDS             143..1540
                     /gene="aspA"
                     /locus_tag="WHT44_001592"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ2533755.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGKLGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLVVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTTGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAINLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1612..2487)
                     /locus_tag="WHT44_001593"
     CDS             complement(1612..2487)
                     /locus_tag="WHT44_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ2533756.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWWLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGIFLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWGEVKVLFGI"
     gene            complement(2489..3211)
                     /locus_tag="WHT44_001594"
     CDS             complement(2489..3211)
                     /locus_tag="WHT44_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ2533757.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPKAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            complement(3234..3713)
                     /gene="ybaK"
                     /locus_tag="WHT44_001595"
     CDS             complement(3234..3713)
                     /gene="ybaK"
                     /locus_tag="WHT44_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ2533758.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPVQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3788..4150
                     /locus_tag="WHT44_001596"
     CDS             3788..4150
                     /locus_tag="WHT44_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ2533759.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            4185..5048
                     /locus_tag="WHT44_001597"
     CDS             4185..5048
                     /locus_tag="WHT44_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ2533760.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINHLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     EASAEPLAAQEPSLAFTAECGSDGICSVVLPHRGILHIAPKNALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEEAGIVLDSVQDSENFTALTVIKFSAAQKTLTVPNGGIFKA
                     VHLFFKTENAE"
     gene            5141..6100
                     /gene="ttcA"
                     /locus_tag="WHT44_001598"
     CDS             5141..6100
                     /gene="ttcA"
                     /locus_tag="WHT44_001598"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ2533761.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPDDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQSLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGS"
BASE COUNT         1565 a         1779 c         1482 g         1312 t
ORIGIN      
        1 acccgaaaac aacgtttccc gttttcagac ggcatacgat atggacaatt tcatccggcc
       61 ggtatagtgt tcgtcctgcg gcggcatctt tccgatggcg cattttttca atattaatta
      121 tccgacttac aaaggtaaaa acatgactgt ccgtatcgaa cacgatttat tgggcgaccg
      181 cgagattccc gccgaagtat attggggcat ccacaccctg cgcgccattg aaaactttaa
      241 aatctccaca caaaaaattt ctgacgtgcc gcagtttgtc cgcagtatgg tgatggtgaa
      301 aaaggcgacg gcacaggcaa acggtaagtt gggcgcggtc aagcccgaaa tcgcagccgc
      361 cattgaaaaa gcctgcgacg aagttctgct gaacaaccgc tgcctcgacc aattcccctc
      421 cgacgtgtat cagggcggtg cgggtacttc ggtcaatatg aacaccaacg aagtcatcgc
      481 caacctcgca ttggaagcct tgggctatga gaaaggccgc tacgacatcg tcaatccgat
      541 ggaccatgtc aacgccagcc aatcgaccaa cgatgcctat cccacgggct tccgccttgc
      601 cgtgtattac agtatcggcg aattgctcga caaactggtc gttctgaaaa acgcctttgc
      661 cgccaaagcc gaagaattta aagacgtttt gaaaatgggc cgcacccagc ttcaagatgc
      721 cgtgccaatg actacaggac aagaattcca atctttccaa gtgttgttgg aagaagaaat
      781 cctcaacctc gaccgcacgc gccaactgct tttagaagtc aatttgggcg caacggcaat
      841 cggcacgggc gtgaacacgc ccaaaggcta cgccgaactg gtggtcaaaa aactctccga
      901 agtcagcggc ttgccttgca aactgaccga aaacctgatt gaggcgacct ccgactgcgg
      961 cgcatatgtg atggtacacg gcgcattgaa gcgcacggcg gtcaaactct ccaaaatctg
     1021 caacgacttg cgcctgctct cttccggccc gcgtgccggc ttgaaagaaa tcaacctgcc
     1081 cgaattacag gccggttctt ccattatgcc tgccaaagtc aatcccgtga ttcccgaagt
     1141 ggtcaaccaa gtctgcttca aagtcatcgg caacgatacg acgattacct tcgccgccga
     1201 agccgggcaa ctgcaattaa acgtgatgga gccggtcatc gcccaatgta tgtttgaaac
     1261 catttccctc ttgggcaatg cagcaatcaa cctgtccgac aaatgcgtca aaggcattac
     1321 ggtcaaccgc gaaatatgcg agcgttacgt tttcaactcc atcgggctgg ttacttatct
     1381 gaatccgtat atcggacacc acaacggcga tttggtcggc aaaatctgcg cccaaaccgg
     1441 caaaggcgtg cgcgaggttg tcttggaacg cgggctgttg agcgaagaag aactcaaccg
     1501 catcctctct cctgaaaacc tgatgaaccc ccatctgtaa cgttgcagac aaatgccgtc
     1561 tgaagcaggg atgctgcttc agacggcatt ttgttatatc aatcagccgg attatatgcc
     1621 gaacagtacc ttgacttccc cccaataaaa caggcaggcg atagaacaca gtgccaacac
     1681 gattcccgcc gtattgattg tgttgatttt ttccttaaag aacaatgcgc ccgaaagcgt
     1741 acccaaaaca atcacgccga tattcatacc ggcaaaaacc aaggtcggat tatccttcat
     1801 catttggtgc gcggtgatgt aggttacgat attcataaaa ttcaaaccgc ccaagaatat
     1861 gccgccaacc acactctcaa cacgccatct gaccgatttg gcaaacaggc aggcaaacat
     1921 cagcacaccc gccagcacaa atgcaaccag caggttgccc gcaaatgccg ttccgctttt
     1981 ggcaagctgt ttgaacagga tatcgataat gccgtaacct gcccacacgc ccagcagcaa
     2041 tgccgcctgc cgccacgcgc ttcctgattt tttgccaccg ctgtgtttcc aaagcaggca
     2101 gaacagtgcg gcaaatgcga ggcacagccc gattagtctg ccttcgctga gtttttcgcc
     2161 aaacagcgtc aaggcggcaa caatcggcaa aaacagcgac aaacgctgcg ccgcgtcgga
     2221 tttgacgata ccggcggatt ctacggattt gcccataatg acaaatacgg acggcagcag
     2281 cacgcccaat gcggcaaaca gccaccatgt cggcaaaaat gcgccgatat tgccgacatc
     2341 cggcttcaat accagcaggg tcagtatgac cgcgaccaca taattgacgg cgaccgcctg
     2401 cgcgatgtcg atttttttct tccttgccat tttcagcaaa atggaaaccg acacgctgaa
     2461 cacgatgctg attaacaggt aagccatact atatccattt ctcgggtgca accgccctgc
     2521 cgccgttgaa gcggataaag ccggcgatga tgcggtagaa ataccagacg ttcgttgcaa
     2581 taataatcag cacgccgata cccaaaaggg cagtcagcgc acccaaaata taaagccaaa
     2641 atgtgcgcca aaaggtttta atcaggtatt cggtatgtgc ggcatagaca atgctgtcca
     2701 aactgttccg tttcatatag gcgagaatca cgccgactat gggcatatag gcgaaaatca
     2761 cgccgactat gggcatgaac agcatgattg cacccaaacc gaacaggatg taggcggcga
     2821 ggacatagtt gcgcccgctt ccgccgccgg acggttcgta agtatggcac gaacgtgatg
     2881 cctgccgttc aaacgcctgt ctgaacgcct gttcgcggcg catctgttcg cggtaaaact
     2941 gctccctgcg cgcctgttcg cggcggaatg cctcttcctg ccgcccgcgt tcctcatgcc
     3001 gtctgaagga cgcgtcgtat tgcatccgtt tcgacaggtc ggaaagcgta tcgtaagccc
     3061 gttggatttc tttgaacttt tcttccgcct tcggattgcc cggattgcgg tcgggatgat
     3121 atttcatcgc cagcttgcgg taggcgcgtt tgatttcgtc cgctcccgcc tgcggcgaca
     3181 cgcccaatac ggcatacaaa tctttgtcca tattgttcct ttatactttc ccgtcaaacc
     3241 gccgcctgta ttgttttcgg gttcaaaata tttaaatctc cgggacggat gccgataatg
     3301 aacccgcgtt ttccgccgtt gatatagatg gtttccaaat ccatcaccga ctgttcgacg
     3361 taaatatcca actttgtccg gatgccgaac ggcgttgtgc cgccgaccag atagcccgtc
     3421 cacttgtttg cctgtacggg cgtggcgggt tcaatgtgtt tcgcacccaa atgccgcgcc
     3481 agattgcggg ttgaaatctg cttgtcgccg tgcatcaaga caatcagccc cttaccgttt
     3541 tcatcttgca aaacaatggt tttaatgacc aagtgttcgt ctttgccgaa tagtcgggca
     3601 aactgcgccg tgccgccgtg ttcctcatag gcataggtaa aaggttcaaa ttcgatgccg
     3661 ttttcacgca aaacgcgcac ggcaggtgtt atcggataag tgtgtttact catttttcgt
     3721 catgcaaaac atggaaatcg gtcaagcgca atgttaccat atatcccctt tatcgaagga
     3781 aaccgatatg gatatccgat atttcggcac aacgccccgc tattccgaag ccgtcggcgc
     3841 aaacggcttg attttcctct ccggcatggt tcccgaaaac ggtgaaacgg ctgccgaaca
     3901 gactgccgac gtgcttgccc aaatcgaccg ctggctggcg gaatgcggct cggacaaagc
     3961 gcacgttttg gatgcggtca tctatctgcg cgatatgggc gactatgcgg aaatgaacgg
     4021 cgtgtgggac gcttgggttg ccgccggcag aacgcccgcc cgcgcctgcg tggaagcacg
     4081 gctcgcccgt ccggagtggc gcgtcgaaat caaaataacc gccgtgaaga gagacgcggc
     4141 aactgcataa acagatttcc tttatatttc agagtacgca aatgatgaaa accttactgc
     4201 cctttttcgg cgtggtgcgc gtcagcggcg aagaccgcca aaccttcctg cacggacagc
     4261 tttccaacga catcaaccac cttcagaccg ggcaggcgtg ttatgcgact tacaacacgc
     4321 ccaaaggccg cgtcatcgcc aatatgattg tcgtcaaccg aggcggcgat ttgctgctga
     4381 ttatggcgca agacctgctt gaagcaacgg tcaaacgttt gcggatgttt gtgttacgcg
     4441 ccaaagccgt ttttgaaatc cttgaagatt acgccgtcgg cgcggaatta gaagcatccg
     4501 ccgaaccgct tgccgcgcaa gaacccagcc tcgcctttac cgccgaatgc ggttcagacg
     4561 gcatctgttc cgtcgtcctg ccacatcggg gcattttgca catcgccccc aaaaacgcct
     4621 tgccgcctta tgatgccgcc gccgaaaacg cgtggcggct gcacgaaatc cgtagcggtt
     4681 atccgtggat atgcgcggct accaaagaaa ccgccgtcgc ccaaatgctc aaccagcaca
     4741 tcatcggcgg cgtacacttc aaaaaaggct gctatccggg acaggaaatc attgcccgtg
     4801 cccagtatcg ggggcaggtc aaacgcggct tggcggtatt gtcgggaaat tcggccgtcg
     4861 aagcaggaac cctgctgacg gcggacggcg aagaggccgg catcgtactc gacagcgttc
     4921 aagattcgga aaactttacc gcccttaccg tgattaaatt ctccgccgca caaaaaacac
     4981 tgaccgtccc aaacggcggt atcttcaaag cggttcatct cttctttaaa accgaaaatg
     5041 cagaataagc cgccgatgcc gtctgaagcc cttcagacgg cattgccgcc ccgccaatcg
     5101 gttacaatgc cgcctttcta attttcaaac ccaaaccgcc atgtccaaaa aaaccaaaca
     5161 agaactcgaa aacaacaaac tcagcaaacg cctgcgccac gccgtcggcg acgcgattaa
     5221 cgatttcaat atgatcgagc cggacgacaa aatcatggtc tgcctctccg gcggcaaaga
     5281 cagctatgcc ctgttagaca tcctgcgcca gctccaagcc agcgcgccga ttgatttcca
     5341 actggttgcc gtcaatctcg accaaaagca gccgggcttc cccgaagaag tattgccgac
     5401 ctatctcgaa agcatcggcg ttccctacaa aatcgttgaa gaagacacct actccaccgt
     5461 caaacgcgta ttagacgaag gcaaaacgac ttgttcgctg tgcagccgcc tgcgccgcgg
     5521 catcctctac cgcactgcaa aagaattggg ctgcaccaaa atcgccttgg gacaccaccg
     5581 cgacgacatc ctcgccacct tgtttttaaa tatgttttac ggcggcaaac tcaaagccat
     5641 gccgcccaag ctggtgagcg acaacggcga acacatcgtc atccgcccgc tggcgtatgt
     5701 gaaagaaaaa gacttaatca aatacgccga attaaaacaa ttccccatca tcccgtgcaa
     5761 cctctgcggc tcgcaaccca acctgcaacg ccaagtcatc ggcgatatgc tgcgcgattg
     5821 ggacaaacgc ttccccggcc gcatcgaatc gatgttctcc gccctgcaaa acgtcgtccc
     5881 ttcccacctt gccgataccg aactcttcga ctttgtcgga ttggaacgcg gtcaaagcct
     5941 gaaacacggc ggcgatttgg cgtttgacag cgaaaaaatg cccgaacgtt tttcagacgg
     6001 cagcgaagaa gacgagagcg aaatcaaaat cgagccgcaa aaagccgaac gcaaagtcat
     6061 caatattctg gcgaacaagc cgaaaacctg cggctcatag acctccgccg tcaaaaatgc
     6121 cgtctgaagc ccgtttgg
//
LOCUS       DAWGRZ010000127         6101 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000127 DAWGRZ010000000
VERSION     DAWGRZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.127"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(1048..3795)
                     /gene="tbpA"
                     /locus_tag="WHT44_001599"
     CDS             complement(1048..3795)
                     /gene="tbpA"
                     /locus_tag="WHT44_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ2533762.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEAAGRGVQSFNRLVPVEDSSNYAYFIVKEECKNGSYETCKANP
                     KKDVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILERT
                     QQTFDTRDMTVPAFLTKAVFDENKKQAGSLSGNGKYAGDHKYGGLFTNGENGALVGAE
                     YGTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADG
                     SDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGS
                     DLRHQDYYYQNANRAYSSKIPTQNGNQKISPNGSQDKPYWVSIGGGNVVTGQICRSGN
                     NTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTL
                     SWNAGIVLKPVDWLDLTYRTSTGFRLPSFSEMYGWRSGDKIKAVKIDPEKSFNKEVGI
                     VFKGDFGNLEASWFNNAYRDLIVRGYEAQIKNGKEEAKGDPAYLNAQSARITGINVLG
                     KIDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYD
                     QPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTI
                     KKHFTLRAGVYNLLNHRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMK
                     F"
     gene            complement(3882..6026)
                     /locus_tag="WHT44_001600"
     CDS             complement(3882..6026)
                     /locus_tag="WHT44_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ2533763.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGGSFDLDSVDTEAPRPAPK
                     YQDVSSEKPQAQKDQGGYGFAMRFKRRNWHPMAMPKENEVKLKNDDWEATGLPTEPKK
                     LPLKQESVISKVQANNGDNNIYTSPYLAQSNHQNGNTGNGVNQPKNWAKGYENFQYVY
                     SGWFYKHAKREFNLQGEHKSAKNGDDGYIFYHGKEPSRQLPASGNIIYKGVWHFVTDT
                     KQGQKFNDILETSKGQGDKYSGFSGDEGETTSNRTDSDLNNNHEGYGFTSNLEVDFNN
                     KKLTGKLIRNNRVTNATTGDKHTTQYYSLEAQVTGNRFNGKAMATDKPGTGETKLHPF
                     VSDSSSLSGGFFGPKGEELGFRFLSDDQKVAVVGSAKTKDKTENGAAASGGAGAAASG
                     GAAAMPSENGKLTTVLDAVELTHGGTAIKNLDNFSNAAQLVVDGIMIPLLPEASESGN
                     NQANQGTNGGTAFTYKTTYTPKSDEKDTQAGTAANGDQAASNTAGDTNGKTKTYEVEV
                     CCSNLNYLKYGLLTRKTAGNTGEGGNGSPTAAQTAQGAQSMFLQGERTDENKIPSEQN
                     VVYRGSWYGHIANGTSWSGNASNATSGNRADFTVNFGEKKITGTLTAENRQAATFTIE
                     GMIQGNGFEGTAKTGDGGFALDPKNTEASHKASINATVTGGFYGPKAEEMGGWFAYPG
                     DSQAQPSASGSGTSAANSATVVFGAKRQQLVQ"
BASE COUNT         1288 a         1549 c         1549 g         1715 t
ORIGIN      
        1 ctataccgtc gttcccacgg tcaggatttc agattgcgga catctgtcag aaaagacaaa
       61 aaaccttccg ccgtcattcc ctacaggcga gaatccgatc cgttgaaatt cggttgtttt
      121 aaataaattc ttgcagcttt gattttctgt ttttccgata acgccgtaac tttgaaacgc
      181 gaaagcggta atccgatccg ttgggatttt gcaacttcaa atcaatccgc aaactgaaat
      241 cccgtcattc ccgcgcagtc gtgaatccga acgcgtccgc acgaaaacct gcatcccgtc
      301 attcccacgg aagtgggaat ctaggacgta aaatctcaag aaaccgtttt atccgataag
      361 tttccgcacc gacagacctg gattcccgcc tgcgcgggaa tgacgaaatt tcggcgagcc
      421 gtagggtggg ctgtagggtg ggcttcagcc caccaatttc accgcatcaa aggtttttgg
      481 gaatacggcg gtttcggttt tcggtgggaa tgggcggaat ggataacatt gacggagttg
      541 ggggaatagg tggaaacggt gggattggtg ggctgaagcc caccctacag ccagccctat
      601 gtccacgctg cacatcctac gcccgccttg cgtttcttat aagcagcatt ttacgcaata
      661 ctttgcgtcc gctgaaaaaa tttcaaacgg tcttttttat caaatgcgaa aaatataacg
      721 tcattcccgc gcaggcggga atctaggtct gtcggtgcgg aaacttatcg gataaaacgg
      781 tttctttaga ttttacgccc tggattactg cctgcgcggg aatgacggga tttgaggttt
      841 ctgtttttga ttttccgctt tcgagaaaat gacggtttag agttacccga aacctcaaaa
      901 aaaccgaaac cgaacggtct ggattcccgc ctgcggggaa tgacgggttt caagattacg
      961 gtgtatcggg aataatggaa aacggtggga attgtgtaaa aaatgccgtc tgaagccttt
     1021 cagacggcat ttgcggcgtt tggacgttta aaacttcatt tccaagctaa atgtgtagtt
     1081 gcgaccgggg gcggcatatc ggttgtaaac gccgacattt ttgtgttggt tgactgcgcc
     1141 gccggcagtt tgccgcacat tttcccaagt aacatagcgg tggttgagga ggttgtacac
     1201 gcccgcacgg agggtgaagt gttttttaat cgtgtaataa ccggacacat ccacaatata
     1261 ccaagggcgg gtacggcgcg cggtggcttt tgtattgcgg ctgttgccgt tgagcaaagc
     1321 ccggctgccc aacaactctg tgatttcctt ggctttggaa taagtcagca taccgttcac
     1381 accccattta ccttccggtt ggtcatagcc caagccgacg acatagcgcg agggttggat
     1441 ggcatcaaac aggtgtgatt gaatatcggt gcggtctgcg cgttttttga tgtcgcggac
     1501 acggacacga ttataggcaa atgtagaata ccaaccttcg ggcaatttat cccatacgcc
     1561 gttccaatcg attttgccca aaacattgat gccggtaatc cgcgcgcttt gggcattgag
     1621 gtaagctggg tcgcctttgg cttcttcttt gccgttttta atttgcgctt cataaccccg
     1681 gacaatcaaa tcgcggtagg cattgttgaa ccaacttgcc tccaagttgc cgaaatcgcc
     1741 tttaaacacg atgccgactt ctttgttgaa cgatttttcc ggatcgattt tgacggcttt
     1801 tattttatcg cccgaccgcc agccgtacat ttccgaaaac gagggcaggc ggaagccggt
     1861 tgaagtgcgg taagtcaaat ccagccagtc gacaggtttg aggacgatgc cggcgttcca
     1921 ggacagggtg cggtgcgtgc cggtggaaac gctgccgtcg tccgaatgcg tgctgcggta
     1981 gtcgtagcgc aagcccgcgc cgacatccgc ccacctgccc aaacggacat tgtcccgaac
     2041 tgccgcgtaa tagcttttgc cgttgatgct gcgcggcgtg cagtccgtat aagtattgtt
     2101 gcccgagcgg cagatttgcc ccgtaaccac atttcccccg cctatgctga cccaataggg
     2161 tttgtcttgg ctgccgttgg ggctgatttt ttgattgccg ttttgagtgg gtattttcga
     2221 cgaataggcg cggttggcat tttgataata ataatcctga tggcggaggt cggaaccaaa
     2281 acggtcaaaa ccgaggttga cgctcaggtt gtggcggatt ttggcggtat cgaaggattt
     2341 tttgaatgcc gcctgcaaga gcctgtggct ttccccgtaa atcacgcggt cggatttgta
     2401 ataggaaaac ggcttgtcgg cactcggacg gcaatatttg tccgaaccgt cggcagaaca
     2461 gtgcgtctgc tgaaaatgat tatccaaacc gataccctgc cggtcgtaag agaggcgggc
     2521 ataatccgcc caagtgtctt tatcggcatt ggtatagaca tattccaaac cgtagcggct
     2581 tttggtgtgc gtctcgtcgt aaaacacgcc cgtaccgtat tccgcgccca ccagcgcacc
     2641 gttttcgccg ttggtaaaca gaccgccgta tttgtggtcg cccgcgtatt tgccgttgcc
     2701 ggacaaagaa cccgcctgtt ttttattttc atcaaaaacc gccttggtca ggaatgccgg
     2761 aaccgtcata tcgcgcgtgt cgaaagtttg ttgcgtgcgt tcgagtatgc cgccgatgta
     2821 gtgccgtttg ttttcaaaac gaaaacccgg gcggaacagc cacgaccggc tttcgtatga
     2881 aagcggatcg gcgaggaagc ggttgggacc cgtgtagtct cgggtggaaa ccgtttgacg
     2941 ttcgtctttg ccgacaacat cttttttcgg attcgcttta cacgtttcat aactcccgtt
     3001 tttgcattct tctttaacga tgaaataggc gtaattgctg ctgtcttcaa ccggcaccag
     3061 cctgttaaag ctctgaacgc cgcgtccggc ggcttcgtgg gcgcggattt cccccgcgcg
     3121 ccgacccgtg tggatcagca aagcctccgc accgccgatg cgccccgcca gcgcgatgga
     3181 ttgggtaagc ccccggtttt tgccggaata ggcggtttta ctctgaatcc cccactgcct
     3241 gccttccccg ataacatcgt cggcggtttt ggtttgaaat gcgaccgagc ctgccaatgc
     3301 gccgctgcct tgttcgaccg agtttgagcc tttgctgatt tcgacagctt tgacgttttc
     3361 atactcgatt tcattgattg cgccgctgct gcccgccgtc ctcgtcccgc ccaatgccgc
     3421 ctgcgcggtg taggactgta tttgcgccaa gccgtctacc gtcaaggaaa cgcggttttt
     3481 gtccataccg cgtatcgaat agcccgagct tgcgccgcgc ccctgttcga cgacggcgat
     3541 gccggggtcg tagcgagtca ggtcgcggat atcgagtacc tgttccttgc tgagggtgtc
     3601 ggcggttttg accaatttgc ccaaaccggt tacttcgtta tcgcggcggg ttttctgttt
     3661 tttggctttt acctgtatgg tatccaactg tttttcctgt gcttgtccgg cttgcacatt
     3721 ttctgcataa acgggcagcg cagtcatcag cgacaggcat aaaatattta atcggaacaa
     3781 atgttgctgt tgcatagtgt ttccctactc ttcgctttca gacggcatcg gaaggagcga
     3841 tgccgtctga agccttattc tcgattgttc ggcagccgta cttattgcac aagctgttgg
     3901 cgtttcgcac cgaataccac ggtcgcgctg ttggcggctg acgtacctga ccccgaagcg
     3961 gacggctgcg cctgactgtc gcccggatag gcaaaccatc cgcccatttc ttcggcttta
     4021 ggcccgtaaa aaccgccggt tacagtggca ttgatgctcg ctttatggga ggcttccgta
     4081 tttttcggat ccagcgcaaa gccgccatca ccggttttcg ccgtaccttc aaagccgttg
     4141 ccctgaatca ttccctcaat ggtaaaggtt gccgcctgcc tgttttcagc ggttaacgtg
     4201 ccggtaattt ttttctcgcc aaaattcacg gtaaagtccg ccctgttgcc actcgttgca
     4261 ttggaagcat tgccgctcca gcttgtgccg ttggcaatat gcccgtacca agacccccga
     4321 taaacgacgt tttgctcgct tggaatcttg ttttcatcgg tgcgctcgcc ttggaggaac
     4381 atactttgtg caccctgtgc cgtttgggcg gcggttgggc tgccgttgcc gccttccccc
     4441 gtattgccgg cagtcttgcg cgtcagcaac ccgtatttca gataattgag gttggaacag
     4501 cagacttcga cttcataggt ttttgttttg ccattggtat cacctgccgt atttgaagcg
     4561 gcttgatcgc cattcgccgc cgtacctgct tgggtgtctt tttcatcgct tttcggcgtg
     4621 taggttgttt tgtaggtaaa ggctgttccg ccatttgtac cttgattggc ttgattgttc
     4681 ccactttcgg aagcctcggg cagaagcgga atcataatgc cgtcgacaac cagttgggcg
     4741 gcattgctga agttgtcgag atttttgatt gctgtgccgc cgtgcgtcag ctcgaccgca
     4801 tccaaaaccg tggtcagctt accgttttca gacggcatag ctgccgcacc gcccgatgct
     4861 gccgcacctg cgccgcctga agccgctgcg ccattttccg ttttgtcttt ggttttcgcg
     4921 ctgccgacaa ccgcaacttt ttgatcgtcg ctcaaaaagc ggaaacccaa ttcctcaccc
     4981 ttcggaccga aaaagccgcc gctcaaagaa gacgagtcgg aaacaaaggg atgtagtttg
     5041 gtttctccag tgccgggctt gtcggttgcc atcgccttgc cgttgaagcg gttgcctgtt
     5101 acttgagcct caaggctgta atattgcgtg gtatgtttgt cgccagtagt agcgtttgta
     5161 actctattat tgcgtatcaa tttacccgtc aattttttat tattgaaatc cacttctaaa
     5221 tttgaggtaa aaccataacc ctcgtgatta ttattaaggt cggaatcagt tctattggaa
     5281 gttgtttcgc cttcatcgcc cgaaaatccg ctgtacttat ctccttgccc ttttgaagtt
     5341 tcaagaatat cgttaaattt ttgtccctgt ttcgtatcgg ttacaaaatg ccacacacct
     5401 ttgtagataa tgtttccaga agcgggaagt tgtcgggaag gttctttgcc gtgataaaag
     5461 atataaccgt cgtcgccgtt ttttgcactt ttatgttccc cctgtaagtt aaactctcgt
     5521 ttggcatgtt tataaaacca gccggaataa acatattgga aattttcgta accttttgcc
     5581 caatttttag gttggtttac accgttgcca gtgttgccgt tttgatggtt tgattgcgcg
     5641 agataggggg aagtgtaaat attgttgtcg ccattgtttg cttgtacttt tgaaatgacg
     5701 gattcttgtt ttaatggcag tttcttgggt tctgtcggta atcctgtcgc ctcccaatca
     5761 tcattcttta atttaacctc attttcttta ggcattgcca taggatgcca attcctccgc
     5821 ttgaagcgca ttgcaaaacc gtatccgcct tggtcttttt gggcttgcgg tttttcggaa
     5881 gaaacatctt gatactttgg cgcgggacgc ggggcttcgg tatcgacaga atcaagatcg
     5941 aaactgccgc cgcctccgcc caaacaagca ctcaacaaaa acaccggcag caccatagca
     6001 gcctgattta ccaatggatt gttcatataa acccaattca attaaagaat gatagagatt
     6061 attattttat ttatttttaa caaatttgca aatacttttt t
//
LOCUS       DAWGRZ010000128         6090 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000128 DAWGRZ010000000
VERSION     DAWGRZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.128"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            297..995
                     /locus_tag="WHT44_001601"
     CDS             297..995
                     /locus_tag="WHT44_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ2533764.1"
                     /translation="MKSKHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(1052..2056)
                     /locus_tag="WHT44_001602"
     CDS             complement(1052..2056)
                     /locus_tag="WHT44_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ2533765.1"
                     /translation="MQRQTELKNWLQTIYPERDFDLSFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            2126..4534
                     /locus_tag="WHT44_001603"
     CDS             2126..4534
                     /locus_tag="WHT44_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ2533766.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPSVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYITGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            4639..5763
                     /locus_tag="WHT44_001604"
     CDS             4639..5763
                     /locus_tag="WHT44_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ2533767.1"
                     /translation="MKIKALMIAAALLAAADVHAAPQKAKTASAKAAKAAKAAKAAKA
                     AKAAKAAKAAKVAKVAKVAKVAKVAKVAKVAKVAATAQKEAAPAQQQGGIRFSDGIAA
                     VADNEVITRRRLAEAVAEAKANLPKEAQISESELSRQVLMQLVNQSLIVQAGKRRNIQ
                     ASEAEIDAVVAKNSALKNLSPAQRRDFADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQ
                     AQKQGITLPEGAPLRQYRAQHILIKADSENAAVGAESTIRKIYGEARSGTDFSSLARQ
                     YSQDASAGNGGDLGWFADGVMVPAFEEAVHALKPGQVGAPVRTQFGWHIIKLNEVRDA
                     GTPQERIRNSVRQYIFQQKAEQATVNLLRDLHSGAYVDIR"
BASE COUNT         1516 a         1844 c         1592 g         1138 t
ORIGIN      
        1 ttgaaagggg tgtaaccctt ccgaaccagg gcaacgcata gggcggtgct ttatgtgccg
       61 tcctgtgtgt tggaacatca ttagttcccc aacagccgta tccgtctggg gcgacggcgc
      121 gtattgtacg acagaaaccg cttcagacgg catcgggttt gatgccgtcc gaagcggttt
      181 ccccaaacga aaacgtcctt ttccggaggc aaactgtatc aacacgcgga aatgcaataa
      241 aatagccgga ttccgattga tttccaacat ctgtttccaa catcacggag agccgtatga
      301 aatccaaaca cctcgccctc ggcgttgccg ccctgttcgc acttgccgcg tgcgacagca
      361 aagtccaaac cagcgtcccc gccgacagcg cgcctgccgc ttcggcagcc gccgccccgg
      421 cagggctggt cgaagggcaa aactatactg tccttgccaa cccgattccc caacagcagg
      481 caggcaaagt cgaagtcctt gagtttttcg gctatttctg tccgcactgc gcccacctcg
      541 aacctgtttt aagcaaacac gccaagtctt ttaaagacga tatgtacctg cgtaccgaac
      601 acgtcgtctg gcagaaagaa atgctgacgc tcgcacgcct cgccgccgcc gtcgatatgg
      661 ctgccgccga cagcaaagat gtggcgaaca gccatatttt cgatgcgatg gtcaaccaaa
      721 aaatcaagct gcaagagccg gaagtcctca aaaaatggct gggcgaacaa accgcctttg
      781 acggcaaaaa agtccttgcc gcttacgaat ctcccgaaag ccaggcgcgc gccgacaaaa
      841 tgcaggagct gaccgaaacc tttcaaatcg acggtacgcc cacggttatc gtcggcggca
      901 aatataaagt cgaatttgcc gactgggagt ccggtatgaa caccatcgac cttttggcgg
      961 acaaagtacg cgaagaacaa aaagccgcgc agtaagcccg tttgaaaaat gccgtctgaa
     1021 acttggtttt cagacggcat tttgattggg tttaaaacgt aaagcccgtt tccagttctt
     1081 catcgccgac cagttcgacc aagagcgcgt agagcggggc gagttcggca taacggcgcg
     1141 atacgcggcg caggtaattg aggaaacgcg ggatttcggg acggtatttg tctttgccgt
     1201 cacggtagta caggcgtgcg aagatgcctg caaccttcaa gtgccgctgc acgcccatcc
     1261 attcgaacca gcggtaaaac tcgtcaaacg cttcggggac gggcaagccg gcagcccgcg
     1321 ccttttccca gtagcggata accaagtcca agacaaattc ttcttcccat tcgataaagg
     1381 catcgcgcaa cagcgacacc aaatcgtagg aaatcgggcc gtaaagcgcg tcttggaagt
     1441 ctaaaacgcc cggtctgccg cgcgtcagca tcaggttgcg gacgataaag tcgcggtgca
     1501 catagacttt gggctgcgcc aacaggggcg gcagcagcgt atcgacggtt tgctgccaaa
     1561 gttggcgttg tttgaatgtt aattcgcgcc ccaattcttt tgcgacaaac cattccggga
     1621 acaggttgat ttcgcgcagc atcgtttcac ggtcatattc gggcaaaacc ccttcacggc
     1681 ttgccttctg caattcgacc agctcgccga ttgcctccaa aagcagggct ttgtgcgccg
     1741 cttcgccctg ctcctgaagc atcgcggtca aaaacgtcgt attgcccaag tcgttcaata
     1801 ccacaaaccc caaatccgtg tccgcgtgca atacctgcgg tacattgacc atgtcaaaca
     1861 gtttctgcac tttcaaataa ggtgcgacac tcatcttgtc gggcggtgca tccatgcaga
     1921 cgacactgct gccgtctgaa aacgttgcac ggaaatagcg gcggaaatca gcatccgccg
     1981 ccgcgaagga cagatcgaag tcccgttcgg gatagatggt ctgaagccaa tttttcagtt
     2041 cggtttgccg ttgcataaca gtactaaagc atttcaggtt acaataaacg ctattctaac
     2101 tggcaaaccg acttgagggg cgattttggc tcgtttattt tcactcaaac cactggtgct
     2161 ggcattgggc ttctgtttcg gcacgcattg cgccgccgcc gatgccgttg cggcggagga
     2221 aacggacaat ccgaccgccg gaggaagcgt tcggagcgtg tccgaaccca tacagcctac
     2281 cagcctgagc ctcggttcga cctgcctgtt ttgcagtaac gaaagcggca gccccgagag
     2341 aaccgaagcc gccgtccgag gcagcggcga agcatccatc cccgaagact atacgcgcat
     2401 tgttgccgac aaggtggaag gacagtcgca ggtgcaggtg cgtgccgaag gcaacgtcgt
     2461 cgtcgaacgc aaccggacga ccctcaatac cgattgggcg gattacgacc agtcgggcga
     2521 caccgttacc gcaggcgacc ggttcgccct ccaacaggac ggtacgctga ttcggggcga
     2581 aaccctgacc tacaatctcg agcagcagac cggggaagcg cacaacgtcc gtatggaaac
     2641 cgaacacggc ggacggcggc tgcaaagcgt cagccgcacc gccgaaatgt tgggcgaagg
     2701 gcattacaaa ctgacggaaa cccaattcaa cacctgttcc gccggcgatg ccggctggta
     2761 tgtcaaggcc gcttccgtcg aagccgatcg ggaaaaaggc ataggcgttg ccaaacacgc
     2821 cgccttcgtg ttcggcggcg ttcccatttt ctacacccct tgggcggact tcccgcttga
     2881 cggcaaccgc aaaagcggcc tgcttgttcc ctcactgtcc gccggttcgg acggcgtttc
     2941 cctttccgtt ccctattatt tcaaccttgc ccccaatctc gatgccacgt tcgcgcccag
     3001 cgtgatcggc gaacgcggcg cggtctttga cgggcaggta cgctacctgc ggccggatta
     3061 tgccggccag tccgacctga cctggctgcc gcacgacaag aaaagcggca ggaataaccg
     3121 ctatcaggcg aaatggcagc atcggcacga catttccgac acgcttcagg cgggtgtcga
     3181 tttcaaccaa gtctccgaca gcggctacta ccgcgacttt tacggcaaca aagaaatcgc
     3241 cggcaacgtc aacctcaacc gccgtgtatg gctggattat ggcggcaggg cggcgggcgg
     3301 cagcctgaat gccggccttt cggttctgaa ataccagacg ctggcaaacc aaagcggcta
     3361 caaagacaaa ccgtatgccc tgatgccgcg cctttccgcc gattggcgca aaaacaccgg
     3421 cagggcgcaa atcggcgtgt ccgcacaatt tacccgcttc agccacgaca gccgccaaga
     3481 cggcagccgc ctcgtcgtct atcccgacat caaatgggat ttcagcaaca gctggggtta
     3541 cgtccgtccc aaactcggac tgcacgccac ctattacagc ctcaaccgct tcggcagcca
     3601 agaagcccga cgcgtcagcc gcactctgcc cattgtcaac atcgacagcg gcgcaacttt
     3661 tgagcggaat acgcggatgt tcggcggcgg agtcctgcaa accctcgagc cgcgcctgtt
     3721 ctacaactat attcctgcca aatcccaaaa cgacctgccc aatttcgatt cgtcggaaag
     3781 cagcttcggc tacgggcagc tctttcgcga aaacctctat tacggcaacg acaggattaa
     3841 caccgcaaac agcctttccg ccgccgtgca aagccgtatt ttggacggcg cgacggggga
     3901 agagcgtttc cgcgccggca tcggtcagaa attctatttc aaggatgatg cggtgatgct
     3961 tgacggcagc gtcggcaaaa aaccgcgcaa ccgttccgac tgggtggcat ttgcctccgg
     4021 cagcatcggc agccgcttca tcctcgacag cagcatccac tacaaccaaa acgacaaacg
     4081 cgccgagaac tacgccgtcg gtgcaagcta ccgtcccgca cagggcaaag tgctgaacgc
     4141 ccgctacaaa tacgggcgca acgaaaaaat ctacctgaag tccgacggtt cctattttta
     4201 cgacaaactc agccagctcg acctgtccgc acaatggccg ctgacgcgca acctgtcggc
     4261 cgtcgtccgt tacaactacg gttttgaagc caaaaaaccg atagaggtgc tggcgggtgc
     4321 ggaatacaaa agcagttgcg gctgctgggg cgcgggcgtg tacgcccaac gctacattac
     4381 cggcgaaaac acctacaaaa acgctgtctt tttctcactt cagttgaaag acctcagcag
     4441 tgtcggcaga aaccccgcag acaggatgga tgtcgccgtt cccggctata tccccgctca
     4501 ctctctttcc gccggacgca acaaacgacc ctgaccgtcg gaaacccggc aggagcaccg
     4561 ttcccgaaca agacggcatt ccaccgacaa ccccaaaccc gccatcaaag gtaggattca
     4621 aacgataagg aaagaatgat gaaaatcaaa gccctgatga ttgccgccgc attgctggca
     4681 gcagccgatg tccacgccgc accgcaaaag gcaaaaaccg catccgccaa agctgccaaa
     4741 gctgccaaag ctgccaaagc tgccaaagct gccaaagctg ccaaagctgc caaagctgcc
     4801 aaagttgcca aagttgccaa agttgccaaa gttgccaaag ttgccaaagt tgccaaagtt
     4861 gccaaagttg ccgccacggc gcaaaaagaa gccgcacccg cacaacagca gggcggtatc
     4921 cgcttttcag acggcattgc cgccgttgcc gacaacgaag tcatcacgcg ccgccggctt
     4981 gccgaagccg ttgccgaagc caaagccaac ctgcccaaag aggcgcagat aagcgaatcc
     5041 gagctgtccc gacaggtgct gatgcagctt gtcaaccaat ccctgattgt acaggcgggc
     5101 aaacgccgca acattcaagc aagcgaagcg gaaatcgatg ccgtcgtcgc aaaaaattcc
     5161 gccctcaaaa acctcagccc cgcccaacgc cgcgattttg ccgacaacat cattgccgaa
     5221 aaagtccgcc agcaggcagt gatgcagaac agccgcgtga gcgaagctga aatcgatgcc
     5281 ttcctcgagc aggcgcaaaa acaaggcatc accctgcccg aaggcgcacc gttgcgccaa
     5341 taccgcgccc aacacatcct gattaaagcc gacagcgaaa acgccgccgt cggcgcggaa
     5401 agcaccatcc gcaaaatcta cggagaggcc cgcagcggca cagacttttc cagcctggcg
     5461 cgccaatatt cgcaagacgc gagcgcgggc aacggcggag atttgggctg gtttgccgac
     5521 ggcgtgatgg ttcccgcctt tgaagaagcc gtccacgcgc tcaaacccgg acaggtcggc
     5581 gcgcccgtcc gcacccaatt cggctggcat atcatcaaat tgaacgaagt gcgcgatgcc
     5641 ggcacacctc aggaacgtat ccgcaattcc gtgcggcaat acatcttcca acaaaaagcc
     5701 gaacaggcaa ccgtcaacct gttgcgtgac ctgcattccg gcgcgtatgt cgacatccgc
     5761 taaggcggtt tgaagcaaaa agccataccg atcggcaaaa atccgggcgg tatggctttt
     5821 tggatttcga gttactttta caccgtcatt catcattccc gcgaaagcgg gaatctagaa
     5881 acgaaaagta acaggaattt atcgggaatg gctggagttt aaaggactgg attcccgccg
     5941 tcgcgggaat gacgggattt tgggttgtgg taatttatcg gaaaaacaaa aaaacctatg
     6001 ccgtcattcc cgagcaggcg ggaatccggt tatttaaaac tgcagaaatt tatccgaagc
     6061 aacaacaatc tttccatcgt cattcccgcg
//
LOCUS       DAWGRZ010000129         5985 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000129 DAWGRZ010000000
VERSION     DAWGRZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.129"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(20..1423)
                     /gene="mtrE"
                     /locus_tag="WHT44_001605"
     CDS             complement(20..1423)
                     /gene="mtrE"
                     /locus_tag="WHT44_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ2533768.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPEDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQVQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYAAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(1478..4681)
                     /gene="mtrD"
                     /locus_tag="WHT44_001606"
     CDS             complement(1478..4681)
                     /gene="mtrD"
                     /locus_tag="WHT44_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ2533769.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LRATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADVGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSLEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(4693..5931)
                     /gene="mtrC"
                     /locus_tag="WHT44_001607"
     CDS             complement(4693..5931)
                     /gene="mtrC"
                     /locus_tag="WHT44_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ2533770.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGISLNRSRITAPISGFIGQSKVSEGTLLNAGDATVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPAVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWASSENQA
                     AAPQSGVQTASEAKPASEAK"
BASE COUNT         1335 a         1707 c         1618 g         1325 t
ORIGIN      
        1 cattttattg cccgaccggt tatttgtcgg tttgggtatc ccgtttcaat ccgccgccga
       61 gtgccttgta caaatcggca aggttttcgg cgcgggtcag ttgtgccgac aaagccgcac
      121 cctccgccgc atagctgctg cgttccgcat cgagcaagtc gagcgcgccg gatacgccgt
      181 gcttgtaacg caggccgacc aagcgcaacg cttctttaga ggcgcggctt tgtttgctta
      241 aagcgtcata ggctttatcc agctgctcgc gcgccgccaa tgcgtttgcc acgtcttgaa
      301 atgcggattg gacggcggat tcataggcaa cgatttgtac ctgttggcgc agcttggcta
      361 catcaaggtt cgccttgttc gtaccccagg taaaaatcgg cagggtaata gacggcgcga
      421 acgaccaaac gcccgtgccg cttttgaaca acccacccaa ttcggcagaa cccgtaccga
      481 cggttccggt caggcggatg gatgggaaaa aggcggcgcg tgccgcaccg atattggcgt
      541 ttgcctgttt gagcgcgtgt tcggcagcac ggatatcggg acggtcgagc aatacttcgg
      601 aactcaaacc ggccggcagt ttttcaacaa aaaactgctt gtccagcggc aaaccggcag
      661 gcaggtcttc gggtatcggt tggttaatca aggttgccaa ggcattgcgc gcctgttcgc
      721 ggctgcgcgc ggcatgggca taatcggctt tggcagattc gatcagggct tcctgctgac
      781 gtagggcgac ggcggaaatc acgcctgcct tgtaacgtaa ttcggacagc ttgtaggttt
      841 cctcgcgcgt tttcaaaaca cgttgcgcca aagacatcgc ttcttcggcg taacgttcgt
      901 tgaaataggc tttggcaacg gtggcaatca ggctcaaatg tgccgcatcg cggttggcgg
      961 tgctggcgaa atagccttgc agtgccgcct cgctgctgct gcgtacacgc ccgaacagat
     1021 cgagttcgta agatgccgca cccagtccga ctttgtagct gctgcttaca ttgccgccgc
     1081 tcaagctgcc ttggcgcgag tcgttcgcat tggcggcaag cgtgggcagg aggttgttgc
     1141 gctcaatcat gtattgtttg cggtagattt cgctgttcaa tacggcggta cgcaaactgg
     1201 tattgcgctc gagtgcgatg tcgatcagct tttgcaggcg cgggtcggca aaatagtcat
     1261 gccaacctaa atcgacggcg cggatgccgc tgtcggcggt atcgtttttg aacgtttcgg
     1321 caacttcgac tttgggctgc tcgtattggg gaatcatggt gcaggcagac aatgcaaagg
     1381 ctgctgcaac agaagtcaag gtggttttca atgtagtatc cataaaaaag tcctgatgcc
     1441 gtctgaaaac ccgtgggcgt tcagacggca tggttgctta atgttggctg tcgtccgaac
     1501 cggtgatgcc cgcttcggcg gcgtgtttta ctgccatttc gtgttcgtgc gcggtttctt
     1561 tgaagaattt gcgcaccacc acatagaaaa gcggaacaag gaacacggac aagagcgtgc
     1621 cgatgagcat cccccagaat acggttgtac cgatggcgcg ctggctggca gaacttgcac
     1681 cgccggcaat atacagggga accacgccca aaataaaggc gaacgaggtc atgataatcg
     1741 gacggaaacg caggcgggcg gcttccaaag cggcttcaac cgcgcttttc ccttgcgctt
     1801 gaaggtcttt ggcaaattcg ataatcaaaa tcgcattttt cgcactcaaa cccatcacgg
     1861 taacgaaacc gacttgaaag tagatgtcgt tggcgaacga gggaacgctg cccaacagtc
     1921 cttcaaacag gttgcgcccg gttacgcccg cagccgcacc gatcaaaccc aacggaatca
     1981 caaggatgac cgccagcgga atcgaccagc tttcataaag cgcggcaagt accaaaaata
     2041 cggctgcaac cgccaaaccg tacaaaatca gggtttgcga gccgcctttt gcctcttcgc
     2101 gcgactgtcc gccccactcc aggctgtaac cgccgcccaa ttcgtcaacc attttttgaa
     2161 ccgccgccat agcctgcccg gtggaaacgc cggttgcagg cgaagcggac agcttcatcg
     2221 aaggataacc gttgaagcgt acgctctgtt ccgtaccgtt ttcccaagaa acagtagcaa
     2281 tggtggaaag cggtacggcg acgccggatt tgttcggcac ggtcaggttc aaaatatcgg
     2341 caggctgcat acgggcatcc tcgtcggcct gcaccatcac gcgttgcaga cggccttggt
     2401 tcgggaagtc gctgacataa gacgaactca gcgcgcttgc caatgcggtg cggatgtcgg
     2461 caaacgaaat gccttgcgcc gccgccgcgg cacggttgat gtcgattttc aactgcggcg
     2521 agtcttccaa accgccagca cggacggtgc tggggtcaaa caaaccgctg gcacgcattt
     2581 tctgaatcaa ctcgttgcgc ttcgccagca atgcggtatg gccggtattg ttgcggtctt
     2641 gcaggttgat gctcagaccc gaaccgttgc ccaactccag aatcggaggc gggacgacgg
     2701 cgatgccaaa accgtcttta agcgtcccca tcatcatacc cgtcagcttg ccggcaatcg
     2761 caacggcatc gctgccgggc gcggtacgct cgttccaatc tttcaatatg gcaaaaccca
     2821 tcgccatatt ctgaccgctg cccgaaaagc tgaagccgga aacggtaatg atgttttcta
     2881 tttcaggaat gcttttcgcc agttgggtaa cttgcgccaa agtcgcattg gtgcgctctt
     2941 gggtcgctcc tgcaggcagt tgcacgctga ccatgacgaa gccttggtct tcggtcggca
     3001 ggaatgaagt cggcaggcgc ataaacagga acacgcccac aaccgccaag ccgatataga
     3061 caaccatcat gcggaaagtc ttacgcagca ctttggcaac ccggccttcg taaccgtgcg
     3121 tccaactgtt gaatttcttg ttaaaccagc cgaagaaacc ttttttctct tcgtgatgcc
     3181 ctttcgggat tgtcttcaac attgtggcac acaaagcagg ggtaagggtc agcgcaagga
     3241 aggcggagaa tgcgattgat gacgccatcg tcagggcaaa ctgtttgtaa atattgcccg
     3301 tcgccccgct gaacatcgcc aacggtacga acacggaaat cagaacggcg gtaataccga
     3361 tgaccgcgcc cgaaatctga cccatcgctt ttttggtcgc ttctttgggc ggcaagcctt
     3421 cacccgccat aatgcgctcg acgttttcaa ccaccacaat cgcgtcatcg accacgatgc
     3481 cgatgaccaa aaccatcgca aacatggtca gtacgttaat cgacatgccc atataagaga
     3541 tgaaggcgaa accgcccaac agcgaaatcg gtacgacgat ggtcggaatc agcgtataac
     3601 ggatgttttg caggaagaga tacattacga caaacaccag caccatcgct tcgattaaag
     3661 tgtgaatcac tttttcaatc gaaatttcga cgaatttgga agtatcgtaa ggggttttcc
     3721 agctcatacc ctgaggaaag tatttttcca acgtcgccat gcgttcttta accgcctttg
     3781 ccgtcgccat cgcattgccg ctgttggaca gcatcaccgc cataccggtg gtatttacac
     3841 cgttcagacg ggttgaggaa gaatagtctt ccatacccag tccgaccctt gccacatcct
     3901 tcaggtaaac attagaaccg tcggtattgg cgcggaggat gacgttgccg aattcttctg
     3961 ccgtacccaa ctgcccttgc gccgttacgg tagccgtaac cgtctgtccg cgaacggcgg
     4021 gaagcgaacc gatagaaccc gctgaaatct ggacgttctg ggcggacagc gcgctgccaa
     4081 catcggcaaa cgacaaattg tagttttgca gtttcttagg atcaacccaa atccgcatcg
     4141 cgcgttgcgc gccgaacagg cgtacctgcc ccacgccttc gatacgctgc aactcgggaa
     4201 cgatattacg ctgcgcgtag tcgttcatct cttcggttga ctgcacatcc gacgaaagca
     4261 tcacaatcat caggaaattg gaacgcgcct tggatacggt tacgccgtat tgctggacag
     4321 ttgccggcag cgtgctcaat acttcggaaa gcttgttctg cacttccacc tgcgccagat
     4381 tctcgtcggt atcgggcgta aaggtcaggc tcacgctgcc gctgccgctc gaatcggcgg
     4441 aagtggacat ataatccaaa ccttccacgc cgttcatatt ccgctcgatc acggaaagca
     4501 cgctgtcttc cattacctgc gcggacgcgc ccggataagt ggccctcagg gtgatggtcg
     4561 gggcggcgac ggacggatat tgcgaaaccg gcaggctttt gatgccgaaa atacccgccg
     4621 caataatgaa aatcgaaata acccacgcaa aaatggggcg gtcgataaaa aatttagcca
     4681 tcgatgcctt ccttatttcg cttcagaagc aggtttggct tcagatgccg tctgaacgcc
     4741 ggattgaggc gcggcggctt ggttttcaga cgacgcccat tctttgggcg ttaccttttt
     4801 cgcacccgtt ataccggcga tactgatgcc ttccacaacc accttgtccc cgtccttcag
     4861 acccgacgta acaatccaat tcgtaccctg ctgttgcgca accgttacct cgcggggttc
     4921 cataccgcct tgggcattca caatcatcac ggtatctttc gcaccgcgcg ttaccgcctg
     4981 ctgcggcaca acaaatgcgt tatccaccgc cacttggtcc atcagcacgc gcacatacag
     5041 accgggcatc aagatattct gatcgttcgg tacggcggcg cgcagggtaa tctgaccggt
     5101 cgattcgttg acggccggat cggcaaacag caggcggcct ttttcagggt aaactgtgcc
     5161 gtcgtcaaat ttgatgccga ccgcaatcac accatccgcc gccagcagtt tgccttcggc
     5221 tatctgacgg cgcaatttca tcacttcgga tgcagactgg gtaacgttca catacatcgg
     5281 attggtttgg cggatggtcg ccagtacggt cgcatcgcca gcgttcagca acgtaccttc
     5341 ggaaactttg gactgaccga taaagccgga aatcggcgcg gtaatgcgcg aacggttcag
     5401 gctgatgccg gcggatttga ttgccgcctg cgccgcttta acgcctgcct cggcagaacg
     5461 tttcgccgtt accgcagcat cgtattcctg ccggctgacg gcttcggcgg caaccaaagg
     5521 cttgtatcgc gccaaatccg catccgcttt ggcaagcgtt gcctgagccg ttgccagttg
     5581 cgcgcgcgcg ctttccagac ctgcttcata agtggaactg tcgatctgat acagcggctg
     5641 tccggcacgg acataactgc cttcttggaa caggcgtttt tggatgatgc cgccgacttg
     5701 ggcgcggaca tcggcggtac gcagcgattc caaacgcccc ggcaactcga cggtcaatgc
     5761 gacggtttgc ggatggacgg ttacgacacc gacgacgggc gcaggggctt cccgaccagc
     5821 aggctgcccg ccctgcgccg cgtctccgcc tttaccgcaa gacgacagta ccaatgcaac
     5881 ggcggcagcc aacgcggccg cacgcatcgc cttaaaagca taaaaagcca ttatttatcc
     5941 tatctgtctg gtttgatgta aagggttttg ccaatcaaca ggcat
//
LOCUS       DAWGRZ010000130         5934 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000130 DAWGRZ010000000
VERSION     DAWGRZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.130"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            59..1597
                     /gene="murJ"
                     /locus_tag="WHT44_001608"
     CDS             59..1597
                     /gene="murJ"
                     /locus_tag="WHT44_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ2533771.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPS
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLASGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLWAAQACLPFEWVRAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            1730..2686
                     /locus_tag="WHT44_001609"
     CDS             1730..2686
                     /locus_tag="WHT44_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HEZ2533772.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPAYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSGSHYAVELMLAVRDRGWNGAVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGERGENALPQAAA
                     VISAPVDAEAAGRRFDSGITRLLYTRYFLRTLIPKARSLHGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPTPDEVSEAV
                     TLFQPAYGGHVGFVGSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            2737..3519
                     /gene="trpC"
                     /locus_tag="WHT44_001610"
     CDS             2737..3519
                     /gene="trpC"
                     /locus_tag="WHT44_001610"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ2533773.1"
                     /translation="MTDILNKILTAKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIGS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHDESELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRADNIEAEVGKLF"
     gene            3585..5885
                     /gene="recQ"
                     /locus_tag="WHT44_001611"
     CDS             3585..5885
                     /gene="recQ"
                     /locus_tag="WHT44_001611"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ2533774.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFISSFDRPNIYYQVIEKNNGKK
                     QLLEFIRKEMAGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEESEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGAELSDKEWRSVIRQCISLGYLTVNIARYQALQLTEAAKKVLKGETEVMLRPLK
                     RGKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QNLNGLHDIYGLGEAKTERFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLADTPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDETAKHKRRLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
BASE COUNT         1293 a         1758 c         1619 g         1264 t
ORIGIN      
        1 tatcaaagtc tgatcctgcc gccctaaagg gcggggtttc aaccgaaaag gaaatacgat
       61 gaatatgctg ggagctttgg caaaagtcgg cagcctgacg atggtgtcgc gcgttttggg
      121 atttgtgcgc gatacggtca ttgcgcgcgc attcggcgcg ggcatggcga cggatgcgtt
      181 ttttgtcgcg ttcaaactgc ccaacctgct tcgccgcgtg tttgcggagg gggcgtttgc
      241 ccaagcgttt gtgccgattt tggcggaata taaggaaacg cgttctaaag aggcgacgga
      301 ggcttttatc cgccatgtgg cggggatgct gtcgtttgtg ctggtcatcg ttaccgcgct
      361 gggcatactt gccgcgcctt gggtgattta tgtttccgca cccggttttg ccaaagatgc
      421 cgacaaattt cagctctcca tcgatttgct gcggattacg ttcccctata tcttattgat
      481 ttcactttcc tcttttgtcg gctcggtact caattcctat cataaattca gcattccggc
      541 gtttacgccc acgtttctga acgtgtcgtt tatcgtattc gcgctgtttt tcgtgccata
      601 tttcgatcct cccgttaccg cactggcatg ggcggttttt gtcggcggca ttttacaact
      661 cggcttccaa ctgccctggc tggcgaaact gggttttttg aaactgccca aactgaattt
      721 caaagatgcg gcggtcaacc gcgtcatgaa acaaatggca cctgcgattt tgggcgtgag
      781 cgtggcgcag atttctttgg tgatcaacac gattttcgcg tcttatctgc aatcgggcag
      841 cgtttcatgg atgtattacg ccgaccgcat gatggagctg cccagcggcg tgctgggggc
      901 ggcactcggt acgattttgc tgccgacttt gtccaaacac tcggcaaacc aagatacgga
      961 acagttttcc gccctgctcg actggggttt gcgcctgtgt atgctgctga cgctgccggc
     1021 ggcggtcgga ctggcggtgt tgtcgttccc gctggtggca accttgttca tgtaccgcga
     1081 attcacgctg tttgacgcgc agatgacgca acatgcgctg attgcctatt ctttcggttt
     1141 aatcggtttg atcatgatta aagtgttggc atccggcttt tatgcgcggc aaaacatcaa
     1201 aacgcccgtc aaaatcgcca tcttcacgct catctgcacg cagttgatga accttgcctt
     1261 tatcggccca ctgaaacacg tcggactttc gcttgccatc ggtctgggcg cgtgtatcaa
     1321 tgccggattg ttgttttacc tgttgcgcag acacggtatt taccaacctg gcaagggttg
     1381 ggcggcgttc ttagcaaaaa tgctgctctc gctcgccgta atgggcggcg gactgtgggc
     1441 ggcgcaggct tgcctgccgt ttgaatgggt acgcgccggc ggaatgcgga aagcggggca
     1501 gctctgcatc ctgattgccg tcggcggcgg actgtatttc gcatctctgg cggctttggg
     1561 cttccgtccg cgccatttca aacgggtgga aagctgaccg atgccgatat cctttttcgg
     1621 aaaacgcttt ttccgcgcct atattcgggc gcgcatcccc cacgtccgca ccagccggac
     1681 gggctgccgc atcatcaatg ccgtctgaaa acaagaaaac cgatacctta tgattttaac
     1741 accgccggac acgccctttt tcctccgcaa cggcaatgcc gacacgattg ccgccaaatt
     1801 cctgcaacgc cccgcacctg cataccgccg cgagctgctt ccagacagca cgggtaaaac
     1861 caaagtcgcc tacgactttt cagacggcat ttcgcccgac gcaccgctgg tcgtgctgtt
     1921 tcacggtttg gaaggaagca gtggcagcca ttacgcggtc gaactgatgc tcgcggtacg
     1981 cgatcgcggt tggaacggcg cagtcgtcca tttccgcagc tgcggcggca ttgccaacac
     2041 cgctccggtg ttctaccact tgggcgatac cgccgaaatc gcctttactt tggacacgct
     2101 tgccgcgcgt taccgtgaaa tatacgccgt cggcgtatcg ctgggcggca acgcgctggc
     2161 aaaatatttg ggcgaacggg gcgaaaacgc gctgccgcaa gccgctgccg tcatctccgc
     2221 acccgtcgat gcagaggcgg caggcagacg cttcgacagc ggcatcacgc ggctgctcta
     2281 cacgcgctac ttcctccgca ccctgatacc caaagcacgg tcgctccacg gttttcagac
     2341 ggcatttgcc gcagggtgca aaacactggg cgagtttgac gaccgcttca ccgcaccgct
     2401 gcacggcttt gccgaccggc acgactacta ccgccaaact tcctgcaaac cgctgctcaa
     2461 acacgttgcc aaaccgctgc tcctgctcaa tgccgccaac gaccccttcc tgccgcccga
     2521 agcgctgccg acaccggacg aagtgtccga agccgttacc ctgttccagc cggcatatgg
     2581 tggtcatgtc ggctttgtcg gcagcaccgg cggcaggctg cacctgcaat ggctgccgca
     2641 gaccgtcctg tcctatttcg acagcttccg cacaaacagg cggtaacggt ttgatgctaa
     2701 tattccccct ttccccggac aaatacggaa cacgacatga ccgacatcct caataaaatc
     2761 cttaccgcca aggcacagga agttgccgct caaaaagccg ccgtcaacgc cgaacacatc
     2821 cgcgcacttg ccgcagaagc cgcgcccgtc cgcagcttca tcggttcgat acgcggcaaa
     2881 caccgcctaa acctgcccgc cgtcattgcc gaaatcaaaa aggcaagccc gagcaaaggc
     2941 ctgatccgtc cggacttccg accggccgag attgcacgcg cctatgaaaa cgccggggcg
     3001 gcgtgtttgt ccgtactgac cgacgaaccc tatttccaag gttcgcccga atacctcaaa
     3061 caggcgcgcg aagccgtatc gctgcccgtg ctgcgcaaag acttcatcat cgacgaatat
     3121 caggtttatc aggcgcgcgc atggggggcg gatgccgtcc tgctgattgc cgcagcactg
     3181 gaacaggaac aattggaacg ctttgaagcg gtggcgcacg aattgggcat gaccgtcctg
     3241 ctcgagctgc acgatgaaag cgaattggaa aaatgccgca acctgaccac gccgttgtgg
     3301 ggtgtaaaca accgcaacct gaggactttt gaagtctccc tcgaccaaac cctgtcgctg
     3361 ctgcccgcgc tggaaggcaa aaccgtcgtt accgaaagcg gcattacagg caaggcggat
     3421 gtggaattta tgcaatcgcg cggcgtgcat accttcctga tcggcgaaac gtttatgcgt
     3481 gccgacaata ttgaggcgga agtaggcaaa ctcttctaaa tcccgatttc agacggcatt
     3541 ccctttcttt aaccgacaca ccctttaccg gacacggaac catcatgcac cgaccgaccg
     3601 ccaaacagat tctgcacgaa gtatttggtt atcccgaatt tcgcggcagg caggaggctg
     3661 tcatcaatac tttggcaggc ggcgggagtt tgacggtgct gatgccgacg ggcgggggca
     3721 agtctttgtg ttaccagatt cccgcgctga tgcgcgaagg cgtggcggtt gtcgtatcgc
     3781 cgctgattgc gctgatgaac gaccaagtgg ccagcctgca tgtggccggg attgaagcag
     3841 cggcagtcaa cagcggcaca tcggcagatg aggcgcgcga gattgccgac aagcttgccc
     3901 aaggccgtct gaaactgctt tatgtcgcgc cggaacgctt ggttaccgat cgctttttgc
     3961 gttttctcga ccaacaaacc gtcagcctgt ttgccattga tgaggcgcat tgcgtcagcc
     4021 agtggggaca cgatttccgc cccgaatacc aacagctcgg catgcttgcc gaacgctatc
     4081 cgaacgtccc gcgcatcgcc ctgaccgcca ccgccgatgc cgccacgcgc gccgacatca
     4141 agcattatct gcacttggac gatgcgcccg aatttatttc cagcttcgac cgcccgaata
     4201 tttattatca ggttatcgaa aaaaacaacg gcaaaaaaca actgcttgaa tttatccgca
     4261 aagaaatggc ggggcaaagc ggcattgtgt attgcctaag ccgcaaaaag gttgaagatg
     4321 tggcgcagtt tttgcgtgaa aacggattaa acgcgattcc gtatcatgct ggtttgagca
     4381 tggacgtgcg cgaggaaaac caacgccgct ttacgcatga agacaatatt atcgtggtgg
     4441 cgaccgtggc gttcggcatg ggcatagaca aacccgacgt gcgctttgtc gcccatctcg
     4501 atatgcccca gagtgtcgaa catttctatc aggaatcggg gcgtgccggc agggacgggc
     4561 tgcctgccgc gagctggctg tgttacggct tgaacgattg ggtgttgctg cgcgaacgga
     4621 ttgccgaagg caacagcgat gaggtgcaaa agcaaatcga aatgcaaaaa ctcgatgcca
     4681 tgctttccgt ctgcgaaacc gccgcctgcc gccgcgtact gttgctcaaa cattttggcg
     4741 aagaatccga accctgcggc cattgcgaca actgcctgca tccgcctgtg cggtttgacg
     4801 gcacggtgtt ggtacaaaaa ttactcagct gcgtgtaccg cgccggacaa cgttttgccg
     4861 ccggttacat caccaacctt ttgcgcggta aaagcgacga ttggatacgc ggcaaccggc
     4921 acgaacaact gtctaccttc ggcatcggtg cggagttgtc cgacaaagaa tggcgcagcg
     4981 tcatccgcca gtgcatcagc ctcggctacc tcaccgtcaa cattgcccga tatcaggcat
     5041 tgcaactgac cgaagccgcc aaaaaagtcc tcaaaggcga aaccgaagtg atgctgcgtc
     5101 cgctcaagcg cggcaagccc gccgcccgca ccctcaaaga caactggctg cgtaccgaac
     5161 gcgaagaacg cctgtggcag gcattgcgcg tttggcgtat gaaacaggca gaagccgaag
     5221 gcatccccgc ctatatgatt ttcggcgaca aaaccctgcg cgaccttgtc gaaaaaatgc
     5281 cgcaaaacct caacgggctg cacgacatct acggcttggg cgaagccaaa accgaacgtt
     5341 tcggacacgg catactcgaa gtctgccgca acgccgccgg cttcagccgc gacgcggtca
     5401 tccgtccgca aaccgaacgc gaacaacaac tgcgtcaaaa actcgaggcc tggcggtatg
     5461 aacaggcaag ggcggaaaac tgcgccctgc atgccgtcct ctccgacgaa agccttgccg
     5521 atatgcttgc cgatacgccc gaaaccgaaa ccgacctcga aggcgtacac ggcttgggca
     5581 gcgtacgcgc cgccaaatac ggacgggaca tcctcgccgt ctgccgtccg ttttcagacg
     5641 gcatcgatga aaccgccaaa cacaaacgcc gcctgatgcg cgccctgatc caatggtgca
     5701 acgaaacggc aaaacacgaa cagtccgaac cctaccgcat tctcagcaaa gccgccctac
     5761 gcgccattgc cgccaaacag ccggaaggtt tggcggagct tgccgccgta tatggcgtag
     5821 gcgaagaaaa agccgcacgt tacggtgcgg cggtgttggc ggtattggaa cgaaatgccg
     5881 tctgaaaacc gttaccaagt ttcagacggc attgcctcta tttaaaaatt cctg
//
LOCUS       DAWGRZ010000131         5924 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000131 DAWGRZ010000000
VERSION     DAWGRZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.131"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..362)
                     /gene="mafB"
                     /locus_tag="WHT44_001612"
     CDS             complement(<1..362)
                     /gene="mafB"
                     /locus_tag="WHT44_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ2533775.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNARGSVKNRVYAVQTFDATAVGPILPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDNHDSKSTSDFSGG"
     gene            complement(402..1364)
                     /gene="mafA"
                     /locus_tag="WHT44_001613"
     CDS             complement(402..1364)
                     /gene="mafA"
                     /locus_tag="WHT44_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ2533776.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPARSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVEADNSHEGYGYSNEAVRQHRQGQP"
     gene            complement(1548..2267)
                     /locus_tag="WHT44_001614"
     CDS             complement(1548..2267)
                     /locus_tag="WHT44_001614"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ2533777.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            complement(2484..3338)
                     /locus_tag="WHT44_001615"
     CDS             complement(2484..3338)
                     /locus_tag="WHT44_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ2533778.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(3467..4195)
                     /gene="rpsB"
                     /locus_tag="WHT44_001616"
     CDS             complement(3467..4195)
                     /gene="rpsB"
                     /locus_tag="WHT44_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ2533779.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(4368..4601)
                     /locus_tag="WHT44_001617"
     CDS             complement(4368..4601)
                     /locus_tag="WHT44_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533780.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFT
                     ITVRRRNHLMRQQEQAESEQQRAQRQKDSGTKP"
     gene            4679..5260
                     /locus_tag="WHT44_001618"
     CDS             4679..5260
                     /locus_tag="WHT44_001618"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ2533781.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAATVKINHLLKRYIKKGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAELYLPYIEPRSRRMWFTPYPADGVKQERKRGR
                     AKLHVPQFAGRKKRVHDLNLLLVPVVGMDRLGYRLGQAGGYYDATLSAMKYRLQAKTV
                     GVGFACQLVDRLPVEAHDRSLDGFVSEAGILCF"
     gene            5321..5911
                     /locus_tag="WHT44_001619"
     CDS             5321..5911
                     /locus_tag="WHT44_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ2533782.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDSAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT         1326 a         1356 c         1631 g         1611 t
ORIGIN      
        1 ccgccgctga aatcagaagt gctttttgaa tcatgattat cgaacggact gtgtacttca
       61 tgtccatgtc ctgaaaaatg ggtttcataa ccgataatgc cttcaaatcc tgtccgttcg
      121 tgtgtaatag gcagtatggg gccgaccgca gttgcatcaa atgtttggac ggcgtaaacc
      181 cgatttttaa cactgccgcg agcattacca aacagatggt atttaccacc cggttcgtag
      241 tgttttgcct gcatatcatc gcgcaaacgg gcatccaaac cgttcgcatg actaatgggg
      301 atttgcagca acgatattgc tgctgcaaac agcatcatga atttttgaat aggcaaattc
      361 attttgtttt ccttggtagc aagcggttat ggtagtgtga atcaaggttg cccttgtcta
      421 tgttgtcgca ctgcttcatt gctgtatcca tacccctcat gactgttatc agcctctacg
      481 gatggggcgg agttacccgt atgattgccg tatggtcgga tatcggagaa atcgaccatt
      541 aatccttccg tcggtttaat tcctttgctt actttatacg gccccatcca caatgcgtaa
      601 ttttctttat aggcagcttc aaacgcattg gtttttggtt tgatgagcaa ttttttattg
      661 gttctgtcta ctgcgaaata ttccagtttt gtttgggctt tcagtgtttc ggcattgtat
      721 aggtgcattt cggttctgtt gcgtatcgtt ccgaatacgt cgatgttaat aaacacatcc
      781 gtatcggcat tggcaggaga aacaacgtct atgccgcgca ggaaaaatac ggtttgcacc
      841 aagtgggaaa gaaaggcagt gtcgcgcggg ttagtcgtca aggtttcatt tcgataatcc
      901 cccatcccgc caatatttaa gcccagactg ctcctacttc cgctaccgtc tgattgggtg
      961 cgcgagcgtg caggggcatt aagtgtagat aaagaagtgg ttaaacctgt caaaccgcct
     1021 gatgttgttt cagcggtggt ttcgtaacgt ggataggtgt aatcggtacg gacggcaggg
     1081 ctgtttatgt attcgccacg aatcagtgca tcaatggagt agcgacctcc tgtcaaactg
     1141 cctgaacctt ggtcgcccat agtggcaatg tacaatgcaa cttttcgtcc gtgtaatgcc
     1201 tgtaaatcca tgtctttaac ggcagctctg gcagaagcgg ccacaagttc ttgttcgacc
     1261 gcaaagcgtt tacctccgcc atgcgatgga atacctgtca gtgtcccgca ggcggataaa
     1321 ataaaaactg aaaaaagaat aggtatcagc agccgtgctt gcatagattt tctcctttga
     1381 tgaaaaacaa attgtatcaa aattgtaaat atagtggatt aaatttaaac cagtacggca
     1441 aggcgaggca acgccgtact ggtttaaatt taatccacta taatctccat ataataaatg
     1501 gctgtctgaa gcccatatgc gactatatct gccgacacta tggtcaatca gcagtgaacc
     1561 agcgtacctt cgttttcacc ggttactaca cgtttgagcg agccttcttt agcaatgccg
     1621 aatactacaa tattgagctt gcgttcgcgg cagagggcga aagcggtggc atccatcact
     1681 ttgaggtttt tcaacaaggc ttcgtcaaaa gtaatggttt cgtagcgcgt ggcggatgag
     1741 tcttttttgg ggtctgcggt gtacacgccg tcaacgttgg tggctttgag catcacgtcg
     1801 cagttcattt ccgcaccgcg caatgcggcg gcagtgtcgg tcgtgaagaa cgggttaccg
     1861 gtaccggcgg cgaaaatcac gactttgcct tcttccaaat attggatggc tttggggcgg
     1921 gcgtaggttt cagcgatttg ctgcatagac agtgcggatt gtacgcgcgc tttgatgcct
     1981 aaagtttcaa atgcgtcttt gagtgccaac gcgttcatca cggttgccat catgcccatg
     2041 tagtcggcgg tggcgcgatc catgctgcct gcttgggcgg atacgccccg gaaaatattg
     2101 ccgccgccga caacaatacc gacttgcacg cccattttaa cgacttcggc aatttcgccg
     2161 acagtttgaa cgatggtatc gtgattgatg ccgaacggat cggaacccat cagggattcg
     2221 ccggagagtt tcagtaatac gcgtttgtat ttgatttgct gtgtcatgga taccttgctt
     2281 tcttgaatgt cgtcggtcgg agttgggtcg tctgaatttc aaacggtcgg aatattaatg
     2341 atagggtgtg tcggtttatt attgttttgt tttaagacgg tctgaatatt cggtcgtctt
     2401 aaaataggta ccgcaaacgg ttttctcaaa aaaaagcacc ctgattcgtt tggaatccag
     2461 gtgctttctt tttcataagt gccttacact ttggcggctg cggctacttc ggctgcgtag
     2521 tcgacaacgg ctttctcgat accgtcgcct actttgtagc ggacgaagct gaccacttca
     2581 gtgccgtttt ctttggcgaa ttgggcaaca gtttgatcgg ggttcatcac gaatgcttgg
     2641 ccgttcagag tgatttcagc caagaatttg cgaatacggc cttcaaccat tttagcggcg
     2701 atgtcggcag gtttgccgga agcgatggct tgctcggtgt agatgtggcg ttctttttca
     2761 acggtttcgg catctacttc ggcttcgctt acgcattgtg gtttagcggc aacaatatgc
     2821 ataccgattt tgcgtgctac gtcttcagag cctttgtact caaccaatac gccttcggtc
     2881 gccaatgcgc cgtggatgta ggcaaccagt tggttggcag tgtcgatcac ttggaagcga
     2941 cggacagaca tattctcgcc caatttggcg atgatggctt tgcgttctgc ttcaaccagt
     3001 tcgctcagtt cttcaacaga agccggtttt ttctcggcag cagttttcgc aacgaagttg
     3061 gcaaattcta cgaagcccgc gtctttagca acgaagtcgg tttcgcagtt tacttcgacc
     3121 aatgcgccga cattgccgtt gatcgcgtaa gccaatacgc cttcggcagc ggtacggccg
     3181 gccagtttac cggctttcgc accggatttg atacgcagga tttcttcggc tttgtcgaag
     3241 ttgccttcgg cttcaaccaa ggcttttttg cattccatca tgcccaggcc ggtagcggcg
     3301 cgcaggtcgg caaccatttt tgcagtaatt tctgccattt tgaatctcct agatttttgg
     3361 gaaatacgga aaggattttc cgcattcggg aatcgtgtgc cgcgaacgga cgttcagacg
     3421 gcatatttga gaaaaggggc ataacgcccc tcttcggttt gccggattac tcggcagcgg
     3481 cttcttgggc agcggctacg gtttcttgca gcgcttggtt tttgccttcc aaaactgcgt
     3541 cagcgatgcc gcggcagtac aggcggatgg ctttggcgga gtcgtcgttg ccggggataa
     3601 cgtatttcac gccgtcgggg ctgttgttgg tatcgactac ggcgataaca gggatgccca
     3661 atttttcagc ttcaaccaga gtaccttttt ggtagccggt atcgataacg aaaatcgcgt
     3721 caggcaggcc tttcatgttt ttgataccgc ccaaagaacg ttccagtttt tcaacatcgc
     3781 gttgcatttc cagaatttct tttttgctga aaccgctttc ggcagcattt tccaaggctg
     3841 cggttttttc ttccaggcgt ttgatggatt gcttaacggt tttgtagttg gtcagcatac
     3901 cgcccaacca gcggtaatcg acgaaaggca taccggcgcg ggtcgcttct tcgcggatga
     3961 tgtcgcgggc ttggcgtttg gtacctacga acaatactgt acctttgttg gcaaccagac
     4021 gacgtacggc ttcttgcgcg tcttggaaca tcggcagggt tttttccagg ttgacgatat
     4081 ggattttgtt gcgcgcaccg aaaatgtatt gtgccatttt cgggttccag aaacgggttt
     4141 ggtggccgaa gtgaacaccg gcttcaatca tctgacgcat agtaatttga gacattttat
     4201 ttccttgaaa gggttaagag cacacattca atcgcataga acttgatagg tcacgcctat
     4261 tgtcaagcac ccttcggcga aagtgggcat aagttttaaa aaatagtgtg tttccgaaat
     4321 ggaaaactga cggattatag cggataagat gccgtctgaa aagggattca gggttttgtg
     4381 ccgctgtctt tttgccgttg tgcgcgctgc tgttcggatt ccgcctgttc ctgctgcctc
     4441 atcaggtggt tgcggcggcg gacggtaatg gtgaaaaccg tgaacacggt gggcagcacc
     4501 gcccaaaaaa ccaaataaat caaggcgcgc gcaatactcg gctgcgcggc ggaatacata
     4561 acggcaacaa aaagatagcc gatggcgacg atgtgccaca ttgtgtgcgt cctgtttgaa
     4621 tgttaacaaa atcgttataa tcgcaacatt ctaccgcaat cctgaaaggc cgtctgaaat
     4681 gaggaacgag gaaaaacgcg ccctgcgccg cgaattgcgc gggcggcgtt cgcaaatggg
     4741 gcgggacgtg cgggcggcgg caacggtaaa aatcaaccac ctgctcaaac gttatattaa
     4801 aaaagggcgg aaaatcggcg tgtattggcc gatgggcaag gaattgcgtt tggacggctt
     4861 tgtccgcgcg gcgcaaaaac gcggtgcgga actctacctg ccttatatcg aaccgcgttc
     4921 gcggcggatg tggtttacgc cgtatcctgc cgatggagta aaacaagaac gcaagcgcgg
     4981 tagggcgaag ctgcatgtcc ctcagtttgc aggtcggaaa aagcgtgtgc atgatttgaa
     5041 cctcctgctt gtgccagtgg tcggtatgga caggctgggc taccgcttgg gacaggcagg
     5101 cggctattac gatgcgacgc tttcagcgat gaaataccgt ttgcaggcaa aaaccgtggg
     5161 cgtgggcttt gcctgccagt tggtggacag gctgccggtc gaggcgcacg accggtcttt
     5221 ggacggtttt gtgtcggagg cggggatatt gtgtttttag ccgttcagac ggcattccgg
     5281 gcggaaatgc cgtctgaagg cggaatattg ggagaaaagg atgtcggaaa agataatact
     5341 gccggtcttg tcgttgggcg gcgttcgtct cgaaccgttg gatgtgcatc atgaaacggg
     5401 tttgcgcgag gcggtttgcg atggggaggt ttggaagctg ggggtaacgt ccgcgcccca
     5461 tccggatagg gtggcagact atatcgggac agcattggca acgcgtttgg cgtttgcggt
     5521 tgtcgatgaa gaggcgggca gggtggtcgg gacaacggcg tattatcatt ttgaaccgca
     5581 gattccgcgt ttggatatcg gattcacatg gtatgcggca tcggcacggc ggacacggat
     5641 aaacacctgt tgcaaaatca tgctgttgga ttccgcattt gatgttttgg cttgccgttg
     5701 cgtgggatgg cgcacggata ttctgaacct tgcctcgcaa cgtgccatcg aacggctggg
     5761 tgcggaaaaa gacggcgtgc tgcgtatgca tatgctccgt aaagacggca gtgtgcgcga
     5821 tacggtcgtg tacagtatgc tgagggaaga ttggtgcaaa aacagggaaa tcctgaccgg
     5881 caggttggca gggtatggcg tgcaggtgtg aggcagtatt ttca
//
LOCUS       DAWGRZ010000132         5883 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000132 DAWGRZ010000000
VERSION     DAWGRZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.132"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            131..565
                     /locus_tag="WHT44_001620"
     CDS             131..565
                     /locus_tag="WHT44_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ2533783.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQSQHNELRKIRTAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(578..1441)
                     /locus_tag="WHT44_001621"
     CDS             complement(578..1441)
                     /locus_tag="WHT44_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ2533784.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(1449..2066)
                     /locus_tag="WHT44_001622"
     CDS             complement(1449..2066)
                     /locus_tag="WHT44_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ2533785.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFHTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(2229..2842)
                     /locus_tag="WHT44_001623"
                     /pseudo
     CDS             complement(2229..2842)
                     /locus_tag="WHT44_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
     gene            3048..4820
                     /locus_tag="WHT44_001624"
     CDS             3048..4820
                     /locus_tag="WHT44_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ2533786.1"
                     /translation="MILPARFSDGISLSLRLKLLTGLWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRAQIDNQVAEFEKSLKRIAQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENISFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQAQQGTTVSLTVASEESLK"
     gene            4817..5473
                     /locus_tag="WHT44_001625"
     CDS             4817..5473
                     /locus_tag="WHT44_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ2533787.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVPE
                     "
BASE COUNT         1444 a         1655 c         1364 g         1420 t
ORIGIN      
        1 ttccgattcc gccgttatgt ctgcctgccg gacatacgta cgcattatta acaaaagtta
       61 accgcgataa taccatcttt cacacgtcaa tctagtatat ttcctaaaat ttccaacaag
      121 aggaaaagcc gtgccactgc ctgctccctg ccgttttgcc aaacctgccg cctctttttt
      181 aagtatggct ttgctttcct gtcagctttc ccacgccgcc acggcttata tccccccgaa
      241 cgattttcaa ccgaactgcg acatacgccg actcgggctg acccaaagtc agcacaatga
      301 gctgcgtaaa atccgcaccg ccttcaaaat ggcgggcgac agggcgcgtt tgaaggttat
      361 gcattccgaa cacagccgcc gccggtctgt cgtcgaaatc atttcctcgg atgtttttaa
      421 tcggaacgag gcgcgcgatt atgtcgaaag ccgctatttg tccggtatgg attttgcggt
      481 ggacgaattg gaaatccaac accggttctt ccatatcctc acaccgcaac agcagcaaat
      541 gtggctttct tcctgcctca aataatcccc gaaacgctca caacgcccgt tgtttcggca
      601 gcctgcccgc ccagtcgcag gcaaactgcc acgcggaacg tcccgaacgg ctgccccgcg
      661 tctgcgccca ctgcaatgcc gccatctgtg cggtttcatc atacggcacg ccgaaatctt
      721 ccagccaatt ttgcaccgcc gccagataat cgttttgatc gaacggataa aaactgagcc
      781 acaatccgaa tcggtcggac agggatattt tttcttccac cgcttctttc tgatggattt
      841 ccccccgcat ccccgtcgta ccggcattct cgtcaaaata ttcgggcatc aggtgccgtc
      901 tgttggaagt cgcgtaaacc aaaacgttgg cgcaacgttg agacagaccg ccgtctaacg
      961 cggttttcaa tgccttatag gtttcatcgc cgctttcaaa cgacaaatcg tcgcaaaata
     1021 cgataaattt ttccggacgt tccttcaaaa gcgtcaacag gtaaggcagg ccgattaaat
     1081 cgcttttatc gacttcgatc aggcgcaatc ccttatccgc atattcgtgt agcagggctt
     1141 tgaccagcga ggatttgcct gttccgcgcg cgccgctcat caatacattg ttcgcgggtc
     1201 tgccgacaat gaactgttcg gtattacgca ccagcaattc ggtttgcctg ccgactcccg
     1261 ccagccttac caagggaaag gtgtgcggat cgggcaagtg ttccaaaaaa ccttttttgc
     1321 ccgcactctg ccagcggaag gcaagcgcgt tccaatccgt atgcccgggt tcgggcggaa
     1381 gcacggcatc caaacgccgc aaaacggcat aggctttatc gagaaattcg ttcaattcca
     1441 tctctgcctc actttgcata tctttgcgcc atcagccgtt cgacggtatc gacgattgcc
     1501 tgcgtattcg gatcgatttc gatgttgatc ctgtcgccga cctttctgct gccgaacagc
     1561 gtccgttcca aagtttcggg aatcagatgg acattgaaac ggccgtcttc gactttgcct
     1621 atggtcaggc tgcaaccgtc caagccgacg aaccctttgg tcaggatata gggtttgagt
     1681 tcatgcggga gcgaaaacca aaccgtgcgg ttgaacccgt cccgttcgat ttcgacaata
     1741 ggcacggttg ccataatgtg tccgctcatg acgtgtccgc cgatttcgtc tccgaaacgc
     1801 gccgcccgtt cgatgttgac gcaatcgcct tccttcagca gccccaaatt ggtttttgcc
     1861 aaagtttccg ccattaaatc gaaactgacg cggtttcctt cgatttcggt aatcgtcagg
     1921 cagcagccgt tattggcgac cgatgcgccg cgttgcagat tgtccgccgc ctcttgcgga
     1981 agctcgacga cataagtgtg aaatgcctcc gacgggcggt ggattgccgt cagttttccc
     2041 aatccttgaa caatgcctgt aaacataatc ctgtttccct gtgtcggtaa aaatggtgca
     2101 aattgtagca tctccccgcg aaaaatgccg tctgaaatgc cttcagacag cattatgcct
     2161 ccgattcggg caaaaaccgc ccggtatggc ttgacctttc ctttccacgc cggtcggcgg
     2221 tcttgccctt atccctcctg caaatcgatt tgcgtgttca agtcggcaaa atgcccgtca
     2281 aactcgaatc tgaccggccg cgccctttgc tgctgcaacc agcttcttaa tgttttcata
     2341 cccgaaaaca gatagggaat cgcgctttgc agaatctgcg ggcggatata cataatgttg
     2401 tagtgcatcg ttatcggcgt ttccacataa tacgcattgc acaacggtgt gcgtttcgac
     2461 acggtttcaa acctcgccac caaatcgtcc ggcagatacg gcatatcgca cggcacaacc
     2521 aaaagccagt cagcagccgc caactgcaaa tcgttggctg cggtacacaa tgccgaaagc
     2581 gggccgaaat gctgccactg ccgcgcatcg ggaaaaatat gcggacttct tcgagcatat
     2641 tcttccaaat tccggttggt gctgatggcg atatggctga cctgcggcct gaccctgtcg
     2701 atgacatggt ctatcagtgc cttaccccca aaagagcaag ccctttgtcc tcgcctccca
     2761 tacggctcgc ctgaccgccg gccagtatca gggcaaaagt tttcattgcg gatgttctct
     2821 tggaaaagtt cgaggttttc atgattgcag tctgccgttc ccaatgactc aaaatgccgt
     2881 ctgaagcaga cggcaaataa attcatatta tctgaatttt atcataacat gatttaacac
     2941 tgaagcggtg cggattaagt tttccttaac caattctttc tgagcagttg tatctaattc
     3001 caaagaatga tattgtttgc attatttgga acaatttttc gccgagcatg atactgccag
     3061 cccgtttttc agacggcatc agcctttccc tgcgcctgaa actcctgacc ggactgtggg
     3121 tcgggttggc ggcattgtct gtcgttttga cactgctgct ctctttgcgt ctggaaaacg
     3181 cggcctccgt catcgaagag gcgggcaact tgagaatgca ggcataccgt ctggcataca
     3241 tggcgggtga aggctcgccc cgtgcgcaaa ttgacaatca ggttgccgaa tttgaaaaaa
     3301 gtttaaaacg cattgcccaa agcgatgcca tccatccgct gattccttcg gacacccctc
     3361 ttgcttatga tttgatacaa tccatgctga ttatagattg gcaggcacac atcctccccc
     3421 cgctccagtc ctaccggcga ccgactcagg tcgatctcta ccgctttgcc ggaaacatcg
     3481 aactgttttt gcaggcattg gaaaatgcca acgaaaaaaa cacatggtgg ctcaggcgtt
     3541 ttcaatgggc aattatgttg atgacgctgg tgtcgtctgt actgatgctg ttttggcacc
     3601 agatttgggt tatccggccg ctgcaggcgt taagggaagg tgcggaacgc atcggacgga
     3661 ggtgtttcga tattccggtt cccgaaggcg gtacgccgga attcaaacag gtcgggcgtt
     3721 gtttcaatca aatgggcggc aggttgaaaa ttttatatga tgatttggaa ggacaagtcg
     3781 ccgagcagac acgcagtctc gaaaaacaaa atcaaaacct gaccctgctg taccaaacta
     3841 cacgggacct gcaccaatcc tacataccgc aacaggctgc agaacatttt ctaaaccgta
     3901 tcctgcccgc cgtaggagca gattccggca gagtttgttt ggacggcgga tccgatgttt
     3961 atgtttccat tcatcatgcg gattgcggca cagcagcttc ggatttgggg aagtaccatg
     4021 aggaaatctt ccccattgag taccagaacg aaacattggg caggctgttg ctcagctttc
     4081 caaacggcat ttctcttgat gaagacgacc gcatcctgct tcaaacacta ggcaggcaat
     4141 tgggcgtatc gcttgccggc gcaaaacagg aggaagaaaa acgcctgctt gcagtattgc
     4201 aggaacgcaa cctgattgcg caaggattac atgacagcat cgcacaagca ttaacgttcc
     4261 taaacctaca ggtacagatg ctggaaaccg cctttgccga aaacaaacgg gaggaagccg
     4321 cagaaaacat cagctttatc aaaacaggcg tgcaggaatg ttatgaagat gtccgcgaac
     4381 tgctgctcaa cttccgtacc aaaatcagca ataaagaatt tcccgaagcc gttgccgacc
     4441 tattcgcccg ctttacgcaa caaaccggga taacggtcga aaccgcctgg gaaaacggtt
     4501 cgttcctgcc gcctcaggaa gcgcagctcc aaatgatttt tatcctgcag gaaagcctgt
     4561 ccaacatccg caaacacgcc cgcgccaccc atgtaaaatt caccctttcc gaacacggcg
     4621 gacgctttac catgaccatc caagacaacg gacaaggttt cgacacggag aaaataggag
     4681 aacccacggg cagccatgtc ggactgcaca tcatgcagga gcgtgccaaa cgcatccatg
     4741 ccgttttaga aatccgttcc caagctcaac agggaaccac cgtctcattg acggttgcat
     4801 ctgaagaaag cttgaaatga ctattaaaat tattctgata gacgaccata ccctcttccg
     4861 cagcggcatt aaagcccttt tgtcgcgcca acacggtttt gaagtcatcg gcgaagccgc
     4921 agacggcctc tcgggtatca aaatgatcag tcggctgcaa cccgatgtcg tcctgcttga
     4981 ccttgatatg cccggtatga acggacgcga agcactctcc caaatcatca gcatcaatcc
     5041 gcagcaggca gtgattatgc tgaccgtttc cgaagacagc gacgatttga ccgaatgtat
     5101 gcgcatcggc gcgcgcggct acctgctgaa aaacatcaac gccgactttc tgctcgaaag
     5161 catacgcaaa gccgctgaag gcgataatgt attctcgccc gagatgaccg ccaaactcgt
     5221 caaaagcctg atttcccccc aacctgccca agggacgcag gcactctcct cacttacccc
     5281 tcgtgaactg gaaatcttgg gctatctcgc cgcaggacac agcaacaaaa tcatcgcccg
     5341 ccacctcgat cttgccgaat ccaccgtcaa agtccacgtt caaaacctgc tccgcaaact
     5401 caacctcagc agccgggtgc aggccgccgt ttacgccatc cggcacaacg tcccccaacc
     5461 tgtgccggaa taggcgttca gacggcatat taggggtttt aatccccgta cggtcattcg
     5521 gataacagac caagcatgta agtttatgcc cccataagta cgcttggcat agcagtaata
     5581 ttgttcggtt tagtgttttc cgtttgcccc tatctgatac tgcaatatca gctatgccgt
     5641 ctgaaaacgc atcatcatga tattttcaga cggcataata aaaagcggaa atactaatgc
     5701 agggtaaaat gttccattcc gaatcccata aatatacaat ggcttatatc gtttaagcat
     5761 gtgtaaccca ccctcatatc aatcaatata tagtggatta acaaaaaccg gtacggcgtt
     5821 gcctcgcctt gccgtactat ctgtactgtc tgcggcttcg tcgccttgtc ctgatttttg
     5881 tta
//
LOCUS       DAWGRZ010000133         5875 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000133 DAWGRZ010000000
VERSION     DAWGRZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.133"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(306..419)
                     /gene="rrf"
                     /locus_tag="WHT44_001626"
     rRNA            complement(306..419)
                     /gene="rrf"
                     /locus_tag="WHT44_001626"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(516..3407)
                     /locus_tag="WHT44_001627"
     rRNA            complement(516..3407)
                     /locus_tag="WHT44_001627"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3813..3888)
                     /locus_tag="WHT44_001628"
     tRNA            complement(3813..3888)
                     /locus_tag="WHT44_001628"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3853..3855),aa:Ala,seq:tgc)
     gene            complement(3894..3970)
                     /locus_tag="WHT44_001629"
     tRNA            complement(3894..3970)
                     /locus_tag="WHT44_001629"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3934..3936),aa:Ile,seq:gat)
     gene            complement(4072..5612)
                     /locus_tag="WHT44_001630"
     rRNA            complement(4072..5612)
                     /locus_tag="WHT44_001630"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1289 a         1709 c         1254 g         1623 t
ORIGIN      
        1 tgcgaagccg cattgatgtt taccgccgca agggagaagg cgcaggagaa cagcatacag
       61 aacagcacga acattttctt catggttttt cctttaaggg ttgcaaacaa taaaccgcat
      121 cttgcgacga taaaacgagt cattctaaaa tgaatatccc aaagtttcaa gccgttcctc
      181 cgcaaacccg accggacacc gtacggatgc cgtcccgcca tcaccgacat tttttccggg
      241 caaagcaaac attttttccg ggcaaagcaa aaacccccga ataatcgggg gttttctgaa
      301 tgggtgtttg gcagtgacct actttcgcat ggaagaacca cactatcatc ggcgctgagt
      361 cgtttcacgg tcctgttcgg gatgggaagg cgtgggacca actcgctatg gccgccaaac
      421 ttaaactgtt acaaatcggt aaagccttaa tcaatatatt cggtaatgac tgaatcagtc
      481 agtaagcttt tatctcttga agttcttcaa atgatagagt caagcctcac gagcaattag
      541 tatgggttag cttcacgcgt taccgcgctt ccacacccca cctatcaacg tcctggtctc
      601 gaacgactct ttagtgcggt taaaccgcaa gggaagtctc atcttcaggc gagtttcgcg
      661 cttagatgct ttcagcgctt atctcttccg aacttagcta cccggctatg caactggcgt
      721 tacaaccggt acaccagagg ttcgtccact ccggtcctct cgtactagga gcagcccccg
      781 tcaaacttcc aacgcccact gcagataggg accaaactgt ctcacgacgt tttaaaccca
      841 gctcacgtac cactttaaat ggcgaacagc catacccttg ggaccgacta cagccccagg
      901 atgtgatgag ccgacatcga ggtgccaaac tccgccgtcg atatgaactc ttgggcggaa
      961 tcagcctgtt atccccggag taccttttat ccgttgagcg atggcccttc catacagaac
     1021 caccggatca ctatgtcctg ctttcgcacc tgctcgactt gtcggtctcg cagttaagct
     1081 accttttgcc attgcactat cagtccgatt tccgaccgga cctaggtaac cttcgaactc
     1141 ctccgttacg ctttgggagg agaccgcccc agtcaaactg cctaccatgc acggtccccg
     1201 acccggatga cgggtctggg ttagaacctc aaagacacca gggtggtatt tcaaggacgg
     1261 ctccacagag actggcgtct ctgcttctaa gcctcccacc tatcctacac aagtgacttc
     1321 aaagtccaat gcaaagctac agtaaaggtt cacggggtct ttccgtctag cagcgggtag
     1381 attgcatctt cacaaccact tcaacttcgc tgagtctcag gaggagacag tgtggccatc
     1441 gttacgccat tcgtgcgggt cggaacttac ccgacaagga atttcgctac cttaggaccg
     1501 ttatagttac ggccgccgtt tactggggct tcgatccgat gctctcacat cttcaattaa
     1561 ccttccagca ccgggcaggc gtcacaccct atacgtccac tttcgtgtta gcagagtgct
     1621 gtgtttttaa taaacagtcg cagccaccta ttctctgcga ccctccgggg cttacggagc
     1681 aagtccttaa ccttagaggg cataccttct cccgaagtta cggtatcaat ttgccgagtt
     1741 ccttctcctg agttctctca agcgccttag aattctcatc ctgcccacct gtgtcggttt
     1801 gcggtacggt tcgattcaaa ctgaagctta gtggcttttc ctggaagcgt ggtatcggtt
     1861 gcttcgtgtc cgtagacact cgtcgtcact tctcggtgtt aagaagaccc ggatttgcct
     1921 aagtcttcca cctaccggct taaacaagct attccaacag cttgccaacc taaccttctc
     1981 cgtccccaca tcgcatttga atcaagtaca ggaatattaa cctgtttccc atcgactacg
     2041 catttctgcc tcgccttagg ggccgactca ccctacgccg atgaacgttg cgcaggaaac
     2101 cttgggcttt cggcgagcgg gcttttcacc cgctttatcg ctactcatgt caacattcgc
     2161 acttctgata cctccagcac actttacaat gcaccttcat cagcctacag aacgctcccc
     2221 taccatgccg gtaaaccggc atccgcagct tcggttatag atttgagccc cgttacatct
     2281 tccgcgcagg acgactcgac cagtgagcta ttacgctttc tttaaatgat ggctgcttct
     2341 aagccaacat cctggctgtc tgggccttcc cacttcgttt accacttaat ctatcatttg
     2401 ggaccttagc tggcggtctg ggttgtttcc ctcttgacaa cggacgttag cacccgctgt
     2461 ctgtctcccg aggaaccact tgatggtatt cttagtttgc tatgggttgg taagttgcaa
     2521 taacccccta gccataacag tgctttaccc ccatcagtgt cttgctcgag gcactaccta
     2581 aatagttttc ggggagaacc agctatctcc gagtttgttt agcctttcac ccctatccac
     2641 agctcatccc cgcattttgc aacatgcgtg ggttcggtcc tccagtacct gttacggcac
     2701 cttcaacctg gccatggata gatcactcgg tttcgggtct acacccagca actcatcgcc
     2761 ctattaagac tcggtttccc tacgcctccc ctattcggtt aagctcgcta ctgaatgtaa
     2821 gtcgttgacc cattatacaa aaggtacgca gtcacaccac tagggcgctc ccactgtttg
     2881 tatgcatcag gtttcaggtt ctgtttcact cccctcccgg ggttcttttc gcctttccct
     2941 cacggtactg gttcactatc ggtcgatgat gagtatttag ccttggagga tggtcccccc
     3001 atattcagac aggatttcac gtgccccgcc ctacttttcg tacgcttagt accgctgttg
     3061 agatttcgaa tacgggactg tcacccacta tggtcaagct tcccagcttg ttcttctatc
     3121 tcgacagtta ttacgtacag gctcctccgc gttcgctcgc cactacttgc ggaatctcgg
     3181 ttgatttctt ttcctccggg tacttagatg gttcagttct ccgggttcgc ttctctaagt
     3241 ctatgtattc aacttaggat actgcacaga atgcagtggg tttccccatt cggacatcgc
     3301 gggatcattg ctttattgcc agctcccccg cgcttttcgc aggcttacac gtccttcgtc
     3361 gcctatcatc gccaaggcat ccacctgatg cacttattca cttgactcta tcatttcaag
     3421 aacttctttg actttgccta acattccgtt gactagaaca tcagacttga atttcctact
     3481 ttgataaagc ttactgcttt gttgtgtctt aatcctgcct tttgtgtttc aggattaagt
     3541 cgatacaatc atcacccaaa tactgtgttt gttttctttt ctcttgcgag agatttttat
     3601 cctttgcaaa gaataaaaaa tcaaaacaaa cgctttgtct ttgtttgttg atttcggctt
     3661 tccaatttgt taaagatcga tgcgttcgat attgctatct actgtgcaaa tcaaaacgag
     3721 ctgattatta tatcagcatt ttgttcttgg tcaagtgtga cgtcgccctg aatggattct
     3781 gttccattct tccgttttga tttgtacagt attggtggag gcaaacggga tcgaaccgat
     3841 gaccccctgc ttgcaaagca ggtgctctac caactgagct atgcccccgt tcttggtggg
     3901 tctgggagga cttgaacctc cgaccccacg cttatcaagc gtgtgctcta accagctgag
     3961 ctacaaaccc ggattctctt cttaagcgaa tcttgccttc actcaagctt cttccgcatc
     4021 tttttcagtt taccgataag tgtgaatgcc taaagcctct tctttctcta gaaaggaggt
     4081 gatccagccg caggttcccc tacggctacc ttgttacgac ttcaccccag tcatgaagca
     4141 taccgtggta agcggactcc ttgtggttat cctacctact tctggtatcc cccactccca
     4201 tggtgtgacg ggcggtgtgt acaagacccg ggaacgtatt caccgcagta tgctgacctg
     4261 cgattactag cgattccgac ttcatgcact cgagttgcag agtgcaatcc ggactacgat
     4321 cggttttgtg agattggctc cgcctcgcgg cttggctacc ctctgtaccg accattgtat
     4381 gacgtgtgaa gccctggtca taagggccat gaggacttga cgtcatcccc accttcctcc
     4441 ggcttgtcac cggcagtctc attagagtgc ccaactgaat gatggcaact aatgacaagg
     4501 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acagccatgc
     4561 agcacctgtg ttacggctcc cgaaggcact cctccgtctc cggaggattc cgtacatgtc
     4621 aagaccaggt aaggttcttc gcgttgcatc gaattaatcc acatcatcca ccgcttgtgc
     4681 gggtccccgt caattccttt gagttttaat cttgcgaccg tactccccag gcggtcaatt
     4741 tcacgcgtta gctacgctac caagcaatca ggttgcccaa cagctaattg acatcgttta
     4801 gggcgtggac taccagggta tctaatcctg tttgctaccc acgctttcgg gcatgaacgt
     4861 cagtgttgtc ccaggaggct gccttcgcca tcggtattcc tccacatctc tacgcatttc
     4921 actgctacac gtggaattct acctccctct gacacactcg agtcacccag ttcagaacgc
     4981 agttcccggg ttgagcccgg ggatttcaca tcctgcttaa gtaaccgtct gcgcccgctt
     5041 tacgcccagt aattccgatt aacgctcgca ccctacgtat taccgcggct gctggcacgt
     5101 agttagccgg tgcttattct tcaggtaccg tcatcagccg ctgatattag caacagcctt
     5161 ttcttccctg acaaaagtcc tttacaaccc gaaggccttc ttcagacacg cggcatggct
     5221 ggatcaggct tgcgcccatt gtccaaaatt ccccactgct gcctcccgta ggagtctggg
     5281 ccgtgtctca gtcccagtgt ggcggatcat cctctcagac ccgctactga tcgtcgcctt
     5341 ggtaggcctt taccccacca actagctaat cagatatcgg ccgctcgaat agcgcaaggc
     5401 ccgaaggtcc cctgctttct ctctcaagac gtatgcggta ttagctgatc tttcgatcag
     5461 ttatccccca ctactcggta cgttccgata tgttactcac ccgttcgcca ctcgccaccc
     5521 gagaagcaag cttctctgtg ctgccgtccg acttgcatgt gtaaagcatg ccgccagcgt
     5581 tcaatctgag ccaggatcaa actcttatgt tcaatctcta actttttaac ttctggtctg
     5641 cttcaaagaa accaacagga caatgttcaa aacattatct tgtctgtctt tcaaacagtg
     5701 tgagactcaa ggcactcaca cttatcggta atctgttttg ttaaagagcg ttgcgaatta
     5761 taaagtattc cttccgcctg tcaagatatc tctcgatatc cccaacattc tgtgctatac
     5821 ttttcagttc gtccgccact tctgcagcag cgaagaaccg aactatacgc ccaca
//
LOCUS       DAWGRZ010000134         5838 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000134 DAWGRZ010000000
VERSION     DAWGRZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.134"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            16..207
                     /locus_tag="WHT44_001631"
     CDS             16..207
                     /locus_tag="WHT44_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533788.1"
                     /translation="MFQQGTAHQAPHCVLPERDYPPIRFYRHKQTGFNRKGMGIKSIS
                     DISRAMPSENQSPLSDGIV"
     gene            285..1232
                     /locus_tag="WHT44_001632"
     CDS             285..1232
                     /locus_tag="WHT44_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ2533789.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKVVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLTIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1385..2509)
                     /gene="rluD"
                     /locus_tag="WHT44_001633"
     CDS             complement(1385..2509)
                     /gene="rluD"
                     /locus_tag="WHT44_001633"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ2533790.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLTHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPNDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2508..3311
                     /locus_tag="WHT44_001634"
     CDS             2508..3311
                     /locus_tag="WHT44_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ2533791.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHAQQSQLDTAYAYYKDDEKDKALAAIDRFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYKKLDKPRLAADTRRVLETNFPKSPFLKQPWRSDDMPWWRYWH"
     gene            complement(3539..5758)
                     /locus_tag="WHT44_001635"
     CDS             complement(3539..5758)
                     /locus_tag="WHT44_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ2533792.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRVWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPLAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTAVRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVAAAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTASAAQTGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFRAVRNIPA
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLRPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1390 a         1858 c         1407 g         1183 t
ORIGIN      
        1 ttctccgagc tttatatgtt tcaacagggg acggcacatc aagcaccgca ctgcgtgttg
       61 cccgaacgag actaccctcc gattagattc tatcgccata aacagacggg tttcaaccga
      121 aaaggaatgg ggataaagtc catttccgac atctctcggg cgatgccgtc tgaaaaccaa
      181 tctccacttt cagacggcat tgtttaggca cggtcatttt gcaaattaag gttaaggcgt
      241 gtatgcttgc cttcccttaa aaacaaacac gcaacgcatt atttatgaat atcctcagta
      301 aaatcagcag ctttatcgga aaaacatttt ccctctgggc cgcgctcttt gccgccgccg
      361 cttttttcgc gcccgacacc ttcaaatggg cggggcctta tattccttgg ctgttgggca
      421 ttattatgtt cggtatgggt ttgacgctca aaccttccga cttcgatatt ttgttcaaac
      481 atcccaaagt cgtcatcatc ggcgtaatcg cacaattcgc cattatgccg gcaaccgcct
      541 ggctgctgtc caaactgttg aacctgcctg ccgaaatcgc ggtcggcgtg attttggtcg
      601 gctgctgccc gggcggtacg gcttccaatg tgatgaccta tctggcgcgt ggcaatgtgg
      661 ctttgtcggt tgccgttacg tctgtttcca ccctgatttc cccattgctg actcccgcca
      721 tcttcctgat gcttgccggc gaaatgctgg aaatccaagc ggccggtatg ttgatgtcca
      781 tcgtcaaaat ggttttgctc cccattgttt tgggtttgat tgtccataag gttttgggca
      841 gtaaaaccga aaagctgacc gatgcgctgc cgctggtttc cgttgccgcc atcgtgctga
      901 ttatcggcgc ggttgttggg gcaagcaaag gcaagattat ggaaagcggc ctgctgattt
      961 ttgcggttgt cgtactccac aacggcatcg gctacctgct cggcttcttt gccgccaaat
     1021 ggaccggcct gccttatgat gcacaaaaaa cgctgaccat cgaagtcggt atgcaaaact
     1081 cgggcctggc cgccgcgctt gccgccgcac actttgccgc cgcgccggtc gttgccgttc
     1141 cgggcgcatt gttcagcgtg tggcacaata tctccggctc gctgctggca acttattggg
     1201 cggccaaagc cggtaaacat aaaaaaccct aaacgccttt tatcataatg ccgtctgaac
     1261 ggggcaaaca aaaccggcgg tttctgcccg gtcccttcag acggcctttg tttgaaatcg
     1321 gcggggacga ttgcccggct gctccgattc ggctgcctgc gttcgccccg cctttcaagc
     1381 cgcctcactc ccttacataa accacttcga catcatagtc gtcttcgttc caatcatcgt
     1441 cgtcgtccgc gccgaatttg tcctgccatt cttcttcatt gctcaaagac gaatccaaac
     1501 cggcttcaag acggaggacg gataacaaat gatatatgtc gtttggaatc ggtgcttcaa
     1561 acgaaacggt ttcgccgctt tccggatggg tgaaactcaa gcggtaggcg tgcaacgcct
     1621 gacgcgcacc caaactttta acggcttctt tcaccgtgtc gccgcacgga tggcgcgggt
     1681 tgccgtaaac cgggtcggcg gcaagcggat ggttggcctc gcgcatatgg acgcggattt
     1741 ggtgcgtcct gcccgtttcg agcgagcatt cgatatagct gtgggtaaga tagcgttcca
     1801 acactttgac gtgggtaacg gctggtttgc cgccgaattt gacgactgcc attttcaggc
     1861 ggttgtgcgg atcgcgtccg atttgggttt cgattttacc gtcgaagggg acgatgccgt
     1921 tggcgacggc gcggtagatg cgtttgaccg tgcgctcttg aagctgcctc acgagggaat
     1981 tttgcgccgg cagggttttg gcaaccacca tcagcccgct ggtttccttg tccaaacggt
     2041 gtacgatgcc cgcgcgcggt acttggctca attcgggaca gtgcgccaac aggccgttga
     2101 gcagcgtccc cgtccagttg cccgccgccg gatgcaccac cagtccggcc ggtttgttga
     2161 cgacgatgac ggtatcgtct tcgtaaacaa tatccaaagc cataggctct ggaacaaacg
     2221 ccagattttc ctcactcgga cggacggtta cacaaatttg ctcgccgcct atcattttgt
     2281 ctttgggttg cgaaggttta tcgtttacaa taaccgcgcc ttctttaatc catgatgtca
     2341 ggcggctgcg cgagtagtcg ggcagaagtt tcgccaatac cgcatccaac cgcccgcctg
     2401 caagctcgag cggaacggtc agaccgatac ggttttcagt ttcggaagct gacgcaaagt
     2461 ctgaatcgtc gctataatcg gattcattat caaaggaagt attctgcatg aaaaaaattc
     2521 ttttaacggt ttcattaggt ttggcactga gtgcctgtgc cactcaaggt acggtagata
     2581 aagatgccca gattacccaa gactggagtg tggaaaaact ctatgccgaa gcccaggacg
     2641 agctgaacag cagcaattat acgcgagctg tcaaattata cgaaatcttg gaatcgcgct
     2701 tccctaccag ccgccatgcc cagcaatccc aactggatac ggcatacgcc tactataaag
     2761 atgatgagaa ggataaggcg ctggccgcca ttgaccgctt ccgccgcctt caccctcaac
     2821 acccgaatat ggactacgcg ctatacctgc gcggtttggt actgttcaac gaagatcaat
     2881 ctttcttgaa caagctggcc tcgcaagact ggtccgaccg cgacccgaaa gccaaccgcg
     2941 aagcgtatca ggcctttgcg gaactcgtcc aacgcttccc gaacagcaaa tacgccgccg
     3001 atgcgaccgc acgcatggtc aaactggtcg atgctttggg cggcaatgaa atgtcggtag
     3061 cgcgttacta catgaaacgc ggcgcatata tcgccgccgc caaccgcgcc caaaaaatta
     3121 tcggcagcta tcaaaataca cgctatgtcg aagaatcgct cgccatattg gaacttgcct
     3181 acaagaagtt ggacaagccc cggcttgccg ccgatacgcg ccgcgtgttg gaaaccaact
     3241 tcccgaaaag cccgttctta aagcagccgt ggcgatctga cgatatgccc tggtggcgtt
     3301 actggcactg attccgcacc gatgtccgga tgccgtctga agccctttca gacggcattt
     3361 ccccttctgg cgcaaacctt tccgttcatt caaacggttc tccctgccgt taaccgcaac
     3421 gccctttctg tttttgacgc acactgaaaa tcaaacgata tgccttgcca gcacgaaccg
     3481 gcgttaacgc caaaatgccg tctgaatccg tttcagacgg cattttatgt ttcaaacctc
     3541 actcaaacgg ttttttctgc caatagaatt tataccctct caaacgttga gccttcacgc
     3601 cgccgcgtcc caagccgaat tgcagcgcac ccgacaaatc ggtacgcagc agtttaatgc
     3661 cgtgtgcgcg gacacggttc tgcaccgctt cggtcggatg tttgtaggcg ttggcataac
     3721 cgcttgaagc aacggcatat tcgggcgaaa cggcattgag gaacacgccc gacgaggacg
     3781 tattgctgcc gtgatgcccc aacaccaaca cctggctgta caggttgcct ccatacttgc
     3841 cgaccaggct ttcctcgccc ttcgtatcca aatcgcccgt taccagcagt gccgcaccgc
     3901 ccgccacaac acgcaaaaca caacttttcc cattatcatc gatgtttttg cgttcagacg
     3961 gcctcaaaaa ctcgaaatct acgccgtccc attgccaacg ctgttccgca caatgccgcg
     4021 ccccctcata aaattccggc tgcccggcat aaatcccgcc ggcgggaata ttcctcaccg
     4081 cccgaaaacc gccgtcgtgg tcgctgtcgt gatgcgacag aaccagcttg tccaaacggc
     4141 ggacacccgc cgcattcaaa ctcggcacaa tccccgtctg tgccgccgat gcagttccag
     4201 tgtcaaacaa aagatgatga tttgccgtct gaaccgacac cgacaaaccc tgccccgcat
     4261 cccaaaccgt aaccgcaacc tcattttccg gcacgccggg tgaacggtaa aacacaaacc
     4321 ctgccaacag caacaccgcc cacggacgca aacccaagcc gcgcggcagc agcaacagca
     4381 aagcggcaca caccgccaac accaacagcg gcaaaggtgc ggcggcaacg gcaaactcgg
     4441 gcgacacatc ggcaagccac accaaaaacc gcaaagtata ttccgccaaa aatgccccca
     4501 actgttgcaa aggcgcaaac ggcacgaccg aacccagcaa cgccagcggc gtcaataccc
     4561 aagaaaacca cggaatcgcc accgcattga ccaaagggct gattaaaggc agcgaagcaa
     4621 acagataacc gagcaaaacc agcgacaaca ccgaagccgc ccactgcccg cgcacggcgg
     4681 tttgccgttt cccttcgtgc aaacgccccg aacacgccca tatcagggcc gccaccaaac
     4741 cgaaagacag ccaagtcccc acacccaaga ccgccaaagg gtcgaacagc agcactgccg
     4801 ccaacgcctg ccaccacgtc gcccacgccg acaatcttcc cctgcgccaa gcccacgcaa
     4861 acgccgccaa catcaaaacg ctgcgctgcg tcggcacgga aaaaccggca agcagcgcgt
     4921 aaaacagcgc gcctgcacac cccgccgcca aaacccacac gcgcggccgg gcaggaaggc
     4981 gcggggaaca ggcaagcagc cgcttcgcca gccacgcaaa catcaccgcc accatcgtaa
     5041 cgtgcaaacc cgaaatactg accaaatgcg tcagccccaa cggtcggaac gcctgccaca
     5101 attcggggcg caatgccgac tgttcgccca cgctcaacgc gcgcatcagc ccgatgccgt
     5161 ctgaaagccc gccgtccgca tccgcctgct gccaattacg gctgatgcgg ctgcgccaaa
     5221 ccgcaatccc ccaaccgctt ccgccatgca gcaaaaccct gtccgcaccg accgtccccg
     5281 cgccgcctat cccgttggat aatgcccacg cctcacggtt caaaccgcgc aggttcaatt
     5341 cgccgacgac ggggtgcaca cgtgccgtta tccgccatct gctcccgacc gcccattcgc
     5401 gccgtttgta gtccgacagc agcaaatcaa acgtccgacc accgctgtcc acagcctttg
     5461 ccgcaaactg cacgcgccgc ccgtccgacc tcggcatatc cgccacttcc accgtcaacg
     5521 gcacacctga aaccgcctcc gcccgccatt gcgaagacag tgccgcttcc gttctgaata
     5581 cgccgtaagc cgcgcccgcc aacacgcaca gcatcagacc ggcaaacgca aaacgccttg
     5641 caaacacagc aaaaaccgca aaagccgcca gccaaaacag ccaatgcggc acgaccggca
     5701 gcgcaaacga agccgccgcg ccgacaaccc aacacggcaa aaaccaacga cggaacacgt
     5761 ctccccaccc cagatatttc aaaaataaac ggataataaa gcaaatccga aaaaccgtca
     5821 taacaagatt tgaaaatg
//
LOCUS       DAWGRZ010000135         5708 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000135 DAWGRZ010000000
VERSION     DAWGRZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.135"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            227..5650
                     /gene="iga"
                     /locus_tag="WHT44_001636"
     CDS             227..5650
                     /gene="iga"
                     /locus_tag="WHT44_001636"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ2533793.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADEIKQRDNAGTIKGNGEHHWKTTGT
                     NSHIGSTAVRLANNERDANNGQNVTFENNGTLVLDQNINQGAGGLFFKGDYTVKGINN
                     DITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVIL
                     NQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFEH
                     IRNVDEGARIVNHNTGHASTITLTGKSLITDPKTISIHYIQNNDDDDAGYYYYRPRKP
                     IPQGKDLYFKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQEEAKKNAMNHKNN
                     QRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLSG
                     RPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKATEIAVNQSASFSSGRNVS
                     DITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALNSFGATQINGNV
                     NLNQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNNA
                     SDAQSANKYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGHYQLHVQDKTGEP
                     NQEGLNLFDASSVQDRSRLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRVK
                     PAPSPATNTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKRQ
                     QAEAERKSAELAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREAAELSAKQK
                     VEAEREAQALAVRRKAEAEEAKRQAAELARRHEKEREAAELSAKQRVGEEERRQTAQS
                     QPQRRKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELARRQQEERKAAELL
                     AKQRAEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELSANQKAAAEAQAL
                     AARQQKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAAKRQQEARQTAEL
                     ARRQEAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRKRRAAPQDY
                     MAASQNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDALEDEN
                     VSESVDTSDKQPQDNTELHEKYENDYEEIPLDALEDEDVSESVDTSDKQPQDNTELHE
                     KVETVSLQPRAAQPRAQAAAQADAVSTNTNSALSDAMASTQSILLDTGASLTRHIAQK
                     SRADAEKNSVWMSNTGYGRDYASAQYRRFSSKRTQTQIGIDRSLSENMQIGGVLTYSD
                     SQHTFDQASGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRSRLQTQQKANFNRASIQ
                     TGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKVSSMSVKTLTAGLDFAYR
                     FKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFVYKADNQQQYSAGAALLYRNVTLNVN
                     GSITKGKQLEKQKSGQIKIQIRF"
BASE COUNT         1925 a         1360 c         1279 g         1144 t
ORIGIN      
        1 aaacaaaaag ccgcagaaat ttaacggaag aaccgaaccc cgaaaatcgg cttcccgcct
       61 tcgcttgaat aacagaacaa aggaagcgga aataaaaaca tcgggattct ttttttaaat
      121 tggcaataaa acaccaaaat gaatgattta cacaacaaaa aatagtcaac accaccaaac
      181 cggcgtaaaa tacaaaacat tatcaccctt aaaacggtaa aaccttatga aaaccaaacg
      241 ttttaaaatt aacgccatat ccttatccat ctttcttgcc tatgccctta cgccatactc
      301 agaagcggca ttggtcagag acgatgtcga ttatcaaata ttccgtgact ttgcagaaaa
      361 caaaggcaaa ttttttgtcg gcgcaaccga tttatcagtg aaaaacaaac aaggtcaaaa
      421 catcggcaac gcattatcca acgtaccgat gattgatttt agcgttgcag atgtcaacag
      481 acgtacatta accgtcatcg atccccagta tgccgtcagc gtcaaacatg taaaaggaga
      541 cgaaatctct tattacgggc atcacaatgg gcacttagat gttagcaatg atgaaaacga
      601 atatcgttca gttgcacaaa atgactacga accaaataaa aattggcatc acggtaatca
      661 aggtcgtctt gaagactaca acatggcacg ccttaataaa ttcgtaacag aagtcgcacc
      721 tattgcacca accagtgcag gcggcggcgt tgaaacctat aaagataaaa accgtttttc
      781 tgagtttgtg cgagttggag caggtacgca atttgaatat aacagccgct acaatatgac
      841 agagctttca cgagcttatc gttatgccat tgcaggtacg ccttatcaag acgtgaatgt
      901 cacatcgaat ctaaatcaag aaggcttgat tggcttcggt gataattcaa aacatcattc
      961 cccagaaaaa ctcaaagaag ttttatctca aaacgcattg actaactatg ctgtgttagg
     1021 cgatagcggt tcaccattat ttgcttatga taaacaagaa aaacgctggg tctttttagg
     1081 tgcttacgat tattgggcag gctatcaaaa aaactcttgg caagaatgga atatctataa
     1141 aaaagaattt gcagatgaaa tcaaacaacg cgataacgcc ggcaccatca aaggtaatgg
     1201 agaacatcat tggaaaacca cgggcacaaa cagccatatc ggttcgacag cggtaaggct
     1261 tgccaacaat gaaagggatg caaacaacgg acagaatgtt acctttgaaa acaacggcac
     1321 tttggtattg gatcaaaaca tcaaccaagg cgcgggcggt ctgtttttca aaggcgatta
     1381 cacagtcaaa ggtataaata atgacatcac ttggctaggt gcggggattg atgttgccga
     1441 cggcaaaaaa gtcgtttggc aagtcaaaaa tccgaatggc gacagattgg caaaaatcgg
     1501 caaaggcaca ttagaaataa acggcacagg cgttaaccaa gggcaattaa aagtcggcga
     1561 cggtacggtt attctgaatc aacaagccga tgccgacaaa aaagtccagg ctttctccca
     1621 agtcggcatt gtcagcggac gcggtacatt ggtattaaat agttcaaatc agattaatcc
     1681 cgataaccta tatttcggtt tccgtggcgg tcgtttggat gccaatggca atgacttgac
     1741 ttttgaacac atccgcaacg tggatgaagg cgcgcgcatt gtcaaccaca acacaggcca
     1801 cgcctccaca atcacgctaa cgggtaaatc tttgattacc gatcccaaaa ccatctctat
     1861 tcattatatt caaaataatg atgatgacga tgctggttat tactattacc gccctaggaa
     1921 accgattcca caaggcaaag atctttattt caaaaactac cgttattacg ccctaaaatc
     1981 cggcggcagc gtgaacgcac cgatgcccga aaacggacaa acggaaaata acgactggat
     2041 tttaatgggc agcacacaag aagaagccaa gaaaaacgcg atgaaccaca aaaacaatca
     2101 gcgtattagc ggttttagcg gtttctttgg cgaagaaaac ggaaaaggac ataacggtgc
     2161 attaaacctt aatttcaacg gcaaaagcgc gcaaaaccgt ttcttgttaa caggcggcac
     2221 taatttaaac ggaaaaataa gcgtaactca aggcaatgtc ttgttatcag gtcgtccaac
     2281 accacacgca agagattttg tgaacaaatc ttcagcccga aaagacgcac atttctccaa
     2341 aaacaatgaa gtcgtatttg aagacgactg gataaaccgc acattcaaag ccacagaaat
     2401 tgcggttaat caatccgcat cattctcttc cggcagaaat gtatccgaca tcaccgccaa
     2461 catcaccgca acagacaacg ccaaagtaaa tttgggttac aaaaacggcg atgaggtttg
     2521 cgtgcgctcg gactataccg gttacgttac ctgcaacaca ggcaacttat ccgataaggc
     2581 tttaaacagc tttggagcga cacagattaa cggcaatgtg aatctgaacc agaatgcggc
     2641 attggtcttg ggcaaggctg cattatgggg gcaaattcaa gggcaaggaa acagccgtgt
     2701 cagcctaaac caacatagca aatggcattt gaccggcgac agccaagtac acaatctgtc
     2761 attggcggat agccatattc atttgaacaa tgcctccgat gcgcaaagtg caaataaata
     2821 ccacacgctc aaaatcaatc atttatccgg taacgggcat tttcattatc tgacgcacct
     2881 ggcgaaaaat cttggggata aagtgcttgt gaaggaatcc gcatccggtc attatcagct
     2941 ccatgttcaa gataaaacag gcgaacccaa tcaggaaggg ctgaatctct ttgatgcatc
     3001 atccgtacaa gaccgctccc gcctttctgt ttccttggcg aataaccatg tagatttagg
     3061 ggcattgcgt tatacaatca aaacagaaaa cggtattacc cgattgtaca atccttatgc
     3121 cgagaaccgc cgccgagtca aaccggcccc gtctcctgcc acaaacacgg cttctcaagc
     3181 acaaaaggca acacaaacgg acggtgcaca aattgccaag cctcaaaata tcgtcgtcgc
     3241 accgcctagc ccgcaggcaa atcaagccga agaagcaaaa cgccagcaag caaaagcgga
     3301 gcaagtgaag cgtcagcaag cagaagcaga gcgtaaatcg gcagagttag caaaacaaaa
     3361 agcagaagcc gaacgcgaag caagagagct ggcgacccgc caaaaagcag aacaagaacg
     3421 cagcagcgcc gaacttgcac gtcgacatga aaaagaacgc gaagcggcag agttgtcggc
     3481 aaaacaaaaa gtcgaagccg aacgtgaggc tcaagcattg gcggtacgcc gaaaagcaga
     3541 agccgaagag gcaaaacgcc aagctgcaga gcttgcacgc cggcatgaaa aagaacgcga
     3601 agcggcagag ttgtcggcaa aacaaagggt cggggaagag gaacgccgtc aaacagccca
     3661 atcccagccg caacgccgca aacgccgtgc cgcaccgcag gattatatgg cagcttccca
     3721 agaccgtcct aaacgccgcg gacaccgttc ggtacagcaa aacaatgtgg aaattgccca
     3781 agcccaagca gaacttgcgc gccgacagca ggaagaacgc aaagcggcag agttgttggc
     3841 aaaacaaagg gcagaagccg aacgtgaagc tcaagcattg gctgcgcgcc gaaaagcaga
     3901 agccgaagag gcaaaacgcc aagctgccga acttgcacat cgccaggaag cagaacgcaa
     3961 agcggcagaa ttgtcggcaa accaaaaagc cgcagctgaa gcccaagcat tggcggcacg
     4021 ccaacaaaaa gcccttgcgc gtcaacagga agaagcacga aaagctgcag agttagccgt
     4081 caaacaaaaa gcggaaaccg aacgcaaaac cgccgaactt gccaagcaaa gggcagcagc
     4141 cgaagcagca aaacgccagc aagaagctcg ccagaccgcc gaacttgcac gtcgtcaaga
     4201 agcggaacgc caagcagcag agttgtcggc aaaacaaaaa gccgaaacag atcgagaagc
     4261 ggcagaatcg gcgaaacgaa aagctgagga agaagagcac cgtcaagcag cccaatccca
     4321 gccgcaacgc cgaaaacgcc gtgccgcacc tcaggattat atggcagctt cccaaaatcg
     4381 tccgaaacgt cgcggacgca gatctactct gccggcaccg ccctcgccat catttgattc
     4441 gtcagcttac gcagcaccca gggccttgca taatccggac tggtatgaaa atgactatga
     4501 agaaatcccc ttggatgcgt tggaagatga aaatgtatct gaatcagttg acacatcaga
     4561 caaacagcct caagacaata cggaacttca tgaaaaatat gaaaatgatt atgaagaaat
     4621 ccccttggat gcgttggaag atgaagatgt atccgaatcg gttgacacat cagacaaaca
     4681 gcctcaagac aatacggaac ttcatgaaaa agttgagaca gtgagtttgc aaccaagagc
     4741 cgcgcagccg cgagcccaag ccgccgcgca agccgatgca gtcagcacca ataccaactc
     4801 ggctttatct gacgcaatgg caagcacgca atctatcttg ttggatacag gtgcttcatt
     4861 aacacggcac attgcacaaa aatcacgcgc tgatgccgaa aaaaacagtg tttggatgtc
     4921 gaacaccggt tatggtcgtg attatgcttc cgcacaatat cgccggttta gttcgaaacg
     4981 cacgcaaaca caaatcggca ttgaccgcag cttgtccgaa aatatgcaga taggcggcgt
     5041 attgacttac tctgacagtc agcatacttt tgatcaggcg agcggcaaaa atacttttgt
     5101 gcaagccaac ctttatggta agtattattt aaatgatgct tggtatgtgg ccggcgatat
     5161 tggtgcaggc agcttgagaa gccggttaca aacgcagcaa aaagcaaact ttaaccgagc
     5221 aagcatccaa accggcctta ctttgggcaa tacgctgaaa atcaatcaat tcgagattgt
     5281 ccccagtgcg gggatccgtt acagccgcct gtcatctgca gattacaagt tgggtaacga
     5341 cagtgttaaa gtaagttcta tgtcagtgaa aacactaacg gccggactgg attttgctta
     5401 tcggtttaaa gtcggcaacc ttaccgtaaa acccttgtta tctgcagctt actttgccaa
     5461 ttatggcaaa ggcggcgtga atgtgggcgg taattccttc gtctataaag cagataatca
     5521 acagcaatat tcagcaggtg ccgcgttact gtaccgtaat gttacattaa acgtaaatgg
     5581 cagtattaca aaaggaaaac aattggaaaa acaaaaatcc ggacaaatta aaatacagat
     5641 tcgtttctaa aatactaaat tcatagcaaa ataaaatgcc gtctgaactc aggcttcaga
     5701 cggcattt
//
LOCUS       DAWGRZ010000136         5708 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000136 DAWGRZ010000000
VERSION     DAWGRZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.136"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            72..998
                     /gene="fabD"
                     /locus_tag="WHT44_001637"
     CDS             72..998
                     /gene="fabD"
                     /locus_tag="WHT44_001637"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ2533794.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFAEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LNFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKAICAEAAQSEVVEAVNFN
                     SPGQIVIAGNAAAVGRAMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQVAAFIEAH"
     gene            1136..3001
                     /gene="msbA"
                     /locus_tag="WHT44_001638"
     CDS             1136..3001
                     /gene="msbA"
                     /locus_tag="WHT44_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ2533795.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLFALQTANLQSLIDSSPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            3101..4666
                     /gene="guaA"
                     /locus_tag="WHT44_001639"
     CDS             3101..4666
                     /gene="guaA"
                     /locus_tag="WHT44_001639"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ2533796.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDGEPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNESKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVIALRAVITSDFMTAHWAELPYSLLGKVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            4728..5474
                     /gene="fabG"
                     /locus_tag="WHT44_001640"
     CDS             4728..5474
                     /gene="fabG"
                     /locus_tag="WHT44_001640"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ2533797.1"
                     /translation="MSTQDLNGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETIESLIADIEKAFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRSGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         1387 a         1819 c         1376 g         1126 t
ORIGIN      
        1 ttttgtcccg cctgaatttt tcgtaaaact cgggcagaat acctgattat ccaaccaaac
       61 aaaggaatac tatgtctttt gccttctttt ttcccggaca aggttcccaa agcctcggta
      121 tgatgaacgg ctttgccgaa cacgccatcg tcaaaaacac ctttgccgaa gcctccgcca
      181 tattggggca ggacttgtgg gcgatgataa acggcagcga tgccgaaatc atcggtcaaa
      241 ccgtcaacac ccagcccatt atgctcgccg ccggcgttgc cgtttaccgc gcctatttag
      301 aagcgggcgg caaaacgcct gccgccgttg ccggacacag cctcggcgaa tacaccgcac
      361 tcgttgccgc cggcgcattg aattttgccg acgcggtcaa actcgtgcgc ctgcgcgccg
      421 aactgatgca gtccgccgta ccgcaaggcg tgggcgcaat ggcggcgatt ctcggcttgg
      481 aagatgagca ggttaaagcc atttgtgccg aagccgccca aagcgaagtg gtcgaagccg
      541 tcaacttcaa ctcacccgga caaatcgtga ttgcaggcaa cgccgccgcc gtcggacgcg
      601 ccatggctgc cgccaaagaa gccggtgcca aacgcgccct gccgctgccc gtgtccgtac
      661 cttcccattg cagcctgatg aaacccgccg ccgacaaact tgccgaagcc ctgaaaaccg
      721 ttgaaatcaa gcagccgcaa atccgcgtta tccacaacgc cgacgttgcc gcctacgatg
      781 atgccgacaa aatcaaagac gcgctcgtcc gccagcttta cagccccgta cgctggacgg
      841 aaaccgtcaa cgccctcgtt tcagacggca ttgccgaatc cgccgaatgc ggcccgggca
      901 aagtgttggc gggcttggca aaacgcatca acaaagccgc cgcgtgcagc gcactgaccg
      961 atgccggaca ggttgccgcc tttatcgaag cgcactgact tcgttctgca aaaagcagcc
     1021 tgccctcttc aggctgcttt tcatgtccga acgacggcag ccccatattt acgctataat
     1081 ccatcccgac caaaccaccg acagcggctg ccgttgcagt tcccgcccta ccgatatgat
     1141 agaaaaactg actttcggac tgtttaaaaa agaagacgcg cgcagcttta tgcgcctgat
     1201 ggcgtacgtc cgcccctaca aaatccgcat cgttgccgcc ctgattgcca ttttcggcgt
     1261 tgccgccacc gaaagctacc ttgccgcctt catcgccccc ctgattaacc acggcttttc
     1321 cgcacctgcc gcgccgcccg agctgtctgc cgccgccggc atcatttcca ccctgcaaaa
     1381 ctggcgcgaa cagtttacct atatggtttg ggggacggaa aacaaaatct ggaccgtccc
     1441 gctcttcctc atcatcctcg tcgtcatccg tggcatctgc cgctttacca gcacctatct
     1501 gatgacttgg gtctccgtga tgaccatcag caaaatccgc aaagatatgt ttgccaaaat
     1561 gctgaccctt tcctcccgct accatcagga aacgccgtcc ggcaccgtac tgatgaatat
     1621 gctcaacctg accgaacagt cggtcagcaa cgccagcgac atcttcaccg tcctcacgcg
     1681 cgacacgatg atcgttaccg gcctgaccat cgtcctgctt tacctcaact ggcagctcag
     1741 cctcatcgtc gtcctgatgt tccccctgct ctccctgctc tcgcgctact accgcgaccg
     1801 tctgaaacac gtcatttccg actcgcaaaa aagcataggc acgatgaaca acgtgattgc
     1861 cgaaacccat cagggacacc gcgtcgtcaa gctgttcaac gggcaggcgc aggcggcaaa
     1921 ccggttcgac gcggtcaacc gcaccatcgt ccgcctcagc aaaaaaatca cgcaggcaac
     1981 ggcggcacat tccccgttca gcgaactgat cgcctcgatc gccctcgccg tcgtcatctt
     2041 catcgccctg tggcaaagcc aaaacggcta caccaccatc ggcgaattta tggcattcat
     2101 cgtcgcgatg ctgcaaatgt acgcccccat caaaagcctt gccaacatca gcatccctat
     2161 gcagacgatg ttcctcgccg ccgacggtgt atgtgcattt ctcgacaccc cgcccgaaca
     2221 ggacaagggc acgctcgcac cgcagcgtgt cgaagggcgc atcagcttcc gcaacgtcga
     2281 tgtcgaatac cgttcagacg gcatcaaagc cctcgacaac ttcaacctcg acatcagaca
     2341 aggcgaacgc gtcgccctgg tcggacgttc cggcagcggc aaatccaccg tcgtcaacct
     2401 gctgccccgc tttgtcgaac cgtctgccgg caacatctgc atagacggta tcgacatcgc
     2461 cgacatcaaa ctcgactgcc tgcgcgccca attcgccctc gtctcccaag acgtattcct
     2521 gtttgacgac accctgtttg aaaacgtccg atacagccgt cccgacgcgg gcgaagccga
     2581 agtcctgttc gccctccaaa ccgccaacct gcaaagcctg attgacagct ccccgctcgg
     2641 actgcaccag cccatcggat cgaacggcag caacttatcc ggcggacagc ggcaacgcgt
     2701 cgccattgcc cgcgccattt tgaaagacgc gccgatatta ttattggacg aagccaccag
     2761 cgcattagac aacgaatccg aacgcctcgt ccaacaggcg ctcgaacgcc tgatggaaaa
     2821 ccgcaccggc atcatcgtcg cccaccgcct gaccaccatc gaaggggccg accgcatcat
     2881 cgtgatggac gacggcaaaa tcatcgaaca aggcacacac gaacaactga tgtcccaaaa
     2941 cggttactac acgatgttac gcaatatctc aaacaaagat gccgccgtcc ggacggcata
     3001 aacaaaatgc cgtccgaaat ggtacaatcg ccccgaccct ttcagacggc atcatatccg
     3061 ccgacccatc cgattatctt caatcactgt aaaacccatt atgacccaag acaaaatcct
     3121 catccttgac ttcggttcgc aagttaccca gctcatcgcc cgccgcgtgc gcgaagccca
     3181 cgtttactgc gagctgcatt ctttcgatat gcctttggac gaaatcaaag ccttcaaccc
     3241 caaaggcatc atcctctccg gcggccccaa ttccgtttac gaatccgact atcaagccga
     3301 taccggtatt tttgatttgg gcattccggt tttgggcatc tgttacggca tgcagtttat
     3361 ggcgcaccac ttgggcggcg aagtgcagcc cggcaaccag cgcgaattcg gttatgcgca
     3421 agttaaaacc atagacagcg agctgacacg cggcattcaa gatggtgagc caaacacact
     3481 cgacgtatgg atgagccacg gcgacaaagt gtccaaactg cccgacggtt tcgccgtcat
     3541 cggcaacacc ccgtcctgcc cgattgccat gatggaaaac gccgaaaaac aattctacgg
     3601 catccagttc caccccgaag ttacccacac caaacaaggc cgcgccctgt tgaaccgctt
     3661 tgtcttggat atttgcggcg cacaaccggg ctggacgatg ccgaactaca tcgaagaagc
     3721 cgttgccaaa atccgcgaac aggtcggcag cgacgaagtg attttaggtc tgtccggcgg
     3781 cgtggactct tccgtagccg ccgcgctgat tcaccgcgcc atcggcgacc aactgacctg
     3841 cgtgttcgtc gatcacggtt tgttgcgcct gaacgaaagc aaaatggtga tggatatgtt
     3901 cgcccgcaac ttgggtgtga aagtgataca cgtcgatgcc gaagggcagt ttatggcgaa
     3961 actcgccggc gtaaccgacc ccgagaaaaa acgcaaaatc atcggtgcgg aatttatcga
     4021 agtatttgat gccgaagaaa aaaaacttac caacgccaaa tggttggcac aaggcacgat
     4081 ttaccctgac gtaatcgaat ccgcaggtgc aaaaaccaaa aaagcccacg ccatcaaatc
     4141 gcaccacaac gtcggcggcc tgcctgaaaa catgaagctc aaattgcttg agcctttgcg
     4201 cgatttgttc aaagacgaag tacgcgaatt gggtgtggct ttgggcctgc cgcgcgaaat
     4261 ggtgtaccgt catccgttcc cgggtccggg tttgggcgtg cgtattttgg gcgaagtgaa
     4321 aaaagaatat gccgacctgc ttcgtcaggc agacgatatt ttcattcaag aattgcgcaa
     4381 tactaccgat gaaaacggta catcttggta cgacctgacc agccaggcat tcgccgtgtt
     4441 cctgcccgtc aaatctgtcg gcgtaatggg cgacggccgc acatacgatt acgtcattgc
     4501 cttgcgtgcc gtgattacca gcgactttat gaccgcgcat tgggcggaac tgccgtattc
     4561 cttgttgggc aaagtgtcca accgcatcat caacgaagtc aaaggcatca accgcgtggt
     4621 ttatgatgtg agcggcaaac cgcctgccac catcgagtgg gaataaacag caaacatggc
     4681 tgccccgtcc ggcgcagtcc ttcgattatc ggaaaaaagg aaaaaatatg agcacacaag
     4741 atttaaacgg caaaatcgct ttggtaacag gcgcatcgcg cggtatcggt gcagcaattg
     4801 ccgacacgct ggcggcagcc ggtgccaaag tcatcggtac ggcgaccagt gagagcggtg
     4861 cggcggcgat tagcgagcgg ttggcgcaat ggggcggcga aggccgcgta ttaaattccg
     4921 ccgaacctga aaccatcgaa agcctgattg ccgacatcga aaaagcgttc ggcaaactcg
     4981 atattctggt caacaacgcc ggcatcaccc gcgacaacct cctgatgcgc atgaaagaag
     5041 aagagtggga cgacatcatg caggtcaacc tcaaatccgt gttccgcgct tctaaagccg
     5101 ttttgcgcgg tatgatgaaa caacgttccg gccgcatcat caacatcaca tccgtcgtcg
     5161 gcgtgatggg caatgccggt caaaccaact atgccgcggc aaaagcaggc ttaatcggtt
     5221 tctccaaatc catggcgcgc gaagtcggca gccggggcat taccgtcaac tgcgtcgccc
     5281 ctggctttat cgataccgac atgacacgcg ccctgccgga agaaacccgc caaaccttta
     5341 ccgcccaaac cgccttgggc agattcggcg acgcgcaaga catcgccgat gcggttctgt
     5401 tcctcgcttc cgaccaagca aaatacatca ccggccaaac gctgcacgtc aacggcggta
     5461 tgctgatgcc ttaacagaca actttttcaa ccatgccgtc tgaagccctt tcagacggca
     5521 tttgcattct caggcaaaat gaacacacac cacaccccgc cctgcccatg cggctcaggc
     5581 acaagctgag acctttgcaa aattcctttc cctcccgaca gccgaaaccc caacacaggt
     5641 tttcagctgt tttcagctgt tttcgcccca aataccgcct aattctaccc aaataccccc
     5701 ttaatcct
//
LOCUS       DAWGRZ010000137         5693 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000137 DAWGRZ010000000
VERSION     DAWGRZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.137"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            98..814
                     /locus_tag="WHT44_001641"
     CDS             98..814
                     /locus_tag="WHT44_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ2533798.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            963..1865
                     /locus_tag="WHT44_001642"
     CDS             963..1865
                     /locus_tag="WHT44_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ2533799.1"
                     /translation="MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFAFGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASTDGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            1908..2681
                     /locus_tag="WHT44_001643"
     CDS             1908..2681
                     /locus_tag="WHT44_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ2533800.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEVGSLKRHHGGASSGGNLPEGLPADRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVVSELVRRRGSLRVITNNIHAASIASARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2793..4481
                     /locus_tag="WHT44_001644"
     CDS             2793..4481
                     /locus_tag="WHT44_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ2533801.1"
                     /translation="MLNKDQFADNHFIRTIIEEDLESGKHTAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLIKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSVEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDCVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4574..5530
                     /gene="gshB"
                     /locus_tag="WHT44_001645"
     CDS             4574..5530
                     /gene="gshB"
                     /locus_tag="WHT44_001645"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ2533802.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     NGGLVTAQASAFEFLGAKNDDDHAWFKSADKVQTALEAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQAMRDFNEKLAILNFSRFIAPTLVTTRSADVRTFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMQLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELGGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
BASE COUNT         1326 a         1540 c         1579 g         1248 t
ORIGIN      
        1 aatttgtgca atgctttaat tcctctgaca cgatttgaaa cacagccgcc ttaaaacatc
       61 ttaaaaaatt tgaaagcgta aaaatacgga aaacatcatg aactttgaaa acgacgacat
      121 tatccatgcg ccgaccacgt cttccctgat tctcgaagag cggcacgatt cggagctgtt
      181 ccgtgtttac gccctgattt tggacggcat taccgatcag gtgctgctgc ccggcaaaaa
      241 gctgaccgaa tccgaacttt gccgtcagat ggtgtgttcg cgcaacaccg tccgcggcgc
      301 gctgtcgctt ttggcgcacg acaagattgt cgatttgcaa cccaacaggg gcgcgttcgt
      361 ccacgttccc gatttgaaag aaatgcagga tgtgttcaat gcgcgcatcg aaatggagac
      421 gatgattttg aatatcctcg cagatttgcc ggatttggaa acgcgcctca agccgcttta
      481 tgcgatgata cggcgcgaag aagaggcctc cggcaggggc gaccgcgtcg gctggaaccg
      541 cctgtccaat gccttccacg tcgaactggc gcgcctggtg ggcaacgatg tgctgttcga
      601 cattatgaat acgctgtgcg cgcggtcttc cctgattgtc gccgtggcgg gcgtgcatcg
      661 cgaggaaaaa cacgccatca atacgcatac gcattccgaa caccgcgaaa tcctcgacct
      721 gctgctggcg ggcaggcgca accgcgtggt caaaatcctg cgccgccatt tgggcaactg
      781 tatggagcgt ttggaaaaga ctttggaaga ttgaatgccg gagcggataa gccgcctgaa
      841 ccttcaggcg gttttttaat ggcgaacctg atgccgtctg aaatatggat gcgggtatct
      901 gcaattttca gacggcaatt tttaaaccgc acatatcatg cggcaataaa ggagagcagt
      961 agatggacag cctgatgacg ttgctttcgg tattgatacc gatgtttgcc ggatttttta
     1021 tccgtgtgcc caagccttac ctgcccgctt tggataaggt gctatcggtc ttggtgtatg
     1081 ctgtgctgct gctgatcggc gtctcgttgt cgcgcgtgga ggatttgggt tcgcggttgg
     1141 acgatatggc gttgacggtt ctgtggctgt ttgtttgtac ggtcggggcg aacctgcttg
     1201 ctttggcagt gttgggaaag ttattcccgt ggcggataaa ggggaaaggg aagggcgttt
     1261 cggtcggcgt gtcgggcagt gtggggcagc tcggatgcgt gctgctcgga tttgcattcg
     1321 gcaaactgat gcgcgatatt tggatgccgt ctgaaagcgc gggcatgtat tgtctgatgc
     1381 tgctggtgtt cctcatcggc gtacagctca aaagcagcgg cgtatcgttg cggcaggttt
     1441 tggtcaaccg caggggtatt cggttgtcgg tctggtttat gctttcatct ctttcgggcg
     1501 ggctgctgtt tgccgcatcg acagacggtg tgtcgtggac gaaaggtttg gcgatggctt
     1561 ccggcttcgg ttggtattcc ctctcgggtt tggtcatgac cgaggcttac ggcgcggtat
     1621 ggggcagcat catgctgctg aacgatttgg cacgagagct gtttgcactg gcatttatcc
     1681 cgctgctgat gaagcgtttt ccagatgcgg cggtgggggt tggcggtgcg accagtatgg
     1741 attttacatt gcccgtgatt cagggtgcgg gcggtttgga agtcgtgccg gtagcggtca
     1801 gcttcggcgt ggtggtcaat atcgccgccc cgtttctgat ggtggtgttt tccgctttgg
     1861 gttgaacgcg gtaaaatcgg catcccgatg caaggaggca gaaaacgatg aaaccgaaaa
     1921 tccaaaggca cggagagatt ttaagccttg tccgccggca tcagtttatg tcggtggacg
     1981 agcttgccgc cgcattggac gttaccccgc agacgatacg ccgcgacatc cgcgagttgg
     2041 aggaggtcgg cagcctgaaa cgccatcacg gcggcgcatc ttcgggcgga aacttgccgg
     2101 agggcctgcc cgccgaccgc caaacccggt gtcaaaacga aaaaaacgcc attgcccggc
     2161 tgattgcgga acacatcccc gacggttcgt cgctgtttgt cagtatcggc acgacgatgg
     2221 aggccgtggt gtcggagctg gtcaggcggc gcggcagcct gagggtgatt accaacaata
     2281 tccacgccgc ctcgattgct tcggcgcgga cggattatac ggtcatcatc acttccggag
     2341 tcgtccgccc tttggacggc ggtattaccg gcgtggcaac cgtcgatttt atcaaccagt
     2401 tcaaagtcga ttatgccgtg atgagtacgc acggcgtgga aagcgacggt tcgcttttgg
     2461 acgacgacta caaagaagtc agcgtgatgc aggcgatgat tgccaatgcg cgcgtccgct
     2521 tcctcggcgt ggaccacagt aaattccgca gcaacgcgct ggtcaggctc ggcgacatta
     2581 cggcgtttga caaagtattt accgaccggc tgcccgatac cgcgatgcag aagatgctga
     2641 aagaggcggg ggtggaatgc ttgattgccg atgccgtctg aacgctatgt caaagcgcgc
     2701 aagtcgggta caataaacac attcccaaac cgcttcagac ggcatacgga atcattccaa
     2761 tgccgtctga agccatctgt tcaaagaaaa ctatgctcaa taaagaccaa tttgcggaca
     2821 accattttat ccgcaccatc atcgaagaag acctcgaaag cggcaaacat acagctgtcc
     2881 aaacccgttt cccgcccgaa cccaacggct acctgcacat cggacacgcc aaatccatct
     2941 gcctgaactt cggtttggcg tatatttacg acggcttgtg caacctgcgt tttgacgata
     3001 ccaaccccga aaaagaaaac gacgaatacg tcaacgccat caaagaagat gtcgagtggc
     3061 tcggtttcca ttgggcgggc gagccgcgtt tcgcttccaa ctatttcgac cagctttatg
     3121 actacgccgt cggtttgatt aaagacggca aagcgtatgt cgatgatttg acgcccgaag
     3181 aaatgcgcga ataccgcggt acgctgaccg aagcgggtaa aaacagccct taccgcgacc
     3241 gcagcgtcga agaaaacctc gacctgttca cacgcatgaa aaacggcgag ttccccgacg
     3301 gcagcaaaac cctgcgcctg aaaatcgaca tggcatcggg caacatcaat atgcgcgacc
     3361 ccgtcatcta ccgcatccgc cgcgcccatc accacaacac cggcgacaaa tggtgtatct
     3421 acccgatgta cgactacacg cattgcatct ccgatgccat cgaaggcatc acgcattcct
     3481 tgtgtacgct cgaattcgaa gcacaccgtc cgctgtacga ctgtgtgttg gacaacattc
     3541 ctgcgccgca tgccacccgt ccgcgccaat acgagttttc ccgtttggag cttttgtaca
     3601 ccattacctc caaacggaaa ttgaatcagt tggttgtgga aaaacacgtt tccggctggg
     3661 acgatccgcg tatgccgacc atttccggta tgcgccgacg cggctacacg cccgaagggc
     3721 tgcgcctgtt tgccaaacgc gccggtattt ccaaatctga aaacatcgtc gatatgagcg
     3781 tgttggaagg cgcgattcgc gaagagttgg aaaattccgc cccacgcctg atggcggtgt
     3841 tgaacccgct caaagtgacc ctgaccaact ttgaaaccgg cagaacccaa agccgccgtg
     3901 ccgcgttcca tccgaaccac gaggaaatgg gcgagcgcga agtacctatt tcccaaacca
     3961 tctatattga agccgacgac tttgccgaaa atccgcccaa aggcttcaaa cgcctgattc
     4021 ccggcggcga agtacgcttg cgccacggct atgtcatcaa gtgcgatgaa gtcgtcaaag
     4081 acgaagcagg caatgtggtt gaactcaaat gcagcatcga ccacgacacc ttgggcaaaa
     4141 atccagaagg ccgtaaagtt aaaggcgtga ttcattgggt ttccgccgaa cacgccgccg
     4201 aaatcaaagt ccgtctgtac gaccgcctct ttaccgtcga gcgtcccgat gccgtgcgcg
     4261 gcgaagacgg cgaatacctg ccgttcaccg atttcctcaa tccggaatcc gtcaaggaaa
     4321 tcaccgccta cgccgaacct gccgccaaag atttgccggc agaaagccgt tggcagttcg
     4381 agcgtatcgg ctacttcgta accgaccgta aagaccacgg caaagacacg ccggtgttta
     4441 accgcacggt aacgctgaaa gactcttggc agcctaagta aaaccccatc cctgccgtct
     4501 gaatattgtt cgggcggaat ttctccttta cccgcgaaat gcggcacatt cggcacaccg
     4561 gaaaggaaat atgatgaaag tcctctttat cgccgacccg atggcaagtt tcaaaaccta
     4621 taaagacacc acctacgcga tgatgcgcga aatggcaaaa cgcggctggc ggctgtttca
     4681 cacattgagc ggggaattgt ctgtaaacgg cggtttggta acggcgcagg catcggcatt
     4741 tgaatttttg ggtgcaaaaa acgatgatga tcatgcgtgg ttcaaatctg ccgacaaagt
     4801 tcagacggca ttggaagcat ttgatgccgt gattatgcgt accgatccgc cgttcgatat
     4861 gcaatacctc tacgccaccc aattactgac gctggcggaa cagcagggcg cgaaggtctt
     4921 taacagcgga caggcgatgc gcgactttaa tgaaaaactg gcgattttga acttcagccg
     4981 ctttatcgcg cccacgctgg taacgacccg ttccgccgat gtccgcacat ttttgaaaga
     5041 acacggcgac atcatcatca aaccgctcga cggcatgggc ggtatgggaa ttttccgcct
     5101 gaccgaaaaa gaccccaaca tcggcagcat cctcgaaacc ctgatgcagc ttgattcccg
     5161 caccattatg gcgcaacgct acattcccga aatcgtacac ggtgacaaac gcatcctgat
     5221 tatcggcggc gaagtcgtcc cctatgcttt ggcgcgtatc ccgcaaaacg gcgaaacacg
     5281 cggcaacctt gcggcaggcg ggcgcggcgt ggcgcaggaa ttgggcggac gcgaccgcga
     5341 aattgccgaa acgctcgccc ccgaactcaa acggcgcggc atcctgctgg ccggtttgga
     5401 cgttatcggc agcaacctga ccgaagtcaa cgtaaccagc ccgaccggat tccaagaaat
     5461 tatgaaacaa aaaggtttcg atgtggcggc aatgtttgcc gatgccgttg ccgcgtggtc
     5521 ggtacgttaa accgatgccg tctgaaaggc ttttgcttca caaccgcctg gtttgattgg
     5581 gcaggcaggg ttttctctca gccggcaggg atacgcttaa taccgtacgg acaccccgcc
     5641 gccccgtttt cagacggtat atatagtgga ttaacaaaaa tcaggacaag gcg
//
LOCUS       DAWGRZ010000138         5551 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000138 DAWGRZ010000000
VERSION     DAWGRZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.138"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(43..810)
                     /locus_tag="WHT44_001646"
     CDS             complement(43..810)
                     /locus_tag="WHT44_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ2533803.1"
                     /translation="MSSNKASFFTRLRRLCRLAVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIRV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(898..1725)
                     /gene="mutM"
                     /locus_tag="WHT44_001647"
     CDS             complement(898..1725)
                     /gene="mutM"
                     /locus_tag="WHT44_001647"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ2533804.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLLIRFQTGVLLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYARLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPRCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(1777..2442)
                     /locus_tag="WHT44_001648"
     CDS             complement(1777..2442)
                     /locus_tag="WHT44_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ2533805.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRRLADVGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     VAD"
     gene            2621..4597
                     /locus_tag="WHT44_001649"
     CDS             2621..4597
                     /locus_tag="WHT44_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ2533806.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWGELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHKISASPQHTAAADGKRRVRLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4665..5357
                     /locus_tag="WHT44_001650"
     CDS             4665..5357
                     /locus_tag="WHT44_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEZ2533807.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHSLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLKNPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
BASE COUNT         1450 a         1785 c         1201 g         1115 t
ORIGIN      
        1 tccacaaaag cgaatcatac tgccacattc cgacaaccgg tttcagacag cgatatccgc
       61 atcgtcggcg acaacggcac ggatgctttc ttcgatttta tcttttaaag cataacggtc
      121 ttcgctttcc gccgcatccg ccacgcaaac gaaatcgact cttatcgtca attttttcat
      181 agacacgatg cgccacaggc aggtcggcaa accgacatcg gcatatgagg gacgagccgt
      241 cctttttccc gtttcgtcat aataacgcag cgcgaccgcc aaaacctttg cccccgcatc
      301 gatggcggat tggaacagcg cggctttgaa cggcaaaagc cccaatccgg aggaagtccg
      361 cgcttcgggg aaaaaactga cgttttgacc gcgttgcaag gtttcgcaga cggcgcggtt
      421 aatcggttcg atgtcgcgcc gcgaattgcg gttgatgaac accgttcccg cgttctgccc
      481 catcttgccc aataccggcc agcttttgat ttcctgcttg gcgataaagc tgctcggata
      541 aaccgcgctc atcgcgaaaa tatccagcca ggacacgtgg ttggcggcaa ccaagacacc
      601 gttcggatgt tcgggtgcgg gtctgcccac ctccaatccg atatccaaag ccgccaaaac
      661 ccccctgccc aactcgatta ccgcccgatt gcgcgactcg gggcaaccgc cgtcaatacc
      721 gcgcaggttt ttcccggttt tgaacagcca gaccgccaaa cggcacaagc ggcgcagacg
      781 tgtaaaaaat gaagctttat ttgaagacat gagcaattct ttcccgataa tcggtgtttg
      841 ttgttgaaac gttcggacgg cataccgata aaatgccgtc tgaaaccgtt ttcagtccta
      901 tttctgacag ttcgggcaat aaaacgtgcc gcgctgcccc aaagtttctt tcacaaccaa
      961 accgccgcac cgggggcacg gctgattgtg ccgcccgtac actgtatatt cctgttggaa
     1021 gtagccgctt ttgccgtcgc tgtccacaaa atccctcaag gtactgccgc ccgtttcaat
     1081 ggcgcgctgc aacaccgctt tgacggtttc aaccaaaagc gcgcactctt tctttttcag
     1141 gcggttggca ggacggtggg gcgaaatgcc cgctctgaac aggctctcgt tggcataaat
     1201 gttgcccaca ccgaccacga ccgcattgtc catcagggca agtttgaccg cgcgcttctg
     1261 cgccttcagc cttgcataca gataatccgc acaaaatgcc tccgacaaag gctccggccc
     1321 cagtttttcc aacagcggat gatgttcttc gattccctca taccaaagta tcgcgccgaa
     1381 ctttctcgga tcgcggtaac gcatgaccgt gccgtctgaa aacacaatat cgacgtgatc
     1441 gtgtctgtcc ggcctgccga tacgtccgtc cgacggcgta aaaatccgca agctgcccga
     1501 catccccaag tgaatcagca gcacgcccgt ttgaaagcgg ataagcaggt atttcgccct
     1561 cctgccgcag gacaacacct gccggccgga caaaatctcc cccaaatcgg gattaatctg
     1621 ccagcgcagc ttcaattggc gcaataccac ggcttccacc gttttccctt caatatgcgg
     1681 cgcgatgccg cgcaacgtcg tttccacttc cggcaattca ggcataaccc ctcccgacat
     1741 ttcttctgac agatgccgtc tgaaagacgg ctgtccctaa tccgcaaccc ttgccgcacc
     1801 cgccgcaagg gctttgccgc ccaaataccc gtcccacgcg ggcaggggcg ttacgcccgc
     1861 tgcccttttg accaaaacca aaccgtacac tgcggcacac tgcggccacc aacggtcgcc
     1921 cgccttttcc ataaaccgcc aaaagcgtat ttgcccgagc gacgaaaccg gcggcagata
     1981 caccataaat ttcccaaatt caatatcgaa accgacatcc gcaagccgtc ttttcaactc
     2041 gggcagcggc agacaaaacc gtttttccgg caggcgttcg ccgtcaaacc aacggctgaa
     2101 tccccagagc gaatacggat tgaaacccgt cagcatcaag cgtccgcacg gtttcaatat
     2161 ccggtgcgct tccgacagga tttgcgaagg aacaccgcct tcaagcgtat gcggaaaaag
     2221 cagcatatcc gcagaaacat ccgccaaagc catattctcc gccgacatcg acatatctcg
     2281 cggcacacag acaacatctt cagacaggct cagccacgga ccgcccacct gaaccgcaca
     2341 cattcccgaa aaacggtatg aatccagata ccgcccgaag aaatcctgtt ccaattttgc
     2401 aacataccgc cccatcgccg tatcttcaaa ccatgcatcc atattgcgtc cgtttcaaac
     2461 agattgcctg ccgatattct attccaaaca ggatttctgt caaaaaacac atcggccgcc
     2521 catttccgaa tccgcataaa gttcctgtaa aacttgacgc tttttcagtc aaacagtacc
     2581 atcggacgat aaaatatgtt tattccgccg aatataaatc atgtccaaac tcaaaaccat
     2641 cgctctgacc gcatcaggtc tgtccgtttg tccgggtttc ctatacgccc aaaacacctc
     2701 atcacaccaa atcggtttgg cgattatgcg cttaaactct tcaatactcg acctgccccc
     2761 gacaaaacaa tatttccaat ccggcagcct gtggggcgag ctgcgccaag gcttccggat
     2821 gggcgaagtc aatcccgaac tggtacgccg ccacgaaagc aaattcatcg caagccacag
     2881 ctatttcaac agggtcatca accggagtag accctatatg taccatatcg ccaacgaagt
     2941 caaaaaacgc aatatgcccg ccgaagccgc cctgcttccc ttcatcgaaa gcgcgttcgt
     3001 caccaaagcc aaatcacacg tcggcgcatc aggattatgg cagtttatgc ccgctaccgg
     3061 caggcattac ggcctggaaa aaacaccggt ttacgacggc aggcacgacg tttacgccgc
     3121 caccgatgcc gcactcaact atctgcaata cctctatgga ctgttcggcg actggccgct
     3181 tgcctttgcc gcctacaact ggggtgaagg caacgtcgga cgcgccatca accgcgcccg
     3241 cgcccaaggg ctcgaaccga cctacgaaaa cctgcgtatg cccaacgaaa cgcgcaacta
     3301 tgtccccaag ctgctcgccg tgcgcaacat tattgccact ccccaatctt tcggcatgaa
     3361 tatcagcgac atagacaaca aaccctattt tcaggcagtc gaaccggatc gtccgctcga
     3421 caacgaagcc atcgcccggc ttgccggcat cacgcaaagc gagctgctcg ccctaaaccc
     3481 cgcattcaac gtccccgcgt ttatccccaa aagcaaacgc aaactgctgc ttcctgtcgc
     3541 gtccgtacaa accttccaaa gcaactacct caacgccgca cccgacagcc tgttttcatg
     3601 ggaagtctat acgcctgccg ccaaaaccag cctgtccgac atctcgacgg caaccggcat
     3661 gagcattgcc gacatcaaac gcctcaacaa cctgaacggc aaccttgtca acgcaggacg
     3721 cagcatcctt gtcgccaaga acggcaaaac ccttcagacg gcatcggaat ccgtcgtttc
     3781 catcgacatc gacaatacgc ccgacaccta ccgttccaat atgccggcag gcacggtgaa
     3841 cgtcggcatt gcccgaatcc gacccgccgc cgcacagaca gcggacatta ccgtcgcacc
     3901 tttgccgcag aaaaccgtcc gtacggaacc cgatcccctt gtccgtattg ccgaacctgc
     3961 ccttgcgaca gccgcagcgc aacctcaaac cgaaaaacag accgccatgc cgtctgaaac
     4021 ccaaaccgca acactcgcgc agatcatccc ccaaaacgac atgcaggcgg cagacgaact
     4081 catgcagctt gttgcccgaa acaacctgcg ccgacaggct gaagaaacca tctccgccgt
     4141 catcggcacg cctgacacag ttgccgaaca caaaatttcc gcatctccgc aacataccgc
     4201 tgccgccgac ggcaaacgcc gggtacgttt ggaaacgcgc gtagccaaag ctgccgacgg
     4261 cgaagccgaa atctccccgc tccatgccag catccaccgc gttgtagaag gcgacaccct
     4321 gttcaacatt gccaaacgct acaacgtcag tgtagccgac ctgattgtcg ccaacaacat
     4381 caaaggcaac accatccaaa aaggacaggt actccgcctg gcgcaggcag cccctgccca
     4441 aacccgtatt gaaaaagtat cctacaccgc gcgcaaaggc gacaccttca aaagtatcgc
     4501 cgcgcgcttc aatatccata tcgacgacat ccgccggctc aatcccaacc tgaacaccat
     4561 caatccggga cagagggtca aactgattgg aagctgattc ggatacggca catgacagga
     4621 ctttctcagt cctgtctttt tcatcccaca tttcccatac catcatgaaa cttatcaaat
     4681 acctgcaata tcaaggcata ggaagccgca agcagtgcca atggctgatt gccggcggtt
     4741 atgttttcat caacggaacc tgcatggacg acaccgatgc agacatcgat tcctcatccg
     4801 tcgaaacgtt ggatattgac ggggaagcag taaccgtcgt tcccgaaccc tatttctaca
     4861 tcatgctcaa caagcctgaa gattacgaaa cttcgcacaa acccaagcac taccgcagcg
     4921 tattcagcct gttccccgac aatatgcgga acatcgatat gcaggcggtc ggcaggctgg
     4981 atgcagatac gaccggcgta ttgctgatta ccaacgacgg caaactgaac cacagcctga
     5041 cttcgccgag cagaaaaatt cccaagctgt acgaagtaac gctcaaacac cccacaggag
     5101 aaacgctctg cgaaaccttg aaaaacggcg tgctgctcca cgacgaaaac gaaaccgttt
     5161 gtgccgccga tgccgttttg aaaaacccga ccaccctgct gctgaccatt accgaaggaa
     5221 aataccacca agtcaaacgc atgatcgccg ccgccggcaa ccgcgtgcaa caccttcatc
     5281 gccggcgatt cgcacatctg gaaacagaaa acctcaaacc cggggaatgg aaatttatcg
     5341 aatgtccaaa attctgaaat aacatccaag aattccattt atattcatcc acatactcat
     5401 taaatatatg gtttaaccca atttaacgca aaaaatcaat ttacgatata atccatttgt
     5461 ctgagtaacg cctgttcaag caggcttatg agtaagacgt tttccccgta atgtgttttg
     5521 ccatctatac ttctcccctt gtatagatgg t
//
LOCUS       DAWGRZ010000139         5545 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000139 DAWGRZ010000000
VERSION     DAWGRZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.139"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            52..1455
                     /locus_tag="WHT44_001651"
     CDS             52..1455
                     /locus_tag="WHT44_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ2533808.1"
                     /translation="MNNPDLPYRQALECLSQKQYNFTEVRRLLTEAFSAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLIPLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALEWAKTSAKNNNPHGQYLLAQYCRYGTPPDFETA
                     HLLYRKSAAQGLPEAHWQLGLQYRFGQGTKVDTAQAVNHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDEAVHWFQQAAQENDPDAHAALADIYLQGKHLERNHKLALHHAEAAAAERHP
                     EGLRILGDICRYGLGIAPDTEKARHYYRQAAEAGSLSAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAERLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVEPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
     gene            1577..2293
                     /gene="trmB"
                     /locus_tag="WHT44_001652"
     CDS             1577..2293
                     /gene="trmB"
                     /locus_tag="WHT44_001652"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ2533809.1"
                     /translation="MTDTAENQTQNNWQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIDENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(2921..3283)
                     /locus_tag="WHT44_001653"
     CDS             complement(2921..3283)
                     /locus_tag="WHT44_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533810.1"
                     /translation="MSQLDFNLELFIKNSNFDDYKIFDIVKEAIETQDEIGSILRTHL
                     FFERILEAWIYAKCNKEDFFNDTQIGFRTKLKIARNLGLFSEVYDIGERLNKIRNTIA
                     HHVLREVNQENLTLYMKV"
     gene            complement(3396..5423)
                     /gene="uvrB"
                     /locus_tag="WHT44_001654"
     CDS             complement(3396..5423)
                     /gene="uvrB"
                     /locus_tag="WHT44_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ2533811.1"
                     /translation="MEVIQYPNSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRNDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTIFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIEK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEQGIVPQQIKKQVKDIIDGVYHEEDGSKGRLKGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRDIKENLLFGAE"
BASE COUNT         1396 a         1412 c         1381 g         1356 t
ORIGIN      
        1 ctaacttcat aacgaataac gatgattcga caaaacggaa aacgatctga catgaacaat
       61 cccgacttac cctatcggca ggccttagaa tgcctgtctc aaaaacaata taactttacc
      121 gaagtccgcc gactgctgac agaagcgttc tcggcaggtc atcccgccgc cgcattcgag
      181 ttggcaaaac acctgatgga cgcggacagc ccctaccaag accgcgaaca aggtatggaa
      241 atgctccgca tcgccgctga acagggacat ccctacgcgc gttacaatct ggcatatatc
      301 caagaattgg aaggcgcacc cccggaaacc ctgataccgc tttacagacc gttggcagaa
      361 gaaggactgc ccgaagcgca agtccgcctg atgtaccttc tgtacgcgtc ccgacatttt
      421 gaagaagcct tggaatgggc aaaaacaagc gcaaaaaaca acaaccccca cgggcaatac
      481 ctgcttgccc aatactgccg gtacggcaca ccgccggatt ttgaaacggc gcacctgctc
      541 taccgaaaat cggcggcaca aggcttgccg gaggcacatt ggcagctcgg gctgcaatat
      601 cgtttcgggc aagggacgaa agtcgacacg gcacaggccg tcaatcattt gcgcgccgcc
      661 gcacaacaag gatacattcc tgcctacacc ccacttgccg agctcatcct acctacggct
      721 cctgatgaag ccgttcactg gtttcaacag gccgcacagg aaaatgaccc cgatgcccat
      781 gccgcacttg ccgacatcta cctgcaaggc aagcatctgg aaagaaacca caaacttgcc
      841 ctgcatcatg ccgaagcagc cgccgccgaa cgccatcccg aaggtttgcg gatactgggc
      901 gacatctgcc gctacggttt gggcatagcc cccgatacgg aaaaagcccg gcattattat
      961 cggcaggcag ccgaagccgg cagcctttcc gcctatcaga aactcatatc cgacagcgcg
     1021 ttaaaccatc ctgaccaata cggcggcatt aaagattccg ccatcaggcg gcaaagggca
     1081 gaacggcttt atcaaaaagc ccaagccctg cattacggat tacaatgcgc gcccgaatac
     1141 gcagccgcgc tcaaactcta cacagaagcc gcagaactcg gacacagcaa agcccaaacc
     1201 aatctgggca gcatgtatta cttcggacag ggtatgaccg ccgactacaa tgaagcacgc
     1261 aaatggtttg aaaaagccgc cgcgaaaaaa gacagtatgg cgttctacaa cctcgcctgc
     1321 atccattaca gcggacacgg cgtcgagccg gacaaagaaa aagcctgccg ctacctgcaa
     1381 gaagccataa acaacggata cgggcaaaaa agcgtcctgc aagaactgct gcaacaatgg
     1441 caaaatgccg tctgaacagc gttacaccta ccctgccgaa acgaaacagg tataatcgcc
     1501 cctttccctt cccgccgtcc gaacaggcat ttcacattca gacggcatcc tgattgcaca
     1561 agcgtacgaa agcattatga cagacaccgc cgagaaccaa acacaaaaca actggcaagc
     1621 cggacacccc cgcagcatcc gcagcttcgt cctccgccaa agccatatga ccgccgcgca
     1681 gcaacgcgcc atcgatacct tatgggacag cttcggcatc gactaccaag caacaccggc
     1741 cgatcttgat gcccgtttcg gaagcagccg acccaaaatc ctcgaaatag gcttcggtat
     1801 ggggacggca accgcagaaa tcgcccgccg cctgcccgaa accgacttcc tcgccatcga
     1861 cgtacacggt cccggcgtag gcaacctgct caaactcata gacgaaaacc atttagaaaa
     1921 catccgcgtg atgcggcacg atgccgtaga agttgtcgaa aatatgctgc aagacggctc
     1981 gctcgacggc atccacatat tcttccccga cccgtggcac aaaaaacgcc accacaaacg
     2041 ccgtctgata caagccccct tcatcgccaa actactgccc aaactcaaaa ccggcggcta
     2101 tatccacctg gcgacagact gggaagaata tgcacagcag atgcttgaag tcctcagtag
     2161 cttcgacagc ctgcaaaata cggcggcaga ctacgccccc accccggact accgccccga
     2221 aaccaaattc gaagcgcgcg gcaaacgcct cggacacggc gtttgggact tggtattcaa
     2281 acggatcgga taacaaacca ctgtttgaaa atgccgtctg aaacatgttt gcttacagac
     2341 ggcatttttt caagataaag cagcaagtga tgtttcgata taaagtttaa aacaatagtt
     2401 tgaacggcaa aacgcgtgtg taccgcacgc atccttatag gttttatgca catcggtttt
     2461 aaagtttgtg ccgcccgcag gtagtatgtg atagctacgc acgcggttgg tgtgatgtag
     2521 gctacggctt gctggttaca accgtaaaaa agtaagtgcc gccattgcgg taaaaacgaa
     2581 gggatttcat agtgttatgc tcgtaatgat tttgtagatt ggattctcga atccgacctt
     2641 ttgggcattg ctgcaatgga ttgcaacgac gggaatgttg aaggttttgt cggatacaag
     2701 tatccgacct acgcttgttg ctatatatct tttgatttaa tgactaaata tgacaaagtt
     2761 aaagtgcaga ttacagcaag catgatatgc ttctttaggc ttttatcatt ccatgatata
     2821 gatatttctt ccttttcatt ttctttataa aattttaaac ctatatcacc atttttccat
     2881 tcctggtggt ttactatgat tttattttta aaagaatctc ttaaactttc atgtaaagag
     2941 ttaaattttc ttgatttact tcccttagta catggtgagc aattgtattt ctaattttat
     3001 ttaatctctc ccctatatca tatacttcgc taaataagcc aagattacgc gcaattttta
     3061 gttttgtgcg aaatccaatt tgtgtatcat tgaaaaaatc ttctttatta cattttgcat
     3121 atatccatgc ctctaaaatt ctttcaaaaa ataaatgtgt tcgtaagatt gaacctattt
     3181 catcctgtgt ttcaatagct tctttcacga tatcaaaaat cttataatca tcaaaatttg
     3241 aatttttaat aaataattct aaattaaaat ctaattgaga cataataagt gcccatttca
     3301 aaaataaatc tatattctag ttaatataat agttattcta atatctaaat taaataataa
     3361 actactattt ttatatccac gacaaagtct aagtctcact ccgccccaaa caacaaattc
     3421 tctttaatat ccctaatcct atcccgcaac acagccgcct cttcaaactg caaatcccta
     3481 gccgcctgct gcatggcttt ttcgagtttg gcgatttctt taatcgcatc ttcttcgttg
     3541 tgaatctcgc ccactttaac cttgttttta cctttcagac ggcctttact gccgtcttct
     3601 tcgtggtaca cgccgtcgat gatgtctttg acctgttttt taatctgctg cggcacgatg
     3661 ccctgttctt cgttgaattt aatctgtttt tcacggcggc gttcggtttc gtcgatagcg
     3721 gctttcatgg agtcggtaat tttgtcggcg tacaggatgg cgacgccgtt cacgttgcgc
     3781 gcggcgcggc ctatggtttg aatcaggctg cggtgggagc gcaggaagcc ttctttatct
     3841 gcgtcgagga tggcgacgag agagacttcg gggatgtcga ggccttcgcg taagaggttg
     3901 atgccgacga gtacgtcaaa caggccgagc cgtaaatctc taatgatttc aacgcgctcg
     3961 acggtgtcga tgtcgctgtg caggtagcgc actttgatac cgagttcgct gtaatagtcg
     4021 gtgagttgct ccgccatgcg tttggtgagg gtagtaacga gtacgcgttc gcctttttca
     4081 atgcggtcgt tgatttcgct cattaagtcg tcgacttggg tggcaacggg gcggatgatg
     4141 atttggggat caaccagccc tgtggggcgg acgacttgtt cgaccacttg tccggcgtgt
     4201 tcttcttcgt atttggcggg ggtagcggaa acgaagatgg tttgcggcat gactttttca
     4261 aattcgtgga atttgagcgg gcggttgtcg cgggctgaag gcaggcggaa gccgtagtcg
     4321 acgaggtttt gcttgcgcga tgcgtcgcct ttgtacatgc cgccgatttg ggttacggta
     4381 acgtggcttt cgtcgatgaa catgatggcg ttgtcgggca ggtagtccat cagcgtaggc
     4441 ggcggttcgc cttctttttt gccggaaaag tggcgggagt agttttcgat tcctttgcag
     4501 aagcccattt cgtagagcat ttcgaggtcg aaacgggtgc gctgttcgat gcgttgttgt
     4561 tcgacggggc gttgttcgcg ggcgaaaaat tcgatgcgtt cgcgtaattc ttctttgatg
     4621 gactcgcagg cgcgcaagac ggtgtcgcgc ggggtaacgt agtggctgga cgggaagacg
     4681 gtgtagcggc cgacgcgctg gataaggctg cctgaaagcg ggtcgaacat atcgaggcgg
     4741 tcgatttcgt catcaaacag gctgatgcgt aaggcgtttt cggagctttc ggcggggtac
     4801 acgtcaatca cgtcgccgcg cacgcggaag ctgccgcgtt tgaagtccaa atcgccgcgt
     4861 tcgtattgca tggaaacgag cgtggcgatg atgtcgcgct gctcgatggt atcgccttct
     4921 ttgacggaca acaccatttg ttgatactcg gtcgggtcgc cgataccgta aatggcggac
     4981 acggtggcga cgataatcac gtcgttgcgc gtcattaggt ttttggtggc ggaaaggcgc
     5041 atctgctcga tgtgttcgtt gatcgcgctg tctttttcga tgaacaaatc gcggctgggc
     5101 acataggctt cgggctggta atagtcgtag taggagacga aatattccac tgcgttttcg
     5161 gggaaaaatt cgcgcatttc ggcgtaaagc tgggcggcaa gggttttgtt gtgcgccatg
     5221 atgatggcgg ggcggccgct ttgggcgatg acgttcgcca tggtgtaggt tttgcccgaa
     5281 ccggttacgc cgagcagggt ttgataggca aggccgtctg aaagcccttc gagcaggcct
     5341 gcaatggcgg tgggctggtc gcctgcgggc gggaagggtt ggtggagttt gaagggggaa
     5401 tttgggtatt ggataacttc cataatcttg cctgtgatgc gtttgcggac aaagcgtgca
     5461 gtagggatgg gtcggaaacg tctttcagac ggcataaggc ggtgaaatcc tgaatgtatg
     5521 ccgtctgaaa cccaatcgct accca
//
LOCUS       DAWGRZ010000140         5544 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000140 DAWGRZ010000000
VERSION     DAWGRZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.140"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(227..3058)
                     /gene="lbpA"
                     /locus_tag="WHT44_001655"
                     /gene_synonym="tbpA"
     CDS             complement(227..3058)
                     /gene="lbpA"
                     /locus_tag="WHT44_001655"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ2533812.1"
                     /translation="MNKKHGFPLTLTALAIATAFPAYAAQAGGATPDAAQTQSLKEIT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVEYGIDRLDAFRQTYDIKRKTREPFFSVEGERESK
                     PVAKLAGYGKYLNNQLNRWVKERIEQNQPLSAEEEAQVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDKNLVRGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISESTG
                     FDENNQDKYLLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGILFKPNRHFSVSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADH
                     KTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(3055..5268)
                     /gene="lbpB"
                     /locus_tag="WHT44_001656"
     CDS             complement(3055..5268)
                     /gene="lbpB"
                     /locus_tag="WHT44_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin-binding protein LbpB"
                     /protein_id="HEZ2533813.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTPPPAGSSVETTPVNRPAVGAAMRLPRRNIASYKQDGTEIPDKHQAEEHLPLKEK
                     DILFLDGTLKEQADKLKKKINERYSDVRVITSKKEEEKYQYQFVRAGYVFTRAEGKDN
                     EKEKTSDGKEFVNRFSYDGFVYYSGERPSQSLPSAGTVQYSGNWQYMTDAKRHRTGKA
                     VSSTDLGYTTYYGNEIGATSYEARDADDREKHPAEYTVDFDNKTLNGKLIKNQYVQNK
                     SNPNEPKKPLTIYDITATLDGNRFTGSAKVSTEVKTQHADKEYLFFHTDADQRLEGGF
                     FGDNGEELAGRFISNDNSVFGVFAGKQKTETENAADTKPALSSGKHTKILDSLKISVD
                     EASDKNPREFAISSMPDFGHPDKLLVEGREIPLVNKEQTIELADGRKTTIRTCCDFLT
                     YVKIGRMQTERPAAKPKAQDEERDEEDTGVDSVEEGEDEIDDEEGTEDAAVKDEGSEE
                     DEAVEGEDEAEEPEEESPTEEGGSGSDGILPAPEAPKGRNIDLFLKGIRTAETDIPKT
                     GEAHYTGTWEARIGKPIQWDNQADKEAAKAVFTVDFGKKSISGTLTEENGVEPAFHIE
                     NGKIEGNGFYATARTRENGINLSGNGSTDPKTFQASNLRVEGGFYGPQAEELGGIIFN
                     NDGKSLGITEGTENKVDVEAEVDAEVDVGKQLESEVKHQFGVVFGAKKDMQEVEK"
BASE COUNT         1179 a         1497 c         1281 g         1587 t
ORIGIN      
        1 ttttgaaact ctaatcgcgt cattcccacg aaagtgggaa tccagttttt tgagtttcag
       61 tcattcccga taaattgcct tagcattgaa tgtctagatt cccgcctgcg cgggaatgac
      121 ggcggaaatc ttgtttatat tgaatcaaaa aaaacctgca ccttaatcag ttggcggttt
      181 agtccgactt ttggggtgca gatcaagctt tcagacggta tttcctttaa aacttcattt
      241 cgagcgcgag actgaagttc ctgcccggtg cggcatacct tccatagttg ctgtcgccgc
      301 cgtgccggtt tgccgtgctt tccgcagtct ggcgcaagga ttcccaagta acgtagcggt
      361 agttgccgat attgtagata gccgccctca aggtcagccg ttttttcaga ttcagatagg
      421 cggaaacgtc tgccgtcgac caagaagacg acgctctttt tgtcgaatat cgtttttgat
      481 cgcctgccag ataagcaagc tcgtcagggt ttttcccttt ggaataggtc agcataatgt
      541 ttgcgcccca tttcccctca ggctggtcgt atccgaaccc caaaacataa cgcgacggct
      601 gtaccgcatc caaagcatag ctgcggaggg acagtcccgg ccggttggat accgatttcg
      661 gtttgatgcg gttgtacgcc aatgtggtgt acaaaccttc gggcagtttg ccatacacgc
      721 cgttccagtc gatttttccc aatatattaa cgccttgaag cgacatattt tgggcattgt
      781 aataatcgcg tatatcaatc tctgtcaatt gtcctgcctg attcggcaat ttggttttgt
      841 gatcggcaac ggcaatcata tcggtataac ggttgcggaa gctgctgatt tccaaaaagc
      901 cgaaatcgcc cttccactgc aaaccgattt cccggttggc tgccttttcc gatttcaggg
      961 cgggacgctg ccagcctttc ggataatcgt gataaatgtc tatcccgaaa agttcttgga
     1021 atgagggcgt tctgaagccg ctggaggcac ggtaagacac ggaaaaatgc cggttcggtt
     1081 tgaacaagat gccgctgttc cacgaacggt caacataccg cccgctgcgg acgagttctt
     1141 ccgacgtggt gaagtttttc cggtcgtacc tgccgcccaa gctgaaatcg aaatatttgc
     1201 cgattgaaaa acggtcgttc aaagaaatat ggatattgct gccgttgatt tttcttggca
     1261 cgcatttgcg ggaacgcagg gtttcgatgt agccgcagac cgacccttcg acgacttcgg
     1321 gcttacccaa aagatactta tcttgattgt tttcatcgaa tcccgtggat tccgaaatcc
     1381 ttgccgcatt gtgggaaagc tgttcggggc gggaaatcgc tttggaagca tcgtaaccga
     1441 agcccaaagt cagatggtgt ttcgtccatt tgtttttcag cgatttctca aacgaggcat
     1501 tcaaaacatt gtgctgttcg cggtagtgga aacggtcgct gctgtcgtag gaatacggtt
     1561 tgtccgccga cgcgcggcag gatttgtcca cagcaggata cacggcgcaa ttcagcttca
     1621 gcgtgttgtt atcggttgcc acgccctgtt tgtcaaacga caacaccgcc ttatccgccc
     1681 aattgtcaga atacgcttcg ttttcataac gatacagcaa acccatacgg cggcggcggt
     1741 gatgttcgtc aataaatttg gtgcgggaat atttcaaacc tatgcccctg accaaatttt
     1801 tatcgccctt ccactcttct atattcggca caaaatacaa gccgtcgcgg aaatcgtcgc
     1861 cgtcgtacac cccgctcttg tctctaaact tttccgcctc gtccgtaccg taatactgtt
     1921 tttccgtcat atcgcggata tcgtaacgct gtttggtatc ctcaaacacg ccgccgacat
     1981 aatgcctgcc gccgaagcgg tagcccagct tggcaagcca agagccgctg cggtaatcca
     2041 tcggatcggg caatatcctg ccgccgcccg tgtaagcttg ggcggacaga ttttcgtggc
     2101 gcgcctgcgc ctcccgcacc tgcgcctctt cttcagcact taaaggctga ttttgttcaa
     2161 tacgttcttt tacccagcgg ttgagctggt tgttcaaata tttcccgtag cccgccaatt
     2221 ttgccacggg cttggattca cgctcgccct ctactgagaa aaatggctct cttgtcttgc
     2281 gtttaatatc gtatgtctga cggaacgcgt ccaaacggtc tatgccgtat tccaccccgt
     2341 ccgcaatatc gccgtgcggg cgcgtttccc gcccttggcg ttcggttcgg attaacagcc
     2401 cttcccaacc gtctttgctg aaccccgcgc cgagcgactt cataaattgg cggtttttac
     2461 tgccgtaggc ggtttttgcc tgtatccccc aacttttgcc gtctgaaatc aggtctgccg
     2521 cctctttggt gcggaaggcg accgcgccgc cgagtgcgcc gctgccgtga tcggacgaac
     2581 cggcaccttt gtcgatttcc accgtgctga tgttttcata ttcgatttcg ttgattgcac
     2641 cgctgccgcc gcgtccgccg tatccgctca acgatccctg cacggtaaac gcctgtattt
     2701 gggcaacacc gtcgaccgaa accgccacac ggtttttatc cacgccgcgt atcgagtagc
     2761 cgccgctcgc gccgttgccc tgttcgacaa ccgccacgcc cggatcgtag cgcgtcaggt
     2821 cgcggatacc gagtacctgt tctttgttca acgtttccga cgttttgacg attttgccca
     2881 aaccggtcgc ctctttcgat cgccgtccca ctttggcggc acggacggta atctctttca
     2941 gggattgggt ctgcgcggca tcaggtgtcg ccccccccgc ttgggcagca taagccggaa
     3001 aagcggttgc aatggccaag gcagtcagag tcagcggaaa accgtgtttc ttattcattt
     3061 ttccacctcc tgcatatctt tcttcgcacc gaataccacg ccgaattggt gtttaacttc
     3121 agattctaac tgtttgccaa catcaacttc agcatcaact tcagcttcaa catcaacttt
     3181 attttcagta ccttcagtta taccaagaga tttcccatca ttattgaaaa taataccgcc
     3241 caattcctcc gcctgcgggc cgtaaaatcc cccttctaca cgaagattac tagcttggaa
     3301 ggttttgggg tcggtcgaac catttcccga aagattgatg ccgttctccc gagtgcgtgc
     3361 tgtcgcgtag aaaccgttgc cctcaatctt gccgttttca atatggaaag caggttctac
     3421 accgttttcc tccgtcagcg ttccggaaat cgatttcttg ccgaaatcaa cggtaaatac
     3481 tgcttttgcc gcttctttat ccgcctgatt gtcccattga atgggtttgc cgatacgcgc
     3541 ttcccaagtg ccggtatagt gtgcttctcc agttttcgga atatccgttt ccgccgtgcg
     3601 gatacctttc aggaaaaggt cgatgttcct gcctttaggg gcttccggag cgggcaggat
     3661 gccgtctgaa ccgctgccgc cttcttctgt cggcgattct tcttcgggtt cttcagcttc
     3721 atcttcacct tctacggctt cgtcttcttc gctgccttcg tcttttacgg ctgcgtcttc
     3781 ggtgccttct tcatcgtcga tttcgtcttc gccttcttcg acgctatcaa cgcctgtatc
     3841 ctcttcgtcc ctctcttcgt cctgcgcctt cggtttggcg gcgggacgtt cggtttgcat
     3901 ccgtccgatt ttcacatagg tcagaaaatc gcagcaggtt cggattgtcg ttttcctacc
     3961 atcggcaagc tcgatggttt gttctttgtt taccaaagga atttcacgcc cttcgacaag
     4021 aagtttgtcg ggatgaccaa aatcgggcat agaggaaatg gcaaactcac ggggattttt
     4081 atcacttgcc tcgtcaacgg aaattttcag agaatccaag attttggtgt gttttccaga
     4141 cgacagggca ggttttgtat ctgctgcgtt ttctgtctct gttttttgtt tgcctgcgaa
     4201 tacgccgaat acgctgttgt cgttgctgat aaaccgtccg gcaagctctt ctccgttatc
     4261 gccgaaaaaa ccgccctcaa gccgctgatc ggcatcggta tggaaaaaca aatattcttt
     4321 atcagcgtgt tgcgtcttca cctcggtgct aactttggca ctgccggtaa agcggttgcc
     4381 gtccaatgtt gcggtaatgt cgtaaatggt cagcggtttt ttgggctcat ttggattact
     4441 tttattttgc acatactgat ttttaatcag cttgccattc agggttttgt tatcaaaatc
     4501 aaccgtatat tcggcaggat gcttttccct gtcgtcggca tccctagcct cataagaagt
     4561 tgccccaatt tcattaccat aatatgtggt ataacccaaa tccgtactgg aaaccgcctt
     4621 acctgtccga tgacgtttgg catcggtcat atattgccag ttaccggaat attgcaccgt
     4681 tcccgcgctc ggtaaagatt gggaaggacg ttctccggaa taatatacaa aaccgtcata
     4741 actaaatcgg ttaacaaact ccttaccatc agaagtcttt tctttttcat tatcctttcc
     4801 ttccgccctg gtaaacacat agcccgcacg gacaaattga tattgatatt tttcttcttc
     4861 ttttttcgat gtgataaccc tcacatcaga ataccgttcg ttgattttct ttttaagttt
     4921 gtcagcctgt tctttcagcg taccgtctaa aaacaggata tccttctctt taagcggcag
     4981 atgctcctct gcctgatgct tgtcgggaat ttccgtaccg tcttgtttat aggaagcaat
     5041 attccgcctt ggcagccgca ttgccgcacc gacggcgggc cggttgaccg gcgtggtttc
     5101 taccgaagac ccggcagggg gcggagtggg aacgtcctta gatttgaagg tgacggggta
     5161 cgcggtcggc gttgattcga caacaggctg cacgccgaaa ttgccgccga tacaagatgc
     5221 taaaagtaag ggcaacaaga caatgccgcc ataattcggt ttacacatcc ctacttttcc
     5281 tctatttgat taataataat tatcattata ttaatatgta cagataatat caagccgttt
     5341 ttatagtgaa ttaacaaaaa tcaggacaag gcgacgagcc gcagacagta cagatacatt
     5401 ccgtcattcc cacgaaccta catcccgtca ttcccacgaa cctgcaccac gtcattccca
     5461 cgaaagtggg aatccagttc gttcggtttc gcttgtttta agtttcgggt aacttctact
     5521 tcgtcattcc cacgaacctg catc
//
LOCUS       DAWGRZ010000141         5490 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000141 DAWGRZ010000000
VERSION     DAWGRZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.141"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            69..494
                     /locus_tag="WHT44_001657"
                     /pseudo
     CDS             69..494
                     /locus_tag="WHT44_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            500..1729
                     /locus_tag="WHT44_001658"
                     /pseudo
     CDS             500..1729
                     /locus_tag="WHT44_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            1787..2407
                     /locus_tag="WHT44_001659"
     CDS             1787..2407
                     /locus_tag="WHT44_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ2533814.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVNQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLIGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            2418..2549
                     /locus_tag="WHT44_001660"
                     /pseudo
     CDS             2418..2549
                     /locus_tag="WHT44_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2546..3040
                     /locus_tag="WHT44_001661"
     CDS             2546..3040
                     /locus_tag="WHT44_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ2533815.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(3128..4789)
                     /locus_tag="WHT44_001662"
     CDS             complement(3128..4789)
                     /locus_tag="WHT44_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ2533816.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEVGAHSLAGAVIDPIALNELISDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGILVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(4919..5461)
                     /locus_tag="WHT44_001663"
     CDS             complement(4919..5461)
                     /locus_tag="WHT44_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ2533817.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVVTLLNNFYGQKLTEEDVL
                     KKLDKEQMPASFEDMRRIMPDLGFEAKGYALSFEQLAQLQIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKGRDAGGDKAFFT
                     RSPKRQTEFAVGQVKWWRAY"
BASE COUNT         1311 a         1486 c         1398 g         1295 t
ORIGIN      
        1 atattatccc gtatgcggat tggttttaag atttgtaaat ttgatttgca tcaaaaaatc
       61 gccgatagat gattcatata atatcaatat taaagagtat cggtatatcg gggatagtca
      121 tgtcctgttt ttcaatcaaa cgtatgtccg cgtttcgggc gcggataacg gcgttttttg
      181 ccgcctttgt ctttttgacg gcggcactgc ccgcttatgc ggagcgtctg cctgattttc
      241 tggcgaaaat acagccttcg gaaatttttc cgggtgcgga ccgttacggc aagccggaag
      301 gtaagcctat ggttgcccgc gtttacaaag gcgatgagca gttgggcttg gtctatatca
      361 cgaccgatgc ggtcaatacg cgcggttatt cgagcaaacc gattgatacg ctgatggtgt
      421 tggcaaacga cggcacgata gccggggcga aactggtcga ccatcacgaa ccgattatgc
      481 tgatcggtat cccgcatttg cccgcgcccg ggcgggcgat acgctcaaac tggcttccgg
      541 cgtatataaa accaaacttc acattgacaa accgattacg attgaagggc ctgccgaccg
      601 ttccgcaacc atcgaaggcg acaggagcgg gcgtaccata gccgtacacg cgccggacgt
      661 aacgctccgc aacctgaccg ttacccgttc cggtatgagc ctgcccgcaa tggatgccgg
      721 tatttatctc gaagaaactg ccccgcgcgc cctgattgaa cacaacaata ttttggataa
      781 ttcggtcggc gtatatctgc atggttctgc cgatgcgatg gtgcgcgaga ataaaatcgt
      841 cggcgacgcg actttgcgcg tgaacgagcg cggcaacggc gttaccgttt ggaacgcacc
      901 cggtgcgcag gtcgtcggca acgacatttc caaagggcgg gacggcattt tttccaatac
      961 cagcacgcac aacacctaca aaaacaaccg cttcagcgat ttgcgtttcg ccgtccacta
     1021 tatgtacacc aacgacagcg aaatcagcgg caatatttcc gtgggcaaca atatgggcta
     1081 tgtgctgatg ttttccgagc ggctcaaagt attcgacaat atcgccgtcg gcagccgcga
     1141 tcagggcatt atgctcaact atgtcaacta ttccgatatt cacgacaaca ttatcaacaa
     1201 ggcaggcaag tgcgtatttg cctataatgc caactacgat aaacttttcg ccaatcattt
     1261 tgaaaactgt caaatcggca tacactttac cgccgccatc gaaggcacgt ccttgcatga
     1321 caattccttt atcaacaacg aaagccaggt caaatacgtc agcacgcgct ttctcgattg
     1381 gagcgagggc ggacacggca actattggag cgacaacagc gcgttcgatt tgaacggcga
     1441 cggcttcgga gacagcgcgt accgccccaa cggcatcatc gaccaaatca tctggcgcgc
     1501 gcccgtatcg cgccttttga tgaacagtcc cgcaatcagc atcgtcaaat gggcgcaggc
     1561 gcagtttccc gccgttctgc ctggcggcgt ggtggacagc aaaccgctga tgaagcctta
     1621 tgcccccaaa attcaaaccc gttatcaggc gatgaaggac gagctactca aagaagtcga
     1681 aacgcggcag tcggaatggg gcagggcgga aaacggttct ttgaactagt ctgcttcaga
     1741 cggcatccgg attcaaatgc cgtctgaaaa cacaaaagga acaaccatga ccacacatca
     1801 tgtcgaattg aggaaggtaa ccaaacggtt cggggcgcaa aaagccgtca accaagtcga
     1861 tttggttttg aaggcaggag aaagcgtcgg gcttgccgga cacaacggcg cgggcaagtc
     1921 caccattatg aagctgatac tcgggctgat taccccgacc gaaggcgaag tgatgctttt
     1981 gggcgaacgt accggtagca aagcgggggc gcggcttcgc agccaaatcg gctacctgcc
     2041 cgaaaccgtt gcgctgcacc cttcgctgat cggcatcgaa acgctggatt tttatgccaa
     2101 acttaaaaaa cagccgctca cgcagaaccg ggggctgctt gagcgcgtcg gcatttcaca
     2161 ggcggcacac cgccgcgtcg gcacttattc taaagggatg cgccaacgcc ttgccttggc
     2221 acaagccctg ctgggcgagc ccaaagtcct gctgtttgac gaaccgacaa ccggtcttga
     2281 ccctgcatca cgacaaatgt tttacgaagt cgtgcgcgaa ctcaacgggc gcggcgcgac
     2341 cgtattgctc agcacccacg cccttgccga gttggacggg cacgccgacc gcattatcgt
     2401 ggattaaatt taatccacta tatgcgggta tggcgggttt gagcggacaa atcagcctga
     2461 ccgtccccgt tttgctgacc gctcaggttt tatgggttat cattccgctt gttttggcag
     2521 ccggaatttt tagaaagcga caaatatgaa aaaaaccctg ttggcaattg ttgccgtttc
     2581 cgccttaagt gcctgccggc aggcggaaga gggaccgccg cctttacccc ggcagattag
     2641 cgaccgttcg gtcggacact attgcagtat gaacctgacc gaacacaacg gccccaaagc
     2701 ccagattttc ttgaacggca aacccgatca gcccgtttgg ttctccacca tcaagcagat
     2761 gttcggctat accaagctgc ccgaagagcc taaaggcatc cgcgtgattt acgttaccga
     2821 tatgggcaat gttaccgatt ggacgaatcc caatgccgac acggagtgga tggatgcgaa
     2881 aaaagccttt tacgtcatcg acagcggctt tatcggcggt atgggtgcgg aagacgcgct
     2941 gccgttcggc aacaaagagc aggctgagaa atttgcaaag gataaaggcg gtaaggttgt
     3001 cggtttcgac gatatgcctg atacctatat tttcaaataa tattgaaaaa accggccgaa
     3061 actttaaaaa cgagtttcgg tcggtttttc ttttcttgtt cggtatagtg tcggcaggaa
     3121 agaaccttca catcccgccg taattcggcc cgctcgcgcc ttcggggcaa atccaagtga
     3181 tgttttgcgt cgggtctttg atgtcgcagg ttttgcagtg cacgcagttt gccgcgttga
     3241 tttgcaggcg cggattgccg ttttcttcaa caatttcgta cacgccggcc ggacaatagc
     3301 gcgtttcggg cgaggcgtat tctttgtagt tcacgtctat catcgtttgc ggattgttca
     3361 gcaccaaatg gtcgggctgg ttttcttcgt gcgcgagatt ggcaaggaag acgctgctca
     3421 agcggtcgaa ggtcaacacg ccgtcgggtt tcggataatc aatcggctta cacgcggcgg
     3481 cttttttaag ctgctcgttg tctttgccgt gatgtttcaa ggtccacggg gctttgcctc
     3541 tgaaaatcat ctgatcgatg ccggtgtaga ttgagccgag gtaaacgccc catttgaatg
     3601 acggacggac attgcgcgcg gcgtaaagct cttgatacag ccagctttgt tcaaaacgtt
     3661 gctgataatc cgccgcctct ttgccgctgt cgaaaccctc cacttcttca aggttttcca
     3721 acaaggggaa cacggcttcg gcggcgagca tggcggattt catcgcggta tgaatgcctt
     3781 tgatgcgcgg catattgagg aaacccgccg catcgccgac caaaatgccg cctttgaacg
     3841 agagcttcgg caaactttgc aaaccgcctt caatcagcga acgcgcgccg taagcaatgc
     3901 ggcggccgcc ttcaaaggtt ttgcggattt cgggatgggt tttgaaacgt tggaactctt
     3961 caaacggcga cagataagga ttttgatagt ccaaaccgac cacgaagccg acggcgactt
     4021 tgttgtcgtc gaaatggtaa acaaacgcgc cgccgtaggt tttgctgtcc agcggccagc
     4081 ctgcgctgtg caccaccaaa ccgggctgat gctgttcgga cggcacttcc caaacttctt
     4141 taatgcccaa gccgtaagtt tgcggctggc tgttttggtc gagttggaaa cgttcgatga
     4201 tttgtttgga aagcgaaccg cgacaacctt cggcaaacag ggtttgctgc gcccaaagct
     4261 ccatgccggg ttggaatgaa tcggtcggct cgccgtcttt gccaatgccc atattgccgg
     4321 ttgcaatgcc tttgaccgaa ccgtcttcgt gatacagcac ttcggcggcg gcaaagcccg
     4381 gatagatttc cacgcccata ttttccgcct gctccgccaa ccagcgcacg acttcgccca
     4441 agctgacgat gtagttgccg tgattgtcga aattcggggt aatcggcagg ttgaacgctt
     4501 ttttctcggt caggaacaac actttgtcct gcgttactgt gcgtgtcagc ggtgcgcctt
     4561 tttctttcca gtcggaaatc aactcattca gcgcaatcgg atcgataact gcgccagcca
     4621 gcgaatgcgc ccccacctcc gaacctttct ccaccacgca aacgctgatt tcgcgcccgt
     4681 tttgttcggc aagctgcttg agtttgatgg cggcagacaa acccgacggg cctgcgccga
     4741 caatcacgac atcgtattgc atactgtcgc gggtgatgga ttctgtcatg gcggttcctg
     4801 tgtatttatt attgaattgc aaatccgtaa ttatacaacg ggaacatata gttaccaaat
     4861 acaacaaagg tcgtctgaaa accatatttt cggttttcag acgacctttg tcgaaatttc
     4921 aataagcacg ccaccatttt acctgtccga ccgcaaactc cgtctgacgt ttcggactgc
     4981 gtgtgaaaaa cgccttatcc ccgccggcat ccctcccttt cggcacaacc gccaaaatct
     5041 tacctgccaa atttccctca cgggtttgcc aagcatccaa aaactgcgcc ctgctcattg
     5101 aaacatgacc cagcgacggg tcggcaagca aaaccgtatt gccgtctata ccgcgcaata
     5161 ccgagaaatg atcatccttg cggtatttca gatacacgat gacggggatt tgcaactgtg
     5221 caagctgctc gaaagacagg gcatagcctt tcgcttcaaa acccaaatca ggcataatgc
     5281 gccgcatatc ctcaaacgac gcgggcatct gctccttatc cagttttttt aacacgtcct
     5341 cttccgtcag cttttgcccg taaaaattgt tcaaaagcgt caccaccgaa gccgccccgc
     5401 aggaaaaatc caaatcctgc tttacaatat tgaaatcgcg cctttctttc caactctgca
     5461 ctttgatttt tccataagca acaggattat
//
LOCUS       DAWGRZ010000142         5429 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000142 DAWGRZ010000000
VERSION     DAWGRZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.142"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(78..278)
                     /locus_tag="WHT44_001664"
     CDS             complement(78..278)
                     /locus_tag="WHT44_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533818.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALREVAEISAWVLLG
                     AAAAMLFWYLFMLYFKHIPDSY"
     gene            428..1567
                     /locus_tag="WHT44_001665"
     CDS             428..1567
                     /locus_tag="WHT44_001665"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ2533819.1"
                     /translation="MSQNASVGIVMPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIEQWAAIVGGSLGGMQA
                     LQWTISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGFGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGDSLTRALQNVKAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            1564..2145
                     /gene="metW"
                     /locus_tag="WHT44_001666"
     CDS             1564..2145
                     /gene="metW"
                     /locus_tag="WHT44_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ2533820.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTNSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRILERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            2262..3098
                     /locus_tag="WHT44_001667"
     CDS             2262..3098
                     /locus_tag="WHT44_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ2533821.1"
                     /translation="MKNKTSSLLLWLTAIMLTACSPSKDDKTKEVGASAASSSASSAP
                     SQTDLQPTASAPDNVKQAESAPPSNCTSLHPATGIDDLMQQIAEHIDSDCLFALSHHE
                     LETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQNALME
                     QERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYISQL
                     PGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNPNTG
                     RI"
     gene            complement(3149..3709)
                     /gene="efp"
                     /locus_tag="WHT44_001668"
     CDS             complement(3149..3709)
                     /gene="efp"
                     /locus_tag="WHT44_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ2533822.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(3752..4900)
                     /gene="earP"
                     /locus_tag="WHT44_001669"
     CDS             complement(3752..4900)
                     /gene="earP"
                     /locus_tag="WHT44_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ2533823.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDTEALRERLMLPEKNASEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDQLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETVSAHRRLSDDLNGGEALSAT
                     QRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSAPEKLAAFVSKHQKIR"
     gene            complement(4919..5197)
                     /locus_tag="WHT44_001670"
     CDS             complement(4919..5197)
                     /locus_tag="WHT44_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533824.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFVANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIILLIVYLFHYFGAF"
BASE COUNT         1376 a         1400 c         1419 g         1234 t
ORIGIN      
        1 cacctcctac cgcaagccct tttcatcaaa tatgccgtct gaaacagaaa aatgcgtttc
       61 agacggcatt ttttccgtca atacgaatcc ggaatgtgtt tgaaatacag cataaacaga
      121 taccaaaaca gcatcgcggc ggctgcaccg agcagtaccc atgcgctgat ttcggcgact
      181 tcgcgcaggg cgcgtttttg cctgcggctg aaaaggcggt tgataatcaa cgcggcaagt
      241 gcgaaaaaga aaaaaatacg gaaaagtctg cctatcatgt cgggaagcgg tggaggtcgt
      301 ctgaaaaagt atgattcgtg tgcggcggat gagggtcaaa cgcgtccggc tgcttgtccg
      361 accgtgtcaa aaagtcatgc cgtttgcgat ataataacgc ttttcgccgc atttgttgga
      421 acaggatatg agtcaaaatg cctcggtggg cattgtaatg ccccaaaaaa ttccgtttga
      481 gatgccgctg gttttggaaa acggtaaaac tttgccgcgt ttcgatctga tgattgaaac
      541 ctacggcgag ctgaatgctg aaaaaaacaa tgcggtttta atctgccacg cgctgtcggg
      601 caaccatcat gttgcgggca ggcattcggc ggaagataaa tatacgggct ggtgggacaa
      661 tatggtcgga cctggcaaac cgattgatac ggaacgtttt ttcgtggtcg gtttgaataa
      721 tctgggcggc tgcgacggca gcagcgggcc tttgtcgatt aatcctgaaa cgggcaggga
      781 atacggcgcg gattttccgg tggttacggt gaaggactgg gtaaaatccc aagccgcgct
      841 tgccgactat ctcggtatcg aacaatgggc ggcgattgtc ggtggcagct tgggcggtat
      901 gcaggccttg cagtggacga tttcctatcc cgagcgcgtg cgccatgctt tagtgattgc
      961 gtctgcgccg aaactgtcca cgcaaaatat cgcgtttaac gatgtggcgc gtcaggcaat
     1021 tttgaccgac cctgatttca atgaaggaca ttaccgcagc cacaataccg ttcctgcacg
     1081 gggcttgcgg attgcccgca tgatgggaca catcacttat cttgccgaag acggtttagg
     1141 caaaaaattc ggacgcgatt tgcgttccaa cggttatcaa tacggctttg gtgttgaatt
     1201 tgaagtggaa tcctatcttc gctatcaggg cgacaaattc gtcggacggt tcgacgccaa
     1261 cacttacctg ctcatgacca aggctttgga ctatttcgat ccggcggcag atttcggcga
     1321 cagcctgacc cgcgccctgc aaaatgtgaa agcgaaattc tttgtcgcca gcttcagcac
     1381 cgactggcgt ttcgcacccg aacgttcgca cgaactggtc aaggcactga ttgccgccca
     1441 aaaatccgtg cagtatatcg aagtcaaatc cgcacacggg cacgatgcct ttttaatgga
     1501 agacgaagcc tatatgcgtg cggtcgccgc ctatatgaac aacgtttata aggaatgtca
     1561 gcaatgaacc tgcgcgatga tttgcaactg atttacgacc ggatacccga aggcagccgc
     1621 gtcttggact tgggctgcgg cgacggcgaa ctgcttgccg cattggtcga acacaaaaaa
     1681 tgcagcggct acggcatcga aatcgacaca aatagcgtca tcgccgcgat gtcgcgcggc
     1741 gtaaatgtta tccaagccga tttggaagaa ggtttaaccg cattcaacga tcaaagtttt
     1801 gatgtgattg tgttgagcca aaccatccaa gcgatgcaga ataccgaaaa aatcctgcgc
     1861 tgcctgatgc gcgttgccaa acaggcgatt gtcagcttcc cgaatttcgg ctactggcgc
     1921 aaccgcgtcc aaatcgcact cggcggacat atgccggttt ccgaacgtat gccctaccat
     1981 tggtacgaca cgcccaatat ccattggtgc acgctcaaag actttgattt attgtgcgcc
     2041 aaaaacaaca tccgcatcct cgagcgcgcc gtgatgacgg gcaacagaca ggtcaaacat
     2101 ttccccaacc ttttgggcag cctggcgttt taccgcgtcg ggtaagacag gttgagaacc
     2161 ttgtcggcat taacaggttc ttcggttcta ttaacaacag gccgtctgaa aaatgaaacg
     2221 gtaggttttc agacggcctc tttaatactg gaaacaatag aatgaaaaac aaaacctcat
     2281 cacttctctt atggcttacc gcaatcatgc tgaccgcgtg ttctccgagc aaagacgata
     2341 aaaccaaaga agtcggtgca tccgctgctt cgtcctccgc gtcatcagct ccttcccaaa
     2401 ccgatttgca accgaccgca tccgcccctg ataacgtcaa gcaggcagaa agcgcgccgc
     2461 cgtcaaattg caccagcctg caccccgcca ccggcattga cgatctcatg cagcaaatcg
     2521 ccgaacacat tgactcggac tgtctgtttg ccctttccca tcacgaactg gaaacccgtt
     2581 tcggcttacc cggcggtggc tatgacaaca tacagcggct gctgtttccc gacatccgcc
     2641 ctgaagatcc cgactaccat cagaaaatca tactggcaat tgaagacttg cgttacggaa
     2701 agcgcacgat cagccggcag gcacaaaatg ccttgatgga acaggaacgc cgcctccgag
     2761 aagcgacgct gttgctgata cagggcagtc aagaaacccg cggacaaggc gaggagccga
     2821 aacgcacgcg ttattttgaa gtttcggcaa cccctgccta ttcgagccgg cacaacaacg
     2881 gacttggcgg caatttccaa tacatcagcc aattgcccgg ctatctgaaa atacacggag
     2941 aaatgcttga aaaccaatca ctcttccggc tgtccaaccg tgaacgcaat cccgacaaac
     3001 cgtttttaga catccatttt gacgaaaatg gcaaaatcac gcgtattgtc gtttacgaaa
     3061 aaaacatcta cttcaatcca aacacggggc gaatataaaa aaaatgccgt ctgaaacgtt
     3121 ttcagacggc atttttcaat gcggcaaatc aggcgcgttt gcggaattcg ccggtgcggg
     3181 tgtcgatttc gactttatcg ccgttttcga tgtaagacat cacttggatt tcggtgccac
     3241 cgaccaggcg ggcggttttc atcactttgc cggaagtatc gcctttgacg gcaggctcgg
     3301 tgtactcgac ttcgcgcacg atgatggtgg gcagttctac ggagataggg ttgccttcgt
     3361 agaaggttac ttcgcattgg tcttccatac cgtcaacgat gaatttcaac gcgtcgccga
     3421 tgttgtcagc ttcgatttcg tattggttga attcttcgtc cataaagacg tacatcgggt
     3481 cggcaaagta gctgtacgta cagtttttgc gggacaggat gaccacgtcg aatttgtcgt
     3541 cggctttgta aatggtttcg gaagccgcgc cggtcagcag gtttttcagt ttcatgctga
     3601 ctttggcgga agagcggccg cctttgatgt attcggtttt ttgaacgacc ataggatcgt
     3661 tgccgaccat aaatacattg ccggcgcgca gttcttgtgc tgttttcatt gtgattttcc
     3721 aatcaaatcg gttgtcgtaa aacgcgctat tctagcgtat tttttgatgc tttgaaacaa
     3781 aggcagcgag tttttcagga gctgacggct gcccgaaaag ataacggctc caatcctccg
     3841 cgccttgccg ccagccgttt tgatgttgtt gcagggtttg ccaacattcg aggcgttgtg
     3901 ttgcggataa agcctctccg ccgttgaggt cgtccgaaag acggcggtgt gccgacacgg
     3961 tttcgggcgt gtagaaaccg tgtgccttat cccaaaaggc gtggagtttg tcgagatgga
     4021 cattctcgtc ttgcgggtag atgtgccaaa agaagggttt gcccgcaagc tgggcgcgca
     4081 cgaaactgtc ttcgccgcgg atgacggcgc agtcggcaag gtgcagcagt tggtcgaagt
     4141 cctgttgcgg cacgaaaggg attttgacga ggcggacgga tgccgtctga aaaacatcgc
     4201 cgtcgttttg cagggcatct tgcggaataa cgccgctttg tttgaggctg tcgatgattt
     4261 gcgtccccgc cagcaacagt gtcatcgggc tgcctgcctg tcgccacatt tccagccact
     4321 ttgcccaaac atcgctccga tagccgaaaa gcagccattc ggaggcgttt ttttcgggca
     4381 gcatcagccg ctctcgcagg gcttcagtat cgaaacggac ggcttcgcag taatcacgtt
     4441 cgcgtatcaa cccgccgctt ttttcgctga aacccataaa ccaaaaatat ttttgaacac
     4501 cctcctgcgg cgaaggcatc agatgcagcc tttcattgct ttcctccgcg ctcaaatatt
     4561 cccaattcag ccaaagcggc ttgtgtcggc ggataatgtg cagcacattt tcgggcaggt
     4621 cgcaggcaaa agtttcgatg acgacatcgg gaacaggcgc ggtatcaata tctgccgcat
     4681 cggaatgcca agtgcggaca tgaatatcct gatgaacgca gggaacatcg ggcaaatcag
     4741 ggcaaagcgc acgcaaggcg gacacatcgt ccgtccacaa atgcacctgc caaccgagtt
     4801 cgcggtgcaa aacacgggcg agccgccacg aaacgccgat gtcgccgaaa ttgtcgatga
     4861 ccttgcaaaa aatccaacag acaaaaggag gagtattcat agaaataacg gaaacgggct
     4921 aaaacgcgcc aaaatagtgg aacaaataca caatcagcaa aataatcgga ataaacagca
     4981 acatagtttg acgtttcaaa tcaaactctt gtttcaaatg atgcttgatg gtgtcgaaaa
     5041 ggacgaattt gaacaaaaac gtcgcttcgc aggcaaggat gatgccgtta atcaaatgct
     5101 ggggtttgtc ttcgggcgga ttggcgacga aaacggcaag cgtcgccaag gggatgaggc
     5161 agacgacgag ccagcggaca agcaggtttc gggacattgc aaggctttca aaaaagcaaa
     5221 acgccattat cgccccaagc gcacgccttg tctaacggac gcaccagaga tgccgtctga
     5281 agccttgccg caggaaaacg ccgcaccgcc gaacctttaa atacgcccgg gacgggcatt
     5341 ccccaaaaaa tcggacactg ccgctgatgc ctgcggacaa ggcggcatcg ggcgcgggca
     5401 tattgaaaac ccggcgcgtc ggtttgaaa
//
LOCUS       DAWGRZ010000143         5403 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000143 DAWGRZ010000000
VERSION     DAWGRZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.143"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(247..1506)
                     /gene="rho"
                     /locus_tag="WHT44_001671"
     CDS             complement(247..1506)
                     /gene="rho"
                     /locus_tag="WHT44_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ2533825.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEAAEFLIGKIKASKNNDDFFELMRGK"
     gene            complement(1726..4110)
                     /gene="ppsA"
                     /locus_tag="WHT44_001672"
     CDS             complement(1726..4110)
                     /gene="ppsA"
                     /locus_tag="WHT44_001672"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ2533826.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTAEAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLDNVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGNRNLRRFAINGDKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4522..5343
                     /locus_tag="WHT44_001673"
     CDS             4522..5343
                     /locus_tag="WHT44_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ2533827.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASGQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
BASE COUNT         1141 a         1266 c         1588 g         1408 t
ORIGIN      
        1 acaaaaatga gtgcggttcg gcgcaacctt gaatcaagtt cccgcatcgg ttttcattgc
       61 cggtacggat gcgttcaagc cggctttgca aaggccgcgc tttcggcaag cggacacgga
      121 cactgccgac ggttgcgccg ttaacggggg gagggaggag ctgcgccgac cgtgtgaatg
      181 aaagtgccgt ctgaaacccg attttcaggc ttcagacggc atttcgcatt aatgcgggcg
      241 gcgcgtttat ttgccgcgca tcagttcaaa gaaatcgtcg ttgtttttag aggctttgat
      301 tttcccgatt aaaaattcgg ctgcctcgat ttcgtccatc gggtgcagga acttgcgtaa
      361 gagccacata cgttgtaact ggtcgtttgg gacaagcagc tcttcgcggc gcgtgccgga
      421 tttgttgatg ttgatggcgg ggaagaggcg tttttccgcc atacggcggt caaggtgcaa
      481 ttccatattg ccggtgcctt tgaattcttc gtaaatcaca tcgtccatac ggctgccggt
      541 ttcaaccaat gcggtggcga tgatggtcag cgaaccgcct tcttccacgt tgcgcgccgc
      601 gccgaagaaa cgtttgggac gatgcagcgc gttggcatcg acaccgccgg tcaggatttt
      661 gcccgaggta ggcacgacgg tattgtaggc gcgggcaagg cgggtaatcg aatccagcag
      721 gatgaccacg tcttttttgt gttccaccat acgcttggct ttttcaagca ccatttcggc
      781 aacttggacg tggcgttgag ccggctcgtc aaaggtggag gagactactt cgccacggac
      841 ggagcggctc atttcggtta cttcttcggg acgttcgtca atcaagagga cgatgagttc
      901 gacttcggga tagtttgcgg taacggcgtg ggcaatgttt tgcagcatca cggttttacc
      961 gcttttgggc ggggcaacca agagggcgcg ctgacctttg ccgatagggg aaatcaggtc
     1021 gatggcacgt ccggtcaggt tttcttcgga ctttaagtcg cgttccagct tcaactgttc
     1081 ggtcggaaac agcggggtca ggttttcaaa caggatttta tggcggcata cttccgggtg
     1141 gtcgccgttg atggtatcaa gcctgaccag ggcaaaatag cgttcgttgt cttttgggac
     1201 gcgcacgctt ccttcgatgg tgtcgcccgt atgcaggttg aagcggcgga tttgggtggg
     1261 cgagacatag atgtcgtcgg ggccggcaag ataggacgtg tccgcgctgc ggaggaagcc
     1321 gaagccgtcg ggcaggattt caagcgtgcc ggagcaggtg aaaccctcgc cttttttcat
     1381 catctggcgg acgatggcaa atacgaggtc ttgtttgcgg aatcggttgg cgttttcgat
     1441 gccgtgttct tccgccaatt ctaagagttt ggaaatgtgc agggtttgta attcggagac
     1501 gtgcataata atgatgtatt ttgaagagga aaaagacagg cagatgccgt ctgaaagaag
     1561 aagctgaccg ttgccggttg ctcggggaag ggggaattgt aggcagtcgg cgcgtgggtg
     1621 tcaaatatta tcgcggacgg ggcatcggca ggaaatgccg tctgagcgga gctgcttgga
     1681 aaaaaatacc cccgcgcttt tcaggctcgg gggtatgggc attgattatt tgttcaattc
     1741 attcgccaaa tatagccaag tttcgatgac ggtatccggg ttcagggaaa cgctttcaat
     1801 gccttcctca accagccatt tggcgaagtc cggatggtcg gacgggcctt gaccgcagat
     1861 gccgacatat ttgttctgct tgcggcaggc ggagatggca aggtgcagca tcactttgac
     1921 ggcagggttg cgttcgtcaa acgattcgga taccaagccg ctgtcgcggt cgagaccgag
     1981 ggtcagttgg gtcatgtcgt tcgagccgat ggagaagccg tcgaagtatt gcaggaattg
     2041 ttccgccaat accgcgttgc tcggcagctc gcacatcata atcaggcgca ggccgttttt
     2101 gccgcgttcc aagccgtttt ctttcagggc tttgacaacg gcttcggctt cgcccaaagt
     2161 gcggacgaac ggaatcatga tttcaacgtt ggtcaacccc atttcatcgc ggacgcgttt
     2221 caaggctttg cattccaagg cgaaacagtc tttgaagttg tcggcgacat aacgcgccgc
     2281 accacggaag cccaacatcg ggttttcttc atgcggttcg tatacgttgc cgccgaccag
     2341 gttggcgtat tcgttggatt tgaagtcgga catacggacg atggttttac gcggataaac
     2401 cgatgcggcc aatgtcgcca cgccttcggc gattttatcg acgtagaagt cgacagggga
     2461 cgcgtaaccg gcgatacggc gggtaatttc cgcttttaat tcgtcgtctt gtttgtcaaa
     2521 ttccaacaag gctttggggt ggataccgat ttggcggttg atgataaatt ccatacgcgc
     2581 caagccgatg ccttcgctgg gcaggttggc gaagctgaat gcgagttcgg gattgccgac
     2641 gttcatcatg acttttacag gtgctttagg catattgtct aaggcgacat cggtaatctg
     2701 tacgtccaac agaccggcat agataaagcc ggtatcgcct tcggcacagg atacggtaac
     2761 ttcttgaccg tttttcagca attcggttgc attgccgcag ccgacaacgg caggaatgcc
     2821 caattcacgc gcgatgatgg cggcgtggca ggtacggccg ccgcggttgg taacgatggc
     2881 agaagcacgt ttcatcacgg gttcccaatc cggatcggtc atgtcggtaa cgagtacgtc
     2941 gccggcttcg acggaatcca tctcggaagc atctttaatc aggcgcacct tgccctgacc
     3001 gactttctga ccgatggcgc ggccttcgca taatacggtt ttgtcgccgt tgatggcgaa
     3061 gcggcgcagg ttgcggttgc cctcttcttg ggattttacg gtttcgggac gggcttgcag
     3121 gatgtagagt ttgccgtcca agccgtcgcg tccccattcg atatccatcg ggcggccgta
     3181 gtgtttttcg atggtcagtg cgtaatgcgc caactcagta atttcttcgt cggtaatgga
     3241 gaagcggttg cggtcttcct cggggacatc gacgttggtt acggatttac cggcttctgc
     3301 tttgtcggta aaaatcattt tgatgtgttt tgaacccatg gttttacgca ggatggcggg
     3361 cttgcccgct ttgagcgtgg gtttgaacac ataaaattcg tccgggttga ccgcaccttg
     3421 tacgacgttt tcgcccagac cgtaagagga ggtaacaaag acgacttgat cgtagccgga
     3481 ttcggtgtcg agggtgaaca tcacacctga tgcgccgctg tcggaacgca ccatgcgttg
     3541 aacgccggcg gaaagggcga cgatgtcgtg ttcgaagcct ttgtggacac ggtaagaaat
     3601 ggcacggtcg ttatacaggg aagcgaatac atggtgcatc gcttctttaa cgttatccaa
     3661 gccgttgatg ttcaagaagg tttcctgttg tccagcgaat gatgcgtccg gcaggtcttc
     3721 ggcagttgcg gaagaacgta cggcaacgga aatgtccgca ccgccggcat cggcaaccat
     3781 tttgttccat gccgcttcga tttcggcatc gagctgttcg gggaaaggcg tatccaaaat
     3841 ccattggcgg atttctttgc cgacgcgtgc cagttcggca acgtcttcga catccaattt
     3901 tgccagtgcg gcggaaatgc gttcgctcag accgttgtgt gcgaggaatg cgcggtaggc
     3961 ttcggccgtg gtggcaaagc cgccggggac gcgaacgcct ttttcggtca gctgactgat
     4021 catttcgccc agcgaggcgt ttttaccgcc cacgcgttca acatctgtca tacgcaggtt
     4081 ttcaaaccag attacgtagt tgtcggccat ttgtgtgtcc aatccaaaat atgttaaaaa
     4141 agaaacaaat ccgcgtgctt attttaagcg attcgttcct ctgctgtcat gtgttttatc
     4201 cgttttaaaa tcatgatgcc gtctgaaaaa ttgcggtttc ggcgtgtgta gcggtttgaa
     4261 acttacagcc ggtatacttc tttttttggg tattttcttt gtaaaacagg tggtttgaat
     4321 aggttaatgt tttttctgtt tgattttttt gtttattttt taaaattttc tgccaaaaaa
     4381 tactttatat aaataatttt atttcaaaat tatattgtgt ctgtttgggt gtaatccgag
     4441 gtaggtgtgc tgcgggtgct ttccttgtgt ctgctgctgc tgttatgatg ggattttaaa
     4501 cctgtgtttt aaggatggaa gatgagcagt ccgcgccatg tgttttacat ttccgaccgt
     4561 accggtctga ctgctgagaa tatcggcgag gcgttgctga accagtttgg caatctgtcg
     4621 ttcaaacgcc atacgcatcc gtttgtcgat acgccggaaa aggcgcgcgc ggtggtggag
     4681 aaggtcaatc ggagccggca ggaaaacggt cagcgtccga ttgcgtttgt cagtgtggtt
     4741 gatgacgaaa tccgtcggat tatcaaaggg gcggatgctt ttcagattaa tttctttgag
     4801 acttttttgg gactgttgga gaaggaactc aataccgaag ccacggcatc cgggcagggg
     4861 catcacagta tcggtaatac gaagcgttat gatgcgcgta tggaagcggt caatttttct
     4921 ttgaaccacg acgacggggt cagcgataag aaccttcagg aagcggatgt aatcttgatg
     4981 ggtgtatcgc gttcgggcaa aacgccgacc tgcctttacc tcgccctgca atacggcatc
     5041 cgtgcggcaa actatccgct gattcccgac gatttggaat cggccgatct gccgcgtatg
     5101 gtcaagcctt atagggataa gctgttcggg ttgaccatcc agccggaacg tttgcaggcc
     5161 atccgccaag agcgccgccc gaattcaact tatgccaaaa tcgatacatg ccgcagcgag
     5221 gtggcggacg cgcagagtat gttcagacgg catgggattc cgtttgcgaa tacgacggat
     5281 aagtcggttg aggaattggc ggtacacatc cttcaggcgt gcaagctcaa acgcaggttt
     5341 tgacgggctt tgattcggtt tgaaggcgga actgccgtct gaaatcaggt ttcagacggc
     5401 agt
//
LOCUS       DAWGRZ010000144         5330 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000144 DAWGRZ010000000
VERSION     DAWGRZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.144"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            304..1737
                     /locus_tag="WHT44_001674"
     CDS             304..1737
                     /locus_tag="WHT44_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ2533828.1"
                     /translation="MNEYSQLIKHPDISLPPVSDGIKVDNPATGETLAFVRKTDSDKL
                     KNLIQKAAAAQKLWAAKTALERADVLWRWYFQIKENKEALARLMTMEQGKSLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALASLAYEAGIPQDLLPVVSGSASEIGHEFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSAIYDEFCRKLSEKAAALKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALAKGASCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            1846..2634
                     /locus_tag="WHT44_001675"
     CDS             1846..2634
                     /locus_tag="WHT44_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ2533829.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDQWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            3002..4558
                     /locus_tag="WHT44_001676"
     CDS             3002..4558
                     /locus_tag="WHT44_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ2533830.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADSQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            4575..5321
                     /gene="surE"
                     /locus_tag="WHT44_001677"
     CDS             4575..5321
                     /gene="surE"
                     /locus_tag="WHT44_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ2533831.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEQALWTLLAHFFKNPPQSPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHAD"
BASE COUNT         1244 a         1357 c         1520 g         1209 t
ORIGIN      
        1 gttcggattt ttgccatact ctaccacacg ttgcaactgc aatctttgct ccttatatat
       61 tgcaccccat caaagggctg cattactttt ctttctaaag attactatcg tataaaaaat
      121 atttttctta aacgacaggg attgcgcccg cgccatattc agacaccgcc cggatgttgc
      181 gctgcccgat cggatgcttc agacggcatc ggaagggttt gcagttttgg aatatgaaga
      241 tgataatgtc cgcgagattg acggcatttg aaagcggtta ttccgcatac cggaggatac
      301 gaaatgaacg aatattccca attaatcaag catcccgata tttcccttcc cccggtttca
      361 gacggcatca aagtcgataa tccggcaacg ggcgagactt tggcgtttgt ccgcaagacg
      421 gattcggaca agctgaaaaa cctgattcaa aaagcagctg cagcacaaaa attatgggcg
      481 gcaaaaactg cgttggaacg cgccgatgtg ttgtggcgtt ggtattttca gattaaagaa
      541 aacaaagaag cattagcgcg cctgatgacg atggagcagg gcaaaagcct gaccgaggcg
      601 cgtggcgaaa tcgattatgc ggcttcgttt gtgcgctggt ttgccgaaga ggcgcggcgg
      661 attgacggcg atgtgctgac gagtgtgaaa gcgtcgcaaa aactggtcgt gttgaaacag
      721 cccgtcggcg ttaccgctgc gattacgccg tggaactttc cgtccgcgat gattgcgcgc
      781 aaggccgcgc ctgctttggc ggtgggttgc gcgatgatcg tcaaacccgc atcgctcacg
      841 cctttgagtg cgtatgcctt ggcttcgttg gcttacgaag cgggcatacc gcaggatttg
      901 ttgcctgttg tcagcggcag tgcttcggaa atcggccatg aatttgctac gaacccgatt
      961 atccgcaaaa tcagcttcac cggctcgacc gaagtcggcg caaaaatttt tgccgacagc
     1021 gcggcggaca ttaaaaaact cagtttggag ctgggcggca acgcgccgtt tatcgtgttt
     1081 gacgatgccg atttggacaa agccgtcgaa ggcgcgctcg ccagcaagtt ccgtaacagc
     1141 ggtcagacct gcgtctgcac caaccgcgtt tacgctcaat ccgccattta cgacgaattt
     1201 tgccgcaaat tgagtgaaaa agcagccgcg ctcaaattgg gcaacggctt ggaggatggt
     1261 gtgaaccaag ggccgctgat tgaggaaaaa gcggtggaga aagtcgagca gcacatcgcc
     1321 gacgcgcttg ctaaaggtgc aagctgcctg accggcggca aacgcagcgc gttgggcgga
     1381 acgtttttcg aaccgactgt tttaagcggc gtaacggcgc aaatggcggt ggcacgcgaa
     1441 gaaaccttcg ggccgttgtg tccggtattc cgttttgaaa ccgaagccga ggtcatcgag
     1501 gctgcgaaca atacggaata cggtttggca gcttaccttt tcaccgccga caccgcccgc
     1561 caatggcgcg tcggcgaagc cttggaatac ggtatggtcg gcatcaatac gggcttaatc
     1621 agcaatgaag cggcaccgtt cggcggcgtg aaacgtagcg gtttgggacg tgaaggcagc
     1681 aaatacggtg cggacgaata tctagaattg aaatatctgt gtatggatgt cgggtgacgg
     1741 atgccgtctg aacggcagga tttcagacgg cgccgcattt taagcagtct ctatctgttg
     1801 tacaatgcgc cctgttttta cggttatttt tgatttgaac aagatatgat ggaaaacgga
     1861 aaaacagtcc cgagatggcg ttttgccttg aaaagtgcgg gctggcacct cttaatcagc
     1921 ctgtcggttg cagggctggc ggcattgctg gtttttaagg tttggtatcc ttatccttat
     1981 gccgagctga cgggagggct gtcgctttat cagctggtgg tggctgtcga tattgtatgt
     2041 ggtccgctgc tgactttaat tttggcaagc ccgaagaaaa agacaaaggc acgcatggtc
     2101 gatttttcca tggtcggcat catccagctg gcggctttgg tgtacggtct gcacagcgtt
     2161 tcgctggcgc gtcctgtggt ggaagcgttt gaacaggatc gtatgaccat tgttacggcg
     2221 gcggaagtcg tggtcgaaga tttgcacaaa gcccccgaag ggctgcaaag cctgtcgtgg
     2281 ttcggcatcc gccgcattgc attgaaagaa cctgaggatg cggatgagaa gaacaagacg
     2341 ctggatttgt ccctgaaagg tatcgagccg agtatgcgtc ccgaccagtg gctgccgtat
     2401 tccgacaagg aagcagaaga aatccgcaaa catctgaaac cgctgaaagt cttggcggat
     2461 gcgagaaaaa cgacggttgc ggacattctg aaacaggcag gtctcgccga aggggaggag
     2521 ctgtattacc tgccgtttac cagcagcagg cagaaagagt ggatagtcat taccgataaa
     2581 gagggcaaca ccaaaggcta cgcgccgata gacggcttca tcatcacccc ttaagcgttg
     2641 ggactccgtc cgcactcgaa catccgttct tcgcggcggt agaatcagac tgtatttgag
     2701 aggggcgcaa cccctcaatc agggcaacac ataggggaag gctttatgtg ccgtccgtgt
     2761 gttgaaacat ttcaaaataa gctcatgccg tccgaacatc ctttcagacg gtatggcgtt
     2821 ttgccattgc cccgataagc tgttaaacta tttaaattat ttcgaccgaa ggtacacgca
     2881 catgcaagac cccgagcaga gcagcaagcc cgcccgccgt tttttgtgcg taccgtctgt
     2941 tcaggcagct tgagcgcttc aggctgcctt ttcgcgtttg aacttgagga atacgaaaat
     3001 catggatttc agttggttgg cagaaccgca tacctggata ggttttgcca cgcttttggt
     3061 gttggaagtc gtattgggga tagacaatct tgtctttgtg gcgattttgg caaacaaggt
     3121 ccagcccgca cggcgcgacc gcgcacggat tatcgggctg gggctggcag tcgtcatccg
     3181 catcattatg cttgctttta tggcgcacat catcacgctg accgagccgc tgttccaaat
     3241 cggcggcctc gccgtttccg gcaaggacat gattatgctc gcgggcggta ttttcctgct
     3301 ttacaaagcc accaccgaac tgcatgaacg cctcgaaggg cacaaccgtt ttaccgttgc
     3361 cgacagccaa aaaaaacacg cgccgttttg gggcgtggtc gcgcaaatcc tgatactgga
     3421 tgccgtgttt tccatcgatt cggtcattac tgcggtggcg atggtcgatc atatcgtcgt
     3481 ggcgatgggt gcggtcgtcg tcgcgatggc tgtaatgatt tctgccagca aactcttgac
     3541 cgaatttgtc gacagacacc ctaccgtcgt gatgctctgc cttggttttt tgttgatgat
     3601 cggtttcagc ctgattgccg aagccttcca tttccacatt cccaaaggct acctctacgc
     3661 cgccatcggc ttctcgattt taatcgaatt gtttaaccag atttcgcagc gcaacagccg
     3721 caaaaacgac tacatcggca gctcgtggcg caagcgcacc gccgaaaacg tcttgggtat
     3781 gatgggtata cgcgaaagcg tgcttgccga cgcgggcggc gaatccgggg acgacgcgca
     3841 ttttgaagaa aacgaaaaat cgatgatacg cagcgtgctg acgcttgccg aacgcccgat
     3901 tatgggggtg atgatcccac gccgcgacat cgaacggctg gacatttccc aaagccgcga
     3961 agaacagtgt gcccaactgc aaaacacgcc ttacagccgc ctgctcgttg tcggtaaggc
     4021 gggcgtggac gaacctttgg gctacatcaa caaaaaagac ctgctgtccc aactgctgga
     4081 aacaggcggt ctcgacattc agacggcatt gcgccagccg ctcgtcctgc ccgacagcac
     4141 caccgcgctg ggcgcaatcg aactcttccg ccaaagcagc gcggattatg ctttggtggt
     4201 ggacgagttc ggcgcggtat tgggcatggt aaccatgaaa gacctgctcg aaaccatcgc
     4261 aggcgagttc cccgaagaat ttgagcgcga agaagaacca gccgttcagg ggaatcccga
     4321 tgaaagcctg acggtggaag gcgcgttgga atatgtggaa ctcgcaccgc aactcaacct
     4381 gccgcagcag gaggaagatg ccgatttcca tacggttgcc gggctgatta tggaagaatt
     4441 gcaaaccatc cccgatgtcg gcgattttgc cgatttccac ggctggcggt ttgaagtggt
     4501 cgaaaaagaa gggcagcgca tcgagcgggt caaaatcacc aaattgcccg aagaataagc
     4561 attcaggata gaaaatgaac gttttgattt ccaacgacga cggctacctc tccgaaggca
     4621 ttgccgtttt ggcgcgcgtt acggcggaat ttgccaacgt cagggtggtc gcgcccgaac
     4681 gcgacaggag cggggtcagc aattcgctga cgctggaacg ccctttgcag ttgaaacagg
     4741 cgcaaaacgg gttctactat gtcaacggca cgccgaccga ctgcatccac atcgggcagt
     4801 ctgtattttc ggattttcag gccgattttg tcttttcggg catcaaccgg ggcgcgaata
     4861 tgggggacga cacgctttat tcggggacgg ttgcggcggc aaccgaagcc taccttatgg
     4921 gcatacccgc cgtggcgttt tccttaaacg acgcttccgg acgctattgg gcgaccgcag
     4981 aacaggcact gtggacattg ttggcgcatt ttttcaaaaa ccccccgcag tcccctattt
     5041 tgtggaacat caatatcccc gccgttgcgc cggaagatgt gcggggcatt aaaatcgccc
     5101 gtttgggcag gaggcatcac ggtcagaacg tcattcccgc gcgcaatccg cgcggcgaac
     5161 agatttattg gataggaccg gtcggcgaag tttccgatcg ggaagaggga acggatttcg
     5221 gtgaatgcgg cgcaggtttc attaccgtaa cgccgctgca aatcgacctg accgcctatc
     5281 cggacatggc ggaaacagcg gcgttctggc atgcggactg accgtttcat
//
LOCUS       DAWGRZ010000145         5255 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000145 DAWGRZ010000000
VERSION     DAWGRZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.145"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            109..804
                     /gene="pgl"
                     /locus_tag="WHT44_001678"
     CDS             109..804
                     /gene="pgl"
                     /locus_tag="WHT44_001678"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ2533832.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            785..1771
                     /locus_tag="WHT44_001679"
     CDS             785..1771
                     /locus_tag="WHT44_001679"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ2533833.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGTSPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1821..2669
                     /gene="hexR"
                     /locus_tag="WHT44_001680"
     CDS             1821..2669
                     /gene="hexR"
                     /locus_tag="WHT44_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ2533834.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            2790..4436
                     /gene="pgi"
                     /locus_tag="WHT44_001681"
     CDS             2790..4436
                     /gene="pgi"
                     /locus_tag="WHT44_001681"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ2533835.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEV
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            4450..5001
                     /locus_tag="WHT44_001682"
     CDS             4450..5001
                     /locus_tag="WHT44_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ2533836.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKVKQRNEELEAAEEAAAQEALGREQEAARVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGKTPNGIKFSELACKTAKTEARLEELH
                     NRKKALIDEMAREADKKELSKRL"
BASE COUNT         1337 a         1460 c         1389 g         1069 t
ORIGIN      
        1 cagtcccgat atttggtatc aaaagcataa acaactgttc gaccggcata ttgctcaacc
       61 cgtttccata acggaatatg ccgtctgaaa taaaaaaagg acacaaatat gtttgtttgg
      121 cacgaatacg aaaatgcggc agaagcggcg cagtctttgg ctgacgcagt ggcggatgct
      181 ttgcagggcg cactggacga gaagggcggt gcggtgttgg cagtttccgg cggacgttcg
      241 ccgattgcat ttttcaacgc cctgtcgcaa aaagatttgg attggaaaaa cgtcggcatc
      301 accttggcag atgaacgcat cgtgccgacc gtccacgccg acagcaatac cggtttggtg
      361 cgcgaatacc tgttgaagaa caaagcggaa gcggcaatgt ggattcctat ggtggaagac
      421 ggaaaaactg aaaccgaatt acatcccgat gctgttgtcg attatgcact gaaacattac
      481 aaacagcccg atgttttggt tttgggtatg ggaaacgacg ggcatacggc ttcgattttc
      541 ccgaaagctc cacagtttca gacggcaatc gacggttcgg caggtgtcgc gctggtgcat
      601 accacgcccg ttaccgcgcc gcacgagcgc gtcagtatga ccttggatgc gattgcccat
      661 acggggcatg tgtttttggc gatacggggc gaagagaaaa aagccgtgtt cgaccaagcc
      721 gcacaaggcg aaaaccgcga atatccgatc aacctcgttt tgaaccatca aggagtgaac
      781 tgccatgtct tctacgccga ataaacaagc cggatatccc cgactggtcg ccgatatcgg
      841 cgggacgaat gcacgctttg cgctggaaac cgcgccgcgc gtcattgaaa aagccgccgt
      901 gcttccgtgt aaagactacg atacggttac cgatgcggtg cgtgcctatc tgaatcaaag
      961 cggtgcaaca gccgtacggc acgcggcatt tgccatcgcc aacccgattt tgggcgactg
     1021 ggtgcagatg accaaccacc attgggcgtt ttccatcgaa accacccgtc agactttggg
     1081 gctggacacc ctcatccttt tgaacgactt taccgcgcag gcattggcgg taacgcagac
     1141 ttcaagcaaa gacctgatgc aggtaggcgg gcaaaagcct gtcgaatttg cccccaaagc
     1201 cgttatcggc cccggtaccg gcctgggcgt gagcggattg gtgcacagcc acgcaggctg
     1261 ggtggctttg gcgggcgagg gagggcatac cagtttcccg ccgtttgacg atatggaagt
     1321 gctgatttgg cagtacgcca aaaacaaata cggccatgtt tccgccgaac gctttttgag
     1381 cggcgcgggc ttgagcttgg tttacgaggc tttggctgca aaacagaaag ccaaacccgc
     1441 caaactgatg ccgtctgaaa tcacggaaaa ggctttgagc ggcacgtcgc ctttgtgccg
     1501 tcagactttg gacatcttct gcgccatgct cggcacggtt gcttccaacc tcgccctgac
     1561 gctgggcgcg cgcggcggcg tgtacctgtg cggcggcatt attccccgcg tgttggaata
     1621 cttcaaaact tccccgttcc gcagccgttt cgagaacaag ggcaggtttg aagcatatct
     1681 tgccgcgatt cccgtgtatg tcgtcttgag cgagtttccc ggaatttccg gtgcggctgc
     1741 ggctcttgac aaccatttga gaaacgttta accacagcgg ctccttgcag cggggctgca
     1801 ttatcgaagg gcatatcatt atgttaagca aaatcagcga atcactggca aacctttccg
     1861 gtgcggaacg caaagtcgcc gaatgtgcat tggcggaacc caaatggttc gtccatgccg
     1921 ccgttgccga aattgccgaa cgtgcgtccg tcagccagcc gaccgtcatc cgattctgcc
     1981 gcagcttggg ttataaaggg ctgcccgagt tcaagctcgc cttgtccgcc agcatcggtc
     2041 atgagggtat gccctatgtc cacgaagaac tcaacgccga cgacgatatg gcaagcgtgg
     2101 tcgagaaagt gttgggcaat gccgccgcct cgctgttggg cgaacgccgc ttcctgaaag
     2161 agtcggagct ggaaaacgcc attgccacgc tgatgcacgc ccgtcgcgtc gagttttacg
     2221 gtgtcggcaa ttccggcatt gtggcacagg acgcgcagca taaatttttc cgtttcggca
     2281 tgtccacggt cgcctatgtc gatacgcaca cgcagctgat ggcggcatct gttttgagcg
     2341 atcaggatgt tttggttgcc atttccaaca cgggttcgtc tatcgaactt ttggatgcgg
     2401 tcagcatcgc caaagaaaac ggcgcgtctg tcatcgcact gacccgcaac gattcgcctc
     2461 tggcgcaact tgccgactgc gtgttgagcg ttgccacaca ggaaaatgcc gaactctaca
     2521 cgcccatggt ttcccgcctc ttgcagcttg ccgtcatcga cattctcgcc atcggacttg
     2581 ccctgcgctt gggcgatgct gccagcctgc aattgcagaa aagcaaaaaa agcatacaca
     2641 acaagcacat cgattacgac aaagattgac cttcagacgg catcccacaa atgccgtctg
     2701 aaatgccgaa caacggtcgt cggcggcttg cggcagtttc cggcagcctt ttcatcccac
     2761 aacaaaaaac ctcattcagg agcatataga tgaaacacct tcacgactta cccgcatggt
     2821 cgaaattgtg gaatcacttt gacgacagca aaacattgca tatgcgcgaa atgttcgagc
     2881 aagacccgca gcgtgcggaa cgctactggc tgcaagtcgg cggactgacg ctggactact
     2941 ccaaaaaccg catcaacgac gaaaccatgt cgcttttgtt cgagcttgcc cgagaagcag
     3001 gcgtgccgga gcggatgcgg cagatgttcc acggcgaaaa aatcaatacc accgaaaacc
     3061 gcgccgtcct gcatgtcgcc cttcgcaacc gcaccaattc gccgattgtg gttgacggtg
     3121 aagatgtgat gcccaaagtc aaccgcgttt tgcaacgtat gggcgaattt gcacacgaag
     3181 tccgcagcgg aagctggctg ggctatacca accaagtcat taccgacgtt gtcaacatcg
     3241 gcatcggcgg atcggatttg ggtccgctga tgatgtgtac cgcgctcaaa cctttcggtc
     3301 atccgcgcct caatatgcac ttcgtctcca acgtggacgg ctcgcaactg cgcgacgtat
     3361 tgtccaaagt ccaccccgaa accacgttgt tcatcatcgc ctccaaaaca tttaccacgc
     3421 aggaaacgct gaccaacgcg ctgaccgcgc gcgaatggtt tttgaatcat gcgggcgacg
     3481 aagaagccgt tgccaaacac ttcgccgccg tttccaccaa tcaaaaagcc gtcgccgaat
     3541 tcggcatcga caccgccaat atgtttgaat tttgggattg ggtcggcggt cggtacagcc
     3601 tgtggtccgc catcggattg ccgattatgc tgtatctcgg cgaagaaaac ttcattgaaa
     3661 tgctcaacgg cgcgcacctg atggaccaac acttcatcaa cacaccgctc gagcgcaacc
     3721 tgcccgtcat tctcgccctc atcggcatct ggtatatcaa ctactacggc ggcggcagcc
     3781 acgtcatcgc gccttacgac caacatttgc accgcctgcc caaattcatc cagcagctcg
     3841 atatggaaag taacggcaaa caggttacgt tggacggcaa agcagtcgga cacgaaacct
     3901 cgccgattat ctggggcgaa acgggcatta acggccagca cgcctttttc caactgctgc
     3961 accaaggcac gcacattacc cccatcgacc tgattgcctc gcttgaaaaa cgcagcaacc
     4021 tgcccggaca ccacgaaatc ctgcttgcca acgtcttcgc ccaagcagaa gcctttatgc
     4081 gcggcaaaac ccccgacgaa gtccgcgccg aactcaaagc gcagggtatg gatgaggtgc
     4141 gcatcgaaga gctggtcccg cacaaaacct tctccggcaa ccgcccgacc aacctcattc
     4201 tcatggacaa ggtcaacccg cgcaatatgg gcagcctgat tgccatgtac gaacacaaaa
     4261 ccttcgtaca aggcatcatt tggggcatca acagcttcga ccagtggggc gtggaactcg
     4321 gcaaacaact ggctaaaacc attttgggcg aactgaccgg cgaaaccggg ccgcaaaagc
     4381 acgacagttc gaccgaacgc ctgatcaacc tctacctgca gaccaaccgc aaataaaacc
     4441 tgcggaaaaa tgccgtctga acgccgaccg tttcaaacgg catttttatc gaacaggaaa
     4501 accgtcggta aacttgcaga gcgtgtgcaa tcccgatatg atggtttgca taaatttaaa
     4561 catatatgtt ccgcagctat ggcactgatt aaagagccgt tggacaaagt gaaacaaagg
     4621 aacgaagaac ttgaagcggc agaagaagcg gcggcgcagg aggcattggg tcgggagcag
     4681 gaagccgccc gcgtatccga atgggaagaa cgctacaagc tgtcgcgcag cgagttcgag
     4741 cagttctgga aaggattgcc tcaaaccgta cagaataagc tgcaagcctc acagaaaaca
     4801 tggaaaagcg ggatggataa aatctgtgcc aacaatgcga aagctgaagg taaaacgcca
     4861 aacggcataa aattcagcga actggcatgc aaaacggcga aaaccgaagc acgcttggaa
     4921 gagctgcaca accgtaaaaa agcccttatc gacgaaatgg ccagggaagc ggacaagaaa
     4981 gaactgtcaa agcggctctg aacagcgcgg ttcaggcact gcccgccgat attgccgaaa
     5041 ccgtcatgcc cgagtaccgc aactgacaaa acggtttgaa tgccaaatgc gccgacggcg
     5101 acgaatacgg cgtggcacaa tctgactgcc gtaccagaga aatcaatgcg aaaaccaaag
     5161 aaatccaagg ttatctgatt gactgaaact tggatgcggg aatgtcggcg gcttttgcgt
     5221 ttttgtcgtt tttatagtga attaaattta aaccg
//
LOCUS       DAWGRZ010000146         5233 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000146 DAWGRZ010000000
VERSION     DAWGRZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.146"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            86..1561
                     /locus_tag="WHT44_001683"
     CDS             86..1561
                     /locus_tag="WHT44_001683"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ2533837.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIRAAQMVQEGLDK"
     gene            1558..2103
                     /locus_tag="WHT44_001684"
     CDS             1558..2103
                     /locus_tag="WHT44_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ2533838.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRSAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            2100..3236
                     /locus_tag="WHT44_001685"
     CDS             2100..3236
                     /locus_tag="WHT44_001685"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ2533839.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPNPNA
                     PAAEFADRHLCAPFDNQTALEELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLSTDSDTAFQEAKKLHQSL"
     gene            3283..3765
                     /locus_tag="WHT44_001686"
     CDS             3283..3765
                     /locus_tag="WHT44_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ2533840.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            3871..4926
                     /locus_tag="WHT44_001687"
     CDS             3871..4926
                     /locus_tag="WHT44_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ2533841.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEKKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            4923..5216
                     /locus_tag="WHT44_001688"
     CDS             4923..5216
                     /locus_tag="WHT44_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533842.1"
                     /translation="MTAMPSEPPFRRHFTHQNRKTAPKIIITKHNGKPDTKPNTAAKM
                     PSEAVGATILIWGPLQGRITFLNHPFDSQINSAFACQKQNQYFAVRIGTFIVD"
BASE COUNT         1497 a         1607 c         1106 g         1023 t
ORIGIN      
        1 cggttagact gtcaacaatt ccgataaaat gctatactcg cgcccattgc cgcgccgtcc
       61 gaaaccgccc tttgggaaag cagaaatgat cagcaaacaa gaataccaag cccaagccgc
      121 ccaaggctac aaccgcatcc cgctcgtgca agaactcctt gccgatttgg atacgccgct
      181 ttccctctat ctcaaactcg ccaaccgccc ctatacctac ctgctcgaat ccgttgtcgg
      241 tggcgaacgt ttcggccgct attcttttat cggcctgcct tgcagccact atctcaaagc
      301 cagcggcaaa cacgtcgatg tttatcaaaa cggcgaaatt gtcgagcaac acgacggcaa
      361 ccccctgccc tttatcgaag ccttccacaa ccgcttcaaa acacccgaaa tcccaagcct
      421 gccgcgcttt accggcggac tggtcggcta cttcggttac gaaaccatct acaattttga
      481 acacttcgcc caccgcctga aaaacaccac caaagccgac ccgctcggca cgcccgacat
      541 cttgctgatg ctgtcgcaag aattggcggt gatcgacaat ttgagcggca aaatccatct
      601 cgtcgtttac gccgatccgt cgcagcccga cggctacgaa cgcgcccgcg aacgcctcga
      661 agacatccgc acccagttgc gccaaagctg cgccatcccg ctctcgctcg gcagcaaaca
      721 caccgaagcc gtcagcgaat tcggcgaaga gccgttcaaa gcctgcgtta acaaaatcaa
      781 agactacatc ttcgcaggcg actgcatgca ggttgtcccc agccagcgca tgagcatgga
      841 attcaccgac agcccgctcg ccctctaccg cgccctgcgt acgctcaacc cttcgcctta
      901 tctcttttac tacgatttcg gcgatttcca catcgtcggc tcctcgcccg aaatcctcgt
      961 ccgccgcgaa cgcgacgatg tcatcgtccg ccccatcgcc ggcacgcgcc tgcgcggcaa
     1021 aacccccgcc gaagaccttg ccaacgaaca agatttgtta agcgatgcca aagaaatcgc
     1081 cgaacacgtc atgttgattg acttaggacg caacgacgtc ggccgcatca gtaaaaccgg
     1141 cgaagtcaaa gttaccgaca aaatggtgat tgaaaaatac tcccatgtaa tgcatatcgt
     1201 ttccaacgtc gaaggccgtc tgaaagacgg catgaccaac atggacatcc tcgccgctac
     1261 cttccctgcc ggcacactct ccggcgcacc caaagtccgc gccatggaaa tcatcgaaga
     1321 agtcgaaccg agcaaacgcg gcatctacgg cggtgccgtc ggcgtatggg gtttcaacaa
     1381 cgacatggat ttggcaatcg ccatccgcac cgccgtagta aaaaacaaca cgctatacgt
     1441 ccaaagcggc gcgggcgtgg ttgccgactc cgatccggcc tccgaatggc aggaaacaca
     1501 aaacaaagcc cgagccgtta tacgcgccgc gcagatggtg caggaagggt tggataaatg
     1561 acttatgaaa aactttatga gcttcgccaa acactcagac cgactaccaa agacggtctc
     1621 tatacagata atgaaaaaag tcgagaaatc ctaacggtta gatggagcgg caacacagaa
     1681 aatcctaaaa atttctcagc tgttgctata ggaattaatc caagtaaagc aaatgatgaa
     1741 agaagcgaca aaacccttac ccaacttgca cgtttcttgg atatgtacgg ttttaccaac
     1801 ttcaaaatgt taaatatttt ttccagttac tctacacagc aaacaggaat tagggcaaac
     1861 acccaaaccg atttttccaa atttaaagga tgtttggaag atgctgatat gattattctt
     1921 gcatggggta cagaccgcag tgcttataag gatgaaaaaa acagaatact ggaatttcta
     1981 aaagcagaaa aatttatgga aaaagtattc tgtatttctg aaactggtaa ttccagcgat
     2041 acaagacatc cgtcaagaat tagttatagc tatcaacttg tacaatttga agagtcagca
     2101 tgaaaaacat atctctttct ccgcccgcca tgctcggcat tcttggcggc ggacaattag
     2161 gcagaatgtt tactgttgct gccaaaacca tgggctacaa agtaaccgta ctcgatccca
     2221 acccgaatgc gccggcagcg gaatttgccg accgccattt gtgtgcgccg tttgacaacc
     2281 aaaccgcttt ggaagaattg gcaaaatgtg cggctgttac gaccgagttc gaaaacgtca
     2341 atgccgatgc gatgcgtttt ctcgccaaac ataccaatgt ttcccccagc ggcgactgcg
     2401 ttgccatcgc gcaaaaccgc attcaggaaa aggcatggat acgcaaagca ggcctgcaaa
     2461 ccgcgccgta tcaagcaatt tgcaaagccg aagacatcac tgaagaaagc atacaatttc
     2521 tgcccggcat cctgaaaacc gctacattgg gctatgacgg caaaggccaa atccgcgtca
     2581 aaacggtgga tgaactcaaa gccgcgtttg ccgaacaccg cggcgtggat tgcgttttgg
     2641 aaaaaatggt ggacttgcgc ggcgaaattt ccgttatcgt atgccgtctg aacaatgaca
     2701 acgtgcaaac tttcgatcct gccgaaaaca ttcacgaaaa cggtatcctc gcctactcca
     2761 tcgtcccagc ccgactgagt gccgacattc agcaacaggc gcgacaaatg gcgcagcgtt
     2821 tggccgatga attgaactac gtcggcgtat tggcggtaga aatgtttgtt gtcggcgaca
     2881 cgcatgaatt ggtcgtcaac gaaatcgcgc cgcgtccgca caattccggc caccataccg
     2941 tcgacgcctg cgcggcagac caattccagc aacaggtccg cctgatgtgc aacctgccac
     3001 ctgctgacac caaattgctg agttcttgct gtatggcgaa tattttgggc gacgtttggc
     3061 aggaagacgg cggcgaaccg gattggtttc ccctgcaaag ccggccggac gcgcacctgc
     3121 acctttacgg caaaaaaacc gcgcacaaag ggcggaaaat gggacacttt accgttttaa
     3181 gcaccgattc ggacaccgca tttcaagaag caaaaaaact gcatcagtcc ctataatccg
     3241 accaacggca cagatgccgt ctgaagccgc cgacatactg ccatgaacct gctgacactg
     3301 ttacgccccg ccaccgtgca agactgcaaa gacatcttca aagtccacct gcactccgtc
     3361 caatacacct gcatcctcag ctacaacgaa cacgccctaa aagtatggga aggcttgtta
     3421 aacacagaaa gctacctgcc gaccatttcc gatcccgaca aagcactttg ggttgccgaa
     3481 tacaaaggca acatacaggg atttttccaa atcgactgcc aagaagcaca actcgatgca
     3541 ctttacgtcc accccctgtt ccacaacctc ggactgggca ctgccctgct tcatcaggcg
     3601 gaaaccatcg cccacaaatc cggtttgagc tttctgaaac tctacgcatc gctcaactcc
     3661 gtcccgtttt accttctcaa ccgctacgaa tcgctcggtt cggcagtatt gcagcttgac
     3721 cccagcatca aaatcaaatg cgaactgatg cggaaacacc tctgatccgt ccttataacc
     3781 aaaactgata atcttcaaat caaacattat ttccacaagc gcagacagcc gtataaagtt
     3841 gccccatcta ccaaccgaca ggacaagcat atgaaaacct acgcaccggc actctatacc
     3901 gcagccctgc tcaccgcctg cagccccgca gccgattcaa accatccgtc cggacaaaat
     3961 gccccggcca ataccgaatc cgacggaaaa aacattaccc tgctcaacgc ctcatacgat
     4021 gtggcacggg atttttacaa agaatacaac cccttattta tcaaaacata ccaatccgaa
     4081 caccccggca catccgtcag catccaacag tcccacggcg gctccagcaa acaggcatta
     4141 tccgtagcca acggccttca agccgatgtc gtaaccatga accaatcctc cgacatcgac
     4201 ctgctcgaaa aaaaaggact ggtagaaaaa ggctggcaac aagccctccc cgaccacgcc
     4261 gcgccctaca ccagcactat ggttttcctt gtccgaaaaa acaaccccaa acagatccgc
     4321 gattggaacg accttgccaa agacggcgtt aacatcgtca tcgccaatcc caaaacctcg
     4381 ggcaacggac gctacgcctt cctcggcgca tacggttacg gtctgaaaac caccaacggc
     4441 aacgaacagg aagcccaaaa actcgtcgca tccatcctca aaaacacccc cgtttttgaa
     4501 aacggcggac gcgccgccac caccaccttc acacaacgca acatcggcga cgtactcatc
     4561 acttttgaaa acgaagccaa ctacgtcagc aaaaaactga cccaaggaca gtttgaaatc
     4621 gtctatccga gctacaccat ttccgccgaa agccccgtcg ccgtcgtcaa cagcgtcgtc
     4681 gcgaaaaaag gcacgcaaaa aaccgcccgc gcctatctcg aatacctttg gagcgaaccg
     4741 gcgcaagaac tcgccgcctc actctacctg cgcccccgca accccgaagt attggcaaga
     4801 cacaaagccg acttcccaga cttagacacc ttctctcccg aaaaaaaatt cggcggatgg
     4861 gacaacatca tgaaaaccta cttcgccgac ggcggcatat tcgaccgcct gaccgcacaa
     4921 aaatgaccgc tatgccgtct gaaccgcctt tcagacggca tttcacacat caaaacagaa
     4981 aaaccgcgcc caaaatcatc atcacaaaac acaacggcaa accggataca aaacccaaca
     5041 ccgccgccaa aatgccgtct gaagccgtcg gcgcaaccat ccttatctgg ggcccgcttc
     5101 aggggcgcat aactttcctt aaccatcctt ttgacagcca aataaacagt gcttttgcct
     5161 gtcagaaaca aaaccaatat tttgcagttc gtatcggaac atttatagtg gattaacaaa
     5221 aaccagtaca gcg
//
LOCUS       DAWGRZ010000147         5152 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000147 DAWGRZ010000000
VERSION     DAWGRZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.147"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(61..1317)
                     /locus_tag="WHT44_001689"
     CDS             complement(61..1317)
                     /locus_tag="WHT44_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ2533843.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFREHKKLGSQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETAFLG
                     NLKARKTEFSDSLNTAAELPQWLVEQLKQHWREEEILAFGRSINQPAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERIGSEHDARIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLAGLKIDL
                     DTGKYLRLNSARHQTDGFFAAVLQRK"
     gene            complement(1665..2006)
                     /locus_tag="WHT44_001690"
     CDS             complement(1665..2006)
                     /locus_tag="WHT44_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533844.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2210..3067)
                     /locus_tag="WHT44_001691"
     CDS             complement(2210..3067)
                     /locus_tag="WHT44_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ2533845.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELSVRELLGE
                     HGFRYLLALDADPTRLTDYLAQRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVARLNGKPYHIELTCKYYGGDTDSPEGMRGFDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLNVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3128..3943)
                     /locus_tag="WHT44_001692"
     CDS             complement(3128..3943)
                     /locus_tag="WHT44_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ2533846.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLIRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            4187..4972
                     /locus_tag="WHT44_001693"
     CDS             4187..4972
                     /locus_tag="WHT44_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ2533847.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDGWLESGDIVAANPKVLAQMLKIISAHV"
BASE COUNT         1171 a         1191 c         1408 g         1382 t
ORIGIN      
        1 tgatgaaatg ccgtctgaac gctttgaaaa gggttcagac ggcattttgt tcaaaccggt
       61 ttatttgcgt tgcaatacgg cggcgaagaa gccgtcggtt tggtgtcggg cggagttgag
      121 gcgcaggtat ttgccggtat ccaaatcgat tttcaaaccg gcaagcagtt cggcgcagtt
      181 gacgggttca aattcgggat gttcggacag gaaacgttcg acttgcagct cgttttcttc
      241 gggcaggatg ctgcaagtgg cgtacaccaa acgtccttgc ggttttacca gtttggaggc
      301 ggcatcgagg atgctgtgtt gctgttccaa aaggttggcg acggtttcgg cggattggcg
      361 gtatttgagg tcgggattgc ggcgtaaagt gcccaaaccg gagcagggcg cgtccaccaa
      421 cacacggtcg gctttgcctg ccagtcgggc gatacgggca tcgtgttcgc tgccgatgcg
      481 ttcggggtgg atattggtca gtccggcgcg ggtcatacgc ggtttgaggt tggcaaggcg
      541 tttttcggcg atatcgaagg cgtagattct gcctttgttc gccatttgcg caccgacagc
      601 caaggtttta ccgccggcac cggcacagaa atcgacaatg atttcgcctc gttttgcgcc
      661 caccaataag gcaagcagct ggctgccttc gtcttggact tccagtgtgc cgtctaaaaa
      721 cagttcgtgt ttgttaagcg cgattttgtt tttcaggcgg atgccccaag gcgaataagg
      781 cgttgcctct gcatcggcac tttcggcttg caacagcggc agcactttat cgcgtttgcc
      841 tttcaaagtg ttgacgcgga tgtcgagcgg ggcaggctgg ttgatgctgc ggccgaaagc
      901 gaggatttct tcttcgcgcc aatgctgttt cagttgttcc accagccatt gcggcaattc
      961 tgcggcggta ttcaggctgt ctgaaaactc ggttttacgg gctttcaaat tgccgaggaa
     1021 cgctgtttct tcttcatcaa gcaggtcttt gatttggctg atgttggtgc ttctgccgag
     1081 aaccagtgcg gcgagagcgg ctttgcgcgg ctgcgcgtgc ggacggcgta gggcggtact
     1141 gattttttga tagtggcgca gcgcggcaaa ggcggtttcg gcgatttcgt ggcgatcttg
     1201 actgccgagc tttttgtgtt cgcggaaata ggcggagagg acggcatcgg caggctgttt
     1261 gaaagtcagc atttcagcca aaactttggc ggtatggtcg agttgtgcgg cgttcataat
     1321 gtaatgggtt ctcttactgt gggttattca taatcaatct aacgtcctta aaataacaat
     1381 agcatcttta tcattactta gtattctatc cgcaataaga aagatgtgtt aaaagtgcgc
     1441 aatcgggatt tgtctaaatt tctccgttaa ctacaaaggc agcctgaaaa aagttctatt
     1501 ttcaggtcgc ttgaacagca tccgatataa tcacatcagc gatttcattt tgtaatcact
     1561 ccctaaaaca tgaggaaaaa aacaaaattt attttcttcg ttcagtatat ctatcctaaa
     1621 tatgccatca ctccattgag tttttaattt gtatttcgct cttgttaaat gtttttctgt
     1681 ttgccgattt cttttgctaa tgtaaaaaat actctaccac tctcattggt taaagttttc
     1741 aagtattcat caacatcagt aacattaata acaactgttt catttgtatg tttattaaat
     1801 tctaaatcaa taataatatt cccattaacc aatatttcat atatatcttc ttcttcaggg
     1861 agcatagaca aacaataagc acttgaagct gaaaactctt ttttcagata ctgaagaaga
     1921 aaagtatttt taattaaaat attattaaat ttatctaatg attgcttgac ttctttagtt
     1981 gtccaatttt taacattatt cttcattgta ttctacctgg ggtcgtagtt attaaattac
     2041 catacttatt tgtttggatc gtcataatat tagttggcat gttgttgtta tatttatcat
     2101 atccaatatc cttattaact cggatgactc tttcataaat tgtatgggaa atgagatttc
     2161 catcactaag cctctcaata gatatacgcg ttaatgttgg gaaagggttt cagacggcct
     2221 tcataatgcg ttcgatttcg tgccaaaagc cgtccggccg acattccaaa acggcaatca
     2281 agccttggtc gatacggcgg atttcggttg cgttcaatgt ttcggtttcg gcgacacgcg
     2341 caggcgcgag gtaggccata cggtcgagca gatggtagcg gacttcttgg cgtttaaacc
     2401 cgtattccgc ccaatcttga atatagatgc cgcgccaacc gtatggatta agcggtggct
     2461 caaaagcatg gaaaccgtgt ggaaaaaatc cgatgccgcg cacgatggaa acgggtttta
     2521 cgttaagcgg caaaccgtgc tgccgcaagg tccggatgcc gtctgaagtg tgcggcagac
     2581 cgagttgggc ggtcagtttg gcggcttttc ccaacagcgt gtctttgggg tcgaatccgc
     2641 gcatcccttc gggactgtcc gtgtcgccgc cgtaatattt gcaggtcagc tcgatatggt
     2701 agggtttgcc gttaagcctt gccacaaaat ccgccgcgcc ttgcgtattg ccgtccgaac
     2761 cggaaaccgt gaggttgtgc gcgagcagtt cggcgtgcgg tgcattggca aaccaaaaag
     2821 ccagcagctc ttcggcataa atgccgagac ggtggccgaa cggggcgcgt tgggcgaggt
     2881 aatccgtcag ccgcgtggga tcggcatcca atgccaaaag gtaacggaaa ccgtgttctc
     2941 ccagtagttc tcgcacgctc aattcgcagc cgctttgcca caaaggcggc gcagtcagca
     3001 gcgaggcaag gtcgcggacg ggttggctgg taagtttcca ccataatgcg tctagggcgt
     3061 aattcatgct tatccgaata tcagtcatgc cgtctgaaaa gctggaaatg ttttcagacg
     3121 gccttaatca gtctttttta tggatacggt ggctgacggt attgaaaaaa ccgcgcagga
     3181 tatcgatgtc ttcggtttgc gtattggcgc ggccgaacag gctctgcata cggcgaatca
     3241 gacgctcgcc gttgcggcgg ttgaaaaagc cgatgtcgtt catcacgctt tccatgtggg
     3301 cgaccatgcc tttgatttgc tcgtgggtcg cagcgtggtc ttcttgttga agatgggtca
     3361 tgggcgaacc ggtttggctg aagatttcgt agcacacgac ctgcacggct tgggcgaggt
     3421 tgagcgagaa atagtcggga ttgccgttga tggtcatcag tcggttgcag gcttggactt
     3481 cttcgatgct caagccgaaa gtctcgttgc cgaaaaccag tgccactttc tcgcctcggt
     3541 ttgcggtctg cagtaattcg gataccaaat cgcgcggggt ttgcagcggc gcagtaattt
     3601 cgcggcggcg gctggtcagg gcgcaggcga tggtggtgtc ggcaagggct tcgtccaaag
     3661 aagcggcaat ggtggcattt tccaaaacgt ctgccgcgcc ggaagcgagg atgaagcttt
     3721 cttccggtaa tttaaacgat tgaggatgct ccgggtcaaa cacgggcggg ttttccgtca
     3781 tcggcgttgc catcagattt ggggcgacga tggtcagttt gtgcagaccc attgttttca
     3841 tcgcgcgcgc ggcagagccg atgttggcgg gatggctggt gcgcgtgagg atgatgcgga
     3901 tgttgtccag ataagcgggc agggcgggtt tgagagtagt catgttcggt tttcggtcgg
     3961 aatggaatag ggtataatgc gccgtctttt tcgttcggac ggtcaaatgc cgtctgaaat
     4021 gttctttaac aacgtcggaa atttaacgtt gcgcctgaag cagacacgcg tcaggcggta
     4081 ttttacccga tttggcagga tttaccccaa ccaaataaac tccgattttg ccgctccgaa
     4141 ggactgtacg tccgaaccgg cggtatgcca cccttaagga aatctgatga atccgttttt
     4201 gaatacagcc tttaaagccg cccgccgtgc cggtcagatg atgatacgcg ccgcgggcaa
     4261 tctcgatgcc gtcaaaaccg acagcaaagc cttcaacgat tttgtttccg atgttgaccg
     4321 caattccgaa atcatcctgg ttgaggcttt gaaagaagcc tatccgcacc acaaaatcac
     4381 ttgtgaagaa agcggctccc acggcaaagc cgcagccgag tacgaatgga ttatcgatcc
     4441 gctcgacggc acgaccaatt tccttcacgg tcatccccaa tacgccatct ctatggcgct
     4501 gctgcacaaa ggcgtgttgc aagaagcttt ggtttacgcc cccgaacgca acgacgtata
     4561 tatggcttcg cgtggcaaag gcgcgttgct caacgaccgc cgcatccgcg tttccaaccg
     4621 catcgaactg aaccgctgcc tgattggtac cggcttccct gttgtcgatc aaagcatgat
     4681 ggacaagtat ctggcgattt tgaaagattt cttggcaaaa accgccggcg gccgtcgtga
     4741 aggtgcggct tctttggatt tgtgcgccgt agcaacagga cgttttgacg gctttttcga
     4801 gtttaacctc aaaccgtggg acattgccgc cggtgcattg attgttcagg aagcgggcgg
     4861 tatcgtaacc gatatgtcgg gtgaagacgg ctggctggaa agcggcgata ttgtggctgc
     4921 caatcccaaa gtgctggcgc aaatgttgaa aatcatttcc gcacacgttt aaatttcagc
     4981 ccgctcccgg gaatgcggga aaacgggcgg gtatgccgtc tgaagtatcg cgccgatatt
     5041 tcagacggca tttttactgg cagcgggggc aggaaggtgg aaacggctaa atgcccgaaa
     5101 ctgccgaaga tggaaaccca tatctgatta aacggagaac cctgtacgat tt
//
LOCUS       DAWGRZ010000148         5138 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000148 DAWGRZ010000000
VERSION     DAWGRZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.148"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(366..2642)
                     /locus_tag="WHT44_001694"
     CDS             complement(366..2642)
                     /locus_tag="WHT44_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ2533848.1"
                     /translation="MAQTTLKPIVLSILLINTPLLAQAHETEQSVDLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDNAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSAISEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIQYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGFHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            3064..3654
                     /locus_tag="WHT44_001695"
     CDS             3064..3654
                     /locus_tag="WHT44_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ2533849.1"
                     /translation="MLLFIDNYDSFTYNIVQYFTELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRNTMPECLEVTAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            3718..4776
                     /gene="trpD"
                     /locus_tag="WHT44_001696"
     CDS             3718..4776
                     /gene="trpD"
                     /locus_tag="WHT44_001696"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ2533850.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQIMRGQVLPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQVAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(4928..>5138)
                     /locus_tag="WHT44_001697"
     CDS             complement(4928..>5138)
                     /locus_tag="WHT44_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ2533851.1"
                     /translation="YTVVVPNAQSATLLPIIRKKVKPDGIVYTDTFRSYDVLDISEFS
                     HLRKFNGIPKEHLGLHLKKCQWHFK"
BASE COUNT         1185 a         1180 c         1451 g         1322 t
ORIGIN      
        1 cgcctgcgcg ggaatgacga cccattagtt acctaaaatt taaaaaacag aaacctttcc
       61 gcgtcattcc catgaaagtg ggaatctaga acccaaatgc taaggcgatt tatcggaaac
      121 ggctgaaacc gaacgaattg gattcccgcc tgcgcgggaa tgacgggatc ttgggtttct
      181 gcttttgatt tttctgcttt tgcgagaatg acggcgtgaa agtaagaatg atgaaacaaa
      241 aaaaatggga atgatggcat agtggtttgt tctttgtctt tgccatattt cctaacaaat
      301 tgattaaaaa gaaaaaaggt tttcagaatg ccgtctgaaa accttttttg tttgcctgtc
      361 cgattttaaa acttcacgtt cacgccgccg gtaaagctgc ggcccatttg cggcgtatca
      421 gagagaaagc tgctgtgggc gtaaacggat tggttgagca ggttgtcggc tttgacgtac
      481 caattccact cgccatagcg cgtattgcgg cggtagtttg cgccgaggtt gagcatatgg
      541 tgtccgggcg tgcgcgtttc gtagcgggcg agtttgtttt gggcgaacac gcggtagtag
      601 tccaaattgg catcgatacg gtcggtcagc gaggctttca ggtggaagcc gaggcgcgca
      661 gccggaacac ggggggcatt ttggtcgtcc tgtgcgatga aaggacggtt gccgtaggca
      721 tcttctctgc cgggtaggga aggcaggttt ttcagacggc ctcgtacata gtcgccggaa
      781 acgccgatgc ggtagcgcgg tgtcggtttg aagtagattt cgccttccgc gccgtagaag
      841 tcggcgccgg attggttgta gcgcacgagc ttcatttcgc tgtcgtcttc gatggatttg
      901 gggccgcgtc cgtcgtttaa ggtttgggcg taaatgtagt taccgaagcg gttgcggtag
      961 agtgccagat tgtattgcca gcggtcgcct tcgtagccca gcgcgagttc gatattgttg
     1021 gaacgctctt tgttgaggtg tttgttgccg acttcaaagg tgttggtggc gacgtgtttg
     1081 ccgtgtgcgt acagctcttg cgttgacggc aggcgttcct gatgggaggc ggtcaggctg
     1141 agtttgtgtt gtggcgtgaa ataccagttg cccgaaagtg cgaatgagcg ggcggtttgg
     1201 cggtgcgcgc cgaggtcggg cagggggtgg ttgtagtagt tttcccgatc aatcaatgct
     1261 ttgtcgtact gaatggaggc tttttgtttt tccacgcgta cgccgccttc aagcgtgaag
     1321 ttgtcccagt ttgcctgttc tacaccgaaa aagctgtaat gttgcacttt gttgtcaagc
     1381 agcatcggtt gtttaaccgc ttcggatatg gcagataaag cactggattt ttgttgtaaa
     1441 tattgcacgc cccagctgcc tttcagacga cctatgggtt ggtggcgcaa ctcgatgcgg
     1501 gcgttttgcg tttggttgtt aaaaaagttt tcgactgcat cgcctgcttt ttcgtcgtgg
     1561 cggtagtcgt tgcggttcag gtgtacgcgc agggcttcaa aaccggggaa cggttgcttc
     1621 cattcggcac ggagttcgta gcgtttgttg cgcaggtcta tccacggtct gccgctgtgg
     1681 gtgtgtgcgt gtgcattatc gtcgtcgtgg aagccgcagc tcaagcccgg attgtcgtaa
     1741 tcgatgtctt cttcggtcaa caggtgcgga taaagctgta aatagcgttt gttaatcaag
     1801 ctcttttgcc agatgatgtc ggcgtggcaa tcatcgtatt cgtggctgtg ggcaggcaga
     1861 ccatattggt cgcgacggtc gctgtacgct acgccgataa aacctttttc gccaacccaa
     1921 gacagcccga tgctgcccgt ttgcgaatcg gcgtggctgt cgggcaggcg tttcagattg
     1981 cggtaacgcg gtacggcgta atcccccgat ttgcggtaca gcccttccgt gtgcaataca
     2041 aagtttttgc ccaaaccgat attgatgccg ccggacgtga gtttttccag attgccgctg
     2101 ctcaaacgca atccgagttc gcccgatacg ccgttttcag gcattttttc ggggattttg
     2161 ccatcggcaa catcgaccag ccccgccaca ttgcccgagc tgtacaagag cgtaaccggc
     2221 ccgcgcagga tttcgacctg ttgcgacaag gcggtatcta ccataatggc gtgatcgggc
     2281 gaaaaatccg ccatatcgcc tgtttcgccg tgatggttca acactttaat ccgcctgcct
     2341 gtttgaccgc gaatgacggg agcagacgcg ccgccgccgt attgcgaagc gtggatgccc
     2401 ggtacgccgt ctaaagcgtc gcccaagttg acggcttttt ggcgcaaggt atcgccggag
     2461 atgattttgt cggaggcggt cgaagtgtgc aacagccccg acgtggcgcg cggacggctt
     2521 ttgccgacga cgctgaccgt ttccaaatcc accgattgct cagtttcatg cgcttgggcg
     2581 aggaggggtg tgttgattaa aagaattgat aaaacaatgg gtttgagtgt agtttgtgcc
     2641 attttggctt ctcgtcgcat ttcaaaagtt tgttattata taacattaca ttttttatat
     2701 cataagattt tgagaacact cagagggcat aggcaaaagt ttttcaaatg aaacggttgc
     2761 ggcatcgggc ggtgtccatt tgtatccgtc gtccttcggg ggcgcggttg atgttgacgc
     2821 agattccgct gtgtttgccc attatgttcg gctgcgggat aaccaaaatt tatgagtgca
     2881 taaaaacggc acgttcccga acggtcgaaa aggtggcaaa atggcgtact tgtcagaacg
     2941 cgaggctctg cgccaggttc acgagggcgc atcgggcacg ctaagatata agagggtatg
     3001 gatgggggta tcggaaatgc agttaataac aaacaaatta taaatcaata ggttaatcac
     3061 aaaatgcttt tgtttatcga caattacgac agttttactt acaacatcgt ccagtatttc
     3121 actgaattgg ggcaggaagt tgccgtgcgc cgcaacgatg atattacgtt ggaggaaatc
     3181 gaggcattga atccgcaata tctcgttatc ggccccggcc cgtgttcgcc caaagaagcg
     3241 gggatttccg tggcggcgat gcgccatttc gccggccggc tgccgattat gggcgtgtgc
     3301 ctcgggcatc agacgatagg cgaggcgttc ggcggcagga tagtccgcgc caaaacgctg
     3361 atgcacggta aggtgtcgcc cgtgtcccat tcgggcaagg gtatgtttaa gggtttgccc
     3421 aatccggtta cctgtacgcg ttatcacagc ctcgttatcg atcggaatac gatgcccgaa
     3481 tgtttggaag taacggcttg gactgaggac ggcgagatta tgggtgtgcg ccataaggaa
     3541 tatgccgtcg agggcgtgca gttccacccc gaagccctct tgaccgagca cggacatgat
     3601 atgttaaaca attttttaat cgaatttcaa aacttcaaac cgcaaaaaat ctgacgtgat
     3661 gccgtctgaa gcccttcaga cggcattttc gtccgaatat tgaacggagg acaaaaaatg
     3721 attacaccgc aacaggccat cgaacgatta atcagcaata acgagttgtt ttacgatgaa
     3781 atgaccgact tgatgcgtca gattatgaga ggacaggttc tgccggagca gatagcggcc
     3841 attttgacag gattgcgtat caaggttgaa accgtttccg aaattaccgc agctgcagcc
     3901 gtcatgcgcg agtttgcgac aaaagtgccg ctggagaatg cagaggggct ggtcgatatc
     3961 gtcggtacgg gcggggatgg cgcgaaaacc ttcaatattt cgacgacttc gatgtttgtt
     4021 gccgcagcgg caggcgcgaa ggttgccaaa cacggaggcc ggtcggtctc ttcctccagc
     4081 ggtgcggctg acgtggtgga gcagatgggc gcaaacctca acctgactcc cgaacaggtt
     4141 gcccaaagta tcaggcagac cggcatcggg tttatgttcg cgcccaatca ccacagtgcc
     4201 atgcgccatg tcgcccctgt acgccgttcg ctcggtttcc gaagtatttt caacatattg
     4261 ggtccgttaa cgaatcctgc gggcgcgccg aaccagcttt tgggcgtgtt ccacaccgat
     4321 ttgtgcggca ttttgtcgcg ggtcttgcaa caacttggtt caaaacacgt tttggttgtt
     4381 tgcggggagg gcggtttgga tgaaattaca ctgacgggca aaacacgcgt tgccgagctc
     4441 aaagacggaa aaatcagcga atacgacatc cgcccagaag atttcggtat cgaaacccgc
     4501 cgcaatttgg atgaaatcaa agttgccaat actcaggaat ctttgttgaa aatgaatgag
     4561 gtgctggaag gaagagaagg ggctgcgcgc gatatcgtat tgctcaacac cgccgccgcc
     4621 ctgtatgccg gaaatgtcgc tgcttcgctt tcagacggca tatctgccgc acgggaagcc
     4681 atcgattcag gcagggccaa atcgaaaaaa gaggagtttg tcggttttca accacaacaa
     4741 agatgccatt ttcttggaaa gatggagctt gggtgatgcc gccatgatta tggaactttt
     4801 gtggcaaaac ataagcactt cacgaagaga acttaccaaa ctgtttttat ataaaaactt
     4861 ggggctgtac tagataacca gaccaaattc ccattaacta attgtcttaa aatctgaatt
     4921 tgagattcta tttaaaatgc cattggcatt tctttaaatg cagccccaaa tgctctttgg
     4981 gaatgccgtt aaacttacgt aaatggctaa attcactaat atcaagcaca tcataactac
     5041 gaaaggtatc cgtgtataca atgccatcag gcttaacttt ctttcggata attggcaaca
     5101 atgtcgctga ttgcgcatta ggaacaacga cggtataa
//
LOCUS       DAWGRZ010000149         5095 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000149 DAWGRZ010000000
VERSION     DAWGRZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.149"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            186..1370
                     /gene="ubiM"
                     /locus_tag="WHT44_001698"
     CDS             186..1370
                     /gene="ubiM"
                     /locus_tag="WHT44_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ2533852.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNVSILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGASSL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRASNNFPPLKKLITKQL
                     TG"
     gene            complement(1559..1714)
                     /gene="rpmG"
                     /locus_tag="WHT44_001699"
     CDS             complement(1559..1714)
                     /gene="rpmG"
                     /locus_tag="WHT44_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ2533853.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(1746..1979)
                     /gene="rpmB"
                     /locus_tag="WHT44_001700"
     CDS             complement(1746..1979)
                     /gene="rpmB"
                     /locus_tag="WHT44_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ2533854.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(2229..3755)
                     /gene="farB"
                     /locus_tag="WHT44_001701"
     CDS             complement(2229..3755)
                     /gene="farB"
                     /locus_tag="WHT44_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ2533855.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFSVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYQPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     FVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(3779..4933)
                     /gene="farA"
                     /locus_tag="WHT44_001702"
     CDS             complement(3779..4933)
                     /gene="farA"
                     /locus_tag="WHT44_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ2533856.1"
                     /translation="MDTHTDETKLQNTQAKRKRRLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
BASE COUNT         1302 a         1431 c         1215 g         1147 t
ORIGIN      
        1 accccacacc cgatttgata cacattaagc tgaaagtaaa aatccgcata caccctcccc
       61 tgcatatttc ttcaacagtg ggttttgata taatcgccta tctgttacag atagttcaaa
      121 cggcattcaa acccttacaa ataccgtctg aaatcatcgg catccgtcta tgcaaaggat
      181 attttatgag tttacacagc gacatcctcg tcgtcggcgc aggcccggcg ggtttaagtt
      241 ttgcagccga acttgccggc agcggtttga aagttaccct gatcgaacgc agcccactga
      301 ctgttctgca aaatcctcca tatgacggac gcgaaatcgc cctgacccat ttttcccgcg
      361 aaatcatgca gcgtttgggt atgtgggaca aaattcccga aaacgaaatc taccctttgc
      421 gcgatgccaa agtgctgaac ggacgttccg actaccagct ccactttccc caaccgactg
      481 aagcgcgcgg cgaacctgcc gactgtttgg gctatctgat ttccaaccac aatatccgcc
      541 gcgccgccta tgaagtcgta tcacaactcg acaatgtcag cattctgacc gatacggttg
      601 tcaaagaagt caaaacatct gacaacgaag cacaagtcat tttggaaaac ggcaaaatac
      661 tgactgcacg cctgcttttg gcggcagaca gccgcttctc gcaaacccgt cgacaactcg
      721 gtatctcctc cgatatgcac gattacagcc gaaccatgtt cgtctgccgt atgaaacata
      781 ccctttccaa ccaacatacc gcttacgaat gcttccatta cggacgcacc atcgcactgc
      841 tgccgctgga agagcacctg accaataccg tcattaccgt cgataccgat aaaatcaaca
      901 gcgttcaaaa cctttcgccc gaagaattgg cggcaagcgt aaaagaacag ctcaagggca
      961 ggctcggcga tatggaattg gtcagcagca tccaccatta cccccttgtc ggcatgattg
     1021 ccaaacgttt ctacggcaaa cgcagcgcgc tgattggcga tgccgccgtc ggtatgcacc
     1081 ccgttacagc acacggtttc aacttggggc tttccagcgc agatattttg gcaaaactga
     1141 tactcgaagc cgaacagcgc ggtcaagaca tcggtgcatc ttcattattg gaaaaataca
     1201 gcagcaaaca tatgctgcac gcccatccgc tttatcacgg aaccaatatg atgctgaaac
     1261 tgtttaccaa tgagaccgca ccggcaaaac tgttgcgcgg cttggtattg cgtgccagca
     1321 acaatttccc accgctgaag aaactgatca ccaaacaact gaccggttga aaactgccga
     1381 aatgccgtct gaaacgtttc agacggcatt tttgtgttat gaaccgtttt gcggttttca
     1441 tatccttggc agacggtttg cagcagattc gcatacggca gatgtttcaa gcggaaagga
     1501 aacacgataa aaataacaaa gcctccgagc agtcggaggc tttgctttca aactgaaatt
     1561 atttcagttt agtttcttta tacactacgt gtttgcgggc aactgggtca aattttttga
     1621 tttccaattt gccgggcata gtgcgtttgt ttttggtagt ggtgtagaag tgaccagtac
     1681 ctgcactgga ttccagtttg attttatcgc gcattgcagt aatccttaat taaatagtgt
     1741 ttaaattaag cttcgccgcg agcacgcaaa tcagccaata cgacatcaat gcctactttg
     1801 tcgatggtac gcagtgcagc gttggaaacg cgcaggcgaa cccagcggtt ttcactttct
     1861 acccaaaaac gacgtgattg caagttgggc aaaaaacggc gtttggtttt gttgttggcg
     1921 tgcgatacgt tgttgccgga catcgggcgt ttgccggtca ctttgcaaac tcgtgccata
     1981 atcagtctcc aaacttctaa aatagtaaaa cgcgatttat accacgaaaa acagtgttag
     2041 ttcaagtgtt ttaaagaaaa acggctgttt tgcgcggata atccttccga ttgtcgcatc
     2101 tgtggaacgt ttgcaggtgc gaaacagatg gggcgcgatt atagcggatt tcgggaactg
     2161 ctgcattcaa aatgccgtcc gaacatattt ccagtatttt cagacggcat ttcaagtttt
     2221 caaatccctc aatgtccacc gccgccgccg ttgtggaacg gcggttttgc cagccatatg
     2281 acgggtatca tgataatgaa taacaagctg cccgccataa agatttcgtt cgagccgata
     2341 ataaaaccct gctgggtaat ggtattgttg atgatgccta gggtttgaat gtcggaaacg
     2401 ccgtgctggg acaaatgagc ggccgtttcg tgcaatgttg cggaataggg cgtgatgtgt
     2461 tcggcaaagc gtgtgtggtg caacgcttcg cgccgttccc acagggtgct gacgacggat
     2521 acgccgacac cgcccatcag cacgcgcaag aaattcgaca ggctgcctgc ggcggcaatc
     2581 tgcccgccct tcatatgcga cagtgtgatg gtggtcagcg gcagaaaaaa catggcgaca
     2641 ccgacaccct gccaaaactg cggccagatg acgttgccaa tatccatatc ggcataaaaa
     2701 tccgtacgcc aatagaaagt aaaggcaaag gtcaggaagc tggcagttac gaacaggcgc
     2761 atatcgattt tattgccgaa cctgccgatt aacggagaca ggaaaacagg caggatgccg
     2821 acaggtgcgg cggcaagccc tgcccacgtg gaggtatagc ccaggttggt ctgcaacact
     2881 aacggcagca gggtcagcgt ccccatatac accataaaac ccaatgacgt ggcaatgacg
     2941 ccgacggtaa aattccgatc tttaaacagc gataaatcga caatcggata tttttctccc
     3001 aattcccaaa caataaaata cgacaagcac accagtgcga ctacgcccaa ggtaatgatt
     3061 tctccagagg cgaaccagtc gagttcctta cccctgtcca gcatcatctg taacgcgccg
     3121 ataccgacta ccatcaatgt aagcccgaca tagtcggtcg gcattttaac ggtttccgtt
     3181 tcccgatatt tcaaatgttt ccatgtaatc catgccgata tgataccgat agggatatta
     3241 atgaagaaaa tccaacccca atgccagttt ccggaaatcc agccgccgag tatcggcccg
     3301 agaacagggg cgacaacgac ggtcattgcc cacaatgcca gtgccagcgt ccgttttgcg
     3361 ggctgatagg atgccattaa caggctttgc gacaagggaa tcagcggccc ggcgataaag
     3421 ccctgcaaga tgcggaaaac aaccagcgac tgaaggttgg gggcaatacc gcacagccac
     3481 gatgtgatga cgaaaccgac agcggcggcg gtaaacaatt tgacctcgcc gatgcgtttt
     3541 gccaaaaagc ccgtcagcgg cacggaaacg gcgtttgcca cagaaaagga agtgatgacc
     3601 cacgtcccct gagtggttgc cgcaccgagg ttgccggcga tgacgggaac ggcgacattg
     3661 gcgatagtcg tatctaaaac ttccataaat acggcaagcc ccaaagacag cgtaacccac
     3721 gccaatgccg cacccttaag cggtggataa tccattgtgt cctccgtttc agacggcatc
     3781 agggcgcgga ttgcccgagg atttcatcga ccgtccgatc gacttccgac cagtcggtac
     3841 tttccatttc cggcaatgcc gcacccggcg tttttgaaac aggcgcgcct gcggcggaag
     3901 tatccacttt aaccgtcatc gacaaaccga tacgcaacgg atgcctgtcc acatcttcgc
     3961 ggttcagcac gatacggacg gggacgcgct gcaccacttt aatccagttg cccgttgcgt
     4021 tttgcgccgg aatcagcgaa aacgcgctgc ccgtacctgc cgaaaaacct gccaccctgc
     4081 cgcgataaac aatttgtttg ccgtacaaat cggacaccag ctcggcaggc tgtccgattt
     4141 tcatatgccg caactgcgtc tctttaaaat tagcatccac ccacacatcc gacagcggca
     4201 ccaccgccat cagcggcgcg cctgccgcca cctgctgccc gacctgcacc gaacgcttcg
     4261 ccacctgacc gtccgccggc gcgcggattt gcgtccgctg aaggttcaac cacgcatctt
     4321 tcaacctgcc gattgccgtc tgaaccgccg gctgttcgcg caaagaaacc tgaccgccca
     4381 aagccgcacg tgccgaagat tcttccgcca aagccgcttt gaccgccgcc tgcgcctgag
     4441 acactgccgc acgggcgtgt gccagctctt cggcggacac cgcgcccgat tccgccaaag
     4501 cagaccggcg gcgcaaatca tcctgtgcgc gtgccaaatc cgcccggcgc aaggcaacct
     4561 gcgcccccgc ctgcgaagtg gcggcatttt gccggcggtt ttgccgcacc gcctgaacca
     4621 gctcgttttt tgcccgctcg taagccagca catcattatc gtcgtccaat accgccagca
     4681 cgtcgccttt tttcacggca tccgtatcgt cgtgcaaaac cttccgcacc gtaccgccct
     4741 tttgcggcgt aacctgaacc acgcgtccgg caacataagc gtcttccgtt tcctcttcgt
     4801 gctgccacca taaaaaaaac gccgacccgg cggctgcggc ggcaagcgcg aacagcagcg
     4861 tcaatgccgt caggcggcgt ttgcgtttgg cttgcgtgtt ttgaagtttt gtttcgtccg
     4921 tgtgcgtatc cataagattg ggttccgaat ttcctgattt catataaaca atgttctttc
     4981 aataggaagc agattctata tccgcgcaat aacgcaatca agtcagtatt ttagatgcaa
     5041 tattcaaaaa acaacgcttc aggcggcaaa actgaaaata tatttcgttt atata
//
LOCUS       DAWGRZ010000150         4995 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000150 DAWGRZ010000000
VERSION     DAWGRZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.150"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            205..2001
                     /locus_tag="WHT44_001703"
                     /pseudo
     CDS             205..2001
                     /locus_tag="WHT44_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575393.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            2014..4953
                     /locus_tag="WHT44_001704"
     CDS             2014..4953
                     /locus_tag="WHT44_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HEZ2533857.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFM
                     IVDEPHKFPTRDSAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFLDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFADLFTAYLHENAAKFPQAGIRTAVNEAYINNGLMLSRRIDSFEDEDFIR
                     FNTMTYREFLEKLAQTAKIRMQTLHQAFYCIRNELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFGEIF
                     YDSDIEHENVANNQIEGVIVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGR"
BASE COUNT         1513 a         1171 c         1200 g         1111 t
ORIGIN      
        1 aacccaagcc ctgatctaag cgacacagcc ggggcgaaca ctgaggcaat ctacacttca
       61 gacggcatta ccgccaactc tacccaactc gaacagctca aaaaactgtt tcccgcctgt
      121 tttgacgcag acggaaattt ccttatcgac agattacaag ccgaaatcgc gccgcagacc
      181 gacatcggac gcgaatttta cgaaatgaac tggctgggca aatcatatgc tcgcctgctt
      241 cgcaacctgc cgcccgaaac cctgataagt gaagacaaga cgcacaacgc caagcccgaa
      301 aatgcaggca gccaaaacct gctgattcgt ggcgacaatc tagaagtgtt gaaacactta
      361 aaaaacgcct acacaaacag cgtgaagatg atttacatcg acccgcccta aaacaccgga
      421 tcagacggct ttgtctatca ggacgaccgc aaattcacac ccgctgaact tgcccgccta
      481 gccaatattg atgaagacga agccgcgcgg attttagatt tcaccgacaa aggctcaaac
      541 tcgcacagcg catggctcac ctttatgtat ccgcgcctgt atgtcgcccg cgaactgtta
      601 aaggacgacg gtgtgatttt tatctcgatt gacgataacg aagcggcgca gttgaaattg
      661 ttgtgtgatg aagtgtttgg ggaagggaat tttgttgcac aattgccttg gcgaaaaaga
      721 acagctaaat cagatgtgcc ttttggtatt tcgcaggatt atgaatggat atttgtattc
      781 gcaaaatctt gccaatttat tgcagcaact aaaggcaagg aacgacgcta ttatgagact
      841 gatgatttcc ccgatcgtcc ttggcgtacc cacgacttaa cgaaacaaac aactgcggcg
      901 gaaaggccaa atagtttttt cacaatggtt gatcccaaga caggaaaaaa atatccagca
      961 aatccaaatg caacttggcg tgtaaccaaa gatacatttc aagattatta taataaagga
     1021 aaaatcgttt tccctgatga ttatgatttc ttaaatatca gcaatccagt tatgcgttac
     1081 tttaaagatg acgacatgaa aaaagctggc gaggattttg gcaaagtagc tgtaagcagt
     1141 agattacctg aaaatgttgg tacattagcc gatgcagtag ccgaatattt ggctattttt
     1201 agtaggacgc tacctgaaaa tatcggaatg actaaagaag gcacaaaaga aatcacagat
     1261 ttatttggta gcaaaatatt taccttcccc aagcctagtc aattgattaa atttttagtt
     1321 tcaataagtt caaagagtaa tgacctaatc ctagacttct tcgcaggcag cggcacaacc
     1381 gcccacgccg tgatgcagct taacgccgaa ggacaaaacg gtaaccgccg ctatatctgt
     1441 gtacagcttc ccgaaaaaac cgctgaaaaa tccgaagccc gtaaagcagg ctacccgacc
     1501 atcttcgaca tcaccaaagc ccgcatagaa aaagccgccg ccaaaatccg cgtcgaacat
     1561 cccgattaca cgggcgattc gggcttcaaa atctttcaaa cggcagacaa tttcaggcag
     1621 catccggaca aggatttttc gcccgaacaa ccggatttgc cgcttaacga tgaattaagc
     1681 gaagaacagc tgcaaacgct tctgaccacc tggacgctgt atgacggggc ggcactgacc
     1741 acgccggttg agcctgtgcg gttaggggct tacacggcgt atctgtgcga aaaacggctg
     1801 tatctgatga atgccggttt tacttccgcc gatttgttgg cgtttatccg caagctggac
     1861 gacgatgcgg atttcaatcc caaccgcgtg attgtattcg gcagcaatat ggcaagcgcc
     1921 atgcagcacg aacttgacca ggcggttcgc ggttatgcca ataaaaaaga gattgagttg
     1981 aatgtggtta tcagggtttg acggaggcaa ttcatgagcg gttttaatta cgagaaaaac
     2041 cagccgcacc aaatgcgggc ggtttcggcg gttttgggcg tgtttgacgg ggcaacgccc
     2101 aaatatcgga cggcagacga aaatcccgaa cttttgtttg ctgcaaaaca atacgcaaac
     2161 aatatcctga aagtgcaaag ccaaaacggt atagacggcc gattccccga ccgttcggac
     2221 gaccaaaata tccttgatat ttccatggaa acgggcacgg gcaaaaccta tacctacaca
     2281 caaaccatgt tcgagctgca ccgttggctg ggcgtgttca aatttatcgt ggtcgtgccg
     2341 actttgtcca ttaaggcggg aacacagcag tttttgcaaa gcaaggcttt ggcagagcat
     2401 tttgaacagg atttcggcgg cgattatgaa ggcgtacgcc tgaaaaccta tgtggtggaa
     2461 agcgcgaaaa agaataaggg caaaaagtcc aatgcgccca taacgattga gcaatttgtc
     2521 aaagcggaaa acaaaaagga aattcatgtg ctgctgatta acgcgggcat ggttaattcg
     2581 tcgtccatga acgatacggg cgacaaggca ttgaaggatt tgtttgacaa tcccgttgat
     2641 gcattggctg ccgtgcgccc gtttatgatt gtggacgaac cgcataaatt cccgacccga
     2701 gatagcgcga aaacgtgggg caatatcaaa cgcttaaaac cgcaatatat tttgcgctac
     2761 ggtgcaacat ttaacgatga atattacaac ttgctttacc gtttgacggc agtagacgcg
     2821 tttaacgacg ggctggtcaa aggcgtgcgc gtgtttcagg aagaaatgca gggcggcatg
     2881 gatgcggcgg taaaactggt gtcgtcggac ggcaaagaag cgaaatttga attaaacgaa
     2941 aaggacaaaa agcagacgtt caaactggca aaaggcgaag atttggcgca aatccatccg
     3001 gctatttcgg atttgaaaat cgacaaaatg aataaaaccg tggtggtgtt aagcaacggc
     3061 ttggagttga aaacgggtgc cgtcatcaac ccttattcct attcgcaaac ggtgcaggat
     3121 gcgatgatgc agcgggcggt tgccgaacat ttcaagctgg aacgcgcgct tttggcagaa
     3181 cgcgcgccac agcccaaaat caagccgctg acgctgtttt ttattgacga tatcgcgggc
     3241 taccgcagcg gcaacgagct ttcaggcagc ctgaaagata aatttgaaag ctggattcgc
     3301 gcggaagcgg cacgccgtct gaaaacggaa agcgacccgt tttaccgcga ttacctgcaa
     3361 aagacgttgg acgatgtatc cgcctgccac ggcggctatt tttccaaaga caatacagac
     3421 agcgacgata gaatcgagca ggaaatcaat gaaatcctgc acgataagga aaaactgctg
     3481 tctttggaca acccgcgccg ctttattttt tccaaatgga cgctgcgcga aggctgggac
     3541 aatcccaacg ttttccagat ttgcaaactg cgttccagcg gcagcacgac ttccaagctg
     3601 caagaagtcg gacgcggcct gcgcctgccg gtaaacgagc tgatggcgcg ggtgcgcgat
     3661 gtaccgtaca aactgaatta ttttgtcgat agcagcgaaa aagactttgt gaagcagctt
     3721 gtcggcgaaa tcaacgacaa ttcttttcag gaagaaatct ccaaaaagtt taccgaagag
     3781 ctgaaacaaa aaatattgca aaaatacccc gatatcaaac cgctggtatt ggtaaaccaa
     3841 ctgtttttag atggcatcat tgacgacaat gaaaactttg ccgaagacgg ctatgacaaa
     3901 ttaaaagccg cctatcccga agccttcccc aaaggtttgg acaaaggcaa agtcagcaac
     3961 gccaaagacg aaggcaaaga caccatcatc atgcgcgaag gcaaatatga agaactcaaa
     4021 gccttgtggg agctgattca ccataaagcc gttttgcagt ataaaatcaa ggatgaagcc
     4081 gaatttgccg atttatttac cgcctatttg catgaaaacg ctgccaaatt cccacaggca
     4141 ggcatacgca cggcggtaaa cgaagcttat atcaacaacg ggcttatgct ttcccgccgc
     4201 atagacagtt ttgaagatga agattttatc cgtttcaaca caatgactta ccgagagttt
     4261 ctggaaaaac tggcacaaac ggcaaaaatc cgtatgcaga ctttgcatca ggcgttttac
     4321 tgcatccgca acgaactgaa cattggcgat tttttgaata tgcaaaccat cgcccaaatc
     4381 aaaaacggct tcaaccggtt tttgcttcat cattccttcc ataaattcga actggattac
     4441 cggcttgtcg gcagcaaaat ccatccgacc aaatttacca ataaagacgg caaaccgcgc
     4501 gcggtgaaaa aagcagattt gggcagattt gaagatacgg agcaccggcc tgccgccggc
     4561 tatctcttcg gcgagatttt ctacgattcg gatatagaac atgaaaatgt cgccaacaac
     4621 caaattgaag gcgtaatcgt atttaccaaa ataccgagaa actccatcaa aatccctgtt
     4681 gccggcggcg gcacgtattc gcccgatttt gcctatatcg tgaaaaccaa aagcggcgag
     4741 atcctgaact ttgtgattga agccaaaggg actgacgggg cggaagattt gcgaaaaagc
     4801 gaagagcgga aaatcaaaca tgccgaaaag ctgtttgccg agatttccaa agaaatcaag
     4861 gtggtgttca aaacgcagtt tgacggcgag aggatagccg aactgatcgg gcaaaatatg
     4921 cccgcaggcg ggcattctga aaacgggcgt taaagaacga atgttcgggg cgcgtgccgt
     4981 ctgcttcggg atttt
//
LOCUS       DAWGRZ010000151         4915 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000151 DAWGRZ010000000
VERSION     DAWGRZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.151"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(88..1095)
                     /locus_tag="WHT44_001705"
     CDS             complement(88..1095)
                     /locus_tag="WHT44_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ2533858.1"
                     /translation="MYSNNAITNNRNNITLGAYRDAIISIFSDENVVRAIGTINSMYE
                     EYLTLSHKIDQRAFLREGERLSAKAREFQRTAEEAQKSLKEGIGRAASKFRGNSKEAF
                     VARKVQSDLQKALNASNYNKQQYARRAATALENASKSKVMAANLGKFTYGATVAMGTL
                     SMGSAWWKALDEEGNWSDVGLSSASFLGGLMGFQWGAALGVGAFGLLTGGAGWLVVGT
                     IIGGGSGAWWGSLGAEKAWRWFEDFLNLNRSDKFHVTDPLALDLDGDSIETVATKGLA
                     GALFDHRSQGIRTATGWIAAYDGFPVRKLNSNGGIISTTDTIFQSLHTWLDHQPNDTC
                     H"
     gene            2369..4501
                     /locus_tag="WHT44_001706"
     CDS             2369..4501
                     /locus_tag="WHT44_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ2533859.1"
                     /translation="MSIVSAPLPALSALIILAHYHGIAANPADIQHEFCTSAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPVKRLAMATLPALVWCDDGNHFILAKTDGGGEHAQYLIQ
                     DLTTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
     gene            4725..>4915
                     /locus_tag="WHT44_001707"
     CDS             4725..>4915
                     /locus_tag="WHT44_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ2533860.1"
                     /translation="MSTFFRQTAQAMIAKHINRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YLRDHRGRPAYPLLSMFKAV"
BASE COUNT         1269 a         1102 c         1098 g         1446 t
ORIGIN      
        1 tacctagccc gaaccttctt ataatttgta ctatgtgatc attcatttca atatccattt
       61 aagctttcaa ttaattttct atatttttca atggcatgta tcatttggtt gatgatcaag
      121 ccatgtatgc aaagattgga atatggtatc tgtcgtgcta ataatgcccc cgttactgtt
      181 taatttgcgc acaggaaaac cgtcatatgc agcaatccag cccgtggcgg tgcggatacc
      241 ttggctgcgg tggtcgaaca atgcacctgc aaggcctttg gtggcaacgg tttctatact
      301 gtcgccatct agatctaggg caagggggtc ggttacgtgg aatttgtcgc tgcggttaag
      361 atttaagaaa tcttcaaacc atctccaagc tttttcagct cctaatgatc cccaccaagc
      421 tcctgagcca cctccaatta tagttccaac tactagccaa cctgcgccac cagtaagtaa
      481 accaaaagcc ccaacaccca aagccgcacc ccattggaag cccattagcc cacccaaaaa
      541 agaagctgat gagagaccaa catcactcca atttccttct tcatccaagg ctttccacca
      601 agcacttccc atactcaaag ttcccatggc aacagttgcc ccataagtaa acttacccaa
      661 attcgctgcc ataacttttg attttgaagc attctctaac gctgttgccg cacgtcttgc
      721 atattgctgc ttgttatagt tgctagcatt caatgctttt tgcaaatcac tttgtacttt
      781 tcttgcaaca aatgcctcct ttgaattacc acgaaattta ctagcagctc ttccaattcc
      841 ttcttttaag cttttttgtg cctcttctgc tgtgcgttgg aattctcttg ccttagcaga
      901 cagacgctca ccctccctta aaaatgctcg ttgatctatc ttgtgcgaaa gggtcaaata
      961 ttcttcgtac attgagttaa tcgtaccaat cgccctaacc acattttcat cagaaaatat
     1021 ggaaataata gcatccctat acgcacctag agtaatattg tttctattat tagttatagc
     1081 attattcgaa tacataatag cacctccaaa cttagaactg aatacgttta tattctctgt
     1141 gtaataatat caaataatta attaatggat aagtaagtcc gaaaaaataa caactaaaat
     1201 aagcaatata ctgattctct cctttattaa ttagatatat tgaaaaacaa ataaaaaata
     1261 aacagaaaac attcatgtga aaaagccacc tcatattctt atttataagt ttaaaataac
     1321 taggcattgt atatttaaaa atcggtacat ccaacggaaa caaaccacat aaaaattttt
     1381 ctttattctt tattaaagac atagttttat ttttcaaaat tagtccagat aatatactaa
     1441 caattactat taaaacaata agcacagcgt aattttttat aaaattcatt aaaataataa
     1501 cgaaaatact aaatagatta catactgtaa attgaaatat aaaatattca gtatttaatc
     1561 cattttcaac cgcgagtttt ttaaaatatc cttgctcgaa aaaattaaaa aaatccatta
     1621 atactttcct ctcaagaaaa ctaattttag tttagttaag ataaaatgca atattacctt
     1681 ttctccataa aagtatggag cttatataca taaattatgt gcgaggtagc ctgaaaaata
     1741 tccaatacaa gatagtagga gatgtttccc ttatcactat tggccaatac ccaattggca
     1801 ggcactctat ttccaaaatt ttctcaagaa tagttaaata ttattaatta gtttaaaaac
     1861 aataacatac gtttgtattt atccgttttg gtctgcctga aacagactct atgtccatac
     1921 acggacatgt agacagtttc cttgttattt tggttgctcg tcattagtga tgatacttta
     1981 ctcctctctt aaaaaatcgt caagaactgt tatcaatctt taaagtcttt tttaaacaaa
     2041 tatatatacg ttcaggctgt ctgaaacaca aaaaaatgat gttacccgac cgtgacagag
     2101 tcggggcagc cggtacctta accctgcacc ttaaccctgt gccttaacgg tatgcaggag
     2161 gcacgagagg tgcgaggcat cctcatctaa ctcgattcct aagccgtttt accttcctga
     2221 aacgagaact tttccatact ttctctttac aattcccata tacatcctta caatgtgttc
     2281 tgctgtttta tttatatatt tctttctact gaatatattc cttttccagc cagccgttat
     2341 ccacatccaa tctaactata ttttatttat gtctatcgta tccgcaccgc tccccgccct
     2401 ttccgccctc atcatcctcg cccattacca cggcattgcc gccaatcctg ccgatataca
     2461 gcatgaattt tgtacttccg cacagagcga tttaaatgaa acgcaatggc ttttagctgc
     2521 caaatctttg ggattgaagg caaaggtagt ccgccagcct gtcaaacgtt tggctatggc
     2581 gactttaccc gcattggtat ggtgtgatga cggcaaccat tttattttgg ctaaaacaga
     2641 cggtgggggt gagcatgccc aatatctaat acaggattta actacgaata agtctgcggt
     2701 attgtctttt gccgaatttt ctaacagata ttcgggcaaa ctgatattgg ttgcttcccg
     2761 cgcttcggta ttgggcagtt tggcaaagtt tgactttacc tggtttattc cggcggtaat
     2821 caaataccgc cggttgtttt ttgaagtatt ggtggtgtcg gtggtgttgc agctgtttgc
     2881 gctgattacg cctctgtttt tccaagtggt gatggacaag gtgctggtac atcggggatt
     2941 ctctactttg gatgtggtgt cggtggcttt gttggtggtg tcgctgtttg agattgtgtt
     3001 gggcggtttg cggacgtatc tgtttgcaca tacgacttca cgtattgatg tggaattggg
     3061 cgcgcgtttg ttccggcatc tgctttccct gcctttatcc tatttcgagc acagacgagt
     3121 gggtgatacg gtggctcggg tgcgggaatt ggagcagatt cgcaatttcc tgaccggtca
     3181 ggcgctgact tcggtgttgg atttggcgtt ttcgtttatc tttctggcgg tgatgtggta
     3241 ttacagctcc actctgactt gggtggtatt ggcttcgttg cctgcctatg cgttttggtc
     3301 ggcatttatc agtccgatac tgcggacgcg tctgaacgat aagttcgcgc gcaatgcaga
     3361 caaccagtcg tttttagtag aaagcatcac tgcggtgggt acggtaaagg cgatggcggt
     3421 ggagccgcag atgacgcagc gttgggacaa tcagttggcg gcttatgtgg cttcgggatt
     3481 tcgggtaacg aagttggcgg tggtcggcca gcagggggtg cagctgattc agaagctggt
     3541 gacggtggcg acgttgtgga ttggcgcacg gctggtaatt gagggcaagc tgacggtggg
     3601 gcagctgatt gcgtttaata tgctctcggg acaggtggcg gcgcctgtta tccgtttggc
     3661 gcagttgtgg caggatttcc agcaggtggg gatttcggtg gcgcgtttgg gggatattct
     3721 gaatgcgccg accgagaatg cgtcttcgca tttggctttg cccgatatcc ggggggagat
     3781 tacgttcgaa catgtcgatt tccgctataa ggcggacggc aggctgattt tgcaggattt
     3841 gaacctgcgg attcgggcgg gggaagtgct ggggattgtg ggacgttcgg ggtcgggcaa
     3901 atccacactc accaaattgg tgcagcgtct gtatgtaccg gagcagggac gggtgttggt
     3961 ggacggcaac gatttggctt tggccgctcc tgcctggctg cggcggcagg tcggcgtggt
     4021 cttgcaggag aatgtgctgc tcaaccgcag catacgcgac aatatcgcgc tgacggatac
     4081 gggtatgccg ctggaacgca ttatcgaagc agccaaactg gcgggcgcac acgagtttat
     4141 tatggagctg ccggaaggct acggcaccgt ggtgggcgaa caaggggccg gcttgtcggg
     4201 cggacagcgg cagcgtattg cgattgcccg cgcgttaatc accaatccgc gcattctgat
     4261 ttttgatgaa gccaccagcg cgctggatta tgaaagtgaa cgagcgatta tgcagaacat
     4321 gcaggccatt tgcgccaacc ggacggtgct gattatcgcc caccgtctgt ccaccgttaa
     4381 aacggcacac cggatcattg ccatggataa aggcaggatt gtggaagcgg gaacacagca
     4441 ggaattgctg gcgaagccga acggatatta ccgctatctg tatgatttac agaacgggta
     4501 gaaggcgggg gatgggcgtg taggctgctt ttttcgtaac gttctgcgtt ttcagaccgt
     4561 cgtttactgg gtttactgac tgaaatgaaa ccaaagtgca ggctgccgga aagacttttt
     4621 ctgtcttttt aggcagcctg cacttctttt tcactcatct agagaccttt gcaaaattcc
     4681 ccaaaatccc ctaaattccc accaagacat ttaggggatt tctcatgagc accttcttcc
     4741 ggcaaaccgc ccaagccatg attgccaaac acatcaaccg cttcccgcta ttgaagttgg
     4801 accaggtgat tgattggcag ccgatcgagc agtacctgaa ccgtcaaaaa aaccgttacc
     4861 tccgagacca ccgcggccgt cccgcctacc cgctgttgtc catgttcaaa gccgt
//
LOCUS       DAWGRZ010000152         4886 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000152 DAWGRZ010000000
VERSION     DAWGRZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.152"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(92..574)
                     /locus_tag="WHT44_001708"
     CDS             complement(92..574)
                     /locus_tag="WHT44_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ2533861.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHVGDLVTFYASVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASGDVSCGC
                     "
     gene            complement(727..888)
                     /locus_tag="WHT44_001709"
     CDS             complement(727..888)
                     /locus_tag="WHT44_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533862.1"
                     /translation="MSEQSEKNHNPLLEDERKNPVYRMGQAVAGFMLVVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(962..1423)
                     /gene="recX"
                     /locus_tag="WHT44_001710"
     CDS             complement(962..1423)
                     /gene="recX"
                     /locus_tag="WHT44_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ2533863.1"
                     /translation="MKPQKSLRARAMDILSRQELSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKLAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDDGWEEDC"
     gene            complement(1491..2198)
                     /locus_tag="WHT44_001711"
     CDS             complement(1491..2198)
                     /locus_tag="WHT44_001711"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ2533864.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVVTNKNEILASELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKEE"
     gene            2600..3865
                     /locus_tag="WHT44_001712"
     CDS             2600..3865
                     /locus_tag="WHT44_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ2533865.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDDTVKTFTGYRSQHNNAVGPYKGGVRFHPSVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGITLDPRDYSEAELERIARAYAEAIAPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITNE
                     AGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYREFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(3923..4729)
                     /locus_tag="WHT44_001713"
     CDS             complement(3923..4729)
                     /locus_tag="WHT44_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ2533866.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKTDFPDQSVCQKQANYSTLTFQIGHSHH"
BASE COUNT         1066 a         1109 c         1421 g         1290 t
ORIGIN      
        1 aatttaatcc actatatttg cttgatttca tccgtttgca atggaaacgg atggcgcgat
       61 gtgcctgtct ttcagacggc atagtccgcc gtcagcagcc gcaggacacg tctccggagg
      121 cttgcaggct gatgtctctg cgttttttgg ctttttcgta gcggcagcgt tggcggtcgg
      181 tcaggatttc cagcggaggg acagggacgg gtttgccgtc tttgactgca accatggtga
      241 agtagcagct gttggtatgg cggatttctc ccgtacggat gttttgtgct tcgacacgga
      301 tgccgatttc catagaggta cgccccgtgt agtttacgct ggcgtagaaa gtaaccaggt
      361 cgccgacatg gatgggttct ttaaacagga ctttgtcaac cgacagggta acgcaataat
      421 tgccgctgta acggctggcg caggaatagg cgacttggtc gagcaggagc aggagttcgc
      481 cgccgtgtac gttgccgctg aaattggcgg tgtccggcat catcagttcg gacataatga
      541 gttcgtgcga aggcagttgg cgttgttgtg tcatagtgta cctgttttaa gaaagggtat
      601 tttgaaatgg gtgtggaaaa attttaggga atgggaaagt ggaattcaag ctaaaaaaag
      661 ctatgatggg atagcctgcc gaaatgggct gccgatgccg tctgaaggtt tcagacggca
      721 ttttgtttag gaaagccaaa aacggaagac taggaaaaac acgagtgcca atacgcctgc
      781 ccaaacgacg agcatgaatc cggcaactgc ctgacccata cggtaaaccg ggtttttgcg
      841 ttcatcttca agaagtgggt tgtgattttt ctcggattgt tcggacattt tattctcaat
      901 cggaaaagtt ggaaatggag gtaaggttac gccgtcatgc aaaaagattc aaggattcag
      961 ttcagcagtc ttcctcccag ccgtcatccc aggcatgttt caatgccgtc tgaacggtat
     1021 cggcatcaaa accgcgatag gcgaggaagc gtgcctgttt ttgtttttct ttaaggtcgg
     1081 cggccggatg tttgaatttt ttacgcaaca cggctatggc ggccagtttt tcgcttgagc
     1141 ggtcgggaag caggttgcgg ctggtttctt catctatgcc ctgttgcgcc aaagcctgtt
     1201 tcagcctcaa tgaaccgtgt ttgcggcttt tgctgcggat ataggcttcg gcatagcgca
     1261 aatccgactg ccagttgcgt tcggcaaatt cgtttaacac gttttccaac tcctcttcgc
     1321 tttcggcgtg cggtgcaagt ttgcgtttca gaccgatgcg gctgagttct tggcgcgaga
     1381 ggatgtccat cgcgcgggcg cgtagggatt tttgcggttt catggcggcg gtttcggcgt
     1441 ggggcaggtt tcagacggca tagcggcgca aaccggagcc gtccgaatgc ctactcttct
     1501 ttgtttttct gaggttgcag gttttcgtaa atttcgggca gcgagccgat aatccagtcg
     1561 gggcgggtcg catcgtcttg cgagagcagc gtcatatcgc cgtaaccgaa ggtaacgccg
     1621 acgctcaggc agccggcggc tttggcggcg atgatgtcgt tgcgcgagtc gccgaccata
     1681 accatgtttg caacatcgat acccaaaact tcggcggcgt gccgcagcgg cagggggctg
     1741 ggttttttct cgggcaggct gtcgccgccg agtatcaggc taaaatagtc ggcgagtccc
     1801 agttgtttta gaagctcgga ggcaaggatt tcgtttttgt tggtaacgac ggcgagcggg
     1861 atgcccaaag atttaagcaa tgccagcccg gcttcggttt cgggataggg gcgggtgaag
     1921 acgctcaaat ggtcgcggta gtatttcata taggatacga aacctttttc ccacagttcg
     1981 gaatcggctt cgcggtcgcg gtcgttggtg aggacgcggt gaaccagttt gccgatgccg
     2041 tcgcccacat agctttcgac cactttggca ggcagcggtt tcataccgag ttgttccaac
     2101 atcgcttctg cggcggcggc aaggtcgggg acggaatcgc acagtgtgcc gtccaaatcg
     2161 aaggcgacgg cttggacgtg ttcgatggct gcattcataa tccggtttcc tgtcgggttg
     2221 aaaaggttta ttttaacatc gtttgcacag ggcttctaat gtatggattg ttaacaatcg
     2281 aaacaggttt tgccggcggt gtttccggat ttcggaacgg agtcgggaag ggtgtgcaaa
     2341 tcccggcgcc gttccaatta aatttttaaa aaataaaagg attcttatat ctttatttaa
     2401 ttggacattt gctttttgga tatgtgttcc gcttttttca gacggattcc gtgcggcttg
     2461 ggaaataaaa tcatatatag attaatcaaa tcgggccgat tccaactaca cgctattaca
     2521 ttttgcttga aaattggtta tagttgcgaa cctgatggct tgttccattc cgaacctctc
     2581 tttgtgaaaa ggaagcaaaa tgtctgaagc ccttgcaaaa gaaaccctca atccgtttga
     2641 aatcgcgcgc aaacaggtga aaaccgcctg tgaccggctg aaaaccgatc cggccgttta
     2701 tgaaatcctg aagagtccga cccgcgtgtt ggaagtcaat tttcccgtca aactcgacga
     2761 cgacacggtc aaaaccttca cgggctaccg ttcgcaacac aacaacgccg tcggccccta
     2821 taaaggcggc gtgcgcttcc atcccagtgt gaatttggac gaagtcaaag ccctgtcaat
     2881 ttggatgacc atcaaatgct gcgttgcggg tattccttac ggcggcggca aaggcggcat
     2941 taccttggat ccgcgcgatt attccgaggc ggaactggaa cgcatcgccc gcgcttatgc
     3001 tgaagcgatt gccccgctga ttggcgagaa aatcgatatt cccgcccccg atgtgaatac
     3061 caacggcaaa attatgtcgt ggatggtgga tgcttatgaa aacgtggtga aacattccgc
     3121 gccgggcgta tttaccggaa aaccggttga gttcggcggc tctttggcgc gtaccgaggc
     3181 taccggttac ggcgtgaacc tcgccgccgt ccaagctttg gaaaaactgg gcaaggacgt
     3241 gaagggtgcc acttacgcca ttcaaggttt cggtaatgtg ggttaccaca caggttatta
     3301 cgcgcatcaa tccggcgcga aagttgttgc ggtttctact gttgacgttg ccatttacaa
     3361 cgaaaacggt ttggatatgg aagctttgtt taaagagttc caagaaaaag gcttcatcac
     3421 caacgaagcc ggttacggca aagaaattac caacgccgaa cttttggctt tggatgtgga
     3481 cgtactcgcc ccttgcgcgc tggaaaacca actgacttcc gaaaacgccg gtaaagtccg
     3541 cgcgaaaatc gtagtcgaag gcgcgaacgg cccgactacg cccgaagccg acgtgatcct
     3601 gcgtcaaaac ggcgtattgg tcgtgcctga tattctggcg aactgcggcg gcgtggtcgt
     3661 ttcctatttc gagtgggtgc aaaacctgca aggctattat cgggagtttg acgaagttca
     3721 agagaaagaa accgtcgtcc tgcgacgcgc gttccgcgat atatggaact tggcgcaaga
     3781 gtatgatgtc gatttgcgaa ccgcgtctta catgatgagt atccgccgtg ttgaaaaagc
     3841 gatgaaactg cgcggttggt attgatgttt gaacaaaaaa tgccgtctga agccttcaga
     3901 cggcattttt gtgcgggcgg gattaatggt gcgaatgacc gatttggaag gttaaagtcg
     3961 agtagttcgc ctgtttttgg cacacgcttt gatcggggaa gtcggttttg tgttcgacat
     4021 tggctttcca gaagccttgg cgcaagggga tgatgtccac ttcgcctttg tcgtctgtac
     4081 tgtcggagaa agcctgtgct tcggttttgt gcgttttgct gcggtcgctg gtgtcgaagc
     4141 cgtcaaaggt ggcggtaacg gtggcattgg gcagcggttc gccacggaac agaacgcgga
     4201 ctttgaagcg ttcgcctacg tgaatgttgg cgggattgtc cagcgggacg atttccaagt
     4261 tttgtccgac cggtttggtg atgatggcgg tgtccgcgct ttcgtgtccg acgttgacga
     4321 tgtttttgcc gaacattcgg gtttgttcgc aatagcttgc gtcaggcatt tctttgatgc
     4381 ccgcctgttt ccagcctgct ttgttttttg accagaaagt aggctgatat tcggcgatga
     4441 cgaggtaact gccgtcctta acgggacggt tgcttcggta ctggtagttg tatgtgccgc
     4501 gttgaatcat gttttccttg cctttttcgg taaccagctg catcggtttg ctgaagatgt
     4561 gcaggcggtc tttggcgatg ggttcgagtt cgggaaattc gccgtagccc aagtcggctt
     4621 taaggtattc gccgccgtgc gtgtgggcgg tttcgaccca gacgcggtgg gcgtgggcgg
     4681 atgtggcaaa tagggcggaa acggcgagca gtgtcaatgt tttcttcatg gtgcagcctt
     4741 tgtctcgtgt gggttaaaga ttttgttatg ttataacaaa ataggtatcg tgtgaagacg
     4801 gatttgctgt ctgaaaactg tctttttgta tagtggatta acaaaaacca gtacggcgtt
     4861 gcctcgcctt agctcaaaga gaacga
//
LOCUS       DAWGRZ010000153         4728 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000153 DAWGRZ010000000
VERSION     DAWGRZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.153"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            166..945
                     /gene="pgeF"
                     /locus_tag="WHT44_001714"
     CDS             166..945
                     /gene="pgeF"
                     /locus_tag="WHT44_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ2533867.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAEALGGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAATAFEGIGSGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            997..1476
                     /locus_tag="WHT44_001715"
     CDS             997..1476
                     /locus_tag="WHT44_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533868.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALR
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1478..2476
                     /locus_tag="WHT44_001716"
     CDS             1478..2476
                     /locus_tag="WHT44_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ2533869.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLGLLYPKGHTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVLRVCRLLDGLREEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKRLVVSLAE"
     gene            2597..3016
                     /locus_tag="WHT44_001717"
     CDS             2597..3016
                     /locus_tag="WHT44_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533870.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3092..3607
                     /locus_tag="WHT44_001718"
     CDS             3092..3607
                     /locus_tag="WHT44_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ2533871.1"
                     /translation="MMTLKTAPLKRRFAAMLYEMLLVGAATCLAALIAGIAAIFLNPV
                     SIAVSALVTSILIMGAWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3691..4575)
                     /locus_tag="WHT44_001719"
     CDS             complement(3691..4575)
                     /locus_tag="WHT44_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ2533872.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDLRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDAENGSQSLELNRDLVGQLAEINKDLR
                     KHHDLAKLGVADILRFPGVLASQRENTEELAKSITELTEKALKDFTAARKREGKKLGE
                     HLLQRLEAMEEIIDALSELFPTLLETHKEKIRARLVEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
BASE COUNT         1058 a         1252 c         1327 g         1091 t
ORIGIN      
        1 ataagggcat cattcctgca ccggcaagaa tccgaacccg aacgtttgaa aacaatcccg
       61 aatctccgaa ttcccgcctg tgtgggaatg acgaaaaaac aagcattcat ttgccccgaa
      121 ggcagttaat caaccctttc cgccacacac ctattccaat atccaatgaa aaccatcaca
      181 gaaaccctaa atctcgcccc gaaaggcaaa aacttcctga ccgccgattg gcccgcgccc
      241 gccaatgtga aaaccctgat taccacgcgc aacggcggcg tgagccaagg tgcgtatcag
      301 agtttgaacc tcggtacgca cgtcggcgac aatcccgaag ccgtgcgccg caaccgcgaa
      361 atcgtgcaac agcaggtcgg actgcccgtt gcctacctca atcaaatcca cagcaccgtc
      421 gtcgtcaatg ctgccgaagc gttgggaggc acacccgatg cggacgcttc cgtagacgac
      481 acgggcaagg ttgcctgtgc cgtgatgacc gcagactgcc tgcccgttct attttgcgac
      541 agggcgggta cggcggttgc cgccgcacac gcgggctggc gcggtttggc gggcggcgta
      601 ctgcaaaaca ccatagccgc aatgaaggtt ccgcccgtcg aaatgatggc gtatctcggc
      661 cccgccatca gtgcggatgc gtttgaagtc ggacaggatg tgtttgatgc gttctgcacg
      721 cccatgcccg aagccgccac cgcatttgaa ggcataggca gcggcaaatt ccttgccgac
      781 ctttacgcgc tcgcccgcct gattctgaag cgcgaaggcg tgggcggcgt atatggcggc
      841 acgcattgta cggttttgga acgggatact ttcttttcct accgccgcga cggagcgaca
      901 gggcgtatgg cgagcctgat ttggctggac ggcaatgccg tctgaacacg ccgctgatat
      961 aatctaccga ctttgtgttt ttgagaaagg caagccatga acaaactgtt tcttactgcc
     1021 gcagtgctga tgctgggcgc gtgcggtttc cacctgaaag gtgcagacgg catttctccg
     1081 ccgctgacct accggagctg gcacatcgaa ggcggacagg cattgcggtt tcctttggaa
     1141 accgcgctgt atcaggcttc gggcagggtg gacgatgctg ccggcgcgca gatgaccctg
     1201 cgtatagaca gcgtttccca aaacaaggaa acctacaccg ttacccgtgc ggcagtcatc
     1261 aacgaatatc ttttgatatt gacggttgaa gcgcaggtat tgaaacgcgg cgagccggtc
     1321 ggtaaaccga tgaccgtgtc cgtccgccgc gtccttgctt atgccgacaa cgagatcttg
     1381 ggcaaacagg aagaggaagc ggcattgtgg gcggaaatgc ggcaggatgc cgccgaacag
     1441 attgtccgcc gcctgacctt tctgaaggcg gaatgacgtg gcggcacata tcggacgcat
     1501 tgatacggac gcgcctttga aacccctgta cgtcatccac ggcgaggaag aactgttgcg
     1561 tatcgaggca ttggacgcat tgagggcggc ggcgaagaaa caaggttacc ttaatcggga
     1621 agtttatacg gcagacaatg ccttcgattg gaacgagctg ctgcaaaccg caggcagtgc
     1681 gggtctgttt gccgatttga agctgttgga actgcatatc cctaacggca agcccggcaa
     1741 aaccggcggc gaggcgttgc aggattttgc cgcccgattg ccggaagata cggtaacgct
     1801 ggttttgctg cccaaactgg agaaaaccca gctccagtcc aaatggtttg ccgcattggc
     1861 ggcaaagggg gaagtgtggg aagccaaacc ggtcggcgcg gcggctttgc cccaatggat
     1921 acgcggacgg ctggacaaaa tcggtttggg tatcgaggca gacgcattgg cactgtttgc
     1981 tgagcgcgtg gaaggcaatc tgttggcggc gcgtcaggaa atcgacaagc tcgggctgct
     2041 gtatccgaaa gggcataccg tcaatatcga tgaggcgcaa accgccgttg ccaacgtcgc
     2101 ccgcttcgac gcgttccaac tggcaggcgc gtggatgaag ggcgatgtcc tgcgcgtatg
     2161 caggcttttg gacggattgc gggaagaggg cgaagaaccg gtgctgttgc tgtgggcggt
     2221 tgccgaagac gtgcggacgc tgatccggct tgctgccgcc ctgaagcagg ggcagagcat
     2281 ccaatccgtc cgcaacagcc tcaggctttg gggcgacaag cagacgctcg caccgcttgc
     2341 ggtcaagcgg atttccgtcg tccgcctgct tgacgcgctc aaaacctgcg cccaaatcga
     2401 ccgaatcatc aaaggtgcgg aagacggcga cgcatggacg gtattcaaac ggcttgtcgt
     2461 gtcgctggcg gaataaagcg gtaatcccca aaatccgaaa atactgtaaa ataccgttaa
     2521 tcctgaaaag tattcaccaa tccgtccgaa aacatttcag acggcacgac cacctcaata
     2581 aaggaacatt aaccctatgg acaataagac caaactgcgc ttgggcggcc tgattttact
     2641 gaccaccgcc gttttaagcc tcattatcgt attgattgtc gattcctggc cgcttgccat
     2701 cctgcttgca gccgtcattg tcgctgccgc tgcgggcggt tttgtttgga catcccgccg
     2761 acagcaacgc cagtttatcg aacgcctgaa aaaattcgac atcgatcccg aaaaaggcag
     2821 aatcaacgag gcaaacctgc gccgtatgta ccacagcggc ggacaacacc agaaagatgc
     2881 gattaccctg atctgcctgt cgcaaaaatg ttcggtggac gaggcgcacg ctatgttcaa
     2941 aaaacgcccg acacgtcagg aaatcaatca aatggcggca aaacagtcgc gcggtcagaa
     3001 acgtccgcac cgttaaccgc cgcaaggcat ctttgcataa atgccgtctg aagcctgttg
     3061 gcgtttcaga cggcatattc tgattgaaaa gatgatgaca ctgaaaaccg ccccgctcaa
     3121 acgccgcttt gccgccatgc tgtacgaaat gctgctggtc ggtgcggcaa cctgtttggc
     3181 agcattgatt gccggtattg ccgccatttt tctgaatccc gtttctatcg cggtttctgc
     3241 attggtaaca agtatcctga taatgggagc atggtggctt tatttccgcg ccaactggca
     3301 tggtcagggg cagaccttgg cgatgaggac atggaaaatc ggcttgtgcg accttaacgg
     3361 catacagccg tctttgcacc tgctgcgcct gcgctttatt tgggcgtgca tatttatcgt
     3421 atttatccct atgttagcct atgccggatt acgccacttc ctcggcattc cgcccaaggg
     3481 cgcggccggc gcggcattga tttggctgat tttaccgtgg gggttcgcac tgctgaatcc
     3541 cgatcggcag tttctgtatg attttcttgc aggaacaaga ttggtggcgg tcaaaggaaa
     3601 gccttaagcc tttataccgc aaaggtttca acctgaaaaa atgccgtctg aaagggcttt
     3661 cagacggaat ttgcttatcg gggaaaccga ttattcgata ttctgcactt gttcccgcat
     3721 ctgctcgatt aagactttca gttcgaccga ggcttgggtg cattcggcgg caatggattt
     3781 gctgcccaaa gtgttggctt cgcggtttaa ttcctgcatc aggaagtcca gccgtttgcc
     3841 gctgctgcct ttgtgttcgg taacgatacg gcgcacttcg gcaatgtggg tgcgtaggcg
     3901 gctgaactct tcgtcgatgt cggatttttg gataaagagg gcaaattcct gttgcaggcg
     3961 gtcgttgtcg atgctgccga ccgcttcgac gaggcgggcg cggatttttt ctttatgtgt
     4021 ttccaacagg gtaggaaaga gttcgcttaa tgcatctatg atttcttcca tagcctcaag
     4081 gcgttgcagc aggtgctcgc ctaatttctt accttcccgc ttgcgtgcgg cagtaaagtc
     4141 ttttaacgct ttttcggtca gttcggtaat gctttttgcc aattcttccg tattttccct
     4201 ttggcttgcc aatacgccgg ggaaacgcag gatgtcggca acgcccagtt ttgccaaatc
     4261 gtgatgcttg cggaggtctt tgttgatttc ggcaagctgt ccgaccaagt cgcgattcag
     4321 ttccaaggac tgactgccgt tttccgcatc ttgaatttgg attttgcatt cgactttgcc
     4381 gcgtgcgata tgggatgaaa ttttctcgcg gataccgctt tccaaatagc gcaaatcgtc
     4441 gggcatcctg atttgaatat ctagaaaacg gtggttgacc gcacgcaggt cgagattgat
     4501 gcgtttgctg ccgcactctg ccgccgcgtt ggcaaatccg gtcatgctgt ggatgtggat
     4561 atttccgctg ctcatgtcgt tctccgaagc ccgttaaaat ggaatcaata tatcacatct
     4621 gtatggcggc aagcgttttc gggtgtgaaa aattgaagat tttgcagcgg cagattggaa
     4681 tcacgcgctt ttgttgctgc aaggaaggga aatgtatagt ggattaac
//
LOCUS       DAWGRZ010000154         4682 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000154 DAWGRZ010000000
VERSION     DAWGRZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.154"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..162
                     /locus_tag="WHT44_001720"
     CDS             <1..162
                     /locus_tag="WHT44_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
                     /protein_id="HEZ2533873.1"
                     /translation="DIWLHNGCLKISPSRHVKLEAWNAIDADDVILSLDNSPEEIGAG
                     LKLALSHCR"
     gene            175..362
                     /locus_tag="WHT44_001721"
                     /pseudo
     CDS             175..362
                     /locus_tag="WHT44_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222085.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(444..824)
                     /locus_tag="WHT44_001722"
     CDS             complement(444..824)
                     /locus_tag="WHT44_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ2533874.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPPVQNQAGTDDF
                     RAFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            1301..3382
                     /locus_tag="WHT44_001723"
     CDS             1301..3382
                     /locus_tag="WHT44_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ2533875.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPDRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEILAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            3372..3566
                     /locus_tag="WHT44_001724"
     CDS             3372..3566
                     /locus_tag="WHT44_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ2533876.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(3762..3953)
                     /locus_tag="WHT44_001725"
     CDS             complement(3762..3953)
                     /locus_tag="WHT44_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533877.1"
                     /translation="MDFQFFVETIRRQEKKLKHGVYRLFEIYSWLEDGKATKARIRYF
                     VKIEVDKQILRRIHRGIAD"
     gene            4082..4642
                     /locus_tag="WHT44_001726"
     CDS             4082..4642
                     /locus_tag="WHT44_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HEZ2533878.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINLPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT         1109 a         1343 c         1146 g         1084 t
ORIGIN      
        1 gatatttggc tgcacaacgg ctgcctgaaa atcagcccga gccgccatgt caagctggaa
       61 gcgtggaatg ccattgatgc agacgatgtc attttatcat tggataacag ccctgaagaa
      121 atcggagcag gtttaaagtt ggcattgagc cactgccgat aatatttgac aaaaggccgt
      181 ctgaaaaaca gctttgacaa agacgcggtt gccaaagaga tcgacctaca aagggaagta
      241 acgcaggcgt tcggcaaaac gccgcccaag ccgcagcggc cgttgccgac gaactcggca
      301 atacccgaag ttacggacgg tatcaggcag cccgaaccct gccggaggcc gaactgcaaa
      361 acaaatccga cacaaaaaac cgtccgtccg acacaaaaaa ccgcccgaaa aatctggacg
      421 gcggttcaaa caggctgccc cgtttaacgg gcgcggcagg aagtttcgac cgaattgccg
      481 taggcatcgg taaagccgaa aaaggcttcg ccgcctttct ggtgccactc ggttgcgttt
      541 ccgaacaaac cgtgttcggc ggtatagcgt tcgccggatg cggcaacgtc ggaagagagg
      601 acggcacgcc tgccgtccag ccgcaacgcg actttgccgc tgtccaaatg gcggacgcgc
      661 acagacaaac cgttctcgca ggaaaacgcc cgaaaatcgt ccgtgccggc ttggttttga
      721 acgggcggca tatgcccgcg tccgccgtca tcatacgcct ccggcacggc acaggccgcc
      781 aaagacaaaa ccggtacggt cagcgcgaaa aacctgatat tcataaaagc tccccaataa
      841 aaataagata tgaaacaacc gccctgattc caagctgcgg caacgccata ctataaacgg
      901 acgcgcaaac acacaagccc gataaccgga atttacctgc gatgaatcaa taatccggat
      961 tgcgcgcccc ttctttaccc ctcttccgat gccgcctttt gcctgacgat gccgtctgaa
     1021 cctgcctgcc cgcccggagg aatgtaaatt ttttccaaat tccaagtaaa aaccgctatc
     1081 ggtgtgctaa tttgcgttaa aatcctattc ggcgtttaac gttttgtgcg cccgcatccc
     1141 tgcactgttt gatgcgggca taaggcacaa atcccgacaa gcgcactgtt tcatacttcg
     1201 tcaatcattc agactccggt ttgtgcccgt gccggcagat ggttcggccg tttcccgccg
     1261 ttcaggcata ttccgacagt gtgagataag gatttattcg atgaaatcac tcaaaacctt
     1321 cctcatttgg ggcatagtgg tactggtcgg cttagcatcc tttaccactc tggccctcag
     1381 ccgaggcgaa caggtcagcg cggtatggat ggtcaccgcc gccatatccg tttactgcat
     1441 cgcctaccgt ttttacagcc tctacatcgc caaccgcgta atgcggctcg atcctgaccg
     1501 cctgactccg gcagaacgcc acaacgacgg cttggactac gttccgacgc acaaaggcgt
     1561 attgttcgga caccactttg ccgcaattgc cggcgcgggc cctttggttg gtccggtttt
     1621 ggcggcgcaa atgggttatc tgcccggtac tttgtggatt atcttcggcg tggtatttgc
     1681 cggcgcggta caggatatga tggtcttgtt cgtctctatg cgccgcgacg gtaagtcttt
     1741 gggcgatatt gtgaaacagg aactcggcac tgtccccggc gtgattgcct ccatcggtat
     1801 tttgatgatt atggtcatca ttatggcggt gttggcgttg attgtcgtaa aagcattggt
     1861 tcacagccct tggggtacgt tcaccattgc agcaactatg ccgattgcgc tgtttatggg
     1921 tatttacacg cgttatatcc gtccgggcaa aatcggcgag atttccatcg tcggctttat
     1981 tttgctgatg ctggcggtaa tttacggcga agatgtggct aaaagttcca tcgggcattg
     2041 gttcgacctt gacggcatcc agctcacttg ggcgattatg atttacggct ttgtcgcctc
     2101 cgtattgccc gtatggttgc tgctcactcc gcgcgactat ctctccacct tcctgaaaat
     2161 cggtacgatt gcggccttgg ctttgggtat cgtcatcgtc aatcccgctt tgcaaatgcc
     2221 tgccgtaacc cactttatcg acggttcggg tccggtattc tcaggcgcat tgttcccatt
     2281 cttgttcatt accatcgcct gcggtgcggt ttcgggcttc cacgcgctga tttcttccgg
     2341 cactacgccg aaaatgctgg aaaacgaaac ccacgtccgc atgatcggtt acggcggtat
     2401 gttgatggaa agtttcgtag ccattatggc acttgccgct gccgcatcgc ttgatcccgg
     2461 cgtgtacttc gccatgaaca gcccagccgc cctgatcggt acggatgcca ataccgccgc
     2521 cgaagtgatt accaccaagc tgcaattccc tgtcgatgcc gcaaccctgt tgcacactgc
     2581 taaagaagtc ggcgaaaaca ccatcctttc ccgtgccggc ggtgcgccca ccctcgcagt
     2641 cggtatggcg cacattatga gccgcctgat tccgggcgag gcgatgatgg cgttctggta
     2701 tcacttcgcc ctgttgtttg aagccttgtt catcctgacc gccgtcgatg ccggtacgcg
     2761 cgtcgcacgt tttatgattc aagacttggg cagcatcttc tacaaacctt tcggcaacac
     2821 cgactccatc cccgccaacc tgattgcgac cttcttcgcc gtggcattgt ggggctactt
     2881 cctctacacg ggcgtgaccg acccgttggg cggcatcaac tcgctctggc ctttgttcgg
     2941 catcgccaac caaatgctgg caggcgtagc cttgattatg tgcgccgtgg tgctgattaa
     3001 gatgaaacgc gaccgttatg tctgggtggt actcgttccc gccgtcggcg tactgttcgt
     3061 aacctgctac gccggcctgc aaaaactgtt ccacagcgac ccgcgcatca gcttccttgc
     3121 ccacgccggc aaatacagcg acgcattggc taaaaacgaa atccttgcgc ctgccaaaga
     3181 catcggcgaa atggcgcaaa tcatcttcaa cgacaagatt aatgccggtc tgaccatcct
     3241 cttcttgtcg gttgtcgtga ttgtcgccgc gtacggtttg cgtaccgccc tcaaagcacg
     3301 caaagtcggc tggccgaccg ccaaagaaat cccggcggtg taccgcgacg gcaaacagcc
     3361 ggaggcacaa agtgaagcat aagctcgcgt cttggtggaa aaccatcaag ctgacggcaa
     3421 acttgatggc aggcgtgccc gattatgaaa actacgttgc acagcagcgc aaacataatc
     3481 ccaacgcccc cgtgatgacc aagctgcagt ttcaagacta ttgccgcaaa cgccgctgcg
     3541 gcgcaaacgg cggacgctgc tgttaagcct gcttgaaaca aattccgtct gaacgccgct
     3601 tcagacggaa tttttataat atagtggatt aacaaaaatc aggacaaggc gacgaagccg
     3661 cagacagtac aaatagtacg aaaccgactc acttggtgct tcagcacctt agagaatcgt
     3721 tctctttgag ctaaggcgag acaacgccgt actggttttt gttaatccgc tataccacga
     3781 tgaatccttc gcaatatctg tttatcgacc tcaattttga caaaataccg gatacgcgcc
     3841 tttgttgctt ttccatcttc caaccaactg taaatctcaa acagccggta cacgccatgc
     3901 ttcagtttct tttcctgtcg gcggattgtt tcgacaaaga attgaaaatc catttcatgc
     3961 accttaaaat ttaatctgca ttcaaacctt ttcactttgg aagcaccatt tatcggatgt
     4021 cccttcgcaa taaacaaatt ttcccgatac cgccgcccat ttcaacccaa acccaaaagc
     4081 tatgaaaaac ctcatcgcct tcaacaaacc ctatggcgtt atctgccaat tttcaccgca
     4141 cgaaaaacac aaaagcctca aagactttat caatcttccc ggcttctacc ccgccggacg
     4201 gctcgacacc gacagcgagg ggctgctgct gctgaccgac gacggcaggc ttcaggcaca
     4261 aattaccgac cccaaattca aacaccctaa aacctactgg gcgcaactgg agggcgtacc
     4321 cgacgaaagc cgattggaaa gcctaagaaa agggatagac ttaggcggtt tcgttacccg
     4381 tccggcaagc atccgcatct tgaaacacgg agaagcagat tcgttatggg agcgcatccc
     4441 gccgatacgc gtccgcaaaa ccgttcccga tttttggatt gaaattacca tttctgaggg
     4501 caaaaaccgc caagtcaggc gaatgaccgc caaggcgggc tatccctgcc tgcgtctgat
     4561 cagagtggca agcggcaggc tgaaactgtt tgatttggat ttaaaacccg gggaatgggc
     4621 atacgccccg tttaaaccat aatcacgttt atctcatcat ttccacaaaa gtgggaatcc
     4681 gg
//
LOCUS       DAWGRZ010000155         4681 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000155 DAWGRZ010000000
VERSION     DAWGRZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.155"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            113..1504
                     /locus_tag="WHT44_001727"
     CDS             113..1504
                     /locus_tag="WHT44_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ2533879.1"
                     /translation="MSGQLGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            complement(1566..2318)
                     /gene="rlmB"
                     /locus_tag="WHT44_001728"
     CDS             complement(1566..2318)
                     /gene="rlmB"
                     /locus_tag="WHT44_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ2533880.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYIQEGKSDARTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMSGESDLYHCNLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKV"
     gene            complement(2385..2873)
                     /locus_tag="WHT44_001729"
     CDS             complement(2385..2873)
                     /locus_tag="WHT44_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ2533881.1"
                     /translation="MTVLTVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIERRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            complement(2878..3597)
                     /locus_tag="WHT44_001730"
     CDS             complement(2878..3597)
                     /locus_tag="WHT44_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEZ2533882.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLDGTPPQEMSQAHKARLLAELKNHPPVAAFPPQEEMEHFMCEALRQ
                     AEQSSADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGREIQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            complement(3601..4542)
                     /gene="miaA"
                     /locus_tag="WHT44_001731"
     CDS             complement(3601..4542)
                     /gene="miaA"
                     /locus_tag="WHT44_001731"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ2533883.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIIPPTESYSAARFVEDCTRLVGEISSRGRFALIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSTHLGSLTSHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTAYSPAIRCVGYRQAWKYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDCTSCTRLIEAAKRFFGA"
BASE COUNT          876 a         1119 c         1512 g         1174 t
ORIGIN      
        1 gtggtaaacg tgtggaccag acggatgccg tctgaaatgc aaattgaagc cgtgcggcag
       61 attcgctaca atccgcgctt ggatttttca acctttaaaa taaggaaata caatgagcgg
      121 tcagttgggc aaaggtgcgg atgcgcctga tttggtgtac ggtttggaag acaggccgcc
      181 gttcggtaat gcgctcttga gcgcggttac ccatcttttg gcgatttttg tgccgatgat
      241 tacgcccgcg ctgattgtgg gcggcgcgct ggaattgccg gtggagatga cggcgtatct
      301 cgtgtcgatg gcgatggttg cgtcgggtgt cggcacttat ttgcaggtca accgcttcgg
      361 gccggtcggt tcggggatgc tgtccatcca gtcggtgaat ttttcgttcg ttaccgtgat
      421 gattgcgctg ggcgcgggga tgaaagaggg cggtttgact aaggatgcga tgatttcgac
      481 gctcttgggc gtatcgtttg tcggcgcgtt tttggtgtgt ttctcggcgt ggcttctgcc
      541 gtatttgaaa aaagtgatta cgccgacggt cagcggcgtg gtcgtgatgc tcattggttt
      601 gagtttggta cacgtcggca ttaccgattt cggcggcggc ttcggcgcga aggcggacgg
      661 cacgttcggc tcgatggaaa acttggggct ggcatcgctg gtgttgctga ttgtgttggt
      721 gttcaactgc atgaaaaacc cgctgttgcg catgagcggc attgcggtcg ggctgattgc
      781 cggctatatc gtcgcgctgt ttttgggcaa ggtggatttt tccgcgctgc aaaacctgcc
      841 gctggttacg ctgcccgtac cgtttaaata cggttttgct ttcgactggc acgcgtttat
      901 tgtggcgggc gcgattttct tgttgagcgt gtttgaggcg gtcggcgatt taaccgcgac
      961 ggcaatggtg tccgaccagc cgattgaagg cgaggaatac accaaacgcc tgcgcggcgg
     1021 cgtgttggct gacggcttgg tgtcggtgat tgcgacggct ttgggttcgc tgccgctgac
     1081 gacgtttgcg caaaacaacg gcgtgattca gatgaccggc gtggcttcgc gccatgtggg
     1141 caaatatatt gccgtgattt tggtgctgtt gggtctgttc cccgttgtcg gtcgcgcgtt
     1201 tacgacgatt ccgagtccgg tgttgggcgg cgcgatggtt ttgatgttcg gcttaattgc
     1261 gattgcgggc gtgcggattt tggtcagtca cggcatccgc aggcgcgaag cggtgattgc
     1321 ggcaacgtcg gtcggtttgg gcttgggtgt cgcgtttgag ccggaagtgt ttaaaaacct
     1381 gcccgtcttg ttccaaaact ctatttccgc cggcggcatt acggcagtct tgctgaattt
     1441 ggtcttgccc gaagataaaa ccgaggcggc ggtcaagttt gataccgacc acttggaaca
     1501 ctgattttga aaatgaatgc cgtctgaaac agaatccctg tttcagacgg cattgttttt
     1561 gaggcttata ctttttcgtt ttttaatacg cgttgtcggc gtgtttcact taataccatt
     1621 ccggcagaca cggagacgtt catgctttcg actgtgccga acatgggtat agacaccagc
     1681 atgtcgcaat gttcgcgcgt gaggcggcgc ataccgtcgc cttcgttgcc cattacccac
     1741 gccgcgctgt cgggcagatt gcaatggtaa aggtcggact cgccgctcat atcggtgccg
     1801 ataatccaaa tgccgtattc tttcaattcg cgcagggtgc gggcgaggtt ggttacggtg
     1861 atatagggga cggtttccgc cgcaccgcag gcgactttgc tgacggtggc gttcagcccc
     1921 gcgcttttgt ctttcggtgc gatgacggcg tgtacgccca ttgcgtcggc ggtacgcagg
     1981 cacgcgccga ggttgtgcgg atcggtgatg ccgtcgagta tcagcagcag cggcggttcg
     2041 ctgaggtttt ccaatacgtc ttcgaggtgg acgtggtttt tggaggcatc gataaatccg
     2101 accacgccct gatggcgcgc gcctttgctg atggcgttga ggcggtcggc atcggcaaaa
     2161 tatacgcgga tgttttcgtt tgccgccttt tccaacactt cgcgcgtgcg tgcgtctgat
     2221 ttgccttctt ggatgtagag ttcgacgatg gatttggggt tttgccacaa tcgggcgttg
     2281 acggcgtgga agccgtagat gggtctttgg tttgccatga tggtgctttg taaaaagggt
     2341 tcagacagca ttatagcaat ttgccggtat gccgtctgaa agggttaaaa caggtaggcg
     2401 atgtatttca ccaacaggat aaacaagatg gatacggcgc agccgatttt gaacgccgtg
     2461 ccgacgacaa gccccaacag cgtacccaag cccgctttac ctgcctgaag catattgcgc
     2521 cgttcgatca gttcgcctgc cgccgcgccg ataaagggac cgagtattag tccgggaagg
     2581 gagaaaaata tgccgatgat gctgccggcc aatgcgccgc gaacggcgag cttgcccgct
     2641 ccggtatatt ttgtccccca tatgcctgcc acatagtccg ccagtatgcc ggcaaggctg
     2701 atgagtccga ccgtccacaa aacgcccgcg ccgtagattt ggtagccgcc ggcataggca
     2761 agcagccatg ttccggcaaa catcaatgcc aatccgggca gggcggggta aacgatgccc
     2821 gccgtgccga cggctatcag ggcgagggcg aggatgacgg tcagtacggt cataggttca
     2881 accttttctt ttgttttgaa aaaaacggct taacacggcg cggcattctt cttgcaggat
     2941 tccgccccgt atggcggtgt gcgtattgag gcgtttgtcg gcaaacaggt tgacgatgct
     3001 gcctgccgcg ccggttttgg gttctgccgc cccgtagatc acacgcctga ttcgtgcctg
     3061 tatcagtgcg gacgcgcaca tggcgcaggg ttcgagggtg atatatatgt cgcatccgtc
     3121 aaggcggtag ttttgtattt ctctgcctgc ctgtgccaag gcgttgattt cggcgtgtcg
     3181 gctgacattg cagtcggcaa tgcaggtgtt gtgtgccgat gcgatgattt tgccgtctga
     3241 aacgatgact gccccgacgg gtatttcgcc gtcggcggag gattgttctg cttggcgcag
     3301 tgcttcgcac atgaagtgtt ccatttcttc ctgcggcgga aaggcggcga cgggcggatg
     3361 gttttttaac tcggcaagca ggcgggcttt atgcgcttgg gacatttctt gcggcggcgt
     3421 gccgtccagc agcgactcga gttgccacag tgtgcttttc gtgagggtca aacccgatgc
     3481 tttgagcagc agaaaggctt tgaccgaacc gttttgccgc agttcttcga gtgtacggat
     3541 accgagcctg tgcagggcgg cgacggtttt gggggcgagc ggcggtgtgg tcagcatggt
     3601 ttatgcgccg aaaaaccgtt ttgccgcctc aatcaggcgt gtgcatgaag tgcagtctga
     3661 aaacgggtcg gcaacgcagt ctaaaggtgt tttgcgcaac caagtcagtt ggcgtttggc
     3721 aagttggcgg gtggcggcaa tgcctttctc gacaaatgcc gggaaatcgg tttttccatc
     3781 cagatatttc catgcctgac ggtagccgac gcagcggatg gcgggggagt aggcggtcag
     3841 gccgggatag cggcggcgca ggttttctac ttcgccgata aagccctgtt caagcatcag
     3901 gtggaaacgc agggcgatgt tttcatgcag gcgggcacgg ttttcgggaa tcagggcggc
     3961 ggtatgcaaa tcaaaaggga gcgtatggga ggtcaggctg ccgagatgtg tgctcatcgg
     4021 tttgccggtt aaataataaa cttccaaagc gcgtccgata cgctggctgt cgttcggttt
     4081 cagacggcat gcggtttcag ggtcgacttt ttgcagggtg cggtagagga aatccaagcc
     4141 gtacatctgt ttttgttcgt ccaagtcggc acgcaggcag gcgtcggctt cgggcaaatc
     4201 gttcaaacct tgggtcaggg cgcggaaata catcatcgtg ccgccgacaa taagggcaaa
     4261 cctgccgcgt gaggaaattt ccccgaccaa gcgcgtgcag tcttcgacaa agcgggcggc
     4321 gctgtatgat tcggtaggcg ggatgatgtc gataaggtgg tgcgggacaa aggcgcgttc
     4381 ggaggcggac ggtttcgccg tgccgatgtc catatcgcgg taaaccagcg cggaatcgag
     4441 gctgatgatt tcgacaggca gggtttcggc aattttgagg gcgagcgcgg ttttgcctcc
     4501 ggcggtcggc ccgagcaggg caaaggcttt cggggtcggc ataacgtttc aggtttggaa
     4561 aaatacggat tatagcggaa agcgtgccga cgttatattt tggtttgcgg aagcacgccg
     4621 acggcaaggg ggcgtgttta ccgtatgcct ttatatagtg gattaacaaa aaccagtacg
     4681 g
//
LOCUS       DAWGRZ010000156         4611 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000156 DAWGRZ010000000
VERSION     DAWGRZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.156"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(67..291)
                     /locus_tag="WHT44_001732"
     CDS             complement(67..291)
                     /locus_tag="WHT44_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ2533884.1"
                     /translation="MDAVQELERRIVELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(292..1680)
                     /locus_tag="WHT44_001733"
     CDS             complement(292..1680)
                     /locus_tag="WHT44_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ2533885.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGMMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAVAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(1766..2587)
                     /gene="prmC"
                     /locus_tag="WHT44_001734"
     CDS             complement(1766..2587)
                     /gene="prmC"
                     /locus_tag="WHT44_001734"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ2533886.1"
                     /translation="MTFDKWLGLSKLPKNEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISPPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(2594..3037)
                     /locus_tag="WHT44_001735"
                     /pseudo
     CDS             complement(2594..3037)
                     /locus_tag="WHT44_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
     gene            complement(3102..4586)
                     /gene="tldD"
                     /locus_tag="WHT44_001736"
     CDS             complement(3102..4586)
                     /gene="tldD"
                     /locus_tag="WHT44_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ2533887.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVHRFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
BASE COUNT         1045 a         1403 c         1223 g          940 t
ORIGIN      
        1 ttaggtttgc atcggcggaa tattcaaaca cagccttttt taaggaaatc cggatacggc
       61 ggcgcatcaa taatgcggcg gaatctcgtc gcgcagggaa tacggctctt gcgcgtcggg
      121 attcctgtcc tgcatttttt gatacagcag cctcaactga gcctgctgca aatccagcgt
      181 ctgccgcaat tccgccacca tcgcgttcag gccggcgatt acgtcctcct gaagcgcgga
      241 ttggatttcc agttcgacaa tacggcgttc caactcttga accgcgtcca tttacagcac
      301 catcgcggca atccagccgg caatcagcag cgggatgttg tagtggatga aggtcgggat
      361 aacggaatcg cggatgtggt cgtgctgccc gtcggcgttc agccccatcg tcgggcccag
      421 cgtggaatcg gacgcaggcg aaccggcatc gcccaacgcc cccgccgtgc cgacaatggc
      481 gacggtggca agcggcgaaa aacccaaacc gacacacaaa ggcacataaa tcgcggcaat
      541 aatcggcaaa gtggaaaagg acgaaccgat gcccatcgtt accaaaagcc ccaccaccag
      601 catcgccaat gccgccatac ctttgctgtt gccgaatatc gccatactgc tttccaccag
      661 cggctgaata tgcccggtcg cattcatcac ggcggcaaaa ccctgcgcgg caatcataat
      721 gaagccgacc atcgccatca tcttgatacc ttcgccgaat acgtcgtttg ccttgtcgcg
      781 gttaatgacc cccaacatca taaatacggc gaaaccgagc atcgcgccca acaccagcga
      841 gtcttcatac atcaactgga tggcaaagca tacggcaatg gcgacggcgg cggccaggct
      901 gcggtaggcg gacggctgcg gacggtttgc cgcatcggcg ttgcccgccg tatcggcatt
      961 gttgctttgg tacaggcgcg gtttgcggta atggacaaac gccagcagga gtccggccag
     1021 cattcccaac gcgggaatcg ccattgccgc catcacgtta atgtttttca catcaagctg
     1081 cggcgcggcg gaatggatgt tgcccaacag gatttcgttc aaaaaaatcg cgccgaagcc
     1141 gtaaggcagg aacatataag tcgtaaccag cccgaaagtg atgacgcacg caatcaggcg
     1201 gcggtcgatt ttcaggcggt tgaacaccaa aagcagcggc ggaacaatca tcgggataaa
     1261 ggcaatgtgg atggggatga tgttctgact catcatgccc atcacaagga tgatggaaag
     1321 cagcagccat ttgaccgcgc cctcgcccga acgcacgctg tcgggcatac cgccccggtt
     1381 cagcttgcgg acgaccgcgc cggcaagctg ctgcggcagg ccggaatggg taatcgccat
     1441 tgcaaacgcg ccgagcatcg cataagaaag cgcaatcttc gcaccgcctt ccaaaccttt
     1501 gttgaacacg gggataatcc ccgcctgact gacctgtccc gccgcatcgg caatgttttg
     1561 cagcggcata cccgccaccg cgccgccgac aaacgcgccg accgtcaggc tcaataccac
     1621 gtgcacgcgc gacagcgaca gcaccagcat aacgattacg gcaactacga ctgcattcat
     1681 tgtttatcgc tccaaaccta taaatgttta catatcgaaa cacatcataa cccaataacg
     1741 ggaaacccgc caattttgca aacaattatt tcaaatgctt catatacttc cccagcgtaa
     1801 ccctgtccaa acccgccaaa tccggcaggg tttccactcc tgaaaaacca ttctccgcca
     1861 acacgccgcg caccgccgcg ccctgatcga aaccgtgttc cagcaataaa aaaccgcctt
     1921 ccgccaaacg gtcgggcgcg ccttgcgcca aggtgcggat gcagcttagg ccgtctgaaa
     1981 agtcggtcag cgcgatttgc ggctcaaacc gcaaatcgcc ttgcaacaaa tgtttatcgc
     2041 cgttttcgat atagggcggg ttggacacga tgatgtccca tttcccttca gacggcatat
     2101 cggtgtcgaa ccacgaaccg tgtgcaaatt cgacccgcgc gcccaaatcc gccgcatttt
     2161 tccgcgccgt ttcaagggcg ggcgggctga tgtcggatgc gcgcacaaac gcatcggggc
     2221 gttcgagcgc gacggttacg gcaaccgcgc cgctgcccgt ccccaaatcc cacacgcgcc
     2281 cgttttcggg caggcgcgcc aatacggctt cgaccaaatg ttcggtttcg gggcgcggaa
     2341 tcagcacgct cggattgact gtaaagcgtc tgccataaaa ttcgcgcaca cctaaaatat
     2401 aggcaaccgg ctcgccgttc agacggcgtt gcgccagcct gtccgcccgc tgtcggactt
     2461 cgtccggcat ttcttccccg ccccgcgtca acaactgcac gcgcgtatat tccgaaacat
     2521 attgcagcag cattcttgct tcatttttag gcagttttga caagcccaac catttatcaa
     2581 acgtcatttt tatcccgtct gccgctgatg cggcttttct ttccttattc tttccggcaa
     2641 acgtaccgat ggtggcaacc gcaaatgcgg cataccacaa ataaaatcct gcaccgtagc
     2701 gcacaatatc cgatgtattc cctgcttcat cgacgtatac ggctttcaca ctgaaagcca
     2761 ccaacgccaa gccccaaagt gccgcatgga caggcacgac cttcttccgc aacgccagca
     2821 aaacaatggc cgccaaccaa acataattcg catagaccgc acaatacctg atatccaaag
     2881 aagcaaatat cgaccccaaa atcaaaacgg tcaaaccctc catgcttcca tgattgccca
     2941 aataaaatgc aacattggat aaagacgcta tccacagggc aaccgacacc agcaacatca
     3001 ctatgggaaa acttggtttc cgattctgtt cctgcatggt tttatcctaa tgtaaaaggc
     3061 cgcctgaaaa cctttcagac ggcatcgtgc cggattccgc gtcagattgc gctgccgccg
     3121 acggtcagtc cggcatcaat ccgcaaagtc ggttgcccca cgccgacggg gacgctctgc
     3181 ccttctttgc cgcacacgcc gacaccgctg tcgagcgcag tatcgttgcc tatcatggaa
     3241 acgtgtttca gcacttcggg gccgttgccg atgatggtcg cgcctttgac gggatattgc
     3301 agcctgccgc cttccaccca ccacgcttcg gacgcactga acacgaactt gccgctggta
     3361 atgtccactt gtccgccgcc aaagttgacg gcgtaaatgc ccttgtcgat ggacgcgatg
     3421 atttcttccg gctcatagct gccgttttcc ataaaggtat tggtcatgcg cggcataggg
     3481 gcggaagcgt aactttcgcg gcggccgttg ccggtggact gcgtacccgt caggcgggca
     3541 ttggtttcgt cctgcatata gccgactaaa atgccgtctt caatcaatac ggtgcggcgg
     3601 gtttcgttgc cttcgtcgtc gatgttgagc gaaccgcgcc ggccggcaat atcaccctga
     3661 tcgacgacgg taacgccttt ggcggcgacg cgctcgccta ttctgccgga aaagacgctg
     3721 gttcccttgc ggttgaaatc gccttccaaa ccgtgtccga ccgcttcgtg cagcaacacg
     3781 cccggccagc cgttgcccaa aacgacggtc atttcgccgg cgggcgcggg gcgggattcg
     3841 aggttggtga gtgcctgttt gacggcggca tcgacaaacc gatgaaccaa gttttcatcg
     3901 aaataagcca agtcgtagcg tccgccgccg cccgcgctgc cctgttcgcg gcgttcgccc
     3961 tgtttggcga taacggtaac gttcaggcgc accatcgggc ggatgtcggc ggcgtgtttg
     4021 ccgtccagac gggcgaggta aaccatatcg tattcgcagg tcaaaccggc catcacttgc
     4081 acgatgcgcg gatcggcggc tttggcgatt gcttccactt tgttcaacag cgcgactttg
     4141 gcggcggaat cgaggccggc aatggggtcg gacgcggaac aaaccggctt gccgcgcgtt
     4201 tcagacggca ttttggcgga caccttgccg cctgccgccc caatcgcgcg gacggcgcgg
     4261 gcggaacggt ttatcgaatc gatgcacagg ctgtcggcgt aggcaaaggc ggttttgtcg
     4321 cccgaaacgg cgcgcacgcc cacgccctga ttgatttgga agctgcccga tttgacgatg
     4381 ccctcttcca aatgccagct ttcataagcg gtgcgctggc agtagatgtc ggcgtaatcg
     4441 acgtggtgcg cgccgatgat gcacaggctt ttggcgagca gttcggggga aaggcggttg
     4501 gcttcgagca gccgcgcctg tacggcggaa taggtcggat gcatagtgtc ggcgcataaa
     4561 aaatcagggg cttgattata cggcatttgt tatatagtgg attaacaaaa a
//
LOCUS       DAWGRZ010000157         4577 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000157 DAWGRZ010000000
VERSION     DAWGRZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.157"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(279..386)
                     /locus_tag="WHT44_001737"
                     /pseudo
     CDS             complement(279..386)
                     /locus_tag="WHT44_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(432..1160)
                     /gene="pdxJ"
                     /locus_tag="WHT44_001738"
     CDS             complement(432..1160)
                     /gene="pdxJ"
                     /locus_tag="WHT44_001738"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ2533888.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAARDVGAPVVELHTGAYADAR
                     SHAEQIRQFERIQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(1184..1975)
                     /gene="recO"
                     /locus_tag="WHT44_001739"
     CDS             complement(1184..1975)
                     /gene="recO"
                     /locus_tag="WHT44_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ2533889.1"
                     /translation="MSEYRVNHEPVFMLASSPWRESSLWVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSVSWYGSQELKTLHRAEWVGGWRQPQGRALFGGLYVNELVLK
                     LTAREDPVPELYDALAEVMEAVCCKAAYIDDLRRFEWRLLNLLGVAPDLNRDGDGGTI
                     AAGGTYLVRPETAVFPVGKGFAVPPHAAGVVAPGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(2012..3139)
                     /gene="pheA"
                     /locus_tag="WHT44_001740"
     CDS             complement(2012..3139)
                     /gene="pheA"
                     /locus_tag="WHT44_001740"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ2533890.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDDCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEIVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(3178..4410)
                     /locus_tag="WHT44_001741"
     CDS             complement(3178..4410)
                     /locus_tag="WHT44_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ2533891.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLIVYCLAVAAIVFVSSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRL
                     KTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENGQSEYL"
BASE COUNT         1022 a         1313 c         1257 g          985 t
ORIGIN      
        1 gcttcgtcgc cttgtcctga tttttgttaa tccactatat tacataatca tcgcctcaat
       61 atttaatatc ccaaaatata gatttaaaac catcgccttt gtggtttgtc agcattccta
      121 attgagatag aaagtcaaat aatttcttgt ttctccggct gccatcaaga tggtttagat
      181 taagacctcg aacaatacaa tacccaatac tctgtactgt attgtgaatt tcttctatca
      241 aactattatt gtttgcagat aaaatatact tttgattcat atagtgaatt aaatttaaac
      301 cagtacggcg ttggcttgcc ttgccgtact atttgtactg tctgcggctt cgccgccttg
      361 tcctgattta aatttaatcc actataacat atccctgtcg tgaaatgctg tctgaaaggg
      421 tttgcctgcc cttacggcag cagccttgcc ctgaacatcg cctccttcat ttggcgcacg
      481 gcttcgggca gtccgaggaa gagggcttgg gcaatcagcg aatgcccgat gttcagttcg
      541 cggatggcga ggatttgggc gatgggggta acgttgtgta tggtcagtcc gtgtccggcg
      601 ttgacgacca agcccaaatc gccggcgaaa tgcgcgccgt tttggatgcg ctcgaactgc
      661 ctgatttgtt cggcgtggct gcgcgcgtcg gcatacgcgc ctgtgtgcag ctcgacaacg
      721 ggcgcgccga catcacgggc ggcttggatt tgcctgtcgt cggcatcgat aaacaaagac
      781 acgcgtatgc ctgcgtcggt caggattttg gtgaacccgg cgattttttc ctgttgcgcc
      841 aatacgtcca aaccgccttc ggtcgtgatt tcctgacgtt tttcaggcac gatgcacacg
      901 tcttccggca tcactttcaa agcgttttcc aacatttctt ccgtcaacgc catttcaagg
      961 ttcaggcgcg tgcggatggc gtttttgacg gcaaacacgt ccgcgtcttt gatgtggcgg
     1021 cggtcttcgc gcaggtgcat ggtaatcaaa tccgcaccgt gcgtttcggc aaccagtgcc
     1081 gcctccacgg ggctgggata agtcgtaccg cgcgcattgc ggacggtggc gatgtggtcg
     1141 atgttgacac ctaaaagcat aatctttcct tttatttctg ccttcagacg gcatttgaag
     1201 ccgtgccgtc cgaagtcggg acggtttccc gggcggtttc tttgcggtca aactgccgta
     1261 tctgttccaa cacctgccgc gatttcagcc cctcgggcaa caggtggcgg ataaaaagcc
     1321 gtgtgatttt caatgcctgt tgcaggcttt cggcagtgcg gaaactgcct tcgcgcaaat
     1381 cgatcaggct ctgcccgggg gcgacaacgc cggcggcgtg cggcggtacg gcaaatcctt
     1441 ttccgacggg gaagacggct gtttccgggc ggacaaggta tgtgccgcct gccgcaatcg
     1501 tcccgccgtc cccgtcgcgg ttcaaatcgg gggcaacgcc caacaggttc agcagccgcc
     1561 actcgaaacg gcgcaagtcg tcgatataag cggctttgca gcacaccgcc tccatcactt
     1621 ccgccaacgc gtcgtataac tcgggcaccg ggtcttcgcg ggcggtcagt ttcaacacca
     1681 actcgttcac atacaatccg ccgaacaacg ccctgccctg aggctgccgc caaccgccga
     1741 cccattcggc gcggtgtagg gttttgagtt cctgactgcc gtaccacgac acgctgacgg
     1801 gcacgaacgg caccaatacg ccgcgcagct cgctctgcct tttgcgcgcg ctgcgcgcca
     1861 gcaaagccac acgcccgtaa cggcggctga atgcttcaac ccacaggctg ctttcgcgcc
     1921 agggcgaaga tgccagcata aaaacgggtt catggttgac tcggtattcg gacataatcg
     1981 ttggggaaat gccgtctgaa cgctgccgcc gctacaaaac ggcggtcggg tacgaaccga
     2041 tgactttgac gaacgaagcg cgttcgccca agcgttccaa tgccgtctga atctgcgcgt
     2101 cccggcggtg tccttcgatg tcgatgaaga acaggtattc ccacaaaacg gatttgctcg
     2161 gacggctctc aaacttggtc atggaaatac ccgattcggt cagcggttgc agcagcgagg
     2221 caaccgcgcc tgcccggttg ggcgcggaaa cggccagcga agtcttgtcg ctgccgcttg
     2281 caccggtttc gtgatgtccc atcaccaaga agcgcgtggt gttgttcggt tcgtcttcga
     2341 tgcactcggc aaccatatcg agtccgtaga tttccgccgc cgtgcgtccg gcgatggcgg
     2401 caaccgtacc gtcgtccgat tcggcaacca gccttgcggc ttcggcattg ctggacacgg
     2461 caatccgttc ggcgttgggc aggtgtctgc ccaaccagtc gttgcactgc gccaacgcct
     2521 gcgcgtggga aaagactttg gcaatgcctt cggtgctgcc gttgttttta cgcaaaaggt
     2581 tgtggtggat gcgcaaaacg atttcgccgc acgcctgcaa cgcggtaacg gcaagcaggt
     2641 ctaacgtgcg accgaccgag ccttcggtcg aattttccac gggggcgacc agataatccg
     2701 cctgacgcgt ttcaacctgc ttgaagcagt cgtctatggt cggacacgcc atggtgtgcg
     2761 cggcgtgtcc gaaatgtttg attgccgcct gctgggtaaa cgtgccctgc ggccccagat
     2821 aggcgatggt cagcgggcgt tcgacggcga ggcactcgct catcacttcc cgaaacaggc
     2881 gtgctaccga ttcgtcgggc agcgggcctt tgttcaaatc ctgaatgcgg cgcaacacgg
     2941 cgacttcgcg ttcggggcgg tacactgcgc ccgtgccttt cagctcgccg atggcgtggg
     3001 cgtgttgcgc acgttcgttg agcaggcgca ggatttcggc atcgatggtg tcgatggcgt
     3061 tgcggtgggg aaggaggagt tcgtcgatag tttgggacat aatttcgcct gaacagtaat
     3121 ttatcgtgca ttctaacatc aaatgccgtc tgaaacggtt tcagacggca ttttccgtta
     3181 aaggtattcg ctttgcccgt tttctttcca cgcacgatgc gagcagagcc acagaagcgc
     3241 aatgccgcaa gaggtggacg cggtcatcgt tgccgccatc acggttgccg aaccgtcgtg
     3301 caagaaggtc gccgccatac ccacccccgc gccgattaaa gattggaata cacccaatac
     3361 ggcgtttgcg ctgccgccct cttctttgaa ataggacata aaacacgcct gcgtgtttgc
     3421 accgaccaag ccctgcgtac cgacggaaaa catcacgcac gcgaccagca gccaaaacgg
     3481 gggcaacccg aaaaacagca cggcggcgag ttgggacagg ttggcggcaa actggacgac
     3541 aatcccccac agcaggatgc tttgcggatg cacgccggtt ttgagccgcc acgcggtaac
     3601 gcggttgaaa aacatcatcg tgatgatgtt gagtgcaaac gcccaagcgt attgatgagg
     3661 cgtaacacgg tagagctgct ggtacacgaa ggaagattcg gtcagaaagg cgaacatcga
     3721 accgaagctg aatgcctgaa aaaacagata acccatcgca gcacgggttt tcaatacgcg
     3781 cttgaaccgc cccgccacca gcccgaacac gtcccgtccg attttgccgc cgacggcggg
     3841 cttgggcagg aaatactgta ccaaaccgag cagcaccagc gaatacgccg ccagaaaaac
     3901 aaaaatcgcc tgccagccac ccaagccctg caacaatgcg ccgaccatgg gtgcgaccag
     3961 cggcacaacc atcaaaatga tgccgataag ggcaaacatc tgggcggctt tgcgtccgga
     4021 ataataatcg cgcaccattg cgccgacgat gaccacagtc atgcccgcac cgaatgcctg
     4081 cacgacgcgc aggttgagga gctgttcggc actcgaaaca aatacgatgg cggcaacggc
     4141 aaggcaatat acaatcaaac cggtcagggc gacgggtttg cgccctttga tgtcggacac
     4201 cgaaccgccg accacctgtc cgaacgccgt gccgaacata aacaaactca aactctgttc
     4261 gatgcggtga acatccgcgt tcagcgattg cgccatttcg ggaatcgcgg gcaggtaggc
     4321 atcgatggaa aacggcatca gcgtaaccag catcgccatc aaaaccgcca tcagtttttc
     4381 gctcatttca ggataatggg cagaaggcat aaattctcgt ccgattcgca aacaaaccgc
     4441 ccggcggcgg aaaacacaat acgcggaaac ggaaaatttt aacacaccgg cacaaacagg
     4501 cggcaacaaa agccccggca aaaccggacg aaccggaacg atatagtgga ttaacaaaaa
     4561 ccagtacagc gttgcct
//
LOCUS       DAWGRZ010000158         4506 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000158 DAWGRZ010000000
VERSION     DAWGRZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.158"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(103..1041)
                     /locus_tag="WHT44_001742"
     CDS             complement(103..1041)
                     /locus_tag="WHT44_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ2533892.1"
                     /translation="MLELNGLCKRFGNKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NIIAGIVRPDGGEIWLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRGTLRRMTAERIRNGGIPAVLVTHSPEEACTTADEIAVMHKGRILQYGTPET
                     LVKTPSCVQVARLMGLPNTDDNRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMRHAGAVSGKDTVRIHIEEREIVRFR"
     gene            complement(1050..1925)
                     /locus_tag="WHT44_001743"
     CDS             complement(1050..1925)
                     /locus_tag="WHT44_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ2533893.1"
                     /translation="MPTVRFTESVSKQDLDALFEWAKASYGAESCWKTLYLNGLPLGN
                     LSPEWVERVKKDWEAGCSESSDGIFLNADGWPDMGGRLQHLALGWHCAGLLDGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGRRSPHKAVDPNK
                     LDNTAAGGVSGGEMPSEAVCRESSEEAGLDKTLLPLIRPVSQLHSLRSVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVAGFEKMDIGGLLDAMLSGNMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2121..2197)
                     /locus_tag="WHT44_001744"
     tRNA            complement(2121..2197)
                     /locus_tag="WHT44_001744"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2161..2163),aa:Met,seq:cat)
     regulatory      2347..2446
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2539..4440
                     /gene="thiC"
                     /locus_tag="WHT44_001745"
     CDS             2539..4440
                     /gene="thiC"
                     /locus_tag="WHT44_001745"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ2533894.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGICFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKPEYAKLLEQHAGQSFGANIPTHPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1090 a         1296 c         1187 g          933 t
ORIGIN      
        1 gtttcggata aaattttgca gccctgataa aaaaatatgg ctgctttggt aaaaaaatgc
       61 cgtctgaaag gttttcagac ggcattttgt ttttaagaag catcagcgga agcggacgat
      121 ttcccgttct tcgatatgga tgcgtaccgt atccttgccc gataccgccc cggcgtgccg
      181 catatcgagg ttcagccaca ggatgccgtg ttccggatgg aggacggaca ggctgaacga
      241 ttcgggcaaa caggtacggg ataatacgcg gcactccatg ccgtcttggt cgaaacgcac
      301 cgcatgttgc ggaatatggc ggttatcgtc ggtattgggc aaacccatca gtcgggcgac
      361 ctgcacgcag gatggtgttt tgaccaatgt ttcgggcgta ccgtattgta gaatcctccc
      421 tttatgcatc acggcgattt cgtctgccgt cgtacaggct tcttcgggcg aatgcgttac
      481 caaaacggca gggatgccgc cgtttcggat acgttcggca gtcatacggc gcagcgtgcc
      541 gcgcaaatgc gtgtccaaac tggaaaacga ttcgtccaac agcagcaggg aagggcggac
      601 aaccaaagcg cgcgccaacg ccagccgttg cttctcgcct ccggaaagtt tttcaggctt
      661 gcggtgcgcc tcgttttcca gtccgacttc ggcaagtgcc gccatggcga ggcgttcggc
      721 ttcggctttc ggcatttttt gcattttcaa accgaatgcc gcattttcca gcgcactcat
      781 atggggaaac agcgcgtaat cttgaaacat cagcgagata cggcgttttt cgggcggcat
      841 acgggtaatg ttttctccgt tcagccatat ttccccgccg tccggccgga caatccccgc
      901 aattatattc agcagggtgg attttccgca gcccgaccgc cccaaaacgg cgagtatttt
      961 gccgcgcccg acagtcaggc agatgttgtc ggcgacggtt ttattgccga agcgtttgca
     1021 gagtccgttc agttcaagca tggcgcatcc tataaacgta tgccgtccag ccactcggac
     1081 agcggatggg cggcatcaat cagaccgtaa cggcaaaacg cgtccagcgt aaccagttgc
     1141 gcgtcgtgca tcatgtttcc cgacaacatg gcatccaaca gaccgccgat gtccattttc
     1201 tcaaaacccg ccacttcgcc atcctgattt tcaggcagga aggtttcggg caggacggca
     1261 tcgaatacat acaggatttc attgtgtaca ccccggctga cggagcgcag gctgtgcagc
     1321 tgcgataccg ggcggatgag cggaagcagc gttttatcca aaccggcttc ttcgctgctt
     1381 tcgcgacaca cggcttcaga cggcatttcg ccgccggaaa caccgccggc ggcagtattg
     1441 tcgagtttgt tgggatcgac tgctttgtgc ggactgcgcc tgcctatcca gaaatgccat
     1501 cggccgtccg attcggtcag accgttgaga tggacggcgc ggctgagcag tccgaaagga
     1561 cggaaagcgg cgcgttcgag cgtgaacaag gggttgccgc cgccgtcggt caggtcgaaa
     1621 cactcgttgc gccagccgtc caacagcccc gcacagtgcc aaccgagggc gaggtgctgt
     1681 aagcgtccgc ccatatcagg ccagccgtcc gcattcagaa aaatgccgtc tgaagactcc
     1741 gagcagcctg cctcccagtc ttttttgacg cgttccaccc attccggcga caggttgccc
     1801 aaaggcagac cgttcagata cagcgttttc cagcaacttt ctgcaccgta acttgctttt
     1861 gcccactcga acagagcatc aaggtcttgt ttgctgacgg attcggtaaa acggacggtc
     1921 ggcatggttg gctttcttga agacaatgcg gaagattgta gccaaactgc ctgaatttgc
     1981 aaccgcaaac agacggcggt ctgccgttcc gatattgcgg ccggaaggaa aatccggcaa
     2041 aaaacggagg cggcaggaaa caggaaacag gaaacaggca aaacaaaacc cctcggcatt
     2101 gaaccgaggg gtttgatatt tggttgcggg agcaggattc gaacctacga ccttcgggtt
     2161 atgagcccga cgagctacca tgctgctcca tcccgcgtca gaagatgaaa ctatacggca
     2221 gattttttat gttgtcaaac attacttccg cgaaaaatca taaaattttg ccgtccggcg
     2281 gtttatgcct gatttgaaat attattttct ttacaaaagt tcatgttcgt gatttaattt
     2341 tggttaacat tgaaacaggg gtgctgcctg atgtttaggc ggctgagaaa taccctttac
     2401 acccgatcgg gataatacct gcgtggggag ttttcacgga ttctgctttt cagacggcat
     2461 tggttttcaa atgccgtctg aaaacgcaaa acgctcctgt ttctttaatt ctaaacgaga
     2521 aaacaggagc atttttttat gactacgcca aaaaaaaccg ccaaaacttc cggcaacgaa
     2581 gcgcgcgagc ttgccgactt gagcgaagac atcggcatct gctttaaata tccgaactcg
     2641 gaacgcgtgt atctgcaagg cagccgcgac gacatccgcg tgcctttgcg cgaaatccgt
     2701 caggacgaca cctacacggc gcaaggtacg gaagccaatc cgccgattcc cgtctacgac
     2761 accagcggcg tgtacggcga cccggcggcg catatcgacc tgaaacaagg tctgccgcac
     2821 atccgcaccg cgtggctgga cgaacgcggc gataccgaaa tcctgcccaa gctctccagc
     2881 gaatacggca tcgaacgcgc acacgatccg aaaaccgccc atctgcgttt caaccaaatc
     2941 acccgcccgc gccgcgcgaa aagcggcagc aacgtaaccc agcttcacta cgcgcgccaa
     3001 ggcattatca cgcccgaaat ggagtttgtc gccatacgcg aacgtttaaa attagacgaa
     3061 ttgtcccaaa aaccggaata cgccaaactc ttggaacagc acgcggggca aagtttcggt
     3121 gcgaacatcc cgacccatcc cgaccaaatc acgcccgaat tcgtgcgcca agaaatcgcc
     3181 gccggacgcg cgattattcc cgccaacatc aaccaccccg aactcgaacc gatgattatc
     3241 ggccgcaact ttcgtgtcaa aatcaacggc aacttgggca actccgccgt cacctccagc
     3301 ctgaccgaag aagtcgaaaa aatggtgtgg tcgctgcgtt ggggcgcgga cacgattatg
     3361 gatttgtcca ccggcgcgca catccatgaa acgcgcgaat ggattatccg caacgcgccc
     3421 gtccccatcg gcaccgtgcc gatttaccaa gcgttggaaa aaaccggcgg catcgccgaa
     3481 gatttgactt gggatttgtt ccgcgacacc ctcatcgaac aggcggagca aggcgtggac
     3541 tatttcacca tacacgcggg cgtgttgctg cgttatgtgc cgatgaccgc caaccgcctc
     3601 accggcatcg tctcgcgcgg cggttcgatt atggcgaaat ggtgtttggc acatcatcgg
     3661 gaaaatttcc tctacacgca tttcgacgaa atctgcgaaa ttatgaaagc gtatgacgta
     3721 tcgttcagcc tcggcgacgg cctgcgcccc ggctgcattg ccgatgccaa cgacgaatcc
     3781 caattcgccg aactgcacac cttgggcgaa ttgaccgata aagcgtggaa acatgacgta
     3841 caagtcatga tcgaaggccc cggccatgtg ccgctgcaac gcgtcaaaga aaacatgacc
     3901 gaagagctgc aacactgctt tgaagcacct ttttacacgc tcggcccgct cgttaccgac
     3961 atcgcacccg gctacgacca catcacctcg ggcataggcg cggccaatat cggctggtac
     4021 ggcacggcga tgctttgtta cgttaccccg aaagagcatt tggggctgcc cgacaaagaa
     4081 gacgtgcgca ccggcatcat cacctacaaa ctcgccgccc acgccgccga tctcgccaaa
     4141 ggctggccgg gcgcacaatt acgtgacaac gccctgagca aagcgcgttt cgagttccgc
     4201 tggcgcgacc aatttcgctt aagcctcgac cctgaacgtg ccgagagctt ccacgacgaa
     4261 accctgcctg ccgaaggcgc gaaaatcgcc cacttctgct cgatgtgcgg ccccaaattc
     4321 tgctcgatga aaatcacgca ggaagtgcgc gactacgccg acaagcaaaa agcccaacgg
     4381 cagggcatgg aggaaaaagc ggtcgagttc gtcaaaaaag gcgcgaagat ttacagttaa
     4441 acgtcaagca aaaaatgccg tctgaaaacc ggaaaaaagg cttcagacgg cattctttcg
     4501 cttgtg
//
LOCUS       DAWGRZ010000159         4501 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000159 DAWGRZ010000000
VERSION     DAWGRZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.159"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            26..2041
                     /locus_tag="WHT44_001746"
     CDS             26..2041
                     /locus_tag="WHT44_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ2533895.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLENDKARALS
                     DGILAQLQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLSKLGSDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWNERQLTQSGYPREVWLVERGKSFEESLPVYQIGEDGMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLRVSAEGEAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGINVQQFWTTSADGERIPYFHVGKNAAPDMPTLVYAYG
                     GFGIPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPEHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSAQSWLYSPDGGGHTGNGTQRESADELACVLLFLKEFLG"
     gene            complement(2156..3112)
                     /locus_tag="WHT44_001747"
                     /pseudo
     CDS             complement(2156..3112)
                     /locus_tag="WHT44_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            3348..4313
                     /locus_tag="WHT44_001748"
     CDS             3348..4313
                     /locus_tag="WHT44_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ2533896.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGARQLIQAAEQLKAAFKKAEPVAAGKK"
BASE COUNT          953 a         1224 c         1399 g          925 t
ORIGIN      
        1 acctgcccaa ccctcaagga ccccgatgaa atcctacccc gacccctacc gccattttga
       61 aaacctcgat tccgccgaaa cgcaaaactt cgctgctgaa gcgaatgccg aaacgcgcgc
      121 gcgtttttta gaaaacgaca aggcgcgcgc gctttcagac ggcattttgg cgcagttgca
      181 ggacacgcgg cagattccgt tttgtcagga acaccgcgcg cggatgtacc atttccatca
      241 ggacgcggag tatccgaagg gcgtgtaccg cgtgtgtacc gcggcgacgt atcgttccgg
      301 ctatcccgag tggaaaatcc tgttttcggt ggcggatttc gacgaattgc ttggcgacga
      361 tgtgtatttg ggcggcgtgt cgcacttggt ggaacagccc aaccgcgcgt tgttaacact
      421 gagcaaattg ggcagcgata cggcgtacac gctggaagtg gatttggaag caggggagtt
      481 ggtcgaaggc ggttttcact ttccggcagg caaaaaccat gtgtcgtggc gcgatgaaaa
      541 cagcgtgtgg gtgtgtccgg cttggaacga acgccagttg acccaatcgg gctatccgcg
      601 cgaagtatgg ctggtggaac gcggcaagag tttcgaggaa agcctgcctg tgtatcaaat
      661 cggcgaagac ggcatgatgg tgaacgcgtg gcgttatctc gatccgcagg gttcgccgat
      721 tgatttgatt gaagcgtcgg acggttttta caccaaaacc tatttgcggg tctcagccga
      781 aggcgaggcg aaaccgttaa acctgcccaa cgattgcgac gtggtcggct atctggcggg
      841 gcatcttttg ctgacgctgc gcaaggactg gaaccgcgcg aaccaaagct atccgagcgg
      901 cgcgctggtg gcggtgaagc tgaatcgggg cgaactcggg gcggcgcagc ttttgtttgc
      961 gcccgatgaa acgcaggcat tggaaagcgt ggaaacgacc aagcgttttg tggtggcgag
     1021 cctgttggag aacgtacaag gccgtctgaa agcatggcgg tttgccgacg gcaaatggca
     1081 ggaagtcgaa ttgccgcgcc tgccttcggg cgcgttggaa atgaccgacc aaccttgggg
     1141 cggcgacgtg gtttaccttg ccgccagcga tttcaccacg ccgctgacgc tgtttgcgct
     1201 ggatttgaac gtgatggaac tgaccgtcat gcgccgccag ccgcagcagt ttgattcaga
     1261 cggcattaac gtgcagcagt tttggacgac ttcggctgac ggcgagcgca ttccttattt
     1321 ccacgtcggc aaaaacgccg cgcccgacat gccgacgctg gtctatgcct acggcggttt
     1381 cggcattccc gaattgccgc attatctggg cagcattggc aaatattggc tggaagaggg
     1441 caatgccttt gtattggcga acatccgcgg cggcggcgag ttcggcccgc gctggcatca
     1501 ggcggcgcag ggaatcagca aacataaaag cgttgatgat ttattggcag tcgtgcgcga
     1561 tttgtccgaa cgcggtatca gttcgcccga acacatcggc ttgcagggcg gcagcaacgg
     1621 cggactgatt actgccgccg ccttcgtgcg cgaaccgcaa agcatcggcg cgctggtgtg
     1681 cgaagtgccg ctgaccgaca tgatccgtta tccgctgctc tccgccggtt caagctggac
     1741 agacgaatac ggcaatccgc aaaaatacga agtctgcaaa cgccggttgg gcgaattgtc
     1801 gccgtatcac aatctttcag acggcatcga ttatccgccc gcgctcatta ccaccagcct
     1861 gtccgacgat cgcgtccatc ccgcccacgc gctcaagttc tacgccaaac tgcgcgaaac
     1921 ctccgcgcaa tcttggctct actcgcctga cggcggcggc cataccggca acggcaccca
     1981 acgcgaatcc gccgacgaac tcgcctgcgt cttgctgttt ttgaaagagt ttttgggcta
     2041 agggcggggg agcggcactg ccgccgcgaa tgaaaaaagg tcgtctgaaa ctgctttttc
     2101 agacgacctt ttttaatggt tgtttcaaat caaaatatct atgccgccgg ccccatcagc
     2161 acttcttcac atccgaaggc aaaaatccgt aatgccgtct gaacgcttcg ttgaaccgtc
     2221 ccgcgtggcg gtagccgcaa aagtgcatgg cggcttggac ggtgctgccg gattcgatga
     2281 gggcgagcgc gtgttccagc cgcaggcggc gcaggcatcc ggcgacggtt tcgccggttt
     2341 gcgctttgaa atagcgtttc aggtagcatt cgttcagtcc gacgcggcgg gcgatttcgg
     2401 cgatggtcag cggacgggcg aattcgtgtt gcaggatgtc ggcggcttcg tctatgcgcc
     2461 gacggcggta accgttgtcg tggcggcgga aggtgaagcg caataatcgg gcggagagtt
     2521 ccagcgcggc ggcttcgtcg gcaagcaggc cgaagccgtc cgattcgaac gggcgttgca
     2581 gcagtgggca ggccgccgcc gtcagtgccg ctgcgttttg cgccagccgt tgcagggcga
     2641 atcggcctat tgtttgcggc gaaaacaggc gttcgtccag caagccttcg tcgtgccagc
     2701 ggcgcagttt ttccagcgaa aaatccaaat gcagcgcgca catgccgctg ttgtcgggca
     2761 gcagggtttc ggatacgtcc gccaaatcgc cgcgtaccag tcagatttcg ccggcagatg
     2821 ggcggtattc cctgccgccc atttgtaacc ggttctgccc cgacaccatg acgaacaagg
     2881 cgcagttgtg gctgaaattg tggatttcgg tgggaaacgc gcccgttccg ccgccgcgca
     2941 tccgcgacaa ggtgatgccc gaatcgaagc ggttgatgca catttccaga tgcaaaccgg
     3001 gctgttttgc ctgcgcaatg agcgcgctgt cggaacagcc gtccaacgcc cagccggatt
     3061 tatcggagcg gacataggtt tggtactggc ggtagatggc ggcggtgttc atgattggat
     3121 aggaacgagt tgtctaacaa atgaattaaa taggaattat taccaataat caagcgcagg
     3181 gattggttga aacggaaaag gtcgtctgaa agggtgtttc agacgacctt ttccgtatcg
     3241 ggaatttgtt ttgccgtatc gggaattttg cgttttgcgg cgtggtttct gcaggttgtt
     3301 tgcttaataa taaacattct tattcgtatg caaaggaacc gcacaccgtg aaaccgcgtt
     3361 tttattgggc agcctgcgcc gtcctgctga ccgcctgttc gcccgaacct gccgccgaaa
     3421 aaactgtatc cgccgcatcc gcatctgccg ccacgctgac cgtgccgacc gcgcggggcg
     3481 atgccgttgt gccgaagaat cccgaacgcg tcgccgtgta cgactgggcg gcgttggata
     3541 cgctgaccga attgggcgtg aatgtgggcg caaccaccgc gccggtgcgc gtggattatt
     3601 tgcagcctgc atttgacaag gcggcaacgg tggggacgct gttcgagccc gattacgaag
     3661 ccctgcaccg ctacaatcct cagcttgtca ttaccggcgg gccgggcgcg gaagcgtatg
     3721 aacagttagc gaaaaacgcg accaccatag atctgacggt ggacaacggc aatatccgca
     3781 ccagcggcga aaagcagatg gagaccttgg cgcggatttt cggcaaggaa gcgcgcgcgg
     3841 cggaattgaa ggcgcagatt gacgcgctgt tcgcccaaac gcgcgaagcc gccaaaggca
     3901 aaggacgcgg gctggtgctg tcggttacgg gcaacaaggt gtccgccttc ggcacgcagt
     3961 cgcggttggc aagttggata cacggcgaca tcggcctacc gcctgtagac gaatctttac
     4021 gcaacgaggg gcacgggcag cctgtttcct tcgaatacat caaagagaaa aaccccgatt
     4081 ggattttcat catcgaccgt accgccgcca tcgggcagga agggccggcg gctgtcgaag
     4141 tattggataa cgcgctggta cgcggcacga acgcttggaa gcgcaagcaa atcatcgtca
     4201 tgcctgccgc gaactacatt gtcgcgggcg gcgcgcggca gttgattcag gcggcggagc
     4261 agttgaaggc ggcgtttaaa aaggcagaac ccgttgcggc ggggaaaaag tagggagtcg
     4321 tctgaaaacg gagcttccga aggaagcggg gggtttctgc gaagctaaag tgcggtttca
     4381 acgaattgaa aagcagcctg tatgttgaaa ataccgctca agcaaaccta cggtttgccg
     4441 ccctctccct agccctctcc cacagggaga ggggattggg ttgcaggctg cctttaaggt
     4501 t
//
LOCUS       DAWGRZ010000160         4364 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000160 DAWGRZ010000000
VERSION     DAWGRZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.160"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(69..2045)
                     /locus_tag="WHT44_001749"
     CDS             complement(69..2045)
                     /locus_tag="WHT44_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ2533897.1"
                     /translation="MNEFSLAPIVIVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGITMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="WHT44_001750"
     CDS             2194..3033
                     /locus_tag="WHT44_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ2533898.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GETVIRDISALLGKPFVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARTRYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPTFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3232..4263
                     /gene="gap"
                     /locus_tag="WHT44_001751"
     CDS             3232..4263
                     /gene="gap"
                     /locus_tag="WHT44_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ2533899.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAAHDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         1111 a         1457 c         1013 g          783 t
ORIGIN      
        1 tgcgatttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggccggtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg acggacggtt
      301 tcgccgtttt cgccgaatcc tgcctcatca tcactaccga caaacagtcc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccgata cgacgcgccg
      421 ttgcctaaca ttgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg taaccaccac cattttcgcc cgtcccagac cggcggcttc caatacttcc
      661 ctgcgtttcg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacccgc
      721 gcaatgtcca agtcgagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagaaccgcc
      961 gccgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccctgcgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatca atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aaaatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatgc cggcaacgaa tgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacggcacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaat gccgacctgc aaaccgccca gaccgaacac cagccgcctc
     1801 atcgccctca acttgggcag cgagaactcc aaaccgatgc tgaacatcag gaacacaatc
     1861 ccgatttcgc ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaatcacaa taggggcaag cgaaaattcg
     2041 ttcatagacc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgcattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggaga aaccgtcatt
     2341 cgggacattt ccgccctgct cggcaaaccc tttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc ggatagacga aaccgcctgt atcggctgca ccgcctgcat ccgcgcctgc
     2461 cctgccgatg ccattatggg cgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgccgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgcac gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acgcccgaca caccgaaaaa accgacgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat atctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacagaa taccgcctcc tgacccttcc ctgatatgcc gtctgaagcc
     3061 gcttcagacg gcattttatc aagctctccg tccgccgcac ccgtgccgtc cgcatcttac
     3121 cgcccaccct tccagtcgcg ccgttttcaa taaaatatta attacacgcc actacaaatt
     3181 tgctataatc cgccccgaaa atctacccaa ccctcaacaa aggaacgaac catgggcatc
     3241 aaagtcgcca tcaacggcta cggacgcatc ggccgccagg ttttgcgcgc catctacgat
     3301 tatcagattc aagaccaact ccaaatcgtc gccgtcaacg ccagcggcag ccttgaaacc
     3361 aacgcccatc tgaccaaatt cgacaccgta cacggacgct ttgaagccga cgtatcccac
     3421 gacggcggca acctcatcgt caacggcgac aaaatcccct tcttctcgac ccgcaaccct
     3481 gccgaactgc cgtggaaaga actcggtgtc gatttggtca tggaatgcac cggcgcgttc
     3541 accagcaaag aaaaagccaa agttcatctg gaaagcggcg caaaaaaagt cctcatttcc
     3601 gcaccgggcg gcgacgatgt cgatgcaacc gtcgtgtacg gcgtgaacga cagcgtcctg
     3661 accgccgaca tgaccgtcgt ttccaacgct tcctgcacca ccaactgcct ctcgccggtt
     3721 gccaaagtgt tgagcgaaag cgtcggcatc gtcaaaggcg cgatgaccac catccacgcg
     3781 ctgaccaacg accaaaccgt taccgacgtg cgccacaaag acctgcgccg cgcccgcagc
     3841 ggcgtggaaa acatgattcc gaccaaaacc ggcgcggcaa aagccgtcgg tttggtactg
     3901 cccgaattga aaggcaggct cgacgggctt gccatccgcg tgccgaccgt caacgtatca
     3961 ttggtagatt tgagcttcca agccgcgcac gacacgacag tcgaagaaat caacgcactg
     4021 atgaaagccg cctcggaagc aggcgcactc aaaggcgttt tgggctacaa caccctgccc
     4081 ttggtttcca tggacttcaa ccacaccacc gaagccagcc acttcgacgc aacgctgacc
     4141 aaagtcgttg acggcaacat gattaaagtc ttcgcatggt atgacaacga atggggattc
     4201 agctgtcaaa tgctgaacac tgcacgccgc atgttcggac ttgaagtgcg cccgctcaaa
     4261 taagcaataa accgtcaaac aaaatgccgt ctgaaacccg atgttttcaa gttccagacg
     4321 gcatttttca ccgtgatttt acccggctgt cgtcatttcc aatt
//
LOCUS       DAWGRZ010000161         4357 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000161 DAWGRZ010000000
VERSION     DAWGRZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.161"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            123..1973
                     /locus_tag="WHT44_001752"
     CDS             123..1973
                     /locus_tag="WHT44_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ2533900.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPERKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LGARRQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVRRMAEDGAVKRALVLFQNSQ
                     SAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMANAAYYAALFGAPHIPLKQRMGIVPAR"
     gene            2262..2474
                     /locus_tag="WHT44_001753"
     CDS             2262..2474
                     /locus_tag="WHT44_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ2533901.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            2606..3052
                     /locus_tag="WHT44_001754"
     CDS             2606..3052
                     /locus_tag="WHT44_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ2533902.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTE
                     VEAAVAETGAAGMADMGKVMGLLKTRLAGKADMGEVNKILKAVLTA"
     gene            3073..3222
                     /locus_tag="WHT44_001755"
     CDS             3073..3222
                     /locus_tag="WHT44_001755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533903.1"
                     /translation="MPSEARIAGSDGIFNIPISNRQGQHGFDTPKRVLPINRFRLRPI
                     GQNAV"
     gene            complement(3251..3643)
                     /locus_tag="WHT44_001756"
     CDS             complement(3251..3643)
                     /locus_tag="WHT44_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ2533904.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(3715..4320)
                     /locus_tag="WHT44_001757"
     CDS             complement(3715..4320)
                     /locus_tag="WHT44_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ2533905.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
BASE COUNT         1059 a         1232 c         1205 g          861 t
ORIGIN      
        1 atgtcggaaa cattccaaag gcggtgcagt ttcggcatat aattcgggca aacgcctgtt
       61 cagacggcat tttgtctttt ccaaccctga ccgttcaggg ttccgattct taaggaaatc
      121 cgatgtacct accctctatg aagcattccc tgccgctgct ggcggccctg gtgcttgccg
      181 cgtgttcttc gacaaacaca ctgccagccg gcaagacccc ggcagacaat atagaaactg
      241 ccgacctttc ggcaagcgtt cccacccgcc ctgccgaacc cgaaagaaaa acgctggcag
      301 attacggcgg ctacccgtcc gcactggatg cagtgaaaca gaaaaacgat gccgccgtcg
      361 ccgcctattt ggaaaacgcc ggcgacagcg cgatggcgga aaatgtccgc aacgagtggc
      421 tgaagtcttt gggcgcacgc agacagtgga cgctgtttgc acaggaatac gccaaactcg
      481 aaccggcagg gcgcgcccaa gaagtcgaat gctacgccga ttcgagccgc aacgactata
      541 cgcgtgccgc tgaactggtc aaaaatacgg gcaaactgcc ttcgggctgc accaaactgt
      601 tggaacaggc agccgcatcc ggcttgttgg acggcaacga cgcctggagg cgcgtgcgcg
      661 gactgctggc cggccgccaa accacagacg cacgcaacct tgccgccgca ttgggcagcc
      721 cgtttgacgg cggtacacaa ggttcgcgcg aatatgccct gttgaacgtc atcggcaaag
      781 aagcacgcaa atcgccgaat gccgccgccc tgctgtccga aatggaaagc ggtttaagcc
      841 tcgaacaacg cagtttcgcg tggggcgtat tggggcatta tcagtcgcaa aacctcaatg
      901 tgcctgccgc cttggactat tacggcaagg ttgccgaccg ccgccaactg accgacgacc
      961 aaatcgagtg gtacgcccgc gccgccttgc gcgcccgacg ttgggacgag ctggcctccg
     1021 ttatctcgca tatgcccgaa aaactgcaaa aaagcccgac ctggctctac tggctggcac
     1081 gcagccgcgc cgcaacgggc aacacgcaag aggcggaaaa actttacaaa caggcggcag
     1141 cgacgggcag gaatttttat gcggtgctgg caggggaaga attgggtcgg aaaatcgata
     1201 cgcgcaacaa tgtgcccgat gccggcaaaa acagcgtccg ccgcatggcg gaagacggtg
     1261 cagtcaaacg cgcactggta ctgttccaaa acagccaatc tgccggtgat gcaaaaatgc
     1321 gccgtcaggc tcaggcggaa tggcgttttg ccacacgcgg ctttgacgaa gacaagctgc
     1381 tgaccgccgc gcaaaccgcg ttcgaccacg gtttttacga tatggcggtc aacagcgcgg
     1441 aacgcaccga ccgcaaactc aactacacct tgcgctatat ttcgccgttt aaagacacgg
     1501 taatccgcca cgcgcaaaat gttaatgtcg atccggcttg ggtttatggg ctgattcgtc
     1561 aggaaagccg cttcgttata ggcgcgcaat cccgcgtagg cgcgcagggg ctgatgcagg
     1621 ttatgcctgc caccgcgcgc gaaatcgccg gcaaaatcgg tatggatgcc gcacaacttt
     1681 acaccgccga cggcaatatc cgtatgggga cgtggtatat ggcggacacc aaacgccgcc
     1741 tgcaaaacaa cgaagtcctc gccaccgcag gctataacgc cggtcccggc agggcgcgcc
     1801 gatggcaggc ggacacgccc ctcgaaggcg cggtatatgc cgaaaccatc ccgttttccg
     1861 aaacgcgcga ctatgtcaaa aaagtgatgg ccaatgccgc ctactacgcc gccctcttcg
     1921 gcgcgccgca catcccgctc aaacagcgta tgggcattgt tcctgcacgc tgacgtaccg
     1981 atgccgtctg aaacccgccc ggtctttcag acggcatttt tatcccgaac ggcattgacg
     2041 gcgaaccata aatataagac aatccgaaaa ttgtttttcc tgctttttca agcagcttga
     2101 cacggcacaa gccgacccgt taggaggtga tgtttccgtc acggcgcgta tcccgccgcc
     2161 gcaaggcaca gcgatacggt aaactttcaa caccgtctgc cctacccttt ccaccgatat
     2221 gatgggcaga tgaaacaacc gaatttatta aaggaaataa aatgcctgca atccgcgtaa
     2281 aagagaatga accatttgaa gtcgctatgc gccgtttcaa acgcgccgta gaaaaaaccg
     2341 gcctgctgac cgagctgcgc gcccgcgaag cctacgaaaa accgactacc gaacgcaaac
     2401 gcaaaaaagc ggcagccgta aaacgcctgc aaaaacgcct gcgcagccaa caactgccgc
     2461 ccaaaatgta ctaaacgttc aagtacagat tacaggtcag ccctgtgata tgaggacaca
     2521 ccgcaagacc tgctctgcgg tgtgttttgc ttttcagacg gcatcgaaac ccgccgtttc
     2581 catccgacat cccagcgagg acatcatgag cctgaaaatc cgccttaccg aagacatgaa
     2641 aaccgcgatg cgcgccaaag accaagtttc cctcggcacc atccgcctca tcaacgccgc
     2701 cgtcaaacag tttgaagtgg acgaacgcac cgaagccgac gatgccaaaa tcaccgccat
     2761 cctgaccaaa atggtcaaac agcgaaaaga cagcgcgaaa atctacactg aagccggccg
     2821 tcaggatttg gcagacaaag aaaacgccga aatcgaggta ctgcaccgct accttcccca
     2881 aatgctttcc gccggcgaaa tccgtaccga ggtcgaagct gccgttgccg aaaccggcgc
     2941 ggcaggtatg gcggatatgg gtaaagtcat ggggctgctg aaaacccgcc tcgcaggtaa
     3001 agccgacatg ggcgaagtca acaaaatcct gaaagccgtg ctgaccgcct gattgcccga
     3061 atatcggaca aaatgccgtc tgaagcccgt atcgcaggtt cagacggcat tttcaatatc
     3121 ccaatatcga atcggcaggg gcaacacggt tttgatacgc cgaaacgggt tttgccgata
     3181 aacagattcc gtttgcgccc catcggacaa aatgccgtct gaaacacgat tccgttcaga
     3241 cggcatagat ttatttgacc aatttcaagc cttttttggc gggtcggggc gcggtttcgg
     3301 cagaggtgtt ttcaggcggc gtatcggggc ggtacgcttc caactcaaac cccatacctt
     3361 ctccggtctc ccgtgcgaaa aggctgagga cgtgtccgac aggtatccat atatcgtgcg
     3421 cctgtccgcc gaagcgggcg gaaaagctga tccaatcgtt gtcgatttga aggttttgcg
     3481 tggcggtcgc gccgatgttg agcataattt cgttgtcgcg gacgtactgc atggggacgc
     3541 gcgtgtgttc gttgacccag acaaggatgt gcggtgtgag gctgttgtcg ctgcaccatt
     3601 cgcagagggc gcggaggatg tagggtttgg tggaagtggg cataatgggt tccgtgttgt
     3661 acgccaaaat aggaaaatgc ctgcaaaacg gtgggttttg caagcatttc ggacttattt
     3721 gcgcatggct ttttcggcgg gtgtcagtgc ttcgataaag gcttcgcgct ggaagatgcg
     3781 ctcggcgtat ttgagcagcg gcgcggcact tttgcccagt ttgacatcgt agtggtcgag
     3841 ccgccacagc agcggagcaa gggcgacatc aatcatagaa aaatcttcgc cgaggatgta
     3901 tttgcttttg ctgaacgaag gggcaagcat ggtcagaccg ttgccgatgg cttcgcgcgc
     3961 ttttgcctgt tccttgttgg tggcggcggg gttttctaac acttggacgt ggttgaacaa
     4021 ttctttttcc atacggtaca gcaccagccg gccccgaccg cgcataacgg gatcgccggg
     4081 catcagctgc ggatggggga agcgttcgtc aatgtattcg ttgatgatat tggactcgtg
     4141 cagcaccaaa tcgcgctcga ccagcacggg aacttggtta tacggattca tgacggcgag
     4201 gtcttcgggt ttgttgtaaa tatcgacgtc tttgatttca aaatccatac ctttttcgta
     4261 caaaacgaag cggcagcggt ggctgaaggg gcaggtaatg ccggaataga gggtcatcat
     4321 aataattgtc gctcctgtgt gatgcctgca aaacggc
//
LOCUS       DAWGRZ010000162         4302 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000162 DAWGRZ010000000
VERSION     DAWGRZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.162"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            237..1157
                     /gene="ribF"
                     /locus_tag="WHT44_001758"
     CDS             237..1157
                     /gene="ribF"
                     /locus_tag="WHT44_001758"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ2533906.1"
                     /translation="MRIRLGRHNAPDFPQGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQGLNVRF
                     LHKLRDEEKFDGMEELKRQIEADMEAARRW"
     gene            1299..4088
                     /gene="ileS"
                     /locus_tag="WHT44_001759"
     CDS             1299..4088
                     /gene="ileS"
                     /locus_tag="WHT44_001759"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ2533907.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDHPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKISPAIDVAYLFKDTAA
                     LAAAFGLAGFEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGRYIGETPRVAGMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVALKIEEKGIE
                     AWFSLDKSELLSAEDCENYDKLSDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYAVVLARQLQECLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSHARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKTEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         1054 a         1181 c         1140 g          927 t
ORIGIN      
        1 tctcggttta cctggttaaa aaagaacgat tttcactgat gttgcatcag gtttggggcg
       61 atgtttcaac acatagcacc gcgcctgctg cgcgtttttg tgcgtttggc gcgttcggcg
      121 gcgggaaatt tgcctacttt tcccgcgtcg ggcgggcgta acgggcggca cactgtctat
      181 aaaccgcaat accgtttaca atgaccgcct gtttcaccac atacccgaat gcaacaatga
      241 gaatcaggct ggggcggcac aacgcgcccg actttccaca gggtgccgcc gtaaccatag
      301 gcaatttcga cggcgtacac ctcggacaca aacacatcct ccaaaaactc cgcctcgaag
      361 ccgacgcgcg cggactgccc gtcgtgaccg tcgttttcga accccaaccc aaagaatttt
      421 tcgcactccg caccggcagg atgccaccgt gtcggatcag ccccctgcgc accaagctcg
      481 aattattgga aggcacaggc tgtgtcgatg ccgtctgggt tttgcgtttc gatcaaaatt
      541 tttccgaaat atccgcgcaa gggtttatcg accgcctgct gcgtcaaacc ttgaatacgc
      601 gttatttgct cgtcggcgat gatttccgtt tcggtgcggg gcgggaaggc tgttttgaac
      661 ttttggcaca acagcccgat atgcagaccg agcgtacgcc ttccgtcatc gtcgaagaca
      721 tccgcaccag cagtaccgcc gtgcgacaag ccctttcaga cggcaacctt gcctatgcga
      781 aaaaactttt gggacacgac tacgtcttga gcggcagggt ggtgcacggc agaaaactcg
      841 gacgcacctt aaacgccccg actgccaaca tccgcctgcc ccgccaccgt tatgcactcg
      901 gcggcgtgtt tgtcgtcgaa gcagacggcg cattcggcac gcggcgcggc gtggcgagtt
      961 tcggcttcaa tcccaccgtt gatagcggct gttctcaaaa gcttgaagtc cacctgttcg
     1021 actttcaagg cgacctgtac ggacaagggc tgaacgtccg cttcctgcac aaactgcgcg
     1081 atgaggaaaa gtttgacggt atggaagaac tgaaaaggca gattgaagcc gatatggaag
     1141 ccgcaaggcg ttggtagaaa aaccttatac aaaccatccg attgggctac aatcaatctt
     1201 ttaactgttc agacggcaca gggttttccc gttgtgaaat gctgtttggg gcgcaatgcc
     1261 gtctgagacc gaaatattgt aacaatagag attaaaaaat gaccgattac agtaaaaccg
     1321 taaacctgct cgagagcccg tttccgatgc gcggcaatct tgccaagcgc gagcctgcat
     1381 ggctgaaaag ctggtacgag caaaaacgct accaaaaact gcgcgaaatc gccaaaggcc
     1441 gtccgaaatt tattctgcac gacggcccgc cgtatgccaa cggcgacatc cacatcggtc
     1501 atgccgtcaa taaaattctc aaagacatca ttatccgcag caaaacccaa gccggttttg
     1561 acgcgcctta tgtgccgggt tgggactgcc acggcctgcc catcgaagtg atggtagaaa
     1621 aactgcacgg caaagatatg cccaaagcac gtttccgcga attgtgccgc gaatacgccg
     1681 ccgaacagat tgcccgtcag aaaaaagact ttatccgctt gggcgtgttg ggcgactggg
     1741 accatcctta cctgactatg gatttcaaaa ccgaagccga taccgtgcgt atgctcggcg
     1801 aaatctacaa atccggctat ctctaccggg gtgcgaaacc ggttcaattc tgcttggact
     1861 gcggttcttc gctggccgaa gcggaagtgg aatacaaaga caaaatctcg cccgcgattg
     1921 acgttgccta tctgtttaaa gacactgccg cgcttgccgc cgcattcggt ttggctggtt
     1981 tcgaaggcaa agcgtttgcc gtcatttgga cgactacgcc ttggacgcta ccggcgagcc
     2041 aagccgtgtc tgcgggtgca gacgtggtgt atcaactgat tgatacgccg aaaggcaaat
     2101 tggtattggc gaaagatttg gcagaagacg cgctcaaacg ttacggtttt tcagacggca
     2161 ttgctattct cgccgaaacc accggcgaca agctggaaaa tctgcacatg aaccatccgt
     2221 tcctcgaacg cgatattccc atgctcaacg gcgaacacgt taccaccgat gccggtaccg
     2281 gcttggtaca caccgccccc gcgcacggtt tggaagacta cgccgtctgc aataaatacg
     2341 gcatcgagct ttacaacccc gtcaacgccg aaggccgata catcggcgaa acgccgcgtg
     2401 tcgccggtat gcgcgtttgg gaggcgaacc ccgtcatcct gcaatggttg gaagaaaccg
     2461 gcaacctttt ggcaagcagt aaaatcgaac acagctacgc ccactgctgg cggcacaaaa
     2521 cgccgctgat ttaccgcgcg acaggtcaat ggtttgtcgg tatggacaaa gccggtgccg
     2581 acggcaaaac cctgcgcgac aaagccatca aggccgtgga cgacaccgaa ttcttcccgt
     2641 cttggggtcg cgcgcgtttg gaagccatga ttgaaggtcg tcctgactgg gtggtttcac
     2701 gccaacgcta ttggggcacg ccgatgactt tctttgttca caaagaaacg ggcgagctgc
     2761 atccgaactc tgccgaactt ttggaaaaag ttgccctgaa aatcgaagaa aaaggcatcg
     2821 aagcgtggtt ctccctcgat aagagcgaac tcttgagcgc ggaagattgc gaaaattacg
     2881 ataaactttc tgacacaatg gacgtatggt tcgactccgg ctcgacccat tattccgttg
     2941 tgaaacaacg cgaagaattg gaatggccgg ctgatttgta tctcgaaggc agcgaccaac
     3001 accgcggctg gtttcaatcg tccatgctga ccggctgcgc ctcatcaatg ggtcgcgcgc
     3061 cgtataaaca gctgctgacc catggtttcg ttgtcgacgg cgaaggcaaa aaaatgtcga
     3121 aatccatcgg caacgtcgtt gcaccgcaag aggtttataa cgaattcggc gcagacatcc
     3181 tgcgcctgtg ggcggcatct accgattaca gcggcgaatt ggcgatttcc aaagaaatcc
     3241 tcaaacgcgt aaccgaaagc taccgccgta tccgcaatac cttgagcttt ttgtttgcca
     3301 acttgagcga ctttaatccg attgaagatg ccgtgcaaca ggcggatatg gtggaaatcg
     3361 accgctacgc cgtggtattg gcacgtcagc tgcaagagtg tctggcaggc gattactatc
     3421 cgcgttatgc cttccacttt gccgtaaaag acattgtttc tttctgctcg gaagacttgg
     3481 gtgcgttcta cctcgacatc ctgaaagacc gcctctacac caccaaagca gacagccatg
     3541 cacgccgcag cgcacaaact gccctgtatc acatcacacg cagtttggtt ctcttgattg
     3601 caccgatttt gtgcttcacc ggcgaagaag cgtgggacat catcggcggc ggcgaagaag
     3661 acagcgtcct cttccatact tggcacgagt tcccgaccat caacgaaaaa accgaagccg
     3721 aactggtgaa aaaatggacg gcaatccgcg aagcccgcga agcggtaacc gccgccatcg
     3781 agcctttgcg cgccgacaaa accgtcggtt cgtccttgca agccgaagcc gaaattaccg
     3841 cgccggaaga aatggccggc tatctgaatg ctttgggcga agaattgcgc tttgctttgc
     3901 tggtgtctaa agcagaagtg aaagtaggca gcgaacttgc cgttgccgct aaagccagtg
     3961 atggtgaaaa atgcgaacgc tgctggcact acacccgcga tgtgggcgcg gttgcaggct
     4021 atgaaaccgt ctgcaaacgc tgtgcagaga atgtcggcgg agaaggcgaa acgcgccatt
     4081 acgcctgata aagtttgagc aaatgccgtc tgaaaccgcc gaacagcatt tcagacggca
     4141 ttttttgtgc cgcgatttgt ctttataacg gcggaggggt ttcaagattg cggtgttgtc
     4201 ggaatgcaac tgaaccgtca ttcccacgaa agtgggaatc tagaatctcg aggtttcagt
     4261 catttccgat agattcccgc ctgtgcggga atgacggatt tc
//
LOCUS       DAWGRZ010000163         4301 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000163 DAWGRZ010000000
VERSION     DAWGRZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.163"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            579..2306
                     /gene="ilvB"
                     /locus_tag="WHT44_001760"
     CDS             579..2306
                     /gene="ilvB"
                     /locus_tag="WHT44_001760"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ2533908.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKVDIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADGKA"
     gene            2317..2808
                     /gene="ilvN"
                     /locus_tag="WHT44_001761"
     CDS             2317..2808
                     /gene="ilvN"
                     /locus_tag="WHT44_001761"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ2533909.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            2875..3168
                     /locus_tag="WHT44_001762"
     CDS             2875..3168
                     /locus_tag="WHT44_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ2533910.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            3248..4261
                     /gene="ilvC"
                     /locus_tag="WHT44_001763"
     CDS             3248..4261
                     /gene="ilvC"
                     /locus_tag="WHT44_001763"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ2533911.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
BASE COUNT         1119 a         1031 c         1081 g         1070 t
ORIGIN      
        1 gcttcgtcgc cttgtcctga tttttgttaa tccactatac aatcaatata ttaagtcgtt
       61 gttagccctc ttggttctgt caatgccgtc caatacagtc cggtgcaatg agccattttc
      121 aggaagtatg gttgggaata tttttgatat atcaaaaata aaatcaagtt caatcaatat
      181 cccgagtctt tattgggatg aaaccctgta aattttacaa ttcttgatat cgaagaaggg
      241 tttttcacat ctgcccctcc tacacaaact gtcaacaatt tccatctttc aaaaaaggta
      301 ctcctgattt tattctttta aaatcaatca ataatttctc tgctttcaaa aaatattaaa
      361 agatgaaaga tttttcttga ttgaactgtc ggtttgcgtt atcgtttcta ctctttttca
      421 caaactctgt gttcctttcc aattgattgg ataatccgtc tgttgccgtg ttcccccaat
      481 gcgcgtaccc taaatcgctg cttggtggaa ttgcattaca ggtgctgtgg caaagcggtt
      541 tgtccctatt ggtttgaaac cgtataaaag aggtcgttat gcagttatca ggcgcgcaaa
      601 tcatagtgca gagtctcaaa gccgaaggtg tcgagtacgt tttcggttat cccggcggtg
      661 cggttatcga aatctacgat gcccttttcc aactcaataa attcaaacac attcttaccc
      721 gccatgagca ggcggcagta cacgcggcag atgcgtatgc gcgcgtcagc ggtaaggtgg
      781 gcgtggcatt ggttacatcc ggcccgggcg ttaccaatgc actgaccggt attgctactg
      841 cctatacgga ttcgattccg atggtggtca tcagcgggca ggtaggcaat tccctgattg
      901 gtacggatgc gttccaagaa gttgatacgg tgggtattac ccgtccgtgc gtcaaacaca
      961 atttcctggt tacggacatc aatgagttgg cggaaaccat taaaaaggcg ttccaaattg
     1021 ccgcaagcgg ccgaccgggg cccgtggtgg ttgatgtccc gaaagatgtt acgcaggcga
     1081 tggcgaaatt cagctatcct caggaagaca tttttatccg ttcgtatcaa ccggttgttc
     1141 aaggacatat cggtcagatt aaaaaggccg tgcagatgtt ggcatctgcc aaacgtccgg
     1201 tcgtctattt tggcggcggc gtggtgttgg gtaatgcttc tgaagagctg acccgatttg
     1261 tccgaatgac gggtgctccg tgcacgggta cgctgatggg cttgggcgct tatccttccg
     1321 gcgaccgcca attcctcggt atgctcggta tgcacggtac ttacgaggca aaccttgcca
     1381 tgcagaatgc ggatgtcgtt cttgccgtag gtgcgcgttt tgacgaccgt gtggtttccg
     1441 taccgtccaa atttttcgag aaggcgaaga aggttatcca tatcgatgtc gatccttcca
     1501 gcatcgccaa acgcgtgaag gtggacattc cgattgtcgg cgacgtgaaa aacgttttgt
     1561 ccgagatggt tgcgctgtgg caaaaacaag agtccgtgcc gtctgaagat gctttgggca
     1621 aatggtggaa aaccatagag gaatggcgtt cccgagattg cttgtggttt gacaacggca
     1681 gcgaaattat caagccacaa tatgtgattc agaagcttgc cgagattacc ggcaattcgg
     1741 caatcatcac atcggatgta gggcagcatc aaatgtttgc ggctcaatat tatcccttcg
     1801 aacgtccgcg ccaatggctc aattccggcg gtttgggtac gatgggcgtg ggtctgcctt
     1861 atgcgattgg tgcaaaactt gccgccccgg atcaagacgt attctgtatc accggcgacg
     1921 gctcgatcca gatgaacatc caagagctgt ccacctgttt ccaatatcgg attccggtta
     1981 acgtcattac gctgaacaac ggttatctcg gtatggtacg ccagtggcag gaaatatatt
     2041 acggcggacg agagtcggaa acctatttcg attctttgcc cgatttcgtc aaacttgccg
     2101 aggcatacgg ccatatcggt atccgcgtgg acaagaagtc tgatgtggaa ggtgcgttgt
     2161 tggaagcatt gaaccaaaaa gacaggctgg tgtttatcga cttcctgacc gaccagaaac
     2221 agaatgtgat gcccatggtc ggcaacggca aaggtttgga cgaaatggta cttccgccgc
     2281 atatgcgtgc ggacggaaag gcgtaaggag agacaaatgc gacatatctt atctgttctg
     2341 attgaaaacg aatcaggtgc gatgagccgc gtggtcggtt tgttctctgc acgcgattac
     2401 aatatcgatt ctttggcggt tgcgccgacc gaagacaaaa ccctgtcacg gatgaccatc
     2461 gttacccacg gcgacgagca ggtcatcgaa caaattacca agcaactcaa taaattgatt
     2521 gaagtgatta aagtggtcga tttgaatgaa agccgttttg tcgaacgcga actgatgttg
     2581 gtaaaagtcc gtgccgccgg caaagaccgc gacgaatttt tacgcttgac cgaaatctac
     2641 cggggcagca tcatcgacgt aaccgaccgc agctatacga ttgaaattac cggctcgaca
     2701 gacaagctgg attccttttt ggaaacggtc ggacgcgccc aaattttgga aaccgtacgc
     2761 acaggcgcgg ccggcatcgg tcgcggtgag cgtattttga aaatttaacg ccgtaacctt
     2821 tcagacggca cggtatttga atgtcgtctg aaaaacgaac ggcaggagag atttatgtcg
     2881 aacattaaaa ttgtcgcact ggttaccgtc aaaccggaat acacagaaac actggcagca
     2941 cagtttaaag aactggtcaa agccagccgt gcagaagagg gcaacatcag ctacgatctc
     3001 catcaggaaa tcggcaaacc gaaccgtttt gttttcgtgg aaaattggaa atctcaagca
     3061 gctattgacg aacataacgc cagcgcgcat ttccaagcct tcgtccagtc cgtcgacggc
     3121 aaaaccgaag cgttggaaat cgtattgatg aatgaagttg ccgattaagc ggcacactct
     3181 gtttaacccg tccgaagccg ctcaacattt taaggcttcg acaaccattt accttaaagg
     3241 aaatcaaatg caagtctatt acgataaaga tgccgatctg tccctaatca aaggcaaaac
     3301 cgttgccatc atcggttacg gttcgcaagg tcatgcccat gccgccaacc tgaaagattc
     3361 gggtgtaaac gtggtgattg gtctgcgcca aggttcttct tggaaaaaag ccgaagcagc
     3421 cggtcatgtc gtcaaaaccg ttgctgaagc gaccaaagaa gccgatgtcg ttatgctgct
     3481 gctgcctgac gaaaccatgc ctgccgtcta tcacgccgaa gttacagcca atttgaaaga
     3541 aggcgcaacg ctggcatttg cacacggctt caacgtgcac tacaaccaaa tcgttccgcg
     3601 tgccgacttg gacgtgatta tggttgcccc caaaggtccg ggccataccg tacgcagtga
     3661 atacaaacgc ggcggcggcg tgccttctct gattgccgtt taccaagaca attccggcaa
     3721 agccaaagac atcgccctgt cttatgcggc tgccaacggc ggcaccaaag gcggtgtgat
     3781 tgaaaccact ttccgcgaag aaaccgaaac cgatctgttc ggcgaacaag ccgtattgtg
     3841 cggcggcgtg gtcgagttga tcaaggcggg ttttgaaacc ctgaccgaag ccggttacgc
     3901 gcctgaaatg gcttacttcg aatgtctgca cgaaatgaaa ctgatcgttg acctgatttt
     3961 cgaaggcggt attgccaata tgaactactc catttccaac aatgcggagt acggcgaata
     4021 cgttaccggc cctgaagtgg tcaatgcttc cagcaaagaa gccatgcgca atgccctgaa
     4081 acgcattcaa accggcgaat acgcaaaaat gtttatccaa gagggtaatg tcaactatgc
     4141 gtctatgact gcccgccgcc gtctgaatgc cgaccaccaa gttgaaaaag tcggcgcaca
     4201 actgcgtgcc atgatgcctt ggattactgc caacaaattg gttgaccaag acaaaaactg
     4261 attgttttca aacgggacta cctacacatc gtgtaggcag t
//
LOCUS       DAWGRZ010000164         4282 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000164 DAWGRZ010000000
VERSION     DAWGRZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.164"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(257..1699)
                     /locus_tag="WHT44_001764"
     CDS             complement(257..1699)
                     /locus_tag="WHT44_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ2533912.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSLFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQ
                     QQAKAAFLPHVSANASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQ
                     AAEQRFDAAREELLLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAAT
                     ALDIHEAKAGYDNALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPK
                     LERYSLDEWQRIALSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTS
                     SAQNNDYHYRGKGMSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLA
                     VRQAYTESGAARYQIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQ
                     KLAQARYKFMLAYLRLVKESGLGLETVFAE"
     gene            complement(1768..3195)
                     /locus_tag="WHT44_001765"
     CDS             complement(1768..3195)
                     /locus_tag="WHT44_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ2533913.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLKPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQNRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVIAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            3431..3784
                     /locus_tag="WHT44_001766"
     CDS             3431..3784
                     /locus_tag="WHT44_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533914.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            4073..>4282
                     /locus_tag="WHT44_001767"
     CDS             4073..>4282
                     /locus_tag="WHT44_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533915.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILR"
BASE COUNT         1014 a         1121 c         1055 g         1092 t
ORIGIN      
        1 gtttgggcga atattaagcg gtatctgcga accgtattgt ctgattacgc ccgatttaac
       61 gatgcactac tgtcctattt tgattttaat tgaatataca tttgaattaa ttcgcactta
      121 atttaaatgt gtttttaact gtgctttatt taaaggcaat gagaatgtga aaatatcgga
      181 tcaatcccaa agcagcctgc actttcgaaa cggggtgcag gctgctttgg gaatttcata
      241 accgtttcag cctgctttat tccgcaaata ccgtttccaa ccctaacccg ctctctttca
      301 ccaagcgcaa ataagccagc atgaatttat accgtgcttg agccagtttc tgttctgctt
      361 gggcgacttc ctgccgcgcc cgtattactt ccagccggtt gcggatgccg tattgttggc
      421 cggtttcggt cgatttcagt ttcaaacggc tgctttccaa aacccgttct tgcgccatga
      481 tttggtaacg cgccgcaccg ctttcggtat aagcctggcg tacggcgagt ttgatgtgcc
      541 gctcggttgc ggtcagctgt gcttcggcgg ccccgtattg cgcttcggct tcatggattt
      601 tgcccgacaa ttctccgccg gtataaagcg gcaaattcaa ctgtacgccg acgctcatcc
      661 ctttgccccg atagtggtag tcattattct gcgcagatga agtgtagagg ttattctgat
      721 agccgacatg ggcagaaacg gtgggatagc ggctgttctg tgctgcccga agcgcctgtc
      781 cgctgctttg cagggcaagc tgctgcatcc ggtattcatg attgttggat aaggcaatgc
      841 gctgccattc atccagactg taacgttcca gcttgggcag atagcgtgcc aacaggttgg
      901 cggtatctat ggcctcgatt tgtttgctat ccaggtcggt gtagtcgttc aactggtttt
      961 cataggtttg tttctcagcc aatacggcga tttcttgggc cagggcattg tcgtaaccgg
     1021 ctttggcttc gtgaatatcc agcgcggtgg cagcaccttt attgaataaa gcctgcgcct
     1081 gccttacctg ctgggcataa gcctcttttt ccgccgcatg ggcggcaacg gtgtctcggc
     1141 tgagtaaaac gttgaaataa ctttcggcaa ctttcaacag caattcttcg cgtgccgcat
     1201 cgaaacgctg ttctgcagcc tgcgtatcga acctgctttg gcggtattgt gcaaatttgg
     1261 cagcgtcaaa taaggtttgt cccacctgca cgctccatcc ctgtgtttcg cgggtggaag
     1321 aaatcgatgg cggctggcgc tggtagctgg cattggcgga tacatgggga aggaatgcgg
     1381 ccttggcttg ttgttgccgt gcgcgcactg catcacgctg gtaatgggac gcttgaaaat
     1441 cagccgaatg ttgctgcgcc gcccgccatg cttcaggcag cgtaaaagcc gaaacggatg
     1501 gggaaaggga tagtggcaag gtaaaaagtg aaacggatgg ggaaagggat agtggcaagg
     1561 taaaaagtga aacgggtagg atatatttgg aaaaatagga tttcatagcc gaaaatagtt
     1621 catgttgcaa atagggcgtc agtgtcaggc aaacggaaat accgtaatct tgcattatca
     1681 ttagattgag caatgtcatc cgggcaatgg tttcaggcag tctgcatgtc cgaaccggcg
     1741 gataacaaat gcccagtacg gatccgccta tcgctcccta aagctttcgt ccaatttggt
     1801 ttgcagcggg cttaacagat aatccagcac ccgccgttta cccgttttaa tctccgccgt
     1861 gacattcatg cccgccgtca gattcactgc tttgccgtca atattcaagg tatgtttgtc
     1921 cagcgacacc accgccgtat aaaccaagcc caactgttcg tggcttaccg catcatggct
     1981 gacacttttc accttgcccg tcagataacc gtagcgcgta tagggaaagc tctcaatctt
     2041 caccaccgca tcctgtccct gttccacaaa accgatgtct ttgttcaata ccaaaacttc
     2101 cacgtccatt ttgtcgtcat cgggcgcaat caccatcatt ttttgggcag cctgcaccac
     2161 accgcccacc gtataggtag ccaattcctg caccgtgccg tccgcaggcg actgtattgt
     2221 catcagctgc tgccgctgct ttgccttatc cgtttggccg cggtattggt caatctgttc
     2281 gtttgcctgg cgcagcgcat ccagcgtatc gcgtttcagg ttctgcgtat tcagcacccg
     2341 attctgctcc gcctgtgcaa tggccgcctg aatctgcctc atctgaccgc gcgtactttc
     2401 caaatcgttc caattgctga ccgatttgct ctgctgctcc aaaaacgcat gttccgaaat
     2461 aaaattgtcg gcccgcaaac ggcggtagtc tgctgttttc tgctgctcga tcgcccccac
     2521 cgaaaccagc ttctgctcct gcgccttggc cgactgcaat tccgcctgat ggccgcgcaa
     2581 agccgactgc aattgcgcat cctgcgccgc ccatgcctga tactggtgct gcgccaacac
     2641 ctgcgccgat tgcacatcgg catcggagag acctaaagac cgtgcttgcg ccatatcgat
     2701 atgcggcacg gtacggcttt ccaatgccgc caataccgct tcataacgca gtttggacaa
     2761 ttgggcagcc tgcaaagcct gctccgactg caccacatcg ctgtctgttc ccacagcctc
     2821 cagttccgcc agcgtttctc cctgtttcac atgctgcccg tcgcgcacat gtaccgcctt
     2881 aaccaccgcc gtttccagcg gctggatggt tttgctgcgc ccgcccgaca ccgttttgcc
     2941 cgaagccgcc gccacaatat cgattttgcc gaaccaggac cacaacaaag ccaaaagcgc
     3001 aaacgccata ataaaacgcg ccgcccattt cggagcggca gagaccggcg tatcggtcag
     3061 ttccaaatgc gcgggcaaaa acgcctgttc ttccgccgtg cgtttgggcg gtttcaactg
     3121 gtcgcgcacc gcccaaacat tgcgccatac agtaatgtat cgagaaagaa aggatttcag
     3181 ggcggagaaa aacataacgg gtataacctt ggcaatatag aaacaggaaa caatataaat
     3241 atgtaaagga attttaacgg aaagcgcggc agctgttaag ggaaaggcgg gaatattgac
     3301 aaaaaatacc caagtcgtta caaatattca ttattttact gcgtaacgca acgctgaagc
     3361 gcaggctgct tttgagatgc ggcaaggttc ggcaaaaagc agcctgcaca tttaaccaca
     3421 ggaacaaccc atgtttacca caaacgattt acgccatttc ctagaaggtt tggccatcct
     3481 attctcaatc ggctattggg gcaccatgct gctgttgctt tggtttctcg tccgctttgc
     3541 ctataaaaag cccaaacgga accccggcaa aatcctttca ggcagcctga ccgccgccgc
     3601 tatcctgatg ctgtttgttt ggattattcc caaacaattc ggcccgatca aagaagaaat
     3661 acaggcacaa gaagagtggg acagaaaata caaagaagcc gaagccgtgt ttaacgaaca
     3721 atgcaaaacg gcgggggaaa gatttaccag acggcggaca atgtggaagg gattatgctg
     3781 ttgaaggtag tacctgagcg taccgtttcg gcagatgcaa aaaccagaga cccgatgtgg
     3841 gacaatgcgg ctttacagac cagcgaaggc gtaaatttta ttgctcgttt cctaggattt
     3901 tttagcgatg gggaataccg ctatgtggat gtcctgcaac ccaaccattc cgatattatt
     3961 cggtattcag gtaaagattt ttccgctaaa tcaaatattt aatcatatac accccgcccg
     4021 ttatgcggta acgttcgaaa acaatgtcga ttccaagctg cgcaggcact gggtggcagg
     4081 tgcgaccata cggattatcg accgccaaac tgacgaagtg attgccaaga aaaccatcta
     4141 tgtctttgaa aaaggcttgg acggcacggg tggggcgaga atgccgtgga agtttgctat
     4201 cttgtgcaat aaagaaagac ttacttcttc agagccgtta tcggattttg ttcttagcgt
     4261 tttaaaacct tatatattgc gt
//
LOCUS       DAWGRZ010000165         4214 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000165 DAWGRZ010000000
VERSION     DAWGRZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.165"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(77..313)
                     /locus_tag="WHT44_001768"
                     /pseudo
     CDS             complement(77..313)
                     /locus_tag="WHT44_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            509..967
                     /locus_tag="WHT44_001769"
                     /pseudo
     CDS             509..967
                     /locus_tag="WHT44_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            867..1235
                     /locus_tag="WHT44_001770"
     CDS             867..1235
                     /locus_tag="WHT44_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ2533916.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            1304..2842
                     /gene="lnt"
                     /locus_tag="WHT44_001771"
     CDS             1304..2842
                     /gene="lnt"
                     /locus_tag="WHT44_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ2533917.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASNNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMDMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILALAALILFIFR
                     NKEH"
     gene            3107..3970
                     /gene="rpoH"
                     /locus_tag="WHT44_001772"
     CDS             3107..3970
                     /gene="rpoH"
                     /locus_tag="WHT44_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ2533918.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNRIPMLTQEEETRLAERQHK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
BASE COUNT         1038 a         1300 c          966 g          910 t
ORIGIN      
        1 accttgccgt cgggcaggat gtaccacgcc aaatccccgc ctgaagcacg gtgtcggact
       61 gcatttctat tcggctatac gccttttcac gcaggctgta attgaatttc cacaaaccgc
      121 cgaacactgc cgacggacgc gcgaccagca tcaggaacgc cgcaatcgcc aaggcttccg
      181 ccgccctgtc caacacgttg gcaggagaaa ccagcagcaa aggcattccc aaacgtgcgg
      241 acaaagtggt cgaaaccacg ctcagaaaca acagtgcgcc acccggcagg aacagactgc
      301 ccatccaatc cacgctaacg attctccctt aaaattcatg aaattaaaat cgaaagactg
      361 taacacggta ttttcttttc acgaaacttt acttcacgtc cgccacagcg ttcagacggc
      421 attttcctgt ttcatgccgc ctgaaaccgc ccaaatccgc gttctcctat ataatgtcgg
      481 actttgcgcc gcatttcagg attctttaat ggcatttgcc tcactattta ccctgccgga
      541 cgacatcacc gccgttttgg acgatgttgc cctgatgacc aaagccgccg caaagaaaac
      601 cgccggcgtg gtcggcgacg atttggcacc caatgccaac cgggttacgg gcgtatcggc
      661 agaacgcgag ttgccgattg tttggtcgat ggcgaaaggt tctttttcca acaagccggt
      721 tttggtgcct gcggccctgc tgctttccgc cttcctgccg caactcatta cgccgctttt
      781 gacggcaggc ggcatttatc tgtgctttga aggcgtagaa aagctgctgc acaagtttct
      841 gcaccgacat gacgcgcatg ctcgtaatgg cggcaatcgg catcggcatg actgttttgg
      901 tgtacggaat cgttgccgtc atcgtcaaac tcgacgactt aggcatgctg ctgatgcgcc
      961 gcccacaaac cttttcgcgc agtttggggc aaatgctgac tgcctttatg ccttgcttta
     1021 tgcgcggttt gagcgtggtc ggcacgcttg ccatgtttct tatcggcggc gtactggtcg
     1081 cccacaacct cggccttctg cacgatttcc tgcacgcgca acactgggac gctggctggg
     1141 cggagtactt cgccaacctc gttgtcgggc tgctttccgg ttcgattgcc tgcgcccccg
     1201 ccttgccgct gatgaatcgt ttcggcaggc attgattcct ttttcaaata ccgatgccgt
     1261 ttgaaagatg ttcagacggt atcttccgaa cagacagatg aatatggttt ccaaactgga
     1321 caaatactgg cagcaccccg ccctctactg gcctttgctc atcctttttg ccgccgccac
     1381 cccctttacc ttcgcaccct actaccactt ttggctgatg cccttgattt tcggtgcctt
     1441 cgtccgcctc atcgaactgc gtccgcgttt tgctgtctct tccgcctacc tgttcggcct
     1501 gaccgcatac acgacacagt tctactggat acacaccgcc ctgcacgacg tttccggcct
     1561 gcccgacctc tatgccgtac cgctgacctt cctactcccc gcctaccttg ccctttatcc
     1621 ggcactgtgt ttctggctgt ggaaaaaatt taccctgcct cggggcataa aaatcggttt
     1681 ggtactgccc atcctgtgga cgctgaccga gtttgcccgc gaacgtttcc tgaccggatt
     1741 cggctggggc gcaatcggct actcccaaat caccccggac agcccgctcg ccggctttgc
     1801 cccattgggc ggcatccaca tggttacact ggcaaccgcc tttctcggtg tctggctggt
     1861 tttggcgagt aataacaccg cacgttcggg caaacgcctg cttccgatta tcctgattgc
     1921 cgccctgctt gccgcaggct acaccgcccg acaaaccgac ttcacccgcc ccgacggcag
     1981 ccgcagcacc gtcgccctgc ttcaaggcaa catcgaccaa accctcaaat ggcgtgaaga
     2041 ccaagttatc ccgaccatac agaaatatta cgaacaagtc ggcaaaacca ccgccgacat
     2101 cgtcatcctg cccgaaaccg ccatccccgt tatgcgccaa aacctgccgg aaaacatact
     2161 ggcgaaattt gccgaacagg cgcaaaacaa cggcagcgcg ctcgccgtcg gcatcagcca
     2221 atacacttcg gacggcaacg gttacgaaaa cgccgtcatc aacctgaccg gttatcagga
     2281 aaacaatcag gacggtatcc cctactacgc caaaaaccac ctcgtcccct tcggcgaata
     2341 caaaccgctg cctttcctga ccacgccgct ttacaaaatg atggatatgc ccctttccga
     2401 cttccgcaaa ggcggcggca agcaatccgc cctgctgatg aaaaaccaaa aaatcgcctt
     2461 caacatctgt tacgaagacg gattcggcga cgaactgatt gccgccgcaa aagatgccac
     2521 cctgcttgcc aatgccagca atatggcgtg gtacggaaaa tccaacgcca tgtaccagca
     2581 cctccaacaa tcgcaggcgc gggctatgga actcggacgc tatatggtcc gcgccaccaa
     2641 caccggcgca accgccatca tctcccccaa aggcaacatc atcgcccaag cccaacccga
     2701 tacggaaacc gtattggaag gacacatcaa aggctatgtc ggcgaaacgc cctatatgaa
     2761 aaccggcagt tcatggtggt tgatgggcat attggcccta gccgcactga ttcttttcat
     2821 cttccgaaac aaagaacact gatacctgca cagcccgtcc ggacgggctg tcttttttca
     2881 aaacggctat ttacatacaa ccacgtatgt aatataattc caaaatcgaa ttgagccatc
     2941 aggtttacaa aatcaatcaa acttgcctta caaaattttt cctgtctttc aaaccttagc
     3001 gcatatctcc tgccaaatat acaaaataca agccaagcag tgatatactg catcccaaca
     3061 cagcaaaccg ccgatacgca gtttcagccg aaaggaaatg tcccaaatga ataacgcttt
     3121 cgcattaccc gccatccaaa gcggcaacgg cagcctcgaa caatacatcc acaccgtcaa
     3181 cagaattccc atgctgaccc aagaggaaga aacacgcctt gccgaacgcc aacacaaagg
     3241 cgacctcaac gccgccaaac aactcatcct gtcccacctg cgcgtcgtcg tttccatcgc
     3301 gcgcggctat gacggctacg ggctgaatca ggcagacctg attcaggaag gcaatatcgg
     3361 actgatgaag gcggtcaaac gctacgagcc cggtagaggc gcgcgtctgt tttcatttgc
     3421 cgtacactgg attaaagccg aaatccacga gtttattttg agaaactggc gtttggttcg
     3481 cgttgccacc accaaaccgc aacgcaagct gtttttcaac ctgcgcagca tgcgtaaaaa
     3541 cctcaacgcc ctgtctccca aagaggcaca agacatcgcc gacgatttgg gtgtcaaact
     3601 ttccgaagtt ctggaaatgg aacagcgaat gacgggacac gacatcgcca tcatggcaga
     3661 caacagcgac gacgaggata gtttcgcccc catcgactgg cttgccgacc acgattccga
     3721 accgagccga caactatcga aacaggcgca ttacgccctg caaacagaag gtttacaaaa
     3781 cgctttggcg caactggatg acaggagccg ccgtattgtg gaaagccgct ggcttcaaga
     3841 cgacggcggg ctgacgctgc accagcttgc cgccgaatac ggcgtatctg ccgaacgcat
     3901 ccgccaaatc gaagcaaaag ccatgcagaa actgcgtggt ttcctgaccg aagaagccga
     3961 agcggtttga aatatacgca ataaaatacc cgacaaattg ttttgtcggg tattttattt
     4021 ggtaaacatc aatgcggttt gtgcgtcgtg ctgtacacga ttttatccgt ccacgccgtt
     4081 gcaattgccg aaatcaaaaa gcagacatgc accagacact gccgcatcag ctgcttttcc
     4141 gacagattgg cggaattgat atagtggatt aacaaaaatc aggacaaggc ggcgaagccg
     4201 cagacagtac aaat
//
LOCUS       DAWGRZ010000166         4191 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000166 DAWGRZ010000000
VERSION     DAWGRZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.166"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(91..465)
                     /locus_tag="WHT44_001773"
     CDS             complement(91..465)
                     /locus_tag="WHT44_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533919.1"
                     /translation="MIIQNEFNLYPSNMLPERFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNSRVIVINLDN
                     IENHEFFNSFEDWLELAIKDTW"
     gene            complement(638..994)
                     /locus_tag="WHT44_001774"
     CDS             complement(638..994)
                     /locus_tag="WHT44_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ2533920.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITENQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(1137..1481)
                     /locus_tag="WHT44_001775"
     CDS             complement(1137..1481)
                     /locus_tag="WHT44_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533921.1"
                     /translation="MNLENYENILIKLLFYHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDLLPD
                     NMIINNFFEIND"
     gene            complement(1640..2041)
                     /locus_tag="WHT44_001776"
     CDS             complement(1640..2041)
                     /locus_tag="WHT44_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533922.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            complement(2045..2785)
                     /locus_tag="WHT44_001777"
                     /pseudo
     CDS             complement(2045..2785)
                     /locus_tag="WHT44_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(2855..3172)
                     /locus_tag="WHT44_001778"
     CDS             complement(2855..3172)
                     /locus_tag="WHT44_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533923.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(3169..3771)
                     /locus_tag="WHT44_001779"
                     /pseudo
     CDS             complement(3169..3771)
                     /locus_tag="WHT44_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253822.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(4002..>4191)
                     /locus_tag="WHT44_001780"
     CDS             complement(4002..>4191)
                     /locus_tag="WHT44_001780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533924.1"
                     /translation="NSRYEPCIHINIRLITAEFDEIRQKLIVRFYLDREVTDDGKEDM
                     ETARTELLPGGYASSLVV"
BASE COUNT         1244 a          821 c          637 g         1489 t
ORIGIN      
        1 ataaaagttc ttgttccatt ttaaaacaat tagtaaaatc gagtttatcc tagttgtcca
       61 gggcagcctc atctctttat agatagctgc ttaccaagta tccttaattg ctaattcaag
      121 ccaatcttca aaactattaa aaaattcatg gttttctata ttatctaaat taatgacaat
      181 aaccctagaa tttcctgtta catcattacc atcaaaataa gcttcccatt ctccattact
      241 agcgaatggg ataagattta aatcaggcag gatagaattt ctaaatatat aagctacttc
      301 tgccccttct gttccataat tctcaaacca ccagtgaaaa ttatgtggct gaatataagg
      361 tattaaagat gtatcgttag agatacgaac atacttttca ggataacaaa acctttcagg
      421 aagcatatta ctaggatata aattaaattc attttgaata atcatttcgt taccttagga
      481 tcataaatac taccattagg aaactgggta gaccaatttg gtggtgctag ttcattttca
      541 atttgttttt taattctaaa ttgaatagct tctccccatg tttgcggcat ctaatgctgc
      601 ttgctctgca tcaatattac taaaaattgg attagtctca aaaatgaaaa attactgttt
      661 tttcattttt tatagcatat tcaagtacat gaataatatt tttcactaag ggctctgtag
      721 aaaaatctga attttgtaat ttttctttta cagccaaaat actttcctta tctattagcc
      781 aatccccctc acttggtcca aataaccaat tatcggtaat ttcagaaaca agtctccata
      841 atccacgatt ttgatattca ataatttgat tttccgtaat ttctacaact gtttcagatt
      901 gaatgttttt ataagcccaa tcaatcattt cagtatattc actaattgaa acattgtctt
      961 ttaaacagaa aattaacagg ttgtgttcat tcatttgtct aagttcctga tatgagtttg
     1021 ttgttgtaaa taatcttttg cttgcttacc ttgtaaatta aaggattaac cgcaccttgg
     1081 atgatgccgc gaatatcatc catacggtta ccccaccaat ttttattggt gtatcgttaa
     1141 tcatttattt caaaaaagtt atttattatc atattatcag ggagcaggtc gttaaaaaaa
     1201 accccctcaa attccattaa attagtattt atccagcaca taaacgaacc aaagcgttct
     1261 ttccctgagt gtaaatattt aaaattaaac tcaaaataag tttttttgct tagaaaattt
     1321 aatggttttt ctatagtgaa aaaaaagccc ttactcgttt tcgttcgaga tactatttta
     1381 aataaatttt tattttcaat aaaataagaa tattcattta ctaagttgtt atgataaaaa
     1441 agtaatttta ttaaaatgtt ttcataattt tctaggttca tttctagctc aatttaaaat
     1501 gatttcattg ttgccgtacc aatgcgaaat ttaccatcaa caaaatttcc cttcactgat
     1561 acaggtacac catcaacttg ggtagtttta ttttcaggct gcctgaaaga aagataggca
     1621 acttattact catcttacgc tagccctcaa taattgaata cgttgcacta gaaccaatta
     1681 tttctatgaa tgtttgctca aacataaaaa tatagtattt aaagaattct ctattcaaag
     1741 gaaaataact ttctttcaat atccattccg cataacactc atcaagaagt tcaaaaaaag
     1801 attgaccacc attttcatga agcaaagctc tatctgcatc ttccgaaatt aaatccataa
     1861 aagtatctat aaaatacgat tcatcaacaa ccctaaaatg aataacatct tcaataaaaa
     1921 tttttatggt tttatccgta tttctataac caactgctat aacttctact ttatcagagt
     1981 ttattgctgc attatcaaaa tagaaatagc cagttgggaa atctaagctt tttaatttca
     2041 taatttatcg tgtatttcca tatctgattt ttatttttcg gttattacta attgttattt
     2101 caagtgttgg ttctccacca ctactatcat tacgagcatt aaccgcacgc ccatcaggta
     2161 aattgcccgt tatcgttcca ttaggacgtg tttgtacatt tcgaacattc agactattaa
     2221 aatcatttag tgcgtcattg taatccccca ctttatcata ctgaacagcg ggaccttttg
     2281 ttttactcct ttgagtagta ttgctcaaaa tgtctttgat ttgtaaatca ctgcgcgcct
     2341 taccggcttc tatgcgagct gccacactac tcgggctata ggggtcgcta tcaatcgcag
     2401 gattaccacc gctggttttt tgtccgctag cctgtttccc aacctgctgt actgtatcgc
     2461 caatttcatc taccgtacct aacgccttag tctgcatatg gcgaacagca ggttttttat
     2521 agccggtatt tttaacccaa tcccatttgg tcgggttaag ttctactttt ttacctctcc
     2581 aaaccgtacc tgcggcctct gctacggcaa gggcagtttg ggctgttgct gttatattcg
     2641 gatgggcatc agcccattgt ctggcggaat tgatgccgtc ttttaccgca aaagcactgt
     2701 cctgtaatat gctcgcggca gcaagttgtg cttccgggct taattttcct gaattattaa
     2761 taccttgtag agtctgctgc gcagagatgt agcaactgta tttttacccc gtcgggtaaa
     2821 aatacagttg ctacggctgc tgggaaagca gttactattt tcccagtggc tcaaaattaa
     2881 ttgtttcagc agatattttt ataataatcc ctacatcaat aataatttca ctataaaatt
     2941 ggtatttata tagctctatt tcgggtataa acaagggaaa caagctatct tccttatcct
     3001 cataatctac gaaattattt gtatctaatt taaaattcaa aatattactg catttgatat
     3061 atccgaaata attaccttca attgtatctg taaaagataa tgtaagggta tttccattat
     3121 cggaaaagat tatttcataa aggcttacat tgtgaaaaat attttttttc atttgacaaa
     3181 atatccagaa tgatgatgat tatttggcaa tggattgcct tcataatctt gtcgttgcgg
     3241 atattttccc tgtacaccat cggttcgcgg cactctttgg gttcttataa cctctccatt
     3301 tggacgaata tctattttca cgccatcagg acctttccat gtttcatatt gagcctcatc
     3361 tgaaccagta cgtctaaaac cttgttcttg caatgcttga cgtaattcat ctcgcgtagt
     3421 tggtctgata gatttaacaa ctggcttgtt tcctcctgcc atctcaccgt ccaacgtctg
     3481 catagggcgg gcagcaggtg ttttatagcc gttattttta acccaatcct atttggtcgg
     3541 gttaagttct acttttttac ctctccaaac cgtacctgcg gcctctgcta cggcaagggc
     3601 agtttgggct gttgcagtta tattcggatg ggcatcagcc cattgtctgg cggaattgat
     3661 accgtctttt accgcaaaag tactgtccta taatgcgctt gcggcagcaa gttgtgcttt
     3721 cggacttaaa tttcctaaat cattaatacc ttgtaaagtt tgctgcgcag agatgtaggt
     3781 cggatatttg tatccgacaa aaatatttga cgcatctatc gtttccgaaa tgccgctgtt
     3841 ggaaaatgtc ggattcgaga atccgaccta cggctatttc gtttaagtca taaatagcca
     3901 ttcatgttcc ttctgaattt gcttttgcta tccttagaac caatcatata aacattatca
     3961 tcattaatta atgttctaag tttatgaagc ggtcaaatct gtcaaactac cagagatgaa
     4021 gcatatcctc ctggaagtaa ttctgttcgt gctgtttcca tatcttcttt gccatcatca
     4081 gttacttctc tgtctaaata aaatcttacg ataagttttt gtctaatttc atcaaattct
     4141 gccgtaatta atcttatatt gatatgaata cacggttcat acctgctatt a
//
LOCUS       DAWGRZ010000167         4120 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000167 DAWGRZ010000000
VERSION     DAWGRZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.167"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..2383)
                     /locus_tag="WHT44_001781"
     CDS             complement(<1..2383)
                     /locus_tag="WHT44_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ2533925.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRILLSTTDISSENGK
                     IKIQSYGDQYYYARQSELYTFERRSYKTGKWYNRKHITEVKEHKNAKPDAVTLSASQG
                     IDIKSGGSIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYS
                     KAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARAD
                     AKIILEGIKSSIHTETVSSSKSTLWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYI
                     VDIPKGNLKTQIETLTKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEGMTPAAAA
                     VVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGAAAGTAAGTGVAAGTAATTGVAAGTS
                     AAAITTAAGKAALASLASQAAVSLINNKGDINHTLKELGKSSTVRQAATAAVTAGVLQ
                     GISGLNTQAAEAVSKHFHSPAAGKLTANLINSTAAASVHTAINGGSLKDNLGDAALGA
                     IVSTVHGEVASKIKFNLSEDYIAHKIAHAVAGCASAVANKGKCRDGAIGAAVGEMVGE
                     TLLDGRDVGKLSPQERQKVIAYSQIIAGSAVALVKGDVNTAANAATVAVENNSLLARR
                     RVNIRWTSRQELEHEYAILEIQAITNQIRRLDPKFNGIAIMRNPREPWTRHDVQTYRQ
                     YYNQLRESRGFAVDPIYRIRINNGNEFNRIMSSKYPYNELYVANPKSATGYFRVDSYN
                     PATEEIISRKFTQFSQIQESTGIGYIKEAVRKYSPGAVISNVPSTPTTIRGRKL"
     gene            complement(2528..2959)
                     /locus_tag="WHT44_001782"
     CDS             complement(2528..2959)
                     /locus_tag="WHT44_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533926.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQTNCSLHET"
     gene            complement(2975..>4120)
                     /locus_tag="WHT44_001783"
     CDS             complement(2975..>4120)
                     /locus_tag="WHT44_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HEZ2533927.1"
                     /translation="AAVGEIVGEALTNGKNPDTLTAKEREQILAYSKLVAGTVSGVVG
                     GDVNAAANAAEVAVKNNQLSDKEGREFDNEMTACAKQNNPQLCRKNTVKKYQNVADKR
                     LAASIAICTDISRSTECRTIRKQHLIDSRSLHSSWEAGLIGKDDEWYKLFSKSYTQAD
                     LALQSYHLNTAAKSWLQSGNTKPLSEWMSDQGYTLISGVNPRFIPIPRGFVKQNTPIT
                     NVKYPEGISFDTNLKRHLANADGFSQKQGIKGAHNRTNFMAELNSRGGRVKSETQTDI
                     EGITRIKYEIPTLDRTGKPDGGFKEISSIKTVYNPKKFSDDKILQMAQNAASQGYSKA
                     SKIAQNERTKSISERKNVIQFSETFDGIKFRSYFDVNTGRITNIHPE"
BASE COUNT          910 a          911 c          929 g         1370 t
ORIGIN      
        1 caagctttct tcctcttatc gtagtaggtg tacttggaac attggaaatg acagcaccag
       61 ggctatattt tctaacagcc tccttgatat aaccaatccc cgtactttct tggatttgag
      121 aaaattgggt aaattttctt gaaataattt cctctgtcgc aggattatac gaatctaccc
      181 taaaataccc cgtcgccgat ttaggattgg ctacataaag ctcattataa gggtattttg
      241 atgacatgat acggttaaat tcattgccgt tgtttatcct gattctataa attgggtcaa
      301 cagcaaagcc tctggattcc cttaattgat tataatattg cctgtatgtt tgtacatcat
      361 gtcttgtcca cggctctcta ggattcctca taatagcaat cccgttaaat ttcggatcca
      421 gccttcggat ttgattggta atggcctgga tttcaagaat ggcatattca tgttccaatt
      481 cttgtcgcga agtccaacgt atatttaccc tcctgcgagc taaaagacta ttattctcca
      541 ctgccacagt agccgcattc gccgccgtat tcacatcccc tttaaccaat gccactgcgc
      601 tgcctgcgat aatctgcgag taggctatga ctttttggcg ttcttggggt gacagtttgc
      661 ctacatcgcg tccgtccaac agggtttctc ccaccatctc gccgactgcc gcgccgattg
      721 cgccgtcccg acatttgcct ttatttgcta ccgccgatgc acagcctgct acggcatggg
      781 ctatcttgtg ggcaatgtag tcttcgctga gattaaattt gattttgctc gctacttctc
      841 cgtgtacggt actgactatc gcacccagtg cggcatcgcc caagttgtct ttcaggctgc
      901 cgccgttgat ggcggtatgg acacttgcgg cagcggtgct gttgatcagg ttagcggtca
      961 gtttgcctgc tgcgggactg tgaaaatgtt tgctgacggc ttcggctgct tgggtgttca
     1021 gcccgcttat gccctgcagt acgcctgcgg ttacggcggc ggtggcggcc tgtctgacgg
     1081 tgctgctttt gcccagttct ttcagggtat ggtttatgtc tcctttgttg ttgatgaggg
     1141 aaactgcggc ttggctggcg agactggcca gtgcggcttt gcctgcggct gtggtgatag
     1201 ctgcagctga tgtgcctgct gctactccgg ttgtggctgc cgttcctgct gctactccag
     1261 ttccggctgc cgttcctgct gcggctcctc ccgctcctgc gcctgccgcg cccgccgttc
     1321 cggcggctgc cggggcggac agtgcgccgt aggtcaatac ggttacgacg ataacgacga
     1381 cagctgctgc tgcgggtgtc atgccctcct gtttgtagtc ccatttatcg taagcaagct
     1441 gcacctgatt ccagttgatg tttttcgcaa cttgaagttg tttcaaataa gcatactcgg
     1501 gctgcttggt gagggtttcg atttgggttt tcagattgcc tttcggaata tcgacaatgt
     1561 aaccgccggg tgcggacagt acgggcgcaa cgggaccggt gaaactcggc aattgcaagg
     1621 tttcgatgtt actgccccgt cctgcctgtt tttgccatag agtagatttg ctgctgctca
     1681 cggtttctgt gtggatgctg cttttgatcc cttcgaggat aatcttggca tcggcccgtg
     1741 cctgctcgcc tacgcctgcg cgtatggttg cgccacccag tgtggtttca aactgtgtgc
     1801 cttgcagttt ggtatcccaa cctgattgca gattggcaga ttctgcaact acccttgagg
     1861 gcagcgcggt tttcatgact tgggtggtgg tgtcgtgtgc tttgctgtag ctgatgccga
     1921 gaaatctgcg ccttttttgg ctgtcaagtt tgtcgtagtt gagctcttct acggcataga
     1981 gtgtcaattt ccgcccggct tcgatgttaa tgctgccttt gggggcatcg aatgcggtgg
     2041 cgtaggcgtc gatgctgcca ccagatttga tgtcgatgcc ttgggatgcg ctgagggtta
     2101 ctgcgtcggg cttggcgttt ttgtgttctt tgacttcggt aatgtgtttg cggttgtacc
     2161 atttgccagt tttgtagctg cggcgttcaa aggtatagag ttcgctctgt ctcgcatagt
     2221 aatattggtc accgtaagat tgaattttga ttttgccgtt ttccgaactg atatccgtgg
     2281 tgctgagcag gatgcgactg ttctcattgg catacggtgc gctaatgctc acaccggttt
     2341 tacccgaaag ttctgcagca agcgtagggg gtgtcttcag cacgcacgcg tctgggcagt
     2401 atatagggaa tctgaatatt tacttgcata acaaatgccg tctgaaaaat tgtgagcttt
     2461 tcagacggca ttgagccgta aatcatggaa cgcgtgcgtg ctgaagcaca caccttacgc
     2521 atggatttta ggtttcatgc aggctacagt ttgtttgaga atatgttttt tgtatttctt
     2581 tacacttttt ttgatattca gggtgagcta ttaagaaatc agatattgca atattaaagt
     2641 aatgatatgc taaattaata ggtataatta attcttcagg attgaaatca tctccaactg
     2701 aaaaataaat agaaggagct ttatcaatat actcaaatcc atttacctct tcataatctc
     2761 caatccatct ggtagcatct attttttcat attgagcacc tattactcca aaatgtccgg
     2821 tagttgtaat atttttaaaa cactctaaaa gcgtatcttt agtttcaaat tggtaaattg
     2881 tttcaaaaaa aatcgaaata acaaaatggt tgttatttat agatttttta tttaagttta
     2941 gaaaaatatt atttttcata atttttcctt taaattattc tgggtgaatg tttgtaattc
     3001 ttcctgtatt tacatcaaaa tatgatctaa atttgattcc gtcaaaggtt tctgagaatt
     3061 gaatgacatt ttttctttcc gatattgatt tagttctttc attttgagca attttagagg
     3121 cttttgaata tccttgtgaa gcagcatttt gagccatttg aagtatttta tcatcagaaa
     3181 attttttagg attataaaca gtttttatac ttgaaatttc cttaaatcca ccatcaggtt
     3241 tacctgtcct gtctagtgta ggaatctcat atttaattcg ggtaatgcct tcaatatcag
     3301 tttgggtttc agattttacg cgtcctcctc gtgaatttag ttctgccata aaattggtgc
     3361 ggttatgggc tcctttaatg ccctgttttt gactaaaacc atcagcattt gccagatgtc
     3421 tttttaggtt tgtatcgaaa ctgatgcctt ccgggtattt gacattagta ataggtgtat
     3481 tttgttttac aaaccctctt ggtattggaa tgaatctagg attaactcct gaaataagtg
     3541 tataaccttg gtcggacatc cattcggata aaggctttgt attgcccgat tgaagccaag
     3601 atttagcagc agtattcaaa tgataagact gtaaagccaa atctgcttgg gtgtaagatt
     3661 tgctgaataa tttataccat tcatcatctt taccaattag acctgcttcc caagatgaat
     3721 gaaggcttct actatcgatc aaatgttgtt ttctgattgt tctacattca gtactacggg
     3781 atatatccgt acatattgca atcgaagcag caagtctttt atcagcaaca ttttgatact
     3841 tttttacagt attttttctg cacagttgag gattattctg tttggcgcat gcagtcattt
     3901 cgttatcaaa ttctctaccc tctttgtcgc taagctgatt atttttcacc gctacctcag
     3961 ccgcattcgc cgccgcattt acatcgccgc cgaccacacc gcttaccgta ccggcaacca
     4021 gtttgctgta tgccaaaatc tgttcgcgtt ctttagctgt caaagtgtca ggatttttgc
     4081 cgtttgtcaa agcctccccg actatctcgc ccacagccgc
//
LOCUS       DAWGRZ010000168         4102 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000168 DAWGRZ010000000
VERSION     DAWGRZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.168"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..332)
                     /locus_tag="WHT44_001784"
     CDS             complement(<1..332)
                     /locus_tag="WHT44_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ2533928.1"
                     /translation="MFSLYREDSLISRLNRDGYLTSPSADFLELLSLCRDIHALTDGA
                     FDPTVQALWKLYADYFAARPDAETPPPQNSVDEALKRVGFGKVSFDDREIRFAEKGMG
                     LSLNGIAQG"
     gene            complement(313..594)
                     /locus_tag="WHT44_001785"
     CDS             complement(313..594)
                     /locus_tag="WHT44_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nosX protein required for nitrous oxide
                     reduction"
                     /protein_id="HEZ2533929.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNGDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRRAADLVNKVLAEVARLEKIVQPLP"
     gene            complement(603..1397)
                     /locus_tag="WHT44_001786"
     CDS             complement(603..1397)
                     /locus_tag="WHT44_001786"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ2533930.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVLEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            1781..3115
                     /gene="gdhA"
                     /locus_tag="WHT44_001787"
     CDS             1781..3115
                     /gene="gdhA"
                     /locus_tag="WHT44_001787"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ2533931.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFEKQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            complement(3250..4029)
                     /locus_tag="WHT44_001788"
     CDS             complement(3250..4029)
                     /locus_tag="WHT44_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ2533932.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSILKDREYQSRNRHADTLAQNDLKRVQDWAV"
BASE COUNT         1062 a         1197 c          946 g          897 t
ORIGIN      
        1 ccttgcgcga tgccgttcaa ggacaaaccc ataccctttt cggcaaaacg gatttcacgg
       61 tcgtcaaacg agactttgcc gaagccgacc cgtttcaggg cttcgtccac gctgttttga
      121 ggaggcggcg tttccgcatc gggacgggcg gcaaaataat cggcatacag tttccacaac
      181 gcctgcactg tcggatcgaa cgcgccgtcc gtcagcgcgt gaatatcgcg gcacaggctc
      241 aacagttcca aaaaatccgc cgacggcgaa gtcagataac cgtccctgtt caggcggctg
      301 atcaggctgt cttcacggta aaggctgaac aattttttcc aaacgcgcca cttccgccaa
      361 aaccttgttg accaaatccg ccgcacgcct gtcgtccaca ccgaacagac ggagctccgc
      421 accggaaccc agtgcgacac ctttccagaa aaacacattt tcattgcgtt tttcatcccc
      481 gttgcgtttt tcatcatcgg cggcaaaagg attcggcagg aaagaaaccg ccgcgcccgc
      541 cgccgcaacg gcggcaaccg tcagaaaacg cctgcgcccg aaatgcctgc ccataccgcc
      601 tcctaaaccg acactgccgc cttgatatgc ggatgagggt cgtaaccttc caactcgaaa
      661 tcttcaaact tgaaggaaaa caaatctttg acttcaggat tgattttcat cactggcaag
      721 gcgcgcggtt cgcgttccaa ctgcaatgcg gcctgctcga aatggttgcg gtacaaatgc
      781 gcgtcgccaa acgtatggac aaactcgccc gcctccaatc cgcacacttg cgccatcatc
      841 atggtcaaca atgcgtagct ggcaatatta aacggcacac caaggaaaat atctgcacta
      901 cgctggtaaa gctggcagga cagtttgccg tcggcaacgt aaaactgaaa cagcgcgtgg
      961 cagggcggca aggccatttc atcgaccaaa gccggattcc acgccgatac aatcaggcgg
     1021 cgcgagtcgg gattcttctt gatttgttcc agcacattgg cgatttggtc gatatgcctg
     1081 ccgtcgggcg cgggccagtt acgccactgg tagccgtaaa ccgggcctaa gtcgccgttt
     1141 tcgtccgccc actcgtccca aatggaaaca ttgttgtcct ttaggtattt gatattggta
     1201 tcgcctttga gaaaccaaag cagctcgtgg ataatcgaac gcagatgcag ctttttggtc
     1261 gtcagcagcg gaaaaccttt gcccaagtca aaacgcatct gataaccgaa tacggagcgc
     1321 gtacccgtac cggtgcggtc tgatttgtcc gtaccgttgt cgaggacgtg gcgcatcaag
     1381 tccaaatagg ctttcatagc agtctttcat caaattaaac ggcgcatatt gtaacatttc
     1441 cggataatgc ccaaaacacg gatacaggca ggcaggattg ttggcaattt cagtcctttt
     1501 ccacagtaaa acccggtggg aaaacaaaat taccttgatt ggaatcaaaa aatctagttt
     1561 aattacttag aataaaattt caataatatt tttatttacg aaattaattt atgattattg
     1621 attaattatc ggcaacaaac atcaaacatc gaaaatatgg aaaaaataat gtcaacaatt
     1681 tttgccaaat cgggcttggc atcagaaaaa aataggttta tattcccacc tacaaatttg
     1741 ttttcccatt agtacactat caaccaaaag gagtatccga atgactgacc tgaacaccct
     1801 gtttgccaac ctcaaacaac gcaatcccaa tcaggagccg ttccatcagg cggttgaaga
     1861 agtcttcatg agtctcgatc cgtttttggc aaaaaatccg aaatacaccc agcaaagcct
     1921 gctggaacgc atcgtcgaac ccgaacgcgt cgtgatgttc cgcgtaacct ggcaggacga
     1981 taaagggcaa gtccaagtca accggggcta ccgcgtgcaa atgagttccg ccatcggtcc
     2041 ttacaaaggc ggcctgcgct tccatccgac cgtcgatttg ggcgtattga aattcctcgc
     2101 ttttgaacaa gtgttcaaaa acgccttgac caccctgcct atgggcggcg gcaaaggcgg
     2161 ttccgacttc gaccccaaag gcaaatccga tgccgaagta atgcgcttct gccaagcctt
     2221 tatgaccgaa ctctaccgcc acatcggcgc ggacaccgat gttccggccg gcgacatcgg
     2281 cgtaggcggg cgcgaaatcg gctacctgtt cggacaatac aaaaaaatcc gcaacgagtt
     2341 ttcttccgtc ctgaccggca aaggtttgga atggggcggc agcctcatcc gtcccgaagc
     2401 gaccggctac ggctgcgtct atttcgccca agcgatgctg caaacccgca acgatagttt
     2461 tgaaggcaaa cgcgtcctga tttccggctc cggcaatgtg gcgcaatacg ccgccgaaaa
     2521 agccatccaa ctgggtgcga aagtactgac cgtttccgac tccaacggct tcgtcctctt
     2581 ccccgacagc ggtatgaccg aagcgcaact cgccgccttg atcgaattga aagaagtccg
     2641 ccgcgaacgc gttgccacct acgccaaaga gcaaggtctg caatactttg aaaaacaaaa
     2701 accgtggggc gtcgccgccg aaatcgccct gccctgcgcg acccagaacg aattggacga
     2761 agaagccgcc aaaaccctgt tggcaaacgg ctgctacgtc gttgccgaag gtgcgaatat
     2821 gccgtcgact ttgggcgcgg tcgagcaatt tatcaaagcc ggcatcctct acgccccggg
     2881 aaaagcctcc aatgccggcg gcgtggcaac ttcaggtttg gaaatgagcc aaaacgccat
     2941 ccgcctgtct tggactcgtg aagaagtcga ccaacgcctg ttcggcatca tgcaaagcat
     3001 ccacgaatcc tgtctgaaat acggcaaagt cggcgacaca gtaaactacg tcaatggtgc
     3061 gaacattgcc ggtttcgtca aagttgccga tgcgatgctg gcgcaaggct tctaagcaaa
     3121 cgccgccgct ccgcaaacaa aatgccgtcc gaaccgcaaa tgctgttcag acggcatttc
     3181 cttatccgcc cgttcaaatc gggtgagact accgatacat ctgaatatgc tatgccgtct
     3241 gaacggcatt cacaccgccc aatcctgcac gcgcttcaaa tcattttgcg ccaaagtatc
     3301 tgcgtggcgg ttacggctct gatattccct gtctttcaag atgctgctcg ccacataatt
     3361 caaatgtgcc tttgccgcct ccgaagcctc gcccggccgg cggtttgata ttgcctcata
     3421 caatacacgg tgctgcgcca tcagcttcgg acgcggatct tcttcctgat tcagataaat
     3481 aaggctgctg cgcgtctgcc ggtacagcat tttcaacaaa ccgcccgaca aatggctgaa
     3541 caacaaattg tgcgccgcat cggcaatcgt ctgatgaaag ctgacatcag cttcgctctg
     3601 atgttccaaa ttgccgcttt cgcacgcctc ctcaaacttt tcaagccaaa acccaatccg
     3661 cttcaaatcg gcatccgtgc ggcgttctgc cgccaatgcc gccatacagc cctcgatgtg
     3721 gcaactgaaa tcaaaaacat cctgttccca attggaatgc ttgcccaaaa gctcctgcca
     3781 actttgcaaa aaatcctgct gcggcttgac cgaaacataa taaccgtctc cctgcctcgc
     3841 ttccaaaacc tgacgggcga ccaaaacatt caatgccgac ctgaccgacg ggcgcgaaac
     3901 gccgaactct tccgccaaaa cgcgttcggg cggaatcttg cccccttccg cgtaaacccc
     3961 ttccgcaatg cgctcctcca ataccgacaa tacctgatcg ctgattttct gaggccttac
     4021 cagtttcatc actcctcctt tataaagatt ccctgcagaa cccttccgaa atatagtgga
     4081 ttaacaaaaa tcaggacaag gt
//
LOCUS       DAWGRZ010000169         4023 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000169 DAWGRZ010000000
VERSION     DAWGRZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.169"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..426
                     /locus_tag="WHT44_001789"
     CDS             <1..426
                     /locus_tag="WHT44_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hint domain-/BECR-fold containing toxin"
                     /protein_id="HEZ2533933.1"
                     /translation="DTLLSESGAKQTVQNITLKQQPLKAYNLTVADWHTYFVKGNRAE
                     TEGVWVHNDCPPRKTPSTPIYGNDSEAYAAAKELGYRKIKERTRNDAAIFKKGKSYIS
                     RDVDSHNGGAWKEASSPEKLNRKETRNGTFDKNLNRIGD"
     gene            432..839
                     /locus_tag="WHT44_001790"
     CDS             432..839
                     /locus_tag="WHT44_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ2533934.1"
                     /translation="MCEFKDFRRNIPCFKEYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRDIVIGIGTIIDFLMVQNWKLFEIKASTWLPKSVKINERYERFR
                     VMLRYIFTDLDVEDWKFFYFPIQHSKGRLRRKV"
     gene            861..1238
                     /locus_tag="WHT44_001791"
     CDS             861..1238
                     /locus_tag="WHT44_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ2533935.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRYVVIGIGTIIDFLMVPNWKLFEIKASPWLPDSVGIHERYERFT
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            1280..1759
                     /locus_tag="WHT44_001792"
                     /pseudo
     CDS             1280..1759
                     /locus_tag="WHT44_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
     gene            1740..2198
                     /locus_tag="WHT44_001793"
     CDS             1740..2198
                     /locus_tag="WHT44_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533936.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     RTEYYESPDSNLLISVKKKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHYPKSYGV
                     DKYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
     gene            2205..2261
                     /locus_tag="WHT44_001794"
                     /pseudo
     CDS             2205..2261
                     /locus_tag="WHT44_001794"
                     /inference="COORDINATES: protein motif:HMM:NF026864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2276..2653
                     /locus_tag="WHT44_001795"
     CDS             2276..2653
                     /locus_tag="WHT44_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ2533937.1"
                     /translation="MCELKNFRRNITCFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRYVVIGIGTIIDFLMVPNWKLFEIKASPWLPDSVGINERYERLK
                     TMLRYIFTEKDIVNVQFDYYNKK"
     gene            2717..3850
                     /locus_tag="WHT44_001796"
     CDS             2717..3850
                     /locus_tag="WHT44_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533938.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEVPE
                     NPNAFVAKLARLFRNADRAVVIVKESIRTEENLAGTVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGKEVWLDYHIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTVHGENYETTGEYRVVWQPDGSVFDAAGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFRKESNRIASDSRNSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADMQTYHAQQTWYLDGGRI
                     VREEKQGDRLPDFPLNLENLEKEVRRYAEAAARRSGGRRDLSH"
BASE COUNT         1308 a          684 c          938 g         1093 t
ORIGIN      
        1 gacaccctgc tttccgaaag cggtgcaaaa cagacggttc aaaacattac cctcaaacag
       61 cagccgctca aagcctacaa tctgaccgtt gccgactggc atacttactt cgtcaagggc
      121 aatcgggcgg aaacggaagg ggtttgggtt cataatgatt gtccgccaag aaaaacacca
      181 tctaccccca tatatgggaa tgattcagaa gcatatgcag cagcaaagga attgggatat
      241 agaaaaataa aagaaagaac gagaaatgat gctgctattt ttaaaaaagg aaagagttat
      301 atatcacgcg atgtagattc acataatgga ggtgcttgga aagaggcatc ttcacccgaa
      361 aaactgaaca gaaaagaaac aagaaacggt acatttgata aaaacctaaa tcgtattgga
      421 gattaattat tatgtgcgag ttcaaggatt ttagaagaaa catcccttgt tttaaggagt
      481 atgacgaaaa ttcatttatt ggcaaatggt atgatgacgg ggtgtgggat gatgaagaat
      541 attggaaatt ggagaatgat ttaattgagg ttagaaaaaa atatccttat ccgatggata
      601 taccaaggga catcgtgatt ggaatcggta ccattattga ttttttaatg gttcaaaatt
      661 ggaaactttt tgaaattaaa gcttctactt ggttgcctaa aagtgtcaaa attaatgagc
      721 gttatgaaag gttcagggta atgctccgtt atatttttac tgatctagat gtagaggact
      781 ggaaattttt ttactttcca atacaacata gtaaaggtag attgagacga aaagtttaat
      841 tatattggag atacattatt atgtgtgagt tcaaggatat tataagaaac gttccttatt
      901 ttgaggggta tgacgaaaat tcatttattg gcaaatggta tgatgacggg gtgtgggatg
      961 atgaagaata ttggaagttg gagaatgatt taatcgaggt tagaaaaaaa tatccttatc
     1021 cgatggacat accaagatat gttgtcattg gaatcggtac cattattgat ttcttaatgg
     1081 ttccaaattg gaaacttttt gaaattaaag cttccccttg gttgcctgat agtgtgggaa
     1141 ttcatgaacg ttatgaaaga ttcacaacga tgctccgtta tatttttacc gagaaagaca
     1201 tagtcaacgt gcgatttgat tattacaaca aaaaatagca aaacccggca atcaaaatgc
     1261 cgtctgaagc gatatccggc tttcagacgg catcggcaac agccaaatcc tgatttccaa
     1321 ccgcatccat ccgttttatt cggacggcaa atggattaaa gcggaagatt tgaaagcggg
     1381 aagcaggctg ttatccgaaa gcggcagaac ccaaaccgtc cgcaaaaccg ttgtcaaacc
     1441 aaaaccgctc aaagcctaca atctgaccgt tgccgattgg catacttact tcgtcaaggg
     1501 taatcaggcg gaaacggaag gggtttgggt tcataattct tgtccgccta aaagaacagg
     1561 aagctccaag aatgaaaaac atggagatgg cggtcgaagt caaatatcag cggaatcaaa
     1621 aattgctgaa ttaacaaata aaattattcc cggaatgtcc aaaaatgaac gcttaaagat
     1681 taagcaaaaa atcagaaata ttgccaaaaa tgccaatcga aaaacaaaag gagaagagca
     1741 tggtcgacgc ggtcgttaaa actcccgaat ttttaccttt tacttctgta ggaatattcc
     1801 gttttggtgc ggatataaca cagtacaaaa acatactgga aacttttatg tatgaacctc
     1861 ctgatgagtt tagaacagaa tattatgaat ctcccgactc aaatttactc atttccgtca
     1921 aaaaaaaaaa caaaattatt tcgatatttt gttatcagga gctatatttt atgggcgtta
     1981 atataatagg tctgaatttt gaagatttta aacagctttt ccattaccct aaatcatacg
     2041 gtgttgataa atattattta tcaaatgaat cttatccgac atatgtctat gagtttgatg
     2101 aaataggagt acaagcttgg gaagcaaaag ggaaaattgt tactatcatt gccgggggca
     2161 aagacaatta ttcaacagaa ccttactacg atgaataaca ttacagaaaa gaaacaagaa
     2221 acggtacatt tgataaaaac ctaaatcgta ttggagatta attattatgt gtattatgtg
     2281 tgagttaaaa aacttcagaa gaaatattac ttgttttgag gggtatgacg aaaattcatt
     2341 tattggcaaa tggtatgatg acggggtgtg ggatgatgaa gaatattgga agttggagaa
     2401 tgatttaatc gaggttagaa aaaaatatcc ttatccgatg gacataccaa gatatgttgt
     2461 cattggaatc ggtaccatta ttgatttctt aatggttcca aattggaaac tttttgaaat
     2521 taaagcttcc ccttggctac ctgatagcgt aggaattaat gaacgttatg aaaggcttaa
     2581 aacaatgctc cgttatattt ttaccgagaa agacatagtc aacgtgcaat ttgattatta
     2641 caacaaaaaa tagcaaaacc cggcaatcaa agctcgtctg atataatcga tatattctgt
     2701 taaacgaaaa ataaatatgt ttaaaaaatt caaaccggta ctgttgtcat tttttgcact
     2761 tgtatttgcc ttttggctgg gaacgggtat tgcctatgag attaatccgc gttggttttt
     2821 gagcgatacg gcaactgaag tacctgaaaa tccgaatgct tttgtggcga aacttgcccg
     2881 cctgttccga aatgccgaca gggcggttgt catcgtgaag gaatcgataa ggacggagga
     2941 aaatcttgcc ggaactgtgg atgacggtcc gttgcagtcg gagaaggatt atctcgcgct
     3001 cgctatccgg ctcagtcgtt tgaaagaaaa ggcgaaatgg tttcacgtaa cggagcagga
     3061 acatgggaaa gaggtttggc tggattacca tatcggcgag ggcggtttgg ttgcggtttc
     3121 gctttcgcaa cgctcgccgg aagcatttgt taatgccgaa tatctgtatc ggaacgatcg
     3181 tccgttttct gtaaatgtgt acggcggaac ggttcacggg gaaaattatg aaacgacagg
     3241 agaatatcgg gttgtttggc aaccagacgg ttcggtattt gatgcggcgg ggcgcgggaa
     3301 aatcggggaa gatgtttatg agcattgcct cgggtgttat cagatggccc aggtatattt
     3361 ggcgaaatac cgggatgtcg cgaatgacga gcagaaggtt tgggacttcc gcaaagagag
     3421 caaccgaatt gcgtcggact cgcgcaattc tgtgttttat cagaatatgc gggaattgat
     3481 gccccgaggg atgaaggcga acagtcttgt ggtcggctat gatgcggacg gtctgccgca
     3541 aaaagtctat tggagtttcg acaatggaaa aaaacgccag agtttcgaat attatttgaa
     3601 aaacggaaat ctttttattg cacaatcttc gacggtagca ttgaaagcgg atggcgtaac
     3661 ggcggatatg cagacctatc atgcgcaaca gacgtggtat ttggatggcg ggcggattgt
     3721 ccgcgaagag aaacagggag acagactgcc tgattttcct ttgaacttgg aaaatttgga
     3781 aaaagaggtg cgccgttatg cagaggctgc ggcgagacgt tcgggcggca ggcgcgacct
     3841 ttctcactga acgcaaatgc cgtctgaaac cggttttcag acggcattgg agagtcaaac
     3901 gctttttgca atgccgtata tgccgaatcc gattcgctac aaattgaaaa ccacaatcca
     3961 aaaaacggaa atacacatta aacgggtctt tttaccgacc gtatcatccc ataggttttc
     4021 cac
//
LOCUS       DAWGRZ010000170         4013 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000170 DAWGRZ010000000
VERSION     DAWGRZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.170"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(81..1331)
                     /locus_tag="WHT44_001797"
     CDS             complement(81..1331)
                     /locus_tag="WHT44_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ2533939.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     DAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKELFGAAYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVRKQVTALCNKYPVYGA"
     gene            complement(1398..1592)
                     /locus_tag="WHT44_001798"
     CDS             complement(1398..1592)
                     /locus_tag="WHT44_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533940.1"
                     /translation="MADKDVVGVGYLIDFAQTAENLPFNVLLLISLVLNKGNETLKTS
                     MLNKLPDNAKENLRIMGYLP"
     gene            complement(1813..3588)
                     /gene="ggt"
                     /locus_tag="WHT44_001799"
     CDS             complement(1813..3588)
                     /gene="ggt"
                     /locus_tag="WHT44_001799"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ2533941.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQNAISAPNLLNRFGSYELETGTTAVQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3756..3992)
                     /locus_tag="WHT44_001800"
     CDS             complement(3756..3992)
                     /locus_tag="WHT44_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEZ2533942.1"
                     /translation="MWIFSACSRGADPIGRPFAFVNILENPEIRSTLPLIASRPTFPQ
                     LWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
BASE COUNT         1009 a          953 c          938 g         1113 t
ORIGIN      
        1 tttttgcaaa ggtttccgcc tgaaacatta tgagatttca ggcggcattg gattgcttgg
       61 cggaatattt ttaaaaaggc ttacgcgccg taaacggggt atttattgca caaagcagtt
      121 acttgtttgc ggactttggc gaggttggct tcgtcttcgg ggttagacaa tacgtcggca
      181 accaagttcg ccaatacgcg cgcgtcggct tcgttaaaac cgcgtgtggt catggcagcg
      241 gagccgatgc ggatgccgga ggtaacgaag ggtttttccg gatcgttcgg aatggcgttt
      301 ttgttgacgg tgatgtgcgc tttgcccaaa gcggcttcgg cggctttgcc ggtaattttc
      361 atcggttgca ggtcaacgag gaaaacgtgg ctttcggtgc ggccggaaac gatgcgcaaa
      421 ccgcgtttaa ccaactcttc cgccatggcg gctgcattga ttttcacttg ttttgcgtat
      481 tgtttgaact cgggttgcaa tgcttcttta aacgccacgg ctttggcggc gataacgtgc
      541 atcagcggac cgccttgcag gcttgggaag atggaagagt tcaacgcttt ttcgtgggta
      601 ttgtcgcggc acaaaattac gccgccgcga ggaccgcgca gggttttgtg ggtggtggtg
      661 gtcacgaagt cgcagaacgg caccgggttg ggatattcgc cgccggcaac cagaccggca
      721 tagtgcgcca tatcgacaaa gaggtatgcg ccgactttat cggcgatttc gcggaatttt
      781 gcccagtcga tttgtaacgc gtaggcagac gcacccgcca caatcatttt gggtttgtgt
      841 tcgagcgcga ggcgttcgac ttcggcataa tcgagcactt cgttttcatc caaaccataa
      901 gtaacggcgt tgtagagttt gcctgagata ttaacgctcg cgccgtgggt caggtggccg
      961 ccgtgcgcta gagacatacc caaaatggtg tcgcctggtt ttaaaacgga agcgtacacg
     1021 gcttggttgg cttgcgagcc ggagtgcggt tggacgttgg cataggctgc gccaaacagt
     1081 tcttttacgc ggtcaatcgc caattgttcg acaatatcga cgtattcgca gccgccgtag
     1141 tagcgtttgc cggggtagcc ttcggcgtat ttgttggtca gctgggaacc ttgcgcgtcc
     1201 attacggcgc agctgacgta gttttcggaa gcaatcagct cgacgtggtc ttgctggcgt
     1261 tggtcttctt gggcaatggc tgctgccaaa tcggggtcgt attgtgcgag ggtaacgctt
     1321 tttgaaaaca tgttctcggc tcctttgtgt aatcagggta tcatgagtgt tttttgtata
     1381 aaaaaatatt tcaaaaccta aggcagatag cccataatgc gtaaattttc tttggcatta
     1441 tcaggtaatt tatttaacat gctggttttt agcgtctcat tacctttatt tagtacaaga
     1501 ctaatcaaaa gcaatacatt aaatggtaaa ttttcggcag tttgtgcaaa atcaatcaaa
     1561 taaccaacac caaccacatc tttatcagcc ataacccgct gatacaccac ttgctaagac
     1621 aactctcctg ccccaccatg tcttaaaatc agatgaatgg cttgtaatgc tgtgtttggt
     1681 gtctcacaaa ttgccaagat ctgctcagaa gcttgcttga tggcttgtat ttttgtttga
     1741 ttactcacaa tttcccccta ttttaataat taacttaaat gcggtcaaat tcacaaaata
     1801 caagctttac ctctaatcac ccatcactcg accttctcgg cgtggatcgg caccaccaac
     1861 cagcctgctt ggctcgataa taatggcttg aacacctgaa tttagctcac gcacatcagt
     1921 cttatagccc aaatcattta atgcttgttg ccactggacg gcggttgtac ccgtttctag
     1981 ttcatagcta ccaaagcgat ttaataaatt gggtgcactg atggcatttt ggatatccat
     2041 attccagtca ctatgtgcca caatcgtctt agcgacatag ccaatgatac ggctaccacc
     2101 tggggagccg attgccatat aaggcttgcc tgctttaaat acgatggttg gtgccattga
     2161 ggagcgtggt ctcttgccgg gctcgacacg attggcgacc tgtttgccct gctttattgg
     2221 ctcaaaacta aagtctgtca gctcattatt cagcaggtag ccatttgcca tcaaagttga
     2281 gccaaacgca ttttcaatgg aagtcgtcat tgatagcaca ttgcccgcct tatccacaat
     2341 tgatatatga ctggtagaag gtaactcaat cgcttgtgag gacacccact catgaataaa
     2401 atcgcctgca gatacgctag gcaatgcctt atccgactgc tcaagcagct ggctgcgatg
     2461 ttttaggtag tctttagaaa tcaactggcg aatgggtact ggtacaaaat cagggtcgcc
     2521 caaatataca tcacgatccg caaacgcaag cctagaagcg tcgcccaaga gacgtaaacc
     2581 ttcagcatca taccccacct gattgggtga aaattcattt aaaatcccca aaatctgacc
     2641 cacagcaatc ccacctgagc ttggtgcacc cataccgcat acttcataaa tacgataagt
     2701 cacacaaaca ggcgggcgtt ccaccacttg ataatcagat aaatcttgta aggataattg
     2761 accggggtta tccttagcat tttggacaac tgaaacgata ttttgggcat atttaccagt
     2821 atgcagagct tttgcacctt gagctgctaa cgcctgaaca ctgtcagcaa attctaaatt
     2881 tttcagcaag ctgcctgctt gtagcggcac accattcggc aaaaaataag cggctgtttt
     2941 tggatagcgt gccaaatgct gctgattttg ctcaaccgag atggcaagcc ttggcgacac
     3001 ctcaaagcct tgttttgcca agcggatcgg tgtatcaaat aattttcccc aaggcaatac
     3061 accgtatcgc tgatgtattg tctccatcag tttagggata gcaggcgtac ccaccgagcg
     3121 accaccgacc accgcttcca taaatttcaa tggttgacca tctttatcca aaaataattc
     3181 cggcgtcgca cgcatcggtg ccgtctcacg cccatcaaat gtggtcaatg ttttggcggt
     3241 attatcccaa tacaacacaa atgcaccacc gcccaagcct gacgactgtg gctctaccaa
     3301 gcttagtgtc gtctgcaccg ccaccatcgc atctgcagcg ctaccgcctt gctttaagat
     3361 atcatagcca gcttgtgttg ctaatggatt ggctgacgct accataaaat cacttgcaat
     3421 cacctgcttt tgttcggtca gtcccgttgc atgttcaggc gtgtgagcgt ctgcacctgt
     3481 gatgacagca gaatgagtat taaccttacc ttgattggca tggatgactt gacatccaga
     3541 gattgtcata gacattatca atgcagtcaa taaatatgtt ttagccacaa gcactccttc
     3601 gcctgagttt gattgatgat tcatacaagg catgctgatt attgtattta atatggctaa
     3661 ataattcaat ccaaactatc aatcttgacc atcaaaaaaa gaccgctaat gtcatcagca
     3721 gtcttttttg atatttattt taagatatta agtaatcaga cctttgggct atgctcttca
     3781 atgagtggtt ttagctcacc tgattggtac atttgtagga taatatcact accaccgatt
     3841 aactcaccat taacccaaag ctgtggaaag gttggccgac tggcgatgag tggtagagta
     3901 ctgcgaattt ctgggttttc taggatatta acaaaagcaa agggtctgcc aattgggtca
     3961 gcacctctac tgcacgcgct gaaaatccac attggggaaa ctggggcgtg cct
//
LOCUS       DAWGRZ010000171         3938 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000171 DAWGRZ010000000
VERSION     DAWGRZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.171"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(473..1513)
                     /gene="queA"
                     /locus_tag="WHT44_001801"
     CDS             complement(473..1513)
                     /gene="queA"
                     /locus_tag="WHT44_001801"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ2533943.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVTVGREGELFCLRFEGGETVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLHRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATARLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRHAVEREYRFFSYGD
                     AMVLGRNEGVVR"
     gene            1630..1887
                     /locus_tag="WHT44_001802"
     CDS             1630..1887
                     /locus_tag="WHT44_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ2533944.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            1869..2324
                     /locus_tag="WHT44_001803"
     CDS             1869..2324
                     /locus_tag="WHT44_001803"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ2533945.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            2436..3797
                     /gene="accC"
                     /locus_tag="WHT44_001804"
     CDS             2436..3797
                     /gene="accC"
                     /locus_tag="WHT44_001804"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ2533946.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAIYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGLIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
BASE COUNT          949 a         1192 c          961 g          836 t
ORIGIN      
        1 aatttatctt ataaacaaac aaaggcagcc tgaaagattg aatggtctgc acccctaagg
       61 ttggactaac caaccgacta aggtgcagat tattttttgt tgctttttca gcttttcgtt
      121 gggttagata ttcttgccca ctgttttcag gcagccttga atacaaaaaa atggcgtatg
      181 taatatgttt atacgaccaa aacggaatga attttaacgt attgcgcgtc atcaacaatg
      241 actgagtttc tcgcctctcg cgcctgaatc tatagtggat taacaaaaac cagtacagcg
      301 ttgcctcgcc ttgccgtact atttgtactg tctgcggctt cgttgccttg tcctgattta
      361 aatttaatcc actatatgtc atgcagttcc tttcattcaa atcaacaaaa gaatgccgtc
      421 cgaacgtccg ttcagacggc acttgtcttc ccacaataga cttgaggctg ttctaacgta
      481 ccaccccttc gttccgcccc aaaaccatcg catcgccgta gctgaagaaa cggtattcgc
      541 gttcgaccgc atgacgatac gcggcgcgga tatgacccat acccgaaaac gcgctgacca
      601 acatcagcag cgtcgatttc ggcaaatgaa aattggtaac cagtctgtcg acaacattaa
      661 aacggtagcc cggcgtgatg aaaatatcgg tgtcgccctg ccccgctttc agacgagccg
      721 tcgcacgcgc ggcagattcg agggcgcgca tggaagtcgt gccgaccgcc cagactttgt
      781 tcccccgggc ttttgccgcc tcaacggcgg cggcggtttc agacggcact tcaaaccatt
      841 cgctgtgcat tttgtgctct tcgattttgt ccacacgcac gggttggaac gttccggcac
      901 cgacgtgcag ggttacttct gcggttaccg cgcctttgtc tttcagacgg tgcaaaagtt
      961 cttccgtaaa atgcaggccc gccgtcggcg cggcgaccgc gccctgatat ttggcataaa
     1021 cggtttgata acggctgtcg tcatccgcat cggcggcgcg ttcgatataa ggcggcaggg
     1081 gcaggtgtcc gttctgttcc aaaagttcgt aaacggtctc tccgccttca aaacgcaggc
     1141 agaacagttc gccctcacgc ccgaccgtca cggcgcggat gccgccttca aacaccagcc
     1201 ccataccggg cttgggcgat ttggacgaac ggatgtgcgc cagtgcggta tggttgtcca
     1261 acacgcgctc aatcagggct tcgatcctgc cgccgctgtc tttctgccca aacagccgcg
     1321 ccttcatgac tttggtgttg ttgaacacca aaacgtcgcc cgcctcgaca taatccggca
     1381 aatcgccgaa cacccggtct tgcagcggca tatcgggcaa cgcaaccaaa aggcggctgc
     1441 tgccgcgcac ttcgggcgga tgctgggcaa tcagcttttc gggcagggta aaatcaaaat
     1501 ctgaaatatc catttttaca ctctcgttcg ggcaagccgc cattatacgc actttagcgc
     1561 tttttcagac ggcatctttg tccgaaaaac caacagatta gaataaacac tcttaacctg
     1621 gaacatcttg tgcgcaaaat caaacttcct gcacatttcc cccaaaaacc gccgtttttt
     1681 gatattttac tggacattta ccgacaactt cgggaaaata aacacattct cacggtcgtt
     1741 ttccaccaca ggaaaaccgt atccgaacac cattccgccc ggtttgcgcc gttgccgcaa
     1801 gccggctgtt ttctgaaaaa ccaacgcaac aacccgccgg aacaccggca gcctttaaag
     1861 gaacagaaat ggatttgcgc aaattaaaaa aactgattga tttggttgaa gaatcgggta
     1921 tcgccgaaat cgaagtaacc gaaggcgagg aaaaagtccg catcacccga accatcgccg
     1981 ccgcacccgt ttacgccgcg cccgtacctg ccgccgcgcc ggccgtaacg cctgccgccg
     2041 cacccgttgc ggcatccgcg cccgccgccg cacctgccgc ccgcgatttg tccgacgcgc
     2101 aaaaatcgcc tatggtcggc acgttctacc gcgcacccgg cccgaatgcc gcgccttttg
     2161 tcgaagtcgg ccaacaagtt aaagccggcg acacgctgtg catcatcgaa gcgatgaagc
     2221 tgatgaacga aatcgaagcc gaaaaatccg gcacggtcaa agaaattttg gtcgaaaacg
     2281 gtacgcccgt cgaattcggc gaaccgctct tcattatcgg ataatcctgt tttcagacgg
     2341 cataaacttc cgatgccgtc tgaaatgctt tcccccttca gcgttcccgc accctttttt
     2401 acggacgggt tgccggaacc gcaggaaagg tcatcatgct gaaaaaagtt ttaatcgcca
     2461 accgaggcga aatcgcatta cgcgtactcc gtgcctgccg cgaaatgggc attgccaccg
     2521 tcgccgtgca ttccgaggcc gacaaagaca gcctgcacgt caaactcgcc gacgaatccg
     2581 tgtgcatcgg ccctgccgct tccgcgcaaa gctaccttaa cgtccccgcc attatcgccg
     2641 ccgccgaagt aagctgcgcg gacgctgtcc atccgggtta cggtttcctt gccgaaaacg
     2701 ccgatttcgc cgaacaggtc gagcagtccg gctttacctt tatcggcccg aaacccgaca
     2761 ccatccgcct gatgggcgac aaagtctccg ccaaacacgc gatgatagcg gcaggcgtac
     2821 cctgcgtccc cggttctgac ggcgcattgc ccgacgacgg cgaagaaatc ctcaaaatcg
     2881 ccgataaagt cggttatccc gtcattatca aagcctctgg cggcggcggc ggccgcggta
     2941 tgcgcgtggt cgagaaaaaa gaagacctcc tccaatctgt cgaaatgacc aaagccgaag
     3001 caggcgcggc attcggcaac ccgatggttt acatggaacg ctatttgcaa cgtccgcgcc
     3061 acgtcgaaat ccaagtgatt gccgacgaac acggcaacgc catctacctt gccgagcgcg
     3121 actgttcgct gcaacgccgc caccaaaaag tcatcgagga agcaccggct ccgttcatca
     3181 ctgaaaaaga acgcgccaaa atcggcaacg cctgtgccga tgcctgcaaa cgcatcggct
     3241 accggggcgc gggtacgttt gagtttttat acgaagacgg cgaatttttc tttatcgaga
     3301 tgaacacgcg cgttcaggtc gagcatccgg ttaccgagct catcaccggc gtggacatcg
     3361 tgcaggagca actccgcatc gccgccggcc tgcctttgca atacaaacaa aaggatattc
     3421 aagtcgaagg ccacgcgttt gagtgccgta tcaacgccga agacccgtac aacttcattc
     3481 caagcccggg cctgattgaa agctgccacc tgcccggcgg cttcggtatc cgcgtggaca
     3541 gccacattta ccaaggctac cgcatcccac cgtactacga cagcctgatc ggcaaaatct
     3601 gcgtacacgg caaaacgcgt gaacaggcaa tggcgaaaat gcgcgtcgca ctcgccgagc
     3661 tggcggtaac cggcatcaaa accaatacgc cgcttcaccg cgacctgttc gccgatgcgg
     3721 gtttccaaaa aggcggcgtc agcatccact atttggaaca ctggctggaa gatcgcaaag
     3781 ccaaacagga caagtaaacc gccgccgata tgccgtctga agccgcccgt ccgcgttcag
     3841 acggcatttc ccttgccccg cgccgtctga aaccgatttc gatatagtgg attaacttta
     3901 aaccagtacg gcgttgcctc gccttagctc aaagagaa
//
LOCUS       DAWGRZ010000172         3917 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000172 DAWGRZ010000000
VERSION     DAWGRZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.172"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(38..604)
                     /locus_tag="WHT44_001805"
     CDS             complement(38..604)
                     /locus_tag="WHT44_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ2533947.1"
                     /translation="MGFYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWVAFVLLGGLGLKMMREGLSGEAEDVRESKRES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVMVAVGLTAGRALGVLF
                     GRCAEFAGGLVLIAIGTWTLLSHLGLIQ"
     gene            complement(724..924)
                     /locus_tag="WHT44_001806"
     CDS             complement(724..924)
                     /locus_tag="WHT44_001806"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533948.1"
                     /translation="MPSETLSDRFRRHSPIPPRRFSGRFLLNAKYPVIPTKVGIKDSG
                     LEKPFYPISFRTDNSGFPPARE"
     gene            complement(1000..2514)
                     /gene="katA"
                     /locus_tag="WHT44_001807"
     CDS             complement(1000..2514)
                     /gene="katA"
                     /locus_tag="WHT44_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ2533949.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQAGLL"
     gene            2862..3713
                     /locus_tag="WHT44_001808"
     CDS             2862..3713
                     /locus_tag="WHT44_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEZ2533950.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QEAACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLQHALTALKKFDPAGVAA
                     ADLNESLILQIERLGECAAKPAALHIVRNALDSIDGNRSQTLARIKKHLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAA
                     HKRKTAE"
BASE COUNT          920 a         1083 c         1021 g          893 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactatattt tccgacatca ttgaatcaaa cccaaatgcg
       61 acaagagcgt ccatgtgccg atggcaatca acaccaaacc tccggcaaat tccgcacacc
      121 tgccgaacaa tacgcccaaa gcccttcccg ccgtcagccc gaccgccacc atcaccgtcg
      181 tcgccatacc gatgattgcg gcggcaaagg cgatgtttac ctccataaac gccaagccca
      241 ccccgactat catggaatca atactggttc caaaagcagt caaaaccgtc atccataggc
      301 tttcccgttt gctttcgcgc acatcttccg cctcgccgga cagcccttcg cgcatcattt
      361 tcagacccag cccgcccagc aggacgaaag ccacccaatg gtcccattcg ctgataaacg
      421 gcttggcata aaaaccgcct acccagcctg ccagcggcgt gagcgcttca accgtgccga
      481 acaccaaagc cgttgccgca attttgcgcg gaggcattct gaccgccgca ccctttgcca
      541 atgcgacggc aaacgcatcc atcgacatcc ccagagcaat caagagcaaa gcataaaaac
      601 ccataccgca cccgtcctca aaaagggcgg attatagcaa aagcaaaaaa atgcaaaaat
      661 gccgcacgaa aacccgcatc ccgtcattcc cgcaaaaaca aaaaatcaaa aacagaaatc
      721 ccgtcattcc cgcgcaggcg ggaatccaga gttgtcggtg cggaaactta tcggataaaa
      781 cggtttctcc aaccccgagt ccttgattcc cactttcgtg ggaatgacgg gatattttgc
      841 gtttaataaa aaacgcccgc tgaaacggcg gggcgggatg ggggaatgcc gtctgaaacg
      901 gtcggacaat gtttcagacg gcatttttat gcccggttat ttccgatagc ggacggcgcg
      961 ggacaggatt tcttcaattt ccatccacat aatgccccct tacagcaaac cagcctgacc
     1021 cagtgcggga tcggtcgcgc gggcggcttg ggcatcttcg acagtcagtc caagggcttt
     1081 ggccacgcct tcgccgtatg ccgggtcgca acggtagcag ttgcggatat ggcggtattt
     1141 gatgaagtcg ggcgcgtcgc ccattgcggc ggcggtgttg ccgaacaatg cctgtttctg
     1201 cgcgtcgttc atcaggttga acagggcgcg cggttggctg aaatagtcgt catcgtcttg
     1261 gcggtagtcc cagtgtgccg cgtcgccgtt gattttcaaa ggcggttcgg cgaagtcggg
     1321 ttgttgctgc cattggccga agctgttggg ttcgtagtgc ggcaggctgc cgtagttgcc
     1381 gtcggcgcgg ccttgcccgt cgcgctggtt gctgtgaaca gggcaacgcg gacgattgac
     1441 gggaatttgg cggaagttta cgcccaaacg gtagcgttgt gcgtcggcgt aattgaacaa
     1501 acgcgcttgc agcattttat ctgggctggc gccgacaccg ggaacgaggt tgctcggtgc
     1561 gaaggcggat tgttccacat cggcgaagaa gttttcggga ttgcggttca actcgaattc
     1621 gcccacttca atcagcggat agtctttttt cggccaaact ttggtcaagt caaacggatg
     1681 ataaggtact ttttccgcgt ctgcttcagg catgacttgg atgtacatcg tccatttcgg
     1741 aaactcgccg cgttcgatgg cttcgtataa gtcgcgctga tggctttcgc ggtcgtcggc
     1801 gatgattttg gcggcttctt cgttggtcag gtttttaatg ccttgttggg tgcggaaatg
     1861 gaatttcacc caaaaacgct cgcctgcttc gttccagaag ctgtaggtat gcgaaccgaa
     1921 gccgtgcata tggcggtagc cggcggggat gccgcggtcg ctcatcacga tggtaacttg
     1981 gtgcagtgct tcgggcagca gcgtccagaa gtcccagttg tttgtggcag agcgcatatt
     2041 ggtgcgcggg tcgcgtttga cggctttgtt caggtcgggg aacttacgcg ggtcgcgcag
     2101 gaagaacacg ggcgtgttgt tgccgaccac atcccagttg ccttcttcgg tataaaattt
     2161 caaggcaaaa ccgcggatgt cgcgttctgc atcggctgcg ccgcgttcgc ctgccacggt
     2221 ggtgaaacgg gcgaacatct cggttttttt gccgacttcg ctgaagattt tggcgcgggt
     2281 gtatttggtg atgtcgtgcg ttacggtaaa cgtaccgaac gcgcccgaac ctttggcgtg
     2341 catacggcgt tcggggatga cttcgcgcac gaagtcggcg agtttttcat tcagccacaa
     2401 atcctgcgcc agcagagggc cgcgaggacc ggcggtcagg ctgttttgat tgtcggcaac
     2461 aggcgcgccg ttgttcatgg tcagatgggt tacagggcat ttggaggtag tcatcgctct
     2521 tgttcctttt ctcaggttgg tcaaatgggg gtaaacggct tacagtacga tttggcggaa
     2581 agcgtattcg taaccggttt cttgattgca ataaatttct tgaatcgaca ttttatttcc
     2641 cttttgtaaa aactatggat gcgactatac gccaagattt tcgctattaa aactatgaaa
     2701 tcgatttaat attattataa gcaatcggtt cttgattttc gtttgttttt tgttatcgaa
     2761 cggaatccga acccgctcat taaaaccatt tataatgcaa tgacgctttg cggcattttt
     2821 tgcgccgaca ggctgaaaat aacaattttc cccacattat catgacctta ctcggaataa
     2881 agctcaaaca gacccagcag ctcaaccagc ggctgcaaca atctttgcgc gtattgcaga
     2941 tgtcgggtat cgaacttgaa cgcgaggtcg aaaactggct gtcggacaac cccctgctcg
     3001 aacgcaaaga cacggatgaa ttttccgatg ccgagttcag ccattacact gcgcctgccc
     3061 gtcaaatcgg cggagacgaa ggcgaagata tgctgtccaa catcgccggc gagcaggatt
     3121 tcaagcaata cctgcacgcg caagtatgcg aacacccgct ttccgaccaa gaagccgcct
     3181 gtgtccacat ccttatcgat ttccttgacg agcagggtta tctgaccgac agcatcgaag
     3241 acatcctcga ccatacgccc ttagagtgga tgttggatga agcaatgctg caacacgcgc
     3301 tgaccgcatt gaaaaaattc gacccggcag gcgtggccgc cgccgatttg aacgaatcgc
     3361 tgatactgca gatagaaaga ttgggcgaat gtgctgccaa acccgccgcc ctgcatatcg
     3421 tccgaaacgc cctcgacagc attgacggca accgcagcca aaccctcgca cgaataaaaa
     3481 aacacctgcc ccaaaccgac agcggcacac tcgaagccgc actcgacctc attgcttcgc
     3541 tcaatccctt tcccgccgcc ggttttgcct cgtccacgcc cacgccgtat tctgacgagg
     3601 cgctcgccaa cctgctggct ttccgcggca tggaggtttc tcgccgcacc attgccaaat
     3661 acagagaatc ctttgagatt ccggcagcac acaaacgcaa aaccgcagaa taattgccga
     3721 ataatcttat aaagacaaca aaccaaaagc cggcatttct gcgaaagcgg gaatgccgaa
     3781 tccgtccgcg cggaaacctg catcccgtca ttcccgcgaa agagggaatc tagaaacgca
     3841 aagctgcaag aatttatcgg aaatgaccga aactcaacga acctggattc ccgctttcgc
     3901 gggaatgacg ggggttt
//
LOCUS       DAWGRZ010000173         3907 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000173 DAWGRZ010000000
VERSION     DAWGRZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.173"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            120..1025
                     /gene="glyQ"
                     /locus_tag="WHT44_001809"
     CDS             120..1025
                     /gene="glyQ"
                     /locus_tag="WHT44_001809"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ2533951.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1102..1511
                     /locus_tag="WHT44_001810"
                     /pseudo
     CDS             1102..1511
                     /locus_tag="WHT44_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
     gene            1532..3595
                     /locus_tag="WHT44_001811"
     CDS             1532..3595
                     /locus_tag="WHT44_001811"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ2533952.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAASVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQIGKPLGGLLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLVVLHGGDIVNVSVLGLQSGNKTLGHRFLSDGEITIENADSYAAQMREQGKVVA
                     SFAERKAAIQTVLEGQARRLNATAAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIQGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLTNVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEITEAVEQHYQPRFAGDNLPEGKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFN
                     SFPQGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3612..>3907
                     /locus_tag="WHT44_001812"
     CDS             3612..>3907
                     /locus_tag="WHT44_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ2533953.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLDILIVDDGSTDGTLAIAQRFQEQDGRIRILAQPRNSGLIPSLNIGLDELA"
BASE COUNT          991 a         1138 c          991 g          787 t
ORIGIN      
        1 aatttaatcc actatatcat gtatgccgtg ttttcaaatc agtctgtctt tgagataaac
       61 ctatccaaac agcaatggaa acagtaaaat aaacccttta ttttatcgaa tcataagcca
      121 tgcttacctt ccaacaaatc atcttcaaac tgcaaacatt ctgggcagac aaaggctgca
      181 ccgtcatcca acccttcgac atggaagtcg gtgccggcac atcccatccc gccacctgcc
      241 tgcgcgcact cggccccgag ccttggtttg ccgcctacgt ccaacccagc cgccgcccca
      301 aagacggccg ctacggcgac aaccccaacc gcctgcaaca ctattaccaa ttccaagtcg
      361 ccctcaagcc cgctcccgcc aatatccaag acctctatct cgactctctg cgcgaattgg
      421 gcatcgatcc caaagtccac gacatccgct ttgtcgaaga cgactgggaa aaccccaccc
      481 tcggcgcgtg gggtttgggc tgggaagtct ggctcaacgg catggaagta acccagttta
      541 cctatttcca acaagtcggc ggcatcgact gcacgcccgt actcggcgaa atcacctacg
      601 gcatcgaacg cttggcgatg tacttgcaag gtgtagaaaa cgtctacgac ctcgtttggg
      661 caaaaacgct cgacggcaac accgtcacct acggcgacgt gtaccaccaa aacgaagtcg
      721 agcaatccac ctacaacttc gaatacagcg atgccgactg gctgctgcgc cagttcaacg
      781 actacgaagc gcaagccaaa cgcctgcttg ccgaagaaaa cgccgccctc gccctgcctg
      841 cctacgagct tgtcctcaaa gcggggcata cgttcaacct tttagacgca cgcggcgcga
      901 tttccgtaac tgagcgggca acttatattg gacgtattcg tgcattgagc cgcgccgtgg
      961 cgcaaaaata tgttgaaagc cgcgaaaaac tgggcttccc tttaatgaag gcaaacgccg
     1021 cctaaggggg cgacgatttt ttacgttcgg gcggcacgat aaggcagccg tccggaagcc
     1081 attatatttt ttaggaaaat catgcctgtc tatttccgca ccccaagcag gaaacgcctg
     1141 cacaaaatga accgccgcca gcataaaaag ctgcatttgg gcgagtttca aacattgatt
     1201 ttcggcgttc aaggcagttt gcaccaagct gaggacgatg ccgaagcgtt cgaccgcttt
     1261 atggacgagc tgattgcgta tcttgcggca aacggctttg aaatggcggg gggggcgatt
     1321 gcggcgattt ttcgtgcctg tttcaaaaat ccggtgcccg aagcttggga gaaaccgaga
     1381 aaaaagccat tatctcttgg ctggcagacc gcgaagatgt tgccactttg tacgccaccc
     1441 cgctggttga cggtttttat ggggatgacg gctgggaaaa ttacagcgaa acctataaac
     1501 aggcagatta accaaattca aagaaagccg aatgatgacc caaacccttt taatcgaact
     1561 ccttaccgaa gaactcccgc ccaaagccct gaataatctg ggcaaccatt tcgccgcttc
     1621 cgttgccgaa ggcttggaaa aagcgcaact ggttgacggc gcagccgaat ttacggctta
     1681 tgcctcgccg cgccgtttgg cggttcaagt caaaaacgtc aaagccgttc aggctgatca
     1741 aaaaattgtg aaaaaaggcc ctgccgtagc gaatgccatg aaagacggtg cgccaaccaa
     1801 ggctttggaa ggtttcgcac gcggtgcggg cgcgaaaatc gaagacttga ccatcgtcca
     1861 cgacggcaag caggacgtgt acgcctacga atacgtccaa atcggcaaac cgttgggcgg
     1921 acttttggaa gacattatca atcaagcggt taagaagctg ccgattccca aagtaatgcg
     1981 ttggggcagc agcacgttta cctttgtgcg ccccgttcac gggctagttg tactgcacgg
     2041 cggcgacatt gtgaacgtca gcgttttggg tctgcaaagc ggcaacaaaa ccttgggaca
     2101 ccgcttcctc tccgacggcg aaattaccat tgaaaacgcc gacagctacg ccgcacaaat
     2161 gcgcgagcaa ggcaaagtcg tcgcttcgtt tgccgagcgc aaagccgcga ttcagacggt
     2221 attggaaggg caggcacgcc gtctgaacgc gaccgccgcc gccgatgaag ccttgttgga
     2281 cgaagtaacc gcgctggtcg aatggcctgt ggtattggaa gccggttttg aagaacactt
     2341 cctcgccgta ccgcaagaat gcctgattct gacgatgcag caaaaccaaa aatacttccc
     2401 gctgctcgac caaaacggca agctgatgaa ccgcttcctg ctggtgtcca atctgcaaac
     2461 cgaagatccg tcacacatca tccaaggcaa tgaacgtgtc ttgcgcgcgc gcctgtctga
     2521 tgccgagttc ttctacaaac aagaccaaaa agcgactttg gaaagccgcc tgcccaaact
     2581 gacgaacgtg gtttatcaca acaaaatcgg ttcgcaagcc gaacgcatcg aacgcctgca
     2641 aagcatcgcc gcccatatcg ccaaggcttt gggcgcggat gccgccgcag ccgaacgcgc
     2701 cgcgcgtttg gcaaaagccg acttagtaac cgaaatggtc ggcgagttcc ccgaactgca
     2761 aggcacgatg ggcaaatact acgcccgctt ggacggcgaa accgaagaaa ttaccgaagc
     2821 cgtcgagcaa cactaccaac cgcgttttgc cggcgacaac ctgcctgagg gcaaaattgc
     2881 cgccgccgtt gcgcttgccg acaaactgga aaccttggtc ggcatttggg gtatcggcct
     2941 gattccgacc ggcgacaaag acccatacgc cctgcgccgc gccgcattgg gtattttgcg
     3001 tatgctgatg cagtacggtt tggacgtaaa cgaactgatt cagacggcct tcaacagctt
     3061 cccgcaaggt ttgctcaacg aaaaaacgcc gtctgaaacc gccgacttca tgcaggcgcg
     3121 ccttgccgta ttgctgcaaa acgattatcc gcaagacatc gttgccgccg tacttgccaa
     3181 gcagccgcgc cgtttggacg atttgaccgc caaactgcag gctgtcgccg cgttcaaaca
     3241 actgcccgaa gccgccgcgc tcgccgctgc caacaaacgc gtgcaaaacc tgctgaaaaa
     3301 agccgatgcc gagttgggcg cggttaacga aagcttgctg caacaggacg aagaaaaagc
     3361 cctctttgcc gccgcacaag gcttgcagcc gaaaattgcc gccgccgttg ccgaaggcaa
     3421 tttccagacc gccttgtccg aactggcttc cgtcaaaccg caagtcgatg ccttcttcga
     3481 cggcgtgatg gtgatggcgg aagatgccgc cgtgaaacaa aaccgcctga acctgctgaa
     3541 ccgcttggca gaacaaatga acgcggtagc ggatatcgcg cttttgggcg agtaaccgtt
     3601 gtacagtcca aatgccgtct gaagccttca gacggcatcg tgcctatcgg gagaataaat
     3661 tgcagccttt agtcagcgta ttgatttgcg cctacaacgt agaaaaatat ttcgcccaat
     3721 cattagccgc cgtcgtgaat caaacttggc gcaacttgga tattttgatt gtcgatgacg
     3781 gctcgacgga cggtacgctt gccattgccc agcgtttcca agaacaggac ggccgcatcc
     3841 gcatcctcgc gcagccgcgc aattccggtc tgattccctc tttaaacatc gggctggatg
     3901 aattggc
//
LOCUS       DAWGRZ010000174         3859 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000174 DAWGRZ010000000
VERSION     DAWGRZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.174"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="WHT44_001813"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="WHT44_001813"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ2533954.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRIGMA
                     AAALNCRLKRCVLPDEVFAECEQKLLHPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGKGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVFD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="WHT44_001814"
     CDS             1439..1924
                     /locus_tag="WHT44_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ2533955.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WHT44_001815"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WHT44_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ2533956.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMISGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIAGQLSG
                     GERGRLHLAKTLLSGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
BASE COUNT          794 a          946 c         1070 g         1049 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cctcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 ccgtcagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggc
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataat
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgcaga tgtcaaacct
      661 gaagacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggtgcg
      721 ataccgcgtt gggtgttgct gagcgcggct ttgcccgaat tggatgaggt atggctgaaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcgttgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtatc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc agatgaagtg
     1021 tttgccgaat gcgaacaaaa gctgctccat cctgaaccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgccagggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttctgg caagggtgcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt atttgatgcg
     1321 gcggaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacgg ccgttgtgtt ttttggcttt
     1501 cggtttcggc agcgggctgg ctccgttcgc gccgggcaca ttcggcactt tggcggcact
     1561 gcctttggcg tttgtgctga ttttgctcgg catagacggg ctactgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccac ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgc ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgcgcctt cttcgcccaa
     2041 acggcgtttc ttgtcggctt cgtattcctg atagttgccg tcgaagaaca cccatttaga
     2101 gtcgccttca cacgccaaga tatgcgtggc gatgcggtcg aggaaccaac ggtcgtgcga
     2161 aatcaccatc acgctgccgg caaattccaa caatgcgtct tccaacgcgc gcagggtttc
     2221 cacgtcaagg tcgttagacg gttcatccag cagcaataca ttgccgccgc tcaacaaggt
     2281 ttttgccaag tgcagacgac cgcgttcgcc gccagacaat tgacctgcaa ttttgctttg
     2341 gtcgctgcct ttgaagttga aacgccccaa atattggcgg gcgggaattt caaactgacc
     2401 aacctgcaaa atgtcgcggc cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgtccgattt tcacctcgcc
     2521 ggaatcaggc tgctctttgc ccgaaatcat tttgaacagc gtagatttac ccgcgccgtt
     2581 cgggccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttatcg ccgaacgatt tggaaacatt tacaaattca atcacttcgt tacccaaacg
     2701 ctcggcaacg ggaataaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgcgttacc gcaacgactg tgccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 cgattccgca tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gcttgctcaa
     3121 caagagtttg cacaaggcaa cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc ggcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacctgccg caataatcgc ttccaagcgg ccctgctctt ctgccaacgc
     3301 gtcaaaatcc gcatcaggat tggcgtactc ggcatacact tcttccaaac gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcgcgc acggtttttt ccggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt gatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacgcccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcagg ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgctta
     3781 tccctttgaa attatataga caaaaaaata ataatgatag gggatcgccg ccccggcaac
     3841 catttcggat tttccaaag
//
LOCUS       DAWGRZ010000175         3841 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000175 DAWGRZ010000000
VERSION     DAWGRZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.175"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(31..1941)
                     /locus_tag="WHT44_001816"
     CDS             complement(31..1941)
                     /locus_tag="WHT44_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ2533957.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGLQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGGKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFHPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     VFEGQGRLKEYIGGYQDYIDAKSREDKIQTASAPKASDAEPAKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINTQLSYPEIFKDYEKAGALQNRAEEIEMLLLEKLERW
                     ELLETKRNGNAV"
     gene            complement(1960..2604)
                     /locus_tag="WHT44_001817"
     CDS             complement(1960..2604)
                     /locus_tag="WHT44_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ2533958.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            complement(2769..3587)
                     /gene="opc"
                     /locus_tag="WHT44_001818"
                     /gene_synonym="opcA"
     CDS             complement(2769..3587)
                     /gene="opc"
                     /locus_tag="WHT44_001818"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ2533959.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHISVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
BASE COUNT          939 a          873 c          965 g         1064 t
ORIGIN      
        1 gtttgaacat ttttttcttc ctgtttgatt tcagacggca ttgccgttcc gtttggtttc
       61 cagcagctcc cagcgttcca gcttttccaa aagcagcatt tcgatttctt cggcgcggtt
      121 ttgcaatgca cctgcttttt cgtaatcttt gaaaatttca ggataggaaa gttgggtatt
      181 gatttcagcc tgctcggctt ccaaagcggc gatttcgtcg ggcagggcat cgagttcgcg
      241 ctgctctttg taggacagtt tgaccgtgcg gttggctttg ggtttttctt tggcgggttc
      301 ggcatcggat gctttgggtg cggatgccgt ctgaatttta tcttcccgcg attttgcgtc
      361 gatatagtcc tgatagccgc cgatgtattc tttcagacgg ccttgtcctt cgaaaacaat
      421 gctttgggta attacgttat caaggaacat acggtcgtgc gagacaagga atactgtgcc
      481 ttgataatcg cgcaacaggt cttcgagcag ctcttgggtg tcgatgtcta agtcgttggt
      541 cggttcgtcc aagaccagga tattggcagg acgggtaaag agttttgcca gcaaaaggcg
      601 gttgcgttct ccgccggaga gcgatgaaac agggctttgc gcacgggctg gatggaacag
      661 gaaatcttcc aaatagctca tgacgtgttt tttcttaccg ccgacttcaa cgtaatcgtt
      721 tccctgtccg agggtgtaaa acacggtgtc gttttcattc aacgcgctgc ggaactggtc
      781 gaaataggcg acttcctgct tactgccgat acggattctg ccgtaggtcg gctgcaattc
      841 gcccaaaatc agcttaagga aggtggtttt gccgatgccg ttggggccga ttaggccgat
      901 tttgtcgccg cgctgcaaga tagcggagaa tttgtccata atgactttgc cgccataggc
      961 aaacgaagcg tgttccaatt cggcgatgat tttgccactt ttctcaccgc tatcgagctt
     1021 gaagttgact tgtccctgta cgttgcggcg ttctgcacgc tggcggcgca gctcttccaa
     1081 acggcgcacg cggccttcgt tgcgggtacg gcgcgcttcg atgcctttgc gtatccatgc
     1141 ttcttcctgt gcgtggaatt tgtcaaagag gcggttgtgt tccgcttcga ctgccaactc
     1201 ttgcgctttt ttctcgctgt atttagagaa cgagccggga taggaacgca aaataccgcg
     1261 atcgagttcg acaatccgcg tggcgatatt gtccaaaaaa cggcggtcgt gggtaatcac
     1321 aaccaagctg ccttcaaacg ctttgagcag attttccagc caaataatcg cgtcgatatc
     1381 caaatggttg gtcggctcgt ccagcagcaa tacgtcgggc ttttgcaccc aagcctgagc
     1441 caaggcgacg cgctttttct gaccgccgga aaggttgccg attttttcat tttccggcaa
     1501 accgagttcc cccaaagtct gcttgactgc cgcatccagt ttccagccgt ccttcgcttc
     1561 gatttcaagt tgcaattcgt tgagttcttt caacaaagcc tcactcgaac cattttccaa
     1621 ctcatggctg acatgatgat aacggcgcaa taaatcacga atttcgccca aaccttcggc
     1681 aacggtatca aatacggttg cgtccttatc aaaaaaggat tcctgcggta cataaacgat
     1741 tttgaggttg ttttgaacaa taatctgccc gtcgtcgagc ttttgcaaac cggcgaggat
     1801 ttttaaaaac gaagacttgc ctgcgccgtt gcgtccgatt aagccgactt tttcgccgct
     1861 gtcgagttga aaagaagttt tgtcgagcaa ggcaacgtgg ccgatggcaa aagaagcgtt
     1921 ttctacagat aatatattca tgatacaaat tctcaacagt taccgtttgg attttaccgc
     1981 aagtttggcg cgggcaattt caaccgcacc cggcaggacg gaaacaataa tgatgccgcc
     2041 catcaccaag cccagattgt tttttacgac ggggaagttg gcaaagaaat agcccgcgta
     2101 agaaaacagg ataacccaca acaagccacc gatgatgttg tagcggataa atttggcata
     2161 gtgcattttc cccataccgg cgacgaaggg ggcgaaggtg cggacgatgg gcataaaacg
     2221 ggcaatgatg atggttttgc cgccgtgttt ttcgtaaaaa cggtgggttt tatcgagata
     2281 ttcacgtcgg aagattttag aatcggggtt ggcgaacagc ctgccgccga aatatttgcc
     2341 gacggtaaaa ttgagcgcgt cgccgagtat ggcggcaagg cttaataatg caaccatcaa
     2401 atgaatatcc ataccgccca gcgcggcaat cccgccggcg gcaaacagca gcgaatcgcc
     2461 gggcagtaag ggcgtaacaa tcaggccggt ttcgcaaaaa acaatcaaaa acagaatcgc
     2521 ataaatccac acaccgtatt gcgccgacag cgcgagcagg tgttggtcga tatggaggat
     2581 gaagtcgatg gcggaggcaa gcacggcgcg ttcctaaaaa aaacaaaccg cgtattttaa
     2641 ccgattggaa aaatgccgtc tgaaaagttt cagacggcat cggctattca aattcatttc
     2701 acgtaaaaac cgcaaaccaa aatagtttgc ggtttggcat ttaaagtgac aatgatgatt
     2761 tcaaatcatc agaattttat gccgacgcgc aagccgtatt cacgaatact ggttttcggg
     2821 atggtgagcg atacgtcgcc actcttggtt gttacactaa actcgccgga ttctttgtaa
     2881 gtgcgttgtt tgtagaacgg ccccgcctcg atgctggcgg attcgcccag ttttttacca
     2941 atatttgcac ccaatccaaa gccccagccg ttggtttttt gattgatgtc ttttttgaga
     3001 ttggtaacgc cggtgtttaa tttatagcgg gaattgaggt caaatttcac ttcagaccaa
     3061 gggttgatat accagccgtt acccagttgg gaaagcaaat ccgcgtgaac tttggctaac
     3121 cacgactgac ggctgctgtg aagcgtatgc ttggtggttt taatgctgtc ttttgaagat
     3181 tcaaaaccca agccggcacc cacacggaaa tttaaagaat cacttaacgt ttgggtgtag
     3241 gtgtagcctg tgtaaagatc gatacggttt tcaggaacgc cggtgggcag ttttacattt
     3301 ttagtcttac ccagcttgtt ctcatctgtt tccaaattaa taatattttt tttgctgcgc
     3361 ccgaaaccgg cttccaagcg gatgccttgg ttggcatcaa aaggaatatc agcacgcacg
     3421 ctgatgtgtt tggcagcttt gtgtttttct ttcaggaaag cacgagttga agaaatggaa
     3481 gagaggtcgg tgtggacggt aaactcatta gcggtttgaa gctcttgtgc agcggcggca
     3541 gtaccggtca gggcaatcat ggcacatgta aaaactgttt ttttcatagt taaaacctct
     3601 aaaatttgga ttgtagtcgg atatggtaac ataacgtaaa taatcgttac gcttacaatt
     3661 atattcttaa gctttcgggg gggggggatt ttacatatat taataaaaat taacaaatag
     3721 ttatttgttt acaacgaatt gttattctca cttggttttc tgttttttat gggaatgacg
     3781 aaattttagt ttgtgtgtat ttatcggaaa aacagaaacc cgccgccgtc attcccgcgc
     3841 a
//
LOCUS       DAWGRZ010000176         3815 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000176 DAWGRZ010000000
VERSION     DAWGRZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.176"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            374..1495
                     /gene="dnaJ"
                     /locus_tag="WHT44_001819"
     CDS             374..1495
                     /gene="dnaJ"
                     /locus_tag="WHT44_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ2533960.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1691..3709
                     /locus_tag="WHT44_001820"
     CDS             1691..3709
                     /locus_tag="WHT44_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ2533961.1"
                     /translation="MNKSLSSSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIVIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGK"
BASE COUNT          807 a          860 c         1141 g         1007 t
ORIGIN      
        1 ggcataggcc ctgcggtgct ggcgaagctg aaggatcagg cttccgtcgg cgcgcccgca
       61 ccaaaaggcc cagccaaacc agtgctgccc gcggataaaa aataaaatag ggggaagtct
      121 gcagccgcat caaatgccgt ctgaacatgc gttcgggcgg cgtttttata acaaaaacac
      181 ttcatggcgg ttggttttat gcctatctaa gtttttgtgt cgtgcatacc tgaagatttc
      241 agacggcatc ggtttatgct gtctgaaaag tgtattccgt ttcagtttgt aagctatggc
      301 agtctgtttg tcttgtgttt tgcgcaattg cccttatttt gagccgtgat tttattttga
      361 attagatgaa aaaatgagta atcaagattt ttatgcgacg ctgggtgtgg caagaacagc
      421 taccgatgat gagattaaaa aagcctaccg gaaattggcg atgaaatacc atcccgaccg
      481 caatcctgac aataaagagg cggaagagaa gtttaaagaa gtacaaaagg cgtatgaaac
      541 tttgtccgac aaggaaaagc gcgctatgta cgaccagtat ggtcatgcgg cgtttgaagg
      601 cggcggacag gggggcttcg gagggtttgg cggatttggc ggtgcgcagg gttttgactt
      661 tggggatatt ttcagccaaa tgtttggagg cggttcgggg cgcgcccagc ctgattatca
      721 gggtgaggac gttcaagtcg gtatcgaaat cacgcttgaa gaagccgcaa aaggtgtgaa
      781 gaaacgcatc aatattccga cttatgaagc gtgtgatgtc tgtaacggca gtggcgcgaa
      841 accggggaca tccccggaaa cctgcccgac ttgcaaaggt tcgggtacgg tgcacatcca
      901 gcaggcgatt ttccgtatgc agcagacttg tccgacctgc cacggtgcgg gcaaacacat
      961 taaagaacct tgcgtcaaat gccgtggcgc ggggcggaat aaggcggtca agacggtgga
     1021 agtcaatatt cccgccggta tcgatgacgg gcagcgtatc cgtttgagcg gcgaaggcgg
     1081 gccgggtatg cacggtgcgc ctgccggcga cttgtatgta accgtccgca ttcgggcgca
     1141 taagattttc caacgcgacg gtctggactt gcattgcgaa ctgccgatca gttttgccac
     1201 ggctgctttg ggcggggagt tggaagtgcc gaccttggac ggaaaggtca agctcaccgt
     1261 ccccaaagaa acccaaaccg gcaggaggat gcgcgtgaag ggtaagggtg tcaaatcttt
     1321 acgcagcagc gcgaccggcg atttgtactg ccatattgtt gtcgaaacgc ctgtcaattt
     1381 gaccgaccgt caaaaagagc ttttggaaga atttgagcgg atttctaccg gcttggaaaa
     1441 ccaaacaccg cgcaagaaat cgtttttaga caagctgcgc gatttgtttg attgatttta
     1501 aggttcggaa acaagcagcc gtatcgggga atctccttga tacggctgtt tttatttgtt
     1561 taaaaatagt ttttattttc aatggggtat gaggcagggt gggataactg tttttaactg
     1621 ttctttttaa aacttgacat catggcgtga tgccaacaat atgtgaacgt ctgttgtcaa
     1681 aggaagaata atgaataaat ctttatccag ttcggtagaa gaataccgcg agctgacgct
     1741 ccgaggcatg atactcggtg cattgatcac tgtaattttt actgcgtcca atgtttacct
     1801 cggtttgaaa gtcgggctga cctttgcctc gtcgattccg gcggcggtga tttcgatggc
     1861 ggttttaaag tttttcaaag gcagcaatat tttggaaaac aacatggtgc agacccaagc
     1921 ctcggctgcg ggtacgcttt cgaccatcat cttcgtcctg cccggtttgc tgatggcggg
     1981 ctactggagc ggtttcccgt tctggcagac gacgctttta tgtattgccg gcgggatttt
     2041 gggggtgatt ttcaccattc ctctgcgtta cgcaatggtg gtgaaaagcg atttgcctta
     2101 tccggaaggt gtggcggctg ctgaaatttt gaaagtgggc ggtcatgaag aaggggataa
     2161 ccgtcagggc ggcagcggca tcaaagagct ggcggccggc ggtgcgttgg cgggattgat
     2221 gagcttttgc gccggaggtc tgcgcgtgat tgccgacagc gcgagttatt ggtttaaaag
     2281 cggtacggcg attttccagc tgccgatggg cttttcactg gcattgttgg gcgcgggcta
     2341 tttggtcgga ctgacgggcg gtatcgccat cctgttgggc atttcgattg cttggggcat
     2401 tgccgtgccg tatttctcct cacacattcc gcaaccttcc gatatggaaa tggcggcgtt
     2461 tgcgatgaag ctgtggaagg agaaagtgcg ttttatcggt gcggggacta ttggcattgc
     2521 ggcggtttgg acgctgttga tgctgctcaa gccgatggtg gaaggcatga agatgtcgtt
     2581 caagagtttt ggcggcggtg cgcccgctgc ggaacgcgcc gaacaggatt tgtcgcctaa
     2641 ggctatgatt ttttgggtgc tggcgatgat gtttgtttta ggcgtgtcgt tttaccactt
     2701 tatcggcgat tcgcacatta cgggcggcat ggcttggctt ttggtggtcg tttgcacgct
     2761 tttggcttcc gtcatcggct ttttggtcgc cgccgcctgc ggttatatgg caggtttggt
     2821 cggctcgtct tccagcccga tttccggcgt gggcatcgtg tccgtcgtcg ttatttcact
     2881 ggttttgctg ctggtaggcg aatccggagg tttgttggcg gatgaggcta accgcaaatt
     2941 tttgctggca ctgactttgt tttgcggctc ggcagtaatc tgcgtggctt cgatttccaa
     3001 tgacaacctg caagacttga aaaccggcta cctgctcaaa gccacgcctt ggcggcagca
     3061 agtcgccctg attatcggct gtatcgttgg tgcgctggtt atttcgcccg tgttggaact
     3121 gctttacgaa gcctacggct ttaccggcgc aatgccgcgc gaaggcatgg acgcggcgca
     3181 ggctttggca gcccctcaag cgactttgat gacgaccatc gcgtcgggca ttttcgccca
     3241 caaccttgaa tgggtctata tctttaccgg tatcgtgatt ggagcagtat taatcgtcgt
     3301 cgatttggtg ttgaaaaaat catcaggcgg caaacttgcc ctgcccgtcc ttgcggtcgg
     3361 tatgggtatt tatctgccgc cgtccgtcaa tatgcccatc gtggcaggcg cggtgttggc
     3421 ggcggtgttg aaacacatca tcggtaaaaa agcggaaaac cgcgaaggcc gtctgaaaaa
     3481 cgccgagcgc atcggaacct tgttctccgc cggcctgatt gtcggtgaaa gcctgatcgg
     3541 tgtgattatg gcgtttatta ttgccttctc cgtgaccaac ggcggctcgg atgcgccgct
     3601 cgcgttgaat ctgcaaaact gggatgccgc cgcttcttgg ctgggtttgg cgttcttcgt
     3661 taccgggatg tttttctttg cacagcgcgt actgaaggcg ggcaagtagg ctgtcggaaa
     3721 aaatgccgtc tgaaacgttc agacggcatt ttttatcggt aaagcggaag gcggagcttt
     3781 tcggcttgcg cccacgtttt gccggcaagg tcttt
//
LOCUS       DAWGRZ010000177         3752 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000177 DAWGRZ010000000
VERSION     DAWGRZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.177"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            330..3581
                     /gene="nalP"
                     /locus_tag="WHT44_001821"
     CDS             330..3581
                     /gene="nalP"
                     /locus_tag="WHT44_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HEZ2533962.1"
                     /translation="MRTTPTFPTKTFKPTAMALAVATTLSACLGGGGGGTSAPDFNAG
                     GTGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPPNL
                     HTGDFPNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGY
                     NENYKNYTAYMRKEAPEDGGGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDLVSHI
                     IGGRSVDGRPAGGIAPDATLHIMNTNDGTKNEMMVAAIRNAWVKLGERGVRIVNNSFG
                     TTSRAGTADLFQIANSEEQYRQALLDYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNML
                     FIFSTGNDAQAQPNTYALLPFYEKDAQKGIITVAGVDRSGEKFKREMYGEPGTEPLEY
                     GSNHCGITAMWCLSAPYEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSND
                     NLRTTLLTTAQDIGAVGVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSF
                     RNDISGTGGLIKKGGSQLQLHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYN
                     GAASGGSLNSDGIVYLADTDQSGANETVHIKGSLQLDGKGTLYTRLGKLLKVDGTAII
                     GGKLYMSARGKGAGYLNSTGRRVPFLSAAKIGQDYSFFTNIETDGGLLASLDSVEKTA
                     GSEGDTLSYYVRRGNAARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATP
                     ETVETAAADRTDMPGIRPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHAD
                     MQGRRLKAVSDGLDHNGTGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGEN
                     TTAAATLGMGRSTWSENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRS
                     TGADEHAEGSVNGTLMQLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALG
                     WSGNSLTEGTLVGLAGLKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATG
                     KTGARNMPHTRLVAGLGADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1016 a         1019 c         1003 g          714 t
ORIGIN      
        1 taaagaatcg ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttcatcca
       61 ctataactag ggaaattcaa attaagttag aattatccct atgagaaaaa gccgtctaag
      121 ccggtataaa caaaataaac tcattgagct atttgtcgaa agttcaaatt tccattttaa
      181 aacaattagt aaaatcgagt ttatcctagt tgtccaagac aacccctata ataatataat
      241 tcaaaatata aaaatgggtt acatctaaac attacggaat ttttattccc tcgcctgaat
      301 tctattgtca gattcaagga gacctcatca tgcgaacgac cccaaccttc cctacaaaaa
      361 ctttcaaacc gactgccatg gcgttagctg ttgcaacaac actttctgcc tgcttaggcg
      421 gcggcggagg cggcacttct gcgcccgact tcaatgcagg cggtaccggt atcggcagca
      481 acagcagagc aacaacagcg aaatcagcag cagtatctta cgccggtatc aagaacgaaa
      541 tgtgcaaaga cagaagcatg ctctgtgccg gtcgggatga cgttgcggtt acagacaggg
      601 atgccaaaat caatgccccc ccccccccga atctgcatac cggagacttt ccaaacccaa
      661 atgacgcata caagaatttg atcaacctca aacctgcaat tgaagcaggc tatacaggac
      721 gcggggtaga ggtaggtatc gtcgacacag gcgaatccgt cggcagcata tcctttcccg
      781 aactgtatgg cagaaaagaa cacggctata acgaaaatta caaaaactat acggcgtata
      841 tgcggaagga agcgcctgaa gacggaggcg gtaaagacat tgaagcttct ttcgacgatg
      901 aggccgttat agagactgaa gcaaagccga cggatatccg ccacgtaaaa gaaatcggac
      961 acatcgattt ggtctcccat attattggcg ggcgttccgt ggacggcaga cctgcaggcg
     1021 gtattgcgcc cgatgcgacg ctacacataa tgaatacgaa tgatggaacc aagaacgaaa
     1081 tgatggttgc agccatccgc aatgcatggg tcaagctggg cgaacgtggc gtgcgcatcg
     1141 tcaataacag ttttggaaca acatcgaggg caggcactgc cgaccttttc caaatagcca
     1201 attcggagga gcagtaccgc caagcgttgc tcgactattc cggcggtgat aaaacagacg
     1261 agggtatccg cctgatgcaa cagagcgatt acggcaacct gtcctaccac atccgtaata
     1321 aaaacatgct tttcatcttt tcgacaggca atgacgcaca agctcagccc aacacatatg
     1381 ccctattgcc attttatgaa aaagacgctc aaaaaggcat tatcacagtc gcaggcgtag
     1441 accgcagtgg agaaaagttc aaacgggaaa tgtatggaga accgggtaca gaaccgcttg
     1501 agtatggctc caaccattgc ggaattactg ccatgtggtg cctgtcggca ccctatgaag
     1561 caagcgtccg tttcacccgt acaaacccga ttcaaattgc cggaacatcc ttttccgcac
     1621 ccatcgtaac cggcacggcg gctctgctgc tgcagaaata cccgtggatg agcaacgaca
     1681 acctgcgtac cacgttgctg acgacggctc aggacatcgg tgcagtcggc gtggacagca
     1741 agttcggctg gggactgctg gatgcgggta aggccatgaa cggacccgcg tcctttccgt
     1801 tcggcgactt taccgccgat acgaaaggta catccgatat tgcctactcc ttccgtaacg
     1861 acatttcagg cacgggcggc ctgatcaaaa aaggcggcag ccaactgcaa ctgcacggca
     1921 acaacaccta tacgggcaaa accattatcg aaggcggttc gctggtgttg tacggcaaca
     1981 acaaatcgga tatgcgcgtc gaaaccaaag gtgcgctgat ttataacggg gcggcatccg
     2041 gcggcagcct gaacagcgac ggcattgtct atctggcaga taccgaccaa tccggcgcaa
     2101 acgaaaccgt acacatcaaa ggcagtctgc agctggacgg caaaggtacg ctgtacacac
     2161 gtttgggcaa actgctgaaa gtggacggta cggcgattat cggcggcaag ctgtacatgt
     2221 cggcacgcgg caagggggca ggctatctca acagtaccgg acgacgtgtt cccttcctga
     2281 gtgccgccaa aatcgggcag gattattctt tcttcacaaa catcgaaacc gacggcggcc
     2341 tgctggcttc cctcgacagc gtcgaaaaaa cagcgggcag tgaaggcgac acgctgtcct
     2401 attatgtccg tcgcggcaat gcggcacgga ctgcttcggc agcggcacat tccgcgcccg
     2461 ccggtctgaa acacgccgta gaacagggcg gcagcaatct ggaaaacctg atggtcgaac
     2521 tggatgcctc cgaatcatcc gcaacacccg agacggttga aactgcggca gccgaccgca
     2581 cagatatgcc gggcatccgc ccctacggcg caactttccg cgcagcggca gccgtacagc
     2641 atgcgaatgc cgccgacggt gtacgcatct tcaacagtct cgccgctacc gtctatgccg
     2701 acagtaccgc cgcccatgcc gatatgcagg gacgccgcct gaaagccgta tcggacgggt
     2761 tggaccacaa cggcacgggt ctgcgcgtca tcgcgcaaac ccaacaggac ggtggaacgt
     2821 gggaacaggg cggtgttgaa ggcaaaatgc gcggcagtac ccaaaccgtc ggcattgccg
     2881 cgaaaaccgg cgaaaatacg acagcagccg ccacactggg catgggacgc agcacatgga
     2941 gcgaaaacag tgcaaatgca aaaaccgaca gcattagtct gtttgcaggc atacggcacg
     3001 atgcgggcga tatcggctat ctcaaaggcc tgttctccta cggacgctac aaaaacagca
     3061 tcagccgcag caccggtgcg gacgaacatg cggaaggcag cgtcaacggc acgctgatgc
     3121 agctgggcgc actgggcggt gtcaacgttc cgtttgccgc aacgggagat ttgacggtcg
     3181 aaggcggtct gcgctacgac ctgctcaaac aggatgcatt cgccgaaaaa ggcagtgctt
     3241 tgggctggag cggcaacagc ctcactgaag gcacgctggt cggactcgcg ggtctgaagc
     3301 tgtcgcaacc cttgagcgat aaagccgtcc tgtttgcaac ggcgggcgtg gaacgcgacc
     3361 tgaacggacg cgactacacg gtaacgggcg gctttaccgg cgcgactgca gcaaccggca
     3421 agacgggggc acgcaatatg ccgcacaccc gtctggttgc cggcctgggc gcggatgtcg
     3481 aattcggcaa cggctggaac ggcttggcac gttacagcta cgccggttcc aaacagtacg
     3541 gcaaccacag cggacgagtc ggcgtaggct accggttctg acggacagga agcagacagc
     3601 cgcaaagatc acggtctttg cggctgtttc ttatgaaaag aaaaccctat tccaattgcc
     3661 tgcttctatt gtttcaagac ttcttccaaa gattcggcat taatcagatg tatagcggat
     3721 taacaaaaat caggacaagg cggcgaagcc gc
//
LOCUS       DAWGRZ010000178         3708 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000178 DAWGRZ010000000
VERSION     DAWGRZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.178"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(153..398)
                     /locus_tag="WHT44_001822"
     CDS             complement(153..398)
                     /locus_tag="WHT44_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533963.1"
                     /translation="MLKSIELNSHIRNRLAEYLKGRGMDFQTAMQEEKGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            510..938
                     /locus_tag="WHT44_001823"
     CDS             510..938
                     /locus_tag="WHT44_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ2533964.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            950..1426
                     /locus_tag="WHT44_001824"
     CDS             950..1426
                     /locus_tag="WHT44_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ2533965.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GKQAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(1776..2264)
                     /locus_tag="WHT44_001825"
     CDS             complement(1776..2264)
                     /locus_tag="WHT44_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ2533966.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(2332..3387)
                     /gene="aroG"
                     /locus_tag="WHT44_001826"
     CDS             complement(2332..3387)
                     /gene="aroG"
                     /locus_tag="WHT44_001826"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ2533967.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAEHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAS"
BASE COUNT          837 a          972 c          872 g         1027 t
ORIGIN      
        1 ccgcctgcgc gggaatgacg gctcaaaagt tacgaaacga aaaataaccg aaaccaaaca
       61 agtcggattc ccgcgcgggc gggaatgacg ataagcgtaa tttcaatctg ccatattgtc
      121 ttttcacttg cctgaaaaca gatttcagcc acctatccct gcatccggcg gctgatgtag
      181 tcggcaatca aatcccgctt ttcccaaaaa aacttctgca ttttttgttc ggaatacagt
      241 ttgcggacca gagtgggcaa accgctgtgg acgatggcgg cgatttcttt gttgcctttt
      301 tcttcctgca ttgccgtctg aaaatccata cccctgcctt tcagatattc tgcaaggcgg
      361 ttgcggatgt gggaattgag ttcgatggat ttgagcattt tgcctccgtt ttgcggtaag
      421 gatttattcg atttgtttat ccgcctgatt ttatcacgtt tcccaagcgg tttgcctgta
      481 caataccgct tttcggcagg gtgtgggcga tgatgttttc ttggttcaag ctgtttcact
      541 tgttttttgt catttcgtgg tttgcagggc tgttttacct gccgaggatt ttcgtcaata
      601 tggcgatgat tgatgtgccg cgcggcaatc ccgagtatgt gcgtctgtcg ggcatggcgg
      661 tgcggctgta ccgttttatg tcgccgttgg gcttcggcgc ggtcgtgttc ggcgcggcga
      721 taccgtttgc cgccggctgg tggggcagcg gctgggtaca cgtcaaactg tgtttgggct
      781 tgatgctctt ggcttaccag ttgtattgcg gcgtgctgct gcgccgtttt caggattaca
      841 gcaatgcttt ttcacaccgc tggtaccgcg tgttcaacga aatccccgtg ctgctgatgg
      901 ttgccgcgct gtatctggtc gtgttcaaac cgttttgacg gagcatctta tgccgataga
      961 aatcgaacgc cgttttttga ttggaaacga caactggcgg caatacgccg acgaaccgct
     1021 gctgttgagg cagggttatc tgtctgttga aaaagaacgc accgtccgtg tccgcattgc
     1081 cggaaaacag gcgtggctga cgctgaaagg ctatatttcg gaaatcagcc gcagtgagtt
     1141 tgaatacgaa atcccgcttg ccgatgcgga aaagatgatg gaaacgatgt gcccgtttaa
     1201 aatggaaaaa tggcgttatc cggtcagatg gggcggcagc ctgtttgaaa tcgatgtttt
     1261 tcttggcgat aatgcgcctt tggtcgtcgc ggaaatcgag ttgcccgacg agaatgccga
     1321 ttttgaccgt ccggactggc tgggacggga aatcactgcc gacggtatgt ttaccaatgc
     1381 ttatttgagc aggcatccgt tctcaagctg gaaaaatgcc gtctgaaacg gcttcagacg
     1441 gcattctccg acaaccatcg caaatccaaa gacagcctct atatccttat ttctgctttc
     1501 tttgttccat tattcccaat aaatccccat aacctaaaat ctcgtcattc ccgcgcaggc
     1561 gggaatccgg ttctttaaac ttcggttttt tccgataaat tgctttagct tttcgtttcc
     1621 ggattcccgc ttttgcggga atgacgatgg aagattgttg ttatttcgga taaattaccc
     1681 taactttaaa atcccggatt cccatcttcg ggggaataac aatattttct atttcagccc
     1741 atcatttgcg ggggatttta tagagagttt atatttcaat atctcaaata atgcacaaaa
     1801 gcatagcgcg tgcctttgct gctgacacgg cgttccgtcc gctctgcttc tttccaatgc
     1861 gtccggtcta ttgcggggaa aaatgcatct ccttccacag acaaatccac ttcggttatc
     1921 cgcaaatcgg tcgccaatgg catcgcttgt ccgtatatct gcgcgccgcc cataatgacg
     1981 gcttcttccg cgcctgcgca caatgccaat gccgcctcca aacttgccgc cgtttccgcg
     2041 cctgccgcgc aataatccgc ctgccggctg atgacgatgt tcctccgtcc gggcaggggt
     2101 ttgacgggca gggattccca cgttttccgc cccataatga cgggtttgcc caaggtatag
     2161 gctttgaaaa atgcgaaatc ttcggggatg tgccaaggca tagcattgcc cgccccgatg
     2221 cacaggtttt ccgcacacgc cgcaattagg gttattttga gcatatttcc tccacaaaaa
     2281 tgccgtctga acgcttcaga cgacatttta tgacattctg cgtcaaaaat ctcaactggc
     2341 gcgcgccata cgttttttgt ttgcacctgc caacaatgcc aacagttctt cagtcgcgcc
     2401 ccaaccgata cacgcatcgg taatgctctt gccgtacact tccggcttgt cctgtctgcc
     2461 ttcgaccaaa tggctttcca ccatcacgcc catgatattg ccgccgtcct gttccaattg
     2521 ggcggcaatg tcttgtgcca cttccatctg ccgagtgtaa tccttgcggc tgttggcgtg
     2581 gctgcaatct atcatcagct tgtcggttac ccctgccgca cgcagttgtt ccgccgcctc
     2641 gctgacgtgt tccgcatcat aattcggctc tttgccgccg cgcaaaatga catgacagtc
     2701 gggattgccg ccggtatgga caatggcgga atgcccggcc ttggttacag acaggaaatg
     2761 atgcgaatgg ctcgccgcac cgattgcgtc gatggcaatc ttcaaattgc cgtccgtacc
     2821 gtttttaaag ccgacggggc aggacagccc gcttgccaat tcgcggtgaa cttggctttc
     2881 ggtcgtccgc gcaccgattg ccccccaaga aatcaagtcc gcataatatt gcggcgtaat
     2941 catatccaaa aactcggtag aggcaggcat acccatattg ttcagcgaca acaacaggct
     3001 gcgcgcctga cgcaaaccga aattgatgtc aaacgtaccg tccaaatgcg ggtcgttaat
     3061 caaacctttc caacccaccg tcgtcctcgg cttctcgaaa taaacgcgca tcacaatcaa
     3121 aagctcgttt tcatactgct tgcggagttt caacaaacgc tccgcatatt ccaacgccgc
     3181 tttcggatcg tgaatcgaac acggcccgat aataaccaac agccgcttgt ccctgccgtg
     3241 aaccaaatcg gaaatttcct gacgggtgcg gtgaaccaag cccgaagcct ctttggaaat
     3301 cggcagctcg taaagatggg ctatcggcgg caacaactct ttaacttctt taatcttaat
     3361 atcgtctgtg gggtaatggt gtgtcatctt gcatctttcc tgatgggttt ttatacgcgg
     3421 cttaggttac atcgtttacc ttgcacaaac aagtgcaaca tattactaca aagctatttt
     3481 atttcaaaat aactgatata tcttttttta tagattttaa atttcatatt tttattattt
     3541 tagaaatttt atttcaaata aaaagaaaca gattgataaa aacaataccc gccattcatt
     3601 tggacaaacc tgcacggaac atccgttttc ccatttccac gacaatccgg tacagaacac
     3661 ccccgctgcc gattccgcct tcacatccgg caaaatttca tacacaag
//
LOCUS       DAWGRZ010000179         3694 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000179 DAWGRZ010000000
VERSION     DAWGRZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.179"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            60..689
                     /locus_tag="WHT44_001827"
     CDS             60..689
                     /locus_tag="WHT44_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ2533968.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLADLGEEPYKFDKELPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            864..3242
                     /locus_tag="WHT44_001828"
     CDS             864..3242
                     /locus_tag="WHT44_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HEZ2533969.1"
                     /translation="MKPLQMLPIAALLGSIFGNPVFAADEAATETTPVKAEVKAVRVK
                     GQRNAPAAVERVNLNRIKQEMIRDNKDLVRYSTDVGLSDSGRHQKGFAIRGVEGDRVG
                     VSIDGVNLPDSEENSLYARYGNFNSSRLSIDPELVRNIDIVKGADSFNTGSGALGGGV
                     NYQTLQGRDLLLPERQFGVMMKNGYSTRNREWTNTLGFGVSNDRVDAALLYSQRRGHE
                     TESAGKRGYPVEGAGSGANIRGSARGIPDPSQHKYHSFLGKIAYQINDNHRIGASLNG
                     QQGHNYTVEESYNLLASYWREADDVNRRRNTNLFYEWTPESDRLSMVKADVDYQKTKV
                     SAVNYKGSFPTNYTTWETEYHKKEVGEIYNRSMDTTFKRITLRMDSHPLQLGGGGRHR
                     LSFKTFAGQRDFENLNRDDYYFSGRVVRTTNSIQHPVKTTNYGFSLSDQIQWNDVFSS
                     RAGIRYDHTKMTPQELNADCHACDKTPPAANTYKGWSGFVGLAAQLSQTWRLGYDVTS
                     GFRVPNASEVYFTYNHGSGTWKPNPNLKAERSTTHTLSLQGRGDKGTLDANLYQSNYR
                     NFLSEEQNLTVSGTPGCTEEDAYYYRCSDPYKEKLDWQMKNIDKARIRGIELTGRLNV
                     DKVASFVPEGWKLFGSLGYAKSKLSGDNSLLSTQPLKVIAGIDYESPSEKWGVFSRLT
                     YLGAKKVKDAQYTVYENKGWGTPLQKKVKDYPWLNKSAYVFDMYGFYKPAKNLTLRAG
                     VYNLFNRKYTTWDSLRGLYSYSTTNAVDRDGKGLDRYRAPGRNYAVSLEWKF"
     gene            complement(3450..3572)
                     /locus_tag="WHT44_001829"
                     /pseudo
     CDS             complement(3450..3572)
                     /locus_tag="WHT44_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
BASE COUNT          995 a          939 c          946 g          814 t
ORIGIN      
        1 gggcaagtgc ggattttgcc cgccccgcat ccttatccct taccgacaaa ggaaaactta
       61 tgagtgaaac cgaaaatcaa gccctgacgt ttgccaaacg tctgaaggcg gataccacgg
      121 cggttcacga cagcgtggat aacctcgtta tgtctgtcca accgtttgtc agcaaagaaa
      181 actacatcaa atttttgaaa ctccaatccg ttttccacaa ggccgtcgac cacatctata
      241 aagatgccga attaaacaaa gccattcccg agctggaata catggcgcga tacgatgccg
      301 taacgcaaga ccttgcagat ttgggcgaag aaccttacaa attcgacaaa gaattaccgc
      361 atgaaaccgg caacaaagca atcggctggc tttattgcgc cgaaggatcc aatttgggcg
      421 cggcgttttt gttcaaacac gcgcaaaaac tcgattacaa cggcgaacac ggtgcgcgcc
      481 acctcgcacc gcatcccgac gggcgcggca aacactggcg cgccttcgtc gagcatctga
      541 acgctttgaa cctgactccc gaagccgaag cggaagccat ccaaggcgcg cgcgaagcct
      601 ttgcattcta caaagtcgtg ttgcgcgaaa ccttcggctt ggcagccgat gccgaagcac
      661 cggaaggaat gatgccgcac aggcactaaa aaataatcga accaaataaa caaggtctcg
      721 gcatagctgt ttgcagagac ctttaattac acggcgcggc tttgtttgca tggattactg
      781 tcttattaaa tattaatgat tatcataaaa tctattattc gctaaccgat ggatgaacaa
      841 tccatacatc ttgagttgat aatatgaaac cattacaaat gctccctatc gccgcgctgc
      901 tcggcagtat tttcggcaat ccggtctttg cggcagatga agctgcaact gaaaccacac
      961 ccgttaaggc agaggtaaaa gcagtgcgcg ttaaaggtca gcgcaatgcg cctgcggctg
     1021 tggaacgcgt caaccttaac cgtatcaaac aagaaatgat acgcgacaat aaagacttgg
     1081 tgcgctattc caccgatgtc ggcttgagcg acagcggccg tcatcaaaaa ggctttgcca
     1141 ttcgcggcgt ggaaggcgac cgtgtcggcg ttagtattga cggcgtaaac ctgcctgatt
     1201 ccgaagaaaa ctcgctgtac gcccgttatg gcaacttcaa cagctcgcgt ctgtctatcg
     1261 accccgaact cgtgcgcaac atcgacatcg taaaaggggc ggactctttc aataccggca
     1321 gcggcgcctt gggcggcggt gtgaattacc aaaccctgca aggacgtgac ttactgttgc
     1381 ctgaacggca gttcggcgtg atgatgaaaa acggttacag cacgcgtaac cgtgaatgga
     1441 caaataccct cggtttcggc gtgagcaacg accgcgtgga tgccgctttg ctgtattcgc
     1501 aacggcgcgg ccatgaaact gaaagcgcgg gcaagcgtgg ttatccggta gagggtgctg
     1561 gtagcggagc gaatatccgt ggttctgcgc gcggtattcc tgatccgtcc caacacaaat
     1621 accacagctt cttgggtaag attgcttatc aaatcaacga caaccaccgc atcggcgcat
     1681 cgctcaacgg tcagcagggg cataattaca cggttgaaga gtcttacaac ctgcttgctt
     1741 cttattggcg tgaagctgac gatgtcaaca gacggcgtaa caccaacctc ttttacgaat
     1801 ggacgccgga atccgaccgg ttgtctatgg taaaagcgga tgtcgattat caaaaaacca
     1861 aagtatctgc ggtcaactac aaaggttcgt tcccgacgaa ttacaccaca tgggaaaccg
     1921 agtaccataa aaaggaagtt ggcgaaatct ataaccgcag catggataca accttcaaac
     1981 gtattacgct gcgtatggac agccatccgt tgcaactcgg gggggggggg cgacaccgcc
     2041 tgtcgttcaa aacctttgcc gggcagcgtg attttgaaaa cttaaaccgc gacgattact
     2101 acttcagcgg ccgtgttgtt cgaaccacca acagtatcca gcatccggtg aaaaccacca
     2161 actacggttt ctcgctgtcc gaccaaatcc aatggaacga cgtgttcagt agccgcgcag
     2221 gtatccgtta cgaccacacc aaaatgacgc ctcaggaatt gaatgccgac tgtcatgctt
     2281 gtgacaaaac accgcctgca gccaacactt ataaaggctg gagcggattt gtcggcttgg
     2341 cggcgcagct gagccaaaca tggcgtttgg gttacgatgt gacctcaggt ttccgcgtgc
     2401 cgaatgcgtc tgaagtgtat ttcacttaca accacggttc gggcacttgg aagcctaatc
     2461 ctaatttgaa ggcagaacgc agcaccaccc acaccctgtc cttgcagggg cgcggcgaca
     2521 aagggacact ggatgccaac ctgtatcaaa gcaattaccg aaacttcctg tcggaagagc
     2581 agaatctgac tgtcagcggc acacccggct gtactgagga ggatgcttac tactatagat
     2641 gcagcgaccc ctacaaagaa aaactggatt ggcagatgaa aaatatcgac aaggccagaa
     2701 tccgcggtat cgagctgaca ggccgtctga atgtggacaa agtagcgtct tttgttcctg
     2761 agggttggaa actgttcggc tcgctgggtt atgcgaaaag caaactgtcg ggcgacaaca
     2821 gcctgctgtc cacacagccg ctgaaagtga ttgccggtat cgactatgaa agtccgagcg
     2881 aaaaatgggg cgtattctcc cgcctgacct atctaggcgc gaaaaaggtc aaagacgcgc
     2941 aatacaccgt ttatgaaaac aagggctggg gtacgccttt gcagaaaaag gtaaaagatt
     3001 acccgtggct gaacaagtcg gcttatgtgt ttgatatgta cggcttctac aaaccggcta
     3061 aaaacctgac tttgcgtgca ggcgtgtaca acctgttcaa ccgcaaatac accacttggg
     3121 attccctgcg cggtttatat agctacagca ccaccaatgc ggtcgaccgc gatggcaaag
     3181 gcttagaccg ctaccgcgcc ccaggccgca attacgccgt atcgctggaa tggaagtttt
     3241 aatctggtat tattgaatta atcgccttgt tgaaaattaa agccgtccga attgtgttca
     3301 agaattcatt cggacggctt tttaccgtaa atctgtgtga tggggtttta tattgataca
     3361 gtatccgaac tcaggcaggg ggtaatgttg caggattaaa aatgccgtct gaaaaatgtt
     3421 tcagacggca ttttttggtt gccgatatgt taacggacga cgttggcgcg catgaccacg
     3481 cggacgtgga acgagcctgc ctgacagtcg tattgtccgc cttgcagcgg ctgcttgtcg
     3541 aagtagctgt ggaagccggt aacgccgcgt ccgcaagggt ggcggcagat gccgcattgt
     3601 gtttataatg ccgtgtcaga cgtttgattt atgggaaaat aatgatgctt accaatcagg
     3661 aagtaaccgg tgtcaaactg ggataccgca aagc
//
LOCUS       DAWGRZ010000180         3555 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000180 DAWGRZ010000000
VERSION     DAWGRZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.180"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            61..332
                     /locus_tag="WHT44_001830"
                     /pseudo
     CDS             61..332
                     /locus_tag="WHT44_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            372..1406
                     /locus_tag="WHT44_001831"
     CDS             372..1406
                     /locus_tag="WHT44_001831"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ2533970.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            1612..2193
                     /locus_tag="WHT44_001832"
     CDS             1612..2193
                     /locus_tag="WHT44_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ2533971.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIAQHHL
                     PEFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            2262..2444
                     /locus_tag="WHT44_001833"
     CDS             2262..2444
                     /locus_tag="WHT44_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ2533972.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            2441..3202
                     /gene="kdsB"
                     /locus_tag="WHT44_001834"
     CDS             2441..3202
                     /gene="kdsB"
                     /locus_tag="WHT44_001834"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ2533973.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTACQAHGIEVVMTSNRHESGTTRLAEASVALKLPPHLIVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAIRAGKREMPSETAVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            3225..>3555
                     /locus_tag="WHT44_001835"
     CDS             3225..>3555
                     /locus_tag="WHT44_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ2533974.1"
                     /translation="MQQHIEKWQHLSREEQKILAEVWGLVQNDDQEVHYEMLKLNAPD
                     EASGEFWFRMAETLSTLPPNRSLGLRMNGGRLATAVSILSVMIEDNPDIPQLWAQKIT
                     ALNYSGLN"
BASE COUNT          885 a          882 c          987 g          801 t
ORIGIN      
        1 aaggcgaggc aacgccgtac tggtttttgt taatccacta taaatgaaag atactgaaaa
       61 atgaaagaga tgaaacctgt ccgttatcat attggcgata tgcccgagac ttcaaaacaa
      121 accgcctccc gtcatgacga cagggcagtg ggtgttgacg atgattgttt tcatgattcc
      181 tttggtcaat ttttgtttgg gtgttcggca gaggcaaccc gaaccgcgcc aatttctgta
      241 aagcgcagtt gcttatttac ctgattggtt cgcttatcgg tttggtcttc gcgttgttta
      301 taggtgggtc tgtatcaggt acgcatgatt aatgccccgg gctgattttg cttcgaggat
      361 ttgtatcgaa tatgccgaat tgtttcaaat ttcataccgt tatcgaacgg cattggcaaa
      421 aaccttatcc ggttttgtct tttctgctta agccgctctc cgggctgttt gccaaaattg
      481 cggcaaaacg gcggacggat tttttatcgg gaaaacggca aagcgaaaag ctgcccgtgc
      541 ctgtggtcgt ggtcggcaat attcacgcgg gtgggacggg gaaaacgccg attgttgccg
      601 cgctggtgtc gggtttgcag gaaaagggcg tcaaggtcgg catcatcagc cggggctacg
      661 ggcgcaagag caaggcggtt catgtattga atgctgagag ccgagcggaa gatgcgggcg
      721 atgagccttt gctgctgttc cgcaaaaccg gtgcgccgac ggcggtgggc agcagccgtg
      781 cagaggcagg cagggcgttg ctggcggcgc atcccgacat cggactgatt gtggcggacg
      841 acggtttgca gcattacgcc ctgcggcgag atgtggaaat cgcggtgttt ccggcggcgg
      901 atacggggcg cacggatttg gatttactgc ccaacggcag tttgcgcgaa cctttgttgc
      961 ggctggattc ggtggatgcg gtcgtcgtca gcggcggcaa ggcggatgcg ctgtttaggc
     1021 cgtctgaaaa tatgtttcac agccgtatcg aagcgggacg gatttaccgt ttgaacaatc
     1081 cgtccgaaat actggacaca ggccgtctga aaaatcaaac cgtcgtcgcc gtggcaggta
     1141 ttgccaagcc ggcgcggttt tttgattcgt tgcggaatat gggcattacc gtgaagcgaa
     1201 ccgtcgcgct gcccgaccac gccgacattt cggcggcaga tttgcccgat gcggacgcgg
     1261 tcattattac ggagaaagat gcggtcaaat tttcagacgg catttgcacc gataatgttt
     1321 gggtgttgcc cgtttgtgcg ataatcgaac ctgatttggc ggcgtttgtg ttggagcggt
     1381 tggaagatgt accgaaggcc gtctgaaagc acggtttggg cggagtgatt acggatttga
     1441 ataagaacgc ctcgcgccat cattcccgcg caggcgggaa tctaagtctc gaattttcag
     1501 gaatgcctag gaggctccag aaatcccaaa tctccggatt tccacttgga caggaatgag
     1561 aaaaccggtc gtatttttta tctgcattaa tcattcatta aaggattgaa tattaaactg
     1621 aaaaccttgt tattgccctt cgccacgctg gcattgtgca ccaatgcttt tgccgccccg
     1681 cccagcgacg cgtcgttggc gcgttggctg gatacgcaga attttgaccg ggatatagaa
     1741 aaaaatatga ttgagggctt taatgccgga tttaaaccgt atgcggacaa agcccttgcc
     1801 gaaatgccgg aagcgaaaaa agatcaggcg gcagaagcct ttaaccgtta tcgtgagaat
     1861 gttttgaaag atttgattac gcccgaagtg aaacaggctg tccgcaatac tttattgaag
     1921 aatgcccgtg agatatacac gcaagaagaa attgacggca tgattgcctt ttacggttcg
     1981 cctgtcggtc agtccgtcgt tgccaaaaat ccgcgcttaa tcaagaaatc gatgagtgaa
     2041 atagcggtat cttggactgc attgtcaggg aaaatcgcgc aacatcatct gcccgagttt
     2101 acggaagagt tgcggcgcat catctgcggc ggtaaaaatc ccgatgcggg ctgtaaacaa
     2161 gccggacagg ttgggaaaag gcatcagaaa taaatgatag ccgtctgaaa tattgaagag
     2221 ggcatccgat tgattgaacc atcaaacccg aaagcaaccc tatggaaaaa aaattcttag
     2281 acatcctcgt ctgccccgtt accaaaggca ggctggaata tcatcaggac aaacaggaat
     2341 tgtggagccg tcaggcgaag cttgcctatc cgattaaaga cggcattccc tatatgctgg
     2401 aaaacgaagc gcgagcgttg agcgaagagg aactcaaagc atgaccgaat tcgtcgtatt
     2461 gattccggcg cggctggatt cgtcgcgcct gcccggaaaa gccttggcgg acatccacgg
     2521 caaaccgatg gtcgtgcgcg ttgccgaaca ggcggcaaaa agtaaagccg cgcgcgtcgt
     2581 cgttgccacc gaccatcccg atattcagac ggcctgtcag gcgcacggta tcgaagtcgt
     2641 catgacttca aaccggcacg aaagcggcac gacgcgcctt gccgaagcct ctgtcgcgct
     2701 gaagctgccg ccgcatttga ttgttgtgaa cgtacagggt gacgagccgc tgattgcccc
     2761 cgaactcatc gaccgcaccg ccgaagtact cgtcgaaaac aacgtccaaa tggcgaccgc
     2821 cgcccacgaa ttgcacgatt tcgacgaatt gatgaatccc aacgccgtca aagtcgtcct
     2881 cgacaaaaac cgcaacgcca tctacttcag ccgcgccccg attccctatc cgcgtgatgc
     2941 gatacgtgcc ggaaaacgcg aaatgccgtc tgaaaccgcc gtcctgcgac atatcggcat
     3001 ctacgcttac cgcgccggct tcctgcaacg ctatgccgaa atgagcgttt cgccgctgga
     3061 aaccatcgaa tcgctggaac agctgcgcgt cctgtggcac ggttatccca ttgccgtcga
     3121 aaccgccaaa gaagcccccg ccgccggtgt ggatacgcaa gaggacttgg acagggttcg
     3181 cgccgtattt cagaccgtat aaaacaggtt caaagggaaa agatatgcag caacatattg
     3241 aaaagtggca acacttgagc cgggaagaac agaaaatcct tgctgaagta tggggtctcg
     3301 tgcaaaacga cgatcaggag gttcactatg aaatgctcaa attgaacgca cccgatgaag
     3361 ccagcggcga attttggttc agaatggcag aaacactcag caccctgccg cccaaccgtt
     3421 ccctcggcct tagaatgaac ggcggcaggc tggcgaccgc cgtatccatc ctttccgtca
     3481 tgattgaaga caatcccgac ataccgcagc tttgggcgca aaaaattacc gcgctcaatt
     3541 atagtggatt aaatt
//
LOCUS       DAWGRZ010000181         3551 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000181 DAWGRZ010000000
VERSION     DAWGRZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.181"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(198..431)
                     /locus_tag="WHT44_001836"
     CDS             complement(198..431)
                     /locus_tag="WHT44_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533975.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESVFLSFSHFR"
     gene            complement(461..3226)
                     /locus_tag="WHT44_001837"
     CDS             complement(461..3226)
                     /locus_tag="WHT44_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ2533976.1"
                     /translation="MRSSFRLKPICFYLMGVTLYHYSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQEGALKFNSDSGKWER
                     DLQRQQWKYKPYKNYNNQELQKYIEEHDKSWRENLAPQYDITPIDPSSLKQQSAGNLF
                     KLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQK
                     YPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKNR
                     FPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALKK
                     DIYRLNYSTNTVGYRFGGEYTGYYGSDDEFKRAFGENSPTYKKHCNRSCGIYEPVLKK
                     YGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPERA
                     NTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVSSTG
                     LAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNAS
                     KEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYID
                     GTNGGNTSNFRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRYI
                     DPLDAGNDAATQRYYSSFDPKDKDEDVTCNADKTLCNGKYGGTSKSVLTNFARGRTFL
                     MTMSYKF"
BASE COUNT          838 a          865 c          871 g          977 t
ORIGIN      
        1 aaaagcagaa accgctccgc cgtcattccc acgaaagtgg gaatctagaa atttaatgtt
       61 gcggcactaa ccaaaaaaac cgaaaccgaa cggtctagat tcccgcctgc gcgggaatga
      121 cgaatccatc cgtacggaaa cctgcaccac gtcattctca cgaaagtggg aatccggaaa
      181 gctaaagcca caggaattta tcggaaatgg ctgaaactta aaaaaactga ttcccgcttt
      241 tgcgggaatg acgactcata aattacccga aacaaccaaa aataaccgaa atcaaaaacc
      301 ggcttccctg cgtgggaatg aaatggattt ttctttgagt tgccgatgcg ataaaacgga
      361 ttggcatagg cttgcggcag gatgatgcgt ttggggaaac aaatgccgtc tgaagggctt
      421 tcagacggca ttgcggtttt ctacaaaatg cgggctgcct ttaaaacttg tagctcatcg
      481 tcatcaaaaa ggtgcgtccg cgtgcaaaat tggtcaatac gcttttgctt gtgccgccgt
      541 atttgccgtt gcacaacgtt ttatcagcat tacacgttac gtcttcgtcc ttgtctttcg
      601 ggtcgaacga gctgtaataa cgctgcgttg ccgcatcatt gcccgcatcg agcggatcga
      661 tataacgcct gtcgaacaga tttttgactt cggcgcggaa aataaggttt ttcttcggct
      721 cgtaagcggc gtaaaaatca aaaatcaaag gctggcgggc aagagtttcg gtttgtttga
      781 tggaacgctt gcccagttgc cggaaattgc tggtatttcc cccgttggtg ccgtcgatat
      841 agcgttcttc agccgtcgcg cggatgctct tgccgaaata gcgcatcgcg ccgcccaaag
      901 tcagtttgtt gcccaaccag cgcgtaccga cttccaaacg tccgtaatct cgcggcaggg
      961 cggaaaccct gctcaaccca taaccttgtt tgagttggtc ttctttggac gcattgttgg
     1021 gcgattcgct cgcatcgctg aagttggtcg gttgcgtgct tttttgatag gcgtaagaaa
     1081 ggttggtgaa aaaacgccca taatcgtaat tcagctccaa ctcaaaaccg tgtttgtgca
     1141 ctttgtcttt gaaattgcga tgttggatgg tgtaggcaag cccggtgctg ctgacccagc
     1201 tcggaatatc cccgttcaaa tcccaccatt tcccgtaaac gttgtggatg tagttgtcga
     1261 tgcggctgcg gtagccgacc agttttaatc ctaatgtatc atcttgtttt aacaatcctt
     1321 ttttataggt attgaagcca aattgccaag tgtttgcgcg ctctggtttt aaggcggtgt
     1381 gaacgccgga gtcgccgatt tgggaaaaat acatttcttg gatgttgggc atacggtgtg
     1441 tgcgcgaata gctggcgaac ggcatgaaat aatcgccgaa gtccgcacta atgctgaccg
     1501 aatggttgtt ggcgcgcttt ttgccgtatt ttttcaatac gggttcataa atcccgcagc
     1561 tccggttgca atgtttcttg tatgtcggcg agttttctcc gaatgcccgc ttaaattcgt
     1621 catccgagcc gtaatagccc gtatattcgc cgccgaaacg gtagccgacg gtattggtgc
     1681 tgtagtttaa gcggtaaatg tcttttttga gcgcggcatc gaagtagaac gtgttgaaat
     1741 attggctgcc ggccggttgg acaatggttg atttttgggg cagcagccct ttatcgccct
     1801 taaaccgccc caaataggaa taaagcccgt tgtcctgatc aggaccgtcg aaaaacagcc
     1861 ccaattcttc aggaaagcgg tttttgccgt attcgttgtg gaaataattg aagcccaaag
     1921 tggtttgcaa ctcggtttcg cggggcagcc ggaaggtggc ggtgttgttg aggtcgagga
     1981 ttttcgcgtt gttgtaggtt tcaaaatcct ttaaaagccc ccagcctgta aacttcgacc
     2041 ctttcggata tttctgcctg cccgaattgt aggctgcggt cagattgagg ttggtatacg
     2101 ggttcaaaga caaaccgtaa ttgaactgat aattgcggtt gatgattttg cggctgccga
     2161 ttttggtgtt taaatcgcga aattgcgccg tgtatttatt gaatacgccg tcgtattcca
     2221 atttaaacag attgcctgcc gactgctgct tcaggctgga cggatcgatg ggggtaatgt
     2281 cgtattgcgg tgccaggttt tcccgccagc ttttgtcatg ctcttcgatg tatttttgta
     2341 gttcttggtt gttgtaattt ttatacggct tgtatttcca ctgttgcctt tgtaaatccc
     2401 gctcccattt tccgctgtcg gaattgaatt tcaaagcacc ctcttgtaca aaatatcgct
     2461 gcttgcgccg ttccaaatat tccgcgccaa aatttccgat gtgctgcccg ccgccgccca
     2521 cgcggtaatt ttgcgccacg ctgcgcctgc tgtgcccgta aagcacaccg acagatgctc
     2581 cgctttccag ccatttgcgc gcacctatcg ccgccatcgc attacctttg gttgaattgg
     2641 tgccggtcag accttttagc agcaggccgt aggtattatt gccctgaacg acgtcatcca
     2701 cgcctaaagt ccgcagattc gccgaaccgg caaggctgtt gatgcctgcc gagccgctga
     2761 agctgccttt gacgacatcc agtccggcaa taaaattgct gtcgacagat gcaccgaatt
     2821 gagatgaacc gcctgccctg cccgcatcgg tagaagtcga ataaaaggtc tgcgtgatgc
     2881 cgtccaccat cgtattgacc cgcccgaacc cgctgtcgcc gcgaatattc aaagacacaa
     2941 tgcccgagct tttatcttgc tgtgtaaacg caccggggat gctgcgtacg atgttgtcga
     3001 ggttttcgct ggatttgaat atatcctgac gggtcgatac ggcacgcgca tcggtaaaca
     3061 cttttttgtc tttcggtacg cgcttcgcct tgacgtgcac atcttccaaa acctgtatct
     3121 gcgcctcgct gcccgcgcgc cctgcatctt cggcataact ataatgatat agcgtaacac
     3181 ccataaggta aaaacaaatc ggcttcaacc ggaaagaaga tctcatattt tcctcaacaa
     3241 taaacagtca gacaattagg aaatatacta gcattttttt caggcggcat aaacattaaa
     3301 aaagtgtaaa tttgatatac cgtctgaaga tttcaattgg atatttatag tggattaaca
     3361 aaaaccagta cagcgttgtc tcgctttagc tcaaagagaa cgattctcta aggtgctgaa
     3421 gcaccaagtg aatcggttcc gtactgtttg tactgtctgt ggctcgccgc cttgtcctga
     3481 tttttattaa tccgctataa agaccgtcgg gcatctgcag ccgtcattcc cgcgcaggcg
     3541 ggaatctagt c
//
LOCUS       DAWGRZ010000182         3547 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000182 DAWGRZ010000000
VERSION     DAWGRZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.182"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            1..1775
                     /locus_tag="WHT44_001838"
                     /pseudo
     CDS             1..1775
                     /locus_tag="WHT44_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            1899..3449
                     /locus_tag="WHT44_001839"
     CDS             1899..3449
                     /locus_tag="WHT44_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ2533977.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQETGFL
                     TVESHQYERGWFTSMETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAYIETEFKYAPETEKVLERFFGKQVPASLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGETVYQKGFKSYRNGYDAPLFKIKLADKGDAAFEKVHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFINSEGQFRFDTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTLPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT          995 a          875 c          905 g          772 t
ORIGIN      
        1 ggcaaagcca acgcctttac cgcgccgatt accgtcgtca actacgacga acaagccctc
       61 aacaacaccg aggcgcgtac tttggtggat gccgtagcga aaaaagacgc ttccgtttgg
      121 cagttcggcg gcgaaagcaa cacgctgacc ggattgtatt tcagaggtta tcagcttgat
      181 gcgcgccaat tcagcgtcaa cggtttgaca ggtatgtacg gcacgcaagg cacgaccagc
      241 gtgcaagtcg gctccgcaca actgattcaa ggcgcgtcca ccgccgtaaa cggcatgaac
      301 cctgatggcg cggtatccgg ttccgtcaat atcgaaacca aaaaagccgc tgatgaaggc
      361 aaccgcaaaa tcggtttggc ccggttcggc aacaaccgcg cccaaggtac gttcgacttg
      421 ggccaacgtt tcggcgaaaa caaagcattc ggcgtgcgcg ccaacggcaa actgcgccac
      481 ggcgacaccc cgcgccacgg ttacagcgaa gacaacaaag aatttgcggt aaatgccgac
      541 tatcgcggcg aaacactgcg cgtagcgttc gattccatct acgcaaaacg caaaaccaac
      601 ggcggtcgcg cgcgtatgca ggacattcaa aacgccggcg gacgcttgtc ctgactttgg
      661 gcggacgttt ccaagcggtt gaatacacca ataagaaaaa atcacgcgga acggcagccg
      721 gagctagcaa ggtagaagtg aaggcaaacg gaaatattga gcatcaattg gcatcgttta
      781 cagccgacta tcccaattct tgggagaact ctgccaactt ggatgccaat atcaaagtca
      841 acagcctggc tttgtccgac accttgggct tcgtggataa caaataccgc ctgactttgg
      901 gcggacgttt ccaaacagtc gaatataccg acaaaaaatc acagagcggc gatgccaaac
      961 gtttcagccc gatgctgatg gcggcgtggg tgccgcaacc tgatttggtt gtgtacggca
     1021 actatatgga agacttggag cctgccgaca tcaaaaccga tgattccggc gaaacgacaa
     1081 tggcgaaacc gcgtgtcagc cgccagtttg aagtgggcgt gcgtaaaaac tggggcaatt
     1141 tcgtaaccac gttaaacgcg ttccaaatca aacgcccggg ctactggcgc ggtcatacaa
     1201 ctaccaagac aacaaaaggg gtaacaacaa cgctgaccta tggcaataat tctgatttcg
     1261 cccgctataa ggcgcaaggc ggcgcggccg gcgacgagca aggtatggaa cgcaatcgcg
     1321 gtattgagtt caacacctac gcgaacttgc tcaacaatac tttgcgtccg actttgggtc
     1381 tgatgtatct gcaatcgacc gtgaaagagt atccaaattc acgcgatatg ctggttaacg
     1441 gcgtacaagt cgccaatccg cgcgtaattg ccaaagcagg tttggaatgg gatacaccgt
     1501 ttgccaaagg cttgacttta aacggtaatg tttcgtattt cggcaagtct gaccaagaca
     1561 cgcaaaaaca atacgccttc ccgtcctata ccttgattga cgtaggcgcg cgctacaaaa
     1621 ccaagcttgg caaaaacact ctgaccgtca gcagctcggt ggaaaacctg ttcaacaaaa
     1681 actattggca ggtacagcgc ggccaatacg accgcagctt cgccgtcgtc ggtatgccgc
     1741 gcacttactg gctgaaggcg gaattggatt tctgatttcc ctatatccgg aaaatgccgt
     1801 ccgaacaggt ttcagacggc atttctttta aagagatatg gcgggatacg cttaaataat
     1861 gctattctag cgtctttgaa acctgcttgt ttgaagtgat gaaaaaacct ttgatttcgg
     1921 ttgcggcagc attgctcggc gttgctttgg gcacgcctta ttatttgggt gtcaaagccg
     1981 aagaaagctt gacgcagcag caaaaaatat tgcaggaaac gggcttcttg accgtcgaat
     2041 cgcaccaata tgagcgcggc tggtttacct ctatggaaac gacggtcatc cgtctgaaac
     2101 ccgagttgct gaataatgcc cgaaaatacc tgccggataa cctgaaaaca gtgttggaac
     2161 agccggttac gctggttaac catatcacgc acggcccttt cgccggcgga ttcggcacgc
     2221 aggcgtacat tgaaaccgag ttcaaatacg cgcctgaaac ggaaaaagtt ctggaacgct
     2281 tttttggaaa acaagtcccg gcttcccttg ccaataccgt ttattttaac ggcagcggta
     2341 aaatggaagt cagtgttccc gccttcgatt atgaagagct gtcgggcatc aggctgcact
     2401 gggaaggcct gacgggagaa acggtttatc aaaaaggttt caaaagctac cggaacggct
     2461 atgatgcccc cttgtttaaa atcaagctgg cagacaaagg cgatgccgcg tttgaaaaag
     2521 tgcatttcga ttcggaaact tcagacggca tcaatccgct tgctttgggc agcagcaatc
     2581 tgaccttgga aaaattctcc ctagaatgga aagagggtgt cgattacaac gtcaagttaa
     2641 acgaactggt caatcttgtt accgatttgc agattggcgc gtttatcaat cccaacggca
     2701 gcatcgcacc ttccaaaatc gaagtcggca aactggcttt ttcaaccaag accggggaat
     2761 caggcgcgtt tatcaacagt gaagggcagt tccgtttcga tacactggtg tacggcgatg
     2821 aaaaatacgg cccgctggac atccatatcg ctgccgaaca cctcgatgct tctgccttaa
     2881 ccgtattgaa acgcaagttt gcacaaattt ccgccaaaaa aatgaccgag gaacaaatcc
     2941 gcaatgattt gattgccgcc gtcaaaggag aggcttccgg actgttcacc aacaatcccg
     3001 tattggacat taaaactttc cgattcacgc tgccatcggg aaaaatcgat gtgggcggaa
     3061 aaatcatgtt taaagacatg aagaaggaag atttgaatca attgggtttg atgctgaaga
     3121 aaaccgaagc cgacatcaga atgagtattc cccaaaaaat gctggaagac ttggcggtca
     3181 gtcaagcagg caatattttc agcgtcaatg ccgaagatga ggcggaaggc agggcaagtc
     3241 ttgacgacat caacgagacc ttgcgcctga tggtggacag tacggttcag agtatggcaa
     3301 gggaaaaata tctgactttg aacggcgacc agattgatac tgccatttct ctgaaaaaca
     3361 atcagttgaa attgaacggt aaaacgttgc aaaacgaacc ggagccggat tttgatgaag
     3421 gcggtatggt ttcagagccg cagcagtaat gggtcaaatg ccgtctgaaa tgttcaggcg
     3481 gcattttgtt gtattggaaa ctgccgtttt caaccattcc tttagataag ccgtgtcgga
     3541 ttcgttt
//
LOCUS       DAWGRZ010000183         3541 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000183 DAWGRZ010000000
VERSION     DAWGRZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.183"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            185..1060
                     /locus_tag="WHT44_001840"
     CDS             185..1060
                     /locus_tag="WHT44_001840"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ2533978.1"
                     /translation="MLQGSLVALITPINQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGIYQHFKTIAEATSIPMIIYNVPGRTVVSMTNDTILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEN
                     GQAKVRAALKASGQL"
     gene            1069..2265
                     /gene="bamC"
                     /locus_tag="WHT44_001841"
     CDS             1069..2265
                     /gene="bamC"
                     /locus_tag="WHT44_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ2533979.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDKVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTVLALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(2322..3254)
                     /gene="pip"
                     /locus_tag="WHT44_001842"
     CDS             complement(2322..3254)
                     /gene="pip"
                     /locus_tag="WHT44_001842"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ2533980.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETVWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIRHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLVDALVQAVEDILPHLL"
BASE COUNT          946 a         1168 c          783 g          644 t
ORIGIN      
        1 cgacggcacc ggcataaacc ggcgaatata ataaaatgat ttcaaacagg aaaaatcaat
       61 ttccaccata cctttcggcg caaacgcccc acatctgcaa cccgccggat ttcacacctg
      121 cggtaaagta ggctacaatc ggaagcatta ccgcccctac accacaactg ataaaaggtt
      181 ctgtatgtta caaggtagct tggttgccct gattaccccg ataaatcaag acggcagcat
      241 ccattacgaa caactccgcg acttaatcga ctggcacatt gaaaacggca cggacggcat
      301 cgttgccgtc ggcacgacag gcgaatccgc caccctctcc gtcgaagaac acaccgccgt
      361 catcgaagcc gtcgtcaaac acgttgccaa acgcgtcccc gtcatcgccg gcacaggggc
      421 aaacaacacc gtcgaagcca tcgccctttc ccaagccgcc gaaaaagccg gggcggacta
      481 caccctctcc gtcgtcccct attacaacaa gccctcccaa gaaggcattt accaacattt
      541 caaaaccatc gccgaagcca cttcgattcc gatgattatc tacaacgtgc ccggccgtac
      601 cgtcgtcagc atgactaacg acactatcct gcgccttgcc gaaatcccca acatcgtcgg
      661 cgtgaaagaa gccagcggca acatcggcag caacatcgaa ctcatcaacc gcgcgcccga
      721 aggcttcgtc gtcctttccg gcgacgacca caccgccctg ccgttcatgc tctgcggcgg
      781 acacggcgtg attaccgtag ccgccaacgc cgcgcccaag ctctttgccg atatgtgccg
      841 cgccgcgctt caaggcgata tcgcccttgc acgcgaactc aacgaccgac tgattccgat
      901 ttacgacacc atgttctgcg aacccagccc cgccgcaccg aaatgggcgg tatccgccct
      961 gggcagatgc gaaccgcacg tccgcctgcc gctcgttccg ctgacggaaa acggacaagc
     1021 aaaagtccgc gccgccctga aagcctcagg acaactttaa atatagccat gccgtccgaa
     1081 ccgttcggac ggcataacgc aacaaacact ttaatatcca tcacacagga tgacacgatg
     1141 acccatatca aacccgtcat tgccgcgctc gcactcatcg ggcttgccgc ctgctccggc
     1201 agcaaaaccg aacagcccaa gctcgactac caaagccggt cgcaccgcct gatcaaactt
     1261 gaagtcccac ctgatttgaa caaccccgac caaggcaacc tctaccgcct gcctgccggt
     1321 tcgggcgccg tccgcgccag cgatttggaa aaacgccgca cacccgccgt ccaacagcct
     1381 gccgatgccg aagtattgaa aagcgtcaaa ggtgtccgcc tcgagcgcga cggcagccaa
     1441 cgctggctcg ttgtcgacgg caagtctcct gccgaaatct ggccgctcct gaaagccttt
     1501 tggcaggaaa acggcttcga catcaaatcc gaagaacccg ccatcggaca aatggaaacc
     1561 gagtgggcgg aaaaccgcgc caaaatcccc caagacagct tgcgccgcct cttcgacaaa
     1621 gtcggcttgg gcggcatcta ctccaccggc gagcgcgaca aattcatcgt ccgtatcgaa
     1681 cagggcaaaa acggcgtttc cgacatcttc ttcgcccaca aagccatgaa agaagtgtac
     1741 ggcggcaaag acaaagacac gaccgtatgg cagccctccc cgtccgatcc caacctcgaa
     1801 gccgctttcc tgacgcgctt tatgcaatat ttgggcgttg acggacagca ggcggaaaac
     1861 gcatcggcaa aaaaacctac ccttcccgcc gccaacgaaa tggcgcgtat cgaaggcaaa
     1921 agcctgattg tctttggcga ctacggcaga aactggcggc gcaccgtgct cgccctcgac
     1981 cgcatcgggc tgaccgtcgt cggtcaaaac accgaacgcc acgccttcct ggttcaaaaa
     2041 gccccgaacg aaagcaatgc agttaccgaa caaaaacccg gcctgttcaa acgcctgctg
     2101 ggcaaaggca aagcggagaa acctgccgaa cagccggaac tgattgtcta tgcagaacct
     2161 gtcgccaacg gctcgcgcat cgtcctgctc aacaaagacg gcagcgcata tgccggcaaa
     2221 gacgcatccg cattattggg caaactccat tccgaactgc gttaaaccat tcaaaacaaa
     2281 agcagcttcc aaacagaagc tgctttttta tgcggaactt tttacaacaa atggggcaaa
     2341 atatcctcaa ctgcctgaac caacgcatcc accaaaggcg gatcgaacgc acgatgccct
     2401 gcctgaacca ccctcaattc cgcttcggga aaggctttcg acagcgccca cgcactctgc
     2461 atcggcgtac acaaatcata ccgcccctgt acgataatag tcgggatatg ccgtattttg
     2521 ccgatattgt tcaaaatcgc cctatcgccc tgcaaccaac cgccgttgac aaaataatgg
     2581 ttttccaaac gcgcgattgc cagcgaggca taagcatctt catccacttc ctccggctcg
     2641 aaacggatca gatagctttc ccaatccgcc caagccttcg cggcagacag gcacacttct
     2701 tcatcttgat gaaacagcaa tccgtgatac gcctcaatca gccggttccg ccgattttca
     2761 gcaatcggcg cgacaaattt ttgccattgt tccggataaa tccggctcac accgcccgcc
     2821 tcgttcagcc acaccgtttc agacggcctg cacaaaaata tcccgcgcaa caccaatccc
     2881 tttacccgtt caggatgggt ttgggcataa gccagcgaca aagtgctgcc ccacgaaccg
     2941 ccgaacacca gccatttccc gatacccagc atttcacgga ctttttcaat atccgccacc
     3001 aaatcccaag tcgtattgtc ttccgcacaa gcatacgggc gcgaacgtcc gcaaccgcgc
     3061 tggtcgatga tgacgatgcg gaacacatcg ggattgaaaa aaccccgaca ttcaggcgaa
     3121 gcccccgcgc ccggcccgcc atgtaaaaaa ataaccggca caccgtcggg attgcccgat
     3181 tcctcccaat aaatttgatg aatttcagac acctgcaagt atccgctatg aaaaggctgt
     3241 tttatctcat acatcatctt ctcccatatt gcttctgaca atccgaacat tcccacacgc
     3301 aacatcacgc acgagacaca agccgccccg aaaggcaaat gccgaaaaat cgcgatatag
     3361 tgaattaaca aaaatcagga caaggcggcg agccgcagac agtacggata gtacggaacc
     3421 gattcactcg tgcttaagca ccttagagaa tcattctctt tgagctaagg cgaggcaacg
     3481 ccgtactggt ttttgttaat ccactataac agcaaccctg tcgccgtcat tcccgcaaaa
     3541 g
//
LOCUS       DAWGRZ010000184         3487 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000184 DAWGRZ010000000
VERSION     DAWGRZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.184"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            100..1215
                     /gene="lst"
                     /locus_tag="WHT44_001843"
     CDS             100..1215
                     /gene="lst"
                     /locus_tag="WHT44_001843"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ2533981.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKEKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKTGDETGGTVRILLGSPDKEMKEISEKAAKNFKIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKN"
     gene            complement(1380..1838)
                     /locus_tag="WHT44_001844"
     CDS             complement(1380..1838)
                     /locus_tag="WHT44_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ2533982.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PEFTRMGKMVKDEEPYDVEKFKQAAASFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            1957..2778
                     /locus_tag="WHT44_001845"
     CDS             1957..2778
                     /locus_tag="WHT44_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ2533983.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDERAAGVYEGVEFIRPEDIAETALW
                     LYQRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            complement(2829..3275)
                     /gene="yciA"
                     /locus_tag="WHT44_001846"
     CDS             complement(2829..3275)
                     /gene="yciA"
                     /locus_tag="WHT44_001846"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ2533984.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVDVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLAGLLPTP"
BASE COUNT          822 a          753 c         1007 g          905 t
ORIGIN      
        1 tattcggtaa ctgtcggaat atctgctaaa attccgcatt tttccgtccc gggacactcg
       61 gggcgtatgt tcaatttgtc ggaatggagt tttagggata tgggcttgaa aaaggcttgt
      121 ttgaccgtgt tgtgtttgat tgttttttgt ttcgggatat tttatacatt tgaccgggta
      181 aatcaggggg aaaggaatgc ggtttccctg ctgaaggaga aacttttcaa tgaagagggg
      241 gaaccggtca atctgatttt ctgttatacc atattgcaga tgaaggtggc ggaaaggatt
      301 atggcgcagc atccgggcga gcggttttat gtggtgctga tgtctgaaaa caggaatgaa
      361 aaatacgatt attatttcaa tcagataaag gataaggcgg agcgggcgta ctttttccac
      421 ctgccctacg gtttgaacaa atcgtttaat ttcattccga cgatggcgga gctgaaggta
      481 aagtcgatgc tgctgccgaa agtcaagcgg atttatttgg caagtttgga aaaagtcagc
      541 attgccgcct ttttgagcac ttacccggat gcggaaatca aaacctttga cgacgggaca
      601 ggcaatttaa ttcaaagcag cagctatttg ggcgatgagt tttctgtaaa cgggacgatc
      661 aagcggaatt ttgcccggat gatgatcgga gattggagca tcgccaaaac ccgcaatgct
      721 tccgacgagc attacacgat attcaagggt ttgaaaaaca ttatggacga cggccgccgc
      781 aagatgactt acctgccgct gttcgatgcg tccgaactga agacggggga cgaaacgggc
      841 ggcacggtgc ggatactttt gggttcgccc gacaaagaga tgaaggaaat ttcggaaaag
      901 gcggcaaaaa acttcaaaat acaatatgtc gcgccgcatc cccgccaaac ctacgggctt
      961 tccggcgtaa ccacattaaa ttcgccctat gtcatcgaag actatatttt gcgcgagatt
     1021 aagaaaaacc cgcatacgag gtatgaaatt tatacctttt tcagcggcgc ggcgttgacg
     1081 atgaaggatt ttcccaatgt gcacgtttac gcattgaaac cggcttccct tccggaagat
     1141 tattggctca agccggtgta tgccctgttt acccaatccg gcatcccgat tttgacattt
     1201 gacgataaaa attaatcgca tagcaaatca aaatagaaaa tggcggagtg cgtggggtaa
     1261 aaataaggat agcgtttttt catttggatt gacgataatt tctgattgct ttgcgtgtgc
     1321 tgaaatggca aagaaaatgc cgtctgaagt cttcagacgg cattgttttg ttttggatgt
     1381 tattcgggcg cgcggaaact gtcgtggcag gatttgcagc ttgcgccggt ttcgccgtag
     1441 gcggctttga tttcgtccag tttgccggtt tgggcggcgg cgttgagttt ttcgacggcg
     1501 gcggcgaatt ttgttttttc ggcttcaaat tttgcaccat ccgaccaaac ggcgggcagt
     1561 gcgcggccgt tgccttgcgg atcggactca aaaagtgtga acggtttctt gctgctttcg
     1621 gcaaacgacg ctgccgcctg tttgaatttt tcgacatcgt aaggttcttc gtctttgacc
     1681 attttgccca tgcgtgtgaa ttcgggcatc atggatttga acgcggcggt gcggttttcg
     1741 gaaatttcgc ctttgggttg ggaaggtatt ccgctgcctc cgcaggcgga aaggagcagt
     1801 gtgatggcgg cagcagcaag gctgatttgg gttttcatat tgaatgtgtc ctgtcgtggt
     1861 ggtatggttg ttgtcatttt cagtcggcgc gatgccgtct gaagggtgtt atgatacctg
     1921 aaacaggttt gggaaacgag aaagaaagga aaaacaatgg ctgttttaat taccggtgct
     1981 tcggcaggat tcggcgaagc gatgtgccgt gcgtttgtcg gggcgggata ccgcgttatc
     2041 ggtgcggcgc gccgtgcgga caggcttcag gccttggcgg atgaattggg tgctttgttt
     2101 taccctttgg aaatggacgt gtcgcgacgc gagtcggtgg aaaacgcctt aaacggcatc
     2161 cccgatgaat tttccgacat cgactgcctc atcaacaatg ccgggctggc tttgggtctg
     2221 gacacggcgg acaaggcgga ttttgaagat tgggaaacga tgattcaaac caatgttttg
     2281 ggtttgacgt tcctgacgcg caaaattttg ccgcaaatgg tggaacgcgg cggcggttat
     2341 gtgatgaatt tgggttcgat tgcaggcaat tatgcttatg ccggcagcaa cgtttacggg
     2401 gcgaccaagg cgtttgtgcg ccagttcagc ctgaatttgc gcgcggagtt ggcggataag
     2461 aacatccgcg ttaccaatat cgagccgggt ttgtgcggca atacggagtt ttccaatgtg
     2521 cgcttcaaag gcgatgacga gagggcggcg ggcgtgtatg agggtgtgga atttatccgc
     2581 cccgaagata ttgcggaaac cgcattgtgg ctgtaccagc ggccggcgca tatgaatgtg
     2641 aacacgattg aaattatgcc cgtggcgcag acttttgcag gaatgaaagt gataaaaaaa
     2701 gccgtgcccg aagtgcggga agactttgaa aaacagagta tgtcgctgtt ttcccgcatc
     2761 aggtcctggt tcaaatgatg caaaatgccg tctgaagaca gtttcagacg gcatttttac
     2821 gggtattttt acggagtagg caataagccc gccaatttgg ggttgccttc tttcggaatc
     2881 gggcgcggat tgccttccgc atcgatggca acataagtga acacggcttc ggttacgagg
     2941 tagcggtctt cggtaacgca atcgttcatc aaagttttca cccagacgtc gactttaagc
     3001 tggagggaag tgttgcccac gcggacgcaa tgcccgtagc agcagacgac gttgccgacc
     3061 ttgaccgggc ggatgaagtt catttcctga acggcgacgg taacgatgcg tccccgcgcg
     3121 atttccgccg ccaatatgcc gccgcccaaa tccatctgcg acataatcca gccgccgaaa
     3181 atgtcttggt tgggattggt atcgcgcggc atagcgacgg tacgcaggag cagttcgcct
     3241 tgagggcgtt ggcggttgcc ttcttcgtgc tgcataaagt ttccttgttt tattgaaata
     3301 taaatcgaac ctgcacccct gcccgaaacg ggaagtcggg aagtgtgtag tttaacccat
     3361 ttgagacata caagggcggg cgcggaacag attcgcaagg cgtattgtag ggcggggctg
     3421 tagagtgggc ttcagtccgc caatcccgcc aaatcctacc ctaagcaact gaaccgtcat
     3481 tcccacg
//
LOCUS       DAWGRZ010000185         3473 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000185 DAWGRZ010000000
VERSION     DAWGRZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.185"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(493..599)
                     /locus_tag="WHT44_001847"
                     /pseudo
     CDS             complement(493..599)
                     /locus_tag="WHT44_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1018..1788)
                     /gene="xth"
                     /locus_tag="WHT44_001848"
     CDS             complement(1018..1788)
                     /gene="xth"
                     /locus_tag="WHT44_001848"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ2533985.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRSVPQDVHFGLPALPDDPQRRVIAATVSGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRHGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(1848..2129)
                     /locus_tag="WHT44_001849"
     CDS             complement(1848..2129)
                     /locus_tag="WHT44_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ2533986.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSNHLNRLRVEGLVDFTRYHRIIEYRLVSEEAAAILHTVRDLENKRVA"
     gene            complement(2221..2508)
                     /locus_tag="WHT44_001850"
     CDS             complement(2221..2508)
                     /locus_tag="WHT44_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533987.1"
                     /translation="MSGKEPLNPNPVIASGRKCRLKDSSDGIFRKGRDALEKTAFQAG
                     ILFARQRGGIGRAGKRTGGGSFMPSESPPLRLFAKKVGKGTYNPVQSSINI"
     gene            complement(2536..3417)
                     /gene="nadC"
                     /locus_tag="WHT44_001851"
     CDS             complement(2536..3417)
                     /gene="nadC"
                     /locus_tag="WHT44_001851"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ2533988.1"
                     /translation="MPSEKTLFPLPDTLLRPIVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVQQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTIYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
BASE COUNT          848 a          825 c          874 g          926 t
ORIGIN      
        1 ctgatgcaga tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta
       61 caaatagtac ggcaacgctg tactggttta aatttaatcc actatatttt gtaatagctg
      121 ccattatgcc tactaggata tataccttct atataatcct tatttaaata aaaaccctta
      181 taaaaatctg tgttatctgt cgggattgaa aaaaagattt tatctttctc atcaggttta
      241 tatttgctat caatatcaaa aagagaataa ggattacaat gagttcgaca tactaggtaa
      301 aaatttatat cttggatcat gaaatattcg atatttatca ggatcgaaag atgctaaaaa
      361 ttcatcaata ttggtactgg atattttgtc atcactaata aacatcgcta gagatgggcg
      421 actttgattt tccatttgtt gattagtaaa ggatataaat actaacttgc cattgtcaaa
      481 ccacatagat ttatatagtg gattaaattt aaaccagtac gtcgttgcct cgccttgccg
      541 tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatag
      601 ttattgcctt tgggagaatc aattctgaca gcacataaca tagatttatc tttacagtcg
      661 ttatattgag tttcatcaaa ctcaactgca tggcacaata aaggcaatag gaaaaacagg
      721 gttattaatt tattactata tataagacgg ctttctattt attcgaaaat aaatctagtt
      781 ttcaatattt gattttgttt tggattagtg tacataataa attttactgc tccaaaatct
      841 gatattaacc ctatgtattt cataaatttt tcaggaaaat aggctgtgtt tgaacaatcc
      901 gccgatttcg gctgtttgcc ggggaaaggc aattattttt cagtcagata gaaagctatc
      961 ctattgaata aattgaagat aaggatgctt gtcaacgcca tatcaaaaca cggtctttta
     1021 ccaatcgaat tctgccgtca ccggcgcgtg gtcgctcgga cgctccagcg cgcgcgtctc
     1081 caaatcgacg cggacatcct tcaacgccgc cgccatcgca ggcgacacca aaatatggtc
     1141 gatacgcagg cccagtttgc gttggaacat cgcgccgcga tagtcgaacc aggtatagaa
     1201 cgcgccttcg ggatggactt ggcgcaggct gtcggtcagt cccaaatcca gcaggttttg
     1261 aaaccactgc cgttcgacgg acgaacagtg gattttttcg tgccattttt cagggtcgta
     1321 acagtccgca tcggcaggcg cgatattgaa atcgcccagc aacaccagtt tgccgtggcg
     1381 ggtcatttca tcgcggacaa actccgtcag tgcggcaaac cactgttcct tatatttgaa
     1441 tttggggctg tccaaagcct cgccgttgac gcaatagaca ttgatgacgc gcacgccgct
     1501 gacggttgcc gcaatcacgc gccgttgcgg atcgtccggc agtgcgggca aaccgaaatg
     1561 cacgtcctgc ggcacgctgc ggctgacgat tgccacgccg ttgtaggttt tctgcccgct
     1621 ccaaacacag tgccagccca tcatttgcaa agcggcggcc ggaaatttgt cctgatcgag
     1681 tttgagttcc tgcaaaacca aaatatcggg cggattgtcg gcaagcaggt tttgcacctg
     1741 cggcagccgc acattgagcg aattgacatt ccaagtggtg attttcataa catttccgaa
     1801 aaatgctgtc tgaaacgttc agacggcaaa aggaaaggat tctaacacta tgccacgcgt
     1861 ttgttttcca aatcgcgaac cgtgtgcaga atcgccgccg cttcttcgga aaccaggcgg
     1921 tattcgataa tgcggtggta acgcgtaaaa tcgactagac cttccacgcg caggcggttc
     1981 aaatggttgg aaactgcggt ggccggcagg gataaggatt ttgccagttc ggcgatattg
     2041 cgttcgctgt ccaacaattg aatcagtatc gccatacgtt cgggatgcgc cataagtttg
     2101 agtatggtgt ggagcggtat tgtgttcatc ggtattccta aaacctttgc cgcccgccaa
     2161 gcggattgac gggcggcaga tatggatgat tgtgattatc tttataaatc gtattataaa
     2221 tcaaatattt atggatgatt gtacaggatt gtatgttcct tttcccactt tttttgcaaa
     2281 caagcgtaaa ggcgggcttt cagacggcat aaaactgccg ccccccgtcc tttttcctgc
     2341 cctaccgatg ccgccgcgct gtcgggcaaa caaaatacct gcctgaaatg ctgttttttc
     2401 taaagcatca cgacctttac gaaaaatgcc gtctgaagaa tctttcagac ggcatttcct
     2461 ccctgaggca ataaccggat tagggttaag cggttccttg cctgacatat cagacagccg
     2521 cccgcaccct aaaactcacg ccacgaaatc caaacctatg tccaacgaac ggctgctgtg
     2581 ggtcagatag ccgagggcga tgccgtccac tccggtttgc gccacgcgct tcaggcggtc
     2641 gaagccgatg ccgcccgatg cttcgcaata gatggtgtgg gggtgggcgg tttgcgtgtg
     2701 gcagcggttt gccgcttctt tcagggtttc gtcgtccatg ttatccagca aaatccgttc
     2761 cgcgcccgct gcgatggctt cgtccagttg tgccaacgta tccacttcga tttccacgca
     2821 ggtcaatgct ccgacagcct gttttgcctg ctgcacggct tgggcgatgc tgccgcaata
     2881 ggcgaggtgg ttgtctttga tgagcacggc gtcgtccaaa cccatgcggt ggttcacacc
     2941 gccgcctgcc ctgacggcgt atttttgcag gacacgcagc agggggatgg ttttgcggct
     3001 gcacacgatg tctgtaccgt attcggcgac ttcggcaacg gcacgcgcgg tggcggtggc
     3061 gatgccgctt aagtgcgtga ggtagttgag cgcggtgcgt tcggcggcga gcagcgcgcg
     3121 ggcgttgcct tcgacggcgg caagcgtctg acctgcgcgg acggcttgcc cgtctcggat
     3181 ttcggcttgg aagcggacgg acggatccat cgtctgaaag gcgagacgcg ccaagtccat
     3241 gccggcgata acgccgtctt cgcggctgac aaggaagagt ttggcggttt tgtcgggggc
     3301 gatgacggcg gcggacgtaa tatcgccgcg cctgcccaag tcttcgctca aggcttgttc
     3361 tactatgggg cgcaacaggg tgtcgggcag gggaaagagg gttttttcag acggcatggg
     3421 gtttcctttg ttgaaaaaga ttgatgttgc accgatgggg caaaacttca ttt
//
LOCUS       DAWGRZ010000186         3435 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000186 DAWGRZ010000000
VERSION     DAWGRZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.186"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..158
                     /locus_tag="WHT44_001852"
     CDS             <1..158
                     /locus_tag="WHT44_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533989.1"
                     /translation="LKFDQLILEFPERGDGAWVHIGFRRNSPQRNQILTATKKNGKTV
                     YLPGLHP"
     gene            170..679
                     /locus_tag="WHT44_001853"
     CDS             170..679
                     /locus_tag="WHT44_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2533990.1"
                     /translation="MDILLKYWKPVGVLLLIVLIFTAWHFDRAEKYRMGREAAAAEIS
                     NRLKDGYIEQAKQARSAEQKAAAAFAERQTKLEEEKQNAEKTVAAMRLELNRLRHYAA
                     AQNRNRNLPATATAATASDGASDSQGWLLFGQCAEKYAGLAETADGHAADLREWQAYG
                     HAVSSQRAE"
     gene            complement(732..1079)
                     /locus_tag="WHT44_001854"
     CDS             complement(732..1079)
                     /locus_tag="WHT44_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ2533991.1"
                     /translation="MYIPDKGDIFHLNFDPSSGKEIKGGRFALALSPKAFNRATGLVF
                     ACPISQGNAAAARSSGMISTLLGAGTETQGNVHCHQLKSLDWQIRKASFKETVPDYVL
                     DDVLARIGAVLFD"
     gene            complement(1081..1317)
                     /locus_tag="WHT44_001855"
     CDS             complement(1081..1317)
                     /locus_tag="WHT44_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEZ2533992.1"
                     /translation="MLRVQKWGNSAAVRLPADMLKQLDFKIGDALVAEVHNGELRVRA
                     ARRFRLADLLAEMEETPPRVEGWEILDDAGNEVV"
     gene            complement(1676..2167)
                     /locus_tag="WHT44_001856"
     CDS             complement(1676..2167)
                     /locus_tag="WHT44_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEZ2533993.1"
                     /translation="MFWQLTVVSVTAVIALGTIFINKKTSKQKATLDVILNDYQDAQF
                     VEADNHISPYIRGTAVDDNNARIDLYEIYQNKGGQWEKERGHLLTVINRHEFYACAIN
                     SGVLDEDLFKRLHCTNFIKLWNAVSPLVMKIREEERKDTIFRELEILVALWKANPLKA
                     SDL"
     gene            complement(2605..2973)
                     /locus_tag="WHT44_001857"
     CDS             complement(2605..2973)
                     /locus_tag="WHT44_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEZ2533994.1"
                     /translation="MIDGELVALIHQTVLADEAGLKGRADMARLDGALSRIANWRQYE
                     NLEDIYEIAALYAQAIAKAHAFPDGNKRTALLTMLTYLDLQGISIAADQGLDDLIVSL
                     AAGETDFKQLAETLRRLDKE"
BASE COUNT          866 a          839 c          792 g          938 t
ORIGIN      
        1 cgctgaaatt cgaccagttg atactcgaat ttcctgagcg cggggacggc gcatgggtac
       61 acatcggttt ccgacgcaac agcccgcaac gcaaccagat actgaccgca accaagaaaa
      121 acggcaaaac cgtgtatctg cccgggctgc atccttgagg tcgtctgaaa tggatatttt
      181 attgaaatac tggaagccgg taggtgtatt gctgctaatc gtcctgattt ttaccgcatg
      241 gcatttcgac cgtgccgaaa aataccgcat gggacgggag gctgctgctg ccgaaatctc
      301 gaatcgtctg aaagacggct atatcgagca ggcaaagcag gcgcgttctg ccgagcagaa
      361 ggccgctgcc gcgtttgccg aacgacaaac caaattagaa gaggaaaaac aaaatgctga
      421 aaaaactgtt gccgctatgc gtcttgagct taaccgcctg cgccactacg ccgccgccca
      481 aaatcgcaac agaaacctgc ccgcaaccgc taccgccgcc accgcatctg atggcgcgtc
      541 agattcccaa ggctggctat tattcggaca gtgcgctgaa aaatatgcag gactggcaga
      601 aacagccgac ggacatgcag ccgatttaag ggaatggcag gcatatggtc atgcagtaag
      661 cagtcagcgt gccgaataag caaccgcccg aacctgtaag aaaagattac aggttcgggc
      721 ggtttcagca tttaatcgaa taagacggcg ccgatgcgcg ccagcacatc gtccaataca
      781 taatcgggta cagtttcttt aaaagaagcc ttgcggattt gccagtctag agatttgagc
      841 tggtggcagt ggacattgcc ctgcgtttcc gttcctgcac cgagtaaggt tgaaatcatg
      901 ccgctgcttc gtgcagctgc tgcattcccc tgtgaaatgg ggcaggcaaa aaccaatccc
      961 gttgcgcggt tgaatgcttt tggagacaga gccagcgcaa accgcccgcc cttgatttcc
     1021 ttgccgctgg aagggtcgaa attcaaatgg aaaatatcgc ctttgtcggg aatatacatt
     1081 tcagacgacc tcgttgccgg catcatccaa gatttcccag ccttctacgc gcggcggggt
     1141 ttcttccatt tcggcaagca agtctgccaa gcggaaacgt cgggcagcac gcacacggag
     1201 ttcgccgtta tgtacttccg ctaccaaagc gtcgccgatt ttaaaatcca attgtttcag
     1261 catgtcggca ggcagtcgga cggccgccga gttcccccat ttttggacac gcaacataat
     1321 cttcaccttt attgtatcta caaagtagat acatattacc ataaaatttc agttgttcaa
     1381 atacttgtgc agaatacgcc aaaagccgtc cgaactgttt cggacggctt ttgtactgta
     1441 tttgcgcctt caggcaatat tttgttatcc attttcaaga tgcaaaagct ttctaattgc
     1501 ttgatgtcgg atttcggttg tttagggata caaaaccaag taaactaaaa ctgtttgatt
     1561 ggaaaatgct ccgcaaggag aaaattatgt tcaaaaaatc actttataag gctgctttgg
     1621 cgtatttcgg cgattgcgtg gctgcccata tttcagaaca gttttgactg tttattcaca
     1681 aatcagatgc ctttaggggg tttgctttcc ataatgcaac caaaatttcc aactctctaa
     1741 atattgtgtc tttgcgttct tcttcgcgta ttttcataac aagaggcgaa actgcattcc
     1801 acaattttat gaagttggtg caatgcagcc gtttaaacaa atcctcatcc aatactcccg
     1861 agttgattgc gcacgcataa aactcgtgcc gattgattac ggtaagtaaa tgccctctct
     1921 ctttttccca ttgtccgccc ttattttgat aaatttcata caggtcgatc cgcgcgttgt
     1981 tgtcgtcaac tgccgtgccg cgaatataag gcgaaatatg attgtcggct tctacaaatt
     2041 gtgcatcttg gtaatcattc aaaataacat ctaatgtcgc cttttgcttt gaagttttct
     2101 tattgatgaa tattgtcccc agtgcaatga cggcggttac tgaaacaacg gtcagttgcc
     2161 aaaacatcag ccattcggcc gtccctcata ggttaaactg tacgctgtgc ctgaatatgt
     2221 aaaataaagt gaatacagat acaaacaagg aggttagggc aattactgac aaccaacctc
     2281 cttgttttat ttgtttccgc accttaccag ccattaaaac cctcattttt gtatttcatc
     2341 gtatttcctt aatggtttta accttgcttg tcgatatctg tatattaatg ccgtgcgccc
     2401 gttctttcaa ggggtgattt aaaaatcagg catccttgac atcctctcct gcttaaaggc
     2461 ggggggactc ctgccgtgta aaccaatgcc gtctgaaggg ctttcagacg gcataaaaaa
     2521 accgcctttg tgtaaagcgg ttgaagaaaa gcctttcaat aaaaatgccg tctgaatttc
     2581 agacggcatt gttgtcggat atgcctattc cttatccagc cggcgcaggg tttcggcgag
     2641 ctgtttgaag tcggtttctc ccgccgccaa actgacaatc aagtcgtcaa gcccttggtc
     2701 ggcggcaatg ctgatgccct gcaaatccag ataggtcaac attgttaaaa gcgcggtgcg
     2761 cttgttgccg tcgggaaagg cgtgggcttt ggctatggct tgtgcataga gggcggcgat
     2821 ttcgtagatg tcctcaaggt tttcatactg ccgccagttg gcaatccgcg acaatgcgcc
     2881 gtccaagcgc gccatatccg cccgcccttt caaacccgct tcatccgcca atacggtttg
     2941 atggataagc gcgaccagtt cgccgtctat catttgtcgg caagtgcctt gacggctttt
     3001 tgatgggttt tggcaatgcg gcgggcggcg gcaagcagta cgcgtttgcc tgcttcgcct
     3061 ttaagctcga ttttgacggg gcgtgtattt tggttttgca tcgctgctcc ttgtctgttt
     3121 gcgggcattt tagctttttt ccggcagctt gggaaatgcc gtccgaaaac acttcagacg
     3181 gcattcttct aatagtgtaa tgcaatagtt actccaagat ttttgtatat ggctgttttt
     3241 atggtaaatt aaattttcat aagaaataaa tatatttaaa tcaatgaaat aaaatttagt
     3301 cgaatcccac cgtttccgcc acaaagcaaa accgccctga ttcggggcgg ttcttttttg
     3361 ttcaagttga gacctttgca aaattccttt ccctcccgac agccgaaacc caaacacagg
     3421 ttttcagctg ttttc
//
LOCUS       DAWGRZ010000187         3434 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000187 DAWGRZ010000000
VERSION     DAWGRZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.187"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     regulatory      65..168
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            235..1458
                     /gene="cytX"
                     /locus_tag="WHT44_001858"
     CDS             235..1458
                     /gene="cytX"
                     /locus_tag="WHT44_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ2533995.1"
                     /translation="MSGNASSPSSSSAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            1455..2555
                     /locus_tag="WHT44_001859"
     CDS             1455..2555
                     /locus_tag="WHT44_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ2533996.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECVPEGLQAQYDWLIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAIHPAFGEADILEIATGLRPTLNHHNPEIRYNRARRLIEINGLFRHGFMISPAVT
                     AAAARLAVALFDGKDAPERDKESGLAYIRRQD"
     gene            2577..3194
                     /gene="thiE"
                     /locus_tag="WHT44_001860"
     CDS             2577..3194
                     /gene="thiE"
                     /locus_tag="WHT44_001860"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ2533997.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKALHGDE
                     LKREIARCAAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYIASGAIFPTTTKQMPTAPQGLDKLREYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT          695 a         1080 c          950 g          709 t
ORIGIN      
        1 aatgcgccgt gccgcctgaa atgtaagatt tttgccaacg ccccctgctt ttgtgtacac
       61 ttaaagctcc ttgtcggagt gccgccgccg ggcggctgag attgcgaaag cagaatccgt
      121 agaacctgtc ggggtaatgc ctgcgtagga aacaaaccgt caaatgcctt atcaggcttc
      181 cgttcccttt tccgcacttc cccgccccat tttcatgttt tttaaggact tgatatgtcg
      241 ggcaatgcct cctctccttc atcttcctcc gccatcgggc tgatttggtt cggcgcggcg
      301 gtatcgattg ccgaaatcag cacgggtacg ctgcttgcgc ctttgggctg gcagcgcggt
      361 ctggcggctc tacttttggg tcatgccgtc ggcggcgcgc tgttttttgc ggcggcgtat
      421 atcggcgcac tgaccggacg cagctcgatg gaaagcgtgc gcctgtcgtt cggcaaacgc
      481 ggttcagtgc tgttttccgt ggcgaatatg ctgcaactgg ccggctggac ggcggtgatg
      541 atttacgccg gcgcaacggt cagctccgct ttgggcaaag tgttgtggga cggcgaatct
      601 tttgtctggt gggcattggc aaacggcgcg ctgattgtgc tgtggctggt tttcggcgca
      661 cgcaaaacag gcgggctgaa aaccgtttcg atgctgctga tgctgttggc ggttctgtgg
      721 ctgagtgccg aagtcttttc cacggcaggc agcaccgccg cacaggtttc agacggcatg
      781 agtttcggaa cggcagtcga gctgtccgcc gtgatgccgc tttcctggct gccgcttgcc
      841 gccgactaca cgcgccacgc gcgccgcccg tttgcggcaa ccctgacggc aacgctcgcc
      901 tacacgctga ccggctgctg gatgtatgcc ttgggtttgg cagcggcgtt gttcaccgga
      961 gaaaccgacg tggcaaaaat cctgctgggc gcaggtttgg gtgcggcagg cattttggcg
     1021 gtcgtcctct ccaccgttac cacaacgttt ctcgatgcct attccgccgg cgcgagtgcg
     1081 aacaacattt ccgcgcgttt tgcggaaaca cccgtcgctg tcggcgttac cctgatcggc
     1141 acggtacttg ccgtcatgct gcccgttacc gaatatgaaa acttcctgct gcttatcggc
     1201 tcggtatttg cgccgatggc ggcggttttg attgccgact ttttcgtctt gaaacggcgt
     1261 gaggagattg aaggctttga ctttgccgga ctggttctgt ggcttgcggg cttcatcctc
     1321 taccgcttcc tgctctcgtc cggctgggaa agcagcatcg gtctgaccgc ccccgtaatg
     1381 tctgccgttg ccattgccac cgtatcggta cgccttttct ttaaaaaaac ccaatcttta
     1441 caaaggaacc cgtcatgacc cgtatcgcca tcctcggcgg cggcctctcg ggaaggctga
     1501 ccgcgttgca gcttgcagaa caaggttatc agattgcact tttcgataaa ggctgccgcc
     1561 ggggcgaaca cgccgccgcc tatgttgccg ccgccatgct cgcgcctgcg gcggaagcgg
     1621 tcgaagccac gcccgaagtg gtcaggctgg gcaggcagag catcccgctt tggcgcggca
     1681 tccgatgccg tctgaacacg cacacgatga tgcaggaaaa cggcagcctg attgtgtggc
     1741 acgggcagga caagccatta tccagcgagt tcgtccgcca tctcaaacgc ggcggcgtag
     1801 cggatgacga aatcgtccgt tggcgcgccg acgacatcgc cgaacgcgaa ccgcaactcg
     1861 gcggacgttt ttcagacggc atctacctgc cgaccgaagg ccagctcgac gggcggcaaa
     1921 tattgtctgc acttgccgac gctttggacg aactgaacgt cccctgccat tgggaacacg
     1981 aatgcgtccc cgaaggcctg caagcccaat acgactggct gatcgactgc cgcggctacg
     2041 gcgcaaaaac cgcgtggaac caatcccccg agcacaccag caccctgcgc ggcatacgcg
     2101 gcgaagtggc gcgggtttac acacccgaaa tcacgctcaa ccgccccgtg cgtctgctcc
     2161 atccgcgtta tccgctctac atcgccccga aagaaaacca cgtcttcgtc atcggcgcga
     2221 cccaaatcga aagcgaaagc caagcccccg ccagcgtgcg ttcagggttg gaactcttgt
     2281 ccgcactcta tgccatccac cccgccttcg gcgaagccga catcctcgaa atcgccaccg
     2341 gcctgcgccc cacgctcaac caccacaacc ccgaaatccg ttacaaccgc gcccgacgcc
     2401 tgattgaaat caacggcctt ttccgccacg gtttcatgat ctcccccgcc gtaaccgccg
     2461 ccgccgccag attggcagtg gcactgtttg acggaaaaga cgcgcccgaa cgcgataaag
     2521 aaagcggttt ggcgtatatc cgaagacaag attaaagccg cccgaaagga caccttatga
     2581 ccttcccgcc cctaaaatcc ccgctcaaat tctacgccgt cgtccccacc gccgattggg
     2641 tggggcgcat ggtcaaagca ggtgccgaca cggtgcaact gcgctgcaag gccctgcacg
     2701 gcgatgaatt gaaacgcgaa atcgcccgct gcgccgcagc ctgtcagggc agccgtacgc
     2761 agcttttcat caacgaccac tggcgcgaag caatcgaagc gggcgcgtac ggcgtgcatc
     2821 tcggacaaga agacatggac accgccgacc ttgccgccat cgccgccgcc ggtttgcgct
     2881 tgggtttgag tacgcactcc gttgccgaac tcgaccgcgc cctgtccgta caccctagct
     2941 acatcgccag cggcgcgatt ttcccgacca cgaccaaaca aatgcccacc gccccgcaag
     3001 gcttggacaa actgcgcgaa tacgtcaaac aagcaggcgg cacgcccgtc gtcgccatcg
     3061 gcggtatcga cttgaacaac gcccgagccg tactcgccac cggcgtttcc tcactcgccg
     3121 ccgtccgcgc cgtaaccgaa gcggcaaatc ccgaagcggt ggttaaagcg tttcaggctt
     3181 tgtgggatgg ataaaaccga aagaagaaaa ttcaattgcc gtgtaggcaa aacttagccc
     3241 gttatcgcaa acatacttaa ctttaaatgt ggcatatcat caaattccgt cattcccgcg
     3301 taagcgggaa tccgccttaa aacttgagaa accatcattt gaaaaacagt ttccgaattt
     3361 caaaaatgga ttcccgcccg tgcgggaatg acggcaaccg gtcagttgcg tatcaaaaaa
     3421 taaagtaatt cggc
//
LOCUS       DAWGRZ010000188         3406 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000188 DAWGRZ010000000
VERSION     DAWGRZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.188"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            43..711
                     /locus_tag="WHT44_001861"
     CDS             43..711
                     /locus_tag="WHT44_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ2533998.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGTPNNKCDSGKKGQGMLAFGDKNGNK
                     GYDNDTEDVLLRSVVLNDDINDKRINYAFNHIAFGQTQPTTDRVVWTFNQNGTFGYTK
                     DQHLTKQSSFFYSDGYIQIVLTDAKAVSADEKKFRSAVVLINSSGRVEVCPRGDRRTM
                     CQYK"
     gene            735..1343
                     /gene="pilV"
                     /locus_tag="WHT44_001862"
     CDS             735..1343
                     /gene="pilV"
                     /locus_tag="WHT44_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ2533999.1"
                     /translation="MKNNDCFRLKDSQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNHTLSAVDGDFAIDAM
                     KTKGQLAEAQLKRFSYELKNALPDAAAIHYAVCKDSSGNAPTLSGNAFSSNCDNKANG
                     DTLIKVLWVNDSAGDSDISRTNLEVSGDNIVYTYQARVGGRE"
     gene            1340..2281
                     /locus_tag="WHT44_001863"
     CDS             1340..2281
                     /locus_tag="WHT44_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ2534000.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGLLSMIVLMAVGSSYFT
                     SRKLNDAANERLAAQQDLRNAATLIVRDARMAGGFGCFNMSEHPATDVIPDTTQQNSP
                     FSLKRNGIDKLIPIAESSNINYQNFFQVGSALIFQYGIDDVNASTATTVVSSCAAISK
                     PGKQIPTLEDAKKELKIPDQDKEQNGNIARQRHVVNAYAVGRIADEEGLFRFQLDDKG
                     KWGNPQLLVKKVRHMKVRYIYVSGCPEDDDAGKEETFKYTDKFDSAQNAVTPAGVEVL
                     LSSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2260..2853
                     /locus_tag="WHT44_001864"
     CDS             2260..2853
                     /locus_tag="WHT44_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ2534001.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCAAVNVRT
                     NNDNEEAFDNIVVQGKPTVEAVKRSCPANSTDLCIDKKGMEYKKGTRSVSKMPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2858..3331
                     /locus_tag="WHT44_001865"
     CDS             2858..3331
                     /locus_tag="WHT44_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ2534002.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQTIENKLEIFVSGYKMNPKIAKKYSVSVKFVDKEKSRA
                     YRLVGVPKAGTGYTLSVWMNSVGDGYKCRDAASAQAHLETLSSDVGCEAFSNRKK"
BASE COUNT          976 a          675 c          863 g          892 t
ORIGIN      
        1 aatttaatcc acgatacatt accagttaac gttctattgc ttatgtgtac acgaaaacaa
       61 caaggtttca cgctaacaga gctgctcatc gtgatggtca ttgcagccat tatggcgatg
      121 atagccctcc ccaatatgag ccaatggatt gcatcccgcc gcattgccag tcacgcggag
      181 cggattgcca accttttgcg tttctccagg ggcgaagccg tccggctcaa tctccctgtc
      241 tatatctgtc ctgttcaagt taaaaaagac ggtacgccca acaataaatg tgactccggc
      301 aagaaggggc agggaatgtt ggctttcggc gacaaaaacg gcaataaggg atatgacaat
      361 gatacggagg atgttcttct ccgcagtgtg gtattgaatg atgatatcaa tgataagcgg
      421 attaattatg ccttcaacca tatcgctttc ggtcagactc agccgaccac cgaccgtgta
      481 gtttggacat tcaatcaaaa cgggacgttc ggttatacga aagaccagca tcttacaaaa
      541 caatccagct ttttttattc tgacggttat atccaaatcg tgttgacaga tgcgaaggcg
      601 gtttctgccg atgaaaagaa attccgttcg gcggtggttt tgattaacag cagcggcagg
      661 gtcgaagttt gccctagggg tgatcggcgt actatgtgcc agtataaata acagtttcag
      721 ttttaaaaat gaatatgaag aataatgatt gcttccgcct gaaagattcc cagtccggta
      781 tggcgctgat agaagtcttg gttgctatgc tcgttctgac catcggtatt ttggcactat
      841 tgtctgtaca gttgcggaca gtcgcttccg tcagggaggc ggagacacaa accatcgtca
      901 gccaaatcac gcaaaacctg atggagggaa tgttgatgaa tccgaccatt gattcggaca
      961 gcaacaagaa aaactataat ctttacatgg gaaaccatac actatcagct gtggatggcg
     1021 attttgcgat tgatgccatg aaaactaagg ggcaattggc agaggcacaa ttgaagagat
     1081 ttagttatga gctgaaaaat gccttgccgg atgcggcagc catccattac gccgtctgca
     1141 aggattcgtc gggtaacgcg ccgacattgt ccggcaatgc tttttcttca aattgcgaca
     1201 ataaggcaaa cggggatact ttaattaaag tattgtgggt aaatgattcg gcaggggatt
     1261 cggatatttc ccgtacgaat cttgaggtga gcggcgacaa tatcgtatat acttatcagg
     1321 caagggtcgg aggtcgggaa tgagacgtaa aatgctaaac gtaccaaaag gcagttatga
     1381 tggtatgaaa ggttttacca ttattgaatt tttggttgcg ggcctgctca gtatgattgt
     1441 cctgatggcg gtcggatcga gttacttcac atcccggaaa ttaaatgatg cggcaaacga
     1501 gcgtcttgcc gcgcaacagg atttgcggaa tgcggcaaca ttgattgtcc gcgatgcgag
     1561 aatggcaggc ggcttcggtt gtttcaatat gtccgagcat cctgcaactg atgttattcc
     1621 cgatacgacg caacaaaatt ctcctttttc cttaaaaagg aacggtatag ataaacttat
     1681 tcccatagcg gaatcttcaa atatcaatta tcagaatttt ttccaggttg gtagcgcatt
     1741 gatttttcaa tacggaatcg atgatgttaa tgcaagcacc gcgactaccg tcgtcagcag
     1801 ctgtgccgca atatcgaaac cgggcaagca aatccctact ttagaagatg caaaaaaaga
     1861 attgaagatt ccggatcagg ataaggagca aaatggcaat atagcgcgtc aaaggcatgt
     1921 ggtcaatgcc tatgcggtcg gcaggattgc cgatgaggaa ggtttgttcc gcttccaatt
     1981 ggatgataag ggcaagtggg gtaatcctca gttgctcgtg aaaaaggtta gacatatgaa
     2041 agtgcggtat atctatgttt ccggctgtcc tgaagatgac gatgccggca aagaggaaac
     2101 attcaaatat acggataaat tcgacagcgc ccaaaatgct gttacgcccg ccggggtgga
     2161 ggttttattg agtagcggta ctgataccaa gattgccgct tcttcagaca atcatattta
     2221 tgcttaccgt atcgatgcga caatacgcgg gggaaatgta tgcgcaaaca gaacactttg
     2281 acgggaatcc cgacttctga cggacagagg gggtttgcac tgtttatcgt gctgatggtg
     2341 atgatcgtcg tggctttttt ggttgtaact gccgcgcagt cttacaatac cgagcagcgg
     2401 atcagtgcca acgaatcaga caggaaattg gctttgtctt tggccgaggc ggctttgcgg
     2461 gaaggcgaac ttcaggtttt ggatttggaa tatgatacgg acagtaaggt tacatttagc
     2521 gaaaactgtg gaaaaggtct gtgtgccgca gtgaatgtgc ggacaaataa tgataatgaa
     2581 gaggcttttg acaatatcgt ggtgcaaggc aagcccaccg ttgaggcggt gaagcgttct
     2641 tgccctgcaa attctaccga cctgtgcatt gacaagaaag ggatggaata taagaaaggc
     2701 acgagaagcg tcagcaaaat gccacgttat attatcgaat atttgggcgt gaagaacgga
     2761 gaaaatgttt atcgggttac tgccaaggct tggggtaaga atgccaatac cgtggtcgtc
     2821 cttcaatctt atgtaagcaa taatgatgag taataaaatg gaacaaaaag ggtttacatt
     2881 gattgagatg atgatagtcg tcgcgatact cggcattatc agcgtcattg ccataccttc
     2941 ttatcaaagt tatattgaaa aaggctatca gtcccagctt tatacggaga tggtcggtat
     3001 caacaatatt tccaaacagt ttattttgaa aaatcccctg gacgataatc agactatcga
     3061 gaacaaactg gaaatatttg tctcaggcta taagatgaat ccgaaaattg ccaaaaaata
     3121 tagtgtttcg gtaaagtttg tcgataagga aaaatcaagg gcatacaggt tggtcggcgt
     3181 tccgaaggcg gggacgggtt atactttgtc ggtatggatg aacagcgtgg gcgacggata
     3241 caaatgccgt gatgccgctt ctgcccaagc ccatttggag accttgtcct cagatgtcgg
     3301 ctgtgaagcc ttctctaatc gtaaaaaata aggttgtttt gccaataccg tctgaaaatc
     3361 aatgttcaga cggtattttt atgggtatag tggattaaca aaaatc
//
LOCUS       DAWGRZ010000189         3354 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000189 DAWGRZ010000000
VERSION     DAWGRZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.189"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            142..1285
                     /locus_tag="WHT44_001866"
                     /pseudo
     CDS             142..1285
                     /locus_tag="WHT44_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246189.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(1395..1604)
                     /locus_tag="WHT44_001867"
     CDS             complement(1395..1604)
                     /locus_tag="WHT44_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534003.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPAYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(1601..1897)
                     /locus_tag="WHT44_001868"
     CDS             complement(1601..1897)
                     /locus_tag="WHT44_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ2534004.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRRARL
                     AQKPKTRWKILYTLINGVFFDRHHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            complement(1876..2481)
                     /locus_tag="WHT44_001869"
     CDS             complement(1876..2481)
                     /locus_tag="WHT44_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534005.1"
                     /translation="MTIYFKNGFYDDTLGGIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSDYHEWDGKKWKISKAAAAARFAKQKTALAFRLAEKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVIEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            2561..2848
                     /locus_tag="WHT44_001870"
     CDS             2561..2848
                     /locus_tag="WHT44_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ2534006.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVRFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            2820..3122
                     /locus_tag="WHT44_001871"
     CDS             2820..3122
                     /locus_tag="WHT44_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ2534007.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYIAQLK"
BASE COUNT          727 a          898 c          885 g          844 t
ORIGIN      
        1 gcaacgctgt actggtttaa atttaatcca ctataaatta acacgcaatg taccctgacc
       61 ggcaaaactt ccccaaactt catcaaaaaa tcaactgtac ccgtcttttt cagacggcat
      121 tgataccgta agaaatttcc catgcagaaa tggcaaaccg agctttattc cacaccgctt
      181 tggctttgtc tgacaccgaa aagcgcggtc aaagtcttgc tgctgattac ggcgatgatt
      241 acgctggtgt taaccgaaat gcggctgaat gtgttgagta cctttatgtc caaagggctt
      301 tacgactcga tgcaggattt gaaatgcttc ggcattttgg atgtttgctg caatgaacgc
      361 gggcgtggtg ctgatacggg cgtttaacaa cgtcgtcaac gacttcctcg atcaaggctt
      421 ggcgattaaa tggtcggagc ggctcaacga agtgctgaca gcacgctggc ttgccgacaa
      481 aaactactac cgcctgcaaa tgcgccgcca cgcgccggac aacatcgacc agcgtatcca
      541 acaggacgcg caggaattta tcgcttcgac catcgaattt gtgcgcggca tggtcaattc
      601 ggtcgttact tctctggaat ttgccgttgt tttgcggggc ttggctggca tcctgaccgt
      661 gttcggcttc gacattccgc acggcatcgt ttggtttgtc tatatatttg tgattttggc
      721 gacctttatc gccatgtgga taggcaaccc tttgattcgt tacaattatg aaaacgaaaa
      781 actcaacggc gactaccgtt attccctcat ccgcgtgcgc gaccacgccg aaagcgtggc
      841 gttttacagc ggcgaaaaac acgaacacga ccagctttct gaccgtttta aagccattat
      901 ccgcaaccgc tggcgtattg cgcgacaaag cgtctgcctg agcggcttta atgatatgtt
      961 caccaacggc atcaagctct tccccattat tttgcaagcg ccgcgcctgt ttgccggaca
     1021 aatcaaaatc ggcgacatcc agcagaccgt ccaagccttc gcgcgactgc aaaacgcgct
     1081 ttctttcttc cggatgttct acaacaaatt caccgcctac cgcgcccgat tggagcgtct
     1141 gtacggcttt ttgctgagta ccgaagaaca acacagcgcg cggcaacccg agattagcga
     1201 agtttcagac ggcattgcac tggaatgtaa ggcggcggca gcttttgcac gaattggcgg
     1261 cagcttttgc accgccgaag cgtaggaaat tgtattagct tttgcacaaa tttatccccg
     1321 tttaatggtc tgttaaacgg ggattatctt atgcctttaa aagtagtacg ctacttgcgg
     1381 tcagaactta taaatcaggt ggtcgtcgta gcttgaattg ctgttgatgt gctgctcaca
     1441 gtcaaggttt ggctgcctgc aaaacgctcc catccggtca ggcgcagttc tgccacgagg
     1501 tcaagataag caggcagttc gctgcgggtg ccggagaaga agacaaatgg cggccgcacc
     1561 attgccatca ggcgcagaaa ctctaccatg ccgaagtagt tcatcgcgtc cccttttccc
     1621 cggcggcgcg gctttggttg aaccgcgcgt cgtgctgcct gcctttcagt tcgctgatat
     1681 acgcctgccg gcagtggtgg cggtcgaaaa acacgccgtt gatcagggta tataaaatct
     1741 tccagcgggt tttgggcttt tgggcgagcc gcgcgcggcg ataggtgcgg cttgagaggg
     1801 tttcgtccgc cgcgccgccg gttagggcgt tgaacagttg gtcgatggct accaacaggt
     1861 ggtaaacgta ttttttcagc cgatgtttag cgtccattct tcgatttcct tttccagcgc
     1921 gtccaatccg ggcgcggttt cgatggtgtt caatttgtct tcgagctgct gacgctttcc
     1981 gataatcgcg ccggcggcaa cagccaggcg ggcggatttt tcgataactt tttcaatcaa
     2041 aacgtccaat tccacgcccc ttgcggcggc gatttgcgcc agcatcgggg tcggggcgtt
     2101 gttgtccgcc tgccgcgcga gggcttcttt ttcctgcctg taaaagctgt cgatttccac
     2161 ttggggatag cccgccaaga ggctgttttt gagttcgtcc gccttttccg cgaggcggaa
     2221 tgccaaggcg gttttttgtt tggcgaaacg ggcggcggcg gcggctttgc tgattttcca
     2281 ttttttgccg tcccattcgt ggtaatcgga cgggcgcggc ggggttaaaa cggggcggcc
     2341 gtcggaatct gcggcaatct gcccgccctg cgcctgtcct gccaaaaggg cggcgtattc
     2401 ttcggcgcgg acggcaaccg cgccttcggg gatgccgccc aatgtgtcgt cgtaaaagcc
     2461 gtttttgaaa tagatggtca ttttggggtt tccttttttg tatttacatt ttggtttttt
     2521 gccgcttata atgtaacgac aaaatacgct gcaagctgca atgaagattg aatttgactc
     2581 cgaaaaaaat caacgcaata tcgaagagcg gaatttgccc tttgaatcag tcgggcaaat
     2641 acgctggaca acggccgtta ttgtgcccga tgtccgcttt gattaccccg aaccgcgtta
     2701 tgttgccgct gcttatctag gcgatacgca acgcctgcac attgtctgtt ttacgccgat
     2761 aaaagacggc atccgtgtca tcagttttcg caaagcgaat aaaagagagg ttaaaaaata
     2821 tgcaacgtca atccttaaca aacgctgacg gcgaagtgcg ggaacttgcc gccgaagatt
     2881 ttgcgcttgc ccgccccatt gccgaagccc tgcccgaaga cttggcacag gtgctttttt
     2941 cccatcaaaa acaattggaa gaaaagggcg taatgcaaaa acgaaacaca ggcaaaaccc
     3001 cgaaacaact cgttaccata cgcctgtccg ccgatgtggt cgaaaaattc cgcgccggcg
     3061 gcaagggctg gcagacgcgc atcaacgagg ttttgcgcca atatatcgcc caattgaaat
     3121 aacgggggcg gccgctgctt atcgggcgcg cccgactatc ctttcccgcg cggtttcggc
     3181 cgtgcggttt tttgttgccg gtgccgtccg aagggggcag cggctttttg cgcggtcatt
     3241 cccgcctttc gttattcccg ccactttccg tcattcccgc gaaggcggga atcccgaaac
     3301 ccctgccgcc gtcattcccg cgcaggcggg aatccagtcc gttcggtttc agcc
//
LOCUS       DAWGRZ010000190         3294 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000190 DAWGRZ010000000
VERSION     DAWGRZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.190"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(184..405)
                     /locus_tag="WHT44_001872"
     CDS             complement(184..405)
                     /locus_tag="WHT44_001872"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534008.1"
                     /translation="MLSTRKTARKIQARHIALPFSGKPQFYRPQERLSKQAQATPPGH
                     FRFHRLSVVRIMQQHHQRITLFGRQQAEI"
     gene            464..1087
                     /locus_tag="WHT44_001873"
     CDS             464..1087
                     /locus_tag="WHT44_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ2534009.1"
                     /translation="MRKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1198..2580
                     /locus_tag="WHT44_001874"
     CDS             1198..2580
                     /locus_tag="WHT44_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ2534010.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYAGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNQEGLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2584..3108
                     /locus_tag="WHT44_001875"
     CDS             2584..3108
                     /locus_tag="WHT44_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ2534011.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          682 a          863 c          994 g          755 t
ORIGIN      
        1 aggattaaag gggtatttgg gtaaaattag gaggtatctg gggcgaaaac agccgaaaac
       61 ctgtgtttgg gtttcggctg tcgggaggga aaggaatttt gcaaaggcct caagatatag
      121 tggattaaat ttaaatcagt acggcaaggc gaggcaacgc tgtactggtt taaatttaat
      181 ctactatatt tccgcctgct gccgcccgaa aagcgtgatg cgctgatggt gctgctgcat
      241 aatccggacg acggataacc ggtgaaaacg gaaatgcccg ggcggtgttg cctgtgcctg
      301 tttggacaag cgttcttgag ggcggtagaa ttgaggtttg cccgaaaagg gcagggcgat
      361 atgccgcgct tggattttgc gcgcggtttt tcgcgttgaa agcaattatg tctgtttgaa
      421 tatttaatgc cgtctgaaac gtccggacgc gcccccgttc cctatgagaa aaccgaccga
      481 taccctaccc gttaatctgc aacgccgccg cctgttgtgt gccgccggtg cgttgttgct
      541 cagtcctctg gcgcacgccg gcgcgcaacg tgaggaaacg cttgccgacg atgtggcttc
      601 cgtgatgagg agttctgtcg gcagcgtcaa tccgccgagg ctggtgtttg acaatccgaa
      661 agagggcgag cgttggttgt ctgccatgtc ggcacgtttg gcaaggttcg tccccgagga
      721 ggaggagcgg cgcaggctgc tggtcaatat ccagtacgaa agcagccggg ccggtttgga
      781 tacgcagatt gtgttggggc tgattgaggt ggaaagcgcg ttccgccagt atgcaatcag
      841 cggtgtcggc gcgcgcggcc tgatgcaggt tatgccgttt tggaaaaact acatcggcaa
      901 accggcgcac aacctgttcg acatccgcac caacctgcgt tacggctgta ccatcctgcg
      961 ccattaccgg aatcttgaaa aaggcaacat cgtccgcgcg cttgcccgct ttaacggcag
     1021 cttgggcagc aataaatatc cgaacgccgt tttgggcgcg tggcgcaacc gctggcagtg
     1081 gcgttgattt tgaacccgcg ccgcaaccga aatacggcga atcctgtata atccgaaaat
     1141 ctgttcactg gaagttcaga cggcattgca actgttgatg ccgtctgaaa aaatatgatg
     1201 gcaagagaca accgcatcca aatgtttccg cacgaatggc gcgccagtac gacgctttcc
     1261 ggcgtgtacg cgctgcgtat gctgggtatg ttcctcgtgc tgcccgtatt ggcggtgtat
     1321 gccgcctcgc tgcccggcgc ggaaggcaac aaaacgctgg tcgggctggc aatgggcatt
     1381 tacgggctga cacaggctct gctgcaactg cctttgggca tcgcttccga caagttcggg
     1441 cgcaagaaaa ccatttatgc gggactggtc gtgtttgcgg cgggcagctt tcttgccgcc
     1501 gccgccgata cgctgcccat gctggtcgca gcacgcgcca tacagggtgc aggggcggtc
     1561 agtgcggcgg ttaccgcgct gctggcggat ttgacgcgcg acggcgtacg tacccgcgca
     1621 atggcgatga tcggtttgag tatcggtttg acgttttcgg tcagcctcgt cgttgccccc
     1681 gtgattgccg acgcggtcgg cgttcgcgga ctgtttatgc tgaccggcat tctgaccgtc
     1741 atcagcatcg gcgtggtggc gtggatgact cccgatcccg aagtttccaa gctgcacgaa
     1801 gatacgcagg cgcagccttc gcgcataggc gaagttttga aaaaccgtag gctgctgacg
     1861 cttgatttcg gcattttcgc cctgcacgcc gcacaaatgg cattgtttac cgcgctgcct
     1921 ttcgcgatga cccagctcgg tttggaaaaa atacagcatt ggaaagtcta tctgccttcg
     1981 accattacgg gcttggtggt gatggttccg ctgattatcg tcggcgagac gcgcaacaag
     2041 cttaagcagg tttttgtttt gggtatcgtc tgtattgcgg cggcgcagct cggtttgctg
     2101 tccggtatgc gctcggtagg cttgattacc gcttatttgg ttgtttactt tatcggtttt
     2161 aatgtgttgg aagcgagcct gccgtctatg gtttccaaaa tcgcgccgtc cgacctgaag
     2221 ggtacggcga tgggcgtgta caacacgatg cagtcgctcg gactgtttgc cggcggcgcg
     2281 gcaggcggtt tgctgtttca aaaatacggc ttttccggcg tgtttgcctt ttgcagtata
     2341 ttgatgctgc tgtggctggt aattgccgtt ttatcgcctg cgcccaagcc cgtcaaaaac
     2401 ctcagttacc ctgtcggcgg cgtgtggcag ggcaatcagg aagggttata ccgcgccttg
     2461 tcggagcttg agggtgtgga agacatcggt ttcagtttcg acgggcagac cgtctatctc
     2521 aaagtgttgc agaagggttt cgatcaggct gccgctgaaa aaatcatcac aggagtttaa
     2581 aaaatgtcat tgaacaaagt catcctcatc ggccgcctcg gacgcgatcc cgaagtgcgc
     2641 tatatgccca acggcgaggc ggtttgtaat ttcagcgtcg ccaccagcga aacttggaac
     2701 gaccgcaacg gccaacgtgt agagcgtact gagtggcaca acatcaccat gtaccgcaaa
     2761 ctggcggaaa ttgccgggca atacctcaaa aaaggcgggc tggtttattt ggaaggcaga
     2821 atccaaagcc gcaaatacca aggcaaagac ggcatcgaac gcaccgctta cgatattgtc
     2881 gccaacgaaa tgaaaatgtt gggcgggcgc aatgaaaaca gcggcggtgc gccttacgag
     2941 gaaggttacg gtcagagtca ggaggcttac caacgccccg cgcagcaaag ccggcagccc
     3001 gcctccgacg cgccgtccca tccccaagaa gcaccagccg cgccgcgccg ccaacccgtg
     3061 cctgccgccg ccccggtcga ggacattgac gacgatatcc cgttctgaat tttacggccg
     3121 gacatcctcg cggagggaaa tcataaagga cggagaaacc ttaaacctta cggggcaggg
     3181 tttctctttt ttgcatttgg gccggcttgt tgccgccctt tgcgttgggc ggaacgttgc
     3241 gattgcgccg ttcgggcgtt ttgaggcagt gatgcctgcc gcacgtcccc gctg
//
LOCUS       DAWGRZ010000191         3292 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000191 DAWGRZ010000000
VERSION     DAWGRZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.191"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            83..1095
                     /locus_tag="WHT44_001876"
                     /pseudo
     CDS             83..1095
                     /locus_tag="WHT44_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            1311..2648
                     /gene="ribB"
                     /locus_tag="WHT44_001877"
     CDS             1311..2648
                     /gene="ribB"
                     /locus_tag="WHT44_001877"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ2534012.1"
                     /translation="MTDTAGLRRHNLRQWIEKYYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPSEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPAADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALEHIQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(2808..2945)
                     /locus_tag="WHT44_001878"
     CDS             complement(2808..2945)
                     /locus_tag="WHT44_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ2534013.1"
                     /translation="MKPVKLKMPSESLSDGILFGLENTYAVLSLKERIQKTKATGIYR
                     K"
     gene            complement(2956..3186)
                     /locus_tag="WHT44_001879"
     CDS             complement(2956..3186)
                     /locus_tag="WHT44_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ2534014.1"
                     /translation="MEDFSVVIGYDEIKAKNHSLSAGQYFEVKIDYVDISADEFAQKM
                     AGFSADLDKLFAESAELEKEIKDRLAMLKFNS"
BASE COUNT          860 a          901 c          734 g          797 t
ORIGIN      
        1 ttacagggtg cggatgcaaa cgcattgcga gcgcgggttt gaggcatacg cgcaaacatc
       61 ttaatataat ggattgatat ttatgatttt ctccatcatc gtccctattt acaatgtgga
      121 aaatacctcc gctgctgcgt ggattccgtg cttgccgaaa attttgccga ttatgaaatg
      181 attttggtcg atgacggttc gccggacggc tgcgggaaga tttgcgacga atatgcaggc
      241 aaatatccgc atataaaagt gattcatcaa gaaaacggcg ggctgtcgga tgcccgcaac
      301 gccggtatcc gggcggcaaa aggcgattac ctaatctttt tggacagcga cgattattgg
      361 gccgatacca accgttcaaa aaacgcgggg ggggggattc tctttgattt acaacaactt
      421 gcagacaaaa aggttgattt gatcctgcat ccctcgtcct tcaattaccg cgacatcccc
      481 aaaggggcgg acttttcgga taatgatttt gtccgccatt ttgaaacgct ggtggagggg
      541 cggtactata tcgccaacgc gtggacaaag attgtcaggc gggaaatcat cattaaaaac
      601 aatctgtttt tcccaaaagg atacattcac gaggatttcc cgtacagttt gcaattggcg
      661 cgttttatca agacttttgc cttttacgat aacccttttt accagtaccg cgttctcggc
      721 ggctctatca gccacaacat caaatacaaa aatttcagcg atgtgctgac gcatctcgac
      781 tggggtgtgg attttttagt cgaaaacaaa aattccccca tctacggcgg tttgcaaaaa
      841 tttgtcttcg acaatatcgg ctatctgagg tctatattgg taaggcttta tttttccaaa
      901 aacattatcc tcatctaccg gaaatatttt tcatttaaag aaaaatgcag aaagatattc
      961 ggcgcgaagg caatccgtcc ggtttttatc gggaagaccg cattcatcat aggattgccg
     1021 atattgcgcc tgctcgtacc gcctatgctg tacccggcaa tcaaggccgt ttatcagaaa
     1081 tttttttcgg aataagcttc cggcaaaccc cgaatcggaa gcgggcggga agaaacagcc
     1141 gccccgccgg cggggattgc ggcaatgccg tctgaagcca cgaatccggc ttcagacggc
     1201 atctgtttac caaaaagcaa ataattcggt ttggcgaaaa aaacagattt gctttttggt
     1261 aaatacgcga ttacaatccg ctacatccga tttctacaaa ggatgaaacg atgaccgaca
     1321 cagccggtct gcgccgccac aacctgcggc agtggataga aaaatactac ggcggtttgc
     1381 aaactcgttt tgctgaagcc gttgccctca acacaggcga actctccgcc cttttgaaaa
     1441 acaaatcctt cggcgagaaa aaagcccgta aaatcgaaca ggcggcaaaa atgcccgcct
     1501 tttggctcga taccgaacac accgcccgcc cgtccgaaca cacaggaaaa cacaccatgt
     1561 cccatatctc ccccatcccc gaaatcctag ccgacatcaa agccggcaaa atggtcatca
     1621 tcaccgatgc cgaagaccga gaaaacgaag gcgacctgct gatggcggcg caattcgtca
     1681 cgcccgaagc catcaacttc atgatcaaac acgcgcgcgg cttggtctgc ctgccgatgg
     1741 acggcgaaat ggtcgaaaaa ctcgggctgc cgatgatgac ccaaaaaaac ggcgcgcaat
     1801 acggcaccaa ctttaccgtc tccatcgaag ccgcacacgg cattaccacc ggcatttccg
     1861 ccgccgaccg cgccctgact attcaaaccg ccgtttcccc gaccgctaaa cccgaagaca
     1921 tcgtccaacc cggtcatatc tttccgcttc gcgcccaaaa aggcggcgta ctcgtccgcg
     1981 ccggacacac cgaagccggc gtcgacctgg cgcaaatgaa cgggctgatt cctgcctccg
     2041 ttatttgcga aatcatcaac gacgacggca cgatggcgcg tatgcccgaa ctgatgaaat
     2101 tcgccgaaga acacaagctc aaaatcggca cgattgccga cctcatcgaa taccgcagcc
     2161 gtaccgaaag cctgcttgaa gacatgggca atgcgcctgt acaaaccccg tggggcgagt
     2221 tccaacaaca cgtttacgtc gacaaactct ccggcgaaac ccacctcgcc ctcgtcaaag
     2281 gcacgcccgc cgccgacacc gaaaccctcg tccgcgtcca cgaacccttc agtgtgatgg
     2341 acttcattca agccaacccg cgccattcat ggtcgctgcc caaagccctt gagcacatcc
     2401 aacaagccga aagcggcgtc gtcatcctct tacaccgcac cgaagacggc gcatccctgc
     2461 tcgaccgaac cctacccaaa ggcgcaaacc aagcctacaa atgggacagc aaaagctacg
     2521 gcatcggcgc acaaatcctc gccggcctca acgtcaaaaa actgcgcgtc ctcgggcagc
     2581 cctcatcttt caccggcctg accggcttcg gtttggaagt cgtcggcttt gaagaagcgg
     2641 aaaaataata tagtaaattc aaatacttta tatttgcttt atttattgca ttatttccgt
     2701 gcaaacgaaa acccggtctg ttgggttgga ttttgttttt tcaaatttcg ggtaacttct
     2761 aattcgtcat tcccgcgctg gcgggaatcc ggttcgtcgg gtttttgtca tttccgataa
     2821 attcctgtgg ctttggtttt ttggattctc tctttcaggg aaagaacggc ataagtattt
     2881 tccaaaccaa acaaaatgcc gtctgaaagg ctttcagacg gcattttaag tttgaccggt
     2941 ttcattcatc ggtatttatg aattgaattt caacatcgcc aatctatcct taatctcttt
     3001 ttccaattcg gcagattcgg cgaaaagttt atccaaatcc gctgaaaatc ccgccatttt
     3061 ttgcgcaaat tcgtcggcgg aaatatccac ataatcaatc tttacctcga aatactgccc
     3121 cgccgacaag ctgtgattct tcgctttgat ttcatcgtag ccgattacca cactgaaatc
     3181 ttccactgcc tgtttgtgcg tgaaggtatt gcagattttt ttgttcttct tcgcgggaaa
     3241 gtacggtttt tttgccgtct ttaatttttt cgcccaagcc cgatgcgtcg at
//
LOCUS       DAWGRZ010000192         3251 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000192 DAWGRZ010000000
VERSION     DAWGRZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.192"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(653..1090)
                     /gene="rnhA"
                     /locus_tag="WHT44_001880"
     CDS             complement(653..1090)
                     /gene="rnhA"
                     /locus_tag="WHT44_001880"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ2534015.1"
                     /translation="MNQTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTASKQPVKNDD
                     LWKELDALVGRHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            1204..2073
                     /gene="tehB"
                     /locus_tag="WHT44_001881"
     CDS             1204..2073
                     /gene="tehB"
                     /locus_tag="WHT44_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ2534016.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKVENLPEVLLSGYSSEEGEWVCL
                     NVLQGDVEVRAPDGSAEVWSADGGDCVFAPQQVFSVKPKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAARDTVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGSMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            complement(2123..2929)
                     /gene="thiD"
                     /locus_tag="WHT44_001882"
     CDS             complement(2123..2929)
                     /gene="thiD"
                     /locus_tag="WHT44_001882"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ2534017.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCSFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSTSGRCTDWLFTQNETLEFDSPRFPTAHTHGTGCT
                     FSACITAELAKGSDVCEAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            2990..3187
                     /locus_tag="WHT44_001883"
     CDS             2990..3187
                     /locus_tag="WHT44_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534018.1"
                     /translation="MPSENRSAGVCGALYAVLQDKAQGRAAGCIVATVFLPDGVKNTV
                     ATPTDNEKREQCRLNRVLRRA"
BASE COUNT          677 a          789 c          951 g          834 t
ORIGIN      
        1 tttaatttgt ctgtccgcat tgccgctaat tgtaccactt catcgtattt ccttttcggt
       61 tgaaaccccg ccctttaggg cggcaggatc agactttatt tgggaggggg tgtaacccct
      121 tccaaatcag gacgacacat agggaggtgc tttatgtgtc gtcctgtgtg ttgaaacata
      181 tcggacgcgt ttgaacagtt gtgcggcaaa ctgttttagc ggcaggcaag agatgccgtc
      241 tgaagcccat tgtccgtcat tcccacacag gcagttgcta cggttgctga agccccttag
      301 ccgtcgcgca ggcggttgct acggttacta aaatcccgtc attcccgcgc aggcgggaat
      361 ccagtccgtt cggtttcagt tgtttctgat aaatcttgca gcgttgggtt tttagattcc
      421 cgctttcgcg ggaatgacgg ggcggtcgga tgccgcctga aactccgtca ttcttgcaac
      481 ttttcgtcat tcccgtgaaa acgggaatct agaacttcaa actttcagat aatctttgaa
      541 tattgccgtt gcccgacggt ttggattccc gcttgcgcgg gaatgacggt ggaggacgat
      601 gccgtctgaa gcccattagc ggtttcagac ggcatttttg ccggagcggc agtcaggaaa
      661 actgcgctgc gccacggttt gccaaatcgt cggcgcgttc gttttcggcg tgtcccgcgt
      721 gtcctttcac ccaagtccaa ctgacttgat gccgtccgac tagagcgtcg agttctttcc
      781 acaagtcgtc gtttttgacg ggctgtttgg aggcggtttt ccagccgttg cgcttccaac
      841 cgtgtatcca gttttccatg ccatttttga cgtattgcga gtcggtgcag atgatgacgg
      901 tgcagcggcg tttgagcgat ttcagtcctt cgatgacggc agtcagttcc atgcggttgt
      961 tggtggtttg cgcttcgccg ccgaaaagtt ctttttcgtg gctaccgtag cgcattaaca
     1021 cgccccagcc gcccgcgccg ggattgcctt tgcacgcgcc gtcggtgtaa aggtaaacgg
     1081 tttggttcat tattgtgcct ttgtgtggtg tccggcgtga ttataagcgg tttgcgcgcc
     1141 gtgccgtctg aaagattgcg gcggattaaa ataggcatat aatgtgcaac tttatttttg
     1201 aatatgaagg agcggatagt gggtcaaagt ggcgagttgt tttgtttcgg acagatgcct
     1261 gtatggaaag tggaaaacct gccggaagtt ttgttgtcgg gctattcgtc tgaggaaggg
     1321 gagtgggtct gcctgaatgt gttgcagggc gatgtcgaag ttcgtgcacc ggacgggtcg
     1381 gcagaagttt ggtcggcaga cggcggcgat tgtgtgtttg cgccgcagca ggtgttttct
     1441 gtcaaaccga aaacggacga tgccgaaatc cgtttgtcgc tgtattgcgc ggcggcagac
     1501 tattttcaca aaaaatacgg gatgagtgcc acgcattctg cggtcgcggc ggcacgggat
     1561 accgtaccgg cgggcagggc gttggatatg ggctgcggac aggggcgcaa cgcgctgttt
     1621 ctcggtttga aggggtttga agttactgca gtcgatcaca atcccgccgc cttggcaaac
     1681 gtggcggagc tggcagaggc ggagggtttg aacgtccgca cgctggaata tgatttgaac
     1741 gccgccgcct tgcagggcga gtttgattat attgtggcaa cagtggtgct gatgttcctg
     1801 atgccgcagc gcgtacccga cgtgattgcc gatatgcagg cgcatacggc ggcgggcggg
     1861 tacaacttga ttgtatcggc aatggatacg gcggatttcc cctgtccgat gccgttccct
     1921 tttaaattta aagagggcga gctgaaggat tattatcggg attgggaact ggtcgaatat
     1981 aaagaagaat tgggttctat gcatgccaaa gacgaaaacg gcaatccgat acggtttaaa
     2041 tttgtaacga tgctggcgaa aaagcctgaa taatcctcaa aaatgccgtc tgaacatttt
     2101 gtttcagacg gcatttttac ggttagtccc gatacgccca atgattgacc gggccgtgtc
     2161 ctgcgccgat ttccaaaggg tttgagattg ccgccgtgat gtaggccttg gcagtctgta
     2221 cggcttcgca aacgtccgag ccttttgcca actcggcggt gatgcaggcg gaaaacgtgc
     2281 agcccgtgcc gtgcgtgtgg gcggtcggaa agcgcgggct gtcgaattcc agcgtttcat
     2341 tttgtgtaaa cagccaatcc gtgcagcgtc cgcttgtgct gccgttcaaa tgtccgcctt
     2401 tgataacgac atttttgaca ccgtaatcaa gcaggatttt tgccgcacgt tccgcatctt
     2461 tacggttttc aatatgcacg ccggtcagag cttccgcttc gggcaggttg ggggtcaata
     2521 catccgtatc gggaagcagc aggcgcgtca gtgccgcaac ggcggaatcc tgcaacagcg
     2581 gcgcaccgcc tttggcaatc atcacagggt cgagtacgcg cctgccaaag ctgcagtgtt
     2641 tcagcttgtc ggcaacgcat tcgatgattt ccgccgtgcc gagcataccg attttgtagg
     2701 cgcggatgtc gaagtcttcc ctgattgctt ggatttgtgc ggtgatggtt tcggtcggga
     2761 cgagatgaac cgccgacacg cccaaggtat tttgcgcggt aacggcggtg atgacgcacg
     2821 ttccgaacac gccgcgcatc tgaaatgttt tcaaatccgc ctgaatgccc gcaccgccgc
     2881 ccgaatccga accggcgatg gtaagcgttt gcacaaaaga gcttttcatt tgttcctcct
     2941 taatattgcg tccgataaaa agggctaatg cctgtaaaaa agcacggcga tgccgtctga
     3001 aaaccgcagc gcgggggttt gtggtgcatt atatgccgta ttgcaggaca aggcacaggg
     3061 cagggcggcg gggtgcatcg tagcaactgt atttttgccc gacggggtga aaaatacagt
     3121 tgctacaccc actgataatg aaaaaagaga gcaatgccgt ctgaaccgcg tgttgcggcg
     3181 agcataaacc ggcacgcttt attcgtcatt tccacaaaaa cagaaaccta aaattccgtc
     3241 attcctacga a
//
LOCUS       DAWGRZ010000193         3212 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000193 DAWGRZ010000000
VERSION     DAWGRZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.193"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(63..2225)
                     /locus_tag="WHT44_001884"
     CDS             complement(63..2225)
                     /locus_tag="WHT44_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ2534019.1"
                     /translation="MNTPLFRLSLLSLTLAAGFAHAAENNAKVVLDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTTFGDDDHRIVLSHMKDQHRGIRTVREEFTVGDKSSRINIDR
                     QAPAYRETTQSNTNLAYTGKNLGFVEKLDANAYVLEKERYSADDSGTGYAGNVKGPNH
                     TRITTRGANFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSKFSIPTTEEKNGQKVDK
                     PMEQQMKDRADEDTVHAYKLSNPTKTDTGVYVEAIHDIGDFTLTGGLRYDRFKVKTHD
                     GKTVSSSNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGTK
                     AERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHDSVAVREAVNAGYIKNHG
                     YELGASYRTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRFQN
                     PNLEIGWRGRYVQKATGSILAAGQKDRKGNLENVVRKGFGVNDVFANWKPLGKDTLNV
                     NLSVNNVFNKFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          695 a          712 c          888 g          917 t
ORIGIN      
        1 aaattatcag aacagatgcc gtctgaaagg ctttcagacg gcattttttc gggatgtgcg
       61 ttttagaact tgtagttcac gcccaagcgt acatcacgtc ccacgcccgg cagggtattg
      121 gtccagcgtt ggctgtgcgg atagtagaac ttgttgaaca cgttgttaac cgaaagattg
      181 acattgagcg tgtctttgcc cagcggtttc cagttggcga agacatcgtt cacaccgaaa
      241 cctttgcgta caacgttttc caagttgcct ttgcggtctt tttgacctgc cgccaatatc
      301 gaacccgtag ctttttgaac ataacggccg cgccagccga tttccagatt cggattttgg
      361 aagcggtagg caagggaggc cgtccaagtg cggccgactt gtgcgccaaa ttcaggattc
      421 gcgctcaaca gcttgtcttt gtgcgtatcg taaaagcgcg gtttgctgtg gctgacgccg
      481 actttggcag tcaggccgcc ggtgcggtag gacgcgccca attcgtaacc gtggtttttg
      541 atgtaaccgg cattgacggc ttcacggacg gcgacagagt cgtggcggtt ttgcggattg
      601 gcaagcgcgt ctttgatggt ctgccagaag tagctgccgt ttgcggcaaa cgtgccgtcg
      661 ttgtagttga agccgatttc ggtattgcgc gcgcgttcgg cttttgtgcc gtcggcaatc
      721 gagatgatgc cgcgtttacc gtgggtttgc agcgcgtcat acaggcgcgg gctgcggctg
      781 gcgtagttgt ggctcgcgct gaagctccag tgttcgtgcg gctgccaaat cacaccgaaa
      841 ctcgggttaa ggttgctgct tgaaacggtt ttgccgtcat gggttttcac cttgaagcgg
      901 tcgtaacgca gcccgccggt cagcgtgaaa tcgccgatgt cgtgaatggc ttcaacatat
      961 acgccggtat cggttttggt cgggttggaa agtttgtagg cgtgaacagt gtcttcatct
     1021 gcacggtctt tcatttgttg ttccatcggt ttatcgactt tttgaccgtt tttctcttct
     1081 gtcgtcggga tggagaattt cgagttcaaa aatgcttgcg gtttgatttc ctgatggcgg
     1141 tagttgatac cgtatttcaa cagggtttgt tcggcaaggc ggctgtcgaa gttgaagttc
     1201 gcaccacgag tggtgattcg ggtatggttg gggcctttta cattgcctgc gtagccggtg
     1261 ccgctgtcat cggcggaata gcgttctttt tccaacacat aggcgttggc atccagtttt
     1321 tcgacaaagc ccaggttttt acccgtgtac gccaagttgg tgttggattg ggtagtttcg
     1381 cggtaagcag gggcttggcg gtcaatattt atccgtgaac ttttgtcgcc gacggtaaat
     1441 tcttcacgca cagtgcggat gccccggtgt tggtctttca tgtggctcaa cacgatgcgg
     1501 tggtcgtcgt cgccgaaggt tgttccgatt ttggcgaggt agctgcgttt gtccagcgcg
     1561 ctgtacggta cggttttgcc gccgttgaca ttgcggaaac ctttgccggc ttcgtaatct
     1621 ttttcatcgt tgcggttgta agagaacaag ccgtcgaagt tgccctcttt tccgaatacg
     1681 cttgcgccgt agcttacgcc ttcgttgctg gcaaagccgc tgttgaggcg cacgccccag
     1741 tttttatcca agcctttgag caggtcttgg gcatcgacgg ttttggcgat gatcgcgccg
     1801 ttggtcgcgc cgataccggc agaggcggaa cccgcgcctt tttgtacgga aacgacttta
     1861 accaaagcgg gatcgacaat aaatctgcct tggtggtaaa ggatttggct gtcggaatag
     1921 gcgttgtcca ccttgatgtc gacagagttc tgacccatgc cgcgcagcgt caggaattgg
     1981 gacgtgccgt tgccgccgcc gaaatcgatg gacggctctt cttttaagag ttcgcgcata
     2041 tcggttgcgg tgctttcgtc tttttgttgc agcgtaacga tgttggtacg gattttgctg
     2101 ccttggcggt cgccttttac agtaacggta tccagtacga ccttggcatt attttctgcc
     2161 gcgtgggcaa aacctgccgc aagtgtgagc gagagcaggc tgagacggaa caatggggta
     2221 ttcattcaat cgtcctcttg agtatgaagg gaagtaaatc caaaccgtta agatttggca
     2281 ggataagaaa aataattaat aattattttt atttatatta acaggggggg gggatttttg
     2341 caaagctgat tatcgttttt atttgcgaag tgttgttttt tgttgacagg ttttgtcgga
     2401 aatgtaaaaa aacggcggga atatagtgga ttaacaaatg cgggaatgac gaagcctgcg
     2461 cgggaatgac gaagcctgtg cgggaatgac ggcggagcgg tttctgtttt ttccgataaa
     2521 ttcctaaaac ttaaaatttc atcattcccg caaggacaga aaaccaaaaa cagaaaccta
     2581 aaattcgtca ttcccacgaa agtgggaatc tagaatcccg gactttcaga taatctttga
     2641 atattgctgt tgttctaagg tctagattcc cgcctgcgcg ggaatgacga tatttctgtt
     2701 tttgattttt tgtttttggg gaatgacggg atttgagatt gcgggcattt atcgggtaaa
     2761 acggaaatta tgcgttacga aaatttatcc gaaatcacgg caacttttcc accgtcattc
     2821 ccacgaaagt gggaatccag gtctgtcggc acggaaactt atcgagaaaa acggtttctt
     2881 tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta cggatagtac
     2941 ggaaccgact cactcggtgc ttcagcacct tagagaatcg ttctcttcga gctaagtcaa
     3001 ggcaacgctg tactggtttt tgttaatcca ctatagattt tacgtcctgg attcccgcct
     3061 gcgcgggaat gacgaatttc aattttctgt ttttgatttt ttgtttttac aggaatgacg
     3121 gtcttttcat atcgaaaaaa gttgccgtac cgcaccgata atttccgcct gcgcggaatg
     3181 aagattcaag cgttgcccga aattcaaaaa aa
//
LOCUS       DAWGRZ010000194         3188 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.194, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000194 DAWGRZ010000000
VERSION     DAWGRZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.194"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(75..668)
                     /gene="ribA"
                     /locus_tag="WHT44_001885"
     CDS             complement(75..668)
                     /gene="ribA"
                     /locus_tag="WHT44_001885"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ2534020.1"
                     /translation="MSEMLNHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(661..1338)
                     /locus_tag="WHT44_001886"
     CDS             complement(661..1338)
                     /locus_tag="WHT44_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534021.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGTASIDRSV
                     LWRSDEGWKLADYLSCDNVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENQAFQ
                     LICLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKLLLGMSAG
                     KDGSEDV"
     gene            1492..1576
                     /locus_tag="WHT44_001887"
     tRNA            1492..1576
                     /locus_tag="WHT44_001887"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1526..1528,aa:Leu,seq:tag)
     gene            2096..3130
                     /gene="purM"
                     /locus_tag="WHT44_001888"
     CDS             2096..3130
                     /gene="purM"
                     /locus_tag="WHT44_001888"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ2534022.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYQNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGVGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT          806 a          847 c          793 g          742 t
ORIGIN      
        1 ttattgcttt atcaaggtat ggaaacctgt ttcccgaaag gcggcgcagg atgcccgttc
       61 cctgcaactt tgccttattc cgacatcaga tgtcccagct tgtctgcttt ggtttggagg
      121 tagcgtttgt tttcaaggtt ttccccgacg tgcaggggga tgcgttcgac cacgttaatc
      181 cccgcatctt tcagggtttg gattttttcg gggttgttgg tcagcagttt gaccgagcgg
      241 atgcccagat attcgtagat agattgcgcc aaacggaaat cgcgggcatc gacgggcagc
      301 ccgagtgcca aattggcttc aaccgtatcc ataccttggt cttgcagatg ataggcgcgg
      361 attttgttaa tcagcccgat gccgcgtcct tcctgacgca gatagacgat gatgccgcgc
      421 ccctctgtct gtaccgccct catggccgct tcaagttgag gtccgcagtc gcattttctc
      481 gagaacagcg catcgcccgt caggcattcg gagtggatgc gcgtcagaac cggattgccg
      541 tctgaaaaat taccgacggt cagcgcgacg tgttcctgcc cgtttgcctc ttcaaagccg
      601 tgcatcgtaa atacgcccca ttcggtcggc aggcggcagg atgccacatg gttcaacatt
      661 tcagacatct tcactcccat cttttccggc agacataccc aacagcagtt tcaaaggttc
      721 ggacaaagcc aaggcaagag ccacccattc cacctgtgct gccgtacccg cacactctgc
      781 gcattcaaat tccacatgaa ccacgcccaa cacaccgcca ctttccgtgc agaccggaat
      841 ggaaatttgc gccgccgaag catgattgcg ttctcccgaa agctccccca aatccaacca
      901 acggcgtaca tccgaggcaa cattcatcca accgctttgc gccgaacggc aagccagcga
      961 aaccttgcct gccgcttcat ccaaatccca caggttttcc aaaacctcgc cctgtcggga
     1021 caggcatatc agttggaaag cctggttttc agatggcatc aaggcataga ccgccgcact
     1081 ccgcacgcct gtcgagcgcg aaaacaccga atccaaagcc atgaaaagcc gtttcagtgc
     1141 atcttcgcgg acattgtcgc acgacaggta atcggcaagt ttccaaccct catcactgcg
     1201 ccacaaaacc gaacggtcta tcgaagccgt ccccatatcc attaccgtct gcgccgtcag
     1261 atacgccgcc cgaacctcgt caagcggcag cttcaaaccc tgagtcagca gaaaatcctt
     1321 aatcaaagca gcaggcatac ccaaattccg tcaataaaaa caaaaaaccg cccgattccg
     1381 atgtcaggcg gacgaaaaac agattttacc gccccaaacc ccaagccatc aacagccccg
     1441 ccgaaaacct tgcacaaaaa aacaaaatct gtataatcgt ccaattccca tgcggacgtg
     1501 gcgaaattgg tagacgcacc agatttaggt tctggcgccg agaggtgtga gagttcgagt
     1561 ctctccgtcc gcaccaataa tgataaataa attatatagt taatgcttat cgggacaaat
     1621 tagtgccaca aaacataagt cgtctgcttt tcagcgggcg gcttttttat ctgcctatat
     1681 gtttcaacac gcagaacggt acataaagtg ccgccatttg ttccgcaccg aatcggaaac
     1741 gggtgcttcc gcacgcaatc agattcacca cccctaatgc cggggtttca accggaaaga
     1801 agaaaatatt gattttgatg atggcatcaa aaaacccctt ccgctcgtct gccaaaagga
     1861 atggttaaga aaagcaatgc gtcttttgat ggaacgcgat atataaggaa ttgaggggaa
     1921 gggcaggcgg gtttctgccc gttaatcctg cggacggtcc cgccaaaccc gacacgcgcg
     1981 ggcatatggc ggactgttta caatattgtc taaaaatctg aacctgcaaa cgtgaaataa
     2041 ggtagaatac gccccgttat tttaccgtcc aatccccatt taccaaggaa aaacgatgag
     2101 tacttcattg agttaccgcg atgcaggtgt cgatatcgac gcaggcgacc aactggtcga
     2161 aaacatcaaa ccgtttgcca aacgcacgat gcgcccggaa gtattggggg atttgggcgg
     2221 ttttggcgca ttggtcgaaa tcggcaagaa atatcaaaat cccgtattgg tcagcggtac
     2281 ggacggcgtg ggtaccaagc tcaagcttgc ctttgattgg gataaacatg atacggtcgg
     2341 catcgacctt gttgcaatga gtgtcaacga tattttggtt caaggggctg agcccttgtt
     2401 tttcttggac tattttgcct gcggtaaatt ggatgttccg cgcgcgaccg atgttattaa
     2461 aggcattgcc caaggttgcg aagaatccgg ttgcgccctg attggcgggg aaactgccga
     2521 aatgccgggt atgtatcccg tcggagaata cgatttggcg ggttttgccg tcggcgtggt
     2581 ggaaaaagag aatgtcatta ccggccgcag catcggcgta ggcgatgtgg tattgggttt
     2641 ggcttccaac ggcgcacatt caaacggcta ttcccttatc cgtaaaatca tcgaacgcga
     2701 caatcccgat ctggatgccg agtttgataa tggcaaaacc ttgcgggagg ctgttattgc
     2761 gccgacccgt ctgtatgtga aaccgattct tgccgctttg gaaaaattta ccattaaagg
     2821 tatggcacac attaccggcg gcggcattac cgaaaacgtg ccgcgcgtgt tgcctgaaaa
     2881 cacggttgca caaatcgatg ctaaatcgtg ggaattgccc aagctcttcc aatggcttca
     2941 aaaggcgggc aatgtggaaa cccaagaaat gtaccgaacc tttaactgcg gcatcggcat
     3001 ggtcgttatt gttgctgccg aagatgccga tgcggttcag ggtctcttgg gtgaacaagg
     3061 tgaaacagtt taccgtttag gtttgatccg tgagcgtcag ggagacgagc atcaaaccca
     3121 ggttgcctga ttgcttctat agcgaaatgc cgtctcaaac atgggtttgg acggcatttt
     3181 tatagagg
//
LOCUS       DAWGRZ010000195         3030 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000195 DAWGRZ010000000
VERSION     DAWGRZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.195"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            244..528
                     /locus_tag="WHT44_001889"
     CDS             244..528
                     /locus_tag="WHT44_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ2534023.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     NSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            529..1632
                     /locus_tag="WHT44_001890"
     CDS             529..1632
                     /locus_tag="WHT44_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HEZ2534024.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGIRRKVFTNI
                     KGLKIPHTYIETDAKKLPKSTDEQLSAHDMYEWIKKPENIGSIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGPKLLDQNLRTLVRKHYHIASNKMGMRTLLEW
                     KICADDPVKMASSAFSSIYTLDKKVYDLYESAEVHTVNKVKRSKWFYTLPVIVLLIPV
                     FVGLSYKMLSSYGKKQEEPAAQESAATEQQAVLPDKTEGEPVNNGNLTADMFVPTLSE
                     KPESKPIYNGVRQVRTFEYIAGCIEGGRTGCACYSHQGTALKEVTELMCKDYVKNGLP
                     FNPYKEESQGQEVQQSAQQHSDRAQVATLGGKP"
     gene            1931..2887
                     /locus_tag="WHT44_001891"
     CDS             1931..2887
                     /locus_tag="WHT44_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ2534025.1"
                     /translation="MNIIGLDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKVYIGMEATGIYYEKAADMLSSYYTVYVINPLKIKDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLHQHQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDNNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLNPAIIQSGTSVRGRGRLSRYGNRRLKSTLYMPALCAYRFNAFPKLINN
                     LKKAGKPKMVIIVAIMRKLAKLAYYIVKTGQPYDAERHRLNQ"
BASE COUNT         1043 a          622 c          692 g          673 t
ORIGIN      
        1 gggggtgtgg gggactagtc ccccgcaaag cgttcagctt cggaaacttt ggccgaaagg
       61 caggcgaagc agcgcacttt gcgacgaatg tcgcaaatag ccgagaagcg cggggggatt
      121 ggcgataagc gcgagggggg tgtccccaca gcgccgccgc gccgcgaatg cggcgcaaaa
      181 tctttcagat taagaaacat ttgtttaatg aggcaaccgt gccttttaag aaagggatag
      241 caaatgaaat tgttggccgc attgattccg cttttgatga gcgtggcagg ccgtatattg
      301 actgcattag gcttgatggc ggtaacctat tcaggggtgg atagattggt agcccatttt
      361 cagcaggcga taaccaatag cataacgggc gcgcctcaag cgatgttgca gcttttttat
      421 ataagcggcg gtggaaccgt tcttaatatc ctgtttggcg cgatcgcctt tattctgtca
      481 ttcaaacaaa tgacaaaact agcaacctca atcgggaaga aaaaataaat ggcagagatc
      541 tgtttgataa ccggcacgcc cggttcaggg aaaacattaa aaatggtttc catgatggcg
      601 aatgatgaaa tgtttaagcc tgatgaaaac ggcatacgcc gtaaagtatt tacgaacata
      661 aaaggcttga aaataccgca cacctacata gaaacggacg caaaaaagct gccgaaatcg
      721 acagatgagc agctttcggc gcatgatatg tacgaatgga taaagaagcc cgaaaatatc
      781 gggtctattg tcattgtaga tgaagctcaa gacgtatggc cggcacgctc ggcaggttca
      841 aaaatccctg aaaatgtcca atggctgaat acgcacagac atcagggcat tgatatattt
      901 gttttgactc aaggtcctaa gcttctagat caaaatctta gaacgcttgt acggaaacat
      961 taccacatcg cttcaaacaa gatgggtatg cgtacgcttt tagaatggaa aatatgcgcg
     1021 gacgatcccg taaaaatggc atcaagcgca ttctccagta tctatacact ggataaaaaa
     1081 gtttatgact tgtacgaatc agcggaagtt cataccgtaa ataaggtcaa gcggtcaaag
     1141 tggttttaca ctctgccagt aatagtattg ctgattcccg tgtttgtcgg cctgtcctat
     1201 aaaatgttga gcagttacgg aaaaaaacag gaagaacccg cagcacaaga atcggcggca
     1261 acagaacagc aggcagtact tccggataaa acagaaggcg agccggtaaa taacggcaac
     1321 cttaccgcag atatgtttgt tccgacattg tccgaaaaac ccgaaagcaa gccgatttat
     1381 aacggtgtaa ggcaggtaag aacctttgaa tatatagcag gctgtataga aggcggaaga
     1441 accggatgcg cctgctattc gcatcaaggg acggcattga aagaagtgac ggagttgatg
     1501 tgcaaggact atgtaaaaaa cggcttgccg tttaacccat acaaagaaga aagccaaggg
     1561 caggaagttc agcaaagcgc gcagcaacat tcggacaggg cgcaagttgc cacattgggc
     1621 ggaaaaccgt agcagaacct aatgtacgat aattgggaag aacgcgggaa accgtttgaa
     1681 ggaatcggcg ggggcgtggt cggatcggca aactgaagaa aacggcaaga gagaaaaaag
     1741 acccgtaaac cgtttgaata tagacggttt acgggtcttt gtttcgcgca aagcaagggc
     1801 taaggcagtc aggcagcaaa tcccgcaatg tattaaaaca gacgcgtaga aatgccggct
     1861 gcctttatcc atcctcgaaa ttgaatatca tcctagccgt atcaaggctg tataaataag
     1921 gaaaatacca atgaatataa tcgggctgga catctcaaag gacaccatag acgcaacatt
     1981 gcataaaaca aacggaagta tccattacat taaatttaag aataatgatg atggattaaa
     2041 acagtttaga ttgtggataa agggaaacag aatcagaaaa gtctatatcg gcatggaggc
     2101 aacaggcatc tattacgaaa aggcagcaga tatgctttct tcctactata ctgtttacgt
     2161 tattaatccc ttaaaaatca aggactacgg aaaaagcagg tttaaccgta ccaaaaccga
     2221 caaagcagat tcaaacctga tagcagacta cataaaaagg catcaagata cattgatacc
     2281 gtatcagata cccaaaaaca aagcactgca aaaactgatt aaccttaaaa atcaattaca
     2341 tcaacatcag aagcaaatta aaaaccgtct tcatagcact gaagaagact tcataaggaa
     2401 catacatcaa gacttgatag ataccataca ggacaagatg gaacaggtaa aaatagccat
     2461 atccgaacaa atcaaaaaac aaacggacaa taaccattac cgcaatcttc aaaccatccc
     2521 gagcataggc aaagacaccg catcagttct ttatgcgcaa ctgacagaaa aacattttaa
     2581 aaccgcaaac cagtttgtat cctatgccgg attaaatccc gccatcatac aatcagggac
     2641 aagcgtaaga ggtcggggca gattgagccg atacggaaac agacgattaa aaagtacgct
     2701 gtatatgccc gccctttgtg cttaccgttt taacgcattt ccgaaattaa taaataatct
     2761 gaaaaaagcg ggtaagccaa agatggtaat catcgttgcc atcatgcgca aactggcgaa
     2821 gctcgcctat tacattgtta aaaccggcca gccttacgat gcggaaagac accgattgaa
     2881 tcaataaaat tcaacaaaat taaacggtta cgcgaatata tttgtgtaac cgtgcatttg
     2941 catatcgtaa ataaacgtaa ataaaaataa caatataaat cagtatattg caactttgtt
     3001 ttttattttg tgttgacggg caacatatca
//
LOCUS       DAWGRZ010000196         2969 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000196 DAWGRZ010000000
VERSION     DAWGRZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.196"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            124..1668
                     /gene="purF"
                     /locus_tag="WHT44_001892"
     CDS             124..1668
                     /gene="purF"
                     /locus_tag="WHT44_001892"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ2534026.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAQVHRLVRGAYGVIAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALTYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            1768..2259
                     /gene="greB"
                     /locus_tag="WHT44_001893"
     CDS             1768..2259
                     /gene="greB"
                     /locus_tag="WHT44_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ2534027.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRDDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            2318..2944
                     /locus_tag="WHT44_001894"
     CDS             2318..2944
                     /locus_tag="WHT44_001894"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ2534028.1"
                     /translation="MRKIRTKICGITTPEDAAAAAAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFHRPYIK
                     AIRVQTASDIRNAATRFPDAQALLFDAYHPSEYGGTGNRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAESVDVSGGVEASKGKKDAAKVAAFIATANRLSR"
BASE COUNT          713 a          905 c          776 g          575 t
ORIGIN      
        1 tcttattctc cataaaccca cctatctgtc cttcgctcaa atattgtcaa caattcaggc
       61 aaaaaaccac cgtttacttt ataattccgt tttaccctta aacctcgaat cggagaagac
      121 gatatgtgcg gcgtattagg tttggtcagt catgaacccg tgaaccagct tctgtacgac
      181 ggcttgcaga tgttgcagca cagggggcag gatgcggcgg gcattgcaac ggcggaaggc
      241 ggtaccttcc atatgcacaa aggcaaaggc atggtgcgcg aagtgttccg cacacgcaat
      301 atgcgcgatt tgaccggcaa cgccggcatc gcccacgtcc gttatcctac tgcgggcaac
      361 gcaggcagca gcgcggaggc gcagcctttc tacgtcagct cgccgttcgg catcgttttg
      421 gcgcacaacg gcaacctcac caacactgcc gaactgtatg aaaacgtgtg taacaaacac
      481 ctgcgccacg tcaacaccag ctccgattcc gaagtcttgc tcaacgtatt cgcgcacgaa
      541 ttacgccgcg aagtctctaa aaacgccgac ccgcaccggc tcaatgccga caatattttc
      601 aacgccgttg cccaagtcca ccgcctggtg cgcggcgcat acggtgtgat tgccatgatt
      661 gcgggctacg gtatgctcgc cttccgcgac ccttacggca tccgcccgct ggtattgggt
      721 tcgcaaaccg acagcgaagg cagaaaatcc tatgccgtcg cctccgaatc cgtcgccttc
      781 aacgcgctta cctacgattt ggaacgcgac atccggccgg gcgaagcggt atttgtcggc
      841 tttgacggca caatgattgc ccgccaatgc agcgaccgtg ccaaactcag cccctgcctt
      901 tttgaatatg tttattttgc ccgccccgac tctgtgattg acggcgtatc cgtttaccaa
      961 tcgcgcttgg atatgggcgt gtcgctggcg gaaaaaatca aacgcgagct gcccgtggac
     1021 ggcatcgatg tcgtgatgcc cattcccgac accagccgcc ccagcgcgat ggagcttgcc
     1081 gtccatctcg acaaacccta ccgcgagggt ttgattaaaa accgctatat cggccgcacc
     1141 tttattatgc ccggacaggc gacgcgcaaa aaatccgtgc gccaaaaact cagcccgatg
     1201 gaaaccgaat ttgcaggcaa aagcgtgttg ctggtggacg actccatcgt gcgcgggacg
     1261 accagccgcg aaatcgtcga aatggtacgc gcggcgggcg cgcgcaaagt ctatatcgcc
     1321 tccgccgcgc ccgaagtgcg ctatcccaat gtgtacggca ttgatatgcc cacgcgcgaa
     1381 gagttgattg ccaacgggcg cagcgcggcg gaaatcgccg ccgaaatcgg cgcggacggc
     1441 atcgtatttc aagacttggg cgatttggaa gccgtcgtca aagcactcaa tccgaaaatc
     1501 gaatccttcg attcgtcctg tttcaacggc atttatcaga ccggcgacat cgatgatgcc
     1561 tacctcgacc gcctgtctgc cgagaaatcc ggctgcggcg gtctgaaaat ccacccgagc
     1621 aggatggaac acagcatcag catcagcgat gcgggcgacg aagaataagg cttcccaaac
     1681 cgcgatgccg tctgaaatcc gaacgccggg ttttcagacg gcatttccca tatcggcatc
     1741 acatttcccg tttcatccgc acacaatatg agtaccgaaa ccaaaaacta cattacgccc
     1801 gccggatggc aggcattgaa agacgagctt tatcaactgg tcaacaaaga acgccccgaa
     1861 atcgtccaaa tcgtcaactg ggcggcaggc aacggcgacc gcagcgaaaa cggcgactat
     1921 ctttacggca aacgccgtat gcgcgaaatc gaccgccgca tccgtttcct gaccaaacgt
     1981 ttggaggctg ccgtcgtcgt cgatcccgaa ttgcgcgaag cgaccgacca agtgtttttc
     2041 ggcgcgacgg tcggactgtt gcgcgacgac gggcgcgagc aaaccgtcaa aatcgtcggc
     2101 atcgatgaaa tcgataccgc gcaaaacaaa atttcttgga tttcgccgct ggctcgttgt
     2161 ctgattaaag cgcgcgaagg cgacgaagtg gttttgaaca cgccggaagg gcgtgaggaa
     2221 attgaaatcc tttctgtcga atacatcaaa atcgactgat cgggaatgcc gtctgaaacg
     2281 gcaaaagcgc gccgtttccc aaataaagga ataccgaatg aggaaaatcc gcaccaaaat
     2341 ctgcggcatc accacaccgg aagacgcagc tgccgccgca gcggcaggtg cggatgccgt
     2401 cgggctggtc tttttccaag gcagcagccg ggccgtcgat attgcccgcg ccaaaaaaat
     2461 caccgccgca ctgccgccgt ttgtcagcgt tgtcgccctt ttcgtcaacg aaagcgcgca
     2521 aaacatccgc cgcatccttg ccgaagtgcc gatacacatc atccaattcc acggcgacga
     2581 agacgacgca ttctgccgcc agttccaccg cccctatatc aaagccattc gtgttcagac
     2641 ggcatcagac atccgaaacg ccgccacgcg cttccccgac gctcaggcac tgctgttcga
     2701 tgcctaccat ccttcggaat acggcggcac cggaaaccgc ttcgactgga cgctgctggc
     2761 ggaatattcg ggcaaaccgt gggtgcttgc cggcgggctg acccctgaaa acgtcggcga
     2821 agccgtccgc atcaccggag cggaatcggt cgatgtatcc ggcggtgtgg aagcgtctaa
     2881 aggcaaaaaa gatgccgcca aagtcgccgc ctttatcgca accgccaacc gcctatcccg
     2941 ttaaagcaac aaaaattgcc gccggaatg
//
LOCUS       DAWGRZ010000197         2949 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000197 DAWGRZ010000000
VERSION     DAWGRZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.197"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            32..1927
                     /gene="mnmG"
                     /locus_tag="WHT44_001895"
     CDS             32..1927
                     /gene="mnmG"
                     /locus_tag="WHT44_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ2534029.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLSHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGERISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNADMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIAGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVSEAQWRMFNEKREAVEREIQRLKTTWYTPQKLAEGEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            1996..2580
                     /gene="rnhB"
                     /locus_tag="WHT44_001896"
     CDS             1996..2580
                     /gene="rnhB"
                     /locus_tag="WHT44_001896"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ2534030.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVEWHVAAASPEEIASLNILHATMLAMKRAVDGLAVRPEKIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALEKYGVLPEHRRDFAPVRNLLAQQALF"
BASE COUNT          793 a          939 c          720 g          497 t
ORIGIN      
        1 ccgtctgaaa caccgtcagg caatacacac tatgacccac atgatttacc ccaaaaccta
       61 cgacgttatc gtcgtcggcg gcggacacgc cggcacggaa gccgcactcg ccgccgcccg
      121 tatgggcgcg cagacgcttt tgctctcaca caatatcgaa acgctcggac aaatgtcgtg
      181 caacccctct atcggcggca tcggcaaagg gcatttggtg cgcgaactcg acgcgctcgg
      241 cggcgcgatg gcgttggcaa ccgacaaatc cggtatccag ttccgccgcc tgaacgccag
      301 caaaggcgcg gcagtgcgtg ccacgcgcgc gcaggcggac cgcatcctgt acaaagccgc
      361 catccgcgaa atgttggaaa accaagaaaa cctcgacctt ttccaacaag ccgtcgaaga
      421 cgtaacgctc gacggcgaac gcatcagcgg cgtaattacc gcgatgggcg tggagtttaa
      481 agcacgcgcc gtcgtgttga ccgcaggcac gtttttgtcc ggcaaaatcc acatcggttt
      541 ggaaaactac gaaggcggac gcgccggcga ccccgccgcc aaatcgttgg gcggacgttt
      601 gcgcgaattg aagctgccgc aaggccgtct gaaaaccggc acgccgccgc gtattgacgg
      661 acgcacgatt gacttctccc aactgaccga acagcccggc gacacgcccg ttcccgtcat
      721 gtccgtgcgc ggcaacgccg atatgcaccc gcgccaagtg tcctgctgga ttacgcatac
      781 caacacgcaa acccacgaca tcatccgctc aggcttcgac cgcagcccga tgtttaccgg
      841 caaaatcgaa ggcgtgggtc cgcgttattg tccgtctatc gaagacaaaa tcaaccgctt
      901 cgccgacaaa gacagccacc agattttcct cgaacccgaa ggtctgacca cgcacgaata
      961 ctatcctaac ggtatctcca ccagcctgcc gttcgacatc caaatcgcgc tcgtccgcag
     1021 tatgaaaggt ttggaaaacg cccatatcct gcgccccggc tacgccatcg aatacgacta
     1081 cttcgatccg cgcaacctca aagcaagcct cgaaaccaaa accattgccg gattgttttt
     1141 cgccgggcaa atcaacggta cgaccggcta cgaagaagcc gccgcgcaag gtttattggc
     1201 aggcgcgaac gccgtgcaat atgtgcgcgg acaagacccg ctcctgctgc gccgcgaaca
     1261 agcctacctc ggcgtattgg tggacgacct catcaccaaa ggcgtgaacg aaccctaccg
     1321 aatgttcacc agccgcgccg aataccgcct gcaactcagg gaagacaacg ccgacatgcg
     1381 cctgaccgaa gacggctaca aaatcggctt ggtgtccgaa gcgcaatggc gcatgttcaa
     1441 cgaaaaacgc gaagccgtcg aacgcgaaat ccaacgtttg aaaacaacgt ggtacacgcc
     1501 gcaaaaactc gccgaaggcg aacaaatccg tgtgttcgga caaaaactca gccgcgaagc
     1561 caacctgcac gacctcctgc gccgcccaaa cctcgactac gccgcgctga tgacgctcga
     1621 aggcgcgatg ccgtctgaaa acctctccgc cgaagtcatc gaacaagtcg aaatccaagt
     1681 caaataccaa ggctatatcg accgccaaaa cgaagaaatc gacagccgcc gcgacatcga
     1741 aaccttaaaa ctgcccgacg gcatcgatta cggcaaagtc aaaggcttgt cggcagaagt
     1801 gcagcaaaag ctcaaccagc acaaacccga aaccgtcgga caagccagcc gcatttccgg
     1861 cgtaacccct gcggcagtgg cattgctgat ggtgcatttg aagcgcgggt ttaaagacgc
     1921 gaaataaaca catcggcgcg atgccgtctg aaaccctttt cagacggcat tcccaccatc
     1981 ccgacaggaa acatcatgca catactgacc gccggcgtgg acgaggcagg acgcggacct
     2041 ttagtcggca gcgtgtttgc cgccgccgtc atccttccgg aaacattcga cctgcccgga
     2101 ctgaccgact ccaaaaaact cagcgagaaa aaacgcgacg cgcttgccga aatgataaaa
     2161 aatcaggcgg ttgagtggca cgttgccgcc gcctcgcccg aagaaatcgc cagcctcaac
     2221 atcctgcacg ccaccatgct cgcgatgaaa cgcgccgttg acggcttggc tgtgcgtccc
     2281 gaaaaaatat tcatcgacgg caaccgcatt cctgaacatt tgaacatccc tgccgaagcc
     2341 gtcgtcaaag gcgacagcaa aatcatcgaa atctccgccg catccgtttt ggcaaagacc
     2401 gcacgcgatg cggaaatgta cgcactggcg caacgccatc cccaatacgg tttcgacaaa
     2461 cacaaaggtt acggcacgaa gcagcatttg gaagccctcg aaaaatacgg cgtgctgccc
     2521 gaacaccgcc gcgacttcgc ccccgtcaga aacctgctcg cgcagcaggc cttgttttaa
     2581 accggcacaa aaatgccgtc tgaagccttc agacggcatt tccggcatcg caactccaaa
     2641 gagaaagaac ccaaaccgtc attcccgcga aaaatagaaa atcaaaaaaa aaacctaaaa
     2701 tccgtcattc ccgcgcaggc gggaatccaa tccgtccggt ttccgttttt tttttgaatt
     2761 tcaggtaact tccaaaccgt cattcccgcg aaagcgggaa tctagaaact caaagctgca
     2821 agaatttatc aaaaatgact gaagctcaaa aaaccggatt cctacgaaaa caggaatccg
     2881 gagtctcagg gctggcaaaa ccgttttacc cgataagttt ccgtaccgac agacctagat
     2941 tcccgcctt
//
LOCUS       DAWGRZ010000198         2946 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.198, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000198 DAWGRZ010000000
VERSION     DAWGRZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.198"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..317
                     /locus_tag="WHT44_001897"
     CDS             <1..317
                     /locus_tag="WHT44_001897"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534031.1"
                     /translation="KNIPLKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAGF
                     NGDIGEKLHRCAVMEWAENETVKLAQKWEQEQKKQQIQQKKETEKSPKHKASRDDWEM
                     ER"
     gene            complement(399..1970)
                     /locus_tag="WHT44_001898"
     CDS             complement(399..1970)
                     /locus_tag="WHT44_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEZ2534032.1"
                     /translation="MLILAISLEVFALVSPFFMQWVIDHVIVTADKNLLLTLTLGFGL
                     LTILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSI
                     DHIQETLTSTFFVLVLNSLMAVFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLR
                     NATEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLS
                     ALFEFSNKLLFSMENVIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYI
                     QIKMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFE
                     NINLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGL
                     SGIVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDM
                     GNILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAH
                     RQETIQSADKILNLG"
     gene            complement(1980..2485)
                     /locus_tag="WHT44_001899"
                     /pseudo
     CDS             complement(1980..2485)
                     /locus_tag="WHT44_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815283.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
     gene            complement(2488..2946)
                     /locus_tag="WHT44_001900"
                     /pseudo
     CDS             complement(2488..2946)
                     /locus_tag="WHT44_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
BASE COUNT          972 a          496 c          517 g          961 t
ORIGIN      
        1 taaaaaatat ccctttgaaa gattttgatg aagtttgtga aaagtgggag aaatccttta
       61 aagaaaatgg aaaagaaatt gaatataatc aaaaatattc aagaatttat ggtgaagttc
      121 gtggagctgg ctttaatggc gatatcggag aaaagctaca tcgttgtgcc gtgatggaat
      181 gggctgaaaa tgagaccgtg aaattagccc aaaaatggga acaagagcag aaaaaacaac
      241 aaattcaaca gaaaaaggaa actgaaaaat cgccaaaaca caaagccagt cgtgatgatt
      301 gggaaatgga acgttaaacc gctattttca tgtattctat taaattattt gaaaaaagac
      361 ttcacttttt gttaaagaaa aatgaagtct tgttctgttc aacctaaatt cagaatttta
      421 tctgccgatt gaattgtttc ttggcggtgt gcaaccatta ttttcataat accaagactt
      481 tttaggttat ggttaatttt ttgttcattt tctacatcta aatggctact tgcttcgtct
      541 aaaaatagaa ttttgggtcg tttatacaat gcacgagcca agataactct ctgcttttgt
      601 ccacctgata agatatttcc catatcgcca atcaaggtct catagcccat tggcatttta
      661 agtatatcgt catgtatttg tgccattttt gcacattgtt caatgagctc catatttggg
      721 ctttcatcaa aaaatgaaat attttcccca atagaacctg caaaaaggac atcatcttgg
      781 cgaacaatcc cgctcaatcc cctaataaag gatggagaaa cttgatatat atcatgccca
      841 ttaatactaa ctgtaccagt ttcaggtttt aggctacctg ttaaaatgtt taacaaagtg
      901 gacttccccc gaccagattg tcctgttaaa acaactgctt cattatcttt aaattccaaa
      961 ttaatgtttt caaaaagata tggctcatta tcagcatatc tgaatgagac gttttcaact
     1021 ttaagaacca gttgttcatt atctaattta ggtatatgat tatacttaat aatttcagtt
     1081 tctgtttcat ttaaagtaat gtcagccaaa cgttcagcat gaagccctaa cattttgatt
     1141 tggatgtatt ggtcaacgag agaagctgtt ctgctttcaa attgcccttt ataagccaaa
     1201 aaagccatca gaacaccgac tgtaaatgaa ccatctaaaa ttgcgcttgc accaagataa
     1261 attatgataa cattttccat gctaaacaac agtttatttg aaaattcaaa taaagcagag
     1321 agtttatctg ttgtcagctt ggtattgact gtattcacaa ataggctcat ccaagtgcca
     1381 tgtctttgat aatgtttatc aaataattta actgattgga taccacgaat ggtttccatg
     1441 aaatatgagt tttgtttggc ttcatgaaca atattttctt ctgttgcatt tcttaatggg
     1501 taatatgcaa gccaacgaat tagtatgtac aaaacaagtg ttaaaagaac aatcagcgat
     1561 aattgagtgc tgtaaattgt cattaacacg aaagtaaaaa cagccattaa gctatttaaa
     1621 actaaaacaa aaaaagtaga agttagtgtt tcttggatat gatctattga accaaatctt
     1681 gaaatcacat ctcctaaatg tcgtttactg aaatagtcat taggtaagtc aagtaacctt
     1741 ttaaatatat tggctttcca ttgtaaatta agagttgtag ataggtgcat acctacccat
     1801 gcttgtaaca ggctaattaa ctgttgcagg atagtcagta aaccaaatcc caaagtaagg
     1861 gtcaataata aatttttatc agcagttaca atgacatggt ctattaccca ttgcataaag
     1921 aatggactaa ccaatgcaaa gacttccaaa gaaatagcta atataagcat ttgaattaaa
     1981 gagcgtttta agcctgaccc ccccttaata gagataatat tttgattttc tttgtttctt
     2041 ttttctcttc aaaatgggta ttggggaata attctagggc aatccctgtg aatttttgtg
     2101 aaacttcgtc cattttgatt tttcgcatac cgacagcagg gtccataatg acgatactgt
     2161 ctttggaaat ggaacaaagt acaacaaaat ggtttaagtt ccaatggaga atgcagggta
     2221 gttgtaaatt tgacagctca tctaactcta aacgcaaagc tcgtggcgtt aaattcattt
     2281 cattgccaaa tctcatgatg tctgcaagat ttgcgccctt taatgacagg gtgtattttt
     2341 ggcgtaacgt tcttaaatca gtgtgaaagc cataatagga caagatggat gtcaggcatg
     2401 ctaaaccaca ttcagcaact tctgtttgca gaatgacagg tagctttttg ttaaatccaa
     2461 aggacagtct tgataaataa tccattttta attgattttt cctgaaatgc tgtaaagtgg
     2521 gtcaagtacc cattcgtaca attttttcgt tcatggagaa tatctgcttc taaaatcatg
     2581 ccaatttgaa gcggcttgtt ttctccgtat gctttaatcg tttgtttttc caatttaact
     2641 ttcacaagat aggcaggttc atttaataag gttgggttag tgaaaatgat acctaaacct
     2701 gatagctttt gtttaccgag agcagttctg gcaactgaaa taatttctcc tgtggcatgt
     2761 ccaaattttt ggtaagggta cgcttggtaa cgtaaaacaa ctttatcttt cggtttaata
     2821 aaaccaacag ctttactggg tatgtaaaga ttggcgacca attcagtttg ttcagggaca
     2881 atgcttaaca gcaatttaga cggttcaact tgttgcccta tatcaacatt aattgttgat
     2941 atataa
//
LOCUS       DAWGRZ010000199         2925 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.199, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000199 DAWGRZ010000000
VERSION     DAWGRZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.199"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..806)
                     /locus_tag="WHT44_001901"
     CDS             complement(<1..806)
                     /locus_tag="WHT44_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin D"
                     /protein_id="HEZ2534033.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKREQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSG"
     gene            complement(820..990)
                     /locus_tag="WHT44_001902"
     CDS             complement(820..990)
                     /locus_tag="WHT44_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534034.1"
                     /translation="MNTETIYATVFCILAATISGLLVKFNVIKIETSINSKFMLLGIS
                     ILIIGIFLSIFF"
     gene            complement(2031..2267)
                     /locus_tag="WHT44_001903"
     CDS             complement(2031..2267)
                     /locus_tag="WHT44_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ2534035.1"
                     /translation="MKELHTSELVEVSGGKFHIFAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGISKKW"
     gene            complement(2777..2925)
                     /locus_tag="WHT44_001904"
                     /pseudo
     CDS             complement(2777..2925)
                     /locus_tag="WHT44_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          931 a          514 c          496 g          984 t
ORIGIN      
        1 cctgatttac tagctcgtat ggtttgttcg gatttcaaat caaaatccaa aatttcttgg
       61 ttcatttccg taatcgcacg gttgagttgg ctcaattcgg ttttatggcg ttcaggcagg
      121 ctgcttaatg tgattttctg ttcatcaagt tccctgattg cattattttg ttcacgtttt
      181 aggctctcca aacgtgagcg ttgttccaat aaatggcttt cggcggtcat cttatcttgt
      241 tgggatactg cgccttgact ggctaaaaac ttgttcttgt taagggtttt ttccgctaaa
      301 cgaatttgac gattttgccc tgtaatttgc tgtttaatat tctctaattg attgtttaaa
      361 cgatgaatgt tgttatgaac atttttttgc tcattttgat gaatgcgctt taaacgttcc
      421 aattcttgta atgccaaagt ttttttaagg ttggcttctg ctgccaattt ggcttgtacg
      481 tttccttttt cgccaaaacg cgatgtggaa agtttgaaca atggttcgcc agctttgaca
      541 aagttaccat cttcaacaaa tttatgcgta atcgtgccga tatcggaaga gtaaacacga
      601 accaccccca tagttggaag taattgacct tcaacggttg ttttattggt atagctacca
      661 aaaatcaaaa agataatgat acacagagca atgagaaaag cgcaaaaagt cagaaataaa
      721 aaagagaatg gacgggtcaa gataacctga cctgtccact tattttgttg ggctacaaag
      781 acttcttttc ggaataagtg ggacatttat tattatttct taaaaaaaaa tggatagaaa
      841 aataccaata attaaaatac ttatgcctaa taacataaat ttgctattga ttgatgtttc
      901 tattttaatt acattaaatt taaccaataa tccagaaatg gttgcagcta aaatgcaaaa
      961 gacagtggcg taaatggttt cagtattcat aatttttctc tttcaaaatt gttcaatcta
     1021 aatagaaaaa ggtaacttca atacatattg ctgtaactga aattaccttt tccttaatga
     1081 tgtaattatc ttatggcttc atcatgccac gaccgatatt gtatccgcca gcaacagaga
     1141 aaataccagc tcccattgcg tcaacccagc ctactggtcc tgtccacttg ttccgttgag
     1201 ctacaaagac ttctggtctg aaaaatgagt gattgttaga catgtgtact tatttatgtt
     1261 tctttgattg ggataaattt gtaaaaacaa aaaaaaggca agttgttatt atagaagaac
     1321 ttaaaatgct taacatactc attgaggttt gtaccacata atgagatacg agataatttg
     1381 ttgggagtaa aatcaaaaaa aagattaaat atttaaaata atcactatat tttttcatta
     1441 tttgttcctt tttgtattta agttcttatg aaaaactagg gtattttgaa tagaaaatat
     1501 caaaataccc tagttttatt aaggaatatt accaccaaca agtgcttcca tattgtacag
     1561 caccagtgat tgcaccacca atagctccga atttcgcacc tggaatagca ccaataccac
     1621 ctgcaaatga accaacaatt gccccaccag ctgctccacc taatgcacta ccaatggtat
     1681 tttttgagaa atcacgccag ttacaagcag caccagaaac ttgttaaatt tcattcaaag
     1741 tcaaaacttg cataatattc acctttgtta agtgtggttg agaaaaaagt aaattttcat
     1801 cgttactcat tatttgcaat catgcatata ctgtaaccaa gcactatatt cagttggatc
     1861 atagtttttc ctactgggtt gtctgatatg actattggca ctattggcac aattgggatt
     1921 agcaacttta ttgtgagtac ctaatggatt gatatttcct gtaaagtggc tattgcatgc
     1981 gcttaatgag catagattta ttaatgtcag aaaggataaa acaaaaaatc ttaccatttt
     2041 ttactaatac caaaagtagg tttcgaaaac tgtacaccaa gaccttgtac atatccaaat
     2101 tgcttgctaa cagaaaactc tactccacta ttgttagggg aataggaagc accaatttta
     2161 ccaacagcaa ccatatcttt tttacctagg ttgccgccac cctgtgcaaa gatatggaat
     2221 ttgccacctg acacttcaac taattcagag gtgtgtaact ctttcatgat tttctcctgt
     2281 aaagaagtat ggttggaaat gtaaatcaga attaaccttt cttaagttta atttaatact
     2341 ttttttgcaa gtattttatt ttaaattaaa tcaatatttt aaaaatattt acttgtatac
     2401 tgaaaaagaa actcctaatg gtggagtggg gcttttgttt aagtaagcat taaaaaaatt
     2461 taaatttccc attatgatta acttaatgca agagcttttt caggacgtac aaaccagcga
     2521 acgggcgttc tgatgtgggc agcctgccgg gaagtctgct ctattgttcg gacaataggg
     2581 ggaatatacc aattacttta actacatcat tttttgacat ttttcttgcc tttcaattta
     2641 aaatccaaaa tcatactgcc ataatttagc aatccaaaaa aatttaggca gcagtaatgg
     2701 tttcgtattt tagaaaacga aacttggttt ggtgttttga agcatttcag tacacaaacg
     2761 gggctgtcct agataactaa gataaactcg attttactaa ttgttttaaa atggaacaag
     2821 aacttttatc tcactgttgt taaaacgtcg ttcgcactcc tttaaataca gctcaaaatg
     2881 cgctttggga atgccgttag acttgcgtaa atgacgtttt gcctg
//
LOCUS       DAWGRZ010000200         2893 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.200, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000200 DAWGRZ010000000
VERSION     DAWGRZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.200"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(125..1117)
                     /gene="pdxA"
                     /locus_tag="WHT44_001905"
     CDS             complement(125..1117)
                     /gene="pdxA"
                     /locus_tag="WHT44_001905"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ2534036.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGASEEGVLEVLHIPAAEAVEAGKLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLNKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            1277..2704
                     /locus_tag="WHT44_001906"
     CDS             1277..2704
                     /locus_tag="WHT44_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ2534037.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
BASE COUNT          575 a          789 c          828 g          701 t
ORIGIN      
        1 ccaatggtct ggattcccgc ctgcgcggga atgacgattt ggaaattacc cgaaacccaa
       61 aaacaactga aaccgaacag accggattcc cgcctgcgcg gaaatgacgg gtcttttatc
      121 atctttaaag gctgccgcgc gccatctcga cggcggtctc cacggcagtt atcaggctgc
      181 cggaatccgc cctgccggtt gccgccaaat caagcgcggt gccgtgatcg acggaggtgc
      241 ggataaaggg caggccgagc gtgatgttca cgccctgtcc gaagctgtgg tatttcaaca
      301 cgggcagccc ttggtcgtgg tacatcgcca atacggcatc cgcaccttcg agcataaacg
      361 gttggaacaa ggtatccgcc ggatacgggc cggcgaggtt tatcccttcg cggcgcaggt
      421 tttcgagcgc ggggataatg gtgtcggttt cttcgtgtcc gaggtgtccg ccttcgccgg
      481 cgtgggggtt gagtccggcg actaggattt tgggattttt gatgccgaat ttgtgtttta
      541 agtcgtgatg caaaatgcgt gcgacgcttt caatcagcgg ttgcgtgatg gcggcggcaa
      601 cgtctttcag cggcaggtgg gtcgttacga gggcgacgcg caggcctttg ccggcaagca
      661 tcatcacaac ctgccccgtg ccgctttttt ccgccagata ttcggtgtgt ccgctgaaaa
      721 aacctgtgct tgcgcgcgcg tcgttgatga tgcctttgtt cagcggcgcg gtaacgatgc
      781 cgtcgaaaat gccgtctgaa atgcctgcga gcgcggtgtc tagaagttgc agcacatagg
      841 cggcgttggc gggattgagt ttgcccgcct caaccgcttc ggcggcaggg atgtgcagca
      901 cttccagcac gccttcttcg gacgcgccgc ctgattccgg atcgaagtcg cgcaggacga
      961 cgcttttgcc caaggcttcg gcgcgcgcgc gcaacaggtt tttgtcgccc aataccgcgc
     1021 agcggcaggg caggcgtgca aatgccaagt ccaaacaaat atcggggccg atgccggcag
     1081 gctcgccgga agtaacggca aaaacaggct gtttcatcgt gtttgctcca aacaaaatgc
     1141 gattctaacg ccgcagccgc gcggcgatgt aaatttttct gattttgttg acaatctgct
     1201 agaatgggcg tttacaaaat ttaaaccctg cttgcatacc gccaatatgt gcgagtttca
     1261 actttaagga agcgatatga acgagaactt taccgaatgg ctgcacggct gggtcggcgc
     1321 catcaacgat ccgatgtggt catacttggt ttatatgctt ttgggtacgg ggcttttctt
     1381 caccgtaacc acgggctttg tccaattccg cctgttcggg cgcagcatca aagaaatgct
     1441 cggcggccgc aaacaggggg acgaccctca cggcatcacg ccgtttcagg catttgtaac
     1501 cggccttgcc agccgcgtgg gcgtgggcaa tatcgcgggc gtggccatcg ccatcaaagt
     1561 cggcggaccg ggcgcggtgt tttggatgtg ggtaaccgcc ttaatcggta tgagttcggc
     1621 gtttgtcgaa tcttcgctgg cgcagctctt taaagtccgc gactacgaca accaccattt
     1681 ccggggcggc cctgcctact acatcactca agggctgggg cagaaatggc tgggcgtgtt
     1741 gttcgccctg agcctgattt tctgtttcgg ctttgtgttt gaagcggttc agaccaatac
     1801 catcgccgat accgtcaaag cggcatgggg ttgggagcct cattatgtcg gcgtcgccct
     1861 ggtgatttta accgcgccga ttatcttcgg cggcatcagg cgcatatcta aagcggcgga
     1921 aatcgtcgtc cccctgatgg cggttttgta cctctttatc gcgcttttca tcattttgac
     1981 caatattccg atgattccgg acgtgttcgg tcagattttt tcgggcgcgt tcaaattcga
     2041 cgcggcagca ggcggcttac tcggcggtct gatttcgcaa acgatgatga tgggcatcaa
     2101 acgcggcctg tattccaacg aggcgggtat gggttccgcg ccgaacgccg ccgccgccgc
     2161 cgaagtgaaa caccctgttt cgcaaggtat gattcaaatg ctgggcgtgt ttgtcgatac
     2221 catcatcgtt tgttcttgca ccgccttcat catcttgatt taccaacagc cttacggcga
     2281 tttgagcggt gcggcgctga cgcaggcggc gattgtcagc caagtggggc aatggggcgc
     2341 gggcttcctc gccgtcatcc tgtttatgtt tgccttttcc accgttatcg gcaactatgc
     2401 ctatgccgag tccaacgtcc aattcatcaa aagccattgg ctgattaccg ccgttttccg
     2461 tatgctggtt ttggcgtggg tctatttcgg cgcggttgcc aatgtgcctt tggtctggga
     2521 tatggcggat atggcgatgg gcattatggc gtggatcaac cttgtcgcca tcctgctgct
     2581 ctcgcccttg gcgtttatgc tgctgcgcga ttacaccgcc aagctgaaaa tgggcaaaga
     2641 ccccgagttc aaactttccg aacatccggg cctgaaacgc cgtatcaaat ccgacgtttg
     2701 gtaaatcccg cccttaccgg agccgcttcc ccgcgaagcg gcttttccct ttccgcacac
     2761 tgtaaaaaca gggcgaacaa gcgtacaatc ccaacccttt acttttgaat ccatttcgtt
     2821 tttcagacgg catattgaat atagtggttt aacaaaaatc aggacaaggc gacgaagccg
     2881 cagacagtac aag
//
LOCUS       DAWGRZ010000201         2830 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.201, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000201 DAWGRZ010000000
VERSION     DAWGRZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.201"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            32..463
                     /locus_tag="WHT44_001907"
     CDS             32..463
                     /locus_tag="WHT44_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ2534038.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQEGEQDRRKRLLSLTETGKAYAAPLTE
                     SAQEFSDKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            474..1340
                     /locus_tag="WHT44_001908"
     CDS             474..1340
                     /locus_tag="WHT44_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEZ2534039.1"
                     /translation="MWKMLKHIAQTHRKRLIGTFSLVGLENLLMLVYPVFGGRAINAV
                     IAGEVWQALLYALVVLLMWLVGAVRRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLNNSLERDNHFIRKGDRRQLYRHYGLLARLRVLISNREAFGYLCVG
                     TAMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQR
                     IEWSERNIKAGT"
     gene            complement(1391..2443)
                     /gene="sohB"
                     /locus_tag="WHT44_001909"
     CDS             complement(1391..2443)
                     /gene="sohB"
                     /locus_tag="WHT44_001909"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ2534040.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIASAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(2547..2636)
                     /locus_tag="WHT44_001910"
     tRNA            complement(2547..2636)
                     /locus_tag="WHT44_001910"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2600..2602),aa:Leu,seq:taa)
     gene            complement(2739..2812)
                     /locus_tag="WHT44_001911"
     tRNA            complement(2739..2812)
                     /locus_tag="WHT44_001911"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2778..2780),aa:Cys,seq:gca)
BASE COUNT          681 a          764 c          675 g          710 t
ORIGIN      
        1 cgcacaaatt tagtcaatat caagaccaat tatgaaccaa ctcgaccaac ttggcacccg
       61 tatcaacctg atttgcaatg tcttcgacaa atggatcggg cagcaggatc tgaattacaa
      121 cctctttgcc gtactttata ccctggcaac cgaaggcagc cgcacgcaaa agcatatcgg
      181 cgaaaagtgg agcctgccca aacagaccgt ttcaggcgta tgcaaaaccc ttgccggaca
      241 agggttgatt gaatggcagg aaggcgaaca ggaccggcgc aaacggttgc tgtcgttgac
      301 cgaaacaggc aaagcctatg ccgcaccttt aacagaaagc gcgcaggaat tcagcgacaa
      361 agtatttgcc acattcggcg acaagcgcac aactcggctg tttgccgatt tggatgcact
      421 ggctgaagtg atggaaaaaa caatctcgga aaataaaaaa taggggggca aatatgtgga
      481 aaatgttgaa acacatagcc caaacccacc gcaagcgatt gattggcaca ttttccctgg
      541 tcggactgga aaaccttttg atgctggtgt atccggtgtt tggcggccgg gcgatcaatg
      601 ccgtgattgc gggggaggtg tggcaggcgt tgctgtacgc tttggttgtg cttttgatgt
      661 ggctggtcgg tgcggtgcgg cggattgccg atacgcgcac gtttacgcgg atttataccg
      721 aaatcgccgt gccggtcgtg ttggaacagc ggcagcgaca agtcccgcat tcggcggtaa
      781 ctgcgcgggt tgccctgtcg cgtgagtttg tcagcttttt tgaagaacac ctgccgattg
      841 ccgcgacatc cgtcgtatcc atattcggcg cgtgcatcat gctgctggtg ctggaatttt
      901 gggtcggcgt gtcggcggtg ggcatacttg cgttgttttt atggcttttg ccacgttttg
      961 ccgccatcag cgaaaacctg tatttccgcc tgaacaacag cttggaacgc gacaaccact
     1021 ttatccgaaa aggcgaccgg cggcagctgt accgccatta cggactgctt gcgcgcctgc
     1081 gtgtgctgat ttccaaccgc gaagccttcg gctatctctg cgtcggcacg gcgatgggta
     1141 ttttgttcgg ctttgctttt gtgatgatga cgctcaaagg ctacagcagc gcggggcatg
     1201 tctattcggt cggcacttat ctgtggatgt ttgccatgag tttggacgac gtgccgcgat
     1261 tggtcgaaca atattccaat ttgaaagaca tcggacaacg gatagagtgg tcggaacgga
     1321 acatcaaagc cggaacttga aaaatgccgt ctgaacacgc ttcagacggc atttccatcc
     1381 gttcggcaaa ctacatcaca tccgcccgcc ggttgacaag tttggcaaac aacttttcaa
     1441 cagaagcttc cgcctgcaaa ccaatgcgct ggatcaggct ttgcttctcc tgatatttca
     1501 cttcgataac ctgtttgttt tcaaacgctt tcaacaacaa atcatcactg gtcgaaatct
     1561 cgtcaatcaa gttcaacgcc aacgcctgcc gaccgaacca atgctcgccc gttgccactt
     1621 cctcaatatc caattgaggg cggttctcgc tgacaaactg cttgaacaac tgatgcgttt
     1681 cctccagttc ctgtcggaat ttctgtttgc ccttttccgt attttcaccc ataaaagtaa
     1741 ccgtgcgctt aaattcgccc gccgtcatca catccacatc aatatcatgt tttttcaaca
     1801 ggcggtggat attcggtact tccgccacca cacccaccga accgacaatc gcaaacggag
     1861 cggaagcaat tttatccgcc acacacgcca tcatataacc gccgctcgcc gccaccttat
     1921 cgacggcgac ggtcagcgga atattgcgtt cgcgcaaacg cctaagctgc gaagccgcca
     1981 aaccgtaacc gtgaaccacg ccgcccggac tttccaatct gagcagaacc tcatcttcag
     2041 gcttggcaat caaaagcacc gccgtaatct catgacgcaa ggattctacg gcgtgtgcat
     2101 acaaatcgcc gtcaaaatcc aacacaaaaa ggcgggattt ttgcgtttcg gcagatttct
     2161 ccccaccctc cttcaaacgc tttttctctg ctttggcttc cgccttttcc tttttctttt
     2221 cctctttttc ctgatgtttt gcctcttccc cgcttaaaaa gaatgcttca aacgattgcc
     2281 gctgtttttt ataattttcc gaaaaatccg tcagtacgac actgccgctt tccgactgtt
     2341 tcttactctg tacgatagcc aacacaatca gcgcaattgc gccgaacacg gtaagcagtt
     2401 cgagcaggaa aataccgtaa ttcagtaaaa tttctttcca cattgattgg atttcctctt
     2461 gttcaggcat gaacatgtca atattgtcca tcaccgtccg acagataaaa aaataaccgc
     2521 ttggagcggc attgtcattt tcagcttggt gcccggagcc ggaatcgaac cggcacggga
     2581 tgtttagtcc cgacggattt taagtccgtt gtgtctacct atttcaccac ccgggcattt
     2641 gtgaaaggtg gaggcggggg cgcggatttt aaccggcctg tatgaagatt gcactcctca
     2701 tagcataaac actctgccac cccgccatag tacgataatg gaggcgagag tcggaatcga
     2761 accggcgtag acggatttgc aatccgctgc ataaccactt tgctatctcg ccctaaaact
     2821 ggcttatcta
//
LOCUS       DAWGRZ010000202         2732 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.202, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000202 DAWGRZ010000000
VERSION     DAWGRZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.202"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            89..412
                     /locus_tag="WHT44_001912"
     CDS             89..412
                     /locus_tag="WHT44_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ2534041.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFHLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            526..2703
                     /locus_tag="WHT44_001913"
     CDS             526..2703
                     /locus_tag="WHT44_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ2534042.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRLTRKPLFEVRAEAGNRKHFGLDADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRRRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYTRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          659 a          979 c          686 g          408 t
ORIGIN      
        1 cgttacaact ccgcccactg aaagcagcct gcaacgaagc caaaacgaca aaccgcatcg
       61 taaaccaccc aacccatagg agaaccccat gcaaaacgaa accatcaacc tgaaacagca
      121 ccttgccgcc atcaaagaat actggcagcc cgaaatcatc aaccgccacg ggttccaatt
      181 ccacttggtc aaacttttgg gcgattacgg ctggcatacg cacggataca gcgacaaagt
      241 gctgtttgcc gtggagggcg acatggcggt ggacttcgcc gacggcggca gcatgacgat
      301 acgcgagggc gagatggcgg tcgtgccgaa gtcggtgtcg caccgcccgc gttcggaaaa
      361 cggctgctcg ttggtgctga ttgagttgtc cgacccgtcc gaggccgtct gaaaacgaag
      421 tttccgaagg aagctgagtt tctgcgaagc taaaagcagc ctgcaccttc aatcaatatg
      481 ccgaaaatac aaccccaccg cacaccaaca cacaaaggaa atcccatgac acgcttcaaa
      541 tattccctgc tgtttgccgc cctgttgccc gtgtacgcgc aggccgatgt ttctgtttca
      601 gacgacccca aaccgcagga aagcactgaa ttgccgacca tcaccgttac cgccgaccgc
      661 accgcgagtt ccaacgacgg ctacactgtt tccggcacgc acaccccgct cgggctgccc
      721 atgaccctgc gcgaaatccc gcagagcgtc agcgtcatca catcgcaaca aatgcgcgac
      781 caaaacatca aaacgctcga ccgcgccctg ttgcaggcga ccggcaccag ccgccagatt
      841 tacggctccg accgcgcggg ctacaactac ctgttcgcgc gcggcagccg catcgccaac
      901 taccaaatca acggcatccc cgttgccgac gcgctggccg atacgggcaa tgccaacacc
      961 gccgcctatg agcgcgtaga agtcgtgcgc ggcgtggcgg ggctgctgga cggcacgggc
     1021 gagccttccg ccaccgtcaa tctggtgcgc aaacgcctga cccgcaagcc attgtttgaa
     1081 gtccgcgccg aagcgggcaa ccgcaaacat ttcgggctgg acgcggacgt atcgggcagc
     1141 ctgaacaccg aaggcacgct gcgcggccgc ctggtttcca ccttcggacg cggcgactcg
     1201 tggcggcggc gcgaacgcag ccgcgatgcc gaactctacg gcattttgga atacgacatc
     1261 gcaccgcaaa cccgcgtcca cgcaggcatg gactaccagc aggcgaaaga aaccgccgac
     1321 gcgccgctca gctacgccgt gtacgacagc caaggttatg ccaccgcctt cggcccgaaa
     1381 gacaaccccg ccacaaattg ggcgaacagc cgccaccgtg cgctcaacct gttcgccggc
     1441 atcgaacacc gcttcaacca agactggaaa ctcaaagccg aatacgacta cacccgcagc
     1501 cgcttccgcc agccctacgg cgtagcaggc gtgctttcca tcgaccacaa caccgccgcc
     1561 accgacctga ttcccggtta ttggcacgcc gacccgcgca cccacagcgc cagcgtgtca
     1621 ttgatcggca aataccgcct gttcggccgc gaacacgatt taatcgcggg tatcaacggt
     1681 tacaaatacg ccagcaacaa atacggcgaa cgcagcatca tccccaacgc cattcccaac
     1741 gcctacgaat tttcccgcac gggtgcctac ccgcagcctg catcgtttgc ccaaaccatc
     1801 ccgcaatacg gcaccaggcg gcaaatcggc ggctatctcg ccacccgttt ccgcgccgcc
     1861 gacaaccttt cgctgatttt gggcggacga tacacccgtt accgcaccgg cagctacgac
     1921 agccgcacac aaggcatgac ctatgtgtcc gccaaccgtt tcacccccta cacaggcatc
     1981 gtgttcgacc tgaccggcaa cctgtctctt tacggctcgt acagcagcct gttcgtcccg
     2041 caatcgcaaa aagacgaaca cggcagctac ctgaaacccg taaccggcaa caatctggaa
     2101 gccggcatca aaggcgaatg gcttgaaggc cgtctgaacg catccgccgc cgtgtaccgc
     2161 gcccgtaaaa acaacctcgc caccgcagca ggacgcgacc cgagcggcaa cacctactac
     2221 cgcgccgcca accaagccaa aacccacggc tgggaaatcg aagtcggcgg ccgcatcacg
     2281 cccgaatggc agatacaggc aggttacagc caaagcaaaa cccgcgacca agacggcagc
     2341 cgcctgaacc ccgacagcgt acccgaacgc agcttcaaac tcttcactgc ctaccacttt
     2401 gcccccgaag cccccagcgg ctggaccatc ggcgcaggcg tgcgctggca gagcgaaacc
     2461 cacaccgacc ctgccacgct ccgcatcccc aaccccgccg ccaaagcccg cgccgccgac
     2521 aacagccgcc aaaaagccta cgccgtcgcc gacatcatgg cgcgttaccg cttcaatccg
     2581 cgcgccgaac tgtcgctgaa cgtggacaat ctgttcaaca aacactaccg cacccagccc
     2641 gaccgccaca gctacggcgc actgcggaca gtgaacgcgg cgtttaccta tcggtttaaa
     2701 taaggtcgtc tgaaaacgga gtttctgcga ag
//
LOCUS       DAWGRZ010000203         2703 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.203, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000203 DAWGRZ010000000
VERSION     DAWGRZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.203"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..732
                     /locus_tag="WHT44_001914"
     CDS             <1..732
                     /locus_tag="WHT44_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ2534043.1"
                     /translation="YIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKD
                     GNRLARHHEHGKIWKKPTRHEDIADFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWA
                     EDYQFWYDVSKLGRLAYYPEALVKYRLHANQVSSKYSIRQHEIAQGIQKTARNDFLQS
                     MGFKTRFDSLEYRQIKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTLPAGAWLDFA
                     ADGRMRRLFTLRQYFGILHRLLKNR"
     gene            774..1601
                     /locus_tag="WHT44_001915"
     CDS             774..1601
                     /locus_tag="WHT44_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ2534044.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYITVFEDDVLLGEGAEKFLA
                     EDAWLQERFDPDTAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISR
                     KAMRFFLDRFAALPPEGLHPVDLMMFSDFFDREGMPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQRRDSPANTFKHRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            1613..1708
                     /locus_tag="WHT44_001916"
     CDS             1613..1708
                     /locus_tag="WHT44_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ2534045.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILRRLLKNR"
     gene            1750..2592
                     /locus_tag="WHT44_001917"
     CDS             1750..2592
                     /locus_tag="WHT44_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ2534046.1"
                     /translation="MQNHVISLASAAERRAHIAATFGVRGIPFQFFDALMPSEELNRM
                     MAELVPGLAKQHLLSEVEKACFMSHAVLWKQALDEGLPYVAVFEDDVLLGKDAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT          702 a          639 c          733 g          629 t
ORIGIN      
        1 tatattgcgc gcaccgatgc agacgatatt gccgcccccg actggattga gaaaatcgtg
       61 ggtgagatgg aaaaagaccg cagcatcatc gcgatgggtg cgtggctgga agttttgtcg
      121 gaagaaaagg acggcaaccg gctggcgcgg caccacgaac acggcaaaat ttggaaaaaa
      181 ccgacccgac acgaagacat tgccgacttt ttccctttcg gcaaccccat acacaacaac
      241 acgatgatta tgaggcgcag cgtcattgac ggcggtttgc gttacaacac cgagcgggat
      301 tgggcggaag attaccaatt ttggtacgat gtcagcaaat tgggcaggct ggcttattat
      361 cccgaagcct tggtcaaata ccgccttcac gccaatcagg tttcatccaa atacagcatc
      421 cgccaacacg aaatcgcgca aggcatccaa aaaaccgcca gaaacgattt tttgcagtct
      481 atgggtttta aaacccggtt cgacagcctt gaataccgcc aaataaaagc agtagcgtat
      541 gaattgctgg agaaacattt gccggaagaa gattttgaac gcgcccgccg gtttttgtac
      601 caatgcttca aacggacgga cacgctgccc gccggcgcgt ggctggattt tgcggcagac
      661 ggcaggatgc ggcggctgtt taccttgagg caatacttcg gcattttgca ccgattgctg
      721 aaaaaccgtt gaaaaacgcc gctttatcca acagacaaaa aacaggataa attatgcaaa
      781 accacgttat cagcttagct tccgccgcag aacgcagggc gcacattgcc gataccttcg
      841 gcaggcacgg catcccgttt cagtttttcg acgcactgat gccgtctgaa aggctggaac
      901 aggcaatggc ggaactcgtc cccggcttgt cggcgcaccc ctatttgagc ggagtggaaa
      961 aagcctgctt tatgagccac gccgtattgt ggaagcaggc attggacgaa ggtctgccgt
     1021 atatcaccgt atttgaggac gacgttttac tcggcgaagg tgcggaaaaa ttccttgccg
     1081 aagacgcttg gctgcaagaa cgctttgacc cggataccgc ctttatcgtc cgcttggaaa
     1141 cgatgtttat gcacgtcctg acctcgccct ccggcgtggc ggattactgc gggcgcgcct
     1201 ttccgctgtt ggaaagcgaa cactggggga cggcgggcta tatcatttcc cgaaaagcga
     1261 tgcggttttt cctggacagg tttgccgccc tgccgcccga agggctgcac cccgtcgatc
     1321 tgatgatgtt cagcgatttt ttcgacaggg aaggaatgcc ggtttgccag ctcaatcccg
     1381 ccttgtgcgc ccaagagctg cattatgcca agtttcacga ccaaaacagc gcattgggca
     1441 gcctgatcga acacgaccgc cttctgaacc gcaaacagca aaggcgcgat tcccccgcca
     1501 acacattcaa acaccgcctg atccgcgcct tgaccaaaat cagcagggaa agggaaaaac
     1561 gccggcaaag gcgcgaacag ttcattgtgc ctttccaata aaaggaggag agatggacat
     1621 cgtatttgca gcagacggca ggatgcggcg gctgtttacc ttgaggcaat acttcggcat
     1681 tttgcgccga ttgctgaaaa accgttgaaa aacgccgctt tatccaacag acaaaaaaca
     1741 ggataaatta tgcaaaacca tgttatcagc ttagcttccg ccgcagaacg cagggcgcac
     1801 attgccgcaa ccttcggcgt gcgcggcatc ccgtttcagt ttttcgacgc actgatgccg
     1861 tctgaagaac tgaatcggat gatggcggaa ttggtgccgg gattggcaaa acagcacctg
     1921 ttgagcgaag tggaaaaagc ctgctttatg agccacgccg tattgtggaa acaggcattg
     1981 gatgaaggtc tgccctatgt tgccgtattt gaggatgatg tcctgcttgg caaagacgca
     2041 gaaaagttcc ttgccgaaga tacttggttg gaagagcgtt ttgataagga ttctgccttt
     2101 atcgttcgtt tggaaacgat gtttgcgaaa gttattgtca gaccggataa agtcctgaat
     2161 tatgagaatc ggtcatttcc tttgctggag agcgaacatt gggggacggc tggctatatc
     2221 atttcgcgtg aggcgatgcg gtttttcttg gaaaggtttg ccgttttgcc ggcagagtgg
     2281 attaaagcgg tagattggat gatgtttact tatttctttg ataaggaggg gatgcctgtt
     2341 tatcaggtta atcccgcctt atgtacccaa gaattgcatt atgccaagtt cctcagtaaa
     2401 aacagtatgt tgggtagcga tttggaaaaa gatagggaac aagaaagaag acaccgccgt
     2461 tcgttgaagg tgatgtttga cttgaagcgt gctttgggta aattcggtag ggaaaagaag
     2521 aaaagaatgg agcgtcaaag gcaggcggag cttgagaaag cttacggcag gcgggtcata
     2581 tcgttcaaat agtttgtgta aaatatagcg gattaaaatt agaaatggat acactgtcat
     2641 tcccgcgcag gcgggaatct agtccgttcg gtttcgcttg ttttaagttt cgggtaactt
     2701 cca
//
LOCUS       DAWGRZ010000204         2580 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.204, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000204 DAWGRZ010000000
VERSION     DAWGRZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.204"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(195..266)
                     /locus_tag="WHT44_001918"
     tRNA            complement(195..266)
                     /locus_tag="WHT44_001918"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(231..233),aa:Arg,seq:cct)
     gene            complement(286..733)
                     /locus_tag="WHT44_001919"
                     /pseudo
     CDS             complement(286..733)
                     /locus_tag="WHT44_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
     gene            complement(738..1712)
                     /gene="ispB"
                     /locus_tag="WHT44_001920"
     CDS             complement(738..1712)
                     /gene="ispB"
                     /locus_tag="WHT44_001920"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ2534047.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(1728..1916)
                     /locus_tag="WHT44_001921"
     CDS             complement(1728..1916)
                     /locus_tag="WHT44_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534048.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            1936..2244
                     /gene="rplU"
                     /locus_tag="WHT44_001922"
     CDS             1936..2244
                     /gene="rplU"
                     /locus_tag="WHT44_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ2534049.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2269..2541
                     /gene="rpmA"
                     /locus_tag="WHT44_001923"
     CDS             2269..2541
                     /gene="rpmA"
                     /locus_tag="WHT44_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ2534050.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
BASE COUNT          756 a          693 c          575 g          556 t
ORIGIN      
        1 ataaaacaac agatttccaa gccggaacat cttttacgaa gcctgaaaat catttcatga
       61 ttctaccgtc ctaaaggtcg gtttttcaag caggaagaaa aagttttcag atggcaaaaa
      121 agccctccaa cactgaaagg ccttatatcg gaaacttccc gcaacacggg aaacagacaa
      181 atgaaatcgt caaacctcgc caacaggaat cgaacctgta ttttacgctt aggaggcata
      241 cgttctatcc gttgaactat ggcgagccga aatgaaaagg agattttaac cctttccgac
      301 gacaaaagac aagatgccgg ccgcaatcag cggcccgaca gggataccgc ccataaatgc
      361 caccccgata accgtcccga ttaacagccc tgtaattaaa acaggctgct gtcccatcat
      421 aaggcacgcc gcgtcccgcc agccaagcca cgaaaatacc gataaaaacg gcggatatca
      481 ttttaaaatt caaaaattcg gcaacgggag gaacctgcgc ctttcctgaa accaacggac
      541 tcaaaacccc tatggtcaaa agaatgatac cgagattcaa cccgtgcttc tcgaccaacg
      601 ggacaaactg tatcaatgcc gtctgctgca tcagcagcaa tatggttgcc gagatggtaa
      661 tcgaattgtt gttgctgacc acccccaaca gaatcagcgt aaccagaaac aggggaacaa
      721 aactgaaatt catcgcctca agacaccctg accaaagatt ccttcgccag ctgaatcatg
      781 gcatccttca cttcgctgtc gggcagggca tccaaggcgg taacggcaca atcgactgct
      841 ttgcgcgcct cgcctatcga atatgccaac gcatccgaac ggacgacata atcgtggatt
      901 ttctcaaaat agctgcgatc tgcattttcc aaagcagtac gcacatcgtt cgcaacctgt
      961 tcggaaccct gacgcatcag ataaatcaaa ggcaaagtcg gttttccttc cgccaaatcg
     1021 tcgccgacgt ttttgccggt ttcttcggtt tcgccagaat agtccagcac atcgtcaata
     1081 atttggaatg ccgtaccgac atacataccg tagtctttca acgcccgttc gtgttcgggg
     1141 gaagccttgc ccaaaattgc gccgacttga gcggcagctt caaacaattt tgccgtttta
     1201 tattggatga cttggatata ttgttcttcg gtaatgtccg tattgccgat gttcatcagc
     1261 tgcatgactt cgccctcggc aataatgttg gttgcatccg ccatcacttc caaaacgcgc
     1321 atactgcccg aggcaaccat cagttgaaag gcgcgcgtgt ataaaaagtc gccaaccaac
     1381 acagccgccg cattgccgaa cagattgttt gccgttgccc gcccacggcg caaatcgctt
     1441 tcatcgacga catcgtcgtg caggagggtg gaagtgtgga taaactcgac catcgccgcc
     1501 agcgaataca gtttctcgtc atcataaccg accgccttac ccgccaaaat cgtcataatc
     1561 ggacgcaggc gtttgccgcc cgcgctgatg atatatgtac cgatttgcga aatcagtgcg
     1621 acatcggatt gcaccgcacg gttgatgact tcattgactt tggcaaggtc ttcaggcaga
     1681 tgtcgctgga aatagggcag attctcgagc atggcagact ctcaggttta cgtttccgtc
     1741 aaacggcaga taccgcttga aagctgattc aaatagcggg caattatatc agcaaagcca
     1801 tcagggtaca tcttttcaga cggcatatgg ttatccgaac cgcgcaaact ttgacaaaac
     1861 aggcaaataa caatacaatg ccgagttccg cacaatggtg tgcggatatt aaccataacc
     1921 cttatggagt tgagtatgta cgcggtcgta aaaaccggcg gcaaacagta taaagtttcc
     1981 gtcggcgaaa aattgaaagt agaacagata ccagccgaac tcgacagcca aatcgaactg
     2041 accgaagttt tgatgattgc tgacggcgaa tctgtaaaag ttggcgcacc ctttatcgaa
     2101 ggtgcaaaag taacggctaa agtagtggca cacggtcgtg gcgaaaaagt ccgcatcttc
     2161 aaaatgcgcc gccgcaaaca ctaccaaaaa cgccaaggcc accgccaaaa tttcacccaa
     2221 atcgaaatcg tggcaatcgc ctaaccttaa aacttaggag cattacaaat ggcaagtaaa
     2281 aaagcaggcg gcagcacccg caacggtcgc gattcagaag ccaaacgctt gggcgttaaa
     2341 gcctacggca acgagctgat tcccgcaggt tccatcatcg tacgccaacg cggtaccaaa
     2401 ttccacgcag gcgacaacgt aggtatgggc aaagaccaca ctttgttcgc caaagttgac
     2461 ggttatgtcg aattcaaaac caaaggcgcg ctgaaccgta aaactgtcag catccgtcct
     2521 tacaccggtt ctgaagaata atccaaccga tacctgaagc cgcatctttt cacgatgcgg
//
LOCUS       DAWGRZ010000205         2580 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.205, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000205 DAWGRZ010000000
VERSION     DAWGRZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.205"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            73..687
                     /locus_tag="WHT44_001924"
     CDS             73..687
                     /locus_tag="WHT44_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ2534051.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPILLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGIYILVENNSFDMLWTVLG"
     gene            848..2509
                     /locus_tag="WHT44_001925"
     CDS             848..2509
                     /locus_tag="WHT44_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ2534052.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPDE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADSYDAARFA
                     GVFFAVIGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPVAHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDYHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIGIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVLLPQNADAPQGWQTVWQGARPRNKDSKFALIRKIGENI"
BASE COUNT          505 a          650 c          668 g          757 t
ORIGIN      
        1 cggggttgaa gttgacatct acaaggttta atgtttgaat acatttaaaa atatatttga
       61 atgagatgtt ttatgattca aaatgtcgtt acttcaataa tcctgtattc tgggacagcc
      121 gtagacttac ttattatcct aatgttattt tttgccaaaa gaaaaagtag aaaagacatc
      181 attaacatct atttaggaca atttctaggc tctgttagtc tgatattgct aagtttactt
      241 tttgcatttg tcttagatta tattcctagt aaagagattt taggtttgct cggcttgatt
      301 ccaattctcc taggcatcaa agttttgctt ttaggagatt ctgatggaga ggctattgcc
      361 aaagagggtt tgcgcaaaga taataaaaac ctgatttttc tagtcgctat gattactttt
      421 gcaagttgtg gtgctgacaa tattggtgtc tttgtcccat attttactac cttaaattta
      481 gcgaatttga tagtggcttt acttaccttt ctagtcatga tttatctctt ggttttttct
      541 gcccaaaaat tagcacaagt cccttctgtt ggagaaactt tggaaaaata tagcagatgg
      601 tttgttgctg ttgtttattt aggattgggg atatatatcc tggttgaaaa caacagtttt
      661 gatatgctat ggactgtgtt gggctaggaa aaaatattat gaaaaagata gtatctgcaa
      721 agactgccat gtgcagtctt ttttgttgcc ggtctttttg tgtctgatgc cgtctgaagc
      781 agtctctgca cgacctttgt gcgaatattt gctacacttg gcaatccgtc cattttttct
      841 cacgcttatg ctgacctata ccccgcccga tgcccgcccg cccgccaaaa cccacgaaaa
      901 gccgtggctg ctgctgttga tggcgtttgc ctggttgtgg cccggcgtgt tttcccacga
      961 tttgtggaat cctgacgaac ctgccgtcta taccgccgtc gaagcactgg caggcagccc
     1021 cacccccttg gttgcccatc tgttcggtca aaccgatttc ggcataccgc ccgtgtatct
     1081 ttgggttgcc gccgcgttca aacatttgct gtcgccgtgg gctgccgact catacgatgc
     1141 cgcacgcttt gcaggcgtat tttttgccgt tatcggactg acttcctgcg gctttgccgg
     1201 tttcaacttt ttgggcagac accacgggcg cagcgtcgtc ctgattctca tcggctgtat
     1261 cgggctgatt ccagttgccc atttcctcaa ccccgctgcc gccgcctttg ccgccgccgg
     1321 actggtgctg cacggttatt ctttggctcg ccggcgcgtg attgccgcct cttttctgct
     1381 cggtacgggc tggacgctga tgtcgttggc agcagcttat ccggcagcat ttgccctgat
     1441 gctgcccttg cccgtactga tgtttttccg tccgtggcaa agcaggcgtt tgatgttgac
     1501 ggcagtcgcc tcacttgcct ttgccctgcc gcttatgacc gtttacccgc tgctcttggc
     1561 aaaaacgcag cccgcgctgt tcgcgcaatg gctcgactat cacgttttcg gtacgttcgg
     1621 cggcgtgcgg cacgttcaga cggcattcag tttgttttac tatctgaaaa acctgctttg
     1681 gtttgcattg cccgcgctgc cgctggcggt ttggacggtt tgccgcacgc gcctgttttc
     1741 gaccgactgg gggattttgg gcgtcgtctg gatgcttgcc gttttggtgc tgcttgccgt
     1801 caatccgcag cgttttcagg ataacctcgt ctggctgctt ccgccgcttg ccctgttcgg
     1861 cgcggcgcaa ctggacagcc tgaggcgcgg cgcggcggcg tttgtcaact ggttcggcat
     1921 tatggcgttc ggactgtttg ccgtgttcct gtggacgggc tttttcgcca tgaattacgg
     1981 ctggcccgcc aagcttgccg aacgcgccgc ctatttcagc ccgtattatg ttcctgatat
     2041 cgatcccatt ccgatggcgg ttgccgtact gttcacaccc ttgtggctgt gggcgattac
     2101 ccggaaaaac atacgcggca ggcaggcggt taccaactgg gcggcaggcg ttaccctgac
     2161 ctgggctttg ctgatgacgc tgttcctgcc gtggctggac gcggcgaaaa gccacgcgcc
     2221 ggtcgtccgg agtatggagg catcgctttc cccggaattg aaacgggagc tttcagacgg
     2281 catcgagtgt atcggcatag gcggcggcga cctgcacacg cggattgttt ggacgcagta
     2341 cggcacattg ccgcaccgcg tcggcgatgt acaatgccgc taccgcatcg tcctcctgcc
     2401 ccaaaatgcg gatgcgccgc aaggctggca gacggtttgg cagggtgcgc gtccgcgcaa
     2461 caaagacagt aagttcgcac tgatacggaa aatcggggaa aatatataaa aaacaacaga
     2521 ttgagccgaa tttctggatt aagtgccgga aatcgcgtat aattgcgcga ttaaaccttt
//
LOCUS       DAWGRZ010000206         2565 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.206, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000206 DAWGRZ010000000
VERSION     DAWGRZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.206"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(12..182)
                     /locus_tag="WHT44_001926"
     CDS             complement(12..182)
                     /locus_tag="WHT44_001926"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534053.1"
                     /translation="MAIMAYFGGFVRALFDLGVFERGGVKGRGKWFSRVWRRSEVLCL
                     MCGGIFSVKLIL"
     gene            242..2467
                     /locus_tag="WHT44_001927"
     CDS             242..2467
                     /locus_tag="WHT44_001927"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ2534054.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTSDIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVIVPEATSVSIVFTDKQGNKKELREPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGDKLAAAGVNVNNGFGNLLANLDKLDADTRTAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT          710 a          782 c          589 g          484 t
ORIGIN      
        1 aatttaaacc actataaaat caatttcacc gaaaatatgc cgccgcacat caggcacagc
       61 acctccgacc gccgccaaac gcgggaaaac catttccccc ttcctttcac gccgccgcgt
      121 tcaaacacgc caagatcgaa caaagcgcga acaaagccgc caaaatatgc cataatagcc
      181 acgcacattc cccgcgcaat gtgttccaaa agaaaaaacc aacctacaag agaataatcc
      241 tatgactcaa aaatccacca ttgtttatac ccataccgac gaagcacccg cgctggcgac
      301 ccaatcgctg ctgccgattg tgcaggcttt tgcccgccac gccgatattg atgtcaaaac
      361 cagcgacatt tctctctccg gccgtatttt ggcggcgttt cccgaatacc tgaccgaagc
      421 gcaacgcgta cctgatgcgc ttgccgaatt gggcgaactg gtgaaacaac ccgatgcaaa
      481 cgtaatcaaa ctgccgaaca tcagcgcgtc cgtaccgcaa ctgactgccg cgattaaaga
      541 attgcaatct aaaggctttg ccgttcccga ctatcccgcc gacccgcaaa ccgatgaaga
      601 aaaagccgta cgcgaacgct acgaccgcat caaaggcagc gcggtaaacc ctgtcctgcg
      661 tgaaggcaac tccgaccgtc gcgcccctaa agcagtgaaa aactttgcga aaaaaaatcc
      721 gcacagcatg ggcgcatgga ccaaagactc caaaacccac gttgccacca tgcaaagcgg
      781 cgactttttt cataacgaac aatctgttat cgtacctgaa gcgacttccg tatccatcgt
      841 attcaccgac aaacaaggca acaaaaaaga gctgcgcgag cccgtcgccc tgaaagccgg
      901 cgaaatcatc gacgcgaccg tgatgagtaa aaaagccctg ctcgccttcc ttgccgaaca
      961 agtaaaagat gcgaaagcaa aaggcgtgtt gttctcgctg cacatgaaag ccactatgat
     1021 gaaagtgtcc gacccgatta tcttcggaca cgccgtcaaa gtattcttcg cgcctgtttt
     1081 tgaaaaattc ggcgacaaac tggctgctgc cggcgtcaac gttaacaacg gcttcggcaa
     1141 cctgcttgcc aatctggaca aactggatgc ggacacccgc accgccgtcg aagccgaaat
     1201 cgccgccgtt tacgctgcca accccgattt ggcgatggtt gattccgaca aaggcatcac
     1261 caacctgcac gttcccagcg atgtcatcgt cgatgcctct atgcctgcga tgattcgcaa
     1321 ttccggccgt atgtgggaca aaaacggcaa agcgcaagac accaaagccg tgattcccga
     1381 cagcagctat gccggcgttt accaagcaac catcgacttc tgccgcgaac acggcgcatt
     1441 tgacccgaca accatgggta ctgtgcccaa cgtcggactg atggcgcaag cggcggaaga
     1501 atacggctcg cacaacaaaa cctttgaaat cgaagccgac ggccaagtcc aagtcattga
     1561 tgcggcagga aaagtcctaa tgcagcacga cgttgaagcc ggcgacatct ggcgtatgtg
     1621 ccaaaccaaa gacgctccgg ttaaagactg ggtacaactt gccgtcaacc gcgcccgtct
     1681 gagcaacacg cctgccgtgt tctggctcga cgaaaaccgt ccgcacgaca agagcctgct
     1741 cgccaaggtt aaagcctacc ttgccgaact ggataccaat ggcctcgaca tccgcgtcct
     1801 cgctcctgaa gaagccgcca agttcagctt gggtcgtctg aaaaacggcg aagacaccat
     1861 ctccgtaacc ggtaatgtct tgcgcgacta cctgaccgac ttgttcccaa ttttggaact
     1921 cggcacaagc gcgaaaatgc tgtctatcgt tccattgatg aacggcggcg gtatgtttga
     1981 aaccggcgcg ggcggttctg caccgaaaca tgttcaacaa ttcctcgaag aaaaccattt
     2041 gcgctgggac tcgctgggcg aattcctcgc actcgccgta tcgtttgaac atttggcgca
     2101 aaaaaccggc aatgccaaag cccaagtcct cgccgacact ttggatgcag ccaccgaaaa
     2161 actgctgttg aacgacaaat cgcctaaacg taaagccggc gaactcgaca accgcggcag
     2221 ccatttctac ctcaccctct actgggcgca agaattggcg gcgcaagaca aagatgccga
     2281 actgaaagcc gcatttacgc cattggcagc cgctttgacc gccgacgaag cgaaaatcgt
     2341 tgccgagctt tccgccgtac aaggcaaagc ggccgacatc ggcggctact acgccgccaa
     2401 tcctgaaaaa gccgcacaag tgatgcgtcc gagcgtaacc tttaatcagg cactaaacgc
     2461 cttataagca caaaggtaaa aatgccgtct gaacattcgt cgttcagacg gcattagtta
     2521 tccctatctg cctgattatg attaaacgcc gcacccattg gatat
//
LOCUS       DAWGRZ010000207         2547 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.207, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000207 DAWGRZ010000000
VERSION     DAWGRZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.207"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            11..1353
                     /locus_tag="WHT44_001928"
                     /pseudo
     CDS             11..1353
                     /locus_tag="WHT44_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            1398..1688
                     /locus_tag="WHT44_001929"
     CDS             1398..1688
                     /locus_tag="WHT44_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534055.1"
                     /translation="MNIQLQGHIVGVKKINGQIEGKSFDYCCLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            1693..1890
                     /locus_tag="WHT44_001930"
     CDS             1693..1890
                     /locus_tag="WHT44_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534056.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            1898..2182
                     /locus_tag="WHT44_001931"
     CDS             1898..2182
                     /locus_tag="WHT44_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534057.1"
                     /translation="MFGGRLKNAPSITAKHFLKENIMKFINTCRKYGAKLAVVTAAPL
                     ALAAHANATLPDTAKNALEAAKADGMEAGWIVVGIFAALFVFSIVKRVMK"
     gene            2189..2467
                     /locus_tag="WHT44_001932"
     CDS             2189..2467
                     /locus_tag="WHT44_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534058.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
BASE COUNT          755 a          586 c          599 g          607 t
ORIGIN      
        1 gattcgcacc atggaagata agcaagggat gacaaaggcg gttgccggcg tgatgacgga
       61 cgcgctagcg gacggcagga agccgacaac cgcttcaaat cttcccccct tatctaacag
      121 ggggggacag aaaccgaaac ggcaggcagg gttcaggaag tcttcgaatg ttacgaaacg
      181 tacataacgg acggtaaagg aaacctgtta ggcgttcctc ttcggcgcgg tgtatcagat
      241 tcggctttca ttgatcaaat tagcttttca tttcatgaaa aaaccttttt cgataaatac
      301 ggcgttcgtg taagtctttt ggaagacgaa gattttattc gcgccgcgtc catgctcgcc
      361 gaagaagttt tcggtttcgg tatctacaaa gaatccaaag gttcgggcgg tcgtttctat
      421 gagcgctgtt ggttgatggg ttcggaagac gccctatacg gtcgcgtcca ttttggcggc
      481 caacaaaata ccattctttt cgaactgacc ggcaccggtt gcggcgtcgc aaaagaaggc
      541 tgggaatcac gacttttcgc attcctgact aatgcaatcc gcccaaaaat cacacgcgtt
      601 gacatcgcaa aagacttttt caacggcgaa tacagcccga accaagcccg tgaagaccga
      661 aataaaggta tgtttacctg tcatcacgtc aaaccaaaag gcgaatgttt ggggtcagat
      721 tgggaagaag acgatgaagc caaaatgacc aaaggcaaga cctatggtat cggctcccgt
      781 gaatcgtcca aatatgtccg cgtctatgaa aaaggcaagc agttgggcga taaaacaagc
      841 acatggacgc gatttgaaat tgaattcaaa gcaaaagaca tcgttatccc tttcgaagtt
      901 ttgcagaatc cgggcgaata tttcggcggc gcatatccga tttgcgaacg attcgcccaa
      961 aaggcaacgc gcatacacgc ggttaaggaa gataaggtca tttcagccga ccgctacctt
     1021 gaatgggtaa aaaaacagtt cggacgtgcg gcaaacggtc tgaaattcat ttttcccgaa
     1081 ttggacaaag ccaaactgtt tgaactgatt gagccgagtc atcacaagct gcccaagtct
     1141 ttggctcccg aagcctacga ctgcgccttt ttgaaagctc aagccattca tgaacagccc
     1201 gcattcaaac cgtacaaaga cccttactat atgtacgaat attacgagaa tcttgaaaaa
     1261 cagcttgaac agcaaaaaca cgtcaacaat gaagaaagct ataacaactt catttacgac
     1321 aaattcgcaa gactaccgat ttcatgggct taaagtgtct gcccgaaaga cgtttaatca
     1381 cacaaggaaa ccaaaaaatg aacatccaac ttcaaggcca catcgtcggc gttaaaaaaa
     1441 tcaacggaca aatcgaaggc aagagcttcg actattgctg cctgattgtc gccacaccct
     1501 tagacagctc ccaaggcaac gcattgggca gctctactac tgaatacgat ttcggcggct
     1561 ctgccaattt cgagcagttc cgaaacgccc aatttccgat cgaagcaaac ctgaacgtag
     1621 aaatcgtcac tacgggcaaa acccaaaaac tgaaagtcat cggttttcaa ctcgtgaaga
     1681 aaggctgatt gaatgcagaa agtctatgtt gtccagtccg tatcaacagg ggactttctg
     1741 tatctctctc ctgaaacggg cgacatcgga cataccaaat taatcaccaa tgccgattat
     1801 ttctacgact tcgaagaagc gattaacgca ggtttggaag aaatcggcaa ccaatacgaa
     1861 tttgtcgtat tcggattttt gaaagactga ttttcggatg ttcggcggtc gtctgaaaaa
     1921 cgctccatcc attaccgcca aacacttttt gaaggaaaat atcatgaaat ttattaacac
     1981 ctgccgtaaa tacggcgcaa aactggctgt tgtaacagct gctcccctgg ctttggccgc
     2041 acatgcaaat gcaacgttgc ccgatacggc aaaaaacgct ttggaagccg caaaagcgga
     2101 cggtatggaa gccggttgga ttgtagtggg cattttcgcc gcgctttttg tattttccat
     2161 cgttaagaga gtgatgaagt aagacggcat gtactaccaa gtcggaaata aatgtcttga
     2221 gaagcaccag gctgaaaacc tttatttcag cttggtagta ccaagaatca aagaaaacgg
     2281 acagattgtc aggccggaat ataacggcag cctgtggaag atgtcggacg gtcagccgct
     2341 aaggctttta ttggcggaat gcagtccgaa agacaacctg caaagcggtc ttgaaacagg
     2401 ctggatagta ttcggcatcc tcgcgtccgt ttactttgtt tccctgctga aaaaggtttt
     2461 gaaatgatgg atttttattt ttatctcggc gtttccgtac ccgtattaat cggggcggtt
     2521 ctgtttaaga attgagcgca tgaagtt
//
LOCUS       DAWGRZ010000208         2509 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.208, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000208 DAWGRZ010000000
VERSION     DAWGRZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.208"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            167..790
                     /gene="lolA"
                     /locus_tag="WHT44_001933"
     CDS             167..790
                     /gene="lolA"
                     /locus_tag="WHT44_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ2534059.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTKPYRQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1063..2202
                     /locus_tag="WHT44_001934"
     CDS             1063..2202
                     /locus_tag="WHT44_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEZ2534060.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQTPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTQQVKKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESVKLGVKLWQGLKAGK"
     gene            2335..>2509
                     /locus_tag="WHT44_001935"
     CDS             2335..>2509
                     /locus_tag="WHT44_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ2534061.1"
                     /translation="MDLSIVVPIYNVESYLEACLSSIESILSNENVELILVNDGSKDG
                     SEDICYKYIDKISN"
BASE COUNT          687 a          726 c          620 g          476 t
ORIGIN      
        1 gcggcacaca ttaaagggca gcgtggcgcg ccgccttttc cggtgggcaa aaaatcagcc
       61 ctcggaaaac gcggtttgca aaatgcaaac cgcccgtaac gccgcccgta tgattgtttt
      121 gctgcgccga tactttacgc cacactcatc ccgacaagga aaaataatga tgaaaccgca
      181 caacctgttc caattcctcg ccgtttgctc cctgaccgtc gccgtcgctt ccgcacaggc
      241 gggcgcggta gacgcgctta agcaattcaa caacgatgcc gacggtatca gcggcagctt
      301 cacccaaacc gtccaaagca aaaagaaaac ccaaaccgcg cacggcacgt tcaaaatcct
      361 gcgaccgggc cttttcaaat gggaatacac caaaccttac aggcaaacca tcgtcggcga
      421 cggtcaaacc gtttggctct acgatgttga tctggcacaa gtgaccaagt cgtcccaaga
      481 ccaggccata ggcggcagcc ccgccgccat cctgtcgaac aaaaccgccc tcgaaagcag
      541 ctacacgctg aaagaggacg gttcgtccaa cggcatcgat tatgtgctgg caacgcccaa
      601 acgcaacaac gccggctacc aatacatccg catcggcttc aaaggcggca acctcgccgc
      661 catgcagctt aaagacagct tcggcaacca aacctccatc agtttcggcg gtttgaatac
      721 caatccccaa ctctcgcgcg gcgcgttcaa gtttaccccg cccaaaggcg tggacgtgtt
      781 gagcaactga tgccgtccgc cccgatgccg tctgaaagcc gccgaggctt cagacggcat
      841 ttttacgcag gcggaacaat gtcccgcatt accgcccgat cgggcaccgg aacggcaaac
      901 cggtgaaaat taacggttgc gccccggctg tttttgccgt ttaatgcaaa ccttgctgca
      961 ccaagggcca agaaagccga ccggccgccc ccacagcttc cgatgcaggc ggcccgtccg
     1021 tccctgcaat gttttttatt tttgaacgaa aggtcgaaaa ccatgaaaaa aacactggtg
     1081 gcggcggcaa tcctgagcct cgccttgact gcgtgcggcg gcggaagcga taccgccgcc
     1141 caaaccccct ccgccaagcc cgaagccgaa caatcgggca aactcaacat ctacaactgg
     1201 tcggattatg tcgatcccga aaccgttgcc gcctttgaaa aagaaaccgg catcaagacg
     1261 cgttccgatt attacgacag caacgaaaca ctggaggcaa aagtcctgac cggcaaatcc
     1321 ggctacgacc tgaccgcgcc gtccatcgcc aacgtcggcc ggcaaatcaa agcgggcgcg
     1381 tatcagaaaa tcgacaaggc gcaaatcccc cattacggca acatcgataa agatttgctg
     1441 aaaatgatgg aagccgtcga tccgggcaac gaatacgccg tcccctattt ctggggcatc
     1501 aataccttgg caatcaatac ccagcaggtg aaaaaagcat tgggtacgga caagctgccc
     1561 gaaaacgaat gggatttggt gttcaaaccc gaatacaccg ccaaactcaa atcctgcggc
     1621 atcagctatt tcgacagcgc aatcgaacag attcccttgg cgttgcacta tttgggcaaa
     1681 gaccccaaca gtgagaatcc cgaagacatc aaagccgccg tcgatatgat gaaagccgtc
     1741 cggggcgacg tgaaacgctt cagctcttcc ggctatatcg acgatatggc ggcgggcaac
     1801 ctgtgtgccg ccatcggtta cggcggcgat ttgaacattg ccaaaacccg tgccgaagaa
     1861 gccgcaaacg gcgtggaaat caaagtattg accccgaaaa ccggcgtggg cgtgtgggtg
     1921 gattccttta tgattccgcg cgacgcgcaa aacgttgcca atgcccaccg ctatatcgac
     1981 tacacgctcc ggcccgaggt ggcggcgaaa aacggcagct tcgttaccta cgcgcccgcc
     2041 agccgtcccg cgcgcgagct gatggatgaa aaatacacct ccgacgcatc gattttcccg
     2101 aacaaagaac tgatggaaaa aagtttcatc gtatcgccca aatccgcaga atccgtcaaa
     2161 ctgggcgtga agctgtggca agggctcaaa gcgggcaaat aaccggaatc cctgccgtct
     2221 gaaacctttc gggcggcagg aaacggcgcg tccgttatca aacagggggg cgtttcccct
     2281 cctgccggtt atgattgggt taagattaaa atgatttagt aaaatgagaa agatatggat
     2341 ttaagtatcg tagttcctat ttataatgtc gaaagttatt tggaagcgtg tttaagttcc
     2401 atagaatcta tattaagtaa tgaaaatgtc gaacttatcc ttgtgaatga cgggtcaaaa
     2461 gacggaagtg aagatatatg ttacaaatat atagataaaa tatcaaaca
//
LOCUS       DAWGRZ010000209         2487 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.209, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000209 DAWGRZ010000000
VERSION     DAWGRZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.209"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..1032
                     /gene="rfbB"
                     /locus_tag="WHT44_001936"
     CDS             <1..1032
                     /gene="rfbB"
                     /locus_tag="WHT44_001936"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ2534062.1"
                     /translation="IGSAVVRHIIRNTRDAVVNVDKLTYAGNLESLTEVADNPRYAFE
                     QVDICDRAELDRVFAQYRPDAVMHLAAESHVDRSIGSAGEFIQTNIVGTFNLLEAARA
                     YWQQMPSEQHEAFRFHHISTDEVYGDLGGTDDLFTETAPYAPSSPYSASKASSDHLVR
                     AWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGDGMQIRDWLFVE
                     DHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLEELAPEKPAGVARYEDLI
                     TFVQDRPGHDVRYAVDAAKIRRDLGWLPLETFESGLRKTVQWYLDNKTWWQNVLNGSY
                     RLERLGTGK"
     gene            1095..1961
                     /gene="rfbA"
                     /locus_tag="WHT44_001937"
     CDS             1095..1961
                     /gene="rfbA"
                     /locus_tag="WHT44_001937"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ2534063.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEITDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            2015..>2487
                     /gene="rfbC"
                     /locus_tag="WHT44_001938"
     CDS             2015..>2487
                     /gene="rfbC"
                     /locus_tag="WHT44_001938"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ2534064.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMRKDSPTFGKWVGEILS
                     AENKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYKPEAEQVLIWNDPTVGIGWPL"
BASE COUNT          627 a          691 c          617 g          552 t
ORIGIN      
        1 atcggttctg ccgttgtccg tcatattatc cgaaacaccc gggacgctgt cgtcaatgtc
       61 gataagctga cttatgccgg caatttggaa tctttgactg aggtagccga taatcctcgc
      121 tatgcttttg aacaagtgga tatttgcgac cgcgccgaac tcgaccgcgt attcgcgcaa
      181 taccggcctg atgccgtgat gcacttggcg gcggaaagcc atgtcgaccg ctctatcggt
      241 tcggcaggcg agtttatcca aaccaatatc gtcggcacat tcaatctgct tgaagcagcc
      301 cgcgcctact ggcaacaaat gccgtctgaa cagcacgaag ccttccgttt ccaccatatt
      361 tccaccgatg aagtctatgg cgatttaggc ggcacggacg atttgtttac cgaaaccgcg
      421 ccctacgcgc cgtccagccc ctactctgcc tctaaagcgt ccagcgacca cctcgtccgc
      481 gcgtggttgc gtacttacgg cttgccgacc attgtaacca actgctccaa caactacggt
      541 ccttaccatt ttccggaaaa actcattcct ttgatgattc tgaacgcgct tgacggcaaa
      601 ccgctgcctg tgtacggcga cggtatgcaa atccgcgact ggctgtttgt cgaagaccac
      661 gcgcgcgcac tgtatcaggt tgttaccgaa ggtgttgtcg gcgaaaccta caatatcggc
      721 ggccacaatg aaaaagccaa tattgaagtc gtcaaaacca tctgcgccct gctggaagaa
      781 ctcgctcccg aaaaaccggc cggtgtggcg cgttatgaag atttgattac tttcgtacaa
      841 gaccgccccg gccatgacgt acgctacgcc gtcgacgcag ccaaaatcag gcgggatttg
      901 ggctggctgc ctttggaaac cttcgagtcc ggcctccgca aaaccgtgca atggtatctg
      961 gacaacaaaa cctggtggca aaatgtattg aacggcagct atcgtttgga acgtttaggt
     1021 actggaaaat agttttcaga cggcatcccg acgcaatgcc gtctgaaaac ccatcgcaaa
     1081 ggaagaaaga aaagatgaaa ggcatcatac tggcaggcgg cagcggcacg cgcctctacc
     1141 ccatcacgcg cggcgtatcc aaacagctcc tgcccgtgta cgacaaaccg atgatttatt
     1201 accccttgtc ggttttgatg ctggcgggaa tccgcgatat tttggtgatt accgcgcctg
     1261 aagacaacgc ctctttcaaa cgcctgcttg gcgacggcag cgatttcggc atttccatca
     1321 gttatgccgt gcaacccagt ccggacggct tggcacaggc atttatcatc ggcgaagaat
     1381 ttatcggcaa cgacaatgtt tgcttggttt tgggcgacaa tattttttac ggtcagtcgt
     1441 ttacgcaaac attgaaacag gcggcagcgc aaacgcacgg cgcaaccgtg tttgcttatc
     1501 aggtcaaaaa ccccgaacgt ttcggcgtgg ttgaatttaa cgaaaacttc cgcgccgttt
     1561 ccatcgaaga aaaaccgcaa cggcccaaat ccgattgggc ggtaaccggc ttgtatttct
     1621 acgacaaccg cgccgtcgag ttcgccaaac agctcaaacc gtccgcacgc ggcgaattgg
     1681 aaattaccga cctcaaccgg atgtatttgg aagacggctc gctctccgtt caaatattgg
     1741 gacgcggttt cgcgtggctg gacaccggca cccacgagag cctgcacgaa gccgcttcat
     1801 tcgtccaaac cgtgcaaaat atccaaaacc tgcacatcgc ctgcctcgaa gaaatcgctt
     1861 ggcgcaacgg ttggctttcc gatgaaaaac tggaagaatt ggcgcgcccg atggcgaaaa
     1921 accaatacgg ccaatatttg ctgcgcctgt tgaaaaaata atgtttgagg ccgtctgaaa
     1981 cttttcagac ggcctttaga tgaaagataa aaagatgaac atcattgata ccgccattcc
     2041 tgacgtaaaa ctgcttgagc cccaagtctt cggcgacgcg cgcggctttt ttatggaaac
     2101 cttccgcgac gagtggttta aaacccaagt ctgcgaacgc accttcgtgc aggaaaacca
     2161 ctccaaatcc ggcaaaggcg tattgcgcgg cctgcactat caaactgaaa acacacaagg
     2221 caaactcgta cgcgtggttg tcggcgaagt attcgacgtg gccgtcgata tgcgtaaaga
     2281 ctcccccact ttcggcaaat gggtaggcga aattctgtcc gcagaaaaca aacgccaact
     2341 gtgggtaccc gaaggtttcg cacacggctt ctatgtactg agcgatgaag ccgagttcgt
     2401 ctataaatgc acggactatt acaagcctga agccgaacag gttttaatat ggaacgaccc
     2461 gacagtcggc ataggctggc cgcttca
//
LOCUS       DAWGRZ010000210         2477 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.210, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000210 DAWGRZ010000000
VERSION     DAWGRZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.210"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(148..735)
                     /locus_tag="WHT44_001939"
     CDS             complement(148..735)
                     /locus_tag="WHT44_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ2534065.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTSKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRFAALS"
     gene            899..2305
                     /gene="dnaB"
                     /locus_tag="WHT44_001940"
     CDS             899..2305
                     /gene="dnaB"
                     /locus_tag="WHT44_001940"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ2534066.1"
                     /translation="MNDYAAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGRLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          634 a          632 c          650 g          561 t
ORIGIN      
        1 cgaaacctga cagtaaccgt agcaactgaa ccgtcattcc cacgaaagtg ggaatctaga
       61 aataaaaagc aacaggcatt tatcggaaat aactgaaatt caataccgca aaaatctacc
      121 cgaaatgata tagcggatta acaaaaatca ggacaaggcg gcaaaacgtt tggcgacttc
      181 gtcccagttg acgatttccc aaaaaccttt caggtagttg ggacggctgt tgcggtagtc
      241 gatgtaatag gcgtgttccc acacgtcgca ggtcagcagc ggcgtgtttt cagtggtcag
      301 cggcgtagcg gcgttggaag tagaaaccaa atccaatccg ccggcagggg tttttaccag
      361 ccacgcccaa ccggagccga aagtaccggc cgcgcaggca ttgaacgctt cttggaattt
      421 ctcgaagctg ccccatttcg cgtcgatggc ggcggccagt tcgccggcag gtttgccttg
      481 acctttggac gtgaaaccca gccagtagaa ggtgtggttc caagtttgtg ccgcgttgtt
      541 gaacacgccg cctgaagatt ttttcacaat ctcttccaaa ggcaggtttt caaattcggt
      601 gcctttgatt tgattgttca ggttggtgat gtaggtttga tggtgtttgc cgtagtggaa
      661 ctccaaagtc tctttgctca gatgcgggga caatgcgtcc agttcataag gcagttgcgg
      721 cagcttatgt tccattttgt actcctgaat attgttttaa atgttgtatt ttggcagtgt
      781 tgctgcaaat aactcggcag cccgtgtatt ctacctgttt tgcggtgcgg aaaccaatta
      841 aacctgcttt acgctataat agaagattgc aatttcggca cgacagatag gatgtaccat
      901 gaacgattac gcagccatgc cgtctgaaga ccgtgaggtc ggcgcattgt ctctgccccc
      961 acactcaatg gaggcggaac aatccgtttt gggtgggttg atgctggaaa atccggcatg
     1021 ggacaggatt gccgatgtgg tttccggtga agacttctac cggcatgaac accgcctgat
     1081 tttccgatcc attgccaaat tgattaatga gagccgtccc gccgatgtga ttacggttca
     1141 ggaagatttg cagcggaacg aagaattgga agcggcaggc ggattcgaat atctgattac
     1201 gctggcgcaa aacaccccgt ctgccgccaa catccgccgc tacgccgaaa tcgtgcgcga
     1261 gcgttccatt atgcgccaac tcgccgaagt ggggacggaa atcgcccgca gcgcatacaa
     1321 tccgcaaggc agggacgcgg ggcagctttt ggacgaggcg gaaaacaaag tattccaaat
     1381 cgccgaaagc accgccaaat ccaagcaggg ctttttggag atgcccgatt tgctgaaaga
     1441 agtcgtacag cgcatcgata tgctctactc gcgcgacaat cccgatgaag ttaccggcgt
     1501 gccgacgggg ttcatcgacc tcgacaaaaa aacctcgggt ctgcaacccg gcgacctgat
     1561 tatcgttgcc ggtcgtccgt ctatgggtaa gaccgccttt tctatcaata tcgccgaaca
     1621 cgttgccgta gaaggcaggc tgcccgttgc tgttttctcg atggaaatgg gcggggcgca
     1681 actggtcatg cgtatgctcg gctcggtcgg acggttggat caaagcgttt tgaaaaccgg
     1741 caggctcgaa gacgaacact ggggtcgcct gaacgaagca gtcgtcaaac tctccgacgc
     1801 gcccgtgtac atcgacgaga ccccgggtct gaccgcgctc gaactgcgcg cccgtgcccg
     1861 ccgtctcgcc cgtcaattta acaataagct tggattgatt gtcatcgact acctgcaact
     1921 gatggcagga tccggccgtt ccgacaaccg agcttcggag ctgggagaga tttcacgttc
     1981 gctcaaagcg ttggcgaaag aattgcaagt ccccatcatc gccctgtcgc aattgagccg
     2041 cacggtcgaa tcgcgtaccg acaaacgccc catgatgtcc gaccttcgcg agtccggcgc
     2101 aatcgagcag gatgccgacc tgattatgtt catgtaccgc gacgaatact acaaccagga
     2161 ctcacccatg aaaggccttg ccgaatgtat catcggcaaa caccgcaacg gtcccgtcgg
     2221 taaaatcttc ctcacatgga cgggacaatt caccaaattc gacaatgctg cctatattcc
     2281 cgaggaggca aagatagagg attaaatggc tatataaaaa tttattaggc gaaatcaggc
     2341 aaaatcgttt aaaatcatgc tgagagattg ccctaaaaaa taaaacgcgg tcttgaggca
     2401 tttttgcatt cagcccgcat ataattgaaa atatagtgga ttaacaaaaa tcaggacaag
     2461 gcaacgaagc cgcagac
//
LOCUS       DAWGRZ010000211         2475 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.211, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000211 DAWGRZ010000000
VERSION     DAWGRZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.211"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            35..1390
                     /locus_tag="WHT44_001941"
     CDS             35..1390
                     /locus_tag="WHT44_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ2534067.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWIELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2404
                     /locus_tag="WHT44_001942"
     CDS             1394..2404
                     /locus_tag="WHT44_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ2534068.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     SYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHMPADAEKLPVSTVSDDLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATIAYGIES
                     LS"
BASE COUNT          456 a          664 c          747 g          608 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcaaatg agccgcattt ttcaaacgct
      181 aggcgcagac ccgcacaatt tgggctggtt tttcatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt tgggcggccg
      301 ccgtctgccg tatctgcttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttagacg tgtcgtcaaa tatggcgatg cagccgttta agatgatggt
      481 cggcgacatg gtcaacgagg agcagaaagg ctacgcctac gggattcaaa gtttcttagc
      541 aaatacgggc gcggtcgtgg cggcgattct gccgtttgtg tttgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccgca gaccgtggtc gtggcgtttt atgtgggtgc
      661 ggcgttgctg gtgattacca gcgcgttcac gattttcaaa gtgaaggaat acgatccgga
      721 aacctacgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggat
      781 cgaactcttg aaaaccgcgc ctaaggcgtt ttggacggtt actttggtgc aattcttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccacc gatgcgtctt ccgtaggtta tcaggaggcg ggtaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtgacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggct tgtttaacgg ctctatctgt atgcctcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttccgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccgcgat ttcggtctga tcgacgggcg
     1441 tgccgtaacc ggctatgtgc tgtccaaccg gcgtggtacg cgtgtctgcg tgctggactt
     1501 gggcgggatt gtgcaggaat tttccgtttt ggcagacggc gtgcgcgaaa acctcgtggt
     1561 gtcgttcgat gatgcggctt cctatgcgga caatccgttt cagattaaca aacagatagg
     1621 gcgcgtggcc ggacgcatcc gcggtgcggc gttcgacatc aacggcagga cttaccgcgt
     1681 ggaggccaac gaaggcagga acgcgctgca cggcggttcg cacgggctgg ccgttacccg
     1741 tttcaacgcg gtggcggcag acggccgttc ggtggtgctg cgcagccgcc tgcaacagtc
     1801 ggccgacggt tatcccaacg atttggattt ggatatttcc taccgcttgg acgaggacga
     1861 ccggcttacc gttagctatc gcgccaccgc gctcggcgac acggtgttcg acccgacgct
     1921 gcacatttac tggcggctgg acgcgggcct gcacgatgcg gttctgcata ttccgcaggg
     1981 cggacatatg ccggccgatg ccgaaaaact gcccgtctca acggtttcag acgacctcga
     2041 agtatttgat ttcagccggc ccaagccgct ggatgccgcc gttgccgccc tgcgccgcga
     2101 aacgggtcgg gccggttttg acgacgctta ccgcgtgccg tccgatatag gccgtcccgc
     2161 cgctgtgttg caagccggac gccgccgtcg tatcagcata tacagcgacc gcaatggctt
     2221 ggtcatcttt accgccgccc cgcaggattt cgcgcggcac gatgcgggcg tttacgacgc
     2281 gctggcgacc gaggcgcaga cgctgcccga cagcctgaat tggcccgagt tcggcaatat
     2341 tcgtctgaac aagggtgata ccagggaggc gacgattgct tacggcatcg aatccctttc
     2401 ttaggagctt cctaacaccg gttgcagacg acctttttat agtggattaa caaaaaccgg
     2461 tacggcgttg cctcg
//
LOCUS       DAWGRZ010000212         2468 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.212, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000212 DAWGRZ010000000
VERSION     DAWGRZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.212"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(13..819)
                     /locus_tag="WHT44_001943"
     CDS             complement(13..819)
                     /locus_tag="WHT44_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ2534069.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGCGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRSF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1039..2409
                     /gene="ffh"
                     /locus_tag="WHT44_001944"
     CDS             1039..2409
                     /gene="ffh"
                     /locus_tag="WHT44_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ2534070.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFINNVKEKALGQEVAGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLAGRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLHKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMRGIK
                     GMFPGL"
BASE COUNT          683 a          619 c          642 g          524 t
ORIGIN      
        1 tgttggctct tcttatcttt tcagaatgat ttccaatacg aacttgctgc ccatataggc
       61 aatcataagg ctgacaaatc cgatgatggt ccacacggcg gcttttttgc cgcgccatgc
      121 ggtcatgctg tgcttgagca gcagtccgcc gtaaatcagc catgacaata tgccgaatac
      181 ggttttatgg gtaaaggtca tgggtttgcc gaatacggct tcggcaaaaa atgttccact
      241 gacgacggaa taggtcagca ggatgaaacc tgcccacatg gcctggaaca tgagtttttc
      301 caaactgagc agcgacggca ggaatcctgc gagcttggag aagctcctgc ggtgcaggct
      361 ccgattcagc agcagggtca aaacggacaa taatgttgcg atgccgaaca gcccgtatgc
      421 gagcagcgaa gttccgatat gcagcataaa gggaaggtcg gtaatttcat atcccgagaa
      481 ttttccagga aaaaccaaac ctgacagcag catcagtgcg gcgcaaggat acagcagcaa
      541 ctgcactccg cgcagcggat aaaagaagct gccggcaaaa taaataaaca gcatcatcca
      601 aacaatcagg ctgccggaat acccgaagcc cataatgatg attttgtctt gaatgaccgg
      661 cataagcagt gccgcgccgt ggacggtcaa tgccgcaccc aaaaccggca attccgtctt
      721 ccacgggtaa tcccggccgc acccctgctg ttggcagtgc catgcaaatg cacccaatcc
      781 tgcgtaaacc gccgtcaaaa agatgaaaac tgtcggcatg gtggactttc tctatctata
      841 ctgttgcgcc gtatgcggcc gcttatgaaa tattggaact tttaacgttg gaattgtaaa
      901 atccccattt cggtcaagcc ttgacggatt tgccgatatg ctgtccggca cacaagccgc
      961 atcaaattat tttgatttta ttttaacaaa gaatgcccct gatggggcaa gctattctta
     1021 ttcagaccaa ggaccagtat gttagacaat ttaaccggcc gcttcagcaa tgtcttcaaa
     1081 aacatccggg ggcaggccaa actgaccgaa gacaatatta aagaggcctt gcgcgaagtc
     1141 cgcctcgccc tgcttgaggc ggatgtcgcc ctgcctgtcg tcaaagagtt catcaacaac
     1201 gtcaaagaaa aggccctcgg tcaggaagta gcgggcagcc tgacgccgga tcaggcattt
     1261 atcggcgtgg tcaacaaagc cctgaccgaa ctgatgggca gggaaaacaa aacgctggat
     1321 ttgtcggttg cgccgcccgc cgtcgtgttg atggcaggtt tgcagggcgc aggcaagacg
     1381 acgaccgtcg gcaaactcgc ccgcctgttg aaaaacgatc agaagaaaaa ggttttggtg
     1441 gtatccgccg acgtttaccg tcctgccgcg attgaacagc tgcgtctgtt ggccgaacag
     1501 gtcggcgtgg attttttccc gtccgatacc aaccaaaaac cggttgaaat tgcaactgcc
     1561 gccgtcgatt acgccaaaaa acatttttac gatgtattga tggtcgatac cgccggccgt
     1621 ttggcaatcg atgaagagat gatgaacgaa atcaaagccc ttcacgcggc ggttaacccg
     1681 gtggaaactt tgttcgtcat cgatgcgatg ctgggtcagg atgcggtgaa cactgctcag
     1741 gcatttaatg aagccctgcc gctgaccgga gtcgtattga ccaagatgga cggcgactcg
     1801 cgcggcggtg cggcattgtc cgtacgccac gtaaccggca aaccgattaa atttatcggt
     1861 gtcggcgaaa aaatcaacgg cctcgaacct ttccaccccg accgtcttgc cggccgcatt
     1921 ttgggtatgg gcgacgtatt gaccctgatt gaagacgttc aaaaaggtat agacgaagaa
     1981 gccgccgcta aaatggcgaa aaagctgcac aaaggcaaag gcttcgacct caacgacttt
     2041 aaagaacaaa tccagcaaat gcgcaatatg ggcggtttgg aaaacctgat gtcgaaaatg
     2101 ccgggcgaac tgggtcaaat ctcgaaacaa atccccgaag gaacggctga aaaagcgatg
     2161 ggcaaagtag aagccatcat caactcgatg acccctaaag aacgcgccaa ccctgccctg
     2221 ctcaaagcca gccgcaaacg ccgtattgca atgggtgcgg gcacaaccgt gcaggaagtg
     2281 aacaaattgc tcaaacagtt tgaacaaatg caacaaatga tgaagatgtt cagcggcaac
     2341 ggcttgggca aactgatgcg tatggcgaaa ggaatgaggg ggataaaagg gatgttcccg
     2401 ggtttgtaag ccgatttaac agaaaacgcc gtctgaaatt tcagacggcg tttttgtttt
     2461 atattctg
//
LOCUS       DAWGRZ010000213         2457 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.213, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000213 DAWGRZ010000000
VERSION     DAWGRZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.213"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..372
                     /locus_tag="WHT44_001945"
     CDS             <1..372
                     /locus_tag="WHT44_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEZ2534071.1"
                     /translation="RLRRLVLIFVNPLYIIATTVPAQQAFLDAAELMQWSIETEGLGL
                     NVISHKILGKDHAQVEFEAYFRDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQP
                     CICGSGKKFKACCGKYLKPVA"
     gene            complement(590..1384)
                     /locus_tag="WHT44_001946"
     CDS             complement(590..1384)
                     /locus_tag="WHT44_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ2534072.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDSPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            1488..2213
                     /locus_tag="WHT44_001947"
     CDS             1488..2213
                     /locus_tag="WHT44_001947"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ2534073.1"
                     /translation="MPRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRQPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          651 a          782 c          544 g          480 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat acatcatcgc tactaccgtt
       61 ccggcgcaac aggcattcct cgatgccgcc gaactgatgc aatggagtat agaaaccgaa
      121 gggctgggct tgaacgtcat ctcgcacaag atactcggca aagaccacgc ccaagtcgaa
      181 tttgaagcct acttccgaga cggacaacac cgatccgcgc atcacgaact gtccggcttc
      241 gtcaacatcg gcggacaatg gtattttatc gatcccaccg ttccgcatcc tgcgatgaaa
      301 caaccctgca tttgcggatc aggcaaaaaa ttcaaagcct gctgcggcaa atatctgaaa
      361 cctgtcgcat aaaaatgccg tctgaacgtt cagacggcat tttcaacgtg caaaaaaaac
      421 cattcatacc aagggtaagt atgaatggtc aatacattgc gggaaaacgt cttacttgct
      481 gcactgccga aaagggagaa acggcagcgg taatcagcgg aaaggattgt acccgaatta
      541 atattaagaa acgttaatcg cgaaaatata ttaacaaacc tgttgaaacc tattggtttt
      601 cccgtatcca cccgacccag cgttcaaaca gcttcggttc gagcgcggca acgaccgagc
      661 gtttgaacac gtgttcacca ctccaaaacc cgtcgccttc caaagtcgtc agcctgccgc
      721 ccgcctcctc gaaaatcaac gcgccggcgg cataatccca cagcttctgc ccgccgtgaa
      781 cataaacatc ataacgcccg cacgccagat aacaccaatc caacgtactg ctgcccatac
      841 tccgtatcgt tccaaaaggc gcgagcgtac tcatacggct ggaaagtttg cccgaacgca
      901 gatatttgat ttccacgccc gcaatcgcct cattgagttt tttatccacg aggcgcaggg
      961 gcagacgcgt cccgtttaaa aacgccccct gcccgcgttc ggcataaaaa cattcgccgc
     1021 tgactgggtt gtagattacg cccaactcgg cgcgcccgtt gcggacaaac gccaccgata
     1081 ccgcaaaatg cggcagcccg ttgacaaaat tgttcgtccc gtctatcgga tcgacaatcc
     1141 acagcccctt ttcccccgaa tattgttccc acaaagccga ctgttcctgc cgcgacattt
     1201 cctcacccaa catcggactg tcgattaaaa gcggcaacgc ggcggcaaaa gccgtctgcg
     1261 cggcaatgtc cgcctcgctc aacatcgaac cgtcttcctt gcggtgagac ggcgtattca
     1321 aaaaacgcgg cataatttcg gtttgcgcga tatggcgcac gactttctgc aaacggtgta
     1381 acacttccta ctgtcctcat attttgaact tgcggcgcgc gaacgtataa tgtccgcttc
     1441 catcacgccg ctgcgacgga ttataaccgt ccgaaccgcc aaaaactatg ccccgattcc
     1501 acctgcccga aaacctttcc gtcggacaaa ccgtcgccct gcccgacaac atcgtccgcc
     1561 acctcaacgt cctgcgcgtc cgccccaacg aaaacatcac cctcttcgac ggcaaaggca
     1621 aggcacacgc cgcacggctg accgttttgg aaaaacgccg cgccgaagcc gaaatcctgc
     1681 acgaagacac aaccgacaac gagtccccgc tcaacatcac actgatacaa tccatctcct
     1741 ccggcgatcg catggatttc accctgcaaa aaagcgtcga actcggcgta accgccatac
     1801 agcccgtcat cagcgaacgc tgcatcgtcc gcctcgatgg ggaacgcgcc gccaaacgcc
     1861 tcgcacgctg gcaggaaatc gtcatctccg cgtgcgaaca aagcggcagg aacaccgttc
     1921 cccccgtact gcccatcatc ggctaccgtg aagcactcga caaaatgccg tctgaaagca
     1981 ccaagctgat tatgagcatc aaccgcgccc gcaaactcgg cgacatacgc caaccgtccg
     2041 gcgcaatcgt ctttatggtc gggcccgaag gcggctggac agaacaggaa gaacaacagg
     2101 catttgaagc tggctttcag gcggttacac tcggcaaacg gattttacgc acagaaaccg
     2161 ccccactcgc cgccctcgcc gccatgcaga cgctttgggg cgatttcgca taaacagaaa
     2221 tgccgtctga aacccgttca gacggcattt tgcagccgat taagatagta ggttcaaata
     2281 agatttcccg tgtcgtcatt cccgcgaaag cgggaatcta gaaacgaaaa actacagaga
     2341 tttatccgaa acaacaaccc tctccgccgt cattcccgcg caggcgggaa tctagaaacg
     2401 aaaaactaca gggatttatc cgaaacaaca aaccctctcc gccgtcattc ccgcgca
//
LOCUS       DAWGRZ010000214         2454 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.214, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000214 DAWGRZ010000000
VERSION     DAWGRZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.214"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(119..1570)
                     /locus_tag="WHT44_001948"
     CDS             complement(119..1570)
                     /locus_tag="WHT44_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ2534074.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            complement(1593..2213)
                     /locus_tag="WHT44_001949"
     CDS             complement(1593..2213)
                     /locus_tag="WHT44_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ2534075.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
BASE COUNT          767 a          581 c          574 g          532 t
ORIGIN      
        1 ggaataagaa ttcgggacaa aatgtttaaa ccattttgtc ccgactgctg tgatgcggtt
       61 tttttgaact agcaggacgt tacctaaaac ctgcgttgcc tgaaaccctt taagacggtt
      121 aaatccctct gccgtatttg tattctacaa tcatattatc gtcggtaata acttccgccc
      181 cagccgaagg ttccgtcatc tgaatcagca tacgagagac aaccttttgt gctgcagcat
      241 ccacggtgct gctgtcaaat acgtgcctgc cgctttccgg ccaaatcaac cgggagagac
      301 gttgcttgag cagttcttta ttagggaaaa ctaccggggt tgccgagccg actaccatat
      361 gcccatagcg gtatgcatag ggaatactgt gtacggcggt agcaaaagca tgcgggctgt
      421 gcgtggtatt aaacattaca ataccatccg gggtaaggtg gctttgcacc tgttttaaaa
      481 attccgcact caacaggttg gtggaatagg cacgccagta ccaagtcgta ttcatcaaaa
      541 tcaggtcgaa tttttcatca ggatggcgac gcagccattt cctaccgtca tccaatacaa
      601 tttcaacacg tttgtcctgc aaaagcgggg cgatttgcgg ctcgtccgcg ataaggctac
      661 ggtatgccgg attgatttcc gcaacgatca tcgactgcat ttccggaatg gcagacaaga
      721 cgcgcgccca cgaacctgta ctcagtccaa cgacgaaaat gcggcgtatg ccagacttca
      781 gggagggtag cagataggca cgttcgatgc cgttgacact attgaataca tcggtattgt
      841 atgcgccgtc gtatacattc gccccataaa caaccttatc accatctcta tggtaaaccg
      901 caacaatgcc gtgtttgttt tcaatcagcc tatccggacg gtcagcaata ttttgaaaga
      961 cagaatccgg cagtaggaac atgaggatgc cgaacattag ggaaactgct accgacactg
     1021 cattcagtcg gagacttttt tggaacagtg tacaaaacaa agggacagca gcagaaatca
     1081 aacagatgag caggtaaatc tgttgggtgg acaagaaatc aagtatcaca aagccgataa
     1141 ggaccggacc caatgcactg ccggcaacgt tggcgaaata aacattggaa acctgtcgtc
     1201 cggatttgtt gccatccgta cccacatggt gtacgagcgg gaaaatcaac cctctgacga
     1261 cggcagacag ggtaatgaag ataccggcgt ggtggacgaa gccggaaaaa cccgtcaaca
     1321 accacgcagc acccaaaatc aaaaagtcgg caatacccgc ccacaagaag cactgcccga
     1381 taaagggaat atcaacaaag cggctgcggc aaatccgttt gccaaaatac gcgccgacgg
     1441 cgataccggt cagaaaacag gcaagggtaa atgaaaatgc ctgaggcacg gactgtgctg
     1501 cgaacgaaaa catcctcacc cacaagactt ctatacccaa gctcaataag ccgctaagga
     1561 aagaaagcat ataaatcaaa ctagtattcg gtttagtgtt cactatatcc atcctttctg
     1621 taacgcagcc atactgaagc agcaatcaga aggttaaagc aggctgtcag cgcaatggtt
     1681 tgggagaggg taaaaaagac gtagaaaaat tcggcggcgg caagcgatcc gagtgccgca
     1741 cccaaagtgt tgaaaaaata taaggtaccg atagactcgc caacattatg tattttccgg
     1801 ttaaaaaaac aggtcagcaa gggcaaggtc gcgcccatca taaaggtagg aagcagcaat
     1861 aagaggaaat tggcagcagc gatgatgggc aaatcagcct caactaaaag atgccccaag
     1921 ccggaaatca gacccctgct taccaaaccg aacagaccga tggatacttc agcgatgcaa
     1981 aacaggggga tgatacttga aggaaaacgg tcagcaatgc gtccaccgaa atacgcacct
     2041 acacccaagc cgaccataaa tacagaaata atgacagtaa tcgaactcaa atcgatacct
     2101 atgtgactga atagaagcct ctgccagctg acctggtaaa tcagggcgca gaatccagag
     2161 gcgaaaaaca ccagcgacaa tcctttcagg gttttacttg cggtcgaatt catattttct
     2221 attttcaaat aggcggaact ataaatgaaa tgtatcaatg tgtaaacatt tgtattaacc
     2281 gtaggaaatc accgtattgc tcaaactgca ggccgggaaa ataaactcgt aagcccgcaa
     2341 acggcgtatc actgttcaaa ctgctgtgcg gcttcacggt gttataaaaa taatggggct
     2401 gtcctggaca actaagataa actcgatttt actaattgtt ttaaaatgga aatt
//
LOCUS       DAWGRZ010000215         2339 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.215, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000215 DAWGRZ010000000
VERSION     DAWGRZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.215"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(84..1493)
                     /gene="dacB"
                     /locus_tag="WHT44_001950"
     CDS             complement(84..1493)
                     /gene="dacB"
                     /locus_tag="WHT44_001950"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ2534076.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLDAQKQLREQGILNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAAGSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGIADTPEG
                     AQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1658..2224
                     /locus_tag="WHT44_001951"
     CDS             1658..2224
                     /locus_tag="WHT44_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ2534077.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          539 a          563 c          666 g          571 t
ORIGIN      
        1 cattttaaaa caattagtaa aatcgagttt gtcctagtta tctaggacag ccccttaaat
       61 ttaatccact atattttcct gtttcaggct cggcgttctt tgcacatcag tttcgcatcc
      121 agccagccat cgccgccgga gatgatgttg ttggcaacga agttgtccaa gtctggcagc
      181 agggaaacgg cgcggccgct gttgatgatg acgaccaccg ccatcggttt gtcgcccagc
      241 caataacctg caagggcgcg gacattgttg agcgtgccgg tttttaagcg caacagcccg
      301 ccgctttgtt tgaagcggtt gcgtaaagtt ccgtctgtgc cggcgatggg tagcgtgtcg
      361 atgaaatctt gtgcaaacgg gctgaaataa gccgtttcca acatttgcgc catcattctc
      421 gccgttaccc tttcttttct ggacaggccc gaaccgtttt ccaaaaccaa atccgcaaca
      481 tcgatgcccg atacggcaag ttcgcgccgg acggcagacg ccgcctgttc ggaaacggcg
      541 ggcagtttgc cgtcgccgcc gagtttgagg aagacggaac gcgcaattag attgtccgaa
      601 cgcttgttca tgtccgtcaa aatttctttc atcggtttgg cgtgtgcaac ggcaagtgtc
      661 tgcgcgcctt ccggcgtgtc ggctatgccg ataccgtctg aaatccgtcc gccgccgagc
      721 agccagtggt tggtaaaact ttgccggatc agttcgtcaa gcgcgaacat ccggacaccg
      781 acaggcttgc ccaaacagct ctcgggaata ttgccgcgca atttcagcgt attgtccgaa
      841 aaagatgcac gcatcagttt tttgatcgaa gggcaggcag cttgggaggc ggtaattttc
      901 aagttgtttt gggcgaaaat atgcggcaaa ggcggatcgg tgaggatgtc ggtactgccg
      961 gcggcattgc gttcggcgcg caccataacc ataccggcag acagcatagt tggattgggg
     1021 ggcgtcataa acggcgaacc gctgtcggct tcgaaatcgt cggggctgcc gacttcgccc
     1081 cacaggctgt ggtcgagcat caggtgtccc gtgatattga gtatgccttg ttcgcgcaac
     1141 tgtttttgag catcaagcag gttttcctga ttgaaaacgg ggtcgccgct gcccgcccaa
     1201 tataggtttc cgtcaagcgt gccgtcgttt accgtaccgt tgcttttaaa ctcggtcgcc
     1261 cagcggtaat tgctgccgaa ggttttgaag gcggcaaacg cggtaacgag tttcattgtg
     1321 gaggcggggt tgacggggac atccgagcgg tggtcaatga tgacttttcc gctgtcaagc
     1381 tcttggacat atacggcgat ttcgttttgc ggaatgcggc cggtatcgag cgcgtgtgcg
     1441 gcgagggagg cgagaagcag cagcagggag gccgctgttt tggggaaatt cataggtaaa
     1501 tctgttacat aaaaaaagca attataaggc aaagccggat aagtgggaac gctgtggcgg
     1561 cgggccggct tgtttgcagg ggaaatcaca tatataataa tcgctatcat tatgaaatga
     1621 ttgagacgca caaacttaat attcaggagg aatgattatg gctaagaaaa tcagtatttt
     1681 ggtgggcagc ctgcgccgtg cttcttttgc gcgcaaagtg gcattgaatg cggcggagat
     1741 gttccccgaa ggctggcagg cggaaatcgt tgaaatcgga catctgccgc tttacaattt
     1801 cgattatgac gaccctgcgg tggaagatgt gccgctaccc gaaagctaca cggctttccg
     1861 cgaaacgatt aaggcttcgg acggcatttt gttcgttacg tccgaaaata accgcaccat
     1921 tcccgcctgt ttgaaaaatg cggtggacat cggctcgaaa ccgaatgccg acgtggcgtg
     1981 gaaaaacaaa ccggcaggca tcatcagcca ttccgtcggc aagatgggcg gttacagttc
     2041 ccaaaaaaac ctgcgccttg cgctgtctta tttcgatatg cccgtaaccg gacagccgga
     2101 agtgtttttg ggcaattcgc cgacgctgtt tgatgaaaac ggcaaattga ttgactcggc
     2161 aagggatttt gttcagtcat atattaatca gtttgtcggt ttgattgaaa gaaatgccaa
     2221 ataaacaaac caagtcgaaa ggccgcaaaa ccggttcggg ttttgcggct tttttatatg
     2281 ggctgggcag cgtcaaggtt tgccgtttgc cggataatgc gcggcaaatg accggcgtg
//
LOCUS       DAWGRZ010000216         2320 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.216, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000216 DAWGRZ010000000
VERSION     DAWGRZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.216"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..2132
                     /locus_tag="WHT44_001952"
     CDS             <1..2132
                     /locus_tag="WHT44_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ2534078.1"
                     /translation="NANNPNKSTKAPSPALTAPALWFGPVQNGKVQMYSASVSTYPDS
                     SSSRIFLQNLKRKNDPNKPGRYSLADLSENEIKSKEPSFTSRQTVIRLDKGVHQIKLQ
                     GNEVANFNGNDGKNDTFGIVSEGSFMPDASEWKKVLLPWTVRASNDDGQFNTFNKEEK
                     DGKPKYSQKYRSRDNGKHERNLGDIVNSPIVAVGEYLATSANDGMVHIFKKGNGGDER
                     NYSLKLSYIPGTMPRKDIQNTESTLAKELRAFAEKSYVGDRYGVDGGFVLRKVERNGK
                     DHVFMFGAMGFGGRGAYALDLSKIDSGNGNLADVSLFDVKHDKNGNNGVKLGYTVGTP
                     QIGKTHDGKYAAFLASGYATKDITSGDNKTALYVYDLESSGTLIKKIEVPGGKGGLSS
                     PTLVDKDLDGTVDIAYAGDRGGSMYRFDLSNQDPNQWSVRAIFEGTKPITSAPAISQL
                     KDKRVVIFGTGSDLSEDDVLSTSEQYIYGIFDDDTVANNVNVKLSGLGGGLLEQELKQ
                     EDKTLFLTDYKRSDGSGSKGWVVKLKGGQRVTVKPTVVLRTAFVTIHKYTGTDKCGAE
                     TAILGINTADGGKLTKKSARPIVPAENQAVAQYSGHKKGINGKSIPIGCMQKGNEIVC
                     PNGYVYDKPVNVRYLDEKKTDGFSTTADGDAGGSGIDPAGKRSGKNNRCFSQKGVRTL
                     LMNDLDSLDITGPTCGMKRISWREVFY"
BASE COUNT          649 a          588 c          612 g          471 t
ORIGIN      
        1 tcaacgcaaa taaccccaat aagagtacca aagccccttc ccccgcactg actgcccccg
       61 cgctgtggtt cggacctgtg caaaatggca aggtgcagat gtattccgct tcggtttcca
      121 cctaccccga cagttcgagc agccgcattt tccttcaaaa tctgaaaaga aaaaacgacc
      181 ccaacaaacc cggccgctat tccctcgcag acttgagtga gaatgagatt aaaagtaaag
      241 agccgagttt cacaagtcgg caaaccgtca tccgattgga taaaggcgta catcagatca
      301 aacttcaagg caatgaggtc gccaatttta atggaaatga cggcaaaaac gacactttcg
      361 gcattgttag tgaagggagc ttcatgcctg atgccagcga gtggaaaaaa gtattgctgc
      421 cttggacggt tcgtgcttcc aatgatgacg gtcaatttaa cacattcaac aaagaagaaa
      481 aagacggcaa gccaaaatac agccaaaaat accgcagccg cgacaacggc aagcacgagc
      541 gcaatttggg cgacatcgtc aacagcccca tcgtggcggt cggcgaatat ctagctactt
      601 ccgccaacga cgggatggtg catatcttca aaaaaggcaa cggcggggac gagcgcaact
      661 atagtctgaa gctcagttat atcccgggca cgatgccgcg caaggatatt caaaacaccg
      721 aatccaccct tgccaaagag ctgcgcgcct ttgccgaaaa aagctatgtg ggcgaccgct
      781 acggcgtgga cggcggcttt gtcttgcgca aagtcgaacg gaacgggaaa gaccatgtgt
      841 ttatgttcgg cgcgatgggc tttggcggca gaggcgcgta tgccttggat ttaagcaaaa
      901 tcgacagcgg caacggcaac ctggcagacg tttccctgtt tgatgtcaaa catgacaaga
      961 atggcaataa cggcgtgaaa ttaggctaca ccgtcggcac gccgcaaatc ggcaaaaccc
     1021 acgacggcaa atacgccgct ttcctcgcct ccggttatgc gactaaagac attaccagcg
     1081 gcgacaataa aaccgcgctg tatgtgtatg atttggaaag cagcggcacg ctgattaaaa
     1141 aaatcgaagt acccggtggc aagggcgggc tttcgtcccc cacgctggtg gataaagatt
     1201 tggacggcac ggtcgatatc gcctatgccg gcgatcgcgg cggcagtatg taccgctttg
     1261 atttgagcaa tcaagatcct aatcaatggt ctgtacgcgc catttttgaa ggcacaaaac
     1321 cgattacttc cgcgcccgct atttcccaac tgaaagacaa acgcgtggtt atcttcggca
     1381 cgggcagtga tttgagtgag gatgatgtac tcagtacgag cgaacaatat atttacggta
     1441 tcttcgacga cgatacggtg gcgaataacg taaatgtaaa actcagcggt ttgggaggcg
     1501 ggctgctcga gcaagagctt aagcaggagg ataaaacctt attcctgacc gattacaagc
     1561 gatccgacgg atcgggcagc aaagggtggg tagtgaaatt gaagggcgga cagcgcgtta
     1621 ccgtcaaacc gaccgtggta ttgcgtaccg cctttgtaac catccataaa tatacgggta
     1681 cggacaaatg cggcgcggaa accgccattt tgggtatcaa taccgccgac ggcggcaagc
     1741 tgaccaagaa aagcgcgcgc ccgattgtgc cggccgagaa tcaggctgtc gcgcaatatt
     1801 ccggccataa gaaaggcatc aacggcaaat ccatccctat aggttgtatg caaaaaggca
     1861 atgaaatcgt ctgcccgaac ggatatgttt acgacaaacc ggttaatgtg cgttatctgg
     1921 atgaaaagaa aacagacgga ttttcaacaa cggcagacgg cgatgcgggc ggcagcggta
     1981 tagaccccgc cggcaagcgt tccggcaaaa acaaccgctg cttctcccaa aaaggggtgc
     2041 gcaccctgct gatgaacgat ttggacagct tggacattac cggcccgacg tgcggtatga
     2101 aacgaatcag ctggcgtgaa gtcttctact gatttgcacg cgaaaatgcc gtccgaaagg
     2161 ttttcggacg gcattttttg cgtttttcgg gaggggcggg ttcgtaaaag gcgggctata
     2221 gggtaggctt catctcgcca atctcactga atccatcaat ttccacaatt caattaaata
     2281 ccgtcaaacc gatgccgtca ttcccgcgca ggcgggaatc
//
LOCUS       DAWGRZ010000217         2285 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.217, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000217 DAWGRZ010000000
VERSION     DAWGRZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.217"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            19..747
                     /gene="rpe"
                     /locus_tag="WHT44_001953"
     CDS             19..747
                     /gene="rpe"
                     /locus_tag="WHT44_001953"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ2534079.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRRV
                     RAMLDRYEAQSGRHIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            complement(770..1801)
                     /gene="ruvB"
                     /locus_tag="WHT44_001954"
     CDS             complement(770..1801)
                     /gene="ruvB"
                     /locus_tag="WHT44_001954"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ2534080.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDRFGIVSRLEFYESRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(1883..2191)
                     /locus_tag="WHT44_001955"
     CDS             complement(1883..2191)
                     /locus_tag="WHT44_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534081.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQIRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
BASE COUNT          455 a          546 c          738 g          546 t
ORIGIN      
        1 gatattgaag gaaaaaacat gactacttac cgtatcgcac ccagcatttt atcggcagac
       61 tttgcccgcc tcggagaaga ggtggaaagc gtcattgcgg caggtgcaga tctgatccat
      121 tttgacgtga tggacaacca ttatgtgccg aacctgacct tcggccctat ggtttgcgcg
      181 gcgttgaagc cttatgcaag cgtgccgatt gatgtgcatc tgatggtcga acccgttgac
      241 gacctgattc agtcgtttgc caaagcagga gcatcaatca tcacgttcca tcccgaggcg
      301 agccgccata tcgaccgcag cttgagcctg attcgtgata tgggctgtca ggcggggctg
      361 gtgttgaatc cggcaacgcc cgtatatctg ttggaaaacg tattggacag gctggatatg
      421 gttttgctga tgtcggtcaa ccccggattc ggcggacaaa gcttcatccc gcacaccctt
      481 gaaaaaatcc gccgggtgcg ggcgatgctg gatcggtacg aagcacaaag cgggcggcac
      541 atcgccatcg aagtggacgg cggcatcaaa accgacaata ttgccgccgt tgcccgagct
      601 ggtgcggata cctttgttgc cggttctgcg attttcggca aaccagacta taaggcggtg
      661 attgatgcga tgagggcgga actggaaaaa agcggcaggc tgaaccttgc gccaatgatt
      721 gaaatagaaa tgccgtctga aaattagctc tgtttcagac ggcattgcgt tatttttcga
      781 cgggcagccc gaaatgcagg taggcgcgtt cggtcgccat cctgccgcgc ggggtgcgtt
      841 gcaggaagcc ttgttggata aggtagggtt cgataacgtc ttcgatggtg tctgtagatt
      901 cgccgatggc ggcggcaaca ttgtccaaac cgaccgggcc gccgccgaat ttgtgcaaaa
      961 cggcttcgag aaatttcctg tccatcacgt ccagcccctg cgcgtccacg tccagcatac
     1021 ttaaagcggc atcggcgatg ccgccgtcga ttgtgccgtt gtttttcacg tcggcgaaat
     1081 cgcgcacgcg tcgcaacagg cggttggcga tgcgcggcgt accgcggctg cgtttggcga
     1141 tttcttccgc gccttcttcg gacatatcga gctgcaacag ttgtgccgaa cggctgacga
     1201 tggtggtaag gtctcggctt tcgtaaaact caaggcggga gacgatgccg aagcggtcgc
     1261 gcaacggatt ggtcagcata ccggcgcggg tggtcgcgcc gatgagcgtg aagggcggca
     1321 ggtcgatttt gacggaacgg gcggcgggtc cttcgcctat cataatgtcg agccggtagt
     1381 cttcgagcgc gggatagagg atttcttcga caacagggct gaggcggtgg atttcgtcga
     1441 tgaacaatac atcgtgcgga tcaaggttgg tcaaaagggc ggcgaggtcg cctgcgcgtt
     1501 cgaggacggg gccgctggtt tggcgcaaat ttacgcccaa ttctttggcg atgatgtgcg
     1561 ccagtgtggt tttgcccagt ccgggcgggc cgaagagcaa aacgtggtcg agtgcttcgc
     1621 cgcgtttttt ggcggcttgg atgaaaatgg caagctgttc tttggctttg tcttgcccga
     1681 tgtagtcgtc cagcgttttg gggcggaggg cgcgttcgag cagttcttcc tgtgcggagg
     1741 cggtttgggc ggcaacgatg cgttgcggtt gcgcggcggt caggttgtcg gtttgcagca
     1801 tagtgttccc tttgtcgggt atgccgtccg aacggtcggc ggcgtttcag acggcattga
     1861 aaagataacg gtcaaaagcg ttttatccgc gggtttcgcg ccaaacgaga tagtcgcgca
     1921 ggggcggcat aggcgggttg atgcagtggg ggcagatgcc ggtggtgtct tcgtcgtcgc
     1981 tgtcgatgtg gtaggcgttg gggtcgaagc ggatttgctg catgacggct tgcgccaatg
     2041 cctgcgcctg aacaacgtcg aaattgaatt tttcgaggat ttcgttcaac aggcggattt
     2101 cgccaccgct aaattccgca ctgtgtttga ggatgaggcg ttgataatct tcgttgttga
     2161 ttttaggcaa taaatcggtt ttcagggtca tgattttatg tcgggggcgg aatggctgaa
     2221 gcgtgaatta tagcggatat ggcggcggct gttgcaaagc ttggattggg tatcgtcctc
     2281 aaaac
//
LOCUS       DAWGRZ010000218         2218 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.218, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000218 DAWGRZ010000000
VERSION     DAWGRZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.218"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..638
                     /gene="mafB"
                     /locus_tag="WHT44_001956"
     CDS             <1..638
                     /gene="mafB"
                     /locus_tag="WHT44_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ2534082.1"
                     /translation="LGNLSPEAQLAAATALQDSAFAVKDGINSAKQWADAHPNITATA
                     QTALAVAEAATTVWGGKKVKLNPTKWDWVKNTGYKKPAARHMQTVDGEMAGGNRPPKS
                     ITSNSKADASTQPSLQAQLIGEQISSGHAYNKHVIRQQEFTDLNINSPADFARHIENI
                     VSHPTNMKELPRGRTAYWDDKTGTIVIRDKNSDDGGTAFRPTSGKKYYDDL"
     gene            648..854
                     /locus_tag="WHT44_001957"
     CDS             648..854
                     /locus_tag="WHT44_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534083.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            987..1238
                     /locus_tag="WHT44_001958"
     CDS             987..1238
                     /locus_tag="WHT44_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534084.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            1235..1606
                     /locus_tag="WHT44_001959"
     CDS             1235..1606
                     /locus_tag="WHT44_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ2534085.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            1692..1817
                     /locus_tag="WHT44_001960"
                     /pseudo
     CDS             1692..1817
                     /locus_tag="WHT44_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            1857..>2218
                     /gene="mafB"
                     /locus_tag="WHT44_001961"
     CDS             1857..>2218
                     /gene="mafB"
                     /locus_tag="WHT44_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ2534086.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNARGSVKNRVYAVQTFDATAVSPVLPITHERTGFEGVIGYETHFSGHGHE
                     VHSPFDHHDSKSTSDFSGG"
BASE COUNT          757 a          412 c          471 g          578 t
ORIGIN      
        1 atttaggaaa tttaagtccc gaagcacaac ttgcggctgc aaccgcatta caagacagtg
       61 cttttgcggt aaaagacggt atcaactctg ccaaacaatg ggctgatgcc catccgaata
      121 taactgcaac agcccaaact gcccttgccg tagcagaggc cgcaactacg gtttggggcg
      181 gtaaaaaagt aaaacttaac ccgaccaaat gggattgggt taaaaatacc ggctataaaa
      241 aacctgctgc ccgccatatg cagactgtag atggggagat ggcagggggg aatagaccgc
      301 ctaaatctat aacgtccaac agcaaagcag atgcttccac acaaccgtct ttacaagcgc
      361 aactaattgg agaacaaatt agtagtgggc atgcttataa caagcatgtc ataagacaac
      421 aagaatttac ggatttaaat atcaattcac cagcagattt tgctcggcat attgaaaata
      481 ttgttagcca tccaacaaat atgaaagagt tacctcgcgg tagaactgcg tattgggatg
      541 ataaaacagg gacaatagtt atccgagata aaaattctga cgatggaggt acagcattta
      601 gaccaacatc aggtaaaaaa tattatgatg atttatagga aaaagccatg aatatactat
      661 ccataaataa tcaaaactca actatttcac taactcaaga tgaagttttt gttttacgag
      721 ctatcttgaa tgagatatat gcgggcgtat gtgtagattc aagagaattt gaaaatgtat
      781 ctggtgttag aaaacatgaa gtagataatt tacaacaaca gtttgctgga atttataaaa
      841 aaatgacaac ttaacaaccc aaatttttgt cagagcctag tgcaaattac aactatgatt
      901 ctcttgtagc tgcaatgaac gaaaaaaaat catgggttgg cgacagggtt gatgttgtta
      961 atatgcctga tggagcacct actagtatgg ataacacgcg tattatggca gcacgtgaag
     1021 caggggtaaa agtggaagcg aatgttcata attttaatga ccgattatca tcaaaagaga
     1081 gaatcaggtt taagcatgat ggtattgagc ctcaaacttg gggagaagct atccagctac
     1141 gaattagaaa gcaagaaaca caaaaaggag ttccagaagg gtggagcaaa agatttccta
     1201 acggaagtat ttatgatgta aaggtactta ggaaatgata aaacaaaata gttttgttcc
     1261 gtatcctgaa gcaatgcttc ctaaaggatt taaatatccg caaagttatt taaaattagc
     1321 tcaatccact catgccatta actacgatga acaatattct tttccttggt ggtttgaaaa
     1381 tgcagaaagc aatatatcag aagtaattga catttatttt gaaataactg gcattccaaa
     1441 cctattacct tttgctagaa accaagagtg ggctgcctgt tttgatattt cagataaatc
     1501 aggtaatcct aaaattatag tagttaattt agataataca aaatattacg agacttttga
     1561 aaattttgat acttggctaa aagaagctga aaatgatggt tggtagcagc cgtaggtcgg
     1621 attctcgaat ccgacatttt ccaacagcgg catttcggaa acgatagacg cgtcaaatat
     1681 ttttgtcgga tacaagtatc cgaccatacg gcaatcatac gggtaactct gccccatccg
     1741 tagaggctga taacagtcat gaggggtatg gatacagcga tgaagcagtg cgacaacata
     1801 gacaagggca accttgattc acactaccat aaccgcttgc taccaaggaa aacaaaatga
     1861 atttgcctat tcaaaaattc atgatgctgt ttgcagcagc aatatcgttg ctgcaaatcc
     1921 ccattagtca tgcgaacggt ttggatgccc gtttgcgcga tgatatgcag gcaaaacact
     1981 acgaaccggg tggtaaatac catctgtttg gtaatgctcg cggcagtgtt aaaaatcggg
     2041 tttacgccgt ccaaacattt gatgcaactg cggtcagtcc tgtactgcct attacacacg
     2101 aacggacagg gtttgaaggt gttatcggtt atgaaaccca tttttcaggg cacggacatg
     2161 aagtacacag tccgttcgat catcatgatt caaaaagcac ttctgatttc agcggcgg
//
LOCUS       DAWGRZ010000219         2207 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.219, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000219 DAWGRZ010000000
VERSION     DAWGRZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.219"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            32..516
                     /locus_tag="WHT44_001962"
                     /pseudo
     CDS             32..516
                     /locus_tag="WHT44_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            612..1235
                     /gene="rsmG"
                     /locus_tag="WHT44_001963"
     CDS             612..1235
                     /gene="rsmG"
                     /locus_tag="WHT44_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ2534087.1"
                     /translation="MERKERLRAGIAAMGLDISETAQDRLLVYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQEEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            1334..2107
                     /locus_tag="WHT44_001964"
     CDS             1334..2107
                     /locus_tag="WHT44_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ2534088.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVEEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELAARVSGK"
BASE COUNT          432 a          505 c          768 g          502 t
ORIGIN      
        1 atgcggtctg cttcggaaga gggggacggc gatgtttgtg aacgagaaat atccttatgc
       61 ggctctgttt gcgggactgg tgtttttgac gctgccgttt gcgttggcgg tgcatgatgc
      121 ctttgcgctt gcgttcggac ggacggggtt gctggtgtcg gtgtcggacg gcggattcgg
      181 ctggcgtggc ggttgggacg gcactgtttg gtttgtgttc ggtgtgtttg cgtttttgaa
      241 tgtggttgtg tcggcgggtc tgacgaaact ggcgtacaaa aagatgatgc ggcggcattc
      301 gcgttacaca ctgtttctgt cgggcgtggc ggcttgcgcg gcggcagcgg tggcttggat
      361 tttcgagctg ctgcttggca gtggggcttt gggcgggctg cgggggaggc ggtgttggaa
      421 tatgcgtttg ccgtgtggct ggtggcgatg ctgacgctgc ccaaacgcct gacgcgcgcg
      481 ccggtgcagc cggtggtgtt tcacaggaaa aaataggttg gaacgggaaa tgccgtctga
      541 aacccgacac gcggtttcag acggcatgtt tttccgctaa cattacgcct gaatatggac
      601 aggaagcaga tatggaacgc aaagaacgcc tgcgtgcagg cattgccgcg atggggctgg
      661 atatttcgga aacggcgcag gacaggcttt tggtctatgt ggatttgttg aaaaagtgga
      721 acaaaaccta caatctgacc gccctgcgcg acgaggaaaa aatgattgtc catcatcttt
      781 tggacagcct gacgctgctg ccccatatcg agggtgtgca aacgatgctg gatgtcggtt
      841 cgggcggcgg tcagcccggc attccggcgg cggtgtgccg tccggatgtg caaataaccc
      901 ttttggatgc gaatacgaag aaaacggctt ttttacagca ggcggttatc gagttggggt
      961 tggacaatgt gcgcgtggta tccggacgcg tggaggcggt ttcggacgtg cgtgccgatg
     1021 tggttaccag ccgtgcgttt gcagaactgg cggattttgt gtcgtggacg gtgcatctgt
     1081 tgaaagacgg cggctactgg gcggcgatga agggcgtgta tccgcaggaa gaaatcggcc
     1141 gcctgccgca ggatgtgtgc gttgaaaaag tccaaaggct cgacgtgccg ggcttggatg
     1201 cggaacgcca tatcgtcatc ctgagcaagc gttgagcgca cttcagacgg catgaatacc
     1261 ttttttgtgc ggataaaggt aaaattccgc actgtttttc ttttttcaac atcagacggg
     1321 acacgggcgg gacatgagtg cgaacatcct tgccatcgcc aatcagaagg gcggtgtggg
     1381 caaaacgacg acgacggtaa atttggcggc ttcgctggca tcgcgcggca aacgcgtgct
     1441 ggtggtcgat ttggatccgc agggcaatgc gacgacgggc agcggcatcg acaaggcggg
     1501 tttgcagtcc ggcgtttatc aggtcttatt gggcgatgcg gacgtgcagt cggcggcggt
     1561 acgcagcaaa gagggcggat acgctgtgtt gggtgcgaac cgcgcgctgg ccggcgcgga
     1621 aatcgaactg gtgcaggaaa tcgcccggga agtgcgtttg aaaaacgcgc tcaaggcagt
     1681 ggaagaagat tacgacttta tcctgatcga ctgcccgcct tcgctgacgc tgttgacgct
     1741 taacgggctg gtggcggcgg gcggcgtgat tgtgccgatg ttgtgcgaat attacgcgct
     1801 ggaagggatt tccgatttga ttgcgaccgt gcgcaaaatc cgtcaggcgg tcaatcccga
     1861 tttggacatc acgggcatcg tgcgcacgat gtacgacagc cgcagcaggc tggttgccga
     1921 agtcagcgaa cagttgcgca gccatttcgg ggatttgctt tttgaaaccg tcatcccgcg
     1981 caatatccgc cttgcggaag cgccgagcca cggtatgccg gtgatggctt acgacgcgca
     2041 ggcaaagggt accaaggcgt atcttgcctt ggcggacgag ctggcggcga gggtgtcggg
     2101 gaaataggtc aatccaaatc gggctgcccg tgcctttatg ctgtttggcc gggtgcgtta
     2161 tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagt
//
LOCUS       DAWGRZ010000220         2182 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.220, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000220 DAWGRZ010000000
VERSION     DAWGRZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.220"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            239..>2182
                     /locus_tag="WHT44_001965"
     CDS             239..>2182
                     /locus_tag="WHT44_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ2534089.1"
                     /translation="MADGYWALGLGIEAERIHNEQAVNNPNGSERDNRKQLISALDKG
                     FDGSFKEKHFTFLQSVIMDVTKLGVEYTIDGWQKIGGWGNGIINDLYKSVVKREWTGI
                     FEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKETKKRNGKYHVYDPLALDLDGDGI
                     ETVAAKGFSGSLFDHTNNGIRTATGWVSADDGLLVRDLNGNGIIDNGAELFGDNTKLA
                     DGSFAKHGYAALAELDSNGDNIINAADAAFQSLRVWQDLNQDGISQTNELRTLEELGI
                     QSLDLAYKDVNKNLGNGNTLAQQGSYTKTNGTTAKMGDLLLAADNLHSRFKDKVELTA
                     EQAKAANLAGIGRLRDLREAAALSGDLANMLKAYSAAETKEAQLALLDNLIHKWAETD
                     SNWGKKSPMRLSTDWTQTANEGIALTPSQVAQLKKNALVSLSDKAKATIDAARDRIAV
                     LDAYTGQDSNTLYYMSEEDALNIVKVTNDTYDHLAKNIYQNLLFQTRLQPYLNQISFK
                     MENDTFTLDFSGLVQAFNHVKETNPQKAFVDLAEMLAYGELRSWYEGRRLMTDYVEEA
                     KKAGKFEDYQKVLGQETVALLAKTSGTQADDILQNVGFGHNKNVSLYGNDGNDTLIGG
                     AGNDYLEGGSGSDTYVFGKGFGQD"
BASE COUNT          678 a          446 c          511 g          547 t
ORIGIN      
        1 gatgaaaaaa ggtgtggaaa acctatggga tgatacggtc ggtaaaaaga cccgtttaga
       61 gaaatttgat cgggttgcat tgcaacattt cagccaatat gtagatctaa ttaatgaaaa
      121 taatggtaga ttacctaaca ctagtgaaat tgagagaagt tactataaag ccgttaccga
      181 aaatggtgtt tcttctagtg cagctattga tttagttatt aatcgctcac ttccggatat
      241 ggcagatggt tattgggcat taggtttggg gatagaagcc gaacgtatcc acaatgagca
      301 agcagtaaat aatccgaacg gtagcgaaag ggataataga aagcagttaa tatctgcttt
      361 agataaagga tttgatggat cttttaaaga gaagcatttt acttttttac aatctgtgat
      421 aatggatgta acaaagttag gtgttgaata tacaatagat ggttggcaaa aaattggagg
      481 ttggggtaat gggataatca atgatttata taaaagtgtt gtaaaaagag agtggactgg
      541 aatatttgag atcgttaata ataacatcaa gcaatttaga gatctgttcc caaatccgga
      601 aggctggatc gatgatggtc accaatgttt cgctccttgg gttaaagaaa ctaaaaaacg
      661 caatggcaaa tatcatgtct acgaccccct tgccctagat ttggacggag acggcataga
      721 aactgtcgct gccaaaggct tttcaggcag cttatttgat cacaccaaca acggtatccg
      781 caccgccacc ggttgggttt ctgccgatga cggtctgctt gtgcgcgatt tgaacggcaa
      841 cggcatcatc gacaacggtg cggaactctt cggcgacaat accaaactgg cagacggttc
      901 ttttgccaaa cacggctacg cggctttggc cgaattggat tcaaacggcg acaacatcat
      961 caacgcggca gacgccgcat tccaatccct gcgtgtatgg caggatctca accaggacgg
     1021 catttcccaa accaacgaac tccgcaccct tgaagaattg ggtattcaat ctttggatct
     1081 cgcctataaa gatgtaaata aaaatctcgg taacggtaac actttggctc agcaaggcag
     1141 ctacaccaaa acaaacggta caaccgcaaa aatgggggat ttacttttag cagccgacaa
     1201 cctgcacagc cgcttcaaag acaaagtgga actcactgcc gaacaggcaa aagccgccaa
     1261 tcttgcgggc attggtcgtc tgcgcgattt gcgcgaagct gccgcattgt ccggcgattt
     1321 ggccaatatg ctgaaagctt attctgccgc cgaaactaaa gaagcacagt tggcattgtt
     1381 agataatttg attcacaaat gggcggaaac cgattcgaac tggggcaaaa aatcgccaat
     1441 gcgactttca accgattgga cgcaaacggc taatgaaggt attgcactga caccatccca
     1501 agtagcacaa ctaaaaaaga acgctttagt ttccctttct gataaagcta aagcaactat
     1561 tgacgccgcc cgcgaccgca ttgccgtgct tgatgcctac acggggcagg attccaacac
     1621 actctattac atgagcgagg aagatgcgct taatatcgtc aaagtaacca acgatacata
     1681 cgaccatctc gccaaaaaca tctaccaaaa cctgttgttc caaacccgtt tgcagccata
     1741 tttgaatcaa atcagtttca aaatggaaaa tgatacgttc actttggatt ttagtggtct
     1801 tgttcaagca tttaaccatg tcaaagaaac taatccgcaa aaagcttttg tggatttggc
     1861 cgagatgctt gcatatggcg aacttcgttc ttggtatgaa ggccgaagac taatgaccga
     1921 ttatgtggag gaggcaaaaa aagcaggtaa atttgaagat taccagaaag tgttgggtca
     1981 ggagaccgtt gcattattag ctaaaacatc gggtacgcaa gcagatgata tcctgcaaaa
     2041 tgtaggcttt ggtcataata aaaatgtttc tttatatggt aatgacggca acgacactct
     2101 gatcggcggt gcaggcaatg attacttgga gggcggcagc ggttcggata cttatgtctt
     2161 cggcaaaggc ttcggtcagg at
//
LOCUS       DAWGRZ010000221         2122 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.221, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000221 DAWGRZ010000000
VERSION     DAWGRZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.221"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            123..1508
                     /locus_tag="WHT44_001966"
     CDS             123..1508
                     /locus_tag="WHT44_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ2534090.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDILVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            1528..2046
                     /locus_tag="WHT44_001967"
     CDS             1528..2046
                     /locus_tag="WHT44_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ2534091.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
BASE COUNT          446 a          646 c          571 g          459 t
ORIGIN      
        1 ttcaaataaa caggaatata acttcagaca aacaacttac cgcccgattt gtgctatcgt
       61 tttcgcacaa cttaaaaaaa cctgacaatt ttgtactttt attacagaga aaggctttac
      121 aaatggacgg ctggacacag acgctgtccg cgcaaaccct gttgggcatt tcggcggcgg
      181 caatcatcct cattctgatt ttaatcgtca aattccgcat ccacgcgctg ctgacactgg
      241 tcatcgtcag cctgctgacg gctttggcaa ccggtttgcc cacaggcagc attgtcaacg
      301 acatactggt caaaaacttc ggcggcacgc tcggcggcgt ggcgcttctg gtcggcctgg
      361 gcgcgatgct cggacgtttg gtcgaaacat ccggcggcgc acagtcgctg gcggacgcgc
      421 tgatccggat gttcggcgaa aaacgcgcac cgttcgcgct gggcgttgcc tcgctgattt
      481 tcggcttccc gattttcttc gatgccggac taatcgtcat gctgcccatc gtgttcgcca
      541 ccgcacggcg catgaaacag gacgtactgc ccttcgcgct tgcctccatc ggcgcatttt
      601 ccgtcatgca cgtcttcctg ccgccccatc cgggcccgat tgccgcttcc gaattttacg
      661 gcgcgaacat cggccaagtt ttgattttgg gtctgccgac cgccttcatc acatggtatt
      721 tcagcggcta tatgctcggc aaagtgttgg ggcgcaccat ccatgttccc gttcccgaac
      781 tgctcagcgg cggcacgcaa gacaacgacc tgccgaaaga acctgccaaa gcaggaacgg
      841 tcgtcgccat catgctgatt cccatgctgc tgattttcct gaataccggc gtatcggccc
      901 tcatcagcga aaaactcgta agtgcggacg aaacctgggt tcagacggca aaaataatcg
      961 gttcgacacc gatcgccctt ctgatttccg tattggtcgc actgtttgtc ttgggacgca
     1021 aacgcggcga aagcggcagc gcgttggaaa aaaccgtgga cggcgcactc gcccccgtct
     1081 gttccgtgat tctgattacc ggcgcgggcg gtatgttcgg cggcgttttg cgcgcttccg
     1141 gcatcggcaa ggcactcgcc gacagcatgg cggatttggg cattcccgtc cttttgggct
     1201 gtttccttgt cgccttggca ctgcgtatcg cgcaaggttc ggcaaccgtc gccctgacca
     1261 ccgccgccgc gctgatggct cctgccgttg ccgccgccgg ctttaccgac tggcagctcg
     1321 cctgtatcgt attggcaacg gcggcaggtt cggtcggttg cagccacttc aacgactccg
     1381 gcttctggct ggtcggccgt ctcttggaca tggacgtacc gaccacgctg aaaacctgga
     1441 cggtcaacca aaccctcatc gcactcatcg gctttgcctt gtccgcactg ctgttcgcca
     1501 tcgtctgaca gacggaaagg atagtaaatg actacgcatt ttgtcgttat gggcgtatgc
     1561 ggctgcggca agaccaccgc cgcgctgtcc ctgcagaaac acctcggtca atgtccctat
     1621 gccgaaggcg acgagttcca cacccaagcc aaccgcgaca agatgggcgc gggtattccg
     1681 ctgaccgatg aagaccgcta tccgtggttg ggcaatctgc gcgactggat gacgcaacag
     1741 gcgcaaaacg gtgcgaacca caccatcgta acctgttccg ccctcaaacg cggctaccgc
     1801 gacattctgc gcggagccga aggcaaagct gccttcatcc acctcagtcc gccgcaagac
     1861 atcaacctcg agcgcatgat gtcgcgcaaa ggacattaca tgaaagcagg gatgctcgat
     1921 tcgcaactgg aaatcctcga ggaactgggc gaaggcgaat acggggtcaa aatcgccaac
     1981 cccggcacgc ccgaagcggt cgaagccgat attctgaact gggttgcctc ggaaaacctg
     2041 ctttgaagca atatgccgtc tgaagcccga cacaggatgg gtttcagacg gcataaacat
     2101 cgggaacaga atggattaca tt
//
LOCUS       DAWGRZ010000222         2095 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.222, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000222 DAWGRZ010000000
VERSION     DAWGRZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.222"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            234..2009
                     /gene="nhhA"
                     /locus_tag="WHT44_001968"
     CDS             234..2009
                     /gene="nhhA"
                     /locus_tag="WHT44_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin NhhA"
                     /protein_id="HEZ2534092.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVKTAVLATLLF
                     ATVQASANNEEQEEDLYLDPVQRTVAVLIVNSDKEGTGEKEKVEENSDWAVYFNEKGV
                     LTAREITLKAGDNLKIKQNGTNFTYSLKKDLTDLTSVGTEKLSFSANGNKVNITSDTK
                     GLNFAKETAGTNGDTTVHLNGIGSTLTDTLLNTGATTNVTNDNVTDDEKKRAASVKDV
                     LNAGWNIKGVKPGTTASDNVDFVRTYDTVEFLSADTKTTTVNVESKDNGKKTEVKIGA
                     KTSVIKEKDGKLVTGKDKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQTG
                     QADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVMYDVNVGDALNVNQLQNSGWNL
                     DSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMTPQFSSV
                     SLGAGADAPTLSVDGDALNVGSKKDNKPVRITNVAPGVKEGDVTNVAQLKGVAQNLNN
                     RIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDGGN
                     WIIKGTASGNSRGHFGASASVGYQW"
BASE COUNT          653 a          503 c          525 g          414 t
ORIGIN      
        1 ccgccgtcat tcccgcaaaa gcgggaatcc agacccgtcg gcacggaaac ttaccgaata
       61 aaacagtttc cttagattcc acgtcccaga ttcccgcctt cgcgggaatg acgagatttt
      121 aagttggggg aatttatcag aaaaccccca acccccaaaa accgggcgga tgccgcacca
      181 tccgccccca aaccccgatt taaccattca aacaaaccaa aagaaaaaac aaaatgaaca
      241 aaatataccg catcatttgg aatagtgccc tcaatgcctg ggtcgtcgta tccgagctca
      301 cacgcaacca caccaaacgc gcctccgcaa ccgtgaagac cgccgtattg gcgacactgt
      361 tgtttgcaac ggttcaggca agtgctaaca atgaagagca agaagaagat ttatatttag
      421 accccgtaca acgcactgtt gccgtgttga tagtcaattc cgataaagaa ggcacgggag
      481 aaaaagaaaa agtagaagaa aattcagatt gggcagtata tttcaacgag aaaggagtac
      541 taacagccag agaaatcacc ctcaaagccg gcgacaacct gaaaatcaaa caaaacggca
      601 caaacttcac ctactcgctg aaaaaagacc tcacagatct gaccagtgtt ggaactgaaa
      661 aattatcgtt tagcgcaaac ggcaataaag tcaacatcac aagcgacacc aaaggcttga
      721 attttgcgaa agaaacggct gggacgaacg gcgacaccac ggttcatctg aacggtattg
      781 gttcgacttt gaccgatacg ctgctgaata ccggagcgac cacaaacgta accaacgaca
      841 acgttaccga tgacgagaaa aaacgtgcgg caagcgttaa agacgtatta aacgctggct
      901 ggaacattaa aggcgttaaa cccggtacaa cagcttccga taacgttgat ttcgtccgca
      961 cttacgacac agtcgagttc ttgagcgcag atacgaaaac aacgactgtt aatgtggaaa
     1021 gcaaagacaa cggcaagaaa accgaagtta aaatcggtgc gaagacttct gttattaaag
     1081 aaaaagacgg taagttggtt actggtaaag acaaaggcga gaatggttct tctacagacg
     1141 aaggcgaagg cttagtgact gcaaaagaag tgattgatgc agtaaacaag gctggttgga
     1201 gaatgaaaac aacaaccgct aatggtcaaa caggtcaagc tgacaagttt gaaaccgtta
     1261 catcaggcac aaatgtaacc tttgctagtg gtaaaggtac aactgcgact gtaagtaaag
     1321 atgatcaagg caacatcact gttatgtatg atgtaaatgt cggcgatgcc ctaaacgtca
     1381 atcagctgca aaacagcggt tggaatttgg attccaaagc ggttgcaggt tcttcgggca
     1441 aagtcatcag cggcaatgtt tcgccgagca agggaaagat ggatgaaacc gtcaacatta
     1501 atgccggcaa caacatcgag attacccgca acggtaaaaa tatcgacatc gccacttcga
     1561 tgaccccgca gttttccagc gtttcgctcg gcgcgggggc ggatgcgccc actttgagcg
     1621 tggatgggga cgcattgaat gtcggcagca agaaggacaa caaacccgtc cgcattacca
     1681 atgtcgcccc gggcgttaaa gagggggatg ttacaaacgt cgcacaactt aaaggcgtgg
     1741 cgcaaaactt gaacaaccgc atcgacaatg tggacggcaa cgcgcgtgcg ggcatcgccc
     1801 aagcgattgc aaccgcaggt ctggttcagg cgtatttgcc cggcaagagt atgatggcga
     1861 tcggcggcgg cacttatcgc ggcgaagccg gttacgccat cggctactcc agtatttccg
     1921 acggcggaaa ttggattatc aaaggcacgg cttccggcaa ttcgcgcggc catttcggtg
     1981 cttccgcatc tgtcggttat cagtggtaag ggctttttcg cctgtctgct gttgggacag
     2041 gcggaaggtt tgaagggaag ggtggcgatt tgccgcctga gacctttgca aaatc
//
LOCUS       DAWGRZ010000223         2074 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.223, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000223 DAWGRZ010000000
VERSION     DAWGRZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.223"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            340..852
                     /locus_tag="WHT44_001969"
     CDS             340..852
                     /locus_tag="WHT44_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ2534093.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            932..2011
                     /gene="aroB"
                     /locus_tag="WHT44_001970"
     CDS             932..2011
                     /gene="aroB"
                     /locus_tag="WHT44_001970"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ2534094.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGALGDIGFFEWLEQHMPELMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          508 a          655 c          510 g          401 t
ORIGIN      
        1 aaggcgaggc aacgccgtac tggtttttgt taatccacta tagtattgat aaacatatta
       61 tcttcaatat attcaattgg ataattgttt acctaagcaa agataattgc cttttcctga
      121 caaataagtg aaatcaacgg attgtcaaaa cacagcctga aataaaaaac ctccctgatt
      181 tcttttattt gtccttaaaa tcagaaaggt tcgggatggt cgggttattt ttccaaacgt
      241 accgccgccc tgccgatttc gtataaaatt ccgccgtaac ccgacaagcc cgaaccctgt
      301 cgccccgaaa ggcggggtgt caaacattaa ggaattgtga tgaaaaactt taacggcaaa
      361 ctcatcctca tcggactgat gggcgcgggc aaaaccacgc tgggccggca aatggcgcag
      421 cggctggatt accgttttta cgacagcgat cacgaaatcg ccgcagccgc cggcgttccc
      481 atccccacca tatttgaaat ggaaggcgaa cagggattcc gttcgcgcga aaccgccata
      541 ctcaaaaagc tggttatcct gccccatatc gtcctgtcca ccggcggcgg cgcggtgtta
      601 aaagaagaaa accgcgccct tatccgcaaa agcggcacgg tcgtctatct gcacgccccg
      661 cccgaaaccc tgctcgaacg cacgcgctgc gacaacagcc gtcctttgct gcaagttgcc
      721 gatcctttgg cgaaattacg tgaactctac gccgcacgcg accccgttta ccgccaaacc
      781 gccgacttta ccgtagaatc ggcaaactgc cgggaaaccg tgcaaaccct gctcaaacgc
      841 ttatcccgat aaaccggcat atgcgccgcg cccagaaaac caaaccgcgc cccgcccggc
      901 gggccggcgg ttcaaacttt aaggaacaac aatgaaaaca ctgaccgtac acaccccgtc
      961 ccacagctac cccatcttta tcggcaacgg actgctgccg caggcaggaa gcctgctcaa
     1021 accgcatttg ggcaaacgcg ccgccatcat cgccaacgaa accgtcgccc cgctctacct
     1081 cggcacgctt cagacggcat tggatgcggc aggcgtatcc catttcagca tcatcctgcc
     1141 cgacggcgag gcgcacaaaa actggcagac gctcaacctc atctttgacg ggctgatgca
     1201 aaaccgcgcc gaacgcaaaa ccacattaat cgcactgggc ggcggcgtga tcggcgacat
     1261 ggtcggcttt gccgctgcca cctaccagcg cggcgcaccg ttcgttcaaa taccgaccac
     1321 gctgttgagt caggtcgact catcggtggg cggcaaaacc gccatcaacc acccgctcgg
     1381 caaaaatatg attggcgcgt tttaccagcc gcaggcggtg cttgccgact tggacacgct
     1441 gcacaccctg cccgcccgcg aattgtccgc cggtatggcg gaagtcatca aatacggcgc
     1501 gctcggcgac atcggctttt ttgaatggct ggaacagcat atgcccgaac tgatgacgct
     1561 cgatcgggaa aaactcgccc aagccgtgta ccgctgctgc caaatgaagg cagacatcgt
     1621 cgcccaagac gaaaccgaac agggcatacg cgcatggctc aacctcggac acaccttcgg
     1681 acacgccatt gaaaccgaga tgggttacgg cacttggctg catggagaag ccatcgccgc
     1741 cggctgcgtg ttggcggcgc gtttgtccga acaactgggc aaaacctccg ccgcagatac
     1801 cgcgcggctc gccgccctgc tcgaagccgc cggactgccg tccgcgccac ccgtgtttgc
     1861 ctttgaaaaa tggctggaac acatgagcca cgataaaaaa gtcagcggcg gcatcatgcg
     1921 ctttatcggt ctgaaccggc tgggcgaagc caacatcacc gaaattaccg acacggacat
     1981 cctccgccgc accctgcaac cgtatctctg atttcctctg ccgatgtgct gccgcgcggg
     2041 tttgacgcac gatgatgatt ttccatcatc tttc
//
LOCUS       DAWGRZ010000224         1986 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.224, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000224 DAWGRZ010000000
VERSION     DAWGRZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.224"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            305..1762
                     /locus_tag="WHT44_001971"
     CDS             305..1762
                     /locus_tag="WHT44_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ2534095.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMMGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISSGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          453 a          592 c          456 g          485 t
ORIGIN      
        1 ctataatgcg cccttcggcg tggcggatat ataaggaagt gattttccat ctaagtaaaa
       61 accgccctat cggataagcc cttaacagaa aaggctttac ccgcgccgta tcggaacaca
      121 tcctctaaaa tacaatccgt tgaattgaaa aaaatataaa aacatccgcc cgcgaaaaac
      181 ggcagcgcgt cgtttgacaa agaatgaaaa tatcggttaa aaaccgattt tcatacaaaa
      241 aacaccgctg ccgtccgcat ccgtttcaga cggtattgag agaaaatctt ttaggagaac
      301 ctttatgtcc cggcatcccg cccccaccgg agaaaaaaca ttcttcggcc accccttcca
      361 gctttccacc ctcttccata tcgaattgtg ggaacgtttt tcattttacg gaatgcaggg
      421 catcctgctg atttacctct actacaccgc cgacaaaggc ggcttgggca tagacaaaac
      481 cctcgccggc ggcattgtcg gcgcatacag cggcagcgtg tacctgtcca ccattttggg
      541 ggcgtggttt gccgaccgag tatggggtgc ggaaaaaacc ctcttcctct cgggcatcgt
      601 cgtgatgctc ggacacatcg tccttgccgc cgccccgggc ctgtacggcc ttttaatcgg
      661 gctgatattc atcgcattgg gcagcggcgg cgtgaaatct acggccagtt ctatgatggg
      721 cgcattatac gaacaggacg aaatgcgccc gctgcgcgat gcgggatttt ccattttcta
      781 catcgccatc aacatcggcg gcttcctagg cccgctgctg accggcctac tgcaggaaaa
      841 catcggtttc cattatggtt tcggcgcggc ggcggtcggt atggcattcg gcttgtggcg
      901 ttattccctg ggacgtaaaa acctgcccca ccccaccgtc ccccatccgc tttcaaaagg
      961 acagggcaaa actgcggccg ccgtcggcat cgccctcatc gccgcacttg caaccgccat
     1021 caaaaccggg cttgtcaacc tcgacaattt ctccggcatc ctattatcta ccgtcatcct
     1081 tgccgtcatc gcctatttcg cccgcctgct gaccaacccc cgcgtcagtt ccgacaacaa
     1141 acggcacatc atcgcctaca tcccgctttt cctgaccatc tgtatgtttt gggccgtctg
     1201 gtttcagatt tacaccgtgg caaccgtcta tttcgacgaa accgtcaacc gcaccatcgg
     1261 ttcgtttacc gtgcccgtcg cttggaaaga ttctatgcaa agcctgtggg tcatcctgtt
     1321 ttccggactg atggcggcaa tgtggacaaa aatggggcgc aaacagccca aaaccccgct
     1381 gaaattcgct atggcggtat ttgttaccgg cgcgtcgttt ttgggattcg tcccctttat
     1441 ttcctccggt acgccgatgc ctattgcggt tttcgcactg atcgtcctcg ccatcacgat
     1501 aggcgaactg atgatttccc cgattgcgct gtccatctcc accaaaatcg caccgccttt
     1561 attcaaaacc caaatggtcg cccttaattt ccttgccttt tcattaggct tcactttggg
     1621 cggcgtattg tttgaaaaag gctatcaggc gggcgacgaa atcggcttct atcggctgct
     1681 gttctacatc ggcgcagcca caggcttcct gctgctcctg ctcgtcccca aattgaacaa
     1741 aatgctcgaa ggcacagact aagtcccgcc ccgatgccgt ctgaaccctt cagacggcat
     1801 ttttccgcat aatgaaacca aaccgtttcc acccgacagg acaggctccc gcccaaccgg
     1861 aaggcagcct gccgattgtc atttgaataa cgcaagggaa agccgttgat ttccgtttgt
     1921 atggaaacag tttggtttca ttggaaaaag gcattttgtc cgactaaatt agtgctgcat
     1981 caacga
//
LOCUS       DAWGRZ010000225         1893 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.225, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000225 DAWGRZ010000000
VERSION     DAWGRZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.225"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            385..1830
                     /gene="zwf"
                     /locus_tag="WHT44_001972"
     CDS             385..1830
                     /gene="zwf"
                     /locus_tag="WHT44_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ2534096.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERFAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQACENLAAIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
BASE COUNT          508 a          430 c          499 g          456 t
ORIGIN      
        1 tatctgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc actatatttt
       61 aaaaggtata ccgtctgaaa gtgggaagcg ggtgttatta tagtacaaaa tttgtaattt
      121 tgttacaaaa aaatcagaca tcgaaaagaa atttgattta aatcgcagaa tcttccgatt
      181 gttgtaacgg aataggggaa acctgcccga tttttggact aggtcaaata aattgtaaaa
      241 atgggaatag acaaaaagtg tagcggtgtg tagtattact actcattatg ccgcctgcct
      301 ttgtttccga tattgcgatg cagcgtgatg ggggaaacag gtttcagacg gcatcgaaaa
      361 attctttcct gacgagataa tacgatgagt acacaaacaa attttgattt ggtgttgttc
      421 ggtgcgaccg gcgatttggc gatgcgcaag ctgttgccct gtctgtacca ggcgcatgtt
      481 gccggtctgc ttcatcccga agggcgtatt ttgggggtca gccgcagcga gttggatacc
      541 gaaggttttc tggcgaaggt ggaaaccagt tccaaaatcc atgtgaaaga aaatttttcc
      601 gatgaggctt gggcatcgtt tgtcgaacgt ttcgcatatc tcaaagtcga tgttacgcag
      661 ccggacgatt ttgccgcttt gggcgatttg gtcaaagcgc gcaaagaaac cgacaatgtc
      721 gtcatctatc tttccaccgc gccgaaattc ttcgcgcaag cctgtgaaaa ccttgctgcg
      781 atcggtttga atgccgacaa tgtgcgcgta gttttggaaa aaccgttggg tacggatttg
      841 gcatcttccc aacaaatcaa taccgatgtt gcgcgttatt tcaaagaagg gcaaatctac
      901 cgtatcgacc attatttagg taaagaaagc ctgcaaaacc tgctggcctt gcgttttgcc
      961 aatgtgatgt tcgagccgct ttggaacaat aaatacatcg aaagcgtgca gctgaccatc
     1021 gccgagcagt tgggcgtgga agaacgcggc gagttttacg acattaccgg cgcgttgcgc
     1081 gatatggtgc aaaaccactt gatgcaaatg ctgtgcatga ctgcgatgga agcccccgcc
     1141 agcttggatg ccgacgcggt gcgcgatgaa aaagtcaaag tcatcaagtc attgaagccg
     1201 ctgaccgtcg aatctgtcaa tgaaaatgtc gtgcgcggac aatataccgc cgccaaaggc
     1261 atgaacggct atcttgaaga aatcaacgtt ccgcaagaca gctttaccga aacctacgtc
     1321 gccattaaag ccgaaatcga aaacgaacgc tggaagggcg ttcccttcta cctgcgtacc
     1381 ggcaagcgca tggcgggcaa agtggcggaa atcgttttga acttcaaaga tttgaacagc
     1441 catatttttg aaggcagtcg caccgcgccc aaccggctcg ttatcgagtt gcaaccatat
     1501 gaatccgtgc gcctctatac gcagatgaaa accccggggg caggaaataa ggtcgaaacc
     1561 gtgccgctgg caaccgattt gggcaaagca ttggaaagcc gccgcgcgga agcttacgag
     1621 cgcctgctgc tggatgtgat taacggcaaa ctcgctttgt ttaaccgccg cgacgaactt
     1681 gaagccgcgt gggaatatgt gatgccgatt ttggaaaact ggacaaataa caccacgccg
     1741 ccgcacggct atggcgcaca ctcgtggggg cctgaagccg cgcgcgaact attggcgcgc
     1801 gacggacaca agtggcacga agagcagtaa tacaataatg cgttcagacg gcatggggtt
     1861 tgaaatgccg tctgaacgta agtaaagcag tat
//
LOCUS       DAWGRZ010000226         1882 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.226, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000226 DAWGRZ010000000
VERSION     DAWGRZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.226"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(127..1437)
                     /locus_tag="WHT44_001973"
     CDS             complement(127..1437)
                     /locus_tag="WHT44_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ2534097.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYSA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEVLKAK"
BASE COUNT          449 a          481 c          494 g          458 t
ORIGIN      
        1 ttaatccact ataccacgtt gtctttattg gctttatcaa taaacaggat aagacctgaa
       61 aaaaagccga tacgcctttt tggtgtaccg gctttgccat actgttctgc ttcagacagc
      121 attgcttcat tttgccttta atacttcttc gtccagcgat ttcaaccatt ccagcttttc
      181 gccgattttg atttccaacc cgcgcgggac gggttggtag aagtccggtt cgtccaagcc
      241 gtcgggcata tagctttcgc cggcggagta ggcgttcggt tcgtcgtggg cgtagcggta
      301 ttcgcgtccg tagcccaatt ccttcatcag cttggtcggg gcgttgcgca ggtggacggg
      361 cacttcgtcg ctagcgtttt ctttgacgaa gtggcgcatt tggttgtatg ccttgtagcc
      421 cgcgttggat ttcgcggcgg cggcaagata caataccgct tgcgccaaag ccagttcgcc
      481 ttcgggcgag cctaagcgtt cgaaggtggc ggcggcatcg ttggcgattt ggaaggcgcg
      541 cgggtcggca agcccgatgt cttcccaagc gatacgcacg atgcggcggg cgaggtagcg
      601 cgggtcggtg ccgccgtcga gcatacggca gaaccaatac agcgcggcgt tcggatgcga
      661 accgcgcacg gatttgtgca gggcggagat ttggttgtag aaactctcgc cgcctttgtc
      721 gaaacggcgg atttgcgccc cgagactgtc ggcgagaaat tcggcggtta agtttttcag
      781 acgacgtgta tcggcggcgc gtaaaagttg ttccaacaaa ttcaacaatc tgcgcgcatc
      841 accgtcggcg gtattcacga gtaatttttg cgcatccgtt tcaatcgtaa actcttggta
      901 ttcaggcaaa gccaatacct tggcaatcag ctttttcagg tcgtctgaag acaagggttg
      961 caaaacatac acctgagcgc ggctcaacag cgcgggattg acttcaaacg acggattttc
     1021 cgtcgtcgca ccaataaagg ttagcaaacc gctttcgaca tgcggcaaaa acgcgtcctg
     1081 ctgcgccttg ttgaagcggt ggacttcatc gacaaacaaa atcgtcgcgc gtccctgctg
     1141 caaagcgatt tcggctttat cgattgcctc gcggatgtcc ttcacgccgg aaaatacggc
     1201 ggaaacaggc aaaaactggg cgttgaaact ctgcgccaaa atccgcgcca acgtcgtctt
     1261 gcccacgccc ggcggccccc acagcaacat agaatgcggc ttgccgcctt ctaccgccac
     1321 gcgcaaaggt ttaccttcgc cgatgaggtg ttcctgcccc accacgtcgt caagcgtatg
     1381 cggacgcaat cgttcggcaa gcggcgcgtc gggttctcgg gcaaacaaat cggtcataac
     1441 ggctccgtca acaggttttc aaacaatatg attatacggc agggaacggc ggcgtgccgc
     1501 atacggattc cgcccctccg tttgccttaa gccgatatta ggcgcatact ggaaaagacg
     1561 agagacttca cacaatatat ccggcacgga gaccgattcc gcatcggcat gacaataccc
     1621 aaatcagcgt ttcaattaaa cattaaggag actaaaatag aaaatttgct ttatctacca
     1681 ttgctttgtt gatttaatcg gcattatgtt ttgaggcgga agcccatgaa tatactaata
     1741 ttcaagagat ggaatgggtg tctttatttt ctgatccgca aagagacgat gatagtctta
     1801 taacccttaa agatgaaaaa atcactgtaa aaaactatat tgtgccttgg tggaaaaaag
     1861 gtgaaaactt tagaaaatta ga
//
LOCUS       DAWGRZ010000227         1871 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.227, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000227 DAWGRZ010000000
VERSION     DAWGRZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.227"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            226..1698
                     /gene="pyk"
                     /locus_tag="WHT44_001974"
     CDS             226..1698
                     /gene="pyk"
                     /locus_tag="WHT44_001974"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ2534098.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENALIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQVEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
BASE COUNT          477 a          543 c          508 g          343 t
ORIGIN      
        1 ttatctagga cagccccaaa ataaatttag cctaagtcat aaaaccgtgt aattttgcgc
       61 caaaaaaggc ggtttgcagg taaaatatca aactattaat ttaaatcata tttttacaga
      121 atattccgcc gcgttcatca aatggacatc aaaccggttc caaatttctg taattttgta
      181 acaaaatacc gcaaaacacc cgattgagac caaaaggact ttcatatgaa ccagacaagc
      241 cgcgatctga cccgcatcag ccacaacact aaaatcgtcg ccacccttgg gccgggcagc
      301 aacaacgtcg aactgttgga agacatgatc cgcgtcggcg gtctgaacgt cgtccgcttc
      361 aacttcagcc acggcacgcc cgaattccat caggaaaacg ccctcatcgt gcgcgaggcg
      421 gcaaaacgcg ccggacagga aatcgccatc attgccgacc tgcagggccc gaaaatccgc
      481 gtgggcaaaa tcgccggcgg cggcatcgaa ttgaacaaag gcgaaacgct ggtactggat
      541 gccgcgctcg aaggcgaagg cacgcgcgag gcggtcggtt tggactaccg cgacctgccc
      601 gacgacgttg ccgcaggcga tgtcttgtgg ctggacgacg gcctgctgac cctgaccgtg
      661 gaatccgtcg aaggcagcag gattatcaca agggtggaaa acagccacgt cctgaaaagc
      721 aacaagggca tcaacaaacg cggtggcggt ctgtccgcag gcgcgttgac cgaaaaagac
      781 ttccgcgacc tgaaaaccgc gattgccatc ggttgcgact acctcgccat cagctttgtg
      841 aaatccgccg aagatttgca tatcgcgcgc gccaaagtcg aagaagaaat gaagggcagc
      901 acggccgtgc gccccggttt ggtttccaaa atcgaacgcg tggaagcgat tgaaaacttg
      961 gacgaaatca tcctcgccgg cgacggcatt atggttgcgc gcggcgactt ggcggtcgaa
     1021 gtcggacacg ccgccgtccc cgccctgcaa aaacggatga tccgccgcgc ccgcgagttg
     1081 cgccgcttca gcattacggc gacgcaaatg atggaatcga tgattaccaa ccccgtaccg
     1141 acccgcgcgg aagtcagcga tgtggcaaac gcggtattgg acggtaccga tgcggtgatg
     1201 tgttccgccg aaaccgccgt cggcgcgtat ccgtttgaaa ccgtcagcca aatggcgatt
     1261 atctgcgcgg ctgcggaaaa agagcaggat tcgctcaacg gcgttgccga acaggtcgag
     1321 tatcccgaag cggtcagcac caacctggcg gttgccggcg gtgcggtcag cgtggcgcgc
     1381 gcggttcacg ccaaagccat cgtcgccctg accgaaagcg gttcgaccgc ctttgaaatc
     1441 agccgccaca acatcaccct gccgattttc gcgctgaccc cgagcgtttc cgcccaacgc
     1501 cgtatggcga tgtaccgggg cgtgcgcccg ctgattttgg caaccagtac cgaccacgac
     1561 acggcgttaa acgaagtcga aacgatgctg gtggaacaca acatcctgca ttccggcgac
     1621 caatacatca ttaccagcgg ttcgcaaatg cgcgaatccg gttcgaccaa cacactggaa
     1681 gtgctgcgcg tcaaataatc cgccctgagt ggaaaatgcc gtctgaagcc gatgcccgag
     1741 gcttcagacg gcattttttg cggcggcgcg gcggttcggg caaactctga aaaaattgtg
     1801 caaatccggc aacatcggat aaaatcgagt acctatacta aagcgaaaca aggcatttcc
     1861 gactgccttt t
//
LOCUS       DAWGRZ010000228         1811 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.228, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000228 DAWGRZ010000000
VERSION     DAWGRZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.228"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(58..381)
                     /locus_tag="WHT44_001975"
     CDS             complement(58..381)
                     /locus_tag="WHT44_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ2534099.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(369..608)
                     /locus_tag="WHT44_001976"
     CDS             complement(369..608)
                     /locus_tag="WHT44_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ2534100.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(731..1528)
                     /gene="truA"
                     /locus_tag="WHT44_001977"
     CDS             complement(731..1528)
                     /gene="truA"
                     /locus_tag="WHT44_001977"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ2534101.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIGQMRQAAALLVGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          405 a          430 c          519 g          457 t
ORIGIN      
        1 ccgtactggt ttaaagttaa tccactataa tgaacacaat ccattcagac tattcaatca
       61 ggcaaacatc tcctgcaata ctgcaaacag tttttcagcc gtactgttgt ctaaattgcc
      121 aagatgtttg accaatccgg ctttatccac agccctaatc tgttcgggca aaagcaaacc
      181 gtctttatcc tgaaagcgga cattgacgcg gaacggggca ggacggcttc cgctcgtcat
      241 gggaacgatc agcacagtct tgagatagtt gtgtatttca ggaggagaga ctacgacaca
      301 aggacgtgtc tttttgattt cgcttcctac ggtcgggtct aaggagacca gatagattcc
      361 gccgcgtact accatatcca ttctttatcc gcttcgtttt caatttcgga aaaaaaatgc
      421 tcctgctcgg tttcgacaag cattgcggca gcttctgccc atcccctgcg aacggtagga
      481 cagcttaaaa taatattgcc cttttcaact gtaacagcca agctgtctac tgcccctatt
      541 tgacccaata atgatttggg cagaatcacg ccttgcgagt ttcccatttt gcgtatgttg
      601 agaatcatat acgtaccata ttcacctgtt tatgtaataa caatgttagt accttgatga
      661 ggtagtgtca acatggaaaa agattgcctg acagtttgtc cgatttcaaa atctccgcga
      721 caagcatgtt ttaaagccat tcggggattt gggggcggat gatgccgtat gcctcgggat
      781 agtcgacgcc ggtcaggtaa agtccgtcgg gcatgaaggt cggcggggct ttgaggcggc
      841 tgcgttcttg aatcagtgcg gcgaagcctt cgacgctgag tctgccgctg ccgacataaa
      901 cgagcgcgcc catgatgttg cgtaccatgt ggtgcaaaaa ggcgttgccg tgcaaatcga
      961 ggcggacgag tcctgagctt tgggtaaggt cggcgcggta gatggttttg acgggggatt
     1021 ttgcttggca ttcggcggcg cggaagctgg agaagtcttg ttcgccgacc aataaggcgg
     1081 cagcctgccg catctgcccg atgtcgagtt tgaggtgtgt ccagcctgcc ctgtttttga
     1141 gcagggggga acggacgggg gcggattcga gcaggtagcg gtagtgccgt ccgtatgcgt
     1201 caaatcgtgc atgaaattcg ggggcgacct gtcgggcgtg caaaacggca atgccttcgg
     1261 gcaggtgggc atttacgccg cgcacccatg cctgttgggg acgggcggca gttgtgtcga
     1321 agtggacgac ttgggcggtg gcatgcacgc cggtgtcggt cctgccggca acggtggtgg
     1381 aaaccgcttc ccctgctatt tgggcgagcg cggtttccaa tgccgcctga acggtcggta
     1441 cgccgtcagc ctgtttctgc cagccgtaaa agcggctgcc gtcataggat agggttattg
     1501 cccagcgttg tttttgtgcg gtatccatcg gatttgggat tcggataaat gttcagactg
     1561 cattgtatcg cagattttgc agggaaacgg caaacgccca gggcgagcgg cgttgtttgg
     1621 ggagttgttg ggggggggat gcagttgcta cgaatcgcta tcctgtgaat ttaccctgtc
     1681 aggagtgccc gaatcgtcat tcccgcgcag gcgggaatct aggacgtaaa atctaaagaa
     1741 accattttat agtggattaa caaaaaccag tacagcgttg cctcgcttta gctcaaagag
     1801 aacgattctc t
//
LOCUS       DAWGRZ010000229         1794 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.229, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000229 DAWGRZ010000000
VERSION     DAWGRZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.229"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(71..1279)
                     /locus_tag="WHT44_001978"
     CDS             complement(71..1279)
                     /locus_tag="WHT44_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ2534102.1"
                     /translation="MSEAEYFSHFISNGNGKLLEIPQRRGRQDGVFIDWLSFTLHEDS
                     LLKVSGCPLVSDAEYMFVLSKKLEEILGFGITSRCKSKGNKFYDSMFRLGSEEVDYGE
                     VHYGGQRNTVLIELKGVGCNIANPGWELRLKQFLEDSLRPRITRVDLALDFFDGEYTP
                     EQALLDHDNGFFDNSNMRPKSEMVGTAWRREDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNHGDMEIPLDILINQGSYFSGAFPICQKFKNMPNPERFDYRKKV
                     ANLTFQHKLRYAKNAVGKLINFMFDMGFDSDEIVRYLKADLGYPKGLEPEKYSLAGLK
                     ESLKFGFIHEQPDVDLEVELEELGIIKFKQSDKFDPDKRLFDPHHDVESERQYQLYLD
                     RMYDLHANQN"
BASE COUNT          513 a          429 c          301 g          551 t
ORIGIN      
        1 gctcctaaaa aagttgcggg ataagttaca gtttgagttt gattaaacat attaattttt
       61 cctttttagg ttaattttga tttgcatgaa gatcatacat tctgtcgaga taaagctgat
      121 attgcctctc actttctaca tcgtgatgtg gatcgaaaag ccttttatcc ggatcgaatt
      181 tatctgattg cttaaatttg ataattccga gttcttccaa ttcaacctct aaatctacat
      241 ccggttgttc gtggataaag ccgaatttca aagattcctt caatccggcc aacgaatatt
      301 tttcaggttc tagccctttg ggatacccca aatctgcctt cagatatctg acaatttcat
      361 cactatcaaa acccatatca aacatgaaat taatcagttt gccgaccgcg ttttttgcgt
      421 atctcaattt atgctgaaaa gttaaattag ccactttttt acggtaatcg aacctttccg
      481 gattcggcat atttttaaat ttctgacaaa tcgggaaagc gcctgaaaag taagaacctt
      541 gatttatcag aatatccaaa ggtatttcca tatctccatg attaaactga atttcgaacc
      601 ttacccactt gctttcttta tcgcctagct gcctgccttt ctcataaaca cgcacaaaac
      661 gagaattttt cttgcgacct acataaaatg tcttgccgct cccgtcctct ctccgccaag
      721 ccgttccaac catttcagat ttcggcctca tgttactgtt atcgaaaaaa ccgttatcgt
      781 gatccaaaag tgcctgttcc ggcgtgtact ccccatcaaa aaaatcaagt gccaaatcta
      841 cccgcgttat cctcggcctc aatgaatctt ccaaaaactg cttaagcctc aattcccaac
      901 ctggatttgc aatgttgcaa cctacacctt tcaattcgat taaaaccgta tttcgctgac
      961 ctccgtaatg gacttcgccg tagtcaactt cttccgatcc caacctaaac atcgaatcgt
     1021 aaaatttatt gcccttcgat ttgcatctgc tcgtgatgcc aaaccctaat atttcctcca
     1081 attttttgct taaaacaaac atatattcgg catcggaaac taaggggcat ccggaaactt
     1141 tcagcaagga atcttcgtgc agtgtgaatg acaaccaatc tataaaaacg ccgtcctgcc
     1201 tgcccctacg ttgcggaatt tctaataact tcccattgcc gttagatatg aaatgggaaa
     1261 aatattctgc ttcactcatt ttgttcagta cctttaggga tttgttttat ttcgctcccc
     1321 cccctgttag tcaggggggg ggggctttca gccgtttccc gtctgccgcg ctaaagcgcg
     1381 tccaacggtc aacgaccgaa agcccaatcc tgacaaactg ttaaagatca agaagaaaga
     1441 ccacaaccgt ctgttgtgat aattaccgga aaattcgagc caaccgaatc tatataatcg
     1501 aacgcctgat aaagctttga aaaattttct tgttcagcga gtttatgcgg ttcaccatgc
     1561 ctgaactgat agaaacataa aacgcaataa tctgattttt taaatattct ccaataggaa
     1621 caagaaaata ttacatttgc tactgacata aaaaagcccc tttcacttgg ctgtcaaagg
     1681 ggaatgttaa gaaaagtaat gcgccccttt gatagagcgc atcatataag gcgggaatcc
     1741 agtccgttca gtttcggtcg tttccgataa attcctgctg cttttcattt ctag
//
LOCUS       DAWGRZ010000230         1740 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.230, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000230 DAWGRZ010000000
VERSION     DAWGRZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.230"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            30..917
                     /gene="prmA"
                     /locus_tag="WHT44_001979"
     CDS             30..917
                     /gene="prmA"
                     /locus_tag="WHT44_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ2534103.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQTATQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEVPEGSAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGFAVGVDIDEQAVRAGKDNAAQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEEGWARLSGVKR"
     gene            935..1498
                     /gene="orn"
                     /locus_tag="WHT44_001980"
     CDS             935..1498
                     /gene="orn"
                     /locus_tag="WHT44_001980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ2534104.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDDVLNKMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
BASE COUNT          460 a          533 c          444 g          303 t
ORIGIN      
        1 aatccactat atttccaaga aagcccgtta tgccctacca acaaatcacc gtcaacgtca
       61 acgatgccgt cgccgaacgc ctcgccgacg cgctgatgga acacggcgca ctctccgccg
      121 ccatcgaaga tgcctacgcc ggcacgcaaa acgaacaggc gattttcggc gaacccggta
      181 tgcccgccga acaaatctgg cagcagagca aagtcatcgc cctgttcggc gaacacgacg
      241 aagccgccgc catcatccaa accgccacac aagaatgcgg gttaaaagac ttggcataca
      301 ccggcgaaac catcgaagac caagactggg tgcgtctcac gcaatcgcaa ttcgacccca
      361 tccggatttc cgaccgcctg tggattaccc cctcttggca cgaagtcccc gaaggcagtg
      421 ccgtcaacct ccgcctcgac cccggactcg ccttcggcac cggcagccac ccgaccacgc
      481 gcctctgcct caaatggttg gatacgcaac tcaaaaacgg cgaaagcgtc ctcgactacg
      541 gctgcggttc gggcatcctg accatcgccg ccctcaaact cggtgcaggt ttcgccgtcg
      601 gcgtggatat tgacgaacag gccgtccgcg ccggcaagga caacgccgcg caaaacaacg
      661 tcgatgcaca attcttcctg cccgacggtc tgcctcaagg gcaattcgac gtagttgtcg
      721 ccaacatcct cgccaaccct ttgcgtatgc ttggcgaaat gctcgccgcc cgcaccaaac
      781 agggcggacg catcgtgttg tccggtttgt tggacgaaca ggccgaagaa ctcggcggca
      841 tttacagcca atggttcgac ctcgacccgg cggaaaccga ggaaggatgg gcgcgattga
      901 gcggcgtaaa acgctgaaac ggaaaggaaa caccgtgcag gataaaaaca acctctgctg
      961 gctcgatatg gaaatgacgg ggctgaatcc cgaaaccgac cgcattatcg aagtcgcgat
     1021 gattattacc gactcggatt tgaatgtgtt ggcgcaatcc gaagtttacg ccgtccacca
     1081 aagcgacgac gtgctgaaca aaatggacga atggaacacc gccacacacg gcaggacggg
     1141 gctgacacag cgcgtacgcg aatcgtcgca taccgaagcc gaagtcgaac agaaactgct
     1201 ggactttatg tcggaatggg tacccggacg cgccacgccg atgtgcggca actccatcca
     1261 ccaagaccgg cgttttatgg tcaaatatat gccgaaactg gaaaactact tccactaccg
     1321 caacctcgac gtttccacgc tgaaagaact cgccaaacgc tggaatccgc ccgttgccaa
     1381 aagcgtcgtc aaacgcggtt cgcacaaggc attggacgac attttggaga gcatcgaaga
     1441 aatgcgccac taccgcgaac actttctgat ttccgccccg agagccgaag cgcaataaga
     1501 aacaaacaat gccgtctgaa acgcagtttg catttcagac ggcattttta cagcagattg
     1561 aaatcaaaaa tatacacgcc cgtcattccc gcacaggcgg gaatccggaa ggtcgggcct
     1621 gccgttattt tcaatcatta cagaaactga aaggtctgga ttcccgcctg cgcgggaatg
     1681 acgggcgtgt gcattcttat agtggattaa caaaaatcag gacaaggcga cgaagccgca
//
LOCUS       DAWGRZ010000231         1723 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.231, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000231 DAWGRZ010000000
VERSION     DAWGRZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.231"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(47..1330)
                     /gene="hemL"
                     /locus_tag="WHT44_001981"
     CDS             complement(47..1330)
                     /gene="hemL"
                     /locus_tag="WHT44_001981"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ2534105.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVETVCEAALGGLSFGAPTEGEIVIAEEIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNEIACVIVEPFVGNMNLVRPTEA
                     FVKALRGLTEKYGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIQREGFYENLTARTEQLVQG
                     FRTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIEGFKQFFHGMLDRNVAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAE"
     gene            complement(1394..1567)
                     /locus_tag="WHT44_001982"
     CDS             complement(1394..1567)
                     /locus_tag="WHT44_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ2534106.1"
                     /translation="MKSERRHWEWCVDYDGEGVVRDYRFTHKVEKDERSVIRDAVGVI
                     RKEAGKSLSQPEK"
BASE COUNT          375 a          498 c          468 g          382 t
ORIGIN      
        1 caatctgcca aacagatcga acagagaaac cctgtccgtc aaaacatcat tcagccatcg
       61 ccttgaacac ttcaaccgca accgcaaccg tttcgtcaat cagctcgggc gtatgcgcgg
      121 cggaaacgaa acctgcttca taagcggacg ggccgaaggc gacattgcgg tcgagcatcc
      181 cgtggaaaaa ctgtttgaag ccttcaatat tggaacgcgc catatcggca tagtttcgcg
      241 gcgcgtgtgc ggcgaaatac agaccgaaca taccgcccac gctgtcggcg gtgaactcga
      301 tgcccgccgc atccgctgcc gtccgaaaac cttgaaccaa ctgttcggta cgcgccgtca
      361 ggttttcata gaagccttcg cgctggatga tttccagcgt tttcaagcct gcggcgacag
      421 caatcgggtt gcccgacaag gtgcctgcct gatacacgcc gcccagcggg gaaatacatt
      481 ccataatgtc tttgcgcccg ccaaacgcgg caagcggcat accgccgccg atgactttgc
      541 ccatcgtggt caggtcgggc gtgatgccgt gcaaagattg cgcgccgccg agcgcgacgc
      601 ggaagccggt catcacttcg tcgtaaatca acaccgcgcc gtatttttcg gtcaatccgc
      661 gcaaggcttt gacaaaggct tcggtcgggc ggacgaggtt catattgccg acgaagggtt
      721 cgacaatcac gcaggcgatt tcattgccgc tttgagcaaa ggcttcttcg agttgggcga
      781 tattgttgta ctcgagtacc aaagtgtgtt tggtaaagtc ggcaggcaca ccggcggaag
      841 acgggttgcc aaacgtcagc agaccgctgc cggctttcac cagcaggctg tcggaatgcc
      901 cgtggtagca gccttcaaac ttgatgattt tgtcacgccc ggtaaaaccg cgtgccagac
      961 ggatggcggt catggtcgct tcggtaccgg agctgacgag gcgcagccgt tcgacggacg
     1021 gcatgatttt ggcgatttct tcggcaatga cgatttcgcc ttcggtaggc gcgccgaacg
     1081 acaaaccgcc caatgcggct tcgcatacgg tttcgacgac ttcggggtgc gcgtgtccga
     1141 caatcgcagg tccccacgag ccgacgtaat cggtatagcg cgtgccgttt tcgtcccaaa
     1201 catacgcgcc ttcggctttt ttgataaagc gcggtacgcc gccgacgctg ccgaatgcgc
     1261 ggacggggga attcacgccg ccggggatga tggctttggc gcggtcgaat aaaatttcgt
     1321 tacggttcat atatatcctc aaatgccgtc tgaacggcag gtttcgggct tggaagcaga
     1381 aagccccatt ttatcatttt tcaggttgcg acaaggattt gcccgcttct ttgcggatca
     1441 cgccaaccgc atcccggatg acggaacgct cgtctttttc cactttatgt gtaaagcggt
     1501 agtctcggac gactccctcc ccgtcgtaat ccacacacca ctcccaatgt cggcgttctg
     1561 atttcatata aatgaaattg gtcggcaaaa aattataaat cggcaggctg acttcatgat
     1621 aggcataaca accgaaaggg ttgcgcttcc cgaaacgtgc ctctacacct ccgcccgggt
     1681 cgttttgcct ttaacaaccg tttgtgcgat tccctcttcc gtc
//
LOCUS       DAWGRZ010000232         1721 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.232, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000232 DAWGRZ010000000
VERSION     DAWGRZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.232"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(62..1276)
                     /locus_tag="WHT44_001983"
     CDS             complement(62..1276)
                     /locus_tag="WHT44_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ2534107.1"
                     /translation="MSEVEYFSHFISDGKGKLLEIPQRRGKQDGVFVDWISFTFHEDT
                     LLKVSGCPLFSDAEYMYVLSRKLEEILGFGITRKCKSRGNKFYESMYRLGSDDVDYGE
                     VHFGGQRNTVLVELKGTGCSVASPGWELRLKQFLDDSIRTRITRIDLALDFFDGEYTP
                     DQALLDHDNGFFDNSNQRPKSETIGTAWRNEDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNYGDIEIPLDILINQGSYFCGAFPICRKFKNMPVPERFDQRKKK
                     LNLTFEHKLHYAKNAVGKLVNFMIEMGFDNSEIVESLKADSGFPKGLEPEKYALEMLR
                     DGLKHGFIHEQPDIDLEIELDELGVIAFKNSDKFDREKRLFSPDYDVEKERKYQEYLS
                     KVYHQNVDYDYF"
BASE COUNT          533 a          409 c          248 g          531 t
ORIGIN      
        1 gctcccaaaa aagttgcagg ataagttaca gtttgagttt gattaaacat tttgatttcc
       61 tttaaaaata atcataatct acattttgat gataaacttt acttaaatat tcctgatatt
      121 tcctttcttt ctcgacatca taatcaggac taaaaagcct tttttcccta tcgaatttgt
      181 cagaattttt aaaagcaata acccccaatt catcaagttc aatttccaaa tcaatatccg
      241 gctgttcatg aataaaaccg tgtttcaaac cgtcccttaa catttccaga gcatattttt
      301 caggttctaa tcctttggga aatcccgaat ctgcctttaa agattccaca atttcgctat
      361 tatcaaaacc catttcaatc atgaaattga ccagttttcc aaccgcgttt ttcgcgtaat
      421 gcaatttatg ctcgaaagtt aaattaagct ttttctttct ctgatcaaac ctttcgggaa
      481 ccggcatatt tttaaatttt ctacaaattg gaaaagctcc acagaaatac gaaccctgat
      541 ttattaaaat atccaagggt atttctatat ctccataatt aaactggatc tcgaacctta
      601 cccatttgct ttctttatct ccaagctgcc tgcctttctc ataaacacga acaaaacgag
      661 aatttttctt gcgacctaca taaaatgtct tgccgctccc gtcctcattc cgccaagccg
      721 taccgatcgt ttcagatttc ggcctttgat tgctgttatc aaaaaaacca ttatcgtgat
      781 ctaacaacgc ctgatccggc gtgtactctc catcaaaaaa atcaagtgct aggtcaattc
      841 gcgttattct tgtccttatc gaatcatcga gaaactgctt tagcctcaac tcccaacccg
      901 gacttgcaac gctgcaacca gtacctttca actcaactaa aacagtattg cgctgacctc
      961 cgaaatgcac ctctccataa tcaacatcat ccgaacctaa cctatacatg gattcataga
     1021 atttgttgcc ccttgatttg catttgcgcg ttatgccaaa acctagaatt tcttccagct
     1081 ttctgcttaa tacatacatg tattcagcat cagaaaataa agggcaaccg gaaactttca
     1141 gtaaagtatc ttcatggaat gtgaatgaaa tccaatcaac aaaaaccccg tcttgcttac
     1201 ctcttcgctg cggaatttct aaaagcttcc cttttccgtc cgatataaag tgtgagaaat
     1261 attcaacttc actcattcgt tcactactct tggaattggt ttttaatttc gctccccccc
     1321 tgttagtcaa ggggggggct ttcagccgtt tcccgtcttc cgcgctaaag cgcgtacgac
     1381 ggtcaacgac cgaaagccaa atcctgacaa acttttaaag atcaacaaaa aagaccacaa
     1441 ccatccgttg tgataatcac tggaaaattc gaaccaaccg aatctatata atcgaacgcc
     1501 tgataaagct ttgaaaaatt ctcttgatta atgagtttat gcggttcgcc atgtctgaac
     1561 tgataaaaac acaaaacaca agaatctgat tttttaaata ttctccaata agaacaggaa
     1621 aatattacat ttactgctga cataaaaaag cccctttcac ttggctgtca aaggggaatg
     1681 ttaagaaaat taatgcgccc ctttgataga gcgcatcata t
//
LOCUS       DAWGRZ010000233         1718 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.233, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000233 DAWGRZ010000000
VERSION     DAWGRZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.233"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            342..1646
                     /locus_tag="WHT44_001984"
     CDS             342..1646
                     /locus_tag="WHT44_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ2534108.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDATAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGVVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          351 a          431 c          509 g          427 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggatcggat gcgatatttc catattcaaa caagggattg gcggcacatc ggcaaaatcc
      241 ccgcgccgcc ccggtccggc agggcttgcg tccctcccgg acaagccccg accccgcctt
      301 tccgaaagac gggctcaacc attaaggaaa ctttagtcaa aatgaaaaaa cacatatggg
      361 cggcatcttt gctgccggca tccctatcgg cagaaccttt aaactggtgg aagccttatt
      421 ccgccgtcaa ttcgggcgat accgcctggg tgatgactgc ggctgccttg gtactgttga
      481 tgacgcttcc cgggctggct ttattctacg gcggtatggt gcggaaaaaa aacctgctct
      541 cgacgatgat gcacagcttt tccatcgcga cattggtggg catcctttgg gtcgccgtcg
      601 gctattcttt agcgttcacg ccgggaaatg cctttatcgg cggtttgggg cgcgtatttt
      661 taagcgggat gcagatagac gctaccgcac agatgctgac cgtgtcgccc aatgcgccga
      721 ctgttcccga accggtattt atgttttttc agatgacgtt tgccattatt tcgaccgcca
      781 ttattaccgg cgcgtttgcc gaacggatga aatattcggc aatgatgctg ttttcgggca
      841 tatggttttt attggtttat gtgccgggcg cgcattgggt gtggggcggc ggctttatga
      901 gcaagggcgg cgtattggat tatgccggcg gtacggtggt gcacatcaat gccggtatcg
      961 cgggactcgt cgccgccttg gttttgggca ggcgcatagg ctacgggcgc gaggcgatgc
     1021 ctccgcacaa tatggcgatg acactgatcg gcgcggcaat gttgtggttc ggctggttcg
     1081 gctttaacgc cggatcggcg cttgcggcag acgcggcggc gggtatggcg atggcggtaa
     1141 cgcaggtgtc ggccgtattc ggcgcggcag gctggcttgc ctgcgaaaaa atagcgggac
     1201 acaaaccttc cgctttgggg ctggcttccg gcgcggtttc cggtctggtc ggcatcaccc
     1261 ctgccgccgg ctttaccggc ccgtcgggcg cggccgccat cggtatattg actgccgccg
     1321 cgtgctttgt gtccgtcacc gtcgtcaaac acaaattgcg ttacgatgat tctttggacg
     1381 ctttcggcat acacggattc ggcgggctgg tgggcggaat attgaccggc atctttttcg
     1441 acaaccgcat tttcggcggg gatgcggcag tttggcagca gttgtggata caggtaaaag
     1501 acggggtcgt tatggcggca tacagcgggc taatgagttg ggcgattttg aaggtcgtgg
     1561 ggaaaatctg cggcggcctg cgcgtcggca aggatgtcga acgcgaaggt ttggatctga
     1621 atatccacgg cgaacgcgtg gaataagggc ggctatgccg tctgaagcct gaaaatcggg
     1681 tttcagacgg catttttcac gtttgccgcc gatggata
//
LOCUS       DAWGRZ010000234         1687 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.234, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000234 DAWGRZ010000000
VERSION     DAWGRZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.234"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     regulatory      74..118
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            123..596
                     /gene="queF"
                     /locus_tag="WHT44_001985"
     CDS             123..596
                     /gene="queF"
                     /locus_tag="WHT44_001985"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ2534109.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFETLARKRLFEHDAQ"
     gene            671..1357
                     /locus_tag="WHT44_001986"
     CDS             671..1357
                     /locus_tag="WHT44_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ2534110.1"
                     /translation="MYAFTAAQQQKALFRLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYAIGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDGFMAANWQGIAFVDYLFKLTVCTLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
BASE COUNT          402 a          510 c          357 g          418 t
ORIGIN      
        1 acggaaaacg ccgccgtcca aaccatgccg tctgaagaaa actacacaga taccgccgct
       61 tatattacaa tcgccgcccg tggttcgaaa acctcccaca ttaaaaaact aaggaaaccc
      121 tcatgtcccg caacaacgaa gagctgcaag gcatctctct tttgggcaat caaaaaaccc
      181 aatatccgac cggctacgcg cctgaaattc tcgaagcatt cgacaacaaa catcccgaca
      241 acgactattt cgtcaaattc gtctgtcccg agttcaccag cctctgcccc atgaccgggc
      301 agcccgactt cgccaccatc tacatccgct acattccgca catcaaaatg gtggaaagca
      361 aatccctgaa actctacctc ttcagcttcc gcaaccacgg cgattttcat gaagactgcg
      421 tcaacatcat catgaaagac ctcattgccc tgatggatcc gaaatacatc gaagtattcg
      481 gcgagttcac accgcgcggc ggcatcgcca ttcatccgtt tgccaactac ggaaaagcag
      541 gcaccgagtt cgaaacattg gcacgcaaac gcttgtttga gcacgacgca caataaacca
      601 tccttcccat atttcagacg gcattccttc gatacgatat gccgtctgaa cgcctttccg
      661 aaagattttt atgtacgcat ttaccgccgc acagcaacag aaggcactct tccggctggt
      721 gctttttcat atcctcatca tcgccgccag caactatctg gtgcagttcc ctttccaaat
      781 tttcggcatc cacaccactt ggggcgcatt ttcctttccc ttcatcttcc ttgccaccga
      841 cctgaccgtc cgcattttcg gttctcactt ggcacggcgg attatctttt gggtgatgtt
      901 ccccgccctt ttgctttcct acgtcttttc cgttttgttc cacaacggca gttggacagg
      961 cttgggcgcg ctgtccgaat tcaacacctt tgtcggacgc atcgccttag ccagctttgc
     1021 cgcctacgcg atcggacaaa tccttgatat ttttgtattc aacaaattac gccgtctgaa
     1081 agcgtggtgg attgcaccga ccgcatcaac cgtcatcggc aacgccttgg atacgctggt
     1141 atttttcgcc gttgccttct acgcaagcag cgatggattt atggcggcaa actggcaggg
     1201 catcgctttt gtcgattacc tgttcaaact taccgtctgc accctcttct tcctgcccgc
     1261 ctacggcgtg atactgaatc tgctgacgaa aaaactgaca accctgcaaa ccaaacaggc
     1321 gcaagaccgc cccgcgccct cgctgcaaaa tccgtaaaac agccctgccg ggaatcggga
     1381 cggttgggtt ttcttttatt tgcaaacact gtcggaacgg ataaatccgg attcccgccc
     1441 acttcgtcat tcccgcgaaa gcgggaatcc ggtacgttgg gtttcagtca tttccgataa
     1501 actcctgccg cgttgtattt ctagattccc gctttcgtgg gaatgacagc ggatgggttt
     1561 ccgtgccgtg gtgtcaggat tcccgcccgt acgggaatga cggcagtgct tccttggctt
     1621 agttttgttt taatcaactg catagtggat taacaaaaat caggacaaga cgacgaagcc
     1681 gcagaca
//
LOCUS       DAWGRZ010000235         1682 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.235, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000235 DAWGRZ010000000
VERSION     DAWGRZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.235"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(195..1364)
                     /locus_tag="WHT44_001987"
     CDS             complement(195..1364)
                     /locus_tag="WHT44_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ2534111.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNYQLQLTEAQ
                     AANGGASGQVKVTKVTKAKSRIRTKISDFGSFIGFKGSEDLGDGLKAVWQLEQDVSVA
                     GGGATQWGNRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRH
                     DDMPVSVRYDSPEFSGFSGSVQFVPIQNSKSAYTPAKDTNNNLTLVPAVVGKPGSDVY
                     YAGLNYKNGGFAGNYAFKYARHANVGRNAFELFLIGSGSDQAKGTDPLKNHQVHRLTG
                     GYEEGGLNLALAAQLDLSENGDKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERG
                     KKGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          433 a          429 c          387 g          433 t
ORIGIN      
        1 tatatgtgca aaccaagcca aaaatgcgaa ataccgtctg aaaatctttc agacggtatt
       61 tgctgtcttt attgccgttt ttcttccgta tccggatttt tgtttggggc tgaagcagat
      121 tggcagtcag attgcaatca aagaatgaag gcgagccgtc aaaaacaaag ctatccgctt
      181 caccgccccg atatttagaa tttgtggcgc aaaccgacgg aggcggcatt aatttgagtg
      241 tagttgccga tgccggtatt gcgtttcagc caagcgccag acacgatggc ggaagtgcgt
      301 ttggaaaaat cataatcaac gccggcgatg atttgatcgt agctggtatt ttcgcctttt
      361 ttaccgcgtt cgataaagtc gaaaccatgg gcatagctga tgcgtggaac tgcattaccg
      421 aagcggtagg aagcagtggc ggcaatttcg gtcgtactgt ttttggtttt gtcgccattt
      481 tcagacaaat ccaactgagc cgccaaggcg agattcaagc cgccttcctc atagccgccc
      541 gtcagacggt gtacctgatg gtttttcaag ggatcggtac ctttggcttg atcactcccg
      601 ctgccgatca agaacaactc aaaagcatta cgtccgacat tggcgtgtct cgcatattta
      661 aaggcatagt tcccggcaaa accgccattt ttgtaattca gaccggcata atacacatcc
      721 gatccgggct tgccgacaac agccggaacg agagtaagat tattgtttgt atccttagcc
      781 ggcgtatagg cggacttgct gttttggatc ggaacgaatt gaacgctgcc gctgaaaccg
      841 gaaaattcgg gggaatcgta gcgtacggaa accggcatgt cgtcgtggcg tttgaaaata
      901 cccaattgcg aagccacatc attattgctg tcccaaggat caatggcttg gctggcatcg
      961 tcaaactgat tcgcaacgcg accggcgcgc agcgtaccga attcgcctgc caagccgata
     1021 aaggattccc tgttgcccca ctgggtcgcg ccgccgccgg caacggatac gtcttgctca
     1081 agctgccaaa cagccttcag cccgtcgccc aaatcctcac tccccttaaa gccgataaac
     1141 gagccgaaat cactgatttt cgtcctgatg cggcttttgg ccttagtaac tttagtaact
     1201 tttacctgac cgctcgctcc accgttagcg gcttgtgctt cagtcaattg cagctggtag
     1261 ttcctgcctt ccacgccggc tttgatttcg ccgtataggc tgacatcggc aacggccgca
     1321 agcggcagtg cggacaatac gagggcggta agtttttttc gcatatcggc ttccttttgt
     1381 aaatttgata aaaacctaaa aacatcgggc aaacacccga tacgtcttca attatacccc
     1441 ccccccccgc aaaaaaccat ttttcagaac aaatatctga taaatgccgc aacctttatt
     1501 ttaaaaatga ttatattttg atataaaaca atagcttatt ttttcaaaaa cgttgtgttt
     1561 ctacaacaca attcaagcgc agacctcgtg cgagccgatg cgctgctgcc cggatgcagt
     1621 ctcggctttt taaaacgcca taaaaaaaca cacgcggcac tttatagtgg attaacaaaa
     1681 ac
//
LOCUS       DAWGRZ010000236         1651 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.236, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000236 DAWGRZ010000000
VERSION     DAWGRZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.236"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(470..1579)
                     /locus_tag="WHT44_001988"
     CDS             complement(470..1579)
                     /locus_tag="WHT44_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ2534112.1"
                     /translation="MSNWKPNIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIVIGRDKLFIHPRLMELLRGEGNSFTSF"
BASE COUNT          378 a          350 c          416 g          507 t
ORIGIN      
        1 gggatatagg tttccatgcg gacgcgttcg gattcacgac tgcgcggaaa tgacgggatt
       61 ttggtgtatt ccctaaaaaa ataaaaaaac atttgcaact ttgttaaaaa taaaggctgt
      121 gttttaacga tgtgttgata tttaatttta gaaaggtagc tatttaatag ttaccttttc
      181 ttatttaaaa atagctttct caaattccat gaacgcctca atacgatatg cagatgctct
      241 atcgaaatta agtttcaaca ttttgtttat taaacatttt attttagcca tttttcaata
      301 tacccccaaa tataccccca atttgcacaa gtcaaaattt taggcagggg ttttgttgct
      361 cccatgatac gcgggcggat gccggctttt cgcgggggaa atacaagggg tctcggttcg
      421 ggtgtcaaaa tccctgtttc gtgttagtca tgtggggggg aagagggggt tagaatgaag
      481 taaagctgtt gccctctccc cgcaatagtt ccattaggcg cggatgaatg aatagtttgt
      541 ccctgccgat gacgatttct tgcagcacac ctatgtctga aagctctttc aggtacttag
      601 aggccgtctg ccgtttggct atccctgccg cttctaggtt ggcaatgcgt gtatatggct
      661 gctcaaacag aagatttacc agttcgtgcg tgtagattcc ttgtgcgtgt gtccgtatgt
      721 gttgccgtgt ctgctcgaac aggcggcgta tcgcatctat tttcgatacc gtccaatcgg
      781 cggtgtcagc tacgccgtct aagatgtaga ttatccagct ttcccagtcc tgccgttcgg
      841 ttacgcctaa aagcaggcgg taatagtccg ccctgttttc gatgatgtag cggctcaaat
      901 acaaaatagg caaatccaaa agcccttttt caatcaatag caggctgttc aatatgcgcc
      961 ccgtccgccc gttgccgtcc gtaaacggat ggatggcttc aaattggtaa tgtgccgccg
     1021 ccatgatgat aagcgggtct aaatcgccgc tttcgtgaat aaaccgctcc caatttgcca
     1081 gcttgccgcg tatggtttct tctccttcgg gcggggtata gacaacattt ccgctgttgc
     1141 ctccttttag ggctgtgccg cctgttttgc ggatggccat ttcgtagggg tgcttgatgg
     1201 cgttgcagac catgatggcg gtttgtgtgc ataaagggcg gctcgtcagt gattcatagc
     1261 ctgcaaacag ggcggtgcgg tattgcaggg cttctttcgt ggcagggtct tgccgttccg
     1321 tatccatttg cagggattga aacagcttgt ccgtggtggt tacgatgttt tcaatttccg
     1381 aacttgcacg ggcttccata acaggaaggg tgttaatcag catggcttga ttcggtatca
     1441 attctgccgc ctgctttaaa cgggcaaggg atgcacgggc ggctatacaa cgtttcagga
     1501 tggttttgct ttcaatatcc tgttttggcg gcaggggtgg taaatcgtta tagggaatat
     1561 tgggtttcca gttgctcata tttaaaattt cggaaaattt aaagatgttt ccagtatatg
     1621 tttacgccgt gtatatatca aggatatatg t
//
LOCUS       DAWGRZ010000237         1647 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.237, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000237 DAWGRZ010000000
VERSION     DAWGRZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.237"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(18..680)
                     /locus_tag="WHT44_001989"
     CDS             complement(18..680)
                     /locus_tag="WHT44_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ2534113.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLIKLGAGITPDHPDYARWRTEYLDEYDSRYAQDTTLFDGVNELIAELGKRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSVKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMTTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(718..1545)
                     /locus_tag="WHT44_001990"
     CDS             complement(718..1545)
                     /locus_tag="WHT44_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ2534114.1"
                     /translation="MKPDIYALLERALLSGDPDEKGRLTDEAFAAVQNADGAETNAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTTEGYAAMLHAIAHIEFNAINLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRERLRAFGFDYGDFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNRWYQHLCRE
                     RGLEPVALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKHAV"
BASE COUNT          317 a          436 c          506 g          388 t
ORIGIN      
        1 tgtggggcgg atatttttca gggctgtatt ttgtccagac attcgagcag atcgagtggc
       61 gtgcggatgt ggaaatccgc ctgccatgag ccggtatcgt cttcgggagc gatgtagccc
      121 cattcggcga ggacggtcgt cataccggcg ttgcgccccg cctgtatatc gcgttccgcg
      181 tcgccgacgt agagtgtgtg ttgcgggtcg gcgtggattt gtccgcacgc atacagcatg
      241 ggtttgacgc tgggcttggg ctcgccgcag gtgtcgccgc tgacgacgac ggcgggtggg
      301 atgatgaagc cgagtttggg gacgagtttg tcggtgaagc gcatgggttt gttggtgatg
      361 atgccccatt tgatgccgcg ttttccgagt tcggcgatga gttcgtttac gccgtcgaag
      421 agggtggtgt cttgggcgta gcggctgtcg tattcgtcaa ggtattcggt gcgccatcgg
      481 gcatagtcgg gatggtcggg ggtgatgcct gcgccgagct tgatcagtcc tgccgcgccg
      541 tggctggctt gggtgcggat ttcgtccatg ctttttgcag gtagtccgtg gcgggcgagc
      601 agggtgttga gtgcgccgcc gaggtctagg gcggtgtcgg cgagcgtgcc atcgaggtcg
      661 aacaatacgg cttgtatcat gtgtgttcct tttttataaa gtgcgggacg aagggtttca
      721 gacggcatgt ttattttgtt tcaaaccctg ctcgaaatct tccaacatat ccaattcaaa
      781 gcggctgaag cctgcttttt cgcgcgcttc gatgttcaca tagccccgga agataaacat
      841 atcgtaacgg gcaatcaggc tgcggaacag ggcgacaggc tccaaaccgc gttcgcggca
      901 aaggtgttga taccaccggt tgccgatggc gacgtgtccc acttcgtcgc ggtaaatgat
      961 gtccaacacg ccgcaggttt ccgaatcacc gcgctgcgcc accttcgcgc gtatgccggg
     1021 cgtaacgtcc agcccgcgcg cttccaaaac gcgcggcact aaagccatac gcaacaaagg
     1081 atcgtaggcg gttttgtatg ccatatccca taaatgattg tgtgcttcaa aatcgccgta
     1141 atcgaagccg aaagcgcgca gcctttcgcg catcaggcgg aaatggtaca cctcttcctt
     1201 cgccactttc acccagtcgc ggacaaactg aaacggcagc gtgcggaaac ggtatgccgc
     1261 gtccaaagcc agattgatgg cgttgaattc gatatgcgca atcgcgtgca gcatcgccgc
     1321 atagccttcg gttgtgttca ttttgcgtgg cgtcagctgc gacggcgcga ccaaaacagg
     1381 cttgtccggt cgtcccgcgc gggggaagtc cgccggcggt gcgtttgttt ccgccccgtc
     1441 cgcattttga acggcggcaa acgcctcatc cgtcagccgt cctttttcat ctgggtcgcc
     1501 cgaaagcagg gcgcgttcca gcaaagcata aatatcgggt ttcatctcaa gtccgccgtg
     1561 ttcggaaaac aaatattata gcgtttaaaa aaaacaagat gaggcatata atctccgcga
     1621 ttcggcattc cgcgcccaaa ccgtcaa
//
LOCUS       DAWGRZ010000238         1628 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.238, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000238 DAWGRZ010000000
VERSION     DAWGRZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.238"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            66..1508
                     /gene="aldA"
                     /locus_tag="WHT44_001991"
     CDS             66..1508
                     /gene="aldA"
                     /locus_tag="WHT44_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ2534115.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEEAIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRERADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
BASE COUNT          404 a          464 c          433 g          327 t
ORIGIN      
        1 ttgtacggca atttatttgg gaataaacta aaactacatc taactacaaa actggagaac
       61 ccgaaatgaa acaattggcc atgtacatca acggacgctt tgaaaacgat ttcaacggcg
      121 aatggcgcga cgtattgaac ccgtccaccg aagaggccat cgcccgcgaa cccaaaggcg
      181 gcaaggcgga cgttgaccgc gccgtcgcgg cggcgcgtgc ggcgcaaccg gcttgggagc
      241 gtctgcctgc ggtcgaacgc ggcgcgtatt tgcgtaaaat cgcccaaggc atacgcgaac
      301 gtgccgacga gctgaccgac accatcgttg ccgaaggcgg caaaaccaaa gacttggcac
      361 gcgtggaagt catgttcacc gccgactatc tcgattatca ggccgaatgg gcgcgccgct
      421 acgaaggcga aatcatccaa agcgaccgcc cgcgcgaaaa tattttattg ttcaaacgtc
      481 cgctgggcgt aattgccggc attttgccgt ggaacttccc cttcttcctg attgcccgca
      541 aaatgggccc cgctttggta acgggcaaca ccatcgtcgt caaacccagc agcgtaaccc
      601 cgatcaactg ccacatcttc gccgaaatcg tcgatgcggt cggactgccc gcaggcgtgt
      661 tcaacgtggt gaacggtccc ggcgcggaaa tcggcaatgc cttgtccgcc catccgcaag
      721 tcgatatggt cagcctgacc ggctccgtcg aagcaggccg ccaagtgatg gaagccgcct
      781 ccgccaacat caccaaagtt tcgctggaac tcggcggcaa agcgcctgcc atcgttttga
      841 aagatgcgga tttggacttg gcggtgaaat ccatcttggc ttcgcgcgtc ggcaacaccg
      901 gtcaaatctg caactgcgcc gagcgcgtct atgtccacag cagtctgaaa gacgcattca
      961 ttgaaaaaat gaccgccgcg atgaaaggcg tgcgctacgg caaccctgcc gaagccgaag
     1021 caggcgcgct ggaaatgggc ccgctgattg aagaacgcgc cgtcaaagcc gttgccgaaa
     1081 aagtggaacg ggcagtcaaa caaggtgcga aattggtttg cggcggcaaa cgcgccgaag
     1141 gacgcggtta tttcttcgag ccgaccctgc tgaccgacac cgacaacagt atggacatta
     1201 tgaaagaaga aaccttcggc cccgtgctgc ccgtttccgc tttcgacacg ctcgaccaag
     1261 tcatcgcctt ggcaaacgat tgcgagtttg gtctgaccag ttctgtttat acgactaatt
     1321 taaacgaagc cttctacgtt acccgccgcc tgcaattcgg cgaaacctac atcaaccgcg
     1381 aaaactttga agcgatgcag ggtttccacg ccggttggaa aaaatccggt atcggcggcg
     1441 cggacggcaa acacggtttg gaagaatatc tgcaaaccca agtcgtttat ttggaaaccg
     1501 acatttaatg ccgctttaaa accccgatag aaaatgccgt ctgaacccgt tttcaggttc
     1561 agacggcatt tttattgctt caccggcaat cagtcatgac cgaggtcgat gtttttgtct
     1621 ttgtatag
//
LOCUS       DAWGRZ010000239         1595 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.239, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000239 DAWGRZ010000000
VERSION     DAWGRZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.239"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            62..1435
                     /locus_tag="WHT44_001992"
     CDS             62..1435
                     /locus_tag="WHT44_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ2534116.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNIHLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGNLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKPEMREPRQRNRYESAKAQREKNTRPENAANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
BASE COUNT          412 a          525 c          390 g          268 t
ORIGIN      
        1 ttccccgtcc tatcggtttc ccgtttcaga cgacataagg tctgaaagaa agactacaat
       61 tatgagtaat ccattttcct ctttaggttt gggtacggaa ctcgtttccg cactgaccgc
      121 gcaaggttac gaaaacccga cgcccatcca agccgccgcc attcccaaag cactcgccgg
      181 tcatgatttg ctagccgccg cgcaaaccgg cacaggcaaa accgccgcct ttatgctgcc
      241 cagtctggaa cgcctcaaac gttacgccac cgccagcacc tcgcccgcga tgcaccccgt
      301 gcgtatgctc gtcctcaccc ccacgcgcga acttgccgac caaatcgacc aaaacgtgca
      361 gggctacatc aaaaacctgc cgctgcgcca caccgtcttg ttcggcggta tgaatatgga
      421 caaacagacc gccgacctgc gtgccggctg cgaaatcgtc gtcgccaccg tcggacggct
      481 gctcgaccac gtgaaacaga aaaacatcca tttgaacaaa gtcgaaatcg tcgttttgga
      541 cgaagccgac cgtatgctgg atatgggttt tatcgacgac atccgcaaaa tcatgcagat
      601 gctgccccgc caacgccaaa ccctgctctt ttccgccacc ttctccgccc cgatacgcaa
      661 actggcgcaa gacttcatga acgcgcccga aaccgtcgaa gtcgccgcgc aaaacaccac
      721 caacgccaac gtcgagcagc acatcatcgc cgtcgatacc attcagaagc gcaacctgct
      781 cgaacggctg attgtcgatt tgcatatgaa ccaggtcatc gtgttctgca aaaccaaaca
      841 aagcgtcgac cgcgtaacgc gcgaactggt gcgccgcaac ctgtccgcac aggcgataca
      901 cggcgaccgt tcccaacaaa gccggctcga aacactcaac gccttcaaag acggcaacct
      961 gcgcgtcctc gtcgccaccg acatcgccgc gcgcgggctg gacattgccg aactgccctt
     1021 cgtcatcaat tacgaaatgc ccgcccagcc cgaagactac gtccaccgca tcgggcgcac
     1081 ggggcgcgcg ggcgcggacg gcgtggcgat ttccctgatg gacgaatccg aacagaaaat
     1141 gtttgaatcc attaaagagc tgaccggcaa caagctgctc atcgagcgca tcgagggctt
     1201 cgagccgcaa tggtgggaac agggcggcgc aaaaccggaa aaacccgaaa tgcgcgaacc
     1261 gagacaacgc aaccgctacg aatccgccaa agcgcaacgc gaaaaaaaca cccggccgga
     1321 aaatgcggca aacgatgcgg gcgcggcttg cggaaaaatt gccggacgca gccgccgaag
     1381 ccgccgggaa caccggacgt gcgccctgct ccaaccgcgt tacggcgtaa aatagccctg
     1441 aaaatcaaat gccgtctgaa catttcccgt ttcagacggc atttttcaaa ccggactgac
     1501 gcatcgggag caaccgcccg caccggataa atttctgccg caaacagttt cagacggcat
     1561 ttgccgcctg tacaatatag tggattaaca aaaat
//
LOCUS       DAWGRZ010000240         1587 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.240, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000240 DAWGRZ010000000
VERSION     DAWGRZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.240"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..311)
                     /locus_tag="WHT44_001993"
     CDS             complement(<1..311)
                     /locus_tag="WHT44_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
                     /protein_id="HEZ2534117.1"
                     /translation="MTFNQEQDYWAGYKANERALIIQTWSGFGRYAPDHLYPPHILPL
                     DTDNETLGTTVLQALANSRTFVYDSPEDQDFFDTEKIRQRYEDWVAKLCGNLGYKTRR
                     AL"
     gene            complement(312..1193)
                     /locus_tag="WHT44_001994"
     CDS             complement(312..1193)
                     /locus_tag="WHT44_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534118.1"
                     /translation="MRTDAFKYGTWNSLNDIRSSYDRAATKIKGAGNMGLGATTFVGS
                     GAIGGGLCSTGIGCAAGGLIATAGMTGGYTQASEGSRQLFGTYQSDFGKKVVLSLGTP
                     IEYESPLVSDAKNLAVWGLETLITRKLGNLATGVKTSLTPKTADVQRNILSQSEVGIK
                     WGKGIEGQGMPWEDYVGKGLSANARLPKNFKTFDYFDRGTGTAISAKTLDTQTTARLS
                     KPEQLYSTMKGYIDKTANFKSYELSEVPLRADMIKQREIHLAIPAQTNKEQRLQLQRV
                     VEYGKSQNITVKITEIE"
     gene            complement(1365..1587)
                     /locus_tag="WHT44_001995"
                     /pseudo
     CDS             complement(1365..1587)
                     /locus_tag="WHT44_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581202.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin/hemolysin family protein"
BASE COUNT          343 a          412 c          348 g          484 t
ORIGIN      
        1 agggcgcgtc tggttttata gcccaagttc ccgcatagct tggcaaccca atcctcatag
       61 cgttgccgaa ttttttcggt atcaaaaaaa tcttggtctt ctggactgtc ataaacgaaa
      121 gtcctgctgt ttgccaatgc ttgcaagacc gttgtgccta aagtttcatt gtcggtatcc
      181 aatggcagga tatggggggg atataggtgg tctggagcat atcgcccaaa tcctgaccat
      241 gtttgaataa tcaaggctct ttcatttgcc ttatagccag cccaataatc ttgttcttga
      301 ttgaaagtca tttattcgat ctccgtaatt ttgactgtaa tgttttgact tttgccatac
      361 tctaccacac gttgcaactg caatctttgc tccttattag tttgtgcggg tatggccaga
      421 tggatttcgc gctgtttgat catgtctgcc cttaacggta cttctgataa ttcataactt
      481 ttgaaatttg ccgtcttatc gatgtaccct ttcatggtac tgtaaagctg ttcgggtttg
      541 gacaggcgtg ccgtagtttg cgtatccaga gttttggcac tgattgccgt gcctgtacca
      601 cgatcaaaat aatcaaatgt tttaaaattt ttaggtaacc ttgcattggc agacaagccc
      661 ttaccgacat aatcctccca aggcattccc tgtccttcaa tccccttgcc ccacttgata
      721 ccgacttcgg attgggacag gatatttcgc tgtacgtcag cagttttcgg agtcaaggaa
      781 gttttcacac ccgttgccaa gtttcccaat ttgcgcgtaa tcagcgtttc caatccccat
      841 acggctagat ttttcgcatc agatactaac ggcgattcgt attctattgg tgtacccaaa
      901 gataggacaa cttttttacc aaaatcggac tggtaagtgc caaacaattg ccggcttcct
      961 tctgaggcct gtgtataacc accggtcata cctgccgttg caataagtcc accggccgca
     1021 cagccaatcc cggtactgca cagacctccg cctatagcac ccgaaccgac aaaagtcgtt
     1081 gcacccaatc ccatattgcc cgcaccctta attttggtgg cagcacggtc gtaactgctg
     1141 cgtatatcat tcaggctgtt ccatgttcca tatttaaatg catccgtccg catcaatacg
     1201 ttttgttccg ctacaaactg tttaccggca tcttggaggt tttttagtcc tttataaaga
     1261 gggtcgaagt caggtacgcc ttccgcgcac cgggttaatg cacatgcagc ggcttttagg
     1321 cggtactgtt cttcagccga tttgcctttt gacagtttgt taaggatttg tgtttctttc
     1381 ggatgcagct gcctattgtt ccaatcgaca ttcgccccta ctgccgccgt accgacattc
     1441 ccgcccgccg catagccgat tgccgccccg ccccgtgcag tatgctcctg cctatgccgc
     1501 cttctttcca ggtgtcgtag cggctttggt tttcggcaag ataggcgttt acttggccga
     1561 gggatgcgcg gaaggcggct ttttcgg
//
LOCUS       DAWGRZ010000241         1552 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.241, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000241 DAWGRZ010000000
VERSION     DAWGRZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.241"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..190
                     /locus_tag="WHT44_001996"
     CDS             <1..190
                     /locus_tag="WHT44_001996"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534119.1"
                     /translation="GKSQTEKSQTEKSQTENSETENSETENSETENRKKPNRKQEKAK
                     QKKACLATGFLLIPIFAD"
     gene            complement(187..1182)
                     /gene="porB"
                     /locus_tag="WHT44_001997"
     CDS             complement(187..1182)
                     /gene="porB"
                     /locus_tag="WHT44_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ2534120.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVFHQNGQV
                     TEVTTATGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHHQVQEGLNIEKYQIHRLVS
                     GYDNDALYASVAVQQQDAKLTDASNSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGLV
                     DDADIGNEYDQVVVGAEYDFSKRTSALVSAGWLQEGKGENKFVATAGGVGLRHKF"
BASE COUNT          411 a          346 c          396 g          399 t
ORIGIN      
        1 aggaaaaagc caaacagaaa aaagccaaac agaaaaaagc caaacagaaa acagcgaaac
       61 agaaaacagc gaaacagaaa acagcgaaac agaaaacagg aaaaagccaa acagaaaaca
      121 ggaaaaagcc aaacagaaaa aagcctgtct ggcgacaggc tttttgttga taccaatctt
      181 tgcagattag aatttgtggc gcagaccgac accgccggca gtcgctacga atttgttttc
      241 gcctttgcct tcttgcaacc aaccggcaga aaccaaggca gaagtgcgtt tggagaagtc
      301 gtattccgca ccgacaacca cttggtcgta ttcgttgcct atgtctgcat catcaaccaa
      361 acctttgaag ccgtgggcgt aagaaactcg gggcgttacg ttgccgaagc ggtatgccaa
      421 ggtagcggca acttcggttt gagagttgtg cgaattggaa gcatcagtca gtttcgcgtc
      481 ttgttgctgt acggctacgg aagcgtacag ggcatcattg tcgtaaccgc tgaccaaacg
      541 gtgaatctgg tatttctcaa tattcaagcc ctcttgcact tgatgatgtc ttttataggc
      601 accgccatat tgcacgaaga agccaccgtt tttgtagttg aagccggcgt ggtaagattc
      661 gctgttatgt ctgcctgcat tgtcgttaag cgcgtattgt acgctgccgc tgaggccggc
      721 aaattcggga gaatcgtagc gtacggaaat gaggcgtgcc tcgggttcgg caattttgtt
      781 tacacccaaa tagtcgcttt tgctatccca aggattgatg tcgccggtgt ctttcaggac
      841 gctgttcaaa cgaccgacgc gcaatttacc gaagccgcct ttcaagccga tgaaggattg
      901 gcggttgccc caaccggagt cagtaccggc gatagatgct ttttgctcaa cctgccaaat
      961 ggctttcagg ccgttaccga ggtcttcttg gcctttgaag ccgattttcg aacccaaatc
     1021 aacgatgccg gtagcggttg taacttcagt aacttggccg ttctggtgaa atacagagcg
     1081 ggaagtttct acgccggctt tgatggtgcc gtacagggta acgtcagcca ttgctgcaac
     1141 aggaagggct gccaaagtca gggcaatcag ggattttttc attgctgtat tccttttttg
     1201 gttaagaaat ttaagccggc cgggctttcc aagccgctta gctttgcatt taccgccgac
     1261 gttttcggcg attccatgtc tgaaactata gatgttcctg tctgaaaaaa caaacttctg
     1321 cctgtttttg tgtggaattc gatgtgtatt ttgaagggcg gatagagata ttatgggtat
     1381 tttttgtttt ataacatatg gttatttaaa ttttttaaga tttgcatttt tacaacactt
     1441 actcgggagg gtattggagg gcattgcaaa ccgggggtta taaagacggc aaaaaacgcg
     1501 cacgtggttt ctttcggaac gggcggatgc aaaacccgcc cctgcggcag ga
//
LOCUS       DAWGRZ010000242         1540 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.242, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000242 DAWGRZ010000000
VERSION     DAWGRZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.242"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            33..1433
                     /locus_tag="WHT44_001998"
     CDS             33..1433
                     /locus_tag="WHT44_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ2534121.1"
                     /translation="MTPSALKKTVLLLGTAFAAASVHASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNDRHSFGAGIIAQHTSAELRKYADWGIKSKAEILTAKPPKPNGVAEAAKIQAD
                     GHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADGAAAKAMWS
                     TMLAANGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFE
                     KEKTVVKGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMN
                     SLPDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARF
                     KNHADIIGLQYTYKFK"
BASE COUNT          410 a          490 c          367 g          273 t
ORIGIN      
        1 tatccaccgt ccgccttttt acggaaaccg aaatgacccc ttccgcactg aaaaaaaccg
       61 tcctgctgct cggcactgcc tttgccgccg catccgtcca cgcatccggc taccacttcg
      121 gcacacagtc ggtcaacgcg caaagcacgg caaatgccgc cgccgcagaa gccgccgacg
      181 catcgaccat cttctacaac cctgccggcc tgaccaaact cgacagcagc cagatttccg
      241 tcaacgccaa catcgtgctg cccagcattc attatgaggc ggattccgcc accgacttta
      301 ccgggcttcc cgtccaaggt tcgaaaagcg gcaaaatcac caaaaccacg gtcgcgcccc
      361 acatctacgg cgcatacaaa gtcaacgaca atctgaccgt gggcttgggc gtgtacgtcc
      421 ccttcggctc tgccaccgaa tacgaaaaag attccgtgtt gcgccacaac atcaacaaac
      481 tcggtctgac cagcatcgcc gtcgaacctg tcgccgcgtg gaaactcaac gaccgccatt
      541 ccttcggcgc aggcatcatc gcccaacata cttccgccga actgcgcaaa tatgccgact
      601 gggggattaa gagtaaagca gagatattga cggcaaaacc gcccaaacct aacggtgtag
      661 ccgaagctgc aaaaattcag gccgacggac acgccgatgt caaaggcagc gattggggct
      721 tcggctacca actggcgtgg atgtgggaca tcaacgaccg tgcgcgcgtg ggcgtgaact
      781 accgttccaa agtctcgcac acgctcaaag gcgatgccga atgggcggca gacggcgcgg
      841 cggcgaaagc aatgtggagt acgatgcttg cagcaaacgg ctacacggcg aatgaaaaag
      901 cccgcgttaa aatcgttacg cctgagtctt tgtccgtaca cggtatgtac aaagtgtccg
      961 ataaagccga cctgttcggc gacgtaactt ggacgcgcca cagccgcttc gataaggcgg
     1021 aactggtttt tgaaaaagaa aaaaccgtcg tcaaaggcaa atccgaccgc accaccatca
     1081 cccccaactg gcgcaacacc tacaaagtcg gcttcggcgg ttcttatcaa atcagcgaac
     1141 cgctgcaact gcgcgccggc atcgcttttg acaaatcgcc cgtccgcaac gccgactacc
     1201 gcatgaacag cctacccgac ggcaaccgca tctggttctc cgccggtatg aaataccata
     1261 tcggtaaaaa ccacgtcgtc gatgccgcct acacccacat ccacatcaac gacaccagct
     1321 accgcacggc gaaggcaagc ggcaacgatg tggacagcaa aggcgcgtct tccgcacgtt
     1381 tcaaaaacca cgccgacatc atcggtctgc aatacaccta caaattcaaa taaacggccc
     1441 gccgtttgaa tgtaacaatg ccgtctgaaa cagatttccg tttcagacgg cattgtttta
     1501 ggctcgattt atagtggatt aacaaaaatc aggacaaggc
//
LOCUS       DAWGRZ010000243         1507 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.243, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000243 DAWGRZ010000000
VERSION     DAWGRZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.243"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            1..213
                     /locus_tag="WHT44_001999"
                     /pseudo
     CDS             1..213
                     /locus_tag="WHT44_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238292.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            337..1032
                     /locus_tag="WHT44_002000"
     CDS             337..1032
                     /locus_tag="WHT44_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HEZ2534122.1"
                     /translation="MGDGCRNGINMSIKYIAMPAAWKYMPTDVSRVAKEVFGYLYNSQ
                     GASTRFDKYFNTIDNRADFFAASNLYEQNLGSKARWFGGADFVSRAAITGLGADGEAS
                     YITFAAGKVVGNPPIYEWRAASGNALIVNGFYNFRDLFNKKTNPREWDIQQLKSEQKL
                     LQPIHQKYLSNEKDYLSLIKGVTSNKIFSIIPNPLDERKKIEDGINMLDYKSRIKYGC
                     KLMGYSEKQGCKP"
     gene            1020..1463
                     /locus_tag="WHT44_002001"
     CDS             1020..1463
                     /locus_tag="WHT44_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534123.1"
                     /translation="MQAMNKNYLFYLIQNIKSIFLIGGVFFGLLIFSGLWGNDFKLSF
                     LGVIDFIFGMVGGGFIVMLWFLIPVILSTLFRWVFQNQLAVSVILTFIVFYTYGYFSL
                     FNSSERVGSFWYLVIPTIMFIGLSEWFIYKSSVHSLGENVTPRSN"
BASE COUNT          470 a          211 c          344 g          482 t
ORIGIN      
        1 gcggcggtcg gtgaaatcct cggcgaagcc ctgctggacg gcagagaccc gggcagcctg
       61 aatgtgaaag accgggcaaa aatcattgct aaggcgaagc tggcagcagg gacggttgcg
      121 gcgttgagta agggggatgt gaatgctgcg gcgaatgcgg ctgctgtggc ggtagagagt
      181 aatgcgctta gcaaggaaag aatggataaa ttgacaaaat gcctttccgg taaaacttgt
      241 tctactacga tggaaaaagt aaatgccatc aaaaaggatg aacaatttag caaagtaatt
      301 gatacggaaa ttcaaaaagt ctgttctagg aacccattgg gcgatggttg cagaaacggc
      361 attaatatgt ctattaaata tattgccatg cctgctgcgt ggaagtatat gcctacggat
      421 gtatcacggg ttgccaaaga agtttttggc tatttatata actcacaagg ggcatctaca
      481 agatttgaca agtatttcaa caccattgac aatcgtgcag atttctttgc tgccagcaat
      541 ctttatgagc aaaatttggg ttcaaaagca cgatggtttg gtggagctga ttttgtatcg
      601 cgtgctgcta taactgggtt aggggcagac ggagaagctt cttatataac ttttgcggca
      661 ggtaaagttg ttggtaatcc tccaatttat gaatggaggg ctgcgtcagg caatgcactg
      721 atagtaaatg gattttataa ttttagagac ttgttcaata aaaaaactaa tcctagggag
      781 tgggatattc aacagttgaa aagcgagcaa aagttattac agcctattca ccaaaaatat
      841 ttgagtaatg agaaggatta tttgtcattg attaaagggg taacatcaaa taagatattt
      901 tcaataattc caaatccttt agatgagagg aaaaaaatag aggatggaat taatatgtta
      961 gattacaaat ctagaattaa atatggttgt aagcttatgg gatattctga gaaacaggga
     1021 tgcaagccat gaataaaaat tatttatttt atcttattca aaatattaaa tctattttct
     1081 taataggtgg agtatttttt ggccttttaa ttttttcagg tttatggggt aatgatttta
     1141 aactatcttt tttaggggta atagatttta tatttgggat ggtagggggt gggtttatag
     1201 ttatgttatg gtttctaatt ccagtgatat tatcaacttt attcagatgg gtatttcaaa
     1261 atcaacttgc tgtatctgtc atattaacct tcattgtttt ttatacatat gggtactttt
     1321 ctttatttaa ttcatctgaa agagtcggta gtttttggta tttggtaatc ccaaccataa
     1381 tgtttattgg actgtctgaa tggtttatct ataaatctag cgtgcatagt ttaggtgaaa
     1441 atgtaactcc acgttccaat tgaagaaaaa gattgtctga aaactaaatt taatttcaga
     1501 tgacctt
//
LOCUS       DAWGRZ010000244         1494 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.244, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000244 DAWGRZ010000000
VERSION     DAWGRZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.244"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(161..532)
                     /locus_tag="WHT44_002002"
     CDS             complement(161..532)
                     /locus_tag="WHT44_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534124.1"
                     /translation="MRFYIDNKECDYIFFRKAVNISDDFIQSGFITPEGLITKNSNPK
                     LFNQYSKMASKNKCQYEMVTFLSDEMKNIIELLSNDDAYIEFAYSEDFYVLPEIEIDK
                     RTLKSLNFYIDFGVKYIINKI"
     gene            complement(664..1107)
                     /locus_tag="WHT44_002003"
     CDS             complement(664..1107)
                     /locus_tag="WHT44_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534125.1"
                     /translation="MKKQITEATMMLSIIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGAAAPLVAIGILHAGRFLAQRWVTQRVAAQALSRGANVYARTSQQAR
                     AVANQAWGRQNVIRHGQKEIHSNYSHFQNSSQKIRGHAFYGNKHR"
     gene            complement(1195..1419)
                     /locus_tag="WHT44_002004"
     CDS             complement(1195..1419)
                     /locus_tag="WHT44_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534126.1"
                     /translation="MMKNKYILIKAAISITAISIFYLLTQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAVTAMIAAHFIFVALSD"
BASE COUNT          480 a          274 c          223 g          517 t
ORIGIN      
        1 atccactata tatatccatt catatagaga aaaattctta tacccaatat aaaacaaggt
       61 ctttcattat attttgaaaa acctagaagc tctgtatcta ttggtttatc aaaattaata
      121 attattgcag aagacatgta ggctaataat cttgatttat ctatatttta ttaattatat
      181 atttaacccc gaagtcaata tagaaattta gtgattttaa agttctctta tcaatttcaa
      241 tctcagggag tacataaaaa tcctcagaat aggcaaactc tatatatgca tcatcattcg
      301 ataataattc aataatattt ttcatttcat ccgaaagaaa tgtaaccatc tcatattgac
      361 atttattttt agatgccatt tttgaatatt gattaaataa ttttggatta ctatttttag
      421 taattaatcc ttccggagta ataaaaccac tttgtataaa gtcatcactt atattaacag
      481 ccttccgaaa aaaaatataa tcacactctt tgttatcaat ataaaatctc atactcttta
      541 ataatgaaat ataagaattt ccagaattac aagcaatttg aaaaaacatc attactccta
      601 aaactcataa ataataagga atatatatat gcatattcct tactattttt tcacaaatta
      661 tgcttatcta tgcttatttc cataaaaagc atgccctcga attttttgac ttgagttctg
      721 gaaatgagaa taatttgaat ggatttcctt ctgcccatgt ctaataacat tttgtcttcc
      781 ccaagcttgg tttgctaccg ctcttgcttg ttgagatgtt cgtgcataga cattagctcc
      841 tcttgataaa gcttgtgctg caacacgctg cgtaacccaa cgctgcgcaa ggaatctgcc
      901 cgcatgcaaa attcctattg ccaccaacgg tgccgcagcc ccttcagttt ctttcatctc
      961 cttttgagaa agctccgcca actgcatcgg cgcatctgcc cgcgtgtgga acacttggtc
     1021 ttcaaatgcc tgattgtcca atccgtttgc cattgcgggg gcgattatag acagcatcat
     1081 cgtggcttca gtgatttgtt ttttcataac aaattcttag ggaggagata atgttccatc
     1141 aaaatcctcc tgaaaggctg cctgaaattt ccaagcagcc tttttgattg cttattaatc
     1201 ggatagtgcg acaaatataa agtgggcggc aatcattgcc gtaacggcag ccataactgt
     1261 tttattttct tcaaaccaaa gcaagttgag aaacataaac atcaagaaat atgatggttc
     1321 ttcagttttg ccgctgcttt cttgagttag aagataaaat atagatatgg cggttatgct
     1381 gattgctgct tttattaaaa tatatttgtt tttcatcatc ttgtatggtt tttcatgcaa
     1441 gtttcaacaa taaaattcgg aataagatag agagatgttg atatatcaga taaa
//
LOCUS       DAWGRZ010000245         1486 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.245, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000245 DAWGRZ010000000
VERSION     DAWGRZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.245"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            39..462
                     /locus_tag="WHT44_002005"
                     /pseudo
     CDS             39..462
                     /locus_tag="WHT44_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            568..1131
                     /gene="pgsA"
                     /locus_tag="WHT44_002006"
     CDS             568..1131
                     /gene="pgsA"
                     /locus_tag="WHT44_002006"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ2534127.1"
                     /translation="MPWNLPIFLTWLRVLLIPVLTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNFPDFYGFNLAFIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1211..1286
                     /locus_tag="WHT44_002007"
     tRNA            1211..1286
                     /locus_tag="WHT44_002007"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1244..1246,aa:Gly,seq:gcc)
     gene            1312..1387
                     /locus_tag="WHT44_002008"
     tRNA            1312..1387
                     /locus_tag="WHT44_002008"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1345..1347,aa:Gly,seq:gcc)
     gene            1411..1486
                     /locus_tag="WHT44_002009"
     tRNA            1411..1486
                     /locus_tag="WHT44_002009"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1444..1446,aa:Gly,seq:gcc)
BASE COUNT          351 a          360 c          371 g          404 t
ORIGIN      
        1 atcgctgggt atggtttgcc gccataagct gtctgctgtt gtccgccgtt tttattgcac
       61 cttacctgac ggcatttcac gaacaagaaa aaacattcga atatgcggaa ttgaccgtta
      121 ccgccctcaa ccgcagcgga cgggcaatca aactggatgc agacgggcag cattaccggc
      181 tttcctgcta tgggttcgac agtttgtgca caggcggcaa tatcggcaga accatcaggg
      241 cggagcaggt tagagccgtt ttaagcgaaa ccgtcggcaa aggtttttta aacggcgttc
      301 tgttggaata ccgcaacagt ggcagtgtct ataagcaata aagattttgc ccgcacggaa
      361 gaccgccttg tcgaagtgtt ggcacaacct gccgttttca gcctgaaact gggtattttg
      421 cttttattgc ccgccatttt cctgcgtttg aaaagaatgt gaaaacagat tggcggggcg
      481 ggggaatgcc catgcagcaa ttttacgttt tgggttatga ggataaccgt tataatcaca
      541 actttacagt ctgcacagag aaaaccgatg ccttggaatc tccccatctt cctcacatgg
      601 ttgagggtct tgctcattcc tgtcctgaca gccctttttt acctgccttt tccgtggttc
      661 gcggaggaaa cgattaatct caccgccgcc gtcatttttg ccgttgccgc cttgaccgac
      721 tggtttgacg gatttttggc aaggttgtgg aaacagacct cggatttcgg cgccttcctc
      781 gatcccgttg ccgacaagct gatggtcgcc gtctctttgc ttttactggt caaactcgac
      841 cggacctatg ttttgtttgc catgatcatc atcggcaggg aaattaccat ttccgcattg
      901 cgcgaatgga tggcgcaaat gggcaaaagg aacagcgttg ccgtcgccac cgtcggtaag
      961 tttaaaaccg ccgcgcaaat gctggcaatc ttcctgctgc tgctgaattt tcccgatttt
     1021 tatggattta acctcgcatt catcggcaat gtattgatgt ttatcgcatc tttgctgaca
     1081 gtctggtcga tgctgtatta tctgaaaatg gcgtggaaag aaatcgcctg aaaaaaacat
     1141 aaaaatagct tgacggcaaa aacagaatcc ataataattg tgtctcttcg atatcggaga
     1201 atgaaatcgg gcgggaatag ctcagttggt agagcgcaac cttgccaagg ttgaggtcgc
     1261 gagttcgaga ctcgtttccc gctccaaaaa tttcattctt ccgcaatcat tgcgggaata
     1321 gctcagttgg tagagcgcaa ccttgccaag gttgaggtcg cgagttcgag actcgtttcc
     1381 cgctccaagt tttatttcaa acatatcaac gcgggaatag ctcagttggt agagcgcaac
     1441 cttgccaagg ttgaggtcgc gagttcgaga ctcgtttccc gctcca
//
LOCUS       DAWGRZ010000246         1486 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.246, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000246 DAWGRZ010000000
VERSION     DAWGRZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.246"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            8..565
                     /locus_tag="WHT44_002010"
     CDS             8..565
                     /locus_tag="WHT44_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpA"
                     /protein_id="HEZ2534128.1"
                     /translation="MENNKAKSDDNSVSIEFIQDFITASTDVINLSYENFRKNFYTQM
                     STDSTNYAAKHESLGKSVQRELQKTQSQLRQVVRKMQSKYNINNKARAAEISLLRQMQ
                     SQFSRKDVNKNLGNGNTLAQQGSYTKKDGTTAQAGDLLLAADNLHSRLTDKMLSISHV
                     RENTISPFVLGCLKQITINAYHYLK"
     gene            822..1337
                     /locus_tag="WHT44_002011"
     CDS             822..1337
                     /locus_tag="WHT44_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534129.1"
                     /translation="MSAFRKILLIISCLLIASCSFVETIFYMAISPEPVVVDFPLGKK
                     TKRSIELKQKIGKPYAISLGTNFIHYDPKQGERWIDDKLNYPYNISVKIFKVEEDGKK
                     LIIDELLTERSRKLGGGVFGAGGKYSMYIYDFYLPEGEYLFEISDNSEYIPLYDEINN
                     SIRIVVNARIQ"
BASE COUNT          538 a          201 c          276 g          471 t
ORIGIN      
        1 gcttaatatg gaaaataaca aagccaaatc tgatgataat tcagtttcta ttgaatttat
       61 acaagatttt ataactgcaa gtacggatgt aattaatctg agttacgaaa attttcgtaa
      121 aaatttttat acacaaatgt caactgattc taccaattat gcagccaaac atgaaagttt
      181 aggaaaatcg gtacaacgtg aattacaaaa aacacaaagt cagttgagac aagttgtaag
      241 aaaaatgcag agtaaatata atataaataa taaagcacga gcagcagaaa tatctttgtt
      301 aaggcaaatg caaagccaat tttctcgaaa agatgtaaac aaaaatcttg gtaacggcaa
      361 cactttggct caacaaggca gctacaccaa aaaagacggc acaaccgcgc aagcaggcga
      421 tttgctgttg gctgctgaca acctgcacag ccgcctcacg gacaaaatgc tatccattag
      481 ccatgttcgg gaaaacacga tttccccgtt tgttttaggc tgtctaaaac aaataaccat
      541 aaatgcatat cattatttaa aataaataaa agtatttaac tatttttgac aaaattttag
      601 aaatagagct ttttagttaa gtagaaattg atagtgcttc aagggaagta ttctctatgt
      661 ttgcattaaa gggggtctga taaagctatt attcattact atggactttt atttcattat
      721 tttcaggcgg aaatctcata gccgttttga atttttctct tccttattaa ttatacaaat
      781 aattagtata ttctgatatg gattttttgg aaatttttat tatgtctgca tttagaaaaa
      841 tattattaat aatatcttgc ctattgattg ctagctgcag ttttgttgaa actatttttt
      901 atatggctat tagcccagaa cctgttgtgg tagactttcc tcttggtaaa aaaacaaaaa
      961 gatctattga actcaaacag aaaattggta aaccttatgc aatatcgtta ggaactaatt
     1021 ttatacatta tgatccaaaa cagggggaga ggtggattga tgataagtta aactatccat
     1081 ataatatatc ggttaaaata tttaaagtgg aagaagatgg taaaaaactt attatagatg
     1141 agttgcttac agagagaagt agaaaattag gaggcggagt atttggagct gggggaaaat
     1201 acagtatgta tatttatgat ttttatttgc cggaagggga atatttattt gagatttctg
     1261 ataatagtga atatattcca ctttacgatg aaataaataa ttctataaga atagtagtta
     1321 atgcacgaat tcagtaaatt tttctagaaa tgtggggtta cttatggctg attattatgc
     1381 gataactgta aaatttgcga agcagggtac gccactgaaa caagaggggg tgtatccaag
     1441 acgggtacgt ttgggttgaa ctgtattcgg ctagagataa aaaaat
//
LOCUS       DAWGRZ010000247         1455 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.247, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000247 DAWGRZ010000000
VERSION     DAWGRZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.247"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(1..104)
                     /locus_tag="WHT44_002012"
                     /pseudo
     CDS             complement(1..104)
                     /locus_tag="WHT44_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(141..632)
                     /locus_tag="WHT44_002013"
     CDS             complement(141..632)
                     /locus_tag="WHT44_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ2534130.1"
                     /translation="MKYRRFYRNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRE
                     RYPFEILAWVLMPDHLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRFWEY
                     TIRDEADFAWHFDYLHFNPVKHGYVGQISDWGFSTFHRYVKQGIYPHNWGGGNADFSI
                     GYD"
BASE COUNT          347 a          353 c          382 g          373 t
ORIGIN      
        1 cgggttttcg acaaatatcg gttgcgtttg gtttgcgcct ccgtcagcgg acggttgcgg
       61 caggctttgc gcataatgcc gttctgcaac cgatgctctt tcagttttcc gtaggtcgga
      121 ttctcgaatc cgacattact tcaatcgtat ccaatagaaa agtccgcatt gccgccaccc
      181 caattatgcg gataaatacc ctgtttgaca taacggtgaa acgtagaaaa cccccaatcg
      241 gaaatttgtc ctacatagcc atgtttgacc ggattgaaat gcagataatc aaaatgccag
      301 gcaaaatcgg cctcatcgcg gatagtatat tcccaaaagc gtttttgcca aagcctgaga
      361 ttgccgccga ttaaatattg gctgtgccgc ttgatttgcc gccagcgttc cgaataagca
      421 gaatcattgt ccggcagccg ccatatggta tgcagatggt cgggcatcaa cacccatgcc
      481 aaaatttcaa acggataccg ttcgcgcacc gccattaccg cctgccgtaa agccaaacgc
      541 accgcatcat cggtcaaaat cttctgccgt ttattggtta caaccgtaaa aaagtaagtg
      601 ccgccattgc ggtaaaaacg acggtatttc atagtattat gctcggaatg attttgtagg
      661 tcggattctt gaattcgaca ttttgggcat tgctgcaatg gattgcaatg atgggaatgt
      721 taaaggtttt gtcggataca agtatccgac ctacgcttgc tgaaccgtca ttcccacgaa
      781 agtgggaatc tagaatctcg gggtttcagt catttccgat agattcccgc cgcgtcaggg
      841 ggtctggatt cccgcctgcg cgggaatgac gggtttcaag attgcagtgt tgtcgggaat
      901 gacgggtttc aagattgcgg tgttgtcggg aatgacgaat ccatccatac ggaaacctgc
      961 accacgtcat tcccacggaa gtgggaatct agaatcccgg ggtttcagtc atttccgata
     1021 gattcccgcc gcgtcggggg ttcagtcatt tccgatagat tcccgccgcg tcagggggtc
     1081 tagattcccg cctgcgcggg aatgatgggt ttcaagattg cggtattgtc gggaatgacg
     1141 aatccatcca tacggaaacc tgcaccacgt cattcccacg aaagtgggaa tctagaatcc
     1201 cggggtttca gtcatttccg atagattccc gccgcgtcag ggagtctgga ttcccgcctg
     1261 cgcgggaatg acgaatttcg agattgcggt attatcggga atgacgaatt tcgagattgc
     1321 ggtattgtcg ggaatggcgg gtttcaagat tacggtgttg tcgggaatga cggttcgggt
     1381 atttccacgc ccgccccgcg cctgtaaacg gcaggtgaat caaaaatgcc gtctgaaggt
     1441 tcagacggca tcggt
//
LOCUS       DAWGRZ010000248         1449 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.248, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000248 DAWGRZ010000000
VERSION     DAWGRZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.248"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..138)
                     /locus_tag="WHT44_002014"
     CDS             complement(<1..138)
                     /locus_tag="WHT44_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ2534131.1"
                     /translation="MSTFFQQTAQAMIARHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YL"
     gene            complement(200..871)
                     /locus_tag="WHT44_002015"
     CDS             complement(200..871)
                     /locus_tag="WHT44_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ2534132.1"
                     /translation="MFPDKYKLSLKENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
BASE COUNT          356 a          321 c          345 g          427 t
ORIGIN      
        1 aaggtaacgg gttttttgac ggttcaggta ctgctcgatc ggctgccaat caatcacctg
       61 gtccaacttc aatagcggga aacggtcgat gtgtctggca atcatggctt gggcggtttg
      121 ctggaagaag gtgctcatgg aaaatcccct aaatgtcttg gtgggaattt aaggggtttt
      181 ggggaatttt gcaaaggtct cagtctatgc ccgatataca attttgatac acaaacttgg
      241 aaatatcggt atcgtcgccg gagcgataga atgcggacag tttttcattg aattgcggca
      301 tctgcatttc ggagatttcc aagatgccgc agccccccgc catcatcaga ccgttggcaa
      361 ataaggttgc cgttcgtttg ttgccgtccc aaaaaacctg ctgccgcata caataaagca
      421 taaaacggac ggctgcctcc gtcgtcgaac cgctttgcag tccgatattt tgtaacacgc
      481 gggccacttc aatttccttc actggattcg gggcatgacg ggaaccgtcc aatagcgtta
      541 cgccgaccga accggtacgg aaatcaccgg gtgccaaaga atcgtccttg gcaacaatgt
      601 tgttgatttt tttaaggagt gaaatatcga ccggttcgcc gtttgaaata tgcgaaatca
      661 catattgata ggcacgtttc aggttcaaga tggtttggat gtcttccagt gacgcggagg
      721 ctacattttt gccatggata atttgttcgg tctgcaacaa ggtcgtctga caattctcaa
      781 aacgactgag gttgtgaatt tgggcaacca atactttctt tgccaaaaaa atattttctt
      841 tcaaactcaa cttgtatttg tcgggaaaca cgataattcc ttaaataatt aaagcagtac
      901 gttaaatcat catcttatca tcactttcgg ggtttatgtt gcgtgcgtcc gttttctaat
      961 acggtttccc atcccgcgaa acgaggcgga ggcctgtgca aaaaacctgt tcacgaaatt
     1021 ttccgcatta acgatacgct gaaaatgcgc taaaaatgac tgtgtttgaa tatcggttga
     1081 tttcatccgt ttgtgtaacc tcgcgccggt tttgtcatga ttttgcatta tagtgaatta
     1141 aatttaaacc agtacagcgt tgcctcgcct taccgtacta tctgtactgt ctgcggcttc
     1201 gtcgccttgt cctgattttt gttaattcac tatatttttg tcatgcggat atttcacggg
     1261 atgacaaaac gggcgcaaaa aagcccgatt ggaaaatccg aatcgggctt ttttgtgatg
     1321 ttctgttcga tttgcgtact aactgccggc cggagcgtcc gcgcccggtt tggcaggtcg
     1381 gattctcgaa tccgacggct atttgagatg gcaggctttt gtcggataca agtatccgac
     1441 ctacggctt
//
LOCUS       DAWGRZ010000249         1448 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.249, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000249 DAWGRZ010000000
VERSION     DAWGRZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.249"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            128..1258
                     /locus_tag="WHT44_002016"
     CDS             128..1258
                     /locus_tag="WHT44_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ2534133.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSRGGGGDLERLAARDIIRFPGFVNNVSEVIKEH
                     HIFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAE
                     KMIYFIENREAVRLMGNASYAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT          384 a          344 c          343 g          377 t
ORIGIN      
        1 ggcaacgccg taccggtttt tgttaatcca ctataatacg caggcaaacc caagccttgc
       61 tgggttacag gatgaaaccc tgccgtagca gaccgcattt ccaatccata ttcaaggtgt
      121 ccgccttatg aaaatcgttt ttatcacaac agtcgcatcc agcatttacg gtttccgcgc
      181 ccccgtcatt aaaaaattaa tcggcaaaaa ccatcaggtg tatgcctttg tatcggagtt
      241 ttccgacaat gaattggata ttatcaggga aatgggggtt acacccgtta cctaccgttc
      301 aaaccgcagc gggctgaacc cgttttcgga tataaaatcc accttcctca tctttaaaga
      361 actcaaaaaa atatcgccgg atttggtttt cccttatttc gcaaaacccg tgattttcgg
      421 cacttttgcc gcaaaactgg caggcgtgcc cagaatcgtc gggatgctgg aaggtttggg
      481 attcgcattt accccgcagc cggaaggcat accgttaaaa acaaaaatca taaaggggat
      541 tttgattgcc ttataccgca ttgccctgcc gatgttggaa agcctgattg tattaaaccc
      601 cgacgacaaa gacgaactga cggacaaata cggcatcaaa ataaaaaaca tccatatttt
      661 gggcggaatc ggtctggatt tgcggcaata tccttattcc gaggcggata ttcccgatga
      721 aaaagaaccc gtaaaattcc tctttatcgg cagatttctg aaagaaaagg ggattgatga
      781 ttttattcgg gcggcggaac aggttaagga caaatacccc gatacggttt ttaccgcttt
      841 gggcgcaatc gacaaatcac gcgggggggg gggcgatttg gaacggcttg ccgcccgcga
      901 tattatccgt ttccccggtt ttgtgaacaa tgtttccgaa gtgataaaag aacatcatat
      961 attcgtattg ccgtcttatt atagggaagg cgttccccga agcactcagg aggcaatggc
     1021 cgtcggcagg gcagtgatta cgacggatgt ccccggatgc agggaaacgg tcgccgacaa
     1081 ggtcaacggc ttcctgatcg agccttggaa tccccgcatc ttggccgaaa aaatgattta
     1141 ttttatcgaa aacagggaag ccgtccgcct gatggggaat gcaagttatg cgattgccaa
     1201 agataaattc gatgccgaaa aagtcgattt gaaattgctc gatattttga aggcgtaaac
     1261 aaggctgccc gcttttgagt ttcggtcatt tctgataaac ccccgtcatt cccgccacac
     1321 ccccgtcatt ccctcgaaag cgggaattca ggttcgttta gtttcggtca tttccgataa
     1381 attcttgcaa ctttgcgttt ctagattccc actttcgtgg gaatgacggc ggagggttgc
     1441 tgttttcc
//
LOCUS       DAWGRZ010000250         1440 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.250, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000250 DAWGRZ010000000
VERSION     DAWGRZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.250"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            26..1363
                     /locus_tag="WHT44_002017"
     CDS             26..1363
                     /locus_tag="WHT44_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ2534134.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGKNAVDSFRALRPSTQWLWVGRMGVSACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWLYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
BASE COUNT          266 a          348 c          378 g          448 t
ORIGIN      
        1 atcttcacat ttcttaataa attttatgag taaccatact tcttggtcgt ccaaaatcgg
       61 tttcgtgctt gctgcggcag gttcggccat cggtttgggc gcgatttgga aatttcctta
      121 tacggcaggc accaacggcg gcgcggtgtt tttcctgctg tttttgatat ttaccgtctt
      181 ggtcgcccta cccgttcagc ttgccgaatt ttatatcggg cgcacaggcg gtaaaaatgc
      241 cgtcgattcc ttcagggctc tgcgtccgag tacgcaatgg ctttgggtcg ggcgcatggg
      301 cgtttccgcc tgctttattt tgctgtcgtt ttacagcgtg gtcggcggat gggtattaaa
      361 ttatgtcgtc cacagtttca cgggggcggt tcataccggc gcggactttg aagccctgtt
      421 cggcacaacg atttccaatc cggcaggttc gctgtcctat caggcactgt ttatgctgat
      481 tacggtttgg gtggtcaaag gcgggatttc agacggcatt gaaaaggcaa accgttatct
      541 gatgccgggg ctgtttatcc tctttattgc actggcaatc cgttcgctga cgctgccggg
      601 ggcaatggag ggcgtgtctt tcctgctcaa accgaattgg ttgtacttta aagccgatac
      661 gatgattacg gctttaggcc aggcgttttt tgccttgagc atcggcgttt ccgccatgat
      721 tacctacgct tcgtatttgg gaaaagatca ggatatgttc cgttccggcc atacgattat
      781 gtggatgaac ctcttggttt cgctgcttgc cggcctggtg attttcccgg cggtattcgc
      841 cttcggtttt gaaccgagcc aggggccggg attgattttt atcgtattgc ccgcagtgtt
      901 tatgaagatg ccgttcggta cggttttgtt tgcggtattt atgctgctgg tcgttttcgc
      961 cacgctgact tcggcatttt cgatgttgga aacggtcatt gcctcaacca tccgccaaga
     1021 cgagcgcaaa cgcaaaaaac acacttggct tatcggcacg gccattttca ttgtcggcat
     1081 cccatccgcg ctgtctttcg gcgtatgggg cgagtttaag gttttcggca aaaccatttt
     1141 tgatttgtgg gactatgtta tttccgccgt cattatgccg attggtgctt tgagtgtttc
     1201 catctttacc gcctggattc aggacaagca gtctgtgtta aaagatgccg gcgcgggcag
     1261 caccgtacca cgggcagtgc tgctgctgtg gctgaatacc ttgcgctacc ttgccccgat
     1321 tgccattatt attgttttca tcaattcttt ggacatcctt taaaagccat ccaaacagca
     1381 aaaatgccgt ctgaaagcct ttcagacggc atttttgctt cgggttcagc ctatttcgtt
//
LOCUS       DAWGRZ010000251         1359 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.251, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000251 DAWGRZ010000000
VERSION     DAWGRZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.251"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            255..503
                     /locus_tag="WHT44_002018"
     CDS             255..503
                     /locus_tag="WHT44_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534135.1"
                     /translation="MNIKNEIVALISKKIDGGQYSSLENFALSVLREKIIKNTVSSDE
                     KLLIINDFLSGFLEFDPETGKPVGETLKIEQMIDFFLV"
BASE COUNT          424 a          192 c          296 g          447 t
ORIGIN      
        1 aagtttattg tctttaaatg agataacaga taaaacctta tttaaagaaa atgtagttta
       61 tcgtttcagt aagtattagg tgtgattttt attaagttat ttgaggtttt cggtaagtgt
      121 agatataagg ttgagttata accaatcatt caggctctgt taggcttggc aactcaacct
      181 gtgcttgctg aaattgggta tacagttata cgttttaagc gggcttcgct ccctttatca
      241 tttaagggta atttatgaat attaaaaatg aaatagttgc tttaatttct aaaaaaattg
      301 atggggggca atatagttct ttagaaaatt ttgctttatc cgtactaagg gaaaaaatta
      361 taaaaaatac tgtttccagt gatgaaaaat tattgattat taatgatttt ttatcaggtt
      421 ttcttgagtt tgatcctgag accgggaaac ctgtcgggga gacgcttaaa attgagcaaa
      481 tgattgattt ctttttagtc taaatcatac ataccagtga acaagctagg cttatatccg
      541 gcttttaaat acgattgtca ggtttatact tgggataaaa tcaaaaataa agttgtttaa
      601 actctagtag attaacaaaa atcaggacaa ggcgacgaag ccgcagacag tacagatagt
      661 acggaaccga ttcactcggt gcttcaacac cttagagaat cgttctcttt gagctaaggc
      721 gaggcaacgc cgtactggtt tttgttaatt cactatattg catggtgcag cattaaatca
      781 gtcttctagc gccgcaatgg gaaaacatag aagataaacg aaatggatga tctaatacta
      841 tattttttat caggtatatt cggaaatcaa gttgctgaat gtattataaa aaacaaccgt
      901 gagataaaag ttccctttat tgtgctttac gcaatatttt ttacgttgat ttatactgtg
      961 gctttgttgt ttctcagtct gatctattgg gtcaatggtg cggagattgc atggaagggg
     1021 ataggtattt tcagtatgtc ggtcagtttt tgtatagtct tctgtcttta tttgattgac
     1081 aaggcaggaa gatgtaagga taagaaacaa tagggatcag atgcggcagc aaacgtagat
     1141 tgggtctagg ctcaacctgc gtttgctgca tctttggaag aaatagtgga ttcgaaaaaa
     1201 taggaagaat tctgattaat ggtatgcgag gtgtagcagt ggggactgtt gctggtggca
     1261 taaatggtta tgcaggttct acgggtaaga acactgacat tcgccgttaa ataataaata
     1321 aaccaacttc caaccaaacc tgctatcggt ttggttgga
//
LOCUS       DAWGRZ010000252         1293 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.252, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000252 DAWGRZ010000000
VERSION     DAWGRZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.252"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            41..373
                     /locus_tag="WHT44_002019"
     CDS             41..373
                     /locus_tag="WHT44_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ2534136.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDMPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            705..1214
                     /locus_tag="WHT44_002020"
     CDS             705..1214
                     /locus_tag="WHT44_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ2534137.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT          342 a          247 c          307 g          397 t
ORIGIN      
        1 accttgtttt gacggaaaac catcatataa aggaacactt atgcagattt tatcttttca
       61 accggacatt gcggaacgta tgctggaagg tacggaaggc gagtcggtca acgaaaacgc
      121 acaattcgtc cgtacggaca acggctattg gattgcgtgg catgaaggcg tagcggcact
      181 gcttgcgccc gatatgccgc cgggcattcc ctgtttttgg gtggaaggcg cggaaagcct
      241 tgaagagttg tgcgtcatgg tggaacgcgg cgagtttgac gaagtggaag agtttgacgg
      301 cgatgacgac gaatggctcg aaacggcaca gggttgcggg caccacggcg acgcttgcgc
      361 ctgcggacat taaaggcatt gcaggcttgc cgcaaggggg gcaaggcttt gccgtttttt
      421 aaataatcgg tggattgcat ctgtttgcaa tgggctgaac gcttcccttt gtattttatt
      481 gaataaaaaa acttatcttg gtattataat taaggcagca tcaattattt tgggatggca
      541 ataaacgcaa agcattgatt tgcgccgatt gcagacttat tatagcaggt tgcggcgcgg
      601 acttaatggg aattgtttat agagcatgat ggtctgaaac atcattaatt aaaaatacca
      661 ttaatccgat ttatatttat ttcaatttca atggaaaaac atcaatgaca atgattttaa
      721 gcattttaag cctgtttttt atcatcagac tgttattttt agccgtctct attaaacatg
      781 aaaaagcctt gattgccaaa ggggcgaaac aatacggaaa aaccaattcc acgctgcttg
      841 cggcagttca tacgctttat tatttggcgt gttttgtttg ggtatggctt tctgacactg
      901 cttttaatgg catatccttg attggtacgc tgacggtgat ggcttcgttt gtgatattgt
      961 cattgattat taagcagttg ggggagattt ggacggttaa aatctatatt ttaccaaatc
     1021 atcaaattaa tcgttcgtgg ttgtttaaaa cattccgcca ccccaattat tttttaaaca
     1081 tcatacccga actgattggc atcgccttat tatgtcaagc gtggtatgtt ttattgattg
     1141 gcctgcccat ttatttgctg gtcttattta agcgtatccg acaagaagaa caggcgatgg
     1201 caacactttt ttaacccgtt tcatcaatta tagcggatta acaaaaacca gtacggcgtt
     1261 gcctcgcctt gccgtactgg tttttgttaa tcc
//
LOCUS       DAWGRZ010000253         1280 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.253, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000253 DAWGRZ010000000
VERSION     DAWGRZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.253"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(132..305)
                     /locus_tag="WHT44_002021"
     CDS             complement(132..305)
                     /locus_tag="WHT44_002021"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534138.1"
                     /translation="MAENLPFPSFIHLLFLEFAFTGRGGAPRTPGFGKPPDSAPAELL
                     IEKPFHLAAKGEC"
     gene            270..>1280
                     /locus_tag="WHT44_002022"
     CDS             270..>1280
                     /locus_tag="WHT44_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ2534139.1"
                     /translation="MNKTWKRQVFRHTALYTAILMFSHTGGGGGQAQAQTQTQTHKYA
                     IVMNAQNLPEVKWGDQYQSLTHKSNEREVIHTSGFGLAKKSISFSFNNTDEVVAEKKD
                     TVVFGAATYLPPYGKVSGFDTAKLTERKNALDQIGTTKTGLVGYSYEGSTCSSGGCPT
                     VAYRTQFTFGNSSLAKKTNGGGLDIYEDKSRDNSPIYKLKDHPWLGVSFNLGGESSFK
                     PKRQGSLVSSFSEDVTQQNGAGSQHKDKNLVYTTDDYKSQNNKNHQDKHHAVAFYLNA
                     KLHLLDKKHIKNIVQGKTVNLGILKTRIELTEAWKRRNSNFFNGSWTYEEKGTVSVKL
                     KLP"
BASE COUNT          383 a          291 c          325 g          281 t
ORIGIN      
        1 aggcgggaat ccagaccgtt gggcatctgc agcggtttgc taaaaaccgc tttactgtga
       61 taagtgcgca gggttagaat ggcgcggtaa ccttatatat tgtaccccgt caaaggggcg
      121 cattgctttt cttaacattc ccctttggca gccaagtgaa agggcttttc aatcagcaat
      181 tcggcgggcg cggaatcggg cggtttaccg aaccccggcg ttcgcggcgc gccgccccgt
      241 cccgtgaagg caaactcaag gaataaaaga tgaataaaac ttggaaacgg caggttttcc
      301 gccataccgc gctttatacc gccatattga tgttttccca taccggcggg gggggggggc
      361 aggcgcaagc gcaaacgcaa acgcaaacgc ataaatacgc tattgtaatg aacgcgcaaa
      421 atctgcccga ggtaaagtgg ggggatcaat atcagtcatt gacgcacaaa agcaatgaac
      481 gcgaagttat ccatacgagt ggttttggtt tggcaaaaaa gagcattagt ttctcattca
      541 ataataccga tgaagttgtt gctgaaaaaa aagatactgt cgttttcggc gcggcgacct
      601 acctgccgcc ctacggaaag gtttccggtt ttgataccgc taagctgacc gagcgcaaaa
      661 atgcccttga tcagattggt acgaccaaaa cggggctggt aggctacagc tacgaaggta
      721 gcacatgctc cagcggaggt tgtcctacag ttgcctatag aacccaattt accttcggca
      781 attccagttt ggcaaaaaag acaaacggcg gcgggctgga tatatacgaa gacaaaagcc
      841 gcgacaattc gcccatttac aaattgaagg atcatccttg gttgggcgtg tctttcaatt
      901 tgggcggaga gagctccttc aaaccaaaga gacaaggttc tttggtatct tcttttagcg
      961 aggacgtgac gcagcaaaat ggtgcgggca gccaacacaa agacaaaaac ctcgtttata
     1021 cgacagacga ttacaagagt cagaataata aaaaccatca ggacaaacac cacgccgtcg
     1081 ccttttatct gaacgccaag ctgcacctgc tggataaaaa acacattaaa aatatcgtgc
     1141 aaggtaaaac agttaatttg ggtatcttga aaacacgcat cgagctgacg gaagcatgga
     1201 aaagacggaa tagtaacttt tttaacggta gttggacgta tgaagagaaa ggaacagtca
     1261 gcgtcaaact caaattgccg
//
LOCUS       DAWGRZ010000254         1271 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.254, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000254 DAWGRZ010000000
VERSION     DAWGRZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.254"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..328)
                     /locus_tag="WHT44_002023"
     CDS             complement(<1..328)
                     /locus_tag="WHT44_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534140.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNES"
     gene            complement(366..710)
                     /locus_tag="WHT44_002024"
     CDS             complement(366..710)
                     /locus_tag="WHT44_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534141.1"
                     /translation="MFLDDVNVFLDDLNVFLDDLNTNPITDEWYMSNFADKHIKILES
                     YEAFDILKQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQ
                     EISQDILNEIFR"
     gene            complement(713..901)
                     /locus_tag="WHT44_002025"
     CDS             complement(713..901)
                     /locus_tag="WHT44_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ2534142.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENSRTGEKTDFKFKD"
     gene            complement(971..1271)
                     /locus_tag="WHT44_002026"
                     /pseudo
     CDS             complement(971..1271)
                     /locus_tag="WHT44_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
BASE COUNT          350 a          224 c          195 g          502 t
ORIGIN      
        1 gggattcatt tttaataata tcttcaagca agtgaaatgt aagaaaagcg tagatacttg
       61 aaggccggct atctgattgg caaaaagaaa tttcggagat aaagaatttc cgtttatatt
      121 tttcataaat taaatcaaca ccttgtttta cataaaaact aacttcctca ggaaggatat
      181 ttataacatt ctctcctttt acttttatag ttaatggttt gatttttatg ttttcatgaa
      241 tatctgatag gattatttct aaaaaattat ttttatttcc agtcattctg gctattttat
      301 gtatatttcc attgtgttcg attatcattt tattttttcc aaggatttcc ttctatattg
      361 atagtttatc taaagatttc atttaaaata tcctgactaa tttcttgttt atataattct
      421 acaattttct gtttctgtgt atttgtataa aatttttcgt tggtatctgc ttgatatttt
      481 aattgtctca atatttccat gatttcatat tctgattttt catcatattc ttcaatcatg
      541 taatcaacaa attgttttag aatatcaaag gcttcgtaac tttccaaaat tttaatatgt
      601 ttatcggcaa aattggacat ataccattcg tcagtgattg gattagtatt taaatcatct
      661 aaaaaaacat ttaaatcatc taaaaaaaca tttacatcat ctaaaaacat gactaatcct
      721 taaacttaaa atctgttttc tcaccggttc ttgagttttc aatgtaatgt atttcaattt
      781 tgtttacgtt ttgaacacgc ttaacccaac cgtcttttgc taaccaacca tttttagtat
      841 cggacatagg aggtattctc gcaggagttt tgccctgtgg gtttgcctta acttcttcca
      901 ttgctaattg ttcctttaaa ttattaggta ttgtacgacc ggttgatttt tcaacagatt
      961 gtgtccctat ttttaatgat ttgtttcccc cagccatttc cccgtctaca gtctgcatag
     1021 ggcgggcagc aggtttttta tagccggtat ttttaaccca atcccattta gtcgggttaa
     1081 gttctacttt tttaccgcgc caaaccgtac ctgcggcctc tgctacggaa agggcagttt
     1141 gggctgttgc agttatattc ggatgggcat cagcccattg tctggcggaa ttaatgccgt
     1201 tttttaccgc aaaagcactg tcctgtaata ggctcgcggc agcaagttgt gcttccggac
     1261 ttaaatttcc t
//
LOCUS       DAWGRZ010000255         1258 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.255, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000255 DAWGRZ010000000
VERSION     DAWGRZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.255"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(85..1188)
                     /locus_tag="WHT44_002027"
     CDS             complement(85..1188)
                     /locus_tag="WHT44_002027"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ2534143.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERASILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
BASE COUNT          285 a          349 c          341 g          283 t
ORIGIN      
        1 tttccgacag ccgaacaaaa ccccgaatcc gtctgcacgg ttcggggtat atctccaata
       61 cgggcatcgt gttccggaaa accgtcaaat ccgcatcggc atcacaatat atttgaaatt
      121 cggattgttc ggcacggtaa acagcgtcga gcggttggca tcgccgaagg caagctgcat
      181 atcgtcggaa tggatgttgc gcaacacgtc catcagatag ccgatattga aaccgacttc
      241 gagttcgccg ccctgatagg cgatttcgat ttcttcgcgc gcttcttcct gctcgttgtt
      301 gctgcacaca acgctcaaca ggccgggttg caaaaacaat cgcgcaccgc ggaatttttc
      361 attggcaaga atcgatgcac gttccaacgc gcccaacaat tctgccctcg acaacacgaa
      421 aatcttgtcg ttgtccaaag gaatcacgcg gttgaaatcg gggaatttgc cgtcgatgac
      481 cttgctgacg atggtcgtgc cgttgcattg gaaacgcacc tgtttgtcca gcagctcgat
      541 ttgaatcgga tcgtcggggt tgttcaacag tttgaacagt tccagcaccg ttttgcgcgg
      601 caaaatcact tcggcgcgcg gcaaatccgc atcaatcgcg caggctgcat aggcaaggcg
      661 gtgtccgtcg gtcgccacaa ggcgcaactg gctgccctca acctgcatca gcagaccgtt
      721 gagataatag cggatgtcct gcaccgccat gctgtactgc acttgcgaca gcatggtttt
      781 gaaacgctcc tgctccagcg agaaagtcgc gctgatgtcc tcgccgacat tcatcatcgg
      841 aaaatcggcg gcaggcaggg tctgcagggc aaaacgcgat ttgcccgcct tcagcgtcag
      901 acggctgtcg tcccaatcca gcgacaccag cgcaccggca ggcagcgcgc gcaaaatatc
      961 ctgaaatttc ttggcattgg tggtgatgcg gaagtcgccc gcgccgccct cgggacccgc
     1021 agtgtcgatt tggatttcca aatcggttgc caagagtttg gtctgaccgc cttttccctc
     1081 aatcaggacg ttggacagga tgggcagggt gtggcggcgt tcgacgatgc cggtaacggc
     1141 ttgcaacggc ttgagcaggc tgtcgcgctc ggcttgtaaa atcaacatgt tcgctccttt
     1201 aaatcggttt gtatagtgga ttaaatttaa atcaggacaa ggcgacgaag ccgcagac
//
LOCUS       DAWGRZ010000256         1217 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.256, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000256 DAWGRZ010000000
VERSION     DAWGRZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.256"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            321..491
                     /locus_tag="WHT44_002028"
     CDS             321..491
                     /locus_tag="WHT44_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ2534144.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT          378 a          337 c          272 g          230 t
ORIGIN      
        1 ccctaacgca acaggaattc atcgaaaaaa ccgaaacctc caaaccgtca ttcccacgga
       61 agtgggaatc cagtaatcga aaaaccacag gaatctatcg aaaaaaacca accccaaaaa
      121 aaccgggcgg ataccgcacc attctcccaa accctgattt atagcggatg aacaaaaatc
      181 agggcaaggc ggcgaagccg cagacagtac aaatagtacg ggtgcttcag tactttagag
      241 aatcgttctc ttcgagctaa ggcgaggcaa cgccgtaccg gtttttgtta acccactata
      301 caaaaaacaa ggaaacacaa atggcgcaat atatgtgcgg cccctgcggc tggatttacg
      361 atgaagaaca cggcgacccc gaacacggca tcgcccccgg aaccaagttt gaagacatcc
      421 ccgacgactg gaaatgcccc gaatgcggcg tgggcaaaga agatttctac ctgttggatt
      481 tcgtgatata gcggcagccg cacccttagg gaaaaccccg tcatccggcg ttccgcctgc
      541 ggcggcaggg aacaccgttt tatagtggat taacaaaaat catgacaagg cgacgaagcc
      601 gcagacagta caaatagtac ggaaccaatt cacttgttaa agaatcgttc tctttgagct
      661 aaggcgaggc aacgccgtac tggtttttgt taatccacta tatccgtttg cgcccgccaa
      721 gccgtgttgc acagatgcga cacggctttt tccttttcgt cgtccgaatg taaattttcg
      781 gcaaaaaacg tcaaaaatca cccccccccc gcatttgcgt aaaataaagt gtaatccctt
      841 tgatcataag ggaatgttga caattagatc gattctccta ggctgacttt cgcaataaat
      901 ggggttgtca ataattgtgt atggtcgttt gccgtccggc ggccgtcatt cccgcgcagg
      961 cgggaatcca gcaacacaac gcagcaggac tttatcggaa aaaaccgaaa ccccaccgac
     1021 cgtcattccc gcaaaagcgg gaatccaaaa acgcaacgca acaggaattt atcggaaaaa
     1081 accgaaaccc caccgccgtc attcccgcaa aagcgggaat ccagcaaccg aaaaaccaca
     1141 ggaatctatc agcaaaaaca gaaaccccac cgccgtcatt cccgcgaaag cgggaatcca
     1201 gacccgtcgg cacggaa
//
LOCUS       DAWGRZ010000257         1210 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.257, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000257 DAWGRZ010000000
VERSION     DAWGRZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.257"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..525
                     /gene="mafB"
                     /locus_tag="WHT44_002029"
     CDS             <1..525
                     /gene="mafB"
                     /locus_tag="WHT44_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ2534145.1"
                     /translation="ITATAQTALAVAEAATTVWRGKKVELNPTKWDWVKNTGYETPAV
                     RHMQTMDGEMAGGNRPPKSITSNGKADASTQPSLQAQLIGEQISSGHAYNKHVIRQQE
                     FTDLNINSPADFARHIENIVANPSESKKLSNGRSAYWDDKSGTVVIRNPKDPDGGTAF
                     RPKIGKQYFDDKLK"
     gene            538..747
                     /locus_tag="WHT44_002030"
     CDS             538..747
                     /locus_tag="WHT44_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534146.1"
                     /translation="MKLIGFEENLVKISLNKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKNKVLSLDKELKKIYDTWDKC"
     gene            856..1089
                     /locus_tag="WHT44_002031"
                     /pseudo
     CDS             856..1089
                     /locus_tag="WHT44_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          429 a          188 c          257 g          336 t
ORIGIN      
        1 ataactgcaa cagcccaaac tgcccttgcc gtagcagagg ccgcaactac ggtttggcgc
       61 ggtaaaaagg tagaacttaa cccgaccaaa tgggattggg ttaaaaatac cggctatgaa
      121 acacctgctg ttcgccatat gcagactatg gatggggaga tggcaggtgg gaatagaccg
      181 cctaaatcta taacgtccaa cggcaaagca gatgcttcca cacaaccgtc tttacaagcg
      241 caactaattg gagaacaaat tagtagtggg catgcttata acaagcacgt cataagacaa
      301 caagaattta cggatttaaa tatcaattca ccagcagatt ttgctcggca tattgaaaat
      361 attgttgcca atccttccga aagcaaaaaa ttatccaatg ggcgtagtgc atattgggac
      421 gataagtctg gaacggttgt aattagaaat cccaaagacc cagatggtgg gacagcattt
      481 agaccaaaaa taggtaaaca gtattttgat gacaaattaa agtaaaagga attattcatg
      541 aaattaattg gttttgaaga gaatttagtt aaaattagtc tcaataaaga tgagttgtat
      601 attattcaag caattgtagg agaaatttat tcaggcgtat gcgtggattg tagagatttt
      661 gagataattc atggtgttga aaaaaataaa gttctttctt tggataaaga acttaaaaaa
      721 atatatgata cttgggataa atgttagcta ggtagggtgg gcactcgttg cccacctttt
      781 gccgtatatt aaaaatcctt ttcaggctgg aactgagtgg gcatcaatgc caaccctaat
      841 tgtgctgatg aagcagtatt aagacaagca tcgaatggtg attatcatgg ttttccgcaa
      901 tcagtagatg ctttttctga aaatggaaca gtaatacaaa tagttggcgg agataatatt
      961 gtaaggcata aactttatat ccctggatct tataaaggaa aagatggaaa ttttgaatat
     1021 atcagagaag cagatggaaa aattaatcat agattatttg tccctaacca acaacttcct
     1081 gaaaaatagt agatagagat taatatgtta aatgaaattt ttgaaattta ttcgagacaa
     1141 ggggaatctt tgataggaat tggaattaga gaagccgcat tacccgtccc tattgcaata
     1201 gatatattaa
//
LOCUS       DAWGRZ010000258         1208 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.258, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000258 DAWGRZ010000000
VERSION     DAWGRZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.258"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(179..538)
                     /locus_tag="WHT44_002032"
     CDS             complement(179..538)
                     /locus_tag="WHT44_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534147.1"
                     /translation="MASYVSIKGWIECSDDDIKIIQENINNFWNNCPFNIEEKESAKI
                     YKSGWVFPTNSFNWSSYIFFGACVKSYFIIYFEKCIKTIMELDIEISGFFELDYYEDN
                     YKVNWKINNGNLIQTTN"
     gene            complement(541..>1208)
                     /gene="mafB"
                     /locus_tag="WHT44_002033"
     CDS             complement(541..>1208)
                     /gene="mafB"
                     /locus_tag="WHT44_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ2534148.1"
                     /translation="GIGDILYGTRYAIDKAAMRNIAPLPAEGKFAVIGGLGSVAGFEK
                     NTREAVDRWIQENPNAAETVEAVFNVAAAAKVAKLAKAAKPGKAAVSGDFADSYKKKL
                     ALSDSARQLYQNAKYREALDIHYEDLIRRKTDGSSKFINGREIDAVTNDALIQAKRTI
                     SAIDKPKNFLNQKNRKQIKATIEAANQQGKRAEFWFKYGVHSQVKSYIESKGGIVKTG
                     LGD"
BASE COUNT          320 a          252 c          197 g          439 t
ORIGIN      
        1 aagaacttag ttctcaatat cctgtttcat tcatcaaaat gccgtctgaa agctgaatac
       61 cgcttcagac ggcattttgg tggttgggtt tttaagccaa cctacgctta ctgaaaacca
      121 aattgagttt cagacagttt ttaggtttgg gtgtccaatc taacttatat ttgtccattt
      181 aattagtcgt ttgtatcaaa tttccattat ttattttcca atttacttta taattatctt
      241 cataataatc taattcaaaa aaacctgata tttcaatatc caattccatt attgttttaa
      301 tacatttttc aaaataaata atgaaataag attttacgca tgcaccaaaa aaaatatagc
      361 tgctccaatt aaaactattt gtcgggaaaa cccacccgct tttatatatt tttgcagatt
      421 ctttctcttc gatattaaag ggacaattat tccaaaaatt attaatgttt tcttggatga
      481 tttttatatc atcgtcagag cattcaatcc atccttttat ggaaacatat gatgccatgt
      541 ttaatctcct aaacctgttt taacaatgcc gccttttgat tcaatatatg acttaacttg
      601 tgaatgaaca ccgtatttaa accaaaattc tgcacgtttt ccctgttggt ttgctgcttc
      661 gatggttgct ttaatttgct ttctattttt ttgatttaag aaatttttag gtttatctat
      721 tgctgaaatt gttcttttgg cttgtattaa agcatcattc gtaacagcgt caatttctct
      781 gccgttaata aattttgatg aaccatcagt ttttcttcta attaaatctt cataatgtat
      841 atctagagct tctctatact ttgcattttg atataactgt ctcgcactat cagacaaagc
      901 caatttcttt ttataagaat cagcaaaatc cccgctaacc gcagccttcc ctggttttgc
      961 cgcctttgcc aacttcgcga ctttggctgc tgcggcaacg ttgaagacgg cttcgacggt
     1021 ttcggcggca ttgggatttt cctgtatcca ccggtcaacg gcttcgcgcg tattcttttc
     1081 aaagcccgcc acgctgccca agccgccgat gacggcgaat ttgccctcgg cgggcaaggg
     1141 ggcgatgttg cgcattgcgg ctttgtctat ggcatagcgc gttccgtaca gtatgtcgcc
     1201 tatgccca
//
LOCUS       DAWGRZ010000259         1201 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.259, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000259 DAWGRZ010000000
VERSION     DAWGRZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.259"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..347
                     /locus_tag="WHT44_002034"
     CDS             <1..347
                     /locus_tag="WHT44_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ2534149.1"
                     /translation="RGSDVAIEFHTNAAANKTATGIEALSTPKNKRWCQVLGKAVAKK
                     TGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTTKWGICRAIA
                     DAIAMELGAAKV"
     gene            344..547
                     /locus_tag="WHT44_002035"
     CDS             344..547
                     /locus_tag="WHT44_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534150.1"
                     /translation="MKKSLIALCVAHCAKLKNDFGVPPLPEIKITPSPVRVGSLKQHP
                     SLRLGKSGVAAAKRAARKRKNRR"
     gene            550..714
                     /locus_tag="WHT44_002036"
     CDS             550..714
                     /locus_tag="WHT44_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534151.1"
                     /translation="MGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYREML
                     KRHLQTKSVE"
     gene            717..953
                     /locus_tag="WHT44_002037"
     CDS             717..953
                     /locus_tag="WHT44_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534152.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSFVSVKRQEVENESRETAGE"
     gene            925..>1201
                     /locus_tag="WHT44_002038"
     CDS             925..>1201
                     /locus_tag="WHT44_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534153.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTGLAVSHHQGYKSAFAKQQAVID
                     KMERDKAQALLLSAQNYARELELARAEAKKYEVKAHAVGMALAKKQAE"
BASE COUNT          295 a          274 c          373 g          259 t
ORIGIN      
        1 ttcgcggctc ggatgtggcg attgagttcc acaccaatgc ggcggcgaac aaaacggcga
       61 caggcatcga agccttgtcc acgccgaaaa ataaacgctg gtgtcaggtg ctgggcaaag
      121 ccgttgccaa gaaaaccggc tggaaactgc gcggcgaaga cggctttaag ccggataacg
      181 cagggcaaca ttcgcgcctg gcttatgcgc aggcaggcgg cattgtgttt gagccttttt
      241 tcatcagcaa cgacactgat ttggccttgt ttaagacgac caaatggggc atctgccgcg
      301 cgattgcgga cgcgattgcg atggaattgg gagcggcgaa ggtatgaaaa agtctttgat
      361 tgctttatgt gttgcccatt gtgcaaagtt gaaaaacgat tttggcgtac caccgttacc
      421 tgaaatcaaa atcacgccaa gccctgttcg ggtaggctct ttgaaacaac atccgagcct
      481 gcgcttgggt aaatcaggcg tggcggctgc taaacgtgcg gcgcgcaaac gcaagaatcg
      541 tcgttaatca tgggacaggt tgcgttttac gaaaagatga ttgggctgtg gtcggccaaa
      601 agccgtgagg caagcgaaca ggcggacttg gctgcgtttg aatttgcgga gggcgaactg
      661 gccaattatc gggaaatgct gaaacggcac ctgcaaacca aaagtgtgga atagcaatgc
      721 gtattttgga tatttttaaa aacccggcga caggcaatgt gtcgcactcg aaactgtggg
      781 caaacgttgc ctgcgcggct gggacgttta agtttgtgat gttgcccgat ccgtcggcgg
      841 aaatttgggc ggtgtatttg ggcattgtcg gcggctatgc ggtggcgcgt tcatttgtca
      901 gcgtgaagcg tcaggaggtc gagaatgaat ctcgtgaaac tgctggcgaa taactggcaa
      961 ccgattgcca ttatcgcgct tgtcggcacg ggcttggctg tgtcgcacca tcaaggctac
     1021 aagtcggcat ttgcgaagca gcaggcggtc atcgacaaga tggagcgcga caaggcgcaa
     1081 gccctgctgt tgtcggctca aaactatgcg cgcgaactgg aactggcacg cgcggaagct
     1141 aaaaaatatg aagtcaaggc gcacgctgtc ggcatggctt tggcgaaaaa acaggcggaa
     1201 g
//
LOCUS       DAWGRZ010000260         1191 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.260, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000260 DAWGRZ010000000
VERSION     DAWGRZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.260"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(4..462)
                     /locus_tag="WHT44_002039"
     CDS             complement(4..462)
                     /locus_tag="WHT44_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534154.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     SYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
BASE COUNT          428 a          215 c          123 g          425 t
ORIGIN      
        1 cgtttagtct agagtctgta tattacattt atttttaggg tctgctagcc aatttcttgt
       61 tcccttcatt attttatctt ctgaaagaaa attatttttt tccatgctat taatattaat
      121 gatatgattt ttatttaaaa taatttttcc atatgaaatt tccggtttgt aatagcaaga
      181 gtctgaagca aatccttgcc cgtcaatata agaaaaataa atgttatcat cctccttata
      241 atattctaaa accaatccgg atattatttt atcaaaattc ccttcatttc catctttcat
      301 gactaaaata taattagcag gccaaccttt atcgtaaaca tattcaaact gatagttccc
      361 gaaactctcg atatccgaac taaaaaagaa gaaaagcaaa ttaatagcca atacaaaaac
      421 aaataagaac aaaaatagca aaattttcaa cttagttaac aatatttacc tctcctttaa
      481 attcaatcct gaaaggtacc ccttaccctg ggctaccaat tatagtttcc atatttctaa
      541 atattgtttt tacattactt ttttctcccc ccaaaggaat gcattttaaa atcatgcttt
      601 tcaggtgcta atcgatactt accattaaca tctttaatca cagatatatt tccatgtata
      661 gcacaacgtg aaaaatctga gtattatata cagttatacc ttactgaaac aaagctagct
      721 gtacatatct gtaccatatt tttactacaa acaaaaaata tttataatta ttaaggataa
      781 gataaaaacc cccaacgcca gattaattct taataatttc aaataaaaaa tatttgagat
      841 tttttctttt ccaaataata tattgaaata tttaaagtta aggtagcttg atttaattag
      901 aaaatcaaaa aaaccattaa ttaatttttc ttctttaaat attttatatg atattataaa
      961 attaaataac attgcgaaaa catatgaaat catcaaaata ccaaatatat agtgggaatg
     1021 cattattatt tagctccttc ttttggcttt gacaaattga cagtccattg atatccaatg
     1081 cttgcatttg caattgctga cgcacctgcc cctgttttcc ctgttagtat caatgattct
     1141 tttcttgcaa gatgtgcacc tgcacaacca cctaatacta aaccaccacc t
//
LOCUS       DAWGRZ010000261         1151 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.261, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000261 DAWGRZ010000000
VERSION     DAWGRZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.261"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            81..1046
                     /locus_tag="WHT44_002040"
     CDS             81..1046
                     /locus_tag="WHT44_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ2534155.1"
                     /translation="MLRLTALTLCTVLALGACSPKDSDSAPQAKEQAVSAAQSEGASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNLLPYLDEYFKTTKPA
                     GTLFEPDYEALNAYKPQLIIIGSRAAKAFDKLNAIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDESIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLDNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
BASE COUNT          290 a          321 c          303 g          237 t
ORIGIN      
        1 tatcatttat cctttctaaa gccgttccgg tttgtccgac cggcggcttt gccccaatat
       61 ccccattttg gagacaccct atgttgcgtt tgaccgcatt gaccctctgc accgtcctcg
      121 ccttgggcgc gtgttcgccg aaagattccg actctgcccc acaagccaaa gaacaggcag
      181 tttccgccgc acaatccgaa ggcgcgtccg ttaccgtcaa aaccgcgcgc ggcgatgttc
      241 aaataccgca aaaccccgaa cgcatcgccg tttacgattt gggtatgctc gacaccttga
      301 gcaaactggg cgtgaaaacc ggtttgtccg tcgataaaaa cctgctgcct tatttggacg
      361 aatatttcaa aaccacgaaa cctgccggta cgctgtttga gccggattac gaagcactca
      421 acgcttacaa accgcagctc atcatcatcg gcagccgcgc agccaaagcg tttgacaagt
      481 tgaacgccat tgcgccgacc atcgagatga ccgccgatac cgccaacctg aaagaaagtg
      541 ccaaagaacg tatcgacgcg ctggcgcaaa tcttcggcaa acaggcggaa gccgacaagc
      601 tgaaagcgga aatcgacgcg tcttttgaag ccgcgaaaac tgccgcacaa ggcaaaggca
      661 aaggtttggt gattttggtc aacggcggca agatgtcggc tttcggcccg tcttcacgct
      721 tgggcggctg gctgcacaaa gacatcggcg ttcccgctgt cgatgaatca attaaagaag
      781 gcagccacgg tcagcctatc agctttgaat acctgaaaga gaaaaatccc gactggctgt
      841 ttgtccttga ccgaagcgcg gccatcggtg aagagggtca ggcggcgaaa gacgtgttgg
      901 ataatccgct ggttgccgaa acgaccgctt ggaaaaaagg acaggtggtt taccttgttc
      961 ctgaaactta tttggcggcc ggcggcgcac aagaattgct gaatgcaagc aaacaggttg
     1021 ccgacgcttt taacgcggca aaataatgaa acggcggcat tcgatgccgt ctgaaacacg
     1081 gatgcaaacc gcctcctgtg tttcagacgg cattgcccga tacgggcgtt atctcgggaa
     1141 tggcggcaag c
//
LOCUS       DAWGRZ010000262         1146 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.262, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000262 DAWGRZ010000000
VERSION     DAWGRZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.262"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..425
                     /locus_tag="WHT44_002041"
     CDS             <1..425
                     /locus_tag="WHT44_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ2534156.1"
                     /translation="PTQTVKELDGLLQESKNIGAVNTRINIANSTTRYTPMRQTGQPV
                     SAGFEHVLEGHFHRPIANNRSVFTISPNELKVILQSNKVVSSPVSMTPDGQYMRTVDV
                     GKVIGTTSIKEGGQPTTTIKVFTDKSGNLITTYPVKGN"
     gene            432..941
                     /locus_tag="WHT44_002042"
     CDS             432..941
                     /locus_tag="WHT44_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534157.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
BASE COUNT          399 a          197 c          196 g          354 t
ORIGIN      
        1 taccaaccca gacagttaaa gaacttgatg gcttactaca agaatcaaaa aatataggtg
       61 ctgtaaatac acgaattaat atagcgaata gtactactcg atatacacca atgagacaaa
      121 cgggacaacc ggtatctgct ggctttgagc atgttcttga ggggcacttc cataggccta
      181 ttgcgaataa ccgttcagtt tttaccatct ccccaaatga attgaaggtt atacttcaaa
      241 gtaataaagt agtttcttct cccgtatcga tgactcctga tggccaatat atgcggactg
      301 tcgatgtagg aaaagttatt ggtactactt ctattaaaga aggtggacaa cccacaacta
      361 caattaaagt atttacagat aagtcaggaa atttgattac tacataccca gtaaaaggaa
      421 actaactaaa tatgagtaac tttgaaaaaa aatatatttt agaattaaat gatgctttaa
      481 gccatttaaa tcataactct acctcatttg atttattgaa agttttgatt tcatggttat
      541 caaacgatat tgtcattgat aaatttaaaa ttttaggtta tgactttagt aaatatatcg
      601 aaatgaatcc cgatgactat ccggttgaaa aatctatatt gaatagagag gaaattattt
      661 atctcaaaaa caatatttat cgtaaaatat catcaggaaa ttttaaattt caatactttg
      721 tacaatatat tagagatatt ttagaatatt tatttattga acatattgaa agagtctgtc
      781 cttactgcga atggggtgaa atgcaaaaat tagaagaaca aaatacgcat gaaacggtgt
      841 atctctgtac tcaatgtgga tgtgcttttt ataacgataa ttcacaattt ttattaaaaa
      901 cccctttaac cattccaatg aaacgtgatg aatttaaata aacaagccgt agcctgcatg
      961 aaccctaaaa tccacgtgta gcgtgtgtgc gccagcacgc atgcgttcca tgatttacgg
     1021 ctcaatgccg tctgaaaagc tcacaatttt tcagacggca tttgttatgc aagtaaatat
     1081 tcagattccc tgtatgctgt acagacgcgg gagtgttaag ccccccttgt ttgaagctcc
     1141 gcggct
//
LOCUS       DAWGRZ010000263         1145 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.263, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000263 DAWGRZ010000000
VERSION     DAWGRZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.263"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(129..398)
                     /locus_tag="WHT44_002043"
     CDS             complement(129..398)
                     /locus_tag="WHT44_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534158.1"
                     /translation="MMTESHGYLSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYCWLGFDELPQSEKIKFLSYLNILSIHKEIQDETVNRVYTD"
     gene            complement(419..625)
                     /locus_tag="WHT44_002044"
     CDS             complement(419..625)
                     /locus_tag="WHT44_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534159.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKDLPIR"
     gene            complement(591..791)
                     /locus_tag="WHT44_002045"
     CDS             complement(591..791)
                     /locus_tag="WHT44_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534160.1"
                     /translation="MASDLLSKQTSDKILGYQTEHRRVRYDINNNIYVLANPKTFKIK
                     TMFKPNLGKKYYDGEFKKDMGN"
BASE COUNT          310 a          221 c          186 g          428 t
ORIGIN      
        1 ttaatatatc tattgcaata gggacgggta atgcggcttc tctaattcca attcctatca
       61 aagattcccc ttgtctcgaa taaatttcaa aaatttcatt taacatgtta atctctatct
      121 actatttttc aatcggtata aaccctattc acagtttcat cttgtatttc tttatgaata
      181 cttaatatat taagatagct taggaatttt attttctcag attgaggcaa ctcatcaaaa
      241 ccaagccaac aataaaaatt gttatatgat tttaaaaaat caacaatttt tatttttcct
      301 aaaaccaaat cttcgactaa ctgggttgca taaataatat catttatgat tttaacaggt
      361 aaattatttg ataggtaacc atgggattcc gtcatcattg atttctctag gtgcttattt
      421 atctgattgg taagtctttt gcgtaagctt ctttcgcctc cgcaagtgtc atttcattag
      481 ggccaccatc tatattagtt tctcctgtat tttgccactg acaaacgtca cagatatcat
      541 agtccataac ttcagttccg caaacaggac aatgtagcca tatttctccg tcaatttccc
      601 atgtcttttt tgaattctcc atcataatac ttctttccta agtttggttt aaacattgtt
      661 ttgattttga atgtttttgg attggccaaa acatagatat tgttattgat atcatagcgc
      721 actcgtctat gttccgtctg ataacctaat atcttgtccg atgtctgttt tgataacaaa
      781 tcagacgcca ttttttggta gtcttcaatg gttatatcgc caaattcttt tccatgagct
      841 ttgaaatgtc catttaaaga tttctcagta ggaaacttgg actttgtcca tctgatgcgg
      901 tcttgaattg ttcctgtcgg agaaatagat gtatcaggtt taacatatga ggcaggtaag
      961 ccgatattgt tatcagagtg tgtagggtgt tgctgcgtac ttggttttgg tggcttattc
     1021 ccacctgcca tctccccatc taaagtctgc atagggcggg cagtaggtgt tttatagccg
     1081 gtatttttaa cccaatccca tttggtcggg ttaagttcta cttttttacc gccccaaacc
     1141 gtacc
//
LOCUS       DAWGRZ010000264         1128 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.264, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000264 DAWGRZ010000000
VERSION     DAWGRZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.264"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(231..1085)
                     /locus_tag="WHT44_002046"
     CDS             complement(231..1085)
                     /locus_tag="WHT44_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ2534161.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          314 a          339 c          294 g          181 t
ORIGIN      
        1 tcccgtcatt cccgcgcagg cggaaatccg gacatccaac gctgcagcaa tttgtcggaa
       61 ataaccgaaa ctcaaaaaac cggatgcccg cctgcgcggg aatgacgacg caaagatggt
      121 cgttatttcg gataaatatc cgtcttttcg ttccggatac ccgctttcgc aggaatggag
      181 atggaaagat ttcagcctat tcggcaatca tcaaataccg cttcccaact ttatttttga
      241 tgcgacagcc ggccggcaac caaggtggcg gcgatgacga caaacacgcc caaggcggac
      301 acgggcgaca ggtgttcgcc caaaatcaaa accgccagca gcacgccgac gacgggttcg
      361 agcgaaatca acagtcccga aacattggca ggaacacggc tcatcccctt gttccacagc
      421 caataggcgt accagccgca ccccaaaccc aaatacagca gcgacaatac catcccgacg
      481 ctccagtcca cggtataact ttgcgccaaa gcaagcgaaa acggcaggca catcaacgat
      541 gcggcggcaa tggaaacaga tgtgaatgcc ggtgcgccga tgcgtgcaat cagcctttgc
      601 gtcggacgca tagcggcaca aaagcccgcg cccgccaaca acaccagcag gcagccgaac
      661 cagccgactt cgccgccctc ttccgcaccg cccgccatca gcagcgcgac accggcaaat
      721 gccgccgcgc cgcatatcca gtggtaggca cgcgctttgt cgttgaagaa aaagtgtccg
      781 acaaacacca tcagcagcgg ctcgagtccg acaatgaccg atgcgctggc ggcggaagtg
      841 tatttcaacc cgacaaactg aagcagcagg gtcagcacat agttgacgaa cgacacaatc
      901 agcaacggct tccattcctc acgcggaatc ttgccgacat gacggcggca ggcgggcagt
      961 gcaggcagcg cggcaattag caggcgcacg ccgaccatca atgcgggatc gatgccgcca
     1021 tagacatatt tggcggcaat aaacgagctg ctccagataa tcagggcaag gatttggtaa
     1081 aacatagtga gtgataaagt cagatggtca tacaaaagta tagcggat
//
LOCUS       DAWGRZ010000265         1039 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.265, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000265 DAWGRZ010000000
VERSION     DAWGRZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.265"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            12..1013
                     /locus_tag="WHT44_002047"
     CDS             12..1013
                     /locus_tag="WHT44_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ2534162.1"
                     /translation="MIDRQTNEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPLARLKQWLGMMKKEFAAAQNLFDRVRTVKDADEVRNILAEFEREMNT"
BASE COUNT          292 a          277 c          271 g          199 t
ORIGIN      
        1 ctacccaaat catgattgac agacaaacaa acgaacccaa acaaaaaacc cgaatcatcc
       61 ttgcccctat gcagggtctg gtcgatgacg tgatgcgcga cctgctgacg cgtattggcg
      121 gctacgacga atgcgtcagc gaatttgtac gcattaccca taccgtgcat tcccgatcca
      181 tatggttaaa atatgtcccc gaaatcgcca acggaaacaa aacgttttcc ggcacgccct
      241 gcaccgtcca acttttgggc agcgatgcgg acaatatggc ggcgaatgcg ctggaagccg
      301 tccgcttcgg tgcgaacaaa atcgatttga acttcggctg ccccgcgccc accgtcaaca
      361 aacacaaagg cggcgcaatc cttttaaaag agccggaact gatattccac atcgtcaaaa
      421 cgctgcgcgg acgtttgccc gcacatattc cgctcaccgc aaaaatgcgg ctcggttacg
      481 aagacaaaag ccgggctttg gaatgcgcct gtgcgattgc cgaagggggc gcatgcggac
      541 tgaccgtaca cgcgcgtacc aaagccgagg gttacgaacc gccggcgcat tgggaatgga
      601 taaggaaaat ccgagacagc gtcaatattc ccgttaccgc caacggcgac gttttcagcc
      661 tgcaagacta tatcggcatc aaaacaatca gcggctgcaa cagcgtgatg ctcggtcgcg
      721 gcgcggtcat ccgccccgat ttggcgcggc aaatcaagca atacgagaac ggcgggccgg
      781 tcaaagacac ggattttgcc gaagtttcca aatggatacg gcagtttttc gagctgtgcc
      841 tgacaaaaga ggcaaacaac aaatatccgc tggcgcggct gaaacagtgg ctgggtatga
      901 tgaagaaaga atttgcagca gcacaaaatc tgttcgaccg cgtccgaacg gttaaggatg
      961 cggacgaagt tcggaacatc ttggctgaat ttgagcgaga aatgaatact tgaatatgta
     1021 tagtggatta acaaaaacc
//
LOCUS       DAWGRZ010000266         1032 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.266, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000266 DAWGRZ010000000
VERSION     DAWGRZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.266"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(122..550)
                     /locus_tag="WHT44_002048"
     CDS             complement(122..550)
                     /locus_tag="WHT44_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ2534163.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KKSGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(531..965)
                     /locus_tag="WHT44_002049"
     CDS             complement(531..965)
                     /locus_tag="WHT44_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ2534164.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
BASE COUNT          262 a          316 c          190 g          264 t
ORIGIN      
        1 tttgaaaacc ccattaaacc gtctttaacc catcgtcccg aacggtagat gtttcacccc
       61 tgcaaaccaa aaaaaagacc gtctgaatac agacggccaa agcctggtca cacatcactg
      121 cctaaaataa agctgcctgc tgtgccgcag gtcggctcat ttcattgata atcgtatatc
      181 ccgttcgtga agagatgccg tatttagggc atagcttcgt catcgccata agcccgctct
      241 tcttatcaat atcgcgcaat ttgacaaact cctgataaaa cctatggttt ctcaactgta
      301 ttaacgcctt gccgcaccgt gggacataca attcctcgcc accatatacc tgcaacagct
      361 catgtgtttt cacttcgccg atggcttcga ccaaaattgc caaacgctcg gtgtccacct
      421 tgcccttacc aaatttaaac cgcgccccgc caatcgcctt gaccagctgt tccgtcgctg
      481 ccagtccgat gacatccaca atgtccaaca cggtatccgg caataaatgt tcaacttttt
      541 cgaaccccat catccccacc cgctttttcc gttttcctgt tttccgcaat ctgcaacgca
      601 gcaaccagtt tatgtagctg cgtatcgtct aaatattcga ccttatcctt accaaacatc
      661 cgccgcgcca ttgcgtgtgc atagttccaa tgtttgccgc cgacggtcag cagggcttcg
      721 actttgtcca acattgccgc tgatgatgtc cgacgcagat gcggtttgcc gtgtgggtta
      781 ccttttgctt taggcttaaa tccgtgcgac cgcatatcag cgacaacaga ctcaagttcg
      841 gaaacatcca tatccgcaca cgaccgcttg cccgtcacac gctccaacac cgcgcgatag
      901 gtaccgtcat ccaagcccag ctccttttga gcaatcttaa ttttcgcaat caacgcccgg
      961 cgcatctcaa acccataaaa cacaatatat agtattaagc cgatgttttt tgcgaaacgg
     1021 acagacataa aa
//
LOCUS       DAWGRZ010000267          998 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.267, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000267 DAWGRZ010000000
VERSION     DAWGRZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.267"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            35..418
                     /locus_tag="WHT44_002050"
     CDS             35..418
                     /locus_tag="WHT44_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ2534165.1"
                     /translation="MYFFPRFPFIDIDRIRWVCAGLKAFKHYIRENGLPDLIHAHCMN
                     YAGILAFKISQKYGIPYVVTEHSSTITRGLVRPHQWQPMKNAAAHAAARLAVSRHFAH
                     VLQHKYGCEWQYLPQHTGRNIQPTV"
     gene            367..>998
                     /locus_tag="WHT44_002051"
     CDS             367..>998
                     /locus_tag="WHT44_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ2534166.1"
                     /translation="MAVPPPTYWAEYSTDRLNKEKKNNKPHFVFCTVSHLRRLKGHDV
                     LLTAFARALAQCPQLRLNIGGSGQEEQRLKQQAADLGITHAVTFLGALQPEAVLDLMR
                     NSDAFVLASRTETFGVVYIEALSQGLPVIATRCGGAESIVSDGNGYLVPVDDDDALAD
                     ALIKMCEHHSDFEPARLRENCLNEFGENAVIGRLIGIFRQAIAEYGKKIPV"
BASE COUNT          248 a          294 c          244 g          212 t
ORIGIN      
        1 gcaaagcggt ttggacatct atgcatggca cggcatgtat ttcttccccc gctttccgtt
       61 catcgacatc gaccgcatcc gctgggtgtg tgcgggtttg aaagctttca aacactacat
      121 ccgcgaaaac gggctgcccg acctgattca cgcccactgt atgaactatg ccggcatact
      181 tgccttcaag atttcccaaa aatacggcat cccctatgtc gtcacggaac acagcagcac
      241 cattacgcgc ggtttggtgc gcccgcacca atggcagcct atgaaaaatg cggcggcaca
      301 cgccgccgca cgtctcgccg tcagccgcca tttcgcacac gtcctgcaac acaaatacgg
      361 ctgcgaatgg cagtacctcc cccaacatac tgggcggaat attcaaccga ccgtttgaac
      421 aaagagaaaa aaaacaacaa accgcatttc gtgttctgca ccgtctcgca ccttcgccgt
      481 ctcaaaggac acgatgtcct actcactgcc tttgcccggg cgttggcaca atgcccgcaa
      541 ctgcgcctga acatcggcgg cagcggacag gaagaacagc ggctgaaaca gcaggcggca
      601 gacttgggca ttacccatgc cgttacattt ttgggcgcat tgcagcccga agcagtcttg
      661 gatttgatga ggaacagcga cgcattcgtc cttgccagcc gcacagaaac cttcggcgta
      721 gtctatatcg aagcactgtc ccaaggattg cccgtcattg caacacgctg cggcggtgcg
      781 gaatctattg tttcagacgg caacggatat ttggttcctg ttgacgacga cgatgccctt
      841 gccgacgcac tcatcaaaat gtgtgaacac cactctgatt tcgaacctgc ccgacttaga
      901 gaaaactgtc tgaacgaatt tggcgaaaat gccgttatag gcaggttgat cggcattttc
      961 cgacaggcaa tcgcagaata cggtaagaaa ataccggt
//
LOCUS       DAWGRZ010000268          991 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.268, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000268 DAWGRZ010000000
VERSION     DAWGRZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.268"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..387)
                     /locus_tag="WHT44_002052"
     CDS             complement(<1..387)
                     /locus_tag="WHT44_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ2534167.1"
                     /translation="MFIILGRNFLKIILCFSFFVSKFALASVNAPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGVFDRESGRYLTSEAQDLKVRHVSTGASSTGKVSSVVSSSVS
                     RAGVLAGVGKLARLGAKLSTRAVPYVG"
     gene            complement(734..>991)
                     /locus_tag="WHT44_002053"
     CDS             complement(734..>991)
                     /locus_tag="WHT44_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534168.1"
                     /translation="ADDYLLSSLPPTVTQDGKIIRPERVGDKWILNGKPVTLSYPECS
                     NFEQIKQGSYVGSTVLILFVVIYGFRLLINFLKDIGKVGTD"
BASE COUNT          359 a          222 c          139 g          271 t
ORIGIN      
        1 tccgacataa ggaactgccc ttgtgcttaa tttcgcgcct aagcgggcaa gtttgccgac
       61 ccccgccaat acgccagcgc gggaaactga tgaagataca accgaactaa ctttacccgt
      121 acttgatgct ccagtagata catgcctaac ttttaaatct tgagcttctg aagttaaata
      181 tcttcccgat tctctatcaa atacaccttt ccaaattctt cttcttttgt catctgaacc
      241 tcgaatacct aaatatctgc catcatcata aacttcaacc ctatcaaatt tacccggagc
      301 atttactgat gccaatgcaa atttagatac aaaaaaacta aagcatagga taatctttaa
      361 aaaatttcgc cctaggataa taaacataac attttgcttc atctaattta attaataatg
      421 tagaagaatc agggaattga atttctataa aatctctatc ataacgatga aagaaattcc
      481 taaaaatatc aaatgaaaac gaagttttaa aaaccccttt tctaatcaat tccctataat
      541 aaattaacgc ttgtggtaaa tctctataaa tatgtgaatc agtttgaaat ttaactaaat
      601 gatattcaga aataaacata attcggacct tttaaaacac tctaaaaagc cgattctacc
      661 aaccgttacc aaaacagcca agcaacagac aaagccaaga agaaaccgag aagaaaccag
      721 aaatctataa tcatcaatca gtcccaacct tgcctatgtc ttttaaaaaa ttaatcagaa
      781 gcctgaaacc gtaaatgact acaaacagaa ttagaaccgt cgaaccgaca taagaacctt
      841 gctttatctg ctcaaaattg gaacattccg gataagacaa cgtaaccggc tttccgttca
      901 aaatccattt atcgcccacc ctttccggcc tgatgatttt tccgtcctgg gtaacagtag
      961 gaggaaggga cgacaataaa tagtcgtctg c
//
LOCUS       DAWGRZ010000269          966 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.269, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000269 DAWGRZ010000000
VERSION     DAWGRZ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.269"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(137..769)
                     /gene="mtrR"
                     /locus_tag="WHT44_002054"
     CDS             complement(137..769)
                     /gene="mtrR"
                     /locus_tag="WHT44_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ2534169.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          223 a          209 c          250 g          284 t
ORIGIN      
        1 agattgagca taaaatttta gtaacctatg ttattgcaaa ggtctcaata tgtattttta
       61 gaagaaggct ttgtgtcatt ttgatgccgt ctgaaagggt ttgttcgttt cagacggcat
      121 tgctaccaag gcttgattat ttccggcgca ggtcgggatg gttttccaag ttgtccatca
      181 ttatcccgat gatgcgcggg gcggttttgc ccaaatcgaa acgttcgcag gaagagaacc
      241 accgccaaat cagcccgtcc aaggttgatt tgatgaagat aactgccgtt tccttgtcca
      301 aatcgtcagc caaatcctga ttttccaccg cttcggtcaa aacggcggta attttctcgc
      361 gccagattgc ctgatgcttg cgggcaatgg cgataacggc ggcgttttgc tccgtgtgtt
      421 cgcattttaa aaacaggatg ttgtggaatt tgtagtagat gtcgttgctt tgcagccgct
      481 cgaaaaagtg cagcagcgtg tggcggaata ccgcccaaga ccctccttcg gcatcttcgg
      541 catcttgcgc gatgcagttt tcgatgtcgt cgcagatacg ttggaacagc gcgtcgaaca
      601 agtcttcctt atttttgaaa tgccaataga gcgcgccgcg cgttacgccg gcggcttggg
      661 cgatttcgtt gagcgaggtg cgcgcaatcc ctttgcggta aaaggtttcc aaggcggcaa
      721 gcatcaggtg ttctttggtt tttaaggctt cggttttggt ttttctcata atggcggttt
      781 cgtttcgggt cggtttgatg agggcggatt ataaaaaaga ctttgtaacc atgcaatcgt
      841 gtatgtataa tgaaacccat gaaattgaga ctacatctca actttgaaaa cccatgaaac
      901 ctgcttcgca acccgtttga acatcgggtt ggcgaagcag gcggttttta tatcctgaaa
      961 tatagt
//
LOCUS       DAWGRZ010000270          961 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.270, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000270 DAWGRZ010000000
VERSION     DAWGRZ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.270"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..240
                     /locus_tag="WHT44_002055"
     CDS             <1..240
                     /locus_tag="WHT44_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534170.1"
                     /translation="DGSNIDTCSVLVATPLPAQSGNAVGFTAAQMKFGDSKNFSKLEN
                     LKYPCEVMVTVEMTSTGKGMVPSLIDFQVAEKPKG"
     gene            244..444
                     /locus_tag="WHT44_002056"
     CDS             244..444
                     /locus_tag="WHT44_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534171.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEIGGGFQIFQFFVKSE"
     gene            681..911
                     /locus_tag="WHT44_002057"
     CDS             681..911
                     /locus_tag="WHT44_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534172.1"
                     /translation="MNIVKKYAVKAALAAGIFTPAIVMADTFDPSAIGTQVANVIMGF
                     VSMVSAVGMAAITVILAIQGFKMAWSMIKSVK"
BASE COUNT          300 a          176 c          223 g          262 t
ORIGIN      
        1 gatggctcta atatcgacac ttgttccgta ttggttgcaa cacctttgcc ggcacagtcg
       61 ggaaatgctg ttggattcac ggcagcacaa atgaagttcg gggacagtaa gaatttctca
      121 aaattagaga atctcaaata cccgtgcgaa gttatggtaa cggttgaaat gacttcgaca
      181 ggtaagggca tggttccttc attaattgat tttcaggtgg cagaaaagcc gaaaggttga
      241 tttatgaaat ttgaagaacg tttcatagtt caagacttgg aaacgcatga ctttatttat
      301 cccgatcctt tcggtgatgt ggggtttact caaaatatta aatcagcagg tcaatttgaa
      361 agctacgaag atgcgttgaa ttcaggcata aatgaaatag gcggaggatt ccagatattt
      421 cagttcttcg taaaatcgga ataaaagaaa aacaggctcg gcgggcggtc tgtcaacctt
      481 tcacaaagcc cgcaacaaag gaaaaatatc atgaaaatga accttgcaac actaattatc
      541 ggctgggtgg tctgtatgtt tctttttctt ttcgcaatcc tctattttat cggctaaaaa
      601 cgagattcgg aaaagacttc gtccggatga agcaagtcaa gaagtcgtct tattttaaat
      661 atcaaaaaag gaaaaaaacg atgaacatcg ttaaaaaata cgctgtaaaa gcagccttgg
      721 cagccggtat cttcacaccg gccattgtta tggcagatac ctttgatcca tccgcgattg
      781 gtacgcaagt agcgaatgta atcatgggtt tcgtgtcaat ggtttccgcc gtgggtatgg
      841 cggccattac cgtgattctt gcaatccaag gcttcaaaat ggcttggagc atgattaaat
      901 ctgtcaaata aacagagtga agaaaaaggg gcgtataaat gggctatcgt gtcggcataa
      961 a
//
LOCUS       DAWGRZ010000271          906 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.271, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000271 DAWGRZ010000000
VERSION     DAWGRZ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.271"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..216
                     /locus_tag="WHT44_002058"
     CDS             <1..216
                     /locus_tag="WHT44_002058"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534173.1"
                     /translation="ADAGDVYCVYNIRLKQYTACQVTRVEEHGGKKTHATLLALDWQG
                     NKPLGAEELADLKPLYKDLKNNIGNIV"
     gene            547..>906
                     /locus_tag="WHT44_002059"
     CDS             547..>906
                     /locus_tag="WHT44_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534174.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLY"
BASE COUNT          282 a          147 c          212 g          265 t
ORIGIN      
        1 gctgatgccg gagacgtata ttgcgtctat aacatcagac tgaagcagta cactgcctgc
       61 caggttaccc gagttgaaga acacggtggc aaaaaaacac atgcgaccct gctggctttg
      121 gactggcagg gcaacaaacc gcttggggcg gaggagctgg cggatttgaa accactttac
      181 aaagacttaa agaataatat tggaaatatt gtatgaacaa aaaattaaac tatattttta
      241 tgttggactg tttagggttg gtgatattgt ttacttgtat aatagctact tttgaaagag
      301 attatggatt taaaattttt actaattcta agaggcctga attttattat tggattggaa
      361 tgttttatta tggaattatt tcttgctggt ttgattatca attaatttca acaaaggcga
      421 attcgtataa aagaaaagtt aaacaatata aaattttttc agtaatattt tcagttttga
      481 tatttatttc tactatagta aaactttaaa ttttggagca aaaatttatg agcgattcaa
      541 ttgaatatgt attgggaacg cggtctgcac atgtataagg caagtgctgt cgtgccaacg
      601 ggatatgtac gggttgggaa taccgcgccg ctggtcggcg aagacacgca acggtatgcc
      661 tctttttggg gcgacggcta cgacgtgtac cgtcagttga gatggcagca gatacccgaa
      721 aaacagagaa aggcattcaa aaaagccgcc aaaagcaaaa agaccgtgat gtttgccgga
      781 cgggaatacg gcatatccaa acagaatttg agcgatgttt gggatgattt tgaagacgcg
      841 atggaactga aggcgtttcc ctgcctgtct tcgctgtttc tgaccaagtg gcataaaaat
      901 ctatat
//
LOCUS       DAWGRZ010000272          901 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.272, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000272 DAWGRZ010000000
VERSION     DAWGRZ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.272"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(170..328)
                     /locus_tag="WHT44_002060"
                     /pseudo
     CDS             complement(170..328)
                     /locus_tag="WHT44_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            484..>901
                     /locus_tag="WHT44_002061"
     CDS             484..>901
                     /locus_tag="WHT44_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534175.1"
                     /translation="MNKTLYRVIFNRKRGAVVAVAETTKREGKSCADSDSGSAHVKSV
                     PFGTTHAPVCRSNIFSFSLLGFSLCLAVGTANIAFADGIIADKAAPKTQQATILQTGN
                     GIPQVNIQTPTSAGVSVNQYAQFDVGNRGAILNNSRS"
BASE COUNT          281 a          182 c          176 g          262 t
ORIGIN      
        1 aaaatgctct ttgggaatgc tattggatta tagaccttac aacagaaaaa aaggtcggaa
       61 agtagaaaga ctgcttttta aattcaaacg gccaagcaag taaagacttg gccaacaaat
      121 gggacaaaat ccgactgttt taaattgcca gtcaaatact ggaaaacttg cgtaaatggc
      181 taaattcact gacatcaagt acatcataac tacgaaaggt atccgtatac acaatgccat
      241 cagacttaac tttctctcgg ataatcggca ataatgttgc tgattgcgca ttagggacaa
      301 cgacggtata aaccttgcca tttcgttcga atgggatttt taaccgttat ctagtaaagt
      361 ccctaattta ataaacaaat ggcagtaaaa tggcggtaat tgactcgcca aaatccttga
      421 tctagaatgg tcagactatc caattttttc taataactct ataaaaatca aataactgaa
      481 atcatgaata aaactctcta tcgtgtaatt ttcaaccgca aacgtggggc tgtggtagcc
      541 gttgctgaaa ctaccaagcg cgaaggtaaa agctgtgccg atagtgattc aggcagcgct
      601 catgtgaaat ctgttccttt tggtactact catgcacctg tttgtcgttc aaatatcttt
      661 tctttttctt tattgggctt ttctttatgt ttggctgtag gtacggccaa tattgctttt
      721 gctgatggca ttattgctga taaagctgct cctaaaactc aacaagccac gattctgcaa
      781 acaggtaacg gcataccgca agtcaatatt caaaccccta cttcggcagg ggtttctgtt
      841 aatcaatacg cccagtttga tgtgggtaat cgcggggcga ttttaaacaa cagccgcagc
      901 a
//
LOCUS       DAWGRZ010000273          880 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.273, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000273 DAWGRZ010000000
VERSION     DAWGRZ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.273"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(187..507)
                     /locus_tag="WHT44_002062"
     CDS             complement(187..507)
                     /locus_tag="WHT44_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ2534176.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMALIASKEFEEFVRNVKE
                     YDVIECFSYFLNKRVTHNIWKIYFSDESNIFIRKSEENGKISHEFIYSEFSDSNTDFN
                     VLFS"
     gene            complement(626..>880)
                     /locus_tag="WHT44_002063"
     CDS             complement(626..>880)
                     /locus_tag="WHT44_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534177.1"
                     /translation="ADDYLLSSLPPTVTQDGKIIRPERVGDKWILNGKPVTLSYPKCS
                     NYEQVKSGAYLGSMVLILFVVIYGFRLLINFLKDIGKVGA"
BASE COUNT          279 a          182 c          145 g          274 t
ORIGIN      
        1 acgctggcgg ttatctttgc gttgattcgg gcttttgcgc ccgtggggat atgttcgaca
       61 tttgccgctt ctgtgaattt agaaaattca ttattgattt ttctatatcc tgtagattca
      121 aaaaacttta atttagatgg cctaaatttt attattcgat catctattct tgctggttct
      181 gcaaaactaa gagaacaaca cattaaaatc ggtgttgcta tcagaaaatt ccgagtaaat
      241 aaattcatgc gaaatttttc cattttcttc tgacttccta ataaaaatat tagactcatc
      301 agaaaaataa attttccaaa tattatgcgt taccctctta tttaaaaaat aagagaaaca
      361 ttctattacg tcgtattctt ttacatttct tacaaactct tcaaattcct tagacgcaat
      421 taatgccatc gactgcccaa aatacttgct ggacggctga tatttataaa gtgccaactg
      481 cgcctgcgtg ataaacggct tgttcatgtt tctgtctttc aaaggttgtt ttgaaagcct
      541 gattttaaaa cacgtcatat aaatatcaaa gcgacagaca aagccaggaa aaatccgagc
      601 aaaaaccaaa aatcgacaaa catcatcacg cccctacttt gcctatgtct tttaagaaat
      661 taatcagcag cctgaagccg taaataacga caaacagaat taaaaccata gacccgagat
      721 aagctccgga tttaacttgt tcgtaattcg aacatttcgg ataagacagc gtgaccggct
      781 ttccgttcaa aatccattta tcgcccaccc tttccggcct gatgattttt ccgtcctggg
      841 taacagtagg aggaagggac gacaataaat agtcgtctgc
//
LOCUS       DAWGRZ010000274          854 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.274, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000274 DAWGRZ010000000
VERSION     DAWGRZ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.274"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            24..>854
                     /locus_tag="WHT44_002064"
     CDS             24..>854
                     /locus_tag="WHT44_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ2534178.1"
                     /translation="MNEGNQLEVKGNGQYSTIKDKDREREYTYHQGRGGGGSVSFNNS
                     DELVSRQSGTAVFGTATYLPPYGKVSGFDERKLKERTNALNWIHTTHPGLIGYSYAGV
                     VCRDSTGCPKLVYKTQFTFGNSGLAKKANGGGLDIYVDKSRDNSPIYKLKDHPWLGVS
                     FNLGSENTVKNSKSSNKLISSFSEDNNNQTIVSTTEGSPISLGDQQREHTAVVYYLNA
                     KLHLLDKKQIQNITDKTVQLGVLKPSIDVRQQKGANWLSFWASWDIKDTGQIPVKLGL
                     Q"
BASE COUNT          263 a          200 c          202 g          189 t
ORIGIN      
        1 aaacctataa atacgctatt atcatgaacg agggaaacca gctcgaggta aaggggaatg
       61 ggcaatattc aacaataaag gacaaagaca gggaacgcga atatacttat catcagggca
      121 gaggaggagg aggctctgtc tcattcaaca atagcgatga gcttgtttct cgacaaagcg
      181 gtactgccgt ttttggcaca gccacctacc tgccgcccta cggcaaggtt tccggttttg
      241 atgaaagaaa gctgaaagag cgcaccaatg cccttaattg gattcatacg acccacccag
      301 ggttgatagg ctacagctac gccggtgtcg tatgcagaga cagcacaggc tgtcccaaac
      361 ttgtctataa aacccaattt accttcggta attccggttt ggcaaaaaag gcaaacggcg
      421 gcgggctgga tatatacgta gacaaaagcc gcgacaattc gcccatttac aaattgaaag
      481 atcatccatg gttgggcgta tctttcaatt tgggcagcga gaataccgtc aaaaatagca
      541 aatcatccaa caaattgata tcttctttta gtgaagacaa taataatcaa accatcgtct
      601 ctacgacaga aggctcccct atttcccttg gcgaccagca gcgcgaacat accgccgtgg
      661 tctattatct gaacgccaag ctgcacctgc tggataaaaa acagattcaa aatatcaccg
      721 acaaaacagt gcagttgggt gtcttgaagc cgagcatcga tgtgcggcag cagaaaggtg
      781 ccaactggct aagtttttgg gctagttggg acattaaaga taccgggcag attccagtca
      841 agctcggcct gcag
//
LOCUS       DAWGRZ010000275          776 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.275, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000275 DAWGRZ010000000
VERSION     DAWGRZ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.275"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            <1..>776
                     /locus_tag="WHT44_002065"
     CDS             <1..>776
                     /locus_tag="WHT44_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ2534179.1"
                     /translation="PGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQV
                     GVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALKALEGDAA
                     YEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERGIIHVGDE
                     IEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVLAKPGTIT
                     PHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGA"
BASE COUNT          196 a          223 c          203 g          154 t
ORIGIN      
        1 ccggggcacg ccgactacgt taaaaacatg attaccggcg ccgcacaaat ggacggtgca
       61 atcctggtat gttccgcagc cgacggccct atgccgcaaa cccgcgaaca catcctgctg
      121 gcccgccaag taggcgtacc ttacatcatc gtgttcatga acaaatgcga catggtcgac
      181 gatgccgagc tgttggaact ggttgaaatg gaaatccgcg acctgctgtc cagctacgac
      241 ttccccggcg atgactgccc gattgtacaa ggttccgcac tgaaagcctt ggaaggcgat
      301 gccgcttacg aagaaaaaat cttcgaactg gctgccgcat tggacagcta catcccgact
      361 cccgagcgag ccgtggacaa accgttcctg ctgcctatcg aagacgtgtt ctccatttcc
      421 ggccgcggta cagtagtaac cggccgtgta gagcgcggta tcatccacgt tggtgacgag
      481 attgaaatcg tcggtctgaa agaaacccaa aaaaccactt gtaccggtgt tgaaatgttc
      541 cgcaaactgc tggacgaagg tcaggcgggc gacaacgtag gcgtattgct gcgcggtacc
      601 aaacgtgaag acgtggaacg cggtcaggta ttggctaaac cgggtactat cactcctcac
      661 accaaattca aagcagaagt atacgtactg agcaaagaag agggtggtcg tcacactccg
      721 ttcttcgcca actaccgtcc gcaattctac ttccgtacca ccgacgtaac cggcgc
//
LOCUS       DAWGRZ010000276          758 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.276, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000276 DAWGRZ010000000
VERSION     DAWGRZ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.276"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
BASE COUNT          225 a          193 c          189 g          151 t
ORIGIN      
        1 agaaaccctc gaccgtcatt cccgcgaacg cgggaatcca gtaaccgaaa aaccacagga
       61 atctatcgga aaaaacagaa ccccccgacc gtcattcccg cgaacgcggg aatctagaac
      121 gtagaatctg agaaaccgtt ttactcgata aatttccgtg ccgacaggtc tggattcccg
      181 ccttcgcggg aatgacggca tcagtttgca ggattcggcg gaaacggtaa aaacggcaga
      241 atcgatggga tgcggcaggc tgaagcccac caaaacacaa aaattccgat gccgtctgaa
      301 atttcgtcat tcccgtgaaa acgggaatcc agccccgtgg gagcagaaac ttacaccccg
      361 tcattcccgc aaaagcggga atccagtaac cgaaaaacca cgggaatcta tcggaaaaaa
      421 cagaaccccc cgccgccgtc attcccgcga acgcgggaat ctagaacgta gaatctgaga
      481 aaccgtttta ctcgataaat ttccatgccg aggggtctgg attcccgcgt tcgcgggaat
      541 gacggcatat tttttgcatt tgatataaag ggtcgtttga attttgttca gcaagtgcaa
      601 agtgttgcac ataaaagggc gcaggataga ggcaaagcgg gcgtaggtcg ggctgtagca
      661 actgtatttt tcaccccgtc gggcaaaaat atagtggatt aacaaaaacc agtacggcgt
      721 tgcctcgcct tagctcaaag agaacgattc tctaaggt
//
LOCUS       DAWGRZ010000277          748 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.277, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000277 DAWGRZ010000000
VERSION     DAWGRZ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.277"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(27..422)
                     /locus_tag="WHT44_002066"
     CDS             complement(27..422)
                     /locus_tag="WHT44_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534180.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASVSRAGVLAGVGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDPETDKFAKVSG"
     gene            complement(406..723)
                     /locus_tag="WHT44_002067"
     CDS             complement(406..723)
                     /locus_tag="WHT44_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ2534181.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMAVIAQSEFVEFAKINKS
                     ENVIDCFSFFWNRRIKHDIWLISFSDNSEMVIKESLKDGHKIYKFEFCEIVDNCNFDD
                     VFV"
BASE COUNT          199 a          154 c          148 g          247 t
ORIGIN      
        1 taccttttag gcggcaacag gcgcacttag cctgagacct ttgcaaattt gtcggtttcg
       61 gggtcgtatt ggtagcctcg tgcctgtatg tcttctttga aagtttcgta tacgtcgtgg
      121 gctaaaaggg ctgttccgac atagggaacc gcccttgtgc tgaatttcgc gcctaagcgg
      181 gcaagtttgc cgacccccgc caatacgccg gcgcgggata cgctggcggt tattttggcg
      241 ttgattcggg cttttgcgcc cgtagggatg tgtgttaaat ctaccgtttt tattaaatca
      301 gatgaataag ttttactatt tttaggtaca aacttatgaa ttttcgcacc ttgtccggta
      361 tcaactgaaa cagtttcaga tatttttact gcatttgcat tcgcttcaaa cgaatacatc
      421 atcaaaattg caattatcga caatttcgca aaattcaaat ttgtatattt tatgaccatc
      481 tttcagggat tctttaatta ccatttctga attatcagaa aatgagatta gccaaatatc
      541 atgtttaatt cttctattcc agaaaaaaga gaaacaatca ataacatttt cagacttatt
      601 aatcttcgca aattcaacaa attcagattg cgctataacc gccatcgatt gcccaaaata
      661 cttgctggac ggctgatatt tataaagtgc caactgcgcc tgagtgataa acggcttgtt
      721 catggttctg cctttcaatg attgtttt
//
LOCUS       DAWGRZ010000278          726 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.278, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000278 DAWGRZ010000000
VERSION     DAWGRZ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.278"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(129..>726)
                     /locus_tag="WHT44_002068"
     CDS             complement(129..>726)
                     /locus_tag="WHT44_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ2534182.1"
                     /translation="LSIGQIVKVKPAGYAAPKTAAVESRPAVPAAVQTPVKPAAQPPV
                     QSAPQPAAPAAENKAVPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGV
                     DIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQV
                     KRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSYIAF"
BASE COUNT          166 a          210 c          193 g          157 t
ORIGIN      
        1 ccaaacacag tcaaacaaat ttatatgccc catcccttcc gaataatttg aaaacacagc
       61 cgccaaaaac aaaaatgccg tctgaaaacc tttcagacgg catttccaac ttgatttcag
      121 gcagaaagtc agaacgcgat atagctgttc gggttaaccg gtttgccgtt ttgacgcacc
      181 tcgaaatgaa gctgcgttct ggaagcatcg gtattgccca tcaaagcaac ctgctgaccg
      241 cgtttgacct gctgcccctc gccgaccagc aatttttggt tgtgcccgta tgcggtcagg
      301 aaagaagaat tatgctggat gatgaccaag tttccgtatc ccctcaaacc tgaaccggca
      361 taaaccactt tgccgtcagc cgccgccaaa acgggctgtc ccgcattacc ggcaatatcg
      421 acacccttgt tgttgccgcc gaaatcggca accactttac cttgcgtcgg acgctgccaa
      481 acaatgccgc cgaccgaacg cgtgccggaa ggcgaagcgg caggagattg cggggcgggc
      541 gcgggaaccg ctttattttc cgcagcgggc gcggcaggtt gcggcgcgga ctgcacaggc
      601 ggttgcgcgg cgggtttcac aggggtttgc acggcagccg gtacggcggg cctgctttct
      661 acggctgcgg ttttcggtgc ggcatatcct gccggtttga ctttaacaat ctgaccgatg
      721 ctcaac
//
LOCUS       DAWGRZ010000279          706 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.279, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000279 DAWGRZ010000000
VERSION     DAWGRZ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.279"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(90..641)
                     /locus_tag="WHT44_002069"
     CDS             complement(90..641)
                     /locus_tag="WHT44_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534183.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
BASE COUNT          198 a          277 c          134 g           97 t
ORIGIN      
        1 caaagaaacc gccattagca ctacaccccc caagctgcgg ctgccaaaca catcggctgc
       61 ttctcgacgc acccgctgaa aaatgcgtcc tatccgtccg ccttgcccgc accccctgac
      121 tttgccgccc tgcccgatgc cgtctgaagc ctgaaccgcc ccaccagccc gatgacgcac
      181 gacaccgccg gaaagggcat acacaaagcc gtcagcacat aacgcgcatt ccccgtattg
      241 accacgtccg cgcccttcag cagcagcggc agcgcgttcg cccactccca caccgacggc
      301 gcgctgaacg ccaataccgc caccagcccc aacatccgga acaccccgat ttccaacacc
      361 tgccgccgcc gcaccgaacg gttgagcaaa aacacaaccg ccaacccgaa acagaccacc
      421 gccgccatac cgccgttggc gacaatctgc aaactgttga atgcaatatc gggcgtaacc
      481 gccaacacgc cttccgaatc cggcacggca cgctgcaaat ccaaaccctg caacacattc
      541 aacacaaaac cgtagatcaa atcaaaaaac accacgccta tcggcaaagc actcaaaacc
      601 cgccacatct tcctgtccga ccaaccgttc aaaactttca ttttacccga ccacgcaagc
      661 cgccgaacaa aaaacaaggg gctgtcctag ataactagga caaact
//
LOCUS       DAWGRZ010000280          688 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.280, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000280 DAWGRZ010000000
VERSION     DAWGRZ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.280"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            232..531
                     /locus_tag="WHT44_002070"
     CDS             232..531
                     /locus_tag="WHT44_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ2534184.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFFFAALTVLKLKGYQAGLYTLLIALAVAVFGFGMPTGMAVSSLPPQPH"
     gene            528..>688
                     /locus_tag="WHT44_002071"
     CDS             528..>688
                     /locus_tag="WHT44_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tonB dependent receptor family protein"
                     /protein_id="HEZ2534185.1"
                     /translation="MTQRPYATLYLTAHYKIGKSTRIGLDFENVFNKRYRPMPDIHVY
                     GTPRSLTATV"
BASE COUNT          178 a          197 c          142 g          171 t
ORIGIN      
        1 tcaaacatca aattggactg accaatcagg gcggattcta atgacacgcc gttcccgccg
       61 tcaacggcat ttacctcgca ccgcccccga aacacaagaa aaaactacac caaactacaa
      121 tttttgttca tgcaaatatt tgttttgaca ggatttaaac aaaagctccg attcaaatct
      181 gccgaaccgc ccaaaaatat attgacctaa atattaaagt ttcgtaaagt aatgcaacgt
      241 tgctttaatt ggtttgacca ctattgccga cgattagaaa aatattttcg gagatgttca
      301 attatggaaa cttgggttca aaactacacg gcaatcggcg gcagcctgta tctgactgcc
      361 gccgccgcac tcttacccat cgtctttttc tttgccgcgc tgaccgtcct gaagctgaaa
      421 ggctatcagg cggggcttta tacgctgctg attgcgcttg ccgttgccgt attcggcttc
      481 gggatgccga cgggtatggc ggtttcttcc ctgccgccgc agccgcattg acccaacgcc
      541 cctacgccac actgtatttg accgcgcatt acaaaatcgg caaatccacc cgcatcggtt
      601 tggactttga aaacgtgttc aacaaacgct accgccctat gcccgacatt cacgtttacg
      661 gcacgccgcg cagcctgacc gcaaccgt
//
LOCUS       DAWGRZ010000281          677 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.281, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000281 DAWGRZ010000000
VERSION     DAWGRZ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.281"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            4..236
                     /locus_tag="WHT44_002072"
                     /pseudo
     CDS             4..236
                     /locus_tag="WHT44_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212266.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            295..603
                     /locus_tag="WHT44_002073"
     CDS             295..603
                     /locus_tag="WHT44_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534186.1"
                     /translation="MTPWADAYADLRGKTKVMTTQMGASRDVSKSAKGWSVGIGLNVG
                     KQLTDSVGLEFDPYYRHKTIYKPREIVLDGDKTKMGRSKSNEYGFRVAATFYSQLKSK
                     "
BASE COUNT          211 a          164 c          153 g          149 t
ORIGIN      
        1 aaaatgttga aaacaaaaag aactcattgc ttgaaaaaag atttaatggg aatgcgggta
       61 tttcccacac tttttccatt tatttatcct cattatagga agaaaaacat gaaaaaaccc
      121 tgcttgcact ctcacttgca gccgtgtccg ccgttacggt agccgatatt cccgagtcta
      181 acggcaaagg caaagccatc atcggcgata aggaaaaaca ccgttaccaa gcatgaaaac
      241 cttgctttga aagtccatgg cgaaatccgc caagatttgg gggacggatg atccctgacc
      301 ccttgggcgg atgcatatgc agatttgcgc ggcaaaacca aagtgatgac gacccagatg
      361 ggtgcttccc gcgatgtcag caaaagcgcc aaaggttgga gtgtcggtat cggtctgaat
      421 gtaggcaaac agttgaccga cagcgtcggt ctcgagtttg atccatacta ccgtcacaaa
      481 acaatctaca aaccccgtga gattgtcttg gacggtgaca aaaccaaaat gggccgctcc
      541 aaatccaacg agtacggctt ccgcgtagcc gcaacgttct atagtcaatt aaaatcaaaa
      601 taggacagta gtgcatcgtc aaatcgggcg taatcagaca aaacggttcg cagataccgc
      661 ttaatattcg cccacac
//
LOCUS       DAWGRZ010000282          662 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.282, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000282 DAWGRZ010000000
VERSION     DAWGRZ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.282"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(60..545)
                     /gene="fxsA"
                     /locus_tag="WHT44_002074"
     CDS             complement(60..545)
                     /gene="fxsA"
                     /locus_tag="WHT44_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ2534187.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          195 a          212 c          120 g          135 t
ORIGIN      
        1 actatatttt caaaccggaa aaatgccgtc tgaaccttca gacggcattc cattcatatt
       61 tattcgtctt ttttgtgttc gatggcgttt cgggaacgat tgccgccgta aggctcttca
      121 accgtatatt ctccctcgat aatatcgtca tcgcgggaaa agccctcttt tctgcccgat
      181 tggttcatgt tgaaaaaatt ttccgcacct cctgcctgca acactgcccc tcccttaaac
      241 ggcagcagca gcaataccgc caacaccgag gatacgaatc ccggactcat cagacacaca
      301 gccgccaccg tataacggat aggccacaac atctgataaa cggataccct cccgccgctt
      361 ctcattgccg cgcccgccaa taaaagaccg gacagccccg tatgcctgag catcagcacg
      421 ccggcggcaa aacctgccgc catcaaaaac aacgtccagc cgccgcccag ccaatcggca
      481 acccacacaa tcgacataat ctccaaaaac agcagcacca aaaaaccgat accgaaaaat
      541 ctcattgacc gtcatcctta tatttaagta aacagcaaac cgcccgaaca ggactccaag
      601 cgagctgcct gtaaatgatt acaaaaccat gtgcttcaag ccgaaacaat gtgaaatcac
      661 gc
//
LOCUS       DAWGRZ010000283          585 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.283, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000283 DAWGRZ010000000
VERSION     DAWGRZ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.283"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            100..387
                     /locus_tag="WHT44_002075"
     CDS             100..387
                     /locus_tag="WHT44_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ2534188.1"
                     /translation="MDDLIAAFLGYLSGKYREGKISRLGISLTAGSIFFAVFFIHELI
                     IFIVDGNKENLWNFITEMMKDLFLFSCSVCLLFYFLLFIAETIGKTINKIK"
BASE COUNT          199 a           70 c           99 g          217 t
ORIGIN      
        1 ccaaagaggc tgaagggtaa gagacgctta tatcatagat atagtggcaa aacaggtaat
       61 gaattaaaga cattacctgt ttttacagaa atttttaaaa tggatgattt gattgcagct
      121 tttttaggct atttatcagg taaatataga gaaggaaaaa taagcaggtt aggaatatcc
      181 ctaacggcag gaagcatttt ctttgcagta ttctttattc atgaactgat aatttttatt
      241 gttgatggaa ataaggaaaa tctttggaat tttattactg agatgatgaa agatttattt
      301 ttattttcat gttctgtttg tttattattc tacttcttat tatttattgc ggaaactatt
      361 gggaaaacaa tcaataaaat taaataagat atttaagccg ctaaaaacca tttaagtatt
      421 ttatcttcta tgcggcattt atcttggaat tgaaatactg caatcgtgag gctaaggctt
      481 ccgttgtaac gtttaccata tatttcatta ttttaatttc atcaaaaaac aaaattcaag
      541 attatacata ccgccaatag tttcttgatt gttttgtata gtgga
//
LOCUS       DAWGRZ010000284          541 bp    DNA     linear   BCT 18-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M28424
            SAMN09011466-rid17423323.denovo.284, whole genome shotgun sequence.
ACCESSION   DAWGRZ010000284 DAWGRZ010000000
VERSION     DAWGRZ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09011466
            Sequence Read Archive: SRR7106593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-MAR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001659850.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001659850.1
            Genome Coverage       :: 190x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/13/2023 19:13:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,076
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,938
            CDSs (with protein)               :: 1,938
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 39 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 17 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09011466-rid17423323.denovo.284"
                     /isolate="M28424"
                     /isolation_source="Sterile Site"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA"
                     /collection_date="2012"
                     /collected_by="US State Health Department"
     gene            complement(<1..185)
                     /locus_tag="WHT44_002076"
     CDS             complement(<1..185)
                     /locus_tag="WHT44_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ2534189.1"
                     /translation="MYALSEKYNDNGFKAYKVLGEGGGIHTEYNYKFDKSLNLNVLES
                     STGARSLEKVPVKVTASV"
BASE COUNT          201 a           91 c           61 g          188 t
ORIGIN      
        1 actgatgcag ttactttaac ggggactttt tcaagagagc gtgcgcctgt tgaactttct
       61 aatacattca aattcaaact tttatcaaat ttgtaattat attctgtatg aattcctcct
      121 ccctcaccta aaactttgta tgccttaaat ccattatcgt tatatttttc cgaaagtgca
      181 tacatcaatt tcccattttt aatttctaaa tctgctgaaa aagatttagc actcaaaaga
      241 aacagaaaag aaattattag aatccgaaac atcaattttt ccattgccaa taatgaaaaa
      301 tgaatcatcc ttgaatttaa tttcaaagca agattcatta aaattaattc tattaaaaaa
      361 attatgacat ttatccattg agaaattttt caatagacca gtttctttca aataacaata
      421 aaacacataa gaaagaggcc tatcaggata agattctgac aatttatcta aatcagaatt
      481 tgatatatag aaaaagtcac gttcattttt attcatggtt tcaagcctca aatgtctaat
      541 t
//
